LOCUS       DAXHTR010000001        40643 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.001, whole genome shotgun sequence.
ACCESSION   DAXHTR010000001 DAXHTR010000000
VERSION     DAXHTR010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 40643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40643
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.001"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(171..863)
                     /locus_tag="ACFNML_000001"
     CDS             complement(171..863)
                     /locus_tag="ACFNML_000001"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6391469.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(971..2164)
                     /locus_tag="ACFNML_000002"
     CDS             complement(971..2164)
                     /locus_tag="ACFNML_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6391470.1"
                     /translation="MSDQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="ACFNML_000003"
     CDS             complement(2293..3030)
                     /gene="pxpA"
                     /locus_tag="ACFNML_000003"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFC6391471.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3017..3946)
                     /locus_tag="ACFNML_000004"
     CDS             complement(3017..3946)
                     /locus_tag="ACFNML_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HFC6391472.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDYLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="ACFNML_000005"
     CDS             complement(3943..4590)
                     /gene="pxpB"
                     /locus_tag="ACFNML_000005"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFC6391473.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4774..6981
                     /gene="uvrD"
                     /locus_tag="ACFNML_000006"
     CDS             4774..6981
                     /gene="uvrD"
                     /locus_tag="ACFNML_000006"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFC6391474.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FILDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASQEQGVSLWQAACNAGAK
                     AAKVAAFVRLIEALRNQVGQMPLPEIIAGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPDNISDDPAFPILAFLSNAALESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEEM"
     gene            7338..7877
                     /locus_tag="ACFNML_000007"
     CDS             7338..7877
                     /locus_tag="ACFNML_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6391475.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYQEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHAMFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8067..8282)
                     /locus_tag="ACFNML_000008"
     CDS             complement(8067..8282)
                     /locus_tag="ACFNML_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391476.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNSKLVQELEQQLIEAYDLMDTKG"
     gene            complement(8406..8765)
                     /locus_tag="ACFNML_000009"
     CDS             complement(8406..8765)
                     /locus_tag="ACFNML_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6391477.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8818..9194
                     /locus_tag="ACFNML_000010"
                     /pseudo
     CDS             8818..9194
                     /locus_tag="ACFNML_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            9393..10013
                     /locus_tag="ACFNML_000011"
     CDS             9393..10013
                     /locus_tag="ACFNML_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6391478.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGILYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            10036..11487
                     /locus_tag="ACFNML_000012"
     CDS             10036..11487
                     /locus_tag="ACFNML_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HFC6391479.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            11949..12305
                     /locus_tag="ACFNML_000013"
     CDS             11949..12305
                     /locus_tag="ACFNML_000013"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HFC6391480.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGVYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            12296..12778
                     /locus_tag="ACFNML_000014"
     CDS             12296..12778
                     /locus_tag="ACFNML_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HFC6391481.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            12791..13384
                     /locus_tag="ACFNML_000015"
     CDS             12791..13384
                     /locus_tag="ACFNML_000015"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HFC6391482.1"
                     /translation="MASIQNLYETVIGVLGNQAGKVISALGEITVECLPEHYISVMTA
                     LRDHEDLHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVDIYNSADWYEREAFDMYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            13374..14630
                     /gene="nuoD"
                     /locus_tag="ACFNML_000016"
     CDS             13374..14630
                     /gene="nuoD"
                     /locus_tag="ACFNML_000016"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HFC6391483.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVSPERAMQKGFTGVMLRGSGVEWDVRKTQPYEVYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            14630..15103
                     /gene="nuoE"
                     /locus_tag="ACFNML_000017"
     CDS             14630..15103
                     /gene="nuoE"
                     /locus_tag="ACFNML_000017"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFC6391484.1"
                     /translation="MLSAESLKQIDTELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGITPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQK
                     LGIGYGETTPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            15493..16794
                     /gene="nuoF"
                     /locus_tag="ACFNML_000018"
     CDS             15493..16794
                     /gene="nuoF"
                     /locus_tag="ACFNML_000018"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFC6391485.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFEAALEQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVSFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGRMEDLDLLDSVGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVHYIEHGGPMKEHKWGGW"
     gene            16794..17231
                     /locus_tag="ACFNML_000019"
     CDS             16794..17231
                     /locus_tag="ACFNML_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391486.1"
                     /translation="MVEAKIFILYGAANKGKSTTLNTLFNQICWKFSKFLVFFERHGN
                     GLDFVAVFDHEGQRIGFYSSGDNEYEVRRNLYKLYSHNCDFIFGTSRTRGGSCDAVGC
                     YAELLHGDVNIINWCEKFEPTDEDNERAVKELFKSFKNIINEL"
     gene            17521..19782
                     /gene="nuoG"
                     /locus_tag="ACFNML_000020"
     CDS             17521..19782
                     /gene="nuoG"
                     /locus_tag="ACFNML_000020"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFC6391487.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVNVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGSLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKTVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFSYEGLYHESRLKK
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGLKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELLMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAAQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     TEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTAAI
                     LKDALDAESLSSKLDNRSTWTGEGVQTASDRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            19785..20861
                     /gene="nuoH"
                     /locus_tag="ACFNML_000021"
     CDS             19785..20861
                     /gene="nuoH"
                     /locus_tag="ACFNML_000021"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFC6391488.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPKLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILIAALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            20942..21421
                     /gene="nuoI"
                     /locus_tag="ACFNML_000022"
     CDS             20942..21421
                     /gene="nuoI"
                     /locus_tag="ACFNML_000022"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFC6391489.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            21433..22104
                     /locus_tag="ACFNML_000023"
     CDS             21433..22104
                     /locus_tag="ACFNML_000023"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFC6391490.1"
                     /translation="MTFSAILFYILATIVLYGALRTVTAKNPVHAALHLVLTFCVSAM
                     LWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTDLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTINPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            22101..22406
                     /gene="nuoK"
                     /locus_tag="ACFNML_000024"
     CDS             22101..22406
                     /gene="nuoK"
                     /locus_tag="ACFNML_000024"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFC6391491.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLLMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTINVADLNKLKG"
     gene            22701..23573
                     /locus_tag="ACFNML_000025"
     CDS             22701..23573
                     /locus_tag="ACFNML_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HFC6391492.1"
                     /translation="MSFSIALPLVYLFIGILIGKTPFRKIKGTFSVILSRWIIPFVIV
                     YNITTYRDGMQYVMLWMIISMGLIILIGKRVDKDPVWNLCLCYLNIGWLGLPIVATLF
                     GDEAAQVLISAYVGSSLFGNSVGVGLLLNNSNFISGLKKTLKSPPVVALVIGMFGIPL
                     GHFVADYFGEVYFFSKIAMGILGMVILGIWLAEIKLNLNDLKKSIKPFLIKNILFIGI
                     VFLFIQIAAYFDLKLILENQKTLYLIPLLPPAANIIVLETAYLKTGRSASMIAYGTIF
                     SLIAISIYILLVQI"
     gene            23574..25598
                     /gene="nuoL"
                     /locus_tag="ACFNML_000026"
     CDS             23574..25598
                     /gene="nuoL"
                     /locus_tag="ACFNML_000026"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFC6391493.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFLNGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPITWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHDDHHGEEHHSLGKNDNPHESPLVVTLPLILLAIPSVIIGYLAI
                     EPMLYGDFFKDVIFVNTDAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQTFRPIYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSAKLVGAIAAQVRKAQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            25782..26945
                     /locus_tag="ACFNML_000027"
     CDS             25782..26945
                     /locus_tag="ACFNML_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6391494.1"
                     /translation="MTQLFLHSDTPNEKELISIYEKAVSEAVELYIFSAYLTHWDVKN
                     KLNSKLQEFKFIFGQDFGLSKKEAIRKVLKWLPSHLKFTLMVAQGIQGFHPKAVFWKN
                     DKNEYYALIGSSNLTHAAFNSNYEANILTKISEQDFIKVKSWADEIAMKSIPVSEDWL
                     EEYQEAEINYKKSTVSQSLMDKLFMEMPNYNQELIAARRKQMRNHQTVCNQLKSLIKQ
                     CAAGKIDNDEFYVEFNKLWSWKSENKGEGVGNRFQDQTWKRTGKSSDFRKLCIAIQSV
                     FDAPLAERDNVVAKQIDWLKECGVSTRGSVFSEMLCQEYPDRYPVLNAPIKKFLEKNK
                     FKSAKGASEGSRYIDLSMKLRALLAMQSEIKNLAELDVLVQAEYRDSTDIDWE"
     gene            27055..28551
                     /locus_tag="ACFNML_000028"
     CDS             27055..28551
                     /locus_tag="ACFNML_000028"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFC6391495.1"
                     /translation="MFSNYLLSLAIWIPIAAGVLVLSTGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFVAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AVIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGEIRNPHVADM
                     KDINCREFAVLAVLAAAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKI"
     gene            28561..30006
                     /gene="nuoN"
                     /locus_tag="ACFNML_000029"
     CDS             28561..30006
                     /gene="nuoN"
                     /locus_tag="ACFNML_000029"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFC6391496.1"
                     /translation="MNWSDLNLMPAMPEIVLLSLLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYARPYNQVRG
                     IFKGEFYTLSLFALLGMGVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVG
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKVAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVVMSLIGAFYYLRVVKVMYFDESGRARLAAGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            30118..30417
                     /locus_tag="ACFNML_000030"
     CDS             30118..30417
                     /locus_tag="ACFNML_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HFC6391497.1"
                     /translation="MTASMYILLVLALIFANAPFLTTRLFGVAALKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHNQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(30526..31422)
                     /locus_tag="ACFNML_000031"
     CDS             complement(30526..31422)
                     /locus_tag="ACFNML_000031"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HFC6391498.1"
                     /translation="MNPTNDLKAWQLRAQAQTELLLERFLPSEGEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGAVADAVGQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHIQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGAMSCPNLSEDSLAVLDDYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(31406..31630)
                     /locus_tag="ACFNML_000032"
     CDS             complement(31406..31630)
                     /locus_tag="ACFNML_000032"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009115303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFC6391499.1"
                     /translation="MKKNAPKSFEEALSRLESLTQSMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            complement(31877..32800)
                     /gene="rsgA"
                     /locus_tag="ACFNML_000033"
     CDS             complement(31877..32800)
                     /gene="rsgA"
                     /locus_tag="ACFNML_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HFC6391500.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIEAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAQTGAASPERLAFLQGITDELPG"
     gene            complement(32805..34661)
                     /locus_tag="ACFNML_000034"
     CDS             complement(32805..34661)
                     /locus_tag="ACFNML_000034"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6391501.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMAGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVVWAY
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVSQGEIKFEHIDFCYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGVTQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(34687..34824)
                     /locus_tag="ACFNML_000035"
     CDS             complement(34687..34824)
                     /locus_tag="ACFNML_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391502.1"
                     /translation="MPSEGGAAFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(34824..35408)
                     /gene="ruvA"
                     /locus_tag="ACFNML_000036"
     CDS             complement(34824..35408)
                     /gene="ruvA"
                     /locus_tag="ACFNML_000036"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFC6391503.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVGGIGAKTALGILSAMTADELARAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHTVTDGLFAAAPAADETEDIVSTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(35490..35816)
                     /locus_tag="ACFNML_000037"
     CDS             complement(35490..35816)
                     /locus_tag="ACFNML_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6391504.1"
                     /translation="MNIKHLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(35878..36597)
                     /locus_tag="ACFNML_000038"
     CDS             complement(35878..36597)
                     /locus_tag="ACFNML_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HFC6391505.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGQSALV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNLASSASNPNLSVEKRHYEYVVKMGPFSSQ
                     ERAAEAEAQARGMVRAVLTAG"
     gene            complement(36594..36716)
                     /locus_tag="ACFNML_000039"
     CDS             complement(36594..36716)
                     /locus_tag="ACFNML_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6391506.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(36727..37191)
                     /gene="trmL"
                     /locus_tag="ACFNML_000040"
     CDS             complement(36727..37191)
                     /gene="trmL"
                     /locus_tag="ACFNML_000040"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFC6391507.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVHENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(37256..37981)
                     /locus_tag="ACFNML_000041"
     CDS             complement(37256..37981)
                     /locus_tag="ACFNML_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HFC6391508.1"
                     /translation="MDFLSCWRRIADAPTIRRCVLCHGSSGVSDGICAGCRDDLAAYR
                     TDAANSCPLCFMHVQGGAVCGGCQKKPPAFDRMWASLHYEPPVSNMIHALKHLADLSM
                     VQPLADLMMQNPPDRLADECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQIL
                     PRHTVFRHHRPPQSTLKGGERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELA
                     KTLKKSGANRICCWTLARTPMKK"
     gene            37980..38723
                     /gene="bioH"
                     /locus_tag="ACFNML_000042"
     CDS             37980..38723
                     /gene="bioH"
                     /locus_tag="ACFNML_000042"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFC6391509.1"
                     /translation="MPDAVKKVYLIHGWGANRHVFDDLMPRLPATWPVSAVDLPGHGD
                     APFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFAR
                     LTADEDYPEGLAAPALGKMVGAFRTDYAKHIKQFLQLQLLHTPDADGIIGRILPDLAR
                     CGTPSALQEALDAAERADARHLLDKIDVPVLLVFGSKDAITPPRMGEYLHRRLKGSRL
                     VVMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            38725..39513
                     /locus_tag="ACFNML_000043"
     CDS             38725..39513
                     /locus_tag="ACFNML_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6391510.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWARALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGVWRAMAWNDENAA
                     RSCVGTIFEREGGLSITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            39651..39920
                     /locus_tag="ACFNML_000044"
     CDS             39651..39920
                     /locus_tag="ACFNML_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391511.1"
                     /translation="MYCLRLRRLVLIFVNPLYADAGDVYCVYNIRLKQYTACQVTRVE
                     EHGGKKTHATLLALDWQGNKPLGAEELADLKPLYKDLKNNIGNIV"
     gene            40296..>40643
                     /locus_tag="ACFNML_000045"
     CDS             40296..>40643
                     /locus_tag="ACFNML_000045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HFC6391512.1"
                     /translation="MPTGYVRVGNTAPLVGEDTQRYASFWGDGYDVYRQLRWQQIPEK
                     QRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELKAFPCLSSLFLTKWHK
                     NLYDSGLTKTSTAL"
BASE COUNT         9361 a        10201 c        10817 g        10264 t
ORIGIN      
        1 tttcagttac agcagcttga tccctttccc ttatccaacg ggggaaggct aggatagggt
       61 ggcttgcaaa tatacagaac aagggacaag agccaccctc tctccaaccc tctccctccg
      121 tacgggaggg ggtggattct cgcgggcgaa gcccacgcta cggttagcct ttaccccagc
      181 acaaacaatt cccgcccgtg cgccttcagc caacttttag cattgtcggt atgcggcgtc
      241 agcgtgttca ccaaatgcca aaagcgcgga ctgtggtcgg ggtggcggag gtggcagagt
      301 tcgtggatgc agacatagtc ggcaacgtac ttgggcgcgc cgatcagccg ccagttgagg
      361 cggatgccgg tgtgcgggcg gcatacgccc caaaaggttt tggcgttgct caggtctgtg
      421 gcggtgggcg tcagtcctgt ttcggctgcg tgtttttcaa ggcggggcag caggtattcg
      481 cgggcgcgtt cgttcaacag gcggcgcagg tggtcgattt gtgcggcggt ttcttttcgg
      541 ggaagcagga tttcagacga cgtgatacgg atatggcttt ggctgtgggt atccagcttg
      601 gtctttattc cccgatacca aatccactcg ggtaagtttg ggtgggaaac aggatgcacg
      661 ggcgttttgg caagcgtgtt ccgcaaaatc gtttcgtttg ccgccagcca gtttgctaac
      721 gcgtggtctt gaaaaaaggg tgggacgttg atgctgaccg tctgcatatt gacggggcgc
      781 agaatcagat ttttcttggc actgcgtttg agttcgattt cgatgcacaa gccgtcggaa
      841 agagtatagg tgaagcgttt catagttgtg aataggtttc agaccggata catcgtctga
      901 aacaggaatt ttccatatca ggcggcaaac ttcggataat atacaaaatc aaacatctgc
      961 gctacaaggt tcagccgaac aagccgccga tatatttgct gatggtgatg gcgctgagta
     1021 ctgccatcaa accgaccaca atcacgccgg aaacggtgag ccacagcggg tgtttgtagt
     1081 cgccgacaat tttggttttg taggcggcaa tcagaatcag accgagggaa atcggtaaaa
     1141 tcaggccgtt taatgcgcct acgaacacca gcacctgcgc cggtttgccg atggtggaaa
     1201 atacggcggt ggacacggcg ataaaggcaa taatccattt gtttttattg cgttcgatag
     1261 acgggctgag accggagaag aacgacaccg aagtataagc cgcaccaatc accgaagtaa
     1321 tcgaagccgc ccaaatcacc acgccgaaaa tcagcaggcc gatgtatccc gccgcatatt
     1381 caaacggtgt ggaagcaggg ttgtcgggat tgagctgtac gccttggctg accacgccca
     1441 aaaccgccaa aaacaataca atccgcataa tcgaggcaat caggatcgcc cgcaccgagc
     1501 tttggctcac ttccggcaac gccgatttgc ctttgatgcc tgcgtccagc agacggtgcg
     1561 caccggcgaa ggtgatgtag ccgccgaccg tgccgcccac cagtgtaaca atcgccattg
     1621 catcgagttt ttccggcata aaggtatgca cggcggcatc tgccagcggc ggattcgcct
     1681 gccatgccac ataaaccgtc agcgcaatca ttacgaaacc catcacttgg gcgaatttgt
     1741 ccatcacttt gcctgcttct ttaaacagaa acacaccgat ggcaatcacg ccgctgatca
     1801 cggcaccggt ttccggcgac agtccggtca gcaggttcag acccaagcct gcgccgccga
     1861 cgttgccaat attgaacgcc aaaccgccca tcacaatcag cacagccaag aaatagcctg
     1921 cgccgggcaa gacctgattg gcaatatcct gcgcctgttt ttcggaaacg gcgacaatcc
     1981 gccaaatatt gagctgcgcc ccgatgtcga gcagaatcga gagcagaatc acaaagccga
     2041 aacttgccgc cagtgcttgg gtgaaggtgg cggtttgggt cagaaagccc gggccgatgg
     2101 cggaagtcgc catcaggaat gcagcgccga ttaaggcatt tctgcggttt ttttgatcag
     2161 acataatcgc ttatcctcta taaaattggt tgttgctgtg tttgggcgaa acctgcggtt
     2221 ttagctacgc agaaactcgc tttgctcgtt ttggcgaaac ctgcggtttt cagacggcct
     2281 atgaactgtt tttcaagcag aaactttgat gcctgccgcc agtagttcct gccggatttt
     2341 ttcggcaaac accacggcgt gcggcccgtc tccgtgcaga cagatgctgt cggcttgcac
     2401 ggcaaccagg ctgccgttca ctgctttgac ccgcccgtcc cgcaccatct gcaatacttg
     2461 ggcgatggct tcttcgtcgc tgtcaacctg cgcatcgggg cggctgcggg gaaccagcgt
     2521 accgtcgggc atatagcggc ggtcggcgaa tacttcggaa atcacaccca agcctgcggc
     2581 ttttccggct tccaagagca ggctgccgga aagtgccatc aatttcaatt tcgggtcgaa
     2641 atccgccaca attcgggcaa cggtatccgc cagcgcacgg tttttcgccg cttggttgta
     2701 cattgcgccg tgcggtttga cataagccat ttccaaaccc tgatcacggc acaaggcctg
     2761 caatgcgccc aactggtaat tcagacacgc ccgcaaatcg gcttcggaca gattcatttc
     2821 ggtacggccg aagttttccc gatcgggata gccggggtgt gctccgatgc gcacgccgtt
     2881 ttgttgggca tacgccaatg ccgcccgaat atcggcaatg ctgccggcgt gttgggcgca
     2941 ggcgatgttg gccgaagtaa tcagctgcaa caaggcttcg tcgctgccgc agccttcggc
     3001 gagatcggcg tttaaatcaa cctgcttcat gggtgattct ccgtatttgg ttcagatagg
     3061 cttggttttt gcgccgcagg gcggtggctt ctttcaagcc gattattttg aatttgactt
     3121 tgctgccgaa gcgcacctgt gccagcctgc ccaaatcggc ggcggcaacg gtagcgattt
     3181 tcggataacc gccggtggtt tgtgcatcgg ccagcaggat aatcggtttg ccgccgggcg
     3241 gcacctgcac ggttcctgcc tgaacagcgt gggacagcat ttccaaaggt tgcgacaggg
     3301 tcagcggctg tccgtcgaag cggtagccca tgcggttgct atcgctttgc agcgtccacg
     3361 tttcccgttc cagattcaga cgcccttttt cactgaaagc ggcatattcc gacgaaggaa
     3421 caaggtggac ggtatcggta aacggtatcg gggcaatgcc gactttggac aattcctgcg
     3481 cacctttgcc gatggggaga taatcgcctt tttgcagcat tctgccctga tggccgccga
     3541 aaccggcttt caggtcggta cttctcgaac ccatcacttc cggcacatca aatccgcccg
     3601 ccacgcacac atagccgtac atgccctgca cggcacgcac cattttcaag gtctgccctt
     3661 tgcgggcggt ataacgccaa tacgaataga ccggttcgcc gtccaattcc gcctgataca
     3721 cggcaccggt gagacaaaac ggcgtatccc gttcaaacac cagcattatc ccgcccaaag
     3781 cgatttcgat tgcggccgtg ccttcgtcgt tgcccaataa aatattaccc gccgccaaag
     3841 caaccgtgtc catcgcaccg gcatgaccga tgccgtaacg ccggtgtccg tagcgtccgg
     3901 tatcctgaat atgcgccggt gcctgcactg ccgaaacgtg aatcatggct caatcctttc
     3961 tgcaacaaag cggacttggt cacccgccgc cagcagggtc ggcggattca aatcggctcg
     4021 gaacaagggt aattcggttc tgccgataat ctgccagccg ccgggcgaag cgaacggata
     4081 cacaccggtc tgactgccgc cgataccgac cgaaccggca ggaacggacg ttctcggcac
     4141 ggcacggcgg ggcgtgtgca atgcttcggg caagccgccc agataaggga aaccgggctg
     4201 gaagcccatc ataaatacgg tataagtttg cgccgtatgg cggcggacga tttcggaaat
     4261 aaccgtctga tggaaagcag cgacttccgc caaatccggg ccgtattcgc cgccgtagca
     4321 gacgggaatt tccaccagtt tgccctgatg gtctgtaacg gcggtgtgtt cccacacata
     4381 ttgcaattca tcggcaagcg tcgccaaatc ggtatcgaaa cgggtaaaca cggtcagatt
     4441 gttcatgccg accaccactt cctcaatcct gtcgtgctgc ccgagcgcag cggcaaacgc
     4501 ccacaacttt tgctgtttgc ccagttcgga aggcgcattc agtcggcaga ccaaagcgga
     4561 ttcgctgatt ggtgtgatct ctattctcat ttgttgttca ttttggttat gttttaatga
     4621 atctatatgc aggggcggcg gtttgtcaat atcttctgtg ctgcatcatc aaaccgtcga
     4681 ttggaaaagt gctgccctgc cgctgcactt tttcagacga ccttaaaccg tttctattaa
     4741 aatagcgcat tccacttttc agacggcatc cttatgtttc ccgaccaatc cgcccccaac
     4801 ctgctgcaag gcttgaatcc cgaacaactc tccgccgtaa cctggccgcc gcaatccgca
     4861 cttgtgctgg cgggcgcggg cagcggcaaa acgcgcgtgc tgaccacgcg catcgcatgg
     4921 ctgttgcaaa ccggtcaggc aagcgtgcac agcattatgg cggtaacgtt taccaacaaa
     4981 gccgccaaag aaatgcaaac ccgtttgggc gcgatgattc ccatcaatgt ccgcgccatg
     5041 tggctcggca cgttccacgg cctctgccac cgctttttgc gcctgcacca ccgcgacgcc
     5101 ggtctgccgt cttcctttca aatcctcgac ggcggcgacc agctttccct catcaaacgc
     5161 ctgctcaaaa gcctcaacat cgccgaagaa atcatcgcgc cgcgttcgct gcaaggcttt
     5221 atcaacgcgc aaaaagaatc cggtttgcgc gcttccgtct tgagtgcgcc cgatccgcac
     5281 acaagccgca tgattgaatg ctacgccgaa tacgacaaaa tctgccaacg cgaaggcgtg
     5341 gtcgattttg ccgaactcat gctccgcagc tacgaaatgc tgcaaagcaa cgaaatcctg
     5401 cgccagcatt accaaaaccg cttcaaccac attctcgtcg acgagttcca agacaccaac
     5461 aaactgcaat acgcctggct caaactcatg gcgggcggaa acgcagcggt atttgccgtc
     5521 ggcgacgacg accaaagcat ttaccgtttc cgtggcgcaa acgtcggcaa catgaccgcg
     5581 ctgatggaag aattccacat cgacgcgccc gtcaaactcg aacaaaacta ccgctccgtc
     5641 ggcaacatcc ttgccgccgc caatgccgtg attgaaaaca acgacgaacg actcggcaaa
     5701 aacctgcgca ccgacgccga agcaggcgac aaaatccgct actactccgc ctttaccgac
     5761 ctcgaagaag cccggttcat cttggacgaa accaaagccc tcgaacgcga aggctgggat
     5821 ttggacgaaa tcgccgtcct ctaccgtagc aacgcccaat cccgcgttat cgaacaaagc
     5881 ctgttccgca gcggcatccc ctacaaaatc tacggcggct tgcgctttta cgaacgccaa
     5941 gaaatcaaac acgcgcttgc ctatctgcgc ctcgccgtca atcccgacga cgacaacgcc
     6001 ctcttgcgcg tgattaactt cccaccgcgc ggcatcggcg cacgcaccgt cgaaaacctt
     6061 cagacggcat cgcaagagca aggcgtttca ctttggcagg cagcctgcaa tgccggcgca
     6121 aaagccgcca aagtcgccgc cttcgtccgc ctcattgaag ccctgcgcaa ccaagtcgga
     6181 caaatgcccc tgcccgaaat catcgccggc atcctcaaag acagtggctt gaccgaacac
     6241 taccgcaccc aaaaaggcga caaccaagac cgcctcgaca accttgacga actcgtcaac
     6301 gccgccatcg agttcaaacc cgaagacagc aactttgaaa ccctgcccga caacatttca
     6361 gacgaccccg ccttccccat cctcgctttc ttgagcaacg ccgccctcga atccggcgaa
     6421 aaccaagcag gcgcaggcga aaaagccgtc caactgatga ccgtccacgc cgccaaaggc
     6481 ttggaattca acgccgtctt cctcaccggc atggaagaag gccgcttccc cagcgaaatg
     6541 agccttgccg aacgcggcgg cctcgaagaa gaacgccgcc tcatgtacgt cgccatcacc
     6601 cgcgcccgca aacgcctcta catcaccatg gcgcaacaac gcatgctgca cggacaaacc
     6661 caattcggca tcgtctcccg cttcgtcgaa gagatcccac ccgaagtatt gcactacctg
     6721 tccgtcaaaa agaccgccta cgacagctac ggcagcccgc gccaaaccgc cgcatccaaa
     6781 gacaaaatca tcgacgacta caaacagccc caaacctacg caggtttccg tatcggacaa
     6841 aacgtccgcc acgccaaatt tggcaccggc gtgattatcg atgccgcaga taaaggcgaa
     6901 tccgcccgac tgaccatcaa tttcggcaaa cagggcgtga aagagttgga caccaagttt
     6961 gcgaaattgg aagagatgta aatttgaaat gcaggctgga tactcgtatc cgacaaaaat
     7021 atttgacgcg tctatcgttt ccgaaaaacc gctgttggaa aatgtcggat tcgagaatcc
     7081 gacctatggc aaaaaactgt agcaaggaca aggcaaaagg ccgtctgaat ccggtaacaa
     7141 caaattttgc cgaaacctcc gccgtcattc ccgcgcaggc gggaatccag acctttcggc
     7201 acggaaactt atcggataaa aggtttcttt agattccacg ttctagattc ccgcctgcgc
     7261 gggaatgacg gacggtaaaa gccgggtata gatacctaac ccttgtttat agctgaaaat
     7321 caataaggaa gaacattatg tcccaagttt ttaaagattt tgatttgtcc tccgtatggg
     7381 aatccgacag ttgggcagat gaaaactacc aagaagcccc gtttacccct gaaattttgg
     7441 ctgccgtaga aagtgaactg ggctataaat tgccgcaaag ttttattgaa ttgatggcgg
     7501 tacaaaacgg cggaatattt gtcaaaaact gttttccgac cacgcagaga aattcgtggg
     7561 cggaaaatca tgtgcaaatt tgcgaggtat cgggcatcgg ctttgaaaaa gaagggagtt
     7621 tgtgcggcga aacagggcaa aaactttggc tggaagaatg ggaatatccg cccatcggcg
     7681 tgtattttgc caacgacccg tcaggcggtc atgccatgtt tgccttggac tatcgggcgt
     7741 gcggcaaaga cggcgagccg aaagtggtgt ttgtcgagca agaatcggat tttgaaatcg
     7801 tcgaacttgc ccccgatttt gaaaccttta tccgcagctt gcggcatgaa gatgagttta
     7861 ttgacgagga aatataaaac ggcggttgaa aaaccggaat catcaaggga aaacgggcga
     7921 aataacggat aatcgcttga atccgtaagg aaaacggttt ggtggaacgc gccatccaag
     7981 acctttgcaa agaaaaactg tctccgacag cattgacatt attaacagaa cttatcaagt
     8041 ttggagctat ctcaaatata attcggttat ccttttgtat ccattaaatc atatgcttca
     8101 attaattgtt gttctagctc ttgtaccaat tttgaattgc gaattcctga cacaatctca
     8161 aattcttctg catctatgca aacacctgca taaatttcaa taacaaggga acgcaataat
     8221 tgaagctctt ctcttgttaa agaaataata atgtcatcac ctgtgtaatt gattatattc
     8281 ataataattt tatttttgtt tgtcaaagta agttttgcct aaggttggtc taaatgcagt
     8341 tccaccatct tttgaatttg ggtctctgat tacaattgct ccagacttat catcccaaat
     8401 tgctcttatg tgtttggatt gtaatcttcg aattcccaag aaaaaaatcg taataagttt
     8461 gaaagtgtca aatcccaagt ttcttttgag caatattcta atattttatc aatttcactt
     8521 ttaataatct tatgatcaaa ctgttctaat attaatgcat tagaccaaaa aaaaccttct
     8581 ttattacaat gatgggaaat ccatttagga gaacaaatgc aaagtgaaaa aatagatgag
     8641 ccttgttctc cttcgattcc gatatccaaa tctatccatc tatggaaatt atctggaatt
     8701 tcgggggtaa atttttcaaa atcaatatca tataaattta tgctttttaa atccaattta
     8761 atcattaggg ctgtcctaga taaataggga aattcaaatt aagttagaat tatccctatg
     8821 agaaaaagtc gtctaagccg gtataaacaa aataaactca ttgagctatt tgtcgcaggt
     8881 gtaactgcaa gaacagcaac agagcctgac agcattgttt atacggattg ttatcgtagc
     8941 tatgatgtat tagatgtgag cgaatttagc catttccgta tcaatcacag cacacatttt
     9001 gctgaacgac aaaaccatat caatggaatt gagaattttt ggaaccgggc aaaacgtcat
     9061 ttacgcaagt ttaacggcat tcccaaagcg cattttgagc tgtatttaaa ggagtgcgaa
     9121 tggcgtttta acaacagtga gataaaagtt cttgttccat tttaaaacaa ttagtaaaat
     9181 cgagtttatc ttagttgtcc aggacagccc cattattttt ataacaccgt gaagccgcac
     9241 agcagtttga acagtgatac gccgtttgcg ggcttacgag tttattttcc cggcctgcag
     9301 tttgagcaat acggtgattt cctacggtta atacaaatgt ttacacattg atacatttca
     9361 tttatagttc cgcctatttg aaaatagaaa atatgaattc gaccgcaagt aaaaccctga
     9421 aaggattgtc gctggtgttt ttcgcctctg gattctgcgc cctgatttac caggtcagct
     9481 ggcagaggct tctattcagt cacataggta tcgatttaag ttcgattact gtcattattt
     9541 ctgtatttat ggtcggcttg ggtgtgggtg cgtatttcgg tggacgcatt gctgaccgtt
     9601 ttccttcaag tatcatcccc ctgttttgca tcgctgaagt atccatcggt ctgttcggtt
     9661 tggtaagcag gggtctgatt tccggcttgg ggcatctttt agttgaggct gatttgccca
     9721 tcatcgctgc tgccaatttc ctcttattgc tgcttcctac ctttatgatg ggcgcgacct
     9781 tgcccttgct gacctgtttt tttaaccgga aaatacataa tgttggcgag tctatcggta
     9841 tcttatattt tttcaacact ttgggtgcgg cactcggatc gcttgccgcc gccgaatttt
     9901 tctacgtctt ttttaccctc tcccaaacca ttgcgctgac agcctgcttt aaccttctga
     9961 ttgctgcttc agtatggctg cgttacagaa aggatggata tagtgaacac taaaccgaat
    10021 actagtttga tttatatgct ttctttcctt agcggcttat tgagcttggg tatagaagtc
    10081 ttgtgggtaa ggatgttttc gttcgcagca cagtccgtgc ctcaggcatt ttcatttact
    10141 cttgcctgtt ttctgaccgg tatcgccgtc ggcgcgtatt ttggcaaacg gatttgccgc
    10201 agccgctttg ttgatattcc ctttatcggg cagtgcttct tgtgggcggg tattgccgac
    10261 tttttgattt tgggtgctgc gtggttgttg acgggttttt ccggcttcgt ccaccacgcc
    10321 ggtatcttca ttaccctgtc tgccgtcgtc agagggttga ttttcccgct cgtacaccat
    10381 gtgggtacgg atggcaacaa atccggacga caggtttcca atgtttattt cgccaacgtt
    10441 gccggcagtg cattgggtcc ggtccttatc ggctttgtga tacttgattt cttgtccacc
    10501 caacagattt acctgctcat ctgtttgatt tctgctgctg tccctttgtt ttgtacactg
    10561 ttccaaaaaa gtctccgact gaatgcagtg tcggtagcag tttccctaat gttcggcatc
    10621 ctcatgttcc tactgccgga ttctgtcttt caaaatattg ctgaccgtcc ggataggctg
    10681 attgaaaaca aacacggcat tgttgcggtt taccatagag atggtgataa ggttgtttat
    10741 ggggcgaatg tatacgacgg cgcatacaat accgatgtat tcaatagtgt caacggcatc
    10801 gaacgtgcct atctgctacc ctccctgaag tctggcatac gccgcatttt cgtcgttgga
    10861 ttgagtacag gttcgtgggc gcgcgtcttg tctgccattc cggaaatgca gtcgatgatc
    10921 gttgcggaaa tcaatccggc ataccgtagc cttatcgcag acgagccgca aatcgccccg
    10981 cttttgcagg acaaacgtgt tgaaattgta ttggatgacg gtaggaaatg gctgcgtcgc
    11041 catcctgatg aaaaattcga cctgattttg atgaatacga cttggtactg gcgtgcctat
    11101 tccaccaacc tgttgagtgc ggaattttta aaacaggtgc aaagccacct taccccggat
    11161 ggtattgtaa tgtttaatac cacgcacagc ccgcatgctt ttgctaccgc cgtacacagt
    11221 attccctatg cataccgcta tgggcatatg gtagtcggct cggcaacccc ggtagttttc
    11281 cctaataaag aactgctcaa gcaacgtctc tcccggttga tttggccgga aagcggcagg
    11341 cacgtatttg acagcagcac cgtggatgct gcagcacaaa aggttgtctc tcgtatgctg
    11401 attcagatga cggaaccttc ggctggggcg gaagtcatta ccgacgataa tatgattgta
    11461 gaatacaaat acggcagagg gatttaaccg tcttaaaggg tttcaggcaa cgcaggtttt
    11521 aggtaacgtc ctgctagttc aaaaaaaccg catcacagca gtcgggacaa aatggtttaa
    11581 acattttgtc ccgaattctt attcctatat atagtggatt aacaaaaatc aggacaaggc
    11641 gacgaagccg cagacagtac agatagtacg gaaccgattc acttggtgct tgagcacctt
    11701 agagaatcgt tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaatccac
    11761 tataccacga attacggtgt aaaaatttat atgaccttat aaaatcaaat aagaatcgtt
    11821 atcataacat gattgtattt attgggtttt tttgggcgtt ttgccgatat ttacctttta
    11881 atggtttttg aaattcgcta aaatacgaaa ttattgtaga aattttgtta acggatttgg
    11941 gtgtaaccat gttgtccgct tactttcccg tctttgtctt tatccttgtc ggtcttgcag
    12001 ccggcgtact gtttatcctg ctcggcacaa ttttgggccc gaaacgtcac tatgccgaaa
    12061 aagacgcgcc ttacgaatgc ggttttgaag cctttgaaaa cgcaaggatg aagttcgacg
    12121 tgcgctatta cctcgtcgcc atcctcttca tcctgtttga tttggaggtc gcgtttatgc
    12181 tgccgtgggc tgtcgtgttc aaagatttgg gcgtgtacgg cttctggtct atgctggtgt
    12241 ttatcgtcgt tctgacggta ggctttgttt acgaatggaa aaaaggtgcg ctggaatggg
    12301 aatagaaggc gttttgaaaa aaggtttcat caccaccagc gcggatacgg tgctgaacta
    12361 tatgcgtacc ggttcattgt ggccggttac tttcggcttg gcctgctgcg ccgtggaaat
    12421 gatgcatgca ggtatggcgc gttatgacct tgaccgtttc ggcatcattt tccgtccgtc
    12481 cccacgtcag gccgacctga tgattgtggc aggtacgctg accaacaaaa tggcgcccgc
    12541 cctgcgccgc gtatacgacc agctcgccga gccgcgttgg gtattgtcta tgggctcgtg
    12601 tgccaacggc ggcggttatt atcactattc ttattccgtt gtgcgcggtg ccgaccgcgt
    12661 cgtgccggta gacgtttatg tgccgggttg tccgccgact gcggaagccc tgatttacgg
    12721 cctgattcag ctccaacaaa aaatcaagcg cacttccacc atcgcgcgtg acgagtaagg
    12781 agaggacgat atggcaagca ttcaaaattt atacgagacc gtcatcggtg tgctgggcaa
    12841 tcaggcaggc aaagtcattt cagctttggg cgagattacc gtcgagtgtc tgcccgaaca
    12901 ctatatctca gtcatgaccg cattgcgcga ccatgaagat ttgcatttcg agcttctggt
    12961 cgatttgtgc ggtgtcgatt acagcactta caaaaacgaa gtatggcagg gcaaacgctt
    13021 tgccgtcgtc agccagctgc tttccgttaa aaacaatcaa cgcatccgcg tacgcgtctg
    13081 ggtttcagac gacgacttcc ccgtagtcga atccgtagtc gatatttaca acagcgcgga
    13141 ttggtacgaa cgcgaagcct tcgatatgta cggcatcatg ttcaacaacc acccggactt
    13201 gcgccgcatc ctgaccgatt acggctttgt cggacatccg ttccgcaaag acttcccgat
    13261 ttccggctat gtggaaatgc gttacgacga agagcaaaaa cgcgtgattt accaacctgt
    13321 taccattgag ccgcgcgaga tcacgccgcg tatcgtccgt gaggagaact acggtggcca
    13381 ataaattaag aaactacacc atcaacttcg gcccgcaaca ccctgcggcg cacggcgtat
    13441 tgcgtatgat tttggagttg gagggcgaaa ccatcgtccg tgccgatccg catatcggcc
    13501 tcctgcaccg cggtactgaa aaactggctg aaaccaaaac ctatctgcaa gccctgccct
    13561 atatggaccg cttggactac gtttccatga tggtcaatga gcaggcgtat tgtttggcag
    13621 tagaaaaact tgccggtatc gatgtaccta tccgcgccca gtatatccgt gtgatgtttg
    13681 ccgaagtaac gcgcatcctc aatcacttga tgggcatcgg ttcgcatgcc ttcgacatcg
    13741 gcgcgatgac cgccatcctc tacgccttcc gcgaccgcga agagctgatg gacttgtacg
    13801 aagccgtttc cggcgcgcgt atgcacgcag cctacttccg tcccggcggc gtttaccgcg
    13861 acctgcccga cttcatgcct aaatacgaga gcagcaaatt ccgcaatgcc aaagtattga
    13921 agcagctcaa cgaatcccgc gaaggcacca tgctcgactt tatcgatgcc ttctgcgaac
    13981 gcttccccaa aaatatcgac acactcgaaa ccctcctgac cgacaaccgc atctggaaac
    14041 agcgtaccgt cggcatcggc gtcgtctccc ccgaacgcgc catgcaaaaa ggctttaccg
    14101 gcgtgatgtt gcgcggttcg ggcgtggaat gggacgtgcg taagacacag ccttacgaag
    14161 tgtacgacaa aatggatttc gacatccccg tcggcgtcaa cggcgactgt tacgaccgtt
    14221 acctctgccg tatggaagaa atgcgtcaat ccgtacgcat catcaaacaa tgttccgagt
    14281 ggttgcgtgt caatccgggt ccggtcatta ccacaaacca caaatttgct ccgcccaaac
    14341 gtaccgaaat gaaaacaggt atggaagacc tgattcacca tttcaaactc tttaccgagg
    14401 gtatgcacgt tcccgagggt gagacctaca ccgctgtcga acatccgaaa ggcgagttcg
    14461 gcgtttacat catttcagac ggcgcaaaca aaccctaccg cctgaaaatc cgcgcacccg
    14521 gcttcgccca cttgcaaggc atggacgaaa tggcaaaagg ccacatgctc gccgacgtcg
    14581 ttgccatcat cggtacgcag gacatcgtat tcggggaggt tgaccgataa tgttatccgc
    14641 agagtcctta aaacaaatcg acacagaact ggcgaaatat cccgccgacc aacgccgttc
    14701 cgccatcatg ggtgcgttgc gtatcgccca aaccgaaaaa ggctggctcg cccccgaaac
    14761 catcgctttt gttgccgact atatcggcat cacgcctgca caagcctacg aagtcgccac
    14821 tttctacaat atgtacgacc ttgagcctgt cggcaaatac aaactgaccg tttgtaccaa
    14881 cctgccctgc gccctgcgcg gcggtatggc taccggcgaa tacctcaaac aaaaactcgg
    14941 tatcggctac ggcgaaacta cccctgacgg caagtttacc cttgtcgaag gcgaatgcat
    15001 gggcgcatgc ggcgacgctc ccgttatgct ggtcaacaac cacagcatgt gcagctttat
    15061 gaccgaagaa gcgattgaga agaaactggc ggagttggag taggtcgtct gaaacgacga
    15121 tttaaacgta ggtcggatac ttgtagccga cagagtgggt aaaaaggcaa aatgtcggat
    15181 ttaagaatcc gccctactga aataccgaaa tgccgtcatt cccgcgcagg cgggaatcca
    15241 ccggtaagat tcggtttctg aatttaataa gacattgctt accattgagg atggattccc
    15301 gcctgcgcgg gaatgacgac agacaagcaa gtggtcgaga tccaacaaaa acgattaaag
    15361 gtcgtctgaa aatatcgatt tgataaacta gattttattt cagacgacgt tacaagccgg
    15421 tacacaaaga catcttaagg tcgtctgaaa cagcggccgc aaccgatacg aaaacaaaca
    15481 ggcacaccaa aaatggctat ttaccaatca ggcgtgattt ttgaccaagt ggataccgcc
    15541 aatcccgatt gctggacatt ggacgaatac gtcaaacgtg gcggttatac cgccctgcgt
    15601 aaaatcctgt ccgaaaacat ctcgcaaacc gatgtgattg acgaagtcaa aacctccggc
    15661 ttgcgcggtc gtggcggtgc gggcttcccg accggtttga aatggagctt tatgccccgt
    15721 tctttccccg gcgaaaagta cgtcgtttgc aacaccgacg aaggcgagcc gggtacattt
    15781 aaagaccgcg acattattat gttcaacccc catgccctga ttgaaggcat gattatcgcc
    15841 ggttacgcga tgggcgcgaa agccggttac aactatatcc acggcgaaat ttttgaaggc
    15901 taccaacgct ttgaggccgc tttggagcag gcgcgtgccg caggcttttt gggtaaaaat
    15961 attttgggtt cggattttga attcgaactc ttcgcccacc acggctacgg tgcatacatc
    16021 tgcggcgaag aaaccgcatt gcttgaatcg ctggaaggca aaaaaggcca gccgcgcttt
    16081 aaaccgccat tccctgcttc gttcggcctg tacggcaaac cgactaccat caacaatacc
    16141 gaaactttct cctccgtttc attcattatc cgtgacggcg gacaggcatt tgccgataaa
    16201 ggtattccga atgcaggcgg taccaaatta ttctgtattt ccggccatgt cgagcgtccg
    16261 ggcaactatg aagtgccatt gggcacaccg tttgccgaag tcttgaaaat ggcaggcggt
    16321 atgcgcggcg gcaaaaaact caaggccgtt attcctggcg gttcgtccgc gcccgtattg
    16381 cctgccgaca tcatgatgca gacaaatatg gactacgact cgatttccaa agcaggctcg
    16441 atgctgggtt ctggcgcgat tatcgtcatg gacgaagacg tgtgcatggt gaaagccctt
    16501 gagcgcctga gctacttcta ctacgacgag tcttgcggcc aatgtacgcc ttgccgagaa
    16561 ggcaccggct ggctttaccg catcgtccac cgcatcgtag aaggcaaagg ccgcatggaa
    16621 gatttggatt tgctggattc tgtcggcaac caaatggcag gccgcaccat ctgcgccctt
    16681 gccgatgccg ccgtcttccc cgtccgaagc tttaccaagc atttccgtga tgagtttgtg
    16741 cattacatcg aacacggcgg accgatgaaa gagcataagt ggggagggtg gtaatggtgg
    16801 aagctaaaat ttttattcta tacggtgcag ccaacaaagg taagagtacg acactcaata
    16861 cgctttttaa tcagatttgt tggaaatttt ctaaatttct agtctttttt gaaagacatg
    16921 gaaatggctt agattttgtt gcagtatttg atcatgaagg tcagagaatt ggtttttatt
    16981 catctggtga taatgaatac gaggttagga gaaatttata caaactttat tcgcataatt
    17041 gtgattttat ttttggcacg tcaaggacac ggggtggtag ttgcgatgca gtaggatgtt
    17101 atgcagagtt attgcatggc gatgtaaata taattaattg gtgtgaaaag tttgagccta
    17161 cagatgaaga caatgagcgt gctgttaaag agttatttaa gtcatttaaa aatataataa
    17221 atgagttata gttttagttg gttttatatt ggttaaaagc aaaatgctaa aaatttaact
    17281 ttgccgtcat tcccgcgtag gcgggaatcc atagtggaat ttacagaacc cgatatttga
    17341 aaagcagttg ccgaaattca aaaaatggat tcccgcctgt gcgggaatga cggcaaggtg
    17401 gcagtaaacg ttttaaacag tattgattgt caatgaaact caaaaggccg tctgaaaccc
    17461 atttttcaga cgacctccat aaaagattat ttatcaaata cccgtaacta ggaacgaacc
    17521 atgttacaaa tcgaaatcga cggcaaacaa gtatctgtgg agcagggcgc gacggtgatt
    17581 gaagccgcgc acaagctcgg tacttatatt ccgcatttct gttaccacaa aaaactttcc
    17641 atcgccgcca actgccgtat gtgtctggtg aacgtagaaa aagccccaaa acccctgcct
    17701 gcctgtgcca cgccggttac agacggcatg attgtgcgta cgcattcggc aaaagcccga
    17761 gaggcgcagg aaggcgtgat ggagttcctg ctcatcaacc atccgcttga ttgtccgacc
    17821 tgcgaccaag gcggggaatg ccagttgcag gatttggcgg tgggctacgg aaaaaccacc
    17881 agccgctaca ccgaagaaaa acgttccgtc gtcggcaaag atatggggtc cttggtttcc
    17941 gccgaggaaa tgagccgctg tatccactgc acccgctgcg ttcgtttcac tgaagaaatc
    18001 gccggtttgc aggaaattgc gatggtgaat cgcggcgaac actccgaaat catgcccttt
    18061 atcggcaaaa cggtggaaac cgaattgtcg ggcaacgtca ttgatttgtg tcccgtcggc
    18121 gcgctgacca gcaaaccgtt ccgcttcaat gcgcgtactt gggaattgaa ccgccgcaaa
    18181 tccgtttccg cccacgacgc tttgggcagc aacctgattg tacaaaccaa agaccatacc
    18241 gtccgccgcg tgttgccgct ggaaaatgaa gcgattaacg aatgttggct gtctgaccgc
    18301 gaccgttttt cctacgaagg cctgtatcac gaaagccgtc tgaaaaaacc gaaaatcaaa
    18361 cagggcggcg agtggatgga cgtggattgg aaaaccgcgt tggaatatgt ccgcagcgcg
    18421 attgaatgta tcgccaaaga cggcaaccaa aaccaagtcg gcatttgggc gaacccgatg
    18481 aatacggttg aagagctgta tctggccaaa aaacttgccg acggcttggg tgttaaaaac
    18541 tttgccaccc gtttgcgcca acaagacaaa cgtctttcag acggccttaa aggtgcgcaa
    18601 tggttgggac aaagcattga atcgctggcg gacaacgatg ccgtattggt agtcggtgcg
    18661 aacctgcgca aagaacagcc gctcctgacc gcccgcctgc gccgcgccgc caaagaccgt
    18721 atggcgttga gtgtattggc cggcagtaaa gaagagttgt tgatgccgct tctctctcaa
    18781 gaagccgcac atcccgacga gtgggcaggc cgtctgaaaa acctgtctgc caatgcggaa
    18841 cacgccgtta ccgccagcct gaaaaacgct gaaaaagcag cggtgatttt gggcgcggaa
    18901 gtgcaaaacc atcctgatta cgccgccatt tatgccgccg cgcaagagct ggcggatgca
    18961 accggcgcgg tactgggtat tttgccgcaa gccgccaaca gcgtcggtgc ggacgtattg
    19021 ggcgtgaact caggtgagag cgttaccgaa atggcaaacg cgccgaaaca ggcagtcttg
    19081 ctgctcaacg tcgagcctga aattgatacg gttgacggtg caaaagccgt agccgcgttg
    19141 aaacaggcaa aaagcgtgat ggcgtttaca ccgtttgtca gcgaaacgct gctggacgtg
    19201 tgcgacgtgt tgttgccgat tgcgccgttt accgaaacct caggcagctt catcaatatg
    19261 gaaggccgtc tgcaatcttt ccacggcgtg gtacaaggct tcggcgattc gcgtccgatg
    19321 tggaaagtgt tgcgcgtatt gggcaatctg tttgacctga aaggttttga ataccacgat
    19381 accgctgcga ttttgaaaga cgcgctggat gcagaaagcc tgtcgtccaa actggacaac
    19441 cgcagtacat ggacagggga gggcgttcag acggcatcag accgcctcgt ccgtgtcggc
    19501 ggcgtcggta tttatcacac cgatgccatc gtacgccgtt ccgcaccgtt gcaagaaacc
    19561 agccatgccg ccgtgcctgc cgcgcgtgta aatccgaaca cattggcacg cttgggcctg
    19621 caagacggac aaaccgctgt cgccaaacaa aacggcgcaa gcgtatcggt tgctgtcaaa
    19681 gccgatgccg gtttgcctga aaacgtggtg catctgccgc tgcataccga aaatgccgcg
    19741 ctgggtgcgt tgatggacac tattgaactg gcgggagctt gatcatgcaa gaatggttcc
    19801 aaaacctctt tgccgcaacg ctcggtctgg gcgatttggg catcaccgta ggcttggtgg
    19861 tatccgtcat cgtcaaaatc gtgattatcc tgattccgct gattctgacc gttgcctacc
    19921 tgacgtattt cgaacgtaaa gtcatcggct tcatgcagct tcgcgtcggt ccgaacgtaa
    19981 ccggcccgtg gggtctgatt cagccgtttg ccgacgtgtt caaactcttg tttaaagagg
    20041 taacccgtcc gaagctgtca aacaaagccc tgttctacat cggcccgatt atgtcgcttg
    20101 ctccgtcttt cgcagcatgg gcagtgattc cgttcaacga agaatgggtg ctgaccaaca
    20161 tcaatatcgg ccttttgtac atcctgatga ttacctcgct gtcggtttac ggcgtgatca
    20221 tcgcgggctg ggcttccaac tccaaatatt cgttcttggg tgcaatgcgt gcttccgcac
    20281 aaagcatttc ctacgagatt gccatgagtg ccgcgctggt gtgcgttgtg atggtatcgg
    20341 gcagcatgaa cttctccgac atcgttgccg cacaagccaa aggcatcgcg ggcggttccg
    20401 tgttctcgtg gaactggctg ccgctcttcc ccatcttcat cgtttatctg atttccgccg
    20461 ttgccgaaac caaccgtgcg ccgtttgacg tggcagaggg tgagtctgaa atcgttgccg
    20521 gtcaccacgt cgaatattcc ggcttcgcgt tcgcgctgtt cttccttgcc gaatacattt
    20581 tcatgattct gattgccgcg ctgacatcgc tgatgttcct cggcggctgg ttgtccccgt
    20641 tcccgcaaag ttggggcttt atcggcacac cttccgcatt ctggatgttc gtgaaaatgg
    20701 cggcggtgtt gtactggtac ctgtggatcc gtgcaacctt cccgcgctac cgttacgacc
    20761 aaatcatgcg tttgggctgg aaagtgctga ttccgatcgg cttcgcctac atcgtggttt
    20821 tgggcgtgtg gatgatttca ccgctgaatt tgtggaaata agtttcagac ggcatcttga
    20881 ggccgtctga acaaagcgat tttgaatacc taacgaaatc cctgttttga gggaacataa
    20941 tatggctaac ttagtaaaaa cctttctgct tggcgaattg gtaaaaggta tgggcgtaac
    21001 gctcaaaaac tttttcgccc gcaaagacac gatttatttt cccgaagaga aaacgccgca
    21061 atccgtgcgt ttccgcggcc tgcacgcgca gcggcggtat ccgaacggtg aggaacgctg
    21121 cattgcgtgt aagttgtgcg aggcggtgtg tccggcaatg gcgatcaata tcgaatcgga
    21181 agaacgcgaa gacggcaccc gccgcaccaa gcgttacgac atcgacctga ccaagtgcat
    21241 cttctgcggc ttctgcgaag aggcctgccc aaccgatgcg attgtggaaa cgcatatttt
    21301 tgaataccac ggcgagaaaa aaggcgactt gcacatgacc aagccgatcc tcttggcaat
    21361 cggcgacaaa tacgaagccg aaatcgccaa acgcaaagcc gctgacgcgc cgtaccgtta
    21421 aggagcagac gaatgacttt ttccgcgatt ctgttctata tccttgccac catcgtctta
    21481 tacggcgcgc tgcgtaccgt taccgctaaa aaccctgtcc acgccgcttt gcatctggtg
    21541 ctgaccttct gcgtgagcgc gatgctttgg atgctgatgc aggctgagtt tttgggcgtg
    21601 acgctggtgg tggtttacgt cggcgccgtg atggtgttgt tcctgttcgt cgtgatgatg
    21661 ttgaacatcg acattgaaga aatgcgtgcc ggtttctggc ggcacgcgcc tgttgccggt
    21721 gtggtcggca cattgttggc ggttgcgctg atcctgattc tggtcaaccc gaaaaccgac
    21781 cttgccgcat ttggtctgat gaaagacatt cctgccgatt acaacaatat ccgcgatttg
    21841 ggcagccgta tttataccga ctatctgttg ccgtttgaat tggcggcggt attgctgttg
    21901 ttgggtatgg tggcggcgat tgcgctggtt caccgtaaaa ccatcaatcc gaaacgtatg
    21961 gatcctgccg accaggttaa agtacgcgcc gaccaaggcc gtatgcgtct ggtgaaaatg
    22021 gaagcggtca aaccgcaagt cgaatctgcc gaagaaagcg aagtttcaga cggtctcaag
    22081 ccggaagggg agggcaaagc atgattacct tgacgcatta tctggtattg ggcgcgctcc
    22141 tgttcggtat cagcgcgatg ggcatcttta tgaaccgcaa aaacgtgctg gtcttgctga
    22201 tgtccatcga gctgatgctt ttggcggtga acttcaactt tatcgccttc tcgcaacatt
    22261 tgggcgatac cgccggacag attttcgtat tcttcgtact gaccgttgcc gctgccgaat
    22321 ctgccatcgg tttggcgatt atggtattgg tgtaccgcaa ccgacaaaca atcaacgttg
    22381 ccgatttgaa caaacttaaa ggttgatttg gaatttttaa agaaaaaagg agatagataa
    22441 tgtttgatat tgctgctgtt ggattaggtg cagcaggagt tagcttaatc aaacagttac
    22501 aggtaaaaac tcttgtttgc catatcaagg cactttggtc tccacacgcc catatatgca
    22561 taaattgtgg attagtatga gtgaaagaga atgtttacaa tttcaagggc tcattggcag
    22621 ccattgggct gtatggcggg taattgggta aaacaatggg tttttgaaaa ttttagaatt
    22681 tcagaaagtg gagagtgatt atgtcatttt caattgctct tccattggta tatctgttta
    22741 ttggcatttt gattgggaaa acccctttta gaaaaattaa aggcactttt tctgtaatat
    22801 tgagcagatg gattatccct tttgttattg tctataacat aacgacatat cgtgatggta
    22861 tgcagtatgt tatgttatgg atgattattt cgatggggtt gattatccta atcgggaaaa
    22921 gagttgataa agatcctgtt tggaatttgt gtttgtgcta tttgaacatc ggatggttgg
    22981 ggcttcccat agttgcaacc ttgtttggag atgaggcggc gcaagtgctt atttcggcat
    23041 acgtgggaag ttctttattc ggaaattcgg tcggggtcgg gttgttattg aataatagta
    23101 atttcatatc cggattgaaa aagactttaa aatcccctcc cgttgttgca ttggtaatag
    23161 gaatgttcgg tattccgcta gggcattttg tagcagatta ttttggggaa gtttattttt
    23221 tctcgaaaat agcaatggga atattgggga tggtgatact cggtatttgg ctggctgaga
    23281 taaaactaaa tttgaatgat ttgaagaaaa gtattaaacc gtttctaata aaaaacattt
    23341 tatttatagg gattgttttt ctttttatcc aaatagctgc atattttgac ttgaagttga
    23401 ttcttgaaaa ccaaaaaaca ttgtatttaa taccgttgct gccgcctgct gccaatatta
    23461 ttgtgttgga aacggcatat ttgaaaacag ggcgttcggc ttctatgatt gcgtatggta
    23521 cgatttttag tttaatcgca atcagcattt atattttact ggtgcaaatt taaatgaacg
    23581 acatgacttt atatctcatc atcgccctcg tgcctttggc aggctcgctg attgcgggtt
    23641 tgttcggcaa caaaatcgga cgtgccggcg cgcatacggt tacgatactc ggcgtggctg
    23701 tgtccgccgt gctgtcggct tatgtgctgt ggggattcct caatggcagc cgtgccaagt
    23761 ttgacgaaaa cgtctatacc tggctgacaa tgggcggctt ggatttttcc gtcggcttct
    23821 tggttgatac gatgacggcg atgatgatgg tcgtggtaac gggcgtgtcg ttgatggtgc
    23881 atatctacac catcggctat atgcacgacg aaaaagtcgg ctaccaacgc ttcttcagct
    23941 acatttcttt gtttacattc agcatgttga tgctgattat gagcaacaac ttcatccagc
    24001 tcttcttcgg ttgggaagcg gtgggtttgg tgtcgtatct cttgatcggt ttctatttca
    24061 aacgcccgag cgcgacattt gccaacctga aagccttttt gatcaaccgt gtcggcgact
    24121 tcggcttttt gctcggtatc ggcttggtgc ttgcctattt cggcggcagc ctgcgctatc
    24181 aggacgtatt cgcctatctg cccaacgtcc aaaatgccac catccagctt ttccccggcg
    24241 tggaatggtc tttgattacc gtaacttgtt tacttctgtt tgtcggtgcg atgggtaaat
    24301 cggcacaatt cccgctgcat gtctggctgc ccgattcgat ggaaggcccg accccgattt
    24361 ccgcattgat tcacgccgca accatggtta ccgccggtct gttcatggta tcgcgtatgt
    24421 cgccgattta tgagatgagc agcactgcgc tgtctgtcat catggtaatc ggtgcgatta
    24481 ctgctctgtt tatgggtttc ttgggcgtga ttcaaaacga catcaaacgt gtggttgcgt
    24541 attccaccct gtcgcaactg ggctacatga ccgtggctct gggcgcgtct gcctattccg
    24601 tggcgatgtt ccatgtgatg acccacgcct tctttaaagc cttgctgttc ttggcggcgg
    24661 gcagcgcgat tatcggtatg caccatgacc aagatatgcg ccacatgggc aatctgaaaa
    24721 aatacatgcc gattacttgg ctgaccatgc tgatcggtaa cttgtcgctg atcggtacgc
    24781 cgttcttctc cggcttctat tccaaagatt cgattatcga agcggcgaaa tacagcaccc
    24841 tgccgggcag cggctttgcc tattttgccg tcctcgccag cgtttttgtt actgcgttct
    24901 acgcattccg ccaatacttt atggtgttcc acggcgaaga gaaatggcgc agcttgcctg
    24961 aacatcatga cgatcatcat ggcgaagaac atcacagctt gggcaaaaac gacaatccgc
    25021 acgaaagccc gttggttgtt accctgcctt tgattttgct tgccattccg tccgtcatca
    25081 tcggctacct tgccatcgag cctatgcttt acggcgattt cttcaaagat gtgattttcg
    25141 tcaataccga cgcgcatccg accatgcaca tcatgaagga agagttccac ggcgcattgg
    25201 caatggtgtc gcacagcctg cattcgcccg tactctacct tgctatcgca ggcgtgttga
    25261 gcgcatggct tttgtacgtc aaactgccgc acctgccagc gaaaattgca cagacgttcc
    25321 gtccgattta cgttttgttt gaaaacaaat actacctcga cgccctgtat ttcaacgttt
    25381 tcgccaaagg cacacgcgca ttgggtacct tcttctggaa agtcggcgat accgccatta
    25441 tcgataacgg catcgtcaac ggctctgcca aactggtcgg cgcgattgcc gcgcaagtgc
    25501 gtaaagccca aaccggcttt atctacacct acgccgccgc tatggtattc ggcgtattgg
    25561 tactgctcgg catgaccttc tggggattgt tcagataaag gcggagtttc agacgacctg
    25621 ttcatagttt tgtagaggtc gtctgaaaag tttgagaagt gggcattaag tcaaagggtg
    25681 gaacaaaatg gtacccaaca ggcgtgaaat agccaacatg caaattatct gtttcctaaa
    25741 ttttttaggc aatctcaaca aatatagagg agtcaaaagg gatgacacag ttatttttac
    25801 attcagatac accaaatgag aaggaactga tttcaatata tgagaaagct gtttcagagg
    25861 cagtagagct gtatattttc tctgcgtatc tcactcattg ggatgtaaaa aataagctca
    25921 atagcaaatt gcaagagttt aaatttattt ttggacaaga tttcggattg tctaaaaaag
    25981 aggctattcg gaaagtgttg aaatggcttc cgtcccattt gaaattcaca ctgatggtag
    26041 ctcaaggcat tcagggtttt catcctaaag ctgtattttg gaagaatgat aagaatgaat
    26101 attatgcttt aatcggttct tcaaatttga ctcatgctgc gtttaatagc aattatgaag
    26161 caaatatttt gactaaaatt tccgagcagg attttataaa agtcaaatct tgggctgatg
    26221 aaattgcgat gaaatctatc ccagtatcgg aagattggct tgaggagtat caggaagctg
    26281 aaattaatta taagaaatca actgttagcc aatcattgat ggataagtta tttatggaaa
    26341 tgccaaatta taatcaagaa ctgattgcag cacggagaaa acagatgaga aaccatcaaa
    26401 ctgtgtgtaa tcaattaaaa agtcttatta agcaatgtgc tgcaggaaag atagacaatg
    26461 atgagtttta tgtggaattt aataaattat ggtcttggaa aagcgaaaat aaaggagaag
    26521 gggtaggtaa tcgatttcaa gatcaaacgt ggaaaaggac cggaaagtct tcagatttta
    26581 gaaaattatg cattgctatc caatcagttt ttgatgcacc acttgctgag agagataatg
    26641 ttgttgccaa gcaaattgat tggctaaaag aatgtggagt ttccactcgt ggttcagttt
    26701 tttctgaaat gctttgtcaa gaatatccag atagatatcc agtgttaaac gctcctatta
    26761 aaaaattttt agaaaaaaat aaatttaagt ctgcaaaagg agcaagtgaa ggctctagat
    26821 atattgattt aagtatgaag ttaagagctt tactagcaat gcagtcagaa ataaaaaatt
    26881 tggctgaatt agatgtttta gtgcaggcag aatatcgcga tagtacagat attgattggg
    26941 agtgagtaaa gaggggagag agtattcatc cctttttcag acgacctcct gaaacgtcgt
    27001 ctgacaaact aacccgcact acgatatttt tattttaacc acaggttaac cactatgttt
    27061 tccaactacc tactcagctt ggcaatatgg atacccatcg ccgcaggtgt gctggttttg
    27121 tcgaccggta aggacagccg tgcgccgctg gcgcgtgtgc ttgcctttat gggtgcgctt
    27181 gccggtttct tggtaacgct gcccctgttt accggtttcg accgtttgag cggcggctat
    27241 caatttaccg agttccacga gtggattccg ctgctgaaaa tcaactatgc attgggcgtg
    27301 gacggtattt cagtgctctt tatcatcttg aatgcgttta ttacgctgct ggtggtattg
    27361 gccggttggg aagtcattca gaaacgtccg gcgcagtata tggccgcttt cctgatgatg
    27421 tcgggtttga ttaacggcgc gtttgccgct caggatgcga ttctgtttta tgtgttcttt
    27481 gagggtatgc tgattccgct gtacctgatt atcggtgtat ggggcggccc gcgccgcgtc
    27541 tatgcgtcgg tcaagctgtt cctctatacg ctgatgggtt cactcttgat gctggttgcc
    27601 atggtttacc tgtactatca aacaggcagc ttctctattg tcgatttcca aaacatcaaa
    27661 cagattccgt tgggcgtaca gcagcttttg tttgtggcat tcttcctgtc atttgccgta
    27721 aaagtgccga tgttccccgt acacacttgg ctgccggatg cccacgttga agcaccgacc
    27781 ggcggttcga tggtgttggc tgcgattacg ctgaaactgg gtgcgtatgg tttcttgcgc
    27841 tttatcctgc cgattatgcc ggatgcggca cgctattttg cccccgtgat catcgtattg
    27901 agtctgattg ccgtgattta tatcggtatg gtggctttgg tgcaaaccga tatgaaaaaa
    27961 ctggtggcgt attcgtccat cagccatatg ggttttgtaa cgcttgggat gtttttgttt
    28021 gttgacgggc agttggacga ctgggcattg aaaggcgcgg tcattcagat gatttcgcac
    28081 ggtttcgtgt cggcggcgat gtttatgtgc atcggtgtga tgtacgaccg cctgcacacg
    28141 cgcaatatcg ccgattacgg cggcgtggtc aacgtgatgc ccaagtttgc ggcgtttatg
    28201 atgttgttcg gtatggcgaa cgccggtttg ccggcgactt ccggcttcgt gggcgagttt
    28261 atggtgatta tgggcgcggt taaagtgaat ttctgggtgg gcgcgttggc ggctatgacg
    28321 ctgatttacg gcgcgtctta taccctgtgg atgtacaaac gcgtgatttt cggcgagatt
    28381 cgcaatccgc acgttgccga tatgaaagac atcaattgcc gcgaatttgc ggttttggcg
    28441 gttttggcgg cggctgtttt gggtatgggc ctgtatccga acgcgtttat cgaagtggta
    28501 catcaggcgg caaacgattt gattgcccat gtggcacaaa gcaagatttg aggtgtgtaa
    28561 atgaactggt ctgatttgaa tttaatgccc gccatgcccg aaatcgtgct gctgtcgctg
    28621 ctggtgttat tgttgctggc agacttgtgg gtcagtgatg acaaacgccg ttggacgcat
    28681 tacggcgcgt tggcaacggt ggcggttacg gctgtggtgc agcttgccgt ttgggaacag
    28741 ggcagcacgt cttcgttcaa cggcatgtat attgcagacg gtatgtcgcg tttggcaaaa
    28801 atggttttat atgccttgac ctttgccctg tttgtctatg ccaggcccta caaccaagtg
    28861 cgcggtattt tcaaaggcga gttttacacc ctgtcgctgt ttgccctctt gggtatgggc
    28921 gtgatggtga gcgcggggca ttttttaact gcctatatcg gtttggaact cttgtcgctt
    28981 gccctttacg ccctgattgc cctgcgccgc gattccggct ttgccgccga agccgccttg
    29041 aaatattttg ttctaggcgc gctggcatcc ggcctgctgc tctacggtat ttctatggtt
    29101 tacggcgcaa ccggttcgct ggaatttgcc ggcgtgcttg cctcttcctt caatgaagaa
    29161 gccaacgaat ggctgttgaa actgggtctg gtgtttatcg tcgtcgccgt tgggttcaaa
    29221 ctcggcgcgg taccgttcca tatgtgggtg cccgacgtgt atcacggcgc gcccacttct
    29281 gttaccgccc tagtcggcac tgccccgaaa gtcgccgccg tcgttttcgc tttccgcatc
    29341 ctcgttaccg ggctgggaac cgtgcatcat gactggtctc tgatgtttgc cctgcttgcc
    29401 gccgcctcgc tgctggtcgg caaccttgcc gccatcatgc agaccaatat caaacgtatg
    29461 ctcgcctatt ccaccgtatc gcatatgggt ttcatcctgt tggcgtttat ggcgggcgcg
    29521 gtcggctttg cggcgggtct ctattacgcc attacctacg cgctgatggc ggcggcaggg
    29581 ttcggcgtgt tgatggtgtt gtcggatggg gacaacgagt gcgaaaacat cagcgatttg
    29641 gcagggctga accaacaccg cgtatggctt gcctttttga tgttactggt tatgttctct
    29701 atggcgggca ttccgccgct gatgggtttt tacgccaaat ttggcgtgat tatggcactc
    29761 ttaaaacaag gctatgtttg gttgtcggtg tttgccgtcg tgatgtcgct gattggcgcg
    29821 ttctactacc tgcgcgtggt caaagtcatg tattttgacg aatccggccg cgcccgtctc
    29881 gccgccggcg gcaacaatgc cgcaaaattc ctcttgagcg tcaacgcgct gctgttggtg
    29941 ctgtggggca ttatgccgca aaccgttatc gactggtgcg ccaaggcgtt ggaaaacacg
    30001 ctgtaagccg ccgcaacggc agccgtgtca gaggctgccg tttttgttaa gatatgccgt
    30061 tccgcaatgc agttcagacg gcatcgccgc cgacaacgcc caaacagaaa gcccaccatg
    30121 accgcatcca tgtacatcct tttggtcttg gcactcatct ttgccaacgc ccccttcctc
    30181 acgaccagac tgttcggcgt ggccgcgctc aagcgcaaac atttcggaca ccacttgatc
    30241 gagctggcgg caggtttcgc gctgaccgcc gttcttgcct acatcctcga atcccgtgcg
    30301 ggagcggtac acaatcaggg ttgggagttc tacgccaccg tcgtctgcct gtacctgatt
    30361 tttgcgtttc cctgtttcgt gtggcggtat ttttggcaca cgcgcaacag ggaataggca
    30421 ggcataggaa tgccgtctga aaccctttca gacggcattt gtttcattca agtgcaggcc
    30481 ggcatcgctg tgccggcacg tttcagccgg cgatatacgc cggttttaat atttgcgggc
    30541 gactgcaaat tctgccaact gccgcaggcg cagggctttg tcgccgaagg gttcgagcag
    30601 cgcgaccgct tcggcaacca gtttgtgtgc gtatgagcgc gccgcttcca agcccatcag
    30661 tttcacataa gtcggcttgt cgttgtctgc gtctttgccc gccgttttgc ccaaagtcgc
    30721 cgtgtccgct tcacaatcca acacatcgtc aatgacttgg aacgccagcc ccagttttgc
    30781 cgcataatcg tccaataccg ccagactgtc ttcagacaga ttcgggcagg acatcgcccc
    30841 caataaaacc gccgcacgga ttagcgcacc cgttttcagg ctgtgcatct gttccaaatc
    30901 ggcttgaacc atttgtttgc cgacattcgc caaatcgatt gcctgaccgc ccgccatgcc
    30961 cgcgctgccg cccgctttcg ccaacaccga caacattgcc aactggcgtg ctgcgggcag
    31021 ttctgtcgga cggcttaaca cgtcgaaggc ttgggtttgc aaagcgtcgc cgaccagtag
    31081 cgcggtggct tcgtcgtatt gtatatggca agtcggtttg ccgcgccgca ggctgtcgtt
    31141 gtccatcgcc ggcatatcgt cgtgaaccaa agaatagacg tggatcattt cgattgccgc
    31201 cattgcctgt ccgaccgcat ccgccacggc tccgcccaat tccgaagccg ccagcaccag
    31261 catcggacgc agacgtttgc ccccgtccaa agccgcataa cgcatggctt cgtgcagtgt
    31321 gtgcggtatt tccccctcag acggtaaaaa ccgttcaagc agcagctctg tttgcgcctg
    31381 cgccctaagc tgccatgctt tcaaatcatt cgtcggattc aaggttcaac tccttcagcc
    31441 cgtccgcatc caaaacctgt aatttctgtt cgacttgtgc cagtttggtt tggcagtacc
    31501 tgaccagctc gttgccttcc tgataggcgg caagcgcgtc ttccaagggc atttcgccct
    31561 gcatagactg cgtcagcgat tcgaggcgcg acaaggcttc ttcaaacgat ttcggggcgt
    31621 ttttcttcat cgtatttcct tttcggttga aaccccgccc tttagggcgg caggatcaga
    31681 ctttatttgg gaggggtgta accctttcca aatcagggca atacataggg cggtgcttta
    31741 tgtgccgtcc tgtgtgttgg aacatagttt cggatgttcc ggtaaaaagc ggattgtagc
    31801 atttttgaaa aacggatgcc gtctgaaacc cgaatccggc ttcagacggc attttttccg
    31861 cccaggcggc aaggcgttac ccgggcagtt cgtcggtgat gccctgcaaa aaggcgaggc
    31921 gttcggggct tgccgccccg gtttgcgcgg cggctttgaa ggcgcagccg ggttcggcgc
    31981 ggtgggtgca gttgtggaag cggcattgcc cgacaaggtg gcggaaatcg gggaaatagc
    32041 gcggcaaatc ggcggcttgg aggtggtgta aaccaaattc ttgcaaaccc ggggagtcga
    32101 tgagttgggt ttcgccgttc aaatcataaa gccgggcgtg ggtggtggtg tgttttcccg
    32161 agtcgagtgc ggcggaaatg tcgccggtgc gggcggtttg gctgcccaaa agggcgttgg
    32221 tcagggtgga tttgcccata ccgctctgcc cgagcaggat gttgctgtgc ccttgcaggg
    32281 cggggcgcag gctgccggcg ttttccagtg cgcgggtttc gatgacggga taacccagcg
    32341 tttcgtagaa tttgagtttt tcgcgccaaa gggcggtttc gggcaggtcg gctttgttca
    32401 ggacgatgac ggcttcaata ccggcggctt cggcggcaag cagggcgcgt tgcagcagcc
    32461 gcacgctcgg actcgggacg gcggcggtta cgatgaggag ttgggtaacg ttggcggcga
    32521 tgagtttggt tttccacgcg tcttggcggt agagcaggct ttggcgcggt aaaaaatctt
    32581 caatcacaac ttgttcggcg ttgacggggc tgatgcggac acggtcgccg caggcgaaat
    32641 cgacgcgttt tttgcgggtg ctggcttcgt aggttgtgcc gtcgggcgtg cgcacgatgt
    32701 agcggcggcc gtagctggcg gtaatttggg cggtgtcgtt catggtttct ttggggttgg
    32761 gtgtggaaat gccgtctgaa aacgggtgtt cggacggcat cggttcagtc gtgctgccac
    32821 tcgacgtgtt cgttgaggaa gccgccgctc tggtgcgccc agagtttggc gtaaagcccg
    32881 cgtttttcga ggagttcggc gtgtgtgcct tcttcgatga tgcggccttt gtcgaggacg
    32941 acgagcctgt ccattgcggc gatggtggag aggcggtggg cgatggcgat gacggttttg
    33001 ccgtccatca ttttgtcgag gctttcctgg atggcggctt cgacttcgga atcgagcgcg
    33061 ctggtggctt cgtcgagcag caggatgggc gcgtctttga gcatcacgcg ggcaatggcg
    33121 atgcgctggc gttgcccgcc ggagagtttc acgccgcgtt cgccgacgtg cgcgtcatag
    33181 ccgcgccgcc ctttggcatc ggaaagttcg gggatgaagc cggcggcttc ggcgcgttcg
    33241 gcggcggaaa ccatttcggc atcggtcgcg tcggggcggc cgtaaataat gttgtcgcgc
    33301 acggaacggt gcagcagcga ggtatcttgc gtgaccaaac cgatttgggc gcgtaaagat
    33361 tcttgggtaa cgccgcttat gtcctgcccg tcgatcgaaa ccgtgccgct ttgcggttcg
    33421 tagaagcgca aaagcaggtt gacgatggtg gatttgcccg cgccgctgcg tccgatcaag
    33481 ccgacttttt cgcccgggcg gatggtgagg ttgaagccgt tgagcagcgg tttgccggct
    33541 tcgtagcaga aatcgatgtg ttcaaatttg atttcgcctt gcgacacttt cagcggcagg
    33601 gcgttcggct tgtcgaggat ggtgtgcggt ttggacaggg ttgccatgcc gtcgccgacg
    33661 gtgccgatgt tttcaaacag ccgcgcggat tcccacataa tgtattgcga cagcccgttg
    33721 acgcgcaacg ccatcgcggt ggcggtggcg accgcgccca cgccgacctg cccgttgtgc
    33781 cagagccaga tgcccagtgc ggcggtggag agggtcaggg aggtgttgac gatgaagctg
    33841 cacgaatgca gcagcgtcgc cagccgcatt tgggcgcgca ccgtaaccat aaattcttcc
    33901 atcgactgct tggcataggc ggcttcgcgc gcgccgtggg agaagagttt gacggtggcg
    33961 atattggaat aggcatcggt aatgcggccg gtcatcagcg agcgggcatc cgcctgccat
    34021 gcggcggttt gccccaattt gggaatcagc aggcgcatca ccgaagcgaa accgacaatc
    34081 cagccgataa agggcagcag cagccatgag tcgagcgagg cgagaatcac gccggaggta
    34141 atgaaataca ccgacacata aacgaccata tcggcaaccg tcatcaccgc gtcgcgcaac
    34201 gccagcgcgg tctgcatgac tttggcggac acgcgtccgg caaattcgtc ctgataaaaa
    34261 ccgaggcttt ggttcagcat caggcggtgg aagttccagc gcaggcgcat agggaacacg
    34321 ccctgaaggg tttgcaggcg cacgttggac gcggcatacg cccacacgac cgaaaacacc
    34381 atcatcgccg ccattgccgc cagttcccaa cctttttcgg caaacagttc ggcgggcgcg
    34441 tatttgccga gccactccac gattttgccc ataaattgaa aaaccagggc ttccataatg
    34501 ccgatgccgg cggtcagcgc agccagggcg gctatccatt tccgcacgcc ggccatgctg
    34561 ctccagacaa accgccacaa gcctttttct ggcgttttcg gggcggcttc gggataaggg
    34621 tcgattcggg actcgaacca ggaaaatatt ttgttcaaca ttgttttcga tttcggtaaa
    34681 acagtttcag acggcatcaa acacaatgcc gtctgaaagg aaggacaata acgccatttt
    34741 acgggaaaag ccgtcgggaa gacagcgcga ggcggaaacg cagggtttcg tcagggcaaa
    34801 cgccgcgccg ccttcagacg gcattatttc agcaggtttt tcaaagcaag gcgcacgcct
    34861 tcgcccacgt ccgtcccttt cggaacgcct ttgaccgccg cttttgcttc gcgttcgctg
    34921 taacccagtg caagcagtgt gctgacgatg tcttccgttt cgtcggcggc gggtgcggcg
    34981 gcaaacagcc cgtccgttac cgtatgcgcg accagcttgc cgcgcagttc caagaccata
    35041 cgttcggcgg tttttttgcc gattcccggc gcggaagaca ggcgtttgac atcttcttct
    35101 gcaaccgccc gcgccagctc gtctgccgtc atcgctgaca aaatgcccaa agccgttttc
    35161 gcgccgatgc cgccgacctt aatcagttgg cggaaggtct tgcgttcttc cgcagtggca
    35221 aaaccaaata aaagatgtgc gtcttcccga atgataagct gggtaaacag ttgtacgctt
    35281 tcacccacgg gcggcaggtt gtagaaggtc tgcatcgata cgtcggcctc atagccgaca
    35341 ccgttgacat cgatgacgat ttgcggaggg tttttttcaa ccagtttgcc ggtcagtctg
    35401 ctgatcatgt atgaaggatc ctgaagtgtc gggtgcaaaa tgccgtctga aaccggttcg
    35461 ggcttcagac ggcacggatt gtatcaaatt cagtcgtcgc ggcgggagga aatcacgcgg
    35521 ccggtacggg catcgacaac gactttgtat tcctgtccgt ttttgacgat ttcgacatca
    35581 tagtgcggac ggccgttgtc gtgttcgaga tcgatgtcgg tgattttgcc gccgacacgc
    35641 gccaacgctg ctttttcggc ttgggcgcgg ctgatgattt tgtcttgttt gctgtgttgg
    35701 tgtgcggcgt gtccgtggtc gtcatcgccg tgtccgtcgt ggtgggcgag tgcgggggcg
    35761 gaaatgccca tcagtgcggt tgcggcggcg gtcaagagaa ggtgtttgat gttcatattt
    35821 tgcctttgta aatcgtgggt tggaaaatgt ggatattaat aaggtatcaa ataaccgtca
    35881 gccggcagtc aataccgccc gaaccatacc gcgcgcctga gcttcggctt cggcggcgcg
    35941 ttcctgcgag gaaaacggcc ccattttgac gacatattcg taatggcgtt tttcaaccga
    36001 taggttcggg tttgatgccg atgaagccag gttttgggcg gcttggttca gataggcttg
    36061 tgcttcgtgt tccgtaccga aagatttcaa gtcgatgaag atgtctttgt tttcggcaac
    36121 cagtgcggat tggcccggga cgatttgttc gattttgacg tgcgccgtcc cttggctgac
    36181 aaagcccaat ttttgcgcgg cggctttgga tacgtcgatg atgcggttgc cgtggaaggg
    36241 accgcggtcg ttgacgcgga cgatgacgct tttgccgttt ttggtattgg ttacgcgcac
    36301 atagctgggg atgggcaggg ttttgtgggc ggcggtaaag gcgttcatat cgtatcgttc
    36361 tccgccggaa gttttgcgcc cgtgaaacct gccgccgtac cacgaggcgt tgccggtttg
    36421 cgtgaattcg gcgacttggt ttttcggcgt gtagcgtttt ccggcgactt tgtagctgcg
    36481 gttggcggag gcgtgcagtt tttctgcctt gaccactgcg tcggcggatg ccgtctgaag
    36541 ggagtgtgtg ccgaatgcgg cggtgagaag gaaaagggtt tttcgggtta aagtcaaaac
    36601 gtgttccgtt cttgagttga agacgaatgg gcatcatgcc cgccggatac gttccgaacc
    36661 gccgtacagt gcggacggcg gttcggaatg tgtccggata ggttttcaga cggcatgaac
    36721 ttgcgttcaa acgccgcctg cgtaaccgtg ttgccgccac gcttcaaaga gaatcacggc
    36781 aacggtattg gaaaggttca tactccggct gccgggctgc atcggcaggc ggattttttg
    36841 cgcggcgggc aggctgtcga ggatgtcggc aggcagtccg cgcgtttccg gcccgaacag
    36901 taaaacatcg cctttctgaa acgcggtttc atcggggcgc gccgtgcctt tggtggtcag
    36961 ggcgaaaatg cgcctgcctg cgagtgcctt gaggcagtcg tcgaagtttt cgtgcaccgt
    37021 caggctggcg aattcgtggt agtcgagccc ggcgcgtttc attttggcgg aatccaatgg
    37081 gaagccgagc ggtttgacaa ggtgcaaatc cgcgccggta ttggcgcaca ggcggatgat
    37141 attgcccgtg ttcggcggga tttccggttg gtataaaacg atggtaaaca taaatatcaa
    37201 tcacttatag gcgcgtaacc ttgccacaag gcggatgggg tgtcaaaaaa tttagttatt
    37261 ttttcattgg cgtgcgtgcc agcgtccagc agcagattcg gtttgcgccc gattttttca
    37321 gcgtctttgc cagttcgtcc agcgtcgcgc cggtggtaaa gacatcgtcg attaacaaaa
    37381 tattacagtt ttccggtatc ggtgtgcgga tttcaaaggc gtttttgatg tttcgccgcc
    37441 gttcgccgcc tttgagcgtg ctttgcggcg ggcggtggtg tcggaaaacg gtgtgtcggg
    37501 gcagtatctg ccagccgtag cgttgtgcca gcagcccgac gatgctttca ctttggttga
    37561 acccgcgttg cagcagccgc tccctgctta gcggtacggg caggacgaaa tcgaaacatt
    37621 cgtctgcaag ccggtcgggc ggattctgca tcatcaggtc tgccagcggc tgcaccatgc
    37681 tcaaatcagc caagtgcttc agcgcgtgta tcatattgct gacgggcggt tcgtaatgca
    37741 gcgaagccca catccggtcg aatgcgggcg gttttttctg acagccgccg cacaccgcgc
    37801 cgccttgaac gtgcatgaaa cacagggggc agctgtttgc cgcgtcggtg cggtatgccg
    37861 ccaaatcgtc gcggcagccg gcgcagatgc cgtctgaaac gccggacgaa ccgtggcata
    37921 atacgcaacg cctgatagtg ggcgcgtctg cgatgcgccg ccaacaagag agaaaatcca
    37981 tgcctgatgc cgtcaaaaaa gtttacctga tacacggttg gggggcgaac cgccacgtgt
    38041 tcgacgattt gatgccgcgc ctgcctgcaa cgtggccggt gtccgccgtc gatttgcccg
    38101 gacacggcga cgcgcccttc gcgcaaccct tcgatattga ggcagccgcc gacgctgttg
    38161 ccgcacaaat cgatacgccc gccgacattc tcggctggtc gctcggcgga ttggtcgcgc
    38221 tgtatctggc ggcgcgccat cccgacaaag tccgttcgct ctgcctgacg gcgagtttcg
    38281 cacggctgac ggctgacgaa gactatcccg aagggcttgc cgcgcctgca ttgggcaaaa
    38341 tggtcggcgc gttccgaacg gattatgcca aacatatcaa acagtttcta caactccagc
    38401 ttttgcacac gcctgatgcg gacggaatca taggcagaat cctgcccgat ttggcgcgct
    38461 gcggcacgcc ctccgcctta caggaagctt tggacgcggc ggaaagggcg gatgcgcggc
    38521 atttgttgga caagatagat gttccggtac tgctggtgtt cggtagcaaa gacgcgatta
    38581 cgccgccgcg catgggcgag tatctgcacc gccgtttgaa aggcagcagg ctggttgtga
    38641 tggaaaaggc ggcgcatgcg ccgtttttga gccatgcgga agcgtttgcc gcgctgtacc
    38701 gcgactttgt cgaaggggtt tgacatgaat ccgcaggaca aatgctggca ggttcaccgc
    38761 catctcgccg aatatgccga ccaacgcctg acactcgtcc gcaacgcgcc caagtatatc
    38821 ctgcttgcag gtgcggatgc ggacatcagc cgcagcctgc tggcgaaacg ctatccgcag
    38881 gcggtatttg aagaatatga ttcccgtgcg gattttttgg cggctgccgc tgccgcccgc
    38941 aaaggcggtt tttggcaaag gtttacgggt aagggcgtgg tgcaacactg ccaatccccg
    39001 atcgcgccgc tgcccgaagc gtgtgccgat atgttgtggt cgaatctcgg actgttggcg
    39061 gcggaacaaa tccttcctgt gctgcacaac tgggcgcgcg ccttgaagac ggatgggctg
    39121 ctgtttttta cctgcttcgg gcgcgacact tttaccgaac tgaaatgccg tctgaaagaa
    39181 aacggcattg aaagccgcag cgtgatgttt cccgatatgc acgacttggg cgatatgctt
    39241 gctgaaaacg gcttttacga ccccgttacc gatacggcga agctggtgtt ggattacaaa
    39301 aaggcggaaa cgttttgggc ggatatggac acgctgggcg tttggcgggc gatggcgtgg
    39361 aacgatgaaa acgccgcgcg ttcgtgtgtc gggacaatat ttgaacggga aggcggtttg
    39421 agtattacgc tggaaacagt gtacggacac gccgttaaaa aactgatgct gccgcaaggg
    39481 gagaacgtgg tgcagttttt tccgaagaga tgatgtgcag atgccgtctg aagccgtttc
    39541 caagtttcag acggcatttg tttgtgaaaa ccgacagaac taaaggaaat gccgatgtat
    39601 agtgaattaa atttaaacca gtacagcgtt gcctcgcctt gccgtactat ttgtactgtc
    39661 tgcggcttcg ccgccttgtc ctgatttttg ttaatccact atatgctgat gccggagacg
    39721 tatattgcgt ctataacatc agactgaagc agtacactgc ctgccaggtt acccgagttg
    39781 aagaacacgg tggcaaaaaa acacatgcga ccctgctggc tttggactgg cagggcaaca
    39841 aaccgcttgg ggcggaggag ctggcggatt tgaaaccact ttacaaagac ttaaagaata
    39901 atattggaaa tattgtatga acaaaaaatt aaactatatt tttatgttgg actgtttagg
    39961 gttggtgata ttgtttactt gtataatagc tacttttgaa agagattatg gatttaaaat
    40021 ttttactaat tctaagaggc ctgaatttta ttattggatt ggaatgtttt attatggaat
    40081 tatttcttgc tggtttgatt atcaattaat ttcaacaaag gcgaattcgt ataaaagaaa
    40141 agttaaacaa tataaaattt tttcagtaat attttcagtt ttgatattta tttctactat
    40201 agtaaaactt taaattttgg agcaaaaatt tatgagcgat tcaattgaat atgtattggg
    40261 aacgcggtct gcacatgtat aaggcaagtg ctgtcgtgcc aacgggatat gtacgggttg
    40321 ggaataccgc gccgctggtc ggcgaagaca cgcaacggta tgcctctttt tggggcgacg
    40381 gctacgacgt gtaccgtcag ttgagatggc agcagatacc cgaaaaacag agaaaggcat
    40441 tcaaaaaagc cgccaaaagc aaaaagaccg tgatgtttgc cggacgggaa tacggcatat
    40501 ccaaacagaa tttgagcgat gtttgggatg attttgaaga cgcgatggaa ctgaaggcgt
    40561 ttccctgcct gtcttcgctg tttctgacca aatggcataa aaatctatat gatagtggat
    40621 taacaaaaac cagtacagcg ttg
//
LOCUS       DAXHTR010000002        38711 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.002, whole genome shotgun sequence.
ACCESSION   DAXHTR010000002 DAXHTR010000000
VERSION     DAXHTR010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 38711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38711
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.002"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            294..938
                     /gene="rplC"
                     /locus_tag="ACFNML_000046"
     CDS             294..938
                     /gene="rplC"
                     /locus_tag="ACFNML_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFC6391513.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGTSKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            938..1558
                     /gene="rplD"
                     /locus_tag="ACFNML_000047"
     CDS             938..1558
                     /gene="rplD"
                     /locus_tag="ACFNML_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFC6391514.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            1555..1869
                     /gene="rplW"
                     /locus_tag="ACFNML_000048"
     CDS             1555..1869
                     /gene="rplW"
                     /locus_tag="ACFNML_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFC6391515.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLANATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRSDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            1875..2708
                     /gene="rplB"
                     /locus_tag="ACFNML_000049"
     CDS             1875..2708
                     /gene="rplB"
                     /locus_tag="ACFNML_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFC6391516.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQVGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            2715..2993
                     /gene="rpsS"
                     /locus_tag="ACFNML_000050"
     CDS             2715..2993
                     /gene="rpsS"
                     /locus_tag="ACFNML_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFC6391517.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            3002..3331
                     /gene="rplV"
                     /locus_tag="ACFNML_000051"
     CDS             3002..3331
                     /gene="rplV"
                     /locus_tag="ACFNML_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFC6391518.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            3341..4033
                     /gene="rpsC"
                     /locus_tag="ACFNML_000052"
     CDS             3341..4033
                     /gene="rpsC"
                     /locus_tag="ACFNML_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFC6391519.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            4017..4433
                     /gene="rplP"
                     /locus_tag="ACFNML_000053"
     CDS             4017..4433
                     /gene="rplP"
                     /locus_tag="ACFNML_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFC6391520.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            4433..4624
                     /gene="rpmC"
                     /locus_tag="ACFNML_000054"
     CDS             4433..4624
                     /gene="rpmC"
                     /locus_tag="ACFNML_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFC6391521.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            4624..4887
                     /gene="rpsQ"
                     /locus_tag="ACFNML_000055"
     CDS             4624..4887
                     /gene="rpsQ"
                     /locus_tag="ACFNML_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFC6391522.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            5109..5477
                     /gene="rplN"
                     /locus_tag="ACFNML_000056"
     CDS             5109..5477
                     /gene="rplN"
                     /locus_tag="ACFNML_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFC6391523.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            5489..5812
                     /gene="rplX"
                     /locus_tag="ACFNML_000057"
     CDS             5489..5812
                     /gene="rplX"
                     /locus_tag="ACFNML_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFC6391524.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            5822..6361
                     /gene="rplE"
                     /locus_tag="ACFNML_000058"
     CDS             5822..6361
                     /gene="rplE"
                     /locus_tag="ACFNML_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFC6391525.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            6364..6669
                     /gene="rpsN"
                     /locus_tag="ACFNML_000059"
     CDS             6364..6669
                     /gene="rpsN"
                     /locus_tag="ACFNML_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFC6391526.1"
                     /translation="MAKKALINRNLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            6685..7077
                     /gene="rpsH"
                     /locus_tag="ACFNML_000060"
     CDS             6685..7077
                     /gene="rpsH"
                     /locus_tag="ACFNML_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFC6391527.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYADRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            7091..7624
                     /gene="rplF"
                     /locus_tag="ACFNML_000061"
     CDS             7091..7624
                     /gene="rplF"
                     /locus_tag="ACFNML_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFC6391528.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            7638..7991
                     /gene="rplR"
                     /locus_tag="ACFNML_000062"
     CDS             7638..7991
                     /gene="rplR"
                     /locus_tag="ACFNML_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFC6391529.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            8010..8528
                     /gene="rpsE"
                     /locus_tag="ACFNML_000063"
     CDS             8010..8528
                     /gene="rpsE"
                     /locus_tag="ACFNML_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFC6391530.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            8521..8706
                     /gene="rpmD"
                     /locus_tag="ACFNML_000064"
     CDS             8521..8706
                     /gene="rpmD"
                     /locus_tag="ACFNML_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFC6391531.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACVRGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            8708..9142
                     /gene="rplO"
                     /locus_tag="ACFNML_000065"
     CDS             8708..9142
                     /gene="rplO"
                     /locus_tag="ACFNML_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFC6391532.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            9154..10464
                     /gene="secY"
                     /locus_tag="ACFNML_000066"
     CDS             9154..10464
                     /gene="secY"
                     /locus_tag="ACFNML_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFC6391533.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRSEMKSFSRK"
     gene            10469..10687
                     /gene="infA"
                     /locus_tag="ACFNML_000067"
     CDS             10469..10687
                     /gene="infA"
                     /locus_tag="ACFNML_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFC6391534.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            10708..10821
                     /gene="rpmJ"
                     /locus_tag="ACFNML_000068"
     CDS             10708..10821
                     /gene="rpmJ"
                     /locus_tag="ACFNML_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFC6391535.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            10887..11249
                     /gene="rpsM"
                     /locus_tag="ACFNML_000069"
     CDS             10887..11249
                     /gene="rpsM"
                     /locus_tag="ACFNML_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFC6391536.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            11269..11664
                     /gene="rpsK"
                     /locus_tag="ACFNML_000070"
     CDS             11269..11664
                     /gene="rpsK"
                     /locus_tag="ACFNML_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFC6391537.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            11684..12304
                     /gene="rpsD"
                     /locus_tag="ACFNML_000071"
     CDS             11684..12304
                     /gene="rpsD"
                     /locus_tag="ACFNML_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFC6391538.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            12330..13316
                     /gene="rpoA"
                     /locus_tag="ACFNML_000072"
     CDS             12330..13316
                     /gene="rpoA"
                     /locus_tag="ACFNML_000072"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFC6391539.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            13340..13708
                     /gene="rplQ"
                     /locus_tag="ACFNML_000073"
     CDS             13340..13708
                     /gene="rplQ"
                     /locus_tag="ACFNML_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFC6391540.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHETIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            13855..14568
                     /gene="minC"
                     /locus_tag="ACFNML_000074"
     CDS             13855..14568
                     /gene="minC"
                     /locus_tag="ACFNML_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFC6391541.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSDERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQKTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPDHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            14597..15412
                     /gene="minD"
                     /locus_tag="ACFNML_000075"
     CDS             14597..15412
                     /gene="minD"
                     /locus_tag="ACFNML_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFC6391542.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            15416..15679
                     /gene="minE"
                     /locus_tag="ACFNML_000076"
     CDS             15416..15679
                     /gene="minE"
                     /locus_tag="ACFNML_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFC6391543.1"
                     /translation="MSLIELLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVDVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            15683..16603
                     /gene="oxyR"
                     /locus_tag="ACFNML_000077"
     CDS             15683..16603
                     /gene="oxyR"
                     /locus_tag="ACFNML_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HFC6391544.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(16600..16791)
                     /locus_tag="ACFNML_000078"
     CDS             complement(16600..16791)
                     /locus_tag="ACFNML_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391545.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(16933..19770)
                     /locus_tag="ACFNML_000079"
     CDS             complement(16933..19770)
                     /locus_tag="ACFNML_000079"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFC6391546.1"
                     /translation="MLDKYSPAEIESKHYQNWESQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHTFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLVNVFDLEAKVLANAEVFNFKGEAQPSFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDNEG
                     NVYVARNQEEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIDLEKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVGRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESADDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQTQLSKLKD"
     gene            complement(19867..20676)
                     /gene="zupT"
                     /locus_tag="ACFNML_000080"
     CDS             complement(19867..20676)
                     /gene="zupT"
                     /locus_tag="ACFNML_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFC6391547.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTTGMAVIAVSLVLFHF"
     gene            complement(20857..22113)
                     /locus_tag="ACFNML_000081"
     CDS             complement(20857..22113)
                     /locus_tag="ACFNML_000081"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008718 - D-amino-acid dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0055130 - D-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFC6391548.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQAKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIAGGLHLPADATGDCHLFTENLYKLCQEKGVQF
                     HFNQTISRIDHNGLRIKAVETKTGRFEADAVVCALGCFSRTVLAQLDLDLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTHFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(22192..23583)
                     /locus_tag="ACFNML_000082"
     CDS             complement(22192..23583)
                     /locus_tag="ACFNML_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6391549.1"
                     /translation="MQLFLDNLKAFFETVSGWVWGSVMLMLLVGTGIVLTVLLKGLQF
                     TMLGYALKQAFVPSKKYEGGEGHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWITAIFGMATKYGEGVLAVKYRVNNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(23940..24716)
                     /gene="lpxA"
                     /locus_tag="ACFNML_000083"
     CDS             complement(23940..24716)
                     /gene="lpxA"
                     /locus_tag="ACFNML_000083"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFC6391550.1"
                     /translation="MTLIHPTAVIDPKAELDSGVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTKLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTVGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKNVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSTRGIIR"
     gene            complement(24759..25439)
                     /locus_tag="ACFNML_000084"
     CDS             complement(24759..25439)
                     /locus_tag="ACFNML_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HFC6391551.1"
                     /translation="MERYKNAVGEDKAAELYLLNLSLSRELFHVVSIFEIVLRNKIDI
                     CLQQAFKDRNWLYNSIQPQTNPALKYQGCFLRNGTKESAELIKVALSKIQNNSGGKFD
                     HNQLVAGLGFGFWRYLFAGGKDAQFDVTGKVLMKVFPKKPKSTPSVQYNQKWIFRELS
                     NINKFRNRLAHHEPICFKGAIKDTNYARNIHQSIFELLNYMDVDTASVFSHFSDQVIA
                     VCDEIDKL"
     gene            complement(25646..26095)
                     /gene="fabZ"
                     /locus_tag="ACFNML_000085"
     CDS             complement(25646..26095)
                     /gene="fabZ"
                     /locus_tag="ACFNML_000085"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFC6391552.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAIIMCAKRVV"
     gene            complement(26129..27175)
                     /gene="lpxD"
                     /locus_tag="ACFNML_000086"
     CDS             complement(26129..27175)
                     /gene="lpxD"
                     /locus_tag="ACFNML_000086"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFC6391553.1"
                     /translation="MIPATYTLSQITARLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPGATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCRLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(27208..27708)
                     /locus_tag="ACFNML_000087"
     CDS             complement(27208..27708)
                     /locus_tag="ACFNML_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HFC6391554.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKVQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(27774..30167)
                     /gene="bamA"
                     /locus_tag="ACFNML_000088"
     CDS             complement(27774..30167)
                     /gene="bamA"
                     /locus_tag="ACFNML_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFC6391555.1"
                     /translation="MKLKQIASALMVLGISPLALADFTIQDIRVEGLQRTEPSTVFNY
                     LPVKVGDTYNDTHGSAIIKSLYATGFFDDVRVETADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGGRFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKQYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(30224..31564)
                     /gene="rseP"
                     /locus_tag="ACFNML_000089"
     CDS             complement(30224..31564)
                     /gene="rseP"
                     /locus_tag="ACFNML_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HFC6391556.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAAGTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPIASWQEWANLTRQSPGKKITLTYERAGQTHT
                     ADIRPDTVEQPDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVIRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAVAFFNDVTRLLG"
     gene            complement(31599..32834)
                     /gene="ispC"
                     /locus_tag="ACFNML_000090"
     CDS             complement(31599..32834)
                     /gene="ispC"
                     /locus_tag="ACFNML_000090"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFC6391557.1"
                     /translation="MPSENASDGIRYKVILIMTPQVLTILGSTGSIGESTLDVVSRHP
                     EKFRVFALAGHKQVEKLAAQCQTFRPEYAVVADAEHAARLEALLKRDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPA
                     QAVKHPNWSMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLSQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(32839..33636)
                     /locus_tag="ACFNML_000091"
     CDS             complement(32839..33636)
                     /locus_tag="ACFNML_000091"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFC6391558.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(33639..34385)
                     /locus_tag="ACFNML_000092"
     CDS             complement(33639..34385)
                     /locus_tag="ACFNML_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HFC6391559.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFNRQILQGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAEGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(34441..34998)
                     /gene="frr"
                     /locus_tag="ACFNML_000093"
     CDS             complement(34441..34998)
                     /gene="frr"
                     /locus_tag="ACFNML_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFC6391560.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAAKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(35178..36305)
                     /locus_tag="ACFNML_000094"
     CDS             complement(35178..36305)
                     /locus_tag="ACFNML_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFC6391561.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRHRRLIHAVRLGGAVLFATALARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEQNMVKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(36379..36454)
                     /locus_tag="ACFNML_000095"
     tRNA            complement(36379..36454)
                     /locus_tag="ACFNML_000095"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36419..36421),aa:Thr,seq:cgt)
     gene            36561..37033
                     /locus_tag="ACFNML_000096"
                     /pseudo
     CDS             36561..37033
                     /locus_tag="ACFNML_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            37129..37752
                     /gene="rsmG"
                     /locus_tag="ACFNML_000097"
     CDS             37129..37752
                     /gene="rsmG"
                     /locus_tag="ACFNML_000097"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFC6391562.1"
                     /translation="MERKERLQSGIAAMGLDISETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPHIEGVQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVVTSRAFAELADFVSWTVHLLKDG
                     GYWAAMKGVYPQGEIDHLPQDVCVEKVQRLDVPGLDAERHIVILRKR"
     gene            37851..38624
                     /locus_tag="ACFNML_000098"
     CDS             37851..38624
                     /locus_tag="ACFNML_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFC6391563.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVLLGDADVQSAAVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELAARVSGK"
BASE COUNT         9087 a         8743 c        10655 g        10226 t
ORIGIN      
        1 tttgacgaac tgagcatccc cgattacagt catcaaccat attccggttt gttggagaaa
       61 gatgcatacg ctgtgatgac cggataccga ccctttaaaa gagtctgacc ctatgccgtc
      121 tgaaaattca aaacgcttca gacggcatat tgaagatatt tctgatattt ctgttgatat
      181 ttctttaact tgtcagatat aatgccgagc ttggtacatt tgtgccaagt ttaactttgt
      241 ctgaaagaca ggccaatcgt agcctgtccc tttactttaa aaggaaaata atcatgactt
      301 taggtctggt tggacgcaaa gttggtatga cccgcgtgtt cgacgaacag ggtgtttctg
      361 ttccggtaac cgttttggat atgtctgcca accgcgttac acaagtaaaa tccaaagata
      421 ctgacggcta tactgccgtt caagttacct ttggtcagaa aaaagccaat cgtgtcaaca
      481 aagccgaagc cgggcacttt gcaaaagcag gtgttgaagc cggtcgcggt ttgattgagt
      541 ttgctttgac tgaagaaaaa ctggctgaat tgaaagctgg tgacgaaatc accgtttcta
      601 tgtttgaagt cggtcaactg gtcgatgtaa ccggtacctc taaaggtaaa ggtttctccg
      661 gcacgattaa acgtcataac ttcggcgccc aacgtacttc ccacggtaac tcccgctctc
      721 accgcgttcc aggttctatc ggtatggcac aagacccggg tcgcgtgttc cccggtaagc
      781 gcatggccgg ccaatacggc aacaccaagg caactgttca aaaattggaa gttgtccgtg
      841 ttgatgcaga acgccaactg ctgttggtta agggtgctgt tccgggtgcg gtcaacagcg
      901 atgttgtagt tcgtcccagc gtgaaagtag gtgcgtaatg gaattgaaag taattgacgc
      961 taaaggacaa gtttcaggca gcctgtctgt ttctgatgct ttgttcgccc gcgaatacaa
     1021 tgaagcgttg gttcatcagc tggtaaatgc ctacttggca aacgcccgct ccggtaaccg
     1081 cgctcaaaaa acccgtgccg aagtaaaaca ctcaaccaaa aaaccatggc gtcaaaaagg
     1141 taccggccgt gcccgttccg gtatgacttc ttctccgctg tggcgtaaag gtggtcgcgc
     1201 gttcccgaac aaacccgacg aaaacttcac tcaaaaagta aaccgcaaaa tgtaccgtgc
     1261 cggtatggcg actattctgt cccaattgac tcgtgacgag cgtttgtttg cgattgaggc
     1321 gttgactgcc gaaactccta aaaccaaagt ttttgctgaa caagtgaaaa atctgggtct
     1381 agagcaagtg ttgtttgtaa ccaaacagct cgacgagaat gtttacttgg cttcacgcaa
     1441 cttgccaaac gtgttggttt tggaagctca acaagttgat ccttacagct tgctgcgtta
     1501 caaaaaagta atcatcacta aagatgcagt tgcacaatta gaggagcaat gggtatgaat
     1561 caacaacgtt tgactcaagt gattttggca cctatcgttt ctgaaaaaag caacgtattg
     1621 gctgaaaaac gcaaccaaat gacgtttaaa gttttggcaa atgcaaccaa acctgaaatc
     1681 aaagcggctg ttgagctgct gtttggtgtt caagttgctt ctgtaactac cgttacaact
     1741 aaaggcaaaa ctaagcgttt tggtcgtact ttgggccgcc gcagcgatgt taaaaaagct
     1801 tatgtaagct tggctgccgg tcaagagttg gatttggaag ccgctgctgc agctgcagat
     1861 aaggaataaa caaaatggca atcgttaaaa tgaagccgac ctctgcaggc cgtcgcggca
     1921 tggttcgcgt ggtaacagaa ggtttgcaca aaggtgcgcc ttatgcacct ttgctcgaaa
     1981 agaaaaattc tactgccggt cgtaacagta atggtcatat caccacccgt cacaaaggcg
     2041 gcggtcataa acaccattac cgtgttgtag actttaaacg taacaaagac ggcattcctg
     2101 ctaaggtaga gcgtatcgaa tacgatccta accgtactgc cttcatcgca ctgttgtgct
     2161 atgcagacgg cgagcgtcgt tacatcatcg ctcctcgcgg tattcaagtc ggtgccgtat
     2221 tggtttccgg tgctgaagct gccatcaaag taggcaacac cctgccgatc cgcaacattc
     2281 ccgttggtac gactatccac tgtatcgaaa tgaaacccgg taaaggtgct caaatcgcac
     2341 gttctgccgg tgcttctgcg gtattgctgg ctaaagaagg cgcgtacgct caagtccgtc
     2401 tgcgctctgg cgaagttcgt aaaatcaacg taaattgccg tgcgaccatc ggtgaagttg
     2461 gtaacgaaga gcaaagcctg aaaaaaatcg gtaaagccgg tgccaaccgt tggcgcggta
     2521 ttcgtccgac cgtacgcggt gttgtcatga atcctgtcga tcacccgcat ggtggtggtg
     2581 aaggccgtac gggcgaggcc cgcgaaccgg tcagcccatg gggtactcct gctaaaggct
     2641 accgcactcg taataacaaa cgcacggata acatgattgt tcgtcgccgt tactcaaata
     2701 aaggttaatt tagtatggct cgttcattga aaaaaggccc atatgtagac ctgcatttgc
     2761 tgaaaaaagt agatgctgct cgcgcaagca acgacaaacg cccgattaaa acctggtctc
     2821 gtcgttctac cattctgcct gattttatcg gtctgaccat tgctgtgcac aacggccgca
     2881 cccatgtgcc tgtgtttatc agcgacaata tggttggtca taaattaggc gaattctcat
     2941 tgacccgtac ctttaaaggc cacttggccg ataaaaaggc taaaaagaaa taaggtgaat
     3001 catgagagta aatgcacaac ataaaaatgc ccgtatctct gctcaaaagg ctcgtttggt
     3061 agctgatttg attcgtggta aagacgttgc ccaagctttg aatattttgg ctttcagtcc
     3121 taaaaaaggt gccgagctga ttaaaaaagt attggagtca gctattgcta atgccgagca
     3181 caataacggt gcggacattg atgaactgaa agtggtaact atctttgttg acaaaggccc
     3241 aagcttgaaa cgttttcaag ctcgcgccaa aggtcgcggt aaccgcatcg aaaaacaaac
     3301 ttgtcatatc aatgtgacag tgggtaacta aggaaaagct atgggacaaa agattaaccc
     3361 tacaggcttt cgcctggcgg taactaaaga ctgggcttca aaatggtttg ctaaaagcac
     3421 cgacttttct actgttttga agcaggatat cgatgttcgc aattatttgc gtcaaaaatt
     3481 ggccaatgct tcggttggtc gagtggttat tgaacgccct gcaaaatctg cacgcattac
     3541 cattcactcc gctcgtccgg gtgtggttat cggtaaaaaa ggtgaggata tcgaggtttt
     3601 gaaacgtgac ttgcaagtct tgatgggtgt acctgttcat gtaaatattg aagagattcg
     3661 ccgtcctgag ttggatgctc aaattattgc tgacggtatt gcccagcagt tggaaaagcg
     3721 cgttcaattc cgtcgtgcta tgaaacgagc aatgcaaaat gcaatgcgtt ctggtgctaa
     3781 aggcattaag attatgactt caggccgtct gaatggtgcg gatattgccc gtagcgaatg
     3841 gtatcgtgaa ggtcgtgtgc cactgcatac tttacgtgca aatgtagatt atgcaaccag
     3901 cgaagcgcac accacatatg gtgtattggg tctgaaagtt tgggtttata cggaaggcaa
     3961 tattaaatct tccaaacctg aacatgagag taaacaaaga aaggcaggta gacgtaatgc
     4021 tgcagccaac tagactgaaa taccgtaagc aacaaaaggg tcgcaatact ggcatcgcta
     4081 ctcgcggtaa taaggtaagt ttcggtgagt tcggcttgaa agccgtaggt cgtggtcgtt
     4141 tgactgcccg tcaaatcgaa gctgctcgtc gtgcaatgac ccgtcatatc aaacgtggtg
     4201 gtcgtatttg gattcgtgta ttccctgata aaccgattac tgaaaagcct attcaagttc
     4261 gtatgggtgg cggtaaaggt aacgtggaat attacattgc cgaaattaaa ccaggtaaag
     4321 tgttgtatga aatggatggc gttccagagg aactggctcg tgaagcattc gagttggctg
     4381 ctgccaaatt gcctattcct acaacctttg tagtaagaca ggtgggtcaa taatgaaagc
     4441 aaatgaattg aaagacaaat ccgttgagca gttgaatgca gatttgttgg acttgttgaa
     4501 agctcagttt ggcttacgta tgcaaaacgc taccggtcaa ttaggcaaac caagtgaatt
     4561 gaaacgtgta cgtcgcgata ttgctcgtat taaaaccgtt ttaactgaaa aaggtgctaa
     4621 gtaatgagcg aaactaaaaa tgttcgtact ttgcaaggca aagtagtaag cgacaaaatg
     4681 gataaaaccg taacagtatt ggttgagcgt aaagtaaaac atccgctgta tggtaagatt
     4741 attcggttat ctactaaaat ccatgcccat gatgaaaata atcaatatgg aattggtgat
     4801 gtggttgtta tatcggaatc ccgtccattg tcaaaaacta aatcttgggt tgtcagtgag
     4861 ctggttgaga aagcacgttc tatttaagaa ttaaagcaac gtgcttggaa tgggaaacga
     4921 agtattgcag caaatttaat ttgcgtgtaa acttcgtttc ctgtctttca gtttcttctg
     4981 gaagtttctt ccctttcggg gtccaagact ggtttacttg aaccgcaagg tttcatttaa
     5041 taagcagcgg ctttgctgta agttatctga aagtggtaaa ttaagttggt taatttaaag
     5101 gtaataacat gattcaaatg cagaccatct tagatgtggc tgataactct ggtgcgcgtc
     5161 gcgtaatgtg tatcaaggta ttgggcggat ctaagcgtcg ctacgcttct gttggcgata
     5221 ttattaaagt ggcagttaaa gatgcggctc cgcgtggccg tgtcaaaaaa ggcgatgtat
     5281 ataatgcggt agttgttcgt actgctaagg gtgtacgtcg tcctgatggt gcgttaatta
     5341 aattcgataa caatgccgcc gtgttactga ataataaact tgaacctttg ggtactcgta
     5401 tctttggtcc ggtaacccgt gaattgcgta ctgagcgatt tatgaaaatc gtttcattgg
     5461 cacctgaagt attataagga atggcacgat gaataaaatc attaaaggcg atagggttgt
     5521 agtaattgct ggtaaggata aaggtaagca gggtcaagta gttcgagtgt tgggtgataa
     5581 agttgttgtt gagggcgtta atgttgtaaa acgccatcaa aaacctaatc caatgcgtgg
     5641 cattgagggc ggtattatta ctaaagaaat gcctttggat atttctaata tcgcaatcct
     5701 gaatccggaa actaataaag cggaccgtgt tggtattaag ctgattgaaa atgaaggcaa
     5761 agttaaacgc gttcgtttct tcaaatcaaa tggctctatc attggggcat aaggagataa
     5821 catggctcgg ttgagagagt tttataaaga gacagttgtt cctgaattgg ttaaacaatt
     5881 tggttacaaa tcagtaatgg aagtcccgcg tattgaaaaa atcaccttga atatgggtgt
     5941 gggtgaggct gttgctgaca aaaaagttat ggaacatgct gtttccgatt tagagaaaat
     6001 tgccggtcaa aaaccggttg ttactgttgc ccgtaaatct atcgcaggtt ttaaaatccg
     6061 tgataactat ccggttggtt gcaaagtaac attgcgtcgt gatcaaatgt ttgaattctt
     6121 ggatcgtttg attactattg cattacctcg cgtacgtgac ttccgtggtg tgagcggtaa
     6181 atcatttgat ggccgtggca attacaatat gggtgttcgt gagcaaatta tttttccgga
     6241 aattgaatac gataaaattg atgctttgcg tggtttgaat attactatta ctactacagc
     6301 aaaaaccgat gaggaagcga aagctttatt gtcattgttt aaatttccgt tcaaaggata
     6361 atcatggcta agaaagcact tattaatcgt aatctgaaac gtcaagcttt ggctaaaaaa
     6421 tatgcggcta aacgcgcggc aattaaagcg gtaatcaatg attcgaatgc aactgaggaa
     6481 gagcgttttg aggctcgttt gaggtttcaa tccattcctc gtaatgcggc acctgtgcgt
     6541 caacgtcgtc gttgtgcttt gacaggtcgc cctcgtggta ctttccgtaa atttggtttg
     6601 ggtcgtatta aaatccgtga aatcgccatg cgtggcgaaa ttccgggtgt tgttaaagcc
     6661 agctggtaat aggagtaatt aagaatgagt atgcatgatc ctatttccga tatgttgact
     6721 cgtatccgca atgcgcaacg tgctaataaa gcagcggttg caatgccttc ttcaaaatta
     6781 aagtgtgcta ttgcaaaggt attgaaagaa gaaggatata ttgaggactt cgcagtttca
     6841 tctgacgtaa agtctatatt ggaaattcaa ttaaaatact atgcagatcg tcctgtaatt
     6901 gaacaaatca agcgtgtatc tcgccccggt ttgcgtattt ataaagcgtc tagtgagatt
     6961 ccaagtgtta tgaatggctt gggtattgct attgttagta cttctaaagg tgtaatgact
     7021 gatcgtaaag cacgttctca aggtgttggt ggtgagttgt tatgcattgt agcctagtgg
     7081 aggaaaagaa atgtcacgtg tcgcaaaaaa cccagtgact gttcccgctg gtgtagaagt
     7141 aaaatttgga acagaggcat tagttattaa gggtaagaac ggtgaattgt cttttccttt
     7201 gcattctgat gtagccattg aatttaatga tggcaaattg gcttttgttg cgaataacag
     7261 cagtaaacaa gcaaatgcaa tgtctggtac tgctcgcgca ttagtcagca atatggttaa
     7321 aggtgtttca gaaggttttg agaaaagatt gcaattgata ggtgtgggtt atcgtgctca
     7381 agcacaaggt aaaatcttga atctgtcttt gggtttttct catccgatcg tatatgaaat
     7441 gcctgaaggt gtctccgttc aaactcctag ccaaacagag attgttttaa ccggctcgga
     7501 taaacaagtt gttggtcaag ttgctgctga gattcgtgcg ttccgtgctc ctgagcctta
     7561 taaaggtaaa ggtgttcgct atgtaggaga agtagtggta atgaaagaag ccaagaaaaa
     7621 ataattgagg ttcactaatg gataaacata caacccgact ccgtcgtgca cgcaaaaccc
     7681 gtgctcgtat tgcggacttg aaaatggtaa gattatgtgt gttccgaagc aataatcata
     7741 tttatgctca agtaattagt gctgaaggtg ataaagtatt ggctcaagcc tctacattgg
     7801 aagctgaggt gcgcggtagt ctgaaatctg gaagcaatgt tgaagcagct gcaatagttg
     7861 gtaaacgtat cgctgagaag gctaaagcag caggtgtaga aaaggttgct tttgatcgtt
     7921 caggtttcca atatcacggt cgtgtgaagg ctttggctga agctgctcgt gaaaatggtt
     7981 taagcttcta aatatttgga gactttcaga tggcaaaaca tgaaattgaa gaacgcggtg
     8041 acggtctgat tgaaaagatg gtcgcagtta accgtgtaac caaagtagtt aaaggtggtc
     8101 gcattatggc tttctctgcg ctaactgttg ttggtgatgg agatggtcgt attggtatgg
     8161 gtaaaggtaa gtcaaaagaa gtgcctgttg cagtccaaaa ggcgatggat caagcacgac
     8221 gctctatgat taaggtacca ttaaaaaatg gtacgattca tcatgaggtt attggtcgac
     8281 atggtgctac taaagtattt atgcagcctg ctaaagaggg tagtggcgta aaagccggtg
     8341 gacctatgcg tttggttttt gatgctatgg gcattcataa tatctccgcc aaagtgcacg
     8401 gatctactaa cccatataat atcgtacgtg caacattaga tggtttgtct aagttgcata
     8461 ctcctgctga tatcgcagcc aaacgtggct tgacagtgga agacattttg ggagttaacc
     8521 atggctgaac aaaaaaagat tagggttaca ttggttaaaa gcctgattgg tacaattgaa
     8581 tctcatcgtg catgtgtacg cggtttaggt ttgcgtcgtc gcgagcatac ggtagaggtt
     8641 ttagataccc ctgaaaaccg tggtatgatt aataaaatca gctacttgtt gaaagtggag
     8701 tcttgatatg tttttgaata caattcaacc tgctgttggt gctacgcatg ctggtcgtcg
     8761 tgttggacgc ggtattggta gtggtcttgg caaaacgggt ggtcgtggtc ataaaggtca
     8821 aaagagccgg tctggtgggt ttcataaggt gggtttcgag ggtggtcaaa tgcccttgca
     8881 acgacgcctc cctaaaagag gttttaaatc tttaacagca tcagctaatg cacagcttcg
     8941 tttaagtgaa ctggaatcaa ttgctgttaa tgagattgat attttggtct taaagcaagc
     9001 gggtctgatt gcatctacag tctctaatgt taaagttatt gcttctggtg aaatttctaa
     9061 ggcagttgct ttgaagggta ttaaagttac caaaggtgcg agagctgcta tcgaggctgt
     9121 tggtggtaag attgaaatgt aaggtttaat attgtggcta atcaacaaac gtcatcaggt
     9181 tcatccaaat ttggagatct taagaaacgt cttttgtttc tatttggagc attgattgtt
     9241 tttcgaattg gtgcccatat acccgtacct ggagttgatg ctgttgcttt agctaaatta
     9301 tacgaaagcg ctggaaacgg catcctggga atattgaata tgttttccgg tgggtcgtta
     9361 gagcgcttta gtatatttgc aataggaatt atgccatata tttcagcttc tattattgta
     9421 cagctcgctt ctgaaatttt gccatcattg aaggctttaa aaaaagaagg ggaggctggt
     9481 agaaaggtaa ttacgaaata tactaggtat ggtactgttt tgttagcaat tcttcaaagt
     9541 ctaggtgttg catctttcgt atttcagcaa ggaattgttg taacaagttc atttgagttt
     9601 catgtttcca cggtagtttc tttggtaacg ggaaccatgt ttcttatgtg gcttggggag
     9661 caaattactg aaaggggtat cgggaacggt atttctttaa tcattacggc aggtattgct
     9721 tcaggtattc cttcgggtat tgcaaagctg gttacactga cgaaccaagg ttctatgagc
     9781 atgcttacgg cgttgtttat tgtatttggt gccttattat taatttattt ggttgtatac
     9841 tttgaaagtg cacagcggaa gattcctatt cattatgcaa aacgccagtt taatggtagg
     9901 gcgggtagtc aaaatacgca tatgcctttc aagttgaata tggctggtgt tattccccca
     9961 atttttgctt ccagtattat tctatttcca tctactcttt taggttggtt tggttcggct
    10021 gatacaaata gtgttttgca caaaatagct ggattgttac aacacggtca attgctgtat
    10081 atggctttat ttgcagcgac agttattttc ttttgttatt tttatacggc tttggttttt
    10141 agccctaaag aaatggcaga gaatttaaaa aagagtggtg cttttgttcc tgggattaga
    10201 cctggtgagc agacctctag gtatttagaa aaagttgtat tacgtttgac attgtttgga
    10261 gctctttata ttacaactat ttgtttaatt ccagagttct taactacggt tttaaatgta
    10321 cctttttatt tgggtggcac gtctttgttg attctagttg ttgtaacgat ggattttagt
    10381 acacaaataa attcgtatag gcttactcaa cagtatgata agttaatgac tcgttcagaa
    10441 atgaaatcat tttctcggaa atagaattat ggcgaaagaa gatactatcc aaatgcaagg
    10501 tgaaattctt gaaactttac ctaatgcaac atttaaagta aaacttgaga atgaccatat
    10561 tgtattgggt catatttctg ggaagatgcg gatgcattac attcgtattt ctccgggaga
    10621 taaggtcaca gtagagctga caccttatga tctaactagg gctcgaatcg ttttcagagc
    10681 aagataaacc aataaaagga aaataaaatg cgtgtacaac catctgttaa gaaaatttgc
    10741 cgaaattgca agattattcg tcgaaatcgt gtagttcgtg taatttgtac tgatctccgt
    10801 cacaaacagc gtcaaggtta atggaatatt tcttttaatg tgattctgtg atatagtgac
    10861 acactttgcc ctaaaaagga aaaaatatgg ctcgtattgc aggggtaaat atccctaata
    10921 acgcacacat cgtaattggt cttcaggcta tttacggtat tggtgctact cgtgctaaat
    10981 tgatttgtga ggctgcaaat attgcgcctg atactaaagc aaaagatttg gacgagactc
    11041 aattagatgc tttgcgtgac caagttgcca agtatgaagt agaaggtgat ttgcgtcgtg
    11101 aggtaactat gagtatcaag cgattgatgg acatgggctg ctatcgtggc ttccgtcatc
    11161 gtcgcggctt accatgccgc ggtcaacgca ctcgtacaaa tgcgcgtacc cgcaaaggtc
    11221 cgcgtaaagc gattgctggt aagaaataaa ttttaaggaa ttttattaat ggctaaagca
    11281 aacacagctt cacgtgtacg taaaaaagta cgtaaaaccg tgagtgaggg tattgtgcac
    11341 gttcatgcat ctttcaacaa taccatcatt acaatcactg accgtcaagg caatgcgttg
    11401 tcttgggcta cctctggcgg cgctggtttt aaaggttctc gtaaaagtac accatttgca
    11461 gcacaagttg cagcagaagc agctggtaaa gttgcccaag agtatggcgt taaaaattta
    11521 gaggttcgta ttaaaggtcc aggtccaggt cgtgaatcct ctgtacgtgc tttgaatgct
    11581 cttggtttca agattaccag cattactgac gttaccccgt tgcctcataa cggttgccgt
    11641 ccgcctaaaa aacgtcgtat ttaatattgg agtgatttga aacatggcac gttatattgg
    11701 ccctaaatgt aagttggcac gtcgcgaagg tacggatttg tttttgaaga gtgcgcgccg
    11761 ctctttggat tctaaatgta aaattgattc cgctcccggt cagcatggtg caaaaaaacc
    11821 gcgtttgtca gactatggtt tgcagttgcg tgaaaaacaa aaaatccgcc gtatttatgg
    11881 cgtattagaa cgtcagttcc gtcgttattt cgcagaagct gatcgtcgta aaggttctac
    11941 cggcgagttg ctgttgcagt tgctggaatc tcgtttggat aatgtcgttt atcgtatggg
    12001 tttcggttct acccgagctg aagcaagaca gcttgtttct cataaggcga tagttgtgaa
    12061 tggacaagtt gtcaatattc cttctttcca agtgaaagct ggtgatgttg tctcagttcg
    12121 tgaaaaagcc aaaaaacagg tacgtattca agaagcattg ggtttggcaa ctcaaatcgg
    12181 cttgccgggt tgggtttctg tagatgcgga taaacttgag ggtgtgttca aaaacatgcc
    12241 ggatcgctcg gaattgaccg gtgatattaa tgaacagctg gtggtagagt tctactctaa
    12301 ataatgctag ctcagtgagg gacagttaaa tgcagaatag cacaaccgaa tttttgaaac
    12361 ctcgtcaaat tgatgtaaat actttttctg caactcgtgc aaaagtatct atgcagccat
    12421 ttgaacgtgg tttcggtcat accttaggta atgctttgcg ccgtatctta ctgtcatcca
    12481 tgaatggttt tgctcctact gaagtagcta ttgccggtgt attacacgaa tattctactg
    12541 ttgatggtat tcaggaagat gttgttgaca ttttgctgaa tattaaaggt attgtgttta
    12601 aactccatgg tcgtagccaa gttcaacttg tgttgaagaa atcaggttca ggtgtcgtat
    12661 ctgccggtga tattgagttg ccgcatgatg tagaaattct gaatcctggt catgtcattt
    12721 gtcatttggc tgataacggt caaattgaga tggaaattaa agtagagcaa ggtcgtggtt
    12781 atcaatctgt ttcaggtcgt caggtagttc gtgatgagaa ccgtcagatt ggtgcaatcc
    12841 agttggatgc gagcttttcg cccatcagcc gtgttagctt tgaggttgaa cctgcacgtg
    12901 tagagcagcg gacggatctt gataagttgg ttttggatat cgaaaccgac ggttctattg
    12961 atcctgagga agctgtacgc agtgcggcac gtattttgat tgatcagatg tctatttttg
    13021 ctgatttgca gggtacgcct gtggaggagg ttgaagaaaa agcacctcct atcgaccctg
    13081 ttcttttgcg tccggtggat gatctggaat tgacagtacg ttcagctaat tgtttgaaag
    13141 ctgaggatat ttattatatt ggcgatttga ttcaacgcac tgaaaccgag cttcttaaaa
    13201 cgccgaattt gggacgtaaa tctttgaatg agattaagga agtattggca tctaaaggtt
    13261 tgacactggg ttctaagttg gaagcatggc cacctgtagg cttggaaaag ccttaatgaa
    13321 gaattaaagg ataattgata tgcgtcatcg taatggcaat cgcaaattaa accgtaccag
    13381 cagtcatcgt gctgcaatgc tgcgtaatat ggcgaattca ttattgactc acgaaactat
    13441 tgtaacaact ctgcctaagg ccaaggaatt gcgccgtgta gtagagccgt tgattacatt
    13501 gggtaaaaag ccgtcattgg caaaccgccg tttggcattt gaccgtactc gcgaccgtga
    13561 tgttgtagta aaactgtttg gcgatttggg tcctcgtttt actgctcgta acggtggtta
    13621 tgttcgggtg ttgaaatacg gattccgtaa aggtgataat gcacctctgg cactggttga
    13681 attggttgac aaaccggctg ctgagtaatt ttagtcatat aacgccatct gccgaaaagc
    13741 aggtggcgtt atttttgcaa tatctgatag gtaatagggt attggctatc atgtttaaaa
    13801 tattaattga atagctaagg tttgcgcggt aaacttacat cattaaaaaa ttctatgatg
    13861 gtttatataa tgaatgcctt cgatataaag tcgacaaaga tggacgtatt gtctatatct
    13921 ttgcatacat cagacttgtt tgatttggaa gatgtgctgg tcaaattggg caagaagttt
    13981 caagagtctg gtgttgttcc atttgttctg gatgttcaag agtttgatta tcccgagtct
    14041 ttggatcttg ctgcattggt ttcgttgttt tcaaggcatg gtatgcaaat tttgggtctg
    14101 aagcattctg atgaacgttg ggctgctgcg gctatgaagt atcatttgct gttttgtctg
    14161 tctcattcgg aaaatgttaa agaactgggt caggttgagg tgcagaaaac ggaggatggt
    14221 cagaaagcaa ggaaaacagt attgattaca tcccctgtcc gtaccggtca gcaggtttat
    14281 gccgaagatg gcgatttgat tgttacgggg gcggtcagcc agggggcaga attgattgcg
    14341 gatggcaata tacatattta tgcgccgatg agggggcgtg ctttggccgg cgccaagggt
    14401 gatacttctg cccgcatatt tatccactcc atgcaggcag aactggtttc tgtggcgggt
    14461 atttaccgta attttgaaca ggatttgccg gaccatctgc acaagcagcc ggtacagata
    14521 ttgttgcagg ataaccgatt ggttatcagt gcaattggct cagagtaatt gtttgatatt
    14581 ttaaaaagga aatattgtgg caaaaattat tgtagtaact tcaggtaagg gcggtgtcgg
    14641 taaaacgact accagtgcca gtattgcgac aggtttggca ttacgcggat ataaaactgc
    14701 ggtaattgat tttgatgtgg gtttgcgtaa cctcgacctc attatgggtt gcgagcgtcg
    14761 tgtcgtttat gacctgatca atgtcattca gggggaggcg acgctcaacc aggctttgat
    14821 taaagataaa aattgtgaaa acctgtttat tttgccggct tcccagactc gggataaaga
    14881 cgctttgaca cgcgagggcg tagaaaaagt gatgcaggag ctgtccggca agaaaatggg
    14941 ctttgagtat attatttgcg actctcctgc cggtattgag cagggtgcat tgatggcgtt
    15001 gtattttgct gatgaagcca ttgtaacgac caatcccgag gtttccagtg tgcgtgactc
    15061 cgacaggatt ttgggaattt tgcaaagcaa atcccataag gcagagcaag gcggttcggt
    15121 taaagaacat ctgttgatta cgcgttattc tcccgaacgt gtggcaaaag gcgaaatgct
    15181 gtctgtacag gatatttgcg atattctgca tattcctttg ctgggtgtga ttcccgaatc
    15241 ccaaaacgtc ttgcaggcat ccaattccgg agaaccggtc atccatcagg acagcgtggc
    15301 ggcttccgag gcatataagg acgttattgc ccgtcttttg ggcgagaacc gtgaaatgcg
    15361 tttcttggaa gctgagaaaa aaagcttctt caaacgtctg ttcggaggat gaggtatgtc
    15421 attaatcgaa cttttattcg gcagaaagca gaaaacggca accgttgccc gcgaccgcct
    15481 tcaaatcatc attgcccaag agcgcgccca agaaggtcag gctccggatt acctgccgac
    15541 tttacgtaaa gagttgatgg aagtcctgtc caaatatgtg gatgtttcat tagacaatat
    15601 ccgtatttcc caggaaaaac aggatggtat ggatgtgctt gagttgaaca ttactttgcc
    15661 ggaacagaaa aaggtatagg acatgacctt aaccgaattg cggtacatcg tcgcagtcgc
    15721 ccaagaacgt catttcggca gggcggcgcg gcgttgtttt gtcagccagc ccactttgtc
    15781 tattgccatt aagaaattgg aagaagagct tgccgtctct ttgtttgacc ggagcagcaa
    15841 cgatattatt acgaccgagg cgggggaacg tatcgttgca caggcgcgta aggtgttgga
    15901 agaggcagag cttatcaggc atttggcaaa tgaagaacaa aacgagctgg agggtgcgtt
    15961 caaactcggg ctgattttta cggttgcgcc gtacctgctg ccgaaactga ttgtctcgtt
    16021 gcgccgtact gcaccgaaaa tgcctttgat gttggaagag aattacacgc atactttgac
    16081 cgagtcgctc aaacgcgggg acgttgacgc gattatcgtt gccgaaccgt ttcaagagcc
    16141 gggcattgtt accgaaccct tgtatgacga accgtttttc gtgattgtcc cgaaagggca
    16201 ttcatttgag gaactggatg ccgtttcgcc acggatgctg ggtgaggagc aggttttgct
    16261 gctgacggaa ggcaactgta tgcgggatca ggtactctca agctgttccg aattggcggc
    16321 gaaacaacgc atacaggggt tgaccaatac attgcagggc agctcgatca atacaatccg
    16381 ccatatggtc gccagcggtt tggcaatcag cgtgttaccg gcaaccgcgc tgaccgaaaa
    16441 cgatcatatg ctgttcagca ttattccgtt tgaaggtacg ccgccaagcc ggcgggtcgt
    16501 attggcgtac cgccgcaatt ttgtccgtcc gaaggcgttg tcggcgatga aggcggcgat
    16561 tatgcagtcg cagcttcacg gggtaagttt tatccgcgac taggcgcagg cattgttttc
    16621 aaaacgccat ttccctgagc cgacaacacg gtatgccaag atattgccgt catcatcgat
    16681 tttgagtata gcatcgccac ggaaactgcc gtcctgaaga tattcgactt ttgcatcact
    16741 gtgaatgttt tcatcagtgc cgatgcaatg ccatgtatag tggattaaca aaaaccagta
    16801 cggcgttgcc tcaccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
    16861 atttttgtta atccactata aaagaggccg tctgaaaaac atttttcaga cggccttgtt
    16921 gattcaatca aatcagtctt tcaacttcga caactgagtt tgcacttttg ccattttgtc
    16981 ttccaattcc gccaaatcgg ctttgtcttt ttccaccaaa tgcgccgggg ctttttcggt
    17041 gtagccgggt ttggagagtt tggcgttgag tttgtccaag gctttttgca gcttctcggc
    17101 ttctttgctc aaacgggcgg tttcggcagc tttgtcgatt tcgactttca acatcaggcg
    17161 cgcgccgttg cagacggcga cgggcgcgtc ttcgctttcg ggtagggcgg cgacttgctg
    17221 tgcttcggtc aggcgggtca tcatcggcag gtatttgagg tagtccgcca agtcgtctgc
    17281 gctttcgaca aacagcgggg ctttcacgtt gggctggatg cccatttcgc cgcgcaggtt
    17341 gcggactgcg ccgatcaaat cctgcaacac ggtcatttgc ccgaatgccg tctgaacaat
    17401 ctcgccgccg tcggcttcgg ggaagcgggc gagcatgatg ctgtcggcgg ttttcgcgtc
    17461 gcacataggg gcgacggttt gccacagttc ttcggtgatg aacgggataa tcgggtgcag
    17521 caggcgcagg gcggcttcga gtacgcgcaa taaggtatgg cgtgtggcgc gttggcggct
    17581 ggcgcagccg gtttgaagct gcactttggc aagttccaaa taccagtcgc aatagtcgtt
    17641 ccacacgaag ctgtacaggg tttccgccgc caaatcaaag cggtaggttt cgtaggcttg
    17701 cgtaacctgc tcgatggtct gattcagacg gcctacaatc cacatatcgg ggaaggaata
    17761 gccgcgcggt tcggtggcgg ttgcgccgta gccgcagtct tggttttcgg tgttcatcaa
    17821 gacgaagttg gtggcgttcc agattttgtt gcagaagttg cggtagcctt cggcgcgttt
    17881 gaagtcgaag ttcacgctgc gccccaagct ggcgtagctc gccatggtga aacgcaatgc
    17941 gtccgcgccc atactcggaa tgccttcggg aaagagtttt ttggtggctt cttccacttt
    18001 cggcgcggtt tcgggtttgc gcagaccggt ggtacgtttc accagcagtt tttccaagtc
    18061 gatgccgtcg atcaaatcca cagggtcaat gacgttgcct tcggatttgg acattttttt
    18121 gccttcgtgg tcgcgcacga tgccgtggat gtacacgtct ttaaacggta ctttgccggt
    18181 aaagtgggtg gtcatcataa tcatgcgcgc cacccagaag aagatgattt cgtagccggt
    18241 gaccaagacg ttggacggca ggaaggcttt gagttcgtcg gtttcagacg gccagccgag
    18301 cgtggagaac ggcacgagtg cggaggagaa ccatgtgtcc aatacgtctt cttctcgggt
    18361 caagcctgtt ttgccggctt gtttttcggc ttcttcctga ttgcgggcaa cgtacacatt
    18421 gccttcgttg tcgtaccatg cagggatttg atgcccccac cacagttggc gcgagatgca
    18481 ccagtcttgg atgttgttca tccattggtt gtaggtgttg acccagtttt cggggataaa
    18541 gcgcaccgcg ccgctgtcga cggctttttt ggctttgtca gcaaggctta agcctttgaa
    18601 ttcgctgtca ggctcgccgc cgtttggcgt ggcggacatg gcgacaaacc attggctggt
    18661 cagcatcggc tcaatcacgg aaccggtacg gtcgcctttc ggcgtcatca gcgtgtgcgc
    18721 tttgatttcg accaagaaac cttgttcctg caaatcggca accatttgtt tgcgcgcggc
    18781 aaagcggtcc aagcctgcgt atttttcagg cagggcaaag cttggttgcg cttcgccttt
    18841 gaagttgaac acttcggcgt ttgccaatac tttggcttcc aagtcgaaca cattaaccaa
    18901 gcgcgtatcg tggcgtttgc cgacttcgta gtcgttgaag tcgtgcgcag gcgtgatttt
    18961 cacgcagccg gtgccgaagt ctttttcaac gtattcgtcg gcaatcacgg gaatggtgcg
    19021 gccggtcagc ggcaggatta attctttgcc gattaagtgg gtataacgtt cgtcttcggg
    19081 attgacggca acggcaacgt cgcccaacag cgtttcggga cgggtggtgg cgacgatgac
    19141 ggcttcggca ggattgtccg ccagcggata gcggatgtgc cacatagagc cttgttcttc
    19201 cacgctttcc acttccaaat cagacactgc ggtacccaat accggatccc agttcaccaa
    19261 gcgtttgccg cgataaatca agccctgttc atacaggcgt acgaacactt cggttacggt
    19321 ttcggcgcgt acatcgtcca ttgtgaaata ctcgcgcgtc cagtcggcag aacagcccac
    19381 acggcgcatc tgctgcgtaa tcgtgccgcc ggaaacttct ttccattccc acactttttc
    19441 caagaatttt tcacgaccca agtcatgacg ggacacgttt tgcgcggcaa gctgacgctc
    19501 gaccacgatt tgcgtggcga tgcccgcgtg gtcggtgccg ggaatccagg cggtgttgca
    19561 gcctttcatg cggtagtagc gggtcagacc gtccataatg gtttggttga aggtatggcc
    19621 catgtgcagc gtgccggtta cgttgggcgg cggcagttgg atggaaaaag acggtttggt
    19681 caaatccata tcgggctgga aataaccttg gctttcccag ttttgatagt gtttggattc
    19741 gatttcggcg ggggagtatt tgtctaacat gatggaactt tgtgaaatta aggttatttt
    19801 tgatgtgcgg attataacgc aaaaaggccg tctgaatcat ttcagacggc ctttggcata
    19861 cgggttttaa aaatggaaca ataccaggct gacggcaatc accgccatac ccgttgtcag
    19921 gccgtaaacg gtttcatggc cgtctgaata gcgtttggcg gccggcagca gctcgtccaa
    19981 cgccaaaaac accatcacac cggctatcac gccgaatacc gaaccaaaca cggcaggcga
    20041 caaaaacggc tgcaaaacca aatagcccaa agccgccccc aacggctcgg ccaagccgga
    20101 tagcagacac gcccacaccg ttttcttacg gctgcgggtg gcaaaataaa ccggcgcggc
    20161 gatggaaatg ccctccggaa tattatggat ggcaatcgcc aaggccaaag gcatcccgac
    20221 cgccggattt tccaatgtgg caaaaaacgt cgccaagcct tcggggaaat tgtgcgcagt
    20281 aatcgcaaac gccgccatca tgccgactcg cgcgatatgg cggcgtttgc tttcttgaaa
    20341 cgacgggtct tgcgcgtcta aagtttcatg cgggttcggc accagacggt caatcagcgc
    20401 aatgccgccc atcccggcca aaaatgccat ggtcgccgcc gcaaacgcgt ggtctttatc
    20461 ataaatttca gcgaacgcct cgctggactt actgaaaatc tccgtcaggg aaacatacac
    20521 cattgcaccg ccggcaaatg ccaaaccaaa cgacaacaca cgcggattgg gcgttttgga
    20581 aaacattacc aagccgctgc ctaatacggt aaacaaaccg gcagccaacg tgatggaaaa
    20641 ggcaacggcc aaattggaca tcgaaaaatc gggcatgaga aaacctgcgc taaaagctgg
    20701 gacaggttca gactaacact ttttaatgcg tatgataata gttattattt attttattgg
    20761 ttggatacac ggattttgaa acaaaaggcc gtctgaaaaa tagttttcag acggccttta
    20821 catttgaaat gccactaaac cttatcgctt tccagcttaa gcctgatagc gcgacaggct
    20881 caaatcgtcg ctgcggattt cggtgtcttt gccgctcacg atatcggcgg ttaattttgc
    20941 cgaacccagc gacatggtcc agcctaaagt accgtggccg gtattcagaa acaggttgtc
    21001 aaagtgggtg cgaccgatta acggcgtgct gtcgggcgtc atcggtctga ggccgctcca
    21061 gaacgatgct tggctcaaat cgccgccttc cgggaacaag tcgttgacga ccaaagccaa
    21121 ggtttcgcgg cgtttttcgg gcagtttgat ttcgtagccc gacaattctg ccatgccgcc
    21181 gacgcggatt ctgttgtcaa agcgcgtgat agcgactttg tagctttcat ccaaaacggt
    21241 ggacaccggc gcgccgtctg aattggtgac cggcagggtc aaggaatagc ctttgacggg
    21301 ataaatcggc agatcgaggt ctaactgcgc caacacagtc ctactgaagc agccgagcgc
    21361 acagacaacg gcatctgctt caaaccgccc tgttttggtt tcaacggctt tgatgcgcag
    21421 cccgttatgg tcgatgcggc tgatggtttg gttgaaatga aactgtacgc ccttttcctg
    21481 acacaatttg tataggtttt cggtgaagag gtggcagtcg ccggtcgcat ctgcaggcag
    21541 gtgcaggccg ccggcaattt tggcggtaac gcgtgccagc gcaggctcaa attctgcaca
    21601 ttcttcgggt ttcagacggc ggtacggcac gccgtagcgt tccaaaacgg caatgtcttg
    21661 ttctgccgct tcaacttctt tagcctgacg gaaaatctgc aacgtccctt ttttgcgttc
    21721 ctcaaaattc atgccggttt gcgcttcaaa acggcggaac atttcacggc tatattcgga
    21781 aatcctgacc atgcgctctt tattggtttg atagtgcgct gccgtgcagt tttgcagcat
    21841 ttgccacagc cattcgattt gatacaggct gccgtcgggg cgaaacagca aaggcggatg
    21901 gcttttaaac agccatttca gtgctttggt cggaataccg ggtgcagccc aaggcgtggt
    21961 atagccgtaa gaaagctggc ctgcattggc aaaactggtt tccatcgcca caccctcggc
    22021 gcggtcgatg accgttactt catgtcctgc ctctgccaga taccacgcgg aagacacgcc
    22081 ggcaacaccc gcacctaaaa caagcacttt catgtttctc cctccggctt tttcaaaaca
    22141 gacttaatat gccgtgccgt ctgaatattc ggattcagac ggcctcggat attaatgcgg
    22201 caattcgcca tttgtgattt tttgtttgaa gtcgcgcgtt tcattgacga tgactttcgc
    22261 catcaataaa agtgcaatca ggttgggcaa tgccatcaag ccgttgaatg tgtccgaagc
    22321 cagccacacc aaatcaaggc tcaacacggt acccagcata acggaagaaa cataaccgac
    22381 gcggtacaaa ccggcaaatt tctcgccgaa aacatacacc gcgcattttt cgccgtaata
    22441 gcaccagccg agaatggttg agtaggcaaa gaaaatcagg ccgatggtaa caatccagcc
    22501 gccgatgccg ggcagcattt tttggaatgt gacggttgtc agtgccgcgc cgctcacttc
    22561 aggtttgaca aactcgccgc ccgcgccgag cagtcccatt accaacacga tgccggtaat
    22621 cgagcaaacg acgatggtat ccaaaaacgt accggtcata gaaaccaagg cctgacggac
    22681 gggatggtcg gttttcgcgg ctgcggcggc aataggcgca gaacccatac ccgcctcatt
    22741 ggagaacacg ccgcgcgcca cgccgtagcg gatgaccgta ccgatagcgc cgcccgccac
    22801 tgcctgcgcg ctgaacgcat cggagaaaat cagcttgacg gcaggcatca gtgcatcgga
    22861 attaatcgcg ataatggaaa gaccgcccaa cacataaaac accgccatag caggcacgat
    22921 gaaagaagcg gctttggcga tgcctttaat accacctaaa acgacaacgg cagtcagaac
    22981 ggtcaacgta atgccggtat aggcaggttc gataccgaag ctggtttgca ccgcctgtgc
    23041 aaccgagttg gactgcaccg agctgccgat accgaaggaa gcgaatgtgc cgaacagcgc
    23101 aaatgcaacg gccatccatt tccagttttt gcccaagcct ttttcgatgt aatacatcgg
    23161 gccgccggac atttcgcctt tggaattgtt gacgcggtat ttcaccgcca acacgccctc
    23221 gccgtatttg gtggccatac cgaaaatggc ggttatccac atccaaaata ccgcgcccgg
    23281 gccgccggtt accaccgcag tcgccacgcc ggcgatgttg cccgtaccga tggtggcgga
    23341 cagcgcggtc atcaacgccg caaaatggga aatatcgcct tcgtggcctt cgccgccttc
    23401 atacttcttt gacggcacaa acgcctgttt cagcgcatag cccaacatag tgaactgcaa
    23461 gcctttcagc aaaacggtca gcacaatgcc cgtgccgacc agcagcatca acataacaga
    23521 tccccagacc cagccgctga cggtttcgaa aaaggctttg agattgtcta aaaacaattg
    23581 catggctttc tcctttgtct gttttatttt taaaacacca cttttgtagt gtcctataat
    23641 ttcagcacag aatattcaat aagacaatat gttcttttga aaaatacttt tggttttttc
    23701 gccgaaagca ggtcggttca agctgcggaa attgtttgca attctttaaa agcagcggcg
    23761 gaggtcacaa tgaaatgtcc gaatggggat gtggcgggcg gcagaaatca tcaatgctgc
    23821 cgactgccat acttctgaaa tctacaaaat gatgcatcga tcaaacaata taccgcttta
    23881 aaaaaaccga tgccgtctga aacgctttcg gggcttcaga cggcatcaaa aggatacggt
    23941 cagcggatga tgccgcgcgt cgattgtgcg aaaaagtctc ggaatacggc aagctcggct
    24001 tgggtttcgg cgcggcggag gatgtctgcc ttggcctctt caaacggaat gccgcgatgg
    24061 tagagggttt tgtacacgtt tttgacggcg gaaatctgct ctgcggtaaa accgttgcgg
    24121 cgcatgcctt cgctgttgag ccccgccggt tcggcgcggt agcccgatgc cataaagtag
    24181 ggcggcacgt ctttgtgtac gcctgcggca aacgcggtca tggcgtagtc gccgatgcgg
    24241 cagaattgga aaaccagcgt gtagccgccc aaaacgacgt agtcgccgac ggtaacgtgt
    24301 ccggcaagcg aggcgttgtt ggcgaagatg gtgtggttgc cgaccacgca gtcgtgcgcg
    24361 aggtggcagt acgccataat ccagttgtcg tcgccgatac gggtttcgcc gatgccggtt
    24421 accgtgccta aattaaaggt ggtgaattcg cggatggtgt taccgttgcc gataatcagc
    24481 ttggtcggct cgtcgcggta ttttttgtcc tgcggaattt cgccgaggct ggcaaattgg
    24541 aaaatgcggt tgttttcgcc gatgctggtg tggccgttga tgacggcgtg cggaccgatt
    24601 tcggtattcg caccgatttg gacgttgggg ccgataacgg tgtacgcgcc gactttgacg
    24661 ccggagtcga gttcggcttt ggggtcgatg acggcggtcg ggtggatgag ggtcatgttt
    24721 tgaatactcc gctgcttgtt aagacaaata atagaaaatt acagtttgtc gatttcatcg
    24781 cagaccgcaa tcacttgatc gctaaaatgg ctaaatactg aagcagtatc tacatccatg
    24841 taattaagca gttcaaaaat agattggtga atatttctag cgtagttggt atcttttatt
    24901 gcacctttaa aacaaatcgg ctcgtgatgt gccaaacgat tacggaattt gttaatattg
    24961 gaaagttctc gaaaaatcca tttttggtta tattgcacac ttggtgtaga tttgggcttt
    25021 tttggaaaaa ctttcatcaa tactttccct gtaacatcaa attgggcatc tttccctcct
    25081 gcaaacagat accgccaaaa accgaatcct agtcctgcaa ccaattggtt gtggtcaaac
    25141 tttcccccgc tattattttg tattttagat aatgcaacct tgattaattc agcagattct
    25201 ttcgtgccat ttcttaaaaa gcaaccttga tattttaatg cgggattcgt ctgtggctgt
    25261 atgctgttat ataaccagtt tctgtcttta aacgcctgct gaaggcaaat gtcaattttg
    25321 tttcttaaaa caatctcaaa tattgaaaca acatgaaaaa gttctcttga taaagataaa
    25381 ttcaataaat acaattcagc tgctttgtct tccccaacgg catttttata ccgttccatt
    25441 ctgaaatcag aaaaaatact acagcattcc ttataatcca cgctaacacc tgattcttta
    25501 tttttaaatt gacgtttcaa actatatttg atacagttag gtaaaactaa tccccaccgt
    25561 gtccctgggt ttgaataaac ctaaaccggt gggttttctt tttattaggt cgtctgaaac
    25621 tttcagacga cctttttctg aacactcaaa ccacgcgttt ggcacacatg atgatggctt
    25681 cgacggcgac ttgcccgtcc actttggcaa cggcgttgaa tttgccgatg ccgcgccggc
    25741 tggtcagcag ctcgacttca aagacgagtt ggtcgccggg gatgacttgg cgtttgaagc
    25801 gggcttcgtc tatgccggcg aagaagaaga attcgttttc tttgcgcccg ccctcgctca
    25861 aaatcgccaa cgtgccgcac gcctgcgcca tcgcttcgat gatgagtacg ccgggcatca
    25921 cgggcaggtc gggaaagtgg ccttggaact gcggttcgtt tatggttacg tttttgatgg
    25981 cggtcagggt tttcatcggc tcgaaggcgg tgatgcggtc gagctggaga aacggatagc
    26041 ggtgggggat gagtttttgg atgtctttgg cttcgatggg gagttgtacg tccatgttcg
    26101 tcgtattcct tgaataaagt cggtttggtt atttgctgtc ttgaccggca tctgaaagct
    26161 gctgctccag tgttttgagg cgtttgttca tttcgcttaa gcggtggatg taaacggcgt
    26221 tgcgcgccca ttctttatgg gtggacatcg ggaagatgcc ggcgaggtgt ttgccgcttt
    26281 cggtaatgct gtgggtgacg gacgtgccgc cgccgatggt ggttttgtcg gcgatttcga
    26341 tgtgtccgac cgtaccgacc ccgccgccga tgatgcagta gctgcctatg gttacgctgc
    26401 cggagatgcc ggttttggcg gcgatgacgg tgtgcgaacc gattttgcag ttgtgcccga
    26461 tttggacttg gttgtcgatt ttggtgccgt tgccgacggt ggtgtcgctc atcgcgccgc
    26521 ggtcgatgtt ggtgttcgag ccgatttcta cgtcgtcgcc cagcgttacc gcgccggttt
    26581 gcgggatttt gaaccacgaa tcgccggcaa aggcgagtcc gaaaccgtcc gcgccgatga
    26641 ccgcgccgct gtggatttcg acgcgtctgc cgagtgtgca gccgtaataa acgacggcgt
    26701 tgggatgcag gacgacttcg tcgcccagtc tgcaatcgtg ttggacgacg gcgtttgcca
    26761 agatgcggca gccttcgccg agtacggtgt tcgcgccgat gtaggcgttc gcaccgattt
    26821 cgcagcttgc ggggacggtc gcgcccggtt cgacgacggc ggtcggatgg atgccgccgc
    26881 gcgctttgac gacgggtgaa aacaggcgcg cgactttggc gaaatagaga taggggtcgt
    26941 cggcgacaat caggttgcgc ccttcaaatc cgtctgccgc tttggcggaa acgatgaccg
    27001 cgcccgcgct gctgtcgtgg acttcggctt tgtatttcgg attggcaagg aagctgatgt
    27061 gttccgcctg cgcgtctgcg agcgggcgca cggcggtaac ggaaatgtcc tcgccgcgcc
    27121 attcgccgcc gagccgcgcg gtgatttggg acagggtgta ggtggccgga atcatggttt
    27181 tcctgttcgg tatgccgtct gaaagggtca gcgggcgttc atttctttaa tgacgctgtc
    27241 ggtaacgtcg tattgggtgt tgacgtaaat cacgttctgc aaaatgacat cgtaaccttc
    27301 ctgtttggcg attttgacga tgacgcggtt ggcgttttgc tggagggagg caaactcttc
    27361 gttgcggcgg aggttgtagt cttcttcaaa ctgcgcctgt tttttgcgga acgctgcgac
    27421 cagcccgcgc catttttctt cggcttgcac cttttttgcg tttctgagtt tgccttcggc
    27481 aagctgcctt tccaaatcca gaccttcgcg ttgcagtttt tgcaattcgt cctgacgagc
    27541 ggaaaattcg ctgtccagcg ttttttgaat cttgcgcgcc tgcttggatt cgaggtagat
    27601 gcgctcggtg ttgataaagc cgattttttg gaaggtgtcg gcgtgcgcgc ctgcggtgca
    27661 gcacaaaccg atcagagccg cggcaaacgc gcgggtcaaa cgggtcatgg taaaactcct
    27721 tcgaatgttg ccgcgaaatg ccgtctgaag ggcttcagac ggcatttgcg ggattagaac
    27781 gtcgtgccga gttggaattg gaagcgttgg atttcgtctt ccggtttttt cttcagcggg
    27841 taggcgtagc tgaatttcat cgggcctaaa ggcgagagcc aggtaaccgc gccgccggcg
    27901 gaatagcgca attcgttggt aaaggtggat ttatgggtat tgccggcgcc gtaaatgttt
    27961 tgaaccctgc cgccggtcgc ggaactgctg ttgtcgtcgt aggttttgcc gtcccacacg
    28021 ctgcctgcgt cggcaaacag gctcaggcgg acggtgcgcg cgtctttcgc gccgggcatc
    28081 gggaagagca gctcggcgga gacgttggct tttttgttgc cgccgtagct gattttttcg
    28141 ccgtattcgt catagacttt cggaccaagt gtgccgcttt cgtaacctcg taccgaaccc
    28201 aggccgccgc cgtagaagtt ttcaaagaag gggatttctt tggttttgcc gtagccgttg
    28261 gcatagccga tttcgccgcc aagcatcagt gtgaaggttt tgctcagggg gaagaaccag
    28321 gtttggttgt gggtggcgga gtagtattgc agtttgctgc cgggcagggc gatttcggcg
    28381 ttcacgcccg tcaggtagcc gcgcgtcggc cataacgcgc tgtcggtttt gttgcgcccc
    28441 cagccgacgg tacctttgta cagccagcct ttgaagctgc cgtctgtgcc gtcggttttg
    28501 ccgtattgtt tgataaagtc ggcatagtgt ttgggcgctt tgttgtaggt gttgacggtc
    28561 aggtgttctg ccaccaaacc gaaattcacg cggtcgtatt cggtaacagg cacgctcatg
    28621 cggatgcctg cgcctgccgt ggtggtttta tattgtttga tgctggtcga tgctttgcgc
    28681 gggtcgaagg cttttccgta aacatcgtag cctaggctga ccccgtctgc cgtgaagtac
    28741 gggtcggtaa acgacagcga gccgttgagc gtggttttgc ttcgtgaggc gcgcagggcg
    28801 gccgacttgc ccgtaccgaa caggttgtct tgggaaacgc ctgcggacat gaccaggccg
    28861 gtatcctgta cccagcccgc gctcaagtcg agcgagccgg tggaacgttc ggtcaggctc
    28921 atgttcaaat cgactttgtc gggtgtgccg gcaagcggga cggcatcaaa ctgtacgttg
    28981 tcgaagtagc ccaaaagctc gacgcgctct ttggagcgtt gcagcttgga ggtgtcgtaa
    29041 ggcgcggatt ccatttggcg caattcgcgg cgcacgactt cgtcgcgggt tttgttgttg
    29101 ccggtgatgt ggatttcgtt gacgtagatt ttccggcccg gttcgatgtg caggacgaaa
    29161 tcgacggttt tggtttcggc gtttggcagc ggctgtacgc tgatttcgct gtatgcgtag
    29221 cctgccgagc ccatgcggtt ctgaatctca cccaaaacgg cggtcatctg ctggcgttcg
    29281 taccatttgc cgggcttcat ggtcagcagt ttttccagtt cggctttggg gacttcgttg
    29341 gtgtcgcctt cgatggagac tttgccccaa cggaaacgtc cgccttcgtg gacggtgatt
    29401 ttgatggtct gcttggtttt gtcttcgttg gtttggatgt cggtatcgag gatgcggaaa
    29461 tcgaagtagc cgttgttctg gtagaagtcg gttacttttt ccatgtcttg ggcaaatttc
    29521 tgctcgttga attggttgct tcgtgtcagc catgtccaaa tgccgccttc ggtcagcgac
    29581 atctgccgca tcagtttgcg gtcggaatag acttggttgc cttcaaattc gatgtcggtg
    29641 attttggcgg atttgccctc gtcaatcgtg atgtcgatgt cgacgcggtt gcgggcgagt
    29701 ttggttactt tgggcgtgat ttggatattg agtttgccgc gcccgaggta ttcttctttc
    29761 aggccggcga ctgcctgatt gagtgtcgcc tgattaaagt attgcgactg cgccagcccg
    29821 aacgattcga ggtttttctt aatggcgtcg ttttgcagca tttttgcgcc ggtgatgttg
    29881 agcgagccga tggtggggcg ttcgataacg gtcagcagga gctgcccgtc cgcagtttcg
    29941 acgcgtacgt cgtcaaagaa accggtggcg tacaggcttt tgatgatggc actgccgtgt
    30001 gtgtcgttgt aggtgtcgcc gactttgacg ggcaggtagt tgaatacggt actcggctcg
    30061 gtacgctgca agccttcgac gcggatgtct tggatggtga agtcggcaag tgccaaaggc
    30121 gatatgccca agaccatcag tgcggaagca atctgtttca gtttcattgt cagttccttg
    30181 tggtgcggaa tgcggtttca gacggcattc cgaaacgtaa aatctaaccg agcagccggg
    30241 taacgtcgtt gaagaaggcg accgccatca tcagcatcat gagggcaagc ccgaagcgca
    30301 aaccgatgtt ttggacgcgt tcgcccaaag gtttgccgcg tatccattcg gcagtataaa
    30361 acacgaggtg gccgccgtcc aaaacgggga cgggcagcag gttcagcacg ccgaggctga
    30421 tgctgaccag tgccaaaaat tccaaataac tttgcaagcc gagttcggcg gactgtccgg
    30481 caatgtcggc aatggtcagc ggcccggaaa tatggctgac ggaggcgttg ccgctgatta
    30541 gtttgccgaa aaatttgagg gttgtccacg agtgggaaac ggttttttcc cagcccatgc
    30601 cgaatgcgcg gataacagac ggacggtagc tgcggcggat ttgcgcgtcc cacgccctgt
    30661 ccggctgcgg acggaggccg acgcgcccga tcagggtgtg gtcgggctgt tcgacagtat
    30721 cggggcggat gtcggcggta tgggtttgtc cggcgcgttc gtaggtcagg gtgatttttt
    30781 tgccggggct ttggcgggtc aggtttgccc attcctgcca tgaggcgatg ggtttgccgt
    30841 cggcggcagt cagcctgtcg cccggtttca ggcctgcttt ttcggcgggg ctgccttttt
    30901 ccacgccgcc ggcaacggtt gtgattttaa agggcatcag tccgatgtag ccttggtttt
    30961 ttgcgatttt accggcttcc ggcgtgcctg cggcatcgat ggtgcggacg gtttgcgcgc
    31021 ccgatgccgt ctgaacgccg acggcgactt tgccggcttc gaggttgagg acgatttcgg
    31081 tttgcgcgct gccccaatct gcaacgggtg tgccgttgac ggattgtatt ttgtcgccgc
    31141 tttggaagcc ggcgcgggcg gcaatggtgt cgggttcgac tgtgccgaca taggggcgca
    31201 gttcggttac gccgaaggaa aagctcagtc cgtacagcaa aaccgccagt gcgagattgg
    31261 tcagcgggcc ggcggcgacg atggcgatgc gtttggcggg gtgttgtttg tcaaaagcgt
    31321 agggtaaatc ggcttctgat acttcgcctt cgcgcgtatc gaccattttg acgtaaccgc
    31381 ccaacggaat cggggcgagg caccattcgg tgtcgccgcg ctttcgggtg aaaaacggtt
    31441 tgccgaagcc gacggaaaag cgtacgactt tgacgccgca caatctggca acgatgtagt
    31501 gtccgaactc gtgcaggctg accaaaatca ggatggcgaa gataaaagct agaagggtgt
    31561 gcaaatggtt ttcctttgat aacggtgttc agacggcatc agcgcagtgt gccgataaat
    31621 gcccgcgctt gtgcgcgtgt ccgggcatct tgcgccaaga gcccccctat gtcgcctatg
    31681 ccgtctgaaa agtcttgtga aagacaatgg gcgacggttt tggcaatgtc ggtaaactta
    31741 atctgtccgt ccaaaaaggc ggcgacggcg gcttcgttgg cggcgttcaa tacgcagggc
    31801 gcggctccgc ctgcgttcat ggcttcatag gcgagcttca ggcaggggaa gcggtcaaag
    31861 tcgggctttt ggaaggtcag cgcggacaat gcgtcgaaat ccaggtcgcc gacacccgaa
    31921 tcgatgcgct cgggcaaacc caaacaataa gcaatgggcg tacgcatatc gggattgccc
    31981 agttgcgcca acacggagcc gtcgcggtag cgcaccatgc tgtgtatcac ggattgcgga
    32041 tggatgacga cttcgagctt gtcaggcgga cagttgaaca gccaatgcgc ttcaatcagc
    32101 tccaaacctt tgttcatcat ggtggcggaa tcgacggaga ttttgcgccc catgctccaa
    32161 ttggggtgtt tgaccgcttg ggcgggcgta atgctgtcga acgtgtttaa atcggcggtc
    32221 agaaacgggc cgccggaagc ggtcaggata atcgaagcga tgccgtgttc gttcagacgg
    32281 ccggcgtaat cgcgcggcaa aacttggaaa acggcgttgt gttcgctgtc gacgggcagc
    32341 actgccgcgc cgtttgcacg ggcggtttcc ataaacaacg cgccggaaac caccagcgtc
    32401 tctttgttcg ccagataaat ggttttgcct ttttgcgccg ctgcgagcgc ggaaggcagc
    32461 cccactgccc cgacgatggc gcacatgaca ccgctgactt cgtcggcaga cgcaacgtca
    32521 accaatgcct gcgcgccgtg taaaacctgc gtcgccgtgc cgtcgcgttt caacagggct
    32581 tcaagccggg cggcgtgttc cgcatcggca acgacggcat attcggggcg gaacgtttga
    32641 cattgagccg ccaatttctc gacctgctta tgccctgcca gcgcgaatac gcggaatttt
    32701 tcggggtggc gggagacaac gtccagcgtg ctttcgccta tgctgccggt actgcctaat
    32761 atggtcagga cttgtggtgt cataatgagg ataactttat accggatgcc gtctgaagcg
    32821 ttttcagacg gcatagaatc aatttaaaac cgacatcatc gccgcataga cgctgataac
    32881 ggcaatcagg ctgtcggtac ggtcgaacac gccgccgtgt ccggacagca ggttgctgct
    32941 gtctttaatg cccgccgcgc gcttgagcca gctttccaac aggtcgccgc atacgctgac
    33001 aacggtcagc accaaaccga ttaacacggt atcgaaccag cctgtatcga atgccagcca
    33061 gccggcactt cgtacggcgg tcatgtacac tgccacgcaa accgcgccgc cgattgcacc
    33121 ttcccagctt ttgccggggc tgattgccgg tgcgattttg tgtttgccga acgccttgcc
    33181 gctgaaatac gcgcaaatat cggcaaccca caccaaaccc atcacggcga gcagcggcag
    33241 ggcatcatcg ggatgcgggc gcagtgatac gagcgcgaac caaaacggca tgaccagaag
    33301 ccagccgacg gcataaacct gccaaccgcc gttgagcctc catttgaatc tcaaccataa
    33361 aggcataacg gcgagccaaa atgccaaaac aacataccaa accaaattag gcagcatcca
    33421 gccgcccgca taggcaacca cgccgaaaac caaggttgcg gcgaggtaat ggttggtttt
    33481 aattttgcac aaaccgccca tacgggcata ttcccacaag gcaatcaggg caatcagtcc
    33541 gcaaaatgca gcccacaacc attgcggcgc gtaaaacagc atgcccagca tcagcggcag
    33601 cagccacatg gcggttatta cccgttgttt cagcatattc agttcctttg ctgttcgata
    33661 ggcagttgct cggaggtgcg tccgaaccgc cgttcgcgtt tttggaacga agcgacggca
    33721 tcgtccaaag ccttgccgtc aaaatcgggc cacaaaatat cggtgaaata cagttctgca
    33781 tatgccatct gccagagcag gaaattgctg atgcgcgttt cgccgccggt gcggatgaac
    33841 aaatccggtt ccggtgcatc gcccagcatc aagtgtttcg ccagcgtgtc ttctgtaatc
    33901 tcggatacgc cttcggcaat cagtttgttt gccgcctgca aaatatccca gcggccgccg
    33961 taatcggcgg caatgctcag ggtcaggccg gtattgtttg ccgtcaacgc ctccgcttct
    34021 tcgatgcctt gcagaatctg ccggttgaag cgttcgcggc tgcccagtat tttcaggcgc
    34081 atattgtttt cgtgcagacg gcgtacctgt ttttgcaaag cctgtaaaaa cagccccatc
    34141 aggaacgaaa cttcgtcttc ggggcggcgc cagttttcgg ttgaaaaggc aaacacggtc
    34201 agatattgca cacccagttt ggcgcaatgc ttcaccatat tttccaatgc gtccaaaccg
    34261 cgtttgtgtc ccattatgcg cgggaggaaa cgttttttcg cccaacggcc gttgccgtcc
    34321 ataatcacgg cgatatgctt gggaatggcg gtgtgttcca aaacggcctg cgtgctgctt
    34381 ttcatgtctg cctttcgcgg ttcggcattc aaatgccgtc tgaacgccga accgtgcagg
    34441 ttaaattgcc atcaaatctt cttctttggc agtcaggagt ttgtcggctt cggtaatgta
    34501 tttgtcggtc agtttttgaa ccgcttcttc gccgcgacgt gcctcgtctt cggaaatttc
    34561 tttgtctttg aggagttttt tgatgtggtc gttggcatcg cggcgcacgt tgcggataga
    34621 gacgcggcct tcttccgctt cgccgcgtac gactttaatc aggtctttgc ggcgttcctc
    34681 ggtcagcatg ggcatcggca cgcggatcag gtcgccgaca gctgccgggt tcagccccaa
    34741 gtttgaatcg cggatggctt tctcgacttt ggccgccata ttgccctcaa acggtttcac
    34801 gccgatggtg cgcgcgtcca gaagcgttac gttggcaact tggctgacgg ggaccatgct
    34861 gccccagtat tcgacttcca cttggtcgag caggccggta tgcgcgcggc cggtacgcac
    34921 tttcgccaga ttttctttca gtacttcgac cgaacgctgc attttgcctt cggctgtttt
    34981 ttgaatatcg ttgatcatat tgttctttcg gtgggataag gtgggcggga gaccgtctga
    35041 acgcgtttca aacctttcag acggcatgaa aactgttaac tgcgaatagt accgttattc
    35101 gggcatgacg acaaggcagg cggattgggg atgccgtctg aagcggcagg cgtttcagac
    35161 ggcatcgtgt ccgaccgtca gccgtgttcc cgtgtttcaa gcaggctttg gcgcaggtgt
    35221 tggcgttcgt gggcatccag ccatttgcgg cgggtgcgtt gcagcaggat gacgagggcg
    35281 gaaatttcct gacgcatatt ggtgctgagc cagaggaagc cctgccattg gtagtggagg
    35341 tgttcggcga gggcttccag ttcggggttg atggcggtgt cgatgcggat gcggcgggcg
    35401 tgtctgccgt tgataagggc gacggtttgt tgcaggtcgg tttggagcag tgtgaagtgg
    35461 cggtcaagca gccggatttc gctgccgttg agtttgggag attgcagctt ggcggcagtg
    35521 gtcaggagca gctcggtggt gttgacaatt ttacggtggg cgtgctgcat ggcttccatc
    35581 atggcggggc tgatgcggct ttcgcccgat gtggcggcga ggtggctgcg gcttttgacc
    35641 atgcgtgcgt tgatttggcg cattttaacc atattctgct ccaaacgttc gcgcgtcata
    35701 cgcctgccgt tgctgatttc ggcaatcatt ttgctgcagt cggtcaggtt gtcggcaagc
    35761 atgaaacgcc acatcagtgt ggatttcagc ggcagcagtt tggcggcggc gatggcgatg
    35821 gccgcgccga tgaggacgtt catcgcgcgc atcaggccgc tgtcgaacca ttcgctgccg
    35881 ttgtcgccga tgagcatgca catcgtcagc cccgccagca tagggacgta gccgtttttg
    35941 ccgaccgccg cccagccggc cagtgcgctt gccgtgccga cggtgaggta gaagaggagg
    36001 ttgccgtgga aataatgctg gttcagccat aaaacgccca aacccgcgcc cagcccgatg
    36061 accgtgccga gcatacgttc caccgccttg gagtaaatcg ccccttgaaa ctggagcatg
    36121 ccgaggacga cgaagacggt catgcctatc cattcgccgt gttggaggtg gagcagccgg
    36181 gcgagtgcgg tggcgaacag gacggccccg ccgagccgca cggcgtgtat gaggcggcgg
    36241 tggcggtagc gttcgtagga gttgagccag cggctgacga ggcggttgcg ttgcgaggtg
    36301 ttcatatcgg ttgccgtctg aagcggaaat gtgaaaaagc acaggcttcc cgaggaaggg
    36361 agggtctgtg cttggtattg gtgccggaga agggaatcga acccccgacc ttcgcgttac
    36421 gaatgcgctg ctctaccgac tgagctacac cggcgttttt tcgttatgat atatatgaac
    36481 ggttgtttgt gcaacttttc tggcgggcgg caaggcagtg cgcggtataa tgcggtctgc
    36541 ttcggaagag ggggacggcg atgtttgtga acgagaaaga tccttatgcg gctctgtttg
    36601 cggggctggt gtttttgacg ttgccgtttg cgttggcggt gcatgatgcc tttgcgcttg
    36661 cgttcggacg ggcagggctg ctggtgtcgg tgtcggccgg cgtggcggtt gggacggtac
    36721 tgtttggttt gtgttcggtg tgtttgcgtt tttgaacctg gtcgtgtcgg caggcctgac
    36781 gaagctggcg tacaaaaaga tgatgcggcg gcattcccgt tacgcgctgt ttctgtcggg
    36841 cgtggcggct tgcgcggcgg cagcggtggc ttggattttc gagctgctgc ttggcagtgg
    36901 ggctttgggc gggctgcgtg gggaggcggt gttggaatat gcgtttgccg tgtggctggt
    36961 ggcgatgctg acgctgccca aacgcctgac gcgcgcgccg gtgcagccgg tggtgtttca
    37021 caggaaaaaa taggttggaa cgggaaatgc cgtctgaacc ccgacacgcg gtttcagacg
    37081 gcatgttttt ccgttaacat tacgcctgaa tatggacagg aagcagatat ggaacgcaaa
    37141 gaacgcctgc aatcaggcat tgccgcgatg gggctggata tttcggaaac ggcgcaggac
    37201 aggcttttgg cctatgtgga tttgttgaaa aagtggaaca aaacctacaa tctgaccgcc
    37261 ctgcgcgacg aggaaaaaat gattgtccat catcttttgg acagcctgac gctgctgccc
    37321 catatcgagg gtgtgcaaac gatgctggat gtcggttcgg gcggcggtca gcccggcatt
    37381 ccggcggcgg tgtgccgtcc ggatgtgcag ataacccttt tggatgcgaa tacgaagaaa
    37441 acggcttttt tacagcaggc ggttatcgag ttggggttgg acaatgtgcg cgtggtatcc
    37501 ggacgcgtgg aggcggtttc ggacgtgcgt gccgacgtgg ttaccagccg tgcgtttgca
    37561 gaactggcgg attttgtgtc gtggacggtg catctgttga aagacggcgg ctactgggcg
    37621 gcgatgaagg gcgtgtatcc gcagggggaa atcgaccacc tgccgcagga tgtgtgcgtc
    37681 gaaaaagtcc aaaggcttga cgtgccgggc ttggatgcgg aacgccatat cgtcatactc
    37741 cgcaagcgtt gagcgcgctt cagacggcat gaataccttt tttgtgcgga taaaggtaaa
    37801 attccgcact gtttttcttt tttcaacatc agacgggaca cgggcgggac atgagtgcga
    37861 acatccttgc catcgccaat cagaagggcg gtgtgggcaa aacgacgacg acggtaaatt
    37921 tggcggcttc gctggcatcg cgcggcaaac gcgtgctggt ggtcgatttg gatccgcagg
    37981 gcaatgcgac gacgggcagc ggcatcgaca aggcgagttt gcagtccggc gtttatcagg
    38041 tcttattggg cgatgcggac gtgcagtcgg cggcggtacg cagcaaagag ggcggatacg
    38101 ctgtgttggg tgcgaaccgc gcgctggccg gcgcggaaat cgagttggta caggaaatcg
    38161 cccgggaagt gcgtttgaaa aacgcgctca aggcagtggc ggaagattac gactttatcc
    38221 tgatcgactg cccgccttcg ctgacgctgt tgacgcttaa cgggctggtg gcagccggcg
    38281 gcgtgattgt gccgatgttg tgcgaatatt acgcgctgga agggatttcc gatttgattg
    38341 cgaccgtgcg caaaattcgt caggcggtca atcccgattt ggacatcacg ggcatcgtgc
    38401 gcacgatgta cgacagccgc agcaggctgg ttgccgaagt cagcgaacag ttgcgcagcc
    38461 atttcgggga tttgctgttt gaaaccgtca tcccgcgcaa tatccgcctt gcggaagcgc
    38521 cgagccacgg tatgccggtg atggcttatg atgcgcaggc aaagggtacc aaggcgtatc
    38581 ttgccttggc ggacgagctg gcggcgaggg tgtcggggaa ataggtcaat ccaaatcggg
    38641 ctgcccgtgc ctttatgctg tttggccggg tgcgttaata tggcggatta aaataaaaat
    38701 acttatatcg t
//
LOCUS       DAXHTR010000003        35166 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.003, whole genome shotgun sequence.
ACCESSION   DAXHTR010000003 DAXHTR010000000
VERSION     DAXHTR010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35166
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.003"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(144..234)
                     /locus_tag="ACFNML_000099"
     tRNA            complement(144..234)
                     /locus_tag="ACFNML_000099"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(198..200),aa:Ser,seq:gga)
     gene            complement(273..359)
                     /locus_tag="ACFNML_000100"
     tRNA            complement(273..359)
                     /locus_tag="ACFNML_000100"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(323..325),aa:Leu,seq:cag)
     gene            complement(395..481)
                     /locus_tag="ACFNML_000101"
     tRNA            complement(395..481)
                     /locus_tag="ACFNML_000101"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Leu,seq:cag)
     gene            570..2945
                     /gene="rnr"
                     /locus_tag="ACFNML_000102"
     CDS             570..2945
                     /gene="rnr"
                     /locus_tag="ACFNML_000102"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFC6391564.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGIDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRQ
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKTQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLATLREQL
                     GLLGLQLGGGDNPSPKDYAALVEQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEVFEGKISGMTSFGIFVTLDGIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAVRVARADLDDGKIDFVLIAGGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAEKKTARGGKVRGRGASAAAESGKKAKKPVPIKVKKRKGKS"
     gene            complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="ACFNML_000103"
     CDS             complement(3284..4747)
                     /gene="guaB"
                     /locus_tag="ACFNML_000103"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFC6391565.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(4888..7446)
                     /gene="glnD"
                     /locus_tag="ACFNML_000104"
     CDS             complement(4888..7446)
                     /gene="glnD"
                     /locus_tag="ACFNML_000104"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HFC6391566.1"
                     /translation="MPANLSSALETFKQQRDAAEAHYLKANRVSVFFREYTAALETLL
                     AALWAEYFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVDELCESVRNDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYEGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLQSRVSSTPLRVTLRIDDDYIQVNNQIAARHT
                     DIFFRRPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNSENRHRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIVGLLQHDLFHIYPVDDHILAVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGISDARQFAADHF
                     LTEEESDLLAWLVENHLLMSIVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLAGNGGNPHALFGRRQQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPKSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETSNHSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEIFFAHNVSLRYAKISTLDERAEDSFTVFSPDLKNPKIQSSLKQTLLEQLS"
     gene            complement(7495..7815)
                     /locus_tag="ACFNML_000105"
     CDS             complement(7495..7815)
                     /locus_tag="ACFNML_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6391567.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLKLMTNSADTRQMPS
                     ESGI"
     gene            complement(8139..8612)
                     /gene="bfr"
                     /locus_tag="ACFNML_000106"
     CDS             complement(8139..8612)
                     /gene="bfr"
                     /locus_tag="ACFNML_000106"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6391568.1"
                     /translation="MKGDRLVIRELNKNLGLLLVTINQYFLHARILKNWGFEELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(8640..9104)
                     /gene="bfr"
                     /locus_tag="ACFNML_000107"
     CDS             complement(8640..9104)
                     /gene="bfr"
                     /locus_tag="ACFNML_000107"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFC6391569.1"
                     /translation="MQGNQAVVDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMARAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVSRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            complement(9568..10551)
                     /gene="lipA"
                     /locus_tag="ACFNML_000108"
     CDS             complement(9568..10551)
                     /gene="lipA"
                     /locus_tag="ACFNML_000108"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFC6391570.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRESHGGCGHH"
     gene            complement(10544..11167)
                     /gene="lipB"
                     /locus_tag="ACFNML_000109"
     CDS             complement(10544..11167)
                     /gene="lipB"
                     /locus_tag="ACFNML_000109"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFC6391571.1"
                     /translation="MKVIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            complement(11169..11444)
                     /locus_tag="ACFNML_000110"
     CDS             complement(11169..11444)
                     /locus_tag="ACFNML_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HFC6391572.1"
                     /translation="MTEQKNKTSLIEFPCTFSLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQIKVENQEQLDNIYRALTSHELVKVVL"
     gene            11629..12699
                     /locus_tag="ACFNML_000111"
     CDS             11629..12699
                     /locus_tag="ACFNML_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFC6391573.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLAFALGDTLAPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYVEIDQASIIAWLQAHTGELSNTLKAWFPVLMRQGSNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            complement(12806..13753)
                     /locus_tag="ACFNML_000112"
     CDS             complement(12806..13753)
                     /locus_tag="ACFNML_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HFC6391574.1"
                     /translation="MEFFIILLAAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVSALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(13768..14175)
                     /locus_tag="ACFNML_000113"
     CDS             complement(13768..14175)
                     /locus_tag="ACFNML_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFC6391575.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIIYGLTGSTP
                     AAVLAAALLSALGIWFVHAKTAGGKVETDSYQDLDAGQYAEILRHTGGNRYEVFYRGT
                     HWQAQNTGQDVFEPGTRALIVRKEGNLLIITHP"
     gene            14256..14915
                     /gene="ung"
                     /locus_tag="ACFNML_000114"
     CDS             14256..14915
                     /gene="ung"
                     /locus_tag="ACFNML_000114"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFC6391576.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPETDVFNA
                     FRLTAFDRVKVVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVREGQAHSHALLGWERFTDTVIRQLATHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            complement(15022..15378)
                     /locus_tag="ACFNML_000115"
     CDS             complement(15022..15378)
                     /locus_tag="ACFNML_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391577.1"
                     /translation="MMKKQRHILCVGCLYIILKVNVFPNTGWMEASKGMQGISFSTFG
                     YADAPGYTFSRIVHTLIFLLGKTTGTRTTVSDSSHNVYYVKLLPKPSTKRLCRICRQA
                     NKSCFWKWRQKMPSEP"
     gene            complement(15423..15938)
                     /locus_tag="ACFNML_000116"
     CDS             complement(15423..15938)
                     /locus_tag="ACFNML_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391578.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(16404..18326)
                     /locus_tag="ACFNML_000117"
     CDS             complement(16404..18326)
                     /locus_tag="ACFNML_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6391579.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGEITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKFTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIIRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFVGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIAVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            complement(18403..18774)
                     /locus_tag="ACFNML_000118"
     CDS             complement(18403..18774)
                     /locus_tag="ACFNML_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HFC6391580.1"
                     /translation="MLFEENPIDGQFAEYECGAGGIRLAGQSFHKPVLVHKDSVCLSQ
                     CRTLSDLTPENLLSDIKPVDYPEILIIGTGAAQEFIHPKIMADFSRIGISVECMNTDS
                     AFRTLVFLHSEGRRAWAWLQP"
     gene            18971..20278
                     /locus_tag="ACFNML_000119"
     CDS             18971..20278
                     /locus_tag="ACFNML_000119"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HFC6391581.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAEEISRRLGREIRISAVCD
                     LSEEKARQICPSAAFVKDPFELVARKDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFPLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGHDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            20271..20705
                     /locus_tag="ACFNML_000120"
     CDS             20271..20705
                     /locus_tag="ACFNML_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391582.1"
                     /translation="MTEPKHEMPTEEQVAARKKAKAKIRTIRIWAWVILALLASTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLTVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(20764..21033)
                     /locus_tag="ACFNML_000121"
     CDS             complement(20764..21033)
                     /locus_tag="ACFNML_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HFC6391583.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(21215..23677)
                     /gene="lon"
                     /locus_tag="ACFNML_000122"
     CDS             complement(21215..23677)
                     /gene="lon"
                     /locus_tag="ACFNML_000122"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFC6391584.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAALEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIEAVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDADHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DKKRLSETKKTSKAKPKAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVG
                     GELLTVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIH
                     VHVPEGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKL
                     LAALRGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEP
                     SGAEAAAESASKPKPRSRATKH"
     gene            23928..25031
                     /locus_tag="ACFNML_000123"
     CDS             23928..25031
                     /locus_tag="ACFNML_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6391585.1"
                     /translation="MSSFIFPDNGAEWRNESVQKPPKGWHYVRESGADGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRARKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFADLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLHLRPSDFLDKAFAQAGLRVADVL
                     KTKPVHKKAADPNNPLAFARNRETTFLYRLKKA"
     gene            complement(25103..26848)
                     /gene="recD"
                     /locus_tag="ACFNML_000124"
     CDS             complement(25103..26848)
                     /gene="recD"
                     /locus_tag="ACFNML_000124"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFC6391586.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     DTSAPDAAGARQNLAKWFQGTGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPNIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFNPVYPLPFDVLVIDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHLAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPEFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSKELLYTAITRAREKFVFFGGEEAFRQAAATVKTR
                     QTALGSMLERVFSQE"
     gene            complement(26916..27611)
                     /gene="lolD"
                     /locus_tag="ACFNML_000125"
     CDS             complement(26916..27611)
                     /gene="lolD"
                     /locus_tag="ACFNML_000125"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFC6391587.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLSQRQLGILRNHHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFERVMVM
                     KDGSLHPKQGANA"
     gene            complement(27604..28851)
                     /locus_tag="ACFNML_000126"
     CDS             complement(27604..28851)
                     /locus_tag="ACFNML_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HFC6391588.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDTRVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDTVWVRDWTFSNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPVGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSWRASKTQPAEALRYE"
     gene            29056..29334
                     /locus_tag="ACFNML_000127"
     CDS             29056..29334
                     /locus_tag="ACFNML_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391589.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            complement(29390..30007)
                     /gene="recR"
                     /locus_tag="ACFNML_000128"
     CDS             complement(29390..30007)
                     /gene="recR"
                     /locus_tag="ACFNML_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFC6391590.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKSAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(30074..31612)
                     /locus_tag="ACFNML_000129"
     CDS             complement(30074..31612)
                     /locus_tag="ACFNML_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HFC6391591.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGAKLMGISVSSEQIKQIIV
                     DDPNFHDANGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQTVSETEVKNAFEERVARLPTNEAKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     DVLTQLNGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLIALP
                     APVIVEVQAVTPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            complement(31672..32046)
                     /locus_tag="ACFNML_000130"
     CDS             complement(31672..32046)
                     /locus_tag="ACFNML_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HFC6391592.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAGYGLEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKALFSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            32196..33824
                     /locus_tag="ACFNML_000131"
     CDS             32196..33824
                     /locus_tag="ACFNML_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFC6391593.1"
                     /translation="MISTNGITMQFGAKPLFENVSVKFGEGNRYGLIGANGSGKSTFM
                     KILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEDMRVLDVVMMGHTEMWAAMTERDAI
                     YANPEATEDDYMKAAELEAKFAEYDGYTAEARAAELLSGVGISEDLHNAKMAEVAPGF
                     KLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVLNQYDSTMIIISHDRHFLNEV
                     CTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNAKAKEKLQELQEFVARFSANK
                     SKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEADEKAKLHRQAVEVEKLAKRFE
                     TQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAYNPEYSDGLLPDEGSIKWAEK
                     ASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIRGTLGRLLFGSNDVVKKVKVL
                     SGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESLNMALEKYNGTLIFVSHDRQF
                     VSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            33896..35149
                     /locus_tag="ACFNML_000132"
     CDS             33896..35149
                     /locus_tag="ACFNML_000132"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HFC6391594.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWFALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKTFRAAQDKTEKH"
BASE COUNT         7718 a         9065 c         9650 g         8733 t
ORIGIN      
        1 taccgcaaaa cataccataa aaaaaacggt aacggatagt tgtaagcggt tgatgacgat
       61 tatagacaat aacgggtttt cccaatgaaa ttattgtttg aataataaaa aatcccaaac
      121 cgtaaaagtt tgggatttgt atttggcaga gaggaaggga ttcgaaccct cgatacgcta
      181 ttcacgtata cacgctttcc aggcgtgcga cttaaaccac tcatccacct ctctaatggc
      241 ggaaattatc ccaatcggga taatttatta tttggtgccc gggagaagac tcgaacttcc
      301 acacccatga ggataccagc acctgaagct ggcgcgtcta ccaattccgc cacccgggca
      361 atctaaatat tgaaataaag caaagcattt gatttggtgc ccgggagaag actcgaactt
      421 ccacacccgt gaggatacta gcacctgaag ctagcgcgtc taccaattcc gccacccggg
      481 ctttgcttta tttgctgttt tgcggtacag tgtcctgccg caaagaagtc gctattatat
      541 agttcataaa gagaatgtca acagtccaaa tgaataaaaa tattaaatct ttaaatttac
      601 gggaaaaaga cccgttttta agtcgtgaaa aacagcgtta tgaacatcct ttgcccagtc
      661 gggaatggat aatcgaattg ttggagcgca aaggtgtgcc ttcaaaaatc gaatcgcttg
      721 cgcgcgagct gtcgattacg gaagacgagt atgtcttttt cgaacgccgt ctgaaggcga
      781 tggcgcggga cggtcaggtt ttaatcaacc gtcggggcgc ggtttgcgcg gcggacaaat
      841 tggatttggt caaatgccgc gtcgaggcgc ataaggacgg tttcggtttt gccgtgccgc
      901 tcacgcccgc caaagacggt gattttgttt tgtatgaacg ccagatgcgc ggcattatgc
      961 acggcgatat tgtcactgtt cgtcctgccg gcatcgaccg taggggccgc cgcgaaggga
     1021 cagttctgga tattgtcgaa cgcgcgcaaa gcaaagtggt cggccgtttc tatatggata
     1081 ggggcgtggc gattttggag ccggaagaca agcgtctgaa ccaaagcatc gtattggaac
     1141 cggacggcgt ggcgcgtttc aaacctgaat ccggtcaggt catcgtcggc gaaattgagg
     1201 tttatcctga gcaaaaccgg caggcagtgg caaaaatcat cgaagttttg ggcgattatg
     1261 ccgacagcgg catggagatt gaaattgccg tgcgcaagca tcatttgccg caccaattca
     1321 gtgaagcgtg tgccaaagcc gtgaaaaaaa ttcccgacca tgtacgcaaa agcgatttga
     1381 aaggccgcgt cgatttgcgc gacctgcctt tggtaacgat tgacggcgaa acggcgcgcg
     1441 attttgacga tgcggtgttt gccgaaaaaa tcggacgcaa ttaccgtctg gtcgtggcga
     1501 ttgccgatgt cagccattat gtccgccccg atgacgctat cgacacggac gctcaggaac
     1561 gcagcaccag tgtttacttc ccgcgccgcg tgattcccat gttgccggaa aacctgtcca
     1621 acggcatctg ctcgctcaat cctaatgtcg agcgtttgtg tatggtgtgc gatatggtca
     1681 tcacttacgc gggcaatatt aaagaatacc gcttctaccc cgccgtgatg cgctctcatg
     1741 cccgcctgac ctacaaccaa gtttggaaat ggctttcagg cggcatcggg catccgttca
     1801 aaacacaaat cgacacgctt tacaaactct tcaaaatcct tcagaaaaag cgtttcgaac
     1861 gcggggcggt ggagtttgac agcatcgaaa cccaaatgct tttcgacgac aacggtaaaa
     1921 ttgaaaaaat cgtccccgtt gtccgcaacg atgcccacaa gctgattgaa gaatgtatgc
     1981 tggcggcgaa tgtttgcgcg gcggattttc tgttgaaaaa caagcatacg gctttgttcc
     2041 gcaaccattt gggccccacg cccgaaaaac tcgccaccct gcgcgagcag ctcggtctgt
     2101 tggggcttca acttggcggc ggcgacaacc cgtcgccgaa agactatgcc gcgcttgtcg
     2161 aacaattcaa aggcagacct gatgccgaat tgctgcaagt catgatgttg cgctccatgc
     2221 aacaggcggt ttacgaaccg cattgcgacg gacactttgg tcttgcctac gaagcatacg
     2281 cccacttcac ctcgcccatc cgccgctatc ccgacctgac cgtacaccgc gccatcaaag
     2341 ccgtgttgaa tcagcaaacc tacacgccaa aaaaaagctg gcaggctttg ggcgtgcata
     2401 cctcgttctg tgagcgccgt gccgacgacg ccagccgcga cgtggaaaac tggctgaaaa
     2461 cctattatat gcgcgataag gtcggcgaag tattcgaagg taaaatctcc ggcatgacca
     2521 gttttggtat ctttgtaaca ctggacggca tccacattga cggcttggtg catatcagcg
     2581 atttgggcga agactatttc aacttccgcc ccgaaatcat ggcaatcgaa ggcgaacgca
     2641 gcggcatccg tttcaacatg ggggacaggg ttgccgtccg ggtcgcccgt gccgatttgg
     2701 atgacggaaa aatcgatttt gtcctgattg ccggggggag cggcaggggg cggaaagtta
     2761 aatcatccgc gtctgccaaa ccggcaggga cggcggggaa agggaagccg aaaaccgccg
     2821 ccgagaaaaa aacagcccga ggcggcaaag taaggggaag gggcgcgtct gccgccgcag
     2881 aatcggggaa aaaggcaaag aaaccggttc cgattaaggt aaaaaaacgg aaaggcaaat
     2941 cataatgctg acggggtggc ttggggaggg cggggcatac tttcccgaag ccgccgcccg
     3001 attttttgat atggcggccg gcgcatcatt tcaggccaat cgaaacgccc gtattgaaag
     3061 attgcgttta tttccaccgc cgttttaaag gccggcggta ttcggcagac ggggcgcaaa
     3121 cggcgttcag acggcatttt cattctttcg gcgtgtccgt ccgaattgct ttgcccgtcc
     3181 gcgcaatcag cccgtgcggc ttgccttgaa cggacaaaaa atgccgtctg aaacccgaaa
     3241 atcaggtttc agacggcatt ttgccttgaa aaggctgttc aaatcagcgg tggtagttcg
     3301 gtgcttcttt agtgatttga acgtcgtgaa cgtgcgattc gctcatacct gcggaagtga
     3361 tttccacaaa ttcggctttt tcgtgcattt cggcaatatt ggcgcaaccc aagtaaccca
     3421 tactggaacg cagaccgccg gtcagctggt ggatgatgtt cacaatcggg cctttgtaag
     3481 gaacgcggcc ttcgatgcct tcggggacgt atttgtcggt gctgtcggtt ttgtcttgga
     3541 agtagcggtc ggcagaacct tggctcatcg cgcccaatga acccataccg cgataggatt
     3601 tgtacgagcg gccttgatag agttcgattt cgcccggcgc ttcttccgtg ccggcaaaca
     3661 taccgccgag catgacgctg tacgcgcctg cggcgagggc tttggcgatg tcgccggaga
     3721 agcggatgcc gccgtcggca atcaggggaa cgcctgtgcc tttgagggct tcggcaacgt
     3781 tgtggatggc ggtcagttgc ggcacgccca cgcctgccac gatacgggtg gtgcaaatcg
     3841 atcccgggcc gataccgact ttgacggcat ccgcaccggc ggcgaccaaa tccaaagcgg
     3901 ctttggcagt ggcgatattg ccgccgatga cttggatgtg cggataggtt tctttcaccc
     3961 atttcacgcg gtcaatcacg ccttggctgt gcccgtgggc agtatcgacg acaatcacgt
     4021 ccacgccggc ttcgaccaag gctttgacgc gctcttcggt atcgccgccg gtgccgactg
     4081 ccgcaccgac gcgcagacgg ccttcggagt ctttgttggc gttgggaaac tcggtggttt
     4141 ttaaaatatc tttaacggta atcagacctt tgagttcgtc tttttcgttc agaaccaaaa
     4201 cgcgctcgac tttgtgcgtg tgcatcagtt cgcgcgcttc gtctatgctt gtgccttcgg
     4261 ggacggtaac cagacgttcg cgcggggtca taatggcgga aacgggcaaa tcgacgcggt
     4321 tttcaaaacg caggtcgcgg ttggttacga tgccgacgac tttgccgttt tcaacgacgg
     4381 gcaggccgga cattttgcgt ttgcgctgcg cacgcatttc caagacttcg cggatgagcg
     4441 tcgtcggtgc aacggttacg gggtctttga ccacgccgct ttcgtggcgt ttcactttgg
     4501 aaacggcgcg cgcctgcatt tcgggcggca tgtttttatg gatgatgccg atgccgcctt
     4561 cctgtgccat cgaaatggcg aggcgcgcct cggtaacagt gtccatcgca gcggaaagca
     4621 gggggaggtt gagtgtgatt tcgcgggtga gcttggtttg aagtttaacg tctcgcggca
     4681 gcacggtcga atgtgcggga accaacaaaa catcgtcgaa agtataggct ttttctacga
     4741 tacgcataat gctcggtctt tcagtttgtg caagatgcac ggcattatag cacgttaccg
     4801 gcggcttgac agtttatatt gtttgcaata tcccccgcct tcaggcggca tacggcgtgg
     4861 cggcggaatg ccgtctgaag gcgggcgtta tgataattgt tccagcagcg tctgcttcaa
     4921 tgaggactgg atttttgggt ttttcaggtc ggggctaaaa acggtaaaac tgtcttcggc
     4981 gcgttcatcc agtgtggaga ttttggcata gcgcaggctg acgttgtggg cgaaaaagat
     5041 ttccgccata tcggcgagca ggaaggggcg gttgacggcg gtaatttcga cggagtacca
     5101 gtcggggtag tcttcttcgg gggtaatggt gatgcttggc gcgatcggca tgtagcggct
     5161 gcggcggctg atgcggcggc tgtggtttga ggtttcggca acggtgtgtc cgtggataaa
     5221 gctgttgagt tcggcttcaa gcgcgctttg aatgtcgggg tagtcttcgg gggcgtgttg
     5281 tgagggaatt tgcacgatga aggtgtcgag gatgtagtcg tgttcggtga taaaggcgcg
     5341 ggcggcgagg atatcgaagc cgtggcggct gaaaatgcgg cagagacggg caaacaggcg
     5401 cgggccgttg ggcatgaaaa ccatgacttg aaagctgtcg cttttgggca ggatgcggct
     5461 gcggacgatg ggggtttcaa agtcgtggac taggttggcg gcgtgccaca ggatttcgcg
     5521 ggactggtgg cgggcgaagt aggcggaacc gagcgcgttc caaagctttt tctgctgttt
     5581 ttcggggacg gcggcgcggg tgagcaagtc ggcggcttct tgttggcggc ggccgaagag
     5641 ggcgtgcggg ttgccaccgt tgcctgcgag gtagcgtccg gcggcatgga agaggctttc
     5701 cagcaggctg gcgcgccatg cgttccacag cttgggattg gtaccgcgta tgtcggaaat
     5761 ggtcagaagg tagagcgcgc tgaggcgttc gtgggtttgg acgcgtttgc agaaggcatc
     5821 gagtacgtcg gggtcttgga tgtcttcttt ttgggcgacg atggacataa gaagatggtt
     5881 ttcgaccagc caggcaagca ggtcgctttc ttcttcggtc aggaagtggt cggcggcaaa
     5941 ttggcgcgcg tctgagatgc cttgtatggc atggtcgccg ccgcgtcctt tggcgatgtc
     6001 atggaaaaag gcggcaaggt agaggatgtc ttgtttttca aaggactgca tcagtgcaga
     6061 ggcgtagggc agctcgtggc tgtgcatatc caaggcaagg cggcggacgt tgcggacgac
     6121 ggcgaggatg tggtcgtcca cgggatagat gtggaacagg tcgtgctgga gcaggccgac
     6181 gattttttcc cacgcgggca ggtagcgtcc caatacgccg tagaggttga ggaagcgcag
     6241 ggtttgggtc agtccgttgc cgttgcggaa aaagccggcg aagcggtggc ggttttcaga
     6301 attttggtag aagctgcggt tgattttgcg cgtcgcgccc caccatgcgc gcagggtttg
     6361 cggttcgagc gcggtaatgt cgttgcgctg ctgcatgatt tcgacgattt tgaaaatgtg
     6421 ttcgggccgt ctgaaaaaaa tatcggtgtg ccgcgcggcg atttggttgt tgacttggat
     6481 gtagtcgtcg tcaatccgca gggtaacgcg caacggggtg gaggaaacgc ggctttgcag
     6541 cataggcgtg aggatgccgc ccagttgttt gacggtttta atcgcgcggt aaaacacgcg
     6601 catcagttct tcgctttggc ggcggaggtt caagccttca taacccatgc tttcggcgac
     6661 ttgcggctgc aaatcgaaca gcaggcggtc ttcggcgcgc ttggcgttta aatgcagatg
     6721 gatgcggatg tgggcgaggc ggcggtagcc gtgcgaaagc ataccggctt cggcacgcgt
     6781 caaaatccgc tgtttgagca ggtcgggcag gtcggtcgcc aagccttgcg ccttcgctat
     6841 ccaaagcagg gtgtggatat cgcgcagacc gcccggacag cttttgatat tcggctccaa
     6901 tactgccccc gaaccttgcg atttggcgtg gcggtgttcc atctccacca gttttgcctc
     6961 gacaaacgcc gccacattgc gctgcgcgtt cattttttcc gctagtttat ccgccgtttg
     7021 gcggttgcca aacaaaaacc tagcctctaa aaacgctgtg tcccccgtaa tatcattgcg
     7081 cacgctttca catagttcat caacgctgcc gctttttaca gacggcatca gtttgcagtc
     7141 ccacagggtt tggacaaacc gggcaatctg ttcctgaatg ccgtctgaaa gcggggcagg
     7201 ggagacaacc gccaaatcca catccgaaca gggatacagt tcgccgcgtc cgaagccgcc
     7261 taccgccatc aggcataacg cgctgttttg aaaatactct gcccacaatg ccgccagtaa
     7321 ggtttcgagt gccgccgtat attccctgaa aaataccgac acgcggttgg ctttcaaata
     7381 atgcgcttcg gcggcatcgc gctgctgttt gaaggtttcc agtgctgaag acaggtttgc
     7441 aggcattttt attctttcga ttggcgggaa aaagggaggc ggatggttcg gcggtcaaat
     7501 accgctttca gacggcattt gtcgggtatc ggcggaattg gtcatcagct tcaaccgttc
     7561 ctgcggcggc agcagcaact gatacgccgc cacgcccaaa gccgccgcca ttttttcgat
     7621 attcgacagg gcgatgttcc agcgtttgcg ctcgactgcc gacacataag tcctgtccaa
     7681 accgcattgc cgcgccaatt cttcttgcga ccaacccttg ttcacgcgga aaagccgcat
     7741 attgtatgcc aataccgccc gcaaatcctg ttcgtcaggc agttcggcag gcagagtcaa
     7801 tttgttgccc atcatttgct tccagataaa tggttaaagt taagcatttg ctgttacgga
     7861 ttttacttca cataaaagcc aaaaaaaagg ggtggcggca gccacaccca aacacacaca
     7921 catcaagagg aaagagataa aatcagacag attggaaaac agcaaacaag caccctgaat
     7981 ttcatatcgg tattatcaaa caaaaaccct tatcaggtat tgattcaaat caatttagat
     8041 tttattcgca aaccgaaaaa gaaaaacggc atatccgttt atacggatat gccgtctgat
     8101 ggtgcggggg actgctgtat ttggcagtag tttgtgtttt agtcctcttg cgccgctgtt
     8161 tgcaggtagt tgggcagacc gattttgccg ataagctctt gctgggtctc cagccagtct
     8221 atatgttctt cgttggtgtc tttttgtttt tccaacaaat cgcggctgac gtaatcctgt
     8281 tgcgcttctg ctgtggcgat ggcggcaagc agggcttcgt gtttttcctg ttctttggtc
     8341 aaatcgcagg cgatgatttc ttcggtggac tcgccaatca gaagcttgcc cagttcttgc
     8401 aggttcggca gaccttcgag gaacagaatg cgctcgatca aatcgtcggc agctttcatt
     8461 tcaacgatgg actgtttgaa gaaatgttcg cccagttctt caaagcccca gtttttcaaa
     8521 atacgggcgt gaaggaaata ttggttgatg gttaccagca gcaagcctaa gtttttgttc
     8581 agctcgcgga taaccaaacg gtcgcctttc atacggactc cttttattcc gaatacggat
     8641 tacagttggc tttggtagta gttgccttcg ccaatcagct cgatcaggcg cagctgctgt
     8701 tccagccagt gggcgtggtc ttcttcggta tctttcagtt gggcgaccat caaatcgcgg
     8761 ctgacatagt cttgcgcctc ttcgcacagt ttgatgcctt ttttcaaagc gtcgcgcact
     8821 tcgtattcgg tttgcaggtc ggctttgagg caggaaacca cgtccgtgcc gatattcagc
     8881 tcggcgcgcg ccattttcgg cgtaccgccc agcatcagga tgcgtcggat gaagtcttcg
     8941 gcgtgtgtgg tttcttcttc catctcgtga ttgagacgtt caaaaagttt ggtgtagccc
     9001 cattcggagt agaggcggga gtggataaag tattggtcgc gtgccgccag ctcgccagac
     9061 agcaattcgt tcatataatc aacaacagct tgattgcctt gcataatatc tctctttccg
     9121 taacttggtt ttcggtatgt ctgaacagca tctgtgtcgc tattcgggat gcgtgcattg
     9181 tatgcaaaag ctgtcggcat gacaaatttt ctatttaaaa tacaaacaat tatcaaaaga
     9241 aatagggcgt attccccgat gccttccaaa tcagtatgct gtttcttatc ggttttttgt
     9301 gctgttaaaa ctaagaatcc atatcatcca taaagaacat ttacacttgt taaccatagc
     9361 gaaaagcaaa tactgtacgg ttttttatca aaatttataa tgaatcgttc tcattaaccg
     9421 acagattctt ttgagattat cggattttgg gaataaatta tgccgtctga ggggctttca
     9481 gacggcatag acaattaaag ctgattaaac aacgcggcag acagcagcgt aggaaattat
     9541 tttttacttc ggaacggtat tttgggttta atgatgcccg caaccgccgt ggctttcgcg
     9601 taaggcttcg gcagcctgtt cgtcggcgtg gtagctggaa cgtaccatcg cgccgatggc
     9661 ggcattgctg aagcccagtt cgtatgcttc tttttcaaag attttgaact gctcgggcgt
     9721 aacgtagcgc aggacgggca ggtgtccgtc tgaaggctgg aggtactgtc cgatggtaat
     9781 catttcgata ttgtgcgccc gcatatcgcg cataatttca cgcacgtctt cgtctgtttc
     9841 gcccaagccg accatgatgc cggatttggt cgggatatgc ggcatcattt ctttataacg
     9901 ttttaataag tctaaagaat gttgataatt agcaccggga cgggcttttc tgtacaggct
     9961 cggatgggtt tctaggttgt ggttcatcac gtcgggcggg gtttcggcaa ggattttgag
    10021 tgcgatgtcc aagcgtcctc ggaagtcggg gacgaggatt tcgattttgg tgttcgggct
    10081 ggtttcgcgg atggctttga tgcagtcggc gaaatgctgt gcgccgccgt cgcgcaggtc
    10141 gtcgcggtcg acggaggtga tgacgacgta acgcaggttc atggctttga cggattcggc
    10201 gaggtttctc ggttcgtcgg ggtcgagcat attgggccga ccgtgtccca cgtcgcagaa
    10261 cgggcagcgg cgggtgcaga tgtcgcccat aatcatgaag gtcgccgtgc ctttgctgaa
    10321 gcattcgccg atgttggggc aggaggcttc ctcgcaaacg gtgtgcatct tttgttcgcg
    10381 caaaatgtct ttgatttcaa agaatttgcg cgatgggagt ttggcgcgta tccattcggg
    10441 ctttttcagt ttttcctgaa gggggacgac tttgatgggg atgcgcgcgg ttttgtccgc
    10501 gcctctgagt ttgatgccgc gtttggggtc gtcggttttg atttcactca ttgttgtctg
    10561 ctttcggtgt gaattgtgtt tcaaggtgtg cggtaagttt ggcggcgact tcgtccggcg
    10621 tggggcaggg ttggacaaaa tccgcgattt gcgtcatttc cataccggcg tagccgcagg
    10681 ggttgatgtg ggtaaacggg cttaaatcca tattgacgtt gagcgcaagc ccgtgataga
    10741 cggagccgtt tttgatacgc agccccagtg aggcgatttt gcgttctccg acataaacgc
    10801 cggggcgttt ggggtctgcc gccgcttcga tgccgtattc tgccaatgtg gcgatgatgc
    10861 tgttttcaag cgcggaaacg atgtttctaa cactggtttt gcgccgtttg aaatcaatca
    10921 tcgtataaac gaccaattgc ccgggcccgt gataggtaat ctgcccgccc cggtcgattt
    10981 ggacgacggg aatgtcgtcg cgaatcagca ggtgttcggg ttttcccgcc agtccttgtg
    11041 tgaacacggg cgggtgttcg acgacccaca gttcgtcttc ggtgtcggca ttccgtccgg
    11101 cattaaaggt tttcatcgct tcaaaagtcg gcagatattc gaccaaacct ttgtgtatga
    11161 ctttcatctc aaagtaccac tttgaccagt tcgtgcgaag tcagcgcgcg gtagatgttg
    11221 tccaattgtt cttggttttc aacctttatc tgaacggtgg cgccggtata gttgcctttg
    11281 ctgctcggac gcgtggtgat gtggtgcgcc tgcgtgtcgg gggcgtggag gcggacggtg
    11341 tctaaaaccg cctgctcgaa ctcgggatgc accgcgccca ttacttttaa tgagaaggtg
    11401 cagggaaatt cgatgaggga tgttttgttt ttttgttcgg tcatgatgtg ctgccttatc
    11461 gtgtacggta tgccgtctga aggcgggttt gcctttcaga cggcatcgga tgtgcgttat
    11521 tttagcctaa accgcgataa caggctatcg ggaaggcgga ggcttttttg acggcgcggc
    11581 ggttctgcta tactggcgcg caatattatt tttagaaggg tggtttttat gtatcggagg
    11641 aaagggcggg gcatcaagcc gtggatgggt gccggtgcgg cgtttgccgc cttggtttgg
    11701 ctggctttcg cacttggcga cacactggct ccgtttgcgg ttgcggcagt gttggcgtat
    11761 gtgttggatc ctttggtcga atggttgcag aaaaagggtt tgaaccgtgc atccgcttcg
    11821 atgtctgtga tggtgttttc cttgattttg ttgttggcat tattgttgat tattgtccct
    11881 atgctggtcg ggcagttcaa caatttggca tcgcgcctgc cccaattaat cggttttatg
    11941 cagaacacgc tgctgccgtg gttgaaaaat acaatcggcg gatatgtgga aatcgatcag
    12001 gcatctatta ttgcgtggct tcaggcgcat acgggtgagc tgagcaacac gcttaaggcg
    12061 tggtttcccg ttttgatgag gcagggcagc aatattgtca gcagtattgg caacctgctg
    12121 ctgcttccct tgctgcttta ctatttcctg ctggattggc agcggtggtc gtgcggcatt
    12181 gccaaactgg ttccgaggcg ttttgccggt gcttatacgc gcattacagg caatttgaac
    12241 gaggtattgg gcgaattttt gcgcgggcag cttctggtga tgctgattat gggtttggtt
    12301 tacggcttgg ggctggtgct ggtcgggctg gattcggggt ttgcaatcgg tatggttgcc
    12361 ggtattttgg tttttgttcc ctatttgggc gcgtttacag gactgctgct ggcaaccgtc
    12421 gccgccttgc tccagttcgg ttcgtggaac ggcatcctat cggtttgggc ggtttttgcc
    12481 gtaggacagt ttctcgaaag ttttttcatt acgccgaaaa tcgtaggaga ccgtatcggg
    12541 ctgtcgccgt tttgggttat cttttcgctg atggcgttcg gacaactgat gggctttgtc
    12601 ggaatgttgg cgggattgcc tttggccgcc gtaaccttgg tcttgcttcg cgagggcgtg
    12661 cagaaatatt ttgccggcag tttttaccgg ggcaggtagg cggttccgaa acatatttga
    12721 agcggaatac aaccttgccc gggtttaaat aaaaatgccg tctgaacccc gaaaacggga
    12781 cttcagacgg cattttcatc acggcttatt tggcggtttt gctgctgtcg ataattttca
    12841 taccggcaga aatcaggcta ccaatgtcgg caacattggc gggcataatc agcgtattgc
    12901 tttctttggc aagattgttg aacgcggcga cgtattgttc cgcaatcttc agattgaccg
    12961 catccgcgcc gccttgggtt tgaagggcgg cggcaatttg acggatggct tcggcattgg
    13021 cttcggcaac aaggcgcaag gattccgctt cacctttggc gcggttgatg cgggcgattt
    13081 tctcggcatt tgacgcattg actgcggctt gggcctcgcc ctcggattgt tggatttcgg
    13141 cttcgcgctg accgctggca aggttgattt gttcgatttt acggccttcg gattcggcga
    13201 tgcgggcgcg tttttcgcgt tcggcggtaa tttgcgcctg cattgagcga aggatttctt
    13261 gcggcggaac caaatccttg atttcgtaac ggagaacttt cacaccccaa gctccggcgg
    13321 cttcatcgag ggcggaaacg acggtgctgt tgatttcgtc gcgttcttca aacgttttgt
    13381 ccaactccat acgcccgata acggagcgca gcgtcgtttg ggcaagctgg gtaatcgcca
    13441 taatgtagtt gctcgaaccg tatgaggcga gtttggggtc ggttacttgg aaatagatga
    13501 tgccgtcaac cgtcagctgc gtattgtcgc gcgtgatgca gacctggctg ggtacgtcta
    13561 aagggatttc tttcagcgaa tggcggtagg cgacgcggtc gataaaggga atcaaaatat
    13621 tcaaaccggc cgtcagggcg cgatggaaac gccccagcct ttcgacaacg tggacttcct
    13681 gttgtgggat gacgacaaag gatttgaagc cgaaaacggc aacggctgcc aacaagataa
    13741 tgaaaaattc cataattcct ccgagtgtta agggtgtgtg ataataagaa ggttaccttc
    13801 cttgcggacg attagagcgc gcgttcccgg ttcaaacaca tcctgtcccg tattttgcgc
    13861 ctgccagtgc gtaccgcgat aaaaaacttc gtaacggttg ccgcctgtgt gccggagaat
    13921 ttcggcatat tgcccggcat ccaaatcctg atatgaatcc gtttcaactt ttcccccggc
    13981 ggttttggcg tgtacgaacc aaatacccag cgcggaaagt agggcggcgg ccaagacggc
    14041 ggcaggcgtg ctgccggtca gtccgtagat aatgcccgaa cccgccaaag ccgcgctgac
    14101 aaccaaaaga taaaccgttc ccgtcaataa ttcgatgatt aagacggcaa cagcagcaac
    14161 aaaccataca gtcatacatt tccccacaaa gcgtgtcgtt tgacaaaatc acgcgatatc
    14221 agcagtatag ccgaatttga aaggataggg cagatatgga cacttggcac gatgcactcg
    14281 gcggcgaaaa acagcagccg tattttcagg aaattttaaa tgcggtccgg caggaacgtt
    14341 tgtcgggaca aatcatctat ccgccggaga cagatgtgtt caacgcattc cgcctgacag
    14401 cgttcgaccg ggtcaaagtc gttattctcg gacaagaccc gtatcacggg gcagggcagg
    14461 cgcacggttt ggcattttcc gtccggcagg gtatccgcat accgccgtct ttactcaata
    14521 tctacaagga gttggaaacc gacatcgaag gcttttccat tcccgcgcac ggctgtctga
    14581 cagcgtgggc ggagcagggc gtattgcttc tgaacacggt tttgaccgta cgcgaaggac
    14641 aggcgcattc gcacgccctt ttaggctggg aacgctttac cgataccgtc atcaggcagc
    14701 ttgcgacaca ccgcaagcat cttgtcttta tgctgtgggg tgggtatgca caacaaaaaa
    14761 ggaaactgat agacagtcaa aaccatttga tattgaccgc accgcatccg tctcctctgt
    14821 cggcatatcg cggttttttc ggctgccgcc atttttcaca ggcaaacagc tatttgagcc
    14881 ggcacggtat cgatccgata aactggaagc tgtgaatgcc atctgaatgc caagttgctt
    14941 aaaccccact gaaggctgct tttgaaaccg gaaacaaagt gattaaggaa aaccggccca
    15001 taatgcaaaa ccgtctgaat cctagggttc agacggcatt ttttgccgcc atttccaaaa
    15061 acaacttttg ttcgcctgcc gacaaatccg acaaagtcgt ttggttgagg gctttggcaa
    15121 taatttaaca taatatacat tatgcgaact atcggaaaca gttgtacgtg tccctgtggt
    15181 ctttccaagt aggaaaatta aagtatggac aatgcggctg aatgtatagc ccggagcatc
    15241 cgcgtatccg aaggtactga acgatatgcc ctgcatccct ttcgatgctt ccatccagcc
    15301 ggtattggga aatacgttga ccttcaatat aatatacagg cagccgacac acaggatatg
    15361 ccgctgcttt ttcatcattt cttctgtcaa atccttggaa cggtcgattt gaaaacacgg
    15421 ctttacatac gtcccaattc tctttgcagt gcttggatat tttgctgcct gtccaataca
    15481 ttgctttgta atgcatttat ttcttgatgg ttgatgttgc cgccctttgc cagacgtgct
    15541 tgtgataaca ttttttgggc ttcagtcagg gctttgcgtt cgttgctcaa ttctgcttcg
    15601 agaatggagc gtctgctgtt gtttacaggt gcttgttgag gcgggggcgt attggatttt
    15661 gccggcttgg atactgtttt gaccggggct ttatatttga cagcctgtcc gccgtttgac
    15721 ggtgatggtg tcggttcgga tgtttggggc gggatatagc gttcgctgct gtagttgccg
    15781 attgggggca aatcggttga gaggcagctt ttggacggct tggtggtgta aacggtttct
    15841 ccgttgattg tgcaggtgta gattttggcc gcattcgcac ccaatgggct tgaaatcagg
    15901 gaaaagttga ttaggattaa ggggagtttt gatttcataa tgtgcttgat gctcggataa
    15961 ttatttgatt ttttggtatt ttttgtaatg ctttcttggc gttttacggg cttttctttt
    16021 cggttgtttg cttttgattt tctttcgggg ctttggcagc ctgcttgttc tttttcccgg
    16081 caggtttttt gtttttcttt ttactttcta tgtgtatttt ggcttcttaa ctgagttttt
    16141 taattttcag gcggtatccg cctccctgat ggctgctgat tttaggtaaa tccgcatcgg
    16201 cgcacgatcc tgctggggcc gatgtatata cgcttttgcc atttgagttg caatggtata
    16261 cggaggcttg cgccgccgcg ccggaaaggg acaggagacc caaggcggca aaaagtctga
    16321 tgtttatacc ggtaaaaggc ggtgataacg cccgaattat accgttattg gcaggcaaag
    16381 ataagcaccc tgtccgcgct tctttatcgc tcggcaaact gtttctcgat ttcagtttca
    16441 atccgttcca attcttcttg acaagccagc caaacctctt cgatttgggc aagttgtgtt
    16501 ttgacttttg ccagcaggga taaggtattt tgcaattttt ctttgtttgc ttcaaggtaa
    16561 gcgtcttctt gtgctaaaaa tgcttcacat gccgtctgaa tttcggaaag ctgcgccatt
    16621 tctttttcgg cacggtctat tttctgctgt atcggcttgc cgcgtcgggc tttttcctga
    16681 cggatttgcg cttcgatgcg cttggtgtct ttgcggcttt ggctttgtgc ggatgctgcg
    16741 ggtgcggcgg cggcgttttc ctgtgccaaa cgccattggc ggtaatcgtt caaatcgccg
    16801 tcgaagttct tcagacggcc tttgtcgatc aggaggaagc tgtcggtagt cgcttcaagc
    16861 aggctgcggt cgtgcgatac ggcgattaat gcgccttgga aactttgcaa cgcgagcgtt
    16921 aaggcgtggc gcatatccaa gtccaaatgg ttggtcggtt cgtcaagcag caacaggttc
    16981 ggcttttgcc agataatcat ggcaagggca aggcgggctt tttctccgcc ggaaaaaggt
    17041 tcggttttct gtaacgccat atcgccaaca aaattgaagc cgccgaggaa atttcggatt
    17101 tcttgttcgc gtacttcggg agaaagctgc tgaatatgcc aaacagggtt ttggtcggcg
    17161 cggatggtgt cgagttggtg ttgggcaaaa tagccgatat tgagtttttc ggaacggatg
    17221 atgctgccgg aaagcaaatc gattttgcct gccaaggctt tgataaaggt ggatttgccg
    17281 ctgccgttga cacccaatag cccgtagcgt gcgccgcttt ccagcgacag ggtaatgtcg
    17341 tgtaggacag ttttgccttc gtaacccaaa tcagcgtgtt ctagctttaa caaaggattg
    17401 ggcagatggt cgggatggta aaactcaaag gaaaactcgc tgtccagatg cgcgggcgca
    17461 acgcgttcta gctttgccaa agccttcatg cggctttgcg cttgaacggc tttggtggct
    17521 ttggctttga agcggtcgat aaaggattgc aaatgtttga tttgcgcctg ctgtttgaca
    17581 taggcagctt gttgttgcgc gagacgctgc gcgcgttcgt tttggtaaaa atcgtaattg
    17641 ccgccgtatt gcgtgaattt ttgctgcgat aattcaatgg tttgggtagt tgccgcgttg
    17701 agaaaatcgc ggtcgtggga aatgatgatt tgcgtgcagg gtaaagaagc aagatggttt
    17761 tccagccaca agacggtttc caaatccaag tggttggtcg gttcgtccaa aagcagcaaa
    17821 tcggcgcggc aaatcagggc ttgggcgaga ttgaggcgca tacgccagcc gccggaaaag
    17881 gatttgacgg ggcggctgtg ttcttcttgc gaaaaaccca gcccgttcaa caattttgcc
    17941 gcgcgggccg gcgcggtata agcgtcgatt tcttccaatt tggcatgata ttccgcctgc
    18001 ttcatgccgt cattttgcgc ttctgcctgc ctcaatgccg tctgaaaagc ctgcaactcg
    18061 gcatcgccct gcaaaacgta atccaaagcg gaaatatcca aatcgggcgt ttcttgggaa
    18121 acggaagcga gccgccagtt tttcggaatc gagacatcgc cgccgtcctg agtgatttca
    18181 cccttgatta aggcaaacag gctcgatttg cccgttccgt ttttgccgat caaaccgacg
    18241 cgctgaccgg gattgacggt agcgttggct ttgtcgagca ggactttcaa accgcgttgc
    18301 agggtgaggt ttttgatttc aatcataacg gaaacatcgt cgggcgggaa aagcccgtat
    18361 tttacctgaa agtcagtgcc gatgccgtct gaaacgggaa atttacggct gaagccaagc
    18421 ccaagccctg cgcccttccg agtgcaggaa aaccaatgtc ctgaatgccg aatcggtatt
    18481 catgcattcc acgctgattc cgattcggga aaaatccgcc atgattttgg gatggataaa
    18541 ctcctgagcc gcgcccgtcc cgataatcaa tatttccgga tagtcaacag gtttgatgtc
    18601 ggacaacagg ttttccggag tcagatcgga caaggttcgg cattgcgaca ggcagaccga
    18661 atccttatgt acaagcacgg gtttatggaa actttgcccc gccagccgga ttccgcccgc
    18721 accgcattca tattccgcaa actgtccgtc tatcggattt tcttcaaaca acattttttt
    18781 accccgtcgc cgcatcatct acaccgaaag gggtgcaaaa tcagacaaat tcatgtagga
    18841 ttggcagatt tcatctgact cgcctgccga tttcagacgg catttgattc aaagtgcggc
    18901 accattatat cggcagcgga tattttcgtc tttcaatatt tacatttcag tcggcttaca
    18961 aggagacaca atgaagccag taaacatcgg tcttttaggt ttgggtacgg tcggcggcgg
    19021 tacggctgcc gtgttgcggg acaacgcgga ggaaatttcc cgtcgcttgg ggcgcgaaat
    19081 ccgtatttct gccgtatgcg acttgagcga agaaaaagcc cgccaaatct gcccgtccgc
    19141 agcctttgtc aaagatccgt tcgaactggt cgcgcgtaaa gacgtcgatg tcgtcgtcga
    19201 attgttcggc ggcacgggca ttgccaaaga tgcggtgttg aaagccattg aaaacggcaa
    19261 acacatcgtt accgccaaca aaaaactgct cgccgaatac ggcaacgaaa tcttcccgct
    19321 ggcggaagaa aaaaatgtca tggtgcaatt tgaagcggca gtagcgggcg gtatcccaat
    19381 catcaaagcc ctgcgcgaag gtttggcggc aaacaggatt aaatccatcg ccggcattat
    19441 taacggcacc agcaacttca tcctctccga aatgcgtgaa aaaggcagcg cgtttgccga
    19501 tgtactgaaa gaagcgcagg cactgggcta tgccgaagcc gacccgacct tcgacatcga
    19561 aggacatgac gcgggccata aaatcaccat catgagcgcg ctggcattcg gcacgccgat
    19621 gaacttttcc gcctgctacc tcgaaggcat cagcaaactc gacagccgcg acatcaaata
    19681 cgccgaagaa cttggctacc gcatcaaact gttgggcatc acccgcaaaa ccggcaaagg
    19741 catcgagctg cgcgtccacc ctaccctgat tcccgaaagc cgcctcttgg caaacgtcaa
    19801 cggcgtgatg aacgccgtgc gcgtcaacgc cgatatggtt ggcgaaacct tatattacgg
    19861 cgcgggcgcg ggcgcattgc cgaccgcttc cgccgtggtt gccgatatca tcgacatcgc
    19921 ccgcctggtt gaagccgata ccgcccaccg cgtaccgcat ctggcgttcc aacccgcgca
    19981 agtccaagcg caaaccatcc tgcctatgga cgaaattacc agcagctact acctgcgcgt
    20041 ccaagccaaa gacgaaccgg gcacgctggg gcaaatcgcc gcgctgttgg cacaagaaaa
    20101 cgtgtccatc gaagcactga ttcaaaaagg cgtgattgat cagaccactg ccgaaatcgt
    20161 gattctgacc cacagcacgg tcgaaaaaca catcaagtcg gcaatcgcag ccatcgaagc
    20221 actggattgc gtggaaaaac cgattaccat gatccgcatg gaaagcctgc atgactgagc
    20281 cgaaacacga aatgccgacg gaagagcagg ttgccgcgcg caaaaaagca aaagccaaaa
    20341 tccgcaccat ccgcatttgg gcatgggtca ttttggcgtt gctcgcttca accgccctgc
    20401 tctcccaatg cgcgatgtcc aaaccgcagg caaaacagaa aattgtcgag tcttgcgtga
    20461 agaatattcc gtttgccgaa aaatggcaaa acgatttacg ggcccgcggt ttagattcaa
    20521 acaatacccg ccttaccgtc gactactgca aatgtatgtg ggagcagcct ttggacagat
    20581 tgagcgagaa acagattaga tccttcggca aactcggcgc acaagaacag cttgacctgc
    20641 tcggcggcgc aaatgccttt gaagcacgtg acaagcagtg tgttgccgat ttgaaatcag
    20701 aataatgtgg accgataaaa aagccgattc tttaaagaat cggctttttt cataaaaaac
    20761 ggcttacagt gcgtctttca aagctttgcc ggcgcggaat ttaggcgttt tggcggcggc
    20821 aatggtcaga ggctcgccgg ttttggggtt gcggccttgg cgttccgcac gttcgcccac
    20881 gtagaaagta ccgaaaccga ccaaagtaac ggtgtcgcct tgtttcaggg cggtggttac
    20941 tgcattggta gtggcatcca aagctttttg tgcggcggct ttggaaatgt cggcttcttg
    21001 agcaatcgct tcgatcaatt cagacttgtt cacaatcagt cccttcctgt cttaaaaaat
    21061 gatgaaatgc ccgaatactc ggggtttgta ctgcttgagc aactttcgct ttatagcaat
    21121 tctgaaattg ccgtgtcaag caaaaaatac ggaatcaccc tatttgacag gctttcagga
    21181 cgaaaccgca tttttacaac acatttcctg cgtttcaatg tttggttgcc ctgctgcggg
    21241 gttttggttt tgaagcggat tccgccgccg cttccgcacc agaaggttct gcccaaggct
    21301 caggctggct ttccaaaccc agagccaata cctcgtctat ccatttgacc ggatggatgg
    21361 tcaggccggt tttcacgttt tcagggattt cttccaagtc tttgaggttg tcttttggaa
    21421 ttaaaacgtg tttgatgccg ccgcgaagcg cagccaacag tttttccttc agaccgccaa
    21481 tcggcaggac ttcgccgcgc agggtaattt cgcccgtcat ggcaacatcg gcgcgcaccg
    21541 ggattttggt gaatgcagac accatcgcca aagtcatggc aatgcccgca ctcggaccgt
    21601 ctttcggcgt cgcaccttcg ggaacatgga catggatgtc ttttttctcg taaaaatcag
    21661 gagccaagcc cactgattcc gcgcgggagc ggacaaccga ccacgctgca gacacggatt
    21721 ccttcatcac atcgcccaac tggccggtgc actgaatcac gcccttaccc ggcaatgctg
    21781 cggcttcgac ggtcagcagt tcgccgccga cttctgtcca cgccagaccg gttacttgtc
    21841 cgatacggtt ttcgctttcg gcaacgccgt aatcgaagcg gcgcacaccc aaatagtcgt
    21901 gcagattttt ctcatttact ttaaccgctt taggtttggc tttgctggtt ttcttggttt
    21961 cagacaacct cttcttatct tcgtccaagg taatctgcat caccaccttg cggcagattt
    22021 tggcaatctc gcggtcgagc gaacgcacgc ccgcctcgcg ggtgtagtaa cggataatat
    22081 cgcgcaccgc gctttcttcg attgccaatt ccccttcttt cacgccgttg cgcttcattt
    22141 gcttcggcac gaggtactgc atcgcgatat tgattttttc gtcttcggtg tagccggaca
    22201 gacggatgat ttccatacgg tcgagcagcg gggtcgggat attcaggctg tttgaagtcg
    22261 caataaacat cacatcgctc aaatcgtaat ccacttcggc ataatgatcg gcgaacttgt
    22321 tgttttgctc gggatcaagc acttcgagca acgcgctggc gggatcgccc cggaagtcgc
    22381 tacccaattt gtcgatttcg tcgagcagga acaatgggtt ttttacgccg gcttttgcca
    22441 tattctgcag aatcttacca ggcatagagc cgatataggt gcggcggtgt ccccggattt
    22501 cgctttcgtc gcgcacgccg cccaaagcca tgcggacata tttccgcccc gttgctttgg
    22561 cgatggattc gcccaaagag gttttgccca cgcccggagg accgaccagg cacagaatcg
    22621 ggcctttgag tttgtccata cgtttttgga cggcgaggta ttccaaaatc cgttctttga
    22681 ctttttccag gccgtagtgg tcggcatcca gcaccagtcc ggctttggcg atgtctttgc
    22741 tgacgcggga ttttttcttc cacggcagtt cgagcagggt gtcgatgtag ttgcgtacga
    22801 cggtggattc ggcagacatc ggcggcatca ttttgagttt tttcagttcg gacaggcatt
    22861 tttcttccgc ttctttgctc atccccgcct ttttgatgcc tgcttccaag gcatccagtt
    22921 cgccgttttc gtcttcttcg cccaattctt tgtgtatcgc tttaatctgt tcgttcagat
    22981 aatattcgcg ctgggatttt tccatttggc gtttgacgcg tccgcgtatg cgtttttcgg
    23041 cctgcataat gtcgagttcg gattccagct gtgccagcag gaattccatc cgtttgccga
    23101 tttcgggaat ttccaaaatc tgttggcgtt gcgccagttt caactgcaaa tgcgctgcga
    23161 ccgtatcggt tagccggctg ttttcggcaa tgccgttgat gctgccgata atttcggcgg
    23221 ggattttttt attgagtttg gcgtattgtt caaactgcgc caacagggtg cggcgcacgg
    23281 cttcgaggtc ggtattgccg cccgtgtctt cttccacgac cgcctctata tgggaaacga
    23341 acagaccgcc cgtgtcttcg atggtcagaa cacgtccgcg atacagacct tcgaccaata
    23401 cttttaccgt gccgtccggc agtttcaata cctgtaagac ttgtgcgacg gtaccggtct
    23461 gatacaggtc ggcggcaacc ggttcttcta gcgccgcatc ggtttgtgcc aataggaaaa
    23521 ccggttcctc gcgggtaatg gcgttttcca gtgcggcgat ggatttcggc ctgccgacaa
    23581 acagcggcag aaccatatgc gggtaaacga cgacatcccg caaaggaagg gttgccaagg
    23641 cggcatattc ctcaaaatgc ttttcttttt gtgtcatagg tactctcttg tgtctgacag
    23701 attgccgatt ttcgcgtaca ttggggttga aggtattatt tcaagcatat gtggtttatt
    23761 tatggagttt gatgcgatgc cgtctgaaac attccggctt cagactgcat gggcttggaa
    23821 agacaaggcg ggaacaaaaa actgttctgt gttgccgctc cttgctgtac catccgtatg
    23881 gtttacggtt ctgccgccct attttcaaaa catgacgcag gtatcccatg tcttctttta
    23941 tttttcccga taacggggcc gaatggcgca acgaaagtgt gcaaaagccg cccaaaggtt
    24001 ggcattatgt ccgcgaaagc ggtgcagacg gcattttgaa ggctgcctat caaaatattg
    24061 caactgtctg gcagggcgat ttccacaatg ccaaacaggt gctttctgca atgaagaaga
    24121 gggctcgcaa acctgccgcc gtccgttccg atgcggatat cgccgccctt ttccatgccc
    24181 accgtatgaa gcaggcgcag cagagccgta ttctgaatat gcttgccgtt gaaatccgcc
    24241 ccggttttgt gttggacaac aaacgcgcgc ccgatatacg cgccgccctg ctcgacgtgt
    24301 acggagaggc ggacggcaaa ccgtttttcc tgccgctcaa tctgctgctg gggtttatgg
    24361 gtgcgcacga gtggcataag aaaggggttg ccgttccgca gctgggcggc agcatacacg
    24421 ttcctttcgg cgtattctcg ccgttgcgcg gcgaatacct cgacctgctc gcccatgcgc
    24481 cgtcaacggg ttttcagacg gcattcgata tcgggacggg ctcgggcgtg cttgccgcca
    24541 ttttggcgaa acagggcatt ccttccgtca tcggcacgga taccaatccg aaagccgtcg
    24601 cctgcgcccg tgccaatatt gcccgtttgg gctttgaaaa acaggttgag atacgggaaa
    24661 ccgatctgtt tcccgaaggg tttgccgatc tgattgtctg caatccaccc tggcttcccg
    24721 ccaagccgac ttccgccgtc gaatccgcgt tatacgaccc cgaatctgcg atgctggctg
    24781 cgtttttgcg ggatgcgccg aaacatctga atcccgacgg agaaatccgc ctgatcattt
    24841 ccgatcttgc cgaacatctg cacctgcgtc cgtccgattt tctggataag gcatttgctc
    24901 aggcgggttt gcgcgttgcg gatgttttga aaaccaaacc ggttcacaaa aaagctgccg
    24961 atccgaacaa tccgctggct tttgcccgaa acagggaaac tactttcctg taccgtttga
    25021 aaaaggcata aggggcggcg gtgcgcttcc ggcggcaggc atcggaacgc ccgcgccgcc
    25081 ggcacggcat cattcgggcg gattattctt gtgaaaatac ccgctcgagc atactgccca
    25141 atgccgtctg acgcgttttg acggtggcgg cagcttgccg gaaggcttct tccccgccga
    25201 agaatacgaa cttctctctc gcgcgggtaa tggcggtata taacagctcc ttactcaatc
    25261 cggacaatgc atcgtcccct tcgtccgaag gtgcggcgga aggcggcagc agccatactt
    25321 cccggtattc cgaaccttgg cttttgtgga cggtcatggc gaatgcgggt tcaaattcgg
    25381 gcaggcagct taccgctacc tttttaaatc cgtccgcatc ggcaaaatag gcggcaaggc
    25441 tgccctgccg tccgacatct tccataatca gtccgatgtc gccgttgaac agttcaagcg
    25501 cgtagtcgtt ctgcctgatc ataatcggct ctccggcaaa atatgccaaa tgttccggta
    25561 tgttcatttt gcggcgtaca tggcggcaat aggcttcgtt gaagtcttcc gcatcctgcc
    25621 gccaagctgc cagaaccacg atatccgaaa tgcccgcgta tgcggcttcg atattgccgt
    25681 cttttaccgc ctgccaatag gctttgtgtg cccggtacaa cctttcgact cgagcgttcg
    25741 gactgcattc cgaatgttcc agttcgtccg gaaaccggtc aaacaatgcc cacgcccctt
    25801 catcgcccga tacggcggca cgggcaaggc agccgatgcc gctgttgtcg ccgaagcggt
    25861 ggctgaacga cagatgggcg gtgttttgcg ccaacacggg tggatttgcg ctgacgctga
    25921 aaccgtgttc cggaaggaag ccggccagcc tttggtgcgt ttctccgtcc aaaacggttt
    25981 tttgtgacaa aacggacagc accgccccta ttccgacgga cgggagctgg ttttcatccc
    26041 ccagcagaat cacgcgcgcg ccggttttga ccgcttttaa aagttgcagc atcaatgccg
    26101 tatccagcat agaggcttca tcgataacca atacgtcaaa cggcagcggg taaacagggt
    26161 tgaacgccgc ctgcattttg ggcgggcgca gcttcagcag tcggtggacg gtctgccctt
    26221 ccagtttgag caaatggcgg cggacggcct ccggcgcgtc aaaaccgttg attgcacggt
    26281 gcagcgcgcg cgccatatgt gccgccgctt tgcccgtcgg tgcggcaagc gcgatgttgg
    26341 gaagattttc gtcttcaccg caaatcagcg ccagcagttt ggcaaccgtt gtcgttttgc
    26401 ccgttcccgg cccgccggta atcaccataa aagactgcaa cagtgccaag gcggcggcat
    26461 cgcgctgccc ttcgctgccc gtgccttgaa accattttgc gaggttttgc ctcgcgcctg
    26521 ccgcgtcggg ggcggacgtg tcggctgccg ccaagcgttt tatctcggca gccaaatcgt
    26581 attccaactg ccacatcctg cccaaaaaca gccttctgcc ttccaaaatc aaaggcgcgg
    26641 cggatgttcc gacaacgggt gcgagtgccg acagcgcgtc agcctcgcca ccgctcaaac
    26701 ggataaacga atgaccgttt tgcaatgcct gaaacaggcg ttcggtgcag tttgcaagca
    26761 cttcgtcgcc ggaacccgca tagtgttcca aaaaacggat tgccgccctt gccgccgctt
    26821 gggcaaattc atctgtctgc cgttccattt tattccttat ccaaatgccg tctgaaggcg
    26881 tggggcttca gacggcgcgg tgttgtttcg gctgtttagg cgtttgcgcc ctgtttgggg
    26941 tgcaggctgc cgtctttcat gaccatcacg cgctcgaagc ggccggcgag ttcgtcgtcg
    27001 tgcgttacga ccaccagccc cgttcccaat tctgttttca gttccagcat catatccaaa
    27061 acattcctgg cgttcgcacg atcgagattg ccggtcggtt cgtcggcaag caggcatttg
    27121 ggttgggtaa ccaaggcgcg tgcaatggcg gcacgctgcc gctcaccgcc tgaaagttcg
    27181 cccgcgcggt gcgtcgaacg gtgtttcagt ccgacctttt ccagcatcgc cattgccgcc
    27241 tccgcagcct cttcacggct ttttttgccg atcagaagcg gcatcattac gttttccagt
    27301 gccgaaaatt caggcagaag atggtggaac tggtacacga aaccgagatg gtggttgcgc
    27361 aggatgccca gttgtcgctg gcttaatgta cgcaaatcct cgcccatcag cagcaccctg
    27421 ccttcagacg gcatatccag cccgcccaaa atatgcagca gcgtcgattt gccgctgccc
    27481 gaagaaccga tgatgccggt gctttccccc gctcggattt ccaaatccaa gccgtgcagc
    27541 acccgaacgt ccaaaccgcc gtcacggtag cgtttgccca cgccttcgca tttcaaaatc
    27601 aactcactca taacgcaaag cctccgccgg ttgggttttt gacgcgcgcc agctcgggta
    27661 gagcgtcgcc acaaaagaca gccccaagga aatgcaggca atcagggcaa cgtcgcccat
    27721 atcgacatcg ctgggcaggt agtcgataaa ataaacctgc gaattgatga ggtggacacc
    27781 gagcaggttt tcaaaaaacg ccacgaccct gccgacgttc caacccaaaa gcacgccgca
    27841 gaccacaccc gccaacgtgc cgaaaaagcc tgaaaacgcg ccctgcacca taaaaatctt
    27901 catcacgccg acaggggaaa gacccaaagt ccgtaaaatc gcaatgtccg cctgcttttc
    27961 cgtaaccgcc atcaccaagg aagagacaag gttgaacgcc gccacggcga taatcagcgt
    28021 caggatgatg aacatcatcc gtttttccaa ctcgaccgcc tcaaaatagc ttcgattgct
    28081 gaacgtccaa tcgcgcaccc aaaccgtatc cctttgcgcc tccggaatca gtgttgccgt
    28141 caaggcgggg gcgttttgcg ggtcggcgag tttcagccgc agccccgcaa cttccttatc
    28201 caagcggtat agtacgcgcg tgtcttggat atgcgtcatc gccaatgagt tgtccacttc
    28261 gtaaacgccc gtcttaacca gaccgaccac ggtaaactgc ttcaggcgcg gcacgactcc
    28321 ggcaggggta acgttgccct ccggcgtgat gacggtaact ttattgccga cttccgcccc
    28381 caaagcctcc gccaagccga caccgaggat aatgtcaaac tcgcccggaa tcaggtcttc
    28441 aaatttgcct gccggcattt tgtcgccgta ttccaccact ttgcgctctt cagacggcaa
    28501 aatgccgcgc atctgcacgc ccctgatttc gcccgcattg gccagcaatg cctgattgga
    28561 aacatagggc gcggcagcca aaataccttt gcggttttcg gtaaaccgaa gcaggttgcg
    28621 ccaatccgta tccgtattgt cgatatagcc gatttcggcg tgcggcgcga cattcaggag
    28681 ctgcccgcgt atttctttct gaaagccgtt cataaccgac aagacgacaa tcagcgcggt
    28741 tacgcccaag gcgattccgg caatcgaaac catcgtgata aacgacataa agccgttgcg
    28801 ctttttcgcc ctgagatacc tcaagcctat ccaagcctct agagaaaaca taacgctacc
    28861 ttaaaaatgt ctgcaaacgt gccgccccgg acggcggttt gggggcggcg gcaaaagttt
    28921 attgtaccgt aaaaccccgg cagcgtccga acgcccgagt gcgggggacg gggcggcaga
    28981 gcgggcggaa aacttgcaca acgcgtcaaa ctgccctatc ctttccttca gaaaaaccgt
    29041 ttcttgagga aaacaatgaa tatccgaact gcttttgctt tgtgcgccat cgccttatcc
    29101 gccgctgcgg ctgcctacgc caaagaaatc aaaatcgatg ccaacaacac gccttattcc
    29161 gaagccgacg cgcaaaagct ggcggcaacg gcagtcggta tgggtgttaa ggaacctatc
    29221 agcctgaacg gcggcagcgg cagcattacc gtgtccggca gcagcgcgac gcagtgcgtg
    29281 ttcaaagtcg gcaacggagg cgcattgcag attcaagggc taaactgcaa gtaaaccgcc
    29341 cggaaaaatg ccgtctgaag gcttcagacg gcattttgca ttggcggcgt tatgccccgc
    29401 cttctttaat caggcggcgt tcgtacaccg cctgcgccag cgttcccgca tcgacatatt
    29461 ccaattcgcc gcccaagggg atgccctgcg acagcctgct gactttgtaa ggcaggtttt
    29521 taaaaaactc ggacaggaca tacgccgtcg cattgccttc tgcggtaaaa gcggttgcaa
    29581 taatgatttc ttcgacttcc ccgccgccca gccgttgcgc cagcctgtcc aatgcgatgg
    29641 cggatacgtc cattcccaat gccgtattga tttgccccat caggacgaaa tacagcccgt
    29701 cgtggcagtt tgccgcttcc atattcgaca cgtcggcagg catatgcacc accatcagcc
    29761 gccgcccgtc gcgtgtttca tcggcacaaa tatcgcacaa tccgccttcg caaaacgtgt
    29821 tgcacatcgc gcaatggtaa acctgcttca atgccgtctg caaggcatcc accagttttt
    29881 cagcctcttt gcgcttgtgt tggagcaaat gatacgctat ccgctgtgcc gatttcggcc
    29941 caacgttggg taaaaccttc agtgcgtcga tcaatccttg gaaggcatct tgttttttgt
    30001 ggctcatcat attccgccgt atgggaaaac ggccggaata ttccgaccgt tattttgtca
    30061 acaaaagtgt caattactga ccgtcgccgt tgtcgaccga ttgcgctcct ttggtctgtt
    30121 tgattttgcc gttgaaataa cgtatcaaca agtcgaaagt attggcagac tgctgttgcg
    30181 ccaaagcctg ttttgcaagc ggaagctgtg cggcgatatc atccggcggg gttacagcct
    30241 gtacttcgac aatcacgggt gccggcagag cgatcagcct gacgtaggcg ggtttgccgt
    30301 ttgccggttt tgctttcagc agttccgcat aagcctcggg cggcatggac tgccttgcct
    30361 gctgtgcgcc caaaacggac acttccgacc atttcacgtc aacagccttg ccgccgttca
    30421 gttgggtaag cacgtctttt gccttgtttt cggcaagttt ggcggcttcg gtacggatat
    30481 aagcctgacg taccgcgtct ttggcttcgg caaacggcag ggttttctct tcgcggactt
    30541 ctttggcgcg gacgacccac gcggtttcgc tgttgatggt cagcacttcg gaattgtgtt
    30601 ttttcttcaa tacgtcgtcg ctgaatacgg cattgatcag gttttcgggc ataccggaca
    30661 tttgcgcatc ctgcctgctc agccaagttt cttgggtttc gactttcaaa ccgctgtttt
    30721 tggcggcttc ggcaagcgag gaaggatggt tgaacgcgtc atcgcccagc ttttcttttg
    30781 ccttattgaa gtcggcaacc gcctttttca ttttcaattc gttttcgacg gcggcttttt
    30841 cctgctcgaa agaaggtttg gcttcatttg tcggcaaacg cgccacgcgc tcttcaaacg
    30901 catttttcac ttccgtttca ctgacggtct gtttgtctgc aaaatccttc agattcaagg
    30961 cgacatattc taatttgacc gcctgcggca gcagatagtc ttttttgttc gcattataaa
    31021 atttctgcaa atcggcttca gacactttga cttgggcgat gaactcgtcg gggttgaaag
    31081 tgtgcgaacg gatggtgcgg ttgacctgcg tcagcctgat cagctgttcc gcctgcgcgt
    31141 cgccgaccaa tacgccgttt tggacgaggt ttaccaaatt ctgcaaggca aactgatcgc
    31201 ggatttcttc gacaaactgg tcttcagaca tatggcgttg ggaaaggtag cggtttaaaa
    31261 gcgcgtggtc gaatttgccg tttgcgtcgt ggaaattggg atcgtccacg ataatctgct
    31321 tgatttgttc ggaagaaacc gaaatgccca tcagcttcgc gccctgtttc aggtaggcgc
    31381 gttgcagcag ggattggaac accgcgtcgc gcgaagggcc gccgccgtcc gcctgttcgt
    31441 tctgtatggc gttgttgatg gagtggtcgc tgattttttc gtcgcccact tggacgatgt
    31501 agtcggcacc cggatgggat accgtgctga ccccgaagcc gacgaaggtt aatgcaatca
    31561 ggcccaaaag gacttgggcg ggcgttctgt atttttcgat ggaatggaac atattttaaa
    31621 tcgggatata gaatgggaac gggaaattca agtcgggtat tgtaacggtt tttatccctg
    31681 tctgcacggg gcttgccggt tgaagatgcc gtcgtaggtt tcttcgctga agccggcgta
    31741 aaccttgccg ccgcactcca atacgggacg cttgatcagg ctcggcattt cggacatcag
    31801 tttgacggcc tccgctgtcg agaacagggc tttttgctgt gtttcggcat cgagtttgcg
    31861 ccagcttgtc ccgcgtttgt tgagcagggt tgccaaaggc acttgttcca gccacgagca
    31921 gatttccgct tcggaaggca cctgtttttt aaaatcccga aattcaaact ccaagccgta
    31981 tccggcaagc cggtttttgg cttttttgac cgtatcgcaa tttgggatgc cgtgaaggac
    32041 tatcatttgg aaaccttttg tctgaaataa taaaacggat attttactat aagtgtctga
    32101 aaatttgccc gtctgtttca gacggcaggg cggttatgtt acaatccgaa aattcgaaaa
    32161 atttaatctc ttgttcaata aaggctttac caatcatgat ttctaccaac ggcatcacca
    32221 tgcagttcgg cgcgaagccg ctgtttgaaa acgtatccgt taaattcggc gaaggcaacc
    32281 gctacggttt gatcggcgcg aacggctcag gcaaatccac cttcatgaaa atcctcggcg
    32341 gcgatttgga acagacggcc ggcgaagtgg cgattgaaaa cggcgtgcgt ttgggtaaat
    32401 tgcgccaaga ccagtttgcc tacgaagaca tgcgcgtgct ggacgtggtg atgatggggc
    32461 ataccgaaat gtgggcggcg atgaccgaac gtgatgcgat ttacgccaat cccgaagcca
    32521 ccgaagacga ctacatgaaa gccgccgaac tggaagccaa gttcgccgaa tacgacggct
    32581 acaccgccga agcgcgtgcc gccgaactgt tgagcggcgt gggcatttcc gaagatttgc
    32641 acaatgcgaa aatggcggaa gtcgccccgg gcttcaaact gcgcgtattg ctggcgcagg
    32701 cgctgttctc caagccggat gtattgctct tggacgaacc gaccaataac ttggacatta
    32761 ataccatccg ctggttggaa ggcgtgttga accaatacga ctccacgatg attatcatca
    32821 gccacgaccg ccactttttg aacgaagtct gcactcacat ggcggatttg gactacaaca
    32881 ccatcaccat ctatccgggc aactacgacg actacatgct cgcctccgcc caatcgcgcg
    32941 aacgcgccct gaaagacaat gccaaagcca aagagaaact gcaagagctg caagagttcg
    33001 tcgcccgctt ctctgccaac aaatccaaag cccgtcaggc aaccagccgt ctgaaacagg
    33061 ccgacaaaat caaatcggag atggtcgaag tcaaaccttc tacccgtcaa aacccgtata
    33121 tccgttttga agccgatgaa aaagccaagt tgcaccgtca ggctgtggaa gtggaaaaac
    33181 tggcgaaacg ctttgaaacc cagttgttta aaaacctgaa cttcatcctt gaagcgggtc
    33241 agcgtctcgc catcatcggt ccgaacggcg cgggcaaatc taccctgctg aaactcttgg
    33301 cgggcgcgta caaccccgaa tattcagacg gcctgctgcc ggacgaaggc agcatcaaat
    33361 gggcggaaaa agccagcgtc ggctactatc cgcaagacca tgaaaacgac ttcgacgtcg
    33421 atatggatct gagcgaatgg atgcgccaat gggggcagga aggcgacgac gaacaagtca
    33481 tccgaggcac tttggggcgt ttgctcttcg gcagtaacga tgtcgtgaaa aaagtgaagg
    33541 ttctctccgg cggagaaaaa ggccgtatgc tttacggcaa actgttgctg ttgaaaccca
    33601 atgtcttggt catggacgaa ccgaccaacc acatggatat ggaaagtatc gaatccttga
    33661 acatggcgct ggaaaaatac aacggcacgc tgattttcgt ctcgcacgac cgtcagttcg
    33721 tttcctcctt ggcaacccaa atcatcgaac tggacggcaa aggcggatat gaacactact
    33781 tgggcgatta cgaaagttac ttggagaaaa aaggcgtagc ataaccgccg gttggaacaa
    33841 tgccgtctga agccgcttca gacggcattg ttgataactt taaaatagga agcatatgca
    33901 gacttacctc gtcggcggcg ccgtccgcga ttatcttttg ggcttgcccg tcaaagaccg
    33961 cgattgggtg gtcgtcggcg cagacgcaca aaccatgctg gcgcaaggct tccagccggt
    34021 cggcaaagat tttcccgtgt ttctccatcc cgaaacgcac gaagaatacg ccctcgcccg
    34081 caccgagcgc aaaaccgcca aaggttacgt cggtttcagt ttccacgccg acaaagacgt
    34141 tacgctggag caggacttga tgcgccgcga cctgaccatc aacgcgatgg cgcaagatgc
    34201 ggacggcaag attatcgacc ctttcggcgg acaacgggat ttggcggcag gcattttgcg
    34261 ccacgtttcc ccggccttcg ccgaagaccc cgtccgcatc ctgcgtactg cccgctttgc
    34321 cgcgcgttac aggtttgaaa tcgccgaaga aaccataaag ctgatgcggc agatggtgga
    34381 aaacggcgaa gcggacgcat tggttgccga acgcgtctgg caggagtttg cgaaaggttt
    34441 gatggaaaaa aatccgcgca aaatgattga agtgttgcgc gaatgcggcg cgctcaaagt
    34501 cttgctgccc gaagtcaatg ccctcttcgg cgtgccgcaa cgcgccgact accatcccga
    34561 aatcgacagc ggcatccata ccctgatgac gctgcaacgc gccgccgata tgggtttgag
    34621 cctgcccgaa cgctatgccg ccctgctgca cgacttgggc aaagccaaaa caccgtccga
    34681 catcctgccg cgccaccacg gacacgacct cgccggtgtc gaacccgtgc gcgaagtcaa
    34741 tcagcggctg cgtgcgccga aacattgcgc cgagcttgcc gaattggttt gccgttggca
    34801 cattattttc caccaagtcg gacagcttaa aagccaaacc attctgaacg tattgaaaaa
    34861 aaccgatgcc ttccgccgtc ccgaacgctt tcagacggca ttgaacgtct gcattgccga
    34921 cacgcaaggc cgtctgaacc gcgaacacac gccctacccg caacgcgcac actggttcgc
    34981 cctgctcgaa gccgccaatc aggcggattc gggcaaaatc gccgccgaat gccgcgcgca
    35041 gggaaaagcg caccttatcg ccgaacaaat cgaccgggcg cggctggcac aaatcgcccc
    35101 attgcaaaaa acgtttcgag cggcgcaaga caaaacagaa aaacattaaa acgtccaatg
    35161 cagcca
//
LOCUS       DAXHTR010000004        33069 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.004, whole genome shotgun sequence.
ACCESSION   DAXHTR010000004 DAXHTR010000000
VERSION     DAXHTR010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33069
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.004"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..228
                     /locus_tag="ACFNML_000133"
     CDS             <1..228
                     /locus_tag="ACFNML_000133"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391595.1"
                     /translation="NPKIQNHRKLSKKQKPSAVVPAKAGIRNRKPQKFIGKTETQKQR
                     KPNGQDSRLRGNDGSYITQKIETKQMGFPLA"
     gene            complement(504..1250)
                     /locus_tag="ACFNML_000134"
     CDS             complement(504..1250)
                     /locus_tag="ACFNML_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6391596.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(1234..1413)
                     /locus_tag="ACFNML_000135"
     CDS             complement(1234..1413)
                     /locus_tag="ACFNML_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391597.1"
                     /translation="MNGKCRLKTARPAFLRRRATAVCRDKKVQAAVFTVKWRIILQRR
                     QFRRFLIRTVYGFSF"
     gene            complement(1429..2100)
                     /locus_tag="ACFNML_000136"
     CDS             complement(1429..2100)
                     /locus_tag="ACFNML_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFC6391598.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(2169..2840)
                     /gene="rpiA"
                     /locus_tag="ACFNML_000137"
     CDS             complement(2169..2840)
                     /gene="rpiA"
                     /locus_tag="ACFNML_000137"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFC6391599.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFICIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2917..3399)
                     /gene="ispF"
                     /locus_tag="ACFNML_000138"
     CDS             complement(2917..3399)
                     /gene="ispF"
                     /locus_tag="ACFNML_000138"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFC6391600.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDEDSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(3431..4120)
                     /gene="ispD"
                     /locus_tag="ACFNML_000139"
     CDS             complement(3431..4120)
                     /gene="ispD"
                     /locus_tag="ACFNML_000139"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFC6391601.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEGVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLAAET
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAIPVADTLKRADGGNISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGVRPLLIQGDVRNLKLTQPQD
                     AYIVRLLLDAV"
     gene            complement(4117..4851)
                     /gene="dnaQ"
                     /locus_tag="ACFNML_000140"
     CDS             complement(4117..4851)
                     /gene="dnaQ"
                     /locus_tag="ACFNML_000140"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFC6391602.1"
                     /translation="MIQRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGKVYLAMTRRQFDLMGEAEEEEPAAKPVASAEMKLGAKLKVIKADEAELAAHG
                     QYLDGLGEACIWRKEAVPSENGGADA"
     gene            complement(5009..6319)
                     /locus_tag="ACFNML_000141"
     CDS             complement(5009..6319)
                     /locus_tag="ACFNML_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6391603.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRYKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMTLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFLFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(6309..6494)
                     /gene="ccoS"
                     /locus_tag="ACFNML_000142"
     CDS             complement(6309..6494)
                     /gene="ccoS"
                     /locus_tag="ACFNML_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HFC6391604.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6494..6793)
                     /locus_tag="ACFNML_000143"
     CDS             complement(6494..6793)
                     /locus_tag="ACFNML_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391605.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVVKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            7144..8340
                     /locus_tag="ACFNML_000144"
     CDS             7144..8340
                     /locus_tag="ACFNML_000144"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6391606.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKGLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTAGSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8976..10817)
                     /locus_tag="ACFNML_000145"
     CDS             complement(8976..10817)
                     /locus_tag="ACFNML_000145"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFC6391607.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGALPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPRAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFALGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANTP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10908..11378
                     /locus_tag="ACFNML_000146"
     CDS             10908..11378
                     /locus_tag="ACFNML_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HFC6391608.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSVLEQKALRIIEAHPEYH
                     RYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKRGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAEDQARLNPHEIA"
     gene            complement(11449..12261)
                     /gene="hpnC"
                     /locus_tag="ACFNML_000147"
     CDS             complement(11449..12261)
                     /gene="hpnC"
                     /locus_tag="ACFNML_000147"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HFC6391609.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLDALRRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYAHFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKRALWK"
     gene            complement(12348..12830)
                     /locus_tag="ACFNML_000148"
     CDS             complement(12348..12830)
                     /locus_tag="ACFNML_000148"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6391610.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDSELVRIEDVGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            13303..13569
                     /locus_tag="ACFNML_000149"
     CDS             13303..13569
                     /locus_tag="ACFNML_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391611.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPATEAPAAEAPAAEAAAT
                     EAPAAEAPTAEAAATEAPAAEAPAAEAAATEAPAAEAPAAEAAK"
     gene            13679..15046
                     /locus_tag="ACFNML_000150"
     CDS             13679..15046
                     /locus_tag="ACFNML_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFC6391612.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLIIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKFPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAVPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            complement(15227..17212)
                     /locus_tag="ACFNML_000151"
     CDS             complement(15227..17212)
                     /locus_tag="ACFNML_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFC6391613.1"
                     /translation="MISLFFSILYTSPLLAVDYVYDNSKLTNEQIDRLKKLRDKSSEY
                     WKQETYLLKPTNGSSQNSLKVPALYSKTHQFKNINNSKKISFYDKEYTEDYLVGFARG
                     FGVEKRNGEEEKTLQQYFKDCVNTGNSNNDKCEQPSFENSGPRLIKSDIFALAPQIKN
                     SHINSEILSVGNYIEWLRPTLNKLPSWQEHLYSGLDPFHYIEVTDNSHVIGQTISLDE
                     FRLENSLWEPRWDSDVGELKTTNADIRFNTKSESLLVKEDYAGGARFRFAYDLKDKVS
                     ETPVLTFEKNITGTSDIIFENPIDDLKSLDGHQIIKVNGTADKHAFRLSGKHQKGIYT
                     LSLQQRPEGFFTKVQERDDISIYAQQAQAANTLFALRLNDKNSDIFDRTLPRKGLWLR
                     VIDGHSNQWVQGKTAPVEGYRKGVQLGGEVFTWQNESNQLSVGLMSGQAEQRSTFRNP
                     DTDNLTTGNVKGFGAGIYATWHQLQDKQTGAYADSWVQYQRFRHRINTEDATERFTSK
                     GITASIEAGYNALLAEHFTKKGNSLRVYLQPQAQLTYLGVNGKFSDSENAHVNLLGSR
                     QLQTRVGVQAKAQFSLYKNIAIEPFAAVNALYHNKPFGVEIDGERRVINNKTAIESQL
                     GVAVKIKSHLTLQASFNRQTSKYHQAKQGALNLQWTF"
     gene            complement(17628..18074)
                     /gene="smpB"
                     /locus_tag="ACFNML_000152"
     CDS             complement(17628..18074)
                     /gene="smpB"
                     /locus_tag="ACFNML_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFC6391614.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(18129..19139)
                     /gene="waaF"
                     /locus_tag="ACFNML_000153"
     CDS             complement(18129..19139)
                     /gene="waaF"
                     /locus_tag="ACFNML_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HFC6391615.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAVIALAT
                     GIAKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPTRHFAELGKHYLAAGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(19270..19345)
                     /locus_tag="ACFNML_000154"
     tRNA            complement(19270..19345)
                     /locus_tag="ACFNML_000154"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19310..19312),aa:Asn,seq:gtt)
     gene            complement(19536..20288)
                     /locus_tag="ACFNML_000155"
     CDS             complement(19536..20288)
                     /locus_tag="ACFNML_000155"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFC6391616.1"
                     /translation="MITLPSLNNLPSKWDEIRHCLETRVFECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEVRYPDSLNELVCAAIATPLGEMLAVFGGKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFQCRLKTFATPLEMI
                     GTAFQKQAWDALLAIPYGETRSYKEQAQHLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            20599..21123
                     /locus_tag="ACFNML_000156"
     CDS             20599..21123
                     /locus_tag="ACFNML_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HFC6391617.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGDNAPPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVRRRN
                     AASLQLSRCASIADDPSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSAASA
                     ALCGTSSSVSTAAA"
     gene            complement(21863..23008)
                     /gene="dapE"
                     /locus_tag="ACFNML_000157"
     CDS             complement(21863..23008)
                     /gene="dapE"
                     /locus_tag="ACFNML_000157"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFC6391618.1"
                     /translation="MTETQSLELAKELISRPSVTPDDQNCQKLLAERLHKIGFAAEEL
                     HFGDTKNIWLRRGTKAPVVCFAGHTDVVPTGPVEKWDSPPFEPAERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNEYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLDDIPKLSAVYEGILARLLAGNAV"
     gene            complement(23122..23742)
                     /locus_tag="ACFNML_000158"
     CDS             complement(23122..23742)
                     /locus_tag="ACFNML_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI_0552 family protein"
                     /protein_id="HFC6391619.1"
                     /translation="MLTPKSCDLFNIPFFQFAQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRRRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     RLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(23776..24285)
                     /locus_tag="ACFNML_000159"
     CDS             complement(23776..24285)
                     /locus_tag="ACFNML_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6391620.1"
                     /translation="MNPFETKSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQIVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(24380..24931)
                     /gene="azu"
                     /locus_tag="ACFNML_000160"
     CDS             complement(24380..24931)
                     /gene="azu"
                     /locus_tag="ACFNML_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HFC6391621.1"
                     /translation="MKTYLALISAAVIGLAACSQEPAAPAAEATHAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKASMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEESSLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(25212..25634)
                     /locus_tag="ACFNML_000161"
     CDS             complement(25212..25634)
                     /locus_tag="ACFNML_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HFC6391622.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLAPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            25765..28515
                     /gene="secA"
                     /locus_tag="ACFNML_000162"
     CDS             25765..28515
                     /gene="secA"
                     /locus_tag="ACFNML_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFC6391623.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHTLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPIQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALENGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            28659..30431
                     /gene="dnaG"
                     /locus_tag="ACFNML_000163"
     CDS             28659..30431
                     /gene="dnaG"
                     /locus_tag="ACFNML_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFC6391624.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMIVPKVRGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPTAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLAQVFQPYPNTALVDTGMVIDNEGRYYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAERILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMPTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAETIKNHPSVPATAQV
                     LEHMRGSPYEETINRIFRSALQSEEMEGGGEEDCENFQIGIKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            30618..32546
                     /gene="rpoD"
                     /locus_tag="ACFNML_000164"
     CDS             30618..32546
                     /gene="rpoD"
                     /locus_tag="ACFNML_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFC6391625.1"
                     /translation="MSKNQNHEEYQDDARPLTIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            32693..>33069
                     /locus_tag="ACFNML_000165"
     CDS             32693..>33069
                     /locus_tag="ACFNML_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6391626.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRIR
                     YVRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINR"
BASE COUNT         8404 a         9403 c         7757 g         7505 t
ORIGIN      
        1 aatccaaaaa tccaaaatca caggaagtta tcaaaaaaac agaaaccatc cgccgtcgtt
       61 cccgcgaaag cgggaatccg gaaccggaaa ccacaaaaat ttatcgggaa aaccgaaact
      121 caaaaacaac ggaaaccgaa cggacaggat tcccgcctgc gcgggaatga cggctcatac
      181 atcacccaaa aaattgaaac caaacagatg ggattccctc ttgcgtgagg ctgacagatg
      241 ccgtctgaaa gactttcaga cggcatagct ttttctcttt gaatttatag tggattaaca
      301 aaaatcagaa caggcgacga agccgcagac agtacaaata gtacggaacc gattcactcg
      361 gggcttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg
      421 tttttgttaa tccacgataa atttgccaca aaaaagctgc ctcaaatgaa tacccgggca
      481 gctttttgtt gatatgactc caatcagcgg tgttgcggat tgtaacgttt ttccaaacgc
      541 aggaatatcc agcctaagaa agtcgtcatc aacagataaa tcagggcgac ggtgtaaagc
      601 ggttcttcat aaaccgaata ccggcccgta atcgtattct gaacatacgc caactccgcc
      661 acagcaatga ccgacagcag cgagctgtct ttcaagagcg tgatgaactc gctcgccaaa
      721 ggcggcagca tgcggcgcaa tgcctgcggc agaatcacat agcgcatcgc ctgcggatag
      781 gtcagcccca aagaacgcgc cgcctccatc tgtcctttgt ctatagactg gatgcccgcg
      841 cggaaaatct cacagatata cgcccccgag ttggcgatca gtgccaaaga accggcaatc
      901 agcggcccgt atccgcgacg cagcgcgatt gccgcctcgc cgctgaccaa aatgccgtct
      961 gaaggatgga cgaaaaacgg aaaccacaca tacgcccaaa tcacaatctg cacaaacagc
     1021 ggcgtacccc ggaacagcgt aacatacagc agcgaaactt tacgcaacgc ccacgccagc
     1081 acgcgcatcg gcgcaccggc tttttccaag tgaatcaggc gcgccaacgc caacaacaga
     1141 cccaataccg aaccgcccgc cgtcgccacg accgtcagcc ccaaggtcgt cagtgcgccg
     1201 taaaggaaca tccagcggta ttcgtaaata atgtcaaaac gaaaatccat ataccgtccg
     1261 tatcaaaaac cggcggaact gccgccgttg caaaataatc cgccatttta ccgtaaaaac
     1321 cgccgcctga acttttttat cgcggcagac ggcggttgcg cgtctccgca aaaatgcagg
     1381 gcgcgcggtt ttcagacggc atttgccgtt caaagccgtg cggtgtcttt accaaatgcc
     1441 caaccattcg cccacggcat ccatccaatc cttattgccc ccgccgctcc tgcctgctcg
     1501 gcggtacgcc cacggcgctt gcggattttt agctttccac aatcctttgc gttccctttc
     1561 cgcctgaatt tgagcgtcgg catagtcggc aaaatccgcc ttatcctgct gttctttagc
     1621 ataactttta taatgccacg ccgccccgtc ctgcacctgc atcaggttca aatcggtttt
     1681 gccgacagaa acctgcgcca cttcgcgctg gtagcggtcg gtgtcgaaca cgcgcacgct
     1741 gactttcctg ccttccgccg ccgcgcgcag gttgtcgcgc gaacgcgtgc cgtaagcctg
     1801 tttcatctcc ggcgcgtcga tatacgccat ccggattttg tgtttcgcgc cgtcgccgtc
     1861 gataacgtga agggtgtcgc cgtcatagac tttggacacc gtgcctgtgt agcggtggcc
     1921 ggatttcgcc gatgctcggc ggcgggcggg cgcgtcggaa cccgcgtccc ctgccgcgcc
     1981 gagtacgtcg agtacggcaa ccgccgtccg caccgcctcg ctgccgtacc ccgtataacc
     2041 caacgcaccc aaaagcgaca gggcgacggg aagccatttc atgatttttt taatctgcat
     2101 atttttcaaa tgccgatgcc gtctgaacat ctctgaaccg gatttcagac ggcatcttaa
     2161 cgtcaggatt acccttggca gggatagatg actttcgcac cctcttccgt ccccaaaatc
     2221 aacacatcgg cggcatcgcg ggcgaatatg ccgttttcga gtacgccggt gattttgttg
     2281 atttcgtctt ccatcatcag cggtttgtcg atattcaagc cgtggacatc gacgatttgg
     2341 ttgccgtaaa acgtggtgta gccgatacgc agttcgggct gtccgcccat agcgagcagt
     2401 ttgcgcgaaa caagggaacg cgcgctttcg acgacttcca ctggcagagg gaatttgccc
     2461 aaacgtgaaa catatttgct ttcatccgca atgcagatga atttttctga tgcgctggcg
     2521 acgatttttt cgttgaggtg cgcgccgccg ccgcctttaa tcatttgcag ggcgtggttc
     2581 acttcatccg caccgtcgat atagaccgcc aaccccgata cttcgttcaa agaaacgacg
     2641 ggaatatcat attgggcaag cagttcgctg gattttttgg aagtagatac cgcgcctttg
     2701 atttttttgc cgctcttacc caaggcttcg atgaaaaagt tgatggtcga gccggtaccg
     2761 atgccgatgt attcgttttc gggtacgaat tcgactgctt tttcggcggc gatgcgcttg
     2821 agttcgtctt gtgtcgtcat atttttgtcc tttgggaaac cgtatcaaca aacagccgtc
     2881 atcttaacat ttttttgcac gtcctgcccg ccgcgttcaa atccgtacca gcaataccgc
     2941 cgcctgcgcc tctatgcctt ccatgcgccc gagataaccg agtttttcgt tggttttgcc
     3001 tttgatgttg acgcacgaaa tgtctatgcc caaatcggcg gcgatgttgg cacgcatttg
     3061 cggaatatgc ggcgcgagtt tgggtttctg tgcaatcacg gtcgtatcga cattgaccgc
     3121 ctgccaaccc tgcgcctgaa cgctttgata cgccgcacgc aaaaggacgc ggctgtcctc
     3181 atctttgaac tctgcggcgg tgtcggggaa atggctgccg atatcgccca aacctgccgc
     3241 accgagcagc gcgtcggtaa cggcgtgcag cagcgcatcg gcatcggagt gtccgagcag
     3301 ccctttttca aatgggattt caactccgcc aagtatcagc tttctgcctt cggtcagttg
     3361 gtggacatcg tagccctgtc cgatacggat gttcgtcatc gtttgtgttc ctgatggttt
     3421 gaattgaagt tcagacggca tcgagcagca gcctgacgat gtatgcgtcc tgcggctgcg
     3481 tcagtttcaa attgcgcacg tcgccctgta tcagtagcgg acgcacaccc aatttttcca
     3541 cggcggacgc ttcatcggta atgccgtcca agttttccgc agccaatgcg cggtgcagca
     3601 gcccggcgcg gaaaagctgc ggcgtttgcg cctgccaaag gctcgtccgc tcgacggttg
     3661 cactaatgtt cccaccgtcc gcgcgcttga gcgtatcggc aacgggaatt gccaaaatcc
     3721 cgccttcggc ggcgttgccc gcctgttcta tcaaacgcgt cagagcttca gacggcaggc
     3781 agcagcgcgc ggcatcgtgt accaaaatat tgtcggtttc cgccgccaaa ccgatttcca
     3841 acagttttgc cacaccgttt cggacggttt cggcgcgggt ctgtccgccg tttttccaca
     3901 cccgaacctg tggaaatgcc gtctgaacct tatcggcaaa cgtgtcttcg ggcgagacga
     3961 caacgacggt caaatcgacg ccctcatgcc gttcaaaaat cccaatcgta tgttctaaaa
     4021 cggttttgct tccgatttcg acatactgct taggtttgtc cgcaccgaaa cgcgccccga
     4081 tgccggcggc gggaatcagc gcgatatttt tgcgcttcat gcgtccgccc cgccgttttc
     4141 cgacggcacg gcttccttgc gccagataca ggcttcgccc aagccgtcca aatattgccc
     4201 gtgcgccgcc agttcggctt catccgcctt aatcactttc agtttggcac cgagtttcat
     4261 ttcggcagat gcaacaggtt tggctgccgg ctcttcctcc tcggcctccc ccatcaggtc
     4321 gaactgccgc cgcgtcatag caagatagac tttgcccaaa agttcgcagt cgatcaatgc
     4381 gccgtgcagg acgcgcttgc tgcggtcgac ggaaaaacgg ttgcacaagg catccaggct
     4441 ggctttctgc ccggggaaca tttcgcgcgc catcgccagg gtatcggtaa cggtacagcc
     4501 gagttcctcg acagtcggca gccccatccg gcggaactcc atattgagga agcccacgtc
     4561 gaatttggca ttgtggataa tcagttccgc accgcgcagg aaatcggcaa tctgcctgcc
     4621 gacctctgca aacggcggcg cgtttttccc ttccaaaacc tctatcgtca gaccgtgcac
     4681 cctcgccgcc tcttcgggca tatcgcgctc ggggtggaca tagaggtgca ggtttttgtc
     4741 ggtcatttgg cggttgacca tttctaaacc ggcaaactcg accaagcggt cgccgccgtc
     4801 ggcatacaga ccggtggttt cggtatcgag gatgatttgg cgttgaatca tgtctgcttc
     4861 tttcttcaaa ttttccatct gctgactatt ttttaagcaa tatatttttc tgttttcatt
     4921 tcaatgcaca aacccactta ttcacagtgt gttcacaaca ttgggcaggc ggattgtgta
     4981 ttttggggac aattttttca gacggcattc aaggtttttt cctgattgcc gccgcgccta
     5041 aaaaccgcct ttcgcgctta atcaaaaata ccgacaacgg aatattgccc aaagcgacaa
     5101 tcagatacaa caaggaaatg ctgtcaaaca aaaacagcaa caccgcgctc aaaacggcgg
     5161 cggaaaccat aaaaataccg ttaacgatat tgttggcggc aacggcgcgg gcgcggaaag
     5221 tctcgctact ggcggtttgc agccaagtat agagcggaac ggagaaaaat ccgccgaaaa
     5281 agccgatcag cgtcatcacc gccatcacgg gatatgccca tccttgcgat aaaaaccaaa
     5341 aaatgccgtt cagcccttca aaacggtgtc cgtgcgtcag ccacaccaaa accaggccgc
     5401 aaaccgtcaa acccaacgca ccaaccgtta cccaagccaa catcagccgt tccctgctga
     5461 acttggcaca cagtaccgaa ccggcggcaa taccgatgga aaacagggca agcatcaggt
     5521 tgaaaacatt gtcgttgccg cccaaatgga tttgggtaaa ggtcggcagt tgcgtagtat
     5581 agaccgcgcc gacaaaccaa aaccacgaaa taccgataat agcggtaaag acgggtttgt
     5641 accgcaccgt ttcacgaagc agggattttg tgccacggac aatattccac tcaatttggg
     5701 tatcggcagc cttggcgggg acggacggca taaacaggct gccgaccgtg cctccgacgg
     5761 cgaccagcaa aaccagtatc ccgacaatat aaggcggtac acctgccacc gccgttccca
     5821 aaatctgacc gaacaggatg gcgacaaacg tacccgattc aatcaggctg ttgcccatca
     5881 tcaactcttt gtcgtcgaga taatcgggca ggatggcgta tttcagcggc ccgaacagcg
     5941 tcgattgcgc gcccatgcaa aacagacacg ccaaaagcag cggggcagac cggatataaa
     6001 acccgtatgc cgccaccgcc ataatgatca tttccagcac cttgacccaa cgcgccaaaa
     6061 cggccttgtc gaatttgtta cccaactgcc ccgacagcga ggaaaacagg aaatacggca
     6121 aaataaacaa caacgcgccc aagttcaaca tctgtccggc aggcaggaag ccgttttgcc
     6181 ccaaaccgta aaacccaatc atcacaaaca gcgcggtttt gaacacattg tcgttgaacg
     6241 cgccgagaaa ctgcgtagcg aaaagaggtg cgaaacggcg gcttttaacc agtcccaaac
     6301 cgcctttttt agcgtacatc gttttccctc tctttttcaa tcagtttact tgtcgaatca
     6361 tcatccatca ggatgcggtg cgccggccct tccaagtcgt caaactgccc gtttttgccc
     6421 gaccaccaaa aaaaccagcc gatgacaaac gccaaaataa tgctgatggg caccaatata
     6481 aacatgcttt ccatcacata ttccctgtca aatcgttcaa aacaaaagtc tgccccgaca
     6541 cggtcagata ttcgttacgc aaagttccga cgggagcttc gtcaaaaaac agctcgatac
     6601 ggtctttgac cacgcgccaa tattggggaa tttccgtctg accgaacggc gacaggacat
     6661 gattttccat tccgccttca agtttgacgg caaaacgccc gctttgcggc cgtgcttccg
     6721 attcgtcgtc ggcaagcagg atgaaaaagc ctatatgccg tcccgattgg tcatgaatac
     6781 tgaaataatg cataaatttc ccacccgcct tttttcagac gacaccaact aaaaacaggg
     6841 cgaatgtacc agtttggacg ggaagaatgc aaagaaattc tccctccccc agccgaaaac
     6901 accggcaaac cgcatatccc ccttttttcc gtcaaaatac ccgacttccg ccattttcac
     6961 gcaaacgccc gattaagcca agcaattgca aagatttttt gttagaatag cctgcttctt
     7021 ttatcaaccg tttcagacgg ccccactact ttcccgccta ggaaggcaaa acggattcgg
     7081 cacgaatccg gttagtatcc gtgtccgatt ccaatgccgt ctgaaacttt ccggagtaag
     7141 aaaatgtccc aaaaattgat cttggttttg aactgcggca gctcgtccct caaaggcgcg
     7201 gtcctggata acggcagcgg cgaagtcctg ctcagctgcc ttgccgaaaa actcaacctg
     7261 cccgatgcct acatcacatt caaagtaaac ggcgaaaaac acaaagtcga tctgtccgca
     7321 catcccgacc acaccggcgc ggtcgaagcc ctgatggaag aactcaaagc ccacggcctc
     7381 gacagccgca tcggcgccat cggccaccgc gtcgtcagcg gcggcgaact gtacagcgaa
     7441 tccatcctcg ttgacgacga agtcattgcc ggcatcgaaa aatgcatccc gctcgccccc
     7501 ctgcacaacc ccgcccacct cttgggcctg cgtgccgcgc aaagcatttt caaaggcctg
     7561 cccaacgtcg tcgtattcga tacctccttc caccaaacca tgcccgaagt cgcctacaaa
     7621 tacgccgttc cgcaggagtt gtatgaaaaa tacggcctgc gccgttacgg cgcgcacggt
     7681 accagctacc gcttcgtcgc cgacgaaacc gcgcgcttcc tcggcaaaga caaaaaagac
     7741 ctgcgtatgg tcattgccca cttgggcaac ggcgcgtcca tcaccgccgt cgccaacggc
     7801 gaatcgcgcg acaccagtat gggcctgacc ccgctggaag ggctggtaat gggtacgcgc
     7861 agcggcgaca tcgatccttc cgtattcggc ttcctcgccg aaaacgccaa tatgaccatc
     7921 gcccaaatca ctgaaatgct gaataaaaaa tccggtctgc tcggcatttc cggcctgtcc
     7981 aacgactgcc gcaccattga agaagaagcc gccaaggggc ataaaggcgc gaaattggcc
     8041 ttggatatgt ttatctaccg ccttgccaaa tacatcggca gtatggcggt tgccgcaggc
     8101 ggtttggacg cactggtctt taccggcggc atcggcgaaa actccgacat catccgcgaa
     8161 cgcgtgatcg gctacttggg cttcctcggt ctgaacatcg accaagaagc caacctgaag
     8221 gcacgcttcg gcaacgccgg cgtgattacc actgccggca gcaaagccgt tgccgtggtc
     8281 attccgacca acgaggaatt gatgattgca cacgataccg cccgcttgag cggtctgtaa
     8341 gattcaatcc ccacgcgaac tgcctccgaa aacggaggca gtttttttat ccggctttcc
     8401 atgcttaaac agcactgcct cttttcagac attgacggtt gcagccgctt acctgaacct
     8461 tatagtggat taacaaaaac cagtacagcg ttgcctcgcc ttagctcaaa gagaacgatt
     8521 ctctaaggtg ctcaagcacc aagtaaatcg gttccgtact atttgtactg tctgcggctt
     8581 cgtcgccttg tcctgatttt tgttaatcca ctatatccga ctttccatac ttaaacagca
     8641 ctgcctcttt tcagacattg acggttgcag ccgcccacct gaaccttaat gattgactat
     8701 tttttaatca tgttattatt ttccataaaa tacatggcat taagatgttt ttccacaaaa
     8761 gatacacaca ccggcaaaca ccggctgtgt ttatcttttc ttatgcctat tttttaatca
     8821 tcgtattttt atcttttaat ttcaatacgc aaactaactt atacacacgg ttttcacatc
     8881 tttagactgc ttccgtgtgt atagtggata ttgccgtttt cctttctgac aaaaatgccg
     8941 tctgagaact tcagacggca tttgaaacat cggaatcagc ggttttgttc ataccactcg
     9001 ataaacttgt ctgctttgac aaaacccagc agcggctcgc tgcggctgcc gtcggagcgg
     9061 acgacaaaca cgcccggcgg cccgaacaga ccgtattctt tcaacaacgc ctgatgttca
     9121 ggcgtgttgg cggttacgtc gatttggaaa aagcgttcca tatcaaccgc ctgatgcact
     9181 tccggctgat tgagcgtgta tgccgccatt tctttgcagg aaatacacca gtcggcataa
     9241 aaatccaaaa cgacgggttt gtcgggatgt tctttcaacg ccgtatccat cgctgccttc
     9301 agcgcggcag tatcggcaaa cattttgccg tgttccgaag atttgcctgc ttcggctggt
     9361 ggattgaggg tcaggaaatg gtgcagcgcg gtcgttttgc cgtttgcgcc ctgccagccg
     9421 aaccacgcgc cgcctatcag caatatgccg cccaatgcga atgccacagc tttcggacgg
     9481 cgtttctgcc tgcgtccgtt gaccagcagc atacaggcag gaaccagcat cagcagcgtg
     9541 tacagcgcga cgacgagata atagggcaag tgcggcgtgg cgaggtaaac ggcgacggca
     9601 agcaggataa agccgaaagc gtatttgacg gcattcatcc aatcgcctgc cctaggcagg
     9661 atatgcccgc cgaacgtgcc gatggcaatc agcggaacgc cggtgcccaa cgccaaagtg
     9721 taaagtgcca aaccgcctaa aaccgcatcg cccgtctgac cgatgtagcc caaagcaaat
     9781 gccagcggcg gggcgacgca cggcccgaca atcagcgcgg acaatatgcc cataataaag
     9841 acggaaacga ttttaccgcc tgaaagcctg ctgctctggt tttggaaata cgactgcacg
     9901 gcgttgggaa gctggatgtt gaacagcccg aacatagaca gtgccaagac gaccattaaa
     9961 gccgatgccg ccaacaccac ccaagcctgc tgcaaccata cggtcagcag cgcgcccgtc
    10021 agtccggcaa caatgccgac cagcgtataa gtcagagcca aaccctgaac ataaacgacg
    10081 gacagcacaa acgcccgcgc cttgcccgcc tttttgtcgc cgaccacaat actggaaaca
    10141 atcggcaaca ggggatacat acaggcggta aaactcagac ccaaaccggc gagaaaaaac
    10201 gccaaaagat tggcgttgag cgtatcccaa gacagcttga aacggctgtc gccgccctca
    10261 tcccccttcg ggggcggcaa cgccccgctg ccgttttgag aggaaggctg caaaaagcgg
    10321 tctttggcgg atgccggttc gtcggtttgc ggatggtaag tgccgttgcc ggaaatatca
    10381 aactcggtat ccacgggcgg atagcacacg ccggcttcgg cacagccctg ataggtcaaa
    10441 accaatttat acggttcgcc gacagccttt gcataaggaa aggcaacctg cgcttcgtga
    10501 tggtaaaccg tctgcctgcc gaaaaactcg tcttccttct cttcgccctt gctgaaagaa
    10561 ggctgtccca acaaatccgc cggatcggtc ttgccgacga ttttcgcctg atacatatag
    10621 tatccgtcgg caatcctgaa acggacgttc acaccgtcgt cggcaacggc aagctccggc
    10681 acgaatgcct tttccggcgg cagcagatcg ttcgcatcca gcgcgaaagc tcgtccgcac
    10741 aacatcaaaa atacggcgaa caggcaaatc agttttttca taatcgaatc cgtttcagac
    10801 aaataatttg tctgcattat aaatggtaag gttgacggtg ggatttaatt tatgtaaaac
    10861 ccgccattat ccgaacctat ttccataaac atcttaccga acccgccatg tacgatgtca
    10921 atacccacga tgtccgccgc tttttcgccc gcgtgtggca gcagcggctc aatccgctgc
    10981 aactgagcgt actggaacag aaagccctcc gcattatcga agcccatccc gaataccacc
    11041 gttatctcga acgcatcgaa gaccatctgg acaccgactg gctacccgaa aacggcgaaa
    11101 gcaacccctt cctgcatatg tcgctgcatt tgtccgtcca agaacaggcg ggcatagacc
    11161 agccgcacgg catacgcgca atccacgaca ccctgtgcgc caaacgcggc tggctggaag
    11221 ccgaacacga aatgatggag gcactggcgg aaacactgtg gacggcgcaa cgctacggca
    11281 ccggtttgga tgtcaatttc tacatgaccc gactgcgcaa actcatcggc ttgggtgcag
    11341 aagatcaagc caggttgaac ccgcatgaaa tcgcctgacc acaccaaccg cctgcaaaat
    11401 gccatctgaa gcggaacaac ccctttcaga cggcatgcat ttcccccatc atttccacaa
    11461 cgcgcgtttc agcataatca accaatcctt cttatccaaa acggggcgtt gtgcaaacac
    11521 atcgtatcgg cacgcgtcca gtttctgcaa aatcaactgc gcccccaaca caatcatacg
    11581 gagttccaaa ccgatacgcc ctttcaattc gcgcgctaaa ggcgaacccg ccttcagcat
    11641 acgaaatgcg cgccggcact catacgccat cagccgctga aacgccgcat ccgccctacc
    11701 ctccgcaatc tgtccttcgg aaacaccgaa tttcaacaaa tcgtcctgcg gaatataaac
    11761 cctgcccttt tgccaatcca cagccacatc ctgccaaaaa ttcaccagtt gcaaagccgt
    11821 acagatgccg tcgctttgcg ccacgcacac cgcatccgtt ttcccgtaca aagccagcat
    11881 aatgcgtccg acagggttgg cggaacgccg gcagtaatcg gccagttcgc cgaaatgcgc
    11941 gtaccgcgtt ttaaccacgt cctgcgaaaa cgccgaaagc aaatcataaa acggctgcaa
    12001 atccaaaccg aacggcacaa ccgcctcggc atccaatcgt gcaatcaaag gatgcgccga
    12061 ccggccgccc gatgccaaca cgtccaactc gcgccgcaaa gcatccaacc ccgccaacct
    12121 ggcttcagac ggcatactgc cctcgtccgc catatcgtcc gccgtccgtg caaacgcata
    12181 caccgcatga accggcttcc tcaacctgcg cggcaaaatc agcgaaccga cgggaaaatt
    12241 ttcataatgc ccaaccgaca taccttctcc atccatcaaa caaaatgccg tctgaaacgg
    12301 aacaaaccct tttcagacgg catcagatac ctccaagctg ccggcaatca gtggtggtga
    12361 tgaccgtgcg ggccgtggac atgaccgtgt gcgatttcct catcggatgc atcgcgcacg
    12421 ctttcaaccg tagccttaaa acggattttc atgcctgcca aaggatggtt gccgtccacc
    12481 accgccttgc cgtcggcaac atcggttaca cgatagacga caacatcgcc ggtttcagga
    12541 tcgtcggctt caaacatcat gccgacttcg acttcaacag ggaacacgcc cacatcttca
    12601 atacgaacca actccgaatc ctgctcgccg aacgcatcgt cgggcgaaag cgccacatcg
    12661 accgtatcgc cggcatcctt accgtgcaac gcctcttcca ccaaagggaa aatgccgtcg
    12721 taaccgccgt gcagatacgc aatcggttct tcggttttgt ccaaaagctg attgttggca
    12781 tcatacatct cataatgcag cgaaaccacg gaattcttca cgatagccat atttgtcctt
    12841 tcaggaacag cagattgatt acaggcgcat tctaacacaa ccgccgcgcc ggccgattac
    12901 cgttaacctg ttcataaact gtacagcaca tatttcaatg taaatttttg ttattttatt
    12961 gcgatgtaac ttttttacaa cattattaaa accattccga cctgtctgcc gactttccca
    13021 atccgcctta ataaatcata caagatactg aaattatatt aatctctata atatttatcc
    13081 ctatcgaatt tttaacagca aaaccgtttt acaggattta tcaatccgcc cgccagaaaa
    13141 cttttcattc aaaccttttt cccatctgta cgacattgca atcccttatt ccatagtgca
    13201 taattacgca aattcagcga tgaatttcca acccggtttg tagtatggtc gataaagacc
    13261 tatttgtttc aataatttaa attggttcta aaggttacta aaatgaaaaa atccctgttt
    13321 gccgctgctt tgttgtcttt ggtcctggca gcctgcggcg gtgaaaaagc cgctgaagct
    13381 cccgctaccg aagcacctgc cgccgaagct cccgctgctg aagctgccgc tactgaagca
    13441 cctgccgccg aagctcccac tgctgaagct gccgctactg aagcacctgc cgccgaagct
    13501 cccgctgctg aagctgccgc taccgaagca cctgccgctg aagctcctgc tgccgaagct
    13561 gcaaaataag cattttccgc ttgcaaaaaa gcaggatacg ttcagtatcc tgcttttttg
    13621 atttttcaga cggcatcaga ttcccttcct caatcttctc cctacccttc cgacaaacat
    13681 gcctgacctt cataccgaat tttcccgact cctgccggca gatgaaattg ccgaactttc
    13741 tccgacgctt ttaaaagacc agcgcaaccg ctttacgtct gcaccagaca tcattttgca
    13801 gccgcgcagc gttgaaagcg tgcaaaccat tatgcgtttt tgttacgaac accgtattcc
    13861 ggttacgccg caaggcggca ataccggttt gtgcggcgcg gcagtatcgg aaaacggcgt
    13921 attgctgaac ctttccaaac tcaaccgcat ccgcagcatc aatttgtcag acaactgcat
    13981 aaccgtcgaa gcaggttccg tactccaaac cgtccaacag gcagccgaag cctcaaacag
    14041 gctgttccca ctcagtctcg ccagcgaagg ctcctgtcaa atcggcggca acatcgcctg
    14101 caatgccgga ggtttgaacg tattgcgtta cggcacgatg cgcgacctga ttatcggttt
    14161 ggaagtcgtc ctccccaacg gcgagctggt ttcccatctc catcccctgc ataaaaacac
    14221 caccggctac gacctgcgcc atctgtttat cggcagcgaa ggcacactgg gcatcatcac
    14281 tgccgccaca ctcaagcttt ttgcccgtcc ctcggacaag gcaacggcat gggtcggcat
    14341 acccgacatc gaatccgccg tccgcctgct gaccgaaacc caagctcact ttgccgaacg
    14401 cctatgcagt tttgagctga tcggccgttt tgccgccgag ttgtcttccg aattcagcaa
    14461 attccccctg ccgacacatt cagaatggca tattttactt gagttgaccg actcattacc
    14521 cgacagcaat cttgatgatc ggctcgtcga atttctttat aaaaaaggat ttaccgacag
    14581 cgtattggca caaagcgaac aagaacgtat ccatatgtgg gcattgcgcg aaaacatctc
    14641 cgcctcccaa cgcaaactgg gcaccagcat caaacacgat attgccgttc ctatcggacg
    14701 cgttgccgac tttgtccgcc ggtgcgccaa agatttggaa cagaatttta aaggcataca
    14761 aatcgtctgc ttcggacatc ttggcgacgg cagcctgcac tacaatactt tcctgcccga
    14821 aatcctcagc aatgaagtct atcgttacga aaacgacatc aacgacgcag tttatcgcaa
    14881 cgttcttgcc tgcaacggca cgattgccgc cgaacacggc ataggtatca tcaaaaaaca
    14941 gtggctggac aaagtacgca cgcctgccga aatcgccctg atgaaaagca tcaaacaaca
    15001 ccttgatcca tataacatta tgaatccggg caaactgctt ccgtaaccgg catttctgat
    15061 ttgcatacac aacaaagaaa gggacaatag atccgattgt cggtttagcg cgagctcgtg
    15121 agtgcggtaa aaaattggtg gaaatgacac gaaaaatgac cgcattttta aaataaaaaa
    15181 atcggcagtg aatttccctg ccgattttat tttgttacaa cttaacttaa aacgtccact
    15241 gcaaattcaa cgcaccttgt ttagcctgat gatatttgct tgtttggcgg ttgaatgagg
    15301 cttgtaaagt taagtgagat ttgattttca ctgctacacc taattggctc tcaattgccg
    15361 tcttattgtt tatcactcga cgctctccgt ctatttccac accgaaaggt ttgttgtggt
    15421 aaagcgcgtt cacagcggcg aaaggttcaa tagcgatatt tttatagagt gaaaattgag
    15481 ctttagcttg aacgccaacc cgagtttgta attggcggga gccaagtaaa ttcacgtggg
    15541 cattttcgct atcgctgaat tttccgttta cccccaaata agtcaattgt gcctgtggtt
    15601 gtaggtaaac acgaaggctg ttgccctttt tagtgaagtg ttctgccaat aaagcattgt
    15661 aacccgcttc aattgaggcg gtaatacctt ttgaagtaaa acgttctgta gcatcttcag
    15721 tgttaatgcg gtggcggaag cgttgatatt gtacccagct atccgcatac gcacctgtct
    15781 gtttgtcctg aagctgatgc caagtggcat aaatgcccgc cccaaagcct ttcacatttc
    15841 ccgttgtaag attgtctgta tctggattgc ggaaagtgct gcgttgttct gcttgtccgc
    15901 tcattaagcc aacggaaagt tgattacttt cgttttgcca tgtgaatact tcgccaccga
    15961 gttgcacacc tttacgatag ccttctacag gtgctgtttt gccttgcacc cattggttgg
    16021 aatgtccgtc aatcacacgc aaccacaagc ctttgcgtgg taaagtgcgg tcaaaaatat
    16081 cgctgttttt gtcgttcaaa cgcaaggcga ataaggtatt ggcggcttga gcctgttgtg
    16141 cataaatcga aatatcatcg cgttcttgca ctttggtaaa aaagccttct gggcgttgtt
    16201 gtaaagaaag cgtataaatt cccttttggt gtttgccaga aagacggaat gcgtgtttat
    16261 ctgctgtgcc atttactttg ataatttgat gcccgtcgag actttttaaa tcgtctattg
    16321 gattttcgaa gatgatgtcg gaagtgccag ttatattttt ttcaaaagtt aaaactggtg
    16381 tttcagagac tttatctttt agatcgtaag caaaacgaaa acgagctccg ccagcataat
    16441 cttcttttac gagtaaactt tcacttttag tattaaaacg gatgtctgca ttcgttgttt
    16501 ttaattcccc aacatcagaa tcccaacggg gttcccagag agaattttct aagcggaatt
    16561 catccaaact aattgtttgt ccgataacgt gtgagttgtc tgtaacctca atatagtgga
    16621 atggatctaa accagaatag agatgctctt gccaactagg aagcttatta agtgttgggc
    16681 gaagccattc gatataatta cctacagata aaatttcgct gttgatatgg ctatttttta
    16741 tttgtggagc taaggcgaaa atatcgctct ttattaatct tgggccagag ttttcgaagg
    16801 atggttgttc acacttatca ttattggaat tcccagtatt tacacagtcc ttaaaatatt
    16861 gctggagtgt tttttcttct tccccatttc ttttttcaac cccgaaacct cgggcgaagc
    16921 caacaaggta atcttcagtg tattccttgt cataaaaaga aatctttttt gagttgttga
    16981 tgtttttgaa ttggtgtgtt ttagagtata gtgcgggaac tttgagagag ttctgagagc
    17041 taccattcgt cggttttaga agataagttt cttgtttcca gtattcgctg cttttatctc
    17101 taagtttttt taagcgatca atttgctcat ttgttaattt agaattgtcg taaacgtaat
    17161 caacagccaa aagcggagag gtataaagaa tagaaaaaaa tagacttata ataaatgatt
    17221 tttttaaact tctgcttgct tgcttcgagt ttcataataa attttccttt gtcaagtaaa
    17281 aataaatggg gcgtggattt tagcataaaa ctgaacaaaa aatgtcattt agctcacatt
    17341 tttctctatt tatttcttat ttattaaagg taaacgtttg ctttttgcta ttttgtcaag
    17401 ccagtttgaa aatgtgtata attgccctcg ttatttacaa aaatttcagg aaaaatgacc
    17461 gcactttacc cttggctaat gccaatttat catcaaattg ctcaaacctt tgacgaaggt
    17521 ttggggcatc atgctgtgct gattaaagcg gatgctggtt taggtgtaga acgtttacac
    17581 atcaggcggc agccttgccc ataccctctg aagcactgtt tccacaatca gcgcgtatgc
    17641 ttaatcaacc gctgtttctc gcgtttccaa tccgcctctt tcatactctg acgtttgtcg
    17701 tgctgtttct taccttttgc caaaccgatt tccatcttga tttttccgcg cgaaaaatgc
    17761 aaatccagcg gcacgatggt gtagccggca cgttcggttt tgccgattaa tttgttgatt
    17821 tccgactggt tcaacaagag cttgcgcgga cgtacggcat ctggtttaat gtgtgtcgag
    17881 gctgtgggca aagccgtaat atggcagccg accagataaa acgcgtcttt tttccaatag
    17941 atataactct ctttaagctg tacgcgcgcg gcgcggattg ctttgacttc ccagccttcc
    18001 aagaccaaac cggcttcaat ccggtcttca atgaaaaaat cgtgaaatgc ttttttattg
    18061 ttcgcaatag ccataaacat cctatcaata tccgccgtca gacggcataa acccgaaaac
    18121 agaacccatc ataccgcctc ttcaaccgcc tgcacaatct tctcgggata cagcctgttg
    18181 aggcagtcgg tatgccccag cggacattcc cgcttaaaac agggcgaaca ttccaagtgc
    18241 aggctgacga ttttcgccct gtcgctcaaa ggcggcgtat gcgtcgggct ggaagaaccg
    18301 taaaccgcca ccaccttcct gcccaatgcc gccgccaagt gcatcagccc gctgtcgttg
    18361 cacacaaccg tgtccgccaa cgacagcaaa tccattgcct gcgacaaatc ggttttgccg
    18421 cacaaattga cacacatacc gtctgaaagg cagttgattt cctcggcaat ttcatcatct
    18481 ttttgcgaac cgaacagcca aacctgccaa cccgccgcca gataatgttt gcccaactcg
    18541 gcaaaatgcc ttgtcggcca acgctttgcc ggcccgaatt ccgcgcccgg gcaaaatgcc
    18601 aaaacaggct ttccaagatt caaaccgaaa gtttcgacag aaatttcccg ccgccgttca
    18661 tcaatggaaa actcggggaa tcccgaatgc ccgtcaaaat cttcctgact cggatgcgcg
    18721 agagccgtat atctgtccac catcaaaggc agacgttcct tatccagcct gcgtatatcg
    18781 ttcaacagaa aataacggct ttcaccgaca taacccgtcc ttttggcaat acccgttgct
    18841 agcgcgatga ctgccgattt caaagaaccg ggcaacacga taacctgatc gtatccgcgc
    18901 cgccccaaat ccctgccgac ccgccagcgg cgtttcaact ccaacgcacc gtgtccgaac
    18961 ggattctcaa gaatttcatt cacttccggc atacgctcga acaccgccat cgaccacttc
    19021 ggcgcgaaca catcaatcgt gcaaccgggg tgaagttcct tcaaacggcg gaacaagggc
    19081 tgggtcatca cgcagtcgcc tatccaactg ggagaaataa tcaggatttt gatggacata
    19141 acaagaaacc gaaatcagac aggcagaatt ttaccgcgaa accgttggaa aacctatctt
    19201 gtctcattcc gaacgccgta cgtgcaaata tgaaaaagcc cgaacattca agttcgggct
    19261 tcaaaattct ggctccccga cctgggctcg aaccagggac ctgcggatta acagtccgtc
    19321 gctctaccga ctgagctatc ggggaatggg gcgtattata gcgtccggaa aaaatgtgtc
    19381 aatccttaat tttggaaaaa tgggcgacaa aacgacaagc atatgaatca gaaagacatt
    19441 aagaccgatg ccttaaaagg attgccgttg tatgaatttc cacagccgtc atcacaccat
    19501 atttaagccc gatgagccgt tctgccctcc cccgcttaaa acaatgccgt ctgaacttcg
    19561 ccgcgttcca aagccagtaa aaactgtttg cggttcaaac cgcctgcgta gccggtcagt
    19621 ttgccgtcgc tgccaatgac gcggtggcag ggaatcagga cagacacctt gttctgcccg
    19681 ttggcggcgg caacggcgcg gacggctttg gggttgccca aatgctgcgc ctgttccttg
    19741 tagctgcgcg tttcaccgta aggaatcgcc aaaagcgcgt cccacgcctg tttttgaaac
    19801 gccgtaccaa tcatttccaa aggcgtggca aaggttttca gacggcattg gaaatacaaa
    19861 tccaattcct gccgcaaaag ttgcgtccgc tcatcctccc gaaacacaaa ccgtccgcgc
    19921 aaggcttttt ggacggcggc aatttcctgt tccaaatgct tctgcccgac aaattccagc
    19981 aaacacaagc ctttaccgcc gaacaccgcc agcatctcgc ccaaaggcgt ggcaatggcg
    20041 gcacacacca gctcgttcaa gctgtcggga taacgcactt ccaacagacg gatggctcgg
    20101 cggatacgga catattcttc aggcgcgcag ccgatattgt cccaaaaatc ccgatcgaac
    20161 tgcccggctt cgcattccgt cagattggga tgcggcataa cgccgcactc aaaaacccgg
    20221 gtttcgaggc aatggcggat ttcatcccat tttgacggca gattgtttaa ggaaggcagg
    20281 gtaatcattc gtttgctccg tatctctatc atggatttgg cggcaaaatc cccaattctc
    20341 gtcattcccg cacgccgatg caggaacggg ctatgacgta aatcttgagg gttgggttgc
    20401 ggcaatgcct aaatatgcga tatttctaaa gcctcaggga aaggaattaa agacttcaga
    20461 cgacctgcaa agggtcgtct gaaaccacga tttttgcatt tacgcattcc ggcacatcat
    20521 ccaaccgttt cggcacattc ctgccgccgt tgacagccta taatgaatcc acttattcat
    20581 caagcaaagg aatcatctat gcaaacccgc atcctctccg ccgtactgct ggctttttca
    20641 accgctgcct ttgccggggg cgcattcacg ctgcaattcg acaacccgtc cgaagacggc
    20701 ggcttcacgc aaaaccagct tttgagtgcg ccttacggct tcggctgttc aggcgacaat
    20761 gctccgcccg cgctgtcgtg gaaaaatccg cccgccggga caaaaagttt cgtcctgacc
    20821 gtttacgata aagacgcgcc gaccggactg ggctggatgc accgggtggt cgccgacatt
    20881 cccgccgatg tccgccgccg caatgcggcc tcgctgcaat taagccgctg cgccagcatc
    20941 gccgacgacc cgtccgcagc catatcggcg gtaatcagtt tgcggatttg ccgcatcagg
    21001 ttgacgcctt cgtacacggc aaaaccgatg ccgtcatgct gcaaccacgc caacacgccg
    21061 caaagcgcgg cctccgccgc attgtgcggc acttcttcat ccgtcagcac cgccgccgca
    21121 taatcaaacg ccgcgcccat ccgcccaggg tgcggcagct tcaccgcatc gcacactgcc
    21181 tgcgccgtcc cgtattgtgc ggcaaacctt tctacggttt cctgttcgaa agcaatccat
    21241 tgcgcctgat agaggccgtc tgaatcggga atattaataa cgtcaaacgt ctgtccgcct
    21301 gccaacgcaa ccgcctcacc cgccgcaatc ctcaattcgc gcctcgccca ccgcagcttc
    21361 ttgcttccgc gccgcacgat aagcacagct ggttcatata ccgccacgct gcggtacaag
    21421 gcggtatgat gttgcacgat gccgcccaaa gcacccaatc gttcgcgcgt atgaaagtaa
    21481 tttgacggca ctattatctc caacacgcag ggccgcacat aaggcgcagc cctgtttacc
    21541 ctgatttgga aggggttact tcccaaaaat aaaccaaaat cccactgccc cgaatggccg
    21601 agtttcaacc atattccgtc ggtgtgcctg caacttgact ccctcccctg tgggggaggg
    21661 ttggggagag ggtaaaacgg ggcagataca gacaatattt ccgttaccgc cccgatgccc
    21721 tctccctaac cctctcccac gggagaggga atggattgcc gttgaaataa atcgctctac
    21781 ataaaaaatc aatgtgttat ctcaaaccca cgttaggtct aagcaaatgg tcggacatcc
    21841 gtatccggca agcaagctgc tttcagacgg catttccagc caacaagcgc gccaatatcc
    21901 cctcatacac cgcagacagc ttcggaatat cgtccagccg cacgttttcg ttgatttggt
    21961 ggatggtggc attggacggg cctaattcga tgagttcttt tgcaatggct ttgatgaagc
    22021 gtccgtccga agtgccgccg gtggtggaca attcggcctc aatgccgcag gtttcggcaa
    22081 tggctgcgcg tgccacgtcg gtcagtttgc ccgcttgggt cagaaagggc tgccccgaac
    22141 acgaccactg caaatcgtat tgcacgccgt gtttgtccaa aatggcgtgg acgcgttgtt
    22201 tcagccctgc ttcggtggac tcggtggaga agcggaaatt gaatttgacg ttcaactcgc
    22261 ccggaatgac gttggtcgcg cctgtgccgc cgttgatatt ggaaatttga aagctggttg
    22321 gcgggaaata ttcgttgcct tcgtcccaga cttcctgcgt cagctctaac aaggccgggg
    22381 caaaagtatg cacgggattg attgccaaat gcggataggc aatatggcct tgcttgcctt
    22441 tgacggtcag gttgcccgac agcgagccgc gccggccgtt tttaatcata tcgcccaatt
    22501 tgtccacggc tgtcggttcg ccgacgatgc agtagtcaat caactcgccg cgcgctttca
    22561 acacatcgac gactttggtc gtgccgtcca gcgcgtcgcc ctcttcgtcg gaagtaatca
    22621 ggagcgcgat gctgccttga tgatcggggt gttcggcaac aaagcgttcg caggcggtaa
    22681 cgaaacaggc gatgctggtt ttcatgtccg ccgcaccgcg cccgtataat cttccgtcgc
    22741 gctcggccgg ctcgaacggg ggcgaatccc atttttcgac agggcctgtc ggtacaacgt
    22801 cggtatgccc tgcaaaacag acgacgggag ctttcgtgcc gcgtcgcaac cagatgtttt
    22861 tggtgtcgcc gaaatggagt tcttcagccg caaaaccgat tttgtgcagg cgttcggcaa
    22921 ggagtttttg gcaattttgg tcgtcggggg taacggatgg gcgggaaatc aattctttgg
    22981 cgagttctag ggattgggtt tcggtcatat ttgttcactt ttgaaattag accatctgaa
    23041 acgttctgaa tgtgattttc agacggcatt taggttaggt tggcatacgg ggtgggtatt
    23101 tttacccgtc agtcttctga atcatttgcc gtgacacgct tcgtaaagcg gcagcaaatc
    23161 ttccaccgtt tccgctatcc atttcgcgac atcctgcctg cccaaatcgt cgcgttcgat
    23221 gtgtttgccg atgcagaaga aatcttcgtc gttttgcaac cttcggtcgg attcattttg
    23281 ctgcgcgaca gtgcggtagt cgtcgtattc gctttccgcg ccgtgccaca tatcgaaaga
    23341 agcgtatttt tcagtatcaa aattatccaa ccaacggttg taatcaggca gcgcaatcgg
    23401 cgacacgtcg gctttgtagc aatgccagtc caagctgaca ctcaaacggc ggcggttgag
    23461 cagtatcgac aaaatcgcgg cggaattttt atattgttcg tatttgaagt aggcaaagaa
    23521 atgggcgcga acctgccagc cgttgcacca gcgttcgatg tgcggcggcg caaacggcgc
    23581 acccaattcg gcggcaacct gctgaatcag ctgctgccat atctgccagt tttccttgta
    23641 gtcggctttg atttgcggaa tgctttcagg ctggtatttt ttaagctggg caaactggaa
    23701 aaacgggata ttgaacaaat cgcaactttt cggggtcagc ataatatatc cttgagacga
    23761 ttgtttcaga cggcattatt tgcgccggcg cgccgccata atttcgccga tttcggtcag
    23821 tttttctttt gggataaagg tgttgcccat atcaaacagc ggctcttcaa tcgccaaatg
    23881 aacatcatat cccgccacaa aacgtttgaa cgcttcctca tcggggacat aagcgttgtc
    23941 tgcttcgagt ttggcaaatt cggcggaaac agccgcccag ttgtcgtgca gcccgatatg
    24001 ttggcgcaaa agctcgtcca cgctttcttg ggcttgcggc gcatattgca gcagcagcgg
    24061 gaagaagttt tcttcttcgt cttcatggtg cagcggcgcg gcaacgttga aatactgggc
    24121 gatttggcgg atggtttgca aaacaatctg attgcagccg ttttcggcga tatagtccga
    24181 cagcatggcg acttgtccgc aaaaacggcg cactttgccg tggcaggcat acagcatttc
    24241 aatcggttcg gcaaaggtaa cgcttttggt ttcaaacgga ttcatgtttt cgttctcaac
    24301 gggcactttt caagcagtca ttttataata aaacagcctg cacaaagcag gctgtccgtc
    24361 ttttgagact ttaagcggat taatcgacca aagtcacttt gccgttcatc aaagcaccgt
    24421 gacctgggaa ggtacaagcg aatttgtatt cgccgtcagc cagtttggca ggatccaaag
    24481 tcagggaaga ctcttcgccg ccgccgatca gtttggtgtg ggcaacaacg cgcgcatcgt
    24541 caggtttgac atagtcggta tcggcagcac ctacgccgtc tttaaatacg ccgtccatgt
    24601 cttcagcttt ggcaatcaca aggttgtgac ccatgctggc tttgggttgc gtaccggtat
    24661 gtttcagagt gatggtgaac tctttacatg ctttgctgac ttggatgtct ttggtgttga
    24721 actgcatatt gtcgttggat tcgacagttg ccgcacagtt gccggcagca ggggcttcgg
    24781 cagcatctgc aggagcagct tcggcggcag gtgcttcgga agcgggtgct tcagcagcat
    24841 gagttgcctc ggcagcaggc gcggcaggtt cttgagagca ggcagccaaa ccgataacgg
    24901 cagcagaaat cagagccaga tacgttttca taacaaatct ccaatcgata aaataatatt
    24961 cggttttacg gaaatcaaag tgcaaccgcc attaacaaaa ccttgaaaaa gattccgccg
    25021 cgttgcacaa acagatgttt cggggcggca ttttgctaca aatttcattt gaaatcaaag
    25081 cctgtctgca agtttacaat cgtttacttt aaaaggggca attttacccc gaacctattt
    25141 ctttagtatt agacccatta tcctttactt cttaatatta acggatgttt acacaaattc
    25201 ccgtatacat tttatgcgcc atgccttcta accaagtttg ccaatgcctc cgccaattcg
    25261 ggatgccgtt tttccaactt taccgccgcc gaaccgaaac tctccagcgc agccttactc
    25321 aaatgcaggg tattggtttt cggcggtttt tccggtttcg gggccagcct gaccgaaaca
    25381 gagcgtatcg aagcatcaag ccctgccaac tgcggcaata ccgacggtgc aatcattttc
    25441 aagcgcgatg ccgccatatt gtttgccgcc aaaaggacaa gcctgccgtc ttcgatacat
    25501 gccgtctgaa aatgcgggtg caggttggca ggcagcagtt ttttcacggc ggcatccaac
    25561 cgccgccact gtcccgcctg tttcaaaagt ccggaaagca gcgcgtcccg cctgcccaac
    25621 tgttccaaat tcataaaaca tacacccaaa aagattgaaa taccgcaaac gcgcctttat
    25681 ttcagacggc attagcactt tgcacaaacg cttgtgttaa aatcgcgttt tcgcccacta
    25741 ttatatcagg cgcaggaatt attcatgctg acaaacattg ccaagaaaat cttcggcagc
    25801 cgcaacgacc gcttgctgaa acaataccgt aaatccgttg ccagaatcaa cgcgctcgaa
    25861 gaacagatgc aagccctaag cgatgctgat ctgcaagcca aaactgccga attcaaacaa
    25921 cgcctcgccg acggtcagac tttggacggc attttgcccg aagccttcgc cgtctgccgc
    25981 gaagcgtccc gccgcaccct cggtatgcgc cacttcgacg tgcagcttat cggcggtatg
    26041 gtgctgcatg acggcaaaat cgccgaaatg cgtaccggcg aaggcaaaac cctggtcgcc
    26101 accctcgccg tctatctcaa cgcgctggcc ggcaaaggcg tacacgtcgt taccgtcaac
    26161 gactacctcg cctcacgcga tgcgggcatc atggaaccgc tctataattt cctcggcctt
    26221 accgtgggcg tgattatttc agatatgcag ccgttcgacc gtcaaaacgc ctatgccgcc
    26281 gatatcacct acggcaccaa taatgaattc ggcttcgact acctgcgcga caatatggtt
    26341 accgaccaat acgacaaagt gcagcgcgaa ttgaattttg ccgttgtcga tgaagtggat
    26401 tccatcttga ttgacgaagc gcgcactccg ctgattatct ccggccaggc agacgacaac
    26461 atccagttgt accaaatcat gaacaccgtc ccgccccacc tcgtccgcca agagacagaa
    26521 gaaggcgaag gcgactactg ggtagatgaa aaggcgcacc aggtcatcct gagcgaagcc
    26581 ggccacgaac acgccgagca gattctgacc caaatgggat tgctggcaga aaacgactcg
    26641 ctctattctg ccgccaacat cgccctgatg caccacctta tggcggcatt gcgcgcgcat
    26701 acgttattcc acaaagacca acattacgtt atccaagacg gcgaaatcgt catcgttgac
    26761 gaatttacag gccgtctgat gtccggccgc cgctggtccg aaggcctgca tcaagccgtg
    26821 gaagccaaag aaggcgtgga aatcaaacgc gaaaaccaaa ccctcgcctc catcaccttc
    26881 caaaactatt tccgcctgta caccaagctc tccggcatga ccggcacagc cgataccgaa
    26941 gccttcgagt tccaaagcat ctacaacctc gaaaccgtca tcatcccgac caaccgcccc
    27001 atacaacgca aagacttcaa cgaccagatt ttccgttccg ccgaagaaaa attcgaagcc
    27061 gtcgtcaaag acatcgaaga atgccacaaa cgtgggcagc ccgtcctcgt cggcacaacc
    27121 agcatcgaaa actccgaact ggtctcccat cttttgcaaa aagccggtct accgcacaac
    27181 gtcctcaacg ccaaagaaca cgaacgcgaa gccctgattg tcgcccaagc tggcaaagtc
    27241 ggcgcgatta ccgttgccac caatatggcg ggacgcggta cggacatcgt tttaggcggc
    27301 aacctgaagc accaaaccga tgccatccgt gccgacgaaa ccttgagcga cgaagagaaa
    27361 caggcacaaa tcgccgcact cgaaaacggc tggcaggcag aacacgataa agtaatggaa
    27421 gcaggcggcc tgcacatcat cggtacggaa cgccacgaaa gccgccgtat cgacaaccaa
    27481 ttgcgcggac gttccggccg tcagggcgac cccggatcca gccgtttcta cctttcgttt
    27541 gaagacccgc tgctgcgctt attcgcactc gaccgcgccg ccgccatcct caaccgtctc
    27601 gcccccgaac gcggcgttgc catcgaacac aacctgctga cgcgccaaat cgaaggggcg
    27661 caacgcaaag tcgaaggcag aaacttcgat atgcgcaaac aggttttgga atacgacgac
    27721 gttgccaacg aacagcgcaa agtcatttac agccagcgca acgaaattct gaccagcaaa
    27781 gacatcagcg acctgatgca ggaaatccgt tctgatgtcg tcagcgacct cgtggatacc
    27841 tatatgccgc ccgacagcat ggaagaacaa tgggacatcc cgactttgga gaaccgtctg
    27901 gctgccgaat tcagactgca cgaagacatc caatcctggc tgaaggcgga caatgcgatt
    27961 gacggtcaag acatcaaaga acgcctgatc gaacgcatcg aaaacgaata tgccgccaaa
    28021 accgaactgg tcggcaagca ggcaatggcc gatttcgagc gcaacgtgat gttgcaggtc
    28081 atcgacaacc aatggcgcga acacctcgcc gctatggact acctgcgaca aggcatacac
    28141 ctgcgcagct atgcccaaaa aaatccgaag caggaatata aacgtgaagc ctttaccatg
    28201 ttccaagacc tgtggaacgg catcaaattc catattgcct ccctgcttac ctcggttcaa
    28261 atcgaacaaa accctgtcgc ggtggttgaa gagcaaccca tcggcaacat ccagtccatc
    28321 cattccgaat cgcccgatat ggaagaactt ttgggtcagt cgcaaaccga tctggttacc
    28381 gaagccttta atcccgatgg gacggatttc agccccgaag ccttggaagc gcggggacaa
    28441 atcgtccacc gcaacgaccc ctgcccctgc ggcagcggtt tgaaatacaa acaatgccac
    28501 ggcaaactgg cttgagcgtt tgaacgcaaa tgccgtctga acatccggtt tccggcttca
    28561 gacggcattt tgcctgaacc gccacatccg accgccattc cgaaaaatcc cgatttcgta
    28621 ccgtccaaac taaaaacagg catccgcccc gccgaactat gattccatcc gacttcattg
    28681 acgaacttct ggccaaaacc gatattgtcg acatcatcga cgaacaggtt ccgctgaaaa
    28741 aaggcggggc gaactatatg gcgtgttgcc cgttccacaa ggaaaaaacg ccgtcgtttt
    28801 cggtcagtcc gaccaagcag ttttaccact gtttcagttg cggggcgcac ggttcggcga
    28861 ttggttttgt gatggaacat caggggctgt cgtttccgga ggcagtgcag ttccttgccg
    28921 accgcgtggg tatgattgtg cctaaagtgc gcgggcagaa cgataatccc gaagtccgtg
    28981 ccgaacgtaa gaaaaagcag cagacgctgg aagaaacgac agcggcggcg gctgattttt
    29041 atgcgcaaca gttgaaattc aatccgacgg cgaaggctta tttggacaag cgcggcttga
    29101 gcgcggaagt catcgcgcat tatggtttgg gctacgcgcc cgacggctgg cagcctttgg
    29161 cgcaagtatt ccaaccgtac ccgaataccg cgttggtgga tacgggtatg gtgattgaca
    29221 atgaggggcg gtattacgac cgattccgtc atcggattat gttccccatc cgcaatccgc
    29281 gcgggcaggt catcggtttc ggcggcaggg tactggacga ctccaaaccc aaatatctaa
    29341 actctcccga tacgcctttg tttgataagg ggaaaaacct ttacggcttg tatgaggggc
    29401 gtgccgctgt caaggaagcg gagcggattc tggtggtcga aggctatatg gacgtggtcg
    29461 cgctggcaca gttcggcgtg ggctacggtg tggcggcctt gggcacggca acaacagctg
    29521 aacacgtcaa aatcctaatg cgtcaggcgg acagtattta tttctgtttc gacggcgaca
    29581 gcgcggggcg caaagcggct tggcgcgcgc tggaaaacgc gctgccgcag ttgaaggacg
    29641 acaaatcgct gcattttttg ttcctgccgg aagaacacga ccccgacagt tacatccgtg
    29701 cctacggcaa agcgcaattt gaagacgcgc ttctgaatca aagcaagccc ttgtcggagt
    29761 atttctggga acacctttca gacggcattc atctcaatac gcaggaaggc aaggcggaat
    29821 tggtaaaaac cagttcgccg cttttggcgc agattaccgc gccggcattg gcttatttgt
    29881 taaaacaacg gcttagcgaa ctggtcggca tcgaccccga caacctcgcg caactgctgg
    29941 gacaggaagc gccgaagcgg cacgtcaaac aaaaaaacta caaactgccg ccgatttccg
    30001 tcaaacagcc cgttatgccg acattggtgc aacggcaaat ccgcagcctc ttgataaatc
    30061 cggattgggc tgcatatata gacctgcccg attatctggc gttggacggc gatttcgcct
    30121 gccttgccaa cctcgccgaa accattaaaa accatccttc cgtgccggca accgcacagg
    30181 ttttggaaca tatgcgcggt tcgccctacg aagaaacaat caaccgcatc ttccggtcgg
    30241 cccttcaatc ggaagaaatg gaaggcggcg gcgaagaaga ttgcgaaaac ttccaaatcg
    30301 gcatcaaaaa actgctcaat gagttaaaat acagccaaat cgaaacatta aaacaaaaaa
    30361 gcctgcaatc cggcttaaat gaaagcgaga aaaaactttt gctgtcgctg ctgaccgcaa
    30421 aacaaaattg accggcggat tccgccatcc gtaaaccgtt atgccgtctg aaaagcattc
    30481 accccggctg caacaacgac acctgcagaa cacccatccc caaaagcctt cagacggcat
    30541 cagagtaccc tactctgcca cgccttcagg tgcgtccaaa cgcaaaccgt cggcatcttg
    30601 ccaacagaaa gcagacaatg tccaaaaacc aaaatcacga agaataccaa gacgatgccc
    30661 gccccctgac gattgaggaa caacgcgcgc gcctgcgcca actcatcatt atgggcaaag
    30721 aacgcggcta catcacctac tccgaaatca atgacgcact gcccgacgat atgtccgatg
    30781 ccgatcaaat cgacaacatc gtcagtatga tttccggttt gggcatccaa gttaccgaac
    30841 acgcccccga tgcggaagac atattgttaa gcgacaatgc cgccgttacc gacgatgatg
    30901 ccgtcgaaga agccgaggcc gccctttcca gcgcagattc cgagttcggc aggacgaccg
    30961 accccgtccg tatgtatatg cgcgaaatgg ggcaggtcga cctgctgacc cgcgaagacg
    31021 aaatcatcat cgccaaaaaa atcgaaaacg ccctgaaaaa tatggttcag gccatctccg
    31081 cctgcccggg ttccattgcg gaaatcttag aactcatcga aaaaatccgc aaagacgaaa
    31141 tccgcgtcga cgaagtcgta gaagccatta tcgacccgaa tgaagtattg ctcaacgaat
    31201 tgggcttggg gcacttggaa accacagcac ccgagaaacc ttccaacgac aattcggacg
    31261 aaaacgaaga cgacgaagaa tcggaagaag atgcggatga aatctcggca gccaatctcg
    31321 ccgaattgaa acaaaaagtc atcggccact ttgcccaaat cgaaaaagac tacaaaaaaa
    31381 tgattggccg tttggaaaaa caccacagcc ggcacaaaga ctatctcgcc taccgcgacg
    31441 cgattgccaa caaactgctg gaagtccgtt tcgccacccg gcaaatcgac agcctcagca
    31501 gcagcctgcg cggaaaagta gaaaacatcc gcaaactcga acgcgaaatc cgcgacatct
    31561 gcctcgaccg cgtccatatg gagcgcgact acttcatcca aaacttcctg cccgaaatca
    31621 ccaatctgga atggattgaa gaagaaatcg ccaaaggcag ggtttggagc gacgcgctcg
    31681 accgcttccg ccacgccatc ctcgaaaaac aaaccgagtt ggcggatatg gaaaaagaaa
    31741 cccgcatttc catcgaagag ttgaaagaaa tcaacaaaaa tatggtgtcg agcgaaaaag
    31801 aaaccgcagc cgccaaacag gaaatgattc aggcaaactt gcgcctcgtg atttccatcg
    31861 ccaaaaaata caccaaccgg ggcttacaat tccttgatct gattcaggaa ggcaacatcg
    31921 gtttgatgaa agcggtcgat aagttcgaat accgcagagg ctataaattc tccacctacg
    31981 caacctggtg gatccgccag gcaattacac gctcgattgc cgatcaggcg cgtaccatcc
    32041 gcattccggt acatatgatt gaaaccatca acaagatgaa ccgcatctcg cgccaacacc
    32101 ttcaagaaac cggcgaagaa cccgattccg ccaaacttgc cgaactgatg cagatgcccg
    32161 aagacaaaat ccgcaaaatc atgaaaatcg ccaaagagcc gatttcgatg gaaaccccca
    32221 tcggcgacga cgacgattcg cacttgggcg acttcatcga agatgccaac aatgttgcgc
    32281 cggccgatgc ggcaatgtac accagcctgc acgaagtaac caaagaaatc ctcgaaagcc
    32341 tgaccccgcg tgaggcaaaa gtcctgcgta tgcgtttcgg catcgatatg aacaccgacc
    32401 acacgctgga agaagtcggc agacagtttg acgtaacgcg cgaacgcatc cgacaaatcg
    32461 aggcaaaagc actccgcaag ctgcggcatc cgacaagaag cgaccgtttg agaagtttct
    32521 tggacagcga agacagcaag ctgtaaacca aaaaaccgca ggtttcaaat acctgcggtt
    32581 ttttcttaca caataaacaa cgcttccaca tatcccacac tcctatcccg agacctttgc
    32641 aaaattcccc aaaatcccct aaattcccac caagccattt aggggatttt ccatgagcac
    32701 cttcttccaa caaaccgccc aagccatgat tgccaaacac atcgaccgtt tcccgctatt
    32761 gaagttggac caagtgattg attggcagcc gatcgaacaa tacctgaacc gtcaaagaat
    32821 ccgttacgtc cgagaccacc gcggtcgtcc cgcctatccc ctgttgtcca tgttcaaagc
    32881 cgtcctgctc ggacaatggc acagtctctc cgatcccgaa ctcgaacaca gcctcatcac
    32941 ccgcatcgat ttcaacctgt tttgccgttt tgacgaactg agcatccctg attacagcac
    33001 cttatgccgc taccgcaatt ggctggcgca agacgacacc ctgtccgaat tgctcaaact
    33061 gatcaaccg
//
LOCUS       DAXHTR010000005        27538 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.005, whole genome shotgun sequence.
ACCESSION   DAXHTR010000005 DAXHTR010000000
VERSION     DAXHTR010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27538
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.005"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(109..948)
                     /locus_tag="ACFNML_000166"
     CDS             complement(109..948)
                     /locus_tag="ACFNML_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HFC6391627.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            1233..1308
                     /locus_tag="ACFNML_000167"
     tRNA            1233..1308
                     /locus_tag="ACFNML_000167"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1266..1268,aa:Arg,seq:ccg)
     gene            complement(1373..2566)
                     /locus_tag="ACFNML_000168"
     CDS             complement(1373..2566)
                     /locus_tag="ACFNML_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFC6391628.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAAAPAPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIATVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFEGAGFEVGTYPYYDPATVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEAELVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFAYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(2587..3675)
                     /gene="trmA"
                     /locus_tag="ACFNML_000169"
     CDS             complement(2587..3675)
                     /gene="trmA"
                     /locus_tag="ACFNML_000169"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFC6391629.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFDAASEAVNCLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKKLGDAWQQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTRKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEQTLKLVSQFDNIIYISCNPETLRANLDTLVETH
                     AVERAALFDQFPFTPHIESSVLLKKKIL"
     gene            3818..4918
                     /gene="aroC"
                     /locus_tag="ACFNML_000170"
     CDS             3818..4918
                     /gene="aroC"
                     /locus_tag="ACFNML_000170"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFC6391630.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRREADQVEILSGVFEGKTTGTPIALLIRNTDQRSKDYGNIATSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFGSVVQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDIHVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVQVNTPDITLSNK"
     gene            5010..5414
                     /locus_tag="ACFNML_000171"
     CDS             5010..5414
                     /locus_tag="ACFNML_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFC6391631.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDSALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAEAAGSSAAE"
     gene            complement(5488..7473)
                     /gene="parE"
                     /locus_tag="ACFNML_000172"
     CDS             complement(5488..7473)
                     /gene="parE"
                     /locus_tag="ACFNML_000172"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFC6391632.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPLFSCENYISDEHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDSILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(7540..8064)
                     /locus_tag="ACFNML_000173"
     CDS             complement(7540..8064)
                     /locus_tag="ACFNML_000173"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFC6391633.1"
                     /translation="MLDREGYRPNVGIILTNNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQPSGNR"
     gene            8230..9525
                     /gene="serS"
                     /locus_tag="ACFNML_000174"
     CDS             8230..9525
                     /gene="serS"
                     /locus_tag="ACFNML_000174"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFC6391634.1"
                     /translation="MLDIQLLRSNTAAVAERLARRGYDFDTARFDALEERRKSVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLDAVQKELDAWL
                     LSIPNLPHESVPAGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(9603..10601)
                     /locus_tag="ACFNML_000175"
     CDS             complement(9603..10601)
                     /locus_tag="ACFNML_000175"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6391635.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQIGDCGNAVRADG"
     gene            complement(10726..11802)
                     /gene="prfA"
                     /locus_tag="ACFNML_000176"
     CDS             complement(10726..11802)
                     /gene="prfA"
                     /locus_tag="ACFNML_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFC6391636.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKIGALDTELQKLLLP
                     KDADDDKNIFIEVRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIIGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(11906..12676)
                     /locus_tag="ACFNML_000177"
     CDS             complement(11906..12676)
                     /locus_tag="ACFNML_000177"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6391637.1"
                     /translation="MNFTDTHCHLADPALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDFVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPPQRERQIQVFVRQLAIAQTLRRRVIIHNLKATADIAAAVKQTGFAQGGIVHA
                     FSGSAEEARVLTKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKKEI
                     NTPANIPGIAKIAAEIRGTCVEEIAKVTERNADALLGR"
     gene            complement(12684..13676)
                     /locus_tag="ACFNML_000178"
     CDS             complement(12684..13676)
                     /locus_tag="ACFNML_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFC6391638.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWSYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TASGFDNPHFGVIAEWDETQGWYNLRIPSQDAAAVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVQDFTQQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSEGWGAEDVHKELVRLKLV"
     gene            complement(13743..14426)
                     /locus_tag="ACFNML_000179"
     CDS             complement(13743..14426)
                     /locus_tag="ACFNML_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6391639.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLVGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(14615..15949)
                     /gene="glmM"
                     /locus_tag="ACFNML_000180"
     CDS             complement(14615..15949)
                     /gene="glmM"
                     /locus_tag="ACFNML_000180"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFC6391640.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVVQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPSHSDLRGLKLVIDTANGAGYGVAPKVFHELGAQVVSIGDEPNGYNINEK
                     CGATYTKTLQAAVLQHEANYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMENALKEQGVDFCRAKVGDRYVLEQLNQRGWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(16079..16933)
                     /gene="folP"
                     /locus_tag="ACFNML_000181"
     CDS             complement(16079..16933)
                     /gene="folP"
                     /locus_tag="ACFNML_000181"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFC6391641.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNARTA
                     LAHAEQLLKEGADILDIGGESMRPGAADYVSPEEEWARVAPVLAEVAGWGVPISLDTR
                     RTVIMEKALALGVVDIINDVAALTDKGAVELLARQADTGICLMHMQGLPENMQINPKY
                     QDVVGEVVRYLKERAAECIAAGIAPQRITLDPGFGFGKNLQHNIALMRHLPELMAETG
                     FPLLIGVSRKSMIGELTGEADAAARVHGSVAAALASVARGAQIVRVHDVKATADALKA
                     WEALGINL"
     gene            complement(17073..17966)
                     /locus_tag="ACFNML_000182"
     CDS             complement(17073..17966)
                     /locus_tag="ACFNML_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HFC6391642.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWRNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQTASAHGAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGSLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLSHTKDHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            18057..20171
                     /locus_tag="ACFNML_000183"
     CDS             18057..20171
                     /locus_tag="ACFNML_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFC6391643.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAIGLHASVYRILTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSDGIGTPKISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTIGGEYARWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(20236..21714)
                     /gene="ubiD"
                     /locus_tag="ACFNML_000184"
     CDS             complement(20236..21714)
                     /gene="ubiD"
                     /locus_tag="ACFNML_000184"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFC6391644.1"
                     /translation="MKYKDLRDFIVMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     SALLFENPIKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMAPNVVKNAPCQEIVWEGENVDLYKLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFRKLNPDTSYP
                     VSVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDYFPVFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVDNTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWGELGL"
     gene            complement(21734..22249)
                     /locus_tag="ACFNML_000185"
     CDS             complement(21734..22249)
                     /locus_tag="ACFNML_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HFC6391645.1"
                     /translation="MYTCTSCGEKHEDMPAIGFTAPDPYNQLSEEERIAYQAECDSDF
                     CIIRYPDQTDRFIRAVLPIPIIGHEETLEYGVWVSVSEKSFNDYQSHFHDNPENAVYF
                     GMICNRLPSYESDTFGLHCNVVTQPDGQRPLLQLHQSGGHPLVRDFYHGIEYAEAQAR
                     IGAVSESDCSG"
     gene            complement(22256..22690)
                     /locus_tag="ACFNML_000186"
     CDS             complement(22256..22690)
                     /locus_tag="ACFNML_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391646.1"
                     /translation="MCGLWLLRALTAKLPQQRYAALFITLFFAAVLVFGRHDSMYWYP
                     VLVNLMMLAVFGGSLFAGQTVIERLARLQQPDLPEKAVRYTRRVTQVWCVFFIANGTL
                     AALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYRKLVLKV"
     gene            complement(22971..23054)
                     /locus_tag="ACFNML_000187"
                     /pseudo
     CDS             complement(22971..23054)
                     /locus_tag="ACFNML_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
     gene            complement(23057..23557)
                     /locus_tag="ACFNML_000188"
                     /pseudo
     CDS             complement(23057..23557)
                     /locus_tag="ACFNML_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714696.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            23604..24168
                     /locus_tag="ACFNML_000189"
                     /pseudo
     CDS             23604..24168
                     /locus_tag="ACFNML_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            24172..25281
                     /locus_tag="ACFNML_000190"
     CDS             24172..25281
                     /locus_tag="ACFNML_000190"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFC6391647.1"
                     /translation="MEAQLGAPIEDYAPPKHWTRKQLRSMGRVSYLCVDAAEQALTDA
                     GLLGDESITDGRMGVACGSSSGSTKDIGDVGELLLTGTSRNFSANTYVRMMPHTTAAN
                     IGIFFGLKGRIIPTSSACSSGSQGIGYAYEAIKYGLTDMMLAGGGEEFFSSEVYVFDS
                     LYAASRRNGEPEKTPRPYDANRDGLVIGEGAGIFVLEELEHAKRRGAIIYAELVGYGA
                     NSDAYHISTPRPDAQGAILAFQTALQHAGLAPEDIGWINLHGTGTHHNDSMESRAVAA
                     VFGNNTPCTSTKPQTGHTLGAAGAIEAAFAWGIADRQSNPEGKLPPRLWDGQNDPDLP
                     AINLTGSGSRWETEKHIAASSSFAFGGSNCVLIIG"
     gene            25335..25410
                     /locus_tag="ACFNML_000191"
     tRNA            25335..25410
                     /locus_tag="ACFNML_000191"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25368..25370,aa:Ile,seq:cat)
     gene            25485..26243
                     /locus_tag="ACFNML_000192"
     CDS             25485..26243
                     /locus_tag="ACFNML_000192"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6391648.1"
                     /translation="MKKVSVLIVAKNEANHIRECIESCRFDKEVIVIDDHSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAEKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            26244..27308
                     /locus_tag="ACFNML_000193"
     CDS             26244..27308
                     /locus_tag="ACFNML_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6391649.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRGCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLCALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKAKYRIVH
                     NGIDTGRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIIEHHQSGILLDRLTPESLADEIERLVLNPETKNALATAGH
                     QCVANRFTINHTADKLLDAI"
BASE COUNT         6402 a         7756 c         7110 g         6270 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aattcactat aaattcccat aaaaaaacgg
       61 agcagatacc tgccccgttt ttatttaatc cgaaatttta atctaaattt agaattttgc
      121 accggattgg tttgccatat agtcaacagc cgctttgact tcgtcatcgc tcaaacctgc
      181 attgccgcct ttggcaggca tcgcgttaaa gccttcaagg gcgtgtttgt gcaaggtttc
      241 tttgcctttt ttgatacgcg gtgcccaatc gtcttttttg cctatgccgg gaataccggg
      301 aatcgaaccg ccgtggcaca cctgacaggt tgcttcgaag acttttttac cgtcaacgcc
      361 gaccgcaggg gctgccgcac ccttgtcttc tgccttcgct tctgccggag ctgcactatc
      421 ggcaggagca gaagctgttc ctgaagcggc attgtcggca ggcgcagcct catcaggatt
      481 cgggaaagaa ccgccgcttt tgttcgccat gtaagtaatc gcccgtttaa gttcctgatc
      541 ggtcaggtct gccgcaccgc cttttgcagg catggcgtta aagccgttca gcgcgtgttg
      601 gaacaaggta tcgaagcctt gcgcgatacg cggtgcccaa tcgccgttgt gttccagttt
      661 cggagcgttc ggcacattgc tgtccgccgc gtggcattgg atacagattt tgccgaaaat
      721 ctgttcgcct tggcgttcgc cgacggggat gccgtcgccc atcgtcaatt gtccgacagg
      781 ctggatacgg gtctgcgttg ctgcttccgt agtggcatcg acatcgccga acgagccgct
      841 gcccgccagc ttaatcagga aataaaggac tgcaataaca ataacgatac cgctcacaag
      901 ggtaaacagt gcagagcctt gggctttgtt gtcgcggagt tgtttcattt ggtaggcctc
      961 gccgtcaggt taggttgtgc tgtaaattat agtttggtgt gttaaacgca gttaacaata
     1021 ttttgctgga ttatactgaa ttcacagggt ctttccaatc gctatcattg aaaatatgaa
     1081 aaaatttgcc aacggtatct gtataaaaca aacaatcctt tgaaaataat ggtttatcct
     1141 caagaaaact ctccttatgc cgccatacgc cgcctgccgg cgcaagataa cctttgccaa
     1201 tttgcagaat ttacgttaac cttacgtttt acgcacccat agctcagttg gaagagtgtc
     1261 agtttccgaa gctggaggtc acaggttcga tccctgttgg gtgcgccaat tataaagaga
     1321 ccgtctgaaa gataaatatt tttcagacgg tcttttgact tacttcaaac tcttatttca
     1381 agacttccgc aaatgcgcgg gcaacatagt cggtattcga cgtattcagt ccggcgacgc
     1441 acatcctgcc ggaatccagc aggtaaacgg caaattcgtc gcgcagcctg cggacttgtt
     1501 ccacgctcaa tcctgtgtag ccgaacatgc cgcgctgttt gatgaaataa gcgaaatcgc
     1561 gatcggggat ttgcgcagtt aagacatcat aaagtttctg ccgcatcgca cggatgcggt
     1621 cgcgcatcat ataaacctcg ttttgccaca aggcgtaaag ttcggggctg ttcatcacgt
     1681 cggcggcgat atacgcgccg tgcgcgggcg ggctggagta gatacggcgg acagtgaatt
     1741 tgagctgtcc gaataccaat tccgcttctt ccttattcgg gcaaaccacg ctcaggccgc
     1801 cgacgcgctc gccgtagagc gacaggtttt tcgagaagga attgctgacg aacagcggca
     1861 attccatttc caccgctttg cggacggcgt aggcatcgct gtccaaatcg ccgccgaatc
     1921 cttggtaggc aatgtccata aacggaatca gtttgcgcgt tttgatgatg tgcaacactt
     1981 cgtcccattg ttgttccgac atatccacgc cggtcgggtt gtggcagcag ggatggagga
     2041 tcaggacgct gttttcgggc agggtgttga aaaacgcggt catttcgtcg aatttcacgc
     2101 cgacagtggc agggtcgtaa tatgggtaag tgccaacctc gaaacctgca ccttcaaaaa
     2161 tgccgcgatg gttgtcccaa gtcgggtcgc tgacgtaggc gcgcgcttca ggaaaccagc
     2221 ggtgcaggaa atctgctccg actttgagcg cgcccgagcc gcccaaggtt tgcacggtag
     2281 cgatgcgtcc ttgcgcgagc gcagggttgt ctttgccgaa caataaatgc tgcaccgcgc
     2341 tgcggtaagt gtccaagcct tccatcggca ggtagggcga cggcgcaggc gcggcggcgc
     2401 gggcggtttc ggctcggctc acggattcca acacgggcat tttgccttcg tcgtcgaaat
     2461 aaatgcctat gctcaaattg actttttcag ggcgcgggtc gtttttgaag gtttcgacca
     2521 aactcaaaat cggatcgccg ggatagtatt cgatgtgtcg gtacatagtt cttacctctt
     2581 gatttttcaa agtattttct ttttcaacag tacgctgctt tcaatatgcg gcgtaaacgg
     2641 aaattggtcg aacagggcgg cacgttcgac cgcatgggtt tccaccaagg tgtccaaatt
     2701 agcacgcagc gtttcgggat tgcaggaaat gtagatgatg ttgtcaaact gcgacaccag
     2761 cttcaaagtt tgctcatcga tgccggcacg cggcggatcg acgaaaatag tggaaaatgc
     2821 gtaatccgtc aaagcaatac cgccatcctt aaggcgttta aactcacgtt ttctggtata
     2881 ggcttcggta aattcttcag cagacagacg ggcgatttta atgttgccga tgcggttggc
     2941 ttcgatattc cattgcgccg cgctgacgga ggttttggag atttcggttg ccaaaacacg
     3001 ttcaaacttt tcagataaag gaagcgtaaa gttgccgttt ccgcaataca gttcgagcaa
     3061 atccccatcc aatccttctg ctgcaccgca ggcccattca agcattttct gacacacggc
     3121 ggcattcggt tgggtaaaac tgccttcaat ttgccgataa cggaaatccc ggttgccgac
     3181 cttcaaagtt tccgttacat agtcctgttt taagactatt ttctgtcccc tgctccgccc
     3241 aataacggaa atatccaact gttgctgtaa cgcttgcgcc gcctgttgcc atgcgtcgcc
     3301 gagttttttg tggtaaatca tggtaaccag catttccccg ctgagcgtgg acagaaattc
     3361 gacggcatac caacggtttc tcagttcggc agattgggcg gcaacggcaa tcaattcagg
     3421 catcaggcaa ttgactgcct ctgaagcagc atcgaagcgg tcgcaacgga tcaggcttgc
     3481 cccgccagcc ttctgccctt tttcaaacat ggcgtaaaac atttcaccgc cttcgtgcca
     3541 aatacggaac tcggcacgca tacggtaatg tttgtccgga gattcataca cttcccactc
     3601 aggaacatcc aaacctgcaa aaagggtttt aaggtggtct tttttatctt gaagctgctg
     3661 cgtgtagtcg ctcatatcgc tatcctgaaa aatcagaccg gattataagc cgattcactg
     3721 caaaacacgc aaccccttta ccggcatata acccgttttc ctccaacttt tatgctaaaa
     3781 tccgccgtct ccaacatacc gccgacagaa ggaaaatatg gcaggcaaca ctttcggaca
     3841 actcttcacc gtgaccacgt tcggcgaaag ccacggcgca ggtttgggct gcatcatcga
     3901 cggctgcccg cccggcttgg aattaagcga agcggatatc caatttgacc tcgaccggcg
     3961 caaacccggc accagccgcc acgttaccca acgccgcgaa gccgaccaag tcgaaatcct
     4021 ctccggcgta ttcgaaggca aaaccaccgg cacacccatc gccctcttaa tccgcaatac
     4081 cgaccagcgc agcaaagact atggcaacat cgccaccagc ttccgccccg gccacgccga
     4141 ctatacctat tggcacaaat acggcacgcg cgactaccgg ggcggcggca ggagttccgc
     4201 ccgcgaaacc gccgcccgcg ttgctgccgg agccgttgcc aaaaaatggt tgaaagaaaa
     4261 attcggcacg gaaatcaccg cctacgtgac ccaagtcggc gaaaaagaaa tccggtttga
     4321 aggctgcgaa cacatttccc aaaatccttt ttttgccgcc aaccatagcc aaattgccga
     4381 gctggaaaac tatatggaca gcgtgcgcaa atccttggat tccgtcggcg cgaagctgca
     4441 tatcgaagcc gccaacgtcc ctgtcggctt gggcgaacct gtttttgacc gcctcgatgc
     4501 cgaaatcgcc tacgcgatga tgggcatcaa cgccgtcaaa ggcgtggaaa tcggcgcagg
     4561 ttttggcagc gttgtccaac gcggcagcga acacggcgac gaactgaccc cgcaaggctt
     4621 cctgtccaac cactcaggcg gcatcctcgg cggcatcagc accggacaag acatccacgt
     4681 caatatcgcc atcaaaccca ccagctccat cgccaccccg cgccgcagta tcgacatcaa
     4741 cggcaacccc atcgaactcg ccacgcacgg caggcacgac ccctgcgtcg gcttgcgcgc
     4801 cgcgccgatt gcagaagcta tgctggcatt ggttttaatc gaccacgccc tgcgccatcg
     4861 cgcgcaaaat gcggatgttc aggttaatac gcccgacatt accctttcaa acaaataaaa
     4921 atttagccaa aacacagact ttataataga ataccgagca ttgccgtgcc gctttgacgc
     4981 acgggtttgt ttagaggaat acaaccgaaa tgacacaaga aaccgctttg ggcgcggcac
     5041 tgaaatccgc cgtccaaact atgagcaaaa agaaacagac cgaaatgatc gccgaccaca
     5101 tctacggcaa atacgatgta ttcaaacgct tcaaaccgtt ggcgctcggc attgatcagg
     5161 atttgattgc cgctttgccg caatacgatt ccgcactgat tgcacgcgtc ctcgccaacc
     5221 actgccgccg tccgcgttat ctgaaagcct tggcgcgcgg cggcaaacgt ttcgatttga
     5281 acaaccgttt caaaggcgaa gttacccccg aagaacaggc gatcgcgcaa aaccatcctt
     5341 tcgtgcagca ggctttgcaa cagcagtccg cccaagctgc cgccgaagca gccggatctt
     5401 ccgcagcaga ataaatcccc aaacgaaatg ccgtctgaaa accgatttgg tttcagacgg
     5461 cattttttcg tatgcggcaa tcacggttca aatatccaat tccgccgtat cgccttcgcg
     5521 ttccatccaa gcgcggcggg cggcggcttc gcctttgccc atcagtttga cgaagatgtc
     5581 gcgcgtctcg tcatctgcac cttctgggat ttgtacctgc aacaggcggc gggtgtcagg
     5641 gtgcatggtg gtgtctttga gctggtcggg gttcatttcg cccaagccct tgaaacggct
     5701 gatggaatag gcggtttctt tgacgccttc tttttgcagc cgctccaaaa tgctgtcgag
     5761 ttcgttttgg tcgagggcgt agaatttgcg ggcaggtttg cttttgcctt gtgcgttgac
     5821 atcgacgcgg aacagcggcg gctgggcgac gtagatgtgt ccgtcggcaa ccagtttcgg
     5881 gaagtggcgg tagaataagg tcagcagcaa aacttgaata tgcgagccgt ccacgtcggc
     5941 atcggacagg atggcgattt tgccgtagcg caggccgctt aaatcgggat ggtcgttaat
     6001 accgtgcgga tcgacgccga tggcgacgga aatgtcgtgg atttcggcgt tgccgaagag
     6061 ttggtcgggg tggacttcaa agctgttgag cactttgccg cgcaggggca ggatggcttg
     6121 ggtggctttg tcgcgggcga gtttggctga gccgccggcg gaatcgcctt cgacgaggaa
     6181 gagttcgttt tcgcggatgt cttcgctttc gcagtcggtc agcttaccgg gcaggacggc
     6241 gacgccgctg ccttttttct tttcaatctt tttaaccgaa cgcatccgcg cctgtgcctg
     6301 acggatggcg agttcggcga tttttttgcc gaagtccacg ttttggttca gccacagttc
     6361 caaagggtcg cccgatacgg tagcaacgag tttcagcgcg tcgcggttgg tcagtttgtc
     6421 tttggtctga ccttggaact gcgggtcgag gacacgggca gagaggacaa aggcagtttt
     6481 gccgaacacg tcgtcgcttt gcactttcac gccgcgcggt aagaggttgt gcaggttgat
     6541 gaagttgctg acggcgttga acacggcttg tttcaagccc gcttcgtgcg tgccgcccag
     6601 cggggtgggg atgaggttga catagctttc gttggcacac gagccttctt ccagccaagt
     6661 cagggcaaag gcggcgcctt cgccgatgct gaaatcgccg ttgtgttcgt ctgaaatgta
     6721 gttttcgcaa gagaacagcg gtacggcttc ctgcgcgtcg gcaatcaggt cggtcagata
     6781 gcttttcagg ccgtcggggt aatgccaggt ttgggtgtgc gcttcgtctt cgcctttgac
     6841 cggacgggtc agggaaacgc gcacacccgg cagcagcacg gctttggcac gcagcaggcg
     6901 ttcgagttcg ggaatgctgt aattcgggct ttcaaaatat tttccgtccg gccacacgcg
     6961 cacttcggtg ccgctgtctt tgacggcgca tttgcccact tgtgccaacg gttcgaccac
     7021 gtcgccgccg gcaaacacga tacggtggat tttgccttcg cgtttgaccg ttacctcaag
     7081 gcgggtggaa agggcgttgg tcacggatac gcccacgccg tgcaggccgc ctgaaaaggc
     7141 atacgcgctg ccgccgtctt ttttgttgaa cttgccgcct gcgtgcagac gggtgaacac
     7201 gagttcgact acgggtacgc cttcttcagg gtgcagaccg acgggaatac cgcggccgtt
     7261 gtcgcgtacg gaaagcgaac cgtcttcatg aatttgcacg tcgattgccg tagcgaaacc
     7321 gcccaacgcc tcgtccgccg cgttatcaat cacttcctga cagatatggg tcgggctgtc
     7381 ggtgcgggtg tacataccgg gacgttcttt gaccggttcc aaacctttga ggacggtgat
     7441 gctggattcg ctgtattggt tgtttttagc cataggaata atctgaaagt aagaaaaaca
     7501 acgctttcag acggcctgaa agcgttgcgt tccgttgttt taccggttgc cggaaggttg
     7561 gcgggcggca aagtcttcat aactttccat accgcgcagg aagcgggaag agagttcttt
     7621 caacgccccc aaataaacgt cgcgtttgaa atcaatcact tcgtcaacgg gcgcccaata
     7681 ttgatgccaa cgccagccgt caaattcggg gtggcgggtg gcgcgcaggt tgacatcgca
     7741 atctcggccg gtcaggcgca ggagatacca aatctgcttc tgcccgcgat aggatccgcg
     7801 ccattcgcgg cgtacccagt tgttcggcac gtcataacgc agccagtcgc gcgtgcggcc
     7861 gataattttg acgtgttgcg gcaaaagccc gacttcttcg tacaactcgc ggtacatggc
     7921 ggtttcgggg ctttcgcccg gcttgatgcc gccttgcgga aactgccaag aatgttcgcg
     7981 cacgcgctta ccccaaaaga cttcgtcgcg gttgttggtt aagatgatac cgacattggg
     8041 gcgatagcct tctctgtcca acacggtgtc gccctccgtt aaattcaatc ttgggatttt
     8101 cccacaaatc gaacggtttt gacaaatcag acggtatggt tcaagcatgt cgaaacacgg
     8161 acggattcgg gaaaatatct taaatttggt ttacaataat gtgtttcaaa ttaattcggg
     8221 aatcagacca tgttagatat ccaattgctc cgcagcaaca ccgccgccgt tgccgaacgg
     8281 cttgcacggc gcggttatga ctttgatacc gcacgttttg acgcactgga agaacgacgc
     8341 aagtccgttc aggtgaaaac tgaagaacta caagcctcgc gcaacagcat ttccaaacaa
     8401 atcggcgcac tgaaaggtca gggcaaacac gaagaagcgc aggcggcgat ggatcaagtc
     8461 gcccaaatca aaaccgattt ggaacaggct gccgccgatt tggatgccgt tcaaaaagaa
     8521 ttggacgcat ggttgttgag catacctaac ctgccgcacg aaagcgtacc tgccggtaaa
     8581 gacgaaaccg aaaacgtcga agtccgcaaa gtcggcaccc cgcgcgaatt tgactttgaa
     8641 atcaaagacc atgtcgattt gggcgaacct ttgggtttgg atttcgaagg tggcgcgaaa
     8701 ctttccggcg cacgatttac cgtgatgcgc ggacaaatcg cccgcctgca ccgtgcgctg
     8761 gcgcaattca tgctcgatac gcacacgctg aaacacggct acaccgagca ttacacaccc
     8821 tatatcgtgg acgacaccac cttgcaaggc acgggtcagc tgccgaagtt tgcagaagat
     8881 ttgttccacg ttacccgtgg cggcgacgag agcaaaacca cgcaatatct gattccgaca
     8941 gctgaagtta ccctgaccaa taccgttgcc ggcagcatta tcccgtccga acaactgccg
     9001 ctgaagctga ccgcgcattc gccctgtttc cgcagcgagg cgggttcgta cggcaaagac
     9061 acgcgcggtc tgattcgcca gcaccagttc gacaaagtgg aaatggttca aatcgttcat
     9121 cccgaaaaat catacgaaac gctggaagaa atggtcggcc atgccgaaaa catcctgaag
     9181 gctttggaac tgccctaccg cgtgattacc ctgtgtaccg gcgacatggg cttcggcgca
     9241 accaaaacgt atgatttgga agtttgggtg ccggcgcaaa atacctaccg cgaaatctca
     9301 agctgctcca actgcgaaga tttccaagcc cgccgcatga aggcgcgttt caaagacgaa
     9361 aacggcaaaa accgcttggt acatactttg aacggctccg gcttggctgt cggcagaacg
     9421 ctggtcgccg tattggaaaa ccatcaaaac gccgacggca gcatcaacat ccctgccgca
     9481 ctgcaaccgt atatgggcgg cgttgccaag ttggaagtga aataagtttg caagctgcct
     9541 gaacgtcaaa tgccgtctga aacctggttc agacagcatt tcctttaaac ttttaaaaca
     9601 cgtcagccgt cggcacgaac cgcattgccg caatcgccga tctgtccgac ctcgcggata
     9661 ttggacagcg taacttccga aatattaccc aacgcctctt ccgtcaaaaa cgcctgatga
     9721 ccggtaaaca gcacgttatg gcaggacgac aaccggcgga acacgtcgtc ggtaatcaca
     9781 tcgttggatt tgtcttcaaa aaacagctcg cgctcgttct cgtacacatc catgcccaat
     9841 gcgccgattt tccggcgttt caacgcctca atcgcggcgg cactgtcaat cagcccgccc
     9901 cggctggtgt tgataatcat cacgccgtct ttcattttgt cgaacgccgc ttcgttcagc
     9961 atatagtggt tttccggcgt ggcggggcaa tgcagcgtga tgatgtccga ctgggcatac
    10021 agctcgtcca aatccacata cctgccgccg attttttccg cttcggggtt gcaaaacgga
    10081 tcgtgagcca gcaggttcat gccgaaaccc tttaaaatcc gcatggttgc aataccgatt
    10141 ttccccgtgc cgataacgcc cgccgttttg ccgtacatat tgaaaccggt cagaccttcc
    10201 agcgaaaaat tcgcatcgcg ggtacgctga taggctttgt ggatacggcg gttcaaagtc
    10261 agcatcagac cgaccgtatg ttccgcaacc gattcgggcg aataggcagg cacgcgcacg
    10321 actttcaagc ccaactcttc agccgccttt aaatccacat tattgaagcc ggcacaacgc
    10381 aacgccacag ttttcacgcc aatttgcgcc aatttttcca acacgggccg gctgccgtcg
    10441 tcgtttacaa aaatacaaac cgcttccgcg ccttccgcca ttttcgccgt tttcgcatcc
    10501 agcataaaat caaaaaactc cagctcgaag ccgaaatgcc ggttggcgcg ggtaaaatgt
    10561 tcgcggtcat agcttttcgt accgtaaatc gcaatcttca tcaatatgtc cagttgtcgt
    10621 ctatggttga gaaacggcat aatacactga attcaaacaa atcagtagaa tatggtggat
    10681 taaaattgat tgcatgcacg gcatttccat ttcaaaacac aaaactcaat cgcccattgc
    10741 cgccaaaagc tccgcctgat gttcggcaat cagggcatcg gtgatttctg ccaaatcgcc
    10801 gtccatcaca aaatccagct tgtgcagggt gaggttgatg cggtggtctg ttaccctgcc
    10861 ttggggatag ttgtaggtgc ggatgcgttc gctgcggtcg ccgctgccga tgagggattt
    10921 gcgttcggcg gcttctttgg cttgggcttc gcgtttttgc gcgtcgttca ggcgggcggc
    10981 gagaactttc attgcctgcg ctttgttggc gtgttggctg cggccgtctt ggcattcgac
    11041 caccatgccg gtgggcaggt gggtaatgcg gacggcggaa tcggttttgt tgatgtgctg
    11101 accgcctgcg ccggatgcgc ggaaggtgtc gatgcgaagg tcgacggggt tcaactcgat
    11161 gtcttccagt tcgtccgctt cgggcatgac ggcaacggtg caggcggagg tgtggatgcg
    11221 gccttggctt tcggtggcgg ggacgcgctg cacgcggtgt ccgcccgatt caaatttcaa
    11281 acggctgtac gcgcctaagc cgataatacg ggcgataact tctttatagc cgcccaactc
    11341 gctttcgttg gcggacacga tttcaacctg ccagcggttg cgttcggcgt agcggctgta
    11401 catacgcagc aaatcgccag caaacagcgc ggcttcgtcg ccgcccgttc cggcgcgtac
    11461 ttcgataaag atgtttttgt cgtcgtcggc atctttgggc agcagcagtt tttgcaattc
    11521 ggtatcgagt gcgccgattt tggctttcgc cgcttcgatt tcttcggcgg caaagtcttt
    11581 catttcgggg tcggacaaca tttcttcggc atccgccaaa tcgctttgcg ccagttgata
    11641 gttttggaat acttcgacga cgggggtcag ttcagcgtgt tcgcgcgtga gcttgcggta
    11701 gttgtccata tcggatgtgg cttcgggttg ccctaaaagg tgggtaactt cttccaaccg
    11761 gtcgccgagt tgttgcagtt tttctaagat agacggcttc ataattcttc cataacaaac
    11821 gccgcctgaa tttcagacgg catcaacact gaattattat aataggtttt ccggacattc
    11881 aaaaagataa tctcagatgg ataacctacc gtcccaacag ggcatcggca ttgcgctccg
    11941 ttaccttggc aatctcttcc acacaggttc cgcggatttc cgcagcaatc tttgcaatac
    12001 ccggaatatt ggcaggcgta ttaatctctt ttttcagcat aaacgggctg tcggtttcca
    12061 atacgaaatc cccgtcgttc aaggctttaa gcgtatcgcg cactttacgc gcgttcggat
    12121 tgagcaacaa cgaaccgatg ccgattttga aacccagttt cgtcaacacg cgcgcttctt
    12181 ccgcgctgcc tgagaaggcg tgaacgatgc cgccttgggc aaagcctgtc tgtttgacgg
    12241 cggcggcgat gtcggcggtg gctttgagat tatggataat cacgcggcgg cgcagggttt
    12301 gcgcgatggc aagctggcgg acgaaaactt ggatttgccg ttcgcgctgc ggcggcgttt
    12361 gggttttgtc gtaaaaatcc aaaccgattt cgccgaccca tgcctgcgga taacgtgcca
    12421 acatcgcttc caggcggacg aaatcacgct cggcaatgcc gtctgaaaac caaggatgga
    12481 tgccgagcgc aatacggatt tgaccgtgtt cggacggcat ttccgacagt tccgccacgc
    12541 tttgccaatc ctgcggacag gtggcgggaa cgataaaccg cttcacccca actttccgcg
    12601 cggcggtcaa aatgtgcggc aggttttcgc gtaaggcggg atcggcgagg tggcagtggg
    12661 tgtcggtgaa gttcattccg gtttcacact aactttagtc ttactaattc tttgtgaaca
    12721 tcttccgcgc cccatccttc ggaaacggca agcgtcatca atgcggttgc tgtttccaaa
    12781 ttgcatttgc cgccgttgat gatgcctgag ttgcggaacg cgttgccttg cgcgtaaacg
    12841 gcagcggttc tgccttgtcg gacttggctg atgttgagca gcagtttgcc ttgctgcgta
    12901 aagtcttgca cggcgcggat gaaaccttcg tctgcgggcg tgttgccgtg tccgtaactt
    12961 tgcaggataa gggcgtgggc gggaagctgt ccgagtccgt cggcaagttc ttggacggca
    13021 aagccgggaa taagcgtgcg gacggcgatt tttgcctgcg ggtcaggata gcggattttg
    13081 aggccgtctg aaacggccgc cgcatcttgg gacggaatac gaagattgta ccagccttga
    13141 gtttcatccc attcggcgat gacaccgaaa tgcggattat cgaagcctga ggcggtttcg
    13201 gtgctgactt tgctgctgcc gacggcggga taaagtttgc cgtcaaaggc aataagcgtc
    13261 tgcttcaatc cgagtgggaa tgcggcaacg gcagtcgcca gattgcgcgg cgcgtcgctg
    13321 cccacggcgt tgtacgacca ttgggaaccg gtcaggacga tcggcttgtc caaaccctgc
    13381 agcgcgaggg cgaacagatt ggcggtatag gcgagcgtgt ccgtaccgtg cagcaccaaa
    13441 acgccgtcgt actgcggcag cgcggcggca atgatgtcca gccaagcgca ccagttttgc
    13501 agttggacgg cggaagaatc aatcagcgtc ccacaaacgt gccaatcgaa atccatgccg
    13561 tttgaaaacg gcgcgagtgc ctgcccgacc agcgcggtat cggggcgcag gccttcgctg
    13621 ctttgcgtca tgccgatggt gccgccggta taaaggacaa aaattttctg tttcatggct
    13681 catcctgaaa atgcaaagac cgcctgaaac gcgaaatgcg tccggacggt cgaaaatctg
    13741 atttactgct gcttctgtgc cgcttttttc gctgcctttt ccgccttgcg tttggcgcgt
    13801 ttttcgctca attgtgcgcg gtaaagctga taatgtcggc gtttgcgcca ccagaaccac
    13861 gccagcgccg ccgccagcac gcccaatgcg atgaagatgc cggattgcag gctgtgcatt
    13921 ttcgccatca gccaatcgat gttgtgcgcg ccgtactcgc ccaagtaaat ccaaacgggc
    13981 acggaaatca gcgcggcaag cccgtccata atcagaaagc gcagatacga tactttgcgg
    14041 ctgatgccgg cggtaacgaa aacggcagtc cgcaaacccg gcaggaagcg ggcgacaaac
    14101 agaacccagt tgccgtattt gtcgaatttt tcctgaacct gtgcgtaacg tttcggcgtc
    14161 atgatgcgcg caatcggttt gaacttgagg attttctgcc cccaaattcg tccggcggcg
    14221 aacatgatgc cgtccccgac caatacgccg agcataccga ctgcaaacat aatatgcgga
    14281 ttggtataac ccatacccga aatcacgccg cctgttacca aggtcaaatc ctcggggatc
    14341 ggcacgccga aaccgcagat gaccaaaacg aaaaacacgg ccgcatagcc gtattcgaca
    14401 aaaaaggctt ccaaaagggc aaacatagcg gatattccat tgtcggagat aaaaagtcag
    14461 aacaagccga aacattttct gcgcgaaacg gcattctatc aaagattatg ccgtctgaaa
    14521 gcggcaaggg gcagattcgc gccctctgcc ggacgttcag acggcattta aacaggaaaa
    14581 ccgccgctgt tgaaacggtt ttgcctgatt ttgcctaaag gctgccgatg gcggaagcaa
    14641 tacgttccgc accgtcgcgc gcccaatccg cctgccgcgc ctccaccatc acacgcacga
    14701 cgggttcggt tcccgaagcg cgcaacacga cacgcccttt gccttcgagt tctttttcca
    14761 cttccgccaa cacgtctttt gaggcttcct gccagttttg ccctttgccg atacggacat
    14821 taatcatcgt ttgcggatac ggctgccaat cggcgcaaac ggtggcgagg tcttggttca
    14881 gcgtttgcag tgccgccaaa acttgcagcg cggaaataat gccgtcgccg gtgttgtgtt
    14941 tgtccatgca caaaatatgg ccgctggctt cgccgccgat gagccagccg cgttggttca
    15001 actgttccaa cacatagcgg tcgccaactt tggcgcggca gaaatcgacg ccctgctctt
    15061 tcagggcgtt ttccatcgcc atattggtca tgaccgtgcc gaccacgccg ccgatgttga
    15121 taccttctcg ggcgcgggct ttagcgatga cgtaaatcag gctgtcgccg tcgtaaacct
    15181 taccgttttt atcgaccatc atcaagcggt cgccgtcgcc gtctaaggcg atgccgtagt
    15241 tggcttcatg ctgtaaaacg gcggcctgga gtgtcttggt ataagtcgca ccgcattttt
    15301 cattgatatt gtagccgttg ggttcgtcgc cgatgctgac gacttgtgcg cccagttcgt
    15361 gaaacacctt gggagcgaca ccgtaccccg cgccgttggc ggtatcgata accaacttca
    15421 aaccccgaag gtcggaatgg ctgggaaagg tggatttgca aaattcgata tagcggtcgt
    15481 ccgcaccgct gatgcggcgg gcgcgcccga ggcgtgcaga aggctgggtt ttcatttctt
    15541 cgtcgatttt ggcttcgatt tccaactcga tttcatcgga aagcttcacg ccgccttcgg
    15601 cgaagaattt gatgccgttg tcggaatagg cgttgtgcga cgcggaaatc atcacgccgg
    15661 cggacaggcg caacgcgcgg gtcagataag ccacgccggg cgtgggcagc ggtccggtct
    15721 gtaccacatt cacacccgcc gccgtaaaac cggccaccaa agcggcttcc agcatatagc
    15781 cggaaatgcg cgtgtctttg ccgatgagga cggtcggttt ttggtcggtg tcgtgctgca
    15841 ccaaaacctg ccccgccgca tagccgagtt tcaatacaaa atcgggcgta atcggaaact
    15901 gcccgacttc gccgcgcacg ccgtccgtgc cgaaatattt ttttgccatg tgttgctccg
    15961 agaatgtgaa ccgttgtccg agattataca gtctgtttgt gccctgctgt ctgcaccgtt
    16021 gatgccgtct gaagccgccc cgtccttttc agacggcatg aagtatgcga accgctgttt
    16081 acagattgat gcccaacgct tcccacgcct tcaacgcatc cgccgtcgcc ttcacatcat
    16141 gcacccgcac gatttgcgcg ccacgcgcta cggaagccag cgccgccgcc acgctgccgt
    16201 gtacgcgcgc cgctgcgtcc gcctcgccgg tcagctcgcc tatcatgctt ttgcgcgaca
    16261 caccgatcag cagcgggaaa cccgtttctg ccatcaattc gggcaagtgc cgcatcagcg
    16321 cgatattgtg ttgcaggttt ttgccgaaac cgaaacccgg atcaagcgtg atgcgttgcg
    16381 gagcgatgcc tgccgcgata cattccgccg ccctctcttt caaataccgc accacttcgc
    16441 cgaccacatc ttgatatttg ggattaatct gcatgttttc gggcaaaccc tgcatgtgca
    16501 tcaggcaaat gcccgtgtcc gcctgacgcg ccagcaattc gaccgcgccc ttgtcagtca
    16561 acgccgccac atcattaata atatcgacga caccgagtgc caacgctttt tccataatca
    16621 ccgtacggcg cgtgtccaaa ctgatgggaa caccccaccc tgctacttcc gccaacacag
    16681 gcgcaacccg cgcccattct tcttccggcg aaacataatc cgccgcaccc ggccgcatcg
    16741 attcgccacc gatgtcgaga atgtctgcac cttcttttaa aagctgttcg gcgtgtgcca
    16801 aggctgttcg ggcgttttgc gaatacgcgc cgccgtcgga aaaagaatcg ggcgtgagat
    16861 tcacgatgcc catgattttc ggcttgtcca aaccgatttc aaaccgtcct gcctgccaaa
    16921 cgtgtcgtgc catctgaact cctcccaaaa taaaaaacag attatatgcc gtctgaaacc
    16981 gtcttgtgcg cttcagacgg caccgctatt cgggcggcag acggcatgtt gtccgaatgt
    17041 ctgctccgcc tttgaatctg ccggtatgcc tgctatccgc ccgacttttc aaaacaggtt
    17101 ccgacgattc cgcacgcgcc tgccgccttt gccaagccgt acaggatttc ctgcggcata
    17161 tcgcggttcc ataatcccgt aatattcgca atcacgggca gatggctgat ttggcggact
    17221 ttcacgatgg attcgacatc caaacggtag ggatggtctt tggtatggct caacacgccc
    17281 gactcgccca gaatcaggtg gcggttgccg cgcgagacga catattctgc ggcattcaac
    17341 caatcttcgg cagaatggtg cttgtcttta cacagcacca agggaatgtt caggcttccg
    17401 gcttcattca atacggcaag atcggacatc agcccgccgc ccaaatacag gatgtccgcc
    17461 cccgcattca aagccgcttc gacatggcgg acgttgcgga cgcgcaccaa tacgggtttc
    17521 cctgcgccat gcgccgatgc ggtctgttcc gccaaccgtc tgcaccgtcc ccgcccttca
    17581 tccgcacttg aagtgtcgta taagtttgcc gaagtgaaaa acggatccag aaacactgca
    17641 tccgcattgc gccatactga cggttctgcg gcgatacgga cggtttcccc gccgccgaaa
    17701 gccacgcctt tggcggcaac gcggctgtct tccgcccgat tttcccgact gacggttttc
    17761 catgtatcca aaatgcggac ggctttctcg acctccggca gcgtctgcac ctccctgacg
    17821 ctcaaaaccc tatcgtcgcc gattgcgccg atgacagtac gctcgtcgcc gtgagaaatg
    17881 tgttctcgca gacctctgct gcggataaag gcgacaacgc cggcaatgtc cgcttcggcg
    17941 gcacgcctgc tcatgacaat aatcatattt cctcctgaca caagaaacgg cctgcccaaa
    18001 ataggatttt tgcaagccgt gttatactgt ggcgtgtttt acagattgtt cgggctatgg
    18061 atttattatc ggttttccac aaataccgtc tgaaatatgc ggtggccgtg ctgacgatac
    18121 tgcttttggc ggcaatcggg ctgcacgctt ccgtatatcg catcctcaca cctgaaaaca
    18181 tcagaagccg cctccaacaa agcattgctc atacgcaccg gaaaatctcg tttgatgcgg
    18241 acattcagcg caggctcctg ccccggccga ccgtcatcct gaaaaacctg accattaccg
    18301 aacccggcgg cgaccggact gccgtttccg tccaagaaac caaaatcgga ttgagctgga
    18361 aaaacctgtg gtcggatcag atacagattg aaaaatgggt ggtttcgagt gcggaacttg
    18421 ccctgacgcg cgacgggaaa ggtgtttgga acatccaaga cctgatcgac agccaaaaac
    18481 gccaagcctc agtcaaccgc attatcgtcg aaaacagcac cgtccgcctc aatttcctgc
    18541 aggaacagct tatcctgaag gaaatcaacc tcaacctgca atcccccgat tcgtcggggc
    18601 agccgtttga aagttcgggc atactggttt ggggaaagct gtccgtcccg tggaaaagca
    18661 gggggctgtt cctttcagac ggcatcggca cgcccaaaat ctcaccgttc cattttgaag
    18721 cttccacttc gctggacgga cacggcatca ccatttccac caccggcagc ccttctgtcc
    18781 gtttcaacgc cggcggagcg gatgccgccg gcctcggcct gcgtgcagac acttccttcc
    18841 gcaacctcca cctgaccgcc caaatcccta cgctggcact caggaacaac agcattaaaa
    18901 ttgaaaccgt caacggcgca tttaccatcg gcggcgaata tgcccgatgg gacggttcgt
    18961 tcaaactcga caaagccaac ctgcactccg gcatcgccaa catcggcaac gccgaaatct
    19021 ccggcagctt caaaacaccg cgccaccaga ccaacttctc cctcaattcg ccgctcgtat
    19081 ggacggaaaa caaagggctg gacgcgccgc gcctgtatgt atcgaccctt caggataccg
    19141 tcaaccgcct gccgcaaccc cgtttcatca gccggctcga cggttcgctg tccgtaccga
    19201 atctgcaaaa ttggaatgcc gaattaaacg gcacattcga ccgccaaacc gttgccgcga
    19261 aattcagata cacacatgaa gacgcaccgc atctggaagc cgccgtcgca ctgcaaaaat
    19321 tgaacctgac cccctatctt gacgacgtgc ggcaacaaaa cggcaaaata tttcccgaca
    19381 ccctcgccaa gctgtccggc gacatcgagg cgcacctgaa aatcggaaaa gtccaacttc
    19441 ccggcctgca actggacgat atggaaacct acctccacgc cgacaaagac catatcgcgc
    19501 tcagccgttt caagtcaggg ctttacggcg gccataccga aggcggcatc agcatcgcca
    19561 acacccgtcc cgccacttac cgcctgcaac agaatgcaag caacatccaa atccaaccgc
    19621 tgctgcaaga cctgttcggc ttccacagct tcagcggcaa cggcgacgcg gtcatcgacc
    19681 tgaccgcggg cggcgaaacc cgaaaagagc ttatccgctc gcttcagggc agcctgtcgc
    19741 taaatatttc caacggtgca tggcacggta tcgacatgga caatatcctg aaaaacggca
    19801 tttcgggcaa aactgccgac aatgccgcac ccagcacacc cttccaccga ttcacgctca
    19861 acagcgaaat ttcagacggc atcagccgcc acatcgatac cgaactcttc tccgacagcc
    19921 tctatgttac cagcaacggc tataccaatc tggatacgca ggaattgtct gaagatgtcc
    19981 ttatccgcaa cgccgtccat ccgaaaaaca aaccgattcc cctgaaaatc accggtacgg
    20041 tggacaagcc gtccattacc gtcgattacg gcaggctgac cggcggcatc aattcgcgca
    20101 aagagaaaca gaaaatcctc gaagacaccc tgctggaaca atggcagtgg ctcaaaccta
    20161 aagaaccgta aacatcctgc gtacaaaaat gccgtctgaa acgccctcgc gcttcagacg
    20221 gcattgccgt aaaacctaca agcccaattc cccccaaatc ccatcaatct tcgccgtaac
    20281 cgcagggtct tttttgatga cgcgccccca ttcgcggtcg gtttctcccg gccatttgtt
    20341 ggtcgcatcc aaacccattt tgccgccgag tccgctgacg gggctggcga agtcgaggta
    20401 gtcgatgggc gtgttgtcca ccaaaacggt gtcgcgcacg gggtccatgc gcgtggttac
    20461 cgcccagatg acttctttcc agtcgcgcac gttcacatcg tcatccacca cgatgatgaa
    20521 tttggtatac ataaactggc gcaggaacga ccagcagccc atcatcacgc gcttggcgtg
    20581 tccggcgtac tgttttttca tgctcaccac cgccatgcgg taggagcagc cttcgggcgg
    20641 caggtagaaa tcggtgattt cggggaactg cttttgcaaa agcggtacga acacttcgtt
    20701 caacgccacg cccaaaacgg caggctcatc aggcggtttg cccgtgtagg tcgaatggta
    20761 aatcgggttt tcgcgcatgg taatgcgttc gaccgtaaac acagggaaat aatcctgctc
    20821 gttgtaatag cccgtgtggt cgccgtatgg gccttccaac gcggtttcgt tcggatgaat
    20881 gacgccttcc agtacgattt cggcacgtgc gggcacttgc aaatcgttgc cgatacattt
    20941 caccagttcc gtccgcgaac cgcgcagcag tccggcaaac tggtattcgc tcaaggtatc
    21001 gggaacaggc gttaccgcgc ccaaaatggt ggcggggtcg cagccgagca caacggagac
    21061 gggatacgac gtatcaggat tgagtttgcg gaattcctga taatccaacg cgccgccgcg
    21121 atgcgacagc cagcgcataa tcagcttgtt tttgccgatg agttgttggc ggtaaatgcc
    21181 gagattttgg cgttttttgt gcggcccgcg cgttacggtc aagccccatg ttaccagcgg
    21241 cgcaacgtct tccggccagc aatgctgaat cggaagttta tacaaatcaa cgttttcgcc
    21301 ttcccacacg atttcctgac acggcgcgtt tttcaccacg ttcggcgcca tgctccaaat
    21361 gtctttcagt agcggcagtt tggaaaacgc atctttgatg cctttgggcg gttcgggttc
    21421 tttcaaatac gccagcgtct gcccgatttc acgcagcttg gacacgctgt ccgcgcccat
    21481 gcccatcgcc acacgttcgg gcgtgccgaa caggtttgcc aacacgggat aatcgtagcg
    21541 cgtaccgtcg ggcttaatcg ggttttcaaa caacaacgcc gacccttcgg cacgcagcac
    21601 gcggtcggcg atttcggtca tttccaaatg cggggaaacg gggtgggcga cgcgcttgag
    21661 tttgccttgc tgctcgagca tgacgatgaa gtcgcgcagg tctttgtatt tcatatttat
    21721 cctttttgtc cttttatcct gaacaatccg attcggatac cgcccctatc cttgcctgtg
    21781 cttcggcata ttctatgccg tgataaaagt cgcgtaccag cggatgtccg ccgctttgat
    21841 gaagttgcag caaagggcgt tgaccatcgg gttgggtaac gacattgcaa tgcaaaccga
    21901 aggtgtcgga ttcataagag ggcagccggt tgcagatcat gccgaaataa acggcgtttt
    21961 ccggattgtc gtgaaagtgg ctttgatagt cgttaaaact cttttcgctg acggacaccc
    22021 acacgccata ttccagcgtt tcctcatgcc cgataatcgg aatcggcagc accgcgcgga
    22081 taaagcggtc ggtttggtcg ggatagcgga tgatgcagaa atcagaatcg cattctgctt
    22141 gataagcaat gcgttcttct tcactgagtt gattataggg atcgggggcg gtaaagccga
    22201 ttgcgggcat atcttcatgt ttttcgccgc aggaagtgca agtgtacata aggtttcaga
    22261 ctttcaaaac gagtttgcgg taaagccatt cgccggcaaa cagcatcccc atcaatacat
    22321 aggcaatcac gcctgtataa accgcccacc aatcatatcg ccccaaccgg gccaacaaag
    22381 cggcaagcgt cccgttggca ataaagaata cgcaccaaac ctgcgttacc cggcgggtat
    22441 agcgcacagc tttttcaggc aggtcgggct gttgcagccg cgcgagcctt tctatgaccg
    22501 tctgtccggc aaacaggctg ccgccgaaca ccgccaacat catcagattg acgaggacgg
    22561 gataccaata catcgaatca tgccgcccga acaccaatac tgcggcaaaa aacagtgtaa
    22621 taaacaaagc cgcataacgc tgttggggca gtttggcagt cagggcgcgc agcagccaca
    22681 acccgcacat cgccgccgcc agccagacaa accagcccgc ctccctgccg taatatagtg
    22741 gattaacaaa aaccagtaca gcgttgcctc gccttgccgt actgtctgcg gcttcgtcgc
    22801 cttgtcctga tttttgttaa tccactatac cacaaagcgg gataggcaat gcttaatacg
    22861 gtcagaaaaa tatgtccgaa aaaaccgggt ttcattttga atccgcacaa atgttttcag
    22921 acggcatccg ataaaaacat gccgtctgaa aaataattag aaatacccga ttagcccgcc
    22981 tgaatcttca ataccgcctg taccacgtcg ttgacggtgc ggacattgcg gaaatcttca
    23041 gcctgcagct tgcgggctgc cacagccatt cacgattgcg atatacccgg cggcattccc
    23101 gactgccgtt cagcagaaag cgcacccatt ccaaaccgct ccaatatgcc tcgggcagca
    23161 taccggcttc agacggcata tcgtccgaag cagataaagt caggctgtaa cgcgtccctt
    23221 tggtcagaac catcgccaaa gcataagcaa acggcgcgcg cgttgccgat acggcatatc
    23281 cttccggcag cggatcgtcc gccgccaaaa ccaaaaccga cccgcatccc tcttccaaca
    23341 gtgatgccgc ttccgccaat gctgtttcca caccgtcggc acacacggcc aatgccgtct
    23401 gctcgctcat atcccgcctc aatatcgacc attaccccgc cgttgcattg tgcaccgaca
    23461 aaccgaacga agtcggcgac acgatttgcg atttcaacag ttccaaccac aaatcgaaac
    23521 tgcgcgccat ttccccgtcg tgcgaggcat aaactatagt ggattaaatt ttactagatt
    23581 agcagatatg ttaccctcga aatatgaaga taacacactg caaattaaag aaaaaagtac
    23641 agaaagaact gctccatttt tttgtactgg aagttaccgc ccgttctgcc gccgatattt
    23701 tgggtatcca tcccaattcg gcagcactgt tctaccgtaa aatccgcacg gttatcaacc
    23761 atcatttggt cttggctgcc gatgaggttt ttgaggatcc tgtcgagccg gacgaaagcg
    23821 atttcggcgg acggcgtaaa ggcagacgtg gtcgcggtgc ggcaggaaaa gtggttgttt
    23881 tcggcattct gaaacgcaac ggacgggtct ataccgttgt cgtaaacaat gccaagtctg
    23941 aaacgttact ccctgtcatc aaaaagaaaa tcatgccgga cagtattgtt tataccgata
    24001 gtctgagcag ctgcgacaag ttggacgtga gcagttttat ccattaccgc atcaaccatt
    24061 ccaaggaatt tgcagaccgt caaaaccaca ttaacggcat tgagaacttt tggaatcagg
    24121 caaaacgcgt cttgcgaaaa tatagtggat tggcacgaac gtttccccga attggaagcg
    24181 caactgggtg cgccgattga ggattacgcg ccgccgaaac attggacgcg caagcagctc
    24241 agaagtatgg ggcgtgtgtc gtatttgtgc gtcgatgcgg cggagcaggc tttgacggat
    24301 gccggtttgc tcggggacga aagcattacc gacggacgga tgggcgttgc ctgcggctct
    24361 tccagcggca gcaccaaaga catcggcgat gtgggcgaat tgttgctgac cggcacgtcg
    24421 cgcaacttca gtgccaacac ctatgtgcgt atgatgccgc acaccaccgc cgccaatatc
    24481 ggcatctttt tcgggctgaa aggacgcatc atcccgacat cgagcgcgtg ttcgtccggc
    24541 agccaaggca taggttatgc ctacgaagcc atcaaatacg gtctgaccga tatgatgctg
    24601 gcgggcggag gcgaagaatt tttctcgtcc gaagtgtatg ttttcgactc gctttatgcc
    24661 gccagccgcc gcaacggcga accggaaaaa accccgcgcc catacgacgc gaaccgcgac
    24721 gggctggtca tcggcgaagg cgcggggatt ttcgtgctgg aagaattgga acacgccaaa
    24781 cggcgcggtg cgataattta cgccgaactc gtcggctacg gagccaacag cgatgcctac
    24841 catatttcca cgccccgccc cgacgcgcaa ggcgcaatcc ttgcctttca gacggcattg
    24901 caacacgcag gtcttgcgcc cgaagacatc ggctggatta atctgcacgg caccgggacg
    24961 caccacaacg acagtatgga aagccgtgcc gttgcagcgg ttttcggcaa caatacgccc
    25021 tgcacgtcca ccaagccgca aaccggacac acgctgggcg cggcgggcgc aatcgaagcc
    25081 gcgttcgcgt ggggcattgc cgaccggcaa agcaatcccg aagggaaact tccgccccgg
    25141 ctttgggacg ggcagaacga ccccgacctg cccgccatca acctgaccgg cagcggcagc
    25201 cgctgggaaa ccgaaaaaca cattgccgcc agctcgtcgt ttgccttcgg aggaagcaac
    25261 tgcgtcttaa tcatcggatg aaataagttt gtcaatccca ccgctatgct atacaatacg
    25321 cgcctactct tgacgggtct gtagctcagg ggttagagca ggggactcat aatcccttgg
    25381 tcgtgggttc gaaccccacc ggacccacca attcccaagc ccggacgtat gtttgggctt
    25441 ttttgccgcc ctgtgaaacc aaaatgcttt gagaaacctt gataatgaaa aaagtcagcg
    25501 tattgattgt tgccaaaaac gaagcaaacc acattcggga atgtattgaa agttgccgtt
    25561 tcgataaaga agttatcgtt attgacgacc acagcaccga caatactgcc gaaattgccg
    25621 agggtttggg tgcaaaagtc ttcagacggc atttgaacgg ggatttcgga gcgcaaaaaa
    25681 catttgccat cgaacaggca ggcggagaat gggttttcct gattgatgca gacgaacgct
    25741 gcacgccgga actatctgat gaaatctcaa aaattgtcca aaccggcgat tatgccgcct
    25801 attttgtcga acgccgcaac cttttcccca accatcccgc cacacacggc gcgatgcgtc
    25861 ccgacagcgt atgccgtctg atgccgaaaa aagacagttc ggtgcaaggc aaagtacacg
    25921 aaaccgtaca aaccccctac cccaaacgcc gtctgaagca ttttatgtac cattacacat
    25981 acgacaactg ggaacaatat ttcaacaaat tcaacaaata cacttccatc tcagccgaga
    26041 aataccgaga gcagggaaag cccgtgcgtt tcgttaggga cattatcctc cgcccgattt
    26101 ggggtttttt caaaatttat atcctgaaca aagggtttct cgatggaaaa atgggttgga
    26161 ttatgtccgt caaccacagc tattacacga tgattaaata tgtcaaacta tattatctgt
    26221 acaaatctgg cggaaaattt taaatggaaa aagaattcag gatattaaat atcgtatcgg
    26281 ccaagatttg gggcgggggc gaacagtatg tctatgatgt ttcaaaagca ttggggcttc
    26341 ggggttgcac gatgtttacc gccgtcaata aaaataatga gttgatgcac agacgttttt
    26401 ccgaagtttc ttccgttttc acaacgcgcc ttcacacgct caacgggctg ttttcgctct
    26461 gcgcgcttac ccgctttatc cggaaaaacc gcatttccca cctgatgata cacaccggca
    26521 aaattgccgc cttatccata cttttgaaaa aactgaccgg ggtgcgcctg atatttgtca
    26581 aacataatgt cgtcgccaac aaaaccgatt tttaccaccg cctgatacag aaaaacacag
    26641 accgctttat ttgcgtttcc cgtctggttt acgatgtgca aaccgccgac aatcccttta
    26701 aagcaaaata ccggattgtt cataacggta tcgataccgg ccgtttccct ccctctcaag
    26761 aaaaacccga cagccgtttt tttaccgtcg cctacgccgg caggatcagt ccagaaaaag
    26821 gattggaaaa cctgattgaa gcctgtgtga tactgcatcg gaaatatcct caaatcaggc
    26881 tcaaattggc aggggacgga catccggatt atatgtgccg cctgaagcgg gacgtatctg
    26941 cttcaggagc agaaccattt gtttcttttg aagggtttac cgaaaaactt gcttcgtttt
    27001 accgccaaag cgatgtcgtg gttttgccca gcctcgtccc ggaggcattc ggtttgtcat
    27061 tatgcgaggc gatgtactgc cgaacggcgg tgatttccaa tactttgggg gcgcaaaagg
    27121 aaattatcga acatcatcaa tcggggattc tgctggacag gctgacacct gaatctttgg
    27181 cggacgaaat cgaacgcctc gtcttgaacc ctgaaacgaa aaacgcactg gcaacggcag
    27241 gacatcaatg cgtcgccaac cgttttacca tcaaccatac cgccgacaaa ttattggatg
    27301 caatataaac tgctttcaga cggcatatgc cgtctgaaag cctttgatgc aacaaaccgc
    27361 caaattatat tcgttcattg gaaagaaaca ccccgaatcc atccttaaaa ataagaaaat
    27421 cccaatatcc cccgatatta cgcagcctat tggcaaagtt ttgcagcgtc ttccccggct
    27481 tgtgctgccg catcaagtgc tttgttacaa tgtatagtag actaacaaaa accagtac
//
LOCUS       DAXHTR010000006        25739 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.006, whole genome shotgun sequence.
ACCESSION   DAXHTR010000006 DAXHTR010000000
VERSION     DAXHTR010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25739
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.006"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            25..102
                     /locus_tag="ACFNML_000194"
                     /pseudo
     CDS             25..102
                     /locus_tag="ACFNML_000194"
                     /inference="COORDINATES: protein motif:HMM:NF016757.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(149..2122)
                     /locus_tag="ACFNML_000195"
     CDS             complement(149..2122)
                     /locus_tag="ACFNML_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin knob domain-containing protein"
                     /protein_id="HFC6391650.1"
                     /translation="MHPIETPDKTFHDGDGVSELGTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGDQTITGQKTFTAQTQFQSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHGANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAGGSSNLPVENTACHIQVIAGTKHGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLKANGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAANGLAVGTKQEDEIKRHVHKVFSHWVNHPHAA
                     ALGYEDHNERQRSALVSTWTDENLNDNGFLTPRPDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETVNAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRHRDSLLEALQD"
     gene            complement(2133..2693)
                     /locus_tag="ACFNML_000196"
     CDS             complement(2133..2693)
                     /locus_tag="ACFNML_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFC6391651.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQRRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            complement(2690..3744)
                     /locus_tag="ACFNML_000197"
                     /pseudo
     CDS             complement(2690..3744)
                     /locus_tag="ACFNML_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284601.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            complement(3758..4105)
                     /locus_tag="ACFNML_000198"
     CDS             complement(3758..4105)
                     /locus_tag="ACFNML_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFC6391652.1"
                     /translation="MDKELNPDTGDYTGRTVDTLQNAVYIRLMTPLGSWWADKTLGSL
                     LHLLQREKDLQRVSLLAEQYADEALQPIVKSGRADKITVRAEQPHDGRLILHIRVDTA
                     AGGFDYRHEVPVI"
     gene            complement(4205..4873)
                     /locus_tag="ACFNML_000199"
     CDS             complement(4205..4873)
                     /locus_tag="ACFNML_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFC6391653.1"
                     /translation="MSLSKLAKKTAQTAKNIGETLRAAFRGKITLVVSSEPIQRVQLS
                     GLADETLQDLEHLQEYGFASHPPDGSEAVVIPLGGNTSHGVIVCSQHGSYRIKNLKPG
                     ETAIFNHEGAKIVIKQGKIIEADCDVYRVNCKQYEVNAATDAKFNAPLVETSAVLTAQ
                     GQINGNGGMAVEGGDGATFSGDVNQTGGSFNTDGDVVAGNISLRQHPHTDSIGGKTLP
                     AEPA"
     gene            complement(4870..6015)
                     /locus_tag="ACFNML_000200"
     CDS             complement(4870..6015)
                     /locus_tag="ACFNML_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFC6391654.1"
                     /translation="MQNNSYGYAVSVRVGGKEHRHWERYDIDSDFLIPADSFDFVIGR
                     LGPEAAIPDLSGESCEVVIDGQIVMTGIIGSQRHGKSKGSRELSLSGRDLAGFLVDCS
                     APQLNVKGMTVLDAAKKLAAPWPQIKAVVLKAENNPALDKIDIEPGETVWQALTHIAN
                     SVGLHPWLEPDGTLVVGGADYSSPPVATLCWSRTDSRCNIERMDIEWDTDNRFSEVTF
                     LAQSHGRSGDSAKHDLKWVYKDPTMTLHRPKTVVVSDADNLAALQKQAKKQLADWRLE
                     GFTLTITVGGHKTRDGVLWQPGQRVHVIDDEHGIDAVFFLMGRRFMLSRMDGTQTELR
                     LKEDGIWTPDAYPKKAEAARKRKGKRKGVSHKGKKGGKKQAETAVFE"
     gene            complement(6005..7336)
                     /locus_tag="ACFNML_000201"
     CDS             complement(6005..7336)
                     /locus_tag="ACFNML_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFC6391655.1"
                     /translation="MSGWHTLLQDASYKGVGFDIEVVDESNGKALAEHARPFVQGIDL
                     EDMGMTGRQVQINAVFWGKGYAGRLKKLLDALEQPGGGVLVHPVWGRMHNMIAASWSY
                     RHEADYVDYAGIDITFREAAEAQEIFVFENAFLVELEALIADIDTYREAAIGFVDAVL
                     AVDAGVSALWGSALGIWSAASGTFGAVRRLFDLDKIAFPDRGGYSAAAFKNGSAKLFA
                     DISVMVDTGIRREAGLADNAMHHAGWSPRQRFDGAVAVADRAAAIPDNLLTGRFSDGL
                     QNRLNRLTAKQVQPVAQAVRLLSTSSLLSVATALIEAHGEEMTAPDLIEVNRAMRRRM
                     QAEIAALRAVQTAAAESGGLTANAVYTEAYQTAESLRAAAGRLNALVAAAINQKPPLI
                     VRQAPIDGTIHQIAHEFYGDMARAAELVRLNPHIHHPAFIKRGTLVNSYAK"
     gene            complement(7336..9507)
                     /locus_tag="ACFNML_000202"
     CDS             complement(7336..9507)
                     /locus_tag="ACFNML_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRTRC system protein F"
                     /protein_id="HFC6391656.1"
                     /translation="MANGNMKLSLVLTARDDGARRLLADTQRQLDRTAKSRAQLERQS
                     HTYALTGIRSEKQIQREIMLTQAAFNRLARSGKASQNDLARAAVATRNRIRELNAELK
                     QGTGFADKMGKIGRFGAAAVAGGAAAYTVLKPAMDNRKQLDENINRVSRQAFIEDNSK
                     SAAWIATEGAQQIKDLALELVEKNGGTHDKALDLISGMMTTSLNFAQTKNEAQAAYAF
                     ALASEGSGEDTAKLIKTLKDGGMSGKDLQLGLEHVLQSGLDGTFEVRDMVRELPSLLP
                     AAQQAGMNGVGGLDYLLSLLQSAANKSGSPAEAATNVQNLLSKTLSPYTIGRLKKMAN
                     PNDPKKGVDWIGSVVQGKQNGENAVQVLSRLADAMLVKDKQYQDYKKRAAAGDKTAAE
                     QANMLKGALLAQLLPDLQAKQGLLAATDMTQIREYMASLAGVTLDNGKIAKNNEARML
                     SAAAQQEQQESLAMLRESLTGTLVDMETSFKKLAAEYPNATLALQALTTAATAASAAM
                     LLTAGGGKGAGFLKDVGSKALVWGKASAGGVAAGATAAGGKLLSWGKSAGSGLMNNPA
                     LVKRAGLLGMLLYSESLGDGTLPKGLRGTKTTPEMINRLKNNGIRFEPAPKREQARGG
                     VPQYLAAPSAQPTDKMLSPLFSTQTAAYQAAIQQQTAAYQAALAQDTAAVTTGLAQVQ
                     SAMASASQTINTNVSLNIDGRVIANEVSRYQVAMFGRGAGQ"
     gene            9662..10186
                     /locus_tag="ACFNML_000203"
     CDS             9662..10186
                     /locus_tag="ACFNML_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391657.1"
                     /translation="MNMKKLISAICVSIVLSACNQQSKTAQAEEPVQSIQAADCTAPM
                     DITVEQYLINLEQAFKTQNVSTKIHNKNIVKTDCGYDLTLVMDFGAIALKLDEQQKIR
                     AISVGYILKTDGEKGQNLVNNAINGLHSIQAVLSLTTTDKLGESEAGKQLRQRIQSDR
                     SNSAKRRSGRWYFI"
     gene            complement(10288..10557)
                     /locus_tag="ACFNML_000204"
     CDS             complement(10288..10557)
                     /locus_tag="ACFNML_000204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391658.1"
                     /translation="MPKTVRRYGEPRRAVGGGAVKRSYLTAHKSYRQAVILMAKAGIG
                     ADAVRDMCHAELSAWFEDILSSLGIKTPKEEGVIVSKRLRKPEGK"
     gene            complement(10497..10862)
                     /locus_tag="ACFNML_000205"
     CDS             complement(10497..10862)
                     /locus_tag="ACFNML_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391659.1"
                     /translation="MITVKLTHGLTYNGKVVSELRLKPLTVGGELAAFALIDDLPELP
                     ENATKAELLQRDVLETLTYWSQQIEAQGIPSDILTAQWLMENLSTEDYHTVMAAQEDL
                     CLKPSAATASPDAPSAAEQ"
     gene            complement(10866..11243)
                     /locus_tag="ACFNML_000206"
     CDS             complement(10866..11243)
                     /locus_tag="ACFNML_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFC6391660.1"
                     /translation="MSDATYTGAVIMEVNGRDIEIVSIKPQTTTGRKPVKTMNRNGRV
                     NGYCDGVTEHKLSVTAAIPIDGTEIDWDNITKAKITIYPINDEGRRTSYLDCFTVDTS
                     EQYEVDNEARIDIEMIALHKIKE"
     gene            complement(11308..12717)
                     /locus_tag="ACFNML_000207"
     CDS             complement(11308..12717)
                     /locus_tag="ACFNML_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFC6391661.1"
                     /translation="MPHIDFDTIPGSIRVPGQYIEFNTRNAVQGLPQNPQKVLMVAPM
                     LTAGIQPALEPVQLFSDAEAADLFGQGSLAHLMVRQAFANNPYLDLTVIGIADHSAGV
                     QATATVTLSGTATAPGVVEITIGGKQVSTAVNTGETAATVAGRLKTAITAADVTVTAS
                     GSGVAVTLTAKHKGEIGNESGLTVSTGNTGLTYQANVFTGGAKNADIATALSKVAGKH
                     YHIICSPFSDDANAKALSNHITNVFNAIEQRGCIGVLGMSAALSTATTATGKINDGRI
                     TCAWYKGAVEPNGIIAAGYAAVLAFEEDPAKPLNTLEIKGLAVTPDAQWPWFAECNNA
                     LYNGLTPLTAVNNRVQIMRAVSTYTKSANNTDDPALLDITTIRTLDYVRRSVKERIAL
                     RFPRDKLSDRLLPKVKSEILDVLIKLDQAEIIENAEANKGKLVVARAQNDPNRVNAII
                     PADVVNGLHVFAGRIDLIL"
     gene            complement(12720..12917)
                     /locus_tag="ACFNML_000208"
     CDS             complement(12720..12917)
                     /locus_tag="ACFNML_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFC6391662.1"
                     /translation="MSKIKVTAADGLRVPTEHNPHEYIGQEPVEVDGNSLYYRRMIDD
                     GDLVVVEDAAPNTKTRNTKGE"
     gene            complement(12914..13564)
                     /locus_tag="ACFNML_000209"
     CDS             complement(12914..13564)
                     /locus_tag="ACFNML_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp37"
                     /protein_id="HFC6391663.1"
                     /translation="MITDIEQAITDRLKRGLGRMVRTVKSYNGEADDLAGQIHTLPAV
                     WVTYGGSKVEPASTGGVCGRYQDTAEFVVMVAARNLRNEQAQRQGGIDSREIGSNDLI
                     RAVRRLLDGQRLGFADSRGLVPKAVRAIANHVLVQNAAVSIYAVEYAIRFNTCGLEND
                     RYPERTDNPDDPNHIFTKYQGTLSEPWPDFEGLDGKIYDPQSADEIPVNLTLKDKQ"
     gene            complement(13554..13976)
                     /locus_tag="ACFNML_000210"
     CDS             complement(13554..13976)
                     /locus_tag="ACFNML_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protein Gp36 family protein"
                     /protein_id="HFC6391664.1"
                     /translation="MAYATVEDMVARFGELEVLQLTDRNNEGLIDREVAQTALVDATA
                     EIDAYLGRFRRPFEDLPPILVRLCCDIARYRLTAAQGVLITDEIRNRYKIDVLDLLRA
                     MAKGEVQLGVDDSGEEVAAGEDGIVFVNGKNKVFGRDH"
     gene            complement(13976..14374)
                     /locus_tag="ACFNML_000211"
     CDS             complement(13976..14374)
                     /locus_tag="ACFNML_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391665.1"
                     /translation="MAKTNNKPETAETAAPSFEDIKAELDAVQAELAAARNDVEMLTT
                     ALEKAEDDKKALSAELAELKVQHTQRAADALADSRDVMLVSTGADGKEFWRGGLLFDG
                     GWREVKRAEVGEAVWKAICAEPMLQRKAVE"
     gene            complement(14439..15347)
                     /locus_tag="ACFNML_000212"
     CDS             complement(14439..15347)
                     /locus_tag="ACFNML_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu-like prophage major head subunit gpT family
                     protein"
                     /protein_id="HFC6391666.1"
                     /translation="MDKSAILTAITAAFRKEFQTGLESVKPDYTAVAMTIPSNTATNT
                     YAWLGKFPQMREWVGSRQIEKMNKQAMSLDNKKFEATVGVARTDIEDDQVGMYRPMMA
                     AMGESAAALPDTLVWGLLKKGKTTVGYDGQYFFDTDHPVYEKSDGTGQNTPHSNLTTG
                     TDNDAPTFYVVDDTKTLKPLIFQNRTDSEFETKFDPSKSDRVFMEDEYLYGSRRRCNA
                     GFGLWQLMHMAEKTALTRENLAAIIVKMQKIKADGGYVLNVKPSLLVVPPELEDKARE
                     LLEADKINGTTNTFKGRLKLHVCVHL"
     gene            complement(15347..16408)
                     /locus_tag="ACFNML_000213"
     CDS             complement(15347..16408)
                     /locus_tag="ACFNML_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage protease"
                     /protein_id="HFC6391667.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKSETEQNPMKELLQQLFGLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAEGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            complement(16639..17055)
                     /locus_tag="ACFNML_000214"
     CDS             complement(16639..17055)
                     /locus_tag="ACFNML_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFC6391668.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKPLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            complement(17183..18508)
                     /locus_tag="ACFNML_000215"
     CDS             complement(17183..18508)
                     /locus_tag="ACFNML_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head morphogenesis protein"
                     /protein_id="HFC6391669.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDTYLTLAYKAPDGSLYTTDRGFDYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKETQELAGM
                     TRATVWLSDDTLVKQVDSREGQSFGTDLYAMLPDLIHEPEYIFYDKSRGKESFVLLGK
                     PLEGNEKYRLMAVLKYLPQYDSIFLDSFRRADEKEIVKSKKRYEMKKGY"
     gene            complement(18495..20063)
                     /locus_tag="ACFNML_000216"
     CDS             complement(18495..20063)
                     /locus_tag="ACFNML_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HFC6391670.1"
                     /translation="MAKKNNKTKIQKPEAALQTDVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            complement(20063..21685)
                     /gene="terL"
                     /locus_tag="ACFNML_000217"
     CDS             complement(20063..21685)
                     /gene="terL"
                     /locus_tag="ACFNML_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HFC6391671.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDALPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHAFLFSRLPEIIRSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIATANDVKVQVAGSGKKLRGLRHGPYRPDLTILDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWRGVKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            21697..22209
                     /locus_tag="ACFNML_000218"
     CDS             21697..22209
                     /locus_tag="ACFNML_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391672.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELAPGSFVYRLKTEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDVEVFSVGG
                     SRYGRLIDGY"
     gene            complement(22206..22712)
                     /locus_tag="ACFNML_000219"
     CDS             complement(22206..22712)
                     /locus_tag="ACFNML_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HFC6391673.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQTTARAWKRADK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     AEVEKKFG"
     gene            complement(22715..22912)
                     /locus_tag="ACFNML_000220"
     CDS             complement(22715..22912)
                     /locus_tag="ACFNML_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391674.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            complement(22909..23175)
                     /locus_tag="ACFNML_000221"
     CDS             complement(22909..23175)
                     /locus_tag="ACFNML_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391675.1"
                     /translation="MDFEFGFRTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            complement(23187..23525)
                     /locus_tag="ACFNML_000222"
     CDS             complement(23187..23525)
                     /locus_tag="ACFNML_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391676.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFHLEYPITNYDTFKRAVWQTLGAYYCVCNDGDGLEIASNRPDGYA
                     VRIVFGDVPV"
     gene            complement(23522..23740)
                     /locus_tag="ACFNML_000223"
     CDS             complement(23522..23740)
                     /locus_tag="ACFNML_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391677.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            23753..23923
                     /locus_tag="ACFNML_000224"
     CDS             23753..23923
                     /locus_tag="ACFNML_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFC6391678.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            complement(23968..24273)
                     /locus_tag="ACFNML_000225"
     CDS             complement(23968..24273)
                     /locus_tag="ACFNML_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391679.1"
                     /translation="MSLLKTVKMQAAVVLTALAITACSSTSAPSATEIKVVEKAVMPT
                     PPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQNQAWRDWAGNHSRKVGN"
     gene            complement(24170..24589)
                     /locus_tag="ACFNML_000226"
     CDS             complement(24170..24589)
                     /locus_tag="ACFNML_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391680.1"
                     /translation="MNLVKLLANNWQPIAIIALVGTGLAVSHHQGYKSAFAKQQVVIE
                     KMKRDKAQAMLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAEVSRLKTENKK
                     EIENVLTQDRKNAGGGCIDGFGHHGLQLYKRALGYGN"
     gene            complement(24561..24797)
                     /locus_tag="ACFNML_000227"
     CDS             complement(24561..24797)
                     /locus_tag="ACFNML_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391681.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSLVSVKRQEVENESRETASE"
     gene            complement(24800..24964)
                     /locus_tag="ACFNML_000228"
     CDS             complement(24800..24964)
                     /locus_tag="ACFNML_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391682.1"
                     /translation="MGQVAFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            complement(24967..25173)
                     /locus_tag="ACFNML_000229"
     CDS             complement(24967..25173)
                     /locus_tag="ACFNML_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391683.1"
                     /translation="MNIIGKLKEAASYFLTKLIGENPSNEQVNRALIQMPNVRPIHTY
                     PRPNLRNSGVAAAKRAARKRKNRR"
     gene            complement(25170..25712)
                     /locus_tag="ACFNML_000230"
     CDS             complement(25170..25712)
                     /locus_tag="ACFNML_000230"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6391684.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGHHSRLAYAQAGGIVEPFFISNDTDLALFKTTK
                     WGICRAIADAIAMKLGAARV"
BASE COUNT         5274 a         7312 c         6644 g         6509 t
ORIGIN      
        1 atctctacag ccatctctac agccatctct acaacccatc tctacaaccc gtctctacag
       61 ccatctctac agccatctct acagccatct ctacagccat ctctacagcc atctctacag
      121 ccatctctac agccatctct acagcccgct agtcttgcag tgcctcgagc aagctgtctc
      181 ggtggcggcg gtttcggggg ccggttgatg ttcttccact ctctctttgc ggttcttcgt
      241 tggtttcagg tatttcgggg cctatcgggg agctggcggc gtttcccgag gcggctttgc
      301 cgatggccat ccagtcgcaa ctgccttcgt accagttggt aataatggcg gcggcgtggt
      361 tttttgcgcg gataaacagc cagttgtgtt gccttcgatc gccggtaacg cgtcccgaat
      421 ggcgttcgga tacgaagcat ttgacgttgc cgtcggcaaa ggcgacgggg aagacgactt
      481 cgttttctat ggggccgccg cttcgtgtga acctgtagct gccggtctgt atcattgtgc
      541 cgtcggggaa tctgacaata tccacgccgc cgatggtttg gcgtgtgaat tgcgcggcaa
      601 cggcggtgct gattttttcg tccagccctt ggatttgggc ggcggtgtgg gtgtgggcgc
      661 ggtctgcttt gtcttggagg ttttgcgcca gcgtgcccgc gtccagcgcg cctgcgttga
      721 cggtttcgcc gtgggacttt atccagaaca cggcttcgcc caaggtgtcg gcggctttga
      781 tgcacagttt taaaaccagg gctttggggc ggttttcgtc gccgcctgtc gccattttgc
      841 tgtccgggcg cggggttaaa aagccgttgt cgtttaaatt ttcgtccgtc caagtcgata
      901 cgagcgcgct tctctgcctt tcgttgtggt cttcgtaacc gagggctgcc gcgtgcgggt
      961 ggtttaccca gtgtgaaaat actttgtgga cgtgccgttt gatttcgtct tcctgcttcg
     1021 ttccgactgc caagccgttg gcggcgttgc ggataaagcg gtcttccgcc tgcgggacgt
     1081 tttggatgct gccgtatttt ccggtcagca gacggtacag ctcgggataa gcggtttcgg
     1141 ttacgcgcgt gcggatgtcg tcaaacgcca gccagccggt cgggatttgg tcggacggaa
     1201 accatgcggt gatgccgatg tcggtacggc ttaaatcggg caggcggttg ctgttgccca
     1261 gggcgcggta gaggtcgggg aaggtgtttt gtgcaaaggt cgtgccgttg gctttgagat
     1321 agcctgcggg gtttcggacg gctttgggaa aggatacgat cgcgccgacg gggatgccgt
     1381 ccgaattgag ttttttccat gcggaccagt tgtcgtttgc gctgctcgtt tggtggcgtt
     1441 cgtacacgtc ggacgtgtag gcgggataac ccaactgcct gcaccagccg tgtttcgtgc
     1501 cggcgatgac ttggatatgg caggcggtgt tttcaacggg caggttggag ctgccgcccg
     1561 ccgtcggcag ggaatagatg ccgtctgttt tgagggtgtt caaatcgccc cggaaggttt
     1621 cgactttgaa attgccgatg ccgtatccgg acagtgtgtt cggcttacct tcgatgtctt
     1681 cggcaaaacg cggtttgttg gctttgtgga agatgtcgtg gttgttgtag gaaatgcggt
     1741 tgtttgcgcc gtgcaggcgg agggtgtcgt cgccgaagac gatgtgggcg ttgtcggcat
     1801 ccgcgccgat gtaggctttg tggccgccgt tccagttcgt ctggttggcg gataaatgga
     1861 tgccgctttg gaattgggtt tgggcggtaa aggttttttg gccggtgatg gtttggtcgc
     1921 cggtggtaac tacagccagc ctatttagtg ctgccagtaa ttgattaggc tgcgacttat
     1981 ccggctggat accggccgca gtcagcacgg ccagcagctc ggattgcact tggttgagcc
     2041 accacgcggg caggatggtg cccaattcgg acacgccgtc gccgtcgtgg aaggtcttgt
     2101 cgggggtttc gatggggtgc atggtttagt ccttatcggt aggtaaatcg gatagcggtg
     2161 tgggcgggct tgaggcggtt gaacaggctc tcgatcacgg catcgctgta atcggtcagc
     2221 ctgtcgcccg ccgccgagat accggcgcgg aatcgggtaa tgcggttgtt gccgccgcgc
     2281 acgttaacgt gccacaccca catgatttcc tgcggcgcaa gacggtcgcc ggcgcggttt
     2341 acaccggcgc ggaacggctg cggttcgtcg atttggattt gatagcccgc cgcctcggcc
     2401 aaacgcacaa aataaggaat actcaagccg cctgtttcgt ttagcttggc catgacggcc
     2461 aacacacggc gctggcggtt tttgcccgta ccgtccaaac ctaatacgcg ctcccaatcg
     2521 gccagcattt ggccggcgct gcgcgggtcg acggcatcgg caacgctttg agccgattcc
     2581 gccaccgcat ccagcgctgc gccgtctatt tctgcctgcg cccgcacacg cggggcattg
     2641 cgggcatacg acacgggggg caacaggccc cgcaagatgt cttgatagct catgacggca
     2701 tctccgttac attaatcgcg cccgctttaa accactcgat gcggttaacc gtatcggcgg
     2761 cacggttggc cgtcggcgca gtcagacggc ggtcgatcac accatccaca ttgctgatag
     2821 ccgcctcgat ttgcgacaca gtcaggccgt cgccggggat cagggtgtcg aaatattctt
     2881 ttagggccgt ccgtatgcgg cgcttggcct cgtccaagtc gatgccgtcg agcttgactt
     2941 gcacggtaac aggcaccgcc gttacggttg gcttgagtac cagcgcattt tttgccgtta
     3001 ccgggcgcat ctcgtcgata taagcctgta cgcggcgcac agtttcttcc gatggcacac
     3061 cgtcggcgga ggtaatggca atatccaccg tacccaagcc gcggcgcagc ggataaacat
     3121 atgcgctggt tacgccgtca acactcaacg cccagttttt atagtcgtaa cggttgccgc
     3181 ctgcgggcgg tcggcggatg atttccaaca gacgcgccag cagtgaggca tcgctttctc
     3241 ggtcggtgcc gccttgtacg gtaaggcggc attcggtggc cacaccggcg ggggcggcca
     3301 tcagttgcgc ctcgccgtcg ccacattggc ggccgcgccc ggctcgtcgg cgattgccgg
     3361 tatttccgcc gtgccgccgc tgccgataac ggcgcgggcg gtagtgcggt aaaaacgctg
     3421 gccgatacgc acttgcaggc cgtctgaaag catggattgc gcaataccgc ttacggtcag
     3481 cgtgccgctg gccgtggtag gattgcggcg gcgcaagccg cgcatggagg catgccgctc
     3541 caaatactcg cggtcggcgg tatcagggaa aatctgccgc acaatccagc tttgatgcgc
     3601 atattgccct tcggcgcagc tggccaaacg gctggcgtgc acataatggt cgctgtcggg
     3661 gctgatatcg gcatccggcc acaggctttt ggtatcgcgc aggatacgct cgcggatttg
     3721 ctcaaatgtc ggcgtttcaa acacgtttaa aacctcttta aatcacgggc acttcgtggc
     3781 ggtaatcaaa cccgcccgcc gccgtatcca cccggatatg caggatcagg cggccgtcgt
     3841 gcggctgctc tgcgcgcacg gtaatcttgt cggcacgccc gctcttaaca atcggttgca
     3901 gtgcctcatc ggcatattgc tcggccaaca ggctgacccg ttgcaggtct ttttcgcgct
     3961 gcaacaaatg cagcagcgag ccgagcgttt tatccgccca ccagctgccc aacggtgtca
     4021 tcaaacggat atacacggca ttttgcagcg tatcgacggt gcggccggta tagtcgccgg
     4081 tgtcggggtt tagctctttg tccatgttgg cattttgccg ttttcagacg gcctcacgga
     4141 gtacacgggc ttcaatcccg cacgcacaaa aaagcagccc gaaggctgcc aaaggtctgc
     4201 ttgtctatgc cggttccgcc ggtaaggttt tgccgccgat gctgtcggta tgcgggtgct
     4261 ggcgcaacga tatattgccg gccaccacgt cgccgtcggt gttaaagctg ccgcccgttt
     4321 ggttaacatc gccgctaaag gtggctccgt cgccgccctc gacggccatg ccgccgttgc
     4381 cgttgatttg gccttgcgcc gtcaacactg cactggtctc caccaacgga gcgttaaatt
     4441 tggcatccgt ggccgcatta acctcgtatt gtttgcagtt aacccggtac acgtcgcaat
     4501 cggcctcaat gattttgcct tgcttaatca cgatttttgc accctcatga ttaaaaatcg
     4561 ccgtctcgcc gggcttaagg tttttgatgc ggtagctgcc gtgctggctg cacacaatca
     4621 caccgtgcga agtattgccg cccagcggta tcactaccgc ttcgctgccg tcgggcggat
     4681 ggctggcaaa gccgtattcc tgcaaatgtt caaggtcttg cagggtttcg tcggccaagc
     4741 cgctcaactg cacgcgctgt atcggctcgg acgacaccac cagcgtgatt tttccccgaa
     4801 aggccgcgcg cagggtttcg ccgatatttt tagcagtttg tgccgttttt ttcgccaatt
     4861 tactcaaact cattcaaaca ccgccgtttc tgcttgtttt ttgccgcctt ttttgccctt
     4921 atggctcacg cctttgcgtt tgcctttgcg cttgcgcgcc gcctcggcct ttttggggta
     4981 agcgtcgggt gtccaaatac cgtcctcttt gagccgcagc tcggtttgcg tgccatccat
     5041 gcgggatagc ataaaccgcc gccccatcag aaaaaacacc gcatcgatac cgtgctcgtc
     5101 gtcgatcaca tgcacacgct ggccaggttg ccacaatacg ccgtcgcggg ttttatggcc
     5161 gcccacggtt atcgtgagtg taaatccctc cagccgccag tcggccagct gctttttagc
     5221 ctgcttttgc aatgcggcca aattgtcggc atcggacacc accaccgttt tagggcggtg
     5281 cagcgtcatc gtcgggtctt tgtacaccca ctttaaatcg tgtttggcgc tgtcgccgct
     5341 gcggccgtgc gattgcgcca aaaaagtaac ctcggaaaag cggttgtcgg tatcccactc
     5401 aatgtccatg cgctcgatat tgcagcggct gtcggtgcgg ctccaacaca atgtcgccac
     5461 cggcgggctg ctgtaatccg caccgcccac caccaacgtg ccgtccggct ccagccacgg
     5521 atgcagcccg accgagttgg caatatgggt taatgcctgc catacggttt cgcccggctc
     5581 gatgtcgatt ttgtccaaag cggggttgtt ttcggcctta agcaccaccg ctttaatctg
     5641 cggccacggc gcggccagct ttttggctgc atccaatacc gtcatgccct ttacattgag
     5701 ctgcggcgcg gagcaatcca ccaaaaaacc ggccaaatca cgcccgctca agctcaactc
     5761 gcggctgccc ttgcttttgc cgtggcgctg gctgccgatg atgcccgtca tcacgatttg
     5821 cccgtctatc actacctcgc agctctctcc gcttaaatcg ggtatggccg cctccggccc
     5881 caacctgccg atgacaaaat cgaagctgtc ggcagggatt aaaaagtcgc tgtcgatgtc
     5941 gtagcgctcc cagtggcggt gctctttacc gcccacgcgc accgacacgg catagccgta
     6001 tgaattattt tgcatagctg ttgaccaaag tgccgcgctt gataaacgcg gggtggtgga
     6061 tatggggatt gagccgcacc agctctgctg cgcgggccat atcgccgtaa aactcgtggg
     6121 cgatttggtg tatcgtaccg tcgattgggg cttggcgcac aatcagcggc ggcttttggt
     6181 tgatggccgc cgcaaccaac gcattcagac ggcctgccgc cgcgcgcagg gattctgccg
     6241 tttggtaagc ctcggtatac acggcgttgg ccgtcagccc accagactcg gcagcagccg
     6301 tctgcaccgc ccgcaaggcg gcaatctcgg cctgcatacg gcggcgcatg gcgcggttaa
     6361 cctcaatcaa atcgggcgcg gtcatctctt cgccatgcgc ctcgattaat gccgttgcca
     6421 ccgacaacag cgatgacgtg gataacaggc gcaccgcctg cgctaccggc tgcacctgtt
     6481 tggcggttaa ccggttcagg cggttttgca ggccgtctga aaagcggccg gtcagtaaat
     6541 tatcagggat agcggcggca cggtcggcaa cagccacagc cccgtcaaac cgctgtcgcg
     6601 gcgaccaacc ggcatggtgc atggcattat cggccaaacc cgcctcacgg cgtatgccag
     6661 tatctaccat gaccgatata tccgcaaaca gcttggccga gccgttttta aacgccgctg
     6721 cgctgtatcc gccccgatcg ggaaaggcaa ttttgtccaa atcaaacaaa cggcgcaccg
     6781 caccaaacgt acccgatgcc gcactccaaa tgcccagcgc gctgccccat aaagccgata
     6841 cgcccgcatc caccgccaac accgcatcaa caaagccgat agccgcctcg cggtaggtgt
     6901 cgatatcagc aatcaacgcc tcaagctcga ccaaaaaggc gttttcaaaa acaaagattt
     6961 cctgcgcttc ggccgcctcg cggaaagtaa tatcgatgcc cgcataatcc acataatcgg
     7021 cctcatgtcg gtaactccat gatgccgcaa tcatgttgtg catccgcccc caaacagggt
     7081 gcaccagcac gccgccgccc ggctgctcca gcgcatccag cagctttttc agacggcctg
     7141 catagccctt gccccaaaac accgcattaa tctgcacctg ccgcccggtc atgcccatgt
     7201 cttcaaggtc gataccctgc acaaacggcc gcgcatgctc ggccaatgcc ttgccgttgc
     7261 tctcgtccac cacctcaata tcaaagccga cgcccttgta agatgcgtcc tgcaataagg
     7321 tatgccatcc gctcattatt gacccgctcc acggccgaac atggccactt gataccgcga
     7381 tacctcattc gcgataacac gtccgtcgat attcaggctc acattggtat tgatggtctg
     7441 acttgccgac gccatcgcac tttgcacttg tgccaaacct gttgtaactg cagccgtatc
     7501 ctgcgccaat gctgcctgat acgccgccgt ctgctgctga atggctgcct gatacgccgc
     7561 cgtctgagtt gaaaacaacg gagacaacat cttatcggta ggctgcgctg acggagcagc
     7621 caaatactga gggacaccac cccgcgcctg ttcccgcttc ggcgcaggtt caaatcggat
     7681 accgttgttt ttcagacgat taatcatttc aggagttgtc ttggtaccac gcaaaccctt
     7741 tggcaatgtg ccgtcaccca aagactcgga atacagcaac atacctaaca aacccgcccg
     7801 tttaactaac gctggattat tcatgagccc gctaccggca gattttcccc atgacagcaa
     7861 cttaccgcct gccgctgtgg cacctgctgc cacgccgcct gcggaagcct taccccatac
     7921 caacgcttta ctacctacat ctttcagaaa gcctgcacct ttaccgccac cggcggttaa
     7981 taacattgcg gcagacgccg ctgttgccgc cgtcgtcaat gcttgcaggg ctagagtggc
     8041 attagggtat tccgctgcca gctttttaaa cgaggtttcc atatccacca atgttcccgt
     8101 cagactttcc cgcaacattg ccagcgattc ctgttgctct tgttgcgccg ctgccgacaa
     8161 cattcgcgcc tcgttgttct tagcaatttt tccgttatcc aacgttacgc cagccaacga
     8221 agccatatat tcacggattt gcgtcatatc cgttgcagcc agcaaacctt gttttgcctg
     8281 caaatcaggc agcagttgcg ccaaaagcgc gcccttaagc atatttgcct gctccgccgc
     8341 cgtcttatcg cctgcagccg cgcgtttctt ataatcttgg tattgcttat cctttactag
     8401 catggcatcg gcaagacggg acaacacctg cactgcgttt tcgccgtttt gcttgccttg
     8461 cacaaccgag cctatccaat cgacaccttt cttcgggtca tttggatttg ccatcttctt
     8521 cagacgacct atcgtgtaag gcgacagagt tttactcaaa agattttgca cattagtcgc
     8581 cgcttcggca ggactgcccg atttattcgc cgcagattgt aagagtgaga gcaggtagtc
     8641 caaaccgccg acaccattca tccctgcctg ttgcgcggca gggagcaggc tcggcagctc
     8701 ccgaaccata tcccgcacct cgaaagtgcc gtctaaaccc gattgcaaga cgtgctcaag
     8761 cccgagttgc aggtctttac cgctcatgcc gccatctttc agggttttaa tcagttttgc
     8821 cgtatcctcg ccactgcctt ctgaggcaag tgcaaaagca tatgccgcct gcgcttcatt
     8881 cttggtttgg gcaaaattca gactggtggt catcatgccg ctgattaaat ccaaagcctt
     8941 atcgtgggtc ccgccatttt tctcgacaag ttcaagtgcc aaatccttga tctgttgcgc
     9001 accttcagtt gcaatccacg ctgccgattt actgttatcc tcaataaatg cctgtctgga
     9061 cacgcggttg atgttctcat caagctgctt tctgttgtcc atagcaggct taagcaccgt
     9121 atacgctgcc gcgccaccag ccaccgcagc tgcaccgaat cttccgattt ttcccatctt
     9181 gtccgcaaat cccgtgccct gtttcagttc cgcgttcagc tcgcgaattc ggttacgcgt
     9241 agcgaccgcc gcccgtgcca aatcattttg tgatgccttg ccgctgcgcg ccaaacggtt
     9301 aaacgcagcc tgtgtcagca tgatttcgcg ttgaatctgt ttttctgagc ggatgccggt
     9361 caacgcataa gtatggcttt gccgttcaag ttgcgcccgc gatttcgcgg tacgatctaa
     9421 ttgtcgttga gtatcagcca gtagccgtct cgctccgtca tctcgggcgg ttaacaccaa
     9481 cgacagtttc atgttcccgt ttgccataaa aagcagcctt taaacgtatt aactcaaggt
     9541 caattatcgt ttaaaggctg tttaagggag gcggtatggg cttcaacccc gttttcgtgc
     9601 tttatttgtt tgttgcgtta taataccaga atgataacca ttttattcaa agggatattt
     9661 tatgaatatg aaaaaattga tttccgcaat ttgtgtttca attgttttat cagcctgcaa
     9721 ccaacaatca aaaacggcac aagccgaaga acctgtccaa agtatccagg ctgctgattg
     9781 taccgcccca atggacatca cagttgaaca atatctcatc aatttggagc aagcatttaa
     9841 aactcagaac gtctcaacaa aaatccataa taaaaatatt gtcaagaccg attgtggtta
     9901 tgaccttact ttggtaatgg attttggggc gattgcgctc aaactggacg agcagcaaaa
     9961 aattagagct atctcagtag gctacatttt aaaaaccgac ggagagaaag gacaaaatct
    10021 agtcaataat gccataaatg gattacacag tattcaggca gttctgtctt taactaccac
    10081 agacaaattg ggcgaatcgg aagcaggaaa acaacttcgt caaagaatcc aatcagacag
    10141 gagcaacagc gcaaaaagac gttccggcag atggtatttt atatagcgtt gtttttgaaa
    10201 aagaaacaaa caccattgca ataatcggca gaaaacaacc ctaaccgtca aataaaaaaa
    10261 aggtcgtctg aaactcagac gaccttttta ttttccctcc ggctttctca gccgtttcga
    10321 cacaatcact ccctcttcct tcggcgtttt tatgcccagg ctcgacagga tgtcttcaaa
    10381 ccatgccgac agctcggcgt ggcacatatc gcgcactgcg tccgccccta tccccgcctt
    10441 tgccatcagg atgaccgcct gacggtagct tttgtgtgcc gtcaggtagc tgcgttttac
    10501 tgctccgccg ccgacggcgc gtcggggctc gccgtagcgg cggacggttt taggcacaaa
    10561 tcctcctgag ccgccatcac ggtatggtag tcttcggtag agaggttttc catcagccac
    10621 tgcgccgtca ggatgtcgga tgggatacct tgggcttcaa tctgctgcga ccagtaggtc
    10681 agcgtctcta ggacgtcgcg ttgcagcagt tcggcttttg tggcgttttc gggcagctcg
    10741 ggcaagtcat caatcagggc gaacgccgcc agttcgccgc cgacggtcag tggcttgagg
    10801 cgtaattcag atacgacttt gccattgtag gtcagcccgt gggtcagttt gacggtaatc
    10861 acgcattact ccttgatttt gtgcaaagca atcatctcaa tgtcgatgcg tgcctcgtta
    10921 tcgacttcat attgctcgct ggtatcgacg gtaaagcagt cgaggtagga agtgcggcga
    10981 ccttcgtcgt tgatggggta aatcgtgatt ttcgccttgg tgatgttgtc ccagtcgatt
    11041 tccgtaccgt cgatcggaat ggcggcggta acgcttaatt tgtgttcggt tacgccgtca
    11101 caataaccgt tgactcggcc gttgcggttc atcgttttga ccggcttgcg gcctgtagtg
    11161 gtttgcggtt tgatgctcac aatctcgata tcgcggccgt tcacctccat aatcaccgcg
    11221 ccggtatagg tagcgtcgct catgctttat ccttttttaa ggtttcagac ggcctttaaa
    11281 cctgttttaa acgccgtctg aaaagggtta caaaatcaaa tcaatgcgcc cggcaaagac
    11341 gtgcaggccg ttgaccacat cggcggggat aatggcatta acacggttgg ggtcgttttg
    11401 cgcacgcgcc accaccagct tgcctttgtt ggcctcggcg ttttcgatga tttcggcttg
    11461 gtcgagctta atcagcacgt ccaaaatctc gctcttaacc ttgggcagca ggcggtcgct
    11521 caatttgtcg cgcggaaaac gcagggcaat gcgctcttta acgctgcggc gcacataatc
    11581 cagcgtgcga atggtggtaa tgtcgagtag tgccgggtcg tcggtgttgt tggccgactt
    11641 ggtataggtt gatacggcac gcataatctg cacgcggttg ttgaccgctg tgagcggggt
    11701 caagccgttg tacagcgcat tgttgcattc tgcaaaccac ggccattgcg catcaggtgt
    11761 aacggccagc cctttgattt ccagcgtgtt cagcggcttg gcagggtctt cttcaaaggc
    11821 caacaccgcc gcataacctg cggcgatgat gccgtttggc tctaccgcac ctttgtacca
    11881 agcacaggta atgcggccgt cgttgatttt gccggtagcg gtggtggccg tgctcaaggc
    11941 cgcactcata cccaatacgc cgatacagcc gcgctgctcg atggcgttga atacgttggt
    12001 aatatggttg ctcaaggctt tggcgttggc gtcatcgcta aacgggctgc aaataatgtg
    12061 ataatgcttg cccgccactt tggacaaggc cgtggcaatg tccgcatttt tggcaccgcc
    12121 ggtaaagaca ttggcttgat aagtcaggcc ggtattgccg gtgctcacgg ttaagccgct
    12181 ctcgttgccg atctcgcctt tgtgtttggc cgtcagcgta acggctacgc cgctgccgga
    12241 tgcggtaacg gttacatcgg cggcagtgat ggcggttttc agacggcctg ccactgtggc
    12301 ggcggtctcg ccggtgttaa cggccgtgct tacctgcttg ccgccaatcg tgatttccac
    12361 cacgcccggc gcggtggccg tgccggaaag ggtaacggtt gcggttgcct gcacgcctgc
    12421 gctgtggtcg gcaataccga taacggtcaa atccaaataa gggttgttgg caaatgcttg
    12481 gcgcaccatc aaatgcgcca gcgagccttg tccgaacaaa tcggccgcct cggcatcgct
    12541 aaatagttgc accggctcta aggcgggctg tatgcccgcg gtcagcatgg gtgcaaccat
    12601 caataccttt tgcggatttt gcggcaaacc ttgcacggca ttgcgggtgt taaattcaat
    12661 atactgcccg ggcacgcgga tgctgcccgg aatcgtgtca aaatcaatat ggggcatcat
    12721 tactctccct tagtattgcg ggttttggta tttggggcgg catcctcaac caccaccaaa
    12781 tcgccgtcat caatcatgcg gcggtaatac aggctgttgc cgtccacctc caccggctct
    12841 tggccgatat attcgtgcgg gttgtgttcg gtcggcacac gcaggccatc tgccgccgtt
    12901 actttgattt tgctcattgc ttatccttaa gggttaggtt tacaggtatt tcatcggcgg
    12961 attgcgggtc gtaaattttg ccgtccaacc cctcgaaatc aggccacggc tcgctcaatg
    13021 taccctgata cttggtaaag atatggttgg ggtcgtcggg attgtcggtg cgttcggggt
    13081 agcggtcatt ttccaacccg caggtgttaa agcggatggc atactcaacc gcatatatgc
    13141 ttactgcggc gttttgcacc agcacatgat tggcaatcgc gcgcaccgct ttgggcacca
    13201 agccgcggct atcggcaaaa ccgagccgct ggccgtcaag caggcggcga acagcgcgga
    13261 ttaaatcgtt gctgccgatt tcgcggctgt cgatgccgcc ttgccgctgc gcctgctcgt
    13321 tgcgcagatt gcgggccgcc accatcacca caaattcggc ggtatcctga taacgtccgc
    13381 atacgccgcc ggtgctggca ggctcaactt tgctgccgcc atacgttacc caaaccgcag
    13441 gcagcgtatg gatttgcccc gccaaatcgt cggcctcgcc gttgtagctt ttaaccgtgc
    13501 gcaccatgcg acccaagccc cgtttcagac ggtctgttat cgcttgctca atatcagtga
    13561 tcacgcccga acaccttatt tttaccgttt acaaacacaa taccgtcttc gcccgcggcc
    13621 acttcttcgc cgctatcatc cacgcccagc tgcacttcgc ctttggccat agcacgcagc
    13681 aggtcgagca cgtcgatttt gtagcggttg cggatttcgt cggtaatcaa cacgccctga
    13741 gccgccgtca gacggtagcg ggcaatgtcg cagcaaaggc gcaccaagat gggcggcaga
    13801 tcctcaaaag gtcgtctgaa ccgccccaga tacgcgtcga tttcggcagt ggcgtccacc
    13861 agcgcggttt gtgcgacctc gcggtcaatc agcccctcgt tgttgcggtc ggtgagctgc
    13921 aagacttcca gctcaccgaa acgcgcaacc atatcctcaa ccgtcgcgta tgccattact
    13981 cgaccgcctt gcgttgcagc ataggctcgg cgcagattgc cttccacacc gcttcgccga
    14041 cttcggcgcg cttcacttca cgccagccgc cgtcaaacag caggccgccg cgccaaaatt
    14101 ctttgccgtc tgcgccggta ctgacgagca tcacatcgcg gctgtccgcc aaagcgtcgg
    14161 cggcgcgttg cgtatgctgc actttgagtt cggcaagttc ggcggacagt gcctttttgt
    14221 cgtcttcggc tttttccaag gctgtggtca gcatttcgac atcgtttcgg gcggcggcaa
    14281 gctctgcctg cacggcgtcc aattcggctt tgatgtcttc aaacgacggg gcggcggttt
    14341 cggcggtttc tggtttgttg ttggtttttg ccatgatgac tccttgtttc agacggcctt
    14401 taaataggtg ttaaaggtcg tctgaaaacg gttcggggtt acaggtggac gcaaacgtgc
    14461 agtttcagac ggcctttgaa ggtattggtc gtgccgttga ttttgtcggc ttccaataat
    14521 tcacgggctt tgtcttccag ctcgggcgga acgaccaaga ggctgggctt cacattcaac
    14581 acatagccac cgtcggcttt gattttctgc attttgacga taatggcggc gaggttttcg
    14641 cgggtcagcg cggttttttc cgccatgtgc atcagttgcc acaaaccgaa accggcattg
    14701 cagcggcggc gcgatccgta caggtattcg tcttccataa acacgcggtc ggatttggac
    14761 gggtcgaatt tggtttcaaa ctcggagtcg gtgcggtttt ggaaaatcag cggcttcagg
    14821 gtcttggtgt catcgacgac gtagaaggtc ggcgcgtcgt tgtccgtacc ggtcgtcaga
    14881 ttggagtgcg gcgtattctg acccgtgccg tccgatttct cgtaaacggg gtggtcggta
    14941 tcgaagaaat attggccgtc ataaccgaca gtggttttac cttttttgag cagaccccac
    15001 accagcgtat cgggcagggc ggcggcggac tcgcccattg ccgccatcat cgggcggtac
    15061 atgccgactt ggtcgtcttc gatgtccgta cgcgctacgc cgacggttgc ttcaaacttt
    15121 ttgttgtcga ggctcatcgc ctgcttgttc attttttcga tttggcgcga gccgacccat
    15181 tcgcgcattt gggggaattt gccgagccac gcataagtat tggttgccgt attggacgga
    15241 atggtcatcg caacggcggt atagtcgggc ttgacggatt ccaaacccgt ttgaaattct
    15301 ttgcggaatg ctgccgtgat tgcggtcaaa atcgctgatt tgtccattac ttaccttcgc
    15361 tttctttgat ttttacaaat tcttcgccgg acatgcccag catttttgct gcggctgcct
    15421 cttccgcagt cagtgcggcg acgcgttcgt cgggtgcttt accgcccgtt tgcgagcctg
    15481 ccagtgcagc gaccggctgg gcgttttcaa taaagccggt caaaaatgcc aagccgcccg
    15541 gctgtttcaa tacgccttct gcccactcct tctgagcagg cagcaatttg cctgaagtca
    15601 gcgcggcggt aatcaattcg ttgcctttgt ctgcttcctg cttggcagtc agcgcggcga
    15661 ctttgctttg cagctcttga accactgaga taggcgcgta tttagtcaaa tcaggcttgg
    15721 cggtttgcgc cgtcaatgcc gcgatgcggc tgtctttttc cgccagctgc gcgaacacgt
    15781 cggcagacaa tgccacgtct ttcggcttgg cttccacgag cgcggacaat gccgccttca
    15841 gttcttcttc gcccgcatca ggcagaccga acagttgctg taacaactct ttcatagggt
    15901 tttgctccgt ttccgatttt aaaatttgcg ccgacgctgc cgccagcacc tcgtccatac
    15961 cgtccaacgc ggggaaattt gtcagcgcgg cgtgaaaaat tttgcttaca tatccctttg
    16021 tgtcatagga aaacacagca gagatgtagc gatactcttt tgcggcaatt gccgcagccg
    16081 ccttgtccgt ccactccact tcggcaaaca tgcctttagg cgtgaactcc agccaacgca
    16141 tccaaccggc ggcaggtgca ggttgtccgt ttttctcttt gtagagcgtc tggtgttcat
    16201 aatcgacaac caactgattg cgcgagctgt tggccaacaa cgcgacatca tgaccgtttt
    16261 cttcggtcag ataccacgca gggacatcag tcggacgacc gtctactgcg cgaaattcgc
    16321 catatggcag cagttggatt cgcccgtctt ttggctgcac ctcgaaactg cacacggcaa
    16381 gtagggtttt ttgtgcattt ttggacatgt gtacctctct caatacggtc attttgccta
    16441 tttgaaaaaa caacaaagag taggcgtgct tcaaaccctc ccgccatttt tttaaaacct
    16501 gtccgcgcga tacacaatta atcgttttca ggggcttttt agaagcgtct aggattgatt
    16561 tttatatctt gggcaggggt ttgtattacc cgcataaaaa aacgcgctaa aaagcgcgtt
    16621 tttgagggtt ttataaattc acggtatcag acccgaaaaa taatcctgca catcgtccat
    16681 caaagcctgt ttgtcgtcgt ccgtcagcgt caaaaattcc cgttgcggaa tccgaacttt
    16741 gcggttgcgc cccgccatac cgccgaagtt gtggatggcg gcatagacga tattcgtacc
    16801 gacaagggcg gtatcgttgt ctgacagtgt ggaaaaactg tctttcagac gacccgaatc
    16861 cgaaagcggc ttgccgtcgc ggtattttag ccccaaccat ttcggacggc ctgcgtagcg
    16921 gaaattgagc ttgaccgccg tgtgcatggt ttcggacagt cggcgcatca gcaggtagcg
    16981 gttttcgatc ccgttgccaa gccgctcgat ttggtttagg acgacaaaga tattgtctat
    17041 tttgacatca atcatggtat tatcctgcct aggctactaa aaaagcggtg aatttgtctg
    17101 ccctccgcag cccttatgca gtcctcggac tgggattggt atccgttaca taagcgagag
    17161 tgtgtcggga ttgcagtccg acttagtagc cttttttcat ttcatagcgt ttcttacttt
    17221 tcacaatttc tttttcatcc gccctgcgaa acgaatccaa aaaaatcgaa tcgtactgtg
    17281 gcagatattt caaaactgcc atcaatctgt atttttcgtt cccctccaac ggttttccga
    17341 gcaaaacaaa actttctttt cctctgcttt tgtcataaaa aatatattcg ggctcatgaa
    17401 tcagatcggg gagcatggca tataaatctg ttccaaacga ttgcccctcc cgactgtcca
    17461 cctgtttgac caatgtatca tcagacagcc acaccgtcgc tcgtgtcata cctgccaatt
    17521 cttgcgtttc cttgctcaat acacccgcag caaatttaag ctgtcttgat agcgcatttc
    17581 ggattttgat tttctgctct ttatcgggct tgccatcaat atccaaacgt tgcttgactt
    17641 cataaaactc tttttcaagc tgtttaaagc tggttttaaa atccgcacca cccatttccg
    17701 ctttggcaaa ttgatgcgcc aacgcacggt cgtacttgtc taaatcgggg cggtagttca
    17761 ttcgtccggc gttgtaatca aatcctcgat cggtcgtgta cagactgcca tccggtgctt
    17821 tataggcaag ggtcagataa gtatcgcctt ttttgttgta gattttatgg gtctcgacta
    17881 gattgtccgc cgtgctttgc ccgacaatcc gcccctggcg ttccacatcc cgctccgaca
    17941 gcgcgatgac cgagcaacgg cagttgtagc cgttgggcgg gtaaaacgtt gcccaaaacg
    18001 ggtcgtcgta gcggtacacc agcccgtcta ttgccgaatg cgccggacgg gtgcggctgt
    18061 cgcctaccgc gtcatacatc caatagggcc gtgcatcaat atttgccata tatccttgat
    18121 attgaccggc gttgtaggca gtttgcatgt tggtacggta aatcgtctcc aacctccgcg
    18181 gcgaaccgaa tacctcgccg gtggctgggt cgatgatatc cttaccgtta tgcccgttcg
    18241 gatgcagcca gcccttgttg ccgagcagat tcaagatacc ttttcgccaa tcgtcaaacg
    18301 actgtccact ttttgccgat tcaaccatcg aagttttgat gtcgttgagc atatccaagt
    18361 cggtcatgcg ggcaatcgta tagactttgg caatttcgga ggctgtcaga ttgcggtagc
    18421 tctccgccgt aaccttttta ctttccagcc actcgattgc cttttttggc ggcagactta
    18481 aggcaaatcc caaatcaggc gcgggcatgg tcttgtccca aaatatcgct gataaacaag
    18541 gcctgctgca tataggtacg cagtttcgcg ttgtccaaat tcggataaag cgcattcagt
    18601 gcggcatctg cctcctcgta gctgttgcaa gcattaagtg cggcaaccgc ctgacgcacc
    18661 atcgggttga gctgagaatt gaaatcgggc tcaaccagcg cgtcatccaa cgcgccgtcc
    18721 aatatttcct gatgcctgcc cgtagcctta gatggtacgg tgtgggcgga taaagccgcc
    18781 aatgcagttc tgtttatcgg attgtccggt acctgccgca ccaacacagc ctcaccctcc
    18841 tgcacatctg gaatgaccag tttgtcgcgc acccagcttt cggggatttg tacgccgaca
    18901 tccaccagtt tcgggatagc gtcggcaaag accgcgatgt ctttcggctc gcgcgtgtca
    18961 aattcaaatt tcggcacgcg gtttgggtcg gcattgggat agttgatttg caggaacggt
    19021 ccgatgattt ggcttgtgat ggtttgcgcc acctgttttg cgtccgacac cagcaaatcg
    19081 cggcgtacct cgttgtggat attgcccagc gcgttggtgc tggattttcc gtccgcaccg
    19141 ctggttagcg tttgccccaa aatcagccgc gccgccgatt tttcgcacca gtcggccatc
    19201 tgcaaaaacg gattgctggt tgccatcgta ccgcttgcag cgttgtgcag ctcgatttcc
    19261 ataccttctg gcatgatgcc tgccgcgttg tgaccgattt ccgccaccgc tcgaagcagg
    19321 gtgttttttt cctctttggt tgcgcccgcg ccgtatttgc cgatacggat gggcatgccg
    19381 tacagctcca aaaactcggc aaaatcgtgg acggcgtagt gtttgaacat atacagccag
    19441 gaaagcgtgc ggaaaagccc gttgcgcgcc tgctggacgc tgcgcgattt ttgggtatga
    19501 acgacccagc ccagcggcca caacgcttcg ccttccggat tttcgcgggt acgcagcagc
    19561 agcccgttgt ctttgtccca tttgaaccag ctttgcgggc ggtggataaa gtttcggggt
    19621 aggtaaaggc cgtctgaaaa tacccactcg acctccaacg cagaaaatcc gtgccctacc
    19681 gcgtccatca aatccataat caggtcttcg agggtaggca ggctgtccat catttcgtag
    19741 gcttggtcgg acagcttttc ttcttcgggc gtcgcatttc gcggcggggc gacgcgccag
    19801 ttgagcgtca gcagcgcgcg tttgcgcgtc cccatatttg ccgcgatgtc gctgtcgcgc
    19861 tcctcaatgt ccgcgaaaag ctcgtgttgg gcgcggatgt cgccgctttc cgcatcctcg
    19921 aagagggccc gcatcttttg cggtgtaata aaattggacg gatgctcggc gataacccga
    19981 ccggtcgccg taatttgagc cacgtccgtc tgcaatgcag cttcgggctt ttggatttta
    20041 gttttattgt tcttttttgc cattatcggc tccatttact ttttatctcg tcatcaaaat
    20101 cgttatcggt agggctttgc cattctatcg gcacacaatt ggccaccgcc cccgaccaga
    20161 gcatatgcac tgcatcagga ccatcatcat gatcagcctt tggaaaatgg cggaactgct
    20221 gtatcagcgt ctgttggctc tcattcaaca gaatcaaacc gttcgccatg tgaggctgta
    20281 aagtctcaat ccgcaagagc ttgtccgata ccggcttgac cgcccgcgcc gggacaggta
    20341 ttccacgcgc cgcgctgcgc ttgaccagct catctttcaa aaattcctga aactgaaccg
    20401 tctcaacaaa ccacagtttg caacgatatt gacggtgcaa tctgataacg tcctcaataa
    20461 tcaaatcagg gagacgtttt ttaatctgag cttccacgac atacagtttg ccggtttcac
    20521 gttgataacc gccaatgata atcgccgacg ggtcacggct cgcccccgct ttacccagcg
    20581 acgggtcgag cgcaccgaaa tacaccaaat cggacggcag gtcgttccaa aacttcatcg
    20641 acttggcaaa cggcgcatct tcgccgctga ccgggtcgtt ttgatactcg ctgtcaaatg
    20701 tcgcatgacc gtcacgggcg cggattttca tcagcgcgag tacgccgcga gccgcccagc
    20761 ttgtttgcgc gccgcgctcc atctcgtctt tattggcgag ataaaacgcc tcggccaccg
    20821 tctcgccgtc gtttcggaaa agttcctccc atctgtccca caagtccatg cggtcgggcc
    20881 agcgtttcat cgccttaaac ttaacacccc gccaaaacgg attattcagg gtgcggttta
    20941 gcacgctgtc gtaatgcaaa atcgtgccga tataaatcac atcgtatttc tgaccgacac
    21001 cgcccaaagg caatacagtc ttagtcagcc acgcattgag cttgtcgcgc tgttcggggt
    21061 tgcggacttg ctcgtcattc tcaatatcgt ccaatatggt caggtcagga cggtaagggc
    21121 cgtgacgcaa accgcgcagc tttttaccgc taccggccac ttggacttta acgtcattgg
    21181 ccgtcgcaat cgtaccggcc tgccatacgc ggccctgccc gcatacttcc ggaaagtcgg
    21241 ttttcaagcg cggattaaat tccaattccg ccttgatggc ttcgagcatc gggtatgcct
    21301 gatctatgct gtccatcaca ataacggcat aatgtttttg gcctgtcaca atacaccaca
    21361 gcgtaaacaa ctgagtaacc tgcgtcgact taccctcgcc acgcggcgca cccaccgcct
    21421 cattttcccc tttgggggag cggataatct ccggcagacg gctgaataaa aacgcatgca
    21481 gttcggattt ttcaggggag cggatataat gcggaaagta ggtgttgacg aaatactcgt
    21541 aaccgcctac cgggtcaaac accttggcac ggcgtgcagc aatagccttg ggcaacgcat
    21601 caaagccgtc cacttccgct tcgataactt ggcgcaaact ggcggccagt tcggcaaggg
    21661 atttgaggaa ttcttttgtt ttcatgagta tttataatga ctggattcat ttctaacgca
    21721 tttataggcc taaaagaagc aggatcactt ttaaaagggt ttaaccagct caaaacagag
    21781 gcagaggtag cgcaaaaaac catcgaactt aatcaaatca ttgctggtat tcaaactgat
    21841 ttatttgaag cccaatcttc ttatacggcg gcaattagcc gtatagatga gttggaaaaa
    21901 gaactggtgg cagtaaaaaa ttggtctaca gagaagcaac gctaccaatt gtatgaactt
    21961 gctcctggtt ctttcgtcta tcgattgaaa acagagatgg cgaacagcga gccaattcat
    22021 gatatttgtc cccaatgtta cgagcagggt gtcaaatcga ttcttcaatt caatgggtat
    22081 gaaggtgctt ttcataaata ttcctgttcc cgttgccaaa ccataatatt gggagagcgt
    22141 ttaaagtcag atgttgaagt attttcagta ggaggatcac gttatgggag attgatagac
    22201 ggctattaac caaacttctt ctccacttcc gccccaaacg gctccaatac ctccacaaag
    22261 gcaggcaaat gtttgggatg tttttcttgc acaaacgcca tcaagaactc aatcaattcc
    22321 aaagccgtcg ccagttttga cgtttccggc atcacacgcg cattggccga tacggttttc
    22381 gtaaacgcat cggccaggct ggccaacagc ttggcgcggt cggacggggg caaatcttcg
    22441 gtactcgaat cctgcagcat cgtcatcgtg ctgttgtact gcaccataaa accggccaac
    22501 atcgcacggc tcaagtcctc aataccgccg ccggccaaag tgtaggcggc gcgcatctta
    22561 tcccagtcgt cgcctttttc cttatccgca cgtttccacg cacgcgcagt ggtctgcggg
    22621 atttcgcaca tcaaggccgc cgtttccaaa gtttgctcgc cgctcacata gagccgacgt
    22681 aacttttcac ggatttcttg cgggtgagcc ataattacag tcccattttc gctttaagca
    22741 attcccagcc gaccgtaatc acgccgccgc ccagtgcgcc gaatgtaatg gccgtgcgtt
    22801 tcgtgtcttg gcggatttgc gcaatttccg cctgcatttc tttctgattt ttcagcgttt
    22861 gatcagtctt gttttcaata cgcgccaagg cttccaaaat cgggtcgctc atgatttgtc
    22921 tgctttcctg tccagttttt cgtttacttt ttccaacttg ttttcgatgc gtcccaacga
    22981 agccgcaata ttgtcgcggt ctgccttggc atcttgcttg gtgtgataag agagcttgac
    23041 cgcgtgcagc tcctctttca ggtcgtcgat acgcttgtcc gcctctttca gacggcctga
    23101 aatgccgttg acccaaaacc aaaatgccgc cgtcgcaatc ggccacaggg ttctgaaacc
    23161 aaattcaaag tccatttaaa acccctttaa accggcacat cgccgaatac gatacggacg
    23221 gcgtaaccgt cagggcgatt gctggcaatc tccagtccat caccatcatt acaaacacag
    23281 taatacgccc ccaaagtttg ccaaaccgca cgtttaaagg tgtcatagtt cgtaattgga
    23341 tattcaaggt gaaacgtcgt ctgaaaatcc ttatccatgc gtaccgcgta ctcaatgcct
    23401 gccttatcca acagatcgga aacatgaatg acaaacggct cttgctcgcg tgcgcggctt
    23461 aagcccaatt ccaaatcggc atggcgcaca gccaactgac gttcaactaa ttcacggtag
    23521 gtcattcttt gatacccatt aaatattttg tccgcttgta caatttctta acccacgaaa
    23581 tatttacaaa tgtataaacc tttgctacaa cttcgccgtc atagtgcgca ttttctcgtg
    23641 cagcccgaaa tttcgccctg gcttcttcag ggttgtccgc ccaaatgctc aatgaccagg
    23701 acttgccgtt aaagcggtaa gaaaacgtgt actcattcat aggagaaacc ttatgtattt
    23761 tgaaatctat aaagacgcaa aaggcgaata ccgttggcgt ttgaaagcag ccaaccatga
    23821 aatcatcgct cagggcgaag gctacaccag caagcaaaac tgtcagcacg cagtcgattt
    23881 gctgaaaagc actaccgccg ctacccctgt aaaagaggta taaaatccgc tttcaccctc
    23941 agcccgcgcc ctacgcgggc ttttttgtca gtttccgact ttgcgggagt gattgcccgc
    24001 ccagtcgcgc caagcctgat tttgattttc caactccgcc acatagccgc caaactcggc
    24061 ggcgtgttcc aacagcgtgg ccgtcttgcc gtctttcggc ggatttgggc gtaccggcgc
    24121 gaccatcaat gcggcaggcg gtgtcggcat gaccgccttt tcgacaacct taatttccgt
    24181 agccgagggc gcgcttgtag agctgcaagc cgtgatggcc aaagccgtca atacaaccgc
    24241 cgcctgcatt tttacggtct tgagtaagga cattttcgat ttccttttta ttttccgttt
    24301 tcagacggct gacttccgcc tgttttttcg ccaaagccat gccgacggcg tgcgccttga
    24361 cttcatattt tttcgcttcc gcgcgcgcct gttccagctc gcgtgcatag ttttgagccg
    24421 acaacagcat ggcttgcgcc ttgtcgcgct tcattttctc aatgaccacc tgctgcttcg
    24481 caaacgccga cttatagcct tgatggtgcg acaccgccaa acccgtgccg acaagcgcga
    24541 taatggcaat tggttgccag ttattcgcta gcagtttcac gagattcatt ctcgacctcc
    24601 tgacgtttga cgctgaccaa cgaacgcgcc accgcatagc cgccgacaat gcccaaatac
    24661 accgcccaaa tctccgccga tgggtcgggc aacatcacaa atttaaacgt ccccgccgcg
    24721 caggcgacgt ttgcccacag cttcgagtgc gacacattgc ctgtcgccgg gtttttaaaa
    24781 atatccaaaa tacgcattgc tattccacac ttttggtttg caggtgccgt ttcagcattt
    24841 cctgataatt ggccagttcg ccctccgcaa attcaaacgc cgccaagtct gcctgttcgc
    24901 ttgcctcacg gcttttggcc gaccacagcc caatcatctt ttcgtaaaac gcaacctgtc
    24961 ccatgattaa cgacgattct tgcgtttgcg cgcagcccgt ttcgcggctg ccacgcctga
    25021 gtttcttaaa tttgggcgtg gataggtgtg tatcggacga acatttggca tctgtataag
    25081 tgcgcggttt acctgctcat tactaggatt ttctccaatc aattttgtaa gaaaatagga
    25141 agcagcttct ttcaatttac caataatatt catactcttg ccgcccccaa tttcatcgca
    25201 atcgcgtccg caatcgcgcg gcagatgccc catttggtcg tcttaaacaa ggccaaatca
    25261 gtgtcgttgc tgatgaaaaa aggctcaaca atgccgccag cctgcgcata agccaggcgc
    25321 gaatgatggc ccgcattgtc gggtttaaag ccgtcttcgc cgcgcagttt ccagccggtt
    25381 ttcttggcaa cagctttgct caacacctga caccagcgtt tgtttttaac ggtactcaac
    25441 gcctcaatgc ccgtagctgc tttaccggcc gccgagttgg tatggaactc aatcgccaca
    25501 tccgagccgc gaatcagttt tacagcttca cgcagcggca tattgccttt gcctgtgccg
    25561 tcggttttaa cagtcaaacc gtagtcatcg cgcaaaatag cagccacaat gttgcgcata
    25621 tcctgcgcca agtccgcctc acggtcgctg ccgttgaccg cacccgggtc ggtgttgctg
    25681 tgtccagcgg ttaaggttac ggttttgccc attcatcatc tccaaaaaat attgattgc
//
LOCUS       DAXHTR010000007        25627 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.007, whole genome shotgun sequence.
ACCESSION   DAXHTR010000007 DAXHTR010000000
VERSION     DAXHTR010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25627
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.007"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            204..1046
                     /locus_tag="ACFNML_000231"
     CDS             204..1046
                     /locus_tag="ACFNML_000231"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFC6391685.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     NDRVAVIATLPLTRDESWSQLAVNELVMFREGNIVRHDRPENPVYMSAEEGLEIARAA
                     GVSV"
     gene            1075..1539
                     /locus_tag="ACFNML_000232"
     CDS             1075..1539
                     /locus_tag="ACFNML_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFC6391686.1"
                     /translation="MKCPFCAHPDTRVADSRLMEERNAVRRRRHCPNCGKRFGTLETA
                     ELKMPAVIGTDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTEHRLYTSGQRD
                     IPSTELADMVLKELLEHNTEAAVRFAALHKRFDNPSDFASWLAQAVKTDRKA"
     gene            1572..2681
                     /gene="ribD"
                     /locus_tag="ACFNML_000233"
     CDS             1572..2681
                     /gene="ribD"
                     /locus_tag="ACFNML_000233"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFC6391687.1"
                     /translation="MFSDTDISMMEIALRLAALGRFSTSPNPRVGCVIAHGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLAMLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPQLNVRDFPTLRQP
                     ARIVLDSRLRLPPYSHLVTDGQSPTLIATLERDEDKLRPYREHAHIRILMPSETADSK
                     IDLHHLMRLLADEGFGEIMVEAGSELTSAFLAENLADEIVLYRSPKILGSGKDLLSLP
                     ENRAALSAPPLWTPVSTEILGHDIKTVFRKNDNAF"
     gene            2876..4294
                     /locus_tag="ACFNML_000234"
     CDS             2876..4294
                     /locus_tag="ACFNML_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFC6391688.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSSQLVSKLAILLLLLTV
                     GLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIPI
                     ALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFRA
                     IEENAPPARLSATAEFAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPPL
                     FCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACATSFWL
                     FFAFKTESSCRLWQPLKRLPLYTHTLLCLTSSAAYTCFGTPANYPLFAGVWAAYLAGC
                     ILRHRKDLHKLFHYLKKQGFPL"
     gene            4291..5364
                     /locus_tag="ACFNML_000235"
     CDS             4291..5364
                     /locus_tag="ACFNML_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6391689.1"
                     /translation="MKIVLTTSMAGLGGTETATVRLGRLLRQHGHDIMLASSDGPLLK
                     EAQALGIRWQPVDFYRGGLFGYLKGMIAYARMLRREQPDIIDCQMARVVPACAIAAKI
                     VSPKTKIIYHSHGLDAATYPKIAKLFDKLGVYIIGNCKHEREKLIRHGFPADRIVYAY
                     NALPPPPEFPFWKTVKGCVMLGTLSRLDTVRAVHLMLDIFKKMVDRDMPVRLNVAGIG
                     EEMDNLKAQAERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILE
                     AGLYDTPVVTYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRL
                     HAHVAALCSDDEIYRTTMAAYTM"
     gene            5384..6553
                     /gene="pglH"
                     /locus_tag="ACFNML_000236"
     CDS             5384..6553
                     /gene="pglH"
                     /locus_tag="ACFNML_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HFC6391690.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLRVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAAIAPPPPRFRSKTTR
                     LFYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLEKLKQYENRAIKFYGYL
                     PYSEMMSIAKGCDIAVNAIHSHAMQSVTNKLSDYMALQKPILNSQTNAEVLSLLNLLP
                     HENYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFRRDISYRKIVNLIERLANE"
     gene            6546..7073
                     /locus_tag="ACFNML_000237"
                     /pseudo
     CDS             6546..7073
                     /locus_tag="ACFNML_000237"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            7122..8123
                     /locus_tag="ACFNML_000238"
     CDS             7122..8123
                     /locus_tag="ACFNML_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFC6391691.1"
                     /translation="MPYFTGNQTNEKNNILILSAGRRVELVQDFQTEAARFSDGISVF
                     ATDLNPRMSSACHVSDGSFEVPPISTDSYIDSIFNLAVRENIGLIIPTIDTELQKLAD
                     ERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRSPEIYDRKKLVFPCFAKPY
                     DGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFSEFTVDMYYDRQCRLKCAI
                     PRKRLEVRSGEISKGVTCKNSLYQTLLEKMAVLQGARGCITAQFFYNEETGEFYGVEI
                     NPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWENNLIMLRYDAKVLVHEND"
     gene            8110..8796
                     /locus_tag="ACFNML_000239"
     CDS             8110..8796
                     /locus_tag="ACFNML_000239"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6391692.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            8844..9038
                     /locus_tag="ACFNML_000240"
     CDS             8844..9038
                     /locus_tag="ACFNML_000240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391693.1"
                     /translation="MTKYYSWGVNGFPPYVFDTIWKRWKLNGRGGGYRVRHPGRHDRR
                     GQSGKAAAAQKPRDRDGISD"
     gene            9162..10337
                     /locus_tag="ACFNML_000241"
     CDS             9162..10337
                     /locus_tag="ACFNML_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFC6391694.1"
                     /translation="MLNTSLSPWPCFTQEEADAVSKVLLSNKVNYWTGNECRKFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAMGIGAGDDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAVLTPNTKAIIVVHLAGMPAEMDGIMALAKEHNLWVVEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKELWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHESFGTNWRMMEMQAAIGRIQLKRLPEWTVCRQAHAAKLAESLG
                     KFKSIRLVEVADYIGHAQYKFYAFVKPEHLKDGWTRDRIVNELNARKVPCYQGSCSEV
                     YLEKSFDNTPWRPKERLKNAVELGDTSLMFLVHPTLTDGEIAFCKEHIEAVLTEATR"
     gene            10385..12295
                     /gene="pglD"
                     /locus_tag="ACFNML_000242"
     CDS             10385..12295
                     /gene="pglD"
                     /locus_tag="ACFNML_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HFC6391695.1"
                     /translation="MTLETLLALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTAPLTIGLFVRMGLYRAVTRFISFRILSTALAGSLASAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGQAGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDNRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAVEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVETFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPAFEKQIAEGGPITLTHQDITRYFMTIPEAAQLVIQAGAMGKGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPKTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TGHPRIMTADEAMLPWHELSVLLDRIRAACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            12351..13643
                     /locus_tag="ACFNML_000243"
     CDS             12351..13643
                     /locus_tag="ACFNML_000243"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFC6391696.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDSEMARLD
                     ALAQEHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTR
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(13695..14531)
                     /locus_tag="ACFNML_000244"
     CDS             complement(13695..14531)
                     /locus_tag="ACFNML_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HFC6391697.1"
                     /translation="MIDLHCHSTVSDGMLSPAEVVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIAAKLEKRGISGAYDGALALAANKEMVSRTHVAEFLIRVGHVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGMAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYAFLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            complement(14927..15130)
                     /locus_tag="ACFNML_000245"
     CDS             complement(14927..15130)
                     /locus_tag="ACFNML_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase-like domain-containing protein"
                     /protein_id="HFC6391698.1"
                     /translation="MDMTLKHKAEALLGEPLLDEPVRPESWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWSDDAV"
     gene            complement(15108..15341)
                     /locus_tag="ACFNML_000246"
     CDS             complement(15108..15341)
                     /locus_tag="ACFNML_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6391699.1"
                     /translation="MEAIVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETETRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            15879..19313
                     /gene="dnaE"
                     /locus_tag="ACFNML_000247"
     CDS             15879..19313
                     /gene="dnaE"
                     /locus_tag="ACFNML_000247"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFC6391700.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPNAPDKPFRAMLIIRNDAGYQRLSELLT
                     EAYVGKDRNVHHAELNPEWLENGDNSGLICLSGAHYGEVGMNLLNGNEDAARAAALKY
                     AAWFPDAFYLELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMNRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPDQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVREKYGAEAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIIDVGKIPLDDQVAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMDNADQKAANANQGGL
                     FDMMEDAIEPVRLIDLPRWSESEKLAEEKTVIGFYLSGHPFGPYAQEVCQIAPTKLDR
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGSQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLS
                     SHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            19591..22865
                     /gene="cas9"
                     /locus_tag="ACFNML_000248"
                     /gene_synonym="csn1"
                     /pseudo
     CDS             19591..22865
                     /gene="cas9"
                     /locus_tag="ACFNML_000248"
                     /gene_synonym="csn1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes.;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
     gene            22931..23845
                     /gene="cas1"
                     /locus_tag="ACFNML_000249"
     CDS             22931..23845
                     /gene="cas1"
                     /locus_tag="ACFNML_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
                     /protein_id="HFC6391701.1"
                     /translation="MTWRSLLIQNGGKLSLQRRQLLIQQNGESHTVPLEDIAVIIIEN
                     RETLITAPLLSALAEHGATLLTCDEQFLPCGQWLPYAQYHRQLKILKLQLNISEPLKK
                     QFWQHIVRQKILNQAFIADETGNDLAAKRLRTLASEVRSGDTGNREAQAAALYFQALF
                     GEKFTRNDNNAVNAALNYTYAVLRAAVARTLTLYGWLPALGLFHRSELNPFNLADDFI
                     EPLRPLADLTVIHLYEQGRLKAELTPGIKQHLIKTLHYQISIERQHFSTLAAIDKMVS
                     SFQAGVTDKNAKQLKLPEILPLKEYRYE"
     gene            23838..24164
                     /gene="cas2"
                     /locus_tag="ACFNML_000250"
     CDS             23838..24164
                     /gene="cas2"
                     /locus_tag="ACFNML_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HFC6391702.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     repeat_region   24228..25253
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=24228..24263
                     /rpt_unit_seq="attgtagcactgcgaaatgagaatgggagctacaac"
BASE COUNT         6437 a         7435 c         6308 g         5447 t
ORIGIN      
        1 aaaagttacg gaacgaaaaa caaccaaaac cggacaagtc ggattcccgc ctgcgcggga
       61 atgacggaat cttaagtttc cgtctttgtt ttctgttttc tgttttcgag ggaataacgg
      121 ggaacaagcc gtatttcaga cggcattttc agttcggggt ataatccgaa tacttgcgac
      181 catctgaatc attgggacaa accatgtgtc aactgctggg catgaactgc aatacgccga
      241 ccgatattat gttttccttt gaaggcttcc gccgcagggg cggcattacc gaccaccatg
      301 ccgacggttt cggcatcggc tttttcgaag gcaaaggcgt gcgcctgttc catgacgaca
      361 aacccagtgc caattcgccc gtcgccgacc tcgtgcgcgc ctaccagatt aaatcggaaa
      421 acgtcgtcgc ccatatccgc aaagcatcac aaggacaaac ctcgctggcg aacacccatc
      481 cctttatgcg cgaaatgtgg ggcggctact ggctgtttgc ccacaacgga catttgattg
      541 atttttttcc cgaacagggc gaatttttcc accccgtcgg cacaaccgat tccgaacgcg
      601 cgttctgcca catcctcaac cgcctgcgca cccgctttgc cgcccgtccc gacgacgaca
      661 cgctgtttga cgcgattgcg gggctgacgc acgaaatccg caagttcggg ctgtttaact
      721 ttatgctttc agacggcatt gccctgtttg cccacgccag cacactactg cactacatcg
      781 tccgccaagc cccgttcggc aaggcgcgcc tgctcgacga cgacgtgatg gtcgattttg
      841 ccgaagtaac cacgcccaac gaccgcgtcg ccgttatcgc caccctgccg ctgacccgcg
      901 acgaatcatg gtcccaactt gccgtgaacg aactggtcat gttccgcgaa ggcaacatcg
      961 tccgacacga ccgtcccgaa aaccccgtct atatgagtgc cgaagaaggt ctggaaatcg
     1021 cccgcgccgc cggcgtatcg gtttgacgtt cagacggcat caggaagaaa cgctatgaaa
     1081 tgcccgtttt gcgcccaccc cgacacccgc gtcgccgatt cgcgtctgat ggaagaacgc
     1141 aacgccgtgc gccgccgccg ccactgcccc aactgcggca aacgcttcgg cacgctcgaa
     1201 accgccgaac tcaaaatgcc cgccgtcatc ggcacggaca aaaaacgctc gccctttaat
     1261 gcacaacgcc tccgcaacga cctgaccgcc gccgcccgaa aatccgccct gacacccgaa
     1321 cagatcgacg aaaccgtccg cctgacggaa cacaggctct acacttcggg tcagcgcgac
     1381 atcccctcca ccgaacttgc cgacatggtg ctaaaagagc tgcttgagca caacacggaa
     1441 gccgccgtcc gcttcgccgc cctgcacaaa cgcttcgaca atccgtcaga ctttgcctcg
     1501 tggctggcgc aagccgtcaa aacagaccgc aaagcctgat tctccccaac ccatactgat
     1561 acggtatccc tatgttttcg gacacagata tatccatgat ggaaatcgcc ctccgacttg
     1621 ccgctttggg acgtttttcc acttcgccca atccgcgcgt cggctgcgtt atcgcacacg
     1681 gcagccaaat tgtcgggcaa ggcttccacg tcaaagcggg cgaaccgcac gccgaagtcc
     1741 acgccctgcg tcaggcgggc gaaatggcac aaggcgcgac cgcctttgtt accctcgaac
     1801 cgtgcagcca ttacgggcgt accccgccct gcgccgaagc tctgctccgt tccggcgtaa
     1861 cacgcgtcgt tgccgccatg cgcgacccca acccgctggt tgccggcaaa gggcttgcca
     1921 tgctcgaagc agcaggcatc aagacggaat gcggtttact cgaacatcag gcaagggaac
     1981 tcaaccgagg cttcctgtcg cgcatcgaac gccgccgccc cttcgtccgc ctcaaatgcg
     2041 ccgtttcgct ggacggcaaa accgcccttt cagacggcag cagcttttgg attaccggcg
     2101 aagacgcgcg tgccgacgta caggttttgc gtgccgaaag ctgcgcggtg ctgaccggca
     2161 tcggcacggt gttggcggac aatccccaac tcaacgtccg cgacttccca accctgcgcc
     2221 aacccgcgcg catcgtgtta gacagccgcc tgcgcctgcc cccgtacagc catttggtta
     2281 ccgacggaca atctccgacc cttatcgcca ccttggaacg cgatgaagac aaactccgcc
     2341 cctatcggga acacgcacac atccgtatcc tgatgccgtc tgaaacggca gacagcaaaa
     2401 tcgacctgca ccacctgatg cgcctccttg ctgacgaagg tttcggcgaa atcatggtcg
     2461 aagcaggctc cgaactcaca tccgcatttt tggcagaaaa tctggcagac gaaatcgtcc
     2521 tgtaccgttc gcccaaaatc ctcggcagcg gcaaagacct gctttccctg cccgaaaacc
     2581 gcgccgccct ttccgcaccg cccttgtgga cacccgtttc caccgaaatc ctcggacacg
     2641 acatcaaaac cgtgttccgg aaaaacgata acgcctttta aagggtttgc gccgtttcac
     2701 tatataataa cgccgataaa aaacggcccc gttcagaccg ccgcccgccc ggaaaagcgc
     2761 aacccggact gccgcacccc gccggcagcc gcaacggact gaaagccgtc aaattccgat
     2821 caagaaaggc ttcagacggc acaggcagca tcccgccgcc gcccggacat caaaaatgga
     2881 cacaaaagaa atcctcggct acgcggcagg ctcgatcggc agcgcggttt tagccgtcat
     2941 catcctgccg ctgctgtcgt ggtatttccc tgccgacgac atcgggcgca tcgtgctgat
     3001 gcagacggcg gcgggactga cggtgtcggt attgtgcctc gggctggatc aggcatacgt
     3061 ccgcgaatac tatgccgccg ccgacaaaga cactttgttc aaaaccctgt tcctgccgcc
     3121 gctgctgtct gccgccgcga tagccgccct gctgctttcc cgcccgtccc tgccgtctga
     3181 aatcctgttt tcgctcgacg atgccgccgc cggcatcggg ctggtgctgt ttgaactgag
     3241 cttcctgccc atccgctttc tcttactggt tttgcgtatg gaaggacgcg cccttgcctt
     3301 ttcgtcctcg caactcgtgt ccaagctcgc catcctgctg ctgctgctga cggtcgggct
     3361 gctgcacttt ccggcgaaca ccgccgtcct gaccgccgtt tacgcgctgg caaaccttgc
     3421 cgccgccgcc tttttgctgt ttcaaaaccg atgccgtctg aaggccgtcc ggcgcgcacc
     3481 gttttcatcc gccgtcctgc atcgcggcct gcgctacggc ataccgatcg cactaagcag
     3541 catcgcctat tgggggctgg catccgccga ccgtttgttc ctgaaaaaat atgccggcct
     3601 agaacagctc ggcgtttatt cgatgggtat ttcgttcggc ggggcggcat tattgttcca
     3661 aagcatcttt tcaacggtct ggacaccgta tattttccgc gcaatcgaag aaaacgcccc
     3721 gcccgcccgt ctctcagcaa cggcagaatt cgccgccgcc ctgcttgcct ccgccctctg
     3781 cctgaccggc attttctcgc ccctcgcctc cctcctgctg ccggaaaact acgccgccgt
     3841 ccggtttatc gtcgtatcgt gtatgctgcc tccgctgttt tgcacgctgg tagaaatcag
     3901 cggcatcggt ttgaacgtcg tccgaaaaac acgcccgatc gcgctcgcca ccttgggcgc
     3961 gctggcggca aacctgctgc tgctggggct tgccgtaccg tccggcggcg cgcgcggcgc
     4021 ggcggttgcc tgtgccacct cattctggtt gttttttgcc ttcaagaccg aaagctcctg
     4081 ccgcctgtgg cagccgctca aacgcctgcc gctttatacg cacacattgc tctgcctgac
     4141 ctcctcggcg gcctacacct gcttcggcac accggcaaac taccccctgt ttgccggcgt
     4201 atgggcggca tatctggcag gctgcatcct gcgccaccgg aaagatttgc acaaactgtt
     4261 tcattatttg aaaaaacaag gtttcccatt atgaaaatcg ttttgaccac atctatggca
     4321 ggcttgggcg gcacggaaac cgccaccgtc cgcctcggca ggctactcag gcagcacgga
     4381 cacgacatca tgcttgcctc ttcggacgga ccgttgctca aagaggcgca agccttgggc
     4441 atacgttggc aacccgtcga tttctatcgc ggcggccttt tcggctacct caaaggtatg
     4501 attgcctacg cccgaatgct gcggcgcgaa caacccgaca tcatcgactg ccaaatggcg
     4561 cgcgtcgtac ccgcctgcgc cattgccgcc aaaatcgttt cgccgaaaac caaaatcatc
     4621 taccactcgc acggtttgga cgcggcaacc taccccaaaa tcgccaaact cttcgacaag
     4681 ctgggcgtgt acatcatcgg taactgcaaa cacgaacgcg aaaaactcat ccgccacggt
     4741 ttccccgccg accggattgt ctacgcctac aacgcactcc cccccccccc ggaatttcct
     4801 ttctggaaaa ccgtcaaagg ctgcgtcatg ctcggcacgc tgtcgcgtct ggacaccgtc
     4861 cgcgccgtgc atctgatgct ggacattttc aaaaaaatgg tcgaccgcga tatgcccgta
     4921 cgcctgaacg tggcgggcat aggcgaagaa atggacaatt tgaaagcgca ggcggaacgt
     4981 ctgggcatag acggcaaagt taccttcctc ggcggcgtgc gcgatttgac cgcctacttc
     5041 aaagacgtgg atattttggt gaacacgccc cactgcatcg gcgaccacgg cgcaggtgtg
     5101 ggcaacaaca tcttagaagc cggactttac gacacacccg tcgttaccta cgatatggcg
     5161 ggcatttccg aaatggtcgt caacggggaa acgggttact gcatcccctt cggcgacgaa
     5221 gaagccttta tcgccgccgt agacacgctc gtcagacaac cgctgttgcg cgaagcaatg
     5281 ggcaaaaggc tgcacgcaca cgtcgccgca ctctgttccg acgacgaaat ctaccgtacc
     5341 accatggcgg cctataccat gtaatgccgt ctgaaagccg ccgatgaaca tcaccatagt
     5401 cgccccttat tgctcgctgc cgtccgaacc gtatttcaac cgcttttggt atctggcaga
     5461 acgattgtcg caatcgcacg acgtgttgct gattaccagc cgtttccgcc actacgacaa
     5521 atcattcaga cggcatgaag atgctgccgc cacttcaaac ggcaggctgc gcgtcaagct
     5581 gctggacgaa cctggctacc gaaaaaacgt ctccctcgcc cgcgtcgcca gccaccgcgt
     5641 attcgtccgc aatttggcac gctggctgca cagcccgcag gcggcggaac aggacatcgt
     5701 ctattctgcc tatccgctga tggcgaccaa cctgctgttg ggcaaacaca aagcgcgttt
     5761 gggctacaaa ctgattgtcg atgtgcagga cgtatggccg gagtctttct cctcggtcgt
     5821 accatttttg aaaaaagtac cgcacaagct gctgcccttt gcttcacgcg ccaaccgtgc
     5881 ctaccgctgc gccgacgcgc tggttgccgt atcgcagact tatctcgacc gcgccaaaga
     5941 agccaatccg aacgtacccg gcgaagccgt ctatatcggt acggattttg ccgccatcgc
     6001 cccccccccc ccgcgattcc gctccaaaac cacccgcctt ttctacttgg gaacgctcag
     6061 ttacagctac gacgtggaaa ccgtgtgcaa aggtgttcgg aaacttttag acgacggcga
     6121 aaacgtcgaa ttgcacatta tgggcggagg acccgatttg gagaaactca aacaatatga
     6181 aaatcgcgcc atcaagtttt acggttattt accctattct gaaatgatgt cgattgccaa
     6241 aggctgcgac attgccgtca acgccatcca tagccacgcc atgcagtcgg ttaccaacaa
     6301 actctccgac tatatggctt tgcaaaaacc gattctgaac agccagacca atgccgaagt
     6361 ccttagcctc ttgaacctgc tgccgcacga aaactaccgt tccggcgacg tggacagctt
     6421 cgttcaagcc gccaaagata ttttgaaacg caaaaacgac cccgttcagt ccgatgaaat
     6481 cgtccgccgc ttcaggcgcg acatttccta tcggaaaatc gtcaacctga ttgaaagatt
     6541 ggcaaatgag taaagccgtc aaacgcctgt tcgacatcat cgcatccgca tcggggctga
     6601 ttgtcctctc acccgttttc ctgattttgg cgtacctcat ccgcaagaat ctgggttcgc
     6661 ccgtcttctt cattcaagaa cgccccggaa aggacggaaa accttttaaa atggtcaaat
     6721 tccgttccat gcgcgacgcg cttgattcag acggcattcc gctgcccgac agcgagcggc
     6781 tcacaccctt cggcaaaaaa ctgcgtgccg ctagtttgga cgaactgcct gaattatgga
     6841 atgtcttaaa aggcgacatg agcctggtcg gcccgcgccc gctgctgatg caatatctgc
     6901 cgctgtacga caacttccaa aaccgccgcc acgaaatgaa acccggcatt accggctggg
     6961 cgcaggtcaa cgggcgcaac gcgctttcgt gggacgaaaa attcgcctgc gatgtttggt
     7021 atatcgacca ttacagtttt tggctggata tgaaaatcct gtttttgaca gtcagaaaag
     7081 tctttatcaa agaagggatt tcggcggaag gggaagccac tatgccttat ttcactggaa
     7141 atcaaactaa tgaaaaaaat aatatactga ttctgtcggc aggtcgccgt gtcgaattgg
     7201 tgcaggactt ccaaaccgaa gccgcacgtt tttcagacgg catcagcgtt tttgccactg
     7261 acctcaaccc ccgcatgtct tctgcctgcc atgtttcgga cggttcattt gaagtgccgc
     7321 cgattagtac cgattcatat atcgacagca ttttcaatct ggcggtacgc gaaaatatcg
     7381 gtttaatcat cccaaccata gacaccgaac tgcaaaagct ggcagacgag cgcgaacgct
     7441 ttgaagcggc aagtgtccac atcgtcgttt ccgattcgga cttcatcgca caatgccgcg
     7501 acaaacggaa aaccgccacc ttgtttgccc gctacggcat acgctcaccc gaaatttacg
     7561 accgcaagaa gttggttttc ccttgcttcg ccaagcctta tgacggcagc cgcgccatcg
     7621 gtgcaaaacg gattgacaca cctgccgacc tgacaccgga agtcttggca gacaccaaat
     7681 taatgttctg tcagtttatc gacatcgaaa ataaattttc cgagtttacc gtcgatatgt
     7741 attacgaccg tcaatgccgt ctgaaatgcg ccatcccgcg aaaacgcttg gaagtgcgct
     7801 ccggcgaaat cagcaaaggc gttacctgca aaaacagcct ctaccaaacg ctgctggaaa
     7861 aaatggcggt attgcagggc gcgcgcggct gtatcacggc gcagttcttc tacaacgaag
     7921 aaaccggcga attctacggc gtggaaatca acccccgctt cggcggtggc ttcccgctga
     7981 cgtatgccgc aggcggcaat tatccgagcc ggttgatgcg cgaatacctc ggcgacgaag
     8041 aaatcccgtt ttcagacgac tgggaaaaca acctcattat gttgcgctac gatgccaagg
     8101 tcttggttca tgaaaacgat taatcctgaa accaccgtca tcgtcttcga cttggacgat
     8161 accctgtatt cggaatacga atacaagctc tccggtatcc gttcggtcat cgataccgtt
     8221 gccgcactgt atcccgattg gaattcagac aacctatggc gcaacatcga ccccgacggc
     8281 aaagattggc tggacaaatt gtgccgccac tgtggtttta atgaatcgga aaaacaggtt
     8341 cttttgtggc aataccggct gcaccgcccg acgctgactc cgtatgctcc gccggatttc
     8401 ctgtccgaac tgaccgcgcc cttcgccgca cgcgcgctga ttaccgacgg aagaagcctg
     8461 acccaacgcc tgaagctgga agccttgggt ctgtcttccc tgtttgacga catcctcatc
     8521 tctgaagcct gctcttcgga aaagcccgac ggcaaacgct tccgccatct tcaagacaaa
     8581 tatgccgaca aggcaggctg cttcatctat atcggcgaca acatttccaa agactttatc
     8641 gcgccgaaca ctttgggctg gataaccata ggattgcttc cctccggaca gaacatccac
     8701 cgccaccagc ctgaaagttt taacacagaa tatcatcccg acttttgggt taactccctt
     8761 caagatttag ttcctataat tgattcaaat acttaaccat tacataatca aacatgcact
     8821 cacaatatta aaaggactat ttcatgacaa aatattattc atggggcgta aacggctttc
     8881 cgccctacgt tttcgacacc atttggaaac gatggaagtt gaatgggcgc gggggcggtt
     8941 atcgtgtgcg acatcccgga cggcatgacc gtcgcgggca atccggcaaa gccgctgccg
     9001 cgcaaaaacc ccgagaccgc gacggcataa gcgattaaaa tacacccccg tacagaccga
     9061 ttttggcaac acctgcggcg cgcgcccgat tcttcggaac acgccccctt cagacggcat
     9121 agagtcagaa atgccgtctg aaaaccgacg gaccaaacac catgctgaac acctcccttt
     9181 ccccgtggcc ctgcttcacc caagaagaag ccgatgccgt ttccaaagtc ctgctgtcca
     9241 acaaagtcaa ctactggacg ggaaacgaat gccgcaaatt tgaaaaagaa ttcgccgcct
     9301 ttgccggcac gcggtacgcc gtcgcccttt ccaacggcac gctggcactc gatgtcgcgc
     9361 tcaaagcaat gggcataggc gcgggcgacg atgtgattgt tacctcgcgc actttcctgg
     9421 cttccgcatc ctgcattgtg aacgcgggcg caaatccggt gtttgccgat gtggatttga
     9481 acagccaaaa catcagcgcg gaaaccgtca aagccgtgct gaccccgaat accaaggcga
     9541 ttatcgtggt tcaccttgcc ggtatgcctg ccgaaatgga tggcattatg gctttggcaa
     9601 aagaacataa tctttgggtt gtcgaagact gcgcccaagc gcacggcgcg aaatacaaag
     9661 gcaaatccgt cggcagcatc ggacacgtcg gcgcgtggtc gttctgccaa gacaaaatca
     9721 tgaccacggg cggcgaaggc ggcatggtta ccaccaacga caaagaactg tggagcaaaa
     9781 tgtggtcgta caaagaccac ggcaaaagct atgacgccgt gtaccaccgc gaacacgccc
     9841 ctggcttccg ctggctgcac gaaagcttcg gcacaaactg gcgtatgatg gaaatgcagg
     9901 cggcaatcgg gcgcattcag ctcaaacgcc tgcccgaatg gactgtctgc cgtcaagcgc
     9961 acgccgccaa gctggcggaa agcctaggca aattcaaaag tatccgcttg gttgaagtcg
    10021 ccgactacat cggacacgcg caatataagt tctacgcctt cgtcaaaccc gaacacctca
    10081 aagacggctg gacgcgcgac cgcatcgtca acgaactgaa cgcgcgcaaa gtcccctgct
    10141 atcaaggtag ctgctccgaa gtgtatttgg agaaatcctt cgacaacacg ccttggcgtc
    10201 cgaaagagcg gctgaaaaat gctgtcgagt tgggcgacac cagcctgatg ttcttagtac
    10261 atccgacgct gaccgacggc gaaatcgcgt tctgcaaaga acacatcgaa gccgtattga
    10321 ccgaagccac acgataaccc ttcagacggc atatgccgcc tgaaagcaca taccgcccca
    10381 cgatatgact ctggaaaccc tgcttgccct accgcgcaac atcaagaaaa tctgtttcct
    10441 catacacgat tttctgatga ttttcattgc cttttggttt acccaaagcc tgaaggccga
    10501 ctactcgaac gaatggttcg accctgccaa ctggcagtct ttcctattga ccgcgccgtt
    10561 gaccatcggc ctgttcgtca gaatggggct ctaccgtgcc gttacacgct tcatcagctt
    10621 ccgcatcctc tccaccgcgc tggcaggcag cctcgcctcc gccgtattgt tcttcctcaa
    10681 cacattgatt tttgaagaaa ggctgcgcct cgccctgccg attgtctatt tcttactgct
    10741 gtttgtttcc gtgaccggct cgcgtatggt tttgcgcgga ctgttgtccg aacaccccaa
    10801 aaaacagatg atccccgtca tcatttacgg cgcggggcag gcgggcagac agcttctcga
    10861 ggccgtcaaa caaatgcgcg aatattccgc cgccgccttt gtggacgacg accccaaact
    10921 gtggcacacc gtcatctacg accttgccgt ttaccagccc gatgccatcg ccttcctcat
    10981 cgaacgctac ggcgtggaaa aaatcctact cgccatcccc ggcgcgaccc aggaacaacg
    11041 ccgccgaatc atcaacaaac tggaagccta tccgtgcgaa gtgttgacca ttcccggaat
    11101 gaaagacctg atggacggga aaatcagcat cggcacgctc aaaaaaatct ctgtgtccga
    11161 cctgctcggg cgtgattccg tcgcgcccga caaccgcctg atgagtgccg acatcgaagg
    11221 caaaaccgtc atggtaaccg gcgcgggcgg ctccatcggt tcggaactct gccgccagat
    11281 tatccgccgc cgccccgaaa agctgctgct gttcgagtta tccgaattcg ccctgtacgc
    11341 cgtcgaaaaa gaattgcgcg aaacctgcat ccaaaaacgc ctcgacaccg aaatcctgcc
    11401 ctttctcggt tcggtgcaaa accgcacgct gctcgaacac gtcatgaccg ccttttccgt
    11461 tgcgaccgtc tatcacgccg ccgcctacaa acacgtcccc atggtcgagt tcaacaccgt
    11521 cgaaggcata cgcaacaaca tcttcggcac actcgagtgc gcgcttgccg ccacgacatc
    11581 gggcgtggaa actttcgtcc tcatctccac cgacaaagcc gtccgcccca ccaacaccat
    11641 gggtgccagc aaacgcatgg cggaactctg ccttcaggcg ctcgccgccg aacccggaca
    11701 aaaaacccgc ttcagcatgg tgcgtttcgg caatgtttta ggttcgtccg gctccgtcgt
    11761 ccccgcattt gaaaaacaaa ttgccgaagg cggcccgatt accctgaccc accaagacat
    11821 tacccgctac ttcatgacca ttcccgaagc ggcgcaactg gttatccaag ccggcgcgat
    11881 gggcaaaggc ggcgacgtat tcgtcctcga tatgggcgaa tccgtcaaaa tcatcgacct
    11941 tgcccgccaa atgattacct taagcggcct caaacccaaa acacccgaac aacccgacgg
    12001 cgacatcgaa atcctcatta ccggactgcg tcccggcgaa aaactctacg aagagctgct
    12061 catcggcgac aacgtccgca aaaccggcca tccgcgcatt atgacggctg acgaggcgat
    12121 gctgccgtgg cacgagctct ctgtcctgct cgaccgcatc cgtgcggcct gcgaccgtta
    12181 cgaccagcag gcaatccgca ccctgctcat caacgccccg accggcttta ccccaagcga
    12241 cggtatctgc gacctgcttt gggtacgaga aacacacaga aaaaatgccg tctgaacctt
    12301 cagacggcat aacgtacaaa ccaacctacc ttacacacac ggagtttgac atgcagttct
    12361 cagcattcgg cgaaaaattc acgcaacaca gcggcatcct ccaactgatg gacgacctcg
    12421 gcgacgcgct caaaagcgac aagcccgtca atatgctcgg cggcggcaat ccggcgcgaa
    12481 tcgccgaagt caatgccgtg ttcgccgacg tattttccaa actggcagca gaacatgccg
    12541 tcgaaaacat cggcaactac tccaatcctc aaggagatgc cgcgctgatt gacgcgctga
    12601 ccgccttcct caaccgcgaa tacggctgga acctgaccgc cgacaatatc gcgctgacca
    12661 acggttcgca aaacgcgttt ttctatttat tcaacctctt cggcggcaaa ttcaaccttt
    12721 cagacggcac atccgcagaa aaagccattt tgttgccgct cgcgcccgaa tacatcggct
    12781 atgccgacgt gcatgtcgaa gggcagcact tcgtttccgt caagcccaaa atcgaaaacg
    12841 tcgaacacga aggcgaagca ggcttcttca aataccgcgt ggactttgac gcactggaaa
    12901 acctgcccga actcaaagcg ggcaaaatcg gcgcgatttg ctgttcgcgc ccgaccaacc
    12961 cgaccggcaa cgtgctgacc gacagcgaaa tggcgcggct ggacgctttg gcgcaagaac
    13021 acggcattcc gctgattatc gacaacgcct acggaatgcc gttccccaac atcatttaca
    13081 gcgacgtaac gctgaattgg catgaaaaca tcatcctctg cttcagcctt tccaaagtcg
    13141 gtctgccggg cgtgcgcacc ggcatcatcg tcgccgcgcc cgaagtcgtc aaagccgtca
    13201 gcagcctgaa cgcgattgtg aaccttgccc ccacgcgctt cggcgcggcc atcgccgccc
    13261 cgctactgga aagcggcgag atgaaacggc ttgccgacca agtcatccgg ccgttttacc
    13321 gcaatcaggc gcaaaccgcc gtctcgctgc tcaagcgcga gctgggcgcg tacccgatga
    13381 aaatccacaa acccgaaggc gcgattttcc tgtggctctg gtttgaaaac ctgcccgttt
    13441 cttcgcaaac cctgtacgaa atgctcaaag ccgaaggtac gctgattatt ccgggcgaac
    13501 atttcttcgt cggcatcgac acgcgggatt atccgcacgc gggcgagtgc atccgtatga
    13561 gcatcgcgca ggacgctcaa acgctggaaa aaggcattgc cgccatcggt aaaaccgtcc
    13621 gaaaactgta cgacaacgtt taaaccgcaa aaaatgccgt ctgaaggcct ttcagacggc
    13681 atttttatcc gcattcaatg tcgggaaaaa tgcgcccaca ctggcctgca gttttccggc
    13741 agttcgggac acgcgccgag gatgccgccg ctgaagtcgt ttaaacggtg gaagtcgctg
    13801 cccgcactgg cgagcatgcc gaagcgttct gccaaaaatg cgtagttgag gcggtcgttt
    13861 ttgcagcagt tgccgctgtg gacttcgatg cccgcgccgc cgaggttttt aaattcttca
    13921 aacagattgc gcttggcggt agcggacaaa tcatagcgca tcggatgggc gatgactgcc
    13981 atgccgcccg cgccgttgac ggcggagacg cagtcttcca gcgtcgccca ttcgtgtcgg
    14041 acggcgcagg atttgccgtc gcccaagtat ttggtgaacg cctgctgctt gtttttgacg
    14101 tgtcccaccc gaatgaggaa ctcggcgacg tgtgtgcggc tgaccatttc tttgtttgcc
    14161 gccagcgcga gcgcgccgtc gtatgcgccc gaaatgccgc gtttttccag cttggcggcg
    14221 atggcttcga ggcgtttcag acggccttgc cgcacttgcg ccaacaggtt ttgcaggttt
    14281 tcgtcttgtt cgtcaaagtc taagccgaca acgtgtatgg tacgcccgcg ccacgttacg
    14341 gagatttcca cgccgttaat caggcgcaaa ccgagcctgt cggcttcggc acgcgcttcg
    14401 gcgatgccgc cggtgtggtc gtggtcggtc aacgccagca gcgtgcagcc gttttgatgc
    14461 gcgaggcgca caacttcggc gggggagagc ataccgtcgg aaacggtgga atggcagtgc
    14521 aggtctatca taaggcgtta tgtgcgtggt aaatggtggg cagggtgttt caatattgtc
    14581 gggcggtgtg gcggcggtga tttcaaaaaa caggtgtatg gcaaatgcaa aggaaaagcc
    14641 gctatgccgt cattccagcg caggcgggaa tccagacttt gatttattgg aaatgtttaa
    14701 ggttaaccgc tatttcaaac ttccggattc ctgctttcga gggaatggcg atatgaccgt
    14761 tttcaatttt aatctactat aaaagactgt ctgaaaacgt ggttttatag tgaattaaat
    14821 ttaaaccggt acagcgttgg ctcgccttgc cgtactattt gtactgtctg cggctcgccg
    14881 ccttgtcctg atttttgtta attcactata tcaagccgaa ccgttttcag acggcatcgt
    14941 ccgaccaacc cgcttctttc aatttccgcc gttgcgcgtc gtagcgggcc ttatccgccc
    15001 aataaatcgt ctgaatacac gcctcgccgc agtcgctgcc gcagcattcc cacgactcgg
    15061 gtcggacggg ttcgtctaaa agcggctcgc ccaaaagggc ttcggcttta tgcttcaggg
    15121 tcatatccat cggcgatttc caagcgcgca ccgtcaaact cgatgacttc gccgccgcgt
    15181 attttggcgg ttttacgggt ttcggtttcg ccgttgcgcg acaccaaccc ttcggcaatc
    15241 aacgccttcg cctgtccgcc gctttcggca agtccggcca atttcaagag gtcgcacaag
    15301 gcgatgtatt cgttgtcttc gagatagacg atggcttcca tgatgtcccc ttgcagaaag
    15361 agaccgttat tgtagcacct gccgccgccg tacccaaaat taccgcaaaa ccagcgatgt
    15421 atccgcaccg cctgttccgt aaaagtaaaa atgccgtctg aaaccccgta tgccgcaatc
    15481 cgttcaaaga aatcctgccc aacggcagac tgcaaatcct gttccccgac gaatccgcat
    15541 tgacgctgat gcacatcctc aaacacgaac tgcccgatac accggcaatc ggcatcaaaa
    15601 cgaaatcaaa aacctgtttg aacgtaaaat taatttaact gcttgccatc cgtccgaata
    15661 aggtatatag ttccttataa ctagtttgat agtcctttat atctatcaat actccttggg
    15721 aagcctccac cacacggtag gaggcatttt tttgccgtcg taaaagctca aaaacatttg
    15781 caggtcatgc cgtctgaatc tgaaacggca tgacctacac cgccatctaa agacaaccct
    15841 gctacaatac gccttttatt gttcacgccg attttgccat gaccgagccg acctacatcc
    15901 ccctgcgcct gcataccgag ttctcaatta ccgacggtat ggtgcggatt aaaaaactga
    15961 ttgccaaagc gcaggaatac ggtttgcctg ctttgggcat cagcgatttg atgaacgaat
    16021 tcggtttggt gaaattctat aaagcctgcc gcagtgcggg gattaagcct gtcggggcgg
    16081 cggacgtgtg gataggcaat ccgaatgcgc ccgacaagcc gttccgcgcc atgttgatta
    16141 tccgcaacga tgcgggctat cagcgtttga gtgagctttt gacagaggcc tatgtcggta
    16201 aagaccgcaa tgtccatcat gcggaactca accccgaatg gctggaaaac ggcgacaaca
    16261 gcggcttgat ttgtttgagc ggcgcgcatt acggcgaagt gggcatgaat ctgttgaacg
    16321 gcaatgaaga cgcggcgcgc gcggcggcgt tgaaatatgc ggcatggttt cccgatgcgt
    16381 tttatttaga gctgcaacgc ctgcccgaac gccccgaatg ggaggcttgc gtttccggca
    16441 gcgtgaagct ggcggaggaa ctgggtttgc cggtggtggc gacgcatccg acgcagttta
    16501 tgaaccgcga cgatttcaac gcgcacgagg cgcgggtgtg tatcgcgggc ggctgggtat
    16561 tgacggacaa aaaacgtccg cgcgatttca cgccggacca gtttttcatt ccgccggaaa
    16621 cgatgctgga gcgtttcgcc gacttgcccg aagccttgga aaacacggtg gaaatcgcca
    16681 aacgctgcaa cctgcacatc acattgggca aaaacttcct gccccttttc cccacgcccg
    16741 acggtttatc gcgcgatgac tatctcatca aactctcaaa caagggcttg caggaacgca
    16801 tggttcagct ttatcccgac gaggcggagc gtgcggcaaa aatgccggaa tatcaggaac
    16861 gtttggattt tgagctgaac atcatcatcc agatgaaatt ccccggctat ttccttatcg
    16921 tacaagactt tatcaactgg gcgaaaacgc acggctgtcc ggtcgggccg ggacgcggtt
    16981 cgggcgcggg ttcgctggtg gcgtattcgc tgaaaattac cgacctcgac ccgctcaaat
    17041 acgcgctgct gttcgaacgt ttcttgaacc ctgaacgcgt ttccatgccc gacttcgacg
    17101 tggacttctg tcaggcaaac cgcggccgcg tgattgaata tgtacgcgaa aaatacggtg
    17161 cggaagcagt cagccagatt gttaccttcg gcacgatgtc gtccaaagca gtgattcgcg
    17221 acgtggggcg cgtactggaa ctgccgttta tgctgtgcga caaactgtcc aagctgattc
    17281 cgttggaagc caacaaacct ttgggtttgg acgacgcgat gaaggcggag ccgcagattc
    17341 aggaattaat cgaagcggaa gaagcggacg aactgattac gctggcgaaa aagctggaag
    17401 atttgacgcg cggtttgggt atgcacgcgg gcggcgtgtt gattgcgccg ggcaagattt
    17461 ccgattacag ccccgtgtat caggcggacg aatccgcctc gcccgtatcc atgtacgaca
    17521 agggcgacgt ggaagacgtg ggtttggtga aattcgactt tttaggtctg cgcaacctga
    17581 ccatcatcga aatggcgcag aacaacatca aaaacaccac cggcgacatc atcgatgtcg
    17641 gcaaaattcc gcttgacgac caggttgcct accaaatctt ccgcgatgcg aacaccaccg
    17701 ccgtgttcca gttcgagtcg acaggcatga agaaaatgct gaaaacagcg catacgacca
    17761 agtttgaaga acttatcgcc ttcgtatcgc tctaccgacc cggcccgatg gacaatattc
    17821 ccgacttcgt cgcgcgtatg aaaggtcagg aattccaata catccacccg ctactcgaag
    17881 gcatcctcgc accaacctac gggattatgg tgtatcagga acaagtgatg caggcggcgc
    17941 agattatcgg cggctactcg ctcggcggcg cggacctgct gcgccgcgcg atgggtaaga
    18001 aaaagcccga agaaatggtg aaacaccgcg aaatcttcgc cgaaggcgcg gcaaaacaag
    18061 gcatttcgcg cgaaaaatcc gacgaaatct tcaactatat ggaaaaattc gccggctacg
    18121 gtttcaacaa atcccacgcc gccgcctacg ccctgatttc ctaccagacc gcatggctca
    18181 aggcgcacta ccccgccgaa tttatggcgg cgaccatgtc gtccgaattg gacaacaccg
    18241 accagctcaa gcatttctac gacgactgcc gcgccaacgg catcgagttc ctgccgcccg
    18301 acatcaacga atccgactac cgctttacgc cgtatccgga catgaaaatc cgctacgcgc
    18361 tcggcgcgat taaaggcacg ggcgaagccg ccgtcgaatc catcaccgcc gcgcggcaaa
    18421 gcggcggcaa gtttaccggc ctgttggact tctgcgagcg cgtcggcaaa gaacacatga
    18481 accgccgcac cctcgaagcc ctgatacgcg gcggcgcgtt cgacagtatc gaacccaacc
    18541 gcgccatgct cttggcgaac atcgacctcg ctatggacaa cgccgaccaa aaagccgcca
    18601 acgccaatca gggcgggctg ttcgacatga tggaagacgc catcgaaccg gtgcggctca
    18661 tcgacctgcc gaggtggagc gaatcggaaa aactcgccga agaaaaaacc gtcatcggct
    18721 tttacctgtc cggccacccg ttcggcccgt atgcccaaga agtctgccaa atcgcaccga
    18781 ccaaattaga ccgtctgaag ccgcaagaca gcgtgcgcct cgccggattc gttaccgccg
    18841 tgcgtacgat gatgggcaag cgcggcaaaa tcgccttcgt cagccttgaa gatttgagcg
    18901 ggcaggttga aatcatggtc ggcagtcaga cgctggaaaa ctgcgccgac tgcctcaaag
    18961 ccgaccaagt gctgattatc gaatccaaag tcagccgcga cgactacggc ggcggcgacg
    19021 gactgcgcat catggcaaac caagtcatga ccctgcaaac ggcacgcgaa cgctacgccc
    19081 gcagcctcag cctcgccctc gccccgcatc acgacatcgg cggactggta cggctgctct
    19141 cctcccacca actgcccgac acgccgcgca tcccgctgca actgtcgtat gccaacgaaa
    19201 aagcgtcggg caggcttcaa gtgccgccga aatggacggt tacaccgagc tccgcattgt
    19261 tcggcgaact ggaaacattg ctcggcagcc ggtcggtgcg cgtcaactgg taacccaaaa
    19321 tataaatgcc gtctgaagcc caaaatcggt ttcagacggc attttttaac cgaaataaac
    19381 gatgcccctt aaagcagaag ctttaagggg cagagcgttg cggcacatct tttcagacgg
    19441 ccttattgta gcaacggttc tcatttcgca gtgcgacaat atgtttggga ttctagccgt
    19501 tgtgagatga taagtcaata atctatttgt atataaataa gacaataaga tatattatca
    19561 ttatttgcaa cgttgtttac ggagtaataa atggctgcct tcaaacctaa tccaatcaac
    19621 tacatcctcg gcctcgatat cggcatcgca tccgtcggct gggcgatggt agaaattgac
    19681 gaagaagaaa accccatccg cctgattgat ttgggcgtgc gcgtatttga gcgtgccgaa
    19741 gtaccgaaaa caggcgactc ccttgccatg gcaaggcgtt tggcgcgcag tgtccgccgc
    19801 ctgacccgcc gtcgcgccca tcgcctgctt cgggcccgcc gcctattgaa acgcgaaggc
    19861 gtattacaag ccgctgattt tgacgaaaac ggcttgatca aatccttacc gaatacacca
    19921 tggcaacttc gcgcagccgc attagaccgc aaactgacgc ctttagagtg gtcggcagtc
    19981 ttgttgcatt taatcaaaca ccgcggttat ttgtcgcaaa gaaaaaacga gggcgaaact
    20041 gccgataaag agcttggcgc tttgcttaaa ggcgtggcca acaatgccca tgccttacag
    20101 acaggcgatt tccgcacacc ggccgaattg gctttaaata aatttgagaa agaaagcggc
    20161 catatccgca atcagcgcgg cgattattcg catacgttca gccgcaaaga tttacaggcg
    20221 gagctgattt tgctgtttga aaaacaaaaa gaatttggca atccgcatgt ttcaggcggc
    20281 cttaaagaag gtattgaaac cctactgatg acgcaacgcc ctgccctgtc cggcgatgcc
    20341 gttcaaaaaa tgttggggca ttgcaccttc gaaccggcag agccgaaagc cgctaaaaac
    20401 acctacacag ccgaacgttt catctggctg accaagctga acaacctgcg tattttagag
    20461 caaggcagcg agcggccatt gaccgatacc gaacgcgcca cgcttatgga cgagccatac
    20521 agaaaatcca aactgactta cgcacaagcc cgtaagctgc tgggtttaga agataccgcc
    20581 tttttcaaag gcttgcgcta tggtaaagac aatgccgaag cctcaacatt gatggaaatg
    20641 aaggcctacc atgccatcag ccgtgcactg gaaaaagaag gattgaaaga taaaaaatcc
    20701 ccattaaacc tttcttccga attacaagat gaaatcggca cggcattctc cctgttcaaa
    20761 accgatgaag acattacagg ccgtctgaaa gaccgtgttc agcccgaaat cttagaagcg
    20821 ctgttgaaac acatcagctt cgataagttc gtccaaattt ccttgaaagc attgcgccga
    20881 attgtgcctc taatggagca aggcaaacgt tacgatgaag cctgcgccga aatctacgga
    20941 gaccattacg gcaagaaaaa tacggagacc attacggcaa gaaaaatacg gaagaaaaaa
    21001 tttatctgcc gcccatccct gccgacaaga tccgcaaccc cgtcgtcttg cgcgccttat
    21061 ctcaagcacg taaggtcatt aacggcgtgg tacgccgtta cggctcccca gctcgtatcc
    21121 atattgaaac ggcaagggaa gtaggtaaat cgtttaaaga ccgcaaagaa atcgaaaaac
    21181 gccaagaaga aaaccgcaaa gaccgggaaa aagccgccgc caaattccga gagtatttcc
    21241 ccaattttgt cggcgaaccc aaatcaaaag atattctgaa actgcgcctg tacgagcaac
    21301 aacacggcaa atgcctgtat tcgggcaaag aaatcaactt agtccgtctg aacgaaaaag
    21361 gctatgtcga aatcgaccat gccctgccgt tttcgcgcac atgggacgac agtttcaaca
    21421 ataaagtgct ggtattgggc agcgaaaacc aaaacaaagg caatcaaacc ccttacgaat
    21481 acttcaacgg caaagacaac agccgcgaat ggcaggaatt taaagcgcgt gtcgaaacca
    21541 gccgtttccc gcgcagtaaa aaacaacgga ttctgctgca aaaattcgat gaagacggct
    21601 ttaaagaatg caatctgaac gacacgcgct acgtcaaccg tttcctgtgc caatttgttg
    21661 ccgaccatat attgctgaca ggtaaaggga aaagacgtgt ctttgcctca aacggacaaa
    21721 ttaccaatct gttgcgcggc ttttggggat tgcgcaaagt gcgtgcggaa aacgaccgcc
    21781 atcacgcctt ggacgctgta gtcgttgcct gctcgaccgt tgccatgcag cagaaaatta
    21841 cccgttttgt acgctataaa gagatgaacg cgtttgacgg taaaaccata gacaaagaaa
    21901 caggaaaagt gctgcatcaa aaaacacact tcccacaacc ttgggaattt ttcgcacaag
    21961 aagtcatgat tcgcgtcttc ggcaaaccgg acggcaaacc cgaattcgaa gaagccgata
    22021 ccccagaaaa actgcgcacg ttgcttgccg aaaaattatc atctcgcccc gaagccgtac
    22081 acgaatacgt tacgccactg tttgtttcac gcgcgcccaa tcggaagatg agcggtgcac
    22141 ataaagatac tttgagatct gctaaacgat ttgttaaaca taatgaaaaa attagtgtta
    22201 aacgagtatg gttaaccgaa atcaagttgg ccgaccttga aaatatggtt aattataaaa
    22261 atggtagaga gattgaatta tatgaggctc ttaaggcgcg tttagaggca tatggaggta
    22321 atgctaaaca agcatttgac cctaaggaca atccgtttta taaaaaggga ggacaactgg
    22381 ttaaagctgt gagggtcgaa aaaacccaag agagcggagt cttattaaat aaaaaaaatg
    22441 cttataccat tgcagataat ggagacatgg tacgtgttga tgtgttctgt aaagtagata
    22501 agaaaggaaa aaatcagtat tttattgttc ccatctatgc ttggcaggtt gctgaaaaca
    22561 ttcttcccga tattgattgt aagggatacc ggattgatga tagctataca ttctgtttta
    22621 gcttgcataa gtatgatctg attgcttttc aaaaagatga aaaatctaaa gtagaattcg
    22681 cctactatat caactgtgat agctctaatg gacgattcta tttagcttgg catgataaag
    22741 gctctaaaga acagcaattc cgtattagca cccaaaatct tgtattgata caaaaatacc
    22801 aagttaacga actgggcaaa gaaatcagac catgccgtct gaaaaaacgc ccacctgtcc
    22861 gttaaattaa tctgtccctg cttcagacgg cctgaagcag ggatttttta tatcaaaata
    22921 aggggaaaac atgacttggc gcagcctact tattcaaaac ggcggcaaac tatccttgca
    22981 acgccggcaa ctgctgattc aacaaaacgg cgagtcccat accgtaccat tagaagacat
    23041 cgccgttatc atcatcgaaa atcgcgaaac cctgattacc gccccgctcc tatcagcctt
    23101 agctgaacat ggcgcaaccc tgctgacctg cgacgagcag ttcctgccct gcggacaatg
    23161 gctgccttat gcccaatatc accgccagct taaaatcctc aagctgcaac tgaacataag
    23221 cgaaccctta aaaaagcaat tctggcaaca tatcgtccgc caaaaaatcc tgaaccaagc
    23281 atttatcgca gacgaaacag gcaatgatct tgcagccaaa cggctgcgca ctttggcatc
    23341 tgaagtccgt tcgggcgata caggaaaccg tgaagcccaa gctgccgccc tctacttcca
    23401 agccctcttt ggcgaaaaat ttacccgtaa tgacaacaat gccgtcaatg ccgcattgaa
    23461 ctacacctac gccgtgctac gggccgcagt tgcacgcacc ctgaccttat acggctggct
    23521 gcctgcatta ggcttgtttc atagaagcga actcaatcca ttcaatctcg ccgatgactt
    23581 tatcgaaccc ttacgcccat tagccgacct gaccgtgata catttgtacg aacaaggccg
    23641 tctgaaagcc gaactgaccc ccggcatcaa acaacacctt atcaaaaccc ttcactatca
    23701 aatcagcatc gaacggcaac atttcagcac cttggctgcc atcgacaaaa tggtttcgtc
    23761 atttcaagcc ggtgtaaccg ataaaaacgc caaacaactg aaactgcctg aaatcctacc
    23821 attgaaagaa taccgatatg agtgaggcca aatttatgag gattatcgtc ttcttcgacc
    23881 taccggttat cacggcggca aagcgcaaag ccgccaatca attccgccag tttttattaa
    23941 aagacggata ccaaatgctc cagctttccg tatacagccg tatcgtcaaa ggccgcgatt
    24001 cgttgcaaaa acaccacaat agactatgtg caaaccttcc gcaagaaggc tcaatccgct
    24061 gtttggagat aacagaaaag caatatgccg ccatgaaact gctgttgggc gagctgaaaa
    24121 cccaagaaaa aaaggtcaat tcagaccaat tattgttatt ttaagcccat tttttcagac
    24181 taaataaaac gggaaatcct tatagaataa ggatttcccg ttggggtatt gtagcactgc
    24241 gaaatgagaa tgggagctac aacaaaagtt tatgacttgt acgaatccgc agaattgtag
    24301 cactgcgaaa tgagaatggg agctacaacc cctgattgca ggtgaagatg aactgtatga
    24361 ttgtagcact gcgaaatgag aatgggagct acaacaaaat cgtcggcctg ctccagcacg
    24421 acctgattgt agcactgcga aatgagaatg ggagctacaa cctacccgct cggcgtatat
    24481 gaggcaatct aattgtagca ctgcgaaatg agaatgggag ctacaacgcg cacgattgac
    24541 ggcagcagac gagccaaatt gtagcactgc gaaatgagaa tgggagctac aacagcggtg
    24601 gtagaatcgg atttttagag tgaattgtag cactgcgaaa tgagaatggg agctacaacc
    24661 tcgaatggcg agtacttgac gctcaataca ttgtagcact gcgaaatgag aatgggagct
    24721 acaaccaggt gcttgggcgt aattttatcc gttggattgt agcactgcga aatgagaatg
    24781 ggagctacaa cccttttgca acaatcgtgc tttatttgtc aattgtagca ctgcgaaatg
    24841 agaatgggag ctacaacttc ctgtcggctt tttcggctgc tttgcggatt gtagcactgc
    24901 gaaatgagaa tgggagctac aactatgctt gggtttgagt gggcgattga tgaattgtag
    24961 cactgcgaaa tgagaatggg agctacaacc ttggcacgag atggtcgccg tattaaatta
    25021 ttgtagcact gcgaaatgag aatgggagct acaactttat ggctattcac ctgtcgagca
    25081 aatcaattgt agcactgcga aatgagaatg ggagctacaa caagaagatt gggaaatgta
    25141 tctcacggaa gattgtagca ctgcgaaatg agaatgggag ctacaaccgg tttgaacagc
    25201 agtatatggg aagaaacatt gtagcactgc gaaatgagaa tgggcgctac aaccagccgt
    25261 tgcgataagc gaacgtgcgt taaagtcatt gagacacagt gcaattatgg caagaaaggc
    25321 gacagatatt gtgtcacaga tttgaaataa gaaaaaaccg cccactcaca gagtaggcgg
    25381 aaaacttgcg tttttttgat tgtggtcggg ttagctactg cattgactta acagcaaagc
    25441 aaccgctaaa atgaacttgt ggttcacttc aacggacagc agcaactttt tcccacgcat
    25501 aaggaattta agcattttag cttctcctag tgaagttaat cacacaggcg agagccgaac
    25561 aaaaatcccc gttgcagcgg ggatttttgc tgttccctgt ttgatgatta aactaaaatc
    25621 ttgcaaa
//
LOCUS       DAXHTR010000008        24006 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.008, whole genome shotgun sequence.
ACCESSION   DAXHTR010000008 DAXHTR010000000
VERSION     DAXHTR010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24006
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.008"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(91..1977)
                     /gene="uvrC"
                     /locus_tag="ACFNML_000251"
     CDS             complement(91..1977)
                     /gene="uvrC"
                     /locus_tag="ACFNML_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFC6391703.1"
                     /translation="MPSESVFQMIDMNTENRSPEQFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVRHIETTITRSEAEALILENNFI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYHDSVREAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGLVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IISNFSVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDAVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPNSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLSDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2031..4208)
                     /locus_tag="ACFNML_000252"
     CDS             complement(2031..4208)
                     /locus_tag="ACFNML_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6391704.1"
                     /translation="MQQKIRFQIEGMTCQACASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MVGMMLKGLNWTRHDWMLSPLLQFALASVVQLWLAVPFYKSAWASIKGGLANMDVLVT
                     IGTVSIYLYSIYMLFFSPHAAYGMAHVYFEAGVMVIGFVSLGKFLEHRTKKSSLNSLG
                     LLLKLTPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHL
                     TGESNPEEKKAGSKVWAGALMTEGSVVYRATQLGSQTLLGDMMNALSEAQGSKAPIAR
                     VADKAAAVFVPTVVGIALLTFIVTWLIKGDWTLALMHAVAVLVIACPCALGLATPAAI
                     MVGMGKAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDGGFDED
                     ALYRIAAAVEQNAAHPLARAIVSAAQARGLGIPAAQNAQTVVGAGIAAEVESVGLVKA
                     GKAEFAELTLPKFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNI
                     DVYIMSGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDA
                     PALAAANVSFAMKGGADVAEHTASATLMQHSVNQLADALSVSRATLKNIKQNLFFAFF
                     YNILGIPLAALGFLNPVIAGAAMAASSVSVLGNALRLKRVKIY"
     gene            4347..5297
                     /gene="trxB"
                     /locus_tag="ACFNML_000253"
     CDS             4347..5297
                     /gene="trxB"
                     /locus_tag="ACFNML_000253"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFC6391705.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5453..5821
                     /gene="rpsF"
                     /locus_tag="ACFNML_000254"
     CDS             5453..5821
                     /gene="rpsF"
                     /locus_tag="ACFNML_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFC6391706.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5822..6124
                     /gene="priB"
                     /locus_tag="ACFNML_000255"
     CDS             5822..6124
                     /gene="priB"
                     /locus_tag="ACFNML_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFC6391707.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGIPVLDIILKHKSWQEENGQ
                     QCLVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIQEYKG"
     gene            6131..6361
                     /gene="rpsR"
                     /locus_tag="ACFNML_000256"
     CDS             6131..6361
                     /gene="rpsR"
                     /locus_tag="ACFNML_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFC6391708.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6378..6830
                     /gene="rplI"
                     /locus_tag="ACFNML_000257"
     CDS             6378..6830
                     /gene="rplI"
                     /locus_tag="ACFNML_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFC6391709.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARVRQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            complement(7797..8603)
                     /locus_tag="ACFNML_000258"
     CDS             complement(7797..8603)
                     /locus_tag="ACFNML_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6391710.1"
                     /translation="MWHWDIILILLAVGSAAGFIAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFTGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTDPQTASRPLPGLPGLTAVSTLFGTMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8604..9350)
                     /gene="pssA"
                     /locus_tag="ACFNML_000259"
     CDS             complement(8604..9350)
                     /gene="pssA"
                     /locus_tag="ACFNML_000259"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFC6391711.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(9424..9549)
                     /locus_tag="ACFNML_000260"
     CDS             complement(9424..9549)
                     /locus_tag="ACFNML_000260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391712.1"
                     /translation="MPSNNIHPAKIRNNTPGGRQSQTAPQSIRHQKNKPPPRASS"
     gene            9933..10415
                     /locus_tag="ACFNML_000261"
     CDS             9933..10415
                     /locus_tag="ACFNML_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HFC6391713.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGVMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(10467..11678)
                     /locus_tag="ACFNML_000262"
     CDS             complement(10467..11678)
                     /locus_tag="ACFNML_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease family protein"
                     /protein_id="HFC6391714.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQVNWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLTGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11901..14339
                     /locus_tag="ACFNML_000263"
     CDS             11901..14339
                     /locus_tag="ACFNML_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6391715.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVMLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAGAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDNPEPLEKLPMIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNVDSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPTD
                     LNGSRKILAHKDHL"
     gene            14550..15860
                     /gene="tig"
                     /locus_tag="ACFNML_000264"
     CDS             14550..15860
                     /gene="tig"
                     /locus_tag="ACFNML_000264"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFC6391716.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKAPLKMIAQMYGASAQNDVINEMVQRRFYDVAVAQELKVAGYPRFEGVEEQDDKE
                     SLKIAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRAIIDFEGKIDGEPFAGGASKNYAFVLGAGQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YAEPSRLQAPTSLAIESNVVDFVLGKAKVNEKALSFDEVMGAQV"
     gene            15956..16570
                     /gene="clpP"
                     /locus_tag="ACFNML_000265"
     CDS             15956..16570
                     /gene="clpP"
                     /locus_tag="ACFNML_000265"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFC6391717.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(16627..17892)
                     /gene="ispG"
                     /locus_tag="ACFNML_000266"
                     /gene_synonym="gcpE"
     CDS             complement(16627..17892)
                     /gene="ispG"
                     /locus_tag="ACFNML_000266"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFC6391718.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSVWRTAYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(17908..18669)
                     /gene="pilW"
                     /locus_tag="ACFNML_000267"
     CDS             complement(17908..18669)
                     /gene="pilW"
                     /locus_tag="ACFNML_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HFC6391719.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQKSFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18672..19766)
                     /gene="rlmN"
                     /locus_tag="ACFNML_000268"
     CDS             complement(18672..19766)
                     /gene="rlmN"
                     /locus_tag="ACFNML_000268"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HFC6391720.1"
                     /translation="MKTNLLNYDLQGLTRHFADMGEKPFRAKQVMRWMHQSGAQNFDE
                     MTDLAKSLRHKLNEQAGIEIPKLMMSQKSSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDKAQHAR
                     ELIELVTDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19910..20335)
                     /gene="ndk"
                     /locus_tag="ACFNML_000269"
     CDS             complement(19910..20335)
                     /gene="ndk"
                     /locus_tag="ACFNML_000269"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFC6391721.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPAEAAEGT
                     IRADFATSVSINAVHGSDTPENAALEIAYFFSQTEICPR"
     gene            complement(20394..21545)
                     /gene="zapE"
                     /locus_tag="ACFNML_000270"
     CDS             complement(20394..21545)
                     /gene="zapE"
                     /locus_tag="ACFNML_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFC6391722.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKDSGG"
     gene            complement(21936..22763)
                     /gene="fghA"
                     /locus_tag="ACFNML_000271"
     CDS             complement(21936..22763)
                     /gene="fghA"
                     /locus_tag="ACFNML_000271"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFC6391723.1"
                     /translation="MKLIEQHQIFGGLQQVWAHDAQTLQCEMKFAVYLPNNSENQPLG
                     VIYWLSGLTCTEQNFITKSGFQLYAAEHQVIVVAPDTSPRGEQVPNDAAYDLGQGAGF
                     YLNATEQPWATNYQMYDYILNELPDLVEANFSTNGKRSIMGHSMGGHGALVLALRNRK
                     RYQSVSAFSPILSPSLVPWGEKAFTAYLGEDREKWQQYDASSLIQQGYKVQGIRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(22772..23881)
                     /locus_tag="ACFNML_000272"
     CDS             complement(22772..23881)
                     /locus_tag="ACFNML_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase [NAD(P)+] activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HFC6391724.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPSKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFDLMHEGKSIRAVIHY"
BASE COUNT         5822 a         5949 c         6348 g         5887 t
ORIGIN      
        1 cgtactggtt taaatttaat ccactatata tcggtatacc gacctgctgt atcatatttt
       61 ttacagacgg attttgtctt tgacagcatg ctaatgcaga tgattgtaaa tcgtttccgc
      121 caatgccttg ctgatgcctt ccactttttc caagtcctcg cggctggcgg caatcacgcc
      181 gcgcagaccg ccgaagcggg tgagcagggc ttggcggcgt ttgctgccta cgccggggat
      241 gtcgcttaag gaggaggtaa cgcgggcttt gtcgcgtttt ttgcggtgac cggtaatggc
      301 gaaacggtgc gattcatcgc gtacggtttg caatagatgc aaggccgggc tgttgggcgg
      361 caggcggaag acttcgccgg taaaaggcag tatgagctcc tccataccgg ctttgcgctc
      421 cgggcctttg gcaatgccga ccaaagggat gtgcagcccg agttcttccc ataccgatac
      481 ggctacgccg atttgccctt tgccgccgtc aatcaacacg gcatccggcc atttgacggt
      541 ctcgccgttg gcttcggcct cctgcatttt gccgtaacgg cgcgtcaaca cttcgcgcat
      601 ggcggcgtag tcgtcgccgg gtttggcggt cgtgatgttg tagcggcggt attgcgaagg
      661 ctggatgttt tgctcatcgt acacaacgca ggacgcaata gtggcttcgc cttgtgtgtg
      721 gctgatgtcg aaacattcaa ggcggttgag gccgtctgaa tccatgccga ggatttttgc
      781 cagttcatca atgcggtgtt gctggctgct ttgttgcagg cggcgttgtg caatcgccat
      841 ttgtgcgttt tgttccgcca ttttcaacca gactttgcgt tcgcctatgg ttttggtgac
      901 aaattgcatc tgcttgccgt gttcgccttc caaagcctct ttcagcgcat cgggaacgga
      961 aaagttgcta ataatgatgt cgggtttgct tttgcccaaa taatgttggg cgacgaaggc
     1021 ttcggcgtaa tcttgtccgt ttggctcggg atcgtttttg gtgtcgggga aaaagctttt
     1081 atcgcctaca tgccgtccgc cgcgtatgct gacccagtgt acgcagacga ggccgtctga
     1141 aacggcgagc gcgagcaggt cgatgtcgtt ggggttgttc gggtttttgc tgtcgatgaa
     1201 ctggttgctc tgcataatgc cgagtgcttg gatttggtcg cggtagcggg cggcttcttc
     1261 aaattgcaga ttagcggcgg cggtttgcat tttgtgttgc agggtacgcg tcagttcgtc
     1321 ggttttgcca ttgaggaaag ttgctgcttc acgcacgctg tcgtgataat cttcttcgct
     1381 gatatgaccg acacaaggtg cggtacagcg tttgatttgg taaagcagac aaggacggtc
     1441 gcggtgctcg aatacactgt cttcgcaggt acgcagcata aagacttttt gcaacacttg
     1501 aatactgtcg cgcacggcgt tgctgttggg atacgggccg aaatattgat ttggcttttt
     1561 cagtgtgccg cggtaatacg ccatttgcgg atattgatgg ccactgagca tcaaataagg
     1621 atagcttttg tcgtcgcgga aaaggatatt gtatttcggc gacaaggctt tgatgaagtt
     1681 gttttcgaga atcagggctt cggcttcgga acgcgtgatg gtggtttcga tgtggcgaac
     1741 ctgttttacc atcaatgcga tgcgcggcga atggtcgttt ttttggaaat agccggacac
     1801 gcgccgcttg aggttgaccg ctttgccgac gtataagact ttgccgcctt cgtcaaaaaa
     1861 acggtacacg cccggcagct tgggcaggtt tttgaggaag agcgggatgt cgaattgttc
     1921 tggagaacgg ttttctgtat tcatatcaat catttgaaaa acggattcag acggcatatc
     1981 gacaacggca gccttatccg ttcggaaagg ctgcaggctg ttatatgcgg tcaatagatt
     2041 tttacccgtt tcaggcgcaa ggcattgccc aataccgaaa ctgagcttgc cgccattgcc
     2101 gcgcctgcga tgacgggatt taaaaagccg agtgcggcaa gtggaatgcc caaaatattg
     2161 tagaagaagg cgaaaaacag gttttgcttg atgtttttca acgtcgcccg cgataccgac
     2221 agcgcatcgg cgagctggtt gaccgaatgc tgcatcagcg tggcggacgc ggtgtgttcg
     2281 gcaacgtccg cgccgccttt catcgcaaag ctgacgttgg cggcggcgag cgcgggcgcg
     2341 tcgttgatgc cgtcgccgac catcgccacg gttttgccgg cggctttgag tttctgtact
     2401 tcggcagctt tgtcgcgcgg actcatatta ccgaaggcgt gtgcgatgcc cagttgtttg
     2461 gcgacgtact cgaccgtgcc ttggttatcg ccgctcataa tatagacatc gatattgtgt
     2521 tttttcagac gacctatggc ttcggcggta tcggctttca acgcgtcggc aagtgcgaat
     2581 gcgccgatgg gtttgttatc gactgagacc gcaacaatgc ttgcaatgtc ccaaacgccg
     2641 tctgaaaact tcggcaaggt cagttcggca aattcggctt tgcccgcttt caccaacccc
     2701 acgctttcca cttcggcggc aatgcctgcg ccgacaacgg tttgcgcgtt ttgtgcggcg
     2761 ggaatcccca agccgcgcgc ttgggcggcg gagacgatgg cacgggcgag cggatgggcg
     2821 gcgttttgtt cgacggcggc ggcgatgcgg tacaaagcgt cttcgtcaaa gccgccgtcg
     2881 ggaacacaat aaacggcggc aacctgcggc ctgccttcgg tcagcgtacc ggttttgtcc
     2941 aacacgacgg catcgacgtg ggcggcttct tccattgccg ccgcatcttt aaaccaaata
     3001 ccgtgtttca ctgctttgcc catgccgacc ataatcgcgg cgggcgtcgc caaaccgagc
     3061 gcgcacgggc aggcaatcac caaaacggcg acggcgtgca tcagtgcgag cgtccaatcg
     3121 cccttaatca gccaagtaac aataaaagtc aacaacgcaa tgcccacgac agtcggcacg
     3181 aacaccgccg ccgccttgtc cgccacgcgc gcaatcggtg ctttgctgcc ttgcgcttca
     3241 gagagcgcgt tcatcatgtc gccgagcagg gtttggctgc cgagctgcgt ggcgcgatac
     3301 accacgctgc cttcagtcat cagcgcgccc gcccacactt tgctgcctgc ttttttctct
     3361 tcgggattgg attcaccggt aagatggctc tcatccgccc aaccgctgcc gctttcaata
     3421 atgccgtcgg cggcaatgcg ttcgccgtgg ttggcgcgga ttaggtcgcc gatttgcact
     3481 tggtcgatgg gcagttgttt ccattcgcca ttgcgttgta cattgacttg ggtcggcgtg
     3541 agtttgagca gcaggcccaa gctgttcagg ctggattttt tggtgcggtg ttccaaaaat
     3601 ttgcccagcg acacaaaacc aatcaccatc acgcctgctt caaaatacac atgcgccata
     3661 ccgtacgccg cgtgcgggct gaaaaacagc atatagatgg aatacagata aatcgacacc
     3721 gtgccgatgg taacgagtac gtccatattc gccagcccgc ctttaatgct cgcccacgcg
     3781 cttttgtaaa atggcaccgc cagccaaagc tgcaccacac tcgccaatgc aaactgcaac
     3841 aagggcgaca acatccaatc atgccgtgtc caattcagcc cttttagcat catccctacc
     3901 ataccgataa ggaaggggat attgatggcc agcaaaagcc acaacctcca gccgatatgg
     3961 tgttctgctt cgggttgcgg caatgtatct tccgtctttt ccttcgcgcc gtaaccggtt
     4021 ttctcaatga ttttggcaat gtcggcagcg gaagttttgc tgtcgtcaaa cgtaacctgc
     4081 gcctcctcgc tggcgaagtt cacccccgcc gattcgacaa agtctttttt gttcaacact
     4141 ttttcaatgc gcgaagcgca ggcctggcag gtcatgcctt cgatttggaa acggattttt
     4201 tgttgcataa gcgtgattcc tgctttgata ttcagacggc agttaaggct gcgggatgcc
     4261 gtctgagcgc cggtatggtc aaaatttctc aaacgcgccg ataattcgag tagaatccga
     4321 aaacttgaca cagcaaagga aaaacaatgt cccaacaccg caaactgatt attttgggtt
     4381 ccggccccgc cggatacacc gccgccgtct atgccgcgcg cgccaattta aaccccgtca
     4441 ttattacagg tatcgcgcaa ggcgggcaac tgatgaccac caccgaagtg gacaactggc
     4501 ctgccgatgc cgacggcgtg caagggccgg aattgatggc gcggtttctc gcccatgccg
     4561 aacgtttcgg aacggaaatc atttttgacc aaatcaacgc cgtcgatctg caaaaacgcc
     4621 cgtttacact caaaggcgat atgggcgagt acacttgcga tgccctgatt gtcgcaacag
     4681 gcgcgtccgc caaatacctc ggtttgccga gtgaggaagc gtttgcagga aaaggcgttt
     4741 ccgcttgtgc cacctgcgac ggtttcttct acaaaaacca agatgttgcc gtagttggcg
     4801 gcggcaatac ggcagttgaa gaggcactct accttgccaa tatcgccaaa accgttaccc
     4861 tgatccaccg ccgcagcgag ttccgtgccg aaaaaatcat gatcgacaaa ctgatgaaac
     4921 gcgtggaaga gggcaaaatc atcctcaagc tggaaagcaa cctgcaagaa gtactgggcg
     4981 acgatcgggg cgtaaacggc gcattattaa aaaacaacga cggttctgag caacaaattg
     5041 ccgtcagcgg catttttatc gccatcgggc acaagccgaa taccgatatt ttcaaaggtc
     5101 agttggaaat ggacgaagcc ggttacctga aaaccaaagg cggcacggcg gacaatgtcg
     5161 gcgcaaccaa tatcgaaggc gtatgggcgg cgggcgacgt aaaagaccat acctaccgtc
     5221 aggcgattac cagcgcggct tccggctgtc aggcggcttt ggacgcggaa cgctggctgg
     5281 gcagccaaaa catttgaatg ccgtctgaaa ccgaaatttt cggcggaatg caaatttttc
     5341 gcaccaaatc cttgattccg tcggcaaacc ccaatataat gcctgctctt tgaaccttgc
     5401 tttctgcttt cgcatggcag aaggctgttt ttaccatcca taaggagata acatgcgtca
     5461 ttacgagatc gtgtttatcg ttcatcctga tcaaagcgag caagtgcctg ctatggttga
     5521 acgttacaaa accatgattg ccgaagcaaa cggtaagatt caccgtttgg aagattgggg
     5581 tcgccgccaa ctggcttacc cgattaacaa aatccataaa gcccattacg ttttgatgaa
     5641 catcgaaacc actcccgaag tggttgaaga gctggaaacc gcattccgct tcaatgatgc
     5701 cgtattgcgc catctaacca tcaaaaccaa acacgccgtt accgaagcat cccctatgtt
     5761 gggtggtgaa aaggctaaga acctgttgag cggtgcgtct gaagaagcgg tcgcccaata
     5821 attgggattc aataatcttg tttcgcttgc cgcgttaatt gaaaaggttt tccctattcg
     5881 atatacgcct gccggaatcc ctgttttaga tattatttta aagcacaaat cgtggcagga
     5941 ggaaaacggg cagcaatgcc ttgtccaatt ggaaattccg gcacggattt taggcaggca
     6001 ggcggaagag tggcagtatc ggcaaggtgt atatgttcac gtcgaaggtt ttttagctca
     6061 aaaaagcaga cgttccctta tgccgatgct caggatacaa aatattcaag aatataaagg
     6121 ttaaacgaca atggctcgtc aatcattcaa acgtagaaaa ttctgccgtt tcacggctga
     6181 aaaaatccaa gaagtcgatt acaaacaagt tgatttgctg aaagacttta tctctgaaaa
     6241 cggtaaaatc attcctgcac gcatcacagg aacgaaggca ttctaccaac gccaattggc
     6301 tgttgccgta aaacgcgcac gcttcctggc tctgctgcct tacaccgacc aacacaaata
     6361 attttggaga ttgaatcatg caaattattc tgttagaaaa aatcggcggt ctgggcaact
     6421 tgggcgacat cgtaaccgtt aaaaacggct acgcccgcaa ctttctaatt cccgcaggta
     6481 aggcaaaacg tgcgaccgaa gcgaatatga aagagtttga agcacgccgt gccgaattgg
     6541 aagccaaaca ggctgaaatt ttggcagatg cccgagtccg tcaggaaaaa ttggacggtc
     6601 aaaccgttac cgttgctcaa aaagccggtg tggacggccg cctgttcggt tccgttacca
     6661 atgccgacat tgctgctgca atcgttgctg ccggcatcga agccgtgaaa gcaaatgtac
     6721 gtctgccgaa cggtcctctg aaagccgttg gcgagtacga agtggaagtg gctttgcaca
     6781 ctgatgccgt tgctaaaatt accgttgccg tcgttgccgc aaccgagtaa ttgaaattat
     6841 cagatgccgt ctgaaaccct gccgattgtc ggaatgtggt ttcagacggt atttgttttg
     6901 ttttccgtat attctgcaaa aatcgggatg gttgttttta tagtggatta aatttaaacc
     6961 agtacagcgt tgcctcgcct tgccttgccg tgctatctgt attgtctgcg gcttcgtcgc
     7021 cttgtcctga tttttgttaa tccactatat ttgcctgtgg tctttgcagg aatagtttgt
     7081 taacgaaatt tgatgcataa aatgcgccaa aaaatattgg ctgtgtttta atatagtgtt
     7141 gataagcgta cctggcttca atatactcaa taggatagat ctcttcctaa ccgaaaaaat
     7201 aattgtcttt tcccttgcaa ataattgaaa tcaacagatt actaaaacac agccaaaata
     7261 ttcaattgac aaaaactttt ctgattttga gaaatctgtg aaaatcaggg gattatttat
     7321 tttgaaaatg gtatcgggca taaaatttgg ctgtgtttca atatggtgct gataacatac
     7381 tttgtttcaa aatgttcaat aggataactt cctgattgac cgaaaaataa tggccttttc
     7441 ccttgcaagc agttgaaatc aacagattgt caaaatacat cctaaaaatt taataactta
     7501 tatatatgat tgcaaaggtt gcggcttgta taccggtcat tatggcggaa ataccgtttg
     7561 ggaggcagcc gcatcgcgtg gaatcggcac ggcaggcgat gcgccgatgc cttcgggcgt
     7621 gaaggcgggg caatcggcag tttggaaaaa ggcaatggaa tctgtgggca gaagaagtta
     7681 agggtaaaag tcgggcgcac ggtcaggttg tctgtccttt tgtcggaaca ggagactggg
     7741 cagctttgtc ctgattaatt gcttgtgcaa acggtaaaaa ggttttttcg tgtgttttaa
     7801 agcaggttgt acagcatttt tccggcaatc aaaagcaaca taatgccgaa ggattttttg
     7861 agtttggcag aagaaagttt gtgggcggtt ttgacaccga gcggggcaaa ggcaatggtt
     7921 gccgcgctga ggacggcgac ggcgggcagg taaaggaagc ccagtgaccc ttcgggcaat
     7981 cctgcaatat tcaggccgtt gagcagatac gatattgcgc cggagagtgc aatcggccag
     8041 gcaaggccgg atgatgtgcc gatggcttta tgggcgggga agccgcagtg gattaagaag
     8101 gggacggaaa gtgaaccgcc gcctatgccg acccagctcg acattgtgcc gaacagtgtg
     8161 gaaaccgcag tcagtccggg cagtccgggc agcgggcggg atgccgtctg agggtcggta
     8221 tgcagtgttt tgaatgcgac ggcggttaaa aacaggatga agaaaatttg aagcccgaac
     8281 gcggggatat attttgcgga gagtgcgccc gtgaatacgc cgaatatcat acccggcatc
     8341 atcgcaaata cggttttcca gtcgaccgcc tgttttttgt gctgccccaa catactggaa
     8401 aaggcggtga agaccatgac ggcgaaggat gtgccgacgg cgaggtgttg cgcgtaagga
     8461 tgttgtgcca aaccctgcaa atcaagtacc cataaaacga cagggacaat cagcgtgccg
     8521 ccgcctacgc cgaacagacc ggcaataaaa cctgccgcac tgcctacggc aagcaggatt
     8581 aagataatgt cccaatgcca catttaatcc gcctttctgt attttttcca gaatcggcgt
     8641 gccgccataa tgtagccgga caggctgtat ccgagaaaga acaggaagag gacgagcgac
     8701 ggttcccaag tgaccagaag cagcaataag acggcaagca gcattccgac aaaggggact
     8761 tgtctgcgga tgttgatttc tttaaaactc caaaaaggga tttggacaat catcgacagg
     8821 ccggcaaaca gtgtgatgcc caatgcccac cagtggacgg cggggaattt ttcgacgctg
     8881 tggttgaccc aaatcagccc gacaatcagc gcggcggcag tcggactggg cacgccgata
     8941 aaccagcgtt tgtccacctt gccgatgagt gtgttgaaca gggcgaggcg cagggcggcg
     9001 caggcgcagt agatgaaggc gacggaataa ccgattttgc cgaactgcca aagctgccat
     9061 ttgtaggcaa tcagagcggg agcaacgccg aagctgacca tatcggcaag gctgtcgagc
     9121 tgctccccga acgcgctttg gctgttggtc agccgcgcca cgcgcccgtc cataccgtcc
     9181 agcaacatag agatgaatac cgcgatggcg gcggtttcat aacgtccgtg catggattgg
     9241 gtgattgcgt aaaatgcgga aaacagcgcg gcgatggtaa aggagttggg cagcaggtag
     9301 atactgtttt gacgcaggga gcgttgcggg gtgttggtcg gggtttccat agctgaagtg
     9361 tctcacggac gtggagaatc gataggggat tataacccaa accattatcg aacaatacgc
     9421 cctttaggat gaagccctcg gaggcggttt gtttttctga tggcggatgc tttgcggggc
     9481 ggtttgactc tgcctgccac cgggtgtgtt gtttcggatt tttgccggat ggatattgtt
     9541 gctgggcatt tctgggcttg ccggaaggta tcggggggag ggcatcggaa tatgcggcgc
     9601 accgttcaaa ccggggcggc gtttccctat tttcaggggg ttgcaggttt caacaaattg
     9661 aaagatattt tttgcatttc cgaccgtatg ctgattttga agcgggataa attataggac
     9721 aaggtttttc gcccgccgca aaggttggcc gcaatgccgt ctgaaaacaa cggcttgccg
     9781 ctaaggcggc ggtatgcgga tttgcgctgc cgaaatcggt ttgccatacc cggcggtgca
     9841 atctgcgggg agggataagc cgataaggcg ggacttatcc tgttttttgt ttataatgca
     9901 acagaatttg gacgaaccgt cgggaattta tcatgatgaa aaagatactg gccgtatcgg
     9961 cactatgcct gatgactgcg gcggcacggg ctgccgatac ttacggctat ctcgccgttt
    10021 ggcagaatcc gcagaatgcg gacgatgttt tgcaggttaa aaccacaaaa gaagattcga
    10081 cgaaaagcga agcgtttgcc gagttggaag ccttttgcaa aggtcaggac acgcttgcgg
    10141 gcattgccga agacgagccg actggatgcc ggtcggtcgt gtcgctgaac aatacctgtg
    10201 tcgcgctggc atacccgaaa gccttgggcg tgatgcgcgt tgaaaacgcc gtcgtgatta
    10261 cttctccgcg ttttacgagc gttcatcagg tcgcactcaa ccagtgcatc aaaaaatacg
    10321 gcgtacaggg acaatgcggc ttggaaacag tgtattgcac atcttcttct tattacggcg
    10381 gaactgtgcg ctctttgatt caaaatctca aataaaacgg aaaatgccgt ctgaaagatg
    10441 ttcagacggc atttctatat cgacggtcag gattctttcg gatcgggcag caggctgttc
    10501 aacataatgg caagtacggc gcacaagccc acgccggcaa agctgaagct gcccaatttg
    10561 agcgtcatgc cgccgatgcc cgtggtcagt accgagctga cgatgaccag gtttttcggc
    10621 agcatcaaat cgactttggc atcaatcagc gttttcacgc ccaaagaagc aatcgtgccg
    10681 aacagcagca gcataatgcc gcccattact ggcatcggaa tggaagccaa aaacgcattg
    10741 aatttgccga aaaacgccat gcagacggca aaaaccgccg cccaagtcat gatgacgggg
    10801 ttgctgtttt tggtaatcat caccgcaccc gttacttcgc cgtaggtcgt aaccggcggg
    10861 ccgccgatca gacccgcaac gcatacgccc aaaccgtcgc ccgtaagggt tttgtccaag
    10921 cccgggtctt ttgtgtagtc tttccccgtt acattgccga ttgccatgat gccgccgatg
    10981 tgctcgatgg cgggggcgac ggcaacgggc agcataaaca gtgccgcctg ccagttgacc
    11041 tgaggtgttt caaaatgggg aacggcaaac cagggcgcgt gtgcaatgct tgccgtgtcc
    11101 accagtccca tcagcagtgc caaaacataa cccgaagcga caccgatcaa gatgggaatc
    11161 agcttcatca tcctgctgcc gaaaaccgat acgatggcgg taacggcaaa ggtaaagccg
    11221 gaaagaatca gcgaatcggt atagtcgatg acctgtttgc cgtccgcctg acccattgcc
    11281 atgctgcttg ccgccacggc aacagacaga ccgatgacca tgatgacggg gccgatgacg
    11341 accggaggca gcagtttgtg taccgctgcc agtccgcgcc aacggatcag cgcggcaaac
    11401 acaaaataca taaagccggc ggcaaacagt ccgaacatgg tggaaggcag cccccattcg
    11461 ccgacggagt agataatcgg tgcgataaag gcaaacgaag aaccaagaaa aatcggcact
    11521 ttgcgtttgg ttgtgatttg gaacagcagc gttcccaagc ctgcgcccaa aagcgcaaga
    11581 gccggattca gaccggtcag caggggaacc agcaccattg cgccgaatgc cacaaataaa
    11641 atctgcgcgc cggaaacggc aagtttcagt tggttcatac catcttcttt caatcggttc
    11701 aaacggcacg gattatactg gtttgccgag agtctgcgta aaaatacata tggcgggcaa
    11761 cagatatgcc gtctgaaagg caatgtctgt ctgttgcagg atgaaagcag gagaaatgga
    11821 gtataatccg acactttcca tattcaatcc gcaagccgtc cggcttcctt atttcagacg
    11881 gcattttccg gtaatacggc atgacagaaa aatcccataa aaaaacagca aaaggcaggg
    11941 cgggaagccc gtccccaacg tccgcacgca acaagaaagc cgacaacggc gcacggggca
    12001 ataaagtttc cgagcggctc aaggcggtca aagagttgca gaaaaccgaa accaaaaagg
    12061 cgcgtcccga acatgtcgtc aaccttatcg gcgacgcact gtggctgatg ggtttggcgg
    12121 caaccttgta tttggcgatt tccctgatta gtttcgatat gggcgatccg tcttggtcgc
    12181 acagttcgcc ggttgtggaa gatgtcgcca attggggcgg actgttcggc gcgtatgttg
    12241 ccgatgtcgg ctattatctt ttcggctggt cgttctggtg gtggatagcg gctgcctgcg
    12301 tcatgctgta taaaaatttt cgtctgcacg caaaacagac ggaaaacgag gcatacaacc
    12361 acaaaatcgc tgccgccgcg ctgtttgtcc tgacggtctt cagccccgtc ttggagtatt
    12421 ttgtgctggg cggaaaatat gccgactccc tgcctgtcgg agcaggcggt atggtcggca
    12481 tacgcgtcgg cgcagtgttt gcgtggctgc tggggaaatc gggcagcctg ctgattatct
    12541 tggttgttct gctgttgtcg ttgtccctgc tggtgcagat ttcatggctg gaatttttga
    12601 acggtgcggg cagggcggtt caaaaccgcc tgagtgcctt atccggcaag gtcatggctt
    12661 tgggaaaacg ccggccgaat accaaaacag acggtgtcga tacccaaaat acacggcgca
    12721 tggtaaaaga agccaagaat attacggcca aacccgttgc cttgcccgaa ggcagcagca
    12781 gcaaccgcaa atccgtcgcg gtttccgtcg cgccgccgcc caaaattcag gtttctctgt
    12841 ttgaagatga cgaacctcgg caggcgggcg aataccacaa gcctacattg aacctattgc
    12901 ggattcctga cagcgaaccc gtcagcatca atcccgccga attggagcgc actgccgaac
    12961 tgatcgaatc caaactggca gaattcggca tcggcgtaca agtcgtatcc gccacatccg
    13021 gccccgtcat cacgcgctac gaaatcgaac ccgcgcaagg tgttaaaggc agccaaattg
    13081 ttgccttgtc gaaagatttg gcacgctcta tgtcgctgca gtccgtgcgt atcgtcgaaa
    13141 ccatcgcagg taaaaacacg atgggcatcg agttgcccaa cgacaaacgc caagacgtga
    13201 tgttgagcga aatcttgtcc tcgcccgtgt ttgccggagc caaatccaag ctgaccgtcg
    13261 cgctgggcaa agacattgcc ggtacccccg ttgtcggcga cttggcgaaa atgccgcacc
    13321 ttttggtcgc cggtatgact ggttcgggca agtccgtcgg cgtgaacggc atgattatgt
    13381 ctatgctttt caaagctacg cccgacgaag tccgctttat catgatagac ccgaaaatgc
    13441 ttgagttgag catttacgac ggcattccgc acctgctctg tcccgtcgtg accgatatgc
    13501 gcgaagcggg gcaggcgttg aactggtgcg tcgccgaaat ggaaaaacgc taccgcctgc
    13561 tttcccacgc cggtgtacgc aatctggaag gcttcaacca aaaagtcgaa gccgcaaaag
    13621 cggcaggcaa gccgctgctc aatccgttca gtctgaaccc cgacaacccc gagcctttgg
    13681 aaaaactgcc gatgattgtg gtcgttatcg acgaacttgc cgacctgatg atgaccgaac
    13741 gcaaagccgt cgagcagcaa atcgcccgtc tcgcccaaaa agcgcgcgcc gccggtatcc
    13801 atatgattgt cgccacccaa cgtcccagtg tcgatgtcgt taccggcctg attaaagcca
    13861 acatcccgac gcgtatggcg tttaccgtgc aaagcaaaat cgacagccgt accatcctcg
    13921 accaaatggg cgcggacgaa ctgctcaaat atggcgattc gctgttcctc cagcccggca
    13981 gtgccgaacc gactcgcctg caaggcgcgt ttgtttcaga cgacgaagta catcaagtcg
    14041 tcaactacgt caaatcgcaa gccccagccg actatattga aggtctgctc agcggcgagg
    14101 ccgcgctgga aactgccaat atcgttaatc cgaatgtaga cagcgacgaa ttgttcgatc
    14161 aggcagtcgc ctatgttttg gaaagcaaaa aaacctccat ttcgtctttg cagcggcagt
    14221 tgcgtatcgg ctataaccgc gcggcaaacc tgatggaggc actggaaaat gcgggtgtgg
    14281 tttcgccgac cgatttgaac ggcagccgta aaattttggc gcacaaggac catttatagc
    14341 acccatatcg caaaatgccg tctgaacggc ggaattggcg tttcagacgg catattatgt
    14401 ttcaggcgga acattgtgat atacttgcca gctaaaatct cccctttgcg gcaatgcggt
    14461 tcaaatatcc gtaccgttgc gctgtttgct tccccatgca gggaagaagg tttaccattt
    14521 tatcaacaca acaaatttaa gggcttatga tgagcgtaac tgttgaaact ttagaaaatc
    14581 tggaacgcaa agtagtgttg tccctgcctt ggtccgaaat caacgcagaa accgataaaa
    14641 aactgaaaca aacccagcgc cgtgcaaaaa tcgacggttt ccgcccgggt aaagcacctt
    14701 taaaaatgat tgcccaaatg tacggtgcaa gtgcgcaaaa cgatgtcatc aacgaaatgg
    14761 tacaacgccg tttctacgat gttgccgttg cccaagaatt gaaagttgca ggctatccgc
    14821 gtttcgaagg cgttgaagaa caagatgata aagaatccct gaaaatcgct gcgattttcg
    14881 aagtattccc tgaagtcgtt atcggcgatt tgtctgcaca agaagtcgaa aaagtaaccg
    14941 cttccgtcgg cgatgccgaa gtggatcaaa ccgtagaaat cctgcgaaaa caacgcaccc
    15001 gcttcaacca tgtcgaacgc gaagcccgaa acggcgaccg cgccatcatc gactttgaag
    15061 gcaaaatcga cggcgaacct tttgccggcg gcgcatccaa aaactacgcc ttcgtattgg
    15121 gcgcaggtca aatgctgcct gaatttgaag ccggcgtagt cggcatgaaa gctggcgaaa
    15181 gtaaagacgt taccgtcaat ttccctgaag actaccacgg taaagacgtt gccggtaaaa
    15241 ctgccgtgtt caccattacg ctgaacaacg tttccgaagc gactctgcct gaagtcgatg
    15301 cagattttgc aaaagccttg ggtattgcgg atggcgacgt tgccaaaatg cgcgaagaag
    15361 tgcagaaaaa cgtaagccgc gaagtggaac gccgcgtaaa cgaacaaacc aaagaatccg
    15421 taatgaacgc gctgctcaaa gccgtagagc tgaaagcacc tgttgctttg gtaaatgaag
    15481 aagccgcacg cttggcaaac gaaatgaaac aaaattttgt taaccaaggt atggctgatg
    15541 ctgccaactt ggatctgcct ttggatatgt tcaaagaaca agccgaacgc cgcgtgtctt
    15601 tgggtctgat tttggccaaa ctggttgatg aaaacaaact ggaaccgact gaggagcaaa
    15661 tcaaagccgt tgtcgccaac ttcgcagaaa gctacgaaga tcctcaagaa gtaatcgact
    15721 ggtactacgc agagccttcc cgcctgcaag ccccgacttc tttggcgata gaaagcaacg
    15781 tcgttgattt cgttttgggt aaagccaaag taaacgaaaa agctttgtct tttgacgaag
    15841 tgatgggcgc gcaagtctga ttgttctgaa aatgccgtct tagggcagtt tttgaaagca
    15901 ccgaagccgc ttttaaagcc cgctttggtg cttttacatc atgaaaggag acgaaatgtc
    15961 ttttgataac tatcttgtcc ctaccgttat cgagcagagc ggtcgcggtg agcgtgcatt
    16021 cgatatctat tcccggcttt tgaaagagcg catcgtattc ttggtcggtc cggtaaccga
    16081 cgagtccgcc aatctggtgg ttgcccaact gttgtttttg gaaagtgaga atccggataa
    16141 ggatattttc ttctatatta actcgccggg cggttcggta acggccggta tgtcgattta
    16201 cgacaccatg aattttatca agcccgatgt atcgactttg tgcttggggc aggcagcaag
    16261 tatgggcgcg ttcttattgt cggcaggcga aaaaggcaaa cgtttcgccc tgcccaacag
    16321 ccggattatg attcaccagc ctttaatcag cggcggcttg ggcggtcagg catccgatat
    16381 tgaaattcac gcacgcgaac tgttgaaaat caaagaaaaa ctcaaccgcc tgatggcgaa
    16441 acattgcggc cgcgatttgg cagatttgga gcgcgacacc gaccgtgata atttcatgtc
    16501 cgccgaagag gctaaagaat atggtctgat tgatcaaatc ttggagaacc gtgcttcttt
    16561 acagttttaa taagaaatct gagagaaaca aatgccgttt gaaaacagtt caggacggca
    16621 tttgtcttat agggactgta tagggatgat tttgttctgg ttgcgtttgg agccgttttc
    16681 gccataattg gttttaacat attcctctac aatagctagg aattcagttg caatattatc
    16741 acctttcagt gtgactttgc gctccccgtc cacataaacc ggcgcgacgg gtgtttctcc
    16801 cgtaccgggc aggctgatgc cgatgtcggc caatttgctt tctccgggcc cattgacaac
    16861 gcagcccatc acggcaacat tcagggattc cacgcctgga taagcagtac gccatacgct
    16921 cattttttgt cgcaggtaat tttgaacatc ttgtgccagc tcttgaaata cggtactggt
    16981 ggtacgcccg cagccggggc aggcggtaac catcggcgta aacgaacgca atcccatagt
    17041 ctgtaaaatc tcttgcccga cgacgacctc ctgagtacgc gggctgccag gttccggagt
    17101 cagtgaaatg cggatggtgt cgccgattcc ttcttgaagc aagacggata atgccgccgt
    17161 tgatgcgaca atgcctttgc tgcccatacc ggcttcggtc aaacccaaat gcagcggata
    17221 ggcgcaacgg ctgcccagtt cgcggtaaac ctgaatcaaa tcctgaaccg cgctgacttt
    17281 gcacgacagg atgattttgt cttcgggcag tcccaataga acggcttttt cggcagattc
    17341 caaagcggag acaatcagtg cttccttcgt cacttcttcg ggcggtttcg gcgcggaaga
    17401 agcgaggttg gcatccatca tacgtttggc gaggctttga tccaaagaac cccagtttac
    17461 gccgatgcgg acggctttat cgttttcagc agcagtccga atcataaagg caaatttttc
    17521 atcgcctttt acgcctttgc cgacattgcc gggattgatg cggtatttgg acaatgcttt
    17581 gccgcattct ggaaattccg ccaacaggcg ttcgccgttg aagtggaaat cgccgataag
    17641 cggtgtggca tagcccatat cgtccaagcg gcggcggatt tcggcaactt tggacgcggc
    17701 ttcggggctg ttgacggtaa tacgcaccat ttcggatccg gcatcgctca attccttaat
    17761 ctgcaatgcg gtggcttttg catcggcagt gtcggtgttg gtcatagatt ggataacgac
    17821 gggtgcttct gaaccgacgg taatatgatc gatgcggact tgatgcgtct tgcggcgttg
    17881 gagtgtgttc atatggtttg aatctgttta ttgaccggtg aggacggttt gcaattcttc
    17941 cgagtagggg aaattcgcct gcaattgtgc ttcatattcg tatgccgcct gtgcgttgcc
    18001 gagggctttg gcaattttcc agcctagcag caaatcatcg gcctgaagga cttctaccct
    18061 gctttggtat tttttaaagt agtaatcggc atcgcccaac tgcccggcca gcattttggt
    18121 gcgcgccagt tctttaaatg cgggtgggaa ctgcggctgg gcggcgaggg aacgtttcaa
    18181 ataggcttcc gccaatccga attgcccctg ttttgcgctg catatgcctt tattcaggtt
    18241 ggcaatataa ggggtcgggt aggtggggtc ggccagggct ttgtcgaaat atgccataga
    18301 ttcggcaggg cggttgagcc tgccgcacag gaaccaaccg tagttgttgt tgatttcggc
    18361 actgtcgggt ttgatggaga gggcttgccg gaaacttttc tgcgccttgt cgttaacttt
    18421 caggtattgg tagatttcgg cacggaccag ccaggcaagc tcgtttttag ggtctgattt
    18481 cagggcgtct tcaatacttg ccgtcgcctg acggtagtcc tgaccgcgca tatattccat
    18541 tgccaactgg gttttgatat tggaaacctg attggctttt tctgcccgcg aggggcggta
    18601 ggaagtgctg cacgcgccca aggcaaggac aagtaataaa gagattcgtt tggatggctt
    18661 aaaaggcata attacccctg ttgtccgatt aaaatctgct gccatttttg ttggcggcgc
    18721 gttttatcct gaacctgccc cgccaactgt ccgcaggcgg catcgatgtc gtcgccgcgc
    18781 gtttttcgta cggtaacgac aaatcctgcc tgctgcaaaa tatcgcggaa cacacggatg
    18841 ttctcattgc tggagcgttc gtatccggag tttgggaagg gattgaacgg aatcagattg
    18901 aacttgcagg gaacatctgt gaccagttcg atcagttcgc gcgcatgttg cgccttatcg
    18961 tttattccgt ccaacatgac gtattcgaaa gtgatgaaat ccctgggtgc tttgaccaga
    19021 tagcgttggc atgcggccat caattctttc aagggatatt ttttgttcaa cggtacgatt
    19081 tggttgcgga cttcgtcatt ggaagcgtgg agggaaaccg ccaaagccac cggcatgaca
    19141 tcgcgcaacc tgtccatttg gggaaccata cccgaagtgg aaacggttac gcggcggcgg
    19201 ctcaaaccgt agccgtggtc gtccagcatg atgcttaagg cggtaacgac attgtcgaag
    19261 ttcgccatcg gctcgcccat gcccatcatg acgacgttgg aaatcacgcg ctcgtttttc
    19321 ggtgtaacgc ccatcgcttt gtttgcccac cacaattgcc cgatgatttc ggcagcagtc
    19381 aaattgcggt tgaagccctg ccggccggtc gaacaaaatg tacattccaa agcgcagccg
    19441 acttgtgagg aaatgcagag cgtgccgcga tccgattcgg ggatgaagac ggtttccacc
    19501 ccgttgcccg taccgacatc caaaagccat tttcgagtgc cgtctgaaga tttttgagac
    19561 atcatcagct tgggaatttc gatgcctgcc tgttcgttca gtttatggcg caacgatttt
    19621 gccaaatcgg tcatttcgtc aaaattttgc gcgccggatt ggtgcatcca acgcataacc
    19681 tgtttggcac ggaaaggttt ttcgcccata tcggcaaaat gtcgggtcag cccttggagg
    19741 tcgtagttga gcagattggt tttcatgtat ttgtgttttt cttaaattaa ggcttcaaat
    19801 aaaaaattgc agggcaggca agtttgattt gaagcctttt gcaacagagt tttcagacgg
    19861 catggatata ttttatgccg tctgaagagg agttgtgcgg tgtattgtat caacgggggc
    19921 agatttcggt ttggctgaaa aagtaggcaa tttccaaagc ggcattttcc ggagtgtccg
    19981 agccgtgtac ggcattgatg ctgaccgaag tggcaaagtc cgcgcgtatc gtgccttcgg
    20041 cggcttcggc gggattggtc gcccccatca gttcgcggtt tttcagaacg gcgttttcgc
    20101 cttccagtac ttgaatcata accggaccgc cggtcataaa ttcaaccaat ccggcgtaga
    20161 aggggcggtc tttatgaacc gcataaaatt cttgcgcctc tttgagggta agctgcttca
    20221 ttttggcggc aacgattttc agaccgttct cctcaaagcg gctgtatatt ttgccgatga
    20281 cgtttttgcc gacggcatcg ggtttgatga tggagatggt acgttcaatc gccatgctat
    20341 atccttattt ttacttaaga agaatcaaat cggtattcta tcaaaaaaat aacttatccg
    20401 cctgaatcct tgggagatag ggtcaggtgc ggctgttcca aataaacttc ggactgcatt
    20461 tcgaccatcc ggctggcggt gcgggtaaat tcttcggcaa aatcgccttc cgtatagata
    20521 tggttgacat ctaccgcgcc ggtggcacac agtttgaccc ggaaatcgta gagtacgtca
    20581 atcagccaag tcagccgccg cgcctccgcc ttttcttgcg gtgagagttg ttccaaacct
    20641 gaaataaaaa ccatttcata atgttcggcc aaatacagat agtcggactg tgagcggggg
    20701 ccgaagcaca gtgcgcggaa atcaaaccat atggcacggc cggactcggc tttgtgggga
    20761 atctcccgac cgtggatggt gctgatgccg gggttcaaat cggtaatgcc tgtcatttct
    20821 ttgaacagtt ttgccagttt tgcctcattt tcttcattgg caggcgtaaa gaaaatctcg
    20881 gcggggcgga gggtacgcag tcggtagtct tcaccgccgt caacgtttaa gacggtcagg
    20941 ctggactcga tgagcgcgat tgtgggaaga aaactgctcc ggttttgacc ttgcgggtag
    21001 agttcggaag gcgcgtagtt tgaagtcgcc accaaaacaa cgccctcgtt aagcaggttt
    21061 tccagcagac ggcctaaaat cattgcatcc gcaatatcgc tgacatgaaa ttcgtcaaaa
    21121 cataatacgc gggtttcttt ggcaatctcg gcggcaacgg atttcaacgg gttgctttcg
    21181 cttttcaggg ttttcagcct ctggtggatt tctgccataa aggcatgaaa gtggacgcgg
    21241 cgtttgcggc ggtaagggag gcagccgaaa aaagcgtcca tcagaaagct tttgccgcgt
    21301 ccgaccccgc catagaaata aagccctttg gggacttgcg gggaacgcaa actcctgcct
    21361 aaaaaacggt ttcttttgcg tttgaacatc atcaattcgg tccaaagccg atcgaggtgt
    21421 tcgatggcgg ctgcctgcgc gtcgtcgcgg atgaagttgg gcagttgtga ggcagcctga
    21481 taccaggtca gcgggctgtg gttttcaaac ggcggggctt taaaaagttg gtctctatta
    21541 ctcatattta accttgttat acttcttgcg gcggagaaag aaaatgctta ccgtcggtat
    21601 tataaatgat ttccttcaaa cttggcattt tccgtggaaa agaataatga cgcgcacaag
    21661 ggggtataag caaaggcggc gtttcaatat ggtgcagata tgagctcaaa tgccttttat
    21721 agtagattaa atttaaacca gtacagcgtt gcctcgcctt gccgtactat ctgtactgtc
    21781 tgcggcttcg tcgccttgtc ctgatttttg ttaatccact ataatccgta gagtcggctc
    21841 cttccggctc agacggcata cgtttatgcc ggcacttctt accgccctcg tgccggctat
    21901 cccaaaactg ctttgaacac tgaacgctct ttggtttact tcaaaaacgc ggcgtgataa
    21961 gcaatatgtt cgccaataaa actggcgatg aagtaataac tatggtcgta gcctttgtgg
    22021 aagcgcacat cgaccggctg gtttgccgca cgacaggttt cgataaaatc ttcggtacgc
    22081 aattgtgtcg gcaaaaactc atcttccaag ccctgatcaa tgcgtatacc ttgcacttta
    22141 tagccttgtt gaatgagcga gctggcatca tattgctgcc atttttcacg atcttcccct
    22201 aaataagcag taaaggcctt ttctccccaa ggcacgaggc ttggcgataa aataggcgaa
    22261 aaagcagaaa cgctttgata acgtttccga tttcgcagtg ccaataccaa tgcaccgtgt
    22321 ccgcccattg aatgccccat aatggaacgt ttgccgttag tagagaaatt tgcttcaacc
    22381 aaatcaggca attcgttcaa gatataatca tacatttgat aattcgtcgc ccaaggctgc
    22441 tcagttgcat taagataaaa gcccgcacct tgtcctaaat cgtaagccgc atcgttcggc
    22501 acttgctctc cgcgagggct ggtatcaggg gcaaccacaa tcacttgatg ttctgctgca
    22561 taaagctgaa agcctgattt agtaatgaaa ttttgttccg tacacgtcaa gccggaaagc
    22621 caataaatca caccaagcgg ttgattttcc gaattatttg gcaaatagac ggcaaatttc
    22681 atctcgcatt gaagcgtttg ggcatcatga gcccaaactt gctgcaaacc gccaaaaatt
    22741 tgatgttgtt caatcagttt catcgcatac cttagtagtg aataacggcg cggatcgatt
    22801 taccttcgtg cattaagtca aaggctttat tgatttgatc gagtgtcatt gtgtgggtta
    22861 caaacggttc taactcaatg tcgcctttca ttgaatcttc caccattttc ggcagttcag
    22921 agcgaccttt cacaccgcca aatgctgaac ctttccacac acggcctgtt accaactgga
    22981 acggacgcgt tgaaatttct tgtcctgcac ctgctacgcc gatgataatg gattgacccc
    23041 aaccacgatg tgcactttct aatgcttgac gcattacgtt tacattgccg atacattcaa
    23101 aggtatggtc aatgccccat ttgttgatgt ctaacaacac atctttaatt ggtttatcgt
    23161 aatcgttcgg gttcaaacaa tccgttgcac cgaactgttt tgccaactca aattttgatg
    23221 gattggtatc aatggcgata atgcggccgg ctttggcttg acgcgcacct tgcaccaccg
    23281 ccaaaccaat cgcgcctaag ccaaacacgg caacagagtc gccttcttgc acttttgccg
    23341 tattatgtac cgcaccaata cctgtggtaa cgccgcagcc gagcaaacat acttgttcat
    23401 ggttggcttc agggttgatt ttcgccagtg aaacttcggc aacaacggag tattcactga
    23461 aagtcgaaca gcccatatag tgataaatcg gctgaccttg ataagaaaaa cgcgtcgtgc
    23521 cgtccggcat taagccttta ccttgtgtat cacgcactga gacgcacaag ttggttttac
    23581 ctgaacaaca aaactcacat tcgccacatt cggcggtgta aagcggaatc acgtgatcac
    23641 ccggttttac gcttgacaca ccttcgccca cagcaacgac cacacccgca ccttcgtgtc
    23701 caagcaccac agggaatacg ccttcaggat cgcttcctga taatgtaaac gcatcagtat
    23761 ggcacacgcc agtgtgggta ttgcggatta acacctcgcc tttacgcggc atttctacgt
    23821 cgatttccac aatttgtaag ggttggttag gggcgaatgc caccgccgca cgagatttga
    23881 tgcttgaatc ggtttgtttc atttccattg tctttcctct taattcattg acaaagttgc
    23941 cttacctgag caacatagca acagtttaca ctttagagtt aactctaagg caagtgtttt
    24001 ttactg
//
LOCUS       DAXHTR010000009        23412 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.009, whole genome shotgun sequence.
ACCESSION   DAXHTR010000009 DAXHTR010000000
VERSION     DAXHTR010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23412
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.009"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(339..761)
                     /locus_tag="ACFNML_000273"
     CDS             complement(339..761)
                     /locus_tag="ACFNML_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFC6391725.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGAAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="ACFNML_000274"
     CDS             complement(879..1550)
                     /locus_tag="ACFNML_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HFC6391726.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPSGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDTVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1566..2192)
                     /gene="purN"
                     /locus_tag="ACFNML_000275"
     CDS             complement(1566..2192)
                     /gene="purN"
                     /locus_tag="ACFNML_000275"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFC6391727.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNRETAAGLQWA
                     AERGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCARYEGRL
                     MNIHPSILPSFTGLHTHERALEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3105)
                     /locus_tag="ACFNML_000276"
     CDS             complement(2299..3105)
                     /locus_tag="ACFNML_000276"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6391728.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAASEPAAASAAQGDTSSIG
                     STMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQEVMM
                     KFLQEQQAKAVEKHKAEAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGEGKQP
                     TKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEATFYI
                     PSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3364..3804)
                     /locus_tag="ACFNML_000277"
     CDS             complement(3364..3804)
                     /locus_tag="ACFNML_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFC6391729.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGRLQEL
                     DKMLWQYEAESFIPHEIWEMEEAMPSETSVLLACGGNPPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3867..5273)
                     /locus_tag="ACFNML_000278"
     CDS             complement(3867..5273)
                     /locus_tag="ACFNML_000278"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFC6391730.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDIHAFDEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHSAHEAVVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSAEA
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLIAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASYNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5443..6558
                     /gene="lptF"
                     /locus_tag="ACFNML_000279"
     CDS             5443..6558
                     /gene="lptF"
                     /locus_tag="ACFNML_000279"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFC6391731.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6555..7625
                     /gene="lptG"
                     /locus_tag="ACFNML_000280"
     CDS             6555..7625
                     /gene="lptG"
                     /locus_tag="ACFNML_000280"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFC6391732.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELTVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGLWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7935..10520
                     /gene="acnB"
                     /locus_tag="ACFNML_000281"
     CDS             7935..10520
                     /gene="acnB"
                     /locus_tag="ACFNML_000281"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFC6391733.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPGEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10662..11657
                     /locus_tag="ACFNML_000282"
     CDS             10662..11657
                     /locus_tag="ACFNML_000282"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFC6391734.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEIFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11881..12894)
                     /gene="ilvC"
                     /locus_tag="ACFNML_000283"
     CDS             complement(11881..12894)
                     /gene="ilvC"
                     /locus_tag="ACFNML_000283"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFC6391735.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12974..13267)
                     /locus_tag="ACFNML_000284"
     CDS             complement(12974..13267)
                     /locus_tag="ACFNML_000284"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFC6391736.1"
                     /translation="MSNIKIVALVTVKPEYTETLAAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAD"
     gene            complement(13334..13825)
                     /gene="ilvN"
                     /locus_tag="ACFNML_000285"
     CDS             complement(13334..13825)
                     /gene="ilvN"
                     /locus_tag="ACFNML_000285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFC6391737.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13836..15563)
                     /gene="ilvB"
                     /locus_tag="ACFNML_000286"
     CDS             complement(13836..15563)
                     /gene="ilvB"
                     /locus_tag="ACFNML_000286"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HFC6391738.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSSDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDTLGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
     gene            complement(16272..16356)
                     /locus_tag="ACFNML_000287"
     tRNA            complement(16272..16356)
                     /locus_tag="ACFNML_000287"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16320..16322),aa:Leu,seq:caa)
     gene            16632..17285
                     /locus_tag="ACFNML_000288"
     CDS             16632..17285
                     /locus_tag="ACFNML_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HFC6391739.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFNLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGGQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            17364..18017
                     /gene="hisG"
                     /locus_tag="ACFNML_000289"
     CDS             17364..18017
                     /gene="hisG"
                     /locus_tag="ACFNML_000289"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFC6391740.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            18121..19020
                     /locus_tag="ACFNML_000290"
     CDS             18121..19020
                     /locus_tag="ACFNML_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HFC6391741.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            19017..20306
                     /gene="hisD"
                     /locus_tag="ACFNML_000291"
     CDS             19017..20306
                     /gene="hisD"
                     /locus_tag="ACFNML_000291"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFC6391742.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAARRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            20352..21449
                     /gene="hisC"
                     /locus_tag="ACFNML_000292"
     CDS             20352..21449
                     /gene="hisC"
                     /locus_tag="ACFNML_000292"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFC6391743.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFARHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            21446..22363
                     /gene="hisB"
                     /locus_tag="ACFNML_000293"
     CDS             21446..22363
                     /gene="hisB"
                     /locus_tag="ACFNML_000293"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HFC6391744.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPNARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            22458..23327
                     /locus_tag="ACFNML_000294"
     CDS             22458..23327
                     /locus_tag="ACFNML_000294"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HFC6391745.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKALVEAAGGQFAEAPVSGSVGPATNGTLLILFGGSEAVLNPLQKIFSLVGKKTF
                     HFGDVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQVGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         5699 a         6505 c         5822 g         5386 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt caggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggcatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgcttg
      661 gccgcacctt ctgccgccga accctccata acgacgctgt gcccgccttc cgtcgtgccg
      721 acaaagcaca ttccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacacttga aaaccgattt acggtttggc ggactggccg
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgtatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccgag
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtagg ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcaggaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcgataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata aagtttatgc
     1681 tcgacagcca aaacccgtgc ggcaatatcg tctgccgtat cgccgtcaag tatcggcaca
     1741 atcccttgcg atacaatcgg gccgcaatcc agttcggcag taacaaagtg aatcgtacag
     1801 cctgcaacgc ggcaacccgc ttccaaagcg cgttcgtgcg tatgaagtcc ggtaaacgag
     1861 ggcaggatgg acgggtgaat gttcatcagc ctgccttcgt aacgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttt
     1981 tccatcatgg ccgtatcgaa ggcaagtcgt gattcaaagt ttttatgatt caggctatcg
     2041 gtcgggatgc cgcgttcggc cgcccattgc aaaccggcag ccgtttctct gttgctcaac
     2101 acggcggcaa tgcggacgtt gggaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccggaaa tcaggatgac gatgtttttc ataatggtac gcttttgaaa gggatgccat
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cgggccgatt ttgtcgcccg caccctgttc gcggtaggca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 gccctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgctt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcggtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcac cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgccctgcgc
     3001 ggcagaagcg gcggcaggtt cggatgcggc ttcttttttg ccacaggcgg aaagtgccaa
     3061 agcggcggaa agggtcagtg cgctgatttt gaaaatggtg ttcatgatgg atcttcgctg
     3121 tcgataaggt cggaaaaacg ggattatagc cgagtttgaa tgtttcaaca cacaggatga
     3181 cacataaagc gtcaatcgtg tgttgccctg ttttggaagg gattgaacct tccaaaataa
     3241 gttttgattc taccgccccg agggacagat gtccaagtgg cggggttcaa ccgataagga
     3301 aattttaatc aaatagaatc aagcctgttt aaattttgta aatgcggcat ttcagacggc
     3361 attttatgcc ttgccctcca tgccgtgatg ttcgatggca aaaccgcttc ggcggtaggc
     3421 ggtaaagcgt tcgcgcgcgt cggcgagatc ttccaagctg ttgccgacga tttccaaaac
     3481 gcgcgcgggc aagaccggag cggtgttcca aaaaccgtcg gacaggttca aaacagtcat
     3541 gccttcggga attcggggcg gattgccgcc gcaagcaagc aggacggatg tttcagacgg
     3601 catggcttct tccatttccc aaatttcgtg cggaatgaaa ctctcggctt cgtattgcca
     3661 aagcattttg tccaattcct gaagccgccc gaacgaatcg gaccacacca gtatcctgcc
     3721 gccgtcccga atcgcacggg caatcaggcg gcaggtgaaa atcggaactt gggcaacgtg
     3781 cgtgtaaaag gtggctttcg gcatattgtt tgagcatttg gcaggacgat gccgtctgaa
     3841 aggcttcaga cggcattgtg gcaaaattaa agattccgca gatagttcag cagcaaggga
     3901 acgggacggc cagtcgcacc tttttccgct ccggatttcc acgccgtacc cgcgatgtca
     3961 aggtgcgccc acgggtagcc ttcggtgaag taagacagga atgttgcggc ggtaatcgtg
     4021 cccgcgccgg gcgtgccgat gtttggaatg tcggcaaagt tggatttgag ttggtctttg
     4081 taggtctcaa agagcggcag ttgccatgct ttgtcgtcca cgttgtagga agcggcaagc
     4141 aggctgtcga tcaaatcctg attgttgccc atcacgccgc tgacatcgtg ccccaaggca
     4201 acaatacacg cgccggtcag ggtggcgacg tcgatgacgg ctttgggttt gaactgctcg
     4261 gcgtaagtga gcgcgtcgca caaaatcaga cggccttcgg catcggtgtt caacacttcg
     4321 atggtcagac ctttcatact tttcacgaca tcgcccggtt tgtttgccgc gccggaaggc
     4381 atattttcac aagtggcgac gacggcaatc aggttaatcg gcagttgcag tttgacggcg
     4441 gcgcagaagg tgctgatgac ggttgccgcg ccgcacatat caaacttcat ttcgtccatg
     4501 ttcaggccgg gcttgaggga gatgccgccg gtgtcgaagg taatgccttt gccgaccaat
     4561 accacaggcg cggcttcttt gtcggctgca ccgaaatagc tcagttcgac caaatagggg
     4621 gcttccgcgc tgcctttggc gaccgaccaa aacgaaccca tgttttcttt gatgtagtct
     4681 ttttcgatga ttttggcgtg cgcgcccagt ttttcggctt cggctttggc ggtgcgcgct
     4741 aaaaattcgg gcgtgcattc gttgggcgcg gcgttgccca agtcgcggca gaggctttgt
     4801 ccgtaaactt gcgcttcggc gacgcgcaag gcttctttaa cgacggcttc gtgtgcgctg
     4861 tggaacacgg cggtttcaaa tttggcgggc ttggcttctt ttttgtagcg gtcgaaacgg
     4921 taggcggcgt tgccgaacgc aatcgcaaac gcttcggcta cggcggcggc ttgcgcttca
     4981 tcgaaggcat ggatgtccac attgaccgtt tcctgatttt gcgcccattt ggcggcttcg
     5041 gcggcggcct tgttcaatgc ggcgcggccg gtgcttttca gacagcatac ggcaacagcc
     5101 tgcaaaccgt tgcctgtcgg gatttttgtg tcggcaaaat tttgaccttc ttcaagcgaa
     5161 gacaaaaggg caagggcggt cggatggttt aattgcgcct cttcggtgca gacaaataac
     5221 tgtgcgcctg cctgctgttc ctgcaagatt tcggtttttg tgctaaattc cacgtttatt
     5281 ctcctgattg agacggttgt cggtagtttt cggacggcct ttcgctcaaa agaccgtctg
     5341 aagacggctg gcacgattgt accccatttg aagcaccgtc tgaaaccttg cgcggacaat
     5401 ccgcctgcgc cgaaccgctt accgccccct gaccgcgatt ctatgattta tcaaagaaac
     5461 ctcatcaaag aactctcttt taccgccgtc ggcattttcg tcgtcctctt ggcggtattg
     5521 gtctccacgc aggcaatcaa cctgctcggc cgtgccgccg acgggcgtgt cgccatcgat
     5581 gccgtgttgg cattggtcgg cttctgggtc atcggtatga cgccgctttt gctggtgttg
     5641 accgcattta tcagtacgtt gaccgtgttg acccgctact ggcgcgacag cgaaatgtcg
     5701 gtctggctat cctgcggatt ggcattgaaa caatggatac gcccggtgat gcagtttgcc
     5761 gtgccgtttg ccgttttggt tgccgtcatg cagctttggg tgataccgtg ggcagagcta
     5821 cgcagccgcg aatacgctga aatcctgaag cagaagcagg aattgtcttt ggtggaggca
     5881 ggcgggttca acagtttggg caagcgcaac ggcagggttt attttgtcga aaccttcgat
     5941 accgaatccg gcatcatgaa aaacctgttc ctgcgcgaac aggacaaaaa cggcggcgac
     6001 aacatcatct tcgccaaaga aggtaacttc tcgctgaacg acaacaaacg cacgctcgaa
     6061 ttgcgccacg gctaccgtta cagcggcacg cccggacgcg ccgactacaa tcaggtttcc
     6121 ttccaaaaac tcaacctgat tatcagcacc acgccaaaac tcatcgaccc cgtttcccac
     6181 cgccgtacca ttccgaccgc ccaactgatt ggcagcagca acccgcaaca tcaggcggaa
     6241 ttgatgtggc gcatctcgct gaccgtcagc gtcctcctac tctgcctgct tgccgtgccg
     6301 ctttcctatt tcaacccgcg cagcggacat acctacaata tcttgattgc catcggtttg
     6361 tttttaattt accaaaacgg gctgaccctg ctttttgaag ccgtggaaga cggcaaaatc
     6421 catttttggc tcggactgct gcctatgcac atcatcatgt tcgtcatcgc aatcgtactt
     6481 ctgcgcgtcc gcagcatgcc cagccagccc ttctggcagg cggttggcaa aagtctgaca
     6541 ttgaaaggcg gaaaatgaac ctgatttcac gttacatcat ccgtcaaatg gcggttatgg
     6601 cggtttacgc gctccttgcc ttcctcgctt tgtacagctt ttttgaaatc ctgtacgaaa
     6661 ccggcaacct cggcaaaggc agttacggca tatgggaaat gctgggctac accgccctca
     6721 aaatgcccgc ccgcgcctac gaactgatgc ccctcgccgt ccttatcggc ggactggtct
     6781 ctctcagcca gcttgccgcc ggcagcgaac tgaccgtcat caaagccagc ggcatgagca
     6841 ccaaaaagct gctgttgatt ctgtcgcagt tcggttttat ttttgctatt gccaccgtcg
     6901 cgctcggcga atgggttgcg cccacactga gccaaaaagc cgaaaacatc aaagccgccg
     6961 ccatcaacgg caaaatcagc accggcaata ccggcctttg gctgaaagaa aaaaacagca
     7021 ttatcaatgt gcgcgaaatg ttgcccgacc atacgctttt gggcatcaaa atttgggcgc
     7081 gcaacgataa aaacgaattg gcagaggcag tggaagccga ttccgccgtt ttgaacagcg
     7141 acggcagttg gcagttgaaa aacatccgcc gcagcacgct tggcgaagac aaagtcgagg
     7201 tctctattgc ggctgaagaa aactggccga tttccgtcaa acgcaacctg atggacgtat
     7261 tgctcgtcaa acccgaccaa atgtccgtcg gcgaactgac cacctacatc cgccacctcc
     7321 aaaacaacag ccaaaacacc cgaatctacg ccatcgcatg gtggcgcaaa ttggtttacc
     7381 ccgccgcagc ctgggtgatg gcgctcgtcg cctttgcctt taccccgcaa accacccgcc
     7441 acggcaatat gggcttaaaa ctcttcggcg gcatctgtct cggattgctg ttccaccttg
     7501 ccggacggct cttcgggttt accagccaac tctacggcat cccgcccttc ctcgccggcg
     7561 cactacctac catagccttc gccttgctcg ctgtttggct gatacgcaaa caggaaaaac
     7621 gttgaaccaa tgccgtctga cccccttcgg acggcattgg tttttattat catattcctg
     7681 tagacagata gccgttccct attatagtgg attaacaaaa accagtacgg cgttgcctcg
     7741 ccttgccgta ctattgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
     7801 actatacatt acctgtcata acagttccat ttttgttaaa actagtctat gatagcggta
     7861 caaatattgt ttacaatatt taacgcaaat catttgcaac ccgacaaaag aaaaacagaa
     7921 aaaggaacaa agagatgtta gaagcctatc gtaaagccgc cgccgagcgc gccgccctcg
     7981 gcattcccgc cctccctttg aacgcgcagc aaaccgccga tttggttgag ctgctgaaaa
     8041 gcccgcccgc aggcgaaggc gagttcttgg tcgaactgct tgcccaccgt gttccgcccg
     8101 gtgtggacga tgccgccaaa gtcaaagcct cattcctggc tgccgttgcc gaaggcagcg
     8161 cgtccagccc gctgatctcc cccgaatatg cgaccgaact cttaggtacg atgctcggcg
     8221 gttacaatat ccacgcctta atcgaactct tggacgacga caaactcgcg cccattgccg
     8281 ccaaaggctt gaaacatacg cttctgatgt tcgattcctt ccacgacgtt caggaaaaag
     8341 ccgaaaaagg caacaaatac gcgcaagaag ttttgcaatc ttgggcagat gccgaatggt
     8401 tcgcctcacg cgccaaagtt cccgaaaaaa tcaccgttac cgttttcaaa gttgacggcg
     8461 aaaccaatac agacgacctc tcccccgcgc ccgacgcgtg gagtcgtccc gatattccgc
     8521 tgcacgcgct ggcaatgctg aaaaacccgc gcgacggcat cacgcccgac aaaccgggcg
     8581 aagtcggtcc gattaaattg ttggaagaac tcaaagccaa aggccatccg gttgcttacg
     8641 tcggcgacgt ggtcggtact ggttcttcac gcaaatccgc gaccaactcc gtcatttggc
     8701 ataccggcga agacattccg ttcgtgccga acaaacgctt cggcggcgta tgtttgggcg
     8761 gcaaaatcgc gccgattttc ttcaataccc aagaagattc cggcgcgctg ccgattgaag
     8821 tcgatgtatc tgctctaaaa atgggcgatg tcgtcgatat cctgccttat gaaggcaaaa
     8881 tcgtgaaaaa cggcgagact gttgccgagt ttgaattgaa atcacaagta ttgctggacg
     8941 aagtgcaagc cggcggccgt atcaacctga ttatcggccg aggtctgacc gctaaagcgc
     9001 gcgaagccct gaaactgcct gcctctactg cattccgcct gccgcaagcg cctgccgaaa
     9061 gcaaagctgg tttcaccttg gcgcaaaaaa tggtcggccg cgcctgcggt cttcccgaag
     9121 gacaaggcgt gcgtccgggt acttactgcg aaccgcgtat gacgacggtc ggctcgcaag
     9181 acacgaccgg cccgatgacc cgcgacgagt tgaaagactt ggcttgtttg ggcttctccg
     9241 ccgatatggt gatgcagtct ttctgccaca ccgccgccta tccgaaacct gtcgatgtaa
     9301 aaacccataa agaactgccc gcctttattt ccacccgtgg cggcgtgtca ctgcgtccgg
     9361 gcgacggcgt catccactcg tggctcaacc gcctgctact gcccgatacc gtcggcaccg
     9421 gcggcgacag ccatacccgt ttccccatcg gtatttcctt ccccgccggc tccggcttgg
     9481 ttgcctttgc cgccgcaacg ggcgtaatgc cgctcgatat gcccgagtct gtattggtac
     9541 gcttcagcgg caagctgcaa ccgggcgtaa ccctgcgcga tttggtgaac gccatcccgc
     9601 tgtacgcaat caaacaaggt ttgctgaccg ttgccaaagc cggtaagaaa aacatcttct
     9661 ccggccgcat cctcgaaatc gaaggcctgc ctgatttgaa agtggaacaa gcctttgaat
     9721 tgaccgacgc atccgccgaa cgctccgccg ccggctgtac cgtgaagctc aacaaagagc
     9781 cgattatcga gtacatgaaa tccaacgtcg tgttgatgaa aaacatgatt gccaacggct
     9841 atcaagaccc gcgcactttg gaacgccgca tcaaagctat ggaaaaatgg ctggcaaatc
     9901 ccgagttgct cgaagcggat aaagatgccg aatacgccgc cgtgattgaa atcaacatgg
     9961 acgacatcaa agagccgatt atcgcctgcc cgaacgaccc ggacgacgtg tgcttcatgt
    10021 ccgaacgctc cggcaccaaa atcgacgaag tattcatcgg ttcgtgtatg accaacatcg
    10081 gccacttccg cgccgcctcc aaacttttgg aaggcaaggc agacaccccc gtccgcctgt
    10141 ggattgcgcc gccgaccaaa atggacgcga aacaattgtc cgacgaagga cactacggcg
    10201 tactcggacg tgccggcgcg cgtatggaaa tgccgggttg ctccttatgt atgggtaatc
    10261 aggcgcaagt acgcgaaggt gcgaccgtta tgtccacctc cacccgcaac ttcccgaacc
    10321 gtttgggtaa aaacaccttt gtttacctcg gttcggcgga attggcagcg atttgctcca
    10381 aactgggtaa aatcccgacc gttgaagaat atcaagccaa tatcggcatc atcaacgaac
    10441 agggcgataa aatctaccgc tatatgaact tcaacgaaat cgacagctac aacgaagtag
    10501 ccgagaccgt gaacgtttaa tccccgtcat ccgtatgaag taagggattg accgcaatgc
    10561 cgtctgaaca accttcagac ggcattgcaa cattccgcta acccttcttt ccgcaaacgc
    10621 tgcaaatacg gcgttcacgc ccccacataa aggaaacgac agtgaacctg aaaaaccgcc
    10681 attttctgaa acttttagac ttcacgccgg aagaaatcac cgcctacctc gaccttgccg
    10741 ccgaattgaa agccgccaaa aaagcagggc gcgagattca gcggatgaaa gggaaaaaca
    10801 tcgccctgat ttttgaaaaa acctctactc ggacgcgctg cgcgtttgaa gtcgccgcgc
    10861 gcgatcaagg cgcgggagtg acttatttag agccgtccgc cagccaaatc gggcataagg
    10921 aaagcatcaa agacaccgcc cgcgtgttgg gcaggatgta cgatgccatc gaatatcgcg
    10981 gtttcggtca ggaagttgtt gaagaattgg cgaaatacgc gggcgtaccc gtgttcaacg
    11041 ggctgaccaa cgagttccat cccacacaaa tgcttgccga cgcactgact atgcgcgaac
    11101 acagcggcaa acctttgaac caaaccgcgt ttgcctacgt cggcgacgcg cgttacaaca
    11161 tgggcaattc cctgctgatt ttaggggcaa aattggggat ggacgtgcgt atcggcgcac
    11221 cgcaaagcct gtggccgtct gaaggcatta ttgccgccgc acacgccgcc gccaaagaaa
    11281 ccggcgcaaa aattaccctg accgaaaacg cgcatgaagc cgtgaagaat gttgatttta
    11341 ttcataccga tgtgtgggtc agcatgggcg agccgaaaga agtctggcag gaacgcatcg
    11401 atttgctgaa agattaccgc gttacgcccg aactgatggc ggcatcgggc aatccgcaag
    11461 tcaaattcat gcactgcctg cccgccttcc acaaccgcga aaccaaagtc ggcgaatgga
    11521 tttacgaaac cttcgggctg aacggtgtgg aagttacaga agaaatattc gaaagccccg
    11581 ccagcatcgt gttcgatcag gcggaaaacc gtatgcacac gattaaagcg gtaatggtcg
    11641 cggctctggg cgactgacag aactgtgcct gtttaaattc atccgcaaca cagataccgt
    11701 ctgaacacga tgttcagacg gtatccatat atagtggatt aaatttaaac cagtacggcg
    11761 ttgcctcgcc ttgccgtact atttgtactg tctgcggctt cgtcgccttg tcctgatttt
    11821 tgttaatcca ctataaaaaa actgcctaca cgatgtgtag gtagtcccgt ttgaaaacaa
    11881 tcagtttttg tcttggtcaa ccaatttgtt ggcagtaatc caaggcatca tggcacgcag
    11941 ttgtgcgccg actttttcaa cttggtggtc ggcattcaga cggcggcggg cagtcataga
    12001 cgcatagttg acattaccct cttggataaa catttttgcg tattcgccgg tttgaatgcg
    12061 tttcagggca ttgcgcatgg cttctttgct ggaagcattg accacttcag ggccggtaac
    12121 gtattcgccg tactccgcat tgttggaaat ggagtagttc atattggcaa taccgccttc
    12181 gaaaatcagg tcaacaatca gtttcatttc gtgcagacat tcgaagtaag ccatttcagg
    12241 cgcgtaaccg gcttcggtca gggtttcaaa acccgccttg atcaactcga ccacgccgcc
    12301 gcacaatacg gcttgttcgc cgaacagatc ggtttcggtt tcttcgcgga aagtggtttc
    12361 aatcacaccg cctttggtgc cgccgttggc agccgcataa gacagggcga tgtctttggc
    12421 tttgccggaa ttgtcttggt aaacggcaat cagagaaggc acgccgccgc cgcgtttgta
    12481 ttcactgcgt acggtatggc ccggaccttt gggggcaacc ataatcacgt ccaagtcggc
    12541 acgcggaacg atttggttgt agtgcacgtt gaagccgtgt gcaaatgcca gcgttgcgcc
    12601 ttctttcaaa ttggctgcaa cttcggcgtg atagacggca ggcatggttt cgtcaggcag
    12661 cagcagcata acgacatcgg cttctttggt cgcttcagca acggttttga cgacatggcc
    12721 ggctgcttcg gcttttttcc aagaagaacc ttggcgcaga ccaatcacca cgtttacacc
    12781 cgaatctttc aggttggcgg catgggcatg accttgcgaa ccgtaaccga tgatggcaac
    12841 ggttttgcct ttgattaggg acagatcggc atctttatcg taatagactt gcatttgatt
    12901 tcctttaagg taaatggttg tcgaagcctt aaaatgttga gcggcttcgg acgggttaaa
    12961 cagagtgtgc cgcttaatcg gcaacttcat tcatcaatac gatttccaac gcttcggttt
    13021 tgccgtcgac ggactggacg aaggcttgga aatgcgcgct ggcgttatgt tcgtcaatag
    13081 ctgcttgaga tttccaattt tccacgaaaa caaaacggtt cggtttgccg atttcctgat
    13141 ggagatcgta gctgatgttg ccctcttctg cacggctggc tttgaccagt tctttaaact
    13201 gtgctgccag tgtttctgtg tattccggtt tgacggtaac cagtgcgaca attttaatgt
    13261 tcgacataaa tctctcctgc cgttcgtttt tcagacggca ttcaaatacc gtgccgtctg
    13321 aaaggttacg gcgttaaatt ttcaaaatac gctcaccgcg accgatgccg gccgcgcctg
    13381 tgcgtacggt ttccaaaatt tgggcgcgtc cgaccgtttc caaaaaggaa tccagcttgt
    13441 ctgtcgagcc ggtaatttca atcgtatagc tgcggtcggt tacgtcgatg atgctgcccc
    13501 ggtagatttc ggtcaagcgt aaaaattcgt cgcggtcttt gccggcggca cggactttta
    13561 ccaacatcag ttcgcgttcg acaaaacggc tttcgttcaa atcgaccact ttaatcactt
    13621 caatcaattt attgagttgc ttggtgattt gttcgatgac ctgctcgtcg ccgtgggtaa
    13681 cgatggtcat acgggacagg gttttgtctt cggtcggcgc aaccgccaaa gaatcgatat
    13741 tgtaatcgcg tgcagagaac aaaccgacca cgcggctcat cgcacctgat tcgttttcaa
    13801 tcagaacaga taagatatgt cgcatttgcc tctccttacg ccttttcgtc cgcacgcata
    13861 tgcggcggaa gtaccatttc gtccaaacct ttgccgttac cgaccatggg catcacattc
    13921 tgtttctggt cggtcaggaa gtcgataaac accagcctgt ctttttggtt caatgcttcc
    13981 aacaacgcac cttccacatc agacttcttg tccacgcgga tgccgatatg accgtatgcc
    14041 tcggcaagtt tgacgaaatc gggcaaagaa tcgaaataag tttccgactc tcggccgccg
    14101 taatatattt cctgccactg gcgtaccata ccgagataac cgttgttcag cgtaatgacg
    14161 ttaaccggaa tccgatattg gaaacaggtg gacaactctt ggatgttcat ctgaatcgag
    14221 ccgtcgccgg taatacagaa tacgtcttga tccggggcgg caagttttgc accaatcgca
    14281 taaggcagac ccacgcccat cgtacccaaa ccgccggaat tgagccattg gcgcggacgt
    14341 tcgaagggat aatattgagc cgcaaacatt tgatgctgcc ctacatccga tgtgatgatt
    14401 gccgaattgc cggtaatctc ggcaagcttc tgaatcacat attggggctt gataatttcg
    14461 ctgccgttgt caaaccacaa gcaatctcgg gaacgccatt cctctatggt tttccaccat
    14521 ttgcccaaag tatcttcaga cggcacggac tcttgttttt gccacagcgc aaccatctcg
    14581 gacaaaacgt ttttcacgtc gccgacaatc ggaatgtccg ccttcacgcg tttggcgatg
    14641 ctggaaggat cgacatcgat atggataacc ttcttcgcct tctcgaaaaa tttggacggt
    14701 acggaaacca cacggtcgtc aaaacgcgca cctacggcaa gaacgacatc cgcattctgc
    14761 atggcaaggt ttgcctcgta agtaccgtgc ataccgagca taccgaggaa ttggcggtcg
    14821 ctggaaggat aagcgcccaa gcccatcagc gtacccgtgc acggagcacc cgtcattcgg
    14881 acaaatcggg tcagctcttc agaagcatta cccaacacca cgccgccgcc aaaatagacg
    14941 actggacgtt tggcagatgc caacatctgc acggcctttt taatctgacc gatatgccct
    15001 tgaacaaccg gttgatacga acggataaaa atgtcttcct gaggatagct gaatttcgcc
    15061 atcgcctgcg taacatcttt cgggacatca accaccacag gccccggtcg gccgcttgcg
    15121 gcaatttgga acgccttttt aatggtttcc gccaactcat tgatatccgt aaccaagaaa
    15181 ttgtgtttga cgcacggacg ggtaataccc accgtatcaa cttcttggaa cgcatccgta
    15241 ccaatcaggg aattgcctac ctgcccgctg atgaccacca tcggaatcga atccgtatag
    15301 gcagtagcaa taccggtcag tgcattggta acgcccgggc cggatgtaac caatgccacg
    15361 cccaccttac cgctgacgcg cgcatacgca tctgccgcgt gtactgccgc ctgctcgtga
    15421 cgggtcagaa tgtgcttgaa tttattgagt tggaaaaggg catcgtagat ttcgataacc
    15481 gcaccgccgg gataaccgaa aacgtactcg acaccttcgg ctttgagact ctgcactatg
    15541 atttgcgcgc ctgatagctg cataacgacc tcttttatac ggtttcaaac caatagggac
    15601 aaaccgcttt gccacagcac ctgtaatgca attccaccaa gcagcgattt agggtacgcg
    15661 cattggggga acacggcaac agacggatta tccaatcaat tggaaaggaa cacagagttt
    15721 gtgaaaaaga gtggaaacga taacgcaaac cgacagttca atcaagaaaa atctttcatc
    15781 ttttaatatt ttttgaaagc agagaaatta ttgattgatt ttaaaagaat aaaatcagga
    15841 gtaccttttt tgaaagatgg aaattgttga cagtttgtgt aggaggggca gatgtgaaaa
    15901 acccttcttc gatatcaaga attgtaaaat ttacagggtt tcatcccaat aaagactcgg
    15961 gatattgatt gaacttgatt tcatttttga tatatcaaaa atattcccaa ccatacttcc
    16021 tgaaaatggc tcattgcacc ggactgtatt ggacggcatt gacagaacca agagggctaa
    16081 caacgactta atatattgat tgtatagtgg attaacaaaa atcaggacaa ggcgacgaag
    16141 ctgcagacag tacaaatagt acggcaagga gaggcaacgc cgtactggtt taaatttaat
    16201 ccactatatt tagtcttatc tatttcatta aacagcaata gacaaaaaaa taaccgctcc
    16261 aaaagcggtt gtggtgccca gggtcggact cgaaccgaca caccttgcgg cgggggattt
    16321 tgagtcccct gcgtctacca atttcgccac ctgggctggt gaagaagtcg tcattataat
    16381 ggcttttgaa attctgtaaa cctttttttg aaattatttt atctgttttt attttatttt
    16441 tgattttaaa tagaattttt attattttaa tcttactgtt ctttccgctc caaagattct
    16501 gtatgattcg gcaattcctg ccgtgcagac aacgtaaaaa aatactacat taaatctgcc
    16561 aaacgcgtta agatgaaaat attcaaattc cgtacgaatc aggttttgct atttattctt
    16621 gggagattgt catgttttcc gtaccgcgtt cctttttgcc gggcgttttc gtacttgccg
    16681 cgcttgccgc ctgcaaacct caagacaaca gtgcggcgca agccgcttct tcaagtgcat
    16741 ccgcgtcagc tgcggaaaat gcggcaaagc cgcaaacgcg cggtacggat atgcgtaagg
    16801 aagacatcgg cggcgatttc acgctgaccg acggcgaagg caagcctttc aacctgagcg
    16861 atttgaaagg caaggtcgtg attctgtctt tcggctttac gcactgtccc gatgtctgcc
    16921 cgacagagct tttgacgtac agcgacacgt tgaagcagtt gggcgggcag gctaaggacg
    16981 tgaaagtggt gttcgtcagc atcgatccgg aacgcgacac gcctgaaatc atcggcaagt
    17041 atgccaaaca gttcaatccg gactttatcg gtctgacggc aacgggcggc caaaacctgc
    17101 cggtcatcaa gcagcaatac cgcgtggttt ctgccaaagt caatcaaaaa gacgacagcg
    17161 aaaactattt ggtcgaccac tcttccggtg cgtatctcat cgacaaaaac ggtgaggttg
    17221 ccattttctc gccttacgga agcgagccgg aaacgattgc tgccgatgta aggaccctgc
    17281 tctgataaaa ccgtatgccg tctgcaccgt cggcgcctat tcagacggca ttattgtttc
    17341 aaccgacaaa ggacatccac accatgcagg ataatgcttt gaccatcgcc ttatccaagg
    17401 ggcgcatttt tgaggagacg ctgccgctgc ttgccgctgc cggcattgtt ccgactgaag
    17461 agcctgaaaa atcgcgcaag ctgattatcg ggacgaacca tgaaaacatc cgccttgtca
    17521 ttgtccgcgc aaccgatgtg ccgacttatg tccgctacgg cgcggcggac ttcggcattg
    17581 cgggcaaaga cgtgctgatc gaacacggcg gcacggggct ttaccggcct ttggatttgg
    17641 agattgccaa gtgccgcatg atggttgctg tgcgtaaagg gtttgattac gaagcagctt
    17701 cgcaacccgg atgccgtctg aagattgcca cgaagtatcc tgaaatcgcg gcatctcatt
    17761 ttgccggcaa gggtgtccat gtggacatta tcaaactgta cggctcgatg gaacttgcgc
    17821 cgctggtcgg cttgagcgat gcgattgtgg acttggtttc gacgggcaac accttgaagg
    17881 caaacggctt ggaagcagtc gaacacatcg tcgacatttc cagccgcctg gtggtcaaca
    17941 aggctgcttt gaaaacgaaa tacgcgctgc tggagccgat tattcaggcg ttcggcggcg
    18001 cagtgaaggc gaagtaagca tccatttgaa taaagatgcg ttttcagacg accctatccg
    18061 ttcccgccga caggtcgtct gaaaatatca ccggcagtaa actgtatagg agaagttaaa
    18121 atggttgcaa aaataaaaaa attctcagat tcaacccttt ccgttttgaa taacggcgag
    18181 cgtcggtttt atgtctattg tctgaccgac ctgaaaaaag acaaaatcct ctacatcggc
    18241 aaaggctgcg gtaatcgtat cttcgagcat gaatgggttg ctagtcgttc acaagaccca
    18301 gtctccggcg agattatcga tcggaaactc aaagccatct ccaaatgcaa gaaactcggt
    18361 cgctatatca tcagctatca tctgactgaa gtcgaagcac tcgccgccga atctgcctta
    18421 attcattttg ttaaatctgt cttgggtaaa aaactcaaaa ataaaattgc cgggcatggt
    18481 ccgggtggta ttagcgtaga agaactagat cgccgctttg gattctcttc tctcccactt
    18541 aacgagatta accccgacgg gctgattctc gccatcaaaa tccacaatgc tttcgattta
    18601 gatactgacg aagaattaga ctaccttttc gacaaccaag acgatgccaa cctcaaatcg
    18661 cgtacgttgg gcaactgggt tatcggtaaa gatgttgctt caaaagtgaa atacgttatc
    18721 ggcgttcaca ccggtctgca aaacgctgtt gtcagtgcat acgaagtgga cggttttgaa
    18781 acaatggttg aggaaaccaa aaacggtaga aaacaatccc gttaccgttt ccgcactacc
    18841 tctcgtagcg aagaggtatt agccaaactc ggtctgcaac aaaaatgcct gcccgaattg
    18901 aagtttggta gcgggggaga aaaagcgtat atcagaccca aaacagagac agaaactgaa
    18961 caagagaata ttcagacgac ccccaatcca aaaataaaaa aggaaaaaac caaatcatga
    19021 aaaaactcaa cacccaatcg cccgatttcc aagccggact caaagccctg ctggcttttg
    19081 aaaccgcgca aaaccccgaa accgaacgca tcgtcgccga catttgcgcc gacgtgcaaa
    19141 agcgcggcga tgcggctttg attgaataca ccaacaaatt cgatcagaca aacgctaaaa
    19201 gcatcgatga tttaatactc acgcaagccg atttgaacgc ggcgttcgag cgcattccga
    19261 acgacgttca gacggcattg cagaccgccg cccgccgtgt cgaaagctac caccaacgcc
    19321 aaaaaatgga atcgtggagc tacaccgatg aagacggcac gctgttggga caacagatta
    19381 ccccgcttga ccgcgtcggc atttacgtcc ccggcggcaa ggcggcatat ccgagttccg
    19441 tcatcatgaa cgccatgccc gcccacgtcg caggcgtgaa agaaatcatc atggtcgtgc
    19501 cgacaccgaa aggcgaacgc aacgacatcg tgcttgccgc cgcatacgtc gccggcgtaa
    19561 ccaaagtctt caccgtcggc ggcgcgcagg cggttgccgc cctcgcctac ggcacggaaa
    19621 ccatccccca agtcgataaa atcaccggtc cgggcaacgc cttcgtcgcc gccgccaaac
    19681 gccgcgtgtt cggcgtggtc ggcatcgaca tggtggcggg gccgtctgaa atcctggtca
    19741 tcgccgacgg cacgacacct gccgattggg tggcgatgga tttgttcagc caggccgaac
    19801 acgacgaaat tgcccaagcc atcttaatcg gcacgtcgca agcgtatctc gacgaagtag
    19861 aagccgctat ggaccgcctg atcgaaacta tgccgcgccg cgacatcatc gaagcctcgc
    19921 tcggcaacag gggcgcgatg atactcgcca aagacttgga cgaagcctgc gaaatcgcca
    19981 actacatttc ccccgaacac ttggaactgt cagtcgaaaa tccgcaggaa tgggcgaaaa
    20041 aaatccgcca cgccggtgcg attttcatgg gacgctacac cggcgaaagc ctcggcgact
    20101 actgcgccgg tccaaaccat gtattgccca ccagccgaac cgcccgcttt tcctcgcctt
    20161 tggggacata tgatttccaa aaacgctcca gcctgattca ggtttcggaa cagggcgcgc
    20221 aaaaattagg cgaaaccgcc agcgtgctgg cacacggcga aagcctgacc gcccacgccc
    20281 gcgcggcaga gttccgtatg aaataatgcc gaaacggcgt acaggcatat tccaaccatt
    20341 aaggaaacac gatgaaatcc gtccgctcct tcatccgcga cgacatacaa gctatgtcgg
    20401 catatcagat tgccgacgtt ccgcccggct ttgccaaact cgattcgatg gaaagtcccg
    20461 tccacccttt tgccagacat gaaacgctgt tgcaggaatg gcaggcacgg cttgccgccg
    20521 cgcccatcca tctttacccc aatccctccg gcagcggttt acaggaagca ttacgttcgg
    20581 cgttcgacat tcccgactgc gccgacatcg cgctgggcaa cggctcggac gagctgatac
    20641 agttcatcac gatgctgacc gccaaaccgg gcgcggcaat gttggcagcc gaacccagtt
    20701 tcgtcatgta ccgccacaac gccgcgctgt acggcatgga ttatgtcggc gttccactga
    20761 acggagattt caccctcaac ctgcccgccg tcctcgaagc cgtcaggaag caccgccctg
    20821 ccctgacctt tatcgcctac cccaacaacc ccaccggcgt atgtttcacg cgtgccgaaa
    20881 tcgaagccgt catcgaagct tcagacggca tcgtcgtcgt cgacgaagcc tacggcgcat
    20941 tcaacggcga cagcttcctg ccgcaggcgg gcagcattcc caacctcatc gtcatgcgta
    21001 ccgtcagcaa aatcggtttt gccggactgc gtatcggcta tgcggcaggc tgtcccgaag
    21061 tcatcggcga actgcaaaaa atcctgccgc cctacaatat gaaccaactg agcctgacca
    21121 ccgccaaact cgccctgcaa cactacggca tcatctctgc caacatcgac agcctgaaaa
    21181 acgaacgcga acggatgttc gccgaattgg gcaaaatatg ccgtctgaac acctttccaa
    21241 gtcaggcaaa cttcattacc atacgcgtac ccgatgccga tttgttgttt gacacgctca
    21301 aacaaaaccg catcttggtt aaaaaactgc atggcgcgca cccgcttttg gaacactgcc
    21361 tgcgcattac cgtaggcagc cccgcacaaa acgatgccgt tctcaacatc attcgccaac
    21421 tttactgcca accaacggat ttcctatgaa tttgactaaa acacaacgcc aactgcacaa
    21481 ctttctgacc ctcgcccaag aagcaggttc gctgtccaag ctcgccaaac tctgcggcta
    21541 ccgtaccccc gtcgcactct acaaactcaa acaacgcctt gaaaagcagg cagaagaccc
    21601 aaatgcacgc ggcatccgtc ccagcctgat ggcaaaactc gaaaaacaca ccggcaaacc
    21661 caaaggctgg ctcgacagaa aacaccgcga acgcactgtc cccgaaaccg ccgcagaaag
    21721 caccggaact gccgaaaccc aaattgccga aaccgcatct gctgccggct gccgcagcgt
    21781 taccgtcaac cgcaatacct gcgaaaccca aatcaccgtc tccatcaacc tcgacggcag
    21841 cggcaaaagc aggctggata ccggcgtacc cttcctcgaa cacatgatcg atcaaatcgc
    21901 ccgccacggc atgattgaca tcgacatcag ctgcaaaggc gacctgcaca tcgacgacca
    21961 ccacaccgcc gaagacatcg gcatcacact cggacaagca atccggcagg cactcggcga
    22021 caaaaaaggc atccgccgtt acggacattc ctacgtcccg ctcgacgaag ccctcagccg
    22081 cgtcgtcatc gacctttccg gccgccccgg actcgtgtac aacatcgaat ttacccgcgc
    22141 actaatcgga cgtttcgatg tcgatttgtt tgaagaattt ttccacggca tcgtcaacca
    22201 cagtatgatg accctgcaca tcgacaacct cagcggcaaa aacgcccacc atcaggcgga
    22261 aaccgtattc aaagccttcg ggcgcgccct gcgtatggca gtcgaacacg acccgcgcat
    22321 ggcaggacag accccctcga ccaaaggcac gctgaccgca taaaaaacca taccgtctga
    22381 aacacccgca ggcttttcag acggtatcgg aacagataag attacactac actacaaaca
    22441 gaaaaggagt aaacatcatg tccgcaaacg aatacacaca aatcggctgg ataggcttag
    22501 ggcaaatggg tctgcctatg gtaacgcggc tcttggacgg cggcatcgaa gtcggcgtat
    22561 acaaccgctc gcccgacaaa actgccccca tctccgccaa aggcgcaaaa gtttacggca
    22621 acaccgccga actcgtccgc gactatcccg tcattttcct gatggtttcc gactatgccg
    22681 ccgtgtgcga catcctgaac ggagtccgcg acggattggc cggcaaaatc atcgtcaaca
    22741 tgagcaccat ctccccgacc gaaaacctcg ccgtcaaagc acttgtcgaa gccgcaggcg
    22801 gacagtttgc cgaagcaccc gtttccggat cggtcgggcc cgccaccaac ggcacgctgc
    22861 tgattctgtt cggcggcagc gaagccgttt taaacccgct gcaaaaaata ttttccctcg
    22921 tcggcaaaaa aaccttccat ttcggcgatg tcggcaaagg ttcgggcgcg aaactcgtct
    22981 tgaactcgct cttgggcatt ttcggcgaag cgtacagcga agcgatgctg atggcgcggc
    23041 agttcggcat cgataccgac accatcgtcg aagccatcgg cggctcggca atggactcgc
    23101 ccatgttcca aaccaaaaaa tccctgtggg caaaccgcga attcccaccc gccttcgccc
    23161 tcaaacacgc ctccaaagac ctcaacctcg ccgtcaaaga gcttgaacag gtaggcaaca
    23221 ccctgcccgc cgtcgaaacc gttgctgcca gctaccgcaa agcagtcgaa gccggctacg
    23281 gcgaacagga cgtttccggc gtttacctga aattggcaga acactgattg ccttttccaa
    23341 acacaatgcc gtctgaacat atttcagacg gcatttttat caccccacgc ttaaaatcag
    23401 tcccgattat ga
//
LOCUS       DAXHTR010000010        23310 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.010, whole genome shotgun sequence.
ACCESSION   DAXHTR010000010 DAXHTR010000000
VERSION     DAXHTR010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.010"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            46..1722
                     /gene="pilB"
                     /locus_tag="ACFNML_000295"
     CDS             46..1722
                     /gene="pilB"
                     /locus_tag="ACFNML_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HFC6391746.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYKESQAGKEVLPILFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(1807..2016)
                     /locus_tag="ACFNML_000296"
     CDS             complement(1807..2016)
                     /locus_tag="ACFNML_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFC6391747.1"
                     /translation="MTESRQTRLQVKCPTCQTAVVWEPENAFRPFCSQRCKLIDLGGW
                     ADGKYTVSGQTESLPEISEPDMAYR"
     gene            complement(2009..2641)
                     /gene="coaE"
                     /locus_tag="ACFNML_000297"
     CDS             complement(2009..2641)
                     /gene="coaE"
                     /locus_tag="ACFNML_000297"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258461.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFC6391748.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVADIISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHND"
     gene            complement(2643..3503)
                     /locus_tag="ACFNML_000298"
     CDS             complement(2643..3503)
                     /locus_tag="ACFNML_000298"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFC6391749.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSIWLLCAVYKLIKGEIGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            complement(3577..4809)
                     /gene="pilG"
                     /locus_tag="ACFNML_000299"
     CDS             complement(3577..4809)
                     /gene="pilG"
                     /locus_tag="ACFNML_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HFC6391750.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            5319..6962
                     /gene="pgi"
                     /locus_tag="ACFNML_000300"
     CDS             5319..6962
                     /gene="pgi"
                     /locus_tag="ACFNML_000300"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6391751.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGTTGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHITVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETACHFCAVSADTAAAAAFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPPRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPVWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            7129..7950
                     /gene="dapD"
                     /locus_tag="ACFNML_000301"
     CDS             7129..7950
                     /gene="dapD"
                     /locus_tag="ACFNML_000301"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFC6391752.1"
                     /translation="MSLQNIIETAFENRADINPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEILNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQASPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPAGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            8105..8890
                     /gene="fabI"
                     /locus_tag="ACFNML_000302"
     CDS             8105..8890
                     /gene="fabI"
                     /locus_tag="ACFNML_000302"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFC6391753.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELGSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(9075..10073)
                     /gene="ilvE"
                     /locus_tag="ACFNML_000303"
     CDS             complement(9075..10073)
                     /gene="ilvE"
                     /locus_tag="ACFNML_000303"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFC6391754.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYSSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALIE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVDELKAAVENGAEA
                     ILTGTAAVISPVTSFVIGGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            10383..10697
                     /locus_tag="ACFNML_000304"
     CDS             10383..10697
                     /locus_tag="ACFNML_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly LapA
                     domain-containing protein"
                     /protein_id="HFC6391755.1"
                     /translation="MKLIYTVIKIIILLLFLLLAVINTDAVTFSYLPGQKVDLPLIVV
                     LFGAFVVGIVFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            10708..11877
                     /gene="lapB"
                     /locus_tag="ACFNML_000305"
     CDS             10708..11877
                     /gene="lapB"
                     /locus_tag="ACFNML_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFC6391756.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            11902..12318
                     /gene="gloA"
                     /locus_tag="ACFNML_000306"
     CDS             11902..12318
                     /gene="gloA"
                     /locus_tag="ACFNML_000306"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004462 - lactoylglutathione lyase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0051596 - methylglyoxal catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFC6391757.1"
                     /translation="MRLLHTMLRVGNLEKSLDFYQNVLGMKLLRRKDYPEGRFTLAFV
                     GYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDAYEACERVKRQGGNVVREAGP
                     MKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(12491..15016)
                     /gene="tspA"
                     /locus_tag="ACFNML_000307"
     CDS             complement(12491..15016)
                     /gene="tspA"
                     /locus_tag="ACFNML_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_164729270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark-like protein
                     TspA"
                     /protein_id="HFC6391758.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     DGLFGGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPDLDDAADDGIEI
                     TFAEVETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLA
                     AAQNGILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESV
                     AQTAENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTV
                     AEVQTPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETV
                     AETPDFNATADDLSALLQPSKVPAVEENAAETVADDLSALLQPAEAPAVEENVTETVA
                     ETPDFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENATEITLE
                     TPDSNTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTGFPSESVGSDAPSEAKY
                     DLAEMYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            15324..15854
                     /locus_tag="ACFNML_000308"
     CDS             15324..15854
                     /locus_tag="ACFNML_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFC6391759.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVIFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            15857..16147
                     /locus_tag="ACFNML_000309"
     CDS             15857..16147
                     /locus_tag="ACFNML_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFC6391760.1"
                     /translation="MEYFMLLATDGENVYEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            16147..16425
                     /locus_tag="ACFNML_000310"
     CDS             16147..16425
                     /locus_tag="ACFNML_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6391761.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            16474..17340
                     /locus_tag="ACFNML_000311"
     CDS             16474..17340
                     /locus_tag="ACFNML_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6391762.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            17411..18169
                     /locus_tag="ACFNML_000312"
     CDS             17411..18169
                     /locus_tag="ACFNML_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6391763.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQAIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            18243..18734
                     /gene="dtd"
                     /locus_tag="ACFNML_000313"
     CDS             18243..18734
                     /gene="dtd"
                     /locus_tag="ACFNML_000313"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFC6391764.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DAHYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADAASGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            18765..19775
                     /gene="dusA"
                     /locus_tag="ACFNML_000314"
     CDS             18765..19775
                     /gene="dusA"
                     /locus_tag="ACFNML_000314"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFC6391765.1"
                     /translation="MNDNTHTLPPRRLSVAPMLDWTDRHYRYMARQITRNAWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEAYGYNEVNLNCGCPS
                     PRVQKGSFGACLMNEVGLVADCLNAMQDAVGIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EAIAGHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTANPIGYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKGARTWRRMLSDATLLKDNDGSLILEAWKEVERANT
                     RE"
     gene            complement(19845..20732)
                     /gene="gluQRS"
                     /locus_tag="ACFNML_000315"
     CDS             complement(19845..20732)
                     /gene="gluQRS"
                     /locus_tag="ACFNML_000315"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFC6391766.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARAHGGRWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWYGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQAAAVHGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLGVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMDKVPKHAITTP"
     gene            complement(20749..21039)
                     /locus_tag="ACFNML_000316"
     CDS             complement(20749..21039)
                     /locus_tag="ACFNML_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391767.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYTELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            complement(21044..21178)
                     /locus_tag="ACFNML_000317"
     CDS             complement(21044..21178)
                     /locus_tag="ACFNML_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391768.1"
                     /translation="MPSERLSDGIALLTGHSAAYQGFALKILMGKKLMHDITRQHKRK
                     "
     gene            complement(21193..22248)
                     /gene="tal"
                     /locus_tag="ACFNML_000318"
     CDS             complement(21193..22248)
                     /gene="tal"
                     /locus_tag="ACFNML_000318"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFC6391769.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLTPKQRYETMAVADVRAACGVCLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRAQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDSALDTTLPDRLK
                     GKIAIALAKAAYQDWEQYFTAPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDSL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLATRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            22345..>23310
                     /locus_tag="ACFNML_000319"
     CDS             22345..>23310
                     /locus_tag="ACFNML_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HFC6391770.1"
                     /translation="MAGNEKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKDITLVCITARPDSTMARHADIHITASVSKEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTISAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAAR"
BASE COUNT         5204 a         5996 c         6677 g         5433 t
ORIGIN      
        1 aagctgtaca ggtataacaa tgaataaatt tggggataag gtcgtatgag cgtaggtttg
       61 ctgaggattc tggttcaaaa ccaggtggtt actgttgagc aggccgagca ttactacaag
      121 gagtcgcagg cgggtaagga agtgttgccg atactgtttt cagacggcgt catttcgccc
      181 aagtcgcttg cggcattgat tgcaagggtg ttcagttatt cgattcttga tttgcgtcat
      241 tatccgcgcc acagggtgct gatgggggtg ttgacggagg agcagatggt ggagttccac
      301 tgtgtgccgg ttttccgtcg gggcgacaaa gtattttttg cggtttccga tccgacacag
      361 atgccgcaaa ttcagaaaac cgtttctgcc gcagggattg aggttgagtt ggtcattgtc
      421 gaggatgacc agttggcggg tttgctcgat tgggtgggtt cgcgttcgac atcgctgctt
      481 caggagcttg gggaggggca ggaggaagag gaaagccaca ccctgtatat cgacaacgag
      541 gaggcagaag acggccctgt tccgaggttt atccataaga ctttgtcgga tgccttgcgc
      601 agcggggcat cggacatcca tttcgagttt tacgaacaca atgcccgtat ccgtttccgt
      661 gtggacgggc agctccgcga ggtggttcag ccgcccattg cggtaagggg gcagcttgct
      721 tcccggatta aggtaatgtc gcgtttggac atttccgaaa aacggatacc gcaggacggc
      781 aggatgcagc tgacctttca aaagggcggc aagcctgtcg atttccgtgt cagcacattg
      841 ccgacgctgt ttggcgaaaa ggtcgtgatg cggattttga attccgatgc cgcgtctttg
      901 aacatcgacc agctcggttt tgagccgttt cagaaaaaat tgttgttgga agcgattcac
      961 cgtccctacg ggatggtgct ggtaaccggt ccgacgggtt cgggtaagac ggtgtcgctc
     1021 tatacctgtt tgaatatttt gaatacggag tcggtaaata ttgcaacggc ggaagaccct
     1081 gccgagatta acctgccggg catcaatcag gttaacgtca atgataagca gggcctgact
     1141 tttgccgctg ctttgaagtc tttcctgcgt caggacccgg acatcattat ggtcggtgag
     1201 attcgtgatt tggaaactgc cgatattgcg attaaggcgg cacaaacagg gcatatggtg
     1261 ttttccaccc tgcacaccaa taatgcgccg gcgacgttgt cgcgtatgct gaatatgggt
     1321 gtcgcgccgt ttaatattgc cagttcggtc agcctgatta tggcgcagcg tcttttacgc
     1381 aggctgtgtt cgagctgcaa acaggaagtg gaacgcccgt ctgcctctgc tttgaaggaa
     1441 gtcggcttca ccgatgagga ccttgcaaaa gattggaaac tttaccgcgc cgtcggttgc
     1501 gaccgttgcc gggggcaggg ttataagggg cgtgcgggcg tgtatgaggt tatgcccatc
     1561 agcgaagaaa tgcagcgtgt gattatgaac aacggtacgg aagtggatat tttggacgtt
     1621 gcctataagg agggtatggt ggatttgcgc cgggccggta ttttgaaagt tatgcagggc
     1681 attacttcat tggaagaggt aacggcaaat accaacgatt aggtttgaga atgaaaatgc
     1741 cgtctgaagc gtgtttgttt cagacggcat ttgactttca gggtgtttgc cgggaaggcg
     1801 gggcggtcag cggtatgcca tgtcgggttc ggatatttcc ggcaaacttt ccgtttggcc
     1861 ggaaaccgta tatttcccgt ctgcccatcc gcccaagtcg atcagtttgc agcgttgcga
     1921 acagaagggg cggaatgcgt tttcgggttc ccatactact gctgtttgac aggtcggaca
     1981 tttgacttga aggcgtgttt gccgcgattc agtcattgtg ttttccttgt gttggttttg
     2041 aggcgaaaat ccctgaataa aacgcgtgca ggcgcattgt tttctcacgc aggcttttga
     2101 ggctgccgtc attgagcagc acatcgtctg caagcagcag gcgttcggat tcggatgcct
     2161 gatggctgat gatgtccgcc acctcgccgc gcgtcagccc gctgcgggcc atcaccctgc
     2221 cgatacgttt ttccaaaggt gcgcttatgg tcaggacacg ccgtatcagg ctgataaatt
     2281 gacgcttttc cgtcagcagc ggaatttcga caatgccgta agccgcatcg gtaaaggttt
     2341 cttgctgttt tttgatttct gagaaaatca gcggcaacat cacggattcg agcaaggctt
     2401 ttcgcgatgg ggaggcaaag acttctttac gcaatatgtc gcgtcgcaac aaaccctgtg
     2461 tgtcgaaaac ggtgtcgccg aacagccgcc tgatttccgg cagggcgatg ccgtctgaag
     2521 ccgtcagcga gtgcgccgcc gcgtccgcat cgatgcgcgg cacgcccaaa tcggcaaaac
     2581 attgcgcggc tgccgatttg ccgctgccga ttccgccggt cagtccgacc cataccgtca
     2641 tcttacagca ccggatgggt cagccaccag ttgaccgccc gccatacgga atcgtttgcc
     2701 gtaaaaatta tccagcccga aactgtcagt gcggggccga aggcaaaatg ctgccccttg
     2761 gcgacgcgca taacgattgc cgcgaccaaa ccgatcagcg aggaaacaaa aatcagcacg
     2821 ggcaatgcgg atatgccgag ccacgcgccc aatgcggcaa tcagtttgaa atctccgttg
     2881 cccataccga tttcgccttt aatcagttta tataccgcgc ataaaagcca tatcgaacca
     2941 tagccggcaa ccgcacctaa aacggcagac tgcaaaggca cgaagccgcc gtccaaatta
     3001 aatatcagcc ccagccaaat caagggcagt gtcatcgagt cgggcaggta ttgggtgtcc
     3061 gcatcgataa aggtcaggga aatcagaaac gcggtcagta ccaatccgcc cagcgtaatc
     3121 caagaccagc cgtattgcca ggcgaccagc ccgaacaata cgccggtcag cagctcgatt
     3181 aagggataac gtatgctgat tttggtttga caggaggcgc atttgccgcg caggagcagg
     3241 taactgacaa tcgggatgtt ctgccacgcg cgtatcggca cgcggcattt gggacagcag
     3301 gaatccggtt tcatcaggtt gaaggtacgg ctttcctctt cggtcagcgg caggtttaaa
     3361 tattctttgg caaataccgt ccagccgcgt tccatcatga ccggcacgcg gtaaatgacg
     3421 acgtttaaga aactgccgac cagcagcccg aacaccgctg ccaaaggcac ggcaaacggc
     3481 gacaatacag acaaatcaga catattttgt tctcaatgta ttcaaaacaa aaacaaaccg
     3541 gcgcagagcg aatccgcgcc ggatctgtgc ggcaaatcag gcgaccacgt tgcccaaatt
     3601 aaacagcggc agatacatgg cgaccagaag cgtgccgatg accaagccta aaatcacgat
     3661 aatgatcggc tccatcatag cggacagcct gccgaccgca ttgtccacct cgtcttcgta
     3721 aaattcggcg gctttgttga gcatatcgtc caaagaaccc gattcctcgc cgatggaaga
     3781 catctgcaac atcatattgg ggaacagttc cgtcgcacgc atccccgaag tcatagacaa
     3841 accttggatg acgcgcgtac ggatttcccg ggtggcttct tcatagatta aattgcccgc
     3901 cgcgccggca gtggagtcca atacatcgac caaaggcacg cctgccgcaa tcagcgtcgc
     3961 cgtcgtcctg ccccagcggg caatcgtccc tttgcggaca atgtctccga aaatcggcat
     4021 acgcagcagt atggcatcca tacgccgttg gattttaatc gaacgcgcct tcaatttaag
     4081 gaagccgtat atggcaaagc ccagtgcgat cagcaccatc cagccgtatg agacgaaaaa
     4141 gtcggacata tccatcactg tttgggtcag tgcgggaagc tccgcgccca tattggcgta
     4201 aacttcttta aaggcgggca gtacgaaaat catcatcacg aataccaaac cgatggcgac
     4261 ggcgatgacg gataccggat aggtcagtgc ggtttttacc tttttgcgga tggcctgggt
     4321 tttttctttg taaattgcca atttgtccag caggctttcc aatacgccgc ccgtttcgcc
     4381 cgccgcaacc agattgcagt agaagcggtc gaaatatttt gggtggtttg agaatgcgcg
     4441 gctcaacgag ctgccctgtt ccacttcgcc tcggatttcc atcagcattt ccgtcataga
     4501 cgggttgccg tgtccgcgcg ccacgatttc aaatgcctgc atcagcggca ggcccgcttt
     4561 aatcatcgtg gacagctggc gggtgaaaac ggtgatgtct tcttgtgtga ttttgcgctt
     4621 ggagcttgtt ttcacacggg taatctgcaa cgggcggatg ccgcgttttg ccagtttttt
     4681 gcgcgcctct tcttcggtaa acgcggatac ttcgccgttg accagtttgt cggaggcgga
     4741 atgcctgcct tcaaagataa agcgtttttc tttctttgcg aacaaagaaa atcctccgtt
     4801 tttagccata ttctagcccc gtaaagtaat tggaataaaa tgtaagaaac atcgttaaaa
     4861 aacagtaccg gcgtgttccc ggtaagatga aaaccgccga catcccgcct gcgggcggca
     4921 aacgggacag aatcggatgc gattatacct tatttaggcg gctgtccggc atttatgcgt
     4981 acacaataaa tcttgcagga tattgttgcg ggtcaaatgc cggccggagg gtatagtgaa
     5041 ttaacaaaaa ccggtacagc gttggctcgc cttagctcaa gagagaacga ttctctaagg
     5101 tgctgaagca ccaagtgaat cggttccgta ctatttgtac tgtttgcggc tcgccgcctt
     5161 gtcctgattt ttgttaattc actatatttt cgccatatgg aaataaggtg ctattggacg
     5221 cggcgggcgg tgttccggag attcgccaaa gccgctgccg tttgttaaac tacattctgc
     5281 tacattttaa tccggttctg aaaaatcaag gaaaacagat gaatgctttt acccgtgcat
     5341 ggtatgcgct cgaacgccat tatcaggata cgcgtcatgt ccttttgcgc gaccgctttg
     5401 cctgcgaacc cgaccgtttt gagcgtatgc atgagcgttt ggacgggatg ttgttcgatt
     5461 acagcaaaaa ccgtttgggc gaagatacgc tgcaactgct ctgccgtctt gcggagacgg
     5521 cggatttgga agggaaaatg cgtgctttgc ggacgggtgc gaaagtcaac ggcagcgagg
     5581 ggcgtgccgc gctgcatacg gctttgcgcc tacccgacgg tgcggatgcc gtttatgcgg
     5641 acggcaggga cgtgttgccc gaaatccgcc gcgagttaaa tcgtgcgttg gagtttgcac
     5701 acagtttgga cgacggttcg tatcagggga caaccggaaa acggattacg gattttgtcc
     5761 acatcggcat aggcggatcc gacctcgggc cggcaatgtg cgtgcaggca cttgagccgt
     5821 tcagacggca tatcaccgtc cattttgccg ccaacgccga tcctgcctgc ctggatgcgg
     5881 ttttatgccg tctgaacccc gaaacgacag tgttttgcgt tgccagcaag tccttcaaaa
     5941 caccggaaac cctgctcaat gcacaggcag tcaaggcgtg gtatcgcggt gcggggttct
     6001 cggaatccga aacggcgtgc catttttgcg cggtgtctgc cgacactgcg gcagctgcgg
     6061 cttttggtat cgcggcggaa cgcgtgtttg cgatgtacga ctgggtgggc ggacgctatt
     6121 ccgtctggtc gcccgtcggt ttgcccgtga tggttgcggt cggcggggcg cgtttccgcg
     6181 agttgttggc gggggcgcac gcgatggaca gccatttttt ccacacgccg ccgcgccgca
     6241 acattcccgt tctgatggca ctgattgccg tgtggtataa caatttccaa cacgcggacg
     6301 ggcagaccgc cgttccgtac agccacaacc tgcgcctgct gccggtgtgg ctgaaccagc
     6361 tcgatatgga gagtttgggc aaaagccgcg cttcagacgg cagtcccgcc gtgtgcaaaa
     6421 cgggcggcat cgtgttcggc ggtgaagggg tcaactgcca gcacgcctat ttccaactgc
     6481 tccatcaagg cacgcgcctg attccctgcg actttatcgt cccgatgacg gcgcagggcg
     6541 tggaggacgg acgcagccgt tttaccgttg ccaacgcctt tgcccaagcg gaagccctga
     6601 tgaagggcaa aaccttggac gaagcacgcg ccgaactggc agatttgccc gaagcggaac
     6661 gcgaacgcct cgcgccgcac aaagagttcc ctggcaaccg ccccagcaac agcatcctgc
     6721 tcgaccgcct cacgccctac aatttgggta tgctgatggc ggcttacgaa cacaaaacct
     6781 tcgtccaagg cgcgatatgg aacgtcaacc ccttcgatca gtggggggtg gaatacggca
     6841 aacagttggc aaaaaccatc atcggcgaac tggaaggcgg cacgtccgta cacgatgcct
     6901 cgaccgaagg gctgatggcg ttttaccgcg aatgccgtct gaaaggcggc ggtgcggcat
     6961 aaaggtactg ccgcccgccc gattccgaaa cgccaatgtt cggcaaccgc ccgcgtattg
     7021 ctgacgaata tgcgtttgcg tggcacaata gcgcattcat ttcaaatgaa catactgctt
     7081 gaaaataccg gcaagcgtcc cacgaaacat ctcacataag gaaacattat gtctttgcaa
     7141 aacattatcg aaaccgcctt tgaaaaccgc gcggacatta atccgaccac cgttactccc
     7201 gaagttaaag aagccgtgtt ggaaaccatc cgccaactcg attccggcaa actgcgcgtc
     7261 gccgaacgct tgggcgtggg cgaatggaaa gttaacgaat gggcgaaaaa agccgtgttg
     7321 ctgtccttcc gcatccaaga caacgaaatc ctcaacgacg gtgtgaacaa atacttcgac
     7381 aaagtgccga ccaagtttgc cgactggtct gaagacgagt tcaaaaacgc aggcttccgc
     7441 gccgttccgg gtgcggttgc ccgacgcggc agctttgtgg cgaaaaatgt cgtgctgatg
     7501 ccgtcttacg tcaacatcgg cgcatacgtt gacgaaggtg cgatggtcga tacttgggcg
     7561 accgtcggct cttgcgcgca aatcggcaaa aacgtccact tgagcggcgg tgtcggcatc
     7621 ggcggcgtac tcgaacccct gcaagccagc ccaaccatta ttgaagacaa ttgcttcatc
     7681 ggtgcgcgtt ccgaaatcgt tgagggcgtg attgtcgaag aaggcagcgt gatttctatg
     7741 ggcgtgttca tcggtcaatc caccaaaatc ttcgaccgca ccaccggcga aatctaccaa
     7801 ggccgcgtac cggccggttc ggtcgtcgtg tccggcagta tgccttccaa agacggcagc
     7861 cacagccttt actgcgccgt catcgtcaaa cgcgtggacg cgcaaacccg tgcgaaaacc
     7921 agcgtgaacg aattgttgcg cggcatctga tgccttaaac cgtatttgaa acgtccgatg
     7981 ccgtctgaaa tccgcttcgg acggcattgc cgtttgcatg ctgcaacgtg aaaacacaga
     8041 aacagggaca atttgctata atcaacggtt tagaatgaac cgaacactat ttgaaggata
     8101 caaaatgggt tttctgcaag gcaaaaaaat tctgattacc ggcatgattt ccgagcgttc
     8161 catcgcttat ggcatcgcca aagcctgccg cgaacaaggc gcggaactgg cgtttaccta
     8221 cgttgtggac aaactggaag agcgcgtccg caaaatggcg gcggaattgg gttccgaact
     8281 cgtattccgc tgcgatgtcg ccagcgacga cgaaatcaac caagtgttcg ccgacttggg
     8341 caaacattgg gacggcttgg acggtttggt gcattccatc ggcttcgcgc cgaaagaagc
     8401 cttgagcggc gacttcctcg acagcatcag ccgcgaagcg ttcaacaccg cacacgaaat
     8461 ttccgcatac agcctgcccg cattggcaaa agccgcccgt ccgatgatgc gcggcagaaa
     8521 ctccgccatc gttgccctga gctacttggg cgcggtgcgc gcgattccaa attacaacgt
     8581 gatgggtatg gcaaaagcca gccttgaggc gggcatccgt tttaccgccg cctgtctggg
     8641 caaagaaggc atccgctgca acggcatttc cgccggcccg attaaaacgc ttgccgcctc
     8701 cggtatcgcc gatttcggca aacttttggg acacgtcgcc gcccacaatc cgctgcgccg
     8761 caacgttacc attgaagaag tcggcaatac cgccgccttc ctgctgtccg acctgtcgtc
     8821 cggcattacc ggcgaaatca cttacgttga cggcggttac agcattaatg ccttgagcac
     8881 cgagggataa tccgccgttt tcaaatccgt gcgccgtccg taccgcgtat cggtttcggg
     8941 cggcggtttg ccgtctgaag cgtatttcta gggaaatgcc cgacttgctg caggtgcagc
     9001 ggaaatggtc cgaacgcgaa aatgccgtct gaaacgccaa acgggtttca gacggcattt
     9061 ttttatttaa agcatcagca cacttcaacc agccagccgt atttgtcttc cgccaaacca
     9121 tactggatgt cggtaatcgc cttacggatg gcatagccgc gttcttggct tttcacttcg
     9181 atttctttgc cgccgatgac gaaggaagta acgggcgaga tgacggctgc cgtaccggtc
     9241 agaatcgcct ccgcaccgtt ttccaccgca gctttgagtt cgtcaaccgt gaaattgcgt
     9301 tcgctgacgg tatagcccaa atctttggca accgtcagta cggaatcgcg ggttacgccg
     9361 tgcaaaaatt cgtcggtcag cggtttggta atgatttcat cgccgttaat caggataaag
     9421 ttggacgcgc cggtttcctg cacgtcgccg ttcgggcaga agaggacttg gtttgcgccg
     9481 tattcggctt tcgccttcag cacccagtgc atggcggaag cgtagttgcc gccgcatttg
     9541 acgcggccca tatgcggggc gcagcggatg tgttcggttt ccactaaaat tttgacgggc
     9601 gagccgactt tgaaatagtc gccgacgggg gaagccaaaa tatacagcag ggcggtttcg
     9661 gaaggagaac cggccttgcc gatgacggga tcggtaccga ttaaggtcgg acgcaggtac
     9721 agggcggcag gcgcatcggg aatttcatcg gcggcgcgtt tgaccaattc gattagcgcg
     9781 tcgagatagg cttgggtttc ggggcgcggc aggtgcaaaa tgtccgcact ttgccgcata
     9841 cgcgcgatgt tggcagtcgg acggaacagt acgattttgc cgtctgcctg acggaaggct
     9901 ttcagtccct cgaaacattc gctgctgtag tgcagggcgt gcgcacccgg tgcgagggag
     9961 aggtcttggg aagattgcca ttcggtcggc tgccatttgc cttcgcggta ggcgaggacg
    10021 ggcatttgac tgtgaaaaac gctgccgaat acggcgggta cgggtctgct catgatgtaa
    10081 agcctttctt attctgatat gtttcaatga acggtttgaa tttgaagatt gtaaagatac
    10141 gcctgcaaac agggttttga caagtgcgcg gcgggttttt ctgtcgatgc ggtgtccaat
    10201 ccgttatttt tcaaatggaa aggaacggtg tatttggtaa aattgtcggc aatcgcatac
    10261 tccgtatgtc gtccgaacac gctgccgcat cctatccgaa accgtgcaaa tcgtttaaac
    10321 tagcgcaatc ttggttcaga gtgcgaagct gtccgggcgg cgtttttatt tacggagcaa
    10381 acatgaaact tatctatacc gtcatcaaaa tcattatcct gctgctcttc ctgctgcttg
    10441 ccgtcattaa tacggatgcc gttaccttct cctacctgcc cggacagaaa gtcgatttgc
    10501 cgctgattgt cgtattgttc ggcgcgtttg tcgtcggcat cgtgttcgga atgtttgcct
    10561 tgttcggacg gttgttgtcg ttacgtggcg agaacggcag gttgcgtgcc gaagtaaaga
    10621 aaaatgcgcg tttgacgggg aaagagctga ccgcaccacc ggcgcaaaat gcgcccgaat
    10681 ctgccaaaca gccttgagaa agccgatatg gacaatgaat tgtggattat cctgctgccg
    10741 attatccttt tgcccgtttt cttcgcgatg ggctggtttg ccgcccgtgt ggatatgaag
    10801 actgtattaa agcaggcaaa aagcataccg tcgggatttt ataaaagtct ggatgccttg
    10861 gttgaccgca acagcggacg tgccgccagg gaattggcag aggtcattga tcagcagcct
    10921 cagtcatacg acctaaacct caccctaggc aagctttacc gtcagcgtgg cgaaaacgac
    10981 aaagccatca acatacaccg gacaatgctc gattctcccg atacggtcgg cgaaaagcgc
    11041 gcgcgcgtcc tgtttgaatt ggcgcaaaac taccaaagtg cggggttggt cgatcgtgcc
    11101 gaacagattt ttttggggct gcaagacggt aaaatggcgc gtgaagccag acagcacctg
    11161 ctcaatatct accaacagga cagggattgg gaaaaagcgg ttgaaaccgc ccggctgctc
    11221 agccatgacg atcagaccta tcagtttgaa atcgcccagt tttattgcga acttgcccaa
    11281 gccgcgctgt tcaagtccaa tttcgatgtc gcgcgtttca atgtcggcaa ggcactcgaa
    11341 gccaacaaaa aatgcacccg tgccaacatg attttgggcg acatcgaaca ccgacaaggc
    11401 aatttccctg ccgccgtcga agcctatgcc gccatcgagc agcaaaacca tgcatacttg
    11461 agcatggtcg gcgagaagct ttacgaagcc tatgccgcgc agggaaaacc tgaagaaggc
    11521 ttgaaccgtc tgacaggata tatgcagacg tttcccgaac ttgacctgat caatgtcgtg
    11581 tacgagaaat ccctgctgct taagtgcgag aaagaagccg cgcaaaccgc cgtcgagctt
    11641 gtccgccgca agcccgacct caacggcgtg taccgcctgc ttggtttgaa actcagcgat
    11701 ttggatccgg cttggaaagc cgatgccgat atgatgcgtt cggttatcgg acggcagcta
    11761 cagcgcagcg tgatgtaccg gtgccgaaac tgccacttca aatcacaagt ctttttctgg
    11821 cactgccccg cctgcaacaa atggcagacg tttaccccga ataaaatcga agtttaacca
    11881 ccaccgaaag gaacacaaaa aatgcgctta ctccatacta tgctccgcgt gggcaatctc
    11941 gaaaaatccc tcgatttcta ccaaaacgtt ttgggtatga aactgctccg ccgaaaagat
    12001 tatcccgaag gcagatttac ccttgccttc gtcggttacg gcgatgaaac cgacagcacg
    12061 gttttggaac tgacgcacaa ctgggatacg gaacgatacg acttgggcaa cgcctacgga
    12121 cacatcgcgg ttgaagtgga cgatgcctac gaagcctgcg aacgtgtgaa gcggcagggc
    12181 ggaaacgtcg tccgcgaagc cggcccgatg aaacacggca caaccgtgat agccttcgtc
    12241 gaagaccccg acggatacaa aatcgagttc attcaaaaga aaagcggcga cgattcggtt
    12301 gcctatcaaa ctgcctgata ccgccgccgc caatgccgtc tgaagccttt aggggtttca
    12361 gacggcattt tgttgccgtc gacctgctgt ttgagcctgt gccggttcaa actttatccg
    12421 ttacaccgat aaggcaaaaa aagatgccgt ctgaaacggc atccttgatc tgcgaaaggg
    12481 cagttgggaa tcaaataccc aattcctgcg ccaatgcttg ggcacgtttg agtacgtcgc
    12541 cttccgcttc ttccagcaat ttctgcactg tctcggcagc ggcatcgcgg tcgccgattt
    12601 cgagatacat ttcggcaagg tcgtatttcg cttcggaagg cgcgtcagaa cctacagatt
    12661 ccgaagggaa accggtatct gcattatttg ggatattttc ttccgagagg tagatgctcc
    12721 aatctaccgt ttcctcctcg ccgtctttca ggaagtcggg caaagcgtct gcctcagagg
    12781 tgttggaatc aggcgtttcc aaagtgattt ccgttgcatt ttcctcaacg gccggtgctt
    12841 cagcaggttg caacagtgcg gacaaatcat cggcaacggt ttccgctgca ttttcctcaa
    12901 cggcaggtac ttcagaaggt tgaagtaatg cggacaaatc gtctgcggtg gcgttgaaat
    12961 cgggtgtttc ggcaacggtt tccgttacat tttcctcaac ggccggtgct tcagcaggtt
    13021 gcaacagtgc ggacaaatca tcggcaacgg tttccgctgc attttcctca acggcaggta
    13081 ctttagaagg ttgaagtaat gcggacaaat cgtctgcggt ggcgttgaag tcgggtgttt
    13141 cggcaacggt ttccgttata ttttcctcaa cggacggtgc ttcggcaggt tgaagcaatg
    13201 cggacaaatc gtctgcggcg gcgttgaaat cgggcgtttc aggcgcagtt tccgcgacgg
    13261 catcggtttc gtacactttc aggaaatcgt gcaactcttc cggtgtttgg acttcggcaa
    13321 ctgttttttc caagatggtt tcgggcgagg aagccttcag gaagcctgcc agtccggagg
    13381 gtgaggcagg ttttgcggaa gctgtttctt ctgtgccgat atggttgttt gagggcaggt
    13441 tgtcggagaa atcggtatcg acggtttccg gtttgttttc ggcagtttgg gcgacagatt
    13501 ccggttcggg cgtgtcgatg acgatttcga cagggttgta cgggttgaag gtctcgggct
    13561 cgtacacgct gtctgtggat tcgatggcgt tccaatcggc atccgcgcgt ttttgggttt
    13621 cttcatcctg cgtaagtgcg ccggataaaa tgccgttttg cgcggctgcc aggctgtcga
    13681 aatccaagtc gatgcggttg gaaggcgtat cggtttcgac atcgaacgtt tgttttgccg
    13741 ataactcttc ttcagattcc ccatctaagg caagtgtgtc gtttacatcg tttttcggag
    13801 cgggttcggg cgttgccgga gtttcgactt cggcaaaggt gatttctatg ccgtcgtctg
    13861 ccgcgtcgtc aaggtcaggc tcttcctcag ggacggattc ttcggtacgg cgcgcgcgtt
    13921 tggattgggc aaggcgcaaa agcagcagca gggcgattaa tgccgcgcct ccgccggcaa
    13981 gcagcaaggt gtacgaaccg ccgaacagac cgtcaaacag tccgctttcg gtttcttctt
    14041 cggcagaaac ctgttcgaca ggttcggaaa cggcgttacc ggtttcgtcg gtcggcgtgt
    14101 cgatggcaga agcggcggct tcttgggggg cggattcggc agcggtttcc gatgcggcag
    14161 tatttgcagc gggtacaggt tcgggtcgaa cggccggttt ttccgctttt gcttcgggcg
    14221 cggcaacttt tgcttcaggt ttttcaaccg gtttctctac cgttgcctgt ttggacggtt
    14281 cggacggcat ggatgcggtt tcggctttgg gtttcgccgt ttgcggtttg ggttgttccg
    14341 ctttgatcct gttcagattc ggaatgtgaa gcacgctgcc cgcacgcagt ctgccgtgtg
    14401 cggaaacatt tgggtttgcc ttcagcagcg catcggcaac ctgttcgagc gtcaggtgtt
    14461 tcgggcggat ggcggcggca atctgtttga ccgtttcgcc tttgcggacg gtatgggttt
    14521 tgccgttgta cgccggtttg acggctgcgt tcgcgctgtc ttttttatcg gttttgcgga
    14581 gggctttggc gttttgattt tcttgggact ctgctgtcgg agcggttttg cggtgtgtct
    14641 tgccgtctga aagtgcagat ttggttttgg gcgagtagcc gacaggatcg aggatggcgg
    14701 tgtattcgcg tacctgtgcg cctgcgccga tgcggaatac caggacggga tcgcggactg
    14761 cctgttcgga agaaacggca atgacggctt tgtcgcccaa cttgtggact ttggcggtca
    14821 gacctttttc ggaaacggta acgctgccgc cgcctagcag ggctttggct tcttcgccgg
    14881 ttacggtaat gctgccggaa aagggttcgt caaggttgga ctggatattc agtccgccca
    14941 gtccagcatg tgcctgaaag gatgcggcaa ctgcgacgga ggcggcaatc agtttgattt
    15001 gtctgttgtt tttcaagatg tatcccctgt gggttggcgg ctgaatacgg tttgaccgcg
    15061 tacagtctgt aaatttcgtc atcatcgggc atcggcgggg cagtcggccg gcgggcattt
    15121 aatatgtgaa tgtaccgacc gccgccacat tttaaacggc aatcattcgc cgtttttaca
    15181 aattatgaca tatctccatc ttttttcaaa aacatctgtg catatttgca tcaatcaaaa
    15241 caaaatttgt tggttttgca ggtgcaaaaa cagggttctg cctgtatgat tagcgtttat
    15301 ttgatttgct ttctcatttg gatatgaaat tcgtcagcga ccttttgtcc gtcatcctgt
    15361 ttttcgccac ctataccgtt accaaaaata tgattgccgc tacggcggtc gccttggttg
    15421 ccggtgtggt tcaggcggct tttctgtatt ggaaatataa aaagctggat acgatgcagt
    15481 gggtcggact ggtgctgatt gtgatattcg gtggcgcaac cattgttttg ggcgacagcc
    15541 gcttcattat gtggaagccg agcgttttgt tttggctggg cgcgctgttc ctgtggggca
    15601 gccacctcgc cggtaaaaac ggcttgaagg cgagtatcgg cagggagatt cagcttccgg
    15661 atgccgtatg ggcgaaattg acgtatatgt gggtcggttt cctgattttt atgggtatcg
    15721 ccaactggtt tgtgtttacc cggttcgagt cgcaatgggt caactataaa atgttcggct
    15781 cgactgcact gatgcttgtt ttctttatta ttcagggtat ttatctgagt acctgtctga
    15841 aaaaggagga ttgactgtgg aatattttat gttgctggca acagacgggg agaatgtgta
    15901 tgaagcgcgt atggcggcac gtcccgaaca cctcaaacgg ctggagacgc tgaagtcgga
    15961 aggccggctg ttgacggcag gcccgaatcc tttgccggag gactccaacc gcgtttcggg
    16021 cagtttgatt gtggcgcagt tcgagtcttt ggatgcggca gaggcgtggg cggaagacga
    16081 tccctatgtt catgcaggcg tgtacagcga agtgctgatc aagccgttta aagcggtgtt
    16141 caaataatgc cggccgtcga tttgatccgc gaacgcctgc agacgctcga tccgctggtg
    16201 ttggaaatcg gcgatgagag ccatctgcac aaaggacacg cgggcaatac cggcggcgga
    16261 cattatgccg ttttggtcgt tagcggccgt tttgaaggcg taagccgcct gaaccgccag
    16321 aaaacggtca aatcgctgct caaagatttg ttttcaggcg gcatgattca cgcgctcggc
    16381 atccgggcgg ctacccctga cgagtatttc catacggcgg actgaatgaa gtctgcccga
    16441 acatttcaat ttaaaattta aagagagaag attatgaaag caaaaatcct gacttccgtt
    16501 gcactgcttg cctgttccgg cagcctgttt gcccaaacgc tggcaaccgt caacggtcag
    16561 aaaatcgaca gttccgtcat cgatgcgcag gttgccgcat tccgtgcgga aaacagccgt
    16621 gccgaagaca cgccgcaact gcgccaatcc ctgctggaaa acgaagtggt caacaccgtg
    16681 gtcgcacagg aagtgaaacg cctgaaactc gaccggtcgg cagagtttaa aaatgcgctt
    16741 gccaaattgc gtgccgaagc gaaaaagtcg ggcgacgaca agaaaccgtc cttcaaaacc
    16801 gtttggcagg cggtaaaata tggcttgaac ggcgaggcat acgcgctgca tatcgccaaa
    16861 acccaaccgg tttccgagca ggaagtaaaa gccgcatatg acaatatcag cggtttttac
    16921 aaaggtacgc aggaagtcca gttgggcgaa atcctgaccg acaaggaaga aaatgcaaaa
    16981 aaagcggttg ccgacttgaa ggcgaaaaaa ggtttcgatg ccgtcttgaa acaatattcc
    17041 ctcaacgacc gtaccaaaca gaccggcgca ccggtcggat atgtgccgct gaaagatttg
    17101 gaacagggtg ttccgccgct ttatcaggca attaaggact tgaaaaaagg cgaatttacg
    17161 gcaacgccgc tgaaaaacgg cgatttctac ggcgtttatt atgtcaacga cagccgcgag
    17221 gtgaaagtgc cttcttttga tgaaatgaaa ggacagattg ccggcaacct tcaggcggaa
    17281 cggattgacc gtgccgtcgg tgcactgttg ggcaaggcaa acatcaaacc tgcaaaataa
    17341 ttctgaaaac gggatacggc ggcaagacgt tcagacaggc gttttgccgc cgcgcaggac
    17401 agggaatacc atgaaacaga aaaaaaccgc tgccgcagtt attgctgcaa tgttggcagg
    17461 ttttgcggca gccaaagcac ccgaaatcga cccggctttg gtggatacgc tggtggcgca
    17521 gatcatgcag caggcagacc ggcatgcgga gcagtcccaa aaaccggacg ggcaggcaat
    17581 ccgaaacgat gccgtccgtc ggctgcaaac tttggaagtt ttgaaaaaca gggcattgaa
    17641 ggaaggtttg gataaggata aggatgtcca aaaccgcttt aaaatcgccg aagcgtcttt
    17701 ttatgccgag gagtacgtcc gttttctgga acgttcggaa acggtttccg aaagcgcact
    17761 gcgtcaattt tatgagcggc aaatccgcat gatcaaattg cagcaggtca gcttcgcaac
    17821 cgaagaggag gcgcgtcagg cgcagcagct cctgctcaaa gggctgtctt ttgaagggct
    17881 gatgaagcgt tatccgaacg acgagcaggc ttttgacggt ttcattatgg cgcagcagct
    17941 tcccgagccg ctggcttcgc agtttgcagc gatgaatcgg ggcgacgtta cccgcgatcc
    18001 ggtcaaattg ggcgaacgct attatctgtt caaactcagc gaggtcggga aaaaccccga
    18061 cgcgcagcct ttcgagttgg tcagaaacca gttggaacaa ggtttgagac aggaaaaagc
    18121 ccgcttgaaa atcgatgcca ttttggaaga aaacggtgtc aaaccgtaat ggcatttcca
    18181 ataccgatgc cgtctgaagc cttttcagac ggcattgcac gttcaggtaa ggaggacggc
    18241 ttatgcgtgc ggtcatacag aaaacggtag gtgcaaaggt ggatgtcgtg tccgaaaacg
    18301 gcacggaaac ctgtggcaaa atcgacggcg gattcgtcgt gctgctcggc gtaacgcata
    18361 gcgacacaga aaaagatgcg cactatatcg ccgacaaaat cgcccatttg cgcgtgtttg
    18421 aagacgaagc gggcaagttg aacctgtctt tgaaagatgt cggcggcgcg gtgctgctgg
    18481 tgtcgcagtt tacactttat gccgatgcgg caagcgggcg gcggccttcg ttttcccaag
    18541 ccgcacctgc agaacaggcg cagcagcttt acctgcgaac ggcggaactg ttgcgcggac
    18601 acgggattca tgtcgaaaca gggcgtttcc gcacgcatat gcaggtgtcg ctctgcaacg
    18661 acgggccggt aaccatactg ctggactctt tcatgacgcg gatttcccca aaaatgaagg
    18721 ttgttccgga ttgaaattga atctgcaatg atgaaatatc gacaatgaac gacaatacac
    18781 acacccttcc cccgcgccgc ctttccgtcg ccccgatgct cgactggacg gacaggcact
    18841 accgttatat ggcgcgccag attacccgaa atgcttggct ttacagcgaa atggtcaatg
    18901 ccggtgcgat tgtttacggc gacaaagacc gctttttgat gttcaacgaa ggcgagcagc
    18961 ccgtcgccct gcaactgggc ggcagcgatc cgtccgattt ggcgaaagcc gccaaagccg
    19021 ccgaagcata cggctacaac gaggtcaacc tcaactgcgg ctgccccagt ccgcgcgtgc
    19081 agaaaggttc gttcggcgcg tgtctgatga acgaagtcgg gctggttgcc gactgcctca
    19141 acgccatgca ggacgcggtc ggtattcccg ttaccgtcaa acaccgcatt ggtgtggaca
    19201 ggcagaccga ataccaaacc gttgccgatt tcgtcggcac gttgcgcgac aaaaccgcct
    19261 gcaaaacctt catcgtccac gcccgcaacg cttggctgga cggtctttcc cccaaagaaa
    19321 accgcgacgt tcccccgttg aaatacgatt acgtttaccg cctcaagcag gagtttcccg
    19381 ggctggaaat catcatcaac ggcggcatca ccaccaacga agcaatcgcc ggacacctgc
    19441 aacacgttga cggcgtgatg gtcgggcgcg aggcgtacca caacccgatg gtgatgcgcg
    19501 aatgggacag attgttttac ggcgacactg cgaacccgat cggatacgcc gatttggtgc
    19561 aacgcctcta cacatacagc caagcccaaa tccaagccgg acgcggcaca atcctgcgcc
    19621 atatcgtccg ccacagcctc gggctgatgc acggcttgaa aggcgcgcgg acttggcggc
    19681 gtatgctttc cgacgcaacg ctcttgaaag acaacgacgg cagcctgatt ctcgaagcgt
    19741 ggaaagaggt cgaacgggca aatacgcgcg aatagggcgg ggctgtatgt gtgaaatgcc
    19801 gtctgaaggc ttcagacggc atttgtgcgt ttgtcgggcg gtgtttaggg ggtggtaatg
    19861 gcgtgtttcg gcactttgtc catatcccag tgtgccaccg cccagtcgag cagttcggca
    19921 gggcggtcgg cttccggtgc ttcgggcagg ttgaggtaac ggaacacttg gcggaggagt
    19981 tgttcgcggc ggtttaaatc caatgcgggt gcgagcgtct gtttcgacca tttctgccct
    20041 tgtgcgttgg tcagcagcgg caggtgggca tattgcggtg tcggaacgcc caaacattgc
    20101 tgcaaataga tttggcgcgg cgtagaaaca agcaggtctt gtccgcggac gatgtgggta
    20161 acgccctgtt cggcatcgtc ggcaacgacg gcgagctggt atgcccagta accgtctgca
    20221 cgaagcagga cgaaatcgcc gatgtcgcgg gcgaggtttt gggcgtaacc gccgacgatg
    20281 ccgtctgaaa agccgatgtc gcggtcgggg acgcggatgc gccacgcagg ctgtttgcct
    20341 tgcagtgcag ggcgttgctg cggattgcgg caacgtccgt tatagacgaa tccgtctgcg
    20401 ccgtggactg ccgcagcctg ccagtctttg cggctgcaat ggcagggata gaccagtccg
    20461 gcggttttca gacggcatag ggtttcttca tacagggcgt aacggcggct ctgataggcg
    20521 acttctccgt accactcgaa tccgaatgcc tcaagcgtgt gcaggatatg gcttgccgcc
    20581 cccggcattt cgcgcggcgg atcgaggtct tccatgcgaa ccagccacct tccgccgtgt
    20641 gcgcgtgcat cggcatagga agcgacggcg gtcagcagcg agccgatgtg gagcagcccg
    20701 gtcgggctgg gggcgaaacg tcctgtgtac atatccggta cggccccttt atttaagact
    20761 attaatcaaa gccattatct catctttatt cagttccatc ccgggttctt caagtaaggt
    20821 taaatcatat agggcattat attgctcttc ggtagctgaa ccatccataa ggccggacga
    20881 gaaaaaatcg aaggctctat ctgcgattct ctcattactt gcatttctac taaccagttt
    20941 catcaattct gtatatattg tatggggagg taccggatcc ttaaaaatca gatctgaata
    21001 aatttcattg ggtgaaatga tttcgagtgc ttttgctatg gttctatttc cttttgtgtt
    21061 ggcgggtaat gtcgtgcatt aacttcttgc ccattaatat ttttagggcg aatccttgat
    21121 atgccgcact gtgtccggtc aaaagggcga tgccgtctga aagcctttca gacggcatcg
    21181 gggatgaaaa gtttaaacca acggtgcgag cagtttttca aacgcttctt caaactgttt
    21241 caaaccgtct tcctgcaaac gcgttgccaa ggtttcgaca tcgatgccga gcgcggcggt
    21301 ttcggcgagc tgcgcttgtg cttcttctac gccttcggtc agcgtggctt tggctgtgcc
    21361 gtggtcgata aaggctttga gcgtggcatc gggaacggtg ttgacggtgt gcgcgccgat
    21421 caggctgtca acgtagagcg tgtcgggata ggctgggttt ttcacgccgg tagatgccca
    21481 taaaagctgc acgcggtttg cgccttgggc ttccagtgcg gcaaattcgg gggcggtgaa
    21541 atattgttcc caatcttgat aggcggcttt ggcaagggcg atggcgattt tgcctttgag
    21601 ccggtcgggc agtgttgtgt ccagcgcact gtccacacgc gagatgaaga agctggcgac
    21661 gacgtggata tgggcaacgc tttgtccggc tgccaagcgt ttggcaatgc cgcgcgcgta
    21721 ggcggcgtag gctttgaggg tttgggcgcg tgagaacagc agcgtgaggt ttacgctgat
    21781 gccgtctgaa acgagggttt cgagcgcatc gatgcctgcg tcggtggcag gcactttaat
    21841 catcgcgttt ttgcacccga tggcggcgta gaggcggcgc gcttcttcaa ccgtgccttg
    21901 cgcgtctttg gacaattcgg gcgaaacttc gaggctgacg aagccggttt tgccgccggt
    21961 ggattcgtgt tcggcaagac agacgccgca ggcggcacgc acatcggcaa ccgccatcgt
    22021 ttcgtagcgt tgtttggggg tcaggtcttg ctgcttgagg gcggcaacct cgtcggcgta
    22081 aagcgcatcg ccggcgaagg ctttttgaaa gatggcggga ttggaagtta cgccgcacac
    22141 gccttgtttc agcatttgcg ccaattcgcc gctttgcacg agcgagcggg aaaggttgtc
    22201 cagccagatt tgttgtccta atgctttaac gtccgataaa atagtcattt ctgattcctt
    22261 tggatggatg tgtatgtttc aaaggcttat gctaccccga ttcggaaatt ttgggtagtt
    22321 ttattacagc aaaggcggat ggcgatggca ggaaacgaaa aatatcttga ttgggcacgc
    22381 gaagtgttgc acaccgaagc ggaaggcttg cgcgaaattg cggcggattt ggacgaaaac
    22441 ttcgcccttg cggcggacgc gttgttgcac tgcaaaggca gggtcgttat cacgggcatg
    22501 ggcaagtcgg gacatatcgg gcgcaaaatg gcggcaacca tggcctcgac cggcacgccc
    22561 gcgtttttcg tccaccctgc ggaagcggca cacggcgatt tgggcatgat tgtggacaac
    22621 gacgtggtcg tcgcgatttc caattccggt gaaagcgacg aaatcgccgc catcatcccc
    22681 gcgctcaaac gcaaagatat cacgcttgtc tgcatcaccg cccgccccga ttcaaccatg
    22741 gcgcgccatg ccgacatcca catcacggcg tcggtttcca aagaagcctg cccgctgggg
    22801 cttgccccga ccaccagcac caccgccgtt atggctttgg gcgatgcgtt ggcggttgtc
    22861 ctgctgcgcg cccgcgcgtt cacgcccgac gacttcgcct tgagccaccc tgccggcagc
    22921 ctcggcaaac gcctactttt gcgcgttgcc gacattatgc acaaaggcgg cggcctgcct
    22981 gccgtccgac tcggcacgcc cttgaaagaa gccatcgtca gcatgagtga aaaagggctg
    23041 ggcatgttgg cggtaacgga cgggcaaggc cgtctgaaag gcgtattcac cgacggcgat
    23101 ttgcgccgcc tgtttcaaga atgcgacaat tttaccggtc tttcgataga cgaagtcatg
    23161 catacgcatc ctaaaaccat ctccgccgaa cgtctcgcca ccgaagccct gaaagtcatg
    23221 caggcaaacc atgtgaacgg gcttctggtt accgatgcag atggcgtgct gatcggcgcg
    23281 ctgaatatgc acgacctttt ggcggcgcgg
//
LOCUS       DAXHTR010000011        22374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.011, whole genome shotgun sequence.
ACCESSION   DAXHTR010000011 DAXHTR010000000
VERSION     DAXHTR010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.011"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            1..100
                     /locus_tag="ACFNML_000320"
                     /pseudo
     CDS             1..100
                     /locus_tag="ACFNML_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(121..1035)
                     /locus_tag="ACFNML_000321"
     CDS             complement(121..1035)
                     /locus_tag="ACFNML_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6391771.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEHFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1291..2760
                     /locus_tag="ACFNML_000322"
     CDS             1291..2760
                     /locus_tag="ACFNML_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HFC6391772.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWAGGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3012..3383
                     /locus_tag="ACFNML_000323"
     CDS             3012..3383
                     /locus_tag="ACFNML_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391773.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLDSSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3456..4865
                     /gene="thrC"
                     /locus_tag="ACFNML_000324"
     CDS             3456..4865
                     /gene="thrC"
                     /locus_tag="ACFNML_000324"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFC6391774.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAVFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFKATQSNDEQVSFCVPSGNFGNVCAGHIAKQMGLPIR
                     RLIVATNENDVLDEFFKTGAYRPRNSEHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALDKVGGKYGFTSGKSTHADRLATIKQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDVAIPRPAVLKGLETLPQRV
                     QTAPNSADAVKGIIEQTLA"
     gene            4912..5706
                     /locus_tag="ACFNML_000325"
     CDS             4912..5706
                     /locus_tag="ACFNML_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391775.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAIATESKRLYELRSVGIAVPELLAVRK
                     NALMFGNLAGIPLDAQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMWDG
                     KNISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSEQP
                     AEIQRLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            5894..6670
                     /locus_tag="ACFNML_000326"
     CDS             5894..6670
                     /locus_tag="ACFNML_000326"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6391776.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            6746..6852
                     /locus_tag="ACFNML_000327"
                     /pseudo
     CDS             6746..6852
                     /locus_tag="ACFNML_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7441..9912)
                     /locus_tag="ACFNML_000328"
     CDS             complement(7441..9912)
                     /locus_tag="ACFNML_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HFC6391777.1"
                     /translation="MKKTCFHCGLDVPEHLHLTVRYENEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLRTVAHLLDQARRTRHIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMQSEKMPSEQ"
     gene            complement(9909..11087)
                     /gene="hflX"
                     /locus_tag="ACFNML_000329"
     CDS             complement(9909..11087)
                     /gene="hflX"
                     /locus_tag="ACFNML_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HFC6391778.1"
                     /translation="MTGRTGGNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLQEIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(11092..12372)
                     /locus_tag="ACFNML_000330"
     CDS             complement(11092..12372)
                     /locus_tag="ACFNML_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HFC6391779.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHASYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNSRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGSGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            12374..12502
                     /locus_tag="ACFNML_000331"
     CDS             12374..12502
                     /locus_tag="ACFNML_000331"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391780.1"
                     /translation="MPSSRRGNGVFYQNGKLANAVSDCRLPNRQTFPRADAEPDAV"
     gene            complement(12525..13250)
                     /locus_tag="ACFNML_000332"
     CDS             complement(12525..13250)
                     /locus_tag="ACFNML_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HFC6391781.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13323..14000)
                     /gene="radC"
                     /locus_tag="ACFNML_000333"
     CDS             complement(13323..14000)
                     /gene="radC"
                     /locus_tag="ACFNML_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFC6391782.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            14027..14149
                     /locus_tag="ACFNML_000334"
     CDS             14027..14149
                     /locus_tag="ACFNML_000334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391783.1"
                     /translation="MPSETDVPTRQISEMPSESDFRFRRHLYFKTVLRRFFQLD"
     gene            complement(14123..15472)
                     /gene="gshA"
                     /locus_tag="ACFNML_000335"
     CDS             complement(14123..15472)
                     /gene="gshA"
                     /locus_tag="ACFNML_000335"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFC6391784.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWYSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15732..17141
                     /gene="leuC"
                     /locus_tag="ACFNML_000336"
     CDS             15732..17141
                     /gene="leuC"
                     /locus_tag="ACFNML_000336"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFC6391785.1"
                     /translation="MTAQTLYDKLWNSHVVREEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17238..17492
                     /locus_tag="ACFNML_000337"
     CDS             17238..17492
                     /locus_tag="ACFNML_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391786.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17554..18195
                     /gene="leuD"
                     /locus_tag="ACFNML_000338"
     CDS             17554..18195
                     /gene="leuD"
                     /locus_tag="ACFNML_000338"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFC6391787.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYRLSIDLAGQTLT
                     TPGGETFAFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18364..19635
                     /locus_tag="ACFNML_000339"
     CDS             18364..19635
                     /locus_tag="ACFNML_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6391788.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19651..20382
                     /locus_tag="ACFNML_000340"
     CDS             19651..20382
                     /locus_tag="ACFNML_000340"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HFC6391789.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20416..21486
                     /gene="leuB"
                     /locus_tag="ACFNML_000341"
     CDS             20416..21486
                     /gene="leuB"
                     /locus_tag="ACFNML_000341"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFC6391790.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21649..22212
                     /locus_tag="ACFNML_000342"
     CDS             21649..22212
                     /locus_tag="ACFNML_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFC6391791.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSTTIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5407 a         5865 c         5816 g         5286 t
ORIGIN      
        1 cggcatttcc aaagggcatt ttaagccgta tttgagagaa tgcggatggc gttttcagtg
       61 ttttcagcgt gataaaattc aaatttctat tttaaaacaa tcagcaaagc aggatttatt
      121 tcagttgtcc gagacaacgg gcggctgcac cgcctgcact ccccatactt tttcaatgca
      181 ctcgagcagg agtgagacgt ggggattatc ctcgtattct tccgcataaa tgaaattgag
      241 cggcatactg tggcggcacg attcgataat agccacgccc gcgccttctt ttgccgccgc
      301 ttcggctttg tttcccggca ccattgccac acctataccg cctgcaacca aatcgataat
      361 ggtttggggg tagtcgcaca agatctgttt tttgggtgag agccggttgc tgcgccaaaa
      421 ctggtgcagg tgcttcctac ttccggacac gcccgacatt tctatccata cgcattcttg
      481 caggctctgc ggaagggagc gggtcagatg gggatattgg ctttgcgggc aaatcagcgc
      541 gtaggtcagg ttttgcagga atacgctgcg gatgccgcgt tgggcggcgt tgccgagtat
      601 aaagccgccg tgcagggttt tgtgttggat gcgcgagagg atttcgccgc tcattccgta
      661 ttggatgtgc aggcgcgttt tgggggctgt ttgaccgata ttgtccgtca gcgcgacgag
      721 ttttgccgaa tcgatggggt ggataatgcc caaactggtc tcttccgaat aatcgcctgc
      781 cagcgttttg gcaaaatgct ccagcttgtg tttgtattgc agcagggatt ccgcttcggg
      841 caacagtatt tcgcccgccc gcgtcaatac catgcctttc cccgtgcgcc tgaacagcgg
      901 cgtgccgaca tattcttcaa gggctttaat ttgggcagaa acggcaggct gggaaaggaa
      961 aagtcgtttg gcggcttggg taaggttgcc ctcgtgcgcg acggcgacaa atgattttaa
     1021 ttgtacggca tccatatatc cctccttgtg cggatgtttt ctatatttgt gcaatcgaaa
     1081 tcttttaggt ggattgttgc tgaaaattaa ctttttaatc aagtggtttg taaattgtat
     1141 cagttttccg gatgatggtt atcaaaaaaa aagattggtt ttattgcctg tttgggcttt
     1201 aaatggggtt acggcttccg aacgcagccc gtatcaaaaa gaaaagtcat gcgccccttt
     1261 tacgaggcgc gatatataag gaggaaggtt atggaaaaac ataatgggac ttatcgggat
     1321 ttgcaccgtc cggcttcgga atttgcgacg cgggacgaat atttggaaca tgaattgcag
     1381 attatgcaac caaaacgctg gcggcccaac ctgccctttc gcgattaccg cttcgagtgg
     1441 gaggatttga ttcctgcgat ggcgggaacg attggaaaag tggtgatggt gggggcggtg
     1501 gcggcggcgt ttgccgcacc tttggggctg cctgacagct ttgtactgga aaatgtgcgc
     1561 tatgagcttt taatcgccgc cgcgtttatc ttattggtat cgggcttttt tctgcccggc
     1621 gccaacctgc ccggtacgca cgggccgctg attccgatga ttcccatcgt tgtgtcggca
     1681 ggcgggcatc ctttggcgtt cggcatttcg attgcggttt taggtctgct gatggcttta
     1741 tttcgcggcg gcagtattat ggcgaagctg acaagcaacg gcgtatgcgg cggattatta
     1801 ctctatttgg gctttatcgg cacgacgggg caggtaaaaa aattgttttc gtgggcaggc
     1861 ggttttaata tgccctacat cgcttttacc gtcattattg taacgattgt gatgtacgct
     1921 ttgttggagc attggaaaaa acgctggtta gccgtgcctt tgggatgctt gattgccggt
     1981 gtggtggcat ttgcattggg tgcgccgttt gagtttcaca ccgcccccgg cctgcctcca
     2041 atgagtcctg cttattggtg gggtgaaaac agcggctggc atctggggtt gccgacggca
     2101 gaaagttttt tggttgtctt tccatttgcg gtattggctg ttgcaatgtg gtcgcccgat
     2161 tttttaggac atcaagtgtt ccaaaaaatc agctatccgg aaaaaaccga taaggtattg
     2221 atgaatatag acgacaccat gacaagttgt tctgtccgtc aagcagtggg ttctatttta
     2281 gggggtgcaa attttacctc ttcttgggga acttatatcg taccggcatc gattgccaaa
     2341 cgccccattc cgggcggtgc ggttttaacg gcggttttat gtattatcgc cgggttgtgg
     2401 ggctatccga tggacttggc gatttggcag ccggtattga gcgtagcctt ggtcgtaggc
     2461 gtatacttac cgcttttgga agcgggcatg gaaatgacgc gcaaaggcaa aaccacccaa
     2521 tccgccgcca tcgtggtgtt ctcttccgcc ttggtcaatc cggttttcgg ctgggcgttg
     2581 acgatgctgt tggataattt gggcttaatc ggctgcaaag agcgcagtgc gcaattaggt
     2641 ttcgccggac gcgtgttgat acccgcagta ggtttcttga tcttgtgtgt ggcgatgggt
     2701 gcggtcggga tgctgcccgg tatcccgccg tttttggaac acttcaaatc tttgggctag
     2761 gctgaaatcg gaaatgccgt ctgaaccgct ttcagacggc atttttgcaa acaggcaaaa
     2821 tgacggcggc gggatttttt attttcccga ttgaagtata atgttgccgg gcttcaaccg
     2881 gatattcaaa acggtttgtt ccaacactcg gaacggcgca taaaacgccg cctttcgcgt
     2941 tatcccgaac ggggcggcta atcagattct atcgccataa aaggcggggt ttcaaccgaa
     3001 aaggaattga gatgaataaa accttgtcta ttttgccggt ggcaatctta ctcggcggct
     3061 gcgccgccgg aggcggtaac acattcggca gcttagacgg tggcacaggc atgggcggca
     3121 gcatcgtcaa aatggcggtc gggagccaat gccgtgcgga attggacaaa cgcagcgaat
     3181 ggcgtttgac cgcgctggcg atgagtgctg aaaaacaggc ggaatgggaa aacaagattt
     3241 gcgcttgcgt cgcccaagaa gcacccgaaa ggatgaccgg caacgatgtg atgcagatgc
     3301 tggattcgtc cacgcgcaat caggcacttg ccgccctgac cgccaaaacg gtttccgcct
     3361 gcttcaaaca cctgtaccgc taaccttctt cagacggcat gaggcagatg ccgtctgaag
     3421 ccgttttcct gcaatttcga cagacgagaa aaatcatgaa atacatcagc acgcgcggcg
     3481 aaaccgcaca caaaccgttc agcgaagttt tattgatggg gcttgcaccc gacggcggcc
     3541 tgatgctgcc ggaacattat ccgcaaatcg ggcgcgaaac cttggacaaa tggcgcggtt
     3601 tagcttatcc cgaattggcg tttgaaatta tgcgcctgtt tgttacggat attccggagg
     3661 acgatttgcg cgacattctg aaccgtactt acacggaagc ggcgttcggt actaaggaaa
     3721 tcacccccgt ccgcacgctt tcagacggca tcaaaatcca agccttgtcc aacggcccga
     3781 cgctggcgtt caaagatatg gcaatgcagt ttttgggcaa tgcgtttgaa tatgttttaa
     3841 acaaagaagg caaaaaactc aatatcttgg gcgcaaccag cggcgatacg ggttcggctg
     3901 cggaatatgc cttgcgcggc aaaaaaggtg tgaatgtatt tatgctgtcg cccgatggca
     3961 aaatgagcgc gttccaacgc gcgcaaatgt acagcctgca agacgggaat atccacaata
     4021 tcgccgtgaa gggaatgttt gacgactgtc aggatattgt gaaggcggtg cagaacgatg
     4081 ccgtgttcaa ggaaaaatac catatcggta cggtcaactc gattaactgg ggacgcatcg
     4141 tcgcgcaagt ggtttattac tttgccggct atttcaaagc gacgcaaagc aatgacgagc
     4201 aagtcagctt ttgcgtgccg agcggcaact tcggcaacgt ttgcgcggga cacatcgcca
     4261 aacaaatggg cttgcccatc cgccgcctga ttgtcgcgac caatgaaaac gatgtgttgg
     4321 acgagttttt caaaaccggc gcataccgcc cgcgcaacag cgagcatact tatgttacct
     4381 ccagcccgtc gatggacatt tccaaagcgt ccaacttcga gcgtttcgtg ttcgacctga
     4441 tggatcgcga tcctcaggaa atcaatacgc tgtgggcgga agtggctgca ggcaaaggct
     4501 ttgacctgcg atttgccttg gacaaagtcg gtggcaaata cggctttact tccggcaagt
     4561 ccacccacgc cgaccgcctc gccaccatca aacaggttta cgagcaagac caagaactca
     4621 tcgacccgca cactgccgac ggcgtaaaag ttgcccgcga agtgcgcgaa gaaggtgaaa
     4681 cggtggtttg tttagaaact gcgttggcag cgaaattcga tgcaaccata cgcgaagccg
     4741 tcggcgatgt cgccattccg cgccccgccg tgctgaaagg tttggaaaca ttgccgcagc
     4801 gcgtgcaaac cgcgccgaat agtgcggatg cggtaaaagg catcatcgaa caaacccttg
     4861 cctgatgtcc cgtgtgggcg gcattccgtc cgaacttaag attgaaagaa aatgacctta
     4921 cctatgcagg aaacccgttt ttctatactg ctggacgaac tggcggcaaa acaggaggca
     4981 gccatcgctc cccacctgct tgccgacgga accaaggtat gggtacgcaa agcaggcagg
     5041 cacaatgcgc ggtggcgtta cgcgctgctc ggtatggttg cccgatattt gaaactgggg
     5101 gtgttgaagc cggttcccag cctcggcggc gagcctgcca ttgcaaccga atcaaaacgc
     5161 ctgtacgaat tgcgctcggt ggggatagcc gttcccgaat tgctcgccgt caggaaaaat
     5221 gcccttatgt tcggcaattt ggcaggcatc ccgctcgatg cgcaaatccg ccaagaagcc
     5281 gaagccggaa aggcggacgc ttggcttgcc ggtttggaag ccattgcacg cgtgcataaa
     5341 aaacagcagt tcctcagtca ggcgtttgca cgaaatatga tgtgggacgg aaaaaacatc
     5401 agctttctag attttgaaga cgacccttcc gaagtcctga ccatagcgca atgccaagcc
     5461 cgcgattggc tctgctacat ccattcgacc gcgctgatat tgaaaaacgg cggactgctg
     5521 gaagcggctg cggaaaaatg gggcggcgta ctgtctgagc agcctgccga aatacaaagg
     5581 ctgattgccg ccaccgtcaa accgatcctc ccgatacgca ggctggaaca cccccgctgg
     5641 ggacgggacg cgctcaggct ggcagcctcg atttccctga tttcccttgc cgatataccg
     5701 ccataagccg ccgcccgccc cgcgatgccg tctgaagccc ttcagacggc atataaccaa
     5761 cgcgcatcaa gcattgtcgc aaccatccgt ttcgtttaga attttgactt tatcccgaat
     5821 cgggcagaca ttttcactct gccgcacatc ccgaacaagc actatccatt tcttttactg
     5881 acaggaaacc caaatggcag cattcaatac ccaaaaagta ttgtccgtac accactggac
     5941 agacgcatat tttaccttta cctgcacccg cgacgaatcg ttgcgctttg aaaacggaca
     6001 gttcgttatg gtcggattga tggtggacgg caagccgctg atgcgcgcat acagcgtcgc
     6061 ctccgccaac tgggaagaac acctcgaatt tttcagcatt aaagtccaag acggtccgct
     6121 gaccagccgc ctgcaacacc tcaaagtcgg cgacgacgtg ttaatcagca aaaaaccgac
     6181 cggaaccctg gttgccggcg acctgaatcc gggcaaacac ctttacttgt tgagcaccgg
     6241 taccggcatc gctcctttct tgagcatcac caaagacccc gaaatttacg agcaatttga
     6301 aaaaatcatc ctcgtacacg gcgtgcgcta caaaaaagat ttggcgtact acgaccgctt
     6361 taccaaagaa ttgcccgaac acgaatacct cggtgacttg gttaaagaaa aactgattta
     6421 ctacccgatt gtttcgcgcg aagaattcga acaccacggt cgcctaaccg acctgatggt
     6481 gagcggcaaa ctgtttgaag acatcggcct gcccaaaatc aacccgcaag acgaccgcgc
     6541 catgctgtgc ggcagccccg cgatgctgaa agatacctgc aaagttttgg acgatttcgg
     6601 actgaccgtc tctccgaaaa caggcgtacg cggcgattac ctgattgagc gtgcgtttgt
     6661 agatcaataa gctgtttgaa aatcccgtct gaacggcacc agcaatcaaa aaccccgaat
     6721 cctgccaatc aggattcggg gtttttatag tgaattaaat ttaaatcagg acaaggcggc
     6781 gagccgcaga cagtacaaat agtacggcaa ggcgagccaa cactgtaccg gtttaaattt
     6841 aattcactat atagtgggta aaaattagaa atgcacattt ccgtcattcc aacacaggtg
     6901 ggaatccgga cttacctgtt tcattatcgt ttgaaaataa aagagaaacc aaacatatag
     6961 attcccgctt gcgcgggaat gacgacggtg ttttgccgta gccaaagcag aaaaacatcc
     7021 ctattgtcat ttcatcatga tatgacaata ggatgttaaa aattaattat cattatatga
     7081 ttgatatata agaaaaactg tcccaataag aaatgttatg cagaaaataa aatgaaaagg
     7141 atggttttca ttgaaaatgg ttgttctgcc accgtcatac acagagccag tatttggaaa
     7201 aaaatgatcc atattgacat cattattcca tttgtcgcat aaatccactt tttaattttt
     7261 tccacattaa tataatctct tatttaataa aatcaaaata aaaatcaaat gcctaacaat
     7321 tcgatctcaa attttgaacg ccaacgtgtt tgagcggtaa caatacatac tttaagtttg
     7381 caattcttca tcgtcttatt tttgttttca tcaactatac gtctaaagca acgccgtccg
     7441 tcattgttcg gacggcattt tttcagactg cattttcccc cgtttgtgaa ggcgcagggc
     7501 gttgcccaaa accgccagcg aactgaagct catacccagt gcggctatcc acggttggac
     7561 atagcccaaa acggcaagcg gtacggcaat gatattgtac gcgcccgccc atatcaggtt
     7621 ttgccggata atatggcggg tgcgccgcgc ctgatcgagc aggtgggcga cggtacgcaa
     7681 atcttcgttc aataacacaa tgtccgcgcc gtccctcgca atatccgtcc cgcccgctgc
     7741 ggcggcggat acgtctgcct gcgccaaaac gggcgcgtcg ttgatgccgt cgcctatcat
     7801 cagcactttt ttcccttctt tttgcaaggc tttgacgtat tccagtttgt cctcgggcat
     7861 ggcttgggcg cggtagtgcg cgacacccag ggcgcgtgcg gtttcggcaa cggcggtttc
     7921 gcggtcgccg ctgaggatgt gcagggtcag gtttttgcct gccaactgcc gcaccgcctc
     7981 cgccgcgctg tctttcaagg ggtcggtcag gtagaacacg gcttggaaac cgctttgact
     8041 gccgaggtaa accgcgctgc cgccgccttc tgtttgcggt tctttacctg aaatttcggc
     8101 gacataggat gccctgccca atgcccaaac ctgtgtttcg ccgttgacgg tcagttgcgc
     8161 gcccacgcct tcgccgatgc ggttgaggcg ttgtttaata gcgatgtcgg ggacgctgcc
     8221 gtctgaaatg cggcagttga ggatggcgcg ggcaagggga tgttcggact gttgttctaa
     8281 agcctgcgcc accgcgagaa caaaggcttc gtctgtgcct ctcaacaatg agatacggcg
     8341 gacggcgggt ttgccttggg tcagcgtgcc ggttttgtcg aagatgatgt cggtggtttg
     8401 ggcgagggtt tcgattgcct gctttccgcc gattaaaata ccttcgcgcg ccagcgtacc
     8461 ggtagaagct gccagcgcgg tcggcgtggc aagcgataag gcgcaggggc aggtaatgac
     8521 cagcagggcg acggtaatcc acaatgcggt gtgcgcgtcg gcgtacagcg tccagccgat
     8581 gaagacgggg acggcaagca ggagttcgcc gaatatgaaa gacgaggcgt attgttccgc
     8641 caactcggca gtgcgcggtt tttgcgctaa ggcgcggtcg agcaggcgga cgatgtgcga
     8701 cagtcgcgtg ccaccgccgg tgcggtcggt gcgtataatc agggggcttt gcgtgttgag
     8761 tgtgccggcg gttacttttt cagacggcat tttggcgacg ggcaggctct cgccggtcag
     8821 catagattcg ttgacggcac tgcttccttc cagcaccgtg ccgtcaacgg ggatggtttc
     8881 gcccggtttg accagcacga tatcgcccgc cttcaatttg acgacagctg cctcgcaggt
     8941 ttcctgcgta tcggggtaat cgggcatatg atggcaaaac gcaggaatca gcttcaccag
     9001 cctctcggcg gcatcgcctg ccttacggcg ggcaatgtgt tccataaagc gtccgcccag
     9061 caggaaaaac agcagcatcg cgatggattc gaaatacatc ccctgccccg catttgtcgc
     9121 aaggctgtaa acgccggcga taaaggtcat gatgatggcg acggtaatcg gcgtatccat
     9181 gccgacgcgg cggtttttca agtcgcgcag cgcgccttga taaaacggga cggcgcaata
     9241 gaatacgacg ggcagcacca ttaaaaagcc gccccaatgg aggatttgca ggaaatcggg
     9301 ttcgatgtcg ccgccgtaaa ggtaggtcgg cagcgcgaac atcatcgtct gcatcatccc
     9361 cagcccggca acggcgaggc ggacgatgta ttgtttgcgt tctttttggt tggcggcttc
     9421 gattttttgc gcgtcatagg gtgcggcggt gtagcctatc tgcctgattt tcaacagaat
     9481 gtcggaaagg cggattttgc cgtcgtccca gacgacgcgg cagcggtgcg tgctgtaatt
     9541 gaggtcgatg cggacgatgc cgtctgtacg caaaagctgc tgttcgatca gccagacgca
     9601 ggcggcgcag gtgatgccgc cgagcattaa aaccgcctcg cgcgtgccgc cgtgggtttc
     9661 cacaaagtcg gactggactt cgggcaggtc gtacaggcgg atttggtcga ggatttcttg
     9721 gggcggcagc tcggtttttt gcgcgtcggc ggtgcgttgt ttgtaataac tgcccaagcc
     9781 cgcgtcaata atgctttgtg cgaccgcctg acagccggcg cagcaggttt cgcggtcttc
     9841 gttttcgtaa cggacagtca ggtggaggtg ttcgggaaca tccagaccgc agtggaaaca
     9901 ggtttttttc atggcatttc ggtttcgtct gtgtttggtg cggcggcaca agactcggca
     9961 atggcttcgc gcagtgcgtc tataccggta ttttcagcaa cggaaatgcg gacggcggca
    10021 atttttcccg cagcgtcgcg ccatatgccc gtgttttgtt cttcagacgg cagcaggtcg
    10081 gttttgttgt acaccttgat gcacggaata tcgccggcat ggatttcttg cagtacgttt
    10141 tccacgtctt caatctgctg tccgctgttc ggagcggcgg catcgacgac gtgcagcagc
    10201 acatcggctt gcgcggtttc ttccagcgtg gcggaaaagg cggaaatcag tttgtgcggc
    10261 agatcgctga cgaatccgac ggtatcggtc aggataatgc tgcattcggg actgatgtac
    10321 agccgccgcg ccgtcgtgtc gagtgtggcg aaaagctggt ctttcgcata tatgcccgac
    10381 ttggtcagcc ggttgaacag gctggatttg ccgacattgg tatagccgac cagcgcaaac
    10441 gttttgattg tgcccgattc gcgggacttg cggcgcaggg cgcgctgttt tttgaggttg
    10501 gcaagctgtt ttttcaaggc attgatccga tgggcgatca atcggcggtc ggtttccagt
    10561 ttggtttcgc cggggccttt catgccgata ccgccgcgct ggctctgcag atggccgtaa
    10621 ccgcgtatca agcgtcccgc caaatggctc aattgcgcca actcgacttg cagcctgcct
    10681 tcctgcgtgc gggcgcggcg agcgaaaatc gccagaatca gccctaccct gtccaatacg
    10741 cggcatttca gttctttttc aaggttgcgt tcctgcgtgg gcgtgagttc gtggttgaat
    10801 acgaccaaat cgatgccgtc tgcggcaact gcttctgaca gctccgccgc cttgcccgtg
    10861 ccgacaaaca gcgcggtgtg cggacggtcg cgtttggcag tctccacgcg cacggaatcg
    10921 ccgcccgccg ctttgaccag ctcgacagct tccgccaatg ccgtctgaaa accgttcaga
    10981 cgggcggcac tgctgcccgt accatctttg tccaacatta cgcccaccag catgacgcgt
    11041 tcgggttgcg cttgggtact gccgttgccg cctgttctgc ctgtcatgta ttcaatcctg
    11101 taaataaagg gttacgcctc gttccgtttc tccttccggt gcaagcgtga tgcggctgcc
    11161 cgccattccg tcaacgctga agacgatgcc gcccgcctcg tgccttccta tggacaggcg
    11221 gctgccgggt cgctgccggt aggtataacg ggtacacgct tccaaacacc agcgtccgcg
    11281 ccagtggatg aaatattttt cgccttttct aaccgcaagt gtctcgccgc cgtttacctc
    11341 aaaataaggg taatcgggtt ttcgggttct aaactgccat ttcgcctgcg cgcgccgtcc
    11401 gttgcggaca tagtcgaata ccgccgtata aagcgtgccg tattccaaag gcttgagcgg
    11461 aaaaagcgcg aattggtacg cggtcagcct gccgttgggg tcgttgccgg cggttaaaac
    11521 cctgacgggg cggatttcgt ttttaccctg atacagcttg aaacttttca tcgtaatttt
    11581 gcctgccgcc tcggaaaaat caatgctggc aggattgccc gtgatttcat attccggcac
    11641 ggggtctgga cgctcgccgt ggaaataagg cagtgcgccg ctgccgacgg gataggctgt
    11701 atagagcagc tcctgtgcgg gcatggcttc gtcggtgtac acgaccgcac cgttatggca
    11761 ggcgttgcgg taatatttcc gtcctgcttc cggctgattt ctgccttggg cgcaatgccg
    11821 ctcaaacctg ctgttgccct gattgaatac gagaacggtt ttaccgtttt cgcgcacaaa
    11881 tgccgctcct gcctcatccg tatggcggtc aagtagggaa aggcggtggt agattgcgct
    11941 cattaatccg tccacttggc gttgctgcgt gcggatgtcg ctgtcggacg attcggcggc
    12001 ttcctcttcc gtgctgatgt tttcatgcac gccgttgtag agatacccgg caaggcgtgt
    12061 gcgttcggtc agcttttgtg cggtgtagtg cggattgtcg ggatggtgtt cgccgtgtcc
    12121 gtcttcggga ttgagcgtga ggtagcttgc gtgcctgcgg gcggagtttt ccaaaaccgg
    12181 cgcgtgtgcc agcttgtgca aaccgatttg ggctcggatg cggttgaggt agtgcagcgc
    12241 atcgccgccg tccctgattt gcggtgcgga cggatagaca agttcgcccg cgggcaggga
    12301 ttggttttgg gtatggtaga aaacgcctgc cgccgaaccc aacaggagca gccgaataaa
    12361 aagggatttc ataatgccgt cctcccgacg cggcaacggg gtgttttatc aaaacggcaa
    12421 acttgccaat gcggtttccg attgcagatt gccaaaccgg caaacctttc cccgtgccga
    12481 tgccgaacct gatgccgtct gaaccttcag acggcatcgg atgtttattt gtccacccgg
    12541 atgggtgcag gttcatattg tgtcgattcg tcgccgtaat acagcacgcc gagtttgatg
    12601 gggattctgc cctgcgattt gcggtgggca ttggaatcgc gcaaggaata ggcgcaaccg
    12661 caatattcct gctggtagaa gttttcacgt ttgctgattt caatcatgcg cgcgctgccg
    12721 ccgcctttgc gccagttgaa atcccaatac accacatcat cgtaaggcgc ggcggcgcgg
    12781 tgtccgcagt cgttgatttg cgccatattt ttccagcgtg aaatgcccag cgaactggta
    12841 aagacgggga acccgtgttc atgcgcgtat tgcgccgcct tttcaaaacg catatcgaaa
    12901 cacatggtgc agcggatgcc gcgttccggc tcaaactcca tgcctttggc tttggcaaac
    12961 cattctttgc ggtcgttttc gtagtcgtcg tctttatcga tgaaaggaat gccgaacttt
    13021 tccgcaaagc gcacgttttc ctcttttcgg agcatatact ctttgtgcgg atggatattg
    13081 ggattgtaaa aataaatggt gtagtcgatg ccggaggcaa gcatcgcctc catcacttcg
    13141 ccgctgcacg gggcgcagca ggaatgcagc aagacttttt tatgtccgcc gggcgggacg
    13201 agtatggggc ggtcaatgtc ggtaacggta ggtttgtttt gtgtttccat taacttaacg
    13261 atatctgtcg aatacggttg aagcggctat tttatcagag ccgccgcatg taaaacaggg
    13321 tgtcagggca tcagcccgag ctgcctgaac gaacggacgg tttgcgccgt tacgataaaa
    13381 tggtcgagca gcgacacatc gaccagcgac attgcctgtg ccagccgcct tgtgaacatg
    13441 atgtcttcct gcgaaggttc gggcgagccg cccggatggt tgtgcgcgat aatcaggctg
    13501 tcggcatatt cgtccaatgc cagtttgaca atttcacgga tgtaaatcgt gttttccgca
    13561 accgtaccgc gcgacagctc tctgaccgca atcagttggt tttggcggtt cagcagcagc
    13621 gcgacgctga cttcgacttt ttcctgcccc aaatgaaagc gtaaataatc ggccaccgta
    13681 tccggatcgg acaggacgat gctctcctgc aattcttccg ccaatatccg ccgcccgatt
    13741 tccctgacca cggcaaactg tgtgaaactt gccgtcccca tccctttgta tgccgacagt
    13801 ttgccgacct ccgcgctcat cagcctcccc aaactgccga actcctgcag caaataacgc
    13861 gccaaatcga ccgcactcat tccgcgcgtg ccgacgcgca gcaggattgc caaaagttcg
    13921 gcatcactca acgccgccgc cccgcgttcc aacagctttt ccctgggtct ttcgccttcc
    13981 ggccattgct tgatgctcat gttttccctt ttttaaatag tctgagatgc cgtctgaaac
    14041 agatgttccc acacgccaaa tatccgaaat gccgtctgaa tcggatttcc gcttcagacg
    14101 gcatctgtat ttcaaaacag tcttacgccg tttcttccaa ctcgattgaa gccgccagaa
    14161 gcgacaggcg tgccattacg ccgtaaacgt agaggcggtt gtgttcgttg tccacgtcgc
    14221 agtctttttc agagcgcggc atacccagcg agtaccattg ttcgaatacg cgcttgcagg
    14281 cttcgggcgc ttcttgggaa ttatcgccgt taccggtagg aatgctgtta gacagcggaa
    14341 caaacaccat accgccggcg ttgaggtttt cgtccgcacc gcgcccttcg tgtacgcgga
    14401 aaaagccgcc gatgacgaag cggtccatca tatacacgac gggttcgcac accgcgccgt
    14461 ttaaggtttc ataagtataa atcccttctt ggacaatcac ttcgctgact tccaagcctt
    14521 ctttgacttt cgccatttta ttgcggtttt tacggttcaa tccgcgcact tcgtcggcgg
    14581 atttgacgct catcacgccc atgccgtaag tgccggcatc ggctttgacg atgacgaaag
    14641 gtttgtcggt aatgcccgat tcgtcgtatt tggcttgaat tttcgccagc acacgttcta
    14701 ccgcttccgc caacgcgtct tcgccttcac gcccttggaa gtccaaaccg ccgatttttt
    14761 caaaataagg gttaatttgc cattcgtcga tgccgattaa cttggcaaat tcggcggtaa
    14821 cttggttgta cgcgccgaaa tgatttgttt tgcggcgcgt cgtccaaccg ccgtgcaacg
    14881 gcggcaaaac ggtttggctg atgcctttga ggatgtcggg aatgccggca gacaaatcgt
    14941 tgttcaacaa aaccacgcaa ggcgaaaagc cgtctgcaag atggacgcgg tcgcgggtac
    15001 gcaataaagg ttccaacagg attttgtcgc ccaatgcagt ttcaaattcg gtcggttcgg
    15061 ttacttccgg attcaggctg cccaagcgca cttcataccc tgccgaacgc aaaatctcgc
    15121 cgagggcgta aatgttttgc aggtaaaacg tattgcgcgt gtggttttcg ggaataatca
    15181 atacggattt cgccgtttcg caggcgcgtt gcaccgcatc ttgcgcggca accgccgcca
    15241 gcgggataaa gttgggattc agattattga agccgccggg gaacaaattc atatcgatag
    15301 acgaaatttt gtaaccggca ttgcgtatat cgaccgaacc gtaaaacggc gggcggtgta
    15361 cgctccactg cgtgcggaac cacgcctcga ttttggcgtg attggacagg atttttgcct
    15421 caaacgcctg aagttgcgcc gaatgttcgg gcgacatcac cggtaatttc atctttcgtc
    15481 tgcctctgtg tgttttttga caagatgcag atgataatgc ctttgaaaga atgacgacaa
    15541 gtattctttc gtatcgaatc atgctttaga tataactgtt tactgaaatg gacaatacgt
    15601 ctaatttttt cgttaaaatt gtttgcaaaa taacaccggc tcaaaaatta gacaaaattt
    15661 gttgcgcggt ataaagaata cgtctaaaat ccgcccaatc cgcattacca tttatccaaa
    15721 agaacaacat catgacagca caaaccctct acgacaaact ttggaacagc cacgtcgtcc
    15781 gcgaagaaga agacggcacc gtcctgctct acatcgaccg ccatttggtg cacgaagtta
    15841 ccagccctca ggcatttgaa ggcttgaaaa tggcggggcg caagctgtgg cgcatcgaca
    15901 gcgtcgtctc caccgccgac cacaacaccc cgaccggcga ttgggacaaa ggcatccaag
    15961 acccgatttc caagctgcaa gtcgatactt tggacaaaaa cattaaagag tttggcgcac
    16021 tcgcctattt tccgtttatg gacaaaggtc agggcatcgt acacgttatg ggccccgaac
    16081 aaggcgcgac cctgcccggt atgaccgtcg tctgcggcga ctcgcacact tccacccacg
    16141 gcgcattcgg cgcactggcg cacggcatcg gcacttccga agtcgagcac acgatggcga
    16201 cccagtgcat taccgcgaaa aaatccaaat ccatgctgat tgccgttgac ggcaaattaa
    16261 aagcgggcgt taccgccaaa gacgtggcac tttacatcat cgggcaaatc ggcacggcgg
    16321 gcggtacagg ctacgccgtc gagtttggcg gcgaagccat ccgcagcctt tctatggaag
    16381 gccgcatgac tttatgcaat atggcgatcg aagccggtgc gcgctcaggc atggttgccg
    16441 tcgaccaaac caccatcgac tatgtaaaag ataaaccctt cgcacccgaa ggcgaagcgt
    16501 gggacaaagc cgtcgagtac tggcgcacgc tggtgtctga cgaaggtgcg gtattcgaca
    16561 aagaataccg tttcaacgcc gaagacatcg aaccgcaagt cacttggggt acctcgcctg
    16621 aaatggtttt agacatcagc agcaaagtgc cgaatcctgc cgaagaaacc gatccggtca
    16681 aacgcagcgg tatggaacgc gcccttgaat acatgggctt ggaagccggt acgccattaa
    16741 acgaaatccc cgtcgacatc gtattcatcg gctcttgcac caacagccgc atcgaagact
    16801 tgcgcgaagc cgccgccatc gccaaagacc gcaaaaaagc cgccaacgta cagcgcgtgt
    16861 taatcgtccc cggctccggt ttggttaaag aacaagccga aaaagaaggc ttggacaaaa
    16921 ttttcattga agccggtttt gaatggcgcg aaccgggctg ttcaatgtgt ctcgccatga
    16981 acgccgaccg cctcgcaccg gggcaacgct gcgcctccac ctccaaccgc aattttgaag
    17041 gccgtcaagg caacggcgga cgtacccacc tcgtcagccc tgccatggca gccgcagccg
    17101 cagtgaccgg tcgctttacc gacatccgca tgatggcgta agtattttca ggcggtcttt
    17161 aagaatttga cgcggccgtc tgaaatatgt cccgtaaggc agaaacacct ttaatattca
    17221 aaccaaggag tttaaccatg aacaaacttt tcattaccgc cctgtccgcc ctcgccttgt
    17281 ccgcctgcgc cggcacttgg gagggcgcga aacaagacac cgcccgcaac cttgacaaaa
    17341 cacaggccgc cgccgaacgc gccgccgaac aaacaggcaa cgccgtcgaa aaaggctggg
    17401 acaaaaccaa agaagccgtc aaaaaaggcg gcaatgccgt cggacgcggc atttcccatc
    17461 tcggcggaaa aatcgaaaac gccaccgaat aaccgattcg cgatgccgtc tgaaaccgct
    17521 tcagacggca tcgtcagcac aagacgcaaa cccatgaaag cctttaccaa aattaccgcc
    17581 atcgtcgccc cgctcgaccg cagcaacgtc gataccgacg ccatcatccc caaacaattt
    17641 ttaaaatcca tcaaacgcag cggcttcggc cccaatgcct ttgacgaatg gcgttacctc
    17701 gaccacggcg aaccgggcat ggacaacagc aaacgcccgt tgaaccctga tttctcgctg
    17761 aaccagccgc gttaccaagg cgcgcaaatc ctgttgacgc gtaaaaactt cggttgcggc
    17821 tcttcacgcg aacacgcccc ttgggcattg gacgactacg gcttccgcgc cgtcatcgcc
    17881 cccagcttcg ccgacatctt ctttaacaac tgctacaaaa acggcctttt gcctatcgtg
    17941 ttgaccgaag aacaggttga ccggcttttc aaagaagttg aagccaacga aggctaccgg
    18001 ctctccatcg accttgccgg gcaaaccctg accacccccg gcggcgaaac attcgcattc
    18061 gacattaccg aacaccgcaa acactgcctc ttaaacggct tggacgaaat cggactgacc
    18121 ctgcaacacg ccgatgaaat tcacgccttt gaagaaaaac gccgccaaag ccagccttgg
    18181 ctgtttaacg gttaaaccta tgccgtctga aggccgttca gacggcattc ctgataacat
    18241 ataattgctg caactaatgt aaacagaggg gctataaatt gctttcatct ttcaagcggg
    18301 cttagcaacg ctctaaatta atgcaataca gctctatttc aaataaattt ttagattttt
    18361 aatatgcaac agattaaatt tattgactta ttttccggga tgagcggtat caggaaagga
    18421 tttgaacaag cctgtcggaa acagtcggtt gcttgcgaat gtgtttttac ctcagaaatc
    18481 aaaccggcag ctttggaagt attgaagcaa aactaccctg atgaagtgcc gtatggagat
    18541 ataacgaaaa ttgaaacggg agatattccc gattttgaca tcttgttggc aggcttccct
    18601 tgccaggctt tttctttcgc cggaaaaaga ttgggctttg aagatacgcg gggaacgctt
    18661 ttctttgatg tggcaaggat tttaaaggca aaaaaaccaa aaggttttat tttggaaaat
    18721 gtggaaggat tggtgacgca cgatagaaaa gatccgacac aaaaaatagg acgcaccctg
    18781 accgttattt tggaaacctt ggaaacattg ggctactatg tttcttggaa agttttaaat
    18841 gcaaaagatt tcggcattcc ccaaaacagg aagcgcattt atctgacagg cagtttgaaa
    18901 tccaaacccg acttgtcttt tgaaacaacc ctaagtccga aattaaaaaa tattttggaa
    18961 tcgggactgc ctaccgaaag cagtcctttc atcaaaaaat tgctaaagaa atttcccccg
    19021 tccgaactgt acggaaaatc agtaaaggac aaacggggag ggaaaaacaa tatccacagt
    19081 tgggatattg aattaaaagg cacagtaacc gaagaagaga agcaattatt aaatatgctt
    19141 ctaaaagaac ggaggaagaa aaaatgggct tcagaaatcg gcatcgattg gatggatggg
    19201 atgcctttga caaaagcgca aatttcaact ttctataaac accccaatct tcaaaatatt
    19261 ttggacagcc tgacagataa aggctatttg gttttggagc atccgaaaca aaaaattggc
    19321 gggcgaagaa tcaaagatga atccctgccc aaaggctaca atattgtttc aggaaaaaaa
    19381 tcatttgaaa tcaataaaat attagaccca aatgatgttg cgccaacctt agttgcaatg
    19441 gatatggagc accttttcgt cgttgacaac ggcggtttgc gcacacttac cggaaaagaa
    19501 ggtttacgct tattcggcta tccggacgat tattcgtttg atattcctaa aaaagacaaa
    19561 tacgatttat tgggcaatac cgttgccgtt cctgtgatta aggcggtatc tgaaaggctt
    19621 ctgcatactt tataaaccga tagggataaa atggtaaaac ttactgcaca acaaatattt
    19681 gataagttgc tgaatgagga aaaaatctta tcggtcaatg gtcaaattag atttttcttg
    19741 ggcgatgtgg atattatcgt caaacaaaaa gatgtcgtcg gcaacatcat tcaagaatgg
    19801 ctcggcggat ggttgagaaa aagggaaatt gaatttgatg tttcaaccaa tacccaaatg
    19861 ccccccgatt tctttttaaa taaaaaagac cgcagtagag aattgttgga agtaaaggcg
    19921 ttcaaccgaa atgccggccc gggttttgat attgcagatt ttaaaatgta ttctgatgaa
    19981 atcattcata aaccctatat gctggatgta gattatttaa tattcggtta cgatatggac
    20041 gacaacggca atgtaaccat caaggattta tggcttaaaa aagtatggca aattacgaga
    20101 agcatggatg ggtgggcaat taatcttcaa gtcaaaaaag gcgtggtgca taaaatccgc
    20161 ccgggtgttt ggtacagcat aaataaaaag aatatgccta tgtttgaatg cttggaagat
    20221 tttgtttccg caattgaaga aaccgtttat caaaacccgg caacacggca taacgcatcc
    20281 ttatggaaaa agaaatttga ggaagcctat aaaaaacact acaaccgatc gatttccata
    20341 cctcgttggc acgaaattgc acacaaatac aaaaagaaat aacccttcaa ccatcaaccc
    20401 ataaggaaac ttgaaatgac caaacatatc gccatcctcc gaggtgacgg catcggtccc
    20461 gaaatcgtcg ccgaaaccgt ccgcgtactc gacaaactca tcgcccaagg cttggacatc
    20521 agctacgaat acgccccatt gggtggtgaa gcctacgacg aatacggcca tccttatccc
    20581 gaatttacgc aaaacctctg ccgcaaagcc gatgccgtcc tgcttggtgc agtcggttct
    20641 cctcaatacg acaacctcga ccgtccgttg cgccctgagc gcggcctgtt ggcaatccgt
    20701 aaggatttga acctgtttgc caatttgcgt cctgctgttt tatatcccga attagccaac
    20761 gcctccacgc tgaagcctga aatcgttgcc ggtttggata ttttgattgt gcgcgaactc
    20821 accggcgaca tctatttcgg cgaaccgcgc ggcatccgcg ttctggaaaa cggcgaacgc
    20881 gaaggctaca acaccatgaa atacagcgaa agcgaaatcc gccgtattgc ccatgttgcc
    20941 ttccaatccg cccaaaaacg cagcaaaaaa gtctgttccg taggcaaagc caacgttttg
    21001 gaaaccaccg aactgtggcg cgaaatcttt gaagaaatcg gcaaacaata tcccgatgtc
    21061 gaactctccc atatgtatgt ggacaacgcc gctatgcagc tcgtgcgcgc gcccaaacaa
    21121 tttgacgtga ttgccaccgg caacatcttc ggcgatattt tatcggacga ggcctctatg
    21181 ctgaccggct ccatcggtat gctgccttct gcttctttgg acgaaaacgg caaaggcctg
    21241 tacgaaccgt ctcacggctc cgctcccgac atcgccggac aaaacaaagc caacccgctg
    21301 gccaccatcc tctcgctcgc aatgttgctg cgttacagcc tgaacgacga agcgcgcgcg
    21361 caacaagttg aaaacgccgt ccaaaaagtg ctgcaacaag gcttgcgtac cagcgacatt
    21421 tacgaagaag gcacaaaact cgtttcctgc tccgaaatgg gcgatgcgat actcgccgcc
    21481 ttgtaaaaaa acaaagcgcg gatcacaccg cgctttttca tctgcccttt tccgcccgca
    21541 ccgttcgtca accgcctgca aacccaccgt ttaccggcag gaaatgccat aaatccccgc
    21601 ctatgttagg ataaacacct ttatcggccc acacaaagga ttatcatcat gaaaaaaatc
    21661 atcttcgccg cactcgcagc ggcagccgtt ggcactgcct ccgccgccac ctacaaagtg
    21721 gacgaatatc acgccaacgc ccgtttcgcc atcgaccatt tcaacaccag caccaacgtc
    21781 ggcggttttt acggtctgac cggttccgtc gagttcgacc aagcaaaacg cgacggtaaa
    21841 atcgacatca ccatccccgt tgccaacctg caaagcggtt cgcaacactt taccgaccac
    21901 ctgaaatcag ccgacatctt cgatgccgcc caatatccgg acatccgctt tgtttccacc
    21961 aaattcaact tcaacggcaa aaaactggtt tccgttgacg gcaacctgac catgcacggc
    22021 aaaaccgccc ccgtcaaact caaagccgaa aaattcaact gctaccaaag cccgatggcg
    22081 aaaaccgaag tttgcggcgg cgacttcagc accaccatcg accgcaccaa atggggcgtg
    22141 gactacctcg ttaacgttgg tatgaccaaa agcgtccgca tcgacatcca aatcgaggca
    22201 gccaaacaat aaatcttgat gcgaatcagc acaaatgccg tcttcatcaa accgccccgc
    22261 caacggtaag ccgatatggc ggagcacaat ccgccgaaag atatttcagc ggcaggcagg
    22321 gcttgccgga aagtccggaa agtatagtgg attaacaaaa accagtacgg cgtt
//
LOCUS       DAXHTR010000012        22126 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.012, whole genome shotgun sequence.
ACCESSION   DAXHTR010000012 DAXHTR010000000
VERSION     DAXHTR010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22126
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.012"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            153..1058
                     /locus_tag="ACFNML_000343"
     CDS             153..1058
                     /locus_tag="ACFNML_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6391792.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGPYAI
                     AFWRLLISVFVFWFLARFFGQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFNERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFA
                     SGLVSGLMLALSMVFVRKTHEIEPVALFPSMMILSLGGAVSLVVPALLMDGGVLYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
     gene            1104..1253
                     /locus_tag="ACFNML_000344"
     CDS             1104..1253
                     /locus_tag="ACFNML_000344"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391793.1"
                     /translation="MNWQAVKTGKTKHASNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="ACFNML_000345"
     CDS             complement(1472..2491)
                     /gene="lepB"
                     /locus_tag="ACFNML_000345"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFC6391794.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFAAVFLIFVVLCGTAWAVYKYRLKTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNIFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRDKILTVNGKPTSDIPNGTYRYPDDTDP
                     SEIHNTDMFRSDLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRS
                     GTAIR"
     gene            complement(2636..4429)
                     /gene="lepA"
                     /locus_tag="ACFNML_000346"
     CDS             complement(2636..4429)
                     /gene="lepA"
                     /locus_tag="ACFNML_000346"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFC6391795.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPATEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKNGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            complement(4571..5272)
                     /locus_tag="ACFNML_000347"
     CDS             complement(4571..5272)
                     /locus_tag="ACFNML_000347"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFC6391796.1"
                     /translation="MSLKTVAVIGAMEQEIKLLREMMENIKAVSFGRFSAYEGELVGK
                     RMVLALSGIGKVNAAVATAWLIHQFAPDCVINTGSAGGLGKGLKVGDVVIGTETAHHD
                     VDVTAFGYVWGQVPQLPARFASDGILIEAAKRAARTFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPEVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AANSAKMVAEIVKSL"
     gene            5440..6570
                     /locus_tag="ACFNML_000348"
     CDS             5440..6570
                     /locus_tag="ACFNML_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6391797.1"
                     /translation="MFTDENMTAKEELFAWLRHMNKNKGSDLFVTTHFPPAMKLDGKI
                     TRITDEPLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGAT
                     ALVFRAITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNEN
                     SFGHIITIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDR
                     ETMDYAIAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQ
                     RLVPRDGGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQL
                     YEKGDISLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            6604..7581
                     /locus_tag="ACFNML_000349"
     CDS             6604..7581
                     /locus_tag="ACFNML_000349"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFC6391798.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPRVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLDVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TASGFRLRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            7585..7935
                     /locus_tag="ACFNML_000350"
     CDS             7585..7935
                     /locus_tag="ACFNML_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HFC6391799.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            7940..8719
                     /locus_tag="ACFNML_000351"
     CDS             7940..8719
                     /locus_tag="ACFNML_000351"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HFC6391800.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     AEVSAIAEVYEHIYCTIGVHPDSKEAEEFSIAEMVAAAASPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRHTAEHIAKLRNQTLEQVAAYTTENFYRLFKKVPDMRTV"
     gene            8745..8959
                     /locus_tag="ACFNML_000352"
                     /pseudo
     CDS             8745..8959
                     /locus_tag="ACFNML_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
     gene            9060..9371
                     /gene="grxD"
                     /locus_tag="ACFNML_000353"
     CDS             9060..9371
                     /gene="grxD"
                     /locus_tag="ACFNML_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HFC6391801.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            9477..10103
                     /gene="upp"
                     /locus_tag="ACFNML_000354"
     CDS             9477..10103
                     /gene="upp"
                     /locus_tag="ACFNML_000354"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006223 - uracil salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFC6391802.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            10126..10446
                     /locus_tag="ACFNML_000355"
     CDS             10126..10446
                     /locus_tag="ACFNML_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HFC6391803.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            10697..11116
                     /locus_tag="ACFNML_000356"
     CDS             10697..11116
                     /locus_tag="ACFNML_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein Gly1ORF1"
                     /protein_id="HFC6391804.1"
                     /translation="MKKMFLSAVLLLSAATRTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            11137..11898
                     /locus_tag="ACFNML_000357"
     CDS             11137..11898
                     /locus_tag="ACFNML_000357"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFC6391805.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKAQIAVGQGSRRALERCLVRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGFRTEVAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            11973..13247
                     /locus_tag="ACFNML_000358"
     CDS             11973..13247
                     /locus_tag="ACFNML_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFC6391806.1"
                     /translation="MPSETSSPRKENETEVHIPAAPFIVKQSGNNALATCALVLAALG
                     LGASGFLFVQGQNVLKNQELAFNQKIDKAALGESENAALLKDNLNRQAAIQSELDRLD
                     GNVKANGEQILEMQKSYRELTKGRADWLVDETETILNLAAQQLVLTGNIQTAVGVLEH
                     IDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGTALRLDRLETAVSGLPLILDG
                     VLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRLENNDAMLISPEQAYFVRENL
                     RLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKSPATQSWLKELAELKALDVRM
                     TADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPANENTASEPAAASDVKTIEAP
                     SLPSERKPEQPAKKQPVPEKAGRSPSAKGERA"
     gene            13244..14461
                     /locus_tag="ACFNML_000359"
     CDS             13244..14461
                     /locus_tag="ACFNML_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HFC6391807.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLVNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEAFVESVRFLGEREQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDETGEPQKAEAQRNLVLA
                     SVAEENRPSAETH"
     gene            14536..15600
                     /gene="hemE"
                     /locus_tag="ACFNML_000360"
     CDS             14536..15600
                     /gene="hemE"
                     /locus_tag="ACFNML_000360"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFC6391808.1"
                     /translation="MTLLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTGLATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVSSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAEIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            15778..17157
                     /gene="radA"
                     /locus_tag="ACFNML_000361"
     CDS             15778..17157
                     /gene="radA"
                     /locus_tag="ACFNML_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFC6391809.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTEGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPLPEKMVAF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(17433..17912)
                     /locus_tag="ACFNML_000362"
     CDS             complement(17433..17912)
                     /locus_tag="ACFNML_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HFC6391810.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPQTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTKTDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(18037..18396)
                     /locus_tag="ACFNML_000363"
     CDS             complement(18037..18396)
                     /locus_tag="ACFNML_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6391811.1"
                     /translation="MNIKHLITTALIASAAFAAQAAPQKPLSAAQTAQHPVVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            complement(18435..22049)
                     /gene="recB"
                     /locus_tag="ACFNML_000364"
     CDS             complement(18435..22049)
                     /gene="recB"
                     /locus_tag="ACFNML_000364"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFC6391812.1"
                     /translation="MSAPIQAFDSLTVPIAGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKNVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAELGDGALSDGIAAYCAE
                     HHKGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLADFGRD
                     LNALEEAEEATMIRLQLDLIEYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPKRPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAAECRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPHRA
                     GTLRFVLTSGIFGYDAQQLHDFNQNESEILHWAESARTALDNWNQYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLRDQISLAGNNGGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQSANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTEDAPPPAAYRGNAGQTAE
                     FAANSIPERRFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAEISVAAMPSETPTAS
                     DGISIHNFPKGTQAGLCLHEILEDFKFGQAAAGQETLITDKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYGLSDIPPECRCPEMGFTLHTEDFGLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFIDMVCQDPEGNICVIDYKSNHLGADASAYTRQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
BASE COUNT         4996 a         5741 c         6276 g         5113 t
ORIGIN      
        1 gggttttatc tggcaggacg gttttttgcc ccaacggaaa atagtcggcc tgcccgtaaa
       61 atcagccgtt tgtccgggtg cagccggggc tttgggcttc agacggcata ttttcggaat
      121 ggcggcattc ttgccgtcgg cgcggcagcc gtatggggaa gggaggggat attgtggtcg
      181 gtaacggcaa aaaatatgcc gcaccattgc tggtgctggg ttgcgtggtg ttcggtctgg
      241 gcagtctgat tgtcaggtcc gtccccgtcg gcccgtatgc aatcgcattt tggcggttgc
      301 tgatttcggt gttcgtattt tggtttttgg cacggttttt cgggcaaaaa ttcccaaaaa
      361 acaggaaaac cgtccgatat gccctgacgg cgggcgtgtt cctcgctttc gatttggcgt
      421 tgtggcacga aagcatacac gcggtcgggc cgggtatttc caccctgctc aacagcctgc
      481 aaatcttttt cttgtcggca atcggtgttt tcttttttaa cgaacgtttg agcgggctga
      541 aaaaggcagg cttaatatcg gcagttgccg gcgtggcgat gattgccggt gcggaattcg
      601 gctacaacgg taatgcggtt tggggattcg ccagcggttt ggtatcggga ctgatgctcg
      661 ccctgtcgat ggtgttcgtc cgcaaaaccc atgaaatcga gccggtggcg cttttccctt
      721 caatgatgat tttgagtttg ggcggcgcgg tatcgctggt tgttccggca ttgctgatgg
      781 atggcggcgt gctttatccg acgacttgga aagatgcggg tttggtgctt gtgtacggcg
      841 tggtgatgca gtgcttcgcg tgggcgatgg ttgcctatgc gattccgctg ctttcgctgt
      901 cgctgacggg gctgctgctt ttgtccgaac cggttgccgc cctgttcatc gattatttcg
      961 ggttgggcaa accgattgaa ggcgtgcagt gggcaggggt ggcgctgacg ctttcggcaa
     1021 tttacctcgg ttcgctgaaa cagcagtccg agccttgact ccgtccggca tcagattttc
     1081 tgccgtggca aacctgcgta cgattgaact ggcaggcagt caaaaccggc aagaccaaac
     1141 acgccagcaa cggcgcggag cggaaaatgc cgtctgaagc cctttcagac ggcatcgggg
     1201 cgggcagaat caaaaaagcc gcctccgctg gaatggcaat gcaactaaaa taatgtccgt
     1261 atttccatat gccgcccaca ccgtcagtcg gacgaaatgc cctttattca atgggatggg
     1321 tttccacaca aacggaaata tagtggatta aatttaatcc actatattat ccccgaggct
     1381 gccggcaggg aaacaaggcg tttgatgata aaaatgccgt ctgaaacagg gttcggatgg
     1441 catttttgat gttccggcgt aattttccgg cctaacggat tgccgtaccg gacctgccga
     1501 aatcgccgaa gttcatcaaa atgaacattg ccttgccgac aaccagcttg tcatccacaa
     1561 atccccagta gcgcgaatcg gcactgttgt cgcggttgtc gcccatagcg aaatagcgtc
     1621 cttcgggaac tttgcacacg aaaccgctgc cgtcgtcggc atattggcag tgttccaaac
     1681 cgctttgctc tatggaatat ccgttttcag acataatatc ggaggtatat ttgcccaata
     1741 cgggcaggga aacggcaggc tgtccttctt ttttcagaat attgaaggat ttgccgtcca
     1801 gatcgctgcg gaacatatcc gtgttgtgga tttcggaagg gtcggtgtcg tcgggataac
     1861 ggtatgtgcc gttaggaata tcggaagtgg gtttgccatt taccgtcaaa atcttatccc
     1921 gatattcgac cacatcgccc ggaatgccga caatacgctt gatgtaggtc atctccggct
     1981 gcagaggata attaaaaaca acgacatcgc ctcgttcgat tttgcctgta ggaataaata
     2041 tattgtttaa aacgggtacg cgcagaccgt aggaaaattt gccgaccaaa atgaaatcgc
     2101 ccttaatcag gcccgggcgc atcgagctgg acgggatttg gaacggttcg gcaataaacg
     2161 accggatgag gaacaatacc aaaacggtag ggaagaaact gccgaaataa tcgccgaagt
     2221 ggctgctttc cgagatttcg ggatgagtct tcaggcggta tttgtatacc gcccaagccg
     2281 taccgcacaa tacaacgaaa atcaggaaaa cggcggcaaa gctcataaac agggacaaag
     2341 cggcaaacac gccgaccgcc gtcaggatat aggcgtattc gaggccggaa ctccattcac
     2401 cgttttcctg ccgcttcttg tcgcttttga aataaaggat gatgccggca agcagcgcgg
     2461 cagccgcgcc cgacattagc attgtgttca ttgttgttcc tcaacggtta aaaacccgcc
     2521 cggccgtgca accgttttaa ggcggcaaat tgcaaaattt gtttgcgggc gcgtgcccct
     2581 gaaatcaggg cggtttgaga ggtgttcccg acgcgccgcc ctatgtgccg aaatattatt
     2641 tgtcgctcac ctgcaaaatc gccaagaacg cgctttgcgg gatttccaca ttgcccactt
     2701 gtttcatacg gcgtttacct gccttttgtt tttcaagcag ttttttctta cgcgtaatat
     2761 cgccgccgta acatttcgcc aagacgtttt tacgcagtgc tttgacgttt tcgcgggcga
     2821 taatctggct gccgatggcg gcttggacgg caatgtcgaa catttggcgc ggaatcagct
     2881 cgcgcatttt cgatgccagc tcgcggcctc ggtgaaccgc gctttgacgg tgcacaatca
     2941 ggcttaaggc atcgactttt tcgccgttga ccatgatatc cagcttaatc aaatcagacg
     3001 gttggaactc tttgaaatgg tagtccaacg aagcataacc gcgcgaagtg gatttgagtt
     3061 tgtcgaagaa gtccatgacg acttcgttca tgggcaaatc gtaagtcagc atgacttggc
     3121 ggcccatata ttgcatattg acctgaacgc cgcgcttttg gttacacaaa gtcatgacgt
     3181 tgccgacgta ttcctgcggc acgaggatgg tcgcggtaat aatcggctcg agtatggttt
     3241 cgatgctgcc gatgtcgggc agtttggacg ggttttcaac ttcgattttc tcaccgtttt
     3301 tcaacacgac ttcgtaaacc accgtcggcg cggtggtaat caagtccatg tcgaactcgc
     3361 gctccaaacg ttcctgcacg atttccaagt gcagcagacc caagaagccg caacggaagc
     3421 cgaagcccaa tgcctgggaa acttcaggct cgaatttcaa cgaagcgtcg ttaagctgca
     3481 atttttccaa agcgtcgcgc aaggcttcgt agtcgtggct ttccacggga taaagtccgg
     3541 cgaatacttg gctttgtact tcttggaagc cgggcagcgg ctcagtggca gggttggcaa
     3601 ccagtgtaac cgtatcgcca actttcgcct gacccaattc ttttacgcca gtaatcaaaa
     3661 agcccacttc gccggctttg agttcttgtt tttgaactga ttttggtgtg aatacgccca
     3721 gctgctcgac ctgcgtttcc gccttggtgc tcataaagcg cactttgtct ttcagtttga
     3781 tggtgccgtt tttcactcga atcagcatga ccacgccgac atagttgtca aaccacgaat
     3841 cgacgatcac ggcttgcagc ggcgcgtttt cgtcgccggt cggcgcaggg attttggcaa
     3901 cgatttcttc taaaacgtct tccacgccga tgccgctttt ggcggaacat tgcaccgcac
     3961 cgacggcatc gatgccgata atgtcttcga tttcttgttc cacgcgttcg gggtcggcgg
     4021 cgggcaggtc gattttgttt aaaacaggca caacctccac gcccaaatca atcgcggtat
     4081 agcagttcgc cacagtttgc gcttccacgc cctgcgacgc gtcaacgacc aaaagcgcgc
     4141 cttcgcaggc agagagcgaa cgagagactt cgtaagagaa gtcgacgtgt cccggcgtgt
     4201 caatcaggtt gagttgatac acctgcccgt cgcgtgcttt atagttgagc gcggcggttt
     4261 gcgctttaat ggtaatgccg cgctcttttt cgatgtccat ggaatcgagt acctgcgtac
     4321 tcatttcgcg caaatccaaa ccgccgcaat attggatgaa gcggtcggca agcgtcgatt
     4381 tgccgtggtc gatgtgggca atgatggaga aatttcggat atttttcatt gagttgtttt
     4441 taatgctgaa cggtaggttt tagaaatgcc gtctgaacaa acagcggtac gtccaaatat
     4501 cgggcgcaac gtggaaatag cccgatattc taacggaaaa ccgctgtttt ggcatgattt
     4561 gataaaggtt ttataaagat ttgacgattt ctgccaccat ttttgcggaa tttgccgccg
     4621 ccgttttcaa gaactcgtca aagctgatgt ctgccttttc gtctgccgaa tcggaaaccg
     4681 cgcggatgat gacgaaaggc gtttccaact gatgacaggt ttgggcgatt gccgccgctt
     4741 ccatttctac cgccttaact tcggggaagt gcttgcggat ttccgccacg ccttcgctgc
     4801 tgtggacgaa gcggtcgccg ctgacaatca ggccttgttc taccgccgcg ccttcaaacg
     4861 tccgcgccgc ccgttttgcc gcctcaatca aaatgccgtc tgaagcaaac cttgccggca
     4921 gttgcggcac ttgtccccaa acatagccga atgcggttac gtcgacatcg tggtgtgcgg
     4981 tttccgtacc gatgactacg tcgccgactt tcaaaccctt gcccaaaccg cccgcgctgc
     5041 cggtgttgat gacgcagtcc ggtgcgaatt gatggataag ccaagccgtt gcaaccgccg
     5101 cgttgacctt gccgatgccg ctcaatgcaa gcaccatgcg ttttcccacc aattcgcctt
     5161 cataggcgga aaatctgccg aaagagacgg ctttgatatt ttccatcatc tcgcgcaaaa
     5221 gcttgatttc ttgttccatt gcgccgataa cggctactgt tttcaaagac atattgctga
     5281 cctgttgtga atttcggata gaatgccgga ttatacacgc taacacggca ggattgagtg
     5341 ggggcggttt gtccgtgccg tctgaaacgg tttcagacgg cacggcgggt ttttggtaga
     5401 atgggaaggt acagattgtt tgaagattag gggacgagga tgtttaccga tgaaaatatg
     5461 accgcaaagg aagaactgtt cgcatggctg cgccatatga acaaaaacaa aggttccgac
     5521 ctgtttgtga cgacccattt cccgcccgca atgaagctgg acggcaaaat cacccgcatc
     5581 acggacgaac cgctgacggc ggaaaaatgt atggaaatcg ccttttcgat tatgagtgcg
     5641 aagcaggcgg aagaattttc atcgaccaac gagtgcaact tcgccatcag cctgccggac
     5701 accagccgct tccgcgtcaa tgcgatgata cagcgcggcg cgacggcgtt ggtattccgt
     5761 gcgattacca gcaagattcc caagtttgaa agcctgaacc tgccgccggt cttgaaggat
     5821 gtcgcgctga aaaaacgcgg gctggttatt tttgtcggcg gcaccggctc gggcaaatcg
     5881 acttcgcttg cctcgcttat cgattaccgc aatgaaaatt cgttcggaca catcatcacc
     5941 atcgaagacc cgatcgagtt tgtccacgaa cacaaaaact gcatcatcac ccagcgcgag
     6001 gtcggcgtgg atacggaaaa ctggatggcg gcgttgaaaa acacgctgcg tcaggcgccg
     6061 gatgtcatcc ttatcggcga aatccgcgac cgcgaaacaa tggactacgc cattgccttt
     6121 gccgaaacgg ggcatttgtg tatggcgacg ctgcacgcca acagcaccaa tcaggcactc
     6181 gaccgcatca tcaacttttt ccccgaggag cggcgcgaac aattgctgac ggatttgtcg
     6241 ctcaaccttc aggcgtttat ttcgcaacgc ctcgttccgc gagacggcgg caagggcagg
     6301 gtggcggcag tcgaggtgct gctcaattcg cccctgattt cggagttgat tcacaacggc
     6361 aacatccatg aaatcaaaga agtgatgaaa aaatccacta ccctgggtat gcagaccttc
     6421 gatcaacacc tttaccaatt gtatgaaaaa ggcgatattt ccctgcaaga agcattgaaa
     6481 aatgccgatt ccgcacacga tttgcgtttg gcggtacagt tgcgcagccg ccgcgcgcaa
     6541 agttccagcc ccgatttgga actgctctga tggcggtatg gatttccgga cggatggttt
     6601 gaaatgattt atccgtggca taatgagcaa tggcggcaga ttgcggaaca ttgggagcgt
     6661 cgtcccaatg catggctgtt tgccggcaaa aaagatacgg ggaaaactac atttgcccgc
     6721 tttgcggcga aggcactgtt gtgcgaaacc cctgcaccgg gctgcaaacc ctgtggcgaa
     6781 tgtatgtcct gccatctgtt tggacaggga agccatcccg atttttacga aatcaccccc
     6841 ttgtcggacg aacccgaaaa cggacgcaaa ctgttgcaga tcaaaatcga tgccgtcagg
     6901 gaaatcatcg ataatgtgta cctgacttcg gtacggggcg gtttgcgcgt gattctgatt
     6961 catcctgcgg aaagtatgaa tgtccaagcc gccaacagtt tgttgaaagt gttggaagag
     7021 ccgccgccac gagtggtctt tttgctggtc agccacgcgg cggacaaggt tttaccgacc
     7081 attaaaagcc gctgccggaa gatggttttg cccgcgcctt cccatgaaga ggcattggca
     7141 tatctgcgtg aaaggggtgt ggcggaacct gaggaacgtc tggctttcca ttccggagcg
     7201 ccgctgtttg atgaggcgga cggtgtccgt gcgttgcgga ttaaactgtt ggatattttg
     7261 gcagaaccaa ggttgttgaa gattttggat tacgccgcgc ttttcgataa ggagaaactt
     7321 ccgctcgacg tatttgtcgg gtggatgcag aaatggctgg tcgatttggg attgtgcctg
     7381 caacacatga aacccgtcta ttatcccgct tatgaagaca ggctgcttca gacggcatcc
     7441 ggtttccgcc tgcgcaatgt atttgcggcg gaggatatgc tcaaacagct tgccccctac
     7501 gggtttcata ctttaaatgt caaaatgcag atcgagcatc tgctcatcaa ctatttggaa
     7561 ttgaagaaag agaacgggtg aattatgtca gacggacaaa atattccggc aaaaatgatg
     7621 tcgttgcagc tgaaagacat gaatctgctg tacagctcct acatgccgtt tttggaacac
     7681 ggcggtctgt ttgtgcagac caacgacgta ttttccatcg gggacgatat tctgcttgcc
     7741 gtagaaatcc tcaacttccc caaactgttc ctgccgacca aagtcgcctg gatcaatcct
     7801 gcgcgtactt cctccaaacc caaaggggtg gggctggcat tcacaaaaca cgaaaactgc
     7861 ctgaaagtca aagaccagat cgaagtcgaa ctgggcaaca caatcggcgg cagcagacct
     7921 acgtttacca tgtaacgcca tgcatatcat cgattcgcac tgccacctca attttgaagg
     7981 tttgaaagaa cgcctgcccg aagttttgtc caacatggaa gcaaacggcg tggggcaggc
     8041 gctcgccatc agcgtcagca gggaaagttt tgccgaagta tccgctatcg ccgaagttta
     8101 tgaacatatt tactgcacaa taggcgtaca tcccgacagc aaggaagccg aagaattttc
     8161 tattgcggaa atggttgccg cagccgcctc gccgaaagtg gtcggcattg gcgagacggg
     8221 tttggattat tactggtgca aaggcgattt gtcctggcaa cacaaacgct ttgcagacca
     8281 catcgaagca gccaatcaaa ccggactgcc cgttatcgtc catacgcgtg atgcggcggc
     8341 ggacaccttg tctatcctga aagaatgccg ggttaattcg ggcgttatcc actgtttttc
     8401 cgaagacatc ggttttgccc gtgcagcaat ggatttgggg ctttatattt ctttctcggg
     8461 aatcgttacc tttaaaaacg cacccttggt tcaggaggcg gcaaaatatg tgccggacga
     8521 ccgcattttg gtggaaaccg atgcgccgtt cctcgccccc gttcccaaac gaggcaggca
     8581 gaacgaaccg gcttttgtgc gccataccgc cgaacatatc gccaaattgc ggaaccaaac
     8641 attggaacag gttgcggcat acacgacgga aaacttttac cggctgttta aaaaagtacc
     8701 cgatatgcgg accgtctgac cctgtgccga cgataaggaa aaccatgaag gcaattcatc
     8761 cgtatgtatg tccgcgctgc tgccggctgc ctgccaacac gtttcggaca ggcatggcaa
     8821 attccgcttc caaattctgc attgccaaag gcggcagacg ggaagtaaaa aagacgaaag
     8881 cggcggcgga tatgccctgt gccatttgcc ggacagcagg attgtcgagg agtgggaata
     8941 tttccgttca caacattgat actgcgcgat atacggcaaa tattgtggga agtttccgct
     9001 tttgcgtata atgcgtccta cctgacaaat tttgtcaact ttatcaaaag gaataagcga
     9061 tggcttccat ccacgaccaa attaaagaag tagtaacgac acaccgcgtc gtattgttta
     9121 tgaaaggtac gaagcagttt ccgcaatgcg gtttctcttc ccgcgccgtg caaatcctga
     9181 acgcggcggg ctgcaccgat tacgttaccg tcaacgtatt ggaaaatccc gaagtacgcc
     9241 aaggcattaa ggaatacagc gactggccga ccatccccca actttatgtg aacggcgagt
     9301 ttgtcggcgg ttcggacatc ctgatggaaa tgtatgaggc aggcgagctg caagagctgc
     9361 tgaaagcctg atggattcgg caatgccgtc tgaacgtgtt tcagacggca ttttcttttc
     9421 cggcaaatca aaaaaaagta taatggcgcg tctcaaaatc acactggaac accgcgatga
     9481 acgttaatgt tatcaaccat ccgctcgtcc gccacaaatt aaccctgatg agggaggcgg
     9541 attgcagcac ctacaaattc cggacgcttg ccatcgagct ggcgcgcctg atggcatacg
     9601 aggcaagccg tgattttgaa atcgaaaaat accttatcga cggatggtgc ggtcagattg
     9661 aaggcgatcg catcaagggc aaaacattga ccgtcgttcc catcctgcgt gcaggtttgg
     9721 gtatgcttga cggcgtactc gacctgattc cgactgccaa aatcagcgta gtcggactgc
     9781 agcgcgacga ggaaacgctg aaacccgttt cctacttcga gaaattcgtg gacagcatgg
     9841 acgaacgtcc tgccctgatt atcgacccga tgctggcgac gggcggttcg atggttgcca
     9901 ccatcgacct tttgaaagcc aagggctgca aaaacatcaa ggcactggtg ctggttgccg
     9961 cgcccgaggg tgtgaaggcg gtcaacgacg cgcaccccga cgttacgatt tacaccgccg
    10021 cgctcgacag ccacttgaac gagaacggct acatcatccc cggcttgggc gatgcgggcg
    10081 acaagatttt cggcacgcgc taactgactg attttcggat ttgatatgaa tttccaagac
    10141 tatctcgcca cattttcttc aatcgaccat ctgggcggtt tggacgttca ggatgccgac
    10201 ggcaaaacgg ttcaccacat tcccgccgtt cagggtaagc tcggttcgct caagctgtac
    10261 aatgctttgg cagaacgttt tgacggaaaa ttggacaaag aagcggcaga acagggtttg
    10321 atatggtttg ccgaacacgt tgccgacgcg cgcgcccatc ccggcaagca tcccaatatc
    10381 gacctgcttg aaaaagttgt gcaaagcggc gaaaccttgc tgctcaagcc gcttgccgcg
    10441 caataatttt cgaccatgcc gtctgaaatc cgtttcagac ggcattttgt cggaaagaag
    10501 accgtaaaac gggcattttc ttttctattt caggatacgg gcaatgatgt ttcaacacac
    10561 aggacgacac ataaagtgtt gccctatgtg ttgccctaat ttggaagggg ttacacccct
    10621 ctcaaataac atctgatgct gctgccacga aggacggatg tccaaatggc ggagtttcaa
    10681 ccattaagga aatacgatga aaaaaatgtt cctttctgcc gtattgcttc tgtcggctgc
    10741 gacccgaacc gtgtgggcgg atacggtgtt ttcctgtaaa acggacaaca acaaatatat
    10801 agaagttcaa aaaatcaacc gcaatcttta cgaatattcg ttcggtagtg cggcaaaaaa
    10861 agaaattgcc atacgcaaca gcaaagctga cctgttgggg cgttccgaca ggtggcaagg
    10921 tatgggcagc ggtcgttggg caacgatgaa attccaaaac ggcgaattta tgtacaccat
    10981 atggacaggc ttcgattccg tgactcatac ggaaagcagc ggtgtcgttg tggagcgtag
    11041 gggcaaggaa gtcgcacggg tcggctgtac gccgaaaacc gcgcaggcga atttcaacga
    11101 tgacgatttt tcctagtaat cggggcggat aaggcgatgg aaacagcgaa acccgtcatg
    11161 ctgattgtcc gtccgtcggg cagggccgca gaagatgtcg aaacttgcct gaatgccggt
    11221 tggcgcgcgg aagtattgag tccggtcgaa atcgaagcag atgctgccgg actggaactt
    11281 ttgtccgaac aatatgcccg tgcggatgcc gtgttttggg tcagtccgac cgccgttgaa
    11341 accgccgtcc cgtaccttaa cctttcagac ggcataaagg cgcagattgc cgtagggcag
    11401 ggcagccgcc gcgcattgga acgctgtttg gtcagaacgg tcatcgcgcc tgatgacggc
    11461 aacgacagcg aggcggtttt gcgcctgccg gtttggaaca gtctgcccga aggtgcgcgc
    11521 gtattgtttg tgcgcggaca cggcgggcgg gattttttga tgaatgcctt gcaggagaaa
    11581 ggttttcgga cggaggtggc agaagtctat ttcagacggc ataaaccttt gaactttcaa
    11641 aatttccaaa ccgaaaatat tgccgccgcc tatattacgt cgaccgagct ggtgcgcttg
    11701 ctgttcgggc agcttccgcc gcaattttcc cgattcttca aatccttgct atactttacc
    11761 catcatccgc gcattgcgga ggcattgaag cgcgaaggcg tgtgttcggt cgaaaccgtc
    11821 cctacgctgg aagccgcgct ttcccattct tccatttccg tttcagacgg catggtcttt
    11881 cccggaacct caaattaata aggagcaaaa cggtgggcga acctgaaaac aaatcatccg
    11941 aacccgtacg cgagatacag gcatcaaaag aaatgccgtc tgaaacctct tccccacgca
    12001 aagaaaacga aacagaagta cacattcctg ccgctccttt tatcgtcaaa cagtccggca
    12061 acaacgcttt ggcaacctgc gccttggtat tggcggcatt gggtttgggc gcaagtggtt
    12121 ttttgtttgt ccaaggacag aatgtcttga aaaaccaaga gctggcattc aaccaaaaaa
    12181 tcgacaaagc cgccttgggc gagtcggaaa acgccgccct gctcaaagac aacctcaacc
    12241 ggcaagccgc catacaatca gaactcgacc gtttggacgg aaacgtcaaa gcaaacggcg
    12301 aacaaatctt ggaaatgcaa aaatcctatc gcgagttgac caaaggacgc gccgattggc
    12361 tggtggacga aaccgagacc atactcaatc tggcggcgca acagctggtg ttgaccggca
    12421 atatccaaac ggcagtcggc gtattggagc atatcgacag ccgcctgtcc cgtttcaatc
    12481 aggcagagct tctgccgatc aaacaggcgg tcagcagcga cttggcggaa ctgaaaaacc
    12541 gtccctatgt cgatatttcc ggcacggcat tgcgcctcga caggctggaa accgccgtat
    12601 ccggactgcc gctgattttg gacggcgtat tgaagccggg cgtacaggtg aagaacgaag
    12661 ccgcttctgc ttcatggtgg cagaacgtat gggaaaaatc cctcggcaca ttgaaggggc
    12721 tggtcgaaat ccgccgtttg gaaaacaacg atgccatgct gatttctccc gaacaggcat
    12781 attttgtgcg tgaaaacctg cgcctccgcc ttttggatgc gcggactgca ttaatgcagc
    12841 gcaacagcga agtctatcag ggcgatttga acaatgccga agccgccgtc agacagtatt
    12901 tcgatgccaa gtctcccgcc acgcagtcgt ggctgaaaga actggcggaa ttgaaggcgt
    12961 tggatgtgcg catgacggcg gatgacggtt tgaaaaacag cctaaatgcc gtccgcgcct
    13021 atcgcgacgg tacgcgcatg acggcggcgg aaaatcaaga agcagaacag gcggcttccg
    13081 aaccggcaaa cgaaaacaca gcttccgaac cggctgccgc atcggatgtg aagaccatag
    13141 aagcaccgtc cctgccttcg gaacgcaaac cggaacagcc tgcaaaaaaa cagcccgtac
    13201 cggaaaaggc agggcgttcg ccgtccgcta aaggagaacg cgcatgaaaa cggtagtctg
    13261 gattgtcgtc ctgtttgccg ccgccgtcgg gctggcattg gcttcgggca tttacaccgg
    13321 cgacgtgtat atcgtactcg gacagaccat gctcagaatc aacctgcacg cctttgtgtt
    13381 aggttcgctg attgccgtcg tggtgtggta tttcctgttt aaattcatta tcggcgtatt
    13441 caatatcccc gaaaagatgc agcgtttcgg ttcggcgcgt aaaggccgca aggccgcgct
    13501 tgccttgaac aaggcgggtt tggcgtattt tgaagggcgt tttgaaaagg cggaactaga
    13561 agcctcacgc gtgttggtca acaaagaggc cggagacaac cggactttgg cattgatgtt
    13621 gggcgcacat gccgccgggc agatggaaaa catcgagctg cgcgaccgtt atcttgcgga
    13681 aatcgccaaa ctgccggaaa agcagcagct ttcccgttat cttttgttgg cggaatcggc
    13741 gttgaaccgg cgcgattacg aagcggcgga agccaatctt catgcggcgg cgaagatgaa
    13801 tgccaacctt acgcgcctcg tgcgtctgca acttcgttac gctttcgaca ggggcgacgc
    13861 gttgcaggtt ctggcaaaaa ccgaaaaact ttccaaggcg ggcgcgttgg gcaaatcgga
    13921 aatggaacgg tatcaaaatt gggcataccg ccgccagctg gcggatgctg ccgatgccgc
    13981 cgctttgaaa acctgcctga agcggattcc cgacagcctc aaaaacgggg aattgagcgt
    14041 atcggttgcg gaaaagtacg aacgtttggg actgtatgcc gatgcggtca aatgggtcaa
    14101 acagcattat ccgcacaacc gccgccccga gcttttggaa gcctttgtcg aaagcgtgcg
    14161 ctttttgggc gaacgcgaac agcagaaagc catcgatttt gccgatgctt ggctgaaaga
    14221 acagcccgat aacgcgcttc tgctgatgta tctcggtcgg ctcgcctacg gccgcaaact
    14281 ttggggcaag gcaaaaggct accttgaagc gagcattgca ttaaagccga gtatttccgc
    14341 gcgtttggtt ctggcaaagg tttttgacga aaccggagaa ccgcagaagg cggaggcgca
    14401 gcgcaacttg gttttggcaa gcgttgccga ggaaaaccga ccttccgccg aaacccattg
    14461 aaaccttcag acggcatcgt tgaaatgccg tctgaaaacc atttcacttg acaaacagac
    14521 tgacagaaaa cccgtatgac tcttttaaaa aacgatacct tcctccgcgc cctgcttaaa
    14581 cagcccgtcg aatacacgcc gatttggatg atgcgccagg cggggcgtta tctgcccgaa
    14641 tacaaagcca cacgcgcgaa agcgggcagc ttcctcgatt tgtgcaaaaa caccggattg
    14701 gcgaccgaag ttaccatcca acctttggaa cgcttcgatt tggacgcggc gattttgttc
    14761 tccgacatcc tgaccgtccc agacgcaatg ggcttgggac tgtactttgc cgaaggcgaa
    14821 ggcccgaaat tcaaacgcgc cctgcaacac gaagccgata tcgccaagct gcacgttccc
    14881 gatatggaaa aactgcaata cgtctttgat gccgtcagct ccatccgtaa agcactggac
    14941 ggccgcgtac cgcttatcgg cttctccggc agcccgttca cgctcgcctg ttatatggtc
    15001 gaaggcggcg gcagcaaaga attccgcacc atcaaaacca tgatgtactc gcgccccgat
    15061 ttgctgcaca aaatcctcga taccaacgcc caagccgtta ccgcctacct caacgcccaa
    15121 atcgacgcgg gcgcgcaggc ggtgcagatt ttcgacactt ggggcggcgt gttgagcgat
    15181 gcggcgttta aagaattcag cctcaaatac atccgccaga tcgtcgccgg actcaaacgc
    15241 gaaagcgaag gccgccgcgt gcctgttatc gtatttgcca aaggcggcgg gctgtggctg
    15301 gaaagtatgg cagaaatcgg cgcagacgca ttgggcttgg actggacgtg caacatcggc
    15361 gaagcacgcc gccgcgtcgg caagcaagtc gccctgcaag gcaacttcga cccgttcgcc
    15421 ctcttcggta cgccggaatc catccgcacc gaggtcgcac gtatcctagc cgactacgga
    15481 cacggcagcg gccatgtctt caacctcgga cacggcatca accaacacgc cgaccccgaa
    15541 cacgccaaaa tcttagtcga taccgtacac gagctgtctc ggcagtatca cggcgggtaa
    15601 gccggcagga aaccgcccga tatgccgtct gaaagccgag ggatggaaaa ttaggtaata
    15661 cggcaatatc cgccgtgtac ggatagtaca tgacggcggc ggtgttgccg cattggcgca
    15721 atcccaaccg tccctatttt cagacggcat ttttgttttc agatgcaggg aaaaccgatg
    15781 gcaaaaacgc ttaaaaccct ttaccaatgc accgaatgcg gcggcacctc cccgaaatgg
    15841 caaggcaaat gcccgcattg cggcgagtgg aacacacttc aggaaagctt ggccgcgccc
    15901 gagccgaaaa acgcccgctt ccaatcttgg gcggcggata cctcgaccgt ccaatccctc
    15961 tccgccgtta ccgccaccga agtgccgcgc aatccgaccg gtatgggcga actcgaccgc
    16021 gtattgggcg gcggtttggt cgatggtgcg gtcatcctgc tcggcggcga ccccggcatc
    16081 ggcaaatcca cgctgctgtt gcaaaccatc gccaaaatgg cgcaaagccg taaagtgcta
    16141 tacgtttccg gcgaagaatc cgcccaacaa gtcgccctgc gcgcgcagcg tttggaactg
    16201 cccaccgaag gcgtgaacct gcttgccgaa atccgcatgg aagcgattca agcagcgttg
    16261 aaacagcatc agcccgaagt cgtggttatc gattccatcc aaaccatgta ttccgaccaa
    16321 atcacttccg cccccggttc tgtgtcgcag gtgcgcgagt gtgccgccca actgacgcgc
    16381 atggcgaaac agatgggcat cgccatgata ctggtcggac acgtgaccaa agacggcgcg
    16441 attgccggcc cgcgcgtgct ggaacacatg gttgataccg tgctgtattt cgagggcgac
    16501 caacattcca actaccgcat gatacgcgcc atcaaaaacc gcttcggcgc ggcaaacgaa
    16561 ctgggcgtgt tcgctatgac cgaaaacggt ttgaaaggcg tgtccaaccc gtccgccatc
    16621 ttccttgcca gctaccgcga cgacacgccc ggttcgtgcg ttttggttac acaggaaggc
    16681 agccgcccgc ttttggtcga aattcaggca ttggtcgatg acgcacacgg cttcaccccc
    16741 aaacgcctca ccgtcggact ggaacaaaac cgccttgcga tgctgcttgc cgtattaaac
    16801 cgccacggcg gcatcgcctg tttcgatcag gatgtgtttt taaacgccgt cggcggtgtg
    16861 aaaatcggcg aaccggcggc ggatttggcg gtcatcctcg cgatgctttc cagcttccgc
    16921 aaccgcccgc tgcccgaaaa aatggttgca tttggagaaa tcggtttgag cggtgaagtc
    16981 cgccccgtcg cgcgcggaca agagcggctc aaggaagcgg aaaagctcgg ctttaaacgc
    17041 gccatcgttc ccaaagccaa tatgccgcgc aatgccaaag agtttccgaa cctgaaaatc
    17101 tacggcgttt cgagtttgca ggaagccatt gatatttgcc gcgacagcag ggaataaacg
    17161 gaaatgccgt ctgaaatcgg gtttcagacg gcatttggtt tgtggcggat tgaaacaaga
    17221 aggcataccg gcgacagata agatttgcgg caaagttgcc tgtgatgtgg caaaaacaca
    17281 cacgcccgtc atccccgcaa gggtgggaat ctggagtcgt ccgtttctgc aatgattgaa
    17341 aatcacggta acccaaccga ttggattccc gacttcgtgg gaatgagggg cgtgtgcatt
    17401 tgatttccat ccgccatatg tcggcgacgg gcttattcgc ctacggtttt ttgtatcagt
    17461 ttttcggcgt ttgccaaagt gtttgccact tcgtcaaaac cgatgcggct gccggcgatg
    17521 agggcgcgcg tatcggtata ggcggcgcgt actttgccgt ccgttttggt aacgaggacg
    17581 cgtaggggca gttgcagggc gaaggcgggg tctttgacca tcagcggcgt gccggctttg
    17641 ggcgtgccga agacgatgac ttttgccggc tgcatcgtta agccgtttcg gcgggcggct
    17701 tcctgatggt cgatgacggc aaaaatgtcc atccctttgc tttttatggc ggtttcaagg
    17761 cggctgacgg tttcgtcaaa actgtatttt gaggtgaggg tatgcgtggt catagcggtt
    17821 tcgttttggg tttgcggttc gctggcagga tgtgccgaag cggttgaaat gcagagtgcg
    17881 gatgcggcaa tcagggggag tatgtgtttc atcgtatttc cttttcggtt tgtttatcag
    17941 gcttcggacg gggcggcgcg cgccgcgccc gatgttgcgc cgcgtgccgg aacgccgtat
    18001 gccgtctgaa agcctgtcct ttcagacggc attgcgtcat ttcatccctt ttttgagcag
    18061 gtcttcataa ccgccgtgat tggcaacatt tgtataacct gcttttttca gttcttgaag
    18121 ggcggcttcg gcacgccgtc cgctgcggca gtagaggttg accggcgtgt ctttgtcggg
    18181 cgcggcttcg tgtatgcggc ggacgatttg gtcgacgggg atgttgaccg cgttgtgcaa
    18241 atgcccttcg ctaaattcct gttcggaacg gacatcgatc caaacgaccg gatgttgcgc
    18301 ggtttgggcg gcggataggg gtttttgcgg ggctgcctgc gcggcaaagg cggctgaggc
    18361 aatgagtgcg gtggtaatca ggtgtttgat attcataggg ttctcctgcg gttgttgtcc
    18421 gaaaggacgg gaagttattt tatctgttcc aaagcggcgg catctatgtc ccaacgccaa
    18481 acgccgccgc ctttgccatc caatccgcgc aaaaacaggt agcggacgga aacggcggcg
    18541 ggcggttgtc cgcgcagttt gaagtagcgt gcggcggcaa cggcgtaaat cagcgcctga
    18601 aggtaatagt ggtggtgcgc gacggcttcg tccatcgcct gccgcgtgta ggcggatgcg
    18661 tccgcgccga gatggtttga tttgtagtcg atgacgcaga tatttccctc ggggtcttgg
    18721 cagaccatgt cgataaagcc gtttaaaaag ccgttgacgg tgtggaagtc tagcgtttcg
    18781 gcagcggcac ggcagacttc gggcagcctg atgtcgtcgc gggcaaacca atcgcgtagc
    18841 cgtttgaggc cgaagtcttc ggtgtggagg gtaaagccca tttcgggaca gcggcactcg
    18901 ggcgggatgt cggacaggcc gtatgccccc gtcagcggcg ttttgcggca ggcttccgcc
    18961 atttcggcaa cggcgggcag ccatatttct tcaaatccgt attttttcag cttgtcggtg
    19021 atgagggttt cctgtccggc ggctgcttgt ccgaatttga aatcttcaag aatttcgtgc
    19081 aggcacagcc ccgcctgcgt gcctttcgga aagttgtgta tcgatatgcc atctgaagcc
    19141 gtcggcgttt cagacggcat cgcggctacc gagatttcgg cggcatccaa ggacgggcag
    19201 gcatcttctt cgccgccgtc gggcgtttgg gtatggcggc ttaaggcggt aaagctggtg
    19261 tggcggacaa atcggaatct gcgttcggga atgctgtttg cggcaaattc ggcggtttga
    19321 ccggcgttgc cgcgataggc ggcagggggc ggcgcatctt ctgtgaaggc gaaatttgtg
    19381 ccggaagggg cgttgtccgc cacgcgccgc cagttgcgtt tgagcatcgc gatgccgtct
    19441 ttttcacacg cataggcacg gcggacggtt tcacggctgt cttggggcga gccttcaatc
    19501 aggtaggcga gggggttgtc ggcagtattg gtggagtacg cggcgtagat gttgagctgt
    19561 tcctcggcac gcgtcagcgc gacataaagc aggcgcaggc gttccgccat ttcttcatcg
    19621 gcgtattgtt tctgttcgtc ttcggacagt tgcgcctttg ccaacagttc ggttcggttt
    19681 gcgctttggt ggaggatttg ccagtcggac ggcccggtat cttgcgcgtc ccacgcaaac
    19741 gggcagtaca ccagcggata ctgcaaacct ttcgaggcgt gcatggtaac gattttgacc
    19801 aaatcttcgt cgctttccag acggatggcg cggttgtcgc cgccgttgtt gccggccagg
    19861 ctgatttggt cgcgcagcca tttgtgcagc gcggcggggt tgcggttttg cgcgtcttcg
    19921 gcggcaagca gttcgagcag ttggaaatag ttggtcaggc tgcgcccgtt gttccggctt
    19981 aagaggcgtg tttcgatgcc gtgtgtttgg gaaaattgct gcatggcggc gaaaatgccg
    20041 tattgattcc agttgtcgag cgcggttcgg gcagattccg cccaatgcaa aatctcgctt
    20101 tcgttttggt tgaagtcgtg caattgctgc gcgtcataac cgaatatgcc gcttgttagg
    20161 acaaagcgca gcgttccggc gcggtgcggt tcgagccaga agccgatgag tgcggacagg
    20221 gcggcggctt cgggcgaggc gaacacggat tcgcgcgaaa gcaggacgct ttgcacctgc
    20281 cgttttttca gggcggcgga aaccatcacc gcctcgttgt gcgtgcgtac cagcacggca
    20341 atatcgcccg actgcaacgg gcagcctttg aaattcagac ggcattcggc ggcttcgttg
    20401 agcgcgtggg cgatttcgtc ggcgcaatag tcggcggcac ggcggcgcaa aacgtctttg
    20461 ttggcttttt cattgtcgtt ttcgtgcagc caacgaacct gtacggcagg acgtttgggc
    20521 gacaatctgc tttcggcacg cgccgcaccg acttccgaat agccgatgtt ttccaaaacg
    20581 aacgggcgtt ctttgaggcg gaacagcgcg cctatgctgc cgataagcgc ggcgtggctg
    20641 cggtagttgg tggcgagcgt gtagcggtgc cgcgcgtctt ccgccgcctg aaggtaggcg
    20701 taaatgtccg ctccgcgaaa gctgtaaatc gcctgtttgg gatcgccgac gaggaacagc
    20761 ggtcggtttt gggcgatgaa gattttttgg aagatttcgt attgcagcgg gtcggtgtct
    20821 tggaactcgt cgatcagcgc ggtttcccag ttttcggcaa cggcgcgggc gagggtttcg
    20881 gcgtgcggat tgtcggtcag cgcggtgtgg acatcgagca gcaggtcgtc gaaaccgcgt
    20941 tcgcggcgcg attttttcat ctcggcaagg ctgcggttga ggtattcgat taaatccagt
    21001 tgcagccgga tcattgttgc ttcttccgct tcttcgagtg cgttcaaatc gcgcccgaag
    21061 tctgccagtt tctgcaattc ggcaaatact gccgcatcgg gcgttttgcc ttttttcagt
    21121 ccggcttcga gtttgtcgga tgcaagtttc aagagtctgt cgtgtgtgtc tttgtccaga
    21181 aagggcagtt gtccggcggc ggatttttgt gccagttctt taaaaaggtt gccgaagctg
    21241 tttttgcggt aactgttgcc gttgaggtcg ggatggatgc gccaaaagcc ggcttccagc
    21301 tcgggcagca ggcggcagac ggtttgccat gaggtttcgg cgttgcgctg cgcctgtttc
    21361 aaatccgcct gcggacggcg gaaattcagg tacgggcggg acaggtaggc gcggatttgg
    21421 gcaaggacgg tttgcggcac agctttgcgt ttaaacgcca atgcggcaag caccggatca
    21481 ttgctgacgc gttcccgcca aaaatcttgc gccgggataa gcaggcggtc gccgtcttct
    21541 tcggtcagtt cgacatcgaa cggtgcttgg cacaagaagg cgtagtcgcg caggatgcgc
    21601 tggcagaagc cgtggatggt gtagatggcg gcgttgtcga attgcccgat ggcggccttg
    21661 aggcggacaa tcagacgcgt ccggccctct ttttgcaaag cctgttttaa gagtgcgggc
    21721 aggaaggtgt cgcctttgtg gtgttccgca caataggcgg cgatgccgtc tgaaagcgcg
    21781 ccgtctccaa gttcggcaat ttctttgctt tctaaaacct gcaacacatc gtccaaacgt
    21841 gcgcgcaggc gtgttttcaa ctcggcggtg gcggctttgg tgaaggtaac gaccaatacg
    21901 cgctcgacgt ttttttgttc taatacgatc aggcgcgtaa acagggcggc aatgccgtag
    21961 gttttgccgg tgccggcaga ggcctcaatc aggttggtgc cggcaatggg gacggttagc
    22021 gagtcgaatg cttggatggg tgcagacata gtgcgcgctc ggaaaacggt tggacggtat
    22081 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagt
//
LOCUS       DAXHTR010000013        20932 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.013, whole genome shotgun sequence.
ACCESSION   DAXHTR010000013 DAXHTR010000000
VERSION     DAXHTR010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20932
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.013"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(79..770)
                     /locus_tag="ACFNML_000365"
                     /pseudo
     CDS             complement(79..770)
                     /locus_tag="ACFNML_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(1076..1846)
                     /locus_tag="ACFNML_000366"
     CDS             complement(1076..1846)
                     /locus_tag="ACFNML_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391813.1"
                     /translation="MASIALYRLSFPVAAGGSGSRCTLCTKVHRESMLAIYSINILIY
                     NEKMFFRVTLYFVKNFVSFSKTRKSNEMMQKHKCLIDFILTVASQIAYADLPLSLEEL
                     LTDKGKFKLESSISYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQV
                     FDSQNVKAIQLSQAEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGA
                     ATAAGIGAATGVAAKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(1877..2035)
                     /locus_tag="ACFNML_000367"
     CDS             complement(1877..2035)
                     /locus_tag="ACFNML_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391814.1"
                     /translation="MKTPLPICTLSALAACALSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(2032..2874)
                     /locus_tag="ACFNML_000368"
     CDS             complement(2032..2874)
                     /locus_tag="ACFNML_000368"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6391815.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPMLVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKTQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            3112..4032
                     /gene="pyrB"
                     /locus_tag="ACFNML_000369"
     CDS             3112..4032
                     /gene="pyrB"
                     /locus_tag="ACFNML_000369"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFC6391816.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWGCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLDASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            4042..4500
                     /gene="pyrI"
                     /locus_tag="ACFNML_000370"
     CDS             4042..4500
                     /gene="pyrI"
                     /locus_tag="ACFNML_000370"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFC6391817.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            4565..5230
                     /locus_tag="ACFNML_000371"
     CDS             4565..5230
                     /locus_tag="ACFNML_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391818.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQMLAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALDLGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(5293..6510)
                     /locus_tag="ACFNML_000372"
     CDS             complement(5293..6510)
                     /locus_tag="ACFNML_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6391819.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFDRSE"
     gene            6694..7197
                     /gene="def"
                     /locus_tag="ACFNML_000373"
     CDS             6694..7197
                     /gene="def"
                     /locus_tag="ACFNML_000373"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFC6391820.1"
                     /translation="MALLNILQYPDERLHTVAKPVEQVDERIRKLIADMFETMYESRG
                     IGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYEEGCLSVPGIYDTVT
                     RAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVERLSQLKQGRIKTKLK
                     KRQKHTI"
     gene            7284..8210
                     /gene="fmt"
                     /locus_tag="ACFNML_000374"
     CDS             7284..8210
                     /gene="fmt"
                     /locus_tag="ACFNML_000374"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFC6391821.1"
                     /translation="MKVIFAGTPDFAAAALRAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVEQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQDVLDVPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIRPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGAKL"
     gene            8289..9548
                     /gene="rsmB"
                     /locus_tag="ACFNML_000375"
     CDS             8289..9548
                     /gene="rsmB"
                     /locus_tag="ACFNML_000375"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFC6391822.1"
                     /translation="MSMALAQKLAADSVAAVAEGRNLQDVLAEIRAAHPQLTAQENGA
                     LQDIAYGCQRYLGSLKHMLGQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNITAALQSHPPMTLRVNRRHGNAESYLEKLVAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            9514..10113
                     /locus_tag="ACFNML_000376"
     CDS             9514..10113
                     /locus_tag="ACFNML_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HFC6391823.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            10113..12230
                     /locus_tag="ACFNML_000377"
     CDS             10113..12230
                     /locus_tag="ACFNML_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6391824.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVLFSAMLL
                     LVLSAVLARYVILLLKDRRNGVFGSQIAKRLSGMFTMVAVLPGVFLFGISAQFINGTI
                     NSWFGNDTYEALERSLNLSKSALNLAADNALSNAVPVQIDLISTASLSGNMGRVLEHY
                     AGGFTQLSLYNAASGKIEKSINPHKLDQPFPDKEHWEKIQQTGSVRSLESIGGVLYAQ
                     GWLSAGTHNGRDYALFFRQPIPKDVAEDAVLIEKARAKYAELSYSKKGLQTFFLATLL
                     IASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTKL
                     FNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGRLKTFNKAAEQ
                     ILGMPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKILL
                     GKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAERL
                     AWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSSSLKLENQDLNALIG
                     DVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVKS
                     EAGRDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRIS
                     LSNQDAGGACVRIILPKTVETYA"
     gene            12223..13500
                     /locus_tag="ACFNML_000378"
     CDS             12223..13500
                     /locus_tag="ACFNML_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HFC6391825.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNCEVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDIAQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPASFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAGAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            13591..14778
                     /gene="dprA"
                     /locus_tag="ACFNML_000379"
     CDS             13591..14778
                     /gene="dprA"
                     /locus_tag="ACFNML_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFC6391826.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLHHFGSAQNALSAPAEQV
                     AALIRHKQALEAWRNAEKRALARQAAEAALEWEMRDGCRLMLLQDEDFPEMLAQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRYATPQAMRIAKDFGRALGGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDNPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAHGGILDKMGFGPVHPDVLAGQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            14804..15265
                     /locus_tag="ACFNML_000380"
     CDS             14804..15265
                     /locus_tag="ACFNML_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HFC6391827.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGLLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            15345..17651
                     /gene="topA"
                     /locus_tag="ACFNML_000381"
     CDS             15345..17651
                     /gene="topA"
                     /locus_tag="ACFNML_000381"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFC6391828.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     NPDQDFAMKYELIKRNAKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGV
                     KLEQFDLPNEAAQTDVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIADGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(17724..18419)
                     /locus_tag="ACFNML_000382"
     CDS             complement(17724..18419)
                     /locus_tag="ACFNML_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391829.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            complement(18439..18990)
                     /locus_tag="ACFNML_000383"
     CDS             complement(18439..18990)
                     /locus_tag="ACFNML_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391830.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHFHIQNGAVFFYSYRDV
                     TIVFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIIIYRPNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            19109..19486
                     /locus_tag="ACFNML_000384"
     CDS             19109..19486
                     /locus_tag="ACFNML_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiprotein-bridging factor 1 family protein"
                     /protein_id="HFC6391831.1"
                     /translation="METHEKIRLMRELNKWSQEDMAEKLAMSAGGYAKIERGETQLNI
                     PRLEQLAQIFKIDMWDLLKSGGGGMVFQINEGDSGGDIALYASGDVSMKIEFLKMELK
                     HCKEMLEQKDKEIELLRKLTETV"
     gene            19575..20144
                     /gene="rsmD"
                     /locus_tag="ACFNML_000385"
     CDS             19575..20144
                     /gene="rsmD"
                     /locus_tag="ACFNML_000385"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HFC6391832.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            20267..20518
                     /locus_tag="ACFNML_000386"
     CDS             20267..20518
                     /locus_tag="ACFNML_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HFC6391833.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDEEHPETHDELMAKYEKIIQFK"
     gene            20615..20698
                     /locus_tag="ACFNML_000387"
     tRNA            20615..20698
                     /locus_tag="ACFNML_000387"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20649..20651,aa:Tyr,seq:gta)
     gene            20729..20802
                     /locus_tag="ACFNML_000388"
     tRNA            20729..20802
                     /locus_tag="ACFNML_000388"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20761..20763,aa:Gly,seq:tcc)
     gene            20812..20886
                     /locus_tag="ACFNML_000389"
     tRNA            20812..20886
                     /locus_tag="ACFNML_000389"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20844..20846,aa:Thr,seq:ggt)
BASE COUNT         5247 a         5301 c         5694 g         4690 t
ORIGIN      
        1 gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat ataaaataaa
       61 tgggcaaaaa tcggtttatt atcgtttttg ccgcatttgg atttgttcta ccgtaaaacg
      121 tgtttgacga acgggattct tattaaaaaa catctgattt ctaacaaaat cagtattttt
      181 tggcacgatg gctaaaattt ttccttccat ttcgccatca cgtgttttcc atgcgctcaa
      241 gaattgtgat ttgctcattg agacgtgccc cagcgatgga tcagccagca aaacagtttc
      301 tccgttaata ccgttcaata ccgaaaaatg gttgttttta cggtatttta aatacacaat
      361 tacaggaatt tttagttgta ccaactgttc aaatggcaaa gcataacctt gtgcttcaaa
      421 acccagttcg ggcattatgc gttgcatatc atcaaaagaa gcacgcattt gggttttatc
      481 cattttgtcc aagatttccg cttcagaata atgtctgcca taaaaattat tcagtaacgt
      541 ggcaatcgaa gccgcgccgc aagaaaaatc caaatcttgt tttactatgc cggaatctcg
      601 ccgtgctttc caacttcgta catggatgtt ttggtaagaa gcggggggtc aacaaacata
      661 ggccaagcaa aaactatatt tggggcgaaa ccaatcaaag ccgcataatt tatcaattta
      721 taaagatttt ttatcataat atgtatacgc ggaataaacg catgaaacat aaaaaaataa
      781 aatcatatgc aattttattg catgaataaa tatgaataaa atagataatg atgggagtaa
      841 atacgccatg tattttggaa gtttaaattt attaataata aaataattta tcgtagcgca
      901 aataaatccc aaaattggaa cgattagaaa aaaaattaat acacccatca tcatgcaaac
      961 tctatattaa taaatagcta actaattcta ttgtggaaat tagttagcta actaaaagtt
     1021 attaatgatt atttccgaga attgactgca ttgttggcag cattggcccc aaaacctagt
     1081 gcatgaatac ccggtctcca tgccaaattc ccagccaatc cgcctccggc agcagcagcc
     1141 agccctgttt tgccgctact cctgttgccg caccgattcc tgccgcagta gccgcgcctt
     1201 gcgcagttcc taatttacca tgattataca aattagcacc atgatactcc catgcaccta
     1261 atgcaccgcc aaaagcagcg gctgcaataa tgggaacaaa ttcaccttgt gtttctttca
     1321 tttcagcctg tgataattga attgctttca cattttggct gtcaaaaact tggctgtcta
     1381 aattttgcgc cattacaggt gtaatcatca tagccattac agttgcaatt ttcgttgcgc
     1441 tggtttgcac ataaatagga ttagcaaatt cgctttgatt gcgttcagtg ttgatgtagc
     1501 taatactgct ttctagtttg aatttaccct tgtcagtcaa taattcttcc aaacttaaag
     1561 gtaagtcagc ataagcaatt tggctggcaa cagtcaaaat aaaatctatt agacatttgt
     1621 gtttttgcat catttcgttt gattttctag tttttgagaa tgatacaaag ttttttacaa
     1681 agtaaagagt cactctgaaa aacatttttt cattataaat caaaatattg atagaataaa
     1741 tagcgagcat cgattcacgg tgtactttag tgcaaagcgt acaacgcgag cctgaaccac
     1801 cagccgcaac aggaaaagaa agccgataga gtgcaatgct tgccaacgtg caagcgagct
     1861 tgcaagaacg cttggctcaa cgagagcagg caagacagaa agcagaaagc aggataggag
     1921 cggtaacgca aaggtctcgg gctttgattt cgccgtaaac cctgctgccg ccttgtccgg
     1981 aaagggcgca ggcggcgagt gccgacaggg tgcagatggg gaggggggtt ttcatttggg
     2041 gtcgcaacgg aagtggtatg cgcagatttc aaaaccgttt ttgaaataca ggcggtgcgc
     2101 gtcggcacgg tcgtggttga cgtggacgtt gaggtggatt ttggttaccc ctgtttccgc
     2161 gccgattttg cggacttctt ccaaaaggcg cgaggcgtag cctttgcggc ggctttgcgg
     2221 cagggtaacg atgtcatcga tgtggatgtg gcggccgctg gcgagggtgc aggcttcgcg
     2281 gaagccgcag acggcgacgg cattgtgttt gccttcttca aaaataccca gcaggcggta
     2341 gccttggggg cgttgggttt tgttgatctg ttcggtaaag cggttgatgt cggtcagggc
     2401 ggaacgcaaa acgctcaagg ctgcaaaggc ggtggcggtg tcgtccgcgc cgatttcgcg
     2461 caaaacgtag gatgcgcccg aggcggtctg ttcctgtgct ttctcggcgg cgtgtttttc
     2521 ttcgattgcc tgtgccagca tgacgtgttc gtcggcaggg ttgttttgtc cgccctgttc
     2581 gcgttcttcg agcagggctt tgcagtcgat gacgcgcagg tcgttgtcgg cggcaaagtc
     2641 catcaggaag cggaacattt gggggttgtc ggtttccagt tttttatcga cggcgacgca
     2701 gcggatgttg tccaccagca tggggcgcac ccatttcgaa taggaaagcc tgtggtcttt
     2761 ggtgaacgag gacagaatgc cgcacaggcg ttccgcccag tcgctgggac ggaaaatctt
     2821 gccggaactc gttgtgccgt ggatgacgac ttcgtagggg ttgcagacta acatggcggc
     2881 ttcctgaaaa gaaatgtctg gcgcgattat accttatgct tatgcgggcg tgtttggata
     2941 tgccgtctga aaagtacggg attcgtgcgg taaaactttg cggcggcaaa tgtgcgataa
     3001 tacgcgccgt attgccgctt ttgcgaagct gttccgcaaa catacgggcg gcgtggacga
     3061 cgtataaccg gatacccgcc tgacgcgggt tttttacgga agggggcaaa aatgcctaat
     3121 ccgctttaca gacagcacat catctcgatt tcggatttgt cgcgcgaaca gttggaatgc
     3181 ctgcttcaga cggcattgaa gctgaaggcg catccgcgcg gcgatttgtt ggaaggcaaa
     3241 cttatcggtt cgtgcttctt tgaaccgtcc acgcgcacga ggctgtcgtt tgaaacggcg
     3301 gtgcagcggc tgggcggcaa ggtcatcggc ttttcggacg gcgcgaatac cagtgccaaa
     3361 aaaggcgaga cgcttgccga taccgcccgc atcatttccg gctacaccga cgcaatcatc
     3421 caacgccacc ccaaagacgg cgcggcgcgc gtggcagcgg agttttcgcg cgtccccgtc
     3481 atcaacgccg gcgacggcac caaccagcac cccagccaga ccttgttgga tctggtaacg
     3541 atttatgaaa cgcagggacg tttggacaag ctcaaaatcg ccatggcggg cgacttgaaa
     3601 tacggacgta ccgtgcattc gctttgtcag gcgttgaaac gctggggctg cgaatttgcc
     3661 tttgtttcgc cgcccagcct cgcgatgccc gactacatta ccgaagagtt ggacgaagcc
     3721 ggctgccgat accgtatcct cggtagtttg gaagaagcgg cggaatgggc ggacatcctg
     3781 tatatgaccc gtgttcaacg cgaacgtttc gacgaacagg aatttgccaa aatccaaggc
     3841 aaattcaatc tcgacgcatc catgcttgcc cgcgccaaac cgaacctgcg cgtgctgcac
     3901 cctttgccgc gcgtggacga aatccatccc gatgtcgatg ctacgccgca tgcctattat
     3961 ttcgagcagg cgaccaacgg cgtttatgcg cgtatggcga tattgtcgct ggtgttgaac
     4021 gaagaagtgt gaggaaccga tatggaaacc ccgaaactca gtgtcgaagc cattgaaaaa
     4081 ggtacggtta tcgaccatat tcccgccggc agggggctga ccatcctgcg ccagttcaaa
     4141 cttttgcact acggcaacgc ggtaaccgtg ggcttcaacc tgcccagcaa aacccaaggc
     4201 agcaaagaca tcatcaaaat caaaggcgtg tgcttggacg acaaagccgc cgaccgcctc
     4261 gccctgttcg cccccgaagc ggtggtcaat accatcgaca atttcaaggt cgtgcagaag
     4321 cggcatttga acctgcccga tgaaattgcc gaagtgttcc gttgcccgaa cacgaattgc
     4381 gccggccacg gcgagccggt caaaagccgg ttttatgtta aaaaacacaa cgggcagacg
     4441 cggctgaaat gccattactg cgaaaaaacc tacagccggg attcggtggc ggaagcctga
     4501 cggattccct taaaccgagc gggcggcatt tcgtctgccg cttgttttgc caatctgaaa
     4561 tggaatgatg atgcacgctt ctgtccaaag ccgtttcgca ccgatacttt atgttttgat
     4621 tttctttgcc ggttttttga ccgcgcaaat ttggttcaat cagaaagcct atactgaaga
     4681 gctgcctccg cttctgtccg cattgtccgc cgtcgcgctg gtgtggctgg cgtgggcgtt
     4741 cgtgtcggcg cgttcaaagg ctaaggcgga aaagttctac cgcgaaaaaa tgatacagaa
     4801 cgaaagcata caccccgtcc tgcacgcttc tttgcaacac ttggaacaca agccgcaaat
     4861 gctcgccctg ctggtcaaaa accacggcaa agggatggcg gaacaggtca ggttcaaggc
     4921 ggaagtgctg cccgacgacg aagacgcgcg cacgattgcc gccgagttgg caaaaatgga
     4981 tatgtttgca ttggggacgg acgcggtcgc ctcgggcgaa acctatggac gcgtgttcgc
     5041 cgatattttc gagttgtcgg cggctttgga agggcgcgcg ttcaaaggaa tgttgaaact
     5101 gacggcggaa tataaaaaca tcttcggcga tgcctgccgt tcggaaacgg cgttggattt
     5161 gggcgcgctc aatcaggcgt tgcaggagat ttcaaaaaca tcggaaaagt ccaaacggat
     5221 attttattga agatggaaaa atgccgtctg aaacggaagg tgtttcagac ggcatttttg
     5281 tcggatgatt aattattcgg agcggtcgaa gccaaacttc acgcggctgc ggccctgatc
     5341 cggtatattg tccaaatcgc gtcccggatt ggcggcggtg tcgcctacgg aaatatcgga
     5401 gatgttttcc aaaatgatgg cggacgacag gtgttcggag gtgcggtaaa ccattgccaa
     5461 gcccacttct tcggcaggag tggaaatcag ctcgacggta tccctgcttt tgaaattgtt
     5521 ggagaggtcg acctgcatcg ttttcttgcg tttgtagagg ctcaaaaccg tgcctttgtc
     5581 caaaccgtcc gcctcgcctt tgtcgatggt gatggtttga aactggccgg caatccttgt
     5641 accttcaaac acggaaacga ttttagcctg aaccgggcgg gacggttcgt gcggcatcat
     5701 gttgaagcgg tcggtgtctt ccggcatttt catcaggtag tcgccctgct gtatttcgga
     5761 aatggcggtt tcgaccacca gcggctgtat cgaaggggtg cgcagcgggg taatcaaagg
     5821 atgggtgcgg gtatggtatt cgttgtcttt cggccgttct ccagcctgtt tcgagcgttg
     5881 ttcgaggaca gagtcggtat agtcgaggga gcgcacgatg ccgctgaatg cgacttcctg
     5941 cccgaggaat ttacccgtat ccggatcggt gatgttttta ttgattcggt aggtcaggta
     6001 gcggcccggc tctttcaggc ctttggtgta aaccctggtg cctttggtgt acagcagcct
     6061 gccttccggg cccgagagca ggcgcggcgc ggcagcggtt tctttgcggg aaacgatttg
     6121 cgggtgccgc ataaagatgc ggtagaagtt gacatcgatg gcgggaatac cgtatccgga
     6181 cacttcctta tccggactca ttttgacgac ggggatgccg tctgtctgtt ccaagccgag
     6241 gcgcggttcg ccgtcaacgt ggcgcaacac caatacctgg tccggataaa tcaggtcggg
     6301 attgtggatt tgatcccggt tcgcgtccca caggcggccc cattgccacg ggctgtacag
     6361 gtatttgccc gaaatgcccc acagggtgtc gccctgtttg accgtgtagc gttccggcgc
     6421 gttcgggcgc acctccaaat ttgccgccaa agtttgtgtt gagaatgcca tacctgccgc
     6481 gcagagcagg gttataatac gacgttgcat aaccgttccc cttatctgat aaatttcggt
     6541 ttgtcttgct tgattgggtt ggaaaaagcg gcggcagccc ctcgggatgt gccgcgtgat
     6601 aaaaaatgtt ccgcatttta acatcgaatt atccgcacca tcacggtaat tatgaaaaac
     6661 aggcggcgta tccgccgaag gaaagagaaa attatggctt tattgaatat cttgcaatat
     6721 cccgacgagc gtctgcacac ggtggcaaag cctgtcgaac aagtcgacga gcgcatccgg
     6781 aagctgattg ccgatatgtt tgaaacgatg tacgaatcgc gcggcatcgg gctggcggcg
     6841 acgcaggtcg atgtgcacga gcgcgtggtc gtgatggatt tgaccgaaga ccgcagcgaa
     6901 ccgcgcgtgt tcatcaaccc cgtcatcgtt gaaaaagacg gcgaaaccac ttacgaagag
     6961 ggctgcctgt ccgtgccggg catttacgac accgtaaccc gcgccgaacg cgtcaaggtc
     7021 gaggctttga acgaaaaagg cgaaaagttc acgctggagg cggacggctt gctggcgatt
     7081 tgcgtgcagc acgagttgga tcacctgatg ggcatcgtgt ttgtcgaacg cctgtcgcag
     7141 ctcaagcagg ggaggattaa gaccaagctg aaaaaacgtc agaaacatac gatttgaccc
     7201 ttttgccgtg ccgtctgaac gctgcaaagt tttcagacgg cacggtcttg tccgacaatt
     7261 ttacgcacac gcaggaacac gctatgaaag tcatcttcgc cggcacgccc gattttgccg
     7321 ccgccgcctt aagagccgtt gccgccgccg gttttgaaat cccgctggtg ctgacccagc
     7381 ccgaccgtcc gaaagggcgc ggtatgcaac tgactgcccc gcccgtcaaa caagccgcgc
     7441 tggaactcgg tttgcgcgtc gaacagcccg aaaaactgcg taacaacgcc gaagccctgc
     7501 aaatgctcaa agaggtcgag gcagacgtaa tggtggttgc cgcctacggt ttgattctgc
     7561 cgcaagacgt gttggatgtg ccgaaacacg gctgcctcaa tatccacgcc tcgctgctgc
     7621 cccgttggcg cggcgcggcg ccgattcagc gcgcgattga ggcaggcgat gccgaaaccg
     7681 gtgtgtgcat tatgcagatg gacatcggtt tggacaccgg cgatgtggtc agcgaacacc
     7741 gctacgccat ccgacctacc gataccgcca acgaagtcca cgacgcgctg atggaaatcg
     7801 gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag caaaggccgt ctgaacgcgg
     7861 tcaaacagcc cgaagaaggt gttacttacg cccaaaaact aagcaaagaa gaggcgcgta
     7921 tcgattggag cgaaagcgcg gcggttatcg aacgcaaaat ccgcgccttc aaccccgtgc
     7981 ctgccgcgtg ggttgagtat cagggcaagc cgatgaaaat ccggcgggcg gaagtggtgg
     8041 cgcaacaagg cgcggcaggc gaagtgttgt cctgttcggc ggacggtttg gtcgttgcct
     8101 gcggcgaaaa cgcgctgaag attaccgaat tgcagcctgc cggcggcagg cggatgaata
     8161 tcgcggcgtt tgcggcggga cggcatatcg aagcaggggc gaagctgtaa atcccttcag
     8221 acggcattcc gatccgcaaa cgggaatgcc gtctgaaacc atcagtcgaa gaaagcgaat
     8281 cacataatat gagtatggca cttgcccaaa aacttgccgc cgacagcgtt gccgcggttg
     8341 ccgaagggcg caatcttcag gatgttttgg cggaaatccg cgcggcgcat ccgcagctga
     8401 cggcgcagga aaacggcgcg ttgcaggata ttgcctacgg ttgccaacgt tatttgggca
     8461 gtttgaagca catgcttggt caaatgctga agaagccgat tgacaatccg cagctcgaaa
     8521 gcctgctttt ggcagcgttg taccaactgc attacacgcg caacgcgccg cacgccgtgg
     8581 tcaacgaagc tgttgaaagc atcgccaaaa tcggacgcgg gcagtaccgt tcgtttgcca
     8641 acgcggtttt gcgccgcttt ttgcgcgaac gcgacaagct tgcagcttcc tgtaaaaaag
     8701 acgatgtggc gaaacacaac ctgccgctgt ggtgggtggc ttacctgaaa aaccattatc
     8761 cgaaacactg gcacaacatc actgccgcgc tgcaatccca tccgccgatg acgttgcgcg
     8821 tcaaccgccg acacggcaat gccgaaagct atttggaaaa actggtggcg gaaggtatcg
     8881 cggctaaggc gttggacgaa tatgcggtta cgttggaaga agccgtgccg gtaaaccgcc
     8941 tgcccggttt ttcagacggc atcgtttcgg tacaggactt cggcgcgcag caggcggcgt
     9001 atctccttaa cccgaaagac ggagaacgga ttttggacgc gtgcgccgcg ccgggcggca
     9061 agacggggca tatcttggaa ctggcggatt gccgcgttac cgccttggac atcgatgcag
     9121 gccgtctgaa gcgtgtggag gacaatatcg cgcgcctggg ctttcagacg gcatcggcgg
     9181 cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg aaagcctttc gacgcaatcc
     9241 ttgccgacgt gccatgcacc gcttccggcg tggcgcggcg caatcccgac gtgaaatggc
     9301 tgcgccgtcc gaccgacgcg ctcaaaaccg cccgccagca ggaagccctg ctggacgcac
     9361 tttggcagac gctgaaacag ggcggcagaa tgctgcttgc cacctgttcc gtgttcgtcg
     9421 aagaaaacga cgggcaattg caaaaattcc tcaaccgcca tgccgatgcc gaaccgatcg
     9481 aatcgcgggt gcttttaccg aacaaacacc aagatggctt ttattacgcg cttattcaaa
     9541 agcattaaac aatggcttgt gctgctgccg atgctttccg ttttgccgga cgcggcggcg
     9601 gaggggatag atgtgagccg cgccgaagcg aggataaccg acggcgggca gctttccatc
     9661 agcagccgct tccaaaccga gctgcccgac cagctccaac aggcgttgcg ccggggcgtg
     9721 ccgctcaact ttaccttaag ctggcagctt tccgccccga taatcgcttc ttatcggttt
     9781 aaattggggc aactgattgg cgatgacgac aatattgact acaaactgag tttccatccg
     9841 ctgaccaacc gctaccgcgt taccgtcggc gcgttttcga cagactacga caccttggat
     9901 gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag tcctgaacaa aggcgcgctg
     9961 tccggtgcgg aagcagggga aaccaaggcg gaaatccgcc tgacgctgtc cacttcaaaa
    10021 ctgcccaagc cttttcaaat caatgcattg acttctcaaa actggcattt ggattcgggt
    10081 tggaaacctc taaacatcat cgggaacaaa taatgcgccg ttttctaccg atcgcagcca
    10141 tatgcgccgt cgtcctgttg tacggactga cggcggcaac cggcagcacc agttcgctgg
    10201 cggattattt ctggtggatt gtcttgttca gcgcaatgct gctgctggtg ttgtccgccg
    10261 ttttggcacg ttatgtcata ttgctgttga aagacaggcg caacggcgta ttcggttcgc
    10321 agattgccaa acgcctttcc ggaatgttca cgatggttgc cgtattgccc ggcgtgttcc
    10381 tattcggcat ttccgcgcag tttatcaacg ggacgattaa ttcatggttc ggtaacgaca
    10441 cttacgaggc gcttgaacgc agcctcaatt tgagcaagtc cgcattgaat ctggcggcag
    10501 acaatgccct cagcaatgcc gtccccgtcc agatagacct catcagcacc gcctccctat
    10561 ccggcaatat gggcagggtg ctggaacatt atgccggcgg gtttacgcag ttgtcccttt
    10621 acaatgcggc aagcggtaaa atcgaaaaaa gcatcaatcc gcataagctt gaccagccgt
    10681 ttccggataa ggaacattgg gagaaaatcc aacagaccgg ctcggtcagg agcttggaaa
    10741 gtataggcgg cgtattgtat gcgcagggct ggctgtcggc aggtacgcac aacgggcgcg
    10801 attacgccct gtttttccgc cagccgattc ccaaagacgt ggcagaggat gccgtcttaa
    10861 tcgaaaaggc gcgggcgaaa tatgccgagt tgagttatag caaaaaaggt ctgcagacct
    10921 ttttcctggc taccctgctg attgcctcgc ttttatcgat ttttcttgcg ctggtgatgg
    10981 cattgtattt tgcccgccgt ttcgtcgaac ccgtcctatc gcttgccgag ggggcaaagg
    11041 cggtggcgca aggcgatttc agccagacgc gccccgtgtt gcgcaacgac gagttcggac
    11101 gcttgaccaa gttgttcaac cacatgaccg agcagctttc catcgccaaa gaagcagacg
    11161 agcgcaacag gaggcgcgaa gaagccgcca gacattatct tgaatgcgtg ttggaggggc
    11221 tgaccacggg cgtggtggtg tttgacgaac aaggccgtct gaaaaccttc aacaaagcgg
    11281 cggaacagat tttggggatg ccgcttaccc ccctgtgggg cagcagccgg cacggttggc
    11341 acggcgtttc ggcgcagcag tccctgcttg ccgaagtgtt tgccgccatc ggcgcggcgg
    11401 caggtacgga caaaccggtc catgtgaaat atgccgcgcc ggacgatgcc aaaatcctgc
    11461 tgggcaaggc aaccgtcctg cccgaagaca acggcaacgg cgtggtaatg gtgattgacg
    11521 acatcaccgt tttgatacac gcgcaaaaag aagccgcgtg gggcgaagtg gccaaacggc
    11581 tggcacacga aatccgcaat ccgctcacgc ccatccagct ttccgccgaa cggctggcgt
    11641 ggaaattggg cgggaagctg gatgagcagg atgcgcaaat cctgacgcgt tcgaccgaca
    11701 ccatcgtcaa acaggtggcg gcattgaagg aaatggtcga agcattccgc aattatgcgc
    11761 gttcctcttc gctcaaattg gaaaatcagg atttgaacgc cttaatcggc gatgtgttgg
    11821 cattgtatga agccggtccg tgccggtttg cggcggagct tgccggcgaa ccgctgatgg
    11881 tggcggcgga tacgaccgcc atgcggcagg tgctgcacaa tattttcaaa aatgccgccg
    11941 aagcggcgga agaagccgat gtacccgaag tcagggtaaa atcggaagcg gggcgggacg
    12001 gtcggattgt cctgacggtt tgcgacaacg gcaaagggtt cggcagggaa atgctgcaca
    12061 acgccttcga gccgtatgta acggacaaac cggcgggaac gggattgggt ctgcctgtgg
    12121 tgaaaaaaat cattgaagaa cacggcggcc gcatcagcct gagcaatcag gatgcgggcg
    12181 gcgcgtgtgt cagaatcatc ttgccaaaaa cggtagaaac ttatgcgtag cagcgatatt
    12241 ttaattgtag acgacgaaat cggcatccgc gacctgctgt cggaaatcct gcaggacgaa
    12301 ggttattcgg tcgcattggc ggaaaacgcc gaagaggcgc gcaagctgcg ccatcaggcg
    12361 cgccccgcga tggtgctgct ggatatttgg atgcctgatt gcgacggcat cacccttttg
    12421 aaggagtggg cgaaaaacgg gcagctcaat atgccggtgg tgatgatgag cgggcatgcc
    12481 agcatcgaca cagcagtgga agccaccaaa atcggcgcgc tcgacttttt ggaaaaaccg
    12541 atttccctgc aaaagctgct gtccacggtt gaaaacgcgt tgaagtacgg tgcggcgcaa
    12601 accgaaacgg ggcctgtatt cgacaagctg ggcaacagtg ccgcgattca ggagatgaac
    12661 tgcgaagtcg aagcggcggc aaagcgcacg tcccctgtat ttttgacggg cgaagcgggt
    12721 tcgccgtttg aaaccgtggc gcgctatttc cataaaaacg gtacgccgtg ggtcagtccg
    12781 gcaagggtcg aatatctgat cgatatgccg atggaactgt tgcagaaggc ggagggcggc
    12841 gttttgtatg tcggcgacat cgcccagtac agccgcaaca tccaagccgg tattgccttt
    12901 attgtcggaa aggcggaaca ccgccgcgtc agggtggtcg catcgggcag cagggcggca
    12961 ggttcagacg gcattgcctg cgaggaaaag ctggcggaac tgctgtcgga atcggtcgtc
    13021 cgtattccgc cgctgcgtat gcagcatgaa gacattccct tcctgataca ggggattgcc
    13081 tgcaatgtgg cggaaagcca aaagattgcg cctgcctcat tcagtgaaga ggcacttgcc
    13141 gcattgaccc gttacgactg gccgggaaat ttcgaccaac tgcaaagcgt cgttgcaacg
    13201 ctgttgttgg aggcggacgg acaggaaatc ggcgcagggg cggtttcttc ccttttgggg
    13261 cagaatgtgc ctgccgaggg agcggaagat atggtgggcg ggtttaattt caacctgccc
    13321 ctgcgcgaat tgagggagga ggtggagcgg cgttatttcg agtaccacat cgcccaagaa
    13381 ggtcagaata tgagcaaagt ggcgcagaaa gtcggtttgg aacgcacgca cctttaccgc
    13441 aaactcaaac agctcggcat cggcgtttcg cgccgggcgg gggaaaaaac cgaagaatag
    13501 gcccggacgg ccggttttcc ggctgcgggc ttttgttttc agacggcatt tggtgcaaat
    13561 gccgtctgaa atcgtaaggg gacggatttt atgacagagg acgaacgttt cgcgtggttg
    13621 caactggctt ttacgcccta tatcggtgcg gaaagtttct tgctgctgct gcaccatttc
    13681 ggcagtgcgc aaaatgccct gtccgcaccg gcggaacagg tggcggcact gatacggcac
    13741 aaacaggcgc ttgaggcttg gcgcaatgcg gaaaaacgcg ccttggcgcg gcaggcggca
    13801 gaagcggcgt tggaatggga aatgcgggac ggatgccgtc tgatgctgct tcaggatgaa
    13861 gatttccccg aaatgctggc gcaggggctg accgcgccgc cagttttgtt tttgcgcggc
    13921 aacgtgcggc tgctgcacaa gccttccgcc gccatcgtcg gcagccgcta tgccacgccg
    13981 caggcgatgc ggattgccaa agatttcggc agggcgttgg gtgggaaagg cattcccgtc
    14041 gtgtcgggta tggcttcggg catcgacacc gccgcccatc agggcgcgtt gcaggcagaa
    14101 ggcggcacca tcgccgtgtg ggggacgggc atagaccgca tttatccgcc gtccaataaa
    14161 aaccttgcct atgaaatcgc cgaaaaaggg ctgattgtca gcgagttccc catcggtacg
    14221 cggccgtatg ccggcaactt tccgcgccgc aaccgcctga ttgccgcgct gtcgcaggtt
    14281 acgctggtgg ttgaagctgc gttggaatcc ggttcgctga ttaccgccgg cttggcggca
    14341 gaaatggggc gcgaagtgat ggcggtaccc ggttcgatag acaatccgca cagcaaaggc
    14401 tgccacaaac tgattaaaga cggcgcgaaa ttggtggaat gcctggacga catcctgaac
    14461 gaatgcccgg ggctattgca aaatacgggt gcttcatcat attctataaa taaggatacg
    14521 cccgacacgg gacgccgcgc cgttcagacg gcatacgccc cgccgcctgc cgcaaaaatg
    14581 ccgtctgaag cggcggcagg cggcacagcc cacggcggca tactggataa aatgggtttc
    14641 ggcccggttc atcccgacgt gcttgccggg cagcttgcta tgcctgccgc agatttgtat
    14701 gccgcactgt tggaattgga attggacggc agcgttgccg ccatgcccgg cggcagatac
    14761 cagcgtatcc gaacttgaac gcactttata ttaaggaaca cgaatgaccg aagtcatcgc
    14821 ctacctcatc gaacatttcc aagatttcga tacctgcccg ccgcccgaag atttgggact
    14881 gctgctcgaa gaagcgggtt tcgataccat ggaaatcggc aacaccctga tgatgatgga
    14941 agtactgctc aacagctccg aattttccgc cgaacccgcc gacagcggcg cattgcgcgt
    15001 gtacagcaaa gaagaaaccg acaacctgcc gcaggaagtg atggggctga tgcagtatct
    15061 gattgaagaa aaagccgtca gctgcgaaca gcgggaaatc atcatccacg cgctgatgca
    15121 catccccggc gacgaaatta ccgtagatac cgccaaagtg ctgaccctgc tgcttttatg
    15181 ggcaaacaag agcgagctac ccgtattggt cggtgacgag ctgatgagcg cactcttgct
    15241 cgataacaaa cctactatga actgaaacag gttcagacgg cattatcctg ctaacctgcc
    15301 gtctgaaata ccggaaccga gacatttatt accagaggaa acacatggcg aaaaacctat
    15361 taatcgtcga atccccatcc aaagccaaaa ccctgaaaaa atatttgggt ggcgattttg
    15421 aaatcctcgc gtcctacgga catgtccgcg accttgttcc caaaagcggc gcggtcaatc
    15481 cggatcagga tttcgccatg aaatacgagc tgatcaagcg caacgccaaa cacgtcgatg
    15541 ccatcgtcgc cggagcgaaa gaagccgaaa acatctacct cgccaccgac ccggataggg
    15601 aaggcgaagc catttcctgg caccttttgg aaatcctaaa atccaaacgc ggtctgaaaa
    15661 acatcaagcc gcagcgtgtc gtgttccacg aaatcaccaa aaacgccgtg ctcgatgcgg
    15721 ttgcccatcc gcgcgaaatc gaaatggatt tggtcgatgc gcaacaagcc cgtcgcgctt
    15781 tggactatct ggtcggtttc aacctctcgc cattgttgtg gaaaaaaatc cgtcgcggtt
    15841 tgagcgcggg ccgtgtacaa agtcctgctt tgcgcctgat ttgcgaacgc gaaaacgaaa
    15901 tccgcgcgtt tgaagcgcag gaatattgga cggtgcatct cgacagccac aaaggtcgca
    15961 gcaagtttac cgctaaactg gcccaataca acggcgtgaa actcgaacaa ttcgacctgc
    16021 cgaacgaagc cgcgcaaacc gacgtattga aagagcttga gggcaaagaa gccgtcgtta
    16081 ccgccatcga aaagaaaaag cgcagccgca atcctgccgc gccgttcacc acttctacca
    16141 tgcagcagga cgccgtgcgc aaactcggct tcaccaccga ccgtaccatg cgtaccgccc
    16201 aacagcttta tgaaggtata gacgtagggc agggcgcgat cggtctgatt acctatatgc
    16261 gtaccgatag cgtgaatttg gctgacgaag cgttaaccga aatccgccat tacatcgaaa
    16321 acaaaatcgg caaagaatac ctgccgagtg ctgccaaaca atacaaaacc aaatccaaaa
    16381 atgcccaaga agcccacgaa gcgatccgcc cgacttccgt gtaccgcacg cccgaaagcg
    16441 tcaaaccttt cctgagcgca gaccagttca aactctatca gatgatttgg cagcgcacag
    16501 tcgcctgtca gatgacgccc gccaaattcg accaaaccac cgtcgatatt accgtcggca
    16561 aaggcgtgtt ccgagtaacc ggacaagtgc aaaccttcgc aggcttcctc agcgtttacg
    16621 aagaaagcag cgacgatgaa gaaggcgaag acagcaaaaa actgcccgaa atgagcgaag
    16681 gcgataaatt gcccgtggac aaactctacg gcgaacaaca ctttaccact ccgccgccgc
    16741 gctacaacga agccacgctg gttaaagccc tcgaagaata cggcatcggc cgcccctcga
    16801 cctacgccag catcatctcc acgctcaaag accgcgaata cgttaccctt gagcaaaaac
    16861 gcttcatgcc caccgacaca ggcgacatcg tcaacaaatt cctgaccgaa cacttcgccc
    16921 aatacgtcga ttaccacttc accgccaaac tcgaagacca gcttgacgaa attgccgacg
    16981 gcaaacgcca atggattccc ttgatggaca aattctggaa accgttcatc aaacaagtgg
    17041 aagaaaaaga aggcatcgaa cgcgccaaat ttaccacgca ggaacttgat gaaacctgcc
    17101 cgaaatgcgg cgaacacaaa ctgcaaatca aattcggcaa aatgggtcgt tttgttgcgt
    17161 gtgccggtta tcccgagtgc agctacacgc gcaatgtcaa cgaaaccgcc gaagaggccg
    17221 ccgagcgcat cgccaaagcc gaagccgaac aggccgaact cgacggacgc gagtgcccga
    17281 aatgtggcgg tcgcctagtg tacaaataca gccgcaccgg cagcaaattc atcggctgcg
    17341 ccaactatcc gaaatgcaaa cacgtcgagc cgctggaaaa accgaaagat accggcgtcc
    17401 agtgtccgca atgcaaaaaa ggcaacctcg tcgagcgcaa atcccgctac ggcaaactgt
    17461 tttacagttg cagcacctat cccgactgca actacgccac ttggaacccg cccgttgccg
    17521 aagaatgccc gaactgccat tggccggtct tgaccatcaa aaccaccaaa cgctggggcg
    17581 tcgaaaaagt ctgcccgcaa aaagaatgcg gctggaaaga acagattgaa ccgcccgcgc
    17641 cgaaggagta agattaggtt ggtttgaaag agaaaaggtc gtctgaaaaa ttttcagacg
    17701 acctttgctt ttctgtgatt ggtttatttg aatccgcgtg ttgttttaaa gtccgataaa
    17761 atccggttca tttcaggcac aaacaaggcg atgtaatcgt aagatagacc gcgactggca
    17821 ctgggatggg gaaagcagac gacttcgcaa tcttcaaacg attggaattt gacattgaaa
    17881 cgtgtaccgt caaattcttt ttgcaccgtc tccagcggtt tggtctgctt accgaccaac
    17941 tgctcgaagc gtggcagtac attttggttg ttcagaaaat ccgccaacct gctgcccatg
    18001 aagaggatga ctttcggacg cagtttttcg atgtggtaga gaaaattatc gatgtgctcg
    18061 ggttgtgtga acttgtcggg attgtcgata gtgttgccct gtgtagcagc ccagttggtt
    18121 tgaaccaggg atttttcaaa tgcaccgccc aatccatttt cgtctaaggg gtgtccccac
    18181 atttcaaacc aattttttat cgtattgtcg taacgccact tttttgcctg ctctccgaaa
    18241 tagagggatt tgtttgcaaa tgtatggtcg attttgtttt cagggagttt gtattcacct
    18301 gctacataag cagcctcatc ggctttactc caaccccatt catagccaca aatcattaag
    18361 ccatgtttgt cgttgtagcc tttgaacagg ctgttgctca aattcaaatc cttcatcatg
    18421 aactcttcct tttaaaattt aagagcgatt gacttcaatg tttttagatg gggtggaaaa
    18481 atccttgtgt aggcaacata aattcaataa atttcttgat aattcgaaac ctactaatag
    18541 cgcacctata aaagcttttt cattacgttc agcatgacgg tcacgtcgtt catatttttt
    18601 acgcttgctg ttccctgtta ttacagctaa gccaagtgat atggcgagaa ttgcccaaac
    18661 aatagtactt aataacaatt ttccccatac tatcaatagg gaaagaaaaa accttttagt
    18721 attaggtcga tagattataa tccatgccca tgaaaatgca ataagagtta tacataagat
    18781 acaagctgat aggatttctt tatttttaat taaataactt agggcgatga ggatgacagg
    18841 tgtcaagaaa acaatagtta catcccgata gctataaaag aaaactgccc cattttgaat
    18901 gtggaaatgt gcacagaatc caatatagct aaggataata gttaatataa taaaaaaaga
    18961 ccaccaaggg tgaagagata ggaattccat gttttccctt tttttgtaaa aaggaaaaaa
    19021 tcttatctaa tagttaattg cctaaccagc cagaagaagt ttaaaatcta tcccaataat
    19081 tcaaccatct atacagaaag ttcagcttat ggaaacccac gaaaaaatcc gcctgatgcg
    19141 cgaattgaat aaatggtccc aggaggatat ggcggaaaag ctggcgatgt cggcaggcgg
    19201 gtatgccaaa atcgaacgag gcgaaacgca gttgaatatc ccgcgtttgg agcagttggc
    19261 tcagattttc aaaatcgata tgtgggactt gctcaaatcg ggcggtggtg ggatggtgtt
    19321 tcagattaat gaaggtgata gtggtggcga tattgcgttg tatgcgtcgg gtgatgtttc
    19381 gatgaaaata gaatttttaa aaatggagtt gaaacactgc aaagaaatgt tggaacaaaa
    19441 agacaaagaa atcgagctgc tccgcaagct gaccgaaacc gtttaaacag atatgccgtc
    19501 tgaaaaaagt tttcagacgg catattcttt gacaggtctt gtataatacc gtttgaactt
    19561 acaggttttt gattatggcg gcaggcaaac ataccaaaca cagcaaccgg gtacgcatta
    19621 tcggcgggca atgccggggc aggaaattga gtttcacatc cgccgacgga ctgcgtccga
    19681 cacccgacag cgtgcgtgaa aagctgttta actggctggg acaggatttg acgggtaaaa
    19741 cggttttgga tctcttcgga ggcagcggcg cactcggtat agaagccgct tcgcgcaacg
    19801 ccaaacgcgt gctgatttcg gataacaacc gccaaaccgt gcagaccttg cagaaaaaca
    19861 gtcgcgaact gggtttgggg caggtgcaaa tcgtcttttc agacggcatc gcatatttga
    19921 agaccgtatc cgaacagttt gatgttgtct ttctcgaccc gccgtttgca tggcaggact
    19981 ggcaaatcct gttcgatgcc ttgaagccgt gcctgaaccc ccgggcattc gtctatctcg
    20041 aggcgggtac gctgccgaat attcccgatt ggctgacgga atatagagaa gggaaatcgg
    20101 ggcagagtac attcgaatta agggttttcc aagtggctga ataatatgcg ctttgataat
    20161 catttccgag ttgtaaacat tcgtttgcaa ccgtccggtt caaaaaaacc ttgtgctata
    20221 atccgcgccc gcccggtttt gataatttag tggaaaagga aaagaaatgt cgctttttat
    20281 taccgacgag tgcatcaact gcgacgtatg cgaacccgaa tgccccaatg atgccatttc
    20341 ccaaggcgag gaaatttacg aaatcaaccc caacctctgc acgcagtgcg tcggacacta
    20401 cgatgagccg cagtgccagc aggtttgccc ggtggactgc atcctgattg acgaagaaca
    20461 tcccgaaacc catgacgagt tgatggcgaa atacgaaaag attatccagt ttaaataaat
    20521 tctttttaaa acatcaaatt atgtctgttt tgaaataaaa tcaaaaaaaa acttgacgga
    20581 aaagcaagcc gctaataaac taacgttctc ttttggaggg attcccgagc ggtcaaaggg
    20641 ggcagactgt aaatctgttg cgaaagcttc gaaggttcga atccttctcc ctccaccaaa
    20701 attcttactt ggggcagtag cgagtaatgc gggtgtagct caatggtaga gcagaagcct
    20761 tccaagctta cggtgagggt tcgattccct tcacccgctc caaacaatta ggcccatgta
    20821 gctcaggggt agagcactcc cttggtaagg gagaggtcgg cagttcaaat ctgcccatgg
    20881 gcaccatccc tcgattattc atttctttaa ggcttagata tataggatat tg
//
LOCUS       DAXHTR010000014        20829 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.014, whole genome shotgun sequence.
ACCESSION   DAXHTR010000014 DAXHTR010000000
VERSION     DAXHTR010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20829
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.014"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(84..1130)
                     /gene="recA"
                     /locus_tag="ACFNML_000390"
     CDS             complement(84..1130)
                     /gene="recA"
                     /locus_tag="ACFNML_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFC6391834.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEVANEIDAKIRALNGVEMHIT
                     EGTQDETDGERPEE"
     gene            1384..2148
                     /gene="aroD"
                     /locus_tag="ACFNML_000391"
     CDS             1384..2148
                     /gene="aroD"
                     /locus_tag="ACFNML_000391"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFC6391835.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLDQIKNLPFD
                     IVEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGAFPCSDDYYFKLL
                     DALIESRLPDIIDIELFAGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRAALDCLESGAD"
     gene            2168..4183
                     /gene="rep"
                     /locus_tag="ACFNML_000392"
     CDS             2168..4183
                     /gene="rep"
                     /locus_tag="ACFNML_000392"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFC6391836.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLPKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRHKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSQLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLLASEEGKAGEIKWRNVGDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGTWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(4251..5309)
                     /gene="dinB"
                     /locus_tag="ACFNML_000393"
     CDS             complement(4251..5309)
                     /gene="dinB"
                     /locus_tag="ACFNML_000393"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFC6391837.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYDSEVAKEIRAAIFAETGLTASAGIASNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMQTAGDLRRFERGELLNHFGR
                     YGYRLYDLARGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCASLLQAAQMLMARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
     gene            complement(5453..8541)
                     /locus_tag="ACFNML_000394"
                     /pseudo
     CDS             complement(5453..8541)
                     /locus_tag="ACFNML_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            8815..9594
                     /locus_tag="ACFNML_000395"
     CDS             8815..9594
                     /locus_tag="ACFNML_000395"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HFC6391838.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSIRFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYGHSFRFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYVMHRNRIPGQIMMENGF"
     gene            complement(9804..11189)
                     /locus_tag="ACFNML_000396"
     CDS             complement(9804..11189)
                     /locus_tag="ACFNML_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HFC6391839.1"
                     /translation="MIVTSRWPLLEKAFLRFGMPVAVVDLESTGGNLYEDRVTEVALV
                     KFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFAEIAGELFSVLKGCVLV
                     AHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSIIERLGIVVED
                     RHRAMADVSALCDYLEYSLSEHGVEAWIRQCFRLMNPKPLPAALPERLREQLYGLPDG
                     MGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWCNTEEVRFFPALGRLHAHK
                     VKAELVRHYGVDCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVTDNPPTGLFS
                     NKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACDRSDEQVLAF
                     AHKLPVLDWGKMHEVEITETDPLTGKTVVLHGMGGALEMDDGLWYFDKDLPDVFKAKF
                     KTDRKNIKEIG"
     gene            11307..11383
                     /locus_tag="ACFNML_000397"
     tRNA            11307..11383
                     /locus_tag="ACFNML_000397"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11341..11343,aa:Arg,seq:acg)
     gene            11401..11475
                     /locus_tag="ACFNML_000398"
     tRNA            11401..11475
                     /locus_tag="ACFNML_000398"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11434..11436,aa:Glu,seq:ttc)
     gene            12035..13210
                     /locus_tag="ACFNML_000399"
     CDS             12035..13210
                     /locus_tag="ACFNML_000399"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HFC6391840.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(13552..14100)
                     /locus_tag="ACFNML_000400"
     CDS             complement(13552..14100)
                     /locus_tag="ACFNML_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HFC6391841.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLSPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNAGSK"
     gene            complement(14103..15599)
                     /gene="ccoG"
                     /locus_tag="ACFNML_000401"
     CDS             complement(14103..15599)
                     /gene="ccoG"
                     /locus_tag="ACFNML_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HFC6391842.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRVSVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     AAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            16127..18106
                     /gene="tkt"
                     /locus_tag="ACFNML_000402"
     CDS             16127..18106
                     /gene="tkt"
                     /locus_tag="ACFNML_000402"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFC6391843.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPDNF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGIKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYVISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQAAVLPESLPRIAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPADLLFKAFGFTVDNVVDTVKSVL"
     gene            18247..19635
                     /gene="fumC"
                     /locus_tag="ACFNML_000403"
     CDS             18247..19635
                     /gene="fumC"
                     /locus_tag="ACFNML_000403"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFC6391844.1"
                     /translation="MSTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGRLDGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIRLLGDACNSFNEHCAVGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(19700..20623)
                     /locus_tag="ACFNML_000404"
     CDS             complement(19700..20623)
                     /locus_tag="ACFNML_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFC6391845.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYVGALVVVGGAVTAAVGDRLFKRR"
BASE COUNT         5130 a         5882 c         5067 g         4750 t
ORIGIN      
        1 caaaactcaa atcaagccgt tcggaggcgg ctcaaaaaaa cggtacttcg cagcagaagt
       61 accgtttatc gggatttcag gttttattct tcggggcgtt cgccgtcggt ttcgtcctgc
      121 gtcccttcgg tgatgtgcat ttctacgccg ttgagggcgc ggatttttgc gtcgatttca
      181 ttggcgactt cgggattttc cttcagccag acgcggacgt tgtctttgcc ctgaccgatt
      241 ttcgcgccgt tgtagctgta ccacgcgccg gatttgttga tgatgtcgtt tttcacgccg
      301 atgtcgatca attcgccttc ccaactgatg ccttctccgt agaggatgtc aaactctgcc
      361 tgacggaacg ggggggcgac tttgtttttg atgactttga cgcgggtttc gttgcccaat
      421 acttcttcgc cttttttgat ggatccggtg cggcggatgt cgaggcggac ggaagaatag
      481 aatttcagcg cgttgccgcc ggtggtggtc tcggggctgc cgaacattac gccgatcttc
      541 atccggattt ggttgatgaa cacaaccagc gtgttggttt ttttgatgtg tccggtcagt
      601 ttgcgcaaag cctgactcat caggcgcgcc tgcagtccga catggctgtc ccccatatcg
      661 ccttcgattt cggctttggg gacgagtgcg gctacggaat cgacgactac catatctatg
      721 ccgcccgaac ggacgagtgt gtcgcagatt tccaaagcct gttcgccggt atcgggctgg
      781 gacaggtaaa gctcttcgac ttttacgccg agtttgcggg cgtaaacggg atcaaaggcg
      841 tgttcggcat cgacaaaggc gcacacgccg ccgtttttct ggcattgggc gacggcttcg
      901 aggcagaggg tggttttgcc ggaggattcg gggccgaaga tttcgacgat gcgcccgcgc
      961 ggcagaccgc cgactccgag ggcgaggtct aatccgagcg atccggtgga aatgacttcg
     1021 aggttttctt cctgctggct gccgtccatt ttcatgatgg cgcctttgcc gaaacttttt
     1081 tcgatttgcg ccagtgcggc ggcaagggct ttgcttttgt cgtctgacat tggggttact
     1141 ccggaacaaa tgcggtatgt aggatgcggc gcaacacggg ctgcggcgcg ggatgtgtat
     1201 cgttttcccg atgtgcgggc tatcggtaat gctgcttcac gaggttgcca ttatcgcata
     1261 tttccttgct tgccgatatg cggcaggacg cggcggcttg tgccggaatg gaatctggat
     1321 gccgtctaaa aggcggccgg ctttgttata atggcggctg ttttttctgt gtgtgcctgt
     1381 tttatgtgtt cctgccttgt tgtcaaaaat accgttatcg gaagcggacg caccaaaatc
     1441 gccgtgccgc ttgtcgcccg cgatgccgcc gaactttccg ccgtacttga ccaaatcaaa
     1501 aacctgccct tcgacatcgt cgagttccgc gccgattttt tggaatgcgc gggcagcatc
     1561 ggcgaagtat tgcgccacac gcagaccgtc cgcgacgcgc tgcccgacaa gccgctgctg
     1621 tttacgttca gacggcatgg cgaaggcggc gcgttcccgt gttcggacga ttattatttc
     1681 aaactgctcg acgcgctgat cgaaagccgc ctgcccgaca tcatcgacat cgagctgttt
     1741 gccggcgaaa ccgccgtccg gcgcgccgtg gcggatgctc aaaaaaacgg catcgccgcc
     1801 ctgctctgca atcatgagtt tcaccgcacg ccgccgcaag aagaaatcgt atgccgtctg
     1861 aaacagatgg aggactgcgg cgcggacatc tgcaaaattg cggtgatgcc gcaaagcgcg
     1921 gaagatgtgc tgactttgct ttccgccacg ctcgaagcga aacggcttgc cgccaaaccg
     1981 gttgttacga tgtcgatggg gcagacgggt gcggtcagcc gccttgccgg acaggtgttc
     2041 ggctcaagca tcacgttcgg ttcgggaacg caaaactccg cgccggggca aatcggcgta
     2101 tccgccctcc gtgcggcact cgactgtctc gaaagcggcg cagactgatt tcagacggca
     2161 tcaaaacatg atgaaactca atccccaaca gctcgaagcc gtccgttacc tcggcggtcc
     2221 cctgctcgtc ctcgccggcg ctggcagcgg caaaaccggc gtgattacgc aaaaaatcaa
     2281 gtatttgatt gtcaatgtcg gctatctgcc gcataccgtt gccgcgatta cctttaccaa
     2341 taaagccgcc gcggaaatgc aggagcgcgt cgccaaaatg ctgcccaagt cgcaaacgcg
     2401 cgggctgacg atttgcacgt tccattcttt gggcatgaag attttgcgcg aagaggcgaa
     2461 ccatatcggt tacaaaaaaa acttctccat tctcgattct accgacagcg cgaaaatcat
     2521 cggcgaactc ttaggcggta cgggcaaaga agccgtattc aaggcgcagc accagatttc
     2581 cttgtggaaa aacgatttaa aaacgcctga agatgtcgtt cagacggcat cgaacatttg
     2641 ggaacaacaa accgcacgcg tgtatgcgag ctatcaggaa accttacaaa gctatcaggc
     2701 agtggacttt gacgacttaa tccgcctgcc tgccgtgctg ttgcagcaaa acagcgaagt
     2761 gcgccacaaa tggcagcggc ggctgcgtta tctgctggtt gacgaatgcc aagatacgaa
     2821 tacctgccaa tttacgttga tgaaacttct gaccggcgcg gaaggtatgt ttaccgccgt
     2881 cggcgacgac gaccagtcca tctacgcatg gcgcggtgcg aacatggaaa acctgcgtaa
     2941 aatgcaggaa aactatccgc agatgaaggt catcaaactg gagcaaaact accgctccac
     3001 cgcgcggatt ctcaaaatcg ccaacaaagt catcgaaaac aaccccaagc tgtttaccaa
     3061 aaaactttgg tcgcaattgg gcgaaggcga gccggtcaaa gtcgttgcct gccaaaacga
     3121 gcaacacgaa gccgactggg tcgtcagcca aatcgtcaaa caaaaactca tcggcggcga
     3181 caaaacccaa tatgccgatt tcgccgtgtt gtaccgggga aagcatcagg cgaggatttt
     3241 cgaggaagca ttgcgcggcg cgcgcatccc ctaccgtctc tccggcggac aaagcttttt
     3301 cgacaaagcc gaaatcaaag acgtgttgtc ttatgtgcgg ctgcttgcca accccaacga
     3361 cgatcccgcc tttctgcgtg ccgttaccac gcccaaacgc ggcatcggcg atgtaacgct
     3421 gggcaagctc aacacttacg cgcacgaaca cgaatgcagc ctgtatgaag ccgcgcaaaa
     3481 cgaagaagcc ctcgccacgc tgaacaatac caaccgccaa cacctgcaag cctttatgga
     3541 tatgttcggc agttaccgtg ccaaagccga aaccggcgaa gcgggcgagt tcatcaacag
     3601 cctgctcgaa gaaatcgact atgaaaacca cctgcttgcc agtgaagaag gcaaagctgg
     3661 cgaaatcaaa tggcgcaacg tcggcgattt ggtatcatgg tttgcgcgaa aaggcgggga
     3721 agacggcaaa aacatcatcg aactcgccca aaccgtcgcc ttgatgacgc ttttggaagg
     3781 aaaagacgaa gaagaaaccg atgccgtctc gctatccacg ctacacgccg ccaaaggttt
     3841 ggagtatccg tatgttttcc ttgtcggttg cgaagaaggc gttttgccgc acaacgacag
     3901 catcgaagaa ggcaacgtcg aagaagaacg ccgcctgatg tacgtcggca tcacccgcgc
     3961 caaacgccaa ctcacactga cccactgcgt caaacgcaaa aaacaaggca catggcagtt
     4021 ccccgaaccc agccgattca tagacgaaat gccgcaggaa gatttgaaaa tcctggggcg
     4081 caaaggcggc gaaccgattg tcagcaagga agaaggcagg cgcaaccttg ccgatataat
     4141 cggaaggctc gacaacctaa aaaaaagcgg cgcggcggat taaaccggag ccgcaatgcc
     4201 gtctgaaggc ttcagacggc atattttttg gacggcgcgc gtaaagcggt ttacgcccac
     4261 aaatcctgct gctggttttt cggcacaaga tgccccacgc cgataccgat aaggcggaac
     4321 gcgtcttccg tctgcggcgg gacgcgcgcc atcaacattt gcgccgcctg aagcagggag
     4381 gcgcagtcgg gcaatacgga ggaataagtc agcgtgcgcg tgatgatgcg gaaatcgtag
     4441 gtcttcagct tgagcgttac actttgggct tcgacgtttt tgcgcgtgat ttgccgccac
     4501 aagtcttcgg caagatgggg caagtgtccg gcagcctgct cgagcggcag gtcttcgggc
     4561 agggtgattt ctgtggagat ttggaggcgt tcgcgttcgg ctttgacggg gcgttcgtcc
     4621 gtaccgcgcg ccaaatcata gaggcggtag ccgtagcgtc cgaaatggtt taagagctcg
     4681 ccgcgctcga aacggcgcaa atcacccgcc gtctgcatac ccagcgactg catttttttc
     4741 agcgttacct tgcccacgcc ggggattttg cccaaaggca gggtttccaa aaatgccatg
     4801 actttgtgcg gcggcaacac aaactgcccg ttcggcttgc gccagtccga cgcgattttc
     4861 gccagaaatt tgttcgacgc gatgcccgcg gatgcggtca aacccgtttc cgcaaaaatg
     4921 gcggcacgga tttctttggc aacttcactg tcgtaaggga tgtttttgaa attacgggta
     4981 acgtcaagat aggcttcgtc cagcgacaag ggttcgatta aatcggtata acgcctgaat
     5041 acggcgtgaa tctgcgcgga aacctgacgg tacaaatcga aatgcggcgg cacatacacc
     5101 gcttgcggac acagcctttt cgccgttgcc accgacatcg cggaatgcag cccgaactgc
     5161 cgtgcctcat acgatgcggc gcaaatcacc gaacgcgcgc cctcccacgc gacgaccacc
     5221 ggccgccctt tcaaatgcgg ctgttcgcgc agctctaccg atgcgtagaa tgcgtccatg
     5281 tcgatgtgga taattttgcg tgaagacatc ggctcttctg aggataaaag ggatattcta
     5341 ctgccggcat cgggcaaatt ccaaatatac gccccgatag gcctgcctcc ataaaaatgc
     5401 cgtctgaaac ataccctgtt tcagacggca tccgcaaaac tacggttttc aattaaaact
     5461 gccaatccag tttcatgctg acagtgcgcg gttctccata gaagttgttt gcgccgcgcg
     5521 tacggttgta gttgttctca aaataagtgc gtccgtttaa gttcgtaccg atgaggctca
     5581 atttagcgtg tttgcccaat tcgtaacgga cgaaaccgtc tatcagcccg tagccgccct
     5641 gcctgatgtt gtacaggctg cttgtgccgc tttgtgcgga cacgccgccg ccgacggtca
     5701 gccccgtatt cggtatatgg aagctcgttc cgaaacggaa tatgtgcacg ggtgtgaaat
     5761 tgctgaagtt gtacgggtct gcgcctgtgt ttttggcgag gcgttcggcg ttgacttcgg
     5821 cggcgttttt gtagcggctc ttgttgtagg tgtaacccgc aaagactttc caatcttcgt
     5881 tcaactcacc cgacaactcg gcctccgccc cacggctgac cactttgcct atcggtttgg
     5941 caacggtttg gaacgacccc tgcttgccgc ctgctccggg aacatagcca aaatcgacga
     6001 ccgtgcggtt tttctgttcg aggtaaaaca atgcgaacga agcattcagc cgtccttgca
     6061 agaatgcgcc tttccagcct acctcatagt ttgtgccgac caaaggcggc aaaacggttt
     6121 tggcactgac atcgacatta tcctgctgtt tgaagatttt ggtataactt ccgtaaatac
     6181 tctgttgcgg tgtcaagtca taggtgatgc ctgcataggg cgtcaattta tgaccttgca
     6241 tcttggccgt gtaatggtcc tgatccgcct taatactcga tgctgtctga aaatcgcttg
     6301 ccggctgccc atagcggaca ggcatatctt tggtttgcga ggtttcatag cgcgtgtagt
     6361 gcagcccgcc caaaaggtgc agtcggccgg ttacgttgaa acgcgtgctg gcagtcagcg
     6421 aatgggtttt gttggtgttg aggtatttgg cgtagttata cagcgcagga acatggtcat
     6481 cggccacttt aacagttttc caaaccggca ccgtaccgga aaaaccggta aagacaggcg
     6541 agccgtcggg attggtctcc tgaatcttgt tgcctttttc gtccagctca tatacatcga
     6601 catataccgg tgtccggctg ccgctgtatt cgtcatagta atacacctgc ttgccttcgg
     6661 catcgagctt gggctcggtt tttattttct tggcattcct gcattcttcg gcataaacgg
     6721 tacggttgcc tttttcatcg tacgcctgcc aatcgggttc tttatgcccc ctgaccaaag
     6781 gagaagacaa atcaccgtcc ggctcccctg acaacttccc gcatacacgc cgtgcgttgc
     6841 ccctgtattc ggacgtactc tgtggcggcg ttcgtagatt tccagatatt cggaacgtat
     6901 cttttcatcg ccgtaggcat agccgacaaa gaaatcatgc tcccgcccga acagcccata
     6961 tgtgccggtc aggtcaagtt taattcccca ttggcggtcg tctttggtat gccgcaacgg
     7021 catataactg tatcgtcggt tggcggtagc cttccgatta aaggaagagt catacaggct
     7081 gtttggaaaa cgttgtgccg cattattaaa gatgccttcc ttcgcaaggg ctttatcgac
     7141 aaattccgac ttgtcggcat caacacccgg atccccccaa gaaccttgac agataaagtc
     7201 cagcgcaaaa ggggcactca tacactcgtc aaaaccggct ttgcgttctg cggcacggcg
     7261 gctgcggtac tgctcgaaag cagtattatc gaaacggttt ttaacaaaat cgtctttgcg
     7321 ctcacggtat tccttggcgg tttcgtcacg atatgctttc agtttctcca atgccttatc
     7381 tttcggctcg aacgggatga cttcgttttt ttcggtcaaa aagcctaccg catcctcatc
     7441 tgacaaaccc gccgcatgtt cgtttttcag aaaaaactgc cccaccttcg catcggattc
     7501 attcttggta taagacactt cggcattgag ctgccaaccg ttgtcaaaca catgtttgaa
     7561 acctgagaaa aggttgtatt tgtcagcact caaccgcgac caatcctccc ccaaataagt
     7621 gttgcgcggc agttgcaaag gccggttgca ggcaggcgtt gaactgaacg gggcagtttt
     7681 ctgattttca caggacaaaa taatgcccga aaaatcagga acctccctac tcttctgata
     7741 catgccgccc aaagtaagca cactgctgtc gcccgcatcg gcttcggcaa tgccgtaaac
     7801 catatgtttc ctgccccaaa cgcggtcttt aaacgatttt ttatactctt ccgcacccac
     7861 caaccttccg cgtaaggtat tcgccttatt caggctgcct gaaacatcca acactgcacg
     7921 ccggctgccg cgatggtcgg cggtcagctc tccggtatgt ttgaaagaag cggtaggtcg
     7981 cttacggatc aaattgacgg ttccacccgg ctctgaattg gattgggtca accccgttgc
     8041 accccgtaca acttcaatat ggtcataaac cgccaaatcg gtactcggag acacgtcgat
     8101 tttcgccgta tatcccgaac ggcctgcaac attgacggta ataccgtctt caccaatctg
     8161 atcaatatag aaaccgcgtg acaaaaaccg cgtctgcaag cctgaatcgc gcacaacgtt
     8221 gacacccgtc gtgtttttca ttgcctcttc aagcgtatgc accgccttat cgtcaaggcg
     8281 gctgcgcgtg atgacgctga ccgactgcgg cgtatccttg cccgcaatcc tcatacctgt
     8341 ggcggtggac atccgatcta tcgtataaga acgggtcttc tcggtcttgc ccgacagagc
     8401 atgagagccg cgtacattga ccgtatccag actgacggta ttgccgtctg aaacaggcac
     8461 aacaccgtct gcaaaagaac caccgtaagc cgataacagc ataacggtca gaattttaag
     8521 tgaaaaatga ttttgattca tagagacctc tgtaatatgc aagtgtgcaa atcgtccaaa
     8581 ggccctcaca actgttttga ttttttatat taattgaaaa aaagtaattc tcaattaaat
     8641 ttatagatgg gattgtattc ccattttgac aaaaaacaaa ctgctcctta ccgtttattt
     8701 caaaaaacga taatattgta ttgaaaaata tccgaattta aatacagatc gtcaatgcgg
     8761 aaaaaaacac ccaaattggc tataatcccg acaaacacac tcaaggacaa caacatggca
     8821 gcctcgcccg aagcaaaatt cactgaagaa aagattttgt gggtcaaaca ccacacgccg
     8881 aaactcatca ctttcgccat cagccgtcca gaatcctacc gttttaaagc cggacagttc
     8941 tcccgactcg gtttctacga aggggaaggc tttatttggc gtgcctattc catcgtttcc
     9001 gcagaatatg ccgacacgct cgaatatttt gccgtactca tccaagacgg ccccatgtcg
     9061 atccgtttcg ccaaaatgca acagggcgac accatcctgc tcgataaaaa tgccaccggc
     9121 ttcctcctgc ccgaacgctt ccccgacggc aaggatttgg tgatgctctg caccggctcc
     9181 ggcatcgccc ccttcctttc cattctcgaa caacccgaaa tccggcagcg tttcgatacc
     9241 gtcaacctga tacattccgt atcttttccc gaagaattga ttttcaacga ccggctcgcc
     9301 gcattgtccg aacaccccct ggtaggcgaa tacggacact cgttccgttt cgtccctgtt
     9361 accacccgtg ccgccaaccc ctcgggctta agcggaaaac gcattccgga actcttaaaa
     9421 aacagcagca tcgaacaggc gctgcatacc aagttcaccc cggaatccac acggtttatg
     9481 atttgcggca acccggaaat ggtcaaagac actttccaaa cgctgctcga catgggttac
     9541 gtcatgcacc gcaaccgcat tcccggtcaa atcatgatgg aaaacggctt ctaaaaacca
     9601 ccctgcttgt ccgatgcctt cggatggaag ggcaaaccga cacggcacga aaaccgcgtc
     9661 ggtaaaaatg ccgtctgaaa aaattcagac ggcatcttcg gacacattac ctgaaacggt
     9721 aacacattcc aacccaaacc gatcaggcaa tcaatacgaa cgactgttta catacttacc
     9781 gactttcacc aaccggtatt gatttaaccg atttccttaa tatttttcct gtccgtttta
     9841 aacttcgcct taaacacatc cggtaaatct ttatcgaaat accaaagccc gtcatccatt
     9901 tccaatgcgc cgcccattcc gtgcagaacg actgttttcc ccgtcagcgg atcggtttcg
     9961 gtaatttcca cttcgtgcat tttgccccaa tccaaaacag gcaacttgtg tgcaaacgcc
    10021 aaaacctgtt cgtcagagcg gtcgcacgcc ttatcgcatc ggcctgaaac atatacggga
    10081 caaggctcat cctctgcata accgtccgga aggataccgg ctgaggcagg acacaatccg
    10141 tatgtttccg cccacgacga caaagcccgt ctcgccgcct ttttattgga aaataatccg
    10201 gtaggcggat tatccgtcac accgtttttc aaagccgctg ttttcgcatt caacatgccg
    10261 tctgaacctt tttcaaacct gacggtcgta aatgttttaa gcagattttt ggcagatacg
    10321 taacaatcca ccccataatg cctgaccaat tccgctttaa ccttatgggc atgcagcctg
    10381 cccaatgcgg gaaaaaaacg gacttcctcc gtattgcacc aatcaaacgg ggcttttccg
    10441 gagtccaata aagccgaaac ctcgctatat acccgttcaa acgtaccgat gtaatttact
    10501 ttccctccgc cgtcgaaaca ggccagcacc cccataccgt caggcaaacc gtacaactgt
    10561 tccctcaacc gttcgggcag cgcggcaggc aacggtttcg gattcatcaa acggaaacac
    10621 tgcctgatcc atgcctcgac cccgtgttcc gacagactgt attccaaata atcgcacaat
    10681 gccgatacat ccgccatcgc acgatgcctg tcttccacaa caatccccaa cctttcgatg
    10741 atactgtcca ggctgtgctt gtaaaattgc ggatacagac accgggacag ttgcacactg
    10801 cacaaagcag gcgatgaaaa tccgataccc gcacgatgaa actcatgctt taaaaacgta
    10861 tagtcgaaac ggctgttatg tgcaaccagc acacaaccct tcaataccga aaacaactcg
    10921 ccggcaatct ctgcaaaaac aggtgcgtcg gcaaccatgc cgtctgaaat ccccgtcagc
    10981 cccgccacaa actgcggaat cggtttttga ggattaacca accactcatg cctcaccacc
    11041 cttccctgct caaacttgac caaagccact tcggttaccc tgtcttcata cagattgccg
    11101 cccgtcgatt ccaaatcaac cacggcaaca ggcattccaa accgtaaaaa tgccttttcc
    11161 agcaagggcc aacgggaagt aacaatcatt ttattctctt taaattcaaa caacaaaccg
    11221 atattttaca cttttgaggc atttcatcca acaaaacaat tgacagaatc cgatgattac
    11281 cctaaaattc aaatctttct tgcagcgcac ccgtagctca gttggataga gtatctggct
    11341 acgaaccaga gggtcgggcg ttcgaatcgc tccgggtgcg ccagtaagaa aatacaatat
    11401 gcgcccatcg tctagcggtt aggacatcgc cctttcacgg cggtaaccgg ggttcgattc
    11461 cccgtgggcg tgccaaattc caaatccccc gaaataatcc ctcggggatt ttttattgcc
    11521 tcggcaattc attaccatat ctttacctac ccccttcatc agactctcag acgtaatcga
    11581 atcatattca aacctttgcc gtgcaaaccg atatcacgta accggatgcg gtgtccgtcc
    11641 aacattttac ccgattgaaa cgcctgatat attgcacccc atcaacgtgg cattactttt
    11701 cttaacaatc ccctttgaca gcaactgact agggcttttt tatgccatca tcaaatttat
    11761 agtggattaa ctttaaacca gtacggcgtt gcctcgcctt tccgtactat ttgtactgtc
    11821 tgcggcttcg tcgccttgtc ctgatttttg ttaattcact ataatatttt ctctcccgat
    11881 tgaaacaggc gtaacagaat atgcccgaag ctccggctgc tttcttgttt accgccgcga
    11941 tatttagagt ataataccaa atttgagcaa tagttctaaa acagttagaa ccatttttca
    12001 tgagcctgac tgattcgtat actcggagaa actgatgcaa aatatttttg accctttggt
    12061 tattcgtgga aaatccctta cccccatcgt gcaaggcggt atgggggtcg gtgtttccgc
    12121 atcgggttta tccagcgcgg tggcacgtga aaacggtatc ggaacgattg ccagtgtgga
    12181 tttgcgccac cttcacgaag acctactcgc cgaatcacaa atcaatccga gtgaagagaa
    12241 atatacatct ttgaactgta ccgcattaga cagggaaatc caaaaagcca aaagcgcttc
    12301 agagggaaaa ggactgattg cggtcaacgt gatgaaggcg gtcaaagacc acgccgcgta
    12361 tgtccgccag gcttgcgaat caggggcgga tgcggttgta atgggtgccg gcctgccttt
    12421 agacctgccg gaaatgaccg agggctatca taaagatgtc gcgctgctgc cgattctgtc
    12481 cgaatcgcgc ggtattaata tcgtcttgaa acgttggatg aaaaaaggca tattgcccga
    12541 tgcgattgta gtcgaacatc ctgcccacgc ggccgggcat ttgggcgcgt caaccgtcga
    12601 aggcgtaaac gatgctaaat tcgacttcaa acgcgtgatt gaggaaacgt ttgaagtttt
    12661 caaaagttta gggctggaaa gcgaaaaaat cccgcttatt ttggcaggcg gtatggcaaa
    12721 ctttgaaaaa gtcaaaaccg ccctgaaaaa ctggggagca tccgccgttc aaatcggtac
    12781 ggcttttgcc gttaccgaag aaggagatgc gcaccttaac ttcaaaaaaa cgcttgctgg
    12841 tgcggaaact gaaaaagtag tcgaatttat gtctgttgcc ggtttgccgg cgcgcggcgt
    12901 acgtaccaaa ttcctagaca gctatatcag gcgcgaaggc aaacttcagg caaacgccaa
    12961 agccgacccg cgccgctgca cccaaggatt aaactgcctg accagctgcg gcctgcgcga
    13021 cgggctttcc aaagcagggc aattctgtat tgatatccag cttgctgccg cattccgtgg
    13081 agaagtggat aaaggcctgt tcttcagagg taaagaccct cttcctttcg gcaatgccat
    13141 ccgcaccgtc cgcgagacga tacaatatct gctgacgggg agcgaacctg ttgcaacgct
    13201 cggacgctga catcaggtag gatttgattt gcaaacaaaa tgccgtctga agggccttca
    13261 gacggcgttt ttcaggctgc gtttcggaat agtgtcgaaa aaacggagag aaataaaaac
    13321 gtttttatgt aaacaaaacg ccttccgcat tgtgtcgggc atacaacaca aatgccgtct
    13381 gaagcccttc agacggcatt tttcaggctg catttcggaa tggtgttaaa aaataaacgg
    13441 gataagatac atttattttc gtccgataaa tcagaccatc acgccgaata atcaaacaat
    13501 gcctgcccga cattgcatct ggcaaaccaa tgcaatgaaa acacggcttt tttatttgct
    13561 tccggcatta ttgaaaagtt tatccatcgg ggtcagatcc accgcattgc cttggctggt
    13621 aatccatttg ttctcgacgc gccacacgcc tgccgcgtcc tccacgcgca cataccagtg
    13681 gttggtcggc gaaagggttt tgaacaccgc ctcatattcc gccctgccgt tctgcgcgct
    13741 gccgacgggc ttgagggcga cggtttgatc gtccgccttg cgggtcgggt gcatcagcag
    13801 caggttcaaa ggctgtttgc cgtcaaactc gccgccgaca aacacttttg ccgcattcat
    13861 atcgggggaa atgagaacct gcaccccgat atgccgtctg acggcttctt catcccgatg
    13921 aagctggatg tcgatatgtt tgccgtcttt ataataatcg tccgtaacca aatctgtcgc
    13981 gtgctgctgc gcgacaaaaa acatagcgac gctggcgatg acgacaaaaa tcggccccgc
    14041 catcaagatc cacggccaga cgtgtttgta ccaaggtttg attggagtgt tttgagacac
    14101 ggttattctc cgataaaggt tgcatcttct tccaagacga ccggcttgcc gtcgggcgcg
    14161 ccgctttcgc ggtattggaa ggtaaattcg atagggtggc tgcctttgtc cgcgtattcc
    14221 ggaatggtgg acacttggac gggaagggtt accgtttcgc gcggggcaac cttgataccg
    14281 ccttcgggca gcccggtcag ggcgatttcg tcaaagcctt tgacacttgc ggtaatgagc
    14341 tgttcttttt cacttttgtt gatgatacgc aggctgtatg cgttttccag ccagcctttg
    14401 gcgttttcgc gcaccagtac gccacggtct ttcaaaatat cgacctcgac cattttgcgc
    14461 gtggacaaac cggccaggaa ggcaatgata actaacgcca acaccgcgcc gtaacctgcc
    14521 acgcgcggtc tgagcagccg ttttttaatg tctttttcag aatattcgtg ttccagcgcg
    14581 ctttcggtcg tataacggat taatccgcgc ggatagccca ttttgtccat aatctcatcg
    14641 cacgcgtcga tacaggcggc gcagccgata cattggtatt gcagaccgtt gcggatgtcg
    14701 atgccgacgg ggcagacttg gacgcacatc gcacagttga tgcagtcgcc caaacccgcc
    14761 tcttccttat tgaccgtttt cttgcgcgcg ccgcgcggtt cgccgcgttc cgcgtcataa
    14821 gaaacaatca gcgtgtcctt gtcgaacatc gcgctttgga aacgtgcata cggacacata
    14881 tgcaggcata ctttttcacg cataatgtgg gcgaagaaga aggtcataaa gccgtaaaac
    14941 gctgcggcaa acatcgcgcc gccacctgct gctccagtga ataaatcggg aacgaactgg
    15001 cggataggga caaaccagcc tgcaaacgtg atgcccgtcc acgcgcagac aaggaaaatc
    15061 agcaggtatt tggtggcttt gatgcggatt ttagtgaaat tccacggcga tttttccagt
    15121 ttcagccgtt tgtttctatc gccttcgacc aggttgtcaa tccacagcat aatttcggtg
    15181 taaaccgttt gcgggcagga atagccgcac cacagtcgcc ctgcaatcgt cgtccaccaa
    15241 aacagcccga aggcgcaaat catcagcagc aaggcaaggt aaatcaaatc gcccaccccc
    15301 aacgacaatc cgaaaatgaa gaaatgccgt tcggggatat tgaaaacgac ggcctgcctg
    15361 ccgctccagt tgaaccacgg aatgacgtaa aacacaaact gcgtcgccaa tacggcggcg
    15421 atacgcagtt tggcgaaccg tccttccgcc tttttgggat ggatgcgttc gccttcggga
    15481 tggatttgaa tcacgctgac tcgcggatcg aatgtttttt ttgctttcgg cgcggctttt
    15541 gtttgttcgg acgtgccgat tccggatgcc ggactgccgg cttggttttc cgtggtcatt
    15601 ctgcattcct tagatttttg attgatggtt tgcccgttac cgccgccgtt tgcttttcag
    15661 acgtcatttt tcttgttttt taaggcgttg tgtttcaagt tttgagaaaa tccgtttttc
    15721 ccaaaatata tttccgctat tgtacaactt tatgcgccgt ccggatgtat ggggcggata
    15781 catttcccat ccgcatcaaa acgcctggat tttaccttac tgcccgaaca aaatccgaat
    15841 acggttaaaa aaaaagacta aaaaaaccga cacccccata tcggcagaac cgacggtgca
    15901 agctcataaa caaacgctat cgacaatccg gcacacaatc tataactttt tatttcaaaa
    15961 ggaataatgg caggcttcgc ccgcaaatcg aaaatccttc cccaccggtc tcctgctgcc
    16021 gccttccccc gcgtccgccc ttttcttgaa agcataagcg aatcgggcga taatcaacgc
    16081 tttccgatta tccacttatc cgaaacacca gcaaggaaaa tacaaaatgt ctcaactggc
    16141 aaacgcaatc cgcttcctct cggccgatgc cgttcaaaaa gccaattccg gccaccccgg
    16201 cgcgcctatg ggtatggcgg aaatggcgga aacattgtgg acgaaattcc tcaatcacaa
    16261 tcccgccaac cccaaattct acaaccgcga ccgcttcgtc ctctccaacg gccacgcgtc
    16321 tatgctgttg tacagcctgc tgcacctgac cggctacaat ctgagcattg aagacttgaa
    16381 aaacttccgc caactgcaca gcaaaacccc cggccatccc gaatacggct ataccgacgg
    16441 cgtggaaacc acgaccggcc cgttggggca agggattgcc aacgcggtgg gcatggcatt
    16501 agccgaaaaa atccttgccg ccgaatttaa taaagatggt ttgaacatcg tcgatcatta
    16561 cacctacgtc ttcatgggcg acggctgtct gatggaaggc gtatcgcacg aagcctgttc
    16621 gctcgccggc actttaggct tgggcaaact gattgttttg tatgacgata acaatatttc
    16681 cattgatggt aaagtggacg gctggtttac cgaaaacatc ccgcaacgct ttgaaagcta
    16741 cggctggcac gtcgttccta acgtaaacgg tcatgacacc gccgccattc aagccgccat
    16801 cgaagccgca cgtgccgaaa ccggcaaacc gtccatcatc tgctgcaaaa ccttaatcgg
    16861 caaaggcagt gccaacaaag aaggcagcca caaaacccac ggcgcacctt tgggcgcgga
    16921 cgaaatcgaa gccacgcgca aacatttggg ctggacttac cccgcctttg aaatcccgca
    16981 agaaatttac gatgcgtgga atgccaaaga acaaggcgcg aaactggaag ccgactggaa
    17041 cgaactgttc gcgcaatatc aagccaaata tcctgccgaa gccgcagaat ttgtgcgccg
    17101 tatggataaa aagctgccgg acaatttcga tgaatacgtt cagacggcat tgaaagaagt
    17161 gtgcgccaaa gccgaaacca tcgctacccg caaagccagc caaaacagca tcgaaatctt
    17221 ggcaaaagag ttgcctgaat tggtgggcgg ttcggccgac ctgaccccgt ccaacctgac
    17281 cgactggtca aacagcgtct ccgttacccg cgacaaaggc ggcaactaca tccactacgg
    17341 cgtgcgcgag ttcggtatgg gtgcgattat gaacggtctt gccctgcacg gcggcatcaa
    17401 accattcggt gcgaccttcc tgatgttcag cgaatacgag cgcaacgccc tgcgtatggc
    17461 ggcactgatg aaaatcaacc ctgtatttgt gttcacccac gattccatcg gtttgggcga
    17521 agacggtccg acccaccaac ccatcgagca aaccgccacc ctgcgcctga ttccgaatat
    17581 ggacgtatgg cgtccgtgcg acaccgccga atctctggtg gcttgggcag aagccgtcaa
    17641 agccgccgat catccgtcct gcctgatttt cagccgtcaa aacctgaaat tccaagcgcg
    17701 cagcgaacaa caactgaacg acatcaaacg cggcggctac gtcatcagcg aagcccaagg
    17761 caacgcccaa gccgtcatca ttgccaccgg ctcagaagtc gagctggctt tggaagcgca
    17821 aaaagcactc gccgcgcaaa acatcgccgt acgcgtcgtt tccatgccgt ccaccaacgt
    17881 attcgaccgc caagacgccg cctatcaagc cgccgtcctg cccgaaagcc tgccgcgcat
    17941 cgccgtagaa gccggccacg ccgacggctg gtacaaatat gtcggactga acggcgcagt
    18001 cgtcggcatc aaccgcttcg gcgaatccgc ccctgccgat ttactcttca aagcattcgg
    18061 ctttaccgtg gacaatgtgg ttgatacggt gaaatccgtg ctgtaacccc acacctaaac
    18121 aaatgccgtc tgaacgcttt tcagacggca tttttttgcc ctcccgcctt gatgcttcct
    18181 cattccgcca atctccatta taatatttgc gaatcactct tattcacatt tcaaaaggag
    18241 aaacgcatga gcacccgtac cgaacatgac accatgggca atgtcgaagt cccgtccgaa
    18301 gcctattggg gcgcgcagac ccagcgcagc cgcaacaatt tcaaaatcgg cggcgaaacc
    18361 ctgccgcagc cgctgattta tgctttggcg ctggtgaaaa aagccgccgc cgcgaccaat
    18421 gtttccctcg gcaggattaa gccggaacag gcggatttga ttacgcaggc ggcggatgat
    18481 gtgttgaacg gcaggcttga cggacagttt cctttagtgg tttggcagac cggctccggc
    18541 acgcagtcca atatgaacat gaacgaagtg ctggcaaacc gcgccaacga aatcgccggt
    18601 acgggtttgg cggcttatca gcccgtccat cccaacgacc atgtgaacca cgcgcaatcg
    18661 accaacgacg cattcccgac cgctatccac gttgccgccg cgattgaaat caaccgccac
    18721 ctcatccccg ccgtaaaagc cctgcgcgac acgttggaca aaaaagccca agctttcgcc
    18781 cctatcgtca aaatcggccg cacccacttg caagacgcga cgccgctgac tttgggacag
    18841 gaattttccg gctacgtttc ccagcttgat cacggtttag gccgtctgaa cgatgcgctt
    18901 aaagacttgt atgaacttgc tttgggcggt acggcggtcg gcacgggttt gaacagccat
    18961 cccgaatacg ccgaaaaagc tgctgctaaa ctcgccgaac tgtctggctt gccgtttgtc
    19021 agcgcgccga acaaatttga agccttaggc ggacgcgatg ccgccgttgc cgcttcgggc
    19081 gcattgaaaa cgctggcggc aagcctgaat aaaatcgcca acgacatccg ctggctggca
    19141 agcggcccgc gttgcggttt gggcgaaatc aaaatccccg aaaacgagcc gggttcgtcc
    19201 atcatgccgg gcaaagtcaa cccgacccaa tgcgaagcaa tgacgatggt gtgctgccaa
    19261 gtgttcggca acgacgttac catcggtatg gcgggcgcgt cgggcaattt cgagctgaac
    19321 gtctatatgc ccgttatcgc ctacaacctc ttgcaatcca tccgcctctt gggcgacgcg
    19381 tgcaacagct tcaacgaaca ctgcgccgtc ggcatcgaac ccgtaccgga aaaaatcgac
    19441 tatttcctgc accattccct gatgctcgtt accgcgttga accgcaaaat cggttacgaa
    19501 aacgccgcca aagtcgccaa aaccgcctac aaaaacaaca aatcgttgcg cgaaaccgcc
    19561 gttgagctgg gcttgctgac gggcgaagaa tttgacgaac tggtcgttcc tgccgatatg
    19621 gttcatccgc gctaatcttt ccctcaaata aaatgccgtc tgaaacctcg ttcagacggc
    19681 attttccgtt gcctgcgaac tagcggcgtt tgaacagcct gtcccccacc gccgccgtaa
    19741 ccgcaccccc gaccacgacc agtgcgccaa cataacccaa accgttcata tccggcgcgg
    19801 caaaagtatc aggcatcaca taatgcccga gcaaagaaaa tattacggta aacacgggga
    19861 gcaaggttgt taccgcgctg actttggaag cctcccaatg tttcaacgcc tcgccgaacg
    19921 agccgtaacc gattaacgta ttcaagcagc aatacgcaaa acaaacccac gccaacgtac
    19981 cgtccaaact tccgatgtgt gccggttcgg caaacggcag gaacacggcg gcacttgccg
    20041 cataaatcaa cagcagaatc tgttgcggcc cgaattgcgc cgacagcagc ttttgcgcca
    20101 cggcataaca cacccatgcc atactgcctg ccgcacacag caacacgccc ttcgcatacg
    20161 cgcccaaacc cgacaactcg ccgaatttat cgttaaaaaa cataagcaaa ccggcaagca
    20221 gcaaaaccaa gccgattttc tgagcggcag tcatccggtc tttaaacacc aacacaccga
    20281 caacaatcat cgtaaacggc gaaatctgcc acaaaacctg cgtcgtggtc ggcgaaatat
    20341 aatgcagccc ttgggcaatc agcacaaagt ttgccgaaat gcccgccacg ccgagcagca
    20401 gcagcctgaa tgagcaccaa gaaaaatccc gccgcttcgg cagccgcccg cccagtgcca
    20461 gcaaaacaaa caataccgcc gccgccacgg taaaacgcac ccacaccagc gtcggcgcat
    20521 cgacaaactt caatacctgc cgcacggcaa tcggcagcgt tccccacgtc atcgccgcca
    20581 aaagtgccaa cgcgaagcct aggagcggcc tttggttttc catcctgatt ttcctatttt
    20641 taaacaaccg tattgccgga cgatgccggt ttgccgcatc gggcaataat ggttcaagcg
    20701 tttggcgttt aattccaacc ctttgatttc aaacaaaccg gctgaagctc ggctattgct
    20761 tcgcgctatt tgaaaacacc gcctgaattt taaaatatag tggattaaca aaaaccagta
    20821 cagcgttgc
//
LOCUS       DAXHTR010000015        20696 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.015, whole genome shotgun sequence.
ACCESSION   DAXHTR010000015 DAXHTR010000000
VERSION     DAXHTR010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20696
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.015"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(61..237)
                     /locus_tag="ACFNML_000405"
     CDS             complement(61..237)
                     /locus_tag="ACFNML_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391846.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMRTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(240..1598)
                     /gene="murF"
                     /locus_tag="ACFNML_000406"
     CDS             complement(240..1598)
                     /gene="murF"
                     /locus_tag="ACFNML_000406"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFC6391847.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRAGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLTALQMMAKAWRENVDPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDDAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLKGFSNIKGRLNVKSGIKGATLIDD
                     TYNANPDSMKAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1723..3369)
                     /locus_tag="ACFNML_000407"
     CDS             complement(1723..3369)
                     /locus_tag="ACFNML_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6391848.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFIFGLTVWKYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYVAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3392..4870)
                     /locus_tag="ACFNML_000408"
     CDS             complement(3392..4870)
                     /locus_tag="ACFNML_000408"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFC6391849.1"
                     /translation="MFSKLTPLAETGIPTLSCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGILAAQVYGN
                     VSDGLKFWGVTGTNGKTSITQWLAQAADLLGEKTAIVGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATVAAMEVSSHGLDQSRVNGVSFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLKHAVINVDDEYGAELVGRLKKDCPDLAVYSYGFSEHADIRIIDF
                     TASSDGIAAVFQTPWGEGKCRTRLLGRFNAQNLAACIALLCANGYPLDNVLDVLAKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4895..6640)
                     /locus_tag="ACFNML_000409"
     CDS             complement(4895..6640)
                     /locus_tag="ACFNML_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HFC6391850.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPVDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPSPVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFGAEEMYDFYHELGIGVRM
                     HSGFPGETAGLLRNWRRWRPIEQATMSFGYGLQLSLLQLARAYTALTHDGVLLPVSFE
                     KQAVAPQGKRIFKESTAREVRNLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKFVNGR
                     YADNKHIATFIGFAPAKNPRVIVAVTIDEPTAHGYYGGVVAGPPFKKIMGGSLNILGI
                     SPTKPLTAAAVKTPS"
     gene            complement(6701..6964)
                     /gene="ftsL"
                     /locus_tag="ACFNML_000410"
     CDS             complement(6701..6964)
                     /gene="ftsL"
                     /locus_tag="ACFNML_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFC6391851.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6961..7947)
                     /gene="rsmH"
                     /locus_tag="ACFNML_000411"
     CDS             complement(6961..7947)
                     /gene="rsmH"
                     /locus_tag="ACFNML_000411"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFC6391852.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTTRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAVRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANPRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(7944..8399)
                     /gene="mraZ"
                     /locus_tag="ACFNML_000412"
     CDS             complement(7944..8399)
                     /gene="mraZ"
                     /locus_tag="ACFNML_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFC6391853.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLGQTDLQL"
     gene            8705..9895
                     /locus_tag="ACFNML_000413"
     CDS             8705..9895
                     /locus_tag="ACFNML_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6391854.1"
                     /translation="MSGNPINLHKLTLIMPLPLPSPEARQFSLKLQTLIAEKIGKHGN
                     WIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLARQLQELLSQT
                     AGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQARAPEASQKVV
                     HLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTYSARPLHDLQ
                     LSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDAAQYYHPQRN
                     QGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYLPQSHFLLNL
                     GITELLAQTGKTDSAAYIREAAAVQKLIDQHEMGELFKVIAFGKNIGVDWAGFRFGDI
                     CHKL"
     gene            10027..10103
                     /locus_tag="ACFNML_000414"
     tRNA            10027..10103
                     /locus_tag="ACFNML_000414"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10061..10063,aa:Met,seq:cat)
     gene            10111..10186
                     /locus_tag="ACFNML_000415"
     tRNA            10111..10186
                     /locus_tag="ACFNML_000415"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10144..10146,aa:Ala,seq:ggc)
     gene            complement(10468..11289)
                     /locus_tag="ACFNML_000416"
     CDS             complement(10468..11289)
                     /locus_tag="ACFNML_000416"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFC6391855.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGLGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEYLFNPLSVAVMLVLGGFFILWVEKRQSRAEPKIADVDALRPIDALMIGVAQVFA
                     LVPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFIAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11322..11966)
                     /locus_tag="ACFNML_000417"
     CDS             complement(11322..11966)
                     /locus_tag="ACFNML_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6391856.1"
                     /translation="MKLKHLLPLLLSAALSAQAYALTEGEDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKIRLENRAVAGKWALSQKGFDGKKLMRAYDSPEAAAAALKMQK
                     LTEQYRIDSTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQTPAVQK"
     gene            complement(11969..12832)
                     /gene="ftsN"
                     /locus_tag="ACFNML_000418"
     CDS             complement(11969..12832)
                     /gene="ftsN"
                     /locus_tag="ACFNML_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6391857.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPV
                     PSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12938..14434)
                     /locus_tag="ACFNML_000419"
     CDS             complement(12938..14434)
                     /locus_tag="ACFNML_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFC6391858.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSHRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGESSASVLERVIAARDKQYARQGKVNAALSVSELDSQARIQKEAQEAL
                     GGLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14448..14768)
                     /locus_tag="ACFNML_000420"
     CDS             complement(14448..14768)
                     /locus_tag="ACFNML_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HFC6391859.1"
                     /translation="MFGKQLFEEVGSKISETIVNSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15252..16778
                     /gene="putP"
                     /locus_tag="ACFNML_000421"
     CDS             15252..16778
                     /gene="putP"
                     /locus_tag="ACFNML_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFC6391860.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIVYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            17029..20634
                     /gene="putA"
                     /locus_tag="ACFNML_000422"
     CDS             17029..20634
                     /gene="putA"
                     /locus_tag="ACFNML_000422"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HFC6391861.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4840 a         5986 c         5058 g         4812 t
ORIGIN      
        1 ggcctgcgcc cgtttgaggt tggcttgccg cccggcaagg tttcggacgg cttttgcaga
       61 ttaacgtgca gggactttct caatcacgca aattgggtaa ctccccggat ttttaccgcc
      121 gcccaagtct aaacataaat cttcataaag atacgcctgc gtccgcattc cggcataaaa
      181 cgcgccgacc agcgcggcaa tcagcaataa ggctttaaaa aaacgtcggc ttttcatttt
      241 cacttatcct ccaatgcctc gaccacttct tccatctgca taaagcgcga acctttcacc
      301 aacacggtgg cgcgttcggg caaatcgtgg cgcaacactt gaatcaacgg gtctttggcg
      361 gcgaaccaca aaccgtccgc gccaaatttt tccgccgctt cgacgctgtt gtcgccgaca
      421 aaataagccg cttcgatgcc ttggtcgcgg gcatacgcgc cgacttcggc gtgcatagcg
      481 gcggcttcgt cctcgcccag ttcgcccata tcgcccatta cgaaaatacg cggcgcaggc
      541 atacgcgcca acacgtcaat cgcggctttc atgctgtccg ggttcgcatt ataagtatcg
      601 tcaatcaggg ttgcgccctt gattccggat ttgacgttca gacggccttt gatattgctg
      661 aagcctttca aaccttccgc cacatcgttc aaactcaaac ctgcagccaa agacagcgcg
      721 gcggcagcgg cggcgttgtg gacattgtgg cggccgggaa caggcagcac cacggcggcg
      781 cgttcgtcgc cgcacaccaa atcaaattcg cacgacaacg gtttcagcac gatattttcc
      841 gcgtggacat cgccgctatc gatgccgaaa gtgcgcgtat tcaaattaag cgttgccgtt
      901 ttgaagacag ccatattggc atcttcttga ggaatcagtg ccatgccgtc tgaacataag
      961 ccttgataaa tctcgctttt cgctttggca atatcgccca ctccgtcgaa accgcagccg
     1021 acatgggcgc gcatggcgtt gttgaccaat gcggcatcgg gtttggcgat ttgtgtcaaa
     1081 accgccagtt cgccaaaatg gttcataccc atttcaatca cggcatagcg gtgtttttcg
     1141 tttaatttca acaaagtcaa cggcaatccg atgtggttgt tgaagttgcc tgccgtcgcc
     1201 aaaacggcat catcaccgaa acggcggcgc aataccgcag ccagcatttc cttcaccgtc
     1261 gtcttgccgc ccgaaccggt aatgccgaac acaaacggat ccacgttttc acgccacgcc
     1321 ttcgccatca tttgcaacgc ggtaagcgtg tcatcgactt tcaacgcgcc atccattgca
     1381 acgcaatctt cgcgcgaaac cacaaccgcc gccgcaccag cagccaatac gtcttcaaca
     1441 aaatcatgcg catcaaaccg cccgcccgcc aatgcgaaaa acacatcgcc cgcgcggatg
     1501 tcgcggctgt cggttacgat gcgcgacacg ggtttgcttt cagacggcat cggaagcttg
     1561 agggcttggc agatgaaatt taggtccagt ggtttcatat ttactttcgt taatattcgg
     1621 gcggcggaca catcggtagc ggctgatttt tttatcgcct gttttgctgt ggtaaaacac
     1681 agattatttt cccattctca ttcggcattt tttctgtacg tatcattttt tagacgtatt
     1741 tttagtcgat ttgccttttc ccgcatacca cggcgcgggg tcgtcgggca gtccgtcgat
     1801 aaaggcaagg ttattgcctt cgccctgcac atcgggaaca ttcccccaaa aatcatagcc
     1861 gtcatcgggc aactcgtcgg tttcgatacc cgtccaactg ccgaatccgc gtaaaaaatt
     1921 aaacgcgctg cgcctcactt taatcatttc gcgccgcgtg tcatcggacg aaattttaac
     1981 cagcggcacg ccgtagcttt gacgcttcca cgcaccatgt gtcaacgttc gctcgccgcc
     2041 tttaccgaca tgcatcaaac cgtggtcgga aaagtaaacc aaagaccagc tttctttatt
     2101 ttcattcaat atcttaactg tatcttctaa aaatttatcg gtttgcgcga tggtggaaac
     2161 atagcaggat attttttcag tttgatactg aaaccgccgc gcatccttat ccaaacgtgt
     2221 gcaaaaatca ctgtgcgaac ccatcaggtg catcacaatc agccgaggct tcgtgcctgc
     2281 atttcctatc aaaacccgtt tgaacgccgg caacaaaagg cggtcgctca accccgcgct
     2341 tttgccataa tcaccccttt gggtaaacca cggataatcg ctgcgtaggg cataggtgga
     2401 aatttcgttg gcaaaatgcc ccaacattcc ttgattagac agccacgccg tccgaaaacc
     2461 cgcctgcttc gccaagctga cgatgttatt gttcggttct cccggcagcc ccaaagtctg
     2521 cggcagcgaa agattcgtcg cgtgggcggt cgattggtaa ccgtttatca acagcccttt
     2581 ggtctgactc aaaaaaggcg tatcgggcaa tgggaaaccg taaacattca tataatccga
     2641 acgcgcgctc tcaccgataa ccacaacata atttttatac ttgggcgcaa catgacggat
     2701 atgccatgtt gacgcttttt ttgctgcttc caaaatgtgg gcgcgcttgg cgacatattg
     2761 tgccggcgcg gaagccaaat cgtaatacag gtcgaaaata ttcaacaaca ggccggcatc
     2821 aggttctcgc aaatctttat cgctggcgat tttatccatc accgcgcagg acaaaatcaa
     2881 agtcaataat atagtcagcc atattttgct gcggcgttta tagtttttta cgtcagcaaa
     2941 tacccccccc gatacacaat atttccaaac tgtcaagcca aaaatgaaaa ataatgcctg
     3001 cacaaaataa agcgacccgg gaagattgcc gacaaattca cgcgcctcgg caggattgct
     3061 ttccaatatc gaaccgacta tctgataaga cggcgcacca tacaaccagc cgaccggcaa
     3121 atatagggcg gttgtgccga cgtaaatcag taaaacaact gaagaaacgc gcgggaaacc
     3181 gcgtgccaat aataataaaa atacaaccga agccaaagca cccacggcag aataacggta
     3241 gccgtattca tattccaagt gataacctga tgcgatggcc gcgccaaaac aaaaggctaa
     3301 aagcgcgcaa atgcccaatg tattttttaa tgtctgatcc atatttttta ttcgggcgga
     3361 ttgtttcata cgatgccgtc cgaatatccc attatttacg agttaacaaa gcctgtccga
     3421 cgatttcaag atcggaaaaa cggtgcttca cgccttgtac atcctgatag ttttcatgcc
     3481 ctttgccggc aatcaggatg atgtcgtttg cggcggcttg ttcaaccgca taacggatgg
     3541 cggcggcacg gtcggcttcg acgcattcgg gcgcgggaac ggcaggcagg atgtcgttga
     3601 tgatgtcgtg cggattttcc aaacgcggat tgtcgctggt gacgacgact ttatccgcgc
     3661 cctgtacggc tgccgcgccc atcagcgggc gtttgccgcg atcgcggttg ccgccgcaac
     3721 cgaatacgca ccacaaagcc gcaccctgcg gtttgatttc ctgcaaggtg gcgagtgctt
     3781 tttccaatgc gtcgggcgta tgggcataat cgacaacgac caaaggtttg ccgctgttca
     3841 tgatgcagtc catgcgccct gaagcgggac ggatttttgc cagcacatcc aatacattat
     3901 caagcggata gccgttggcg cacagcaagg cgatgcaggc ggcgaggttt tgcgcgttga
     3961 accgtccgag caggcgcgtg cggcatttcc cttcgcccca cggggtttgg aatacagctg
     4021 ctatgccgtc tgaagaggcg gtgaaatcga taatgcggat gtcggcgtgt tcgctgaagc
     4081 cgtagctata aacggccaaa tcgggacagt cttttttcag acgacctacg agttccgcgc
     4141 cgtattcgtc atccacgttg atgactgcgt gtttcaagcc gtgccagtaa aacaggcgcg
     4201 acttgatggc accgtaggct tccatcgtgc cgtggtagtc gaggtggtcg cgggtgaggt
     4261 tggtaaagat tgcgctgcgg aatgacacgc cgttgacgcg cgactggtca agcccgtggc
     4321 tggagacttc catcgcggcg actgttgcgc cttgttgacg gaaacggtag agcagggttt
     4381 ggacatcgac gggggcgggt gtggtatgcg tggtttcttc caatgcaccc caaaagccgt
     4441 tgccgaccgt gccgacaatg gcggtttttt cgcccaacaa atcggcagct tgcgccagcc
     4501 attgcgtgat ggaggttttg ccgttggttc cggttacgcc ccagaatttg agaccgtctg
     4561 aaacgttgcc gtaaacttgc gccgccaata tgccggcacg gtgtttcaaa tctttgatgc
     4621 cttgattggg gactttccat tcgggattcc acgcaaattt gccgtcgtcg tcccaaaaaa
     4681 caaaagccgc gccgttggca acggcggcgg ggatataact gcgtccgtcg gcatattcgc
     4741 ccggacaggc aacgaaaata tcgccttgtt tgatttggcg gctgtctgaa tgcaacaaac
     4801 gccctgccgc gtttgcacac gacagggtcg ggatgccggt ttcagccaaa ggggttaact
     4861 tgctgaacat aaaacaatct cgttgatact cggattaaga cggtgttttg acggctgcgg
     4921 cggtcagggg cttggtcggg gaaatgccca agatgttcag gctgccgccc ataatttttt
     4981 tgaagggcgg ccctgccact acgccgccgt aataaccgtg ggcagtcggt tcgtcaatgg
     5041 ttaccgccac aatcacacgg gggtttttgg cgggggcaaa accgataaag gtagcgatgt
     5101 gtttgttgtc ggcataacgc ccgttgacga acttgcgcgc cgtgccggtt ttcgcgccga
     5161 catcgaaacc gtccaccgca cccgccgtac cggtgccgcc cggctcggtc acggaaacca
     5221 tcagattgcg tacctcgcgc gcggtcgatt ctttgaatat gcgtttgcct tgcggcgcaa
     5281 ccgcctgttt ttcaaagctg accggcagca aaacgccgtc gtgcgtcagt gcggtatagg
     5341 cgcgcgccaa ttgcagcagg ctcaattgca ggccgtaacc gaaagacatc gtcgcctgtt
     5401 caataggccg ccacctgcgc caatttctca acaaacctgc agtttcgccc ggaaagcccg
     5461 agtgcatacg cacaccgatg cccaactcat gatagaagtc atacatttct tcggcaccga
     5521 aacgcgcaga cagtttgctt gtgccgacgt tggacgattt ctgcatgatg ccgcgcacat
     5581 ccaaagaggg gtaaacatgg gtatcgcgca cgggagacgg tccgatttta taaggctgcg
     5641 tattcagccg ttcgttcaaa tcggttttgc ccgcatccaa tgctttcgca atcacaaacg
     5701 gtttgattgc cgaaccgggt tcgatcatat cggttacggc acggttgcgc cgctgttcgc
     5761 tgtctgcccg gccgggcctg ttgggatcgt aggcgggcgt attggccaag gcgaggattt
     5821 cccccgtgcg ggcatccaaa accaccaccg ttccggcttt tgcctgatgg tattcgaccg
     5881 ccttgttcaa ctcttcatag gccaaggtct gaatcctctg atcgagggaa aggatgatgt
     5941 ctttgccgtt tttcggggct ttattgcgcg gggagtccaa gctgtccaca atattgccct
     6001 gccggtcccg caaaacgact tccgcgccgt cttcgccatg caggctgtct tcaagcgaaa
     6061 gttccaaacc ttcctgacct ttgccgtcaa tatcggtaaa tccgatgacg tgtgcaaaca
     6121 ggttgcccat cgggtaatgg cgttttaatt ctttttcaaa tacaaagttt tccaaaccca
     6181 aggctttgac ctcttcggca accttgggat cgagctgccg cttaatccag ataaacgact
     6241 tgcctttctg ttcgagcttg ttcctcaaaa catcaaccgg cacatcgaca agctcggaca
     6301 ggcgttccaa ttgtgcggca gacggcattt ccttcatctc tttaggcacg gcaaacaggg
     6361 actccgtcgg cgcactcaac gccaaaaccg caccgttccg gtccgaaacc gtaccgcgtg
     6421 tagccggcaa tgtttgagtc cgcacaatcc ggttgtcgcc ctgttctttc aaaaagttat
     6481 acgttaccgt ctgcagatac agtccgcgag caatcagacc ggcaaacaag accgctattg
     6541 ccatcaggac gaagctgatc cgtccgttac tggtcatcgg ctttttgacc tgctcttctt
     6601 tgggcagcat ccgaggctta tattcgctct taatcaacat ttttacttct cgttattatt
     6661 atcctgacgc aggaatccga ttccggcaca caggctgctt ctatctttga tgcgccacca
     6721 taaaggtatt gcccgaaacc ggcggatgga ggttttgttt ttctgccgcc gccctgatcg
     6781 cttcgtggtt cgccaaacgc gcctgttgca gcctcatttg cgcataatcc tgctccaagg
     6841 cgatttcctg ttttttcgcc ttgtccaaag ctgtgaagtt gagcctgtac tggttttgct
     6901 gcatcacaac ggaaaaagcg gaaacgcaca ccgcaagcag cagaaggaaa ttcaatttgt
     6961 tcattgccat tcagacgttt ttctctgtga ttgttccggt atcgaaccgg cagtccgctc
     7021 cgccacacgc aaaaccgcac ttctcgccct cggattggcg gcaatttccg cctcacccgg
     7081 ctttaatgcc ctgcccacga ttttcagggg cggctcgggc aaatccgctt ccctgaccgc
     7141 cgcccagcgc ggcaggggcg cgtgttgcga atattttttg acaaactgct tcacaatgcg
     7201 gtcttccaac gaatggaaag caatgaccgc caaacgtccg ccctctttca gacgacacat
     7261 gacctgcggc aatactgccc ctacttcttc aagctcgcgg ttaataaaga tgcggaccgc
     7321 ctggaaggtg cgcgtcgcag gatcctgccc ccgctcgcga gtacggacgt tttgtgccac
     7381 gatctgcgcc agcttgcggg ttgtatcgat tggactttcc gcccgttgcg caacaatggc
     7441 gcgcgcaatc tggcggctaa accgctcttc accataattc ttgattacct cgtgcaaatc
     7501 ctgttccgac gcaaccgcta tccactctgc ggcagacata ccgcgcgtcg tatccatacg
     7561 catatccaaa ggggcatcga aacggaagct gaaaccgcgg ctgccgtcat cgatttgcgg
     7621 ggacgaaatc cccaaatcaa acagcgcacc gtccaccttg ccgataccca aaccgtccaa
     7681 tgccgtctga aacgaagcaa aaccgccatg cacgacaccg acccgtttgt ccgaacgcgc
     7741 cagctcttct gccacagcaa tcgcctgagg gtctttgtcg aaaacaatca accgccccgc
     7801 atcgcccaaa cgcgacaaaa tcagccggga atgccctccc ctgccgaacg taccgtccac
     7861 atagacaccg tcttcgcgca cggcaagcgc atccaccgcc tcattcagca agaccgtgat
     7921 atgccggtaa ctttctgctc cactcacaat tgcaaatccg tctgacccaa ctggaaggca
     7981 agttcgtcag gatcgtcatc caaagcctga accatctcag cctcccactg ctcgcgaccc
     8041 caaagctcca aacggttggc acgaccgacc aaaacgactt cacggtcgaa atccaccctt
     8101 ttcctcagtc cggcaggaag cagcacccgg ccggcgctgt cccattccaa aatttccgcg
     8161 ttatgcagca aaagattttg aaaccgccgc aaaacagggt tatccgccac ttttaagttt
     8221 aaaagttgcg ccgcaacctt ttcccactcc gcaacagggt acatcaacag cttgtgtttc
     8281 gactcgagcg ttaccactac ggcaggcgta tagaggcgcg acagaatgtc acggaatttg
     8341 gcaggaacag ccaaccgccc cttactgtcg atgcttaatt cgtgtgcgcc gccgaacatg
     8401 acatgtccca agccgaaatc aaaatcacaa gggtaaaaga gacactttgc cccacaattc
     8461 cccaccaatc gacactataa gaaattttaa acactcggtc aaatcagggc atgaaaactc
     8521 attaacatat ctgaaatttt tattcctttt aaaaacaata agataaaaaa tgacgacaac
     8581 ggcacggcgg gtgcggtaca gaataatcga accaataaac aactatatat gattaattta
     8641 ataatataaa cacaatatat agtattaaga taaagccatg acagcacccg taccaacgtg
     8701 taatatgtcg ggaaatccaa taaatttaca caagctaaca cttatcatgc ccctccccct
     8761 cccctctccc gaagcacggc aattctcgct caaactgcaa accctcattg ccgaaaaaat
     8821 cggcaaacac ggcaactgga ttccattttc acgttttatg gaattggttt tatacgctcc
     8881 gcaatacggc tactacaccg gcggcagcca taaaatcggc aataccgggg attttattac
     8941 tgcaccgacc ctcacctctc tgtttgcaca gacactggca cgccaacttc aagaacttct
     9001 atcccaaacg gcgggcaata tctatgaatt cggcgcggga accggacagc ttgccgccga
     9061 tttgttgggc agcatttcgg acggcatcag ccgttactat attattgaaa tatcgccgga
     9121 gctggcagca cgtcagaaaa acctgattca agcccgcgca ccggaagcat ctcaaaaagt
     9181 tgtccacttg accgcacttc ccgaagcgtt tgacggcatc atcatcggca acgaagtact
     9241 cgatgccatg cctgtcgaaa tcgtccgtaa aaatgaaggc ggctcattcg agcatgtcgg
     9301 cgtttgccta gataatgacc gttttaccta ttcggcacga ccgctgcacg acttacagct
     9361 atctgccttg gcttccctct attttcctca aacagattat ccctacacca gcgaactaca
     9421 tccgcaacaa tatgccttta tccgcaccct tgcctcaaga cttgaacgcg ggggtatgat
     9481 attcatcgac tacggttttg atgcagcgca gtattaccac cctcaacgca atcaaggtac
     9541 tctgatcgga cactaccgac atcacgttat ccacaatcct tttgacttca tcgggttggc
     9601 cgacctgacc gcgcatgtca actttaccga cattgcccaa gccggtacgg atgccggatt
     9661 agatttgata ggttaccttc cccaatccca tttcttattg aacctgggca ttaccgagct
     9721 attggcacag acggggaaaa cggattcggc agcctatatc cgtgaagctg ctgccgttca
     9781 gaaactgatt gaccagcatg aaatgggcga actgtttaaa gtcatcgcat tcggaaaaaa
     9841 tatcggcgtc gactgggcag gattccgctt cggcgacatc tgccacaaac tctaaccctc
     9901 atgccgcctg aatccgcttc agacgacata aactttttaa catttaaaaa cagtcaacta
     9961 attcaaaatt aaaaaatacg gcttgtcaaa aaaacagaaa aacatataat agcgtcttca
    10021 cgaaacggcg aattagctca gtcggttaga gcagaggaat cataatcctt gtgtccgggg
    10081 ttcgagtccc tgattcgcca ccaaattttc gggggtatag ctcagttggt agagcgcttg
    10141 catggcatgc aagaggtcag cggttcgatt ccgcttacct ccaccagata aaaaagcaca
    10201 gaccgtaaaa aggtatgtgc ttttttattg cctgattgcc agcaaataaa gaataaacca
    10261 ctgccttcaa aacaggcaat cgactttaaa cctatcgccc cgcctgtcct gattttatag
    10321 tgaattaaat ttaaaccggt acagcgttgg ctcgccttgc cgtactatct gcggcttcgt
    10381 cgccttgtcc tgatttttgt taattcacta tatcagcccg ccagacaaac ccgacccgaa
    10441 taatgtcttc aggtcgggtt tatggtttca ttcccaactt atccagcctg acagccacaa
    10501 tataatgatg gcaataccaa aaacaatgcg gtaataggca aaaggaatat aatttttctt
    10561 ggaaacaaac ctcagcaacg cttttaccgc taccaagcct gaaacaaagg cagcaataaa
    10621 gcctatcaga atcaaaccga catcatgcag ggtgaaaaat cggtaatgtt tcaggacatc
    10681 ataagccgtt gcggcaacca tcatcggcac agccaagaaa aacgagaatt ctgtcgcagt
    10741 tttccgttcg atgccccaaa gcatcccgcc cataatcgta ctgcccgaac gggacgtacc
    10801 cggaaccagt gcaaacactt gggcaacgcc gatcatcaag gcatcaatcg gacgcaatgc
    10861 atcaacatcg gcaattttag gctctgctcg gctttggcgt ttctccaccc acaaaataaa
    10921 aaaaccgccc aaaaccagca tgactgcaac actcaagggg ttaaacagat actctttgat
    10981 ttgtttgccg aacaacagcc ccatcacggc ggcaggtata aaagcaatgg caagattaag
    11041 gacgaagcgg ttggctttcc ggtcttttcc caagccgtgc aacacattgc tgaaacgttg
    11101 ccggtattca aacactaccg ccaaaaccgc accgagctgg atggcaattt caaaaacctt
    11161 gtgattgctg tgaaaaccaa tcagattgcc gaacacaatc aaatgtccgg tgctggaaat
    11221 cggtaaaaat tcggtaaaac cttctaccaa gcccatcatc agggctttca ggacaatcag
    11281 aaaatccatt gcttgcgctt ctttcggata cgggagttcg gctatttctg tacagcaggg
    11341 gtctgacgct tgcgttcttc cctgactttg gcaaccaatt ctttaatcgt atgaacgccg
    11401 ccgtcaaagc cgttattgaa gataacgcgg tattttccgc cgacaataac ggtcggcgtg
    11461 ctgtcgatgc ggtattgttc cgtcagtttc tgcattttta atgcggcggc ggcagcttcg
    11521 ggggaatcat aggcgcgcat cagttttttg ccgtcaaagc ctttttgaga caaagcccat
    11581 tttccggcaa cagccctgtt ttccaagcgg attttttgtt cgtaaactgc tttaaacacg
    11641 gcagggtttg cctgatattt caaacccgac aaattgacgg cagccgccat cctggccaga
    11701 ccgagcattt caggctgcca gaccacgtgc tccgtcctca aataggcatc agacggcaat
    11761 gccttgccca gtttcaataa caaaggatcg aaatgatggc aatgtacgca gaaatagccg
    11821 aaaaattcca aaacctcaat tttacccgac tgttcttgag gaatgggttt atccaacaca
    11881 agatagtctt ccccttccgt cagggcatat gcctgcgcgg acaacgctgc cgacagcagc
    11941 agcggcaaca gatgtttgag cttcataatt atttactttc gatagaacgg atcaggctgg
    12001 cgacttcatg ttttttcaac tcgtcctgca tttttttcac cgcatcggca gacatattgc
    12061 cgctttgcac ccggtaaagc gttttatgtc ccgcctgata accgaccacc ttggaagata
    12121 tgcccaagat tgccagtttg gcacgctgtc cttccgcgct ccggcggtcg gcatacgcgc
    12181 ccatttgcag ataatgcgtt gcttccgcct tgtcgggcgt tttcattttc tgcacttctt
    12241 tggcagcggc actgcgcgct ttttcgatgc tgccgctgtt gaggatttgt tccggggtcg
    12301 gtttgggtgc aactttttcc ttctccgcct ttttctcttc ttttgaagct tttttctctg
    12361 tttctttaga tggttttacc gcttgttttt taaccgtttc ggcatctttc ttctgcgctt
    12421 tttccccgac ggtttgttca cgctcttccg tcagtgcttt cttgcgcact gcctgtccgt
    12481 ccgacttttc ccgctccggc tcgtccgcct tttcctcaac ctcgtcggct ttgtcggcaa
    12541 cgggctgctt gtcggcagct ttttccgcat ccgactgctc tgcctctttc gcagcatccg
    12601 gttcggacaa ggcgttttga tcggccggtt caggttggat gtcttcctta ggctggtttt
    12661 tcggtttcag gatttccgtt tctgcaggct gcttcgacgg aaccgggatt ttgaacgcat
    12721 tttgaccgct ctggttcaga taaaacaaaa taccggcaat aatgaccgtc gccagtatca
    12781 aaccgaagaa aaaaccggac agaccttttc cggattggga aaatttgttc ataaacatac
    12841 cttaatgtgt ttcagacggc attaacgccg tttgcttacg ggcggatatt ctaacaatat
    12901 cgccatattt gggcaaaacc tgcttccatt cccattccta taaagcacga cggaaaccta
    12961 tggctttcat gacgtggctt ctgccgactt cttcgtcgcc cgccaaatcc gccaatgtac
    13021 gcgccacgcg cataatgcgg tggaagctgc gggcggaaag ggagagtttt tccagcaggc
    13081 cgcccaatgc ttcctgcgct tctttttgaa tgcgggcttg ggaatcgagt tcgctgacac
    13141 tcaaagcggc gttgactttg ccctgccgcg cgtattgttt gtcgcgggcg gcgataacgc
    13201 gttccaaaac ggacgcgctg ctttcacccg cttcctgctg catcagttcg gcggcggaca
    13261 ggctggggac ttcgatggtc aaatcgatgc ggtcgagcag cgggccggaa attttgctgc
    13321 ggtaacgcgc gacgctttcg ggcgtgcagc ggcagggttt gacgggatgc ccgagataac
    13381 cgcacgggca ggggttcatg gcggcaacaa gttggaattt ggcaggatag acggcttggc
    13441 gcgccgcgcg ggaaatgtgg atttcgccgt tttccaacgg ttcgcgcaaa acttccaaaa
    13501 ctttgcggtc gaactcgggc agctcgtcca aaaacaatac gccgtggtgc gccaatgaaa
    13561 tctcgcccgg acgcgggtcg gaaccgccgc cgaccatagc cgccgcgctg gcactgtgat
    13621 gcggactgcg gaaaggacgg tggctgtcga gttgttgctg gtggttgggc aggagcgaac
    13681 gcaatgccca aacttcaacc aattcgtctt cggtcagcgg cggcaggatg cctggcagcc
    13741 gttgggagag catagacttg cccgttcccg gcggacccat catcaagagg ctgtgtccgc
    13801 ctgcggcagc gatttccaaa gcaaggcggg cagtgtgctg acctttcaca tcacacaaat
    13861 caggttgtcc gccatgttca gacggcattt gcggtacgga acattcggtt tgcgccaaag
    13921 gttcgatacc gttcaaatgg gcggcgactt cgcccaaaga gcgcgcgccg taaacggtaa
    13981 tgccgcgcat cacggcggct tgtcctgcgt tttcttcagg caaaacaaat gcacgttttg
    14041 cctgcatacc ctgccacgcc atcgccaacg cgccgcgcac ggggcgcaac agccccgaca
    14101 gtgccaattc ccccgcaaac tcgtattccg ccagtttttc gggcgcaacc tgccccgatg
    14161 cggcaaggat gccgattgca atcggcaaat cgaaacgccc cgactctttg ggcaggtcgg
    14221 cgggggcgag gttgacggtg atttttttgg cggggaattc aaaaccgctt tgaataatgg
    14281 cggcacggac gcggtcgcga ctttctttga cttccgtatc gggcagtccg acgatgttga
    14341 aatgtggcag gccgttggca aggtgggctt ccacttcgac caacggcgca ttcataccgc
    14401 tcaaggcgcg gctgtaaacc aaggcaagcg acatatttca gacggcctta ttcgccggct
    14461 tcggtttgct gcctgatttc ggcgacggct tcttcggcag cggcttcagc cgcttccaat
    14521 gctgcccgtt cgggattttg cgcggcttcg agttttgcca aacgcgcttc caaagccgcc
    14581 agtttggtac gggttttgat taaaacctgc tgctggatgt cgaattcttc gcgcgtaacc
    14641 aaatccatac ggttgaacgc gccgcccagc atcgccttaa tatttttttc cacatctttg
    14701 gcagggctgt tgacgatggt ttcgctgatt ttcgaaccga cttcttcaaa aagctgcttg
    14761 ccgaacataa tctgtatcct tcctgaacat atcaaattca atcggctatt gtataaggaa
    14821 aaatgccgtc tgaaaacggg cggcggataa tcggcaaaac ataccgcgcc tcctttgcgg
    14881 ttgctaaatt acacctcaaa acacctgccg cctgaaaacg gatttcatat tccgcgcaac
    14941 gccagattac aagacactac aaaacaatat actgttttaa atgatttttc cgacgcgcat
    15001 cgtttcaaac tcggcttttt aagccattaa gtgctttgca aacaacagaa attgggttat
    15061 gctgaaacgg attatttaca atttcatata gttttattac atatcttatt gtgattgaag
    15121 ataatttatc cgaatccccc tttcgggtat ccggattttc cgttgtactt ttatttagaa
    15181 aaacatttca ggcgcaagtt gcttgcaatt tcaaagcctg taattctgaa gttttagacg
    15241 aggaatagca catgaatccc atgtacatta cttttgcaat ctatctggtt gcagtccttc
    15301 tcatcgggct tgccgcctat ttttctacgc gcaatttcga cgattatatt ttgggcgggc
    15361 gcagcctagg cccgtttgtt accgcgatgt cggcaggcgc gtccgatatg tccggctggc
    15421 tgctgatggg tctgccgggc gcgatttacc tgagcggttt gaatgaggct tggattgcca
    15481 tcggcctctt ggtcggcgcg tatttcaact ggctcttggt ggcgggccgc ctgcgcgtgc
    15541 ataccgaata cgccaacaat gcgctgacgc tgccggatta tttcttccac cgcttcggcg
    15601 cgggcggaca cttgatgaaa gtggtttccg cactaattat cttgtttttc ttcacaattt
    15661 actgtgcttc cggcattgtg gccggcgcaa ccctgttcca aagtctgttt gaaggtatga
    15721 cttacaacca ggcaatgtgg ctgggtgcgg gcgcgaccat cgtctatacc ttcttgggcg
    15781 gtttcttggc agtcagctgg acggatacgc tgcaagcatc tttgatgatt ttcgccctga
    15841 ttttaacgcc ggtgatggtc tatctgggct tgggcggcgc ggaacagatg tctgccgcga
    15901 ttcaaagcgt cgccgcaggc acgggcaaag aatacggcag cctgtttgcc ggtacgaccg
    15961 tcatcggcat catttccacc gccgcatggg gcttgggcta tttcggacag ccgcacattt
    16021 tggcgcgctt tatggcggcc gaaagcgcga aatcgctggt atccgcacgc cgcatcggca
    16081 tgacttggat ggcgttgtgc cttgcgggcg cggtagcggt cggttatttc ggcattgcgt
    16141 atttcggtgc aaatcccgac aaagtttctt ctatgagcgg caaccacgaa cgcatcttca
    16201 tcgcgctttc caccctgctg ttcaaccctt ggatcgccgg cattattttg agcgcgattt
    16261 tggcggcggt gatgtccacc ctgtcctgcc agcttttggt ttgctccagc gcgattaccg
    16321 aagacttcta caaaggcttt ttgcgtaaaa acgcgcaaca gtcggaactg gtatgggtcg
    16381 gccgcctgat ggtcttggcg attgccgtga tttccatcct gattgcttcc gatccgaaca
    16441 gcaaagtatt gggactggtg tcttacgcgt gggcaggttt cggtgcggca ttcggcccga
    16501 ttgtgattct gtccgtattg tggaaacgca tcactgccta cggcgcgttg tcgggtatgg
    16561 ttgcaggtgc cttaaccgtg gtcgtatggg cggaatgggt gaaaaaaccg gctcaagcgg
    16621 caggcgaaag cggcttgttg acgatgtacg aaatcgtccc gggctttatt gtttgcttga
    16681 tcgttgccgt gttggtttcc ctgttcaaca aagaaccttc gcgcgaaacc caagaacgct
    16741 ttgaaaaagc cgacgcagat taccgcgccg cccgataaac ccctttcaga cggcacggca
    16801 tcccggttcg tgccgtctga acccgtttcc gggtcaaaca caaataaacc ttgcctgcat
    16861 acaggcctta tttgtgtttt acccgtttca ttttcaaaga aacggaagca cctaccctgc
    16921 ccgcatttga agccttgtat ttcatctgcg gatttattac gatattaccg tattcagacc
    16981 gcaccgatgc cgtctgaacc ccccgaaaaa ctttggagaa tccaaaaaat gtttcatttt
    17041 gcatttccgg cacaaactgc cctgcgccaa gcgataaccg atgcctaccg ccgtaatgaa
    17101 atcgaagccg tacaggatat gttgcaacgt gcacagatga gcgacgaaga gcgcaacgcc
    17161 gcctccgagc ttgcccgccg tttggttacc caagtccgcg ccggccgcac caaagccggc
    17221 ggcgtggatg cgctgatgca cgagttttca ctctccagcg aagaaggcat cgcgctgatg
    17281 tgtctggcag aagccctgct gcgtatcccc gacaacgcca cgcgcgaccg cctgattgcc
    17341 gacaagattt cagacggcaa ctggaaaagc catttgaaca acagcccttc cctcttcgtc
    17401 aacgccgccg cctggggcct gctgattacc ggcaaactga ccgccacaaa cgacagacaa
    17461 atgagttccg cactcagccg cctgatcagc aaaggcggcg caccgctcat ccgccaaggc
    17521 gtaaattacg ccatgcgcct gttgggcaaa cagttcgtaa ccggacagac cattgaagaa
    17581 gccctgcaaa acggcaaaga acgcgaaaaa atgggctacc gcttctcctt cgatatgttg
    17641 ggcgaagccg cctacaccca agccgatgcc gaccgctact accgcgacta tgtcgaagcc
    17701 atccacgcca tcggcaaaga tgcggcagga caaggcgttt acgaaggcaa cggcatttcc
    17761 gtcaaactct ccgccataca tccgcgctac tcgcgcgccc aacacggccg tgtgatgggc
    17821 gaactgttgc cgcgcctgaa agagctgttc cttttgggta aaaaatacga tatcggtatc
    17881 aacatcgatg ccgaagaagc caaccgtctg gagctgtctt tggatttgat ggaggcactc
    17941 gtttccgatc ccgatttggc aggctacaaa ggcatcggtt tcgttgttca agcctaccaa
    18001 aaacgttgtc cgttcgttat cgactacctg atcgaccttg cccgccgcaa caaccaaaaa
    18061 ctaatgatcc gcctcgtcaa aggcgcgtat tgggacagcg aaatcaaatg ggcgcaagtg
    18121 gacggcttga acggctatcc gacctacacc cgcaaagtcc acaccgacat ctcctacctc
    18181 gcctgcgcgc gcaaactgct ttccgcgcaa gacgcggtat ttccgcaatt cgccacccac
    18241 aacgcctaca ccttgggcgc aatctaccaa atgggcaaag gcaaagactt tgaacaccaa
    18301 tgtctgcacg gcatgggcga aaccctgtac gaccaagtcg tcggcccgca aaacttaggc
    18361 cgccgcgtgc gcgtgtacgc cccggtcggc acacacgaaa ccctgctcgc ctacttggtg
    18421 cgccgcctgt tggaaaacgg cgcgaactcg tctttcgtca accaaatcgt cgatgaaaac
    18481 atcagcatcg acacgctcat ccgcagcccg ttcgacacca tcgccgaaca aggcatccac
    18541 ctgcacaacg ccctgccgct gccgcgcgat ttgtacggca aatgccgtct gaactcgcaa
    18601 ggcgtggact tgagcaacga aaacgtattg cagcagcttc aagaacagat gaacaaagcc
    18661 gccgcgcaag acttccacgc cgcatccatc gtcaacggca aagcccgcga tgtcggcgaa
    18721 gcgcaaccga ttaaaaaccc tgccgaccac gacgacatcg tcggcacagt cagctttgcc
    18781 gatgccgcgc ttgcccaaga agcggttggc gcagccgttg ccgcgttccc cgaatggagt
    18841 gcgacacctg ccgccgaacg cgccgcctgc ctgcgccgtt tcgccgacct gctcgaacag
    18901 cacacccccg cgctgatgat gcttgccgtg cgcgaagcag gcaaaacgct gaacaacgcc
    18961 attgccgaag tgcgcgaagc cgtcgatttc tgccgctact acgcaaacga agccgaacat
    19021 accctgcctc aagacgcaaa agccgtcggc gcgattgtcg ccatcagccc gtggaacttc
    19081 ccgctcgcca tctttaccgg cgaagtcgtt tccgcattgg cggcaggcaa caccgtcatc
    19141 gccaaacccg ccgaacaaac cagcctgatt gccggttatg ccgtttccct catgcacgaa
    19201 gccggcatcc cgacttccgc cctgcaactc gtcctcggcg caggcgacgt gggtgcggca
    19261 ttgaccaacg atgcccgcat cggcggcgtg attttcaccg gctcgaccga agtggcgcgc
    19321 ctgatcaaca aagcccttgc caaacgcggc gacaatcccg tcctgattgc cgaaaccggc
    19381 ggacaaaacg ccatgattgt cgattccacc gcacttgccg aacaagtctg cgccgacgta
    19441 ttgaactccg ccttcgacag cgcgggacaa cgctgctccg ccctgcgtat cttgtgcgtc
    19501 caagaagacg ttgccgaccg tatgctcgac atgatcaaag gcgcgatgga cgaactcgtc
    19561 gtcggcaaac cgattcagct cactaccgat gtcggccccg tcatcgatgc cgaagcacag
    19621 caaaacctgt tgaaccacat caacaaaatg aaaggcgttg ccaagtctta ccacgaagtc
    19681 aaaaccgctg ccgatgtcga ttccgaaaaa tccacgttcg tccgccccat cctgtttgaa
    19741 ttgaacaacc tcaacgaact gcaacgcgaa gtcttcggtc ccgtcctgca cgtcgtccgc
    19801 taccgcgccg acaaactcga cagcgtcatc gaccaaatca acagcaaagg ctacgccctg
    19861 acccacggcg tacacagccg catcgaaggc acagtacgcc acatccgcag ccgcatcgaa
    19921 gccggcaacg tttacgtcaa ccgcaacatc gtcggcgcag tcgtcggcgt acagcccttc
    19981 ggcggacacg gtctgtccgg cacaggcccc aaagcaggcg gttcgttcta cctgcaaaaa
    20041 ctgacccgca tccccgaatg ggttgccccg accctgagcc aaatcggaca ggcagacgaa
    20101 gccgcgctca aacgcctcga agcactggtt cacaaactgc cgttcaacgc cgaagagaaa
    20161 aaagccgcag cggccgcttt gggacacgcc cgcatccgca ccctgcgccg tgccgaaacc
    20221 gtccttaccg gcccgaccgg cgagcgcaac agcatctcat ggcacgcgcc aaaacgcgtc
    20281 tgggtacacg gcggcagcct cctgcaagcc ttctgcgccc taaccgaact tgccgcctcc
    20341 ggcgtacagg cagtggtcga acccgacagc ccgctggctt cctacaccgc cgacttggaa
    20401 ggtctgctgc tggtcaacgg caaacccgaa accgccgaca tcagccacgt tgccgccctg
    20461 tcgcctttgg acagcgcgcg caaacaggaa cttgccgccc acgacggcgc actcatccgc
    20521 atcctccctt cggaaaacgg actcgacatc ctgcaagtgt ttgaagaaat ctcttgcagc
    20581 gtcaacacca cagccgccgg cggcaacgcc agcctgatgg cggttgccga ctgattttac
    20641 cgaaatcccc gagcgcggcc cgtgaaccaa tgccgtctga aaacctttca gacggc
//
LOCUS       DAXHTR010000016        19795 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.016, whole genome shotgun sequence.
ACCESSION   DAXHTR010000016 DAXHTR010000000
VERSION     DAXHTR010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19795
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.016"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(203..278)
                     /locus_tag="ACFNML_000423"
     tRNA            complement(203..278)
                     /locus_tag="ACFNML_000423"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(243..245),aa:Asn,seq:gtt)
     gene            complement(371..730)
                     /locus_tag="ACFNML_000424"
     CDS             complement(371..730)
                     /locus_tag="ACFNML_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HFC6391862.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(753..1397)
                     /locus_tag="ACFNML_000425"
     CDS             complement(753..1397)
                     /locus_tag="ACFNML_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HFC6391863.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRA"
     gene            complement(1399..1935)
                     /locus_tag="ACFNML_000426"
     CDS             complement(1399..1935)
                     /locus_tag="ACFNML_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HFC6391864.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDTKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(1947..3044)
                     /locus_tag="ACFNML_000427"
     CDS             complement(1947..3044)
                     /locus_tag="ACFNML_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6391865.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(3047..4483)
                     /locus_tag="ACFNML_000428"
     CDS             complement(3047..4483)
                     /locus_tag="ACFNML_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6391866.1"
                     /translation="MENSLIIGLALAVLLVLLVMRAKQGKKTPKRKSQGVGNTQGQPP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="ACFNML_000429"
     CDS             complement(4703..5485)
                     /gene="bioC"
                     /locus_tag="ACFNML_000429"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFC6391867.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVRLTYHPLFFIARYSAAGRQ"
     gene            complement(5473..6120)
                     /locus_tag="ACFNML_000430"
     CDS             complement(5473..6120)
                     /locus_tag="ACFNML_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl esterase BioG family
                     protein"
                     /protein_id="HFC6391868.1"
                     /translation="MKTKFYNHQGGHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPCA
                     VFKGTLENLTENTRLKFERRMCGDKASFEDYQQFPARPFDEIHQELIALFAMIGQDRR
                     ADLIRWTNALVGSGDKIFMPANQHRYWTPRCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="ACFNML_000431"
     CDS             complement(6130..7272)
                     /gene="bioF"
                     /locus_tag="ACFNML_000431"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFC6391869.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFSRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKNTSRIRLSLTADMTTDEVRQFAVCL"
     gene            7495..8571
                     /locus_tag="ACFNML_000432"
     CDS             7495..8571
                     /locus_tag="ACFNML_000432"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFC6391870.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(8778..10244)
                     /locus_tag="ACFNML_000433"
     CDS             complement(8778..10244)
                     /locus_tag="ACFNML_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HFC6391871.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFIGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFFAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(10442..11365)
                     /gene="speB"
                     /locus_tag="ACFNML_000434"
     CDS             complement(10442..11365)
                     /gene="speB"
                     /locus_tag="ACFNML_000434"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFC6391872.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGIIDPAHSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(11464..13356)
                     /gene="speA"
                     /locus_tag="ACFNML_000435"
     CDS             complement(11464..13356)
                     /gene="speA"
                     /locus_tag="ACFNML_000435"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFC6391873.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYAATVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(13523..13807)
                     /locus_tag="ACFNML_000436"
     CDS             complement(13523..13807)
                     /locus_tag="ACFNML_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HFC6391874.1"
                     /translation="MKKLLLAAVVSLSAATAFAGDSAERQIYGDPHFEQNRTKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="ACFNML_000437"
     CDS             complement(13958..15766)
                     /gene="aspS"
                     /locus_tag="ACFNML_000437"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFC6391875.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSARNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPVMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALGVDLGDFPRMPYSEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIARTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGAENGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(15824..16525)
                     /locus_tag="ACFNML_000438"
     CDS             complement(15824..16525)
                     /locus_tag="ACFNML_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HFC6391876.1"
                     /translation="MTEPEAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQSGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(16668..17816)
                     /locus_tag="ACFNML_000439"
     CDS             complement(16668..17816)
                     /locus_tag="ACFNML_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFC6391877.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="ACFNML_000440"
     CDS             complement(17977..18240)
                     /gene="rpsT"
                     /locus_tag="ACFNML_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFC6391878.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            18543..19685
                     /locus_tag="ACFNML_000441"
     CDS             18543..19685
                     /locus_tag="ACFNML_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFC6391879.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LNPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
BASE COUNT         5090 a         5114 c         4600 g         4991 t
ORIGIN      
        1 caatgccgta ctggtttttg ttaatccact ataaaatcga gtttatccta gctgtccagg
       61 acagccccta ttttttcata accatcacga aaggaatttt gcaaagattt tattccatct
      121 caaaacaatc atctcaaaaa tgcgtttctg accgccggta aaacaaaacc ctctaagaaa
      181 atacttagag ggttttgaaa tttggctccc cgacctgggc tcgaaccagg gacctgcgga
      241 ttaacagtcc gtcgctctac cgactgagct atcggggaac aagcgcgaat aataaaacag
      301 aaataccgaa cgtgtcaata taaattgaca atcgattgca ctttatctgc aagcccgcaa
      361 tctttatatg ttatatggct tccgcaatat gccttgccgc acgtttggca tccaatagga
      421 ttaaaccaag tctgccggtt tcttttgcca ccagcaccaa cacggcatct ttacccgcct
      481 gacttaaaag gatatagcct gattttcctt taatcatcac ttgttccaat tccccgcagg
      541 cgagttcctg caccgagcga ctccccaaag caagcaaagt ggcagaaatc gcccctaccc
      601 tgtccgaatt caaatgtgaa ggaagcattg tcgccatcgg caatccgtcg gttgagataa
      661 cggcagacgc gataatatcc gtagatgtat tgtttaaatc ttcaaggatt gaaatcaata
      721 attgctgcat aacccctcct ctctcccaag ttttacgcgc ggttgctgta acggcggtat
      781 aaaatcctta ccaaagtaac aaatgcctct ttgcccaaat cgggaatgcc gccgataacc
      841 aaaataaatt tggttgaacc gatatacaat gggaaaaatg tcaattcgct ctgaccggaa
      901 ggatcgcaaa cgccccaagc gttattgttg atatacaggt tgttcttaat cagcagccgg
      961 tatttctttt ccatctgtgc gacttctgcc gccaacaacc ccaactcttc cgccgcctca
     1021 tgatggaaat tggcgttggc aagatacaga ccgttccgat cgaccaataa cgccttaccg
     1081 ctgccggaca attgttccat cagcaacggc aattgctcgt ccgacaaatt gatgccgtct
     1141 gaatgaccgt tttcatcgcc atagaggaat tcgagttttt gcaaacggta caacaggttc
     1201 aaagcggtat cgatgtcggc ggtgtccgcc caagtaagca gcttctcact gctgaccatt
     1261 tcgtccgcat ctgctttcaa caggctgtgc aacaaagttt taccggcact gggggcatcg
     1321 ctggatacgg cataaaatgc accggcagga gtcaggcggg gatataaatt tgcttgtagt
     1381 gaaagtgttg attccatatt aaacctccag tcccggatca atagaaaata acattgcgct
     1441 aaccaattgt tttacgtcat cttccttacg ggcatcaatt tcaaaaaccg gaacattaag
     1501 attatgtttt gcaagatatt tgtgatacac gtcgataccg ggctgagagc gtatatccat
     1561 cttggtaata ccgacaacga cgggtgcctt ctccagcagc cctcgaaacg aatgtaaaaa
     1621 gaattccaaa tctttcaacg gattggttcg ggcattatct aaaagcaaga ccaaacccat
     1681 actgccttgg cttaagattt cccacataaa gttgaaccgt tcctgaccgg gcgtaccata
     1741 taaatggact ttggtatcct catccaagct gatggccccg tagtccatcg ccactgtcgt
     1801 attccttttc ctatccaaag tcatatcgga tgcggaagca tcggtctgaa cgagtgcttc
     1861 gtccgaaata gccgcaatgg cagtggtttt ccctacgccg acaggtcctg tgaaaataat
     1921 tttattttct ctcatctctc cgcctcttag ctgcccaaca gttttttcat cagcctttgc
     1981 agaaggccgc gcgactggga ttgggatggt gttgtgattt tttccgcttt tttcatcatt
     2041 tcactatcag aggcagaatc ggctccgata tttattttat ccgccatatc ggagtatgcc
     2101 tgttgtgaaa ccgtttttaa atctaccgac aaaaacccgg ttgtataggt tgccgcaaga
     2161 taattcagaa tatcattgag gtttaaaggc atcactttat acaacacgtt aaggttgacg
     2221 gatgccttgg tcagaaatgc cgacaagcgt atcgaccccg gcacatttgc caaccgggtc
     2281 aggtttggcc aagatttcaa cgtaaacgga gtatcgggag aaatcggata aatcaacctg
     2341 ccctgcgctg tccaaatgga aaactgccac atacaggaca taatgcctac tttagccttt
     2401 tcgcgccatt gcgggttatc gggaacagtc ttgcagctga cctgcaaatt ttcgtctttg
     2461 cacaattctt cgagtttttg cacactttct gtcagcaaaa cccgttgtat cgaggggaaa
     2521 acaataagga ccggcttatt tccatgcaag atagcgatgt cctgcctgtt cttttccgca
     2581 aaccgcaacg cccccaataa tcctttattc gggttaaact ggcgtatcgt aaccgtacgc
     2641 tgcacattcc cgttattttt cgccgaccgg tctgcgggtg caataaacga tttcccataa
     2701 acattctcgc cctgcaacaa tttgcggagc ataggaaaca atgtttcaaa ccgaatcggt
     2761 ttgggcaggt agggaacttc agaatcggga actttctccg aacagacggc gacgggtata
     2821 tccttataac gctcggcaag ctctttccaa agttcaaaac cgccctcggc atcggtatcc
     2881 gccaaaacca aatcgggcac ggcactgccg tctgaagggg atactgtttc ataacgggtg
     2941 gtattgtgca ttttgaatgc cattttgaaa acggattcct gctgcgccgt catccccgcc
     3001 aacattacgc gtactgtttt aattttcggc agttgaactt ccatttttat tttccgtacc
     3061 gttttatttt tttaatattt tgattcatac gctgcagcag ccggctcatc agcataacga
     3121 cctcttcagg aagcctgtcc gcacgttccc tcaataccct taaaaactgc cccaacctat
     3181 cccaatcttc agtacgttca taaatatcga tcaacgtaat ataaagctgg gactcgtcgg
     3241 gatatttcaa taccgcctgc tccaacacat ccattgccgc ttcaatctga ccatacatca
     3301 gcaacgactc tacttcctta accgcatcgt ctgccggaga cgaaccggtg ttgatcaacg
     3361 aagaatcttg aagcaccaaa tcccgatgtt gcgatttgaa tttctgtata tttttcggca
     3421 gatacccgtg ccccataccg atgtctttga tttgccggtc gttcggccct ttttccaaat
     3481 catcgaaaac ctcatggtaa cccaggctgt acccccaccc cagcatccgc tctttaacct
     3541 gcctgccgta gttgcccaac gtttggtaaa gtttccacaa atgtccggca aaacggtcta
     3601 tgtccgcgta ttggtaatcg agtttcaacg catcgataat caggtttgca ggtttttcgg
     3661 aagtttggat ggcacggtcg tattgtttcg atgccgtttc atagctgact ttgtctttaa
     3721 ggattttcgc accttgatcg gcacggacca aaccggcaat cgcaccgatt tcctcttgac
     3781 tgattccgga cacgtctttt ttgccccgca caatcgggat gcgcttgatt tcttcggctt
     3841 cataagcctt accgccggca tccggcgggg gcgatgcgga agctgccgtc gcagaaccgc
     3901 cttccgcacg tttttcaatc tcctgagtac cccatcccaa accttcttcc gccaagacgc
     3961 ggatacgcaa atggtttgaa tcgcgctgta atgcctgttc gatatatttt gccaaaagtt
     4021 cggaaggaat cagtttgccg tatttttgca gattgtctgc caaaacatcg acatccccca
     4081 cttcgagatt gatatcgagc agctcgcgga taaggttttc aggtttcgct tcaccatccg
     4141 gaatgccgtc cagataggca gccaaagatt cggcagcctt gccctgataa ccgaattgct
     4201 tataaacctg atactccgta agcggatcga cttcttgcgc ggatacggcg gcggatgcgg
     4261 tctcggcact ttcgttccaa gaccagtcgg gttgcgtgcc gtctgaaacc gattgtccaa
     4321 tctgatcgac ccagtccgaa tcattgcttc cgggcggctg cccctgcgta ttgccgacac
     4381 cttgggattt gcgcttcggt gttttcttac cctgcttcgc acgcataacc aaaagcacca
     4441 gcaataccgc cagtgccaga ccgataatta atgagttttc caaaggatac tcccgatacc
     4501 gtaacgagcg gatagctgac cgcctgcaac ggccattgtc cgcttttatt attaacgatt
     4561 tactgaaata gacttgtaag ttttaaatca taccataatt taacgtttaa caatatgcct
     4621 tctgcacaag cctcgccata ttacctttta cccacatcag tatcaatacc cgatataaaa
     4681 ataactttgc ccataagctg ccttattgcc tgcccgccgc agagtagcgc gcgataaaaa
     4741 ataacggatg ataagtcagg cgcactttgc ccgacggcat actgaacgcc gacaagtaat
     4801 cgccaataaa tccattgaga ttttttcttg tccaattttt ttggttcgtg gctgttacgc
     4861 ctgtatattt aaggtgtttg agtacatcta acggcgtatc aaagtctagt attaccttaa
     4921 aatcctcaca ccataaaagc tcaaaatctt tggctaacca agtctgccat tgggataaag
     4981 tcgggtaatt taagcctata tttgtaattt ggcggacttc ttttaaattg tctttgccaa
     5041 aggttgcaac cgccaataat ccgtttgctt tcaagcctgt tttgcaatgg gcgataaaag
     5101 cgtcgggttg atgaaaccat tgcacggcag atgcgcttgc gattaagtca aattgtcgtt
     5161 gaaaaggaaa gttttccgca tcgccgcaat aaaaatcaaa ggattgcggc agtttttcag
     5221 ccagttgggg ctgcacatcg cacaaatcat taaataacca ataattcgct gaaatctgtt
     5281 tttgcagcaa ggcactcaac attcctgagc cgcagcccaa ttccaacacg ttttccaatg
     5341 gcatatccgg caaataatct tgcaaatgcg ccattagatt aatcgtcatt ttttgttgga
     5401 ttaaggcgtg ccggtcataa tcgtttaatg ctttttggaa agcctgccga atgcgcgatt
     5461 tatttatggc agtcagtgat tccatagtgc cgaccaatgg gtgaatcttg aaaacaggta
     5521 atgtccgccg tcaatttccc gaacggtgca acgcggtgtc caatatcggt gctgattggc
     5581 aggcataaaa attttatcgc ccgatccgac caaggcattt gtccagcgaa tcaaatctgc
     5641 gcgcctatct tgcccgatca tcgcaaaaag tgcgataagt tcttgatgaa tttcgtcaaa
     5701 cggacgggcg ggaaattgtt ggtaatcttc aaaagatgct ttatcgccac acattctgcg
     5761 ttcaaatttt aaacgggtgt tttccgtgag gttctccaat gtgcctttaa aaacggcgca
     5821 agggataccg aaattatcat cgcaaggcaa acctgtgcca ttcactgccg ttgcggattt
     5881 taatcttatt ccttgcaatg ccctctctgc cgcccaaacg cccattgacc acgccaccaa
     5941 acggatgtgc cgataggcgg aaaaatcaaa atccaaattt aaatcttgat aatcatagca
     6001 aatcaataaa tcgtgatttt ccggcaaaat caaatgattt acagcatcgg gcggcgttcc
     6061 ccaacctgca aaatacagga ttaaatgtcc gccttgatga ttgtaaaatt ttgttttcat
     6121 atcatatcct tacaggcaca ccgcaaactg ccgcacttca tccgttgtca tatctgccgt
     6181 taaagacagg cggattctgg atgtgttttt gggtactgtc ggtggtctga tgggcaggca
     6241 ataataaccc tgcctttgca ggtattccgc tttggcaagg gtggcttcat tcccgcctaa
     6301 aatatagggg acgatacagg tttggctcgg cattatttgc gtccgatgcg ccacttcccg
     6361 ccgtaaaaat gcgcttaact gctcaagatg gcttctttct tttgagaatt gcggcaatcg
     6421 ttcaaaaata aaataagtcc aagccacatt aaacggcggc aatgcggttg aaaaaatcaa
     6481 tgggcgcatt tgattaatca aacattcttt caatacttgg ttgcagacgg catacgcccc
     6541 caccgaggct aaggctttac cgaaagtgcc gaccaataaa tcaatctcgg caatcaaatt
     6601 atcccgttcg gcaatcccca atccgttttg cccataaaca ccgattgcgt gggcttcatc
     6661 cacataaaga taagtattgg gaaactgttt ttttaattgg acaagctgtt tcaaatccgc
     6721 cacatcgccg tccatactga aaacagattc ggtaacgata aaagtgcggt caaattttcc
     6781 gacgtttttt tcaagcagat ttttcaaatg ttcataatca ttatgacgat aacgggaaaa
     6841 cgcacaccgg ctcaaacgga tgccgtcaat catactggcg tgaacaaatt tatctgccaa
     6901 aatcaaactt ttcgtcgtcg tcaaagcagg caaaataccg agattggcgt gatagccgct
     6961 gttgaacaat aacgcgcttt cccgttggaa acgttgtgcg acaagctctt ccaaatcggt
     7021 ataaatagga aagttgcccg ttaataaacg cgatgaagaa ctggtaaaag agggaaaatt
     7081 accgccgtat tgctgcaaaa aagaccggcg caagttttca tctgatgcca aacccaaata
     7141 atcattagac gacatattca gcattttgca gttttcccgc gtaatatacc gcccttgatg
     7201 aatcaaatcc ggaatcgaac gatattggtt ttgcgcgccg agttgttcaa gctgttgttt
     7261 aaaaaccttc attatgatgt aaatattcct gagttaaagc ctgaaccaac ccagcatcaa
     7321 acaatcaagt aatgcccttt taataaaaac cgttttgccg ccgacctttg ccacacccct
     7381 gccttcataa tccaaggcgg aaatttccgc gacatttgtt tccgttgcca ttacccaatc
     7441 cgtccgcact gtaccgaagc gcatattttc gcacaaattg gtacggtttc aggtatgatt
     7501 tgccgaaaac ctatcggaca cacaccatat tttgatatga ataaaacaat ctgccgcact
     7561 gccgcactat tgataagcgg tttttcatac gcaaacaccg tcattcccga tgtctccccc
     7621 gtcgcacaag gtcaacatgt cttcattaac atccctcagc aacgcctgtt cctctacacc
     7681 gacggcaaac tgaccaaggt ttatcccgtt gcagtaggtc gggcgatgac gcaaaccaat
     7741 ctaggcgaac ataaaatcgg tgcgaaagcc tataaccctg tttggtacat ccccaaatcc
     7801 atacagaaag aacgcggcga cggcgtaaaa accatagccg ccggcccgga caaccctttg
     7861 gggccggttt ttgtccgctt aggcgacccc aaactcggtt tgggcataca cgggaccaat
     7921 gcgccggcca gcgtccccgg ggttcggagt cacggctgcg tccgcatgaa atcgcccgac
     7981 gcgctcgagt ttgccaaaac catcgccagc ggctcgcccg cctccgtcat ctatcaaatg
     8041 gcggggctca atgaagatgc ggatcgcaac ctgtggcttg ccgccttccg cgacccttac
     8101 ggtaagaaca accttgacat cgcctctctg aaaaaaagta ttgcgcaatg ggcaaaaaca
     8161 cagggtaaaa ccatcgcgcc cgagaaagtc gatgctgtac tcaaagaccg caccggatcg
     8221 gccgtctgcc tgacctgcgg caaaaacggc aagatgaaga tgccgctcaa atcgctggcg
     8281 tggatacagg gttcttcctc atacagccaa gctgaagtca ttgaacaaac tgaggaaacc
     8341 aactctgccg aaatatccga gacacgcacg cccgaagtgc ctgatgtaca cacgcccgag
     8401 gcacaaccgc atttaaatac ccaatccgac ggcacgccga ctgcctatac cgaaccggct
     8461 gccgattcat cgccgcaagt agaaacacct gatcaggctg cttccgagcc ggttgatgta
     8521 ttattttcaa tagatgtgat acggcaggga aatttgcgtt taggtaattg aataaccttc
     8581 tgattattta atgcttatgt ttatcagaag ttgataggcg gtttggtttt tgtttggctt
     8641 ttcctacacc gccgcctgcc gctttctgca acattcaagc gcacaaatat gccatctgaa
     8701 ggctttagac ggcatatttc acgataatag tcgcccaatc aaataaacac agattggaaa
     8761 tttattttat ttttccctta ccaatacccc agaactttcc accaaatgct gccgataacg
     8821 aaaaaaatca gaaaattgac cacgctcatg ataaaacccg ccttccacca ttctcccatt
     8881 gtggtgtagc ccgaaccgaa aatcacaggc gaagtaccgg tcgcataatg agtgagggtc
     8941 atcataatgt tggatgcggc cgccatcatc agcgcggtcg gcatcgccgg ggcattcagt
     9001 gaaacggcag cagcgaaaaa tgcgccgaac atagcggtaa tatgtgcagt agtactggca
     9061 aacatataat gcgcatacat ataagcaagc acgaggatta cgcccgcagc cgtgccgcta
     9121 acgcccaaac cgccgacact ttccgccaac actccggaga accatttaat cagtccgagt
     9181 ttatttaaaa atgcggccat cataatcaat gcgccaaacc aaataatcgt atcccacgcg
     9241 cttttttctt tcaaaacatc gtcccaagtc aatacaccgg aaagcaaaag caggcttaat
     9301 ccgataaatg cggtggcggt ggcgttgata ctaaaagcgt gattgccggt aataagggcg
     9361 ggaacatctg cccacaacag caacaagata ccgaaaatga ccgccataat gatttcgtct
     9421 gccgacattt tacccatctc cctcagacgg tctttggcaa attgaacggc attgggcgtt
     9481 tctttaattt caggcggata caaaaaatat aaaatcaaag gcataacgaa aaaggcgata
     9541 acgccgggaa cagccattgc ccacgcccac gccccccaag aaagacggaa actactgcct
     9601 aaattttcgg caatcaagtt gacgattaaa gggttggggg cagttgcagt aataaacata
     9661 gccgacgaaa tgggattgga atgatagttg accaaagcca aatatttacc catcttgcct
     9721 tctgtgcctt ttgcgggatt ggagccgtaa ctgccggcaa tcgactgcat aatcggatgt
     9781 ataatgccgc cgccgcgcgc ggtattggaa ggggtaacgg gagccagcag cagttcggaa
     9841 agagcgagac tgtaaccgat gcccagcgtt tttcttccaa aaacggcgat aaacaaatat
     9901 ccgatacgca tccccagccc tgttttgagc aaaccgcgcg aaatcataac tgcgatggca
     9961 atcagccaaa tcaacggatt ggcgaacgca ctcaacgcat cgctcatcgc cgcacccggt
    10021 ttgtcggcgg ttacgccggt tactgcgacc aacccgacgg caataatcga cagcgcaccc
    10081 aacggcatgg ccttgccgat aatggcggca atcacaccga taaacatggc cagcagcgtc
    10141 caagcctgag gcttgacccc gtcgggtacg ggcagtgcca aaaccaaggc gcacaatact
    10201 gcggcaatgg cgaggggtat cggtttgaaa cccaatttca tcatattgac ctccgtaaaa
    10261 aagaccgtcc cgaaaaatcg gaaaaataat atttaactaa ttgttttata agatatattc
    10321 cgatatttca ccgtctttcc gatatgcggc tccgggcaac ttttgtttca gtatttgaat
    10381 tttcattaga ctgaatacgc cgtttgaacg gcacggcgaa aacccggggg atgccggacg
    10441 ttcagtcctt tttcgcacct tgaaggtaaa gcatttccaa ggcaattgtg gcaccggcca
    10501 aagcggtaat gtcggattgg tcgtaagagg gggcaacttc tacaacatcc ataccgacga
    10561 tgtcgagatc cgtcagccca cgtaggattt ttaatgccct gtcgctgctc aagccgccgc
    10621 atacgggcgt accggtccca ggggcgaacg acgggtccag gcagtcgatg tcgaaagtca
    10681 ggtaaacggg catattgccg acggtttctt tgattttacg gacggtctct tcaacactgt
    10741 cttcattgac ttggggggcg gtcaacacag taaaaggcaa ttttttactg tgttcggtac
    10801 gtatgccgat ttgcacggaa tgtgccggat cgatgatgcc ttccttgggg gcggtataaa
    10861 acatcgtacc gtggtcgtat tcgctgccgt tgtcgtaggt gtcggtgtgc gcgtcaaaat
    10921 gaatcagtgc gagtttgccg aaatagcggg cgtgggcgcg caacaacggt agggtaatga
    10981 aatggtcgcc gcccaaactc aaacagcgtt tgccggaaga aagtaatttg ccggcgtgcg
    11041 cttccatttt ttcgacaaaa tccctgctgt cgccaaaaga aaaaaccaag tcgccgcaat
    11101 caataatgtt caggcgttcg cgcacatcaa atgtccatgg aaacctgcgg tgctcccaag
    11161 cgaggttgac ggaggcgcgc cggatggctt caggaccgaa acgcgcgccg gaacgccctg
    11221 aaaccgccat atcataaggc acgccggtaa taacccaatc ggcatgactt tcatacggca
    11281 taaaattaag cggcaggcgc aaaaacccga aattattgga aacgagggag ttgtcggttt
    11341 gtcctgccag tgtgctgtat tgcatcgtaa tgattccttg taattggttt caatcggtcg
    11401 tgatgattgg tgtttgagta agaaaatcgg gcttcagacg acatatccga tgccttgatg
    11461 cgtctattcg tcttccaaat aggtataacc attaagcccc gcttcgagtt cttttaagaa
    11521 agacatagcc tgcgaggcag gaaggtctga atgttcgatt tgttcgcgat agcgtttcat
    11581 cagctctttc ggatcttgat aaacgtattc gagcatatcg gcaacggtgt ttccttcatc
    11641 gtaatcgatg acggtaaatt gtccgtcttc ccctacaaca acatcggcag tggcagtgtc
    11701 gccgaaaaga ttgtgcatat tgccgagtat ttcctgatat gctcccacca taaaaaagcc
    11761 taaaagcggc ggctcttctt cgggataatc aggcataggc atcgtaccgg cgatgccgtc
    11821 gccgtcgatg tagtggtcaa tcgtaccgtc tgaatcgcag gtaatgtcca acaacacggc
    11881 gcggcgcgca atcggttcat tcaaaccggt aatgggacaa acagggaaaa gttgatctat
    11941 gccccaagca tcgggcaaag attggaagag tgagaaattg acatacagct tatcggcaaa
    12001 acgttcttgc aattcgtcaa taatggttcg gtgagaccgg tgtttttcat taaacaattc
    12061 gccgacttca tgacagatat ttaaatacag ttgctccgcc cacgcacgtt gcgccaaact
    12121 caacagcccg acattatact gattatgcac atcggcaaga tcaaactgcc cttcgtgtat
    12181 ccagctgcgt aaggaacgtt tttcccgcga ggcggaaata tccgtccaag tttcccacat
    12241 actgtgcaac acacgcggtg cttcgggcga tggcgcatcc agccggcgcg gtttgtaacg
    12301 ttcaacgcct ataacattag caaccaaaac ggcgtgatgt gcggtaatgc cgcgcccgct
    12361 ctcggtaatg attgtcggat gcggcagccc gtgttcgaga caagcctggc tgatgcccca
    12421 tacgactgtg gcggcatatt cgttgaggct gtaattaacg gaacaatccg attgtgtgcg
    12481 gtttccttcg taatccacgc caagcccgcc gcctacatca aaacagcgga tatttacccc
    12541 cagtttgtgc aactcaacat aaaaccgagc cgattcgtgt acacctgtgg caacatcacg
    12601 gatgttccca agctgcgagc ccaaatggaa atgcaaaagc tgcaggcaat ccagcctgtt
    12661 tttttgtttc aaaatatcga ccagttgcaa aacttgggaa gccgacaagc cgaattttga
    12721 tttttcccca cccgaagact gccattttcc cgaaccttgg gaagccagtc tggcgcgcac
    12781 acccaaacgg ggcttgatgc cgagtttttc cgcctcttcc aataccattt gtatttcgga
    12841 cagcttctca atcaccaaat aaacctgatg ccccagtttt tcgcccatca aggcgaaacg
    12901 gatatattca cggtctttat agccgttgca gacgattaat gtttgccggg tgccggcgtg
    12961 tgccaaaacc gccatcagtt cggctttaga accagcttcc aaaccatgcg gttgtccgct
    13021 tgacataagc gattcgatga cgcggcggtg ttggttgacc ttgatagggt aaaccaaaca
    13081 ataaccgccc ttatagccgc actcttcccg tgccgtctga aaggcgcggt tgatgtcgcg
    13141 gaggcggtgt tcgaggattt gcggaaaaca aaacaaaaca ggcaggcgcg cctgatgttt
    13201 ttgttgcacg gcttcagtca gtttttgcag tgaaacagtt tgattgtgtt gcgagggatt
    13261 ggggcggacg atgatttcgc cggaatcgtc aacatcataa taacctatac cccaatgatt
    13321 aatgttgcac acttcacgga tggtaaggat aggcataata aacctgctcc gtctgtcgtg
    13381 ttgaaaagga atgattataa caaatcagcg tgaaatgtca tttttttaat aagaaaagcc
    13441 tgcctcatac ctgatgagga acaggcaaaa tgccgtctga acgcttcaga cggcattttg
    13501 gttcagcttg ccatcaaagg agtcagcgat cgagctgctc tttgatgatt ttcaggtcgg
    13561 ggtaagacaa cacgatgtcg tattcgcggc cgtctttata ggcttccact tccagcacag
    13621 gcttacccca atggtcgtcg gcatcgacat cgtgaacctg ataaccacgc tgttccaaca
    13681 ttttcacggc ttttgtgcgg ttttgttcaa aatggggatc gccgtaaatc tgacgctcgg
    13741 cagaatcgcc ggcaaatgcg gttgcggcac tcagggaaac aacggcggcc aataacagtt
    13801 ttttcatttt cagtcctttt tttatcggtt gattgaacaa gatgtgtttt tcaataccgc
    13861 cattaaaaca cagcaaaatt aggtttgaat tagagggaag tcaagaagcg taaatgccgt
    13921 ctgaaaaaaa cagagccgtc aaacggctct gttttcctta tgcttctttg gtttcagccg
    13981 ccttttgacg caggcgcaag ctcaattcgc gcaactgctt gtcgtccacg ctgttgggcg
    14041 cgttggtcag caggcattga gcacgttgtg ttttcgggaa ggcaatcacg tcgcggatgg
    14101 attcggctcc ggtcatcagc gttaccagac ggtcgaggcc gaatgcaagg ccgccgtgag
    14161 gaggcgcacc gaatttcagg ttgtccaaga ggaagccgaa tttctcttgt tgctcttcag
    14221 ggctgatttt cagcgcggca aacactttct cttgcacgtc tgcgcggtgg atacggatag
    14281 agccgccgcc gatttcccag ccgttcaata ccatatcgta ggcgcgtgcc aaacaatttg
    14341 ccgggtcgga aaccatcagg tcttcatgac cttcttttgg cgcagtaaac ggatggtgta
    14401 cggcaacgta gcggtcggct tcttcgtcgt attcgaacat tgggaagtcg acaacccaca
    14461 aaggtttcca ttcgtctgtg aaatagccgt tttccgcgcc gtgttccaag ccgactttga
    14521 tacgcaatgc gccgatggct tcgtttacga ctttggtttt gtctgcaccg aagaagataa
    14581 tatcgccgtt ttgtgcgccg gtacgcgcga tgatttcctt cagggcattc tcagacagga
    14641 attttacgat tggagattgc aggccgctgt cttcgccgtt ggagaggttg cccacatcat
    14701 ttactttgat gtatgccaga cctttcgcac cgtagatgcc gacaaatttg gtgtattcgt
    14761 cgatttcttt gcggctgaat ttcgcgccgt tcggtacacg cagagcgact acgcggccgc
    14821 ctttcatatc tgccgcgcca cggaagactt tgaattcttc cgttttcatc aggtcggtca
    14881 actcggtgaa tttcaagttg atacgcatgt ccggtttgtc agagccgtag tagaacatgg
    14941 cttcagagta aggcatgcgt gggaagtcgc ccaagtccac gcccagcgcg tctttgaaga
    15001 cttgtttcgc cataccttcg gtgatgtcca tgatttcatc ctcgtttaag aacgaggttt
    15061 ccaagtcgat ttgggtaaat tcaggctgac ggtcggcacg caggtcttcg tcgcggaagc
    15121 acttggtgat ttggtagtaa cggtcgaaac ccgccaccat caacagttgt ttgaataatt
    15181 gcggcgattg cggtagcgcg aaaaactcgc ccggatgaac gcggctcggc acgaggtagt
    15241 cgcgcgcgcc ttcaggcgtg gagcgggtca gcatcggggt ttcaatgtcg atgaaacctt
    15301 gcgcgtccaa gtagcggcga acgcccatag ccacttggta acgcaggcgc aggttgcgct
    15361 gcatcaccgg acggcgcagg tcgataacgc ggttggtcag gcgaacgttt tcgctgatgt
    15421 tttcatcgtc gatttggaac ggaggcgtag cggcggcgtt taatacttcg atttctttgg
    15481 caagaatttc gattttgccg gaaatcattt tgtcgttggt tgtaccttcg ggacggttgc
    15541 gtacgcggcc ggtaatgctc aaaacgtatt cgttgcgggc ggaatcggca gcagcaaacg
    15601 cttccggagt gtcggggtca atcacgactt ggacgatgcc ttcgcggtcg cgcaggtcga
    15661 taaaaatcac accgccgtgg tcgcgtcggc ggtgtaccca gcctttgacg gttacggttt
    15721 ggtctaagta ttgctcgctg atcaggccgc aatagttggt acgcataaat tcacctttta
    15781 ttgatttaaa ctgaaaacag aaaatgccgt ctgaacggcg gctttattgt tgttcgggca
    15841 aatccgcctt ttcagacggc ataggtcctg ccaatgtttt gacgggcagg tcgtcaggga
    15901 tgaccatacc cagcgaaatc acatatttca atgcttcatc cacgctcata tcgagttcgc
    15961 gcacatcgct tttctttacc ataatatagt aaccgccggt cggattcggc gtggtcggaa
    16021 catacacgga aagataatcg ccgtccttcg gcaatgcggc cttaaccgca ttcgacacct
    16081 gaccggacac gaatgcgatt gtccaaatac ccgattgggg aaacggcacg agtactggtg
    16141 ttttaaacga acggctgctg tcggacagca gcgattcgga tacttttttc acactcgaat
    16201 agatggactt cacaactgga atccgcccca acaggctgtc ccacgcggca agaatctgcc
    16261 ggcccaatac gtttgcggca aacaatccgg ttacaaacaa tacagcaatg gcaacgataa
    16321 cgcccagccc cgggatattg aagcccaaaa catattgcgg ccgccattgc ttcggcagca
    16381 ggttgacgag ctgatcggac gcggaaacga tataggaaac cacccaaacc gttaccgcaa
    16441 tcggcagcca gaccaaaatg cccgtaatca gatatttttt taacgccttg gcagctttac
    16501 cgccttcggc ctcaggttcc gtcatcttgc ttgattccga caaagtccgt acaaaccgca
    16561 cattatacgc gtttgcccgg attcaaacga aatttttatc ccgccccgcc aaaccgccag
    16621 cggcttcaga cggcacggca acttgatata ccgtctgaac acgcggttca gatgccgtcc
    16681 aagtcgttga acatcaaccc gataccgata ccgttctgct tgtggttgta gtcgatcagg
    16741 ctctcgccgt aaccgtggaa tccgcgtacc acgcctttga gtttgccctt aatcggaaac
    16801 gtgtaggcgg cttcaatcgc gccgtagccc gttttggggt tgtagcgcaa tacggaatac
    16861 acattctgcc tgtcgttcag gcggtactgc agcttcacgt cgccataccc catatagtcg
    16921 gcaatatcgg gattgtcgtt tttatcgccg ctctgatcga acgcacgcac ccacacgcgc
    16981 ggaatcaccg tcaatttgcc ccattccatg cctgccatgg cgtaaatcct gttccacgaa
    17041 cgcgattcgg gacggctctg tccgttggac tggtggacaa aacccgcacc gagcatacgc
    17101 agcctgccgc cgaacggcaa atccgccttc acaggctggg tcaggaaaat ttcaggtttg
    17161 taatccgtat tgcggaacgg cgcggatttc ctgccttggt tgtaaatctg ccaatcggat
    17221 ctttgggtgt agccgaacca cagatccgcg cgggttttaa acaaatcttc ggcaattttg
    17281 cttttgaacg aaacctgcaa tttggtttcc gcacgtttct gctgtccgaa tttttcctgt
    17341 acagtcgtac cgcgcgtcgg cgaacccggg gcatagttgg gcgaattgtt gtaccagagc
    17401 ggcataaggt acatcggatt gtgttcgcgt acgcccaaca gcccgcgcaa atcgtttttg
    17461 tccaagtcgt acatcaggct caaaggcgta tagatgtcgg cggtttcgcc cgcactgtcg
    17521 gcaggaagcg catccccgcc tttttcaaca acaatgaccg cctcgccctt atccaggctg
    17581 ctgcggacgg tttccgtcag attgagtacg gctttcgact cctgcccttc ctgccctgcc
    17641 gaagacggaa gctgtgccgc aaaaatcctg tcgtaacacg ccaaacgcgt aacattgtcc
    17701 gtcaaagcgg cgcattgcag cgcggtctct ccaaaagcgg atgccatcgg caacagtcct
    17761 gtcaaaagaa tatagcgcat attccgtgta ttcatctccg cccccattgt cggcatattg
    17821 gttttcaaac ggcattttat agcggattcg gataaaaaac cgcacccttt ccgccatttc
    17881 gggattttgc ccccgcaata cagaaaaccc cgaaaccgtc aggcttcagg gttttccgct
    17941 tatcgcgtat caaccgcctt ggcggttttg caaaaatcaa gccagtgctt ttacttttgc
    18001 agacagacgg cttttgtggc gtgccgcttt gtttttgtgg aacacgccct tgtcggcgat
    18061 gcggtcgatg actttgacag actcttggta aaccgcttgt gcggcagctt tatcgcctgc
    18121 ttcgactgct ttcaatactt ttttcactgc agtgcggaat gcggtacgca ggctagcgtt
    18181 gtgggcgcgt tgtttgacgg actggcgggc acgtttgcgt gcttgtgcgc tgtttgccat
    18241 attggaatct cctggaagaa aaataaattc tgtaaacgcg caattttaaa gggggtttac
    18301 attatatgca agccttttct ctctatccgc ccgtcggaca ccctgacagc gcaaaaaaac
    18361 ggcaaaatcg tgcggacgtt ttccccctct tttgaaaaca aggtaatttt ttaggaaaat
    18421 caggtcaata tcgctttgag tttgcacaaa tttactttac aatgcccaaa ccttgcgccg
    18481 gggtgtttat ggggcgtggg gttttctttt tttccaacct ctttcatcag gaatctgccc
    18541 gtatgaaaaa atccgtatta gccgttttgg ccgcgctgtc tctggcagcc tgcggcggca
    18601 gcgaaaaaaa cgccgtgcaa cctcaagcag acgctgcgtc tgctgccaat gccgaagccg
    18661 ctgccaccga tactctgaac atctacaact ggtcgaacta tgttgacgag agtacggtcg
    18721 aagactttaa aaaagccaac aatctgaagc tgacttacga cttgtacgaa aacaatgaaa
    18781 cgctggaagc caaaatgctg accggcaaat ccggttacga tttggttgtg ccgggcatcg
    18841 ccttcctgcc gcgccaaatc gaggcgggcg cgtatcaaaa agtcaacaaa gacctgattc
    18901 ccaactataa aaacatcgat cccgaactct tgaaaatgct ggaaaccgcc gacccgggca
    18961 accagtatgc cgtcccctat ttctccggcg tgaacacgat tgcgattacg gcgaaaggca
    19021 aagagctttt gggcggcaag ctgcccgaaa acggctggga tttgctgttc aaacccgaat
    19081 acacccacaa gctgaaatcc tgcggcatcg ccctgtggga caccccaagc gaaatgttcc
    19141 cgattttgct gaactacttg ggcaaagacc ccaaaggctc gaatcctgaa gatttgaagg
    19201 cagcggcgga agtgttgaag tctatccgtc cggatgtcaa acgtttcagc ccgtccatca
    19261 ttgacgaatt ggcgcgcggc gatatctgct tggcggcagg taacggcggc gatttgaact
    19321 tggcgaaagc acgttccgaa gaagtgaaaa acaacgtcgg catcgaagtg ctgaatccga
    19381 aaggcatggg cttctggatt gagtcttggc tgattcccgc cgatgcgaaa aacgtcgcca
    19441 atgcccacaa atacatcaac tacacgctcg accccgaaat cgcggcgaaa aacggcatcg
    19501 ccgtaacctt cgcccccgcc agcaaaccgg cgcgcgaaaa aatgcctgcc gaactggtaa
    19561 acacccgttc catcttcccg aacgagcagg atatgaaaga cggtttcgtg atgccgcaaa
    19621 tgagcacgga tgcgaaaaaa ctgtcggtca gcctgtggca gaaaatcaaa gtcggcacca
    19681 actgatttga agcattaaaa atgccgtccg aacatcgttc ggacggcatt ttatattgga
    19741 ttgaaataga aatatttata tagtggatta acaaaaatca ggacaaggcg acgaa
//
LOCUS       DAXHTR010000017        19626 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.017, whole genome shotgun sequence.
ACCESSION   DAXHTR010000017 DAXHTR010000000
VERSION     DAXHTR010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19626
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.017"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            168..791
                     /gene="lolA"
                     /locus_tag="ACFNML_000442"
     CDS             168..791
                     /gene="lolA"
                     /locus_tag="ACFNML_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFC6391880.1"
                     /translation="MMKPHNLFKFLAVCSLTVSVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            1064..2200
                     /locus_tag="ACFNML_000443"
     CDS             1064..2200
                     /locus_tag="ACFNML_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6391881.1"
                     /translation="MKKTLVAAILSLALTACGGGSDTAAQAPSAKPEAEQSGKLNIYN
                     WSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQIK
                     AGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTRQVQKALG
                     TDKLPENEWDLVFNPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKAA
                     VDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVLT
                     PKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLLPEVAAKNGSFVTYAPASRPARELMD
                     EKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            2332..3381
                     /locus_tag="ACFNML_000444"
     CDS             2332..3381
                     /locus_tag="ACFNML_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6391882.1"
                     /translation="MDLSIVVPIYNVESYLEACLNSIEPILSNENVELILVNDGSKDG
                     SEDICYKYIDKTTNNKQQTTNNKQQTTNNKQQTTNNTRYRTPNIKYIYQDNQGTAEAR
                     NTGIKNSNGKYIVFIDSDDFINCQVLLDFLSKDDIDMPDVVFLNAVKYDKGSVSHFGE
                     DYQPEKILKQSKVEVLKGLCRFRKFPGSACNKIIKRELIIKEKLFFEKEVYAEDIEWS
                     MRLFNAATTFSYLDGCYYYYRQGRKDSKTGTVSEKSIKSLLYILEKNAEMEFDRDISS
                     YLYSFLSYEYLVLLFIMTSKNIECDADIKRRAYHLRFMLLKSNKLIYKLIFPIITLFG
                     VDITGRILKAIRGNI"
     gene            3422..3952
                     /locus_tag="ACFNML_000445"
     CDS             3422..3952
                     /locus_tag="ACFNML_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFC6391883.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGSTVLDDAVIEDEVMIGAGSLVPPRKRLAGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(3986..4102)
                     /locus_tag="ACFNML_000446"
     CDS             complement(3986..4102)
                     /locus_tag="ACFNML_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HFC6391884.1"
                     /translation="MPSEPEKRLSDGIFACNSNADGQNQGASDTVIGCVPSA"
     gene            complement(4327..5922)
                     /locus_tag="ACFNML_000447"
     CDS             complement(4327..5922)
                     /locus_tag="ACFNML_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFC6391885.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMDIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRNTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIDNPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIDWTPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGRFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(6029..6424)
                     /gene="hisI"
                     /locus_tag="ACFNML_000448"
     CDS             complement(6029..6424)
                     /gene="hisI"
                     /locus_tag="ACFNML_000448"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HFC6391886.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGGAWETADAVLKDEKEIYGSTH"
     gene            complement(6455..7222)
                     /gene="hisF"
                     /locus_tag="ACFNML_000449"
     CDS             complement(6455..7222)
                     /gene="hisF"
                     /locus_tag="ACFNML_000449"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFC6391887.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGDPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAVNSENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGVFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(7235..7972)
                     /gene="hisA"
                     /locus_tag="ACFNML_000450"
     CDS             complement(7235..7972)
                     /gene="hisA"
                     /locus_tag="ACFNML_000450"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFC6391888.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVAKDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(8005..8643)
                     /gene="hisH"
                     /locus_tag="ACFNML_000451"
     CDS             complement(8005..8643)
                     /gene="hisH"
                     /locus_tag="ACFNML_000451"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFC6391889.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPERVSR
                     ADKVIFPGQGAMPDCMAALTRGGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFERDLRDPQGYRLKVPHMGWNTVRQTQNHPLFKDIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(8774..10276)
                     /gene="pta"
                     /locus_tag="ACFNML_000452"
     CDS             complement(8774..10276)
                     /gene="pta"
                     /locus_tag="ACFNML_000452"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFC6391890.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMSQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDIVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSPVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(10461..11519)
                     /gene="fbpC"
                     /locus_tag="ACFNML_000453"
     CDS             complement(10461..11519)
                     /gene="fbpC"
                     /locus_tag="ACFNML_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HFC6391891.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(11540..13057)
                     /gene="fbpB"
                     /locus_tag="ACFNML_000454"
     CDS             complement(11540..13057)
                     /gene="fbpB"
                     /locus_tag="ACFNML_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HFC6391892.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(13125..14120)
                     /gene="fbpA"
                     /locus_tag="ACFNML_000455"
     CDS             complement(13125..14120)
                     /gene="fbpA"
                     /locus_tag="ACFNML_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HFC6391893.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALITYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(14371..14841)
                     /locus_tag="ACFNML_000456"
     CDS             complement(14371..14841)
                     /locus_tag="ACFNML_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
                     /protein_id="HFC6391894.1"
                     /translation="MTDTDTQADRFEQMMWQAVDKLFEQHDGKLESMDGWEQELVLIW
                     RAEADIGNGGILQFVCNWSFPAAEKTCSVLKKIGAIHSAMLIHRAADALDKEIRHLQS
                     EGKNLKEMWDITKHLDNKTASLPNSLDEQYWQDPDKLYLPGWQYYSGNPVQTLF"
     gene            complement(14898..16274)
                     /gene="argH"
                     /locus_tag="ACFNML_000457"
     CDS             complement(14898..16274)
                     /gene="argH"
                     /locus_tag="ACFNML_000457"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFC6391895.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDEGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIRSLIQSLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSKLPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREMSA"
     gene            complement(16293..17162)
                     /gene="galU"
                     /locus_tag="ACFNML_000458"
     CDS             complement(16293..17162)
                     /gene="galU"
                     /locus_tag="ACFNML_000458"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6391896.1"
                     /translation="MKPIKKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRITGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(17190..17789)
                     /gene="rdgB"
                     /locus_tag="ACFNML_000459"
     CDS             complement(17190..17789)
                     /gene="rdgB"
                     /locus_tag="ACFNML_000459"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035870 - dITP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0036222 - XTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HFC6391897.1"
                     /translation="MSEKPEKIVLASGNAGKLEEFGNLFKPYSITVLPQSAFGIPECP
                     EPYPTFVENALAKARHAAEYSGLPALADDSGICAAALNGAPGIHSARYAGDNPKSDTA
                     NNLKLAAELAGKTDKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHREQALADLIRKLAL"
     gene            complement(17782..18015)
                     /locus_tag="ACFNML_000460"
     CDS             complement(17782..18015)
                     /locus_tag="ACFNML_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NGO_0222 family membrane protein"
                     /protein_id="HFC6391898.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(18123..18656)
                     /locus_tag="ACFNML_000461"
     CDS             complement(18123..18656)
                     /locus_tag="ACFNML_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFC6391899.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(18758..19216)
                     /gene="nudB"
                     /locus_tag="ACFNML_000462"
     CDS             complement(18758..19216)
                     /gene="nudB"
                     /locus_tag="ACFNML_000462"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFC6391900.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFGLEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            19427..19503
                     /locus_tag="ACFNML_000463"
     tRNA            19427..19503
                     /locus_tag="ACFNML_000463"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19461..19463,aa:Pro,seq:ggg)
BASE COUNT         4221 a         4865 c         5825 g         4715 t
ORIGIN      
        1 ggcacacatt aaagggcagc gtggcgcgcc tgccttttcc gagcggtcaa aaaaatcagc
       61 cctcggaaaa cgcggtttgt aaaatgcaaa ccgcccgtaa cgccgcccgt atgattgttt
      121 tgccgcgccg atactttacg ccacacccat cccaacaagg aaaaataatg atgaaaccgc
      181 acaacctgtt caaattcctc gccgtttgct ccctgaccgt ctccgtcgct tccgcacagg
      241 cgggcgcggt ggacgcgctc aagcaattca acaacgatgc cgacggtatc agcggcagct
      301 tcacccaaac cgtccaaagc aaaaagaaaa cccaaaccgc gcacggcacg tttaaaatcc
      361 tgcgcccggg cctcttcaaa tgggaataca cttcgcccta caaacagact attgtcggcg
      421 acggtcaaac cgtttggctc tacgatgtcg atttggcaca agtgaccaag tcgtcccaag
      481 accaggccat aggcggcagc cccgccgcca tcctgtcgaa caaaaccgcc ctcgaaagca
      541 gctacacgct gaaagaggac ggttcgtcca acggcatcga ttatgtgctg gcaacgccca
      601 aacgcaacaa cgccggctac caatacatcc gcatcggctt caaaggcggc aacctcgccg
      661 ccatgcagct taaagacagc ttcggcaacc aaacctccat cagtttcggc ggtttgaata
      721 ccaatcccca actctcgcgc ggcgcgttca agtttacccc gcccaaaggc gtggacgtgt
      781 tgagcaactg atgccgtccg ccccgatgcc gtctgaaagc cgccgaggct tcagacggca
      841 ttttcacgca ggcggaacaa tgtcccgcat taccgcccga tcgggcatcg gaacggcaaa
      901 cccgtgaaaa ttaacggttg cgccccggtt gtttttgccg tttaatgcaa accttgctgc
      961 accaagggcc aagaaagccg accggccgcc cccacagctt ccgatgcagg cggccaggcc
     1021 gtccctgcaa tgttttttat ttttgaacga aaggtcgaaa accatgaaaa aaacactggt
     1081 ggcggcaatc ctgagcctcg ccttgactgc gtgcggcggc ggaagcgata ccgccgccca
     1141 agccccctcc gccaagcccg aagccgaaca atcgggcaaa ctcaatatct acaactggtc
     1201 ggattatgtc gatcccgaaa ccgtcgccgc ctttgaaaaa gaaaccggca tcaagacgcg
     1261 ttccgattat tacgacagca acgaaacact ggaggcaaaa gtcctgaccg gcaaatccgg
     1321 ctacgacctg accgcgccgt ccatcgccaa cgtcggccgg caaatcaaag cgggcgcgta
     1381 tcagaaaatc gacaaggcgc aaatccccca ttacggcaac atcgataaag atttgctgaa
     1441 aatgatggaa gccgtcgatc cgggcaacga atacgccgtc ccctatttct ggggcatcaa
     1501 caccttggca atcaacacgc ggcaggtgca aaaggcattg ggtacggaca agctgcccga
     1561 aaacgaatgg gatttggtgt tcaaccccga atacaccgcc aaactcaaat cctgcggcat
     1621 cagctatttc gacagcgcaa tcgaacagat tcccttggcg ttgcactatt tgggcaaaga
     1681 ccccaacagt gaaaatcccg aagacatcaa agccgccgtc gatatgatga aagccgtccg
     1741 aggcgacgtg aaacgcttca gctcttccgg ctatatcgac gatatggcgg cgggcaacct
     1801 gtgtgccgcc atcggttacg gcggcgattt gaacattgcc aaaacccgtg ccgaagaagc
     1861 cgcaaacggc gtggaaatca aagtattgac cccgaaaacc ggcgtgggcg tgtgggtgga
     1921 ttcctttatg attccgcgcg acgcgcaaaa cgttgccaac gcccaccgct atatcgacta
     1981 cacgctcctg cccgaggtgg cggcgaaaaa cggcagcttc gttacctacg cacccgccag
     2041 ccgccccgcg cgcgagctga tggatgaaaa atacacctcc gatgcatcga ttttcccgaa
     2101 caaagaactg atggaaaaaa gtttcatcgt atcgcccaaa tccgcagaat ccgccaaact
     2161 gggcgtgaag ctgtggcaag ggctcaaagc gggcaaataa ccggaatccc tgccgtctga
     2221 aacctttcgg gcggcaggaa acggcgcgtc cgttatcaaa cagggggcgt ttccccgccc
     2281 gcctgttatg attgggttaa gattaaaatg atttagtaaa atgagaaaga tatggattta
     2341 agtatcgtag ttcccattta taatgtcgag agttatttgg aagcgtgtct aaattccata
     2401 gaacctatat taagtaatga aaatgtcgaa cttatccttg tgaatgacgg gtcaaaagac
     2461 ggaagtgaag atatatgtta taaatatata gataaaacaa caaacaacaa acaacaaaca
     2521 acaaacaaca aacaacaaac aacaaacaac aaacaacaaa caacaaacaa caccagatac
     2581 cgaacaccaa acatcaaata tatatatcag gataaccaag ggacggcaga agctaggaat
     2641 accggaataa aaaattcaaa cggaaaatat atagtcttta ttgattcgga tgattttatt
     2701 aattgtcagg tcttgctgga ttttcttagt aaagatgata ttgatatgcc ggatgtggtt
     2761 tttttaaatg cggttaaata tgataaggga agtgtttcac attttggcga agattatcag
     2821 cctgaaaaaa tactcaagca atccaaagtc gaagttttga aaggattatg ccgatttaga
     2881 aaatttccgg gttcggcgtg taataagatt ataaaaaggg aattgattat caaagaaaaa
     2941 ctgttttttg aaaaagaggt ttatgccgaa gatatcgaat ggtcaatgag attatttaat
     3001 gcggcaacaa ctttttctta tttggacggt tgttattact attatcggca gggaagaaaa
     3061 gattctaaaa caggaactgt ttcggaaaaa agtataaagt cattattata tattttggag
     3121 aaaaatgcgg aaatggaatt tgatagggat atatcaagtt atctttattc ttttctttcc
     3181 tacgaatatc tcgttttgct ttttatcatg acgagtaaaa atatagagtg tgatgctgat
     3241 ataaaaagaa gggcgtatca tttaaggttt atgctgttaa agtccaataa attgatatat
     3301 aagctgatat tcccgataat cacattattc ggggtcgata ttacaggcag gattttaaaa
     3361 gcaatcaggg ggaatattta ataaatcctt taacactata catcttaccg aaggaggaaa
     3421 aatgaacgca atccgaactt tccaaaaccg cacgcccgaa atccacgaaa gctgtatgat
     3481 agacgaagcg tgcgtcgtca tcggcgaagt gtcgcttgcc gaagacgttt ctgtgtggcc
     3541 gtgcgccgtg ttgcgcggcg atgtgaacag catcaccgtc ggcgcgcgca gcaatataca
     3601 ggacggcagc gtcctgcacg tttcccacaa aaccgccgcc aaacccgaag ggtcgccgct
     3661 ggttatcggc gaagacgtta ccgtggggca caaagtgatg ctgcacggct gccgtatcgg
     3721 caaccgcgtc ctggtcggta tgggcagcac cgttttggac gatgccgtga ttgaggacga
     3781 agtgatgatc ggcgcgggca gcctcgtgcc tccgcgcaaa cgtttggcgg gcggctacct
     3841 ttatgtcggt tcgccggtca gacaagtgcg cgtgctgacc gatgaggaaa aagccttttt
     3901 gaaatattcc gccgcgcatt atgtgaagct gtcgaaacag tacgggatgt gaaatcacat
     3961 cggcgttctt gcgtcagccc caaattcatg cggatgggac gcatccgata acggtatccg
     4021 atgcgccttg attttgaccg tctgcgtttg agttgcaggc aaaaatgccg tctgaaagcc
     4081 ttttttcggg ttcagacggc attttatcgc cgattgcttt ttacagtttg accgaatgtt
     4141 cgcgcgtttc gtggaacacg atgtccggcc aacgttcttg cgtcagccct aaattcatgc
     4201 ggacgggatg cccgataacg gtatccgatg cgccttgatt ttgatcggtg catttgagtt
     4261 gcaggcaaaa atgccgtctg aagccctttt ttcgggttca gacggcattt tattgccgat
     4321 tgctttttaa agtttgaccg aatgctcgcg cgtttcgtgg aacacgatgt ccggccagcg
     4381 ttcttgcgtg agtcccagat tcacgcggtt aggggcgagg taggcgaggt tgccgcccgc
     4441 gtcgatggcg aggttgcctg cattggcttt ttcaaattcc gccagttttt tcttgtcgtc
     4501 gcacgatacc cagcgcgccg accagatgga tgcgctgtcg aacacggctt ctacgccgta
     4561 ttcgttggcc agccgcgagg taacgacttc aaattgcaac acgccgaccg cgcccaaaat
     4621 caaatccgcg ccgctcatcg gtttgaacac ctgcaccgca ccttcttcgc cgagttgttg
     4681 caagcctttt tgcagttgct tgattttcag cgggtttttg atgcggacgc tgcggaacag
     4741 ttcgggcgcg aagaatggga tgccggtgaa cgccagttgt tcgccttcgg agaagctgtc
     4801 gccgatttgg atgttgccgt ggttcgggat gccgatgatg tcgccggcgt aggcttcttc
     4861 caccagctcg cggtcgtgcg acatgaaggt taccacgctg gaggcggcga tttcgcggtt
     4921 gatgcgaagg tgtttcatct tcataccgcg ctcgaatctg ccggagcaga cgcgcaggaa
     4981 ggcgatgcgg tcgcggtgtt tcggatccat attggcttgg attttaaaga taaatccgga
     5041 gaatttcggc tcgtccggct cgaccatacg tacggtcgcg tcgcgcggtt tcggcgcggg
     5101 cgtccagtca atcaatgaat tgaggatttc ctgaataccg aagttgttaa tcgcagagcc
     5161 gaagaatacg ggcgtgagtt cgccggcgag gaattcgtcg agattaaact cgttggaagc
     5221 cgcctgcacc aattcgattt cgtcgcgcaa ctgctggatt tccaatggaa agcgttgttc
     5281 caattcggga ttgtcgatgc ctttgatgat gtcgaactcg tgcggcagac gttcgccacc
     5341 ggcttcaaag agatagattt cgtcgttcag gatgtggtac acgcctttga agtttttgcc
     5401 catgccgatc ggccaggtta cgggcgcgca gcggatttgc aggatgtttt ccacttcgtc
     5461 cagcaactcc aaagaatcgc gcacttcgcg gtcgtatttg ttcatgaacg tgacaatcgg
     5521 cgtattgcgc aggcggcaga cgttcaagag tttgatggtt tgcgcttcca caccttttgc
     5581 cgcgtcgatg accatcaacg cgctgtccac ggcggtcaga acgcggtagg tgtcttcgga
     5641 gaagtcttgg tgtcccggcg tgtccaagag gttgacggtg tggtctttgt agtcgaactg
     5701 catcacgctt gatgccacgg aaatgccgcg ctgcttctcg atgtccatcc agtcggaggt
     5761 ggcgaatttg ccggtttttt tgccttttac cgtacccgcg ctctgaatcg cgcccgaaaa
     5821 cagcaacagc ttttcggtca gcgtggtttt acccgcgtcg gggtgggaga tgatggcaaa
     5881 cgtgcggcgg cggcgcactt ggtcgaggat ttcttgggac atggttttct ttgcaaaaag
     5941 gttcaggccg cttttcagac ggcccggaca gtgtttgaga cggcgaaatt gtacaaaaaa
     6001 atgcctgata attcaatgtt ggaggcggtc agtgcgtgct gccgtaaatc tctttttcgt
     6061 ctttcaggac ggcatcggcg gtttcccacg cgccgccgcg ccagactttg taaaagcagc
     6121 tttctcgccc ggtgtggcag gcgatgccgc cgttttgggc gatgagcatc acaatggcgt
     6181 cgccgtcgca gtcgaggcgc agttcgcgga ctttttgcgt gtgtcccgac tcttcgccct
     6241 tcatccattg tttttggcgc gaacggctgt aatagtgggc aaagccggtt tcgacggttt
     6301 tttgcagggc ttcggcgttc atccacgcta ccattaaaac acgtttggtt tcggcatctt
     6361 gggcgatggc gcaaaccaaa cctttttcgt caaatttgac ggcttcaagc aggtttttat
     6421 ccatatttcc tttcagacgg catggtcggg gcggtcagag gcgcacttcg atgccggctt
     6481 cgcgcatagc gcgtttggct tcgcggatgg cgatttcccc gaaatggaaa acgccggcgg
     6541 caagtacggc atcggctttg ccttcggtta tgccttcaat caggtgccgg acattgccga
     6601 cccctccgga ggcgatgacg gggatgtcga cggcttcggc aacggcgcgg gtcagcggca
     6661 ggttgaaacc ctgtttcgta ccgtccctgt ccatcccggt gagcaggatt tcgcccgcgc
     6721 cgcgtttttg catttcgatc gcccattcca ccgcatccaa accggtcgga ttgcgcccgc
     6781 cgtgggtaaa gatttcccag cgtgtgtttt cggagttgac ggctttggca tcgacggcgg
     6841 cgacgatggc ttgcgaaccg aaaaatccgg cggcttcgtc aatcaggtcg ggacgggtaa
     6901 cggcggcggt gttgatgctg actttgtccg cgccggcatt gagcaggcgg cggatgtcgg
     6961 caacggtgcg tacgccgccg ccgacggtca gggggatgaa gacttgtccg gcaacctctt
     7021 cgatgatgtg caggatggtg tcgcggttgt cggatgaggc ggtgatgtcg aggaaggtca
     7081 attcgtccgc gccttcgccg ttgtagcgtt tggcggcttc gacggggtcg cccgcgtcgc
     7141 gcaaaccgat gaagttcacg cctttgacga cgcgcccgtc ttttacgtcg agacagggga
     7201 tgatgcgttt tgccagtgcc ataatcggat gcctttagtc gagggaatct gccagttgct
     7261 gcgcttgggc aaaatcgatg ctgccctcgt aaatcgcgcg gccggtaatc gcgcctgcta
     7321 cgccatgttt ttcggcggca cacagggcgc ggatgtcgtc caagccggtc agtccgccgg
     7381 aggcgatgac gggaatgcgg acggcttggg cgagtttgac cgtcgcgtcg atgttcacgc
     7441 cgctcatcat accgtcgcgc ccgatgtcgg tgtagatgat gctgttgacg ccgtcgtctt
     7501 caaagcgttt tgccaaatca attacatgat gcccggttac ggttgcccag ccgtcgatgg
     7561 cggccatacc gtctttggca tccagcccga caataatcct gccggggaag gctttgcacg
     7621 cctcgcgcac gaagtcgggg tttttgaccg ccgccgtgcc gataatcacg tcgtttaagc
     7681 ccaaatccaa atattgtccg atggttttca aatcgcgtat gccgccgccg agctgtacgg
     7741 ggatgtcttt ggcgacagcg gcaaggatgt ctttgatggc gggcaggttt tgcggaacgc
     7801 cggcaaacgc gccgttcaaa tctaccagat gcaggcggcg cgcgccttgt ttgaaccagt
     7861 gcagcgcggt ttcggcgggc gaatcggaaa agacggtcgc ctcttccatc agcccttgtt
     7921 tcaggcggac gcagcgtcct tctttcaaat cgatggcggg tatcagcagc ataatttttc
     7981 tccttgtgcg gggccgtgtc cggcttacca gtttaaaaag tttttcaaca tcgtcagccc
     8041 ggcatcgtgg cttttttcgg tgtgaaattg cgtggcgaat acgttgtctt tgccgacgat
     8101 gcaggcaaac ggggacgggt agtcgctttc gcccaatatg gtttcgggat tttcgggggc
     8161 gaaatagtag ctgtggacga agtaaaaacg cgtgtcttgg ggaatatctt taaacagcgg
     8221 gtggttttgg gtttggcgca cggtgttcca gcccatatgc gggactttca gacggtatcc
     8281 ctgcgggtcg cggaggtcgc gctcaaagcg tctgactttg ccgccgaacc agcccaagcc
     8341 gtcggtgttt ccttcttcac tgtggtcgaa taaaagttgc gcgccgacgc agattccgaa
     8401 aaagggtttg ttttttaagg catctttgac tgcctcgtcc aggccgccgc gcgtcagtgc
     8461 cgccatacag tcgggcatcg cgccctgccc gggaaaaatg actttgtcag cgcgggacac
     8521 gcgttcgggg tcgccgctta aaaagatttc ggtatttttt ccggcaagct gccccgccgt
     8581 ccggacggat ttcaatacgg aatgcaggtt gcccataccg taatcgataa tggcgatttg
     8641 catggcttcc tcctcttttt ttgcaatatg gctgcgattt taacaaacaa atgtgccgtg
     8701 ctgataaaaa tgccgtctga aaacgggagt ctgtcttcag acggcatagg gttcaaaccc
     8761 ggaaagccgt ttgtcagcct tccatttgtt ttgcctgaac ggcagtcagg gcgatggtga
     8821 acacgatgtc ttccaccagc gcgccgcgcg acaggtcgtt gaccggtttg cgcaggcctt
     8881 gcagcagcgg gccgacgctt aagacgttgg cactgcgttg gacggcttta taggtgcagt
     8941 tgccggtgtt caggtcgggg aagaccaaaa cggttgcctg tcctgccacc gggctgcccg
     9001 gagctttgga tttgcccacg cccggcacgg ttgccgcatc atattgcagc gggccgtcga
     9061 tggcgaggtc ggggcgtttt tcccgggcaa gtttggttgc ttcgatgacg atatcgacat
     9121 cggggccgct gccggagttg acggtggagt aggaaatcat cgccactttc gggtcgatgc
     9181 cgaaggcttt tgcggaatcg gcagactgga tggcgatgtc ggcaagctgt tgcgcggtcg
     9241 ggttcggatt aaccgcgcag tcgccgaaga cgaggacttg gttgggcagc agcataaaga
     9301 atacgctgga cacgaggctt gcgcccggtg cggttttaat cagttgcaaa gcggggcgga
     9361 tggtgttggc ggtggtgtga accgcaccgg ataccaaacc gtccacatca ttttgcgaca
     9421 tcatcatcgt accgagtacc acggtgtctt gcagttgctt gcgcgcgtct tcgggtgtca
     9481 ggcctttgga tttgcgcagt tcgcacatcg gctcgacgta ttgttcgacc aatgaggcgg
     9541 gatcgatgat ttccaaagag tcgggcaggc tgatgccgcg ttctttggca acggcttcga
     9601 cttcttcgcg tttggcaagc aggacgcagc gggcaatgcc tttttcgtgg cagatggcgg
     9661 cggcttggac ggtgcggggt tctgcgccct caggcaggac gatgcgtttg tcggcttggc
     9721 gggcgaagtc gatcaggttg tagcggaatt gcgccggcga caggcgtttt gcttcgcggc
     9781 ctgccaatac ggatacgtct ttcagcgcgt cgctcgaacc gaagaaggtc aggccggttt
     9841 tttcggctgc cgcttcggca acggaggctg ccgcgccgtc cacgacaaaa ccttccaata
     9901 cgcccggcgc ggcggcgaag aactgtttgg caaggttcag ccgatttgcc agttcgtcgg
     9961 catcggcgtt gtcggaacgg acggcgaaga cggctgccgc gtcaagggac aatgccagtt
    10021 cgacgttttt gcctgcgagg tagattttgt cggcatcggg cgcgatgcct tcgatgacga
    10081 ggttggcggc atcgagtgcg gcaactttgc cgaccagtgc gtcgaaccag tcgtcgcttt
    10141 tgccttgcgc gagcagggtt tcggcggttg cgtcaacggc ttggaaaatt tgtgcgtcca
    10201 gtgcttttgc aaaggcttgt gcggcggcgg aggcgtccag tccggcagat acgggtacga
    10261 tgagtacttt tgccatgata tatcctttcg tatgctgcgg tgtgcggcat atgtggttgg
    10321 aaggggcggc atataggcag aaacggctgc ctgcgtgccg tgcgtgccgt gtttggcttg
    10381 aggcgcgcag gttgaatata gcaaacaaat tctgtttcca acaagataaa tatccgcagg
    10441 cttgtggatg ctgccgcctt tcagagggta tttccgggga agaacagggc gggaccgtcc
    10501 aaatggagga cggcggaaat gccgtctgac agggtggggg cggaagggag gttgagcgtg
    10561 aggacggttt gtccgacccg gagactgatt tcggtatgcc gcgctttggg cgtggttttg
    10621 agaaccacgg cgtgaatgga ggcggtgggt gcggaatggg ggtgaaggct gaactgttcc
    10681 ggacggatga gcagtgtgcc gcgtgtgcct gcgggtgcgc cgctttgaac gggcaggcgg
    10741 cccaatccgc aatcggcggt gccgtcggcg ttgagcgcgg cggggaacac gatgccttcg
    10801 ccgataaaca gggcggcatc aaggtcggca ggttgtcggt acaattcgtg agggcttgca
    10861 gtttggagga tgcgcccctg tttcatcacg gcaatccggt cggcgtattg cagggcttct
    10921 tcgcggtcgt ggctgacgaa aacggcagat ttgccgttgg cgcgcagggc ggcaatcatg
    10981 tcttcgcgaa tctggcggcg caactgttcg tccagcgcgc tgaagggttc gtccaacaaa
    11041 atcagttcgg gatcgggtgc gagggcgcgg gcgagggcga cgcgctgttg ctgtccgccc
    11101 gaaagttcgt gcggatagcg tccggcaagt tcggaaatgc cggtcaattc caacatagct
    11161 tcgatgcgct gccgctcttg cgccgtcttg cctttgccgt tgcccagccc gtaggcggtg
    11221 ttgcggtaaa cggtcaggtg ggggaacagc acaccttcct gtacgacata acccaaacgg
    11281 cgttcgcgga cggggaggtt ggtatttttc gagaagatgg ttctgccgga aagcgaaatt
    11341 tcgccaaaat cgggttgttc aaaaccggca aggcagcgta aaagggtggt tttgccgcag
    11401 ccggacgcgc cgacgataaa gaggatttcg cccgggtcga ggctgagcga aatgtcgttt
    11461 aaaactgggg tgttttgaaa acttttggac aggtgtccga tgtgcagggc ggcggtcatg
    11521 gcggtacttc ctcaagctgt tatttgaagg cgtatttctt cagcaggaat acggggatgc
    11581 cggaaaataa taccagcatc agcgcgtaag gggtggcggc ggcgtattgt gcgtccgatg
    11641 tgtattccca aacggcggtg gagagtgtgt ggacatcgtc ggtggtcagc agcagggtgg
    11701 cggtcagctc tttcatcagt ttgaggaaga cgagtgcgaa tgcggcggta atgccgggca
    11761 ggatggacgg cagtaccaac gtcctgaaaa taaagaagtg tccgcgcccc aatgttgcgc
    11821 cgacctgttc catccctttt gggagttgtt ccaaggaagt cctcagggtg gtttgcgcca
    11881 tcggcaggta aagcatgaaa taggcaagga tgacgacgat aaaggtttgg taaacggcag
    11941 gggtgtagtt gatgctgaaa taaaccaagg atagggcgat aaccaaaccg gggacggcgt
    12001 gcagtaaaaa cggcagcctg tctatccaaa cggttaaaaa attgcgatag cgaaccgatg
    12061 cccaaacaag gggcaaggca cataatatag tcaaaatcgc acctaaagcc gatacgctta
    12121 aggaacggat aaaggcatca aatacggata cgagcgcgaa tgtgccggaa gtgccgacca
    12181 tcatccaatg tatcaatacg ccaaagggga taataatgcc caaagtcaac aagctgctta
    12241 aaaaaacaat cgcgccaatc tgaccgggca gtttgagggt tttgacggga taaggacggg
    12301 caacgccttt gccgctgtgg taaatcttgg ctttgccgcg aaatatgctt tctccaaata
    12361 cgacgatgcc gcacaccgcc attaaaacag cggaaagcag ggcggcggta ttgttgttgt
    12421 aggacatttc gtattcttgg aaaatggcgg tggtaaaagt ggggtagttc aaaatggata
    12481 ccgcgccaaa ttcgaccagc atatgcaggg caatcagtaa cacgctgctg ccgatggcgg
    12541 gtttgagctg ggggaggatg gcggaaaaaa aggtttgcag gcggcttttg cccaaggaca
    12601 ggctgacttc ttcgtaagac aggctgatgc gtttgagtgc cgcctcgacg ggcaggtagg
    12661 cgagcgggaa cgaggacagg ctcataatca tcactgtccc ccaaaagcct tcgacacgga
    12721 aggtcaggct gatccaggtg aaacagctga caaatgcggg gatgcacaaa ggcagggtga
    12781 ttgccgtctg aaaaaaggtt ttgccgaaga agcggtaacg ttggaacaaa agggcgcagg
    12841 caatgcccaa aacaatggaa atcagggtaa cgcccgccat catcgtcaag gtgttggaga
    12901 gcaaatccca catacgcggg cggaacaaca gttcgacggc gcggttgatg ccgacctgcc
    12961 acgaacgcat agcgacatat aaaaaaggca gggtaagcgg tagggcaatc agtaggatga
    13021 ggccggtaag ccaaatgggt atttttttag gagacatagt gtttttcatc ggcaaaacgg
    13081 gcggacagta taaatgtcca cccgtttgac aatccgaaaa cggcttattt cataccggct
    13141 tgctcaagca gccgggtggc gtgttctttt tcggaaacag tggtggcgga cacttggggt
    13201 gcttccaact tggcgatggg ttccaaattg aaggtggata ccacgtgcgg attcaaagga
    13261 tattcggcac ggacggcggt cagggcgcgc tgtccttcct tgctggcgag gaaggcgacg
    13321 aattttttcg cctcatcctt gttttgggag gattttaaca cggctgcgcc ggaataggta
    13381 atgagtgcgc cgggatctct gtggcggacg aaattcaggc gggtgtggac attttgtacg
    13441 cctttttcac gcgcgaaagc gtgccagtag tagttgttga tgagggcggc atcgatttcg
    13501 ccgttttcaa ccgcttgaag ggcgacggag tttttagcgt aaggcttgcc gtattctttc
    13561 agacctttga gccatttcaa tgcggccgct tcgcctttca gtttgacgat ggcgacaacc
    13621 tgttccaaga acgcgccgga agtgggggcg taaccgatgc ggtttttcca tttcggcgtg
    13681 gcgtaattca ggacggattt ttccaaatct ttttcagaca gtttgcgggt gtcgtaaacg
    13741 acgacgcgcg aacgtccgct cagtgccacc cagtcttttt tggcggcaac cggcacgccc
    13801 ttgccgcgtg tttcgttgat ggtggaggcg ggcaggggct ctaggaggtt ggctgcggaa
    13861 agggtggcga gtgccgggat ttgttcggaa tagaatacgt cggcggggct tcggctgcct
    13921 tcttctttga tttgaccggc aagctggtcg cctttggcgc tgttgagttt gactttgatg
    13981 ccggtagccc gggtaaaggc atctgcaacg gcttgtgccg cttctttgtg ttggccgttg
    14041 tacacggtaa tgtctgccag cgcgggggtt gcggcggtca gggctgcggc aagcagtgcg
    14101 tatcggatag atgttttcat atcgattttc tcctaatgaa tgagagtgta caccttgtta
    14161 agacataacg gcgtgtagtg tattccttct tctttataaa tgcaaataat tattttttaa
    14221 atttgttatt atccgatccg gttatggttt gttctgactt gtattttttc cgtgcatcgc
    14281 gcccgtaagg cggaagcggc gggcaatgcg tggcggaatg tgggtaaagg cggcattttg
    14341 atttgtcgga atgcttgaga acccctctct ttaaaacagc gtctgaacag ggttgccgga
    14401 atagtattgc catcccggca gatacagttt gtcggggtct tgccaatatt gttcatccag
    14461 actgttcggc agtgaggcgg ttttgttgtc gagatgtttt gttatatccc acatttcttt
    14521 caggttttta ccttccgatt ggaggtggcg gatttctttg tccaatgcgt ctgccgcccg
    14581 atggatcagc attgcgctgt gtatcgcgcc gatttttttc agaacggaac aagtcttttc
    14641 ggcggcgggg aaactccagt tgcagacaaa ttgcagtatg ccgccgttgc cgatatcggc
    14701 ttctgcccgc caaattaaaa ccagctcctg ttcccacccg tccatgcttt cgagtttgcc
    14761 gtcatgctgt tcaaaaagtt tgtccaccgc ctgccacatc atctgttcga agcggtcggc
    14821 ttgggtatcc gtatcggtca tagtgttctt gtcttttaaa aatgccgtct gaacatttct
    14881 tcagacggca tcgggggtta agctgacatt tcccgccagc gtttgacttg gaaacggact
    14941 tgttcaggcg cggtaccgcc caagtggttg cgcgcgttta agctgccttc gggtgtcagc
    15001 acgccgtaaa cgtcgtcggc aatcaaatcg ctgaaacctt gtaagacttc gagcggcagt
    15061 ttgctcaaat cgacacccgc ttggtcggcg tggcgcacgg cttgggcgac gacttcgtgg
    15121 gcatcgcgga aaggcatgcc tttttttacc aagtaatctg ccaaatcggt ggcggtagcg
    15181 aagccctgca tcacggcggc gcgcatattg tcgggtttga cggttacgcc gcgcatcata
    15241 tcggcgtaaa tccgcaacgt gtcgataagc gtgtcggcgg tgtcgaacaa gggttctttg
    15301 tcttcctgat tgtctttgtt gtacgccaag ggctgcgatt tcatcagggt aatcaagccg
    15361 ataaggtgtc cgatgactcg gccggatttg ccgcgcacga gttcgggcac gtcgggattt
    15421 ttcttctgcg gcatgatgga cgaacctgtg cagaaacggt cggcgatgtc gataaagccg
    15481 aaacgcggac tcatccacaa aatcaattct tcagacaggc ggctcaggtg aaccataacc
    15541 agcgaggcgg cagccgtgaa ctcgacggca aaatcgcggt cggatacggc atcgagcgag
    15601 ttttggcaga tttgctcgaa acccagtaat tcggcggtaa tttcgcgccg aatcgggtag
    15661 gtcgtaccgg caagggcggc tgcgccgagc ggcatacggt tgacgcggcg gcggcagtcc
    15721 gccatccgtt cgttatcgcg gccgagcatt tcgacgtagg cgagcatatg gtgtccgaag
    15781 ctgacgggct gggcgacttg caggtgggta aagcctggca tgacggtttc ggcgttttgt
    15841 tccgccaaat ccagcaatgc cgtctgaagg ctttgaatca ggcttcggat gacggtaatc
    15901 tggtcgcgca gccaaaggcg gatgtcggtg gcgacttggt cgttgcggct gcggccggtg
    15961 tgcaggcgtt tgcccgcgtc gccgattttg tcggtcaggc ggcgttcgat gttcatgtgg
    16021 acatcttcca aatcggacga ccattcgatt ttgccgctgc ggatttcttc gaggatttcc
    16081 gccatgcccc gacggatgtc cgccaaatcg ccttcgtcca acacgccggt ttctttcagc
    16141 atttgcgcgt gggcaagcga gccttggatg tcccattcgg caaggcgtcg gtcgaaaccg
    16201 atggaggcgg tgtattgttt gacgagttcg gaaacgggtt cgttgaaacg tccggaccag
    16261 gttttgtcgt gcataaggat tccttgatgt ggttattcgg tacggtattt ttccaaaagg
    16321 cggcggaagg gttcgccggt ttcggggtgt ttcaggccgt aggcgacggt ggcttccaga
    16381 tagcccagtt tgctgccgca gtcgtagcgc gtgccttcaa aggggtgcgc taggacaaat
    16441 tcgtgatcga gcagcttggc gatgccgtct gtaagctgga tttcgttgcc cgcgccgcgc
    16501 ggcagtccgg tcagtaagtc gaaaatgcgc ggggtgagga tgtagcgtcc aacaacggca
    16561 aggttggagg gcgcgtcttc gggcttgggt ttttcgacaa tgccggtaat gcgttggaac
    16621 tgtttgagct gttcggtttc gacgatgccg tatgagccgg tttgcgatgc ttcaacggtt
    16681 tctacgccca aaatgctgtt gccgctgcgc ccgtacactt cgaccatttg tttgagcgcg
    16741 cctttggggg catcaatcag gtcgtcggca aggataacgg caaagggttc gtctccgatg
    16801 gcggcgcggg cgcacaagac ggcgtgtccc aagcccagtg cttccgcctg acggatgtag
    16861 aggcaggtaa tgttcggcgg caggatgttg cggacgtgtt ccaacaattt gtctttatgg
    16921 cgcatttcca actcggtttc gagttcgtat gccttgtcga aatggtcttc gatgctgcgt
    16981 ttgttgcgtc cggtaacaaa caccatttcc gtgcagccgg cttccacggc ttcttctacg
    17041 gcgtattgga tcagcggctt gtcgacgatg ggcagcattt ctttcgggct tgccttggtg
    17101 gcgggcagga agcgtgttcc catccctgcg acggggaaga cggctttttt tatcggtttc
    17161 attctttttc ctttgtattg ttttgatgtt taaagggcga gtttgcggat taaatcggcg
    17221 agtgcctgct cgcggtggct ttcgcggttt ttgacctccg aatccaattc ggcggcggtt
    17281 ttgccgtgtc cgggcagata aaaatacggg tcgtaaccga aaccgttttc accggcggga
    17341 acgccgttcc attgtccgtg ccatacgccc tcggcgataa ttggacgcgg gtcgtcttta
    17401 tggcggacaa aaaccaatac gcagacgtag cagcagcttt tgtcggtctt gccggcaagt
    17461 tcggcggcaa gtttcaggtt gttggcggta tcggatttgg gattgtcgcc cgcgtaacgt
    17521 gcggaatgga tgcccggcgc gccgtttagg gcggcggcac agatgccgct gtcgtcggca
    17581 agcgcgggca gcccgctgta ttcggcggca tggcgtgctt ttgccagcgc gttttcgaca
    17641 aaggtgggat agggttcggg gcattcgggt atgccgaatg cggattgcgg caatacggtg
    17701 atgctgtaag gtttgaataa gttgccgaat tcttcgagct tgccggcatt gccgcttgcc
    17761 aaaacgattt tttccggttt ttcagacata gcggttttcc tttgcggcgg attgggcggc
    17821 gcgtagggat ttgtgccgca ggtagagggc gaggctgccg atttgtccga acagtgcgcc
    17881 gaatgcgaaa agaaaggagg cgacggcgaa ggctttgctg ccgacggtca gcagataagc
    17941 acccaagagg acgagcagca taaatgccag tgtgaagagg gcaacagaca acagatagat
    18001 ttttcggcgg ttcatggcgt tcggtcggaa acggtatgtt cggattatag ccgattggga
    18061 cggtattccc tagagcttgg aaaaatgccg tctgaaacgg cgttcagacg gcatggcggg
    18121 cgttatgcct gtttgttcca acgttcgatg gattctttga tgactttttt cgcttcttcc
    18181 gcatcgcccc acgattcgac tttggtcgta cctgcttttt tcaggtcttt gtaatggttg
    18241 aagtggaact cgatttgttt gatcagctgt tgcggcaaat cggacaaagt tttgtaggcg
    18301 ttgccgttgt tgcggtcgtc ggcaggaacg cagacgattt tgtcgtccac ttcgccgtcg
    18361 tcaacgaatt tcatcacgcc gataacgcgc gcttccaaga atacgccggt tgccaaaggt
    18421 tgttcggtaa cgagcagcac gtccaattcg tcgccgtctt cgtccaaagt ttggggaatg
    18481 aagccgtagt tggtcggttt ggcgaagatg gcgggttcga cgcggtcgag ttggaatgcg
    18541 gccagtttgc ggttccactc gattttgtgg ttgctgccgg cggggatttc gttgacgacg
    18601 ttgatgatgc cgccgtccac atcgccgggg gtcaggattt ggttgaagtc tgccatttgg
    18661 tttcctttgt ttgggaaagt ttgaagtttg aaagtatagc acaaacgtcc ggctgaaaat
    18721 gcgcccgatg ccgtctgaaa gggtgtacgg gcgcgtgtta ccgtttgccc aaaaacctgc
    18781 ccagttccag aatcgcgcgc ctgttggacg gggaaaacac tttttccgcc gcttcttcca
    18841 agccgaacca gccgtaggag acgtgttctt cgggttgcag ggcgatgggc gtatcacgcg
    18901 ggatttcggc agagaagagg tgttcgcggt tttcaaacac gccttttgga tagcggtgcc
    18961 gccagtggtg gtagatttcg taaaccgtgc tgtcgtgcca gtcttgaagc tgcccgtccg
    19021 ccagcaggat gccggtttct tcccaaactt cgcgccttgc cgtttgggcg acggtttcgc
    19081 ccggttcgag gctgccggtt accgactgcc aaaatccttc cggatgcgtg cgttcgatga
    19141 gcaggatgcc gccgtccccg ctataaagga cgaccagtgc ggagacgggg tatttgagcg
    19201 gttttgccat cggcatcttt cggcgggctg cggtaatgaa ggggcggatt atagcaaacg
    19261 ccgcacgtta tggcgtttat ccttttccgt atcctttttt ctgcacggat gggacgcgcc
    19321 ggtgtttgcc ggtaaatttt ccgttgtgtc aaaaagataa gggcggttgt gattttaatg
    19381 cttgccaaag cgtcgggcgg aaactataat ccgaaactta tcgagtcgga gtgtggcgca
    19441 gtctggtagc gcacttgcat ggggtgcaag gggtcgaagg ttcgaatcct ttcactccga
    19501 ccaaaaattc cgaaagccgc tttcaaaagc ggcttttttg ccgtccgtat gattatgatg
    19561 tagagtacgc ggcgacagac attcaaatgc cgtccgaaaa ccgttcagat ggcatttctt
    19621 tatctt
//
LOCUS       DAXHTR010000018        19374 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.018, whole genome shotgun sequence.
ACCESSION   DAXHTR010000018 DAXHTR010000000
VERSION     DAXHTR010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19374
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.018"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(173..2779)
                     /gene="acnD"
                     /locus_tag="ACFNML_000464"
     CDS             complement(173..2779)
                     /gene="acnD"
                     /locus_tag="ACFNML_000464"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HFC6391901.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNREIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELTGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRLGLKRKPWVKSSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKTQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            complement(2857..3645)
                     /locus_tag="ACFNML_000465"
     CDS             complement(2857..3645)
                     /locus_tag="ACFNML_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HFC6391902.1"
                     /translation="MEDLYIILALGLVAMIAGFIDAIAGGGGLITLPALLLAGIPPVS
                     AIATNKLQAAAATFSATVSFARKGLIDWKKGLPIAAASFAGGVVGALSVSLVSKDILL
                     AVVPVLLIFVALYFVFSPKLDGSKEGKARMSFFLFGLTVAPLLGFYDGVFGPGVGSFF
                     LIAFIVLLGCKLLNAMSYTKLANVACNLGSLSVFLLHGSIIFPIAATMAVGAFVGANL
                     GARFAVRFGSKLIKPLLIVISISMAVKLLTDERNPLYQMIISMF"
     gene            complement(3806..4960)
                     /gene="prpC"
                     /locus_tag="ACFNML_000466"
     CDS             complement(3806..4960)
                     /gene="prpC"
                     /locus_tag="ACFNML_000466"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFC6391903.1"
                     /translation="MTETTQTPTLKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKTKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPERESHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     DEETIGGHFLQLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            complement(5046..5921)
                     /gene="prpB"
                     /locus_tag="ACFNML_000467"
     CDS             complement(5046..5921)
                     /gene="prpB"
                     /locus_tag="ACFNML_000467"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFC6391904.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADAVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            complement(6547..7857)
                     /locus_tag="ACFNML_000468"
     CDS             complement(6547..7857)
                     /locus_tag="ACFNML_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HFC6391905.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGHFRVQGAIIITILTITVIASLMGLN
                     EFHGIIGEVPSIAPTFMQMDFEGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(7996..9174)
                     /gene="ftsZ"
                     /locus_tag="ACFNML_000469"
     CDS             complement(7996..9174)
                     /gene="ftsZ"
                     /locus_tag="ACFNML_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFC6391906.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(9293..10537)
                     /gene="ftsA"
                     /locus_tag="ACFNML_000470"
     CDS             complement(9293..10537)
                     /gene="ftsA"
                     /locus_tag="ACFNML_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFC6391907.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(10623..11351)
                     /locus_tag="ACFNML_000471"
     CDS             complement(10623..11351)
                     /locus_tag="ACFNML_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HFC6391908.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYASDGLPEKESEE"
     gene            complement(11341..12255)
                     /locus_tag="ACFNML_000472"
     CDS             complement(11341..12255)
                     /locus_tag="ACFNML_000472"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFC6391909.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKEKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(12369..13778)
                     /gene="murC"
                     /locus_tag="ACFNML_000473"
     CDS             complement(12369..13778)
                     /gene="murC"
                     /locus_tag="ACFNML_000473"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFC6391910.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVVTNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(13933..15000)
                     /gene="murG"
                     /locus_tag="ACFNML_000474"
     CDS             complement(13933..15000)
                     /gene="murG"
                     /locus_tag="ACFNML_000474"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFC6391911.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGREGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(15004..16329)
                     /locus_tag="ACFNML_000475"
     CDS             complement(15004..16329)
                     /locus_tag="ACFNML_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HFC6391912.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(16455..17792)
                     /gene="murD"
                     /locus_tag="ACFNML_000476"
     CDS             complement(16455..17792)
                     /gene="murD"
                     /locus_tag="ACFNML_000476"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFC6391913.1"
                     /translation="MTLENKKILVAGLGGTGISMIAYLRKNGAEVAAYDAELKPERVS
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLSPTAATVLNISEDHLDRYDDLLDYAHTKAKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEHEADFWLERETGRLKQGNEDLIATKDIPLQGLHN
                     AANVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGKKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDAPQIRRDLDGCGLN
                     MTDCATLEEAVQTAYAQAEACDIVLLSPACASFDMFKGYAHRSEVFIEAFKAL"
     gene            complement(17882..18154)
                     /locus_tag="ACFNML_000477"
     CDS             complement(17882..18154)
                     /locus_tag="ACFNML_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HFC6391914.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDLVLAQEWFGKACQNGDQDGCDIYQRLKAGY"
     gene            complement(18269..19351)
                     /gene="mraY"
                     /locus_tag="ACFNML_000478"
     CDS             complement(18269..19351)
                     /gene="mraY"
                     /locus_tag="ACFNML_000478"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFC6391915.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIASLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYA
                     SGHSQFAQYLQLPYVAGANEVVIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         4629 a         5366 c         4899 g         4480 t
ORIGIN      
        1 atttgtccgc acagaaactc atcggataaa taaggtttcc tcaattccac tttctggatt
       61 cccgcctgcg cgggaatgac gatttacagt agatgcctgc catatcgaga attacgtgaa
      121 tgagcaaaat gttgaacccg acccacgcta cgtcttgctt ttcagacgac ctctaagccg
      181 cgttcccttc caaaaaatcc tgtgcaaacc gttgcaacac gccgccggct tcatatacca
      241 atacttcttc tgcagtatcg aggcggcagg taacgggaac ttcgacggtt tctccgtttt
      301 tacggtgaat cacgagggtc aggtcgcagc gcggtgtgcg ttcgccgacc acgtcgtagg
      361 tttccgtacc gtccagttgc agggtatggc ggttggtgtc gggtttgaac tgcagcggca
      421 acacgcccat gccgataagg ttggtgcggt ggatacgctc gaagccttcg gcaacaatcg
      481 cttctacgcc ggcgaggcgt acgccttttg cagcccagtc gcggcttgag ccttgaccat
      541 agtccgcacc ggcaatgatg atgagcggct gtttgcggtt catataggtt tcgatggctt
      601 cccacatgcg catggtttcg ccttcgggtt cgacgcgggc gaacgagcct tggcgcacgc
      661 tgccgtcttc gtttttcacc atttcgttaa acagtttcgg attggcgaag gtagcgcgtt
      721 gggcggtcaa gtggtcgccg cggtgggttg cgtaagagtt gaagtcttct tcaggcaaac
      781 ccattttcgc caaatactcg cctgcggcac tgacggccaa aatcgcattg gacggcgaga
      841 ggtggtcggt ggtgatgttg tcgggcaaaa tcgccagcgg acgcatacct cttaatgtgc
      901 gttcccctgc cagtgcgcct tcccagtaag gtgggcggcg gatataggtg gacatcggac
      961 gccagtcgta cagcggacta ggtgcttttt gcgctgtgcc ggtgtcgaac atcggtacat
     1021 acacatcgcg gaactgctgc ggtttcacat attcggcaac gacggcatcg atttcttcat
     1081 cggcaggcca aatgtctttc aggcggattt ccttgccgtc tgcaacgccg agtacgtcgt
     1141 tttcaatatc gaaacggata ctgcctgcca gcgcgtaggc aacgaccaac ggaggcgaag
     1201 cgaggaaagc ctgtttcgca tacggatgga cacggccgtc gaagttgcgg ttgcctgaca
     1261 gtacggcggt ggcgtacaaa tcgcggtcga tgatttcttt ctgggttttc ggatccagcg
     1321 cgccactcat gccgttacag gtggtgcagg cgaaggcgac gataccgaag ccgagttttt
     1381 ccatttcggg cagcaggcct gcttctttca aatagatttc ggctactttt gaacccgggg
     1441 caaatgaaga tttcacccaa ggtttgcgtt tcaagccgag gcggttggca ttgcgtgcca
     1501 acagcgcggc ggcaacgacg ttgcgcgggt tggaagtgtt ggtgcaactg gtaatcgcgg
     1561 cgatgatgac cgcgccgtcg ggcatttgtc cgtccgaagg ctcttcgtaa ggcttcgcca
     1621 gccctttcgc cgccaaatcg gcggtcgcaa aacgggcatg cgggttactt ggacctgcca
     1681 tattgcgcgt tacgctgctc aaatcaaatt tcaaaacgcg aggataaacg gcggttttca
     1741 aggcatctgc ccataagcct gcggttttgg cgtaggtttc caccaatttc acctgcgcgt
     1801 cgtcgcgtcc ggtcagtttc aaataatcaa tggtttgctc atcaatagcg aacatcgcgg
     1861 cagtcgcgcc gaactccggc gtcatgttgg aaatggtcgc gcggtcgccg atagacaggc
     1921 ttctcgcgcc ctcgccgaag aattcgacaa acgccccgac cacgcgctct ttgcgcagaa
     1981 actcggtcag cgccaacaca atatccgtcg ccgtaatgcc cgcctgccgt ttgccggtca
     2041 gctccacacc gacaatatcg ggcaggcgca tcatggacgc gcgtcccagc attacggttt
     2101 ccgcttccaa tccgcccaca cccacggaaa tcacgcccag cgcatcgacg tgcggcgtgt
     2161 gcgaatccgt gccgacgcag gtatcgggga acgccacgcc gtttttaact tgaacaaccg
     2221 gcgacatttt ttccagattg atttggtgca tgatgccgtt gcccgccgga atcacgtcca
     2281 cattttcaaa tgcggttttt gtccagttga taaagtggaa acggtcttcg ttacgtctgt
     2341 cttcgatttc gcggtttttg cggaaggcat caggatcgta accgccgcat tcaacggcca
     2401 aagagtggtc gacaatgagc tgggtttgca ccaccggatt cactttggca ggatcgccgc
     2461 ctttttcggc aatcgcatcg cgcagacctg ccaaatccac caacgcggtc tgccccagaa
     2521 tatcgtggca caccacccgc gccggatacc aaggaaagtc gatttcctgt tttccctcaa
     2581 tcagctgacc cagccagctt tgcagcgtcg gcaaatcgac tttgtccgcg cggttgacca
     2641 aattctccgc caaaatgcgg ctcgtgtaag gcagcttgtc gtaagagccg ggcttgatgt
     2701 cctcacacgc cgcacgcgcg tcgtagtatt ccaaatccgt accgggcagc ggtttgcggt
     2761 aacgttggtt ggcagccatg tcggttctcc tggggatctg tttttcttgt ggtttgaggt
     2821 ttcagccgac gttttgaagg ggtcgtctga aagggtttaa aacatcgaaa taatcatctg
     2881 atacagcgga tttctctcgt ctgtcaacaa tttcacagcc atcgaaatgc tgatgacaat
     2941 cagcagcggc ttaatcagct tcgaaccgaa gcggacggca aatctcgcac ctaaattcgc
     3001 accgacaaac gcaccgaccg ccatcgttgc cgcaatcggg aaaataatcg aaccgtgcag
     3061 caggaatacc gatagcgaac caagattgca ggcaacgttc gccaatttgg tgtaagacat
     3121 cgcgttcaac agcttgcagc cgagcaaaac aataaaggca atcagaaaaa acgagccgac
     3181 acccggtccg aacacaccgt cgtaaaaacc caaaagtggt gcaaccgtca gaccgaacag
     3241 aaaaaaagac attctggctt tgccttcctt actgccgtcg agcttgggcg aaaacacaaa
     3301 atacagcgcg acaaatatca acaaaaccgg cacgaccgcc agcagaatat ctttggaaac
     3361 caagctgacc gataatgcac cgaccacgcc gcctgcaaac gatgctgccg caatcgggag
     3421 acctttcttc caatcaatca aacctttgcg tgcaaaagaa accgtagccg aaaacgtagc
     3481 agcggctgct tgcagcttgt tggtggcaat tgccgacacg ggaggaatac ctgccaacaa
     3541 gagtgcaggc agcgtaatca aaccaccccc acccgcaatc gcatcgataa atccggcaat
     3601 catcgcaacc aaacccaaag cgagtattat gtataaatct tccatgtttc ttatcctgtt
     3661 acttgcacca atacaggatt atcttcttct attaggttaa atttttaaac ttatttcaga
     3721 caacctttcc aatcaggcaa ggtcgtctga aatcctcagc tttgcatacc gaaattaaag
     3781 aacaaactgc tattgcattc ttcaattatc gttcttcaat ctccacaaac gccaaatctt
     3841 cagggcctgt gtagtttgcg ctcggacgga tgattttgcc gtctttgcgt tgctcaagaa
     3901 cgtgtgcgct ccaaccggtt gtacgggaaa ttacgaacag cggtgtgaac atagcggtcg
     3961 gtacgcccaa tttttggtag gaaacggcag agaaccagtc cagattcggg aacatttttt
     4021 tctcttccca catcacgctt tccaaacgtt cggcaatgtc aaagaggcgc atatcgccgg
     4081 tttctttgct caaaccgcgt gccacttctt taatgacaac gttgcgaggg tcggaaatgg
     4141 tgtacaccgg atgaccgaaa ccgatcacga tttctttgcg gccgatgcgt tcgcggatgt
     4201 cggcttcagc ttcgtcggca ttgcggtagc gtttttgaat atcgtaagcc acttcgttcg
     4261 cgccgccgtg tttcggacct ttcaacgcgc cgattgctcc ggtaatgctg gagtacatat
     4321 cagagcctgt accggcgatc acgcgggcgg taaaggtaga agcgttgaac tcgtgttcgg
     4381 catacagaat cagtgaaacg tgcatggctt tgatgtgtga ttcgcttggg cgtttgccgt
     4441 gcaacagttg caggaaatga ccgccgatgg tctcttcgtc gctttcaacc tcaatgcgtt
     4501 tgccgttgtg cgaatattga taccagtaca agaggatgct gccgaggctg gcgatcagtt
     4561 tgtcggcgat gtcgcgcgct tcactttccg gatggctttc acgttcagga tgaacgcagc
     4621 ccagcatgga tacgccggta cgcattacgt ccatcggatg ggtatgtgca ggcaggcttt
     4681 ccaaaacttt aatcacacgg ataggcaggc cgcgcatgga tttgagcttg gttttataag
     4741 cggccagctc gaatttgttg ggcagatggc cgtgaatcag caggtgggcg acttcttcaa
     4801 actcgcattt ttgtgccaaa tccagaatgt cgtaaccgcg atagctcaaa tcgttgccgg
     4861 tacggccaac ggtacacaaa gcggtattac cggccgcaac gccagaaagc gcaacggatt
     4921 ttttaggttt gagggtcggg gtttgagtag tttcagtcat ggtatttctc ctttgtgttt
     4981 ttatgggttt cgggttttca gacgaccgat gcggatttgt tgaaaggcag tctgaaagcg
     5041 gtaaatcatt tttgaaacaa tttatccagt ttttgctcga aggcatgata gttcagatgc
     5101 tcgtacagct cggcacgggt ttgcatactg tccaccaccg ccgcctgagt gccatcgcgc
     5161 ataatcgctt cgtaaacatt cagagcggct ttgcttgctg cacggaacga tgacagcgga
     5221 tacagcacca gcgacacgcc gttttcagcc agctcgcttt gggtataaag cggagtggaa
     5281 ccaaactcgg taatgttcgc caacacgggc actttcaccg catctgcaaa ttggcggtac
     5341 atgttcaaat cggtcatggc ttcagggaaa atcatgtccg caccggcttc gacacaagct
     5401 tgggcgcgtt cgatagcggc atccaaacct tctaccgcca gcgcatcggt acgcgccata
     5461 atcacgaagt tctcatcaac gcgcgcatct acggcagctt tgatacggtc gaccatttca
     5521 tctttagata caatggcttt gttcggacgg tggccgcagc gtttttgcgc tacctgatct
     5581 tcgatgtgaa ccgctgcaac accggcgcgt tcaaagttgc gaatggtacg ggcaatattg
     5641 aatgcaccgc cccaacccac atcgatgtcc accagcagag gcgtatccac gttgtccgta
     5701 atgcgtcgtg cgtcgatcag cacatcttcc attgtggtaa tgcccaaatc agggataccg
     5761 caagaacagg ctgccacgcc gccgccggac agatagatgg ctttgaaacc gctttgggtg
     5821 gccaatcgtg caaaataagc attgacgcaa ccggcgacgg caagcggatt cgattctttc
     5881 acggcttggc ggaaacgtgc tccggcagag tgttgactca tcatatttct cctttataga
     5941 ctttttttca gtatggacag gcttccatca cattcggacg gcaaaacaca gccatccgac
     6001 gcgtcgggca tcccaatcca atcattaaaa atatatggga aaaattatct tattgatatt
     6061 taaaacgaat caaagaaaaa cagcagaccg ttcggaatta tgcggcaaaa ccgcagacaa
     6121 gaagaaaaca aggggattat tcagaaaagg ggaaaacatc tgaattggtt tcatagtaat
     6181 gttcctttgt agtgttatgt agttttattt ttcgataatc taaaacacaa gactgaaaat
     6241 gtcaaacatt tttaaggaga ctcattacat aattttaata ttttatttta attccatgat
     6301 taaactagat taattttgaa ttatttgttt caataaaatt gtcaggaaat caagaaaaat
     6361 tttcgataat caaaatcctg aaaacttata aaatatttta tacataaaaa caatgtatta
     6421 tttttaagca atccgatgta ttttattata tatttgattt ttatataatt taataaaaat
     6481 taaatatatc ggcaaacaaa atgccgtctg aaaacctttc agacggcatt tttaatatcg
     6541 aatcaatcag cccaaatacc agaatttcag tgcccacaat acggcaacaa tccataccat
     6601 aggcggaacg tctttggtgc ggcggcataa aagtttaacc acggcatagc tgatgaagcc
     6661 gaaggcgatg ccgtctgcaa tcgaataggt aaacggcatg aagacaatgg tcaggaatgc
     6721 gggtgcggct tccgtcatat cgtcccaatc aatatccctc gcactgcgga gcatctgcgt
     6781 gccgacataa agcagggcgg gcgcggtggc aaaagcggga acacttttcg ccaaaggtga
     6841 aaacatcagg caggcgagca tcaatacgcc gacggtaacc gccgtcaggc cggtccgtcc
     6901 gcctgccgat acgcccgccg cgctttccac ataaggcgtg gtggaagaag tacccaaagc
     6961 cgcacctgcc acaatggcgg tagagtctgc aagcagtgcg cgtttcaggc ggggcagctt
     7021 accgtccacc agcagcccgg cacggtggga tatgccgacc agcgttccgg tactgtcaaa
     7081 tagatcgacc aagaagaaga cgaaaatcac actgaccatg ctgacggtaa acaggccttc
     7141 aaaatccatc tgcataaaag tcggcgcaat gctcggtact tcgccgatga tgccgtgaaa
     7201 ttcattcaaa cccatcaggc tggcaatgac ggtaatggtc aagatggtga tgatgattgc
     7261 gccttgaacg cggaaatgtc ccaatacgac caccatagca aaaccgaaca atgccaacaa
     7321 cgcggacggc tgatgaatat cgcccaaacc gaccaaggtt gccggattgg caacgataat
     7381 gcctgcgcct ttcagggaaa tcagtgccaa aaacaaaccg ataccggcag caatcgacat
     7441 tttcaaaccc ataggcagtg cgttgaccag catttccctg actttaaaaa agctgaacag
     7501 gataaaaatc agaccggaga tgaacaccgc acccaacgca acctgccaag gcacgcccat
     7561 acccttaacg acggcaaagg tgaaataggc attcagcccc atccccggtg cgagtgcaat
     7621 cggatagttg ccgacaaaac ccataacaaa acagccgatg gcagacgcga tacaggtagc
     7681 gacgaatacc gcccccatat ccatgccggt ctcgcccaaa atcagagggt tgacgataac
     7741 gatgtagcac atcgtcaaaa aagttgtcaa acccgccatc aactcggtac gcaccgtcgt
     7801 accgtttgcc ttcagcttaa aaatcccgtc caacagtgtt tgttttgaag tgtccatatc
     7861 tgaaagtctg tatcaagtgg aaaaatctga atccgccgac cgcaaaccgt acgaaattgc
     7921 aacatcttga aaaacatacc tcccaaaccg gacaaaccga ttcgggaggc agcaggttta
     7981 cgggcaaaca gcacattatt tgtctgaatt gtgttgacga cgcaaaatcg cagggatttc
     8041 aaagtcgtca agtacggact gattgtcgaa atccgcagcg gtaaggttca tcgtgcggat
     8101 accgcgattg gtgcggatca taccttcgac attgtggctt tgctcctgtt tggacggagc
     8161 aaccgcttct acctcccttg ccggaacaaa atcgaccgcg cctttttctt tcagaccggt
     8221 agcgataatg gtaatccgga tggcatcttc gctcatggtc tcgtcttcag ccgcaccgaa
     8281 tttgcattcc aaatcgggat gcgcgctttg gttgacgatt ttcatgactt cggacaactc
     8341 ggacattttc aagcaacccg gagcagtcgt aatattgacc agcacaccgc gcgctccgtc
     8401 caaggttaca tcgtccagca gcggactgga aatggcctgg tcggtcgcca tacgcgcacg
     8461 gtcgatacct tgggcataac ccgaacccat catagcgata ccgcggttgc tcatcacggt
     8521 tttcacgtcg gcaaagtcga ggttgatgat ttcgctcggg caagttacca cttcggaaat
     8581 gcctgcgacc gcatcgcgca atacattgtc ggcggcacgg aaggcttcgc gcatcgttac
     8641 gtcttcaccc aatgcagtca tcagtttgtc gttcgggatg atgatcagcg aatcgacgtg
     8701 ttctttcaac tgttccaacc ctgcctgtgc gacatggacg cgcttacctt catatgcgaa
     8761 cggtcgggta accacggcaa cggtcagaat gcccaaagac ttggcaatct cagcaacaac
     8821 cggcgcggaa ccggtaccgg taccgccgcc cataccggtc gtgataaaca gcatattcgc
     8881 accgcgaatg gcttcttcaa tggcttcccg gtcttcctgg gctgccgcac ggccgatatc
     8941 gggattcgcg cccgcgccca aaccgcgtgt cagattcgta cccaactgga ttctcttcgc
     9001 cgcatggttt tttgccagag actgcgcatc cgtattggca ctgataaact ccacaccgcg
     9061 cacattgttg gcaaccatgt tattgattgc attgcaaccg ccgccgccca agccgattac
     9121 tttaatcacc gcagggctga ctgccgattc tgccacgtcg taaacaaatt ccattcaaaa
     9181 actcctgctc gccccattca gaggacggtt taaataaatt attattcatt atataagaag
     9241 tatcttgctg ctggcaaaat acttctcacc tgtcaaacgg caatccacct gttcagaagc
     9301 tgttttcaat ccaccgtttc aatcttgcca acaaaccgcc gcccccttcc ctctcttgca
     9361 ctgcaccgtt ttccggctgc ggcaagtttc cttccagctt gcatgctgca tgaagcagcc
     9421 cgatggcggt agaaaagcgc ggtgtgcgga cgcggtcgga caaaccgccc atttcttggg
     9481 gcgcaccggt gcgtacaggc aaatcgaaga ttttttcggc aaattccaca atcccggtca
     9541 tcatggacac accgccggtc agaacgatac ccgcattcag cacttctttg gggaaacccg
     9601 atttttgcag ctcgcccagc actacgccaa aaatctcctg aatacgtgcg ctgatgattg
     9661 ctgccaaaac cttactggaa acctgacgcg atgtccggtc acccacgccc ggaacttcaa
     9721 tcatctcacc caagccttcc gtatcgcatg atgccacgcc ataatggatt ttaatgtact
     9781 cggcggcatc gagaggtgtt ctcaacgatt tggacaaatc tttggtaatc agattaccac
     9841 cggccggaat gacggacgta tggcggatgg caccgttcat ataaacggca atatcggtcg
     9901 ttccgccacc aatgtcgatg acgcatacgc cgaggtcttt ttcatcttca gtcagcaccg
     9961 cctgcccgct tgccaacggc tgaagcatga tctgatcgct tttcaaaccg caccgctcga
    10021 tacatttttg gacattctgc actgccgtac ttgcaccggt aatgatgtgc acccgcgtat
    10081 ccagacgcac accgctcata ccgatgggct ccctcacgcc aagttgggtg tcaataatgt
    10141 agtcttgaac cacggcatcg agaatttttt gatcgggcgg gatattgatt gcctttgccg
    10201 tttcaatggc gcgatcgatg tctgcctgcg tgacttcccc atctttaatt ttaaccacac
    10261 cttgcgaatt gagactgcgg atgtggttgc ctgcgatacc tgtggtaacg tgagtaattt
    10321 tggtatccgc catcagctcg gcatcattga ccgcctgcct gatggcttgg acggtggcat
    10381 cgatattggt taccatgccc gcgcgcaagc cccgtgaagg agcctgcccc aaaccgacga
    10441 tgttgatttt gtcgtcatct tgaacttccc cgatcagtgc gaggacttta gacgtaccga
    10501 tatccagtac gctgatgtat ctttgctgct gttccattgt tcgtctgctc ttaaaactga
    10561 ttgaaatttg cgtcgcaccg tttcagacgg cacggccgta atctgtccga tacctgttcc
    10621 cactattctt cggattcttt ttcgggtaaa ccgtcggaag catagcggac tgaaaatccg
    10681 tccttatacc tcatatccac ataggataac cgatttttat ttttacgcaa cagatgctgc
    10741 cacgcttcgg taaaaagccg gaggcgtttc atctcgtttt cccttccgag cctgacggtg
    10801 atgccgttgt ccaaaacgac aatccacgcc gaacgtgccg tataggtcat ctctttgatg
    10861 cccaaaccct gttttgccaa aacagtcgaa aattcgtcat aacggcggag catttcggca
    10921 gacgttcctt ccgcgcctct gaataccggc attccgggtc tgtccaagcg ggcttcaaaa
    10981 acattgcctt cgccgtccac caaggcatgg tcgccccaac gcgcgaccgg cttgcgctcg
    11041 gtcaggacga cctcaaccgt gtcgggaaaa cggcggcgca ccatgaccga cgcaatccac
    11101 ggataccggc ggtaggcctc ctgtgcgcca ttgatgtccg tcctcaaaat attcccatgg
    11161 atgtattctt tcgccaaact gcccaatgct ttcttatcgg aataaaccag gttgcccttc
    11221 agcgacacct gcttgacggg cagatgattc gaattgtaaa accaaaccag cccggacgca
    11281 gcaagcagca tcgccatcat gacaagcagc cagcgcgtca gccgttccat cgcttcggca
    11341 ttatcccaca tgtgcggtct tcaaaatttc aatacataaa tcggcaaaac ccacgcccgt
    11401 aacggcagcg gatttcggta ctaaactatg gctcgtcata ccgggcaggg tgttgatttc
    11461 caacagatag agtttgccgt cggtatcttt gaggaaatcg acgcgcacgc agccttccgc
    11521 accgattgcc tgcgcgccgc gaaccgccag ttcgcgcatc aggctttctt cggcttcggt
    11581 cagatcttcc gaaggacatt gataaatggt gtcgtcgcgg ttgtacttgg cttcgtagtc
    11641 gtaaaactcg gtcgcgggga tgatgtgtat gccgggcagg cctttgccgt tcaggacggg
    11701 gcaggaatat tcgccgccgc cgataaaccg ttcggcaatg atttcgccct gaaggtgttt
    11761 caattcttcg taaacgcttt tcagacggcc tttttctttg acttttacca caccgacgct
    11821 gctgccttcg gccgccggtt tcacaaacat cggcaggccc aatttttctt cgacggcatc
    11881 gaaatcagtg tcgtcgtgca ggacggcgaa ctcgggaacg ggcagcccca atgcctgcca
    11941 aatcagtttg cagcggtatt tgtccatgcc gatggcggag gcggcgacac cgctgccggt
    12001 atagggaatg cccaacagtt ccaacgcgcc ttgaaccgcc ccgtcttcgc cgtaagtacc
    12061 gtgaaggatg ttgaatgccg tctgaaaacc ttgtgccttc aattcagaca atggggtttc
    12121 cttgggatcg aaggcgtatg cgtctatgcc tttgcttttt aaagcgttca aaatggcggt
    12181 gccgctgtcc agcgagattt ctcgttcgct ggaaaaaccg cccatcaata cggccacttt
    12241 gccaaaattc tgcattgttt tttgttcttt cctgattgct ttatgcttgt tgccagaggt
    12301 cgtctgaaac ctgatttgcg gtttcagacg acctttatat gatgttccgt ctgtcaggag
    12361 ggtgtgcctc aaatctgttt cgacaattcc agtagcgcgg cgggaacgcg gttgatgctt
    12421 cccgcgccca tattcaacac gatgtcgccg tcctgcaaaa cgttcaacag catttcgggc
    12481 agatcggcaa cgttttcgca gtaaatcggc tcgagtttgc ccaacacgcg gatggcgcgg
    12541 gcaagagcgc gggaatcggc ggcggcaatc ggctcttcac cggcggcata aacttcggtc
    12601 agcaccagcg cgtcaacggt attgaggact ttggtaaagt cttcaaacaa atcgcgcgtg
    12661 cgggtatagc ggtgcggctg gaaggcgagt accaaacgtt tttccggata cgcgccgcgt
    12721 gcggcggcaa gggtcgccgc catttcgacg gggtggtgtc cgtagtcgtc caccaagagc
    12781 gcggtcccgc cgtttggcaa cttgatgtcg ccgtattttt ggaagcggcg gccgacgcct
    12841 tcaaagccga gcaagccttt ttggatcgct tcaaccgatg cgccgacttc cagcgccacg
    12901 ccgatggctg ccaatgcgtt cagcacgttg tgtctgccgg gcatattcag cacgacttca
    12961 aacgacccct gctcatgtcc tttcatttga acatggacgg tgaatttcat ttgcgcgccg
    13021 acgttttcga tgtcggtggc gtagatgtcg gcggtatcgt ccaaaccgta agtagcataa
    13081 ggtttgctca ctttgggcaa aatcgcgcgg acgtgttcgc tgtcaataca caaaaaggct
    13141 ttgccgtaga agggcatacg gtggatgaaa tcgataaacg cctgatgcag cttctcaaca
    13201 ctgtgcccgt aggtatccat atggtcttcg tcgatattgg taacgacgga cataatcggt
    13261 gtcaggtgca gaaaggatgc atccgactcg tcggcttcgg caacgatgta ttcgcctttg
    13321 cccaagcggg cgttggtgcc tgcggcgttg agtttgccgc cgataacgaa agtcgggtca
    13381 agtcctgccg cgccgaggat ggaggcggtc aggctggtgg tcgtggtttt gccgtgcgtg
    13441 ccggcaatgg cgatgccgtc acggaagcgc atcaactccg ccaacatcag ggcgcgcgga
    13501 ataacgggaa tttgctgctc caacgcagcg acaacttcgg gattttcttt tttgacggcg
    13561 gtagaggtaa cgacgacatc cgcaccgtta acgtgttctg cggtatggcc gggataaact
    13621 tgaatgccca ggctgcccaa atgctcggta gcggcatttc gcgcctgatc cgaaccggaa
    13681 actttaaaac ccaaattgtg caagacttcg gcgatgccgc tcatgccgac gccgccgata
    13741 ccgacaaaat ggatgttggt aactcgattt ttcatcataa tgttgcgttc cggttggttt
    13801 ttagtgcgta aaggcgttat tttaaagggc tgaccgtttg cgcgccatag ttttctgaca
    13861 aatatatagc ggattgaaat aaaaaacatc catgccgtct gaacggcttt tcagacggca
    13921 tggttcggca gtttacgccg cacacgcaat cgcggcttcg gcaacgtcgt ccgcactgtg
    13981 cggcagtgcc aaagtacggg cgttttctgc ccatttgaga catttttcgc ggttcaggct
    14041 gccgaggatt tcggcgagtt tttccgccgt caactgggtt tgcggcaaca gcaatcccgc
    14101 ctccgcctgc accataaaac gtgcgttggc ggtttgatgg tcatcaacgg cgtgaggata
    14161 cggcactaac aacgcaccca atcccgccgc cgtcaactcg gcaatcgtca gcgcgccggc
    14221 acggcaaatc accaaatcgg catcgcggta ggcggacacc atgtcggtaa taaattccac
    14281 acattcggct ttcacgccca gcgcgtcgta atccgcctgc aagctgccca gcttgccccg
    14341 tcccgattgg tggtacatct gcggacgcgc attgtcgggc agcaaagcca atgcctgcgg
    14401 tacggttttg ttcaaaacgt ccgcgcccaa actgccgccg accaccaaaa ttttcagacg
    14461 gccttcacgc ccttggaagc gttcggcagg cacgggcagg ttgctaatat cggcgcggac
    14521 ggggttgccg accaagccgc cttcgtggct gaacgctttc ggaaaagcgt acaacacccg
    14581 cttcgcccag cgcgacaggt ggcggttgga caaacctgcc acggcgtttt gctcgtgaat
    14641 cacaatcggc acgcctaata gcttcgccgc caaaccgccg gggaaggtaa cgaagccgcc
    14701 gaagccgatg acgcactcga cacggtgttt gcggataatc cgctgcgctt cgcggacggt
    14761 ttgatacaaa gtaaacggca gcatcagctt gcgtttgatg ccgttgccgc gcacgccttt
    14821 aatcgccagc gtttccaagc gtatgccgta ttgcggcacg atacgctctt ccatcgaatc
    14881 cttgctgccc agccaaatca catgatggcc gcgcgcgcgc aatgaatccg ccaccgccag
    14941 cgcggggaaa atatgtccgc ccgttccgcc cgccatcagc ataaaggttt taccgcccat
    15001 gatttactcc acccgataac cgcgcatttt ccggcggttt tcataatcta tacgcaacag
    15061 cagcatcatg ctgatcagca tgaaaaagac tgacgaaccg ccataggaca tcaacggcag
    15121 cgtcagacct ttggtcggca aagcaccgat gttcacaccg atattgaaga aactttggat
    15181 accgatccaa atgccgatac ccgaagcgat ataggcgttg aaagtcaaac ccaaatcgcg
    15241 cgactgcttg ccgatggaaa acgcccgcac caccagccag ccgtaacaga atatcagcac
    15301 acacataccg aagaaaccga attcttcggc gatgatggca aaaataaaat cggtatgcgc
    15361 ttccggcaga aagccgcgtt tgctcaaact cgcacccaaa cccataccga accactctcc
    15421 gcgcccgatt gccatcagag agtgggtaag ctggtagccg gcaccctgag ggtctttcca
    15481 cgggtccaaa aatgccacta cccgctgcac acggtaggga gcggcggtaa tcatcagcac
    15541 catcccgccc aagacgctgc ctaccaggac gaaaaaatat ttccacggca atcctgccaa
    15601 aaacagcatt ccaacggcaa tgacggtaat gacgacaaac gaaccgaaat ccggctgtac
    15661 cattatcagc accaaaccga acgccaccag cataatcggc aggatgatcg cccggaaacg
    15721 gccgtacatt tctaatgttt cacgacgtgc ctgcggattg gtggcggaca tgatcagatt
    15781 ggccgtcccc cgccaaatcg actgccaacc caaactttcc atgctgcgca acacttcttc
    15841 acggcgcgtg aacaggcttg ccaaataaag gatgaccgcc agcttgaaca gctcggtcgg
    15901 ctggaaattc aacggaccca aaggtatcca acgggtcgcg ccattgattt cgcgcccggc
    15961 aatcaatacg actaccagca acaggccgga tagggcaaaa atccacggca caagccgccg
    16021 ccatgtcctc atcctgcaaa gaaaccataa aaaaccgctc gctatcaagc cggcaacgac
    16081 gaaccccgcc tgtctggtca aatagaaaaa ctgatcgccg ccttccttcg atgccaaata
    16141 cacagaagcc gaataaatca tcagcaggct gaacgccgtc atcagcacca ccatccacaa
    16201 aagcggcgcg tcgaatttcc tgccgtcgcg cacaatcggt ctgtcgagca gcagagtgtg
    16261 gacaccgtcg cccactttta ccaatacttc cgaaatcttc aaaaaaacca cctgccagtc
    16321 tgtttgcacc tgccgcaaag ggcaaaaatt tcagacggca gacaatgccg tccgaacata
    16381 cgatacatcc caaatcggta ttctaaatct ttacttgccg cccaacaatg acggcgtttg
    16441 catttcagac ggcatcacaa agccttaaac gcttcgataa acacttccga acggtgcgcg
    16501 tagcctttaa acatatcaaa actcgcacaa gccgggctga gcagcacgat atcgcacgct
    16561 tcggcttggg catatgccgt ctgaacggct tcttccaaag tggcgcagtc ggtcatattc
    16621 agaccgcagc cgtccaaatc gcggcggatt tgcggcgcat caacgccgat caggaacacg
    16681 ccttttgcct tgccggcaag cgcgtcgcgc agaggcgtga agtcctgccc tttgcccatg
    16741 ccgcctaaaa tcacgaagag cggattttgc aaaccggcaa tcgcggcggc ggtcgcgccg
    16801 acattcgtgc ctttgctgtc atcaatgaat accacgccgt ttttcttgcc gattttttcc
    16861 acgcggtgcg gcaggccttg gaaggttttg acgtgttcaa gcaatgcttc gcgcggcaaa
    16921 ccgacggctt cgcacaaagc cacggcagcc atgacgttgg cggcgttgtg caagccttgc
    16981 aacggaatgt ctttcgtggc aatcaaatct tcattgcctt gtttcaggcg gcctgtctca
    17041 cgttccaacc aaaaatcggc ttcgtgttcc aacgaaaacc attttacctc gcgcccggca
    17101 cgtttcatgg cacggcagaa cgcatcgtcc gcattcaaaa cctgcacacc gtcgccacgg
    17161 aaaatcttgg ctttggtatg cgcgtagtcg agcaagtcgt cgtagcggtc gagatggtct
    17221 tcggaaatat tcagtaccgt cgccgccgtc gggctcaggc tttcggtgtt ttccagttgg
    17281 aagctggaaa gctccaacac ccacacgtcc gcctttttgc cttcgcgctg ccattccgcc
    17341 tccaaaaccg gcgtgccgat attgcccgcg ataacggtat ccagcccgca cttgatacag
    17401 agatagccga ccaggctcgt taccgtggtt ttgccgttgc tgccggtaat cgcaattacc
    17461 ttgtcgcccc ggcggttcac aatgtccgcc agcaattcga tgtcgcccaa cacgcgtccg
    17521 ccgttttgct tgaacgcctc aatatccggc tgacgctcgc tgatgccggg gctgagtgcc
    17581 aaaatatcga aaccgttgtc cagcgcatct ttcagacggc ctgtgtaaaa caccagcccg
    17641 tcaaacatct taccgatttg cgacacgcgc tcgggcttca actccgcatc ataagcggcc
    17701 acctccgcgc cgtttttgcg cagataggca atcatggaaa tacccgtacc gccgagtccg
    17761 gcgacgagga tttttttgtt ttcaagcgtc attttggttt tgtcctaaaa caaatcatat
    17821 tgggcaggtg gggggatgtc cgcccctgcc caagccgctt tcagacggca tcgcgggctg
    17881 ttcaataacc tgccttcagg cgttggtaaa tgtcgcagcc gtcttggtct ccgttttgac
    17941 aagccttacc aaaccattct tgagcgagca caaggtcttg gcgcacgccg cgtcctttgt
    18001 aatacattgc gcccaaattg tattgggctg ctgcatttcc ctgtgctgcc gcctgcaagt
    18061 tttcccgaaa atccgatacg tcatccgccc acaccgcttg gttcaagccc aaggcaatca
    18121 gggcggcggc aagccatttg actgtctgtt tcatggtttt acttctgttt tagtataagc
    18181 cgccgttaac gatagggctg ggcggattgt cgccgcaggt ttattgcgcg ttcaaatgcc
    18241 gtctgaaagg tgttcagacg gcataggttc agcggatttt gagggtactc aaaccgatca
    18301 acaccaagac gatggtaata atccaaaagc ggacgacgac ttgggtttct ttccagcctt
    18361 tttgttcgta gtggtgatgg atgggcgcca tcaggaagat gcgttttttg gttttcttat
    18421 accagccaac ctgaagcata acggatacgg cttctacgac aaataatccg cccataatga
    18481 cgaggacaaa ctcttggcgg acgatgacgg cgacggtacc gagcgcggca cccaatgcca
    18541 atgcaccgac atcgcccata aagacttgcg cgggataggc gttaaaccac aagaaaccga
    18601 ggcacgcgcc gcacatggcg gtacagaaaa tcaccacttc gtttgcgccg gcaacgtaag
    18661 gtaattgcag gtattgggca aattgtgagt ggccgctggc ataggcgaag atggcgaggc
    18721 cggcggcaac gaggacgacg gggaaggtcg caaggccgtc caagccgtcg gtgaggttga
    18781 cggcattgga tgtgccgacg atggtcaggt aagacaacac caaaaagccg accacgccca
    18841 gcggcagggc gatttgtttg aagaacggga caatcaaaat attgttggcg gaattggcgg
    18901 caaggtaaaa caatgccaaa ctggcgataa tggcaacgct tgactgccac accattttga
    18961 atttggcgga cacgccgttg gggtctttat agacgacttt gcgccagtcg tcgtaaaaac
    19021 cgagtgcgcc cgtggcgagc aatacgccca agagaatcca gatatacggg tttgcccagt
    19081 tgccccacaa cagggtggac acggtaatgg cggtcagaat cagcgaaccg cccatcgtcg
    19141 gcgtgccgtt tttgacgagg tgggtttgcg gaccgtcggt acgcactgcc tgcccgcatt
    19201 tgagcgcggt cagcctgcgt atcgtccacg ggccgaacat cagggaaaac gctaaggcgg
    19261 tcaacgccgc catgacggcg cggaatgtgg tgtattgaaa aatattcaga ccggttaacc
    19321 agttgctgaa atgtgcgagc cataaaaaca tggggcttcc tttttttgtt ttgt
//
LOCUS       DAXHTR010000019        19051 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.019, whole genome shotgun sequence.
ACCESSION   DAXHTR010000019 DAXHTR010000000
VERSION     DAXHTR010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19051
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.019"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            198..1016
                     /gene="opc"
                     /locus_tag="ACFNML_000479"
                     /gene_synonym="opcA"
     CDS             198..1016
                     /gene="opc"
                     /locus_tag="ACFNML_000479"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HFC6391916.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHISVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSSDVSLTIPKTSIREYGLRVGIKF"
     gene            1179..1823
                     /locus_tag="ACFNML_000480"
     CDS             1179..1823
                     /locus_tag="ACFNML_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFC6391917.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            1842..3752
                     /locus_tag="ACFNML_000481"
     CDS             1842..3752
                     /locus_tag="ACFNML_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6391918.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGLQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELGLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLVVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQSAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGDKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVSGKKKHVMSYLEDFLFHPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSI
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKRNGNAV"
     gene            3772..4137
                     /locus_tag="ACFNML_000482"
     CDS             3772..4137
                     /locus_tag="ACFNML_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391919.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4248..5723
                     /gene="trpE"
                     /locus_tag="ACFNML_000483"
     CDS             4248..5723
                     /gene="trpE"
                     /locus_tag="ACFNML_000483"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFC6391920.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVIDNLSGKIHLVVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSPLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIRAAQMVQEGLDK"
     gene            5720..6266
                     /locus_tag="ACFNML_000484"
                     /pseudo
     CDS             5720..6266
                     /locus_tag="ACFNML_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
     gene            6263..7399
                     /locus_tag="ACFNML_000485"
     CDS             6263..7399
                     /locus_tag="ACFNML_000485"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFC6391921.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADRHLCAPFDNQTALEELAKCAAVTTEFENVNADAMRFLAKHTNVSPSGDCVA
                     IAQNRIQEKSWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADIQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTIDACAADQFRQQVRIMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            7446..7928
                     /locus_tag="ACFNML_000486"
     CDS             7446..7928
                     /locus_tag="ACFNML_000486"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6391922.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8034..9089
                     /locus_tag="ACFNML_000487"
     CDS             8034..9089
                     /locus_tag="ACFNML_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6391923.1"
                     /translation="MKTYAPALYTAALLTACSPAADSNHPSGQNAPANTESDGKNITL
                     LNASYDVARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGSSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKATNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9095..9280
                     /locus_tag="ACFNML_000488"
     CDS             9095..9280
                     /locus_tag="ACFNML_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391924.1"
                     /translation="MPSEPPFRRHSTHQNRKTAPKIIITRHNGKPDTKPNTAAKMPSE
                     AVGATILIWGALHQRGA"
     gene            9671..10276
                     /locus_tag="ACFNML_000489"
     CDS             9671..10276
                     /locus_tag="ACFNML_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HFC6391925.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
     gene            10339..10701
                     /gene="ssrA"
                     /locus_tag="ACFNML_000490"
     tmRNA           10339..10701
                     /gene="ssrA"
                     /locus_tag="ACFNML_000490"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11020..11205)
                     /locus_tag="ACFNML_000491"
                     /pseudo
     CDS             complement(11020..11205)
                     /locus_tag="ACFNML_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
     gene            complement(11221..11820)
                     /locus_tag="ACFNML_000492"
                     /pseudo
     CDS             complement(11221..11820)
                     /locus_tag="ACFNML_000492"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(11774..12184)
                     /locus_tag="ACFNML_000493"
     CDS             complement(11774..12184)
                     /locus_tag="ACFNML_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HFC6391926.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKTQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLKAEMVALKKPDALIHGKEMRQKERNSSQG"
     gene            complement(12181..12513)
                     /locus_tag="ACFNML_000494"
     CDS             complement(12181..12513)
                     /locus_tag="ACFNML_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391927.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGAMVGGYALIKRGIAVVPQLAEVKKS
                     ANQEGGRQ"
     gene            complement(12625..13128)
                     /locus_tag="ACFNML_000495"
     CDS             complement(12625..13128)
                     /locus_tag="ACFNML_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HFC6391928.1"
                     /translation="MSDEFNQFINRVLSHEGGYVNHPKDPGGETNWGITKRTAMANGF
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAARMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRLNAERLVFYTKLGTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            complement(13218..13700)
                     /locus_tag="ACFNML_000496"
     CDS             complement(13218..13700)
                     /locus_tag="ACFNML_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391929.1"
                     /translation="MKKYLPLLRFLLIACISAFLAWKGQPFVHGNEKAVDLIINVFAI
                     LAGFLIAIMTLFSDMRFDEDANWRQIQIREGVQEQRYIKHSLLFYTYLAVLVCVFIVI
                     LLAHKEEYKNGPAIFWLERSYLFLACISIFYSVFLPGNLIKSRKEEFKKLMEKKKPKI
                     "
     gene            complement(13697..14608)
                     /locus_tag="ACFNML_000497"
     CDS             complement(13697..14608)
                     /locus_tag="ACFNML_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391930.1"
                     /translation="MFDLEVALRRVLSAAPTVQDTQIEQYNQLTQIMHRTLDPKDVKG
                     NSIGGLLIHIGSGTKDEHIRTMSNRPVQQDDHGGTQAPPSGYSFLRKEAFLYIVGHHV
                     IFCGHGFLSASTVASYLSLLSNKLRENNPDIVQFNIEFKAVGNCDKLSLIQQHGVKSI
                     LLDVSAYQLSRNRLYQDSRSTIAKALGKVGSVFTSELSDEELEAQSEIHINLEVLLNG
                     NSRASIEAQSLMQEQAEEIIDDETVNQGFSITTQQGEVIQPSDVKLSKSVRIGRYDEA
                     NSLLPDSAFTAISEYFLELQSRNLTEQ"
     gene            complement(14898..15143)
                     /locus_tag="ACFNML_000498"
     CDS             complement(14898..15143)
                     /locus_tag="ACFNML_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391931.1"
                     /translation="MFDFEQQIKWGERAEEIVKEAATQNNIEIPEPLASALAKAVKVH
                     YLSQAGVFSLVEAYADTVNPTEKEVDYQAIGKELFEK"
     gene            complement(15172..15549)
                     /locus_tag="ACFNML_000499"
     CDS             complement(15172..15549)
                     /locus_tag="ACFNML_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6391932.1"
                     /translation="MISKSLFGNRLKEKRKFLGLTQAQAAEKAGIERETWGKYERGVF
                     MPSGDVLLSFLNMGIDVSSLFAAEQGIRPSENSEISKEESELLGYYRQASDNGKFVIL
                     SVAKGAEKKAAENTQVSATHKTA"
     gene            15720..15842
                     /locus_tag="ACFNML_000500"
     CDS             15720..15842
                     /locus_tag="ACFNML_000500"
                     /inference="COORDINATES: protein motif:HMM:TIGR04111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391933.1"
                     /translation="MAQWARENGFKPRDVYLVVGGQRKGNYGKGHEIAKKLGLK"
     gene            15854..16144
                     /locus_tag="ACFNML_000501"
     CDS             15854..16144
                     /locus_tag="ACFNML_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFC6391934.1"
                     /translation="MAESKRVQRLLRVFITLDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            16189..17064
                     /locus_tag="ACFNML_000502"
     CDS             16189..17064
                     /locus_tag="ACFNML_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HFC6391935.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELAEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHGISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            17057..17401
                     /locus_tag="ACFNML_000503"
                     /pseudo
     CDS             17057..17401
                     /locus_tag="ACFNML_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            17376..17948
                     /locus_tag="ACFNML_000504"
     CDS             17376..17948
                     /locus_tag="ACFNML_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFC6391936.1"
                     /translation="MGKPGKSSRPSALLSARWQDIDLTARLWHIPAADMKTKHPFTVP
                     LSDWAVEILRELHTQTGDNIHLFPGIRPRRKPAPHPDHISIKFAHNAIRRLGYDGSTP
                     GKSKHTMHGFRHLFTNISLTAGKDTLTTDLALGHISRAALQRAGFSSLHHYLTADSYR
                     LQERRELAEWYSRRHRQAYEAAAQNTCNKT"
     gene            18028..18231
                     /locus_tag="ACFNML_000505"
     CDS             18028..18231
                     /locus_tag="ACFNML_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391937.1"
                     /translation="MNIIEQEMKHSSLRKVDAEIAKIIADAHKINAESVKIAQESRWY
                     PMMAATGLVTAIAAVLALIFKFA"
     gene            18278..18559
                     /locus_tag="ACFNML_000506"
     CDS             18278..18559
                     /locus_tag="ACFNML_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391938.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            18571..18783
                     /locus_tag="ACFNML_000507"
     CDS             18571..18783
                     /locus_tag="ACFNML_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFC6391939.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
BASE COUNT         5272 a         5129 c         4248 g         4402 t
ORIGIN      
        1 tcgtcattcc cataaaaaac agaaaaccaa gtgagaataa caattcgttg taaacaaata
       61 actatttgtt aatttttatt aatatatgta aaatcccccc ccccccccga aagcttaaga
      121 atataattgt aagcgtaacg attatttacg ttatgttacc atatccgact acaatccaaa
      181 ttttggagat tttaactatg aaaaaaacag tttttacatg tgccatgatt gccctgaccg
      241 gtactgccgc cgctgcacaa gagcttcaaa ccgctaatga gtttaccgtc cacaccgacc
      301 tctcttccat ttcttcaact cgtgctttcc tgaaagaaaa acacaaagct gccaaacaca
      361 tcagcgtgcg tgctgatatt ccttttgatg ccaaccaagg catccgcttg gaagccggtt
      421 tcgggcgcag caaaaaaaat attattaatt tggaaacaga tgagaacaag ctgggtaaga
      481 ctaaaaatgt aaaactgccc accggcgttc ctgaaaaccg tatcgatctt tacacaggct
      541 acacctacac ccaaacgtta agtgattctt taaatttccg tgtgggtgcc ggcttgggtt
      601 ttgaatcttc aaaagacagc attaaaacca ccaagcatac gcttcacagc agccgtcagt
      661 cgtggtcagc caaagttcac gcggatttgc tttcccaact gggtaacggc tggtatatca
      721 acccttggtc tgaagtgaaa tttgacctca attcccgcta taaattaaac accggcgtta
      781 ccagtctcaa aaaagacatc aatcaaaaaa ccaacggctg gggctttgga ttgggtgcaa
      841 atattggtaa aaaactgggc gaatccgcca gcatcgaggc ggggccgttc tacaaacaac
      901 gcacttacaa agaatccggc gagtttagtg taacaaccaa gagtagcgac gtatcgctca
      961 ccatcccgaa aaccagtatt cgtgaatacg gcttgcgcgt cggcataaaa ttctgatgat
     1021 ttgaaatcat cattgtcact ttaaatgcca aaccgcaaac tattttggtt tgcggttttt
     1081 acgtgaaatg aatttgaata gccgatgccg tctgaaactt ttcagacggc atttttccaa
     1141 tcggttaaaa tacgcggttt gttttttagg aacgcgccgt gcttgcctcc gccatcgact
     1201 tcatcctcca tatcgaccaa cacctgctcg cgctgtcggc gcaatacggt gtgtggattt
     1261 atgcgattct gtttttgatt gttttttgcg aaaccggcct gattgttacg cccttactgc
     1321 ccggcgattc gctgctgttt gccgccggcg ggattgccgc gctgggcggt atggatattc
     1381 atttgatggt tgcattatta agccttgccg ccatactcgg cgacgcgctc aattttaccg
     1441 tcggcaaata tttcggcggc aggctgttcg ccaaccccga ttctaaaatc ttccgacgtg
     1501 aatatctcga taaaacccac cgtttttacg aaaaacacgg cggcaaaacc atcatcattg
     1561 cccgttttat gcccatcgtc cgcaccttcg ccccctttgt cgccggtatg gggaaaatgc
     1621 actatgccaa atttatccgc tacaacatca tcggtggctt gttgtgggtt atcctgtttt
     1681 cttacgcggg ctatttcttt gccaacttcc ccgtcgtaaa aaacaatctg ggcttggtga
     1741 tgggcggcat cattattgtt tccgtcctgc cgggtgcagt tgaaattgcc cgcgccaaac
     1801 ttgcggtaaa atccaaacgg taactgttga gaatttgtat catgaatata ttatctgtag
     1861 aaaacgcttc ttttgccatc ggccacgttg ccttgctcga caaaacttct tttcaactcg
     1921 acagcggcga aaaagtcggc ttgatcggac gcaacggcgc aggcaagtct tcgtttttaa
     1981 aaatcctcgc cggtttgcaa aagctcgacg acgggcagat tattgttcaa aacaacctca
     2041 aaatcgttta tgtaccgcag gaatcctttt ttgataagga cgcaaccgta tttgataccg
     2101 ttgccgaagg tttgggcgaa attcgcgatt tattgcgccg ttatcatcat gtcagccatg
     2161 agttggaaaa tggttcgagt gaggctttgt tgaaagagct taacgaattg caacttgaaa
     2221 tcgaagcgaa ggacggctgg aagctggatg cggcagtcaa gcagactttg ggtgaacttg
     2281 gtttgccaga aaacgaaaaa atcggcaacc tctccggcgg acagaaaaag cgtgttgccc
     2341 tagcgcaggc ttgggtgcag aagcctgatg tattgctgct ggacgaaccg accaaccatt
     2401 tggacattga cgcgattatt tggctggaaa atctgcttaa agcgtttgaa ggcagcctgg
     2461 ttgtgattac ccacgaccgc cgttttttgg acaatatcgc cacgcgcatc gtcgaactcg
     2521 atcgcggtat tttgcgttcc tatcccggct cgttctccaa atacagcgag aaaaaagcgc
     2581 aggaattggc agtcgaagcg gaacataacc gcctctttga caaattccac gcacaggaag
     2641 aagcatggat acgcaaaggc atcgaagcgc gccgtacccg caacgaaggc cgcgtgcgcc
     2701 gtttggaaga gctgcgccgc cagagtgccg aacgccgcaa tgtacaaggg caggtcaact
     2761 ttaagctcga tagcggtgag aaaagcggca aaatcatcgc cgaattggaa cacgcttcgt
     2821 ttgcctatgg cgacaaagtt atcatggaca aattctccgc catcttgcag cgcggcgaca
     2881 aaattggctt aatcggtcca aacggcatcg gcaaaaccac atttttaaaa ctgattttgg
     2941 gcgaattgca gccgacctac ggcagaatcc gtatcggcag caagcaggaa gtcgcctatt
     3001 tcgaccagtt ccgcagcgcg ttgaatgaaa acgacaccgt gttttacacg ctcggacagg
     3061 gcaatgatta cgttgaagtc agcggtaaga aaaaacacgt catgagctat ttggaagatt
     3121 tcctgttcca tccagcccgt gcgcaaagcc ctgtttcatc gctctccggc ggagaacgca
     3181 accgcctttt gctggcaaaa ctctttaccc gtcctgctaa tattttggtt ttggacgagc
     3241 caacgaatga cttggacatc gacacccaag agctgctcga agacctgttg cgcgattatc
     3301 aaggcacggt attccttgtc tcgcacgacc gtatgttcct cgataatgtc atcacccaaa
     3361 gcattgtatt tgaaggaaac ggaaggttaa aagaatacat cggcggctat caggactaca
     3421 tcgacgcaaa atcacgcgaa gcgaagattc agacggaatc cgcacccaaa acagccgatg
     3481 ccgaaccggt gaaagaaaaa cccaaagcca accgcacggt caaactgtcc tacaaagaac
     3541 agcgagaact cgatgccctg cccgacgaaa tcgccgcctt ggaagccgaa caggcagaaa
     3601 tcaacgcgca gctttccgat cctgaaatct tcaaagatta cgaaaaagcc ggcgcactgc
     3661 aaagccgcgc cgaagaaatc gaaatgctgc ttttggaaaa gctggaacgc tgggagctgc
     3721 tggaaaccaa acggaacggc aatgccgtct gaaatcaaac aggaagaaaa aatgttcaaa
     3781 cgacctgaag aaatcattgt cctgatactt gccgtcctct ggattgccgg tacgtatttt
     3841 ctcgccgccc tgtttggagc ggatgcttat accgtgttaa aaatcactgc ccttaccctg
     3901 ctttggtctg ccgcgtcatt cctcctttgg caaaaaaacc tcagccggca tatctggccg
     3961 ctgctgctcg gctgcctgac tgcctgttgg tggccgtatc tgaatcaatc ggtcgcaccc
     4021 ggttctttac cttggtatgc atcatggacg ttcaaaacca tcttagccct gattcccgta
     4081 accgcaggtt atctgtttaa atggaaacaa taccggcagc gcaaaacaaa gccgtaagcc
     4141 cgtctttttc agacggcatc tccggtcaga ctgtcaacaa ttccgataaa atgctatact
     4201 cgcgcccgtt gccgcgccgt ccgaaaccgc cctttgggaa agcagaaatg atcagcaaac
     4261 aagaatacca agcccaagcc gcccaaggct acaaccgcat cccgctcgtg caagaactcc
     4321 ttgccgactt ggatacgccg ctttccctct atctcaaact tgccaaccgc ccctatacct
     4381 acctgctcga atccgttgtc ggcggcgaac gtttcggccg ctattccttt atcggcctgc
     4441 cttgcagcca ctatctcaaa gccagcggca aacacgtcga tgtttatcaa aacggcgaaa
     4501 ttgtcgagca acacgacggc aaccccctgc cctttatcga agccttccac aaccgcttca
     4561 aaacacccga aatcccaagc ctgccgcgct ttaccggcgg actggtcggc tacttcggtt
     4621 acgaaaccat ctacaatttc gaacacttcg cccaccgcct gaaaaacacc accaaagccg
     4681 acccgctcgg cacgcccgac atcttgctga tgctgtcgca agaattggcg gtgatcgaca
     4741 atttgagcgg caaaatccat ctcgtcgttt acgccgatcc gtcgcagccc gacggctacg
     4801 aacgcgcccg cgaacgcctc gaagacatcc gcacccagtt gcgccaaagc tgcgccatcc
     4861 cgctctcgct cggcagcaaa cacaccgaag ccgtcagcga attcggcgaa gagccgttca
     4921 aagcctgcgt taacaaaatc aaagactaca tcttcgcagg cgactgcatg caggttgtcc
     4981 ccagccagcg catgagcatg gaattcaccg acagcccgct cgccctctac cgcgccctgc
     5041 gtacgctcaa cccttcgcct tatctctttt actacgattt cggcgatttc cacatcgtcg
     5101 gctcctcgcc cgaaatcctc gtccgccgcg aacgcgacga tgtcatcgtc cgccccatcg
     5161 ccggcacgcg cctgcgcggc aaaacccccg ccgaagacct tgccaacgaa caagatttgt
     5221 taagcgatgc caaagaaatc gccgaacacg tcatgctcat cgatttagga cgcaacgacg
     5281 tcggccgcat cagtaaaacc ggcgaagtca aagttaccga caaaatggtg attgaaaaat
     5341 actcccatgt aatgcacatc gtttccaacg tcgaaggccg tctgaaagac ggcatgacca
     5401 acatggacat cctcgccgct accttccctg ccggcacact ctccggcgca cccaaagtcc
     5461 gcgccatgga aatcatcgaa gaagtcgaac cgagcaaacg cggcatctac ggcggtgccg
     5521 tcggcgtatg gggtttcaac aacgacatgg atttggcaat cgccatccgc accgccgtag
     5581 taaaaaacaa cacgctatac gtccaaagcg gcgcgggcgt ggttgccgac tccgatccgg
     5641 cctccgaatg gcaggaaaca caaaacaaag cccgagccgt tatacgcgcc gcgcagatgg
     5701 tgcaggaagg gttggataaa tgacttatga aaaactttat gagcttcgcc aaacactcag
     5761 accgactacc aaagacggtc tctatacaga taatgaaaaa aaatcgagaa atcctaacgg
     5821 ttagatggag cggcaacaca gaaaatccta aaaatttctc agctgttgct ataggaatta
     5881 atccaagtaa agcaaatgat gaaagaagcg acaaaaccct tacccaactt gcacgtttct
     5941 tggatatgta cggttttacc aacttcaaaa tgttaaatat tttttccagt tactctacac
     6001 agcaaacagg aattagggca aacacccaaa ccgatttttc caaatttaaa ggatgtttgg
     6061 aagatgctga tatgattatt cttgcatggg gtacagaccg cagtgcttat aaggatgaaa
     6121 aaaacagaat actggaattt ctaaaagcag aaaaatttat ggaaaaagta ttctgtattt
     6181 ctgaaactgg taattccagc gatacaagac atccgtcaag aattagttat agctatcaac
     6241 ttgtacaatt tgaagagtca gcatgaaaaa catatctctt tctccgcccg ccatgctcgg
     6301 cattcttggc ggcggacaat taggcagaat gtttactgtt gctgccaaaa ccatgggcta
     6361 caaagtcacc gttctcgacc ccgatccgga cgcgccggca gcggaatttg ccgaccgcca
     6421 tttgtgtgcg ccgtttgaca accaaaccgc tttggaagaa ttggcaaaat gtgcggctgt
     6481 tacgaccgag ttcgaaaacg tcaatgccga tgcgatgcgt tttctcgcca aacataccaa
     6541 tgtttccccc agcggcgact gcgttgccat cgcgcaaaac cgcattcagg aaaagtcatg
     6601 gatacgcaaa gcaggcctgc aaaccgcgcc gtatcaagca atttgcaaag ccgaagacat
     6661 cactgaagaa agcatacaat ttctgcccgg catcctgaaa accgctacat tgggctatga
     6721 cggcaaaggc caaatccgcg tcaaaacggt ggatgaactc aaagccgcgt ttgccgaaca
     6781 ccgcggcgtg gattgtgttt tggaaaaaat ggtggacttg cgcggcgaaa tttccgttat
     6841 cgtatgccgt ctgaacaatg acaacgtgca aactttcgat cctgccgaaa acattcacga
     6901 aaacggtatc ctcgcctact ccatcgtccc agcccgactg agtgccgaca ttcagcaaca
     6961 ggcgcgacaa atggcgcagc gtttggcgga cgaattggat tatgtcggcg tattggcggt
     7021 agaaatgttt gttgtcggtg acacacatga attggtcgtc aacgaaatcg cgccgcgtcc
     7081 gcacaactcc ggccaccata cgatagatgc ctgcgcggca gaccagttcc ggcaacaggt
     7141 acgcattatg tgcaacctgc cgcctgccga taccaagttg ctttcttcat gctgtatggc
     7201 gaatattttg ggcgacgttt ggcaggaaga cggcggcgaa ccggattggt ttcccctgca
     7261 aagccggccg gacgcgcacc tgcaccttta cggtaaaaaa accgcgcaca aagggcggaa
     7321 aatgggacac tttaccgttt taaccaccga ttcggacacc gcatttcaag aagcaaaaaa
     7381 actgcatcag tccctataat ccgaccaacg gcacagatgc cgtctgaagc cgccgacata
     7441 ctgccatgaa cctgctgaca ctgttacgcc ccgccaccgt gcaagactgc aaagacatct
     7501 tcaaagtcca cctgcactcc gtccaataca cctgcatcct cagctacaac gaacacgccc
     7561 taaaagtatg ggaaggcttg ttaaacacag aaagctacct gccgaccatt tcagatcccg
     7621 acaaagcact ttgggttgcc gaatacaaag gcaacataca gggatttttc caaatcgact
     7681 gccaagaagc acaactcgat gcgctttacg tccaccccct gttccacaac ctcggactgg
     7741 gcactgccct gcttcatcag gcggaaacca tcgcccacaa atccggtttg agctttctga
     7801 aactctacgc atcgctcaac tccgtcccgt tttaccttct caaccgctac gaatcgctcg
     7861 gttcggcagt attgcagctt gaccccagca tcaaaatcaa atgcgaactg atgcggaaac
     7921 acctctgatc cgtccttata accaaaactg ataatcttca aatcaaacat tatttccaca
     7981 agcgcagaca gccgtataaa gttgccccat ctaccaaccg acaggacaag catatgaaaa
     8041 cctacgcacc ggcactctat accgcagccc tgctcaccgc ctgcagcccc gcagccgatt
     8101 caaaccatcc gtccggacaa aatgccccgg ccaataccga atccgacgga aaaaacatta
     8161 ccctgctcaa cgcctcatac gatgtggcac gggattttta caaagaatac aaccccttat
     8221 ttatcaaaac ataccaatcc gaacaccccg gcacatccgt cagcatccaa cagtcccacg
     8281 gcagctccag caaacaggca ttatccgtag ccaacggcct tcaagccgat gtcgtaacca
     8341 tgaaccaatc ctccgacatc gacctgctcg aaaaaaaagg actggtagaa aaaggctggc
     8401 aacaagccct ccccgaccac gccgcgccct acaccagcac tatggttttc cttgtccgaa
     8461 aaaacaaccc caaacagatc cgcgattgga acgaccttgc caaagacggc gttaacatcg
     8521 tcatcgccaa tcccaaaacc tcgggcaacg gacgctacgc cttcctcggc gcatacggtt
     8581 acggtctgaa agccaccaac ggcaacgaac aggaagccca aaaactcgtc gcatccatcc
     8641 tcaaaaacac ccccgttttt gaaaacggcg gacgcgccgc caccaccacc ttcacacaac
     8701 gcaacatcgg cgacgtactc attacttttg aaaacgaagc caactacgtc agcaaaaaac
     8761 tgacccaagg acagtttgaa atcgtctatc cgagctacac catttccgcc gaaagccccg
     8821 tcgccgtcgt caacagcatc gtcgcgaaaa aaggcacgca aaaaaccgcc cgcgcctatc
     8881 tcgaatacct ttggagcgaa ccggcgcaag aactcgccgc ctcactctac ctgcgccccc
     8941 gcaaccccga agtattggca agacacaaag ccgacttccc agacttagac acattctctc
     9001 ccgaagaaaa attcggcgga tgggacaaca tcatgaaaac ctacttcgcc gacggcggca
     9061 tattcgaccg cctgaccgca caaaaatgac cgctatgccg tctgaaccgc ctttcagacg
     9121 gcattccaca catcaaaaca gaaaaaccgc gcccaaaatc atcatcacaa gacacaacgg
     9181 caaaccggat acaaaaccca acaccgccgc caaaatgccg tctgaagccg tcggcgcaac
     9241 catccttatc tggggcgcgc ttcatcaaag gggcgcataa ctttccttaa ccatcccttt
     9301 tgacagccaa ataaacagtg cttttgcctg tcagaaacaa aaccaatatt ttgcagttcg
     9361 tatcggaaca tttataaaac atcagatttc cgtgtttcgc gtttccggca attcaagatt
     9421 ggcaaaacac gtcagcctgc ttcgcaaggc aggtttttaa aactttatca ggcgttcggg
     9481 catatcggtt cgaccggtcc gcatttccgg caatcaacac tgccgacggt tgccgcattt
     9541 ctttttgata tacaatcaca ctttcaaaaa acatacgcag gcaacaaaat catcctcccc
     9601 cgattggcaa acattccggc aaattaaaac cccgcccgaa acagccatcg gacaaataac
     9661 aggaaaaaat atgaataccg acttccaaaa catcctcaaa caactgggca aacaagccca
     9721 aaaagaagcg gcagaaaaac aggcagccga aaaaaacaag caaaaacaag aacaggattt
     9781 cgatttttca caagcagtcg gacaagtctc cccattaaaa aaccggcagc aatattatgc
     9841 gccgcccgac aaaaccccca tcaaagtccg tcccaaagac aaccgcgccg acgaagaaaa
     9901 ctacttctac atcggcagca catacaacga cccgcccgcc agcttcagca aaaacgggca
     9961 gggaaaaaac gacatccaac gcctgaaaaa cggatactat cccgtcgtta ccgatgtcga
    10021 cctgcacggc tacacacagg aagaagccca aaaagtcctc aacgaattca tcgcattcac
    10081 ccaaaaacgc ggcgtatgcg gcgaaatcat ccacggcagc ggattaggtt ccaaaggcta
    10141 caaacccgtt ctgaaaaata tgacccgaaa ctggctaatg caacaccccg acgtactcgc
    10201 ctacgccgaa ccccgggaag gcaacgacgg ctgcgtccgc atcctgctca aacgcaaatt
    10261 aaggcagcaa gactgacaaa tttctccgac agacttggca gcaccgcaca atatccgtat
    10321 aatttgcaaa ccatttctgg gggcgacctt ggtttcgacg ggggttgcga agcagatgcg
    10381 ggcataccgg ggtctcagat tcccgtaaaa cactgaattc aaatagtcgc aaacgacgaa
    10441 acttacgctt tagccgctta aggctagccg ttgcagcagt cggtcaatgg gctgtgtggc
    10501 gaaagccacc gcaacgtcat cttacattga ctggtttcct accgggttac ttggcaggaa
    10561 atgagattta aggtaactgg tttccaaaag gcctgttggt cggcatgatg gaaataagat
    10621 tttcaaatag acacaactaa gtatgtagaa cgctttgtag aggactttcg gacgggggtt
    10681 cgattccccc cgcctccacc ataccctatt tccagcagta tcctgcaaca caaaaaagcc
    10741 ttgaacttca acagtttcaa ggcttttttg ttgctttgaa tatgctggcg tatgcttgag
    10801 ttttcgccat ttatggggta acttttgggg taacttttca cgaattaccc caaaaaacgc
    10861 cttaaaaatg caaaagtcat cacgcatttt tttaaaaagg ttacccaaaa cttaaatgta
    10921 atcctttatt tataagcttg cagctttcca aacaagttac ccccaaacgg ataaaaaaat
    10981 gccgaagccg aactgacccc aaaaagtcgg acagtttaat caagcggctt tcagggactg
    11041 aattctgtac tgaacaaggc tcagcccttt taatttcaac ttgattctat cgttgttgta
    11101 tagtggatta aatttaaacc agtacggcgt tgcctcgcct tgccgtacta tttgtactgt
    11161 ctgcggcttc atcgccttgt cctgatttaa atttaatcca ctataaaaaa acatctgata
    11221 ttgccaacct tgatccgaat gcagtatcgg cttttcagac gatcccggtt tctccggcgc
    11281 acctttcagt atctcgtcgg ccaaatcgaa agtcgggcgg gtttgaatac ggtaactgac
    11341 gatttccccg ttaaacaaat ccataatcgg agaaaggtat atcttttctc cgcctacatt
    11401 gaactcggta acgtccgtta cccatttctc attcggcttt tctgcatgga aacagcgtcg
    11461 caggatattc ggcgcaattt tgccgacttc tcctttgaac gagcggtatt tgcgccgccg
    11521 tatcactgcc ttcagccccg tcttcgccat cagacggctg actttcttgt gattgaccgg
    11581 tgttcctgca tgaaggatgg ctgctgcaat cctccggtag ccgtatcttc ccttatgtcg
    11641 tcgataaata tcatggatat gctgtttcaa atcggcatat ttgtcttctg ccgattggac
    11701 ggccaattgg taataaaagg tgccgcgtgg cagtccgaca atctccaaca gcagttcaac
    11761 ggatggcatt gccttaaccc tgcgacgagt tgcgttcttt ctgccgcatt tctttcccat
    11821 ggattaaggc atcgggcttt tttagggcaa ccatttccgc tttaaggcaa gccaattccg
    11881 caagcagttc ttccttggtt ttcagatagc cggctttttt cgtttgcggc ggatactgtt
    11941 ttttcacggg ctttcttcct tgtttttggg aaaagtcgta ccaccgctgc ctttcgtcct
    12001 gcgttttttt aagctgcaag gcatattgtt ccgccgcaac catagaagaa tgccgatagg
    12061 tggcaatcag ggcggtttgt tccgcttcgg ccttgtcggc ggcgtatcgg taaccgcacg
    12121 tccacacaag caaaagcaca aatgcccaaa gcagccgttt tttggctcgg acaaattcaa
    12181 tcattgccgc cccccttcct gatttgcgga ttttttgact tccgccaact gcggtacgac
    12241 ggcgatgccg cgtttgatta atgcataccc gccgaccatc gcgccgtatg cccaccaaag
    12301 ccattcgggc gcgtccgccg cctgcacgaa cttccaagtc atggcggctg cggcgacgtt
    12361 tgcccaaagt ttggtatggc tgattttccc tgtggccgga ttagagacca agccgcccag
    12421 ccatcggaaa aaggcggtta tttgtgacgc ttttttttgg ctgcgcgttt cgcggcggct
    12481 accccgctct tgcggtgggc ttgcgtccaa catccctgag tcggcatggc gtagcgaatg
    12541 cctaggtcgc tgtggctgaa atcgggctgc atggctgcgg tcattagggc gatgagtgac
    12601 ttttttgaca tggtttgtct ccctttaatc agtgttatct gcagacgcgt gaatcaggtt
    12661 ttgcgccaca cggcgtaccc agcccttgcc gaaagaggtg aatgtaccga gcttggtata
    12721 aaagaccaga cgctcggcgt tgagccgcaa taaaaggtcg ttttcgggga gggaattgat
    12781 ggctttgagg ctgatttcgc cgatgattcc atcgtccgcc acgcctgcgg cgcgttgcag
    12841 catacgggcg gcattgccgt aaccgtggtt gacgcaggca tcaaaaaatt ggaacgcgac
    12901 cgcttccggc atttggtcgg ctcggtaacg ctcccaaaac gcctttcggt aaatgccgat
    12961 agcctgttcg cgggtcatgg cacgcatgga accgttaaag ccgtttgcca ttgcggtacg
    13021 cttagtgatg ccccaattgg tttcgccgcc ggggtctttg ggatgattga cgtaaccgcc
    13081 ctcgtgggag aggacgcggt tgatgaattg gttgaattcg tctgacatgg aaaaatccct
    13141 gtattgaatt aaaaatcaat acagggattt tagaaaaggt cgtctgaatg ggattttaaa
    13201 gggggttaaa actcgttcta gattttgggt ttcttctttt ccattaattt tttaaactct
    13261 tcttttcggc ttttaattaa attcccaggt aagaatacag aataaaaaat ggaaatacag
    13321 gctaaaaata aatagctccg ttcaagccaa aaaatggctg gtccattttt gtattcttct
    13381 ttatgagcca acaagatgac aataaaaaca caaactaata cggcaagata tgtgtaaaaa
    13441 agtaaggaat gttttatata ccgttgttct tgtacgcctt cacgaatttg aatttgtcgc
    13501 caatttgcat cttcatcaaa cctcatatca ctaaacaatg tcataatggc aattaaaaaa
    13561 ccggctaaga ttgcaaatac gttaataatt aaatcaacgg ccttctcgtt cccatgaaca
    13621 aaaggttgcc ctttccatgc taaaaatgct gatatacaag caatcaacaa aaaacgtagt
    13681 aagggcagat attttttcat tgttcagtta agtttctgct ttggagttct aggaaatatt
    13741 cactgattgc tgtaaatgcc gagtcaggga gtaacgagtt ggcttcatcg tatcgtccta
    13801 ttctaacaga tttagacagt tttacatcag agggctgaat cacttcacct tgttgcgtgg
    13861 taatggaaaa tccttgatta accgtttcat catcaataat ttcctctgcc tgctcctgca
    13921 tcagcgattg cgcttcaata ctggctctcg aattaccgtt tagcaagact tctaaattga
    13981 tatgaatttc ggattgtgct tccaattcct catcactcaa ttcgcttgta aatacactac
    14041 caactttccc taatgcttta gcaatggttg aacggctatc ttgatacagt ctgtttctgg
    14101 ataattgata tgccgaaaca tctaaaagaa tactttttac cccatgttgt tgtatcaatg
    14161 acaacttgtc gcaattacct actgctttga attcaatatt aaattgaaca atatccggat
    14221 tgttctctct caatttattg ctaagtaaac ttaaataaga agcaaccgtt gatgctgaca
    14281 aaaaaccatg tccgcaaaaa attacatggt gtccaacaat atacaaaaaa gcctctttac
    14341 gcaaaaagga atatccactt ggaggagctt gtgttccacc atgatcatct tgctgtacag
    14401 gtctattgct catagtgcgg atatgttcat cttttgtacc gctgccaatg tgtatcagta
    14461 atccaccaat tgaattgcct tttacatctt taggatccaa agttctatgc atgatttggg
    14521 taagctgatt gtattgttca atttgtgtat cttgaacagt aggagcagct gacaaaaccc
    14581 ttcttaatgc tacctctaaa tcgaacaagc tatggttttg ctgcgcacgt aaataatgaa
    14641 tagttttagt agtttttttg ccatttgtac agtcctttct cttttaatat ctgtgtcgaa
    14701 aaaataaatt cagacggcat caattcaaat ctttcaacac ctgaaccacc tgcccgatga
    14761 cctgtatgtc ggggtagttt ttaatgcgtt ttaaaatgca cggccgcttc aattgcttaa
    14821 tatccgtttc agaacaccgg agcagcccga ctgtcggagt gtcctaaatg ccgtctgaaa
    14881 atttcagacg gcatttttta tttttcaaat agctctttgc cgattgcttg gtaatctact
    14941 tctttttcag ttggatttac cgtgtctgca taggcttcta ccaaagaaaa cacaccagct
    15001 tggcttagat aatgcacttt taccgcctta gctaatgccg aagccaaagg ttcgggaatt
    15061 tcgatattgt tctgggtggc tgcctcttta acgatttctt ccgctcgctc accccatttg
    15121 atttgttgtt caaagtcaaa cataagactg tctccatttt ggatttaaaa atcacgctgt
    15181 tttatgcgtg gctgaaactt gagtattttc ggctgctttt ttctctgcgc cttttgccac
    15241 actgagaatg acaaatttgc cgttatcgct ggcttggcgg taatacccta ataactctga
    15301 ctcttcctta ctaatttcag aattttcaga cggcctaatt ccctgttcag cagcaaataa
    15361 gctgctcaca tctattccca tattcaaaaa agagagcaat acatctccac taggcataaa
    15421 tacgccacgt tcgtatttgc cccatgtttc gcgctcaata cctgcttttt cagcagcttg
    15481 tgcttgggta agccctaaaa actttctttt ttctttcaaa cgattaccaa aaagagattt
    15541 agaaatcaca aaataatcct tgtaaaagag attttaaaat ctcataattc acgcattggt
    15601 aatgcactaa ttaatttcta gtgcataaat agttcactat tttatcacaa tgggaaaggg
    15661 ctatctatgg ctatactgtt gaaaaactaa aagaaaattt tgcccaaaac ggccaaacgc
    15721 tggcgcagtg ggcaagagag aacggcttta agccgcgcga tgtgtatctg gtggtcggcg
    15781 gacagcgcaa aggcaattac ggcaaggggc atgaaattgc caagaagctg ggactgaaat
    15841 aaggaggatg cggatggcag aaagtaaaag ggtacaacgg ctattgaggg tctttatcac
    15901 gcttgacgag catccgatta tcggtctgag caataaggat ttatcggtcg gactggggct
    15961 gacgccatcg caagtcagca gggatattga tgatttggtt gcctcgggat tggtcatcaa
    16021 acttgaaaac ggcaactacg cctacggcat caaaaccctg caaatcgcag agcggttcag
    16081 gcagcagcat gaacggctgc aaagcaagat tgccgaaatc ggcaagcggg tcgatgtgga
    16141 ttaacggatt ttgaaagttt ccgacgtcgg aaactttgga gataaaaaat gaatacagaa
    16201 attgaaatta tcgcaaccga caaagtaagc aatcaggcag cgatgcgcag tgtgctggtg
    16261 atggagcagt ggggtaacgg cgagacttat agcgaagagc gttgggttga acgcggtcgt
    16321 caagcagtac ggcagacgat ggaaggcatg ttcgaactgg gacgtgcgct gattatttta
    16381 aaagagcata cggaacacgg acggtttatg gaaattgtga aaagccaatt tgggctgggt
    16441 atcgcagaaa catcacgtct gatgtcagcc actcgccgct ttgcgacccc gcaaatgcag
    16501 aaagccgcgc cgaagctgat ggatttgggc aagtcgaagc tgctggaact cttggtcgaa
    16561 gaagacgtta cgctggtggg tttggccgaa ggggaagaag tcaacggcat gacctttgac
    16621 gatgtggacc gaatgacggt gcgcgagctg cgcgtcgccc tgcgcgaaag ccgcgaaaac
    16681 ctcgccgcca aagacgaagt gatgaaaacc aaaacagcca aaattgatga gctggccgag
    16741 aagctggcta agaagcaaac cgttgtcaga gagccgaagg cggaagacgt aggcagcgag
    16801 ttggcgatgc agttaacaag ccttgaggtc ggtatccgca gtcaagtgag ccgtctgaaa
    16861 gatttgttcg accaacttaa tgcgcacagt gaggcacacg ggatcagcca tcaggcaaag
    16921 atggtcggca cgctcaatca aattattttg gactgcgagc aactgcgcga aagctatgcc
    16981 ctgccgactg aagcaccgac agacaatgtg ccggaatggt tgggcggtga aacgggagaa
    17041 ggcgatgaat ccggcaatga ttgagcgtct taaggcagtc gagaatcagg cggaagcaat
    17101 ggaacgcggc gcacgctctg catatcttaa gcagcaggcg caggaattgg gcatcagcct
    17161 tgccacgcta taccgcaagc tggaggcggt cagcgtcaag ccgacgcgca aacggcgcag
    17221 cgatgcgggc aagacggagc ttaaaccgga agaagccaaa ttgatttcgg cggttttggt
    17281 ggaggcgatg aggcgcaacg gcaagcggtt gatgtcggtg cggcaggcgg tggaaatgct
    17341 gcgcgccaac ggaaaaatcg aggcggcgcg gattgatggg gaaaccgggg aagtcatccc
    17401 gtccctccgc actgctttcc gcccgctggc aagacatcga cctcaccgcc cggttatggc
    17461 acatccccgc cgccgacatg aagaccaaac acccctttac cgtcccactg tcggactggg
    17521 cggtagaaat actccgagag ctgcacacac agaccggcga caacatccac cttttccccg
    17581 gcatccgccc acggcgcaaa cccgcccccc atcccgacca catcagcatc aaattcgccc
    17641 acaacgccat ccgacgactg ggctatgacg gcagcacccc cggcaaatcc aaacacacaa
    17701 tgcacggatt ccgccacctc ttcaccaaca tcagcctgac cgccggaaaa gacaccctca
    17761 caacagacct cgccctcggc cacatcagcc gcgccgccct ccaacgcgcc ggcttctcaa
    17821 gcctgcacca ctacctgacc gccgacagct accgcctcca agagcggcgc gaactcgccg
    17881 aatggtacag ccgccgccac cgccaagcat acgaagccgc cgcccaaaac acttgcaata
    17941 aaacctaaaa ggtatcaata tatacacatg gcagacatcc taccatccga caagcggcgg
    18001 caaccgccaa aaacgaaagg aagtacgatg aacataatcg aacaagaaat gaaacactcc
    18061 agtctgagaa aagtagatgc cgaaatcgcc aaaatcattg cagacgcgca caaaatcaac
    18121 gccgaatccg taaaaatagc ccaagaatcc cgatggtatc ccatgatggc ggccaccggg
    18181 ctggttaccg ccatcgccgc cgtgttggca ctgatattca aatttgcctg accacaagcc
    18241 ccgcgagggg ctttatcccc cgaacaaccc gaacaaaatg aacagagccg cactattcaa
    18301 ccgctacccc gaatggatta tcgggcaaga cggcgcaagc cgtttcatta cccattgccg
    18361 ctatccccgc ctgattgcca aaatccacag gcagacagac agcgaatgcc cgggcgggca
    18421 ttaccgccat tccgaaaacg gcatcaccct ttacgatttt atttttttcg acggaaaacc
    18481 ggcagacgaa gcccgatttg ccgccgtcct taccgaaacc tgccgccggg cagtcaaaaa
    18541 aatatgcgaa acaaaataaa ccgcaacgac atggaactcg gctacacgcc ttacaacctt
    18601 cgcaccctgc gcaaccgctg caaactgaca caggccgagt tggcgcaaat cgtcggggtg
    18661 aagcactaca tacaggtagg ccgctgggaa gccgaacccg ataccgaaac gcggcgggcc
    18721 gatatgccgc tggaaaaatg gcggcagttc ctcgattgga cagaaaaaac aaacgccgtc
    18781 tgaaaagttc agacggcatt ttttaccccc aaaaacctaa aaaccacaac caagccccac
    18841 accgcttgcc cccgacctgc cgccgcccca aaataaactc actctgagac ctttgcaaaa
    18901 ttcctttccc tcccgacagc cgaaacccaa acacaggttt tcagctgttt tcgccccaaa
    18961 tacctcctaa ttttacccaa ataccccctt aatcctcccc ggatacccga taatcaggca
    19021 tccgggctgc cttttaggcg gcagcgggcg c
//
LOCUS       DAXHTR010000020        19039 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.020, whole genome shotgun sequence.
ACCESSION   DAXHTR010000020 DAXHTR010000000
VERSION     DAXHTR010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19039
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.020"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            1..110
                     /locus_tag="ACFNML_000508"
                     /pseudo
     CDS             1..110
                     /locus_tag="ACFNML_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(587..1018)
                     /locus_tag="ACFNML_000509"
     CDS             complement(587..1018)
                     /locus_tag="ACFNML_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391940.1"
                     /translation="MMFFQIACNSGNSYISLLKSMRFYIDNKECDYIFFRKAVNISDD
                     FIQSGFITPEGLITKNSNPKLFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLLNDDA
                     YIEFAYSEDFYVLPEIKIDKRTLKSLNFYIDFGVKYIINKI"
     gene            complement(1092..1535)
                     /locus_tag="ACFNML_000510"
     CDS             complement(1092..1535)
                     /locus_tag="ACFNML_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391941.1"
                     /translation="MKKQITAAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1578..2447)
                     /locus_tag="ACFNML_000511"
     CDS             complement(1578..2447)
                     /locus_tag="ACFNML_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HFC6391942.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLETSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNSKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLINPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            2611..3534
                     /locus_tag="ACFNML_000512"
     CDS             2611..3534
                     /locus_tag="ACFNML_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6391943.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHTLFEPGKVMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALALTRTDLPYPLDVVSRLVRGEKFFTTQEVEGV
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSGFCL"
     gene            complement(3612..4253)
                     /locus_tag="ACFNML_000513"
     CDS             complement(3612..4253)
                     /locus_tag="ACFNML_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6391944.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVSARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGNSEGTHDYGHGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTDRPEPSAVPNVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(4308..5099)
                     /locus_tag="ACFNML_000514"
     CDS             complement(4308..5099)
                     /locus_tag="ACFNML_000514"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFC6391945.1"
                     /translation="MARHPYRRLRPAKSGFPEVGISEEGNIRSLHLGSDTVQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFESEKFEIIEADGAEYIKVFRHNTDIILVDGFDGEQIIDTLVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFYL"
     gene            5265..5340
                     /locus_tag="ACFNML_000515"
     tRNA            5265..5340
                     /locus_tag="ACFNML_000515"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5298..5300,aa:Thr,seq:tgt)
     gene            5482..6273
                     /gene="panB"
                     /locus_tag="ACFNML_000516"
     CDS             5482..6273
                     /gene="panB"
                     /locus_tag="ACFNML_000516"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFC6391946.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVDVLLVGDS
                     LGMAVQGRQSTLPVSLRDMCYHTECVARGAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTESVSCPTIGIGAGVDCDGQVLVMHDMLG
                     IFPGKTVKFVKNFMQGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            6396..7232
                     /gene="panC"
                     /locus_tag="ACFNML_000517"
     CDS             6396..7232
                     /gene="panC"
                     /locus_tag="ACFNML_000517"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFC6391947.1"
                     /translation="MQIIHTIQELRAWRKNAGTVAFVPTMGNLHEGHLALVREAKKRA
                     DNVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFLGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            7397..9235
                     /locus_tag="ACFNML_000518"
     CDS             7397..9235
                     /locus_tag="ACFNML_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391948.1"
                     /translation="MLPARFTILSVLAAALLAGQAYAAGAADAKPPKEVGKVFRKQQR
                     YSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPEVA
                     ERALEMAVSLNAFEQAEMIYQKWRQIEPIPGEAQKRAGWLRNVLRERGNQHLDGLEEV
                     LAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSVQG
                     REKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQEM
                     EIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAYGR
                     GTGEQRGRAAMTAAMIYADRRDYTKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGGRA
                     ALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPVGSNT
                     ELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLSDSKRLDEG
                     FALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGEVL
                     WALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            9375..9956
                     /locus_tag="ACFNML_000519"
     CDS             9375..9956
                     /locus_tag="ACFNML_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HFC6391949.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            9966..10811
                     /gene="ispE"
                     /locus_tag="ACFNML_000520"
     CDS             9966..10811
                     /gene="ispE"
                     /locus_tag="ACFNML_000520"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFC6391950.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGRREDGYHNIESIFCLIDLQD
                     TVYLKPRDDGKIILHNPVGGIPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCSRTTQQLIDLGATLGADVPFFIFGKNAFASGIGEKLI
                     GMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRDSAYNIYRQVSGLYEAYLAEGLSKH
                     PLLSV"
     gene            10819..10894
                     /locus_tag="ACFNML_000521"
     tRNA            10819..10894
                     /locus_tag="ACFNML_000521"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10852..10854,aa:Gln,seq:ttg)
     gene            10907..10982
                     /locus_tag="ACFNML_000522"
     tRNA            10907..10982
                     /locus_tag="ACFNML_000522"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10940..10942,aa:Gln,seq:ttg)
     gene            10991..11066
                     /locus_tag="ACFNML_000523"
     tRNA            10991..11066
                     /locus_tag="ACFNML_000523"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11024..11026,aa:Gln,seq:ttg)
     gene            11234..12217
                     /locus_tag="ACFNML_000524"
     CDS             11234..12217
                     /locus_tag="ACFNML_000524"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HFC6391951.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKNCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            12284..12856
                     /locus_tag="ACFNML_000525"
     CDS             12284..12856
                     /locus_tag="ACFNML_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HFC6391952.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTTVEVLALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(12981..14150)
                     /locus_tag="ACFNML_000526"
     CDS             complement(12981..14150)
                     /locus_tag="ACFNML_000526"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HFC6391953.1"
                     /translation="MTAHKILPVLLSIILGISHATAASPVPNRPTAHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIQSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTITEKEIVALENVEKRSRWQRLWMRLTGQ"
     gene            14298..15824
                     /gene="ilvA"
                     /locus_tag="ACFNML_000527"
     CDS             14298..15824
                     /gene="ilvA"
                     /locus_tag="ACFNML_000527"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFC6391954.1"
                     /translation="MNTPLPYSNYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDPDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTDDEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            complement(15881..16954)
                     /locus_tag="ACFNML_000528"
     CDS             complement(15881..16954)
                     /locus_tag="ACFNML_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HFC6391955.1"
                     /translation="MSITIQNLNKHFGNFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGKILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKEQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTATGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(16951..17811)
                     /gene="cysW"
                     /locus_tag="ACFNML_000529"
     CDS             complement(16951..17811)
                     /gene="cysW"
                     /locus_tag="ACFNML_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HFC6391956.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAV"
     gene            complement(18063..18899)
                     /gene="cysT"
                     /locus_tag="ACFNML_000530"
     CDS             complement(18063..18899)
                     /gene="cysT"
                     /locus_tag="ACFNML_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HFC6391957.1"
                     /translation="MLPLKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     GGFWNTIAEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMQWALGRRSGA
                     KG"
BASE COUNT         4563 a         4419 c         5265 g         4792 t
ORIGIN      
        1 aaagcgcatt ttgagctgta tttaaaggag tgcgaatggc gttttaacaa cagtgagata
       61 aaagttcttg ttccatttta aaacaattag taaaatcaag tttatcctag ttatctagga
      121 cagcccctaa actttttgga agcatctcta atttattaat atcgttcata tcccctacac
      181 acagaaaaaa tcttggggaa tttcctttaa tctttttaat aaatcttcac aaagcctaaa
      241 atgatttaat tttagtttct ttttattttt ttgataaatt ctagataacc tcgcttatta
      301 ggaaaaattt caggatatat tcctttttct tctaaagaag tatagtggat taaatttaaa
      361 tcaggacaag gcgaggaagc cgcagacagt acaaatagta cggcaaggcg aggcaacgcc
      421 gtactaatcc actatatata tccattcata tagagaaaaa ttcttatacc caatataaaa
      481 caaggtcttt cattatattt tgaaaaacct agaagctttg tatctattgg tttatcaaaa
      541 ttaataatta ttgcagaaga catgtaggct aataatcttg atttatctat attttattaa
      601 ttatatattt aaccccgaag tcaatataga aatttagtga ttttaaagtt ctcttatcaa
      661 ttttaatctc agggagtaca taaaaatcct cagaataggc aaactctata tatgcatcat
      721 cattcaataa taattcaata atatttttca tttcatccga aagaaatgta accatctcat
      781 attgacattt atttttagat accatttttg aatattgatt aaataatttt ggattactat
      841 ttttagtaat taatccttcc ggagtaataa aaccactttg tataaagtca tcacttatat
      901 taacagcctt ccgaaaaaaa atataatcac actctttgtt atcaatataa aatctcatac
      961 tctttaataa tgaaatataa gaatttccag aattacaagc aatttgaaaa aacatcatta
     1021 ctcctaaaac tcataaatag taaggaatat atatatatgc atattcctta ctattttttc
     1081 acaaattatg cttatctatg cttatttcca taaaaagcat gccctcgaat tttttgactt
     1141 gagttctgga aatgagaata atttgaatgg atttccttct gtccatgtct aataacattt
     1201 tgtcttcccc aagcttggtt tgctaccgct cttgcttgtt gagatgttcg tgcatagaca
     1261 ttagctcctc ttgataaagc ttgtgctgca acacgctgcg taacccaacg ctgcgcaagg
     1321 aatctgcccg catgcaaaat tcctattgcc accaacggtg ccgcagcccc ttcagtttct
     1381 ttcatctcct tttgagaaag ctccgccaac tgcatcggcg catctgcctg cgtgtggaac
     1441 acttggtttt caaatgtctg attgtccaag ccgtttgcca ttgcgggggc aatcatagac
     1501 agcatcatta cggctgcggt gatttgtttt ttcataataa ctcctttgga ttacaaggtt
     1561 ggaaaatcaa aacctgctta aaatgtatgc tgtacgccaa atttcagttc ggaactgctt
     1621 tgccctgaaa cgttgaaacg tgcggatgcg tttaaagccg tggttttggt gaaaccgaaa
     1681 cctgtgccga aatgagcgta ggtggatgtg tttcttgcgg tttctttttt gccgtctatg
     1741 cggtcgggct gtgtgcccag ccattggatg cctccggtca ggctgattct gtcgttggcg
     1801 gcaaatgaga tgttggggtt gatcagcagg tagttgcccg atttgtagcg gatgccgtct
     1861 gaaagggttt tgctgccgtt gatacggtag gcggcggtga gggaaaggac aatcggatct
     1921 atggctttgt aggtggtggc gccgatgagc caggattttc ccgacgaggc tttgttgcgc
     1981 gatttttcgt aaaccgtgct ttcaagaaag ctgattaggg cggggttttt gtcgtcttta
     2041 aggaaagtgt ggctgatgcc gagggatacg tcggacatcc gtttgttgcg ggttttgctg
     2101 ttgccgtcga gtttgcgttc ttcgtgccac agatagctgc cgctgccgta aatgtcggta
     2161 ttcccggtca gtccgtagcg caaaccgagc gtgccaacga gcatatcggt attgctgccg
     2221 ttttcttgga tttcggtcgg aatggggata aacgaggttg cgccggtttg gatgtaaacc
     2281 ggtgcggcaa gttcggcgcg gttgttttcg ctattcaggt aggtaaggga agtttccagt
     2341 ttccatttgc ccttgtcagt cattatgtct tcaatcgtca agggcaggtc ggcataagcg
     2401 gataaaggca ggatggcggg caaggcgggc aaaaagatgc gcttcatatt tctcctattg
     2461 atattaattc ttatttactg ataataatga atatccgata atcttgtcta aataaatttt
     2521 taaatcataa aaaacaatat gtttgttttt gtcaattatg tttcgatatg ccgtctgaaa
     2581 agtttgtgaa aaacggttac aatccgccgc atgaaaaaac gcaataatcc tcttccgctg
     2641 ttgaacggtg tcaaacccag ttatttggtg ctgccgcatg aaaagcagtt ttacgggctg
     2701 ccgctgctgc attttctgtg catccgcttt ccttttgtgg gcgcggacga ttggcgcagg
     2761 cggttgaaca gcggttttgt ggtcggttcg gatggtgcgg cgttggacga acatactttg
     2821 ttcgagccgg gtaaggtgat gttttattat cgggaaacca gcagtgagga tgagccgcgt
     2881 attccgtttg aagaaaagat tttgcatatt gatgagcatt tgattgtggt ggacaagccg
     2941 cattttctgc ccgtcatccc cagcgggcgt tttttaaggg aaacgctgct cacacgtctg
     3001 cgcctgcgtc ccgaattgca gcatttgaat gttgaagaca ttacgccgct gcaccgcttg
     3061 gacaaggata cggcaggcgt gatgttgttg tcgcacaatc ctgccacgcg cggggcttat
     3121 cagacgatgt ttcaaaacaa aacggtatgg aaaacgtatg aggcgcttgc gctgacaagg
     3181 acggatttgc cgtatccgct cgatgtggtt tcgcgtttgg taaggggtga aaaatttttt
     3241 acaacgcaag aggtggaagg cgtgccaaac gcacatacga cggtcgaact gattgaaaac
     3301 aggggggaat tcagccttta ccgccttacg ccgcataccg gcaagaaaca ccagttgcgc
     3361 gtgcatatga tgggcttggg tatgccgctg ttgaatgacg cgctctatcc cgtgccgtct
     3421 gaagcgggca gcgaggatta tcggaaacct ttgaagctat tggcaaaaaa gattgcgttt
     3481 gcagatcctt tgtcgggtcg ggaaagggtg ttttgcagcg gattttgcct ataatgagag
     3541 ggaacgcaaa cccgtatcgg cggatgccac cgatacgggt tttacattat cgatgccgtc
     3601 tgaaaggggc tttatccgtt caggcatacg ggcgcgaagc ggaggatggt gtcgcgcaag
     3661 acgatgagtt tgccgtgaaa gaaatgcgtg gaaccggcga tggtaatgac gggcaaatct
     3721 tgcggttccg cccatttcag ggctttcccg atttcgacga cttcgtcttc cgcaccgtgt
     3781 atcatcaggg ttttggcaac gttgggaacg gcggacggtt ccggacggtc ggtatagtgg
     3841 cagactgccg cgccgatgag cagtaacagg tcgggtatgc gcgcttgtgc ggcaaatgtg
     3901 gcgacatagc cgccgaagga gaagccggat aaggcaaatt cgggggcttc gggatgttgg
     3961 gcgcgggcat agtcgatgac ggcgaggcag tcttgcgttt cgccgtgtcc gtaatcgtgt
     4021 gtgccttcgc tattgcctac gccgcgaagg ttgggcaggt agcagtggaa gccgagtttg
     4081 cttaaggctt tggcggcagt ttggatgact ttgttggtgt ttgtcccgcc ttgtaggggg
     4141 ttgggatgat tgatgacggc aacaccgcgt gccgatactt gttcggacgg gatatggatg
     4201 gtttctaaaa tgccggcagg accggatatg tgtatggttt cgggtttcag cataatgatt
     4261 tcctcttgat tttccgcatg ttccaatgcg gtcatgaaga tagttgctta aaggtagaaa
     4321 tgcttgccgt tgttggggtt ggacgctttc aggccggcaa gcatgcggtg gaagtccaat
     4381 ccgtatgcgt tgctcagttt gtcggcacgt tttttgagtt tgtctatgtt ttgctctttg
     4441 gggctgcttt ggaaggccat taccgcgaca ttgccgtggc tttcggcagg aagttccaat
     4501 acgcgccctt caaaaacgct caacaaccgt tcgatgaagc gttgatagcg tttgtcgcca
     4561 ctccaccagt tggtaacgaa tatgccgtct gaagagagtg cgtttcggca gtctcggaag
     4621 aacggttctt caaccagcgt atcgatgatt tgttcgccgt cgaatccgtc caccaagatg
     4681 atatcagtgt tgtggcggaa gactttgatg tactctgcac catccgcttc aataatttca
     4741 aatttctcac tctcgaaagg caactcaaac aggttgcggg caatggcgat aacctgcggg
     4801 ttgatatcta cggcagtttg acgcgtgtcg ggcaggtagg tgtctatcca gcgcgcaaac
     4861 gagccgccgc ccaagccgat ttgggtgata tgctgcggaa gcgcgtcggt aaacagcagc
     4921 catcccatca tcgcgcggct gtaagagagt accagctcgg acgggtggtc gaggttcatt
     4981 gagctttgaa cggtgtcgct gcccaagtga agcgaacgga tattgccttc ttcggaaatg
     5041 ccgacttcgg gaaagccgga ttttgcagga cgcaggcggc ggtagggatg tcttgccatc
     5101 ggtgtgaacg gtcggtttga aaggcggata ttttatcaga atgccgatgc ttgtcctgct
     5161 tcaaattaat ttcttttcaa ataaattact tattcggatt tgccggggct ttcggataaa
     5221 ttccttgcca aagtgtgaag ttgcctgcat aattcgcctt ctttgccggt atagctcagt
     5281 tggtagagca cctgacttgt aatcaggggg tcccgagttc gactcttggt gccggcacca
     5341 gatttgacag tccgattgtg taaaacagtc gggctgtttt gcatttgcgg cacggtcggt
     5401 ggcagggctg cgggcatttc actataatag cccgtttcaa actgaaaagg ccgtctgaaa
     5461 agggcggggt aacaatatct gatgattact gtgaacacac tgcaaaaaat gaaggcggcg
     5521 ggcgagaaaa tcgctatgct gaccgcttac gaatccagtt ttgccgcgct gatggacgat
     5581 gccggcgtgg atgtgctgct ggttggggat tctttgggaa tggcggttca agggcggcag
     5641 tcgacgctgc ccgtcagcct gcgcgatatg tgttatcaca ccgaatgtgt agcacgcggt
     5701 gcaaaaaatg cgatgattgt cagcgatttg ccgtttggtg catatcagca gagtaaggag
     5761 caggcgtttg ccgccgccgc cgaactgatg gctgccggcg cgcatatggt caaactcgaa
     5821 ggcggtgtgt ggatggcgga aacgaccgaa tttctgcaaa tgcgcggtat tccggtctgt
     5881 gcccacatcg gtctgacccc gcaatccgtg tttgccttcg gcggatacaa ggttcagggg
     5941 cgcggcggca aggcgcaggc gttgcttaac gatgccaagg cgcatgatga tgcgggggcg
     6001 gcggtcgtgc tgatggagtg tgttcctgcg gaactggcaa aaaaggtaac tgaatccgtt
     6061 tcctgtccga ccatcggcat cggcgcgggt gtcgattgcg acgggcaggt tttggtgatg
     6121 cacgatatgc tcggcatttt cccgggtaag acggtgaaat tcgtcaaaaa ctttatgcag
     6181 gggcaaagca gcattcaggc ggcggttcgg gcgtatgttg ccgaagtcaa agccaaaacc
     6241 ttccctgctg cggaacatat ttttgcagac tgaacaaggt ttgctgccga tgccgtctga
     6301 aagccgtttc agacggcatt ttgttttgcc gtgcgcggcg cgtataatcg gcgcgttttg
     6361 tcgggcagga agcccgaagg ataaggatta ccgttatgca aatcatacat accattcaag
     6421 aactgcgcgc gtggcgtaaa aatgcgggaa cggtggcatt tgtgccgacc atgggcaatc
     6481 tgcacgaagg gcatcttgcc ctcgttcgcg aagccaaaaa acgcgccgac aacgttgttg
     6541 tcagcatatt cgtcaaccgc ctgcaattcg gtcagggcga ggatttcgac aaatatccgc
     6601 gtactttgca acaggatgcg gacaagctgg aagccgaggg tgttgccgtt gttttcgcgc
     6661 ccgatgagaa agaactctat ccgaacgtgg aacagcgtta caacgtcgaa ccgcccaatc
     6721 tgcaaaatga gttgtgcggc aagtttcgtc cgggacattt cctcggggtg gcgacggtgg
     6781 tgtccaagct gttcaatatt gtcgcgccgg atgttgcctg tttcggcaaa aaagactatc
     6841 agcagcttgc cgtgattaaa ggtttgaccg aggatttgaa ttttgatgtt gaaatagtgc
     6901 ctgtcgatac ggggcgcgcg gaagacggtt tggcactctc cagccgcaac cagtatttga
     6961 gtgcggcgga acgcgacgaa gcaccgcgcc tgtaccgcga attaaaggct gttgccgaat
     7021 ccttggcgca gggcagtttg gattatgcag gtttggaaaa acgtgccgtc caatccctga
     7081 cagaatacgg ctgggtggtc gattatgtcg aaatccgccg cgtcgatacg ctcgaagtgg
     7141 cgcgggcggg agataagaaa ctggtggtct tggccgccgc ccgtctgggg acgacgcgcc
     7201 tgattgacaa tttggaaata aaactccctt aaaccgcaag cgtcgggaat gccgtctgaa
     7261 gcggatttgc gtttcagacg gcatttattt ttttgaacgg ggtttcgcaa atctacaaac
     7321 gattctgctt gtgataaagt tacgcctgat tatatgccgt ctgaaagttc ggacggctgt
     7381 cggataaagg aagattatgt tacccgcccg tttcaccatt ttatctgtgc tcgcggcagc
     7441 cctgcttgcc gggcaggcgt atgccgccgg cgcggcggat gcgaagccgc cgaaggaagt
     7501 cggaaaggtt ttcagaaagc agcagcgtta cagcgaggaa gaaatcaaaa acgaacgcgc
     7561 acggcttgcg gcagtgggcg agcgggttaa tcagatattt acgttgctgg gaggggaaac
     7621 cgccttgcaa aaggggcagg cgggaacggc tctggcaacc tatatgctga tgttggaacg
     7681 cacaaaatcc cccgaagtcg ccgaacgcgc cttggaaatg gccgtgtcgc tgaacgcgtt
     7741 tgaacaggcg gaaatgattt atcagaaatg gcggcagatt gagcctatac cgggtgaggc
     7801 gcaaaaacgg gcggggtggc tgcggaacgt gctgagggaa agaggaaatc agcatctgga
     7861 cggactggaa gaagtgctgg ctcaggcgga cgaaggacag aaccgcaggg tgtttttatt
     7921 gttggcacaa gccgccgtgc aacaggacgg gttggcgcaa aaagcatcga aagcggttcg
     7981 ccgcgcggcg ttgaaatatg aacatctgcc cgaagcggcg gttgccgatg tggtgttcag
     8041 cgtacaggga cgcgaaaagg aaaaggcaat cggagctttg cagcgtttgg cgaagctcga
     8101 tacggaaata ttgcccccca ctttaatgac gttgcgtctg actgcacgca aatatcccga
     8161 aatactcgac ggctttttcg agcagacaga cacccaaaac ctttcggccg tctggcagga
     8221 aatggaaatt atgaatctgg tttccctgca caggttggat gatgcctatg cgcgtttgaa
     8281 cgtgctgttg gaacgcaatc cgaatgcaga cctgtatatt caggcagcga tattggcggc
     8341 aaaccgaaaa gaaggtgctt ccgttatcga cggctacgcc gaaaaggcat acggcagggg
     8401 gacgggggaa cagcggggca gggcggcaat gacggcggcg atgatatatg ccgacagaag
     8461 ggattacacc aaagtcaggc agtggctgaa aaaagtgtcc gcgccggaat acctgttcga
     8521 caaaggtgtg ctggcggctg cggcggctgt cgagttggac ggcggcaggg cggctttgcg
     8581 gcagatcggc agggtgcgga aacttcccga acagcagggg cggtatttta cggcagacaa
     8641 tttgtccaaa atacagatgc tcgccctgtc gaagctgccc gacaaacggg aggctttgag
     8701 ggggttggac aagattatcg aaaaaccgcc tgtcggcagc aatacagagt tacaggcaga
     8761 ggcattggta cagcggtcag ttgtttacga ccggcttggc aagcggaaaa aaatgatttc
     8821 agatcttgaa agggcgttca ggcttgcacc cgataacgct cagattatga ataatctggg
     8881 ctacagcctg ctttccgatt ccaaacgttt ggacgaaggc ttcgccctgc ttcagacggc
     8941 ataccaaatc aacccggacg ataccgctgt caacgacagc ataggctggg cgtattacct
     9001 gaaaggcgac gcggaaagcg cgctgccgta tctgcggtat tcgtttgaaa acgaccccga
     9061 gcccgaagtt gccgcccatt tgggcgaagt gttgtgggca ttgggcgaac gcgatcaggc
     9121 ggttgacgta tggacgcagg cggcacacct tacgggagac aagaaaatat ggcgggaaac
     9181 gctcaaacgt cacggcatcg cattgcccca accttcccgc aaaccccgga aataatgcag
     9241 gtccatcctt tcagacggca taaggtttgc cgggaagctg cggcattcgg gcaaacggca
     9301 cgcagttcgc acgcgttttg cacggcacac cgaacccatc ggccggcagg atggcatccg
     9361 ttaaggaaat tctgatgaaa cacactgtat ccgcatcggt catcctgctt ttgaccgctt
     9421 gcgcgcaatt acctcaaaat aacgaaaacc tgtggcagcc gtccgaacac acccgcagtt
     9481 tcacggcaga agggcggttg gcagtgaaag cggaagggaa aggttcgtat gcaaatttcg
     9541 attggacata ccaaccgccc gtggaaacca tcaatatcaa tacccctttg ggcagtacgc
     9601 tcgggcagtt gtgtcaagac agggacggcg cattggcagt ggacggcaaa ggaaatgtct
     9661 atcaggcgga aagtgcggaa gaattgagca ggcagctggt cggtttcaaa ctgccaatcc
     9721 aatatctgca tatctgggca gatggcaggc gtgtggcggg cgcgccttac cgcatcctgc
     9781 cggacggcat attggaacaa tacggttgga ctgtcggcag aaccgccgac agtggggggc
     9841 aagtccgaac gttgcaactg aataacggaa atttgaacat caggctggtt ttcaccgaaa
     9901 tcggtatgcc gtctgaaacc gaaaccccgg aacgctgtgc ggcgcgcacg agataaggcg
     9961 gacagatgaa tattgcggac ggacggcagg cgtttcccgc acctgcaaaa ctgaatctcg
    10021 atttgaggat taccggcagg cgggaagacg gttatcacaa tatcgaaagc atattctgcc
    10081 tgatagattt gcaggatacc gtatatttga aaccgaggga cgacggcaaa atcatcctgc
    10141 acaatcctgt cggcggaata ccgcaggaag ccgatttgag ctaccgtgcc gcatcgttgc
    10201 tgcaaaaata tgcgcgcaac cccgccggcg tggaaatatg gctggacaaa aaaatcccga
    10261 caggggcggg tttgggcggc ggaagctcgg atgcggcaac cgttttgctg gtgttgaacc
    10321 gttggtggca gtgttcccga acgacgcagc agctgattga cttgggcgca actttgggtg
    10381 cggacgtacc attttttatt ttcggaaaaa atgcttttgc aagcgggata ggtgaaaaac
    10441 tgataggaat ggatattccg aaacagtggt atgtcatcgt caaaccgccc gtccacgttt
    10501 ccactgcaaa aattttcaca cacgaaggct tgacacgaaa ttccgcctca agcataatgc
    10561 cgactttcca aaacctgcaa ccgtttagaa atgatatgca ggcagttgta tttaaagaat
    10621 accctgaagt ttggaaagcc tattccgaat tgtcccgata tggatttgcc ttaatgacag
    10681 gttctggtgc gtgtgtattc acggcgtgtc aagataggga tagcgcatac aatatatacc
    10741 gacaagtttc aggtttgtac gaggcatatt tggcagaggg tctttcaaaa catcctttgt
    10801 tgtccgtata aacattgttg gggagtcgtc aagcggttaa gacactggat tttgattcca
    10861 gcatgcgaag gttcgaatcc ttcctcccca gccaagccaa acgagttggg gagtcgtcaa
    10921 gcggttaaga cactggattt tgattccagc atgcgaaggt tcgaatcctt cctccccagc
    10981 cagccctttt tggggagtcg tcaagcggtt aagacactgg attttgattc cagcatgcga
    11041 aggttcgaat ccttcctccc cagccagata aaagcgtgta agcctgctta cacgcttttt
    11101 atttcataga aataaaaata ttgaaatgcc tttgtttgtg tcggatgttg caggtataat
    11161 gtcgggcttg gtacaagcag agggaagcat tgtgttttct gagcggaagt taaacataaa
    11221 atcaggtgag aatatggctg cgtacgacag tttgatggta tttacaggca atgccaatcc
    11281 cgaattggca caacgtgttg tcaggcattt ggacatttct ttgggcaatg cttccgtatc
    11341 caagttttca gacggcgaag ttgccgtcga actgttggaa aacgtacgcg ggcgcgatgt
    11401 tttcatcctt cagccgacct gtgcgccgac caatgacaac ctgatggaaa tcctgacgat
    11461 ggcggatgca ctgaagcgtg cttcggcagg tcgtattacc acagccattc cgtatttcgg
    11521 ctatgcgcgc caagaccgcc gtccgcgttc cgtccgcgtt ccgatttctg ccaaactggt
    11581 ggcaaatatg ctgtattcgg cagggatcga ccgtgttttg actgtcgatt tgcatgccga
    11641 ccagattcaa ggtttcttcg atattccagt ggacaatatt tatgccaccc cgattctgtt
    11701 gaacgacatc aaacaacagc ggattgaaaa tctgaccgtc gtcagcccgg acatcggcgg
    11761 tgtcgtccgc gcccgcgccg tggcaaaatc cctgaatgcc gacttggcaa tcatcgacaa
    11821 acgccgcccg aaagccaatg tggcggaagt catgaacatc atcggcgata ttcaaggcag
    11881 aacctgtctg attgtggacg atatgattga cactgcaaat acgctgtgca aagccgccgt
    11941 cgctctgaaa gagcgggggg cggaacgtgt cttggcatat gccagccacg ccgtattctc
    12001 cggagaggcg gtcagccgta tcgcctcatc cgaaatcgac caggtggtcg taaccgatac
    12061 cattcctttg tctgaagcgg ctaaaaactg cgaccgtatc cgtcaggtaa cgattgccgg
    12121 tctgttggcc gaaaccgtcc gccgcatcag caatgaagaa tccgtctcat atcttttcaa
    12181 tgaagaagtg atgacaggca gtatgttgct gccataagcc cgaagccgtc ttaagctggt
    12241 cgcggccgat gacggcgatt ttacctaact tggagtattt aacatgactt atgaaattca
    12301 agcctctgtt cgtgaagcac aaggcactgg tgcgagccgc cgcctgcgtc gcgaaggcca
    12361 aatccccggc attctgtacg gtgaaggtca agagcctgtt gcaatcgctg tggatcacaa
    12421 aaccgtattc tacgcattgg aaaaagaatc tttccatacc gcgttgatta aattgtctct
    12481 gaacggcgaa accaaagacg ttatcgtccg tgatttccaa atgcacccgt tccgccgcga
    12541 agttcaacac atcgacttcc aagctgtgaa agccgatcaa cttgtacgca tccgtgttcc
    12601 cctgcacatc gttaacgctg aaaattccca agcggtcaaa ctgcaaggcg gccgtgtatc
    12661 tctgttgaac acgactgttg aagtgcttgc attgcctgcc aatatccctg ctttcttgga
    12721 tttggattgt gccgaagtgg ttgccggcga cattctgcat ctgtccgaca tcaaactgcc
    12781 tgaaggtgta gaaagcgttt ccttgaaacg taacgaaaat ctggctgttg ccaccgttac
    12841 cggtaaaaaa cgctaattga tttcagcagc agggcggcgc gtatgcaata cgtaccgccc
    12901 tgttgtttta tgccgtctga accgtgtttc agacggcatt tctttatttg ttggaaaaac
    12961 gggatatttg aaacggcaga ttactgtccc gtcagacgca tccaaagcct ttgccaccgg
    13021 cttctttttt ctacattttc cagtgcgaca atttcttttt cggtaatggt gtatccgttt
    13081 tgcctgattt tgatttttcc taaaatctgt ccctttttta ccggggcggg aatcggctgt
    13141 atggtttcta gaatttgttc tgccattttc gcttccttat gtggcagagt gatgtaggct
    13201 tctttgagga agcctgcgcg gacggttttt ttgctgcctc cggaaatttg gatttgggca
    13261 acggttttgc ctttcggata tattttgggc gtatcgaagg cctgcaatgc ccagttcagc
    13321 agcttgctgt tgtctgatgc gcgtgtttcc gccgattccg aacccaatgt gatgacaagg
    13381 atgtgcctgc cgttgccgga gtatgacacg gcaaggttgt agccgccgct ttctgtgtgt
    13441 ccggctttca gaccgtttac attgttgtcc ctatataaaa ggatattgcg gttgttttgc
    13501 tctatatttt tgaatttgaa agatttgatg gaaaacagcg ggtaatattc cggaaagtcg
    13561 cgcatcaatg cttcagacag ctgggcgagg tctttggcgg tggaaacctg tccttctcta
    13621 ctcaagcctg tcgggttttt gaatacagtg ttcttcatgc ccaagcgtcg ggcttctttg
    13681 ttcatttgtt gcacaaaatt ttcaatcgag ccgttgccca gccggccggc aagggttagg
    13741 gcggcatcgt ttgcggatag ggcaatcatg ccttttaaga gtttgtcggt gctgaccgta
    13801 tcgccgggac gtacaaacat tctgcttcct tctgaagccc atgcggattc gggtattttt
    13861 aagttttctt cagattggat atttcccgat ttcatgtttt tgaaaaccaa atatgcggtc
    13921 atcagttggg ttagtgccgc cggttcgaca ggggtattga tgtttttggc ggataaagtc
    13981 tgtctgcttt gaaggtcgat aacgatgtgt gccgctgtga gggtttcggg tgtttggagc
    14041 gtgggggcgg cgtgtgccgt cggtctgttg ggcacgggcg atgcagccgt tgcgtgagaa
    14101 atgcctaaga tgatggaaag caggacgggc aggattttat gtgctgtcat gaaatattct
    14161 aattgtgtgc gtgtttcagt ctgccgatta tacgcttagg gtgtctgatc gggcggattt
    14221 ttcttgcttt cgcgccgtct tgggcgtatg gttttgggtt ttgcgatttt aataaaccga
    14281 ttatcccata ttgaattatg aacacgcccc ttccttattc caattacctc atccgcatcc
    14341 tgacggcatc tgtctatgat gtcgcggtcg aaacgccttt ggaaccggca cgcagccttt
    14401 ctgtacgttt gaaaaacaac atccttttga aacgcgaaga tttgcagccg gttttttcgt
    14461 tcaaaatacg cggcgcgtac aacaaaatgt ccaagttgcc gaaagatgcg ctcgcttgcg
    14521 gcgtgattgc ggcaagcgcg ggcaatcatg ctcaaggcgt ggcattgtcc gcacagcgtt
    14581 tgggctgccg tgccgttatc gttatgccgg agactacgcc gaaaatcaaa gtggatgcgg
    14641 ttaagagccg tggcggcgag gtggttttgc ggggcgtttc atacaacgat gcctacgatt
    14701 atgcgatgga gttggcggaa aaagaagggt taacctatat cgcgccgttt gatgatcctg
    14761 atgtgattgc gggacagggg acggtgggga tggaaattgt cagccagcat cccgatccaa
    14821 tccgcgccgt attcgtaccg ataggcggtg gcggtttggc ggcgggcgtg gcggcattta
    14881 tcaagcaggt ccgtcccgaa atcaaagtta tcggcgttca gaccaacgat tcctgctgta
    14941 tgaagcagtc ggtcgaagcg ggtgaaatcg tccatttgaa agatgtcggg ctgttttcag
    15001 acggcactgc ggtcaaagtc gtcggaaacg aaaccttccg cctctgcaaa gaacttttgg
    15061 atgaaatcat tacagtcgat accgatgcgg tttgcggcgc ggtcaaggat attttcgatg
    15121 acacgcgcag cattaccgag ccggcgggcg cgttggcgtt ggcgggtctg aaagcctata
    15181 tcgcccgaga aggcgcggaa aaccaaaccc tgattgccgt taccagcggt gcgaatatga
    15241 attttcaccg tttgcgccat gtgtcggagc gtagtgaatt gggcgagggc aacgaaggta
    15301 tttttgccgt taccatcccc gaagaacgcg gcagcttcct taagtttgtc aatatattgg
    15361 gaaataggaa tattaccgag ttcaactacc gctacggaga cgatgaaaaa gcgcatatct
    15421 ttgtcggact tcaagcggca ggtccgcagg atttggcggt tatcggtagc cggttggatg
    15481 aggcgggatt gcccaatgtc gacctgactg atgatgagat tgccaaaatc catatccgct
    15541 atatggtcgg agggcggacg gacaaagtag aaaacgagcg tctggtcagt tttgagtttc
    15601 cggagcgtcc gggggcgttg gcgcgttttt tgaaccatat gcagggcggt tggaacatta
    15661 cgctcttcca ttaccgcaac cacggtgcgg attacgggcg gattttggtc ggtatcgatg
    15721 tgccgccgca cgatgccgcc gcatttgacg gtttcttgga aagtctggga tacagctatc
    15781 acgaggaaac gcaaaatgcc gcgtacaagc tgtttcttgc ctgacgcttg aaagcacaat
    15841 gccgtctgaa agcctttcag acggcattgc gctttcatgg ttaaatcgaa tattcaatca
    15901 gttcgttttg agagaagaca taaacctgtt tcggaatcag cttcaattct ttgccttcgg
    15961 cgattgggta acgcgcggca tcgctgccag ccagcgtgat atgcacgtct tgtttgccgt
    16021 gtttcaccaa aatatgcgtc aatgccccga cggcgtggac tttttcgatt tcggcacaaa
    16081 tcatcggtgt ttcgtgttcg gcggcgatct gccattcgtg cgggcggata tagccggtgg
    16141 cggtttgttc ctgccatttg tattgtgcgt ccaatttcca cgcgaagccg ttataatgcc
    16201 agaagccttt ttcgatacgt ccttcaaaag cgtcggtttc gccgaggaac tcggtaacga
    16261 aggcattttc gggtttgcgg taaatagctt cggcgctgcc ggtttgttcg attttgccgt
    16321 ggttcatcac gacgatttcg tcggaaactt cgagggcttc ttcttggtcg tgcgtaacca
    16381 gaatgctggt tacacccagg ttgtgatgga tgtcgcgcag ccaggtgcgt aattctttgc
    16441 gtactttggc gtccaacgcg ccgaagggtt cgtccaaaag caggagtttc ggttcgaccg
    16501 caagcgcacg ggcgagggcg atgcgctggc gttgcccgcc ggagagttgg tgcggatagg
    16561 attttgccaa atgcgagagt tgtacgagtt tcagcaattc ttcgacttta gtgcagattt
    16621 gttctttgga cgggcgttcg gacttgggca atacggtcaa accgaaagcg acgttgtcaa
    16681 acacgttcat gtggcggaag agggcgtagt gttggaacac gaagccgact ttgcgctcgc
    16741 ggacgtgttt ggcggttacg tcttgcccat caaacagaat cttgccgccg tcggcgtttt
    16801 ccagtccggc gataatgcgt aaaagcgtgg ttttgccgca gccggacggg ccgagcaggg
    16861 aaacgagttt gccggtgggg acgttgaggt tgatgttttt cagcgcgtga aaattgccga
    16921 agtgtttgtt taagttttgg atggtgatac tcatactgcg ttcctttcgg cggcggcgag
    16981 ttttttgtct tgtaatttgg tgatgatgtt ctgcaccgcc agcgtcgcca gtgccaaaag
    17041 tgccaatacg ccggagaggg cgaatgcgcc ggtgaagttg tattcgttgt agaagatttc
    17101 gaccaaaagc gggacggtgt tggtttcgcc gcgtatgtgt cccgatacca cgctgaccgc
    17161 gccgaactcg cccatcgcgc gggcgttggt gaggatgatg ccgtagagta acgcccattt
    17221 gatgttgggc agggtaacgc gccaaaacat ctgccagccg cttgcgccga gtatcaatgc
    17281 cgcctgttct tcgctgtcgc cctgagcctg catcagcggg atgatttcgc gcgcgacaaa
    17341 ggggaaggta acgaacagcg tcgccagaat aatgccgggg atggcgaaga taatctgtat
    17401 gccttgcgct tcgagccagc cacccaatgc cgtgtgcgcg ccgaacaata agacgaacat
    17461 taaaccggcc accacgggcg atacggaaaa cggcaaatcg agcagggtag tcagcaactg
    17521 cttgccgcga aaatcaaaac gggtcagcag ccacgccatc gccacgccca agacggcatt
    17581 gacggggacg acaatcagcg cggtaatcag cgtcaatttg atggcagacc acgcttcggg
    17641 atcgtttaag gatttcaggt acaaatccca accgcctttt aaggcttcgt aaaatacggc
    17701 gacgagcggc acgaccagca tcagcagcag aaagcccagc gcggcggcaa tcagcaacac
    17761 gcgcagccga cgcggttcgg tcaggttggg attggcggaa tagggtttca tggcggtggt
    17821 gttctctggt tggttttaaa atgtttggag gtcgtctgaa aaactgtgtt tttgggagcg
    17881 gattcgtttt cagacaacct tttattgatt aagggaataa aaacgtctag ctaccgtcat
    17941 tcccgcgcag gcgggaatcc accacagggc aacaggaaaa cagaaaataa ataaggcagc
    18001 cgaaattcac caatggattc ccgcctgcgc gggaatgacg gtaacaggta tttcagacga
    18061 cctcaaccct tcgcgcccga acgcctgccc aacgcccact gcatcacgtt cagcgcaaac
    18121 agaatcacaa acgaaaccag cagcataaac aacgccaccg ccgacgcgcc ctgtacgtcg
    18181 aactgttcca gcttgcccgt aataatcagc ggcaggattt cagaaaccat cggaatattg
    18241 cccgcgataa aaatcaccga gccgtattcc cccgttgccc gcgcgaacat cattcccgcg
    18301 ccagtcaaga gtgccggcgt gatttccggc agcagcacgc ggcggaacgt cgtaaagcgg
    18361 tttgcgccca aagtcgccgc cgcttcctca tattcgcccg ataattcctc caacaccggc
    18421 tgcacggcgc ggacgataaa gggcaggctg acgacgacca gcgcaatcca aatgccgacg
    18481 ggcgtaaacg cgattttgat gcccaaaggc tcgaaaaaac ggcctatcca accgttgggc
    18541 gcatacaggg tcgccaacgc gatacccgta accgccgtcg gcagcgcaaa cggcaaatcg
    18601 accagcgcgt tcgccagacc cttgcccggg aattcataac gcaccaatac ccacgccacc
    18661 agcgtgccga acacgacatt ggtcagcatc gcataaaacg acatccgcaa actcagccag
    18721 acggccgcca agacattggg ttcggcaatc gtgttccaaa agccgcccca gccgatttcc
    18781 gccgccttcg ccgccatcat cgcaaacggc aagaccacaa gcagcgacag gcacaatacg
    18841 gtcagaccaa ggctgagttt gaagccgggc agtacgccgg gcgttttgag cggtaacata
    18901 aaacaatgct gaaaataagg aaaaggaagg actactttaa cgatgccgtc cgaaaaacgg
    18961 aaagaatgga aagtttggtg caaagacgaa tttgttataa agcggttggc agtttctcaa
    19021 gcgggcgcga tgttttaaa
//
LOCUS       DAXHTR010000021        19020 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.021, whole genome shotgun sequence.
ACCESSION   DAXHTR010000021 DAXHTR010000000
VERSION     DAXHTR010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.021"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..817)
                     /gene="hslO"
                     /locus_tag="ACFNML_000531"
     CDS             complement(<1..817)
                     /gene="hslO"
                     /locus_tag="ACFNML_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFC6391958.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTADELAELDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHS"
     gene            1062..1817
                     /locus_tag="ACFNML_000532"
     CDS             1062..1817
                     /locus_tag="ACFNML_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6391959.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            1836..2054
                     /locus_tag="ACFNML_000533"
     CDS             1836..2054
                     /locus_tag="ACFNML_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391960.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            2199..2543
                     /locus_tag="ACFNML_000534"
     CDS             2199..2543
                     /locus_tag="ACFNML_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFC6391961.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            2543..3235
                     /locus_tag="ACFNML_000535"
     CDS             2543..3235
                     /locus_tag="ACFNML_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HFC6391962.1"
                     /translation="MNEILRQPSVLLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILDIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            3302..4522
                     /gene="argJ"
                     /locus_tag="ACFNML_000536"
     CDS             3302..4522
                     /gene="argJ"
                     /locus_tag="ACFNML_000536"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HFC6391963.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADETF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            4582..5984
                     /locus_tag="ACFNML_000537"
                     /pseudo
     CDS             4582..5984
                     /locus_tag="ACFNML_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
     gene            6173..7564
                     /gene="hrpA"
                     /locus_tag="ACFNML_000538"
     CDS             6173..7564
                     /gene="hrpA"
                     /locus_tag="ACFNML_000538"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFC6391964.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     RRLAALPKPEFDNTLPVHEKLEEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSRHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHIEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLKLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            7665..8792
                     /locus_tag="ACFNML_000539"
     CDS             7665..8792
                     /locus_tag="ACFNML_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HFC6391965.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            8970..10546
                     /gene="lpt3"
                     /locus_tag="ACFNML_000540"
                     /pseudo
     CDS             8970..10546
                     /gene="lpt3"
                     /locus_tag="ACFNML_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
     gene            10648..13551
                     /locus_tag="ACFNML_000541"
     CDS             10648..13551
                     /locus_tag="ACFNML_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HFC6391966.1"
                     /translation="MDARSNPVNVSDGLQNSSGHIGTNTRYRLTKLGEQMARLPIDPK
                     IARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHERFTDKQSDFLAYL
                     NIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQTAIEMGLTTKEAA
                     FRRPPEIRQLTSSESQGDQDLAAKLKQKQLDKKQHRAQIRAAKEAGYEQIHRALLTGL
                     IANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVETTRLYARDVAVIQ
                     PEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRPVPYGKVAPEEAR
                     EIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDDEALFAFYHERLP
                     NFYTADAVSDDLHPTNPQQTAPSPVGEGWGEGKTVAAQTNFSATAANPLPQEGEQSAA
                     ASALSDDPQPQKQPASQKGRLKPLPLADIRTFQAWLKTAERENPRLLFLSRDDLMQHA
                     AAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTLTLPLTVLNRISPAALEWLVPG
                     MLREKIQLLIKALPKQIRRICVPVPDFITKFLESNPDRQAAIIPQLAHFIAKSAGDMR
                     IFEQIDQDAWAVQELPEHCYLNLRIIDDGGQELAGGRKLHELQQQLGQAAATTFRDNT
                     QEFERDNVTAWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALRLCDTIEAAEQAH
                     RQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLTQAVCDRAFIGED
                     ELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAAYAELNGKLGKHPLTHLLRLRL
                     QTLLAPSFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREADIQELEQMWQEK
                     TDSLVKQGLPISDGLAGFKWMIEELRVSLFAQELKTPYPVSVKRLMKEWERLN"
     gene            13614..15221
                     /locus_tag="ACFNML_000542"
     CDS             13614..15221
                     /locus_tag="ACFNML_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6391967.1"
                     /translation="MKSENNYERIVQNSKKFQHSQKNEMQKRITDLLQQLTNGIHERD
                     QIMAVSLLGAIAGHNTFLFGPPGTAKSLISRRLACAFENPQYFEYLMNRFSTPEEVFG
                     PVSIKALKEDRYLRKIEGYLPTADFAFLDEIWKSSPAILNNLLTIINEHIFKNGDERF
                     NVPLKALIAASNEIPPENQGLEALYDRFIIRLLVPPIEQEENFNRLLDSKPSSDKPEI
                     SEDLRISYHELAKWREQLHKVRLSTDTLLIIKYIREELAAQFDKLGVYVSDRRWQRAA
                     VLLKASAFCNGRKETNHSDVILLKYCLWTTPENRESVEQIVMNAIKSCGFDTGIDLAE
                     LDREKESLDKEINKELYHSQDVYETVEMSGKKYFPVQAVFKNVHNSHANKELKIYVPY
                     SKFRSKEKFYPIDSTGNEIQEIECEFDGQGCCKLELKVRGGHWNEQYKPLKFKPNILF
                     HKGDKKSDVNERLIHSLTQSISDIRKQLIDTQKQVEKKYEKYQQYLQSPFVAQADIDV
                     AISGILDQIDKLNLRIKDCERLESLCR"
     gene            15212..16672
                     /locus_tag="ACFNML_000543"
     CDS             15212..16672
                     /locus_tag="ACFNML_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003758253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFC6391968.1"
                     /translation="MSLNRKLSEQIRWIKASLQIDYADLFKQHGSLNGQIQQRLSDWQ
                     YQAKLALLQKNPFILHEEKLKKHQQTEIFNQQSVDDYRYFCKLAGVPFEKEFWQQELT
                     QSKNSKPNKQNLPLKLLTGKWQQQLDYAKSQWYLKQLNQLRQEFIDKLKQELDTIRQL
                     AEQLEQFGFEAGLWFDNSIGNLSQQNIEEMKRWLNYLTQDKNAQQIAELLGKMRQIEQ
                     SEKIEQVKQTVYTKSPQIDVNSREEIIGLRLGKDLEYVLPSELALMADEETSTLFDLK
                     FLESKLMCFELQGMTYCDAPTEIIVEQKSQEDEKLGPMILCVDTSGSMVGLPENIAKA
                     MALFLGAKAKSENRSCFVINFSTGIETFELTSKTGISNLIAFLRQSFHGGTDAAPALR
                     HALKMMEQEIYQKADLLMISDFVMNSLPDDLLASIEIQRETGNQFNSLVIGDAFMSKR
                     LKTHFDREWIYNPNVQTIQELVQFKKDIFNKQVVNS"
     gene            complement(17026..17499)
                     /locus_tag="ACFNML_000544"
                     /pseudo
     CDS             complement(17026..17499)
                     /locus_tag="ACFNML_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            17731..17943
                     /locus_tag="ACFNML_000545"
     CDS             17731..17943
                     /locus_tag="ACFNML_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger domain-containing protein"
                     /protein_id="HFC6391969.1"
                     /translation="MPHIHPLHLFPPHIAERGLLYFQQGKVGAVQKTSTGRYRAEVCG
                     LENYWVWLKLDSDLYIKDESCDCPYV"
     gene            18127..18395
                     /locus_tag="ACFNML_000546"
                     /pseudo
     CDS             18127..18395
                     /locus_tag="ACFNML_000546"
                     /inference="COORDINATES: protein motif:HMM:NF017953.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(18485..18934)
                     /locus_tag="ACFNML_000547"
     CDS             complement(18485..18934)
                     /locus_tag="ACFNML_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6391970.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         5062 a         5260 c         4398 g         4300 t
ORIGIN      
        1 tgctgtggca gaaatcgcaa tcgacttcga tgctgccttg ttccaccacc acgccgccga
       61 cttcttcccc gcccagcatc agcagcatat cgctgacttt gccgcgcgaa caggtgcatg
      121 aaaattcaaa cgtttccggc tcgaacacgc gcggcggcgt ttcgtggaac aggcggtaca
      181 aaacatgttg cgcatccaat tctgccagct cgtccgccgt cagtgtacgc gccagcgtac
      241 tgacgtgttc ccatgcctct tcatccaaca ccgcttcagg cagacgctgc accagcagac
      301 cgcccgccgc ttcgtcgctt gcagacagga cgatgtgcgt atcgatttgt tcggaacgct
      361 tcatatagtt cgccaacatt tgcgcgatac cgccgccttc caaaggcact acgccctgcc
      421 agggttcgcc gtctttgggc tgcagcgtca gcacgaatac gccgccctcg cccaaaaggt
      481 cgccgaggct ttcgtcatcg gctatttctg cggtttcgtc ccaacgcgcg gttgcacgga
      541 cggtacggtc ggaagccgct tccgcaacca gcattttcag ccgcccccgc ccctgaacct
      601 gcacaatcag cgtgccttcg tttttgaggt tgcccgacag caacacaccc gccgccaaca
      661 actcacccaa agcgcggcgg atggcggcgg gatagttttt ctgttttaca atgtgctgcc
      721 acacgttttc cagacggacg tgcagcccgc gcacgggcat atcgtcgaag ataaagcggg
      781 tacgcacatc ggcgcggttg atggcggttt gattcatgat tttctctgac tgattgttcg
      841 gatggcggct atatggttgc ggtcggcgcg aaaacaagac ggacggcgga tgcgcttccc
      901 aaattatcaa taaattatat aaaaatcaac atattaactc aatctaacaa gccgtttttt
      961 gccaaacagc cgttttttta tatacaatca acaagatatt ttcgactgat acagcataac
     1021 atcgcacggc ggcacgatgc ctcctgcgcg gaaacaccga tatggattct tttttcaaac
     1081 cggcagtttg ggcggttttg tggctgatgt ttgccgtccg ccccgccctt gccgacgagt
     1141 tgaccaacct gctcagcagc cgcgagcaga ttctcagaca gtttgccgaa gacgaacagc
     1201 ccgttttacc catcaaccga gcccccgccc ggcgggcggg caatgccgac gaactcatcg
     1261 gcagcgcgat ggggcttaac gaacagcccg ttttacccgt caaccgagtc cccgcccggc
     1321 gggcgggcaa tgccgacgaa ctcatcggca acgcgatggg gcttaacgaa cagcccgttt
     1381 tacccgtcaa ccgagccccc gcccggcggg cgggcaatgc cgacgaactc atcggcaacg
     1441 cgatgggact tttgggtatt gcctaccgct acggcggcac atcggtttct accggttttg
     1501 actgcagcgg cttcatgcag cacatcttca aacgcgccat gggcatcaac ctgccgcgca
     1561 cgtcggcaga acaggcacgg atgggtacgc cggttgcccg aagcgaattg cagcccggag
     1621 atatggtgtt tttccgcacg ctcggcggca gccgcatttc ccatgtcgga ctttatatcg
     1681 gcaacaaccg cttcatccac gcgccgcgca cggggaaaaa tatcgaaatc accagcctga
     1741 gccacaaata ttggagcggc aaatacgcgt tcgcccgccg ggtcaagaaa aacgacccgt
     1801 cccgctttct gaactgattt cccaaggaat acgcaatgag tatgcccgaa atgcccaaat
     1861 ggtacgacga tgacggacag atcgtgtcct gtaccgaaaa ggtcaaagtg atgtccgaaa
     1921 atatggccga gctgtatcag acggcacaag acgcgtttga agacgcgctg ctgatgggtt
     1981 gcggcgaacg tcaattgcgc gattacctgc tcgcgctgat tgaaggtttg gaaaatccct
     2041 accgcaaagt ctgaacacgc cccggttgct gcggcacggt ttatccgtgc cgtttttgcg
     2101 tttgtgcgcg gcttcggctt ttcagacggc atatttgacg ttatgattaa acagttaaca
     2161 agatttatca caacgccgtc aaaagacaga cacacaacat gaacatcatc cgcgcgctcc
     2221 tcatcatcct cggctgcctc gccgccggcg aaaccgccgt tttcctagca ggcatcaaac
     2281 tgcccggcag catcgtcggc atgggcgtgc tgtttgcgct tttgcaggcg ggttgggtca
     2341 aaacgtcttg gctacaacag cttaccgacg cgctgatggc gaatctgacg ctgtttctcg
     2401 tgccgccctg cgtggcggtc atcagctatt tggatttgat tgccgacgat tggttttcga
     2461 tactggtttc cgcctccgcc agcactttgt gcgtactgct ggttacaggc aaggttcacc
     2521 gctggatacg gagcattatc tgatgaacga aatcctcagg cagcccagcg ttctgctttt
     2581 cctcacgctt gccgtgtacg cgcttgcgat tatcgtgcgc acgcgcacgg gcaatatctt
     2641 ctgcaacccc gtactcgtca gcactatcgt gctgattgcc tacctgaaaa tcctcgacat
     2701 cgattatgcg gtgtaccaca acgccgcgca attcattgat ttttggctga aacccgccgt
     2761 cgtcgtgctt gccgtgccgc tctaccaaaa ccgccgtaaa atcttcaacc agtggctgcc
     2821 cgtcatcgtt tcacagcttg cgggcagcgt tacgggcatt gttacgggga tgtattttgc
     2881 caaatggctg ggcgcggaac gcgaagtcgt cctctcgctc gcgtccaaat ctgttaccaa
     2941 ccccatcgcc attgaaatca cccgctccat cggcggcatt cccgccatta ccgccgccac
     3001 cgtcatcatt gccggtctgg tcggacagat tgccggttac aaaatgctga agaacacagt
     3061 cgtcatgccc tcgtccgtgg gtatgtcgct cggcacggct tcgcacgcga tggggattgc
     3121 cgcctcgctc gaacgcagcc gccgtatggc ggcatacgcg ggactggggc tgacgttcaa
     3181 cggcgtactg accgcgctga ttgcgccgct gctcatcccc gttttgggat tttgaacccg
     3241 tttcagacgg catttcagcc catgctgtct gaacgccgac acactcgcaa ggagaaccgt
     3301 tatggctgtc aacctgaccg aaaaaaccgc cgaacaactg cccgacatcg acggcattgc
     3361 cctctacacc gcccaagcag gcgtgaagaa gcccgggcat accgacctga cactgattgc
     3421 cgtagccgcc ggcagcaccg tcggtgcagt cttcacgacc aaccgtttct gtgccgcgcc
     3481 cgtccacatc gccaaatcgc accttttcga cgaagacggc gtgcgcgccc tcatcatcaa
     3541 cacgggcaac gccaacgcgg gtacgggcgc acagggcaga atcgatgctt tggcagtgtg
     3601 tgccgccgcc gcccggcaaa tcggctgcaa accgaaccag gtgctgccct tctccaccgg
     3661 cgtgattctc gaaccgctgc ccgcagacaa aatcatcgcc gcgctgccca aaatgcagcc
     3721 tgccttctgg aacgaagcgg cacgcgccat catgaccacc gacaccgtgc ccaaagccgc
     3781 ctcgcgcgaa ggcaaggtcg gcgacaaaca caccgtccgc gccacgggca tcgccaaagg
     3841 ctcgggcatg attcatccca atatggcgac catgctcagt ttcatcgcca ccgatgccaa
     3901 agtttcccaa cccgtcctcc aactgatgac gcaggaaatc gccgacgaaa ccttcaacac
     3961 catcaccgtt gacggcgaca ccagcaccaa cgacagcttc gtcatcatcg ccaccggcaa
     4021 aaacagccaa agcgaaatcg acaacatcgc cgacccgcgt tacgcccaac tcaaagaatt
     4081 gttgtgcagc ctcgcgctcg aactcgccca agccatcgtc cgcgacggcg aaggtgcgac
     4141 caagttcatc accgtccgcg tcgaaaacgc caaaacccgc gacgaagccc gccaagccgc
     4201 ctacgccgtg gcacgttcgc cgctggtcaa aaccgccttt ttcgcctccg accccaacct
     4261 cggcagactg ctcgccgcca tcggttatgc cggcgttgcc gacctcgata ccgacctcgt
     4321 ggaaatgtat ctcgacgata ttttggttgc cgaacacggc ggacgcgccg caagctacac
     4381 cgaagcacaa gggcaggcgg tgatgtcgaa ggccgaaatc accgtccgca tcaagctgca
     4441 tcgcggacaa gccgccgcca ccgtctatac ctgcgacctg tcgcacggat acgtttccat
     4501 caacgccgac taccgttcct gacccgacac ggcttcagac ggcatacata aaatgccgtc
     4561 tgaaccgccg gacaacatac catgacctcc acattccccc gccgcctcgc ccgcaaaatc
     4621 cgccaaaccc gccgcctgtc gcgcaaaagc atcgcctttc tgttcctttt ggcaggttcg
     4681 gcactcgtcg ccctgaccgc gctgtttttt gcccatcttg ccgattttgc gctggaactg
     4741 aacgccaaac tggttcaaca atacccgtgg ttcgcgtggg tcgcgcttcc tttgggttta
     4801 ccgcttattg cgtggctcac acgcaaattc gcccccttca ccgccggcag cggcatcccg
     4861 caggtcatcg cctcactgtc gctgccctac ggcgcacaga aaacgcggct gatccgcctc
     4921 gggcagacac tgctgaagat tccgctgacc tttttgggta tgctgttcgg cgcgtccatc
     4981 ggacgcgaag gtccgtccgt gcaggtcggc gcggcagtga tgggcgcgtg gggcgcgtgg
     5041 tgcaagaaac acggcttggc attcaaaggg atgcaggaaa acgatttgat ggcggcgggc
     5101 gcggcgggcg gtttggcagc cgcgttcaac gcgccgctgg cgggcgtgat tttcgccatt
     5161 gaggaactcg ggcgcggcat catgttgcgc tgggaacggc agattctgct cggcgtactc
     5221 gccgccggtt tcatacaggt cgccattcag ggcaacaacc cgtatttttc cggcttcaac
     5281 ggcggcgtat tggaacatat ctttctgtgg gtcgcactgt ccggcctggt ttgcggcgcg
     5341 gcgggcgggc tgttcggacg tttgctctat cgcggtgcgg cggcgtttgc accgcgcaag
     5401 atacgcggct tcatccgcaa ccgtccgctg ctgctggcgg cactgatggg gctgctgctc
     5461 gccctgctcg gcacgttcta ccaaggcaaa acctacggca ccggctacca cgaagccgcc
     5521 caagccctgc acggcatcta cgaagccccc ttcggactca ccgccgccaa atggctcgcc
     5581 accgtattca gctattgggc aggcattccg ggcggcattt tcactccctc gctgaccata
     5641 ggcgcggttt tgggcgagca tatcgccgcc atcgccgaca tatcgcaggg tgcaaacatc
     5701 atcgtcctca tctgcatggc ggcatttctg gcgggcgcga cacaatcccc gattacttcc
     5761 gccgtcgtcg tcatggaaat gacgggcgga caaagcctgc tgttttggat gctgattgcc
     5821 tgcattttcg cctcgcaggt ttcgcgccag ttttcgccgc gtccgttcta ccacgcatcg
     5881 ggaatgcgct tccgccagcg cgtgcttcaa gaaaccgccg cccaaaccgg caatgcgccc
     5941 gcaagaccgc aaacagcaaa acgggaatgc cgtctgaaaa ttaaaacgcc cccgatcaaa
     6001 cgccggcagc cgccttgatt cgaataccgt tccgccgccg cttgaaattt cagcaacaat
     6061 gccgtctgaa cgacagaatg cggttttcag acggcatttc cccatcccga tattgcctaa
     6121 acaaaaccga agcgtttgct ataattgcac tttttaccgc acacgcacgc ccatgtttcc
     6181 cgatttctcc caaaccctct ccaaagaccg ccacttcctg caatccgcct tcaaaaatcc
     6241 caacaaatac ggcggtttgt ccaaagtcga agaaaaatac cgaaaatcgc acgaaatctt
     6301 tttgaggcgt ttggccgcat tgcccaaacc cgaattcgac aacaccctgc ccgttcacga
     6361 aaaactcgaa gaaatcaaaa aaaccgttgc cgaacatcag gtaacgatta tttgcggcga
     6421 aaccggttcg ggcaagacca ctcagttgcc caagatttgt ttggaactcg ggcgtggggc
     6481 ggcggggctg atcgggcata cccagccgcg ccgtttggcc gcgcgctccg tggcagagcg
     6541 gattgccgaa gagctgaaat ccgaaatcgg cagcgcggtc ggctataaag tacgcttcac
     6601 cgaccacacc tcgcgcgatg cctgcgtcaa gctgatgacc gacggcatcc tgctggcgga
     6661 aacgcagacc gaccgttatc tcgccgccta cgacacgatt atcatcgacg aagcgcacga
     6721 gcgcagcctg aacatcgact tccttttggg ctatttgaaa caactcctgc cgcgccgccc
     6781 cgatttgaaa gtcatcatca cctcggcaac gatagacgca gaacgcttct cccgacactt
     6841 caacggcgcg cccgttttag aagtgagcgg acggacgtat cccgtcgaaa tcctctaccg
     6901 accgctgacc ggcaaagacg aagacgacgc agaagtggag ttgaccgacg cgattgtcga
     6961 tgcggcggac gaattagcgc gacacggcga aggcgatatt ttggtattcc tgccgggcga
     7021 gcgcgaaatc cgcgaagccg ccgaagccct gcgcaaatcc acgctgcgcc gcaacgacga
     7081 aatcctgccc ctgttcgcac gcctgtcgca cgccgagcag cacaaaatct tccacccctc
     7141 aggcgcgaaa cgccgcatcg tattggcaac caacgtcgcc gaaacctcgc ttaccgtgcc
     7201 gggcatcaaa tacgtcatcg acaccggcct cgcgcgtgtt aaacgctatt ccgcacgggc
     7261 gaaagtggag cagcttcata tcgaaaaaat ctcccaagcc gccgcccgcc aacgatccgg
     7321 ccgctgcgga cgcgtctccg caggcgtgtg tatccgactg ttttcagaag aagatttcaa
     7381 cagtcgcccc gaatttaccg accccgaaat cgtccgcagc aacctcgccg ccgtcatcct
     7441 gcgcatggca tcgctgaaac tcggcgacgt agcggcattc ccatttttag aaatgcccga
     7501 ttcgcggtat attaatgatg ggtttcaggt gttgctggaa ttaggggcgg tggaggccgc
     7561 ctgaaaacaa gtaggatata cacttctacc tgacaaagac caacaaacga taaaactacg
     7621 gcacatctag atgcccaacc tacaacattg cacacggtaa aaacatgaaa cacttactca
     7681 tcgattttga aaacgtccag ccgcaaaact tagacaaatt actaaccgaa aatacccata
     7741 tttggctatt tataggtgta ttacacaaaa tgttacctat tagtctggtg caatccctac
     7801 tacgtttcgg cgaacgtgtc catcttgtcc agttacaaaa aacagggaaa aacgcattgg
     7861 atttttacct gtcctattac ctcggacaaa ttaccgccac agaccccaat gcccaaatcg
     7921 gcatactctc gcgtgatgga ggatacgatg ttctggtcga acatattttg aaaaaccacc
     7981 aggcgaaggg tatcgtgcgc ctagccaata tagatgaagt acaacatcag aaaattacta
     8041 ccgaaccgcc gtcagcattg ctggaaaaca ctcctcagcc tgaaaccacc ctcaaaccac
     8101 agcaaccatt aacttcctat ttccaagcag ccctaactgc actgcgccgc cccgacgctt
     8161 tccgcccctg ccgcctgcat aacctgcgac aaaatctgcg taagcatatt ttgagtgatt
     8221 tgtttaaaga aaaaaccgat gaagaatgcg aaataaccac tgctaacgtt atcaataaac
     8281 tcaaagcaca aaacttcatc agcattgatg aacaggaaac cgtttcctac catctcagtg
     8341 ataatgattt gttacaaaga atccaacgcc atattttaag ccaacgtccc aaaacctacg
     8401 ctgattttca agccgtcgtg caaaaccgag cagatgcact tcacttaaca gtcggtacca
     8461 acgacattca atcctttgcg cgacatttgc gcgaccaaaa cctgatccgc caaaacaatg
     8521 ggaaaattga atatgcaccg tttactgaac ctaaaccaca gccaacgccc aagcagccta
     8581 aaaaaaccgc atgggaacct gatgaaatta tttggaaaaa agtgattgcc gcgttatcgt
     8641 taaagaaccg tcctaataaa accaaaactt tacgcaatac aatccaggca ctcacaaaat
     8701 ccaatgcaca agaaactgac aaactgctac aacatttaca agatacccaa gtcctacgta
     8761 ttgacggtac taaaattgtg tatatcaagt aggatgggca tttatgcctg acctacaaaa
     8821 ccaaaaacaa cctaccaccc ttaatcaact ccacaaaccc ttttcagaca acctcgtttt
     8881 ttgaaaaaca atctgtaaac agatagctgc tgaagaatac cgttgccgag ccccaaaacc
     8941 cgtcctgcaa cttttattgt gaacttccca ttatgaaaaa atcccttttc gttctctttc
     9001 tgtattcgtc cctacttact gccagcgaaa ttgcttatcg ctttgtattc ggaattgaaa
     9061 ccttaccggc tgcaaaaatg gcggaaacgt ttgcgctgac atttgtgatt gctgcgctgt
     9121 atctgtttgc gcgttataag gcgacgcgtt tgttgattgc ggtgtttttt gcgttcagca
     9181 ttattgccaa caatgtgcat tacgcggttt atcaaagctg gataacgggc attaattatt
     9241 ggctgatgct gaagagatta ccgaagtcgg cggcgcgggc gcgtcgatgt tggatcagtt
     9301 gtggctgcct gcgttgtggg gcgtgttgga agtcatgttg ttttgcagcc ttgccaagtt
     9361 ccaccgtaag acgcattttt ctgccgatat actgtttgcc ttcctaatgc tgatgatttt
     9421 cgtgcgttcg ttcgacacga aacaagagca cggtatttcg cccaaaccga catacagccg
     9481 catcaaagcc aattatttca gcttcggtta ttttgtcgga cgcgtgttgc cgtatcagtt
     9541 gtttgattta agcaggattc ccgcctttaa gcagcctgct ccaagcaaaa tcaggcaggg
     9601 cagtgttcaa aatatcgtcc tgattatggg cgaaagcgaa agcgcggcgc atttgaagct
     9661 gtttggctac ggacgcgaaa cttcgccgtt tttaacccgg ctgtcgcaag ccgattttaa
     9721 gccgattgtg aaacaaagtt attccgcagg ctttatgact gcagtgtccc tgcccagttt
     9781 tttcaatgcg ataccgcacg ccaacggctt ggaacaaatc agcggcggcg ataccaatat
     9841 gttccgcctc gccaaagagc agggctatga gacgtatttt tacagtgctc aggctgaaaa
     9901 ccaaatggca attttgaact taatcggtaa gaaatggata gaccatctga ttcaaccgac
     9961 gcagcttggc tacggcaacg gcgacaatat gcccgatgag aagctgctgc cgttgttcga
    10021 caaaatcaat ttgcagcagg gcaagcattt tatcgtgttg caccaacgtg gttcgcacgc
    10081 cccatacggc gcattgttgc agcctcaaga taaagtattc ggcgaagccg atattgtgga
    10141 taagtacgac aacaccatcc acaaaaccga ccaaatgatt caaaccgtat tcgagcagct
    10201 gcaaaagcag cctgacggca actggctgtt tgcctatacc tccgatcatg gccagtatgt
    10261 tcgccaagat atctacaatc aaggcacggt gcagcccgac agctatctcg tgccgctagt
    10321 gttgtacagc ccggataagg ccgtgcaaca ggctgccaac caggcttttg cgccttgcga
    10381 gattgccttc catcagcagc tttcaacgtt cctgattcac acgttgggct acgatatgcc
    10441 ggtttcaggt tgtcgcgaag gctcggtaac gggcaacctg attacgggtg atgcaggcag
    10501 cttgaacatt cgcgacggca aggcggaata tgtttatccg caatgagtgg cgtaaaaacc
    10561 aataaagaca aatttagatg atgtcgggga agatgcccga ccgacaagac tatgcaaaat
    10621 atgaaaaacc aagtacgcgg atcaggcatg gatgcccgat ccaatccggt caatgtttca
    10681 gacggcctgc aaaacagttc gggtcatatc ggtaccaaca cgcgttaccg cctgaccaaa
    10741 ctcggcgaac aaatggcgcg cctgcccatc gacccgaaaa tcgcgcgcat tttgctggcg
    10801 gcgaagaaac acgactgcat ggcggaaata ttggtgatag cgtccgcgct gtcgattcaa
    10861 gacccgcgcg agcggccgtt ggaagcgcgc gatgccgccg ccaaggcaca cgagcgtttt
    10921 accgacaagc aatccgactt ccttgcctat ctgaacattt gggacagctt ccagcgcgag
    10981 cgcgacaaag gtttgtccaa caagcagttg gtgcaatggt gccgtcaata tttcctgtcg
    11041 cacctgagga tgcgcgagtg gcgcgagttg caccaccagc ttgcccaaac cgcgattgaa
    11101 atgggtttga ccaccaagga agcggctttc agacggcctc ccgaaatcag gcagctcacg
    11161 tcgtctgaaa gccaaggcga ccaagatttg gcggccaaac tcaaacaaaa acaactggat
    11221 aagaagcaac accgcgccca aatccgcgcc gccaaagaag cgggctacga acaaatccac
    11281 cgcgccctgc tgaccggcct tatcgccaac gtcggcatga aatcgcccga cggcaacgac
    11341 tacaccggcg cacgcggcag ccgttttcat cttttcccgg catcagccct gttcaaagcc
    11401 aagcccaaat gggtgatggc ggcagaattg gttgaaacca cgcgccttta cgcgcgcgac
    11461 gtcgccgtta tccagcccga atggatagag caggaagcgc cgcacctcgt ccgctatcat
    11521 tatttcgagc cgcattggga acaaaaacgc ggcgaagtcg tcgccagcga acgcgtgacg
    11581 ctttacggtc tgaccgtatt gccgcgccgc cccgtgcctt acggcaaagt tgcgcccgaa
    11641 gaagcacgcg aaatctttat ccgcagcgca ttggtggcgc aggaatgcga tttgaaagcg
    11701 gatttttttg tccacaacaa aaagctgatt aaagaaatta ccgaactcga acacaaatcg
    11761 cgcaagcaag acgtattggt cgacgacgaa gccctgtttg cgttttatca cgaacggctg
    11821 cccaattttt atacggcgga cgcggtttca gacgacctgc atcctacaaa tccgcagcaa
    11881 actgccccct cccccgtggg ggagggctgg ggagagggca aaacagttgc cgcacaaacc
    11941 aacttctccg caaccgcagc aaaccctctc ccacaggagg gggaacagag tgccgcagct
    12001 tcagcacttt cagacgaccc tcaaccccaa aagcagccag catctcaaaa aggccgtctg
    12061 aaacccctcc ccctcgccga catccgcacc ttccaagcct ggctcaaaac cgccgaacgc
    12121 gagaacccgc gcctgctgtt cctcagccgc gacgatttga tgcaacacgc cgccgcacac
    12181 attaccgaag aacaattccc caaattctgg caaaccgcag acggcaaatt caaactcagc
    12241 taccgcttcg agccgcacca cccgctcgac ggcgttaccc tcaccctgcc gctcaccgtg
    12301 ctcaaccgca tcagccccgc cgccctcgaa tggctcgtgc ccggtatgtt gcgcgagaaa
    12361 atccagctgc tgattaaagc actgccgaag caaatccgcc gcatttgcgt gcccgtaccc
    12421 gatttcatta ccaaatttct cgaaagcaat cccgaccgcc aagcggccat cattccccag
    12481 ttggcgcact ttatcgccaa aagcgcaggc gatatgcgga ttttcgagca aatcgaccaa
    12541 gacgcatggg cggtgcaaga attgcccgaa cactgctatc tgaatctgcg cattatcgac
    12601 gacggcggac aagagcttgc cggcggccgc aaactgcacg aattgcaaca acagctcggc
    12661 caagccgccg ccaccacctt ccgcgacaac acccaagaat tcgagcgcga caacgttacc
    12721 gcatgggaca tcggcaccct acccgaatcc atcaaattcg cacgcggcaa acaacagctc
    12781 accggctatc tcggtctgca aaaagaaaaa gacggccgca tcgccctgcg cctgtgtgac
    12841 accatcgaag ccgccgagca ggcacaccgt caaggcgtca tcgaattgat gaagctgcaa
    12901 ttaaaagagc aggtaaaaga cctgaacaaa ggcatccaag gcttcaccca agccgccatg
    12961 ctgctcaaac acatcaacgc cgacaccctg cgcgacgacc tcacccaagc cgtctgcgac
    13021 cgcgccttta tcggcgaaga cgagctgccg cgcaacgaaa aagccttcaa agaacaaatc
    13081 aaacgcgccc gcagccgcct gcccgccgtc aaagaagctc tcagtcgtta cctgcaggaa
    13141 accgccgccg cctatgccga actgaacggc aaactcggca aacacccgct cacccatctc
    13201 ctaagactac gcctgcaaac cctgcttgcc cccagcttcg ccacccgcac cccgtgggca
    13261 caatggccgc gcctccccat ctatctcaaa gccatgaccc tgcgcctcga aaaatacagc
    13321 agcaaccccg cccgcgacgc agcccgcgaa gccgatatcc aagagctgga acaaatgtgg
    13381 caggaaaaaa ccgatagctt ggtgaaacaa ggattgccca tttcagacgg ccttgcaggg
    13441 tttaaatgga tgattgaaga attgagggtg tcgctgtttg cgcaggaatt gaagacaccg
    13501 tatccggtgt cggtgaagcg gctgatgaag gagtgggaga ggttgaatta agtagtgata
    13561 ttatccaata catttacagg aaaatttcta acttacataa aaggtacaac tcaatgaaaa
    13621 gcgaaaataa ttatgaacgt attgttcaaa attctaaaaa attccaacac tctcagaaga
    13681 atgagatgca gaaacgcata acagatttgc tgcaacaact cacaaatggc attcatgaac
    13741 gcgaccaaat tatggcggtt tccctacttg gtgcgattgc cggacataat actttcctat
    13801 ttggaccgcc gggaaccgcg aaaagtctga tttcccgccg tttggcatgt gcatttgaaa
    13861 atccgcaata ctttgaatac ttgatgaacc gtttcagcac acctgaagaa gtattcggac
    13921 ctgtatctat taaggcattg aaagaagacc gatatctccg aaaaatcgaa ggctatttgc
    13981 caactgccga ttttgcattt ttggatgaaa tttggaaatc cagtcctgca attttgaata
    14041 acctgctgac cattatcaac gagcatattt ttaaaaacgg cgatgagagg tttaatgtgc
    14101 cacttaaagc cctgattgcg gcttccaacg aaattccacc tgaaaatcaa ggtttagagg
    14161 cactgtatga ccgctttatt atccgtctgc ttgtgccgcc gattgaacaa gaagagaact
    14221 tcaaccgttt gctggatagc aaaccaagca gcgacaaacc tgaaatcagt gaagatttac
    14281 ggattagtta tcatgagctt gccaaatggc gtgaacaact ccacaaagtc aggctaagta
    14341 cagatacatt actgattatt aagtatatca gagaagaatt agcagcccag tttgataagt
    14401 taggcgttta tgtatccgac cgacgttggc aacgtgcagc cgtattatta aaggcttctg
    14461 ctttttgcaa tggacgcaaa gaaaccaatc atagcgatgt gatcttgctg aaatattgtc
    14521 tgtggacaac acctgaaaat cgagagtcag tggaacaaat tgtgatgaat gccattaaat
    14581 cctgtgggtt tgatacaggt attgatctgg ctgaattaga tagagaaaaa gagagtttgg
    14641 ataaggaaat aaataaggaa ctttatcatt cacaagatgt ttatgagacg gttgaaatga
    14701 gtggaaaaaa atattttcca gtacaggctg ttttcaaaaa cgtacataat agtcatgcta
    14761 ataaagaact taagatttat gttccatata gcaaatttcg tagtaaagaa aaattttatc
    14821 caatagatag tacaggaaat gaaattcaag aaattgaatg tgaatttgat gggcaaggtt
    14881 gctgtaaatt ggaattgaaa gtgcggggtg gtcactggaa tgagcaatat aagccattaa
    14941 aatttaaacc caatatctta tttcacaaag gagataaaaa atcagatgtt aacgaacgtc
    15001 taattcattc actaacccaa tctatatctg atattcgcaa acaattgatt gatacacaga
    15061 aacaagttga gaaaaaatac gaaaaatacc agcagtacct gcaaagcccc tttgttgccc
    15121 aagcagatat tgatgttgct atatcaggta ttctagacca aatcgataaa ctcaatttgc
    15181 ggattaaaga ctgcgaacgt ttggagtctt tatgtcgtta aacagaaaat tatccgaaca
    15241 aataagatgg attaaagctt ctctacaaat agattatgca gatttattta aacagcatgg
    15301 ttcgttaaat ggacagattc agcaacgttt atcagattgg caatatcagg ccaaactggc
    15361 tttgttgcag aaaaacccat ttatattaca tgaagaaaag ctaaaaaaac accaacaaac
    15421 agaaattttt aatcaacagt ctgttgatga ttatcggtat ttttgcaaat tagcaggcgt
    15481 gccatttgaa aaagaattct ggcagcagga attaacacaa agcaaaaatt caaaacccaa
    15541 taaacagaat ttgcctttga aattgctaac aggaaaatgg cagcagcaat tagactatgc
    15601 caaatctcaa tggtatttaa agcaacttaa tcaattacga caggaattta tagataagtt
    15661 aaaacaggaa ctcgacacta tccgacagct tgcagaacag ttagagcaat ttggttttga
    15721 agcagggctg tggtttgata acagtatcgg caatttaagc cagcaaaata ttgaagaaat
    15781 gaaacgctgg ctgaattact tgacccaaga taagaatgcc caacagattg ctgaattact
    15841 aggcaaaatg cgccaaatcg aacaatctga aaaaatcgaa caggtaaaac agactgttta
    15901 cacaaaaagc ccacaaattg atgtgaattc gcgtgaagaa attatcggat tacgtttagg
    15961 taaagattta gagtatgtat tgccgtctga attggcttta atggcagatg aagaaacatc
    16021 gactttattt gatttgaagt ttttggaatc caaattaatg tgttttgaat tgcaaggaat
    16081 gacctattgc gatgccccaa ctgaaattat agttgaacaa aaaagccaag aggatgaaaa
    16141 gctcggacca atgattttat gtgtagatac cagtggttca atggttggtc tacctgaaaa
    16201 tattgccaaa gcaatggctt tatttttagg agcaaaagct aaaagcgaaa accgttcttg
    16261 ttttgttatc aatttttcca ctggaataga aacttttgaa ttaacaagta aaacagggat
    16321 ttccaatttg attgcttttt tacgtcaatc ctttcatggt ggtacagatg ctgccccagc
    16381 tttacgtcat gcgctaaaaa tgatggagca agaaatctat caaaaggcag acttattaat
    16441 gatttccgat tttgtgatga acagcttacc tgatgatttg ttggcatcaa tcgaaataca
    16501 acgagaaacc ggtaatcagt ttaattcttt ggttattggt gatgctttta tgagtaaacg
    16561 cctaaaaaca cattttgatc gtgagtggat atacaatcct aatgttcaga caattcaaga
    16621 attagtacag ttcaagaaag atattttcaa taaacaagtt gtcaactcat aacactatcc
    16681 tactcatatg tatttttgga agataccacc ctatacaact tggaatccat acgcagaata
    16741 tgtgtggact ttctaaacta ctcccctttc caccctaaaa aaggtcgtct gaaacccatt
    16801 ttcagacgac ctttttccac ttcgcaaaac aagaattccc acccctctcc ccaaacgtcg
    16861 cccgaaccag taaactcccc atatttcaac aacttcttgg aagccaacca tgccccgtat
    16921 ccaccctcct cacctgttcc cgccccatat cgccgaacgc ggccttttgt attttcagca
    16981 gggcaaggtt cgcgatgtcc gaaaaacttc cgccgggcgt tatcgggcct ggcgccgttt
    17041 gctgccggcg gtctgaataa tggtggcgtc aacgacggca gcggatgctt tctctacttt
    17101 taggtttttt tcggtcagtt ggcggttaat cagtttgagc aattcggaca gggtgtcgtc
    17161 ttgcgccagc cagttgcggt aacggcataa ggtgctgtaa tcggggatgc tcagttcgtc
    17221 aaagcggcaa aacaggttga agtcgatgcg ggtgatgagg ctgtgctcga gttcgggatc
    17281 ggagaggctg tgccattgtc cgagcagaac ggctttgaac atggacaaca gcgggtaggc
    17341 gggacgaccg cggtggtctc ggaggtaacg ggttttttga cggttcaggt attgttcgat
    17401 cggttgccaa tcaatcacct ggtccaactt caatagtggg aaacggtcga tgtgtttggc
    17461 aatcatggct tgggcggttt gctgaaagaa ggtgctcatg agaaatctcc taaatgtctt
    17521 ggtgggaatt taggggattt tggggagttt tgcaaaggtc tcagtctttt taaaccacag
    17581 cccttcccaa ctaaaccaaa aggtcgtctg aaccctattt tcagacgacc ttttgccact
    17641 ttgtaaaaca aatcttccca ccatcctctc cccaaacatc gcccgaacca gtaaacttct
    17701 catatttcaa caacttcttg gaagcaaacc atgccccata tccacccgct ccacctgttc
    17761 ccgccccata tcgccgaacg cggactgctc tattttcagc agggcaaagt tggtgctgtc
    17821 caaaaaactt ccaccgggcg ttatcgggcg gaggtatgcg gtttggaaaa ctattgggta
    17881 tggctgaagc tggatagtga tttgtatatt aaagacgaaa gctgcgattg cccttatgtc
    17941 taaaggtgca aacatacctt aaattactat attacatagg caaaatacaa gcctataacg
    18001 aattggaaac aaaatgccgt ctgaaagcat tataaaatct gttcaccttt tcagatgagt
    18061 aatgtacacc cctatacaat ttttgctact ttaccccata aatccacggc taaagatatc
    18121 cttattatgt cctatgattt atcgaaacga cttgtaatcg gcttagcatc aagtgcccta
    18181 ttcgacttat ccgaatcgga taatatattt agaatggaag gggcagaaac ctataggcaa
    18241 tatcagagag aaaaacaaaa ccatccccta aaaaaggcgt tgtctttcca tttattaaaa
    18301 aacttctgtc aatcaatgaa ataaacccaa acgacccaac gattgggttt attcttttat
    18361 ccagaaacaa tccagataca gattacaagt cataactata ggcttaatat tacacgattc
    18421 tcattccatc aaggcggaaa accgcacaaa tactgaaaca ctatcgatcg atttgtaaac
    18481 aagcctactt aagtaacttg cagtccttat catttccttt aaaataatcc agcccgtcac
    18541 tacacgaact ggcggacttc ttgcaaataa aggttactag attttcattc atcttaataa
    18601 taaaaggatt tttatcttta tctatggcta ccgccttcaa catgaattta ctgtctaagg
    18661 ccccgcgcgc gattccattc aaacggatac aaaagccttc tgcctcttta atcggcaaac
    18721 ttggccattt ggtagatgtt tgtttaaatc tcccattctg cagataaaac ttttccataa
    18781 aatgtgcatt ttctaacaag gctgcccgca ctgcatttat ctttgctttc tcaacataat
    18841 tgcgatagct cggataaaca attaaagcaa gtacagacaa tatcaagacc actgatatta
    18901 attcaaccag cgtaaacccc cgattatcag tcattacttt acttccaata agaacagatt
    18961 attcaacata tttctttgaa cagacttact atcccattca gcagtatgca tatttcccac
//
LOCUS       DAXHTR010000022        18343 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.022, whole genome shotgun sequence.
ACCESSION   DAXHTR010000022 DAXHTR010000000
VERSION     DAXHTR010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18343
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.022"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            321..584
                     /locus_tag="ACFNML_000548"
     CDS             321..584
                     /locus_tag="ACFNML_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391971.1"
                     /translation="MPSAQQANCPQKLFFMKNNPQVCTKGNPPYRHPFKTEIPMIFKI
                     KKSYPQKISPINIIIVFLFKFYILFISLQGNGTGKRKGIFSKN"
     gene            664..2112
                     /gene="gnd"
                     /locus_tag="ACFNML_000549"
     CDS             664..2112
                     /gene="gnd"
                     /locus_tag="ACFNML_000549"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HFC6391972.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCRFKVVAYNRTIGKVDEFLNG
                     AAKGTGIIGAYSLQDLVDKLEKPRKIMMMVRAGSVVDDFIEQLLPLLEKGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGMGVSGGEEGARHGPSIMPGGDERAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKNGLGLSYDEMHRV
                     FTAWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARTVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLSNIRDAYENNPDLVFLGADPY
                     FKNILENCLPAWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2175..3446
                     /gene="waaA"
                     /locus_tag="ACFNML_000550"
     CDS             2175..3446
                     /gene="waaA"
                     /locus_tag="ACFNML_000550"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFC6391973.1"
                     /translation="MFQWLYDVLWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGVLMETEIWPNLMKECRRAGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFEKRIGGRPVAVCGSTRVYRGEDEAEKLLAAWQQYRGDALLVVVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVRVESADAWREAVEKTLSSEGGGMQMQ
                     AHVDGFIAQHRGAGARIAEAVREAVCGHRGR"
     gene            3487..3936
                     /locus_tag="ACFNML_000551"
     CDS             3487..3936
                     /locus_tag="ACFNML_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HFC6391974.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGVVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            3969..4943
                     /locus_tag="ACFNML_000552"
     CDS             3969..4943
                     /locus_tag="ACFNML_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFC6391975.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLSFIGVKMLVMHWVHIPISVSLSVVFGALGASILTSLIYTKKQPDK"
     gene            5012..5087
                     /locus_tag="ACFNML_000553"
     tRNA            5012..5087
                     /locus_tag="ACFNML_000553"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5045..5047,aa:Lys,seq:ttt)
     gene            5307..6560
                     /gene="murA"
                     /locus_tag="ACFNML_000554"
     CDS             5307..6560
                     /gene="murA"
                     /locus_tag="ACFNML_000554"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFC6391976.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGTLRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVKAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGA
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
     gene            6641..7819
                     /locus_tag="ACFNML_000555"
     CDS             6641..7819
                     /locus_tag="ACFNML_000555"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFC6391977.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASI
                     QYCMDNGASVIVMIHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDDMILDIGPKSAAELAELLKAAGTVVWNGPVGVFEFDQFAGGTKALAEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGA
                     "
     gene            complement(7886..8134)
                     /locus_tag="ACFNML_000556"
     CDS             complement(7886..8134)
                     /locus_tag="ACFNML_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990229 - iron-sulfur cluster assembly
                     complex [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HFC6391978.1"
                     /translation="MLTPEQVKAMIEDVAKCEHIKVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWAAKAQ"
     gene            complement(8224..9141)
                     /gene="ftsX"
                     /locus_tag="ACFNML_000557"
     CDS             complement(8224..9141)
                     /gene="ftsX"
                     /locus_tag="ACFNML_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFC6391979.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITIYMETAAAQSDSDTVRSLLARDKRLDNIRFIGKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESASMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPEEIV
                     LVFGFVIALGVFGAWLATTQHLLGFKAKK"
     gene            complement(9138..9788)
                     /gene="ftsE"
                     /locus_tag="ACFNML_000558"
     CDS             complement(9138..9788)
                     /gene="ftsE"
                     /locus_tag="ACFNML_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFC6391980.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPGLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(9785..9976)
                     /locus_tag="ACFNML_000559"
     CDS             complement(9785..9976)
                     /locus_tag="ACFNML_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391981.1"
                     /translation="MPSESASDGIFPARPSVSAKLIHYLKTGGIFIIGTVLPIVQTAY
                     PCGRNRPKRYAAHSLQDLL"
     gene            complement(9984..10466)
                     /locus_tag="ACFNML_000560"
     CDS             complement(9984..10466)
                     /locus_tag="ACFNML_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HFC6391982.1"
                     /translation="MKYLNLAAITLGATFAAHTASADELAGWKDGAAQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTGANSRNFMKTYGNTVGVLPFTVVEAPKCGYRQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            10590..10943
                     /gene="arsC"
                     /locus_tag="ACFNML_000561"
     CDS             10590..10943
                     /gene="arsC"
                     /locus_tag="ACFNML_000561"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFC6391983.1"
                     /translation="MSEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLESARGMMRVKDDLYKELGLDNPDLDNNALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            10940..11596
                     /locus_tag="ACFNML_000562"
     CDS             10940..11596
                     /locus_tag="ACFNML_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HFC6391984.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFTSSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            11712..13106
                     /gene="gltX"
                     /locus_tag="ACFNML_000563"
     CDS             11712..13106
                     /gene="gltX"
                     /locus_tag="ACFNML_000563"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFC6391985.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLDYDNAGNVVYQTRRFDRYKEVIAELLEKGDAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTKWTDL
                     VKGEISIPNEALDDLIIARADGTPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDLFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            13127..13522
                     /locus_tag="ACFNML_000564"
     CDS             13127..13522
                     /locus_tag="ACFNML_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6391986.1"
                     /translation="MPSDVGMRLQTAFKRKLNMKKILYFLMFVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQTAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDYAWNAVYGAGGTKASGN"
     gene            13526..14017
                     /locus_tag="ACFNML_000565"
     CDS             13526..14017
                     /locus_tag="ACFNML_000565"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6391987.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIH
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMESFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            complement(14140..16734)
                     /gene="mutS"
                     /locus_tag="ACFNML_000566"
     CDS             complement(14140..16734)
                     /gene="mutS"
                     /locus_tag="ACFNML_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFC6391988.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQMDGVPIKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNAPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCTTHMGSRLLALWLHHPLRNRAHIRARQEAVTALDSQYEPL
                     QCRLKNIADIERIAARIAVGNARPRDLAALRDSLFALSEIDLSTNGSSLLETLKAVFP
                     ENLSTAEQLRQAILPEPSVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPEF
                     ADYPVIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHL
                     SALEQGQDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNGLENQAAANRP
                     QLDIFSTMPSEKGDEPNVGNFVDKAEEKHFEGILAAALEKLDPDSLTPREALSELYRL
                     KDLCKSVS"
     gene            17178..18182
                     /gene="gap"
                     /locus_tag="ACFNML_000567"
     CDS             17178..18182
                     /gene="gap"
                     /locus_tag="ACFNML_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6391989.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHGIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPSGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTSDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
BASE COUNT         4203 a         4468 c         5284 g         4388 t
ORIGIN      
        1 actttcgtgg gaatgacggg atgtaagttc gtgggaatga cgcggtacag gtttccgtta
       61 cgtatcccca tccgaaagtt tacttataca caagcaatcc aaagttatct gactgaaata
      121 ttgtgagatt tacccgcaaa tctcattttc tccaccactt atacacaaac tcggatgcac
      181 actctgccaa tatgcagaga aaaagacatt cgtgccgtta acttactatt cccacacgtt
      241 ccaaacacat ttccctctca aacctccaca gtattcccgt tcttaaaggc gccagcagcc
      301 attcggtaaa gaaaagtagg atgccatctg cacaacaagc aaactgtcca caaaaactat
      361 tcttcatgaa aaacaatcca caggtatgca caaaagggaa tccgccttac cgacaccctt
      421 ttaaaacgga aatcccaatg atttttaaaa taaaaaaaag ttacccacag aaaatttctc
      481 ctatcaatat aatcatcgtt tttttattta aattttatat tttatttata agccttcagg
      541 gaaacggcac gggaaagcgt aaaggtatct tttcaaaaaa ttaaaaagtt aaggattgtt
      601 aaacaagtgt aaatcaggta gagttgcggt cgataaatta aatctataac ggagcttcct
      661 gcaatgaaag gcgatattgg cgtaatcggt ttggcggtta tggggcaaaa cctgattttg
      721 aatatgaacg attgcagatt taaggttgtc gcctacaacc gaacaatcgg taaggtggac
      781 gaatttttaa acggcgcggc aaaagggacg ggcattatcg gcgcgtattc cctgcaagac
      841 ttggtcgata agctggaaaa accgagaaaa atcatgatga tggttcgcgc aggttcggtg
      901 gttgacgact ttatcgaaca actgcttccg ttgttggaaa aaggcgacat cattatcgac
      961 ggtggcaatg ccaattaccc cgacacgaca cggcggacac attaccttgc cgaaaaaggg
     1021 attttgtttg tcggcatggg ggtctccggc ggagaggaag gcgcgcggca cggcccgtct
     1081 atcatgccgg gcggagatga gcgcgcatgg gaagctgtta aaccgatttt ccaggcgatt
     1141 gctgccaaaa cgccgcaggg cgaaccgtgt tgtgactggg tcggcaagga cggcgcggga
     1201 cattttgtca aaatggtgca taacggtatc gaatacggcg atatgcagtt gatttgcgaa
     1261 gcgtaccagt ttatgaaaaa cggtttggga ctgtcctacg acgaaatgca ccgcgtgttt
     1321 accgcgtgga acaaaaccga gctggattct tatctgattg aaatcacagc ggcaattttg
     1381 ggctataagg acgaaggcgg cgaaccgctg gtcgagaaaa tcctcgatac ggcagggcaa
     1441 aaaggcacgg gcaaatggac gggcatcaat gctttggatt tgggtattcc gttgacgctg
     1501 atttccgaag cggtattcgc ccgctgcgtt tcgtcgttca aagaacagcg cgtgcagacc
     1561 ggcaagctgt tcgcccgaac cgttacgccc gtcgaaggcg gcaaacaaga atgggttgag
     1621 gcgttgagac aggccctgct tgcatccaaa atcatttcct acgcacaagg ctttatgctg
     1681 atccgcgaag cgggcgaaag ctacggctgg gatttggact acggcaacac tgcgctgctg
     1741 tggcgcgagg ggtgcatcat tcgcagcgca tttttaagca atatccgcga tgcctatgaa
     1801 aacaatcccg atttggtgtt cttgggtgcg gatccgtatt tcaaaaatat tttggaaaac
     1861 tgcctgccgg cgtggcgcaa ggtggttgcc aaggcggtcg aatgcggcat ccctatgccc
     1921 tgcatggctt cggcgattac gttcttggac ggctacacga ccgaacgtct gcctgccaac
     1981 ctcttgcagg cacagcgaga ctacttcggc gcgcacacgt acgaacgcac cgacaaaccg
     2041 cgcggcgagt ttttccatac caactggacg ggcaagggcg gagataccgc ttcgacaacc
     2101 tacgatattt gattccgttg ccgaaatgcc gtccgaaccg ttcggacggc atttgttttg
     2161 caggaaatag aaagatgttc caatggcttt atgatgtatt gtggctgctt gcgccgatat
     2221 ggatacggcg ttatttggac aaacgctccg gaagtgcccc tgcatatcgg gcgcatcggg
     2281 acgagcgttt cggcaagccg tatccgaatc ccgttaccgg cgcggtttgg atacacgccg
     2341 tttcggtcgg ggaaacgcgt gccgcccaac ctttgatacg cgagttgcgg cagcgttttc
     2401 ccgatgcgcc gctgctgatg acgcagatga ccccgacggg acgggaaacc gcgcaagttc
     2461 tgtttcccga tgcgcaatgc cgctatcttc cgtatgacaa aaaaacgtgg gtacggcagt
     2521 ttttgcgcga acaccgcccg atgttcggcg tattgatgga aacggaaatc tggcccaacc
     2581 taatgaagga atgccggcgc gcgggtgtgc cgctgttttt ggcgaatgcg cggctgtcgg
     2641 aaaaatcgtt gaacggttat ctgaaagtcc gccgcctgat ccgtcctgcc gccgcttcgc
     2701 tgacgggttg tctggcgcag acggaggcgg atgcggcgcg gttggcgaaa ttgggcgcgg
     2761 catccgtgca ggtgtgcggc aataccaaat acgacctgat gccgtcggaa cagatgaaaa
     2821 cgctggcggg gcagtttgaa aaacgcatcg gaggccggcc ggttgccgtg tgcggcagca
     2881 cgcgcgttta tcggggcgaa gacgaggcgg aaaaactgct ggcggcgtgg caacaatatc
     2941 gcggcgatgc gctgctggtc gtcgtgccgc gccatcccga gcattttcag acggtatttg
     3001 aaacggcaaa acgcttcggg tttaaggttc agcggcgcag cgacggtttg ccggtcgaac
     3061 ctgatacgca ggtgtggata ggcgacagta tgggcgagct gtatgcgtat tacctgtgcg
     3121 ccgatgtcgc ttttgtcggc ggcagtctgg tcgattcggg ctgtcagaac attatcgaac
     3181 cgctttcctg cggcgttccg acgatattcg gcttttcaac ctacaatttt tccgaagcct
     3241 gccggcacgc cttggcatcg ggggcggcgg ttcgggttga gtcggcggac gcgtggcggg
     3301 aagccgttga aaaaacctta tcgtccgagg ggggcggaat gcagatgcag gcgcacgtgg
     3361 acggctttat cgcacaacat cgcggagcgg gcgcgagaat cgccgaggcg gtacgggaag
     3421 cggtatgcgg acatcgggga cgatagtgat acaatccgta tccgagtttt ccggttggag
     3481 catcgtatga gtatttatgc cgtcgcgcac atcgtccacc tgtattgcgc cattgccttt
     3541 gtcggcggcg tgttttttga agtgctggtt ttgtccgtcc tgcatacggg acgggtgtcg
     3601 tgcgaggcgc ggcgcgaagt ggaaaaggca atgtcttacc gcgccgtcag ggtgatgccg
     3661 tttgtggtcg gactgctgtt cgccagcggc gtcgtgatgg cggcaaaccg ctatctttct
     3721 atattgggcg aaccgtttgc cacttccttc ggtacgatgc tgacgctgaa aatcctgttg
     3781 gcgttcagcg tgttggcgca cttcgccatc gccgtcgtca aaatggcgcg ttccacactg
     3841 accgtcggct ggtcgaaata catacacacc gtcgtcttta cccatatgct gctgattgtc
     3901 tttttggcaa aagcgatgtt ttatatcagc tggtaacaca attacatttc aaatcaggag
     3961 attgaaccat gacagaatat cccggcatcg ggtcgccgtt gttttacggc gttttttttg
     4021 cggcagtgct ggtcatgatt gccttggata tgttttcgct gaagaaaaac ggcagccata
     4081 aagtcggcgt caaagaagcc ttggcatgga gcggcttgtg ggttgccgta tcctgcctgt
     4141 tcgcgggctg gctgtatttt gaactcgccg gcaatctcgg ctacggcgcg gcagtcgcaa
     4201 aagaaaaagt attggaattc tttaccggct acattttgga aaaatcgttg gcagtcgaca
     4261 atattttcgt gttcctgatg atattcggct acttcaaagt cgcaccgcag tttcagcacc
     4321 gcgtgctgct gtacggcgta ttgggcgcat tggtattgcg cactgtcatg attttcgtcg
     4381 gcgcggcact ggttcagcag tttgagtgga ttctgtatct gtttggcgcg ttcctgctct
     4441 ataccggcat acacatgatg aagcccgaag gcgatgagaa agaggatttg gcaaacagca
     4501 ggctgctgaa tgccgtcaag aaagtcgttc cggttggcac ggaatttcac ggcgagaaat
     4561 tttttaccgt cgaaaacggt aaaaaaatcg ccacgccgct gtttttggtg ctggtcatga
     4621 ttgaattgag cgatgtcgtg tttgccgtgg acagcatccc cgccgtcttt gccgttacca
     4681 ccgatccgtt tatcgtgctg acttccaata ttttcgctat tttgggcttg cgggcgatgt
     4741 atttcctgct ggcggatgtg gcggaacgct ttatcttcct gaaatacggc ttggcattcg
     4801 tgttgagttt tatcggtgtg aaaatgctgg taatgcattg ggtgcatatc ccgatttccg
     4861 tttcgctgtc ggtcgtgttc ggcgcgttgg gtgcatcgat actgacctcg ttaatttata
     4921 cgaaaaaaca gcctgataaa taaaatgccg tctgaacgcc gacaaaatgg ttgacagcat
     4981 caggcttgat gtatagaatt tcgagcttat cgggtcgtta gctcagtcgg tagagcagcg
     5041 gacttttaat ccgttggtcg agcgttcgaa tcgctcacga cccaccagat aaaacaaaaa
     5101 aagctgagtt ttcaaaactc ggcttttttt gtcttccgac aatccttgtt cggaaagcgc
     5161 ggcatttcgt caaattcttt ggtgcggctt ccgtattttt cccgaaaatc ccttataatt
     5221 ccgcctctgc aaccttcatc gggtgatgct ttatgcgttg tcccgagtat tgaaatatta
     5281 caagtaccca ccaggaaaga acaaacgtgg acaaactgaa aatctccgca aacggcccgc
     5341 tcaacgggga aataacggtc tcgggcgcga aaaacgcggc attgccgctg atgtgcgcgg
     5401 gtttgctgac atcgggtacg ttgcgcctga aaaatgtgcc tatgctggca gatgtgaaaa
     5461 ccacacaaaa gctgcttcag gggatgggcg cgcgcgtcct gaccgacaat atcagtgaat
     5521 ttgaaatcaa cggtggtacg gtaaacaaca cctgcgcccc ttacgaattg gttcggacga
     5581 tgcgggcctc gattttggtg ctggggccga cgctggcgcg tttcggcgag gcgcaagtca
     5641 gcctgccggg cggctgcgcc atcggttcgc gccccgtcga tcagcatttg aaaggcttgg
     5701 aagcgatggg tgctgagatt gttatcgaac acggttacgt caaagccaaa ggcaaactca
     5761 aaggtacgcg cgtggcgatg gatgtcgtta ccgtcggcgg cacggaaaac ctgctgatgg
     5821 cggcgacgct ggcggaaggt acgacggttt tggaaaactg cgccattgag cctgaagtgg
     5881 tcgatttggc ggaatgcctg gtcaaaatgg gcgcgaaaat cagcggcatc ggtacgtcca
     5941 caatgattgt ggaaggtgcg gacgagctgc acggctgcga acacagcgtc gtccccgacc
     6001 ggattgaggc ggggacgttc ctgtgcgcgg tggcgataac cggcggcagg gtggttttgc
     6061 ggaatgccgc gccgaaaacg atggaagtgg tgttggacaa actggttgag gcaggtgcgg
     6121 tgattgaagc gggcgacgat tggatcgcca tcgatatgcg gcagcgtccg aaggcggtgg
     6181 acatccgcac ggtcgtccac cccggcttcc ccacggatat gcaggcgcag tttatggcat
     6241 tgaatgccgt ggcggaggga agctgccgcg tggtggaaac gatttttgaa aaccgcttta
     6301 tgcacgtccc cgagttgaat cgaatggggg cgaacattac aaccgagggc aatacggcat
     6361 ttgtgcaggg tgtggaacag ctttccggcg cggtcgttaa ggcgacggat ttgcgtgcgt
     6421 ccgccagcct cgttatcgcc ggtttggcgg cgcgaggcga aaccgtggtc gaacagattt
     6481 accacttgga tcgcggttat gaaaatattg aaaaaaaact cggcagcgtc ggcgcgaaaa
     6541 tcgagcgcgt atccggctga tcgggttgcg atgccgtctg aagccttcag acggcatcaa
     6601 ttgtttctgt atcgcaaaca ctgattgaaa aggactgatt atggcatttt taaaactgac
     6661 cgaacaaaac gtgcagggca aaaccgtcct catccgcgcc gatatgaacg tgccgttcaa
     6721 agacggcaaa atcagcgacg acacccgtat ccgcgcctcg ctcgcgtcca tccaatactg
     6781 catggacaac ggcgcatccg ttatcgtgat gatccacttg ggtcgcccga ctgaaggcga
     6841 gttccacccc gaagacgacg ttgcgcccgt tgccgcgcat ttgggcggtc tgttgggcaa
     6901 agacgtgaaa gtattgaacg actggcgcga aaacaaaccg gctttgaacg cgggcgatgt
     6961 cgtgatgctg caaaacgtgc gtatcaacaa aggcgagaag aaaaacgatt tggaactggg
     7021 taaagcctac gcttctttgt gcgacgtgtt cgtcaatgac gcgttcggca ccgcgcaccg
     7081 cgcccaagct tcgaccgaag ccgtcgctca agccgcgccc gttgcctgcg ccggcgtatt
     7141 gatggcgggc gaactcgacg ctttgggcaa agccctgaaa cagcccgcgc gcccgatggt
     7201 tgccatcgtt gccggcagca aagtgtccac caagctgacc atcctcgaat cgctggcgga
     7261 taaagttgac caactcatcg tcggcggcgg tatcgccaac accttcctgt tggcggaagg
     7321 taaagccatc ggcaaatctt tggcggaaca cgatttggtg gaagaatcca aaaaaattat
     7381 ggcgaaaatg gcggccaaag gcggttctgt gccgctgccg accgatgtcg tcgttgccaa
     7441 agcctttgcc gccgatgccg aagcggtcgt gaaagacatt gccgacgttg ccgaagacga
     7501 tatgattttg gacatcggtc cgaaatccgc cgccgagctt gccgaactgc tcaaagccgc
     7561 aggcacggtc gtttggaacg gccctgtcgg cgtgtttgag tttgaccaat tcgcaggcgg
     7621 cacgaaagct cttgccgaag ccattgccca aagcaaagca ttctcgattg caggcggtgg
     7681 cgacacgctg gcggcgattg ctaaattcgg cgttaccgac caaatcggct atatttctac
     7741 cggcggaggc gcgtttcttg agttcttgga aggtaaagag ttgcctgccg tagccgcttt
     7801 ggaaaaacgc ggcgcgtaag cggtttgata taaaaaatgc cgtctgaaac gttgtgctgt
     7861 ttctgacggc atttttgtgt ggcgattatt gtgctttggc tgcccattcc gccggagtgg
     7921 cggcaaccga aatggaaagt gcgtgcagtt cgttactttc cagttgggct ttgagtccgt
     7981 ctttaatcag gcggtggcgc gcgaggcgtg ccttgccttc aaattctgat gaaacgatga
     8041 cggcgaaaaa atggtgtccg tcgccttcta ctttgatatg ttcgcatttt gccacgtctt
     8101 caatcatggc ttttacttgt tccggggtca gcatggtagg ttccttgttg gaaaaatgcg
     8161 ccattatact ggcaaattga aatgccgtct gaaacgggtt cagacggcat ttttgacggt
     8221 gttttatttt ttggctttga agccgagcag gtgctgggtg gtggcaagcc acgcgccgaa
     8281 tacgcccaac gcgatgacga agccgaacac cagcacgatt tcttcagggt aaaagaaacg
     8341 ccagccgatg ttcaagccgt agggtttgaa aatggcatcg accaatgggc gcacggcaga
     8401 gagcagccaa ccgcaaagcc ccaagctgac ggcggcggaa aggatgctct gccacattgc
     8461 ctgataaagg aaggggcggc ggataaacga cgcgggcgcg cccaagagtt tggtgatttc
     8521 gatttcttct tttcggctga ggatttgtag gcggatggtg ttgtgcgcga caaggacgaa
     8581 cgccatcccc agcgtcaggg aaagaaacca taaaattttg cggatgaact cgttgatttg
     8641 gtacagcgtt tgcacccatt cggtatccat agacgcggat tcgaccatag gcagtttggt
     8701 aatgtctcgg tagattgcct gcatttgggc gggcgtggtt gccgggtcgg gggtaacgat
     8761 aaagacatcc ggcagggggt tgccgtcaag catggaaatc agattttggt cgagattgga
     8821 ctgtaattct tccagaccgt cttctttgcc gatgaagcgg atgttgtcga gccgtttgtc
     8881 gcgcgccagc aggctgcgca cggtatcgct gtcgctttgt gcggcggcgg tttccatata
     8941 gattgtgatt tgcggcgact cgttgagttt gcccaacacg ctttgcccgc tttggatgcc
     9001 caaatacata aacagcggca gggtcatggc gacggcgagc attatgaggg taagcagtgt
     9061 gccgaaaggt tggcgcagaa gctgtttgag cgcggtgcgc gcggattcga cgtgcagcga
     9121 gaggtagtgg atgatgctca tgcgagtcgt cctttcgaga ggcgcaggat gcggtgtccg
     9181 tagtccgcca tcagggtttc gtcatgtgcg gcgacgatga cggttgttcc cgcttcgtgg
     9241 aaggttttga acaattccat aatatcgagc gcgtaggcgc ggtcgaggtt ggcggagggt
     9301 tcgtcggcaa tcagcaggcc gggctggtga acgacggcgc gggcgatgca caggcgttgt
     9361 tgttcaccgc cggagagggt tacgggatcg tccaattctc gtcctttcag gccgactttt
     9421 tcgatggcga tgcgggcacg ctcttcggct ttgcgcggcg gatagccgat aatccgaagc
     9481 ggcaggatga cgttttgcag gacgttgcgg tcgtagagga ttttgtggtc ttggaacacg
     9541 atgccgatgt gttggcgcat aaagccgatt tggttgtcgg acaatgtgcc gaggtcctgc
     9601 ccgttaaaca ggattttgcc cctgcttggt ttggtaatgc ccgaaatcag cttgaggacg
     9661 gtggatttgc ccgaaccgga gtgtcccgcg ataaatatca tttcgccttt gttgatttgg
     9721 aagctgacgt ttttcagggc ttcaaaaccg ccgggatagg ttttggaaac ttgttcgaaa
     9781 cggatcatag gaggtcctgt aaggaatggg cggcatatcg tttcgggcgg ttgcgtccgc
     9841 agggatatgc cgtctgaaca ataggtaaga cagtgccgat tataaagatt ccgcctgttt
     9901 ttagatagtg gataagtttg gcggataccg aaggtcgggc gggaaaaatg ccgtctgaag
     9961 cggattcaga cggcatccgg cgtttaacgg cattttgaat gggcgagttt gacggcttcg
    10021 gtcaggcttt tttcgttcac ttccccggta atggtctgcc tgtatccgca tttcggtgct
    10081 tcgacgacgg taaagggcag tacgccgaca gtgtttccgt aggttttcat aaagtttcgg
    10141 ctgttcgccc cagtgtaacg ccaaatcggg taagaaacag gggtttgctt gaggaagttg
    10201 ccgatattgt cggatgtgtc gagcgcgatg ccgaccatat cgacgctgcc ttttttctgc
    10261 gctttgtacc atttggacat ggcaggcatc tcttttcggc acggaccgca ccaggtcgcc
    10321 caaaggttga cgatgcggac gggggctttg agcgattgca ggctttgcgc ggcaccgtct
    10381 ttccatccgg ccagttcgtc tgccgaggcg gtatgtgcgg caaatgtggc gccaagggtg
    10441 attgcggcaa gattcaagta tttcatcgtt ttgtcctttg tatgtcgatt cgggtattgt
    10501 aaaccgtttg gtacggttgt aggcgggata ttgagtaaaa ccctgtgtaa gccgataata
    10561 aggggattgt cgagaagtaa ggaaatgtca tgtctgaaat caaaattttc cacaatccgc
    10621 gttgcagcaa atcgcgtgcc gccttgtccc tattggaaga acgcggcatt gctgccgaag
    10681 tggtcaaata tttggatacg ccgcccgact tgtccgaatt gaaggatatt ttcaacaaat
    10741 tgggcttgga gtcggcgcgc gggatgatgc gtgtgaaaga tgatttgtac aaggaattgg
    10801 gtttggacaa ccccgatttg gacaataacg cactgctgcg tgccatcgcc gatcatcccg
    10861 ccctgttgga gcgtccgatt gttttggcaa acggtaaggc ggctgtcggc aggccgttgg
    10921 aaaatatcga agcggtattg tgaacaaacg gctgttttgc agccgcaacg gcttgcgcta
    10981 ttacctgttg ggcggtttct gtttgagtgt ttttccgctt ttgctggtgt ttacctcatc
    11041 cgtttgggcg gtttatcgga cgggcgggca ggttttgccg ccgtatgtcc gggcggatgc
    11101 cgcgctggtg ttgggtgccg ccgcatggga caaacgccct tcccctgttt ttcgggaacg
    11161 catcaaccac gccatcgcgc tttaccaaag ccgcagggta ggcaaaatcg tgtttacggg
    11221 agggcgaacc aaaaaaggct atatgaccga agccgaggtc gggcggcggt acgcgctcaa
    11281 acagggggtt ccggcgcgca acatcctgtt tgaaaacact tcgcgcaaca cttatgaaaa
    11341 cctgaacaac atccgtccgg ttttgcgtgc caacggtatt gccagcgtgg tgattgtcag
    11401 cgatccctac catttggcgc gggcggcgga aatggcggag gatttgggtg tccgcgttta
    11461 tatgtctgcc accccgacca cgcggtttga tgcgggaaat aagaaaaaaa tctttatgct
    11521 gcaagaggga tatgcgttat ccctctaccg tttggaaaaa tggggcagcc gctttttggg
    11581 atggctgtct gattgaagcg gtagaaaatg ccgtctgaaa cttatccgca ggcagggttc
    11641 ggacggcatt tttaatgccc aagcacggta aaaacagtaa aataagccgt tttaaagatt
    11701 ttggagccat catgaccgtc aaaacccgtt tcgcccccag cccaaccggc tacctgcaca
    11761 tcggcggcgt acgcaccgcc ctgttttctt gggcgtttgc ccgccatcat aaaggcgaat
    11821 tcttattgcg catcgaagac accgacttgg cgcgctctac tgccgaatcc gtcaacatca
    11881 tcctcgacgg catgaaatgg gtcggactcg attacgacaa cgccggcaac gtcgtgtacc
    11941 aaacccgccg tttcgaccgc tataaagaag tcatcgccga acttttagaa aaaggcgatg
    12001 cctactactg ctattgcagc aaagaagagt tggaagccat gcgcgagaaa gccgaaaaag
    12061 aaggcacggc gacttacgac cgccgctggc ggccggaagc aggcaaaacc ctgcccgaaa
    12121 tccctgccgg cgtgcaaccc gtcgtccgct tcaaaacgcc tttggatggc gtaaccaagt
    12181 ggacggactt ggtcaaaggc gaaatctcca tccccaacga agcactcgac gacctgatta
    12241 tcgcccgcgc cgacggcacg ccgacctaca acttctgcgt tgtcgtggac gactacgaca
    12301 tgggcgttac ccacgttatc cggggcgacg accatgtgaa caacaccccg aaacaaatca
    12361 acatcttaaa agccatcggc gctaccctgc ctgaatacgg ccacctgccg atgattctta
    12421 acgaacaagg caaaaaaatc tccaaacgca gcggcgatac cgttgccatt accgatttcg
    12481 gcgcgatggg catcctgccc gaagccatgc tcaactatct ggcacgtttg ggctgggcgc
    12541 acggcgacga tgagttcttc acgatggaac aatttatcga atggtttgat ttgaaagacg
    12601 tttctccatc tccaagccgc atggatttga agaaactcta ctggatcaac ggcgaacaca
    12661 tcaaaatcac gcccaacggc aaactcgccg aactcgtcaa accgcgtctc gctctgcgcg
    12721 atatccatga aactgaaaaa cctgcattgg aagacgtgtt ggcactggtc aaagaccgcg
    12781 cccaagacct gaacacactt gccgacgagt gcctctactt ctacgtcaaa caaacgccta
    12841 ctgaagcgga cgtgcagaaa cactgggacg atgaagccgc cgcccgtatg ctgcgctttg
    12901 ccgaacgcct cgaagggctg gaagactgga atgccgaagc catccacgac ctcttcaaac
    12961 ccttctgcga cgaagaaggc attaaaatgg gcaaactcgg catgcccctg cgccttgccg
    13021 tctgcggtac ggcgaaaacc cccagcgtcg atgctgtgtt ggcattaatc ggcaaagaag
    13081 aagtgttgaa acgcatccgc gcttaagtta tccacaggcg tgggcgatgc cgtctgatgt
    13141 cggaatgcgg cttcagacag cattcaaacg gaagttgaac atgaaaaaaa tattgtattt
    13201 tctgatgttt gttttttcta caagcgtatg ggccggggat gcggaagaca atctgctcag
    13261 catccaatcc ggctatcgtg ccttactgca aaaacaaaac aatctggacg gaaaaatcat
    13321 cgggatgcag tcggatttgg aagatgcgcg ccggcgtttg cagacggctc aagcagacat
    13381 cgcccgtttg gaagcggaaa ttcctgcagc aatggcgcaa aaagcccggc aggctgaaga
    13441 gctgaggcaa atcggagtgc gtttggacta tgcttggaat gcggtttacg gcgcaggggg
    13501 aacgaaagcg tcggggaatt gaccgatgaa ttccctcttt gtggacaata ctgttttcat
    13561 tacacggctg aaagccgggc atatcggcag gttggttcag gcgttgtttg aggagtggca
    13621 cggatttgaa ccgtggtctt ctgtggataa gattcatgcc tattacggca ggtgtttgaa
    13681 ggatgacgaa ctgccgctgg catttgcggc tgtggatgat ttcggaaccc tgttgggttc
    13741 ggctgcggtc aagcggcatg atatggaaag ttttccacag tatgaatatt ggttgggtga
    13801 tgtctttgtt ttacctgaat atcgcggaaa aggcattggc aggaggctgg tcgcccactg
    13861 cataggcgca gcgcgttcgc tggggataaa gttcttgtat ctttatacgc ctgatgtgca
    13921 aatattttat gaatcattcg gctgggcggt tgtcgggcga catttccata acggtgaatg
    13981 ggttacggtt atgcgtttgg atatggataa ggtttaagcc gaatggacaa atgccgtctg
    14041 agctgttcgg acggcatttt tatgtttgga aacttcttgt cagataatgt ttatccacag
    14101 atttttccat atggtttgat gctgttccga caacggaaat taagatacgg atttgcacaa
    14161 atctttcaga cggtacagtt ctgacaatgc ttcgcgcggg gtcaggctgt cgggatcgag
    14221 tttttccaag gctgctgcca atataccttc aaaatgtttt tcctctgctt tatccacaaa
    14281 gttgcccaca ttcggttcat ctcctttttc agacggcatg gtactgaaaa tatccagttg
    14341 gggacggttc gcggcggctt ggttttccag tccgttcaaa tgcttttggg cggatttcaa
    14401 tgcgcgtaca ggcaggccgg cgagtttggc aacggcgatg ccgtagcttt ttccggcggg
    14461 accgggttgg atttggtgca gaaacacgat gtcctgtccc tgttcgagcg cggaaaggtg
    14521 catattgagt gcggcggcgt gggcttcggg caggtaggtc agctcgaaat agtgggtggc
    14581 aaacaggctg aaggatttgt ttttttgcag caggtgttcg gcaacggcgt gcgcgagggc
    14641 gaggccgtcg aaagtggaag tgccacgtcc gacttcgtcc attaaaacaa ggctttgttc
    14701 ggtggcgtga tgcaggatgt aggcagtttc gctcatttcg accatgaagg tggagcggtt
    14761 ggaggcgagg tcgtccgatg cgccgatgcg ggtgaagatt tgatcgatgg gcccgattgt
    14821 ggcggcatcg gcaggcacga aacagccggt gtgtgccaat aaaacaatca gcgcgacttg
    14881 gcgcatgtag gtggatttgc cgcccatatt ggggccggtg agcagcatga ggcggtgttt
    14941 gtggtcaagg tcggtgtggt tggcggtgaa gtggcgtacc tgctgttcga caacgggatg
    15001 gcggccgttt tcgatgtgga taaccggata gtcggcaaac tcggggcgga cgaagttccg
    15061 ctcttttgcc aaggctgaaa atgtggacaa tacgtccagc gcggcggcgg ctttggcggc
    15121 tttttgaagc tgcggcaatg ccgtctgaag gtttttcaat acgccgtcaa agagttgttt
    15181 ttctaaggcg agggcttgct cttgagcagt cagtactttg tcttcaaagg ctttcagttc
    15241 cggcgtgatg aagcgttcgg cgtttttgag ggtttgccgg cgttggtagt cggcaggtgc
    15301 ttgttcggct tgggttttgg acaattcaat gtaaaagccg tgaacgcggt tgaactcgac
    15361 tttgagtgtg gacaaaccgg tacgttcgcg ttccttggct tccaaatcca gcaaaaattc
    15421 gtcgccatgg ttttgaatgc ggcgcaattc gtccagttcg ggatgaaaac cgtggttgat
    15481 gacattgccg tcttttagcc agacggaagg ttcgggcaaa atggcttggc ggagctgttc
    15541 ggctgtggat aggttttccg ggaaaacggc tttgagggtt tccaacaggc tgctgccgtt
    15601 tgtggacaag tcgatttctg acagggcaaa caggctgtcg cgcagggcgg cgaggtcgcg
    15661 cgggcgggcg ttgccgacgg cgatgcgggc agcaatgcgt tcaatgtccg caatgttttt
    15721 cagacggcat tggaggggtt cgtattggct gtccagcgcg gttacagctt cttggcgggc
    15781 gcggatgtgg gcgcggttgc gcaaagggtg gtgcagccag agagccaaga ggcggctgcc
    15841 catgtgggtg gtgcaaaggt cgagcgtgga catcagggtc ggcgattttt tgccggagag
    15901 ggtttgcgtg atttcgagat tgcggcgcgt ggcggcatcc ataccgatat attggctgtc
    15961 ggtttcgagc gacaggccgt ccaagtgttg cggcatcagg ttttgcgtca ggcggatata
    16021 gttcaacagt gcacctgccg cgccaatcgc aacggcgtgt tctttgccat ccaaaccgaa
    16081 gccgcgcaaa tcctggcagc cgaaatattc cgtcagcagt ttttcccccg cgtcggcggc
    16141 aaactgccac gcgttcaggc gcgtaacacc cgatgccgtc tgaagttgcg gtgcgttttt
    16201 actgtcaggc aacagaattt ccgccgcctg caggcgcgcc agttcgtcgt ccaatttatc
    16261 cacagtcgtc agcttggttt tgaattcgcc gctttgcagc gatgcccacg ccaaaccgat
    16321 gtattttttg tcgggggaca cggcaacgat gcggttggtt tccttgtctt ccagcaatgc
    16381 ggaatcggtt agcgtgccag gcgttacgat gcgcacgact ttgcgttcca ccggcccttt
    16441 gcctgcgccg acttcgccga cctgttcgca aatcgccacg cttttgccca acttgaccag
    16501 gcgcgccaga tattgttcgg cggcgtgaaa cggcacgcct gccattttaa tcggtacgcc
    16561 gtccatctgc ccgcgcgtgg tcagggtaat atccaaaagt tttgccgctt ctaccgcatc
    16621 gtcgaaaaac atctcgtaaa aatcgcccat acggtaaaac accagtttgt cggtatgttg
    16681 cgctttgatg ccgaggtatt gctgcatcat tggggaaacg gcggatttgg acatggggct
    16741 gccttatcat taggaaaacg gtattgtagc aaaaggtttt gtcttcgacg gaatgagact
    16801 gaccgtaagc ctgtcaataa agttgtggag acaagtagga aaacagcaaa gttatccaca
    16861 ggaatattat taagtcaaaa aacatccacg tttatctgtc ctatacggca agccttaaac
    16921 tcaaacttat ttgacgaaaa ggtaatgaaa atattggata tttgcaactt atacacaagc
    16981 agatccgcaa tcatcaaaat catcattaaa attttaaatt caaatattga tggtaaaagt
    17041 tttggaaatc tgtgcaaaca gtaaaattca tctatatcaa ataagcatca agtagttaga
    17101 tgtaatagtg gaatgtaact ttatttcaga atacaataca gatattgaaa tttcgtttca
    17161 cagcttggag tatatacatg agcattaaag tagcgattaa cggtttcggc cgcatcggcc
    17221 gcctcgcatt gcgtcaaatt gaaaaagccc acggcatcga agtcgtcgct gtcaacgacc
    17281 tgacccctgc cgaaatgctg ctgcaccttt tcaaatacga cagcacgcaa ggccgtttcc
    17341 aaggtactgc cgaattaaaa gacgatgcca tcgtggtaaa cggcaaagaa atcaaagttt
    17401 tcgccaatcc gaatcccgaa gaattgcctt ggggcgagtt gggtgtggat gtcgtcctcg
    17461 aatgtaccgg cttctttacc aataaaacca aagccgaagc acacatcagg gccggcgcgc
    17521 gcaaagtcgt gatttccgca cccagcggca atgatgtgaa aaccgtcgta tatggcgtga
    17581 accaagacat tttggacggc agcgaaaccg ttatctccgc tgcttcctgc accaccaact
    17641 gccttgcccc gatggcggca gtcctgcaaa aggaattcgg cgtagtcgaa ggcctgatga
    17701 ccaccatcca cgcctacacc agcgaccaaa acacccttga cgcgccgcac cgcaaaggcg
    17761 atttgcgccg cgcccgcgcc gccgcgctca acatcgtgcc caacagcacc ggcgccgcca
    17821 aggccatcgg tctggttatc cctgaattga acggcaaact cgacggctcc gcccaacgcg
    17881 tccctgttgc caccggctcg ctgaccgaat tggtttccgt cctcgaacgc cctgtaacca
    17941 aagaagaaat caacgccgcg atgaaagcag ccgccagcga atcttacggc tacaacgaag
    18001 atcaaatcgt ttcttccgac gttgtcggca tcgaatacgg ctcgctcttc gatgcgaccc
    18061 aaacccgcgt gatgaccgtg ggcggcaaac aattggtgaa aaccgttgcc tggtacgaca
    18121 acgaaatgtc ctacacctgc caactcgtcc gcactttgga atactttgca ggcaaaatct
    18181 aaggcttgaa caaacctgtg gataagtaaa tgccgtctga acacattgcg ttcagacggc
    18241 attgtttatc tgtatagtgg attaaattta aaccagtacg gcgttacctc gccttgccgt
    18301 actatttgta ctgtctgcgg cttcgttgcc ttgtcctgat tta
//
LOCUS       DAXHTR010000023        18068 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.023, whole genome shotgun sequence.
ACCESSION   DAXHTR010000023 DAXHTR010000000
VERSION     DAXHTR010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18068
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.023"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(129..1232)
                     /gene="dnaN"
                     /locus_tag="ACFNML_000568"
     CDS             complement(129..1232)
                     /gene="dnaN"
                     /locus_tag="ACFNML_000568"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFC6391990.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPRAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERASILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(1467..3023)
                     /gene="dnaA"
                     /locus_tag="ACFNML_000569"
     CDS             complement(1467..3023)
                     /gene="dnaA"
                     /locus_tag="ACFNML_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFC6391991.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            3315..3449
                     /gene="rpmH"
                     /locus_tag="ACFNML_000570"
     CDS             3315..3449
                     /gene="rpmH"
                     /locus_tag="ACFNML_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003816025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFC6391992.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            3452..3817
                     /gene="rnpA"
                     /locus_tag="ACFNML_000571"
     CDS             3452..3817
                     /gene="rnpA"
                     /locus_tag="ACFNML_000571"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFC6391993.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLGH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVHRKFDRATAKQAR
                     AELAQLMFGNPATGCRKQA"
     gene            3882..4103
                     /gene="yidD"
                     /locus_tag="ACFNML_000572"
     CDS             3882..4103
                     /gene="yidD"
                     /locus_tag="ACFNML_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFC6391994.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            4275..5912
                     /gene="yidC"
                     /locus_tag="ACFNML_000573"
     CDS             4275..5912
                     /gene="yidC"
                     /locus_tag="ACFNML_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFC6391995.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDENKPFILFGDGKEYT
                     YVAQSELLDAQGNNILKGIGFSAPKKQYSLEGDKVEVRLSAPETRGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSTWILQPKGGQSVCAAGDCRIDI
                     KRRNDKLYSTSVSVPLAAIQNGAKAEASINLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMVAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRTQGEVVS"
     gene            complement(5993..6718)
                     /locus_tag="ACFNML_000574"
     CDS             complement(5993..6718)
                     /locus_tag="ACFNML_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HFC6391996.1"
                     /translation="MSEGMVSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAE
                     KTARAHLKHLGVTMPIRELNLQTLNEHTDLKTLPELLKPLQEGRSMGIVSEAGCPAVA
                     DPGANLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLK
                     ALEQRSRQQNETQLFIETPYRNDVLLADAVENLHPETRLCTATDLTLPTQSVVSKTVA
                     DWRRMKEMPNLKKRPTIFVMYAG"
     gene            complement(6765..7355)
                     /locus_tag="ACFNML_000575"
     CDS             complement(6765..7355)
                     /locus_tag="ACFNML_000575"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HFC6391997.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAHKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRKLDELHILRYLEREPDAVYCSCALKSEAL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            7503..8006
                     /locus_tag="ACFNML_000576"
     CDS             7503..8006
                     /locus_tag="ACFNML_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HFC6391998.1"
                     /translation="MSDPNLIDSEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSN"
     gene            8040..8219
                     /gene="rpmF"
                     /locus_tag="ACFNML_000577"
     CDS             8040..8219
                     /gene="rpmF"
                     /locus_tag="ACFNML_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011765354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFC6391999.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            8394..8921
                     /locus_tag="ACFNML_000578"
     CDS             8394..8921
                     /locus_tag="ACFNML_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HFC6392000.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            9009..10064
                     /gene="plsX"
                     /locus_tag="ACFNML_000579"
     CDS             9009..10064
                     /gene="plsX"
                     /locus_tag="ACFNML_000579"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFC6392001.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTTQVVGMDESPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            10200..10451
                     /locus_tag="ACFNML_000580"
     CDS             10200..10451
                     /locus_tag="ACFNML_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HFC6392002.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            10478..10893
                     /locus_tag="ACFNML_000581"
                     /pseudo
     CDS             10478..10893
                     /locus_tag="ACFNML_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            10913..11116
                     /locus_tag="ACFNML_000582"
     CDS             10913..11116
                     /locus_tag="ACFNML_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392003.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPVHGNLHLKTICIPNHTPI
                     PVSVICTLPVLGNHRFFAYNSRI"
     gene            11143..12105
                     /locus_tag="ACFNML_000583"
     CDS             11143..12105
                     /locus_tag="ACFNML_000583"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFC6392004.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDATGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGTVVLSASDKPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            12152..12523
                     /locus_tag="ACFNML_000584"
     CDS             12152..12523
                     /locus_tag="ACFNML_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HFC6392005.1"
                     /translation="MKRQAFFKPIACAAFLSAISLRLPVLGACYAILSLYAFALYGID
                     KRRAVRGKRRIPEHRLLLPALFGGWAGAYLGSRIFRHKTAKKRFVVLFRLTVSGNVLA
                     TLILIDYFVPPELFVKLGQHL"
     gene            12549..13475
                     /gene="fabD"
                     /locus_tag="ACFNML_000585"
     CDS             12549..13475
                     /gene="fabD"
                     /locus_tag="ACFNML_000585"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFC6392006.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFTGQAVVKNTFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LDFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVKQICAESTQGEVVEAVNFN
                     SPGQVVIAGNTAAVERAMLAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKAAACSALTDAGQVAAFIEAH"
     gene            13614..15479
                     /gene="msbA"
                     /locus_tag="ACFNML_000586"
     CDS             13614..15479
                     /gene="msbA"
                     /locus_tag="ACFNML_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HFC6392007.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSLIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAVNRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLAPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDSSPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            15579..17144
                     /gene="guaA"
                     /locus_tag="ACFNML_000587"
     CDS             15579..17144
                     /gene="guaA"
                     /locus_tag="ACFNML_000587"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFC6392008.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPVLGICYGMQFMAHHLGGEVQPGNQRE
                     FGYAQVKTIDSELTRDIFDDAPNTLDVWMSHGDKVSKLPDGFAVIGNTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAKEKKLTNAKWLAQGTIYPDVIES
                     AGAKTQKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            17206..17952
                     /gene="fabG"
                     /locus_tag="ACFNML_000588"
     CDS             17206..17952
                     /gene="fabG"
                     /locus_tag="ACFNML_000588"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFC6392009.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
BASE COUNT         4545 a         5485 c         4303 g         3735 t
ORIGIN      
        1 aaggcgaggc aacgccgtac tggtttttgt tcatccacta tacctttccg acagccgaac
       61 aaaaccccga atccgtctgc acggttcggg gtatatctcc aatacgggca tcgtgttccg
      121 gaaaaccgtc aaatccgcat cggcatcaca atatatttga aattcggatt gttcggcacg
      181 gtaaacagcg tcgagcggtt ggcatcgccg aaggcaagct gcatatcgtc ggaatggatg
      241 ttgcgcaaca cgtccatcag atagccgata ttgaaaccga cttcgagttc gccgccctga
      301 taggcgattt cgatttcttc gcgcgcttct tcctgctcgt tgttgctgca cacaacgctc
      361 aacaggccgg gttgcaaaaa caatcgcgca ccgcggaatt tttcattggc aagaatcgat
      421 gcacgttcca acgcgcccaa caattctgcc ctcgacaaca cgaaaatctt gtcgttgtcc
      481 aaaggaatca cgcggttgaa atcggggaat ttgccgtcga tgaccttgct gacgatggtc
      541 gtgccgttgc attggaaacg cacctgtttg tccagcagct cgatttgaat cggatcgtcg
      601 gggttgttca acagtttgaa cagttccagc accgttttgc gcggcaaaat cacttcggcg
      661 cgcggcaaat ccgcatcaat cgcgcaggct gcataggcaa ggcggtgtcc gtcggtcgcc
      721 acaaggcgca actggctgcc ctcaacctgc atcagcagac cgttgagata atagcggatg
      781 tcctgcaccg ccatgctgta ctgcacttgc gacagcatgg ttttgaaacg ctcctgctcc
      841 agcgagaaag tcgcgctgat gtcctcgccg acattcatca tcggaaaatc ggcggcaggc
      901 agggtctgca gggcaaaacg cgatttgccc gccttcagcg tcagacggct gtcgtcccaa
      961 tccagcgaca ccagcgcacc ggcaggcagc gcgcgcaaaa tgtcctgaaa tttcttggca
     1021 ttggtggtga tgcggaagtc gcccgcgccg ccctcgggac ccgcagtgtc gatttggatt
     1081 tccaaatcgg ttgccaagag tttggtctga ccgccttttc cctcaatcag gacgttggac
     1141 aggatgggca gggtgtggcg gcgttcgaca atgccggtaa cggcttgcaa cggcttgagc
     1201 aggctgtcgc gctcggcttg taaaatcaac atgttcgctc ctttaaatcg gtttgtatag
     1261 tggattaaat ttaaatcagg acaaggcgac gaagccgcag acggtacaaa tagtacggaa
     1321 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
     1381 acgccgtact ggtttaaagt taatccgcta tatctttacc cttcggacgg catgggcaat
     1441 atcatgtcgt ctgaaaacgt tttccatcag ttttgaatca gaatcagcag cttttcataa
     1501 tcctgagcca attccggatc ttcttcgcgc agtttcgcca ctgccctgat gccgtgcata
     1561 acggtcgtat ggtcgcgccc accaaacgaa tcgccgatag acggcaggct caaagtagtc
     1621 agttctttgg tcaggctcat cgccacctgg cgcggacggg caatgtttcg tgtccgtttc
     1681 ttaccgagca catcgctgat tttgatgcgg taatatttcg ccaccgcatc gatgatgatg
     1741 tcggcggtga tgactttgtg cttctcggca ataatgtcct gcaaagcggt acgcgccaaa
     1801 tcgatgtcga tgacgggacg gttcataaag cggctgctcg ctccgacacg attaaacgcg
     1861 ccttcaagct cgcgcacgtt ggaacggatc agattggcaa tgaacagcgc ggcttcgtct
     1921 tcgatactga tgcccgccgc ttccgccttt ttctgcaaaa tggcgatgcg catttccaat
     1981 tcgggcggct cgagttccaa agtcagtccc catgaaaaac gggatttgag gcggtcgtcc
     2041 atgccttcga ttttcgcagg caacacatcg caagtgagga tgagctgttt tttctcgttg
     2101 tggaaatggt tgtacagata gaaaaactct tccatcgtac ggtctttgcc tttgatgaac
     2161 tggatgtcgt cgataatcag caggtcgtat tgcttgtatt gctgcttgaa cacgtcgtaa
     2221 gtgttgttgc gaaccgcctt cataaagctg cggatatagt catccgaatg catatagcgc
     2281 actttggcat cgggacggtt tttcagcagc tcgttgccga ccgcctgcac aaggtgggtt
     2341 ttgcccaaac ccgtactgcc atagaggaag aacgggttgt aactctgccc cgggctttcc
     2401 gcaatcgcct gcgccgcagc cgccgcaagg cggttgccct taccttctac caacgtatca
     2461 aacgtgtaat ccggagacag gttggtctgc tcgtaacgcg cctcttccgc atcgcgctgc
     2521 acgtccgtcc gtgctttggc aactgccacc gattccggcc gggaagcaga cccggcagcc
     2581 tgacgcggct cgtgcggcag gtttttcata cgttccgcca aaatatccgc cgccgttttc
     2641 gacgcagcgg gtttgacagg ctcttcagac ggcagctcgt ccaacagaac ctcctgcacg
     2701 ggcattccct ccgacaccgc atgcaaggac ggctcggcag gttcgacagc accttcaacc
     2761 gccgccatct cataacgcac gccttctccc ggtttgaata cgaaggcgga acggccggca
     2821 gccaactctt ccctcaccgc ttctattttt ccggcaaact ggctcttgag catattgcag
     2881 gcaaactggt tcttgccgta caccacccat acgccaccct cctcaccaac ggtaaggggc
     2941 gcaatccatt gcgcaaactg cccttgaggc aacatatcgt gaagacggcg gaggcacagc
     3001 ggccaaaact ctgctaatgt catggataag ctcgaatcgg taaaaatgaa atcgaaaaca
     3061 aagaaaatat aatattttca aaaagaaaac aaatctgttg aacgcacatc ggttcaaaac
     3121 gcgactgccc gattataccg actcacgaat attttatcca caacccgtgc aaaaatttat
     3181 ccacagaaag gcggcggaaa tccgcaggca atcgggcaat cttcctgcaa agtttctata
     3241 ttgattgaca aaagcggcaa attggagtgt aattcacggt ttaattatct acccattcta
     3301 ttttaggaaa catcatgaaa cgcacttatc aaccttccgt taccaaacgc aaacgcaccc
     3361 acggcttcct ggtgcgctcc aaaacgcgcg gcggccgcgc agtattggcc gcacgccgtg
     3421 ccaaaggccg caaacgcctg gcggtataat tttggactac cgcttcggaa ggcagtaccg
     3481 cttgttgaaa acggatgatt tttcatccgt ttttgcattc agaaaccgcc gcagccgcga
     3541 cctgctgcaa gtttcgcgct caaacggcaa cgggctgggc catccccgca tcggtctggt
     3601 ggtcggcaaa aaaaccgcca aacgcgccaa cgaacgaaat tatatgaagc gcgttatccg
     3661 cgactggttt agattgaaca aaaaccggct gccgccgcag gatttcgtcg tgcgcgtcca
     3721 ccgtaaattc gacagggcta ccgcaaaaca ggcaagggcg gaactggcac aactcatgtt
     3781 cggcaacccc gcaaccggat gcaggaaaca ggcatgatca gaacggtact ctgcaggcaa
     3841 ggttcagacg gcaacgggtt tcccatacaa ggaacatccc gatgaacttc ctattgtcca
     3901 aactcctgct gggactgata cggttctacc aatattgcat cagcccgctg attccgccgc
     3961 gctgccgtta tacgccgacc tgttcgcaat acgcggtcga agcggtcaaa aaatacggcg
     4021 cattcaaagg cggccggctc gccatcaagc gcattgcacg ctgccaccct ttcggcggac
     4081 acggacacga ccccgttccc tgacccgacg caatattcaa attgcacgct ttccttttat
     4141 ttcccatcgg tttctatata atgccgtctg aagcttcggg caggcggcac gaccgccggg
     4201 tatgaagccc gcccttattc cccgtctatc ggaacacgca acctgcggca tttccgacca
     4261 ttcaggaaac tcttatggat tttaaaagac tcacggcgtt tttcgccatc gcgctggtga
     4321 ttatgatcgg ctgggaaaag atgttcccca ctccgaagcc cgtccccgcg ccccaacagg
     4381 cagcacaaca acaggccgta accgcttccg ccgaagccgc gctcgcgccc gcaacgccga
     4441 ttaccgtaac gaccgacacg gttcaagccg tcattgatga aaaaagcggc gacctgcgcc
     4501 ggctgaccct gctcaaatac aaagcaaccg gcgacgaaaa taaaccgttc atcctgtttg
     4561 gcgacggcaa agaatacacc tacgtcgccc aatccgaact tttggacgcg cagggcaaca
     4621 acattctaaa aggcatcggc tttagcgcac cgaaaaaaca gtacagcttg gaaggcgaca
     4681 aagttgaagt ccgcctgagc gcgcctgaaa cacgcggtct gaaaatcgac aaagtttata
     4741 ctttcaccaa aggcagctat ctggtcaacg tccgcttcga catcgccaac ggcagcggtc
     4801 aaaccgccaa cctgagcgcg gactaccgca tcgtccgcga ccacagcgaa cccgagggtc
     4861 aaggctactt tacccactct tacgtcggcc ctgttgttta tacccctgaa ggcaacttcc
     4921 aaaaagtcag cttctccgac ttggacgacg atgccaaatc cggcaaatcc gaggccgaat
     4981 acatccgcaa aaccccgacc ggctggctcg gcatgattga acaccacttc atgtccacct
     5041 ggatcctcca acccaaaggc ggacaaagcg tttgcgccgc tggcgactgc cgtatcgaca
     5101 tcaaacgccg caacgacaag ctgtacagca ccagcgtcag cgtgccttta gccgccatcc
     5161 aaaacggcgc gaaagccgaa gcctccatca acctctacgc cggcccacag accacatccg
     5221 ttatcgcaaa catcgccgac aacctgcaac tggccaaaga ctacggcaaa gtacactggt
     5281 tcgcttcccc gctcttctgg ctcctgaacc aactgcacaa catcatcggc aactggggct
     5341 gggcgattat cgttttaacc atcatcgtca aagccgtact gtatccattg accaacgcgt
     5401 cctaccgttc gatggcgaaa atgcgtgccg ccgcgcccaa actgcaggca atcaaagaga
     5461 aatacggcga tgaccgcatg gcgcaacaac aggcgatgat gcagctttac acagacgaga
     5521 aaatcaaccc gctgggcggc tgcctgccta tgctgttgca aatccccgtc ttcatcggat
     5581 tgtattgggc attgttcgcc tccgtagaat tgcgccaggc accttggctg ggttggatta
     5641 ccgacctcag ccgcgccgac ccctactaca tcctgcccat cattatggcg gcaacgatgg
     5701 tcgcccaaac ttatctgaac ccgccgccga ccgacccgat gcaggcgaaa atgatgaaaa
     5761 tcatgccgtt ggttttctcc gtcatgttct tcttcttccc tgccggtctg gtattgtact
     5821 gggtagtcaa caacctcctg accatcgccc agcaatggca catcaaccgc agcatcgaaa
     5881 aacaacgcac ccaaggcgaa gtcgtttcct aaatgccgca gcatgaaaaa tgccgtctga
     5941 aacctgttca gacggcattt ttattgttca ccccctatcg gggcggaaat cttcaacctg
     6001 catacatcac aaaaatcgtc gggcgttttt tcagattggg catttccttc atcctgcgcc
     6061 aatccgcaac cgttttactg accaccgact gagtcggcaa cgtcaaatcc gtagccgtgc
     6121 acaagcgcgt ttcaggatgc aggttttcca ccgcatccgc caacaacaca tcgttgcgat
     6181 acggcgtttc gataaacagc tgcgtttcat tctgctggcg cgaacgttgt tccaaagcct
     6241 tcaaactctg aatccgctca tttttttcag acggcaaata acccttaaac gcaaagttct
     6301 gcccattcgc acccgaagcc atcagcgcca gcaacaagct ggacggaccg accaaaggcc
     6361 gtacttcaaa accgtgttta tgcgccaatg ccaccaaatt cgcacccgga tcagccacag
     6421 ccgggcaacc cgcctcgctg acaatgccca tactgcgccc ttcttgcaaa ggtttcagca
     6481 attccggcaa agtcttcaaa tccgtgtgtt cgttcagcgt ttgcaaattc agttcgcgga
     6541 taggcattgt cacgcccaaa tgtttcaaat gcgcgcgcgc cgttttttcc gcttccacga
     6601 caaaatccgt cagcccgaca atcgcctgtt gttcatgcgg caacaggcac ggcgtgtcag
     6661 gcgtacccaa aggcgtagga atcaaataca aaacaggaga caccattccc tcactcatcg
     6721 gttaaaaatg ccgtctgaac ccttcagacg gcataaacgg gcagttacag aacctccacg
     6781 ccctcatttt tcaaaaaatc aaccagacgg aaaaccggca aaccgattaa agcattcgga
     6841 tcggtactct caatcctttc gatcaacaat gcacccaaag cctcgctctt caacgcacac
     6901 gagcaataaa ccgcatcagg ctcgcgctcc aaatagcgga ggatgtgtag ctcgtccaac
     6961 ttcctcatca cgaccaccgt cttatcgata tgccgctgca tcctgcccgt aaccgtattt
     7021 agcagaacga ccgcgctgta aaactcaatc tccctgccgc tcaaatgcat caacatcttg
     7081 tgcgcattag caaggttcat cggcttgccc cactgcctgc cgtcgcacca cgccacctgg
     7141 tccgcaccga caatcaacgc ctctgggaaa cgcccggcca acgaccgcgc cttaccctcg
     7201 gcaaggcgca atgccgtctg aggggcggat tcccccaaca tcggcgtttc gtcaaaatcg
     7261 ggggacgccg cctgaaaggc aatgccgagc ctttccatct gttcgcggcg gaaaaccgaa
     7321 ctcgtaccca aaatcaaagg cagttccaaa cccatcccat cctccttacc gttgaaaaca
     7381 cgcccgaagg ggcagtaaaa tccagccatg cgccgaaaca cggatacccg ccttcggcgt
     7441 accgcaacat ttttcttaaa aatattgacg ttagaacatc taaattatat catatcccgt
     7501 ttatgtcaga ccctaatttg attgactcgg aaatttttgc cgccgaaggg cagaacctgc
     7561 aaggcagttt tctgctggaa gaattggatg aacgcgtcag ttcgcacgat tatcccgccg
     7621 acaggcagac caaaatatcg tttacactga ccggcggtcg cgaccggctg caacgcctgt
     7681 tcctcgacct gaacgtcaaa gccgatatgc ccctgatttg ccagagatgt atcaaaccca
     7741 tgccgttcat gcttgatgaa agcagccgta tcgtcctgtt ttccaacgaa gagtccttgg
     7801 acgaatccat gcttgccgac gaagaactcg aaggcatact gattgaaaaa gaactcgacg
     7861 tgcgcacatt ggtagaagac caaatcctga tgtccctgcc cttttcgccg cgacacgaag
     7921 actgcggcga caatgggaca ctggaagaag tcaatcggga caaacccaac ccctttgctg
     7981 ttttggcagg tttgaaaagc aattgattag gacacagttt atttatctag gagcttgaaa
     8041 tggccgttca acaaaacaaa aaatcccctt ccaaacgcgg tatgcaccgt tcgcacgacg
     8101 cgctgaccgc gcccgcactg tctgtcgaca gcacaaccgg cgaagtacac cgcccgcacc
     8161 acatttcccc caacggtatg taccgcggcc gcaaagtggt caaagccaaa ggcgaataat
     8221 ccctattcga ctgactgaaa aagccagaac attgccatgc aattactggc tttttttgca
     8281 ttggacgcac catccgtcca aactttcgcc atacgtcaac acacaggggc aaagcgttcc
     8341 gtataataac ccgtgaaaat attccaaaag ccccaaccac caaggaaatt ccgatgaaac
     8401 agaaaatctg gtacacctac gatgacatcc accgcgtcat caaagcattg gcagaaaaaa
     8461 tccggaacgc cgacatcaaa tacgatgcca tgattgccat cggcggcggc ggctttattc
     8521 cggcacgtat gctgcgctgt tttctggaaa ttccgattta tgccgtaacc accgcctatt
     8581 acgacagcga caacgaagga caggttacag aagaagtcaa aaaagtccaa tggctcgacc
     8641 ccgttcccga agccctgcgg ggcaaaaacg tactcgtcgt cgatgaagtg gacgacagcc
     8701 gcgtaaccat ggaattttgc ctgacagagc tgctcaaaga agacttcggt acgatcggcg
     8761 tcgccgtact gcacgaaaaa atcaaagcca aagcaggcaa aatccccgaa ggcattccct
     8821 atttcagcgg catcaccgta gaagactggt ggatcaacta tccgtgggac gcactcgaca
     8881 tcgacgaaca caaccgcctt gccgaggccg gccgaggctg accctttcag acggcatatt
     8941 ttccgaaccg atgccgtctg aagcccgcac gacccctgcc gcagaccgaa aacctaccgg
     9001 agaaccctat gattacactt gccgtagatg ccatgggcgg cgaccaagga cttgccgtta
     9061 ccgtacccgg cgcaaccgca ttcctccaag cacaccccga tgtccgcctg attatgaccg
     9121 gcgacgaaac gcaactgcgc caagccctga ccgcggcagg cgcaccgatg gaacgcatcg
     9181 acatctgcca taccacccaa gttgtcggca tggatgaatc ccctcagtcc gccctaaaga
     9241 acaaaaaaga ctcctccatg cgcgttgcca tcaaccaagt taaagaaggc aaagcccaag
     9301 ccgccgtatc cgcaggcaac acgggcgcac tcatggcaac cgcacgtttc gtcctcaaaa
     9361 ccattcccgg catcgaacgc cccgccatcg ccaaattcct tccttccgac accgaccacg
     9421 ttaccctcgc cctcgacctc ggcgcaaacg tcgactgcac gcccgaacag ctcgcccaat
     9481 ttgccgttat cggcagcgaa ctcgtccacg cactccatcc tcaaaaagga cagccgcgtg
     9541 tcgggctggt caacgtcggc acggaagaca tcaaaggtac ggacaccgtc aaacaaacct
     9601 acaaactgct gcaaaacagc aaactcaact ttatcggcaa catcgaaagc aacggcatcc
     9661 tgtacggcga agcagatgtc gtcgtcgccg acggctttgt cggcaacgtc atgctcaaaa
     9721 ccatcgaagg cgcggtcaaa ttcatgagcg gagccatccg ccgcgaattc caaagcaacc
     9781 tgttcaacaa acttgccgcc gttgccgccc tacccgccct caaagggctg aaaaacaaac
     9841 tcgatccgcg caaattcaac ggggccatcc tgctcgggct gcgcggcatc gtcatcaaaa
     9901 gccacggcgg cacagacgaa accggtttcc gctacgcact cgaagaagcc taccacgaag
     9961 ccaagtccgc cggcctttcc aaaatcgaac aaggcgttgc cgaacaactc gccgcactcg
    10021 aaactgccaa agccgtccaa aacgaaaatg ccgacggtct gtaacacaca cgatgccgtc
    10081 tgaacgcccc cgcccctttc agacggcatc cgcccgcacc aaacctgcgg gcgcggacgg
    10141 cgatgcgcct gtccggcact tcccaaatac ttccctgtaa aataaggagt atttgaaaaa
    10201 tgaagacatt agaaaaacgg atgaaagctc tagacaaaca gatgatgaag ttcggaaaat
    10261 cccttgaagg caggcttgat gcccgtctga ttgaatccgc attggattat attcattatt
    10321 cggaacgttt tttggctttt gaaatcctgt gtacttatat cgaagatttc gatgtccggc
    10381 tgacggaaca agaatcccgg gaaatttctt ttatcaacaa ggaatttgag atagaaagca
    10441 cgtccgatta accaataaag ccaatgggtt gataaacatg aaaacatcga cggtcgtttt
    10501 tggcggattt tttatggcag acaacggaga gcgaatccaa atccccgttt tggaaaatcc
    10561 tgacattagg gaaatcaatc acttttttcc gtatcaaatt ttgagaaaaa agccggcgtc
    10621 cttgttttca gaatcatccc cgagccggaa tttggcgata ccgaattaac tatctatttt
    10681 gaaaaaggat attatttgcc aatgattcaa acaattttag aagacgggga tattgaagtc
    10741 aaaaacctta aaacagaaaa ttacagcggg aaggcaaggg aaatcttggg agattcctac
    10801 cctattgagc atatctctaa aaatatttct acaattcaaa atataatttc tgaatttatg
    10861 atacaaaaac aaacaccggc accaataatt tgatggaaaa ccaacaatca aaatgccgtc
    10921 tgaagcatgt ttggttttca gacggcattt ccttcgctta aaacagcgta tcggcaaccc
    10981 cgccctgcct gtccacggca atctgcatct gaaaaccatc tgtatcccaa accacacccc
    11041 catccctgtt tccgtcattt gcaccctgcc cgtattgggc aatcatcggt ttttcgctta
    11101 caatagccga atctgaacca actctctaaa aaggccgttc ccatgcagta tgcaaaaatt
    11161 tccggcacgg gcagctatct tcccgccaac cgcgtcagca atgacgacct tgcccaaaag
    11221 gtagatacct ctgacgagtg gattaccgcg cgtacgggca tcaagttccg ccatattgca
    11281 gccgaaaatg agaaaaccag cgatcttgcc gccgaagcag cccgccgcgc actggatgca
    11341 accggattag acagcggcga aatcgattta attattgtgg cgacggcaac gccggatatg
    11401 cagtttcctt ctaccgccac catcgtccaa caaaaactgg gcatcaccaa cggctgcccc
    11461 gcgtttgacg tacaggcggt gtgtgcgggc tttatgtacg cgctgaccac ggcaaacgcc
    11521 tacatcaaaa gcggcatggc aaaaaacgcg ctggtcatcg gcgcggaaac cttcagtcgc
    11581 attgtagact ggaacgaccg cacaacctgc gtattgttcg gcgacggtgc gggcacggtg
    11641 gttttaagcg cgtccgacaa accgggcatc atccacagca aactcaaagc cgacggcaat
    11701 tatctgaaac tcttaaacgt ccccgggcaa atcgcctgcg gcaaagtttc cggttcgccg
    11761 tacatttcga tggacggtcc cggcgtgttc aagtttgccg tcaaaatgct gtccaaaatc
    11821 gccgatgacg ttatcgaaga agccggttat accgccgctc aaatcgactg gatcgtgccg
    11881 catcaggcaa accgccgcat tatcgaatcg accgcgaaac atttaggttt gagtatggac
    11941 aaagtcgtcc tgaccgtcca agaccacggc aacacatccg ccgcatcgat tccgctggct
    12001 ttggatacgg gcatccgcag cggacaaatc aaacgcggtc aaaacctgct gctcgaaggc
    12061 atcggcggcg gtttcgcgtg gggcgcggta ttgctgcaat attgaatacc atgccgtctg
    12121 aagcaggttt tcagacggta tttcccatat catgaagcgg caggctttct tcaaaccaat
    12181 tgcgtgtgcg gcatttctgt ctgccatttc gctgcgcctc cccgtattgg gcgcgtgtta
    12241 cgcaatattg tccctctatg cgtttgcact ttacggcatc gacaaacggc gtgccgtgcg
    12301 gggaaaacgc cgcattcccg aacaccgcct gctgctgcct gccttgttcg gcggttgggc
    12361 gggcgcatac ttgggcagca ggatattcag gcataaaacg gcgaaaaagc gttttgttgt
    12421 gctgttccgt ctgactgttt caggtaatgt cctggcgacc ctcatcctga ttgattattt
    12481 cgttccgccc gaactttttg tcaaactcgg gcaacatctc tgacaatcca accaaacaaa
    12541 ggaataccat gtcttttgca ttcttctttc ccggacaagg ttctcaaagc ctcggcatga
    12601 tgaacggctt tacagggcag gccgtcgtca aaaacacatt tgacgaagca tccgccatat
    12661 tgggtcaaga cttgtgggcg atgattaacg gtagcgatgc cgaaatcatc ggtcaaaccg
    12721 tcaataccca acccatcatg ctcgccgccg gcgttgccgt ttaccgcgcc tatttggaag
    12781 cgggcggcaa aacgcctgcc gccgttgccg gacacagcct cggcgaatat accgcactcg
    12841 ttgccgccgg cgcattggat tttgccgacg cggtcaaact cgtgcgcctg cgcgccgaac
    12901 tgatgcagtc tgccgtaccg caaggcgtgg gcgcaatggc ggcgattctc ggcttggaag
    12961 atgaacaggt aaaacaaatt tgcgccgaat ccacacaagg cgaagtggtt gaagccgtca
    13021 acttcaactc gcccggacaa gtcgtgattg caggcaatac cgccgccgtc gagcgcgcta
    13081 tgcttgccgc caaagaagcc ggcgccaaac gcgccctgcc gctgcccgtg tccgtacctt
    13141 cccattgcag cctgatgaaa cccgccgccg acaaactcgc cgaagccctg aaaaccgttg
    13201 aaatcaagca gccgcaaatc cgcgtcatcc acaacgccga cgttgccgcc tacgatgatg
    13261 ccgacaaaat caaagacgcg ctcgtccgcc agctttacag ccccgtacgc tggacagaaa
    13321 ccgtcaacgc ccttgtttca gacggcattg ccgaatccgc cgaatgcggc ccgggcaaag
    13381 tgttggcggg cttggcaaaa cgcatcaaca aggccgccgc gtgcagcgca ctgaccgatg
    13441 ccggacaggt tgccgccttt atcgaagcgc actgacttcg ttccgcaaaa agcagcctgc
    13501 cctcttcagg ctgcttttca cgtccgaacg gcaaacagcc gcatatttgc gctataattg
    13561 atgacgacca aaccaccgac agcggctgcc gttgcagttc ccgccctacc gatatgatag
    13621 aaaaactgac tttcggactg tttaaaaaag aagacgcgcg cagctttatg cgcctgatgg
    13681 cgtacgtccg cccctacaaa atccgcatcg ttgccgccct gattgccatt ttcggcgttg
    13741 ccgccaccga aagctacctt gccgccttca tcgcccccct gattaaccac ggcttttccg
    13801 cacctgccgc gccgcccgag ctgtctgccg ccgccggcat catttccacc ctgcaaaact
    13861 ggcgcgaaca gtttacctat atggtttggg ggacggaaaa caaaatctgg accgtccccc
    13921 tcttcctcat catcctcgtc gtcatccgtg gcatctgccg ctttaccagc acctatctga
    13981 tgacttgggt ctccgtgatg accatcagca aaatccgcaa agatatgttt gccaaaatgc
    14041 tgaccctttc ctcccgctac catcaggaaa cgccgtccgg caccgtactg atgaatatgc
    14101 tcaacctgac cgaacagtcg gtcagtaacg ccagcgacat cttcaccgtc ctcacgcgcg
    14161 acacgatgat cgttaccggc ctgaccatcg tcctgcttta cctcaactgg cagctcagcc
    14221 tcatcgttgt cctgatgttc cccctgctct ccctgctctc gcgctactac cgcgaccgtc
    14281 tgaagcacgt catttccgac tcgcaaaaaa gcataggcac gatgaacaac gtgattgccg
    14341 aaacccatca gggacaccgc gtcgtcaagc tgttcaacgg gcaggcgcag gcggcaaacc
    14401 ggtttgacgc ggtcaaccgc accatcgtcc gcctcagcaa aaaaatcacg caggcaacgg
    14461 cggcacattc cccgttcagc gaactgattg cctcgattgc cctcgccgtt gtcatcttca
    14521 tcgccctgtg gcaaagccaa aacggctaca ccaccatcgg cgaatttatg gcattcatcg
    14581 tcgcgatgct gcaaatgtac gcacccatca aaagccttgc caacatcagc atccctatgc
    14641 agacgatgtt cctcgccgcc gacggcgtat gtgcatttct cgacaccccg cccgaacagg
    14701 acaaaggcac gctcgcgccg cagcgtgtcg aagggcgtat cagcttccgc aacgtcgatg
    14761 tcgaataccg ttcggacggc atcaaagccc tcgacaactt caacctcgac atcagacaag
    14821 gcgaacgcgt cgccctggtc ggacgttccg gcagcggcaa atccaccgtc gtcaacctgc
    14881 tgccccgctt tgtcgaaccg tctgccggca acatctgcat agacggtatc gacatcgccg
    14941 acatcaaact cgactgcctg cgcgcccaat tcgccctcgt ctcccaagac gtattcctgt
    15001 ttgacgacac cctgtttgaa aacgtccgat acagccgtcc cgacgcaggc gaagccgaag
    15061 tcctgtccgc cctccaagcc gccaacctgc aaagcctgat tgacagctcc ccgctcggac
    15121 tgcaccagcc catcggatcg aacggcagca acttgtccgg cggacagcgg caacgcgtcg
    15181 ccatcgcccg cgccattttg aaagacgcgc cgatattatt attggacgaa gccaccagcg
    15241 cattagacaa cgaatccgaa cgcctcgtcc aacaagcgct cgaacgcctg atggaaaacc
    15301 gcaccggcat catcgtcgcc caccgcctga ccaccatcga aggggccgac cgcatcatcg
    15361 taatggacga cggcaaaatc atcgaacaag gcacacacga acaactgatg tcccaaaacg
    15421 gctactacac gatgttacgc aatatctcaa acaaagatgc cgccgtccgg acggcataaa
    15481 caaaatgccg tccgaaatgg tataatcgcc ccgacccttt cagacggcac tatatccgcc
    15541 gacccatccg attatcttca atcactgtaa aacccattat gacccaagac aaaatcctca
    15601 tcctcgactt cggttcgcaa gttacccagc tgattgcccg ccgcgtgcgc gaagcccacg
    15661 tttactgcga actgcattcc ttcgatatgc ctttggacga aatcaaagcc ttcaacccca
    15721 aaggcatcat cctttccggc ggccctaatt ccgtttacga atccgactat caggccgata
    15781 ccggtatttt tgatttgggc attccggttt tgggcatctg ctacggaatg cagtttatgg
    15841 cgcaccactt gggcggcgaa gtgcagcccg gcaaccagcg cgaattcggt tacgcgcaag
    15901 tcaaaaccat cgacagcgaa ctgacccgcg atattttcga cgacgcgccc aacacactcg
    15961 acgtatggat gagccacggc gacaaagtgt ccaaactgcc cgacggcttc gccgtcatcg
    16021 gcaacacccc gtcctgcccg attgccatga tggaaaacgc cgaaaaacaa ttctacggca
    16081 tccagttcca ccccgaagtt acccacacca aacaaggccg cgccctgttg aaccgctttg
    16141 tcttggatat ttgcggcgca caaccgggct ggacgatgcc gaactacatc gaagaagccg
    16201 ttgccaaaat ccgcgaacag gtcggcagcg acgaagtgat tttaggtttg tcaggcggcg
    16261 tagactcttc cgtagccgcc gcgctgattc accgcgccat cggcgaccaa ctgacctgcg
    16321 tgttcgtcga tcacggtttg ttgcgcctga acgaaggcaa aatggtgatg gatatgttcg
    16381 cccgcaactt gggtgtgaaa gtgattcacg tcgatgccga agggcagttt atggcgaaac
    16441 tcgccggcgt taccgaccct gagaaaaaac gcaaaatcat cggcgcggaa tttatcgaag
    16501 tatttgatgc caaagagaaa aaactcacca acgccaaatg gctggcgcaa ggcacgattt
    16561 accccgacgt aatcgaatcc gcaggcgcga aaacccaaaa agcccacgcc atcaaatcgc
    16621 accacaacgt cggcggtctg cccgaaaata tgaagctcaa gctgcttgag ccgttgcgcg
    16681 acttgtttaa agacgaagtg cgcgaactgg gcgttgcttt gggtctgccg cgcgaaatgg
    16741 tgtaccgcca tccgttcccc ggccccggtt tgggcgtgcg catcttgggc gaagtgaaaa
    16801 aagaatacgc cgacttattg cgtcaagcag acgatatttt tattcaagaa ttacgcaaca
    16861 ccaccgacga aaacggcacg tcttggtacg acctgaccag ccaagccttc gccgtgttcc
    16921 tgcctgtgaa atccgtcggc gtaatgggcg acggacgcac ttacgactac gtcgtcgcac
    16981 tgcgcgcagt catcaccagc gactttatga ccgcacattg ggctgagttg ccttactcac
    17041 tgctcggacg cgtgtccaac cgcatcatca acgaagtcaa aggcatcaac cgcgtggtgt
    17101 acgatgtcag cggcaaaccg cctgccacca tcgagtggga ataaccagca aacagggctg
    17161 caccgtccgg cgcagtcttt cgattatcgg aaaaaaggaa aaaatatgag cacacaagat
    17221 ttaagcggca aaatcgcttt ggtaacaggc gcctcgcgcg gtatcggtgc agcaattgcc
    17281 gacacactgg cggcagccgg tgccaaagtc atcggtacgg cgaccagtga gagcggtgcg
    17341 gcggcgatta gcgagcggtt ggcgcaatgg ggcggcgaag gccgcgtatt aaattccgcc
    17401 gaacccgaaa ccgtcgaaaa cctgattgcc gacatcgaaa aaacgttcgg caaactcgat
    17461 attctggtca acaacgccgg catcacccgc gacaacctcc tgatgcgtat gaaagaagaa
    17521 gagtgggacg acatcatgca ggtcaacctc aaatccgtct ttcgcgcctc caaagccgtc
    17581 ttgcgcggca tgatgaagca gcgcgccggc cgtatcatca acatcacatc cgtcgtcggc
    17641 gtgatgggca atgccggtca aaccaactat gccgcggcaa aagcaggctt aatcggtttc
    17701 tccaaatcca tggcgcgcga agtcggcagc cggggcatta ccgtcaactg cgtcgccccc
    17761 ggctttatcg ataccgacat gacccgcgcc ctgccggaag aaacccgcca aacctttacc
    17821 gcccaaaccg ccttgggcag attcggcgac gcgcaagaca ttgccgatgc agtcttgttc
    17881 ttggcttccg atcaggcaaa atacattacc ggccagacgc tgcacgtcaa cggcggcatg
    17941 ctgatgccct gacggacagc cgtgccgaca atgccgtctg aagccctttc agacggcatt
    18001 tgcattccca aacaaaatga acacacgcca caccccgccc tgcccctgcg gatcaggcac
    18061 aagctgag
//
LOCUS       DAXHTR010000024        18038 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.024, whole genome shotgun sequence.
ACCESSION   DAXHTR010000024 DAXHTR010000000
VERSION     DAXHTR010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18038
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.024"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(259..561)
                     /locus_tag="ACFNML_000589"
     CDS             complement(259..561)
                     /locus_tag="ACFNML_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HFC6392010.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
     gene            complement(533..820)
                     /locus_tag="ACFNML_000590"
     CDS             complement(533..820)
                     /locus_tag="ACFNML_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HFC6392011.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            900..1505
                     /locus_tag="ACFNML_000591"
                     /pseudo
     CDS             900..1505
                     /locus_tag="ACFNML_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1484..1780
                     /locus_tag="ACFNML_000592"
     CDS             1484..1780
                     /locus_tag="ACFNML_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HFC6392012.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTRWKVLYTLINGVFFDRQHCRQAYISELKGRQHDARFNQSRAAGEKGTR"
     gene            1777..1986
                     /locus_tag="ACFNML_000593"
     CDS             1777..1986
                     /locus_tag="ACFNML_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392013.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPTYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(2156..3238)
                     /locus_tag="ACFNML_000594"
                     /pseudo
     CDS             complement(2156..3238)
                     /locus_tag="ACFNML_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003743106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SbmA/BacA-like family transporter"
     gene            complement(3681..4328)
                     /locus_tag="ACFNML_000595"
     CDS             complement(3681..4328)
                     /locus_tag="ACFNML_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HFC6392014.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKQNGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
     gene            complement(4325..4693)
                     /locus_tag="ACFNML_000596"
     CDS             complement(4325..4693)
                     /locus_tag="ACFNML_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HFC6392015.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            complement(4703..5374)
                     /locus_tag="ACFNML_000597"
     CDS             complement(4703..5374)
                     /locus_tag="ACFNML_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HFC6392016.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVEKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            complement(5539..6258)
                     /locus_tag="ACFNML_000598"
     CDS             complement(5539..6258)
                     /locus_tag="ACFNML_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6392017.1"
                     /translation="MATLSDKTILITGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            complement(6342..7655)
                     /gene="hpnE"
                     /locus_tag="ACFNML_000599"
     CDS             complement(6342..7655)
                     /gene="hpnE"
                     /locus_tag="ACFNML_000599"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HFC6392018.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARYADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRALPLPAPLHILGGVLLARRVPSAFKGKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNPLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTAQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFTNRAWADKVHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLSDAV"
     gene            complement(7685..7891)
                     /locus_tag="ACFNML_000600"
                     /pseudo
     CDS             complement(7685..7891)
                     /locus_tag="ACFNML_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224552.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8053..8925)
                     /gene="hpnD"
                     /locus_tag="ACFNML_000601"
     CDS             complement(8053..8925)
                     /gene="hpnD"
                     /locus_tag="ACFNML_000601"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HFC6392019.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTVLYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGCLIARILGFSDDQTLEYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMRRFDVPASVILQCSPTGNFAELMAFQIKRARETYCE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            9096..10958
                     /gene="hscA"
                     /locus_tag="ACFNML_000602"
     CDS             9096..10958
                     /gene="hscA"
                     /locus_tag="ACFNML_000602"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFC6392020.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRVTLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGGNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMPIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLNNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVS
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVQAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLEAEELQQI
                     RQGIADLQGRVKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            11034..11681
                     /locus_tag="ACFNML_000603"
     CDS             11034..11681
                     /locus_tag="ACFNML_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6392021.1"
                     /translation="MEKSGIVYLMKTVIKGVYKIGISDVSNFEGRMRHLENNGYANVA
                     GLERILAVKTDNYKEKENLLHEIFSKSRTELFAVDENLVKRLFLSLRGEIVFPKNETA
                     ESEFEKSVHERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRL
                     FKEAYLRKSGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPR"
     gene            11717..12478
                     /locus_tag="ACFNML_000604"
     CDS             11717..12478
                     /locus_tag="ACFNML_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HFC6392022.1"
                     /translation="MMGDSVIYYVEQADEPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESICV
                     PVDDISDWMFVCNGIPYGGFTVQAMRGQMTEEERTEHDAAWGIDFGDPGQILLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNSDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGASPASTTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            12534..12875
                     /gene="fdx"
                     /locus_tag="ACFNML_000605"
     CDS             12534..12875
                     /gene="fdx"
                     /locus_tag="ACFNML_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFC6392023.1"
                     /translation="MPKITVLPHATLCPEGAVIDNAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(13162..13749)
                     /locus_tag="ACFNML_000606"
     CDS             complement(13162..13749)
                     /locus_tag="ACFNML_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFC6392024.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLSGSGGGFT
                     VTAESVSAACPELEGRYLELVRRAAVSFGFTQGWENPAGGQEKTQAVPDAASGHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            13962..14159
                     /gene="iscX"
                     /locus_tag="ACFNML_000607"
     CDS             13962..14159
                     /gene="iscX"
                     /locus_tag="ACFNML_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFC6392025.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            complement(14227..14628)
                     /locus_tag="ACFNML_000608"
     CDS             complement(14227..14628)
                     /locus_tag="ACFNML_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HFC6392026.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            14839..15798
                     /locus_tag="ACFNML_000609"
     CDS             14839..15798
                     /locus_tag="ACFNML_000609"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HFC6392027.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQISQVSRHPQRPYTLDYIEALFTDFEELHGDRHFADDYAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPVLCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            15866..17176
                     /gene="tilS"
                     /locus_tag="ACFNML_000610"
     CDS             15866..17176
                     /gene="tilS"
                     /locus_tag="ACFNML_000610"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFC6392028.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKICVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVARGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHLLN
                     NVRALQEDLALLDEVVVQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            17227..18012
                     /locus_tag="ACFNML_000611"
     CDS             17227..18012
                     /locus_tag="ACFNML_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HFC6392029.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGMPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLARYEDRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
BASE COUNT         4171 a         4716 c         5075 g         4076 t
ORIGIN      
        1 caaacgcggc aggaatctat cggaaacggc tgaaaccgaa cggactggat tcccgcctgc
       61 gcgggaatga cggaaagtgg cgttcgggat tcccgccttc gcgggaatga cggaaagtgg
      121 cgggaataac gaaaggcggg aatgaccgcg caaaaagccg ctgccccctt cggacggcac
      181 cggcaacaaa aaaccgcacg gccgaaaccg cgcgggaaag gatagtcggg cgcgcccgat
      241 aagcagcggc cgcccccgtt atttcaattg ggcgacatat tggcgcaaaa cctcgttgat
      301 gcgcgtctgc cagcccttgc cgccggcgcg gaatttttcg accacatcgg cggacaggcg
      361 tatggtaacg agttgtttcg gggttttgcc tgtgtttcgt ttttgcatta cgcccttttc
      421 ttccaattgt ttttgatggg aaaaaagcac ctgtgccaag tcttcgggca gggcttcggc
      481 aatggggcgg gcaagcgcaa aatcttcggc ggcaagttcc cgcacttcgc cgtcagcgtt
      541 tgttaaggat tgacgttgca tattttttaa cctctctttt attcgctttg cgaaaactga
      601 tgacacggat gccgtctttt atcggcgtaa aacagacaat gtgcaggcgt tgcgtatcgc
      661 ctagataagc agcggcaaca taacgcggtt cggggtaatc aaagcagaca tcgggcacaa
      721 taacggccgt tgtccagcgt atttgcccga ctgattcaaa gggcaaattc cgctcttcga
      781 tattgcgttg atttttttcg gagtcaaatt caatcttcat tgcagcttgc agcgtatttt
      841 gtcgttacat tataagcggc aaaaaaccaa aatgtaaata caaaaaagga aaccccgaaa
      901 tgaccatcta tttcaaaaac ggcttttacg acgacacctt gggcagcatc cccgaaggcg
      961 cggttgccgt ccgcgccgaa gaatacgccg cccttttggc aggacaggcg cagggcgggc
     1021 agattgccgc agattccgac ggccgccccg ttttaacccc gccgcgcccg tccgaatacc
     1081 acgaatggga cggcaagaaa tgggaaatcg gcgaagccgc tgccgccgcc cgtttcgccg
     1141 aacaaaaaac cgccacggca ttccgcctcg cggcaaaggc ggacgaactc aaaaacagcc
     1201 tcttggcggg ctatccccaa gtggaaatcg acagctttta caggcaggaa aaagaagccc
     1261 tcgcgcggta ggcggacaac aacgccccga ccccgatgct ggcgcaaatc gccgccgcaa
     1321 ggggcgtgga attggacgtt ttgattgaaa aagttgtcga aaaatccgcc cgcctggccg
     1381 ttgccgccgg cgcgattatc ggaaagcggc agcagctcga agacaaattg aacaccatcg
     1441 aaaccgcgcc aggattggac gcgctggaaa aggaaatcga agaatggacg ctaaacatcg
     1501 gctgaaaaaa tacgtttacc acctgttggt agccatcgac caactgttca acgccctaac
     1561 cggcggcgcg gcggacgaaa ccctctcaag ccgcacctat cgcggcgcgc ggctcgccca
     1621 aaagcccaaa acccgctgga aggttttata caccctgatc aacggcgtat ttttcgaccg
     1681 ccaacactgc cggcaggcgt atatcagcga actgaaaggc aggcagcacg acgcgcggtt
     1741 caaccaaagc cgcgccgccg gggaaaaggg gacgcgatga actacttcgg catggtagag
     1801 tttctgcgcc tgatggcaat ggtgcggccg ccatttgtct tcttctccgg cacccgcagc
     1861 gaactgccta cttatcttga cctcgtggca gaactgcgcc tgaccggatg ggagcgtttt
     1921 gcaggcagcc aaaccttgac tgtgagcagc acatcaacag caattcaagc tacgacgacc
     1981 acctgattta taagttctga ccgcaagtag cgtactactt ttaaaggcat aagataatcc
     2041 ccgtttaaca gaccattaaa cggggataaa tttgtgcaaa agctaataca atttcctacg
     2101 cttcggcggt gcaaaagctg ccgccaattc gtgcaaaagc tgccgccgcc ttacattcca
     2161 gtgcaatgcc gtctgaaact tcgctaatct cgggttgccg cgcgctgtgt tgttcttcgg
     2221 tactcagcaa aaagccgtac agacgctcca atcgggcgcg gtaggcggtg aatttgttgt
     2281 agaacatccg gaaaaaagat agcgcgtttt gcagtcgcgc aaaggcttgg acggtctgct
     2341 ggatgtcgcc gattttgatt tgtccggcaa acaggcgcgg cgcttgcaaa ataatgggga
     2401 agagtttgat gccgttggtg aacatatcgt taaagccgct taagcagacg ctttgccgcg
     2461 cgatgcgcca acggttgcgg ataatggctt taaagcggtc ggaaagccgg ccgtgttcgt
     2521 gttgttcgcc gttgtaaaac gctacgcttt cggcgtggtc gcgtacgcgg atgagggaat
     2581 aacggtagtc gccgttgagt ttttcgtttt catagttgta acgaatcaag gggttgccta
     2641 tccacatcgc gataaaggtc gccaaaatca caaaaataaa gacgagccaa acaatgccgc
     2701 gcggaatgtc gaagccgaac acggtcagga tgccagccaa gccccacaaa acaacggcaa
     2761 attccaaaga ggtaacgacc gaattgacca tgccgcgtac aaattcgatg gtcgaagcga
     2821 tgaaatcctg cgcgtcctgt tggatacgtt ggtcgatgtt gtccggcgcg tggcggcgca
     2881 tttgcaggcg gtagtagttt ttgtcggcaa gccagcgcgt cgtcagcact tcattgagct
     2941 gctccgacca tttaatcgcc aagccttgat cgaggaagtc gttgacgaca ttgttaaacg
     3001 cccgtatcag caccacgccc gcgttcatcg cggcaaacat ccaaaacgcg gaggcgttca
     3061 aatcctgcat cgagtcgtaa agtcctttgg acataaaggt actcaacaca ttcagccgta
     3121 cttcggtcaa cagcagcgta accattaccg taatcagcag caagactttg accgtgcttt
     3181 tcggtgtcag gcaaagccaa agcggtgtgg aataaagctc ggtttgccat ttctgcatgg
     3241 gaaatttctt acggtatcaa tgccgtctga aaaagacggg tacagttgat tttttgatga
     3301 agtttgggga agttttgccg gtcagggtac attgcgtgtt aatttatagt ggattaaatt
     3361 taaaccagta cagcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa
     3421 gcaccaagtg agtcggttcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
     3481 atttttgtta atccactata ccatacaacc acgccggaat taagtttaaa tttgaataaa
     3541 aggttcgggt tctgcaaaat acagaacccg aaccttgttc ggatattgaa accggccgcc
     3601 cgattttggg cggtgcggct tgcaagtatc aagattcgca tatgccgtct gaagctcgga
     3661 gaggttcaga cggcatatgc ttatttgggc tgctcttcga cgaatctcgg acctttcaag
     3721 atgccgttgt gagaataggg cgacagcagg ttgtatgcgg cggttttgga aacctgataa
     3781 ccgcggtcgg tcaggctgtt ggcaatctga ttgaccactg cgctgaccaa agcccccaac
     3841 aggccgctgt tgctgttgtt gctgccttcg cggatgctgg ccgaacccga ccacaactct
     3901 tttccgttgc gggaatcgac cagccgtgct ttggcggata cggtcgtcac gctgtctaaa
     3961 atttgatatg aagtgccgta ttcggtaacc gtaatgtaca aaaccgcatc attgccgaaa
     4021 atctgatgca gtttttccgg ccggacggcg tgaatatcgg cggcattggt caagccgttt
     4081 tgtttgaagg tttcctccac gactgcggcg gggaagacgt aatagccggc ttcggaaagc
     4141 ggcgcggcgg tcgaagccag tacaccccat gttccgttga catcgggcga ttcgttcagc
     4201 ggcggaacca ccaaaattga agccggtttg ctttccttga atgacgtgta gtcgaaatcg
     4261 ggcgcttttt gaacttggca ggcagacagc gccaacacgg cggcaagccc taaaatcaaa
     4321 ggtttcatcg cttgcctcct ttacctgttt tcatcaggaa gtccataaat acgcccgatt
     4381 cgggaaacag ccttttctct tcttcaaact ggcggaacgc gccctctttg tctcccgaac
     4441 gggaaagcag cagtcccaga tgggcgtgcg cacccggggc ggcattcatt tttttgttgc
     4501 cggcttccac aaagtatttt tccatctttt cggtctgctt gcccaacgaa gtgtcgtcgt
     4561 ttttcaaacc ttcatagacg gtatcgggat agccgccgta ataatacagg gatttttgcc
     4621 cgttgccgcc gcaggcggtc agagccaaga ccgccgcaca cagcgacaaa cggctcaagg
     4681 ttttcggatt catcatttct ccttaacggt tgggttgcca tgcgccgttg tcaacagcct
     4741 gaaccaggct gttgacggct tcgcggattg ccaagtctaa aactttgccg ttcaaagtcg
     4801 catcgtagcc ggaagtgccg ccgaaaccga tgatttcacg gttggaaagt gcgtattcgc
     4861 ccgcgccctg tgcggaatag acgatttcgg aagtattgac gttgacgata ttcagagcca
     4921 cttttgcata ggcgatttgc gatttgccgc gacccaaaat gccgaagagc tgatgatcgc
     4981 cgacatctct gcgtccgaat tcggttacat cgccggtaac gacataatct gcgcctttca
     5041 ggttatgcgc tttgccggaa atgccggatt cctgttttaa tgcgttcaaa ttggtgcggt
     5101 tcagtacgtt gaagcggttg gtctgttgca ggtgcgttac cagaatggtt tttgcctggc
     5161 tgcccaaacg gtcttccccg tcggagaaaa tgcctttttg gaagctggag cggttgtcga
     5221 atgttccgac ggaaatcggg gtacgaacac cgtgatattg cgtattgtag gaggcgactt
     5281 tctctacctc gagactgcgt gaggattcgg tcgcacagcc ggtcagtgaa acggcagcgg
     5341 cggcaaggac aacggcggtg gaaacggttt tcataaaatt taccctaagg tcaagttaag
     5401 gaaataacgg gttatcatta ttgtccttat gtaaatttaa gtcaaggtgt ttgtctgtgc
     5461 gggacggatg cgcgcggaag gtacggatat ttttcaaacg gaatcgcggc gcgggcggga
     5521 tgcctgcctt ttccggattt agaggtaaac gatttcgccg ctccgccctt tgctttcggt
     5581 acttgcccac cagacaaatg cgggcagcac gtccccgtag cttttgcgtt cgcttttggc
     5641 ttcgcccgga tgggatttga tgcgttgcgg ggaattgatg gggccgggga cgaggacgtt
     5701 ggcgcgcagg ttgccgaagc gttcccattc gtcggcggcg actttgcaca ggtagttcaa
     5761 cgcggctttg gacgcgccga agccgcccca gtaggctttg ggtgtttcgc cgtggctttc
     5821 gccgacgaag atgacggacg cgtcgggcga ctgcttcagc agcgggaaca gggcgcgggt
     5881 cagccccata ggtgcgacgg tgttgatgcg gtattggttg acccattcgg cgacggtttg
     5941 gaaatccagc ggcgagaggg cgtaaaaata gccggcgcag tggacgatgc cgtccagttt
     6001 gccttgcgtg gcttcggcaa tggtggcggc gaactgattg aattcttttt cttccgcact
     6061 catcaggtcg aaacggatgg cgaagggttc ggggtgtccg gcttcgacaa tcgcgtcata
     6121 gactttttcc agttttttct gatggcgtgc caccagaatc acggttgcgc ctgccgccgc
     6181 ataggctttg gcgacctgtt cgcccagacc ttgcgatgcg ccggtaatta agatggtttt
     6241 gtcggacagt gtcgccatac tttttccttt ttggtggtcg gtcgggggta ttttagcgtt
     6301 ttgccgcacc ttgtaaagcg tcccgatacc ggccggcgtt ttcagacggc atcgctcagg
     6361 ctttgcaggc aggcttccgc cgacgcgaaa cctgattgta cggcggcttc gagcgtggcg
     6421 gggtagtctg ggtggaggta gtcgccggcg gggaagatgc ggtgccggtg caaccacgac
     6481 aaatccggcg gcggggcatc ggctgcggtt gtggcgcgtt tttcggtgat gacgcgcacg
     6541 gcttcgggtt cgcccaaatg cggaaggatg cgtttgaggt cggcgtgaac tttgtccgcc
     6601 cacgctcggt ttgtaaacgc gccgacgcgg tcggaaacgc tgatgacggc ggacacttcg
     6661 ttttcaggca gtccgagcct gccccggcaa agcaaccatt gtgccgtgcc gtcggcaagg
     6721 ccggtcagcg gggcgggcag gcggacgggt tcggcgtagc gcagatagac ggtggtgatg
     6781 gcgtgatagc gaaggttttg atatgccgtc tgaacgtgtt cgggcgtgcc ttcgggcagg
     6841 agcgcggcgg cgtggtaggg cgcggtggcg gggacggcgg catcgaaagc ttcgccgttg
     6901 acgagcactt tcccgtccgg gagggggttc agacggcata cgcgcgtttc gaggcggatg
     6961 tccgcgccga gccgttgaag ctccgccaag gcaggttcgg cgacgattgc gcccaaatcc
     7021 tgcttgggta ggagatagtc gctgccggat tttttcgtca gcacgccgtc ggacaaaacg
     7081 ttgcacaaca cgcgcaggct tgcggtttcc aaaggcgtgt tgagcgcacc ccacaccagc
     7141 ggctgccaaa actgcatcac ggcggcacgc ggcacgttcc gctgtttcag ccattgtgca
     7201 actgtcgtgt cgggctgtcc gaggcgtgcg gacttctgca aatcggacat atcggcaagc
     7261 agtttgcctt tgaatgcgga cggtacgcgc cgggcaagca gcacgccgcc caaaatatgc
     7321 agcggtgcgg gcagggggag ggcgcggaac tgtaaaccgc cgtgcatatg ccagtgcagc
     7381 ggtacgcgca aaaaggcggc acgggggtct gaaccgatgg ttttcatcag gcgcaacacg
     7441 ccccggtatg cgccgagcaa aatgtgctgc ccgttgtcca aaaaaccgaa accgtcggta
     7501 tttccggcca gtgcgcgcgc cctgccgccc gcctgccggc cggcttcaaa cagggtaacg
     7561 tcggcgtacc gcgccaaggt gacggcggcg gacagtcctg cccagcctgc gccgatgacg
     7621 gcgattttcg ggcgcggatg cggcgtgttc atcatttatt cctccaatgg ttttgcagcc
     7681 gtatctattt ccgtttctga aaatgacggt agaaaaggat acaggctaaa aataaaggca
     7741 gcagaaagcg cgcataggga tatcacggat ggtctgcatg ccagtatccg taccgtccct
     7801 gtggaacggt atcatagcgg taattgtcgg ggaaaaagtt gatccaaacc gttactgccg
     7861 gccataaggc tatatccctg actgctttca tttgcttctg ctcctgttta aattcccggc
     7921 aattcgattt caaagcgcga acgccaacgg gattgcgcgg ttacgatgca gactttcaga
     7981 cgatggttga aaccccgtcc ggcggggctg tgggaatcgg gtttgcctga cgggcggcgg
     8041 gcggtatgcg ccttatgccc gttccggcgt gccggggcgc ggtttgaatc cgaataacca
     8101 ggttttcagg gcaatgcgtt ttttgcgcgg cgaagggagg gcgattttgt atttgaggac
     8161 gttttgtgcg ccgtcccggt cgatttcgtt caatagctcg taataaaccg ccgccataac
     8221 cagtccgact ttttgggctt ttttatcggc agccggcagc agcgatacgg cttcgcagta
     8281 ggtttcacgg gcgcgtttga tttggaacgc catcaattcg gcaaaattgc ccgtcgggct
     8341 gcattgcaaa atcacgcttg cgggtacgtc aaaccgccgc atttcctcca tcggcaggta
     8401 aatccgtccc ctgcgcgcgt cttcgccgac atcgcggatg atgttggtca gttgcagcgc
     8461 aagtcccatc ttgtcggcgt attccagcgt ttggtcgtct gaaaacccca aaatccgcgc
     8521 aatcaggcag ccgaccacgc ctgcgacgcg gtggcaatac agtttcaatt cttcaaaact
     8581 gccgtaacgg gcttgaacca aatccatctg catcccgtcg attaaggctt ccagttcata
     8641 tttcggcagc ttgaaggttt ccttaacttg ccgcaaggcc tgattgacgg ggtgttccgg
     8701 catcgcgccg ccgaatacct tgtccaaatc gccgcgccac cagttcaatg tcgcctgtgc
     8761 aacatcgggg ttggaacatt cgtcaaccac atcgtccaat tcgcggcaaa aagcatataa
     8821 aaccgttacc gcatcccgtt tttcctgagt caggaaacgg aagcccgaca aaaaactgga
     8881 gcggctttct tctgcttttt ggcggcaata gtcgagtcct ttcacgattt atattcctaa
     8941 tgatgggcgg gaaaggcgga ttttatcggc atttggcggt agagggcaat ttcggcggca
     9001 cgacctaatc cttagcggtt tgctcaacta tcggcgcaaa ttctgttaaa atgccgcttt
     9061 ccttccttta cacaccgcac cgacaggcag aatttatggc tcttttgcag atttcagaac
     9121 cgggtatgtc cgccgccccg caccggcacc gtttggcggc aggcatcgat ttgggtacga
     9181 ccaacagctt ggtcgccacc gtccgcagcg gcagtgccgc ctgcctgccc gatgccgaag
     9241 ggcgcgttac cctgccttcc gtcgtccgct atctggaaaa cggcggcatt gaagtcggca
     9301 aaaccgccct gtccgcccaa aaaaccgacc cgctgaacac cgtcagctcc gccaaacgcc
     9361 ttatcgggcg gacgcttgcc gatctgcatc aaaatacgca ctacctgcct taccgtttcg
     9421 gcggcaatca acgcgttatc gaactgcata cgcggcaggg ggtgaaaacg cctgtcgaag
     9481 tgtcggcgga aatcctcaaa acccttaaat cgcgcgccga agaaaccttg ggcggcgatt
     9541 tggtcggcgt ggtgattacc gtccccgcct atttcgacga tgcccaacgc caggccacca
     9601 aagatgccgc gcgtctggcg ggtttgaacg tattgcgcct gctcaacgaa cccaccgccg
     9661 ccgcaatcgc ctacgggctg gacaacgcct cggaaggcac gtttgtcgtg tacgacttag
     9721 ggggcggcac attcgacgta tccgtattgc aactgaccaa aggactgttt gaagtcaaag
     9781 ccaccggcgg caacagcgcg ttgggcggcg acgatttcga ccaccgcctg ttctgccgcc
     9841 tgctcgaaca aaacggactc tcccaactca acgaacaaga cagccaactc ctgctctcgc
     9901 tcgtccgcgc cgccaaagaa caattaacca cgcaaaccga agcgcgcatt caggcgacgc
     9961 tttcagacgg catgccaatc gacacaagca tcagtcgcgc cgagttccac aacctgacgc
    10021 agcatttggt gatgaaaacg ctcgaaccgg tcacacaggc gttgaaagat gccggtgtcg
    10081 gtaaaaacga agtcaaaggc gtgattatgg tcggcggttc gacccgtatg ctgcacgtcc
    10141 aacaggcagt cgccaccttc ttcggacaaa ccccgctgaa caacctcaat cccgacgaag
    10201 tcgtcgcgct cggcgccgcc atacaggcaa acgtcctcgc aggcaacaaa accgacggcg
    10261 aatggctgct gctggacgtt acacctttgt cgctcggttt ggaaacctac ggcggcttgg
    10321 cggaaaaaat catcccgcgc aattccacca tccccaccgc gcgcgcgcag gactttacta
    10381 ccttcaaaga cggtcagacc gcgatgacga tacacgtcgt acaaggcgag cgcgagctcg
    10441 tttccgactg ccgcagcctt gccaaattca ccctgcgcgg cattccgcct atggcggcgg
    10501 gtgcggcgcg tatccgcgta accttccaaa tcgatgcgga cggtctgctg tccgtttccg
    10561 cccaagaaca aagcaccggc gtacaggcgc aaatcgaagt caaaccttcc tacggcttgg
    10621 acgacgacac catcacccaa atgctcaaag acagcatgag caacgccgcc gaagacatgg
    10681 cggcacgtgc ccgagccgaa gccgtggtcg aagccgaaag cctgaccgat gccgtcaacg
    10741 ccgccctcga gttggacagc gatttgctgg aagccgaaga attgcaacag attcggcaag
    10801 gcatcgccga tttgcaaggc cgtgtgaaag acggaaaagc cgaagacatc cgtgccgccg
    10861 ccgccaaact cggcagtatt accgacaatt tcgccgccaa acgcatgaac cgcaacatcc
    10921 aacgcgcgtt gactggtcaa agcgttgata atatttgacg gcaaatggtt tcagacggca
    10981 tagatataac ctgataccgt ctgaaggctc gaaaacactt gaaaaacatc gatatggaaa
    11041 agtcaggcat tgtctatctg atgaaaaccg tcatcaaggg cgtgtataaa atcggcattt
    11101 cggacgtaag caattttgaa ggcagaatgc gccatttgga aaacaacggc tatgcgaacg
    11161 ttgccggatt ggaacgcatc ctcgccgtca aaaccgacaa ttacaaagaa aaagaaaacc
    11221 tgctccatga aatttttagt aaaagcagga ccgaattgtt cgccgtggac gagaatcttg
    11281 taaaacgttt gtttttatcg cttcgcggcg aaatcgtgtt cccgaaaaac gaaacggcgg
    11341 aatcggaatt tgaaaaaagc gtccacgaac gcaggcagga agggaatgcc ggatccggtc
    11401 gcaaacaact gcttgatttg gtacggcgcg gacaccggga atacccttac gcgctgcccc
    11461 ggcttttggc gggcgcggca tcctacaagc cgaaaaaatc gaaaatccgc ctttttaaag
    11521 aagcatattt acgcaaaagc ggtacgaggc tgaccgacga aattgcagac ggcatccata
    11581 tttacacctg tttttcgcgg gcggatttgg aaaaagccta ttccgaatat ttggaacttt
    11641 tcaaatccga atcggatgcc gaaggcagaa aaccacggta aggtgcaaac agataccgta
    11701 cacgttgagg agcagtatga tgggcgattc cgtcatttat tatgtagaac aggcagacga
    11761 accggtaaac cgtgccggcg aacgcgcccg taaaacattc aaatactttt ggcgcgagct
    11821 tttttgggaa cgccgccgca tcattcccgc cttagatttt gccatggtca aagccccttt
    11881 tttccaagac ggcgaagacg gcgaaatttg cgaacatatg tggatcgacg atatttattt
    11941 tgacggtctt tacatttatg gcgtgctgaa caatgaaccc ggcgaactga ccaatgtcga
    12001 acaaggcgaa agcatttgcg ttccggttga cgacatcagc gactggatgt tcgtgtgcaa
    12061 cggcattccc tacggcggct ttaccgtaca ggcaatgcgc gggcagatga cggaagagga
    12121 gcgcaccgaa cacgatgccg catggggaat cgatttcggc gatcccgggc agatattgct
    12181 ggtgtatgaa gaaaaagaac atcccgaaaa tctggaagag catccgatgt gccggaactg
    12241 tattgacgat tttcggcaac agttgtccca aaattcggat tttctgcatg agcaggacga
    12301 agacggctat acgccgcttc atcatgaagc catcgcagga aatgcactta tggttcaagc
    12361 catgcttgaa tatggcgcaa gtcctgcctc aacgacatcg gaaggctata ccgccctcga
    12421 ttttgcccgc ctggcgggct ggcaaaatgt tgccgacctg ctcgaaccga gacattaggc
    12481 agacagtttt ccgaaaaacg gacgcaacca ctttttacag aaagacaata aaaatgccca
    12541 aaattaccgt acttccacac gcgacgttat gccccgaggg tgcagtcatc gataacgcgc
    12601 cggaaggcaa aaccgtcctc gacgtactgc tcgaccacga catcgaagtc gatcacgcct
    12661 gcgaaaaatc ctgcgcctgc accacctgcc acgtcatcat ccgcaaaggt ttcgacagtt
    12721 tggaagaacc gaccgaattg gaagaagacc tgctcgacca agcatggggt ttggaagccg
    12781 attcgcgcct gagttgtcag gcggttgtcg ccggcgagga tttgattgtg gaaatcccca
    12841 aatacaccat caaccacgcg cgcgaagaac actgaaaaca ggccgtctga agccggcacg
    12901 cttcagacgg cattgttgcg cggataaggc gcaatcgccc gaaaacaggc gttcgtacag
    12961 gcggaacttt cgattctata gcgaattaac aaaaatcagg acaaggcggc gagccgcaga
    13021 cagtacaaat agtacggcaa ggcgaggcaa cgctgtaccg gtttaaattt aattcactat
    13081 atattgattt ttatcggttt tctgacggaa cggattggtg cggcatccga ggcggattac
    13141 tcgggcgcga tgcacgggta tttatcggtt ttgcagccgg aaaaaccgcc ggcgggttac
    13201 tttcagggcg gcggtcgggc ggtatgccgg atagcgttcc actttcgctt ctatattgat
    13261 tttgattggt tttctgacgg aatgacccga tgcggcatcc gggacggctt gtgttttttc
    13321 ctgcccgcct gccggatttt cccatccttg cgtgaaaccg aaagagacgg cggcgcggcg
    13381 gacaagctcg agatagcgtc cttcaagctc cgggcaggcg gcggacacgc tttctgccgt
    13441 aaccgtgaag ccgccgcccg acccggaaag gcgttccgca atctgcgtgt agatacgcca
    13501 acgttgggcg cgcaacattg ccgcatcgca accggcagcc gctttatcca aggcaatcag
    13561 gccgcgccgg ttttggtggt gaaggcaaac cgttactctg gagaggatat gttgcccgtc
    13621 caatatttga tacagtttgc gtatcagcag aatcaggcgg tcaaactcct ccatctccga
    13681 cagggaatca ggatggtcgg aagcggtata aaccagtttg gacaattttg cggcaaacat
    13741 attgttcatc aatcttcctt gtcggttgaa accccgctct tcggggcggt agaatcagat
    13801 ttgtttggga gggacaactc ctcccagatc aggacgacac ataggctggt gcttgatgtg
    13861 ttgtccggcg agttgaaaca ttcagccatc ctcaaggtgc ggcagttttg ccgaaacata
    13921 ttctacacgg cttcaatgcc ggacgataaa aggaaattca tatgaaatgg accgacaccc
    13981 ggcgtatcgc cgaagaactc tatgacctgc acggcgaaac catcgatccc agaaccgtgc
    14041 gctttaccca actgcgcgac ctgattatgg cattgcccga atttgatgac gatcccgccc
    14101 gttgcggcga acgtattctc gaagccgtgc agcaggcatg gatagacgag gcagaataag
    14161 tttcgggaat gccgtctgaa atacggcggt acgcggttcg tgcttctgtt tgcagtggga
    14221 acggttttac cagtctcctt ttttcagcct atccagttgg cggcggtcgc gcttggtcgg
    14281 tctgccgtcg ggatgggcgg aagtgatgcg gctgaattgg tcgagctgtt tgcgctcttc
    14341 cctcaatgct gccgtttttg cgtcttcttc atacagaagc cgcgcctcgg atgccgggcg
    14401 gcgttggtgg ttcaaacctt taaccttgat tttataggga agggaattga gcgtcaggtc
    14461 gataatatcg ccgatgtcta tggttttact gtttttgacc ttcgagccgt ttacttgaac
    14521 cctacccagt tcgatgtgct tttgcgcaag ggaacgggtc ttgaaaaaac gtgccgccca
    14581 aagccatttg tccagccgca tggcggaaga atcgtgcttg tctttcatac gattttgttt
    14641 gaaataattg aatttgtttc gagtttagca taagatacgc cgcctataac tagtatatat
    14701 gcactaatcc actgttttcc atgctgtccg aacacaaaaa gagggtgtgg aaaagccgtt
    14761 ttggacaata aattaactgc ggaatatgca caaatagcgt atgatagcgg cagaatctgt
    14821 tgatgagagc ttcattctat gaaacctgtt tttttggatt ttgaacaacc catagccgaa
    14881 ctgaccaaca aaatcgatga gctgcgtttc gtccaagacg agtctgccgt cgatatttcg
    14941 gacgaaatac accgtttgca gaaaaaaagc aacgacctga ccaaatcgat tttcagcaaa
    15001 ctcacacccg cccaaatttc acaggtttcc cggcatccgc agcgtcccta tactttggat
    15061 tacattgagg cactgtttac cgattttgaa gaactgcacg gcgaccgcca ctttgccgac
    15121 gattatgcga ttgtcggcgg attggcgcgt ttcaacggac aaagcgtgat ggtcgtcggg
    15181 catcagaaag ggcgcgacac caaagaaaaa atccgccgca acttcggtat gccccgtcct
    15241 gaaggctacc gcaaagccct gcgcctgatg aagacggcag aaaaattcgg cttgcccgtc
    15301 atgaccttta tcgatacgcc gggcgcgtat cccggcatcg gcgcggaaga acgcggacag
    15361 tcggaagcca tcggcaaaaa cctgtacgaa ctgacgcgcc tgcacgtccc cgttttgtgt
    15421 accgtcatcg gcgaaggggg ctcgggcggt gcgttggcgg tcgccgtagg cgattacgtc
    15481 aatatgctgc aatattcgac ctattctgtt atctcccccg aaggctgcgc ttcgattttg
    15541 tggaaaaccg ccgaaaaggc ggcggatgcg gctcaggctt tgggcattac tgctgaccgc
    15601 ctgcaaaagc tggacttggt cgataccgtc atcaaagaac cattgggcgg cgcgcatcgg
    15661 gatttcgggc aaaccatgaa aaacgtaaaa gccgttttgg aaaaacaact gcacgaagcg
    15721 caaagcatcc cgcttgccga tttgctttcg cgccgtttcg accgcattat ggcttacggc
    15781 aaattttcgg aacaataatt caggtagaac aagcagcaag cagtttgtct gaaactgctt
    15841 gctttttctt tatcgggacg gaaccgtgct gactttagat gcgtttgagc aatgcttgaa
    15901 ggattgtttt cctcaaggtc tgaatggaaa aaaaacagcg gtggcattaa gcggcggctt
    15961 ggattccgtc gttttgctgc atctgcttgt ccgcgccggg aaaaaaggcg gttttattcc
    16021 ggatgcattg catatccatc acggcttgag tccccgtgcc gacgattggg cggatttctg
    16081 ccaaaactat tgcgatatgc tcggggtggg gctggaaacg gttaagatct gcgtggaaaa
    16141 aaacggtttg ggcatcgagg cggcggcgcg gcaaaagcgt tatgccgcgt ttgccgaaaa
    16201 aggctttgac gttttggcgt tggcgcacca cagggacgat caaatcgaaa cctttatgct
    16261 ggcggtcgcg cgcggcggcg gtttgcgcgc tttggcggct atgcccgccg tccgcccttt
    16321 tggggaaaaa ggcattatct ggcggccctt gctgcctttt tcgcgccaag atatatggga
    16381 ttatgcccaa aaacacggtt tgccgaatat cgaggacgaa agcaatagcg atacggctta
    16441 tttgcgaaac cgcttccggc accgtatttt gcccgaactt tcggcgcaga ttccccattt
    16501 cgggcggcat ctgttgaaca atgtccgtgc cttgcaggag gatttggctt tgttggacga
    16561 ggtcgtcgtt caagactgcc gttgggtttg cggagccggt tatttcgata cggcgcggtg
    16621 gctgacgttt tccccgcgcc ggaaaaccca tattttgcgg cattttttga aggaaaacgg
    16681 cattcccgtg ccgaatcaga atgcccttgc cgacattgcc cgggttttga cggaggcaaa
    16741 aaccggacgc tggaacgtgc aaggctttga attgcatcat tatgcaggca ggctgtttgt
    16801 gttccgaccg gaaaaaacgg ataaactgcg gtttttgaaa gacaggcaga taagcggaaa
    16861 tttaagggaa atattggcgg ggcagggatt tgttttaaaa cgtcatccgt tcggacttcc
    16921 cgggcatctt ttggagcagg acggaatctt gcggacggtt gcggcatcgg acaggctggc
    16981 tgtcggcggt atccataagg atgtgaagaa aatccttcag gaaaagcggg ttttgcccgc
    17041 gctgcgcccg ttttggccgg tcgtcgccga caaggacaac cgtccgctgg cgttggcaaa
    17101 ttacggtacg gattttcaaa tctcggtttc agacggcatt ttgcccgtcc atccggattt
    17161 tcccgtttta ttttgatgga atcggaatcg ccgttaccta ttatatattc ttgaaaaggc
    17221 ggtaaagtga agcatatcag ttcggcaaac aatgaacaca tcagacacct gcaccgcctg
    17281 ttgtctcaag ggaagttcag acggcaatac gcccaaaccg ttttggaggg cgtgcacctg
    17341 cttcaggttt tcctgcaatc cggcgggatg ccggtcgggg tatatattcc cgaagcgaaa
    17401 atgccgtctg aagaagtccg taaattgacg gcggttttgc cggaagacgg ggttttttcc
    17461 gtttcagacg gcatattgaa gaaaatcagc agcctgactt gcgcggatga tgtgcttgcg
    17521 ctgattgata ttccggatgc gggtgctttg ccggccggcg gcgactgtgt ggttttggac
    17581 ggcgtgcaag atccgggcaa tgtcggcacg gtgttgcgga gcgcggcggc ggcaggaatc
    17641 ggcgcggtcg ttttgggcaa aggttgtgcg gacgcgtggt cgcccaaagt gctgcgagcc
    17701 ggaatgggcg cgcatttctt gtcggagatt tatccgcagg cggatttgga aatatggttg
    17761 gcgcgttatg aagaccgtgt gtttgccacc gcgttgcgtg aggaaaaaca ggcggttctg
    17821 tataacgaag atttgcgcga accgacagcc tgggtgtttg gcaacgaagg cgcgggtgtc
    17881 ggcaaagcgg ttttagacag ggcggacaag tgcgtcagga taccgatgca cgatgcaacc
    17941 gagtctttaa atgtcgcgat ggcggcgaca atctgcctgt ttgaacagat gcggcagcgg
    18001 tcggcgtatt gaggaaaaga aatgccgtct gaaaaaat
//
LOCUS       DAXHTR010000025        17874 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.025, whole genome shotgun sequence.
ACCESSION   DAXHTR010000025 DAXHTR010000000
VERSION     DAXHTR010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17874
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.025"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            1..149
                     /locus_tag="ACFNML_000612"
                     /pseudo
     CDS             1..149
                     /locus_tag="ACFNML_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(159..1268)
                     /gene="mltB"
                     /locus_tag="ACFNML_000613"
     CDS             complement(159..1268)
                     /gene="mltB"
                     /locus_tag="ACFNML_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFC6392030.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRANEAQAPRADEMKKES
                     RPEFDAAAVFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKAD
                     IVKIMHRPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAV
                     IGIETNYGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGS
                     YAGAMGMPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILV
                     SATLAPGADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEY
                     YLGLNNFYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(1177..1428)
                     /locus_tag="ACFNML_000614"
     CDS             complement(1177..1428)
                     /locus_tag="ACFNML_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392031.1"
                     /translation="MPLDGIFGGGRQISGGAKGNARHSAKPPIAADAAFRAAPLCRFP
                     TYPICSEHIYEKEKNTAAGNLFGGFVCLYGDGGTPAPGK"
     gene            complement(1469..2896)
                     /locus_tag="ACFNML_000615"
     CDS             complement(1469..2896)
                     /locus_tag="ACFNML_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6392032.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDAAACLLNDIRKDILDCRYCG"
     gene            3081..7193
                     /gene="mfd"
                     /locus_tag="ACFNML_000616"
     CDS             3081..7193
                     /gene="mfd"
                     /locus_tag="ACFNML_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFC6392033.1"
                     /translation="MTYPIPKPREKSRWSNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTDTQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHILPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAVVSDSLKAAAVSTESSLPPGKSNLHGQIQQQPAPSP
                     VGEGWGEGKAVAAQTEFPASATNPLPSPLPQEREQSAAAVSDDLKAKSSLHLVASNLH
                     GQIRQQPTPSPVGEGWGEGKAVSAQTEFSAAAISPLPSPLPQEREQSAAAVSDDLKTK
                     SSLHPVASNLHGQIRQQPTPSPVGEGWGEGKAVAAQSAIAVITESDLYQYVARSRVHN
                     RRKKHAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLITMDLGGETNEMMLLEYAGE
                     AQLYVPVSQLHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAR
                     RAAQSGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGF
                     GKTEVALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSK
                     ATKAALEGMADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRAN
                     VDILTMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLREL
                     KRGGQVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNV
                     LLCSTIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITK
                     DAEKRLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQA
                     VRDLKKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQIN
                     AIHEELVDRFGLPEQPVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPT
                     EIILLIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            7342..7725
                     /gene="panD"
                     /locus_tag="ACFNML_000617"
     CDS             7342..7725
                     /gene="panD"
                     /locus_tag="ACFNML_000617"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFC6392034.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTISGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            7747..8589
                     /gene="kdsA"
                     /locus_tag="ACFNML_000618"
     CDS             7747..8589
                     /gene="kdsA"
                     /locus_tag="ACFNML_000618"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFC6392035.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCTHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPYQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEADNGKL
                     ILCERGSSFGYDNLVVDILGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            8604..9044
                     /locus_tag="ACFNML_000619"
     CDS             8604..9044
                     /locus_tag="ACFNML_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HFC6392036.1"
                     /translation="MMQTFRKISRYVATLWLGMQIMAGYIAAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAILTRLQTALLLFLLSALAANQFLVTPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSMIFMATALLSAVLSWRLSGKDAV"
     gene            9092..10378
                     /gene="eno"
                     /locus_tag="ACFNML_000620"
     CDS             9092..10378
                     /gene="eno"
                     /locus_tag="ACFNML_000620"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFC6392037.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            10392..10670
                     /gene="ftsB"
                     /locus_tag="ACFNML_000621"
     CDS             10392..10670
                     /gene="ftsB"
                     /locus_tag="ACFNML_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFC6392038.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(10711..11001)
                     /gene="yfaE"
                     /locus_tag="ACFNML_000622"
     CDS             complement(10711..11001)
                     /gene="yfaE"
                     /locus_tag="ACFNML_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I ribonucleotide reductase maintenance
                     protein YfaE"
                     /protein_id="HFC6392039.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCCVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(11199..12332)
                     /gene="nrdB"
                     /locus_tag="ACFNML_000623"
     CDS             complement(11199..12332)
                     /gene="nrdB"
                     /locus_tag="ACFNML_000623"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFC6392040.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGVHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(12393..13580)
                     /locus_tag="ACFNML_000624"
     CDS             complement(12393..13580)
                     /locus_tag="ACFNML_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFC6392041.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNGNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(13577..14587)
                     /gene="dcm"
                     /locus_tag="ACFNML_000625"
     CDS             complement(13577..14587)
                     /gene="dcm"
                     /locus_tag="ACFNML_000625"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HFC6392042.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KK"
     gene            complement(14645..16924)
                     /gene="nrdA"
                     /locus_tag="ACFNML_000626"
     CDS             complement(14645..16924)
                     /gene="nrdA"
                     /locus_tag="ACFNML_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFC6392043.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAADGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAAKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPDIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPRVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRAEIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            17453..17764
                     /locus_tag="ACFNML_000627"
     CDS             17453..17764
                     /locus_tag="ACFNML_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
                     /protein_id="HFC6392044.1"
                     /translation="MLAILLSAILGLVSTTAAAGTSEPAHRDTKHIRKANKQMLHPEC
                     RKYLERRAAWYRSQGNVQELRENKKARKAFHSLPYAEQKIQCRAAYEAFDDFDGGSFR
                     R"
BASE COUNT         4476 a         4769 c         4270 g         4359 t
ORIGIN      
        1 atgcaaaaat gccgtccgaa ccgaaacgtg gttcagacgg cattttatag tggattaaca
       61 aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacggcaa ggcaaggcaa
      121 cgccgtactg gtttaaattt aatccactat aaaaaacatt acaatcccgg gccgccaagc
      181 gaattggcaa tgtccctgac cgccgttaca tacatccgac tgtgattgta ctgccatacc
      241 gtataaaaat tgttcaagcc caaataatat tcaaacacgc cgggtgcggt ttccagtttg
      301 aacaaaaccg ccttttcatc atctgcaagc tcttcgccgg ggatgatgcc gtacgccttt
      361 aaatccgcca ccgtccgcgt cagggcggtt ttttcgccaa tgattgcctg aacatccgca
      421 cccggcgcca atgttgcaga caccagtatt ttcccgcccg tgcgccaacc gtgctgcttc
      481 atataattgg caaccgatgc cgcgacatcg ccgatgttgc cccatatgtc ccgatgtccg
      541 tccccgtcat aatccaccgc ccatttccgg tagctcgaag gcataaattg cggcatcccc
      601 attgcgcccg catagctgcc tttaaaggcg aaaacatcgc cgccttcttc ttttgccagc
      661 tttaaaagct cgaccaattc tttttggaaa aacccggcgc ggcgggggta atcaaagcct
      721 aaggtcgcca atgcgtccgc cacacggaaa ctgcccgtat ttttgccgta attcgtttca
      781 atcccgataa ccgccacgat aagttcggca ggcacgccgt atttttgcgc cacatcatcg
      841 ataagcgcgc ggttttccgc ataaaaccgg cgcgcgccgc gaaatttcgc cttgcccgaa
      901 tttcccgtgc ggaacacata ccacggacgc gatgtggagg ggcggtgcat aatcttgacg
      961 atgtccgcct tgtaagccgc tttgtcaaaa aaatcctgcc attccgcccg ggaaaaatcc
     1021 cctttcccga cttcatcgtc cacaaaacgg cggacatttg cattggcggc aaacccgctg
     1081 tcggataccg gtacggctgc cgcgtcaaat acggctgccg cgtcaaactc ggggcggctt
     1141 tcttttttca tttcatccgc gcggggggct tgggcttcat ttgcccgggg cgggcgtgcc
     1201 tccatcgccg tacaggcaga caaagccgcc aaacaaattg ccagcggcag tatttttctc
     1261 tttttcataa atatgttccg aacaaatagg gtaagtggga aagcggcaca agggggcggc
     1321 gcgaaatgcg gcatccgccg caatcggcgg ctttgcggaa tgccgcgcgt tgccttttgc
     1381 gccgcccgaa atctgcctgc cgccgccgaa aatgccgtct aaaggcactt cccctttcag
     1441 acggcattgc ctgcgccgta tttgcccgct atccgcaata tcggcagtcc aatatgtctt
     1501 tgcggatatc gttcagcagg caggctgccg cgtcttcgtg ttccgcctgt acgaagacaa
     1561 agagtcgggc gatgattttg ccgataaaga ctttttgcag ggcgcaggcg tcggtcagcg
     1621 tgtgttcctg caggaagctg cggatgtctt cgggtaaggt gtaatcacgg gcaacggcgg
     1681 caaagcgggc atcggtttgc aggcggtcga gcagggtttg gtttttgtcc aacttcatgc
     1741 ctgcttcttt ttcttcggcg gtggcgcgga caaactggtc gtaaatttcg gcataaagca
     1801 tatcgttcgg gccttcaaaa atcgtgaagg ggcggatgtc gatagcgata ttgccggcgg
     1861 tgtgtccgcg ttcaaaaccc ttcgcgccca agagtttttg caacatttgc gcggcggcgt
     1921 aagtgtattc cgtggcgagg gttttgacga tgttcgcctc catcagctga tgggcgacgg
     1981 gggcaacagg cgaaacggaa tggcagacgt agcggtaaag aatctcggaa acctgatggc
     2041 ggcgccggat ttcgcggcgt tcgtaatcga cgaatctgat gtcgttgcgg acgtatcgtt
     2101 ccaggttttc aaggatgtat tccataatgc cgtgcgtcat gccgatcagt tgcaggcggc
     2161 tgcggataaa gatgttttgg aacgcgcgca aaccggcagc gtcgctctgg gagagtttca
     2221 tcacggcggt tgcaggcatt tcggcatcga tgcggttgac ggcgtaacgg acggcgcgca
     2281 agccttcgga tgcgagggtt tcgcagcgga tgtatgtttt ggggacgagc agcaggtcga
     2341 tgactttggc gagtttgccg tttttgcgtt ctttggcggc aacgaggagg aagtcgcttt
     2401 gcgagttgcc ctgccagtat tttgcggcgt tgacgtaaat ggtttgtccg tcggtatatt
     2461 cgtagtaaga ctgcatttct cgggcaatcg ccgcgccgga ggtttcgggt tcggtaacgc
     2521 ccaaaccacc gccctcgcct ttgaaaacca tgtccaaacc ctgtgcgatt tgcgcttcat
     2581 cgccgaactc ttgcagtggc tgcaacacca gcgcgccttc gatgccggta cgcagcgtaa
     2641 cgggcacgcc gtaatgcccc gcaatccgca agacttcctg aatttcaaac tggctgccct
     2701 tgcgcccgcc gtgttttttg tcgaggaagg gcaacagcaa acccgcctgc ttcaaggcaa
     2761 gccatttgtc ttcgggcagg tatcgcatca ggtcgatacc gtctgaaaaa atgcggcgga
     2821 atgcggattc gatgtgcttt aaaaaagcag ccgtgtccat agttgacggc tgcgcgctcg
     2881 gttcggtgtg tatcatcggc ttcctctgtc ggttcccatt aatcggcggc cggtcaaacc
     2941 gcctgccaca gtttagagtt gattttctaa actttaccac aaagtgcgcc gggcaacaat
     3001 ccgccgacct ttcagacggc atcgcgcccc ctcccgtgct aaaatgaccg tttgcatcac
     3061 tgtccgccga ttgccgcact atgacctacc ccatccccaa accccgtgaa aaatcccgtt
     3121 ggtccaatct ttcgcaaggc tcgctgccct tggctttggc gcgttatctg ccgcataagc
     3181 ggctcaaggt cgtgctgacc caagatgcgg aacaggcgct acgccttcag acggcatggc
     3241 ggtttttccg tccgcacgac acggcggtgt tcctgccgga ctgggaaacg ctgccttacg
     3301 agcgtttttc gccgcatcag gatttggtgt cggaacggct gtcggcgttg tggcagatta
     3361 agagcggcgc ggcggacgtg ttgttcgtgc cggttgccac ggcgatgcag aagctgccgc
     3421 ccgtgccgtt tctggcaggg cgcacgtttt ggctgaaaac agggcagact ttggatatag
     3481 gccgtctgaa aaccgatttg gtagatgcgg gctacaacca tgtttcccat gttgttgcgg
     3541 cgggcgagtt tgccgtgcgc ggcggcattg tcgatttgtt ccccatgggc agcgaaatgc
     3601 cctaccgcat cgatttgttt gacgatgaaa tcgacagcat caaaaccttt gatacggaca
     3661 cgcagcgcac catctccccc gtttccgaaa tccgcctgct gccggcgcac gaattcccca
     3721 ccgacagcga ggcgcaaaaa atcttccgca gccgcttccg cgaggaagtc gacggtaatc
     3781 cgaacgatgc cgccgtgtac aaagccgtca gcaacggcca cttcggtgcg ggcgtggaat
     3841 attacctgcc gctgtttttt gaaaacgagc tggaaacgct gtttgactat atcggcgagg
     3901 atgcgctgtt tgtctctttg ggcgatgttc acgccgaggc aaaccgcttt tggagcgacg
     3961 tcaaatcgcg ttacgccatg gcgcagggcg atgaaactta tccgcctttg cttccacagc
     4021 atttgtatct ctctgccgat gtattcgcag gccgtctgaa aaactacgga caagtgctgc
     4081 ccgatgtttc cggcaaggaa cacatcctgc ccgaccttgc cgtcaaccgc caatccgacg
     4141 agccgttgca ggcattgaag gattttcaga cgacctttga cgggcggatt ttgctgtgcg
     4201 ccgaaagctt gggacggcgc gaaaccatgc tcggtttctt gcagcaaaac ggtttgaaag
     4261 ccaaacctgt gtccgactgg cagggcttct tgtcggcgca tgagccgctg atgattacag
     4321 tggcgccgtt ggcttacggg tttaaactgg gcggactgca atcgccgaac caacagcaaa
     4381 ctacttccgc ctccgaagga gaaggcgatg cggttaccga ccaaaccgaa ttttccgcat
     4441 ccgcaacaaa ccctctccct agccctctcc cacaggagag ggaacaaagt gcagccgtcg
     4501 tttcagacag tctgaaagca gccgccgttt caaccgaaag cagcctgccc cccggtaaaa
     4561 gtaatctgca cgggcaaatc caacagcaac ctgccccctc ccccgtgggg gagggttggg
     4621 gagagggcaa agcagttgcc gctcaaaccg aatttcccgc atccgcaaca aaccctctcc
     4681 ctagccctct cccacaggag agggaacaaa gtgcagccgc cgtttcagac gacctgaaag
     4741 ccaaaagcag cctgcatctc gtcgcaagta atctgcacgg gcaaatccga cagcaaccta
     4801 ctccctcccc cgtgggggag ggttggggag agggcaaagc agtttccgct caaactgaat
     4861 tttccgcagc cgcaataagc cctctcccta gccctctccc gcaggagagg gaacaaagtg
     4921 ccgccgccgt ttcagacgac ctgaaaacca aaagcagcct gcatcccgtc gcaagtaatc
     4981 tgcacgggca aatccgacag caacctactc cctcccccgt gggggagggt tggggagagg
     5041 gcaaagcagt tgccgctcaa agtgccatcg ccgtcatcac cgaatccgat ctctaccaat
     5101 acgtcgcccg ttcgcgcgtc cacaaccgcc gcaagaaaca cgccgccgtt tcagacggtc
     5161 tgttgcgcga ccttgccgaa atcaacatcg gcgaccccgt cgttcacgaa gaacacggca
     5221 tcgggcggta tatgggcttg atcacaatgg atttgggcgg cgaaaccaac gaaatgatgt
     5281 tgcttgaata tgcaggcgaa gcgcagcttt atgtgcctgt ttcgcaactg catttaatca
     5341 gccgctactc cggtcaggcg catgaaaacg tcgccctgca caagctcggc agcggcgcgt
     5401 ggaacaaagc gaaacgcaaa gccgccgaaa aagcgcgcga taccgccgcc gagttgctta
     5461 acctttacgc ccgacgcgct gcccaatcgg gacacaagtt tgaaatcaac gagttggatt
     5521 atcaggcgtt tgccgacggc ttcggctacg aagaaaccga agaccaggca gccgccatcg
     5581 ccgccgtgat taaagatttg acgcaggcga agccgatgga ccgcctcgtg tgcggcgatg
     5641 tcggtttcgg caaaaccgaa gtcgccctgc gcgccgcgtt tgtggcggta atgggtggca
     5701 aacaagttgc cgtactcgca ccgaccacgc ttctggtcga gcagcacgcg caaaattttg
     5761 ccgaccgctt cgccgatttc cccgtgaaag tcgccagcct ttcgcgtttc aacaacagca
     5821 aagccaccaa agccgcgctg gaaggcatgg cggacggcac ggtcgacatc gttatcggca
     5881 cgcacaaact ggtgcaggac gacatcaaat tcaaaaactt aggtttagtg attatcgacg
     5941 aagaacaccg cttcggtgtg cgccaaaaag agcagctcaa acgcctgcgt gccaatgtcg
     6001 acatcctcac catgaccgcc acgccgattc cgcgtacttt aagtatggcg ttggaaggac
     6061 tgcgcgactt ctcgctgatt accaccgcgc ccagccgccg cctcgccgtc aaaacctttg
     6121 tcaaaccctt tagcgaaggc agcgtgcgcg aagccgtgtt gcgcgaactc aaacgcggag
     6181 gacaggtatt tttcctgcac aatgaagtag atacgattga aaatatgcgc gagcggctgg
     6241 aaaccctgct gcccgaagcc cgcatcggcg tggcgcacgg acaactgcgc gagcgcgagc
     6301 tggaacaagt catgcgcgac tttttacagc aacgattcaa cgtgttgctc tgttccacca
     6361 tcatcgaaac cggtatcgac attcccaacg ccaacaccat catcatcaac cgcgccgaca
     6421 aattcggact ggcgcaactg caccagcttc gcgggcgcgt cggacgcagc caccaccaag
     6481 cctacgccta cctgctcacg cccgaataca tcaccaaaga cgcagaaaaa cgcctcgacg
     6541 ccatcgcggc agcagacgaa ctcggcgcag gtttcaccct cgccatgcag gatttggaaa
     6601 tccgcggcgc aggcgaaatc ctcggcgaag ggcagtccgg cgaaatgata caggtcggct
     6661 tcacgctcta caccgaaatg ctcaaacaag ccgtgcgcga tctcaaaaaa ggccgccaac
     6721 ccgacctcga cgcgccgctg ggcatcacca ccgaaatcaa actgcacagc cccgccctac
     6781 tgcccgaaag ctactgcccc gacatccacg agcgtctcgt cctctacaaa cgcctcgccg
     6841 tctgcgaaac cgtgcagcaa atcaacgcca tacacgaaga actcgtcgac cgcttcggcc
     6901 tgcccgaaca acccgtcaaa acccttatcg aaagccacca cctgcggctt atggcaaaag
     6961 aattgggtat cgatgccatt gatgcggccg gcgaagcggt aacggtaacc ttcggtaaaa
     7021 acaataatgt cgatccaacc gaaatcatcc tgctgattca gaacgacaaa aaataccgcc
     7081 ttgccggcgc cgataagctg cggtttaccg cagagatgga aaatatcgag gtcagaatca
     7141 acaccgtaaa aaacgtttta aaaaccttgc aaaacagatg cctgcccaaa taaagccgac
     7201 accgcaatgc cgtctgaaac accgttttcc ttgtccgaaa gccgccatta tgaatttgaa
     7261 ggaaactcca ctataatacg gcattcagat ttccagacgg catcgcgccc gtcagaccgc
     7321 acacaaacca aaaggaaata catgttccgt actatgcttg gcggaaaaat ccaccgcgcc
     7381 accgtaaccg aagccgattt gaactacgtc ggcagcatta ccgtcgatca agatctgtta
     7441 gacgcggcag gcatctaccc caacgaaaaa gtcgccatcg tcaacaacaa caacggcgaa
     7501 cgttttgaaa cctataccat ttcaggtaaa cgcggcagcg gcgtgatttg tctgaacggt
     7561 gctgcggcaa ggctggtgca gaaaggcgat atcgtcatca tcatgtctta cgtccaactc
     7621 tccgaacccg aaatcgccgc gcatgaaccc aaagtcgtct tggtagacgg aaacaacaaa
     7681 atccgcgaca tcatctccta cgagccgccg cacaccgtgc tgtaaccctg aaaacggaca
     7741 ttgatcatgg atattaaaat taacggcatt accgtcggta acgatgctcc tttcgtactg
     7801 ttcggcggca tcaacgtttt agaagatttg gattccaccc tccaaacctg tacgcattac
     7861 gtcgaagtta cccgaaaact cggtattccc tatatcttta aagcatcttt cgacaaggca
     7921 aaccgttcct ccatccattc ttatcgcggc gtaggcttgg aagaaggctt aaagattttt
     7981 gaaaaagtca aagcggaatt cggcatcccc gtcattaccg acgtacacga accctatcag
     8041 tgccaacccg tcgccgaagt gtgcgatgtc atccagcttc ccgcattcct tgcgcggcag
     8101 accgatttag tggttgccat ggcaaaaacc ggcaacgtcg tcaacatcaa aaaacctcag
     8161 ttcctcagcc cctctcaaat gaaaaacatt gtggaaaaat tccacgaagc cgacaacggg
     8221 aaactgattt tatgcgaacg cggcagcagc ttcggctacg acaacctcgt tgtcgatata
     8281 ctcggtttcg gcgtgatgaa acagacttgc ggcaacctgc cggttatttt cgacgttacc
     8341 cattccctgc aaacccgcga tgccggttct gccgcatccg gcggtcgtcg cgcacaggct
     8401 ttggatttgg cacttgcagg catggcaacc cgccttgccg gtctgttcct cgaatcgcac
     8461 cccgatccga aactggcaaa atgcgacggc cccagcgcgc tgccgctgca ccttttagaa
     8521 gattttttaa tccgcatcaa agcattggac gatttaatca aatcacaacc gattttaaca
     8581 atcgagtaac acggtttcgc cttatgatgc agactttccg aaaaatcagc cggtatgtcg
     8641 caaccttgtg gctcggtatg cagattatgg cgggttatat cgccgcaccg gtgctgttca
     8701 aaatgctgcc caaaatgcag gcgggcgaaa ttgccggcgt attgttcgac atcctctctt
     8761 ggagcgggct tgccgtttgg ggcgcggtgt tggctgccgc ctttgccatc ctgacccgac
     8821 tgcaaaccgc cctgctgctt tttttattgt ccgcccttgc cgccaaccaa tttttggtta
     8881 cccccgttat cgaggcactg aaatacgggc atgaaaattg gctgttgtcg tttgtaggcg
     8941 gatccttcgg aatgtggcac ggtatttcca gcatgatttt catggcaacc gccctacttt
     9001 cagcagtttt aagttggcgg ctttccggca aagatgccgt ctgaagccct cccatttttt
     9061 tacctccctt cacttcactt ggagaacatt catgagcgca atcgttgata ttttcgcccg
     9121 cgaaattttg gactcacgcg gcaaccccac agtcgagtgt gatgtattgc tcgaatccgg
     9181 cgtaatggga cgcgcagccg taccgagcgg cgcgtccacc ggtcaaaaag aggctttgga
     9241 acttcgcgac ggcgacaaat cccgttattc gggcaagggc gtattgaagg cggtcgaaca
     9301 cgtcaacaac caaatcgccc aagccctcat tggtatcgat gccaacgagc aatcttatat
     9361 cgaccaaatc atgatcgaat tggacggtac tgaaaacaaa ggcaatttgg gtgcgaatgc
     9421 gactttggcg gtttctatgg cggttgcacg cgccgctgcc gaagactcag gcctgccgct
     9481 ttaccgctac ttgggcggcg caggcccgat gtccctgccc gtaccgatga tgaacgtcat
     9541 caacggcggc gaacacgcca acaacagcct gaacatccaa gagtttatga ttatgcccgt
     9601 cggcgcaaaa tctttccgcg aagcgttgcg ctgcggtgcg gaaattttcc acgccttgaa
     9661 aaaactgtgc gacagcaaag gcttcccgac cacagtcggc gacgaaggcg gtttcgcccc
     9721 caacctgaac agccacaaag aagccctgca actgatggtc gaggcgaccg aagccgccgg
     9781 ctacaaagcg ggcgaagacg tattattcgc attggactgc gcctccagcg agttctacaa
     9841 agacggcaaa taccacttgg aagccgaagg ccgctcctac accaacgcgg aatttgccga
     9901 atatctggaa agtctggtca acgagttccc catcatctcc atcgaagacg gcatggatga
     9961 aaacgactgg gaaggctgga aactgctgac cgaaaaactg ggcggcaaag tccaactcgt
    10021 tggcgacgac ctcttcgtta ccaacccgaa aatccttgcc gaaggcattg aaaaaggcgt
    10081 ggcaaacgca ctattggtca aagtcaacca aatcggtact ttgagtgaaa ccctgaaagc
    10141 cgtcgatctg gcaaaacgca accgctacgc cagcgtgatg agccaccgct ccggcgaaac
    10201 cgaagacagc accattgccg acttggcagt cgccaccaac tgtatgcaga tcaaaaccgg
    10261 ttctttgagc cgttccgacc gcatggcgaa atacaaccaa ctgctgcgta tcgaggaaga
    10321 attggcggaa gccgccgact accccggcaa agccgcattc taccaactgg gcaaataaaa
    10381 aaggttaagg tatgaagtgg gtaactgtcg ttttatcctt cgcacttgtc tgttgccaat
    10441 acagcctctg gttcggcaaa ggcagcatcg gacgcaacag cagtctgaga gaacagattg
    10501 ccgttcaaga agaaaaaaac cagacactcg ccctacgcaa tcattccctt gccgccgaag
    10561 tctatgattt ggaaaacggt caagaagcca tttcggaaat cgcccgggta gaactgggtt
    10621 atatccaaga cggtgaaacc ttttaccgac tcatcaggca taaccggtaa taccgtcaaa
    10681 aagccgtccg aaccaatgtt cggacggctt ttatttcaac aaactgtcag acagcccctc
    10741 atcctccccc gacaaaccgc aatccagcct gacatccccc tcgacgcaac agcagcacgg
    10801 cagtatctcg tcccgcccca aaaaagccaa aggcggctcc cgataagtaa cgcttccctc
    10861 caaaatcttc actcggcacg atccgcaata tccgcttcgg cactgatatt ccaccatatg
    10921 ccccgtccgt tccaagcctt ccaacagggt ctcgccctcc aagagctcaa aaaaaccctt
    10981 attcgtacca atgcgcgcca tttccgacca atcaaaaata tagtggatta aatttaaacc
    11041 agtacggcgt tgcctcgcct tgtccggatt tttgttaatc cactataatc cactataaaa
    11101 ggaacaataa ccgatcctac ccgctgtttt tcccatcata caacatacaa atgccgtctg
    11161 aaacatccgg cttcagacgg cattttttca aaaaacattt acaactcaaa atcgcccaaa
    11221 tcatccgtat tcacttcaga atctatctga ccgatcaaat aagaggatat ttccacttcc
    11281 tgcggcgcga cctgtacgtt gtcggacgac agccacgcat taatccacgg aatcgggttt
    11341 tgatttgcgc cttcaaatcc ggccggcaac cccaccgcct gcatacgcag attggtaata
    11401 tattcgacgt attgggataa gatttctttg ttcaaaccga tcatcgaacc gtccttaaac
    11461 aaatatgccg cccattcttt ttcctgttcc gccgcttttt tgaagagttg gaaacattcg
    11521 tcctgcaact cggcggcaat ttccgccatt tcagaatcat caacaccaga acgcatcaga
    11581 ttaagcatat gctgcgtgcc ggtcaggtgc agggcttcgt cgcgggcaat cagtttgatg
    11641 attttggcgt tgccttccat caactcgcgc tcggcaaaag caaacgagca ggcgaatgaa
    11701 acgtagaaac ggatggcttc caacacattg acgcacatca ggcagagata gagttttttc
    11761 ttcaacccgc gcaaagacac ggtaacgggt ttgccgccga cattgtgcac cccttcgccc
    11821 aacaggttgt aatactgggt gtattcgatt aagtcatcgt aatagcaggc aatgtcttcg
    11881 gcgcgggcgg taatgtattc gttttcgaca atatcatcaa acacgaccga cggatcattc
    11941 acaatattgc ggatgatgtg ggtatagctg cgcgagtgga tggtttcgct gaagctccac
    12001 gtttcaatcc acgtttccaa ctcgggaatc gaaaccaaag gcagcaaagc aacattcgga
    12061 ctgcgccctt ggatggaatc gagcagtgtt tggtatttca gattgctgat gaaaatatgt
    12121 ttttcgtgtt ctggcaggtt ggcgtagtcg atacggtcgc gcgacacgtc gatttcttcc
    12181 ggccgccaaa aaaacgacaa ttgtttttca atcagttttt caaatacctc gtatttctgc
    12241 tggtcataac gggcgacatt aaccggctga ccgaaaaaca tcggctcatt cagcgcgtcg
    12301 tttttggttt tgggaaaggt gctgtatgac atagttaagt gttcgcagga cataatctat
    12361 tctctatatt aattaaaaat gatctgtaag ttttaactaa cctgctgaga ctttatatat
    12421 ttaataaaat tttctaaagt taaaacatgg gttttcccaa aagttccaaa tgtttttacg
    12481 gattctttag ggacaactaa ggttaaattc tcatcactca tttcctgaat ttgagtatca
    12541 gatatacctt cttgtagagt aaataaatgc ttatgaggaa ttcgatctgc ttcatttaaa
    12601 acttggcgcc agcgatcctt acaagtagtc tttgaaccta gcattgtcag cttttcagtt
    12661 tgaaatatga aattccctga ttcatccatc gcatggtact cttcacttcc aggaaataaa
    12721 aaatctggtt tctttttccc ttctgtcttc gcttgcgtct caaattttaa ggaaaattcg
    12781 tcaaatattc ttgataaatg taactctaaa gattttcctg ctcttgcttt tctgcgattt
    12841 gtaaaagaat gtgcaaaatt aatgaagcta tccaaatcag taatttttga tttaataatt
    12901 tcaaattcac gtttttcaaa aatggaaaat aattcatatt cctttgaaac ccattgtttt
    12961 aacacattac taacgttagt tttatttaat gcaatatttt cccttgccaa aactgccatt
    13021 tcttcagttt tggggaagtt aggaaatagt gaaaataatt ttgttaggtt atcctctact
    13081 tcttgttttt taggtaaata taaacttcct ggtaaaatgt tagtttctgc gataaatatt
    13141 tcaatatctt catctgaaga taaaacaaaa gcatggaata ataaatcttt ataacaagct
    13201 ctgcacaata ccaataagga accactatat ttatcactta aaaattcaaa attcttgcca
    13261 aaacccgtaa ttctcgcctc atttctcgtg ccttggccgt aatatttaaa attgccatta
    13321 gtgacgcttc catcttgcca attaattttg attgaaatag ttttatttgt tcccttttgg
    13381 caaactacat caaagaggtc gtctgaaaaa tgtttttgaa tataaaatcc tgattggtga
    13441 ctaccagtag taccaacatc attgggacga atataacgac agtatactgc aattgattta
    13501 tttgctgtct gtattgctga agctactaag tcatcggatt tatttactac ttgtattgtc
    13561 tgagctgcca agtcattcat ttttttaact catttataat ttgtttagca atagcttgaa
    13621 ccaacggtac agcaattgaa ttgccaaact gcttgtatgc agctgtcttg gatactgcat
    13681 caataacaaa atctttagga aatcccatta aacgcgagca ctccctaggt gtcagcttcc
    13741 taggattttt tcctttctga gggatgagta tttcggaacc atctttgtaa tatcgtgcag
    13801 atagagttcg tgatattcca tctaaatcaa ctaatccaaa accaaatcca ttaccctttg
    13861 ccttatgttt tttagcgtaa ttttgaaggt aaagccataa gttatcagaa agagtaaaag
    13921 aattatctac atcatcttcc aaaatttgct ttaattttgg ttgtgattct ggtggggaag
    13981 gaaaattgaa atttatttcc ttattaaaat attgtctatc aaaacctaca ataaaaatac
    14041 gctccctatt ttgaggaaca taatattttg cattcataac ttgataaaat atctgatagt
    14101 caagctcttc taaagtccct ttaattactt taaatgtatt tcctttgtca tgcgaaacaa
    14161 ggtttttcac attctctaaa agaaaaattt taggtcgatg ttttccaata atttcagcaa
    14221 catcaaaaaa tagagttccc tgcgccttat ctaagaagcc tgtttctcgt cctaggcttt
    14281 ttttctttga aacaccagct atagagaatg gctgacacgg gaatcctgct gttaatacat
    14341 caaacttact tggaatagct gctttggttt cctttaatgt aatatctcca taaggaatat
    14401 cattaaaatt tacttggtag gtttgacggg ctttatcatc ccattcacta gaaaatacac
    14461 atcgcccacc aacattctcc attgcaatgc gaaaaccacc tattcccgca aataaatcaa
    14521 taaaagtaaa tttctcatta tttaaggtaa ctatattatc taattgattt tgtattttat
    14581 ttttcatatt ttatatttca gatgatttat taatctaaag gccatctgaa aactccccct
    14641 ttcatcaaat cttacaagcc ccgcccgcgc agccgtcatc ctgaatatcg gtctgcgtat
    14701 cgtccgcacc gtcgcgggtg ttatggtagt acagggtttt gacgccgtat ttgtaggcgg
    14761 tcagcaggtc tttgagcatt tgtttcatag aaactttgcc gccttcgaat ttgcccgggt
    14821 cgtaggcggt attggcggaa atcgattgat cgacgaattt ttgcatcacg ccgacaagtt
    14881 tcaggtagcc ttcgttgccg ggaagctgcc acagggtttc ataggcattt ttcagggttt
    14941 caaactccgg cacgacttgt ttcaaaatgc cgtctttcga tgctttgacc gttaccaatc
    15001 cgcgcggcgg ctcgatgccg ttggtggcgt tggcgatttg agagctggtt tcagacggca
    15061 tgagcgcggt cagagtagag ttgcgcaggc cgtatttgac gatttcggca cgcaggcttt
    15121 cccagtcgta atgcaaaggc tcgccgcaga cggcatccaa atcttttttg taggtgtcga
    15181 tgggcagttt gccttgcgaa taaacggttt ggttaaagag cgtacacgca ccgtattctt
    15241 tggcaaggtt tgccgatgct ttgagcaggt aatactgtat ggcttcaaag gtacggtggg
    15301 tcagaccgag cgcggagccg tcgctgtagc ggacaccgtt tttcgccaga taataagcat
    15361 agttaatcac gccgataccg agcgaacggc ggcccatagt ggaggtacgc gcggcttcta
    15421 ccggatatcc ctgataatct aaaagtgcat cgagtgcgcg cacagtcaaa tcggcaagcc
    15481 cttccaattc gtccaggctg tttaatgcgc ccaagttgaa ggcggacagg gtacacaggg
    15541 cgatttcgcc gttcggatcg ttgatattgt ccagcggttt ggtcggcagg gcgatttcca
    15601 tacacaagtt ggactgatga acaggcgcga cgcgcggatc gaacgggctg tgcgtattgc
    15661 agtgatcgac gttttgaatg tagatgcgcc cggttccggc acgctcctgc atcagcgtgg
    15721 aaaacaggtc ggcagccgga atgatgcgct tgcggatatc agggtcttgc tcgtatttcg
    15781 tatagagccg ctcaaattcg tcttggtcgg caaaaaacgc ttcgtacaat cccggaacct
    15841 cgttgggcga aaacagcgta atgttgccgc ccttaatcag gcgggtgtac agtaggcggt
    15901 tgatttgcac accgtaatca agctggcgga tacggttgtc ttccacgccc cggttgtttt
    15961 tcaacaccag caggctttcg gcttcgatat gccacaaggg gtagaacaag gttgccgcgc
    16021 cgccgcgcac gccgccttgc gaacaggatt tgaccgccgc ctgaaacatt ttgaaaaacg
    16081 ggatgcagcc ggtatgccgc gcttcgccgc cccggatttc gctgtccaaa ccgcggatac
    16141 gtccggcatt gatgccgatg cccgcacgct gggaaacgta tttcacaatc gcgctggtag
    16201 tggcattgat ggaatccaaa ctatcgtcgc attcaatcag cacacagctt gagaactggc
    16261 gcgtaggcgt acgcacgccg ctcataatcg gggtcggcag cgatacttta aatgtggaaa
    16321 cggcatcgta aaaccgtttg acgtaatcga ggcgtgtttc tttgggatat ttgctgaaaa
    16381 gacacatcgc caccagaaca tataagaact gcggtgtttc ataaatctgg cgggtaacgc
    16441 ggttctgtac cagatatttg ccttcgagct gtttgacagc ggcataggaa aaggacatat
    16501 cgcgttcgtg gtcgatatag gcgttcagct cgtcaaattc ttcgcggctg taatcctcaa
    16561 ggatatgcct gtcgtatttt gcggcatcgg taagtttttt aacgtggtcg taaaggtgcg
    16621 gcggctcgta ctcgccgtag gctattttac gcagatggaa aatcgccaaa cgcgcggcaa
    16681 ggtattggta gtccggggta tcttccgaaa ttaaatcggc agcggctttg atgatggttt
    16741 cgtggatgtc gtcggtgcgg atgccgttgt agaactggat gtgcgatttc aactcgacct
    16801 gagatacgga aacattttcc aatccgtccg ccgcccaagt gacgacacgg tgaatcttat
    16861 ccaaatcaat ggcttctaat cttccgtctc gtttggttac tttcaaatca gtcggtgtat
    16921 tcatcgcttc ctcttccact cttgatattc aagacacggt cttttcaaat aaattaaggc
    16981 agacaatata gtggattttt ggcattttct ccagtcttga caacggttgt atttttcagt
    17041 ttcaggcaat gcgcgataaa acccgcctgt cgtatttttc ctataatatt tgttttatct
    17101 gataattctt taccgataaa aaacgggtaa attttttgcc ttttgaccgg ctccggctac
    17161 aaggcggtga aataaggatt ttccgacgaa aaaggaaagc ttcctgtttt ctgcccctgc
    17221 aaattgttaa attttgcaaa gtatgatttt gccgcgccgc cgccgacaaa ttccattttc
    17281 ttaccgattg gaatttatta ttgagattaa tgtgttattt gaatctgcat atcaaacggc
    17341 aagttttgtc ggctgaatga gtctgaaact gccgacaatt tgcccgctcc atccttccat
    17401 cctatactgg aaggaatgac aaactgaaag gatcgtcatg tctgtcaaaa agatgcttgc
    17461 catactgttg tctgcaatat tgggactggt atcgacaact gccgctgccg gtacgtcaga
    17521 acccgcccac cgcgatacca aacatatccg caaggcaaac aagcagatgc tgcaccccga
    17581 atgcaggaaa tatttggaac gccgtgccgc gtggtaccga tcgcaaggca acgtgcagga
    17641 attgcgcgaa aacaaaaagg cgcgcaaagc attccactcc ctgccttatg cggaacagaa
    17701 aatccaatgc cgggcggctt atgaggcttt cgatgatttc gacggcggca gtttccgccg
    17761 ttaatcccat ataaaatatg ccgtctgaac acaggttcag acggcatttt ccatagatac
    17821 aacaaactat ccttacgcca tttcgtttga ggtcctcccg ttgccgcagc ctta
//
LOCUS       DAXHTR010000026        17506 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.026, whole genome shotgun sequence.
ACCESSION   DAXHTR010000026 DAXHTR010000000
VERSION     DAXHTR010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.026"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..1048)
                     /locus_tag="ACFNML_000628"
     CDS             complement(<1..1048)
                     /locus_tag="ACFNML_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HFC6392045.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIS"
     gene            complement(1049..1735)
                     /gene="neuA"
                     /locus_tag="ACFNML_000629"
     CDS             complement(1049..1735)
                     /gene="neuA"
                     /locus_tag="ACFNML_000629"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HFC6392046.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            complement(1739..2872)
                     /gene="neuC"
                     /locus_tag="ACFNML_000630"
     CDS             complement(1739..2872)
                     /gene="neuC"
                     /locus_tag="ACFNML_000630"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0006047 - UDP-N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase"
                     /protein_id="HFC6392047.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTYKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGAAVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKYQSDKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            3015..4190
                     /gene="ctrA"
                     /locus_tag="ACFNML_000631"
     CDS             3015..4190
                     /gene="ctrA"
                     /locus_tag="ACFNML_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HFC6392048.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            4205..5368
                     /gene="ctrB"
                     /locus_tag="ACFNML_000632"
     CDS             4205..5368
                     /gene="ctrB"
                     /locus_tag="ACFNML_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HFC6392049.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDAFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            5368..6165
                     /locus_tag="ACFNML_000633"
     CDS             5368..6165
                     /locus_tag="ACFNML_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6392050.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLRADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGSDVITYENPWYIVLCNLVLLLFGLAMVSKFSKGVEPQ"
     gene            6162..6812
                     /locus_tag="ACFNML_000634"
     CDS             6162..6812
                     /locus_tag="ACFNML_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6392051.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMHQFEDMDKAYEYYNSLP"
     gene            6877..9150
                     /locus_tag="ACFNML_000635"
     CDS             6877..9150
                     /locus_tag="ACFNML_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFC6392052.1"
                     /translation="MNITQILSQELSATTAQINAAIELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAAAQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIACRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALGRLKEAADTDAITVFARNLKDLLLVAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGKEPLDASAVHPEAYPVVA
                     KMLAQQGISAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            9181..10011
                     /locus_tag="ACFNML_000636"
     CDS             9181..10011
                     /locus_tag="ACFNML_000636"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6392053.1"
                     /translation="MHTPLTYIDLFSGAGGLSLGFEQAGFQQLLSVEMESDYCQTYRT
                     NFPHHQLLQKDLTTLTEQDLINCLNGQAVDLIIGGPPCQGFSMAGKIGRTFTDDPRNH
                     LFKEFVRIVKIVQPYFFVMENVARLYTHNSGKTRIEIIQAFQNIGYSVECKILSAADF
                     GVPQIRSRVIFIGRRDKGKISFPEPLQISHQTVGSAIGHFPKLAAGESNPHVANHEAM
                     NHSAQMLEKMAFVKNGGNRNDIPEPLRPKTGDIRKYIRYNSNKTSRLYYRRYAQSFSL
                     "
     gene            9989..10183
                     /locus_tag="ACFNML_000637"
     CDS             9989..10183
                     /locus_tag="ACFNML_000637"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6392054.1"
                     /translation="MRKVFHYEQNRALTVRELAALQSFPDNFIFCGSKIAQQQQVGNA
                     VPPLLAKAIAESILKMSENE"
     gene            10176..10520
                     /locus_tag="ACFNML_000638"
     CDS             10176..10520
                     /locus_tag="ACFNML_000638"
                     /inference="COORDINATES: protein motif:HMM:NF014177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC6392055.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDGAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            10599..11219
                     /gene="galE"
                     /locus_tag="ACFNML_000639"
                     /pseudo
     CDS             10599..11219
                     /gene="galE"
                     /locus_tag="ACFNML_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
     gene            11220..12260
                     /gene="rfbB"
                     /locus_tag="ACFNML_000640"
     CDS             11220..12260
                     /gene="rfbB"
                     /locus_tag="ACFNML_000640"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6392056.1"
                     /translation="MQTANKKTILVTGGAGFIGSAVVRHIIQNTQDSVVNLDKLTYAG
                     NLESLTDIADNPRYAFEQVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIDSAGEF
                     IQTNIVGTFNLLEAARAYRQQMPSEKHEAFRFHHISTDEVYGDLSGTDDLFTETAPYA
                     PSSPYSASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKP
                     LPVYGDGMQIRDWLFVEDHARALYQVVTEGVVGETYNIGGHNEKANIEVVKTICALLE
                     ELAPEKPAGVARYEDLITFVQDRPGHDARYAVDTAKIRRDLGWQPLETFESGLRKTVQ
                     WYLDNKTRRQNA"
     gene            12366..13232
                     /gene="rfbA"
                     /locus_tag="ACFNML_000641"
     CDS             12366..13232
                     /gene="rfbA"
                     /locus_tag="ACFNML_000641"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6392057.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNRMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            13286..13843
                     /gene="rfbC"
                     /locus_tag="ACFNML_000642"
     CDS             13286..13843
                     /gene="rfbC"
                     /locus_tag="ACFNML_000642"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6392058.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLIWNDPTVGIGWPLQTA
                     PLLSPKDLAGKTWAQAEKLRLPLSR"
     gene            complement(13886..15904)
                     /locus_tag="ACFNML_000643"
     CDS             complement(13886..15904)
                     /locus_tag="ACFNML_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HFC6392059.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDMEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPAAERAEQDLSPKAMIFWVLAMMFVLGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSV
                     VVISLVLLLVGESGGLLADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDTAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIVVDLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNAERIGTLFSAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMFFFAQRVLKAGK"
     gene            complement(16100..17221)
                     /gene="dnaJ"
                     /locus_tag="ACFNML_000644"
     CDS             complement(16100..17221)
                     /gene="dnaJ"
                     /locus_tag="ACFNML_000644"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFC6392060.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
BASE COUNT         4591 a         4255 c         4242 g         4418 t
ORIGIN      
        1 aactaatatc agtttttttg atttgagcac ctttgcgaat attgcaagca gcgaccttac
       61 caaataatgt ttcatattcg ttgacgctga agtctccatt gcctgggcgt ttaacccata
      121 ggttatctcc ggacaacagt tctccttttt taatgtcttt atctgctacg acagatgcaa
      181 aggcgaaatc tttagttggc ttttctcccg cgataatcgt gtcttttttg ccgccgcgtg
      241 ccaattttaa agcatgagcg ccttgcttga gctctttaaa agtatccgga ttcatagagc
      301 atacaatatc cggacctggg cgatccatgc ggtcagtaaa gtgacgctct aaaatcgaac
      361 cgcctaaagc tactgctcct aagcaagcat agttatctaa ggtatggtca gacaggccaa
      421 tgattgcgtc tggaaaggct tcagataaat cgttcatacc acccaatcga acatcttcgt
      481 aaggggttgg gtagatgttg gtacagtgaa gcaaagcata aggtacccct gcttctcgaa
      541 taatttctac cgactttttg atgctttcaa tagaattcat gccggtagag agaataatag
      601 gcttaccaaa agaggccacc agtttaatta atgggtagtt attacattcg ccagagccga
      661 ttttatatgc tggaatatcc atacgttgta atcgtaaagc agctgcacga gagaaaggag
      721 tactgataaa aatcataccc ttactctcta cgtattcttt taatttaatc tcatcttctt
      781 cattcagggc gcaacgttcc ataatttcat aaatagagac atctgcattg cctggaatga
      841 cttgtttggc ctcatcagac atttcgtctt caacgatgtg tgtttgatgt ttaacaactt
      901 cagcgcctgc attataggca gcatcaacca tttcaaaagc tgtttttaaa gagccttcat
      961 gattgatgcc gatttcacag ataatcaatg gttcgtggtt gtaacctact gaacgattac
     1021 caattttaaa ttcgttgttg ttttgcattt agctttcctt gtgattaaga atgttttctg
     1081 cctgttgtaa atcaagctca gtatcaatat cgatagagtc ttgatgagac ataatataaa
     1141 gtttggttgg ggcgataaaa aaacaattat ttgcaattag tgaagcagta tcattaatgt
     1201 aaattgcacc attaggccta aatgcctgag gtaattgttg gcgaggctgc tccaaatcgc
     1261 ttagatggcg catgggggca tattcgccat tattgatttg aagcagggtt tttagtggat
     1321 gatgctccat tgggcatgca gagacaacgg atccttttat tttctcatca aatagagaaa
     1381 aagcttcacg aatatgagcc cctgtgcgta atggactggt tggttgtaat agggttactg
     1441 tgccggaatt actgccaatt gtttctaaag catgtattac acctgaaata gagctggctg
     1501 tatcggaggc cagctctgca gggcgtagga cgacttcgac accgaaattt ttagcttctt
     1561 ctgcaattaa cccgccatca gtcgaaacaa ttatgcggtc aaaacacttt gatgatatag
     1621 cagcattaat tgtatgacca agtaatgata tgccattcat tttccggaga ttttttaatg
     1681 gcaatccttt ggagttttgg cgcgcaagta taaccgcaat attttgtttt tccataattt
     1741 aaagattcaa atcgataaaa cgtttttgag cagaaacatt ccacgtttca ggattgttga
     1801 ttacttcagc aaatctttct gtgctggtgc gagtatctcc gccattaaag gtatcatctg
     1861 cttcaaattt gcctaaactg catgcttgtt gaatcgcatc aaagatattt ttagtttcat
     1921 aatctgtatg aataatagat tttcccatat ggcggttact ttggcgtgta ccaacatcaa
     1981 ttgaagggac accgtagaga ggagcttctc taatacctgc acttgagttg ccgaccataa
     2041 atttagcatg tttcaataag actaaaaaat attcaaatcg aatggaagga aatgcaataa
     2101 atttatcaga ttgatatttt aataattctt gcagaatact ttcagtgcca gtgtcattat
     2161 tagggtagat gctaatgata ttttggccac ttaattctaa tgctttgaaa tattgggccg
     2221 catattgtgg cattaaatgt gcttctgtag tcacggggtg aaacatagaa ataccataat
     2281 tttcgtatgg taaaccgtaa tattctttga cttcttctaa ggatgggagg gtggaagagg
     2341 ccataacatc taaatcgggg gagccgatga tgtgaatatg ctttcttttt tctcccattt
     2401 gcactaggcg agtgacagct tgttcatttg ctaccaagtg gatatgagaa agtttactaa
     2461 tagaatgacg aatggagtca tctactgtac cagatagttc accaccttcg atatggcaaa
     2521 ctaaacggct gcttaatgca cctacagctg cgcctgctag tgcttctaaa cggtcgccgt
     2581 gaatcatgac catatcaggt tcaatttcat cagatagacg agagataaac gtaatggtat
     2641 tgcctaaaac ggcacccatt ggttcacctt ggatttgatt tgaaaacaga tatgtatgtt
     2701 gatagttttc tcgagttact tccttgtagg ttctgccata tgttttcatc atatgcatac
     2761 cagttacaat caaatgcaat tcaaggtctg ggtgattttc aatataggct aataaaggtt
     2821 ttagcttgcc gaagtcggct ctggtacctg taatgcaaag aattcttttc atgattttag
     2881 aatctataag tatataagta tataagtata aggaagttgg aaagaagaat actaattata
     2941 ctctacgtac tcataaattt atttcgatta agtgctataa ttaggccatt tataattata
     3001 ttaggatttg gcttgtgttt aaagtgaaat tttatattcg tcacgcagta ttattattgt
     3061 gtggaagttt aattgtagga tgctctgcga ttccttcatc aggccccagc gcaaaaaaaa
     3121 ttgtctcttt agggcaacaa tctgaagttc aaattcctga agtggagctg attgatgtga
     3181 atcatacggt tgctcagtta ttatataagg ctcagataaa tcagtcattc actcagtttg
     3241 gcgatggtta tgcttcggct ggtacgctaa atattggtga tgtattggat attatgattt
     3301 gggaagcgcc gccggcagta ttgtttggtg gtggcctttc ttcgatgggc tcgggtagtg
     3361 cgcatcaaac taagttgcca gagcagttgg tcacggcacg tggtacggtt tctgtgccgt
     3421 ttgttggcga tatttcggtg gtcggtaaaa cgcctggtca ggttcaggaa attattaaag
     3481 gccgcctgaa aaaaatggcc aatcagccac aagtgatggt gcgtttggtg cagaataatg
     3541 cggcgaatgt gtcggtgatt cgtgctggga atagtgtgcg tatgccgctg acggcagccg
     3601 gtgagcgtgt gttggatgcg gtggctgcgg taggtggttc aacggcaaat gtgcaggata
     3661 cgaatgtgca gctgacacgt ggcaatgtag tacgaactgt tgccttggaa gatttagttg
     3721 caaatccgcg acaaaatatt ttgctgcgtc gcggtgatgt ggttaccatg attaccaatc
     3781 cctatacctt tacgtctatg ggtgcggtgg ggagaacaca agaaatcggt ttttcagcca
     3841 gaggcttatc gctttctgaa gccattggcc gtatgggcgg tttgcaagat cgccgttctg
     3901 atgcgcgtgg tgtgtttgtg ttccgctata cgccattggt ggaattgccg gcagaacgtc
     3961 aggataaatg gattgctcaa ggttatggca gtgaggcaga gattccaacg gtatatcgtg
     4021 tgaatatggc tgatgcgcat tcgctatttt ctatgcagcg ctttcctgtg aagaataaag
     4081 atgtattgta tgtgtcgaat gcgccgttgg ctgaagtgca gaaatttttg tcgtttgtgt
     4141 tctcgccggt taccagtggc gcgaacagta ttaataattt aactaattaa tgtgagtaat
     4201 taagatgtct gagcaacttc ctgtggcagt tgccactgaa accaaagccg agcgtaaaaa
     4261 gccgaaaaag aaaagttgga ttaaaaagct aagcccttta ttttgggtaa cggtgattat
     4321 ccctacggta atttcgttgg tgtatttcgg cttcttcgct tccgatcgtt ttacgtcgca
     4381 atcgagcttt gtggtgcgct cgcctaaaag ccaatcttct ctcaatggcc tgggtgccat
     4441 tttgcagggc acaggttttg cccgtgcgca agatgatatt tacacggttg gggagtatat
     4501 gcgttcgcgc tcgtctttgg atgaactgcg taaaatcttg ccggtgcgtg agttttatga
     4561 aaccaaaggt gatgcgttca gccgctttaa tgggtttggg ttccgtggcg aggaagaggc
     4621 tttttatcaa tactataaaa atcaggtgat gatcaatttt gatacggttt cgggtatttc
     4681 cacgttgaat gtaacttcct ttgatgcgct ggaatctaag aaaatcaatg aggctttgtt
     4741 aaaacaaggt gaagcattga ttaaccagtt gaacgatcgt gcacgtgctg atacggtgcg
     4801 ctatgcggaa gaagtagtga aaacggcggc agagcgggta aaggaagcct ctcagaatct
     4861 gacggattac cggattgcca atggcgtttt tgatttgaaa gcgcaatcgg aagtgcaaat
     4921 ggggttggtt tccaagctgc aagatgaatt gattgtgatt caaacccagc tggatcaggt
     4981 gaaagcagtc actccggaga atccgcagat tccgggtttg caggcgcgtg agcagagctt
     5041 gcgtaaagaa attgaccaac agttacgtgc catttcgggc ggtgggcatt cttcgttgtc
     5101 taatcaggct gccgaatatc agcgtgtgta tttggaaaac cagttggcag agcagcagtt
     5161 ggcagccgcc atgacttctt tggaaagtgc caaggttgaa gcagaccgtc agcagcttta
     5221 tttggaagtg atctcgcaac cgagcctgcc ggatttggca catgagccta aacggttata
     5281 caacattgtt gccactctga ttatcggctt gatggtttat ggtattttga gcctgttgac
     5341 tgccagcatt cgtgagcata aaaactgatg aaagccttgc ataaaacatc attttgggaa
     5401 tctttagcca ttcaaaggcg cgtaatcggt gcgctgttga tgcgggaaat tatcacccgt
     5461 tacggtcgca ataatattgg ctttttatgg ctgtttgttg agccgttgct gatgacattc
     5521 gttatcgtct tgatgtggaa atttttaagg gcagaccgat attcaacttt gaatattgtc
     5581 gcatttgcga ttactggcta tccgatgttg atgatgtggc gtaatgcctc aaaacgggca
     5641 gttgggtcga tttcttcaaa tgccagcttg ctttatcacc gcaatgtaag agttttggat
     5701 accatcttgg cgcgcatgat tttggaaatt gctggtgcaa ccattgcgca gattgtgatt
     5761 atggcggtat tgattgcgat tggctggatt gaaatgccgg cagatatgtt ttatatgctg
     5821 atggcttggc ttttgatggc tttttttgcg attggtttgg gtttggtgat ttgttcgatt
     5881 gcctttaatt tcgagccgtt tggcaagatt tggggcacat tgacttttgt gatgatgccg
     5941 ttatccggtg cgttcttttt tgtgcataat ttgccgccca aggtacaaga atatgcatta
     6001 atgattccga tggtgcatgg cacagaaatg ttccgtgccg gatattttgg cagcgatgta
     6061 attacctatg aaaatccttg gtatatcgta ttgtgcaatc tggtgttgtt gttgtttggc
     6121 ttggcgatgg tcagtaaatt cagtaaagga gtcgagccgc aatgatttca gttgaacacg
     6181 tttccaaacg ctatctgacc cgccaaggtt ggcggacagt cttgcacgat attagcttca
     6241 aaatggagaa gggcgagaaa atcggtattc tcggccgcaa cggtgcaggt aaatcgacgc
     6301 tcatccgttt gatcagtggt gtcgagccgc cgaccacggg tgaaatcaag cggacaatga
     6361 gtatttcttg gcctttggca ttctccggtg cgtttcaagg tagtctgacc ggtatggaca
     6421 atttgcgttt catctgccgg atttacaatg tcgatatcga ttatgtgaag aactttaccg
     6481 aagagttttc cgagttgggg caatatcttt acgagccggt caagcgttat tcttcaggta
     6541 tgaaggcgcg tttggcattt gctttgtcgt tggcggtaga gtttgattgc tatttgattg
     6601 acgaagtgat tgcagttggt gactcgcgct ttgccgataa atgtaagtac gagttgtttg
     6661 aaaagcgcaa agaccgttcc atcatcttgg tgtcgcacag ccacagcgcc atgaagcaat
     6721 attgcgataa tgcgatggtg ctggaaaaag ggcatatgca ccagtttgaa gatatggaca
     6781 aagcctacga atattataat tcgctgcctt aaagcgattg ttttttaaat caggccgtct
     6841 gaaatttcag acggcctgtc cgttggaatt ctattgatga acattactca aattctttcc
     6901 caagaacttt ccgcgactac cgcgcaaatc aatgccgcta ttgagctttt agacgacggc
     6961 gcaaccgtgc cgtttatcgc gcgttaccgt aaagaagcca ccggcgggct ggacgacacg
     7021 cagctgcgcc ggcttgccga gcggctgcaa tacctgcgcg agttggaaga gcgcaaagcc
     7081 gttgtgttaa aaagcattga agagcaaggc aagctttcag acgacctcag ggcgcaaatc
     7141 gaagccgccg ataacaaaac cgcgctggaa gacctgtatc tgccctacaa acccaaacgc
     7201 cgcaccaaag cgcaaatcgc gcgcgaacac ggtttgcagc cgctggcgga cgtgttgctt
     7261 gccgagcagt cgcaggacgt ggaagccgcc gcacaaggct acctgaacga aaacgtcccc
     7321 gatgccaaag ccgcgttgga cggcgcgcgt gcgattctga tggagcagtt tgccgaagac
     7381 gcggaactca tcggcacgct gcgcgacaag ctgtggaacg aagccgaaat ccacgcgcaa
     7441 gttgttggag gcaaagaaac cgaaggcgaa aaattcagcg attattttga ccaccgcgaa
     7501 cctattcgtg cgatgcccag ccatcgtgcg ctagcggttt tgcgcggacg caacgaaggc
     7561 gtgttgaaca tcgcgctcaa atatcagccc gacgacacgc cgattacgca gcaaagcgaa
     7621 tacgagcaaa tcatcgcctg ccgcttcaag gtttcagacg gccacaaatg gctgcgcgat
     7681 accgtgcgtc tgacttggcg cgcgaaaatc tttttgtcgt tggaacttga agccctaggc
     7741 cgtctgaaag aagccgccga caccgacgcg attaccgtgt tcgcccgcaa tctcaaagac
     7801 ttgctgctcg tcgcgcccgc cggacggctg accacgctgg gtctcgaccc cggctaccgc
     7861 aacggcgtga aatgcgccgt ggtggatgat acgggcaagc tgctggatac cgtcatcgtc
     7921 tatttgcatc aagaaaacaa tatgttggca acgctgtcgc gcctgattaa acagcacggc
     7981 gtgaagctca tcgccatcgg taacggcacc gccagccgtg aaaccgacaa aatcgcgggc
     8041 gaactggtgc gtgaaatgtc gaaaatgggg ctgcataaaa tcgtcgtgtc cgaagccggc
     8101 gcgtcgattt attccgcgtc cgaactggcg gcgcgcgagt tccccgactt ggacgtttcc
     8161 ctgcgcggcg cggtgtccat cgcccgcagg ctgcaagacc cgcttgccga gttggtcaaa
     8221 atcgacccca aatccatcgg cgtggggcag tatcagcacg acgtgaacca aagccagctc
     8281 gccaaatcgc tggacgcggt ggtcgaagac tgcgtgaacg ccgtcggcgt ggacgtgaac
     8341 accgcctccg ccccgctctt ggcgcgaatt tccggtttga atcaaaccct tgcccaaaac
     8401 attgtcgcct accgcgatga aaacggcgcg ttcgacagcc gcaaaaaatt gctgaaagta
     8461 ccgcgtttgg gcgaaaaaac cttcgagcag gcggcgggct ttttgcggat taacggcggc
     8521 aaagagccgt tggacgcgag cgccgtccac cccgaagcct atcccgtcgt cgccaaaatg
     8581 ctggcgcaac aaggcattag cgccgccgaa ctcatcggca accgcgagcg cgtgaagcaa
     8641 atcaaagcgt ccgacttcac cgacgaacgc ttcggcctgc cgaccatttt ggacatcctg
     8701 tccgaactgg aaaaacccgg ccgtgatccg cgcggcgagt ttcagacggc atcgtttgcc
     8761 gaaggtatcc acgaaatcag cgacttgcaa gtcggtatga tactcgaagg cgtggtttcc
     8821 aacgtcgcca acttcggcgc gttcgtggac atcggcgtcc atcaggacgg cttggtgcac
     8881 atctccgccc tgtccaacaa gttcgtccaa gacccgcgcg aagtggtgaa agctggcgac
     8941 gtggtgaaag tgaaagtgct ggaagtcgat gctgcacgca aacgcatcgc gctgaccatg
     9001 cgcttggatg acgaaccggg cggcgcaaaa cataaaatgc cgtctgaaaa ccgcagccgc
     9061 gaacggacag ccggccgcaa accccaacgc aacgaccgcg ccccagccaa ttcggcgatg
     9121 gcggatgcgt ttgcgaagct gaagcggtaa aataatcgaa gagtttatgg attttgactt
     9181 atgcacacac cacttaccta tattgacctt ttctcaggag caggaggcct atccttgggt
     9241 tttgaacaag ccggattcca acaattgctt tctgttgaaa tggagtctga ttattgtcag
     9301 acttaccgta ccaacttccc ccatcatcaa ttactgcaaa aagatttaac cacactaacc
     9361 gaacaagatt taatcaattg tcttaacgga caagcagttg atttgattat tggaggacca
     9421 ccttgtcaag gttttagtat ggcaggaaag attggacgga catttacaga tgacccacgc
     9481 aaccatttat ttaaagagtt tgtccgaata gttaaaattg tccaaccata tttttttgtt
     9541 atggaaaatg tagcgcgact ctatacacac aattcaggta aaacacgtat tgagattatt
     9601 caagcatttc agaatatcgg ttattcggtg gaatgtaaga tactgagtgc agccgatttc
     9661 ggtgttcctc agatacgtag ccgagtgata tttatcggga ggagggataa aggcaaaatt
     9721 tcctttcccg aacctttgca gatttcccat cagactgttg gatcagcaat aggacatttt
     9781 ccaaaactgg ctgctggcga aagcaatcca cacgttgcaa atcatgaagc tatgaatcat
     9841 tcggcacaaa tgttagaaaa aatggcattt gttaaaaatg gaggtaaccg taacgatatt
     9901 cctgaaccat tacgtccgaa aacaggtgat atccgtaaat acatccgtta caacagcaac
     9961 aaaaccagcc gtttgtatta caggagatat gcgcaaagtt tttcactatg aacagaatcg
    10021 ggcgttaacc gttcgtgaat tagctgcctt acaatctttc cctgataatt ttattttttg
    10081 cggcagcaaa attgcccagc agcagcaggt tggtaacgcc gtaccgcctt tattggcaaa
    10141 agctattgct gaaagtattt taaaaatgag tgaaaatgaa taagcaatat ccgaaaatta
    10201 actatatcgg taataaagag aaaatagctt cctggatttg tgaccagctt ccgtctgatg
    10261 tagatacagt tgcagatgta tttagtggag gctgttcctt tgcctacgaa gccaaaaaac
    10321 gcggctatcg tgtgattact aacgatattt tggcaattaa ttaccaaatt gctttagcat
    10381 taatagaaaa caaccatgaa acattaaatg acgatgatgg cgcaatgatt ttttcaggta
    10441 gcccgcatgc cggttttatg agtcagcgtt atgccgaaaa attctatttt cacgatgaat
    10501 accaacaact tgatttgtaa cgtaaaaata tagggaaact ggataaccag tataaacgcg
    10561 ctttggcgtt tactttaatg cgtcgcgcca tgatacgtaa aatgccctat acggaagata
    10621 tgcgcccggg cgataccgct aatccttatg gcgcatccaa agcgatggtg gaacggatgt
    10681 taaccgacat ccaaaaggcc gatccgcgtt ggagcgtgat tttgttgcgc tatttcaacc
    10741 cgattggggc gcacgaaagc ggcttgatcg gtgaacagcc caacggtatt cccaacaacc
    10801 ttttgcccta tatctgccaa gtggcttcgg gcaggctgcc gcaactgtcg gtattcggcg
    10861 gcgattatcc gaccccggac ggtacgggaa tgcgcgacta tattcatgtg atggatttgg
    10921 cagaagggca tatcgcggca atgaaggcga aaggcggtgt tgccggcgta catttgttca
    10981 acttgggttc ggggcgcgcc tattcggttt tggaaatcat ccgtgccttt gaagccgcat
    11041 ccggcttaaa tattccctat caaatccaac cccgacgcgc cggagacttg gcgtgttcct
    11101 ttgccgaccc gtcccatacc aaacaagaga caggttggga aaccaaacgc agccttgcgc
    11161 aaatgatgga ggattcgtgg cgttgggtca gccgcaaccc caacggatat ggggattaaa
    11221 tgcaaaccgc aaacaaaaaa accatcctcg ttaccggcgg cgcgggcttt atcggctctg
    11281 ccgttgtccg ccatatcatt caaaacaccc aagattccgt cgtcaacctc gacaaactga
    11341 cctacgcggg caatctcgaa tcgctgaccg acattgccga taatccccgc tacgcttttg
    11401 agcaagtgga tatttgcgac cgcgccgaac tcgaccgcgt attcgcacaa caccggcccg
    11461 atgccgtgat gcacttggca gcggaaagcc atgtcgaccg ctctatcgat tcggcaggcg
    11521 agtttatcca aaccaatatc gtcggcacat tcaacctact tgaagctgcg cgtgcctacc
    11581 ggcagcaaat gccgtctgaa aagcacgaag ccttccgttt ccaccatatt tctaccgatg
    11641 aagtttatgg cgatttaagc ggcacagacg atttgtttac cgaaaccgcg ccctacgcgc
    11701 cgtccagccc ttactctgcc tctaaagcgt ccagcgacca cctcgtccgc gcgtggttgc
    11761 gtacttacgg cttgccgacc attgtaacca actgctccaa caactacggt ccttaccatt
    11821 ttccggaaaa actcattcct ttgatgattc tgaacgcgct tgacggcaaa ccgctgcctg
    11881 tgtacggcga cggtatgcaa atccgcgact ggctgtttgt cgaagaccac gcgcgcgcac
    11941 tgtatcaggt tgttaccgaa ggtgttgtcg gcgaaaccta caatatcggc ggccacaatg
    12001 aaaaagccaa tattgaagtc gtcaaaacca tctgcgccct gctggaagaa ctcgctcccg
    12061 aaaaaccggc cggcgtggcg cgttatgaag atttgattac tttcgtacaa gaccgccctg
    12121 gccacgatgc ccgctacgcc gtcgatacgg cgaaaatcag acgggatttg ggctggcagc
    12181 ctttggaaac ctttgaatcc ggcctccgca aaacagtgca atggtacttg gacaacaaaa
    12241 ctcggcggca aaacgcataa aacggcagct atcgtttgga acgtttaggc accggaaaat
    12301 aagttttcag acggcatccc gacgcaatgc catctgaaaa cccatcgcaa aggaagaaag
    12361 aaaagatgaa aggcatcata ctggcaggcg gcagcggcac gcgcctctac cccatcacgc
    12421 gcggcgtatc caaacagctc ctgcccgtgt acgacaaacc gatgatttat taccccttgt
    12481 cggttttgat gctggcggga atccgcgata ttttggtgat taccgcgcct gaagacaacg
    12541 cctctttcaa acgcctgctt ggcgacggca gcgatttcgg catttccatc agttatgccg
    12601 tgcaacccag tccggacggc ttggcacagg catttatcat cggcgaagaa tttatcggca
    12661 acgacaatgt ttgcttggtt ttgggcgaca atatttttta cggtcagtcg tttacgcaaa
    12721 cattgaaaca ggcggcagcg caaacgcacg gcgcaaccgt gtttgcttat caggtcaaaa
    12781 accccgaacg tttcggcgtg gttgaattta acgaaaactt ccgcgccgtt tccatcgaag
    12841 aaaaaccgca acggcccaaa tccgattggg cggtaaccgg cttgtatttc tacgacaacc
    12901 gcgccgtcga gttcgccaaa cagctcaaac cgtccgcacg cggcgaattg gaaatttccg
    12961 acctcaaccg gatgtatttg gaagacggct cgctctccgt tcaaatattg ggacgcggtt
    13021 tcgcgtggct ggacaccggt acgcacgaaa gcctgcacga ggcggcttcg ttcgtccaaa
    13081 ccgtgcaaaa catccaaaac ctgcacatcg cctgcctgga agaaatcgct tggcgcaacg
    13141 gttggctttc cgatgaaaaa ctggaagaat tggcgcgccc gatggcgaaa aaccaatacg
    13201 gccaatattt gctgcgcctg ttgaaaaaat aatgtttgag gccgtctgaa acttttcaga
    13261 cggcctttag atgaaagata aaaagatgaa catcattgat accgccattc ctgacgtaaa
    13321 actgcttgag ccccaagtct tcggcgacgc gcgcggcttt tttatggaaa ccttccgcga
    13381 cgagtggttt aaaacccaag tctgcgaacg caccttcgtg caggaaaacc actccaaatc
    13441 cggcaaaggc gtattgcgcg gcctgcacta ccaaaccgaa aacacacaag gcaaactcgt
    13501 acgcgtagtt gtcggcgaag tgttcgacgt ggccgtcgat atgcgcgaag gttcgccgac
    13561 tttcggcaaa tgggcgggcg caaccctgtc cgcgcaaaac caatgccagc tttggatacc
    13621 cgaaggcttc gcgcacgggt tttgcgtttt gggcgacgcg gcggaagtcg tgtacaaatg
    13681 caccgactat tacaagcctg aagccgaaca ggttttaata tggaacgacc cgacagtcgg
    13741 cataggctgg ccgcttcaaa ccgcgccgct gctgtcgccc aaagaccttg ccggcaaaac
    13801 gtgggcgcaa gccgaaaagc tccgccttcc gctttccaga taaaaatgcc gtctgaacgc
    13861 ttcagacggc attttttccg acagcctact tgcccgcctt cagtacgcgc tgtgcaaaga
    13921 aaaacatccc ggtaacgaag aacgccaaac ccaaccaaga agcggcggca tcccagtttt
    13981 gcagattcaa cgcaagcggc gcatccgagc cgccgttggt cacggagaag gcaataataa
    14041 acgccataat cacaccgatc aggctttcac cgacaatcag gccggcggag aacaaggttc
    14101 cgatacgctc ggcgtttttc agacggcctt cgcggttttc cgctttttta ccgatgatgt
    14161 gtttcaacac cgccgccaac accgcgcctg ccacgatggg catattgacg gacggcggca
    14221 gataaatacc cataccgacc gcaaggacgg gcagggcaag tttgccgcct gatgattttt
    14281 tcaacaccaa atcgacgacg attaataccg cgccgatcgc gatgccggta aagatgtaga
    14341 cccattcgag gttgtgggcg aaaatgcccg acgcgatggt cgtcatcaaa gtcgcttgag
    14401 gagctgccaa agcctgcgcc gtatccatgc cttcgcgcgg cattgcgccg gtaaagccgt
    14461 aggcttcgta aagcagttcc aacacgggcg aaataaccag cgcaccaacg atacagccga
    14521 taatcagggc gacttgctgc cgccaaggcg tggctttgag caggtagccg gttttcaagt
    14581 cttgcaggtt gtcattggaa atcgaagcca cgcagattac tgccgagccg caaaacaaag
    14641 tcagtgccag caaaaatttg cggttagcct catccgccaa caaacctccg gattcgccta
    14701 ccagcagcaa aaccagtgaa ataacgacga cggacacgat gcccacgccg gaaatcgggc
    14761 tggaagacga gccgaccaaa cctgccatat aaccgcaggc ggcggcgacc aaaaagccga
    14821 tgacggaagc caaaagcgtg caaacgacca ccaaaagcca agccatgccg cccgtaatgt
    14881 gcgaatcgcc gataaagtgg taaaacgaca cgcctaaaac aaacatcatc gccagcaccc
    14941 aaaaaatcat ggctttaggc gacaaatctt gttcggcgcg ttccgcagcg ggcgcaccgc
    15001 cgccaaaact cttgaacgac atcttcatgc cttccaccat cggcttgagc agcatcaaca
    15061 gcgtccaaac cgccgcaatg ccaatagtcc ccgcaccgat aaaacgcact ttctccttcc
    15121 acagcttcat cgcaaacgcc gccatttcca tatcggaagg ttgcggaatg tgtgaggaga
    15181 aatacggcac ggcaatgccc caagcaatcg aaatgcccaa caggatggcg ataccgcccg
    15241 tcagtccgac caaatagccc gcgcccaaca atgccagtga aaagcccatc ggcagctgga
    15301 aaatcgccgt accgctttta aaccaataac tcgcgctgtc ggcaatcacg cgcagacctc
    15361 cggcgcaaaa gctcatcaat cccgccaacg caccgccggc cgccagctct ttgatgccgc
    15421 tgccgccctg acggttatcc ccttcttcat gaccgcccac tttcaaaatt tcagcagccg
    15481 ccacaccttc cggataaggc aaatcgcttt tcaccaccat cgcgtaacgc agcggaatgg
    15541 tgaaaatcac ccccaaaatc ccgccggcaa tacataaaag cgtcgtctgc cagaacggga
    15601 aaccgctcca gtagcccgcc atcagcaaac cgggcaggac gaagatgatg gtcgaaagcg
    15661 tacccgccgc cgaagcttgg gtctgcacca tgttgttttc caaaatattg ctgcctttga
    15721 aaaactttaa aaccgccatc gaaatcaccg ccgccggaat cgacgaggca aaggtcagcc
    15781 cgactttcaa accgaggtaa acattggacg cagtaaaaat tacagtgatc aatgcaccga
    15841 gtatcatgcc tcggagcgtc agctcgcggt attcttctac cgaactggat aaagatttat
    15901 tcattattct tcctttgaca acagacgttc acatattgtt ggcatcacgc catgatgtca
    15961 agttttaaaa agaacagtta aaaacagtta tcccaccctg cctcataccc cattgaaaat
    16021 aaaaactatt tttaaacaaa taaaaacagc cgtatcaagg agattccccg atacggctgc
    16081 ttgtttccga accttaaaat caatcaaaca aatcgcgcag cttgtctaaa aacgatttct
    16141 tgcgcggtgt ttggttttcc aagccggtag aaatccgctc aaactcttcc aaaagctctt
    16201 tttgacggtc agtcaaattg acaggcgttt cgacaacaat atggcagtac aaatcgccgg
    16261 tcgcgctgct gcgcaacgat ttgacaccct tacctttcac acgcatcctc ctgccggttt
    16321 gggtttcttt ggggacggtg agcttgacct ttccgtccaa ggtcggcact tccaactccc
    16381 cgcccaaagc agccgtggca aaactgatcg gcagttcgca atgcaagtcc agaccgtcgc
    16441 gttggaaaat cttatgcgcc cgaatgcgga cggttacata caagtcgccg gcaggcgcac
    16501 cgtgcatacc cggcccgcct tcgccgctca aacggatacg ctgcccgtca tcgataccgg
    16561 cgggaatatt gacttccacc gtcttgaccg ccttattccg ccccgcgcca cggcatttga
    16621 cgcaaggttc tttaatgtgt ttgcccgcac cgtggcaggt cggacaagtc tgctgcatac
    16681 ggaaaatcgc ctgctggatg tgcaccgtac ccgaaccttt gcaagtcggg caggtttccg
    16741 gggatgtccc cggtttcgcg ccactgccgt tacagacatc acacgcttca taagtcggaa
    16801 tattgatgcg tttcttcaca ccttttgcgg cttcttcaag cgtgatttcg ataccgactt
    16861 gaacgtcctc accctgataa tcaggctggg cgcgccccga accgcctcca aacatttggc
    16921 tgaaaatatc cccaaagtca aaaccctgcg caccgccaaa tccgccaaac cctccgaagc
    16981 ccccctgtcc gccgccttca aacgccgcat gaccatactg gtcgtacata gcgcgctttt
    17041 ccttgtcgga caaagtttca tacgcctttt gtacttcttt aaacttctct tccgcctctt
    17101 tattgtcagg attgcggtcg ggatggtatt tcatcgccaa tttccggtag gcttttttaa
    17161 tctcatcatc ggtagctgtt cttgccacac ccagcgtcgc ataaaaatct tgattactca
    17221 ttttttcatc taattcaaaa taaaatcacg gctcaaaata agggcaatta cgcaaaacac
    17281 aagacaaaca gactgccata gcttacaaac tgaaacggaa tacacttttc agacagcata
    17341 aaccgatgcc gtctgaaatc ctcaggtatg cacgacacaa aaacttagac aggcataaaa
    17401 ccaaccgcca tgaagtgttt ttgttataaa aacgccgccc gaacgcatgt tcagacggca
    17461 tttcctttgc gtcgattcat tccccgatat agcgcaggtc tttttc
//
LOCUS       DAXHTR010000027        17433 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.027, whole genome shotgun sequence.
ACCESSION   DAXHTR010000027 DAXHTR010000000
VERSION     DAXHTR010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.027"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            241..1305
                     /locus_tag="ACFNML_000645"
     CDS             241..1305
                     /locus_tag="ACFNML_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HFC6392061.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGPGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKEGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            1446..1769
                     /locus_tag="ACFNML_000646"
     CDS             1446..1769
                     /locus_tag="ACFNML_000646"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HFC6392062.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            1841..2164
                     /locus_tag="ACFNML_000647"
     CDS             1841..2164
                     /locus_tag="ACFNML_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HFC6392063.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            2211..2414
                     /gene="tatA"
                     /locus_tag="ACFNML_000648"
     CDS             2211..2414
                     /gene="tatA"
                     /locus_tag="ACFNML_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFC6392064.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            2418..3104
                     /gene="tatB"
                     /locus_tag="ACFNML_000649"
     CDS             2418..3104
                     /gene="tatB"
                     /locus_tag="ACFNML_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFC6392065.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANPASDGISDVMPSERSYTSAETLGDDRQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRDLRPKSR
                     AKPKLRVRKS"
     gene            3118..3888
                     /gene="tatC"
                     /locus_tag="ACFNML_000650"
     CDS             3118..3888
                     /gene="tatC"
                     /locus_tag="ACFNML_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HFC6392066.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGLMPFAQQ
                     LYTFIADPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLASVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTEQLKRARPYVIVGAFVIAAVITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIQPPATT"
     gene            3941..4546
                     /locus_tag="ACFNML_000651"
     CDS             3941..4546
                     /locus_tag="ACFNML_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HFC6392067.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAATFLNLL
                     SGTGHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIEGSFSGIMGLPVYETVSMLQDLGYRTPPFIRA"
     gene            4562..4915
                     /locus_tag="ACFNML_000652"
     CDS             4562..4915
                     /locus_tag="ACFNML_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HFC6392068.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            4905..6719
                     /locus_tag="ACFNML_000653"
     CDS             4905..6719
                     /locus_tag="ACFNML_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wzy polymerase domain-containing protein"
                     /protein_id="HFC6392069.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALKLKPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWACKSLVAHFGQERIVTLFAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNLIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPPALGAICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVLLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     NIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            6909..7586
                     /gene="misR"
                     /locus_tag="ACFNML_000654"
     CDS             6909..7586
                     /gene="misR"
                     /locus_tag="ACFNML_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator MisR"
                     /protein_id="HFC6392070.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            7599..9005
                     /gene="misS"
                     /locus_tag="ACFNML_000655"
     CDS             7599..9005
                     /gene="misS"
                     /locus_tag="ACFNML_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     MisS"
                     /protein_id="HFC6392071.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            9123..11549
                     /locus_tag="ACFNML_000656"
     CDS             9123..11549
                     /locus_tag="ACFNML_000656"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFC6392072.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGNADLIIAVTLPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLHPGISPITSADWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHSLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKNLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALAAANPSLLAEAL
                     HLPPYRHTTHNAVQFQFDSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     YPKTLSLRNNTTVTITPILPEDAEAKQQFVRSLGPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLRTDHKIT"
     gene            11594..11839
                     /gene="rpsP"
                     /locus_tag="ACFNML_000657"
     CDS             11594..11839
                     /gene="rpsP"
                     /locus_tag="ACFNML_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFC6392073.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            11855..12364
                     /gene="rimM"
                     /locus_tag="ACFNML_000658"
     CDS             11855..12364
                     /gene="rimM"
                     /locus_tag="ACFNML_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFC6392074.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISVTLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     TDLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKI
                     ITADWGLDY"
     gene            12364..13113
                     /gene="trmD"
                     /locus_tag="ACFNML_000659"
     CDS             12364..13113
                     /gene="trmD"
                     /locus_tag="ACFNML_000659"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFC6392075.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQAAKVIYLSPQGKPLTHPKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRVLIPKESRLLETIRQEQREIQS"
     gene            13129..13494
                     /gene="rplS"
                     /locus_tag="ACFNML_000660"
     CDS             13129..13494
                     /gene="rplS"
                     /locus_tag="ACFNML_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFC6392076.1"
                     /translation="MNLIQQLEQEEIARLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            13815..14540
                     /locus_tag="ACFNML_000661"
     CDS             13815..14540
                     /locus_tag="ACFNML_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HFC6392077.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            14575..15450
                     /locus_tag="ACFNML_000662"
     CDS             14575..15450
                     /locus_tag="ACFNML_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HFC6392078.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            15527..16441
                     /locus_tag="ACFNML_000663"
     CDS             15527..16441
                     /locus_tag="ACFNML_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HFC6392079.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALINAMKQ"
     gene            complement(16721..>17433)
                     /locus_tag="ACFNML_000664"
     CDS             complement(16721..>17433)
                     /locus_tag="ACFNML_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6392080.1"
                     /translation="LVQQSTDGSDRLYAYQSGNTLNGGLGDDYLYGADGDDLLNGDAG
                     NDSIYSGNGNDTLNGGEGNDALYGYNGNDVLNGGEGNDHLNGEDGNDTLIGGAGNDYL
                     EGGSGSDTYVFGKGFGQDTVYNYHVDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQ
                     DNVRISGFFYGENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGN
                     VDANTQSVQQPLLVTPSA"
BASE COUNT         4553 a         5525 c         3809 g         3546 t
ORIGIN      
        1 ccgtactggt ttttgttaat ccactataaa tgccatctga agccgattcg gttttcagac
       61 ggcatcatta tttgacctga cgcaaacaga aagccttccg cgtgcttaaa cacatctctt
      121 gtagtaaaat atctaaacag attcaactgg aaacggtcag gcttcttcac gccattccca
      181 tttcaaccgc cgcctgccag attcagacgg tattcctact tgaaaacaag gagcgttaca
      241 atgaaagcag cacgttttta cgacaaaggc gacatccgca tcgaagacat ccccgaaccg
      301 accgtcgccc ccggcaccgt cggcatcaat gttgcctggt gcggcatctg cggtaccgac
      361 ctgcacgaat tcatggaagg cccgattttc attccgcctt gcggtcatcc gcacccgatt
      421 tccggcgagt ccgcacccgt cacgatggga cacgagttct ccggcgtggt ctatgccgtc
      481 ggcgaaggcg tggacgacat caaagtcggt caacacgtcg ttgtcgagcc ttacatcgta
      541 cacgacgacg taccgaccgg cccgggcagc aactaccacc tctctaaaga catgaacttc
      601 atcggcttgg gcggctgcgg aggtggcttg tctgaaaaaa tcgctgtcaa acgccgttgg
      661 gtgcatccga tttccgacaa aatcccgttg gatcaagccg ctttgatcga accgctgtct
      721 gtcggacacc acgcctatgt acgcagcggc gcgaaagaag gcgatgtcgc attggtcggc
      781 ggtgcaggcc cgatcggttt gctgttggct gccgtgctga aagccaaagg catcaaagtc
      841 atcatcaccg agttgagtaa agcacgcaaa gacaaagcgc gcgaatccgg cgttgccgac
      901 tacatcctcg acccgtccga agtcgatgtt gtcgaagaag tgaaaaaact gaccaacggc
      961 gaaggcgtgg acgtggcatt cgaatgtacc agcgtgaaca aagtgttgga tactttggtc
     1021 gaagcctgca aacctgccgc caatttggtt atcgtatcca tctggagcca ccctgcaacc
     1081 gtcaacgtcc acagcgtcgt gatgaaagag ctggatgtgc gcggcacgat tgcctactgt
     1141 aacgaccacg ccgaaaccat caaactggtc gaagaaggca aaatcaacct cgaacccttc
     1201 atcacccagc gcatcaagct ggacgaactg gtttccgaag gcttcgagcg tctgatccac
     1261 aacaacgaat ccgccgttaa aattattgtg agtccaaacc tgtaagcaaa ttacatttgc
     1321 aacagcacaa atgccgtctg aaacgctcag acggcatttt ccaacgaagc gcaacaggcg
     1381 tattgcccga gagcagccgt ttagcctatc attccgaaca aacagaaaca aacagaagga
     1441 aaatcatggg agattccgta ctatccgcca tccaacaaac catcacccag cgaaaatctg
     1501 ccaatccgtc cgaatcttac gtcgcacagc tcttgcataa gggcgaagac aaaatcctaa
     1561 aaaaagtgat tgaagaggcg ggcgaagtgt tgatggcatc caaagacaaa aacccgtccc
     1621 acctggttta cgaagttgcc gacttatggt ttcacaccat gattcttctg acacaccacg
     1681 acctgaaggc ggaagacgta ttggacgaac ttgcgcgccg ccaaggtttg tcgggcttgg
     1741 ccgaaaaagc cgctcgcaca gaatcttgaa tttatattaa aatccgcact ttcccacatt
     1801 caatccgtct gaccgctttt cagacggcat cggagccgtt atggacaact gtattttctg
     1861 caaaatcgcc gccaaagaca ttccggcgca aaccgtctac gaagacggcg aaatggtttg
     1921 tttcaaagac atcaaccccg ctgctccggt tcatctgctg ctgattccca aagtccattt
     1981 cgattcgttg gcacacgccg cgcccgaaca tcagcccctt ttgggaaaaa tgatgctgaa
     2041 agttcccgaa atcgccaaag cggcaggact ggcagacggc ttcaaaaccc tgatcaacac
     2101 cggaaaaggc ggcggacaag aggtcttcca cctgcatata cacatcatgg gcacacccgt
     2161 ataaaccgtt atttcacaat caacccctaa tacttactta aggatacatc atgggcagtt
     2221 tttctctgac gcactggatt atcgtactga ttatcgtcgt tttgatattc ggcaccaaaa
     2281 aactgcgcaa cgtcggcaaa gacctcggcg gtgcggttca tgacttcaaa caggggctga
     2341 acgaaggcac agacggcaaa gaagcccaaa aagacgatgt aatcgaacac aaaaaagacg
     2401 aagacaaagc gtaatttatg tttgatttcg gtttgggcga gctggttttt gtcggcatta
     2461 tcgccctgat tgtcctcggc cccgaacgcc tgcccgaggc cgcccgcacc gccggacggc
     2521 tcatcggcag gctgcaacgc tttgtcggca gcgtcaaaca ggaatttgac actcaaatcg
     2581 aactggaaga actgaggaag gccaagcagg aattcgaagc tgccgccgct caggttcgag
     2641 acagcctcaa agaaatcggt acggatatgg agggtaatct gcacgacatt tccaatggtc
     2701 tgaagccttg ggaaaaactg cccgaacagc gcacgcctgc tgatttcggt gtcgatgaaa
     2761 acggcaatcc gcttcccgat gcggcaaacc ccgcatcaga cggcatttcc gacgttatgc
     2821 cgtccgaacg ttcctacact tccgccgaaa cccttggaga cgacaggcaa accggcagta
     2881 cagccgaacc cgcggaaacc gaccaagacc gcgcatggcg ggaatacctg actgcttctg
     2941 ccgccgcacc cgtcgtacag accgtcgaag tcagctatat cgataccgct gttgaaaccc
     3001 ctgttccgca taccacttcg ctgcgtaaac aggcaataag ccgcaaacgc gatttgcgtc
     3061 ccaaatcccg cgccaaacct aaattgcgcg tccgtaaatc ataaagaggg caatccggtg
     3121 tccgaaacac aaaacgaaca acccgtccaa ccgcttgtcg agcatctcat cgagctgcgc
     3181 cgccgcctga tgtggacggt tgtcggcatc ttagtctgct ttttcggcct aatgccgttt
     3241 gcccaacaac tctatacttt tatcgccgac ccgctgatgg caaacctgcc caaagacacc
     3301 agcatgattg ccaccgatgt catcgcaccg tttttcgtgc cggtcaaagt taccctgatg
     3361 gcggcatttt taatttcgct gccgcatacg ctctaccaaa tctgggcatt tgtcgcgccc
     3421 gcactctacc aaaacgaaaa acgcctgatt acgccgctcg tcctctccag cgtcagcctg
     3481 tttttcatcg gcatggcatt tgcctacttt ttggttttcc ccgtcatttt caaattcctt
     3541 gccagcgtta cccctgtcgg tgtcaatatg gcgacagaca tcgacaaata cctctccttc
     3601 atcttgggga tgtttgtcgc attcggtaca acgtttgaag tccccattgt cgttatcctg
     3661 ttaaccaaaa ttggtgtggt aacaaccgaa cagctcaaac gcgcccgccc ctatgtgatt
     3721 gtcggcgcgt ttgtcattgc cgcagtcatc acaccgcccg atgtgatttc acaaaccctg
     3781 cttgccattc cgctgattct cttatacgaa gcaggtattt ggttcggacg ctttttcacg
     3841 ccacgttcag aacaggatgg cgacatacag ccgcctgcaa caacctgaca ctatgccgtc
     3901 cgaacctccg cctcataccg caacagatta aggaatacct ttgaataccc tctatttagg
     3961 ttcaaacagc ccgcgccgaa tggaaatcct gacacagttg ggctatcagg tcgtcaagct
     4021 gcctgccgac atcaatgaaa cggtcagaca gaacgaagac cctgcccgtt acgttcaaag
     4081 gatggcagaa gaaaaaaacc gaaccgccct gaccctcttt tgcgaaacca acggcacaat
     4141 gcccgatttc cccctgatta ccgccgacac ctgcgtcgtt tcagacggca tcatattggg
     4201 caaaccccgc tcccaagctg aagcggcaac ctttctaaac ctgctgtcgg gaaccgggca
     4261 taccgtcctg actgctgtct gcattcatta tcgcggcaaa acgtcaagcc gcgtccaaac
     4321 caaccgcgtc gttttcaagc ccctgagttc ggaagaaatt tccgcctatg tccaaagcgg
     4381 cgaaccgatg gacaaagccg gcgcctacgc cgtacaaggc ataggcggca tctttatcca
     4441 atctatcgaa ggcagcttca gcggcattat ggggctgccc gtttatgaaa ccgtttcgat
     4501 gttacaggat ttgggatacc gtaccccacc tttcatccga gcctaacccg taaagaccgc
     4561 cgtgaacaga caaaccgctt acctccttac ctctttcagc ctgatcgcac tgataatcct
     4621 gtccctttcc tgggaactgt ggattgcacc gttgcgcccg ggcggctcgt ggctcgcgct
     4681 caaagccctc cccctctgtc tgccgctttc aggcatcttg aaaaagaaaa tctataccta
     4741 ccaatacagc tccatgctgg ttctgattta ctttgccgaa gccgtcatgc gcctgttcga
     4801 cgcctatccc gcagaacaga tttgcgccgc gctttccgca gtattcagca tcatcttctt
     4861 catatcctgc ctgtcgttcg tcaaacaata caaggaaaca aacaatgccc gctgaaacga
     4921 ccgtatccgg cgcgcacccc gccgccaaac tgccgattta catcctgccc tgcttccttt
     4981 ggataggcat cgtccccttt accttcgcgc tcaaactgaa accgtcgccc gacttttacc
     5041 acgatgccgc cgccgcagcc ggactgattg tcctgttgtt cctcacggca ggaaaaaaac
     5101 tgtttgatgt caaaatcccc gccatcagct tccttctgtt tgcaatggcg gcgttttggt
     5161 atcttcaggc acgcctgatg aacctgattt accccggtat gaacgacatc gtctcttgga
     5221 ttttcatctt gctcgccgtc agcgcgtggg cctgcaagag tttggtcgca cacttcggac
     5281 aagaacgcat cgtgaccctg tttgcctggt cgctgcttat cggctccctg cttcaatcct
     5341 gcatcgtcgt catccagttt gccggctggg aagacacccc cctgcttcaa aacctcatcg
     5401 tttacagcgg gcaaggcgta atcggacaca tcggacagcg caacaacctc ggacactacc
     5461 tgatgtgggg catactcgcc gccgcctacc tcaacggaca acgaaaaatc ccgcccgcct
     5521 tgggcgcaat ctgcctgatt atgcagaccg ccgttttagg tttggtcaac tcgcgcacca
     5581 tcttgaccta catagccgcc atcgccctca tccttccctt ctggtatttc cgttcggaca
     5641 aatccaacag gcggacgatg ctcggcatag ccgcagccgt actcctaacc gcgctgttcc
     5701 aattttccat gaacaccatt ctggaaacct ttaccggcat ccgctacgaa actgccgtcg
     5761 aacgcgtcgc caacggcggt ttcacagact tgccgcgcca aatcgaatgg aataaagccc
     5821 ttgccgcctt ccagtccgcc ccgatattcg ggcacggctg gaacagtttt gcccaacaaa
     5881 ccttcctcat caatgccgaa cagcacaaca tacacgacaa cctcctcagc aacttgttca
     5941 cccattccca caacatcgtc ctccaactcc ttgcagagat gggaatcagc ggcacgcttc
     6001 tagttgccgc aaccctgctg acgggcattg ccgggctgct gaaacgcccc ctgacccccg
     6061 catcgctttt cctaatctgc acgcttgccg tcagtatgtg ccacagtatg ctcgaatatc
     6121 ctttatggta tgtctatttc ctcatcccct tcggactgat gctcttcctg tcccccgcag
     6181 aggcttcaga cggcatcgcc ttcaaaaaag ccgccaatct cggcatactg accgcctccg
     6241 ccgccatatt cgcaggattg ctgcacttgg actggacata cacccggctg gttaacgcct
     6301 tttcccccgc cactgacgac agtgccaaaa ccctcaaccg gaaaatcaac gagttgcgct
     6361 atatttccgc aaacagtccg atgctgtcct tttatgccga cttctccctc gtaaacttcg
     6421 ccctgccgga ataccccgaa acccagactt gggcggaaga agcaaccctc aaatcactaa
     6481 aataccgccc ccactccgcc acctaccgca tcgccctcta cctgatgcgg caaggcaaag
     6541 ttgcagaagc aaaacaatgg atgcgggcga cacagtccta ttacccctac ctgatgcccc
     6601 gatacgccga cgaaatccgc aaactgcccg tatgggcacc gctgctaccc gaactgctca
     6661 aagactgcaa agccttcgcc gccgcgcccg gtcatccgga agcaaaaccc tgcaaatgac
     6721 ccgcgccggc ggatgcggat accgcccgaa atgtaaagct ccatgcaaga cattgcaaaa
     6781 aacagcaaac cggtagggaa atacgctatc aaaaaacatt gcagccgtgt taagataaac
     6841 cgtcaaacaa tcttttcacg ccccgcccga aacagggtcg gggcataccc ttacgaaaag
     6901 gaaacaccat gagccgcgta ttactcgtag atgacgatgc cctgctaacc gaactgctga
     6961 ccgaatacct gagcgccgaa ggtctgaacg tccgcagcgt tcccgacggg gaagcaggcg
     7021 tacaggaaat cctgagcggg cagtacgatg tagtcgtatt ggattccatg atgcccaaaa
     7081 tgaacggctt ggatgtcttg aaaaacgtac gcgcccgaag caccgtcccc atcatcatgc
     7141 tgaccgccaa aggcgacgac atcgaccgaa tcatcggctt ggaaatgggc gcggacgact
     7201 atgtccccaa accctgcaca ccacgcgaac tcttggcacg catcaatgcc atcctgcgcc
     7261 gcgcacaaca cagcggcgaa cagaacaacg cacccaacag catctccgtc agcgatgtcg
     7321 tcctgtaccc cgccaaacgc caggcatccg tcaaagacat gccgctcgaa ctgaccagca
     7381 ccgaattcaa cctgctcgaa gtcctgatgc gccatgccgg acaggtagtc agcaaagaaa
     7441 ccctgtccgt cgaagcactc gaccgcaagc tggcaaaatt cgaccgcagt atcgacgtac
     7501 acatctccag catccgccac aagttgggcg atgcctctct gattcaaacc gtacgcggct
     7561 tgggctacct gtttgtcaaa aactgaagca acgggcaaat gaaactgttc caacgcattt
     7621 tcgccacatt ttgcgcagtt atcgtctgtg caatctttgt ggcgagtttt tctttttggc
     7681 tggtgcagaa cacccttgcc gaaaaccagt tcaaccaacg ccgcaccatc gaaaccactt
     7741 tgatgggcag catcatttcc gcattccggg cacgcgggga cgcgggcgcg cgcgaaatcc
     7801 tgacggaatg gaaagacagc cccgtctcat cgggcgtgta cgttatacag ggcgacgaga
     7861 aaaaagatat cctgaaccgg tatatcgaca gctacaccat cgaacgcgcc cggcttttcg
     7921 ccgccggaca cccgcattcc aacctcgtcc atatcgaata cgaccgcttc ggcgaagaat
     7981 acctgttctt caccaaagac tgggacaaac tccaagcccg ccgcctgccc agccccctgt
     8041 tgatccccgg tctgccgctc gccccgattt ggcacgaact catcatattg tccttcatca
     8101 tcgtcgtcgg actgctgatg gcgtacatcc tcgccggcaa tatcgccaaa cccatcagaa
     8161 tcttaggcaa cggcatggac agggtggcaa acggagaact tgaaacccgt atctcccaac
     8221 aggtcgacga ccgcgacgac gaattgtccc atcttgccat ccaattcgac aaaatggtgg
     8281 aaaaacttga aaaactcgtt gccaaagaac gccacctgct ccatcacgtc tcccatgaaa
     8341 tgcgttctcc ccttgcgcgc atgcaggcaa ttgtcggact gattcaggcg cagccccaaa
     8401 aacaggagca atatctcaaa cggctggaag gcgaactgac ccgcatggat acgctggccg
     8461 gggaactgtt aaccctgtcc cgtctcgaaa cttccaatat ggctttggaa aaagaaagcc
     8521 tgaaactcct gcccttcctg ggcaacctgg tagaagacaa tcaaagcatt gcccagaaaa
     8581 acggacaaac ggttaccctg tctgccgacg gaaaaatccc cgaaaataca accatccttg
     8641 ccaacgaaag ctacctgtac cgcgccttcg acaacgtcat ccgcaacgcc gtcaactaca
     8701 gtcccgaagg cagcaccatc ctgatcaaca tcggacaaga ccacaaacac tggataatcg
     8761 acgttaccga caacggcccc ggcgtggacg aaatgcagct cccgcacatc ttcaccgctt
     8821 tctaccgtgc agactccagt gcgaacaaac ccggaacagg actggggctt gcattgaccc
     8881 aacatattat tgaacagcac tgcggaaaaa tcatcgccga aaacatcaaa ccgaacggtc
     8941 tgcggatgcg cttcatcctg cccaagaaaa aaaccggttc caaaacagaa aaaagtgcga
     9001 actgaccata atgccgtctg aaccggcttc agacggcatt gcacaaacag ttaccccaaa
     9061 acaaacacac agcgggtgca tctccaacaa tcacgcacat accgtccgca aaggaatata
     9121 tcatgtcggc acaaaccgat ccgggctact ttttcatgcc caaccacatt attctgatag
     9181 gcgcgagcga acgtccgtac agcctgggcg aacgcgtact cagcaacctg ctgagtacgc
     9241 cctttcaagg aaaaatcacc cccgtaaacc cgcgccacca caccatagcc gggcttcccg
     9301 cctacaccag cctcaacaaa atccccggca atgcagacct gattattgcc gttaccctac
     9361 ccgacagtta cgacaccctc ttcaaaacct gccgcaaaaa gcagctccga cacatcatcc
     9421 tcatacagga ctgggacagc ctgtctgccg cagaactgca caccgccgaa actgccatcc
     9481 gcaaacacca cggcaacgga cttaacatca ccgcctgcac caccgcaggc atccaactgc
     9541 cctcactcgg actcaacatc agtacccaag acggatacgc cgcaggccat accgccatac
     9601 tgaccggcaa tgccgccgtc agccgtcaaa tcgacaacat cctgaacaaa ctccgtcaag
     9661 gcacatcccg ccacatcagc ctgcatcccg gcatcagccc catcacatcc gccgattggc
     9721 tcaaccgctt cggacacagc ctgcacacca aaaccgccgt cctacaccac aaccctgaag
     9781 aagaccagcg caaactgttc agcgcaatcc gccaatttac ccgccatacg ccgctgattc
     9841 tccacatcac ctgccgcacg acagaaaccg accgtgccgt actgcacagc ctcgcccgac
     9901 actgcaactt cctcatcagt ttcaacgccg acgacctcga agccgcactg cgcgcccaac
     9961 tgtccgacct tcctcccctg tcccgactcg acatcctgtc cgacacgcct gccgaatggc
    10021 tgaacgcgca cgcgccaaaa aacctcgccc tccactttcc caaccttccc caccacatcc
    10081 gcaacggaca cctgaccggc acacccacac cctcaatctg ccacgacatc gcctcacgtc
    10141 agcttgcccg ccccgacacc caagccgtcc taaccatcct cagtccctcc ggacacgagg
    10201 attacaaaaa aacagcacgc gcccttatcc gcctgtccga acagaccgcc aaacccctgc
    10261 tcgtcagcag ccccttttca gacggcataa cacatttcga cacccccact caggcaatcc
    10321 gcaccctttc ctaccgcaac accgccgccg ccctgaaaca ggcacagctc gacattgcac
    10381 cgccgcagcc atgccgtctg aaaacaccgc aaccccaaaa catcaaaaaa gcccttgcag
    10441 cggcaaaccc ctccctgctc gccgaagccc tgcacctccc cccctaccgg cacaccaccc
    10501 ataacgccgt acaattccaa ttcgacagcc accccctcta tggcgacatc ctgaccgcac
    10561 gctgcaacgg acaaaccact gccgtactcc cgccgtttac cacgctcgac agccgccacc
    10621 ttgcccgctt tgccgaactc gacggcacac aaaccctcga ccagttcctg cacacactga
    10681 ccatcattcc cgaataccgc caacacattc tcggcatcac cctcaaccta aacggcggac
    10741 aatacagcag cgatttcatc ttaaagacac ccgaaacaca cgacacgccc aaacgcaaga
    10801 acacaggcaa agccgcccaa accctcgaac atgccgccgc aaaaatgcag agtgccgccg
    10861 catacctgaa acacaaaaac ccgacagccg ccgaatttct ccgccacaca agcgaagccg
    10921 ccgcagaact gctcggcagc aaaaccgaaa ccggagcagc cgtacccaac gtacttgccc
    10981 cctatcccgc agcatacccc aaaacactgt ccctaagaaa caacacgacc gttaccatta
    11041 cccccatttt gcccgaagac gcagaagcca aacagcagtt cgtccgcagc ctcggtcccg
    11101 aagcacggta cacacgcttc atgacccaca ccaacgaact gcccgcagcc acgttggcac
    11161 gcctgtgcaa ccccgattac cactgtgaag ccgcatggac ggcaaaggat gccgacagca
    11221 acatcgtcgc cgtcgtccgc cacagccgcc tgaatcgcaa cgaatgcgaa ttcggcatca
    11281 cactggcgga acatatgcgc ggcagcggac tggcacagaa aatgatggaa ctcatcatcc
    11341 aaaccgccgc acagcaaggc taccggacta tgagtgccga cattctcaaa accaataccc
    11401 ccatgatcaa acttgctgaa aaatcaggat ttaccctcaa ggaatcggac accgaaaaaa
    11461 acctgtaccg cgcatatctg aaccttgcgg cagacaaaac aacagaaaaa acaaataaaa
    11521 acttgcgcac cgaccacaaa ataacctaaa atcggcagtt tccatatatc cgattttctc
    11581 aaaaggactc aaaatggtag ttatccgttt ggcacgcggc ggctcgaaac accgcccctt
    11641 ctacaacgtc atcgttactg actcacgcag ccgccgcgac ggccgcttca tcgaacgcgt
    11701 aggcttctac aaccccgtag ccaacgaaaa acaagagcgc gtccgcctca atgcagaccg
    11761 cctgaaccac tggattgcac aaggcgcgca agtcagcgac tccgttgcaa aactgattaa
    11821 agaacaaaaa gccgcctaat ccgcatttgc cgccatgaca gacactcaaa accgggtagc
    11881 catgggctac atcaaaggcg tattcggcat aaaaggctgg ttgaaaattg ccgccaacac
    11941 cgaatattcc gacagccttt tggactaccc cgagtggcat ttggtcaagg acggcaaaac
    12001 catcagcgtt acccttgaag ccggaaaagt cgtcaacggc gaactccaag tcaaattcga
    12061 aggcataaac gaccgcgact tggcattctc attgcgcggt tacaccatcg aaataccccg
    12121 tgaagcattc gccccgacag aagaagacga atactactgg acagacttgg tcggcatgac
    12181 cgtcgtcaac aaagaccata ccgttttagg caaggtaagc aacctgatgg aaaccggcgc
    12241 aaacgacgta ttgatgattg acggagaaca cgggcagatt ctgattccgt tcgtttccca
    12301 atatatcgaa accgtcgata ccggcagcaa gattattact gccgactggg gtttggacta
    12361 ctgatgctta tccaggcagt taccattttc cccgaaatgt tcgacagcat tacccgctac
    12421 ggcgtaacgg gacgcgcgaa cagacaggga atctggcagt ttgaagcagt caatccccga
    12481 aagtttgccg acaacagatt gggctacatc gacgaccgcc cgttcggcgg cggcccggga
    12541 atgattatga tggctccgcc gcttcatgca gcaatagaac acgccaaagc acaatcttcc
    12601 caagctgcaa aagtcattta cctcagcccc caaggaaaac cgctgacaca cccaaaagcg
    12661 gcagaactgg cagaacttcc gcatctgatt ctgctgtgcg gacgctatga gggaatagac
    12721 gaaagactgc tgcaaaccag cgtcgatgaa gaaatcagca tcggagactt cgttgtttcc
    12781 ggcggagagc ttcccgccat gatgctgatg gatgcggtat tgaggctcgt acccggcgta
    12841 ttgggcgata tgcagtctgc cgaacaagat tcgttctcaa gcggcatttt ggactgcccc
    12901 cactacacca aacccttaga atttcaaggc atggctgttc cggaagtatt acgttccggc
    12961 aatcatggct tgatagcgga atggcggttg gaacaatcgc tgcgccgcac cttggagcgc
    13021 agacccgatc ttttggaaaa gcgcgtttta atcccaaagg aatcccgcct cttagaaacc
    13081 atccggcaag agcaacggga aatccaatca taatttagga aaaaaacaat gaacctgatt
    13141 caacagctcg agcaagaaga aattgcccgc ctgaataaag aaatccccga attcgcaccg
    13201 ggcgacaccg tagtcgtatc cgtacgcgtc gtggaaggta cccgcagccg tctgcaagcc
    13261 tacgaaggcg tggttattgc ccgtcgcaac cgtggtctga acagcaactt catcgtccgc
    13321 aaaatctcca gcggcgaagg tgttgaacgt actttccaac tgtactctcc gaccgtcgaa
    13381 aaaatcgaag tcaaacgccg tggcgacgta cgccgtgcca aactgtacta cctgcgcggc
    13441 ctgaccggta aagctgcacg catcaaagaa aaactgcctg cacgcaaagg ttgattcaaa
    13501 ccgttttccc caatgccgtc tgaaccttct gacggcattt cttattgccg caacatccgc
    13561 aacggaacag caccaagaag cacacccctt ccggcacatc ccgaatcccc gtccgaaccg
    13621 aacaaaattt acagaccaaa cgttatacag tatcatatca gccatataat aataaccgct
    13681 ttaccgcttt ccctttcaaa accgcaaatc atcatgacac cttccctttt actatcagga
    13741 ttgaccttcc gcctcgtcct tgccctgatt gccgtatccc ttttatgggg cgtttacttt
    13801 tgggcagtat ccgcatgagc atcattgtcg aaaacctgac cgtcagctac cgccgccgtc
    13861 ctgccgtaca ccacgttgac attacttttg aagaacatag tatgtgggcg gttttcggtc
    13921 ccaacggcgc agggaaatcc acctttctca aatccttgat gggattgcag cctatcgata
    13981 caggcagcat ccggctggat ggattgagcc gccaagacat cgcctacctt ccccagcagt
    14041 ccgatatcga ccgctcccaa cctatgactg ttttcgactt ggcggcaatg gggctttggt
    14101 atgaaatcgg ctttttcaaa gggataaata ccgctcaaaa acaacgcgtt cacgaagcct
    14161 tggagcgtgt aggaatgcgg cagtttgcac gccgccaaat cgcccacctc tcaaacggac
    14221 aattccaacg cgtccttttt gcccgaatgc tggttcaaaa tgcaaaattc ctgcttttgg
    14281 acgaaccttt caacgccgtt gatgcacgga cgacctacga acttctcgac gtattgcaga
    14341 aatgccattg cggcggacac gctatcattg ccgtactgca cgattacgaa caagtccgtg
    14401 cctactttcc cagtaccctg ctgctcgccc gagaaaaaat tgcggcaggc gcaaccgaga
    14461 ccattctgac agaaccctac ctcgcccaag ccaacgccaa aatgcagcaa caggaaagcc
    14521 ccgactggtg cgcctcataa atactgcccg aaaccacgcc ccacaccaga caccatgaat
    14581 ctctacgacc tgctccttgc cccctttgca gaattcgact ttatgcgcta cgccctcgca
    14641 tccgtcttct gcctgtccct cagtgccgca cccgtcggcg tattcctcgt catgcgccgt
    14701 atgagcctga taggcgacgc attgagccac gccgtcctgc ccggtgccgc cgtcggctac
    14761 atgtttgccg gcttgagcct gcccgccatg ggtttgggcg gcgtagccgc aggcatgctg
    14821 atggcactgc ttgccggact cgtcagccgc ttcaccaccc tgaaagaaga tgccaacttt
    14881 gctgcctttt atctcagcag cctcgccatc ggcgtagtcc tcgtcagcaa aaacgggagc
    14941 agcgtcgatt tgctccacct ccttttcggc tctgtacttg ccgtcgatat tcctgccctg
    15001 cagctcatcg ccgccgtctc cagcctcacg ctcattaccc ttgccgtcat ctaccgcccg
    15061 ctcgtactcg aaagcatcga ccccctgttt ctcaaatccg tcggcggcaa aggcgggctt
    15121 tggcacgtcc tctttctcgt cctggtcgtc atgaacctcg tatccggctt tcaagccctc
    15181 ggcacactca tgtccgtcgg actcatgatg ctgccagcca ttaccgcccg cctgtgggcg
    15241 aagcacatgg gcgcactcat cctcctatcc gttctgacag ccctgctgtg cggcttgagc
    15301 ggactgctca tttcctacca catcgaaatt ccctccggtc ccgccatcat cctctgttgc
    15361 agcgtccttt atctcttttc cgtcatactc ggcaaagaag gcggcattct gaccaaatgg
    15421 ctcaaaaacc accgccacca caccacctga ccccgcaagc cgcttgaaca aacaggcagc
    15481 ttgttttacc ataacagatg ttacgttata tcacaggaga acagttatga aacacctcaa
    15541 actcaccctt attgccgcat tgctaaccac cgccgcaact gccgcccccc tgcccgtcgt
    15601 aaccagcttc agcattttag gcgacgttgc caagcaaatc ggcggagagc gcgtatccat
    15661 acaaagtttg gtcggagcca accaagatac tcatgcctat cacatgacca gtagcgacat
    15721 taaaaaaatc cgcagtgcaa aactcgtcct gctcaacggc ttaggacttg aagctgccga
    15781 tgtacaacgt gccgtcaaac agagcaaagt atcctatgcc gaagcgacca aaggcatcca
    15841 acccctcaaa gccgaagaag aaggcggaca ccatcatgac cacgaccatg accacgaagg
    15901 acaccaccat gaccacggcg aatttgaccc gcacgtctgg aacgaccccg tccttatgtc
    15961 cgcctatgcc caaaacgtcg ccgaagccct gataaaggcc gaccccgaag gcaaagttta
    16021 ttatcaacaa cgcttgggca actaccaaat gcagcttaaa aaactgcaca gcgacgcaca
    16081 agccgcattt aacgccgtcc ctgccgccaa acgcaaagtc ctgaccgggc acgatgcctt
    16141 ttcctacatg ggcaaacgtt accatattga attcatcgcc ccgcaaggtg tgagcagcga
    16201 agccgagcct tcagccaaac aagtcgccgc catcatccga caaatcaaac gcgaaggcat
    16261 caaagccgtc tttaccgaaa acatcaagga cacccgtatg gttgaccgta tcgccaaaga
    16321 aaccggtgtc aacgtcagcg gcaaactgta ttccgacgca ctcggcaacg cgcccgcaga
    16381 cacctacatc ggcatgtacc gccacaacat caaagccttg atcaacgcga tgaagcaata
    16441 aaaccgtttg attggtttga atttgaaaag gcaaaaggcc gtctgaaaat tatagtggat
    16501 taacaaaaat caggacaagg cgacgaagcc gcagacagta cagatagtac ggtaaggcga
    16561 ggcaacgctg tactggtttt tgtgaatcca ctatattgct agaagtgcac cgattaaggt
    16621 taattaaaat acgaccctta tcccaatatt aagaaaaaca gcaatcaaaa taaacttgat
    16681 tgctgttttt cagtttaagc catgaatgtg attaggctcc ttatgcagat ggcgttacca
    16741 ataacggctg ctgtacggat tgtgtattgg catccacatt tcctcctgtc gcagcagtat
    16801 tagaaccgaa cacagacata gactgtacca aattattgcc agcattaata tacttggcaa
    16861 aatctggatt actgatagct gcatcatcaa agacaaatgt atctacacga tggttttcac
    16921 catagaagaa tccggaaata cgtacgttgt cttgttcaga agcgctaagc accaaatcac
    16981 ttccggaacg gataaaatga acatctgctg ctttaaatcc tttaaagtgc atagtgtcag
    17041 agtttttatc cacatggtaa ttatagaccg tatcctgacc gaagcctttg ccgaagacat
    17101 aagtatccga accgctgccg ccctccaagt aatcattgcc tgcaccgccg attagagtgt
    17161 cgttaccgtc ttcgccgttc aaatgatcat tgccttcgcc accattcagt acatcgttac
    17221 cattatagcc gtacagggcg tcgttgcctt ctcctccatt gagcgtatca ttgccattgc
    17281 cactgtagat actgtcgttg cctgcatcac cattcagcag gtcatccccg tcggcaccgt
    17341 acagatagtc atcgcccaat ccgccattta aggtatttcc ggattggtag gcatacaatc
    17401 tgtccgaacc gtcggtggat tgctgtacca gtt
//
LOCUS       DAXHTR010000028        17176 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.028, whole genome shotgun sequence.
ACCESSION   DAXHTR010000028 DAXHTR010000000
VERSION     DAXHTR010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17176
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.028"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..101
                     /locus_tag="ACFNML_000665"
     CDS             <1..101
                     /locus_tag="ACFNML_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HFC6392081.1"
                     /translation="TFACVLGALVASEHGATPDISPERIERFMNPV"
     gene            complement(161..718)
                     /locus_tag="ACFNML_000666"
     CDS             complement(161..718)
                     /locus_tag="ACFNML_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6392082.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFQAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(794..2428)
                     /locus_tag="ACFNML_000667"
     CDS             complement(794..2428)
                     /locus_tag="ACFNML_000667"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFC6392083.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(2706..3812)
                     /gene="serC"
                     /locus_tag="ACFNML_000668"
     CDS             complement(2706..3812)
                     /gene="serC"
                     /locus_tag="ACFNML_000668"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HFC6392084.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGATVVIIRED
                     LLERCPNDIPDVFNYRSHINRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFQTEDEELDRRFVLEAELQGLCLLKGY
                     KTVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4177..4608
                     /gene="rimP"
                     /locus_tag="ACFNML_000669"
     CDS             4177..4608
                     /gene="rimP"
                     /locus_tag="ACFNML_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFC6392085.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKEGGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIELGNIDKARLRPEFKF"
     gene            4636..6138
                     /gene="nusA"
                     /locus_tag="ACFNML_000670"
     CDS             4636..6138
                     /gene="nusA"
                     /locus_tag="ACFNML_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFC6392086.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTTIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGVDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6151..9039
                     /gene="infB"
                     /locus_tag="ACFNML_000671"
     CDS             6151..9039
                     /gene="infB"
                     /locus_tag="ACFNML_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFC6392087.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDTAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKTAQEAKTGRQRPAKPAEKPQAAAPAVENKPVNPAKAKKEDRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGSKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRTKVVQGEAGGITQHIGAYHVET
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPM
                     VVAVNKIDKEAANPERIRQELTAHEVVPDEWGGDVQFIDVSAKKGLNIDALLEAVLLE
                     AEVLELTAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQVTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHVRLIRNNVVIHTGE
                     LASLKRYKDDVKEVRMGFECGLMLKGYNEIMEGDQLECFDIVEVARTL"
     gene            complement(9250..10644)
                     /locus_tag="ACFNML_000672"
     CDS             complement(9250..10644)
                     /locus_tag="ACFNML_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HFC6392088.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVRNAPSTTRHV
                     EEAAISDGADTLVFLYDTPGLEDAGGVLEWLENHTDNRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQPPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLIDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(10718..12058)
                     /locus_tag="ACFNML_000673"
     CDS             complement(10718..12058)
                     /locus_tag="ACFNML_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HFC6392089.1"
                     /translation="MLNPSRKLVELIRILDEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRETLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLSNAASVRAVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIACYGILPRLLAWAVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12201..12827
                     /locus_tag="ACFNML_000674"
     CDS             12201..12827
                     /locus_tag="ACFNML_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFC6392090.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLALMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIAIYVVMGWMVLAVMKSLTASLPPAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(12891..14309)
                     /locus_tag="ACFNML_000675"
     CDS             complement(12891..14309)
                     /locus_tag="ACFNML_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6392091.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAVRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYQYEFHEFHDKG"
     gene            14636..15364
                     /locus_tag="ACFNML_000676"
     CDS             14636..15364
                     /locus_tag="ACFNML_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFC6392092.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     SNPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(15423..15911)
                     /locus_tag="ACFNML_000677"
     CDS             complement(15423..15911)
                     /locus_tag="ACFNML_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HFC6392093.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGAVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWSLFDWFEPVVRGFGNCAEPDYLLGVALPVWSAAYFLAVVLTVWWAWAR
                     AK"
     gene            15941..16153
                     /locus_tag="ACFNML_000678"
     CDS             15941..16153
                     /locus_tag="ACFNML_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFC6392094.1"
                     /translation="MPILLDISRDGKGRIDTGSGADDFGFPRYAQNLTRDKTAICPHN
                     AHKKIHPEDLNFSKFSESKVYFNEII"
     gene            complement(16670..17134)
                     /locus_tag="ACFNML_000679"
     CDS             complement(16670..17134)
                     /locus_tag="ACFNML_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HFC6392095.1"
                     /translation="MKELDKIDFRILKILQQNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKSGNIFEDFKKEVLKIPQIMECHLVS
                     GEYDYLIKVRLPDMSAYRDMLGNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4504 a         4784 c         4338 g         3550 t
ORIGIN      
        1 agacctttgc ctgcgtactt ggtgcgctgg tcgcctccga acatggcgcg acacccgaca
       61 tttcccccga acgcatcgag cgtttcatga atccggttta aaacgcatgg aaaaatgccg
      121 tctgaaagcc tttcagacgg catttcgctt gagattccgt ttaccaatgg ctgacaaacg
      181 cttccaaatc ggtattcttg ggcttatgca cttcctctgt cggcgtgccg accatcatca
      241 gcccgatgat tttatcctta tccgcacatc cgaaagcctc acgcaacagg gggctgttga
      301 cccacatccc cgtaatccag acattgtcga aaccttgcgc cgttgccgcc agttgcagcg
      361 catacgccgc acaacccgcc gtcagcatct gctcccattc cggtttcggc ttaggcacat
      421 cgcggttcgg cgcaaacgtt acaccaatga ccatcggcgc catattgccc actttttccg
      481 cctttttcat cgcatcgtcg ccgaaattca attcggcaac cgtttgcttc aacacatcgc
      541 gaaaacgttg caatcctacc tcgccttgaa tcacggtaaa acggaagggg cgcatattgc
      601 cgtgatcggg aacttgggtt gccgcctgaa atatttgttc caactccgcc gcatcggggg
      661 cggggtgctt cagctttttg gaagatcggc ggttcgtcaa taattttaaa gcatccatat
      721 cgttatactc cggtcatcgg tcgggtgttc ggacacatat gccgtccgaa ggcttcagac
      781 ggcatatccg gcatcagcgc ggacggcggc aggctgccaa tatatccatt tccttccgat
      841 aggtttggct attggaaatg tccatcagcc ccaataccgt gctgaaatag tggtcgtgcg
      901 aatattcgtt ttccgccgct ttttgtttga ggcattggaa atctatgccc ccgtgttggc
      961 ggaaggcttt ggaaaaccac ataaccatcg ggatatgcgt ctgcccggaa ggcgcgatgg
     1021 cgtaaggcgc ggcgtgcagg tacatcccgt tttcgcccaa actttcgccg tggtcggaaa
     1081 cataatgcac cacgctttcc aaatcgtcgc ggttttcaag tttgcggata accttgtcga
     1141 taaactggtc cacatacaaa accgtattgt cgtaagtgtt gaccagcgtg gcgcgggtgc
     1201 atttgttgat ttcgttggtg tcgcaggtcg gcgtgaattt gcgttcggct tcggtatagc
     1261 gttcgtaata cgtcggcccg tggctgccga tggtatgcag gattaaaacc gcgtctttat
     1321 cgtttttgtt gaggacttcg tcgaacttag tcagcaggat attgtcgagg cactcgccgt
     1381 tgcggcagta ttcgggcagg ttgagcgagg taacgtcggt attcggcact ttgccgcaca
     1441 cgcccttgca gccggaatcg ttttccaacc aggtaacttc cacgccggcg cgctgcacga
     1501 tgtccagcag gttgtcttgg tgttcggctt tgatttcgtc ataatccgtg cggtcgaagg
     1561 ttgagaacat acacggcagg gagtgcgcgg tcgatgtacc gcagcttctg acctgcggga
     1621 aattgacaat ttcatcgccg cgcgcggcaa gcagcggcgt agtttggcgg ctgtaaccgt
     1681 tcaaacccca gttggcggca cgcgtggtct cgcccacgac cagcaccacg aaacggcgca
     1741 ggctgccggc cggccggttt tgcacgaccg ccatatccaa ttgcgtataa ggaatattgg
     1801 aacgcttcca atctttgtat ttcgacacgc ccgcgccgat gaaattagac ggcacaatca
     1861 gatgggttac tgatttattg ttgcggaaaa acgaggcgta atcctgatat tgcaacattg
     1921 cgatgcccaa cgcgcacaaa aaggaaacgg cggcaagcac aaggcgcgtc aaaagctcct
     1981 tataccaaac gcggtattta accttgacgg cgatatacgc cagcgcgggc aatacgccca
     2041 aacatacaat ccacagcaca tagcccggcg taatcaggcg cgcgctttcg gcagccgtag
     2101 tttgcaagac attattcaac atcgacttgt tgaaatagat attgaaaaat atttcttggt
     2161 aagacaccgc cgcactgata accaatatca acggaatcaa taccttatgc acgaaaggca
     2221 gggcaatgac gtgaaaaacg aaattactta aaaaaaacag caccaccggc atcgtataga
     2281 ggaagatatc cgccccggtg ccgttaaaag gatgaagctc gacaactttg gcaaaaaagg
     2341 cgtaattcaa taccagcgag gaatacaggg aaaggaaggc aatcagcgcg gaagagccga
     2401 gcttcggcct caggttcggt tttatcattt ggaatgtgtc ggataagggt tggaaaaggc
     2461 atccggcatt tggaatccgg attattgaaa aagattctta attataaggc aacggagcaa
     2521 agcagggcaa gaaaacggaa ggcgacgcag gctgtcggat acacatctcg gcacaaacag
     2581 catcacgccc ggattgaaaa ggacggatag ccgccggcag cacaatctta ccacctccaa
     2641 aacgccgccg gagaacgccg aaacagccga tgccgtctga agccgcttca gacaacatcg
     2701 ggacatcaac cgtaacgccg ttggaaatcg cgcataaaat ctgccaaagc ccgcacgcct
     2761 tcaagcggca tcgcattata aatgctggca cgcataccgc cgacggtttt atagccctta
     2821 agcaggcaca agccctgcaa ttcggcttcc agcacaaaac ggcggtcaag ctcctcatcc
     2881 tccgtttgga acacgacatt cattttagaa cgcgcattcg gacggatacg gttgatatag
     2941 aaaccgccgc tgccatctat cgtctcatac aaggtttgcg ccttcagccg attgaccgct
     3001 tcaatttttt tcacaccgcc ctgcgcctgc agccagcgga acaccagccc cgacatataa
     3061 atcgcgtaag ttgacggcgt gttgtacata ccgtcgcggt tgatgtgcga acggtagttg
     3121 aacacatcgg gaatatcgtt cggacaacgc tcgagcaaat cctcgcggat aatgaccacg
     3181 gtcgcaccgg ccgggccgat gtttttctgt gcgcctgcgt aaatcagtcc gtagtcggca
     3241 acatcaaact cgcgcgacaa aatctcgctg gacatatcgc acaccagcgg cggcatgcct
     3301 tctgaaaggc gcggcacttc acggtattgc agcccgttga ccgtttcatt gacggcaaaa
     3361 tggacaaacg ccgaatcggg tgcaacatcc cacgtttcca caggcggcag gtcgagatag
     3421 tcgaactgct cgccgccatg cgccgccaaa cggatttccg tatcggtcaa acggctcatc
     3481 tgttcataag cgatacggct ccagttgccc gttaccaccg cgtcggcagt gcggaaaccg
     3541 tgtgccagat tcatggctgc catattaaat tgggttgttg ctccgccctg cagaaacaat
     3601 atcttatagt tgtcaggcac tttcaaaagc tgcctcaaat cctgttccgc atgatgcagg
     3661 atgctcaaaa acatttccga acggtggctc atcgccatca cgggaaaacc cgtaccgttg
     3721 tagtccaaca tttcctgccg cgccgtttcc aacacggctt cgggcaatac ggcagggccg
     3781 gcggaaaaat tgtaaatcgg ataaagagac atgatgcagc cttgattctg aacaataacc
     3841 cgcctgattt taggcttgca gcaggctttg tgcaaggatg ggaaatacct gtcctccgcc
     3901 ccgcttcatg ccgcccgaac acggaaaaca acatcaatat attgatttac aaatataaaa
     3961 atcatgctag cgacaaatag atacatttgt tttgtcaaca atattcacga tttcccatta
     4021 caaacctccc ttacacccgc ttttttccgt cccaaaaaca caaaataaat caacactttc
     4081 atttctccgc aaaagcggtt ataatcacgc cgatttttca actttgacga aaaatggcca
     4141 cggggccatt tttttattat gtacataggg agcagcatgg atatccaaac catcctcgaa
     4201 aaaaccctgc ccggcctggg ctacgaactg gtcgatttcg aactgaccgc gcaaggaaca
     4261 ttgcgcgtgt tcatcgacaa agaaggcggc attaccgtcg aagactgcgc aaccgtcagc
     4321 aaccacttga gccgcgtatt tatggttgaa gacatcgact acaaaaacct ggaaatttcc
     4381 agccccggac tcgaccgccc cttgaaaaaa gccgccgact tcgtgcgctt tgccggtcag
     4441 aatgccaaaa tcaaaacccg cctgccgata gacggtcaaa aaaactttat cggtaaaatc
     4501 gaaggctgcg aaaacgacac tgttaccgtt tccttcgacg gcaaaaccgt acaaatcgaa
     4561 ttgggcaaca tcgacaaagc ccgcctgcgc cccgaattca aattctaaaa cacaacaata
     4621 ttggagatgt tcaaaatgag tcgtgaaatg ttacagctgg cagaagcact ggcaagcgaa
     4681 aaaaacgttg atgccgaagt cgtcttccaa gcactggaat tcgccctgtc taccgccgcc
     4741 aagaaaaagg cagaccgcga acacatggat gtccgcgtac aaatcaaccg cgacaccggc
     4801 gaataccaaa ccttccgccg ctggctgatt gtcgccgatg aagactatac ctatcccgat
     4861 gtcgaaaaaa ccatcgagga aatccaagag gaaattcccg gcactaccat ccaaatcggc
     4921 gaatactacg aagagcagct gcccaacgaa ggcttcggcc gccaagccgc gcaaaccgcc
     4981 aaacaaatca tcctgcaacg catccgcgat gccgagcgcg agcagaatct gaacgagttt
     5041 ctcgccgtca aagaagacat cgtgtccggc acggtcaaac gcgtcgaacg ccacggcatc
     5101 atcgtcgaag tcgttgccgg caaactggac gcgctgattc cgcgcgacca aatgattccg
     5161 cgcgaaaact tccgcagcgg cgaccgcatc cgcgccctct tcctgcgcgt cgaagaaatc
     5221 ggcaacaccg gccgcaaaca agtcatcctg agccgtactt ccggcgattt cctcgtcaaa
     5281 ctgtacgcca atgaagtacc tgaaattgca gacggcatgc ttgaaatccg cgctgtcgcc
     5341 cgcgacccgg gccagcgtgc caaagtcgcc gtcaaagcca acgaccagcg catcgatccg
     5401 caaggcacct gtatcggcgt tcgcggttcg cgtgtcaatg ccgtcagcaa cgaattgtcc
     5461 ggcgagcgca tcgacgttgt cctttggtcg cccgagcctg cccaattcgt catgagcgcg
     5521 ctctcacccg ccgaagtcag ccgcatcgtc atcgacgaag acaaacacgc cgtcgatgtc
     5581 atcgttgccg aagaccagct cgcgctcgcc atcggacgcg gcggtcaaaa cgtgcgcctt
     5641 gcttccgacc tgaccggctg gcagctcaac atcatgactt ccgccgaggc agacgaacgc
     5701 aatgcggcag aagatgccgc catccgccgc ctgtttatgg atcacttgaa cgtggacgaa
     5761 gaaaccgccg acgtactggt tcaggaaggt tttgcaacct tggaagaagt cgcctatgtt
     5821 cctgccgccg aactgcttgc cattgaagga tttgacgaag aaatcgtcga tatgctccgc
     5881 aaccgcgccc gcgatgccat cctgaccatg gcgattgccg ccgaagaaaa actgggcgaa
     5941 gtatccgacg atatgcgcaa cctcgaaggc gtagatgccg atatgctccg cagccttgcc
     6001 gaagcaggca ttaccacccg cgacgacttg gcagagcttg ccgtggacga actgattgaa
     6061 attaccggtg taaacgaaga aaccgcaaaa gccgtcatcc tgaccgctcg cgaacactgg
     6121 tttaccgaag acaaataaaa gggggtacag atgagtaaca caaccgtaga acaatttgcc
     6181 gccgagctga aacgccccgt cgaagacctg ttgaaacagt tgaaagaagc cggcgtcagc
     6241 aaaaacagcg gcagcgattc cctgacgctg gacgacaaac agcttctgaa cgcctacctg
     6301 accaagaaaa acggcagcaa cggcggcacc atcagcatcc gccgcaccaa aaccgaagtc
     6361 agcaccgttg acggcgtaaa agtcgaaaca cgcaaacgcg gacgcactgt caacattcct
     6421 tctgccgaag aattggcagc acaggtaaaa gccgcccaaa cccaagccgc acctgtccgg
     6481 ccggagcaga cggcagaaga cacggcaaaa gcccgagccg aagctgccgc acgtgcagaa
     6541 gcccgtgcca aggcagaagc ggaagcggca aaactgaaag cggcaaaagc aggcaacaaa
     6601 gccaaacctg ccgcgcagaa acccaccgaa gcaaaagccg aaaccgcacc tgttgcggcg
     6661 gaaaccaaac ccgccgaaga aagcaaagcg gaaaaagccc aagccgacaa aatgccgtct
     6721 aaaaaacccg ccgagcccaa agaaaaagcc gccaagccga aacacgagcg aaacggcaaa
     6781 ggcaaagatg ccaaaaaacc agcgaaacct gccgcacctg ccgtgccgca acccgtggtc
     6841 agcgcagaag aacaggcgca acgcgatgaa gaagcacgcc gtgccgccgc acttcgcgcc
     6901 caccaggaag ccctgttgaa agagaaacag gaacgccagg cacgccgcga agccatgaaa
     6961 caacaggcag aacaacaggc aaaaaccgca caggaagcca aaaccggcag acagcgtccc
     7021 gccaaacctg ccgagaaacc gcaggcagcc gcgccggccg tcgaaaacaa gcctgtcaat
     7081 ccggcaaaag cgaaaaaaga agaccgccgc aaccgcgatg acgaaggtca aggccgcaac
     7141 gccaaaggca aaggcggaaa aggcgggcgc gaccgcaaca atgcacgcaa cggcgacgac
     7201 gagcgcgtac gcggcagcaa aaaaggcaaa aaactcaaac tcgagccgaa tcaacacgcc
     7261 ttccaagcac cgaccgaacc cgtcgttcat gaagttttgg ttcccgaaac cattaccgtt
     7321 gccgatttgg cgcacaaaat ggcggtcaaa ggcgtggaag tggtcaaagc cctgatgaag
     7381 atgggcatga tggttaccat caaccaatcc atcgaccaag acaccgccct gattgtggtg
     7441 gaagaactcg gccacatcgg caaacctgcc gcagccgacg accctgaagc attcttggac
     7501 gagggcgcgg aagcggtgga agccgaagca ttgccgcgtc cgcccgtcgt taccgtcatg
     7561 ggccacgtcg accacggcaa aacctcgctg ctggactaca tccgccgtac caaagtggta
     7621 cagggcgaag caggcggcat tacccaacac atcggtgcat accacgttga aaccccacgc
     7681 ggcgtgatta ccttcttgga tactccgggt cacgaagcgt ttactgccat gcgtgcgcgc
     7741 ggtgcgaaag caaccgatat cgtgattctt gtggtcgccg ccgacgacgg tgtgatgccg
     7801 caaaccatcg aagcgattgc ccacgccaaa gcggcaggcg taccgatggt ggttgccgtc
     7861 aacaaaatcg ataaagaagc cgccaaccca gagcgtatcc gccaagagct gaccgcacac
     7921 gaagtcgtgc ctgacgaatg gggcggcgat gtacagttta tcgacgtttc cgctaaaaaa
     7981 ggcctgaaca tcgatgcatt gctcgaagca gtcttgctcg aagccgaagt tttggaactg
     8041 actgcccctg tcgatgcacc tgccaaaggt atcatcgttg aggcacgttt ggacaaaggc
     8101 cgcggcgcgg ttgccacatt gctggttcaa agcggtacgc tgaaaaaagg cgatatgctg
     8161 ctggccggta cggcattcgg caaaatccgc gcgatggtcg atgaaaacgg caaatccatt
     8221 accgaagccg gcccgtccat ccccgtcgaa atcctcggct tgtccgacgt accgaatgcg
     8281 ggtgaagacg cgatggtatt ggcggacgag aaaaaagcgc gcgaaatcgc cctcttccgc
     8341 caaggcaaat accgcgacgt gcgccttgcc aaacagcagg cggcgaagct ggaaaatatg
     8401 ttcaacaata tgggcgaaac ccaggcccaa tctttgtcgg tcatcatcaa ggcagacgtt
     8461 cagggttctt acgaggcttt ggcgggcagc ctgaaaaaac tgtccaccga cgaggtgaaa
     8521 gtgaacgtat tacacagcgg cgtgggcggc attaccgaat cagatgtcaa ccttgccatc
     8581 gcttcgggcg cgttcattat cggctttaac gtgcgtgcag atgcctcttc gcgcaaactt
     8641 gccgaaaatg aaaacgtgga aatccgctac tacaacatca tctacgatgc catcgacgac
     8701 gtgaaggcgg cgatgagcgg catgctttct ccggaagaga aagagcaagt aaccggtacg
     8761 gtcgaaatcc gtcaggtcat ctccgtttcc aaggtcggca acattgcagg ctgtatggtt
     8821 accgacggcg tggtcaaacg cgattcccat gtccgcctca tccgcaacaa cgtggtcatc
     8881 cacacgggcg aactggcttc gttgaaacgc tataaagacg acgtcaaaga agtccgtatg
     8941 ggctttgagt gcggcctgat gctcaaaggc tacaacgaaa tcatggaagg cgaccaactg
     9001 gaatgcttcg acatcgtcga agtggcacgc accctgtgat tttgatgcgg aaaaatgccg
     9061 tctgaagcgt tcagacggca tacggaacgg gttctgtatc atacagaacc cgttttttat
     9121 cgcaaatcgg cttcagacag ccctcctgcc ttatcccgat ttgaatctga cttgccatac
     9181 aaacaggctt cagacggcat tatagtgaat taaatttaaa ccggtacagc gttggctcgc
     9241 cttgccgtac tatttgtact gtctgcggct cgccgccttg tcctgattta aatttaattc
     9301 actatatttt cccgacaggc ggatttgaag tttctccaca tatccggcgc gttcggcgcg
     9361 gctggtttcg gggcggtgcg tattgagcga cgaccatttc caatgactgc gggctttgtt
     9421 gagttcgggc gggagtctgg cggcgtccca cggggctttg cggctgtgca gctcgatatc
     9481 cgactgtgcc gcgtgtccgc gcgtttgcag gacgtggagc aaatcgaggg cgcgggcggc
     9541 gagcagggtc aaggtttcag ggtcggtgtg caaggtttgg cggccggaga gtttgtcgga
     9601 aatggtgcgg gtattgggca ggatgccgcc caaaaagccg ccgattgccg tacccaatcc
     9661 gagcgagccg ccgagtgtgg cgatgtccag ccccaagccg atgagcgcgc cggttgccgc
     9721 gcccgtgccg gtgcggatgc cgtattgttt gagcaattcg ctgtcgaacg ggtcttggcg
     9781 gaaggcttgc ggcatccagt cgccgccgtc gatttcgctg tggtagaaac ggtagagggc
     9841 aaacagccgc tgctgcatct gccgttcgag ttggcgtatt tccgcctgca tggtttgtag
     9901 cacggtggcg gtatcctcgt tttcgtccac ttcctgcctg aaggcggcgg catcaattaa
     9961 aaagtcggcg atttcgcggc gcgcttcgcc gtccagccgc tgccattcgc gccggcgcat
    10021 ggctgtcagg cggtcaagtg tgctgcgttc gggcaacatg gtggcgaggt tttcccacag
    10081 gcgtagttcg ccttcaaaat caaaggcgac ggtgtcgaac cctgcgaaaa cgtgcaggtt
    10141 tctcctcgcc agcatggttg tccacgattc gggaggctgt ccgccggtaa agttgaacac
    10201 gggcataacc ggtttggcac accatgaaag gatggtcaac tcgtccctgt atttgtcgag
    10261 gacgggttcg cgcgcgtcga tgacgtacat tgccatatcg ctttgcaaga cttgccgtaa
    10321 gactttggct tcctgattga aatcatggtg cgcgccgtgg ctgccgagaa actgttgcag
    10381 ccgttcgatg ccgtctgaac gattgtccgt atggttttcc agccattcca gcacgccgcc
    10441 tgcgtcttcg agtccgggcg tgtcgtacag gaaaaccagc gtgtctgcgc cgtcgctgat
    10501 ggcggcttct tcgacatgac gcgtggtcga tggggcgttt ctgacttcgc cgaaaccgct
    10561 gtcgcgcaac agggtacgaa ggagcgaggt tttgccggtg ttggtatgcc cgacgacggc
    10621 gagggaaagg ggttgtttgt tcatgatgtt tttgaagaat ggattttcag acggtctttt
    10681 ttcagaatgg cggcttaaca gaacatttca agtgagttta ttggtctttc aaacgccctt
    10741 cctgcgccgc cctgtcaggc tcaagccacg ccgcgccgca ttcggccagc gcgttacgcc
    10801 aatgttccag cttttccgaa aggtcgtctg aaagcccctg ttccgccaaa agctgcacca
    10861 ccgcgccgcc ctgcgccgct tccgaaagtc ggacgatctg ccgcaacacg ccgcggtcgg
    10921 gcacagtttg ggcgcgcacg ccgataagca gttgcgccgg tttctgcttc agctctgtct
    10981 ccagcgcggc aacctgttcc cgattggcgg caacgccctt atccagccat tcctgcgcca
    11041 gcctgccctc gaaccattcg ccgtcctgcc attcggtctc cagcatgacc gcccatttcg
    11101 gcgcatcgtt caagacgatt ttcggcgaaa cggcggacac ggtttcccga cgcgtatccg
    11161 catcggtgat tttgttctgc cagcggcgga tgaccgcctg ataatagggt ttttccaaat
    11221 ccaatccgtt ttcgcttgtt ttcaaaagga ttttgcatac cgcccaagcc aagaggcgcg
    11281 gcaggatgcc gtagcaggcg atactgccga ccagcagccc cgaccaagcc cgcgcatcgg
    11341 caatattgcc gttcagacga ccttcgatga ccgcccgcgc atcaggcacg ggaaaaccca
    11401 gtttcgcagg cagccatgcc aacatttcca ccgcgcgtac cgaagcggca ttgctcaaca
    11461 gcgtgctttc ccagttgaac gtatattgcc gcaccaaaag cagcaacaat accgacacca
    11521 gcattccgag cagcgtgcag agccacaggc tgtgcgacgt tgcgcctatt ttccaacgta
    11581 ccgaaggttg ccgccactcg tccgcataca gccgcaacac cgcctgattt acagggtctt
    11641 tgccccgaaa ccacgtcgcc ggactgctga aaaaacgccc cactttcacg cgcaggaaca
    11701 acattgccaa ccatactgcc agcatcagcg tattcatgcc caacacgccc gccaaaacca
    11761 aaaagaaatt cagaccctga ttgtccatta gaagataagt acctgaaaaa ccggtaaaaa
    11821 acgcaaacgt cgccgccgcc acccacaacc agaacgaccc cgcacgcaca cgttccaacg
    11881 tctcccgcag catacggttc ctgtcaatca tctccgcccg acggatgatt ttttcctccg
    11941 tactgccgtc cacgcggcgc aaagcctccg tcgcctgcac gggatcgccg ctgaaaataa
    12001 aaccgccttc gtccaaaata cggatcagct caaccagttt tcgggatgga ttcaacataa
    12061 aatgccgtct gaaaataaaa aacagatttt aacacacgca ttttcaagaa tattcacagt
    12121 gtaggcaaag agtaaatctc acacagaagc aaaagtatcg gcgtaaactg actgcctcta
    12181 ctttcccgaa agattgtgcg atgtatacag gcgaacgctt caatacttac agccatttga
    12241 gcggtttgat tctggcggcg gcaggtttgg cgctgatgct gctgaaaacc ataggacacg
    12301 gggacggcta ccgtatcttc agcgtatcgg tttacggcat cagccttctt ctgctctatt
    12361 tgagttcctc gctgtaccac ggaattgcag ccggaaaact gaaaagcatt ttgaaaaaaa
    12421 ccgaccactg catgatttat gtcctgattg ccggaagcta cacaccgttt gcactggttt
    12481 ctttgagaaa cgggccgggc tggacggtat tttcactgtc ctggctgctg gcggctgcag
    12541 gaatcgcaca agaactcacc atcggacgga aaagcgaaaa acgactgctg tctattgcga
    12601 tttatgtcgt catgggttgg atggtcttgg cggtaatgaa atccctgaca gcctcactcc
    12661 cgccggcagg actggcttgg ctggcggcag gcggtatgct gtacagcgtc ggcatttact
    12721 ggtttgtaaa cgatgaaaaa atccgacacg ggcacggaat ctggcatctg ttcgtattgg
    12781 gcggcagcat cacccaattt gtcagcgtgt acggttacgt aatctgaatg ccgtctgaaa
    12841 agcaaaacct cccgatcctg aagattggga ggttttctgt ttgccggaca tcagcccttg
    12901 tcgtggaact cgtgaaattc atactgatag gacaaatccc gacccgcttt tttctgtgcc
    12961 aaataatcat cataaatggc acggatttcc ttacgcaaca aaaacagggc tatcaagttc
    13021 gggataacca taaagccgtt gaacatatcg gacaggctcc aaaccaaatc gactttgccg
    13081 agcgtaccca aaacgatggc aagcagaacc aatgcgcgat agatgcccaa gtgtcttccc
    13141 ctgaaaagaa aacggatgtt ggactcaccg aaataatacc agccgataat ggtcgtcaag
    13201 gcgaaaaagg tcagacacac ggcaagcaat tgcgaaccga agcccggaaa tgccttgtta
    13261 aaggcaaatt gagtaaccgc cgcgccctgt tcgcccgaaa ggttggcatc ggtcagcagg
    13321 ataatcagcg cagttgccgt acataccaaa atcgtatcga taaatacacc gacaaatgcc
    13381 gccatacctt gctgcacagg atgcttcaca tccgcagtcg cgtgggcgtg cggggtagaa
    13441 cccatccccg cttcgttgga aaacagaccg cgcgccacgc cgaaacggac ggcttcgcgc
    13501 ataccgatac ccgccgcacc gcccaaaacg gcttcgggat tgaaggcggc ggtaaagatg
    13561 tggttgaaca tcggcacaat atggtcggaa aattcaaaca ggataacgac ggcgcacaaa
    13621 atataaacaa ccgccataaa cggcacgaca aattgggcga tattggcaat acggttcacg
    13681 ccgccaatca caaccatgcc cgcaaggacg gcaagcacaa taccgactgc caaagaaggc
    13741 acatcaaatg caatggtaac ggcagaagca atggagtttg cctgtgtcgc attaccgata
    13801 aagcccaatg cgataatcaa cgcaatggaa aagaaaccgg acaaaaaacg cgccgcgccc
    13861 ctgccgattt tcggagtcag accgtgggtg atgtagaacg ccggcccgcc gatgtatttg
    13921 ccgtggctga cgacgcggta tttctgcgcc agcagtgcct ccgcaaaaat cgtggacatc
    13981 cccaaaacgg cagaaaccca catccaaaaa atcgcgcccg gcccgcctgc ggtgatggcg
    14041 gtcgccacgc cggcgacgtt gcccgtaccg atttgcgcag atatggcaac cgccaacgcc
    14101 tgaaactgcg ataaagactt gtcgtcttta tcgcctttgg caaacaagcc gccgaatacg
    14161 gatttgaatc ccgcgcccag cttggtaatc tgcggcgcac caagatacag cgtaaaaaac
    14221 aggccgatac ccaaaagcgc gtaaatcagc aggtagtccc aaaggaaccg attgactgta
    14281 cccaccagaa cagacaatat attttccata aaataaacct tatcttacaa ttaaaatgac
    14341 tgccttccaa aagacattcc aataaggaaa cacggcgagc agaccgtatt tgccgcaaca
    14401 gatgccttaa attgtcaaca atcggggaga agctgcgcca tcataccgta aaatatcgtt
    14461 aattaaaaca tttctttatt tttaagcgga aagcggagga aatcgctttc agacggcatc
    14521 gacaacggca cggcataaaa caggatattt tgggtacttg caacttatgt taaaatgccg
    14581 accgtaaaaa atctgacaaa aacagattaa ttatttgaaa taagaaagga aatttatggc
    14641 aggccatagc aagtgggcga atatccagca taaaaaagcc cgtcaggatg ccaaacgcgg
    14701 caaaatcttc acccgtttaa tcaaagaaat caccgttgcg gcgcgtatgg gcggcggcga
    14761 ccccggttca aatccgcgcc tgcgcctggc tttggaaaaa gcagccgaaa acaatatgcc
    14821 caaagacaat gtgcaacgcg ccatcgacaa aggtacgggc aacttggaag gcgtggaata
    14881 catcgaattg cgctacgaag gctacggcat cggcggcgcg gctttgatgg tggactgcct
    14941 gaccgacaac aaaacccgca ccgttgccga cgtgcgccac gcgtttacca aaaacggcgg
    15001 taacttgggt actgacggct gcgtggcgtt caacttcgtg catcagggct atttggtatt
    15061 cgaacccggc gttgacgaag acgcgctgat ggaagcggct ttggaagccg gtgcggaaga
    15121 cgtggttacc aacgacgacg gttccatcga agtcattacc gcgccaaacg attgggcggg
    15181 cgtaaaatcc gctttggagg cggcaggtta caaatctgtt gacggcgacg ttacgatgcg
    15241 cgcccaaaac gaaaccgaac tctccggcga cgatgccgtc aaaatgcaaa aactgattga
    15301 cgcgctggaa gacttggacg acgtgcaaga cgtttacact tccgccgtat tgaatctgga
    15361 ctgatacgca gcacagcaga catacaataa aatgccgtct gaacctttca gatggcattg
    15421 atttatttag cccttgccca cgcccaccaa accgtcagga caaccgccag aaaatacgcc
    15481 gcgctccaaa caggcaaagc aacccctaac agataatccg gttcggcaca atttccgaac
    15541 ccgcgcacga caggctcgaa ccaatcaaac aaagaccagc ccttcaagcg gaacgtccac
    15601 ggcgcaccgc acgaaggagc cgtacccggc ggcagcgact gcaaccacaa ctgatatgcc
    15661 gcaacagaaa tacccgtaac ggccggaatg ctgataaaga cagcaccgaa caaaccgccc
    15721 gcccttcttc ttggtctgca catcaggaca attgccgtac acaatgcggt tgccaaaacg
    15781 cataaccgct gactgataca caaaacgcaa ggctccatac ccaaaacata ctgtgccgcc
    15841 aaagaaccgg caaatgcaca gaccgaaacg gcaaacagca gccaaacggc ttttctaaat
    15901 aacggggtca ttttctcaac acaccaatca aaataccgat atgccgattt tgctggatat
    15961 atcccgagat ggcaagggac gaatcgacac cggctcaggt gcggacgatt tcggttttcc
    16021 ccgatatgca caaaacctga caagggacaa aacggcgatt tgcccgcaca atgcccacaa
    16081 aaaaatacat ccggaggatt tgaatttcag caaatttagc gaatcaaaag tttatttcaa
    16141 tgaaatcata tgattttttt gaataagcgg attgatgggt ttttgaagga atttgttacc
    16201 ggatagccat cgggcaagtt ttttgcaaaa tttgaatcgg tcgggtaaat tttcaaaaaa
    16261 taattgacag cggataagaa acggcggata attcccgccg tcgagttgct tgatgcagct
    16321 tgatttttct cctctatttc tcctttgtag acttggcaca cattcaaccg gatgtgtgca
    16381 tttttttatc tccgcttttt ttgaaattta attactttta ttgtttgaaa ttccattctt
    16441 taagaaattt aacaagaaaa acgcttgctt tttgaccgga aaagctgcat aattcgttcc
    16501 gttagctggt tcgagtagtc agttaatagt ttctcctcta tttctccttt gtagacttgg
    16561 cacacattct attgaatgtg tgcatttttt tatctgaagc aacaagcctc tgtgcgtgat
    16621 gttgttatgt ttcatttagg tgtcaaaccg catagccagc ctgaaatatt caatccaaat
    16681 ccaaaaccgg attttctttg acctcctcca tcacaacata actcctactc tccgaagcgg
    16741 caggcagttg caataggata ttgcctagca tatcccgata ggcagacata tcgggcaaac
    16801 gtactttaat cagatagtcg tattcgcccg acaccaagtg gcattccata atttgcggaa
    16861 ttttcagcac ttcttttttg aaatcttcga aaatattgcc cgatttggat tgcagcttca
    16921 gctcgacaaa aaccaatagt ggtttgccca acagatgggg attgagatgg gcgtgatagc
    16981 cggaaatata atgttcccgc tccaaacggc gcaccctctc tgtaacgggc gtggtggaca
    17041 agcctacctt ctcggcaagc tccgtcatcg ggatgcgggc attctgttgg aggatcttaa
    17101 gaatgcggaa atcgatttta tctagttctt tcatttggat taccttgtat ttattattga
    17161 ttttaacaaa tagagt
//
LOCUS       DAXHTR010000029        16755 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.029, whole genome shotgun sequence.
ACCESSION   DAXHTR010000029 DAXHTR010000000
VERSION     DAXHTR010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16755
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.029"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(52..1221)
                     /gene="metK"
                     /locus_tag="ACFNML_000680"
     CDS             complement(52..1221)
                     /gene="metK"
                     /locus_tag="ACFNML_000680"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFC6392096.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGLL
                     PWLRPDAKAQLTVVYDSESGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1404..1514)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM-I-IV-variant riboswitch; SAM riboswitches
                     modulate the expression of downstream genes in response to
                     levels of S-adenosylmethionine; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1567..2463
                     /locus_tag="ACFNML_000681"
     CDS             1567..2463
                     /locus_tag="ACFNML_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6392097.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMRQAGMNPDPKTVKAVFAETAKGGLELAPAFFRKPEDIETMF
                     KAVHGWEHVQQALDKHEGLLFITPHIGSYDLGGRYISQQLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2507..3571)
                     /gene="tsaD"
                     /locus_tag="ACFNML_000682"
     CDS             complement(2507..3571)
                     /gene="tsaD"
                     /locus_tag="ACFNML_000682"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFC6392098.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     GKPVIPIHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVRAENGADDIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGSMTVQIPTPTGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVK"
     gene            3578..3703
                     /locus_tag="ACFNML_000683"
     CDS             3578..3703
                     /locus_tag="ACFNML_000683"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392099.1"
                     /translation="MPLKTEKRILADFGTTAVSQKRNMPSEDRSDGIVAPYQSVP"
     gene            complement(3688..4875)
                     /gene="ccsB"
                     /locus_tag="ACFNML_000684"
     CDS             complement(3688..4875)
                     /gene="ccsB"
                     /locus_tag="ACFNML_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HFC6392100.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRTEGKGGKSWLPQSVLIEEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4868..6820)
                     /locus_tag="ACFNML_000685"
     CDS             complement(4868..6820)
                     /locus_tag="ACFNML_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HFC6392101.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGSFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILGASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7097..7720)
                     /locus_tag="ACFNML_000686"
     CDS             complement(7097..7720)
                     /locus_tag="ACFNML_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6392102.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7926..8555
                     /gene="yihA"
                     /locus_tag="ACFNML_000687"
     CDS             7926..8555
                     /gene="yihA"
                     /locus_tag="ACFNML_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFC6392103.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPEEN"
     gene            complement(8676..11072)
                     /locus_tag="ACFNML_000688"
     CDS             complement(8676..11072)
                     /locus_tag="ACFNML_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HFC6392104.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWAVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11224..12339
                     /gene="pilM"
                     /locus_tag="ACFNML_000689"
     CDS             11224..12339
                     /gene="pilM"
                     /locus_tag="ACFNML_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFC6392105.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKLLYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12342..12941
                     /locus_tag="ACFNML_000690"
     CDS             12342..12941
                     /locus_tag="ACFNML_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HFC6392106.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12942..13589
                     /locus_tag="ACFNML_000691"
     CDS             12942..13589
                     /locus_tag="ACFNML_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HFC6392107.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13607..14152
                     /locus_tag="ACFNML_000692"
     CDS             13607..14152
                     /locus_tag="ACFNML_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HFC6392108.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14171..16456
                     /gene="pilQ"
                     /locus_tag="ACFNML_000693"
     CDS             14171..16456
                     /gene="pilQ"
                     /locus_tag="ACFNML_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HFC6392109.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGGDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
BASE COUNT         4522 a         4757 c         3812 g         3664 t
ORIGIN      
        1 aaaaatgccg tctgaactgt tcagacggca tttttgattt tcaaaccgga attacagccc
       61 cgctgccgcc ctcaatgcag cagctttgtc ggtgcgctcc caagtgaact caggttcttc
      121 gcggccgaaa tgtccgtaag cggcggattt actgtaaatc gggcgcaaga gatcgagcat
      181 ttggacgatg cctttggggc gcaggtcgaa atgttcgcga actaaggtaa tcagtttttc
      241 ctcgctgatt ttgcctgtgc cgaaagtgtc gatggaaatc gaagtcggtt cggcaacgcc
      301 gatggcgtag gaaacttgga tttggcattg ggttgccaaa ccggcggcga cgatgttttt
      361 cgcaacgtag cggcaggcgt aagcggcgga acgatccact ttggacgggt ctttgccgga
      421 gaacgcaccg ccgccgtgcg gagccgcgcc gccgtaggta tcaacgatga ttttacggcc
      481 ggtcaaaccg cagtcgcctt gcgggccgcc gatgacgaag cggccggtcg ggttgatcag
      541 gtatttggtt tcgtcggtca gcagttcaga cggcaggacg ggcttgataa tctgctcaat
      601 gacggcgttt ttcaactctt cataaccgat ttccgggtcg tgttgcgtag agaggacgac
      661 ggtatcaatg cgttttactt tgccggattc gctatcgtaa accacggtca gttgggcttt
      721 tgcgtcggga cgcagccaag gcaggaggcc gtctttgcgt aattcgcttt gacgctgcat
      781 caggcggtgg ctgtaataga tggcaaacgg catcagggtc ggggtttcgt cgcaggcata
      841 gccgaacatc aaaccttggt cgccagcgcc ttggttcaag tcaatgcctt cgccttcgtt
      901 cacgccttgg gcgatgtccg gagattgctg gtcgtagtac acgccgaccg cgcagccgtt
      961 ggcatcaaag cccagctcgg aggagttgta gccgatgcgt ttgatggttt cgcgtgcgac
     1021 tttgatgtag tccacttggg cggtggtggt gatttcgcct gccaatacgc acaagcctgt
     1081 gttgaccaaa gtttctgcgg cgacacgcgc tttggggtct tgcgccaaga tggcatccaa
     1141 aatcgcgtcg gatacttggt cggcaacttt gtccggatgg ccttcggata ccgattcgga
     1201 agtaaacaga tattcgctca ttgagatttt ccttgaaaaa caaaccatct tcttcagacg
     1261 gcatgttgta tgaacataat gtcgacagcg ggaaatatag caaaatttcc ctattcatac
     1321 cattcagttg agaaatattc ccatttgaat ggcactttgg aatctctgcc cgtacgtttc
     1381 ttacaggcaa aaaaattccc gcatcaagcg ggtttggatt gctctggtga gccacatcgg
     1441 cttttcaacc gtccacctta cttttccttt tgaaaagcag gttggcatgg aattcccaac
     1501 tcttaatgca gcgcggatgg tagcagaaaa gcctttccgc cgcaatactt ccctttttca
     1561 gacggcatgt ttcgtttaca attcgggctg tttccccctt tgcgaaccgc catgcacatc
     1621 ctgttgaccg ccctgctcaa atgcctctcc ctgctgccac tttcctgtct gcacacgctg
     1681 ggaaaccggc tcggacatct ggcgttttac cttttaaagg aagaccgcgc gcgcatcgtc
     1741 gccaatatgc gtcaggcagg catgaatccc gaccccaaaa cagtcaaagc cgtttttgcg
     1801 gaaacggcaa aaggcggttt ggaacttgcc cccgcgtttt tcagaaaacc ggaagacata
     1861 gaaacaatgt tcaaagcggt acacggctgg gaacatgtgc agcaggcttt ggacaaacac
     1921 gaagggctgc tattcatcac gccgcacatc ggcagctacg atttgggcgg acgctacatc
     1981 agccagcagc ttccgttccc gctgaccgcc atgtacaaac cgccgaaaat caaagcgata
     2041 gacaaaatca tgcaggcggg cagggttcgc ggcaaaggaa aaaccgcgcc taccagcata
     2101 caaggggtca aacaaatcat caaagccctg cgttcgggcg aagcaaccat cgtcctgccc
     2161 gaccacgtcc cctcccctca agaaggcggg gaaggcgtat gggtggattt cttcggcaaa
     2221 cctgcctata ccatgacgct ggcggcaaaa ttggcacacg tcaaaggcgt gaaaaccctg
     2281 tttttctgct gcgaacgcct gcctggcgga caaggtttcg atttgcacat ccgccccgtc
     2341 caaggggaat tgaacggcga caaagcccat gatgccgccg tgttcaaccg caatgccgaa
     2401 tattggatac gccgttttcc gacgcagtat ctgtttatgt acaaccgcta caaaatgccg
     2461 taacgaaaat aaaaatgccg tctgaacaat ttcagacggc attttgtcat ttgacgattt
     2521 ccgacagcgg ccagcgcgga cggacattga acgcaccgac ctccctgccc ttgcccaggt
     2581 gcatcgcacc ggcaaaggca atcatggcac cgttgtccgt gcagtatgcc gtcggcggga
     2641 aaaacacgct gactttttcg gacggatgtt tcggcttgcc tgttggggtc gggatttgca
     2701 ccgtcatgct gccgaaagtt tcacggagtt tgcggtttgc accgaccccg ccggcgacca
     2761 ctacggttct gaaccctgtc tgcaacaggg cttttttcac tttcgccgcc aacacatcga
     2821 ctaccgcatc ttgaaacgca cggcagatgt cgttgcgtgt ctgctcagga atgtcatccg
     2881 ccccgttttc cgcgcgcact ttctcgacgg cagtcaaaac agcggttttc aaacctgaaa
     2941 aactcatctg caaatcgtcg gaatgaatca tcgggcgcgg aaaaacaaac gcttcgggcc
     3001 tgcccgattc cgcaagttcc gacagtttcg caccgcccgg atacggcaag cccagcagtt
     3061 tcgccgtttt gtcgaatgcc tcgcccgccg catcatcgac gctctcgccc aaaagcgcgt
     3121 agtcgcctat gcccctgacc gccataatct gcgtatgccc gcccgaaacc agcagcgcga
     3181 caaaaggaaa gtcgggtttt tcctccgcca acagcggcga cagcagatgt ccttccaaat
     3241 gatggatggg gatgacgggc ttgcccaatg ccaaagccag tgcattggcg tagctcgaac
     3301 ccgccagcag cgcaccgccc agaccgggtc cctgcgtaaa ggcaaccgcg tcaatgtcgc
     3361 catacgatgc acccgcctgc gccaaacagc cttccgtcaa cggaacaagg cggcggatat
     3421 ggtcgcggct tgccaattcc ggcacaaccc caccgtattc ggcgtgcatt gccatttgag
     3481 tgtgcaggca gtgcgcccgc aatcctcgtt ccgtatcgta aagcgcaacg ccggtttcgt
     3541 cgcaagaaga ctcgattcct aataccaaca tggtctgatg ccgttaaaaa ctgaaaaacg
     3601 tattttagcg gatttcggca cgactgccgt atcccaaaaa cggaacatgc cgtctgaaga
     3661 ccgttcagac ggcatcgtcg caccgtatca aagcgttccg taagaatgca gcccgctcaa
     3721 aaacatattc acgccgataa aggcaaatgc ggttacgaac aaaccgataa tcgcccacca
     3781 cgccagcact ttgccgcgcc aaccggcaac cagccgcaag tgcagccaaa cggcgtaatt
     3841 gagccagacg atgaacgccc acgtctcttt cgggtcccaa ctccaatagc gtccccaagc
     3901 atccgccgcc cacagcgcac ccaaaatggt ggcaatggta aagaacagaa agccgacggc
     3961 aatcgcctta tacataactt cctcaatcaa cacggattgc ggcagccacg atttcccgcc
     4021 ttttccttcc gtacgcaggg aaaccagttc ggcaataccg agcatcgcgg aaatgcaaaa
     4081 cgcgccgtaa ccgataaagt ttgccggaac gtggattttc atccaccaag attgcaatgc
     4141 cggaatcagc ggctggatgg tatgcgcctc gcgggacacg ctgtaccaca agacaaatcc
     4201 aaccacgacc gccataaagc cgaacacgaa gccgcccaat ttctgtatgg cgaacttacc
     4261 ttcataataa agatacatca gcgcggtaat caccaaaaac aggatgaaca cttcatacag
     4321 gttggaaacc ggaatatgcc ccgcatcggg acggagcaga tagctttcgt gccaacgtac
     4381 cagcagaccg gtaaagcctg ctacggcaga cacccatgca aacacggttc ccatacccaa
     4441 cagcgtgttg gtcggcacat tttttacgct tgccaaaacc gcgcccgaaa tataggcgaa
     4501 cagggcgaag aagacaaagg cgcactgcca cataatcgcc gactggctgc tgaggaaata
     4561 ccgcaacagg aaaatctctg ccgatttgat gttgccgcca tacaaaccga cggcggcata
     4621 ggcaagcaat acgcttaaag gaacaaacca gcgcatcggc ttgaaaaacc agcccaaaaa
     4681 tacggcaata ccggcactcg cccacaacat gaccgtttcg taaatgtcca tatgcatacc
     4741 ggaacgggtc tgtacgaaaa ccgtagccgc aaaaaccagc acggcaaata cccaatccca
     4801 aagattcaga ttgctgatca aagacttctg aatcagcagc tcgtgttccg gaagggtttt
     4861 atagtgttca gtcatgattc aagtccttgc cgagccgttg cagactctcg acgtgttttg
     4921 gaaattcctt ctgcaaatcc cgttcgctgc gggccgaaga catggcaaaa cggattttgc
     4981 cgtctgaaaa caatacccac gcccgttttt cgcgcacata aaacatcaat accgtaccca
     5041 ataccaacag caccgagccg agatagacca aaagcgcacc cggggaacgg gtcatctgca
     5101 aacccgacga acgcacctcg gaaaacccat caagttgcag cagcataggc gcgggatatt
     5161 cggtcaaacc cgtgtacgca tccatactgt gcagcaggaa acggttccgc gcttcatcct
     5221 gctgccattc gggcaagccg taccggcgta tggtttcatc caaagcagcg ttcatcacgc
     5281 cgtaaagcat ttcgtagaaa taaccctgca tcttatcctg ctgctctttc ggaatattgg
     5341 acgtaataaa ttcgtccaat cccaaatagc ctttttgtgc aaagatgttc agcgtgtttt
     5401 ccgcagccag catgaattgt tcgcggattt cggcaggtgc gtctttggtt gcgtcggcaa
     5461 ccagacgttt gcgcccttcc ccatctttca aaaactcgcg caatgccata aaggtgtccg
     5521 ctttcaactg cttgtccaag gggatacgca gccagcggta ttgctgctgc aagccgctgc
     5581 gcgtgccggt aatccaaaaa taatcctgtt cctgcaaaac cggcagcata tagtttttat
     5641 attcgaccgc ctgccctgcc gcatcacgga tacggtaaac aatggaaggg ccgatattgg
     5701 tgtatttttt accttcctga gtaacggcgc ggacatcgtt cagcgtggat ttcaggcttt
     5761 tttcccgttc cgcgccctcg ctcatgtcct ccacattcat agaagtgaac tgatcgaact
     5821 caagacgata tttgtgtttg ccaatttcca acggaaactg gtgtatggat gttgccttca
     5881 acacgacagg ctcgcgcgaa gcatcaccca aattccacgc cttgaatgtc aaatccgaac
     5941 cgccgtcggc aaaactcgcc tgataaatcg tgatgccgtg caaggtcaaa ggatggttca
     6001 cgcggatggt gcgctcgagt ttctcaccgg ttgccttatc cgttacttca atatcactgg
     6061 caaaatcgcg cggcataccc gtattgtaaa aatcgatatg gaattttttc agtttgactt
     6121 caaaaggcaa gtcctgaacc aatatcccgt tgtcggcatt caggaaaacc acatccgcac
     6181 tctgcccctc ggaaatattg acgttgcccc taaatgagag attggacgca cccaaaatac
     6241 tttcgggctt gaaatccttg gcataaaccg cctgattgtc cggaacaatc cgaccggtca
     6301 gcatacccag tttcaacagc aggttactgt ctatcaaccc gcccaggcaa atgacaatca
     6361 aagcaacatg ggcaaagata tagccccatt tgttcattgt gccttttttg gcggcaatca
     6421 gaaccgaccc gtcttcacgg ttaatggttt ttccctgaaa accttgtact tccagataac
     6481 gtttggcaac ctcgggcgca atttttacat ccaacagcga agaatggcgc atcgccgcca
     6541 gagatttttc tttaaccttt tcccgaaaag acttcatttc gcgccagaac ggcggcacat
     6601 tgcgaatcag gcacaaactg gtagaaacca ccaaaaacat catgataacg acaaaccatg
     6661 ccgaagcata gacgtcatac agtcccagaa aaccaaaaat ctgcgcccaa aacgatccga
     6721 atttgaccaa ataatccgtc tgcggctggt tttgctgcaa caccgtaccg ataaccgatg
     6781 caatacccag cagactgagc aaagcgactg caaagcgcat ggagctgaaa aaagcgaacc
     6841 acggacggga aagaagtggg ggagatctac gggatttact cattgtgtgt ttattccgcc
     6901 atcagaaata tgggaaagca gaattgggca aacagaaaac aacgtcccga ttctactgtc
     6961 ttgatgcttt ttatttcaag acaatgaaga cagcctgcat cgattccaac ggttgcgatt
     7021 gaaaaaactt atcgcagaat tgcctgaagc cgtctgaaaa cttttcagac ggcctctaaa
     7081 acagactatt gcggaattaa cgcaaacctt ggataaagtt ggcgaccgct ttcaaatctt
     7141 cttcagacat acggtttgca atatcttcca tgatggtatt tttacgctga ccggacttgt
     7201 aggcattcat ctgttcaaca atatatgcct gatgctgacc gcccaaacgc ggataagcct
     7261 gaatttcgct tccgcctccc ggcatacccg caccgctcgg accgtggcag gacatacacg
     7321 ccggcacttt tttatcgctc aaaccgccgc gatagatttt cgcacccaat tcgggatttt
     7381 ccttaggatt ggcttcaccg gatttgggct gctgtttggc atagaatgcg gatacgttca
     7441 aaatatcctg atcgctcaaa ttcattacca ccggtttcat cacagctgcc gaaccgtggg
     7501 tgcgtttacc gtcgcggatg ccgatagttt gatgatagat gtaagcagta tgctgtgccg
     7561 ccaaacgcgg atacatcgca atgccgctgt taccgtctgc tgcatggcaa gccgcacaaa
     7621 ccgttgcggc aacctgtttg cctttttcca cgtctgcttt gggagaggcg gaaaccgcac
     7681 cggcagccaa aacaaaggcc aataaagtca atcgtttcat ggagtgctcc tgattacagc
     7741 attggataac gcaacaatgc tctttttata ttcaaatacg ggatttttga cccgattaaa
     7801 accgatgatt ctgtaaacgt gttattctat actaaattta cattaaatta ccactgtgtt
     7861 tcacataaaa ccaaccgcat atttttgctg tcggacaaac ggcggcggaa aacaaggata
     7921 tgcccatgaa cctttttcaa aacgccaaat tcttcacgac ggtcaaccac cttaaagacc
     7981 tgcccgacac ccctctcgaa attgcctttg tcggcaggag caatgccgga aaatccagtg
     8041 ccatcaatac cctgaccaac catgtccgtc ttgcctacgt ttcaaaaaca cccggacgga
     8101 cgcagcatat caacttcttc gagctgcaga acggcaattt tatggtcgat ttgcccggct
     8161 acggttatgc ccaagtccct gaagcagtac gcgcacattg ggtcaatctg ctcggcgact
     8221 atctgcaaca gcgcaaacag cttatcgggc tggttttgat tatggatgcc cgccatcctt
     8281 taaaagaact cgacatccgt atgctggatt ttttccacac gaccggcaga ccggttcaca
     8341 tcctgctgtc aaaagccgac aaattatcca aaaacgaaca gataaaaacc ctgtcccaag
     8401 tcaaaaaact gctcaaacct tattccgaca ggcaaaacat cagcgtacag ctgttttcca
     8461 gcctgaaaaa acaaggtatt gacgaggcca accgaactgt cggaagctgg tttgacgcag
     8521 cagatgccgc cgcttcctct ccagaggaaa actgacccca attatacgga aaccgtattt
     8581 cccccacttg accgaccgca aacatttaaa aaattgccac tgccaaatct aaaatgccgt
     8641 ctgaaagact tttcagacgg cattttgcgg agtctttaaa acagagaatc caactgctgc
     8701 tgtttggaac cagtattact cggaagcacc ggcgtttcct gcatatcttg gcggacttcg
     8761 tcatccgccg cctgccgtcc gccttctgcc gcgcccccgt catcttcttt tgcccgtcgg
     8821 gaaggttgcg gcgcaatacc gctgttgtcc agcgtcaagc ccggatcggt taccatacgt
     8881 tccttcatat agtattcgcc attgctgctg accacacctt caggcatttt catccccttg
     8941 ccctgctttc ctttcaacgc aaaacgcata tagtccaccc aaaccggcac cgcaatcgta
     9001 ccgccgtagc cgacacgccc catactctta ggtttgtcga agccgatata tacggcagta
     9061 accacatcag ggttaaaacc gacaaaccac gcatccttat tgtcattggt cgtacccgtt
     9121 ttaccggcaa tatccgttct tcccaacgca gctgcccccc ttgccgtacc gacacggacc
     9181 acatcctgca taatcttata cataatatag gcattgcgcg gatcgattgc ctgaggcgca
     9241 ttttgcccgg ccaccaaagg ttgcatttgg gcgcgcaacc tgccgtctct gtcataaatc
     9301 ttatcgatta cgtgcgaaga aaccctatat ccgccgttcg caaatacgct atatgcctcc
     9361 gccactttca acggcgttgt ctcgcccgta cctaaagcca tagacaggct tgccggcagc
     9421 tcggacgacc tgaagccgaa acgccggata tactgttgcg cgtaaccgac accgatagac
     9481 atcaaaatac ggatggaaac catattcttg gaagccgtca gagcctgtct caaagtaatg
     9541 tagccggaat atctgccgtc tgaattttta ggtgtccaaa ccgaaccgtt cggccctttc
     9601 cccggcaggg aaatcggcgc atcgttaacc accgtggacg cggtcatccc cttagataat
     9661 gccgccgaat agacaaacgg cttaaaggtc gaacccggct gccgcattgc ctgaacggca
     9721 cgattgaatg ttttgctgtg aaaatcataa ccgccgacca gcgcgcgcac agctccggtt
     9781 tttgcatcca gcgaaaccaa agccccctgc agcaacggct cttgaaccac cgcccaacgc
     9841 ccgccgttgt ttttgacacg gatgaccgcg cccctgcgga tacggtcctc ccccattttt
     9901 tcattattga ccgcgcgggc cgcaaagccc aaggcgcgcc tgtcaagcgt aacccgcctg
     9961 ccgccgggca gctgtatgac gacatttttc tttttagtca catccaacac aacggcggga
    10021 accattttat cgacggtata gagtcccgac agatactggc tgacagtctc ctcgacatct
    10081 tcactcttac tcaaatcgat atagttttcc gcaccgcggt agctgctgcc gcgatcgaaa
    10141 ttccgtagag ccttgcgcaa tgcctcggtt gccaccttct gatgatcggc gcggaccgtg
    10201 gtataaacct taaaaccctg cgtataggca tcttcaccgt atttctcata cagttcctga
    10261 cgcaccattt ccgccacata taaagcactc tgatcgattt tccgaacaaa ccgctcgtaa
    10321 tgcagttcct cattcaacgc ctgatcgcgc tgttgcacgg taatcatctt ctcctcgagc
    10381 atattgttca aaatatactt ctggcgcaac ttggcacgtt ctggattaac aatcggatta
    10441 taggcagacg gagccttggg cagtcccgca agcatggcgg cttccgccaa agtcaaatct
    10501 cggacattct tattgaaata gatttgcgcg gcagatgcaa aaccataggc gcgctgaccg
    10561 aggtaaatct gattgaaata caactcgagg attttgtctt tgcttaaaga ctgctcgatt
    10621 ttataggcaa gcaacacctc attgaatttg cgtgtgaacg ttttttcact gctcaaataa
    10681 aaatttttcg ccacctgctg cgtaatcgta ctcgcacccg actgcacgct gccggacacg
    10741 acattgccga cggcagcgcg ggcaacaccc caaacatcca ccccccaatg ccggtaaaag
    10801 cgtttatcct cggcggcgat aaccgcattc cgcaacacct ctgggaaatc gccgattttt
    10861 gtaaattcgc gccgctgctc cccatacata ccgatgactt ccccatccgc cgaataaata
    10921 gtcaacggca ttttaggctg gtaatgctgc aaagaatcca aagacggcag tttcggatac
    10981 gttaccaaaa tagcaatggc aaccaaaccc actccaaata cacaaaaccc aaaaaccaaa
    11041 ccaaaacaag tcgttaaaat ctttttaatc atagctgaat aataatttac cattattggt
    11101 attaaataaa gtaaaatagc aaccgatttc tacaaagcac ggtttcaatg tgcaaagaac
    11161 aaggaatcca ttacggatac cgaaacggtt actcactgta caaataaagc aggaactttc
    11221 atcatgcgct tgtttaaaag cttgaaaaac cctaaaaaaa cagatgccaa gctccctaaa
    11281 aaatcttcgg gactcaataa ccgcgcggca atcggcatcg atatcgacca gcattccatc
    11341 aaaatggtcc aattgtcagg acgtagttta aaccaaattc aattggaaaa atacgtcatt
    11401 gccaaattac caaagaatat cattcaaggc aataaagtcc aaaattacga tcaacttgtt
    11461 acatatttgc aacaagccta tgccaaactg ggtacttcgt gcaaaaacat catcgcgtcc
    11521 gtcccgcaaa atctggcaac catcgaacaa ttgacctaca cagacaaaga tgcagaatta
    11581 gacctgcagg ggttcgtgga gtcctccatc tccgaagtca gctcgatatc gctcgaagaa
    11641 gccaattacg actatcaggt cttgtcccaa tcggccgccg gcgaagctgt gttggccgtc
    11701 gcatcgagaa aggatgaaat cgaacccctg attgacgcat tcaacgccgc cggtatgaaa
    11761 ttatccgcgc ttgatgtgga cattttcgga caatacaacg cctacgcgct atggataaac
    11821 catttcgccc ccgagcttgc ggccgaaaaa gtcgccattt tcggcgtata tgccgcacag
    11881 acctacgcct tggtcatcca agacggaaaa ctcctataca aacaggaaac ctccgtcagc
    11941 gaagaacagc tcaaccaact catccagcgc acctatcagg taacagaaga aaaagcggaa
    12001 gaaatcatca actccccgca aaaaccttcc gattaccaag aaagcgtggc aaactatttc
    12061 aaccagcaga ttacccaaga aatacaaagg gtcttgcagt tttattacac cacgcagacc
    12121 gcagacgata tgaccgacat caagcatatc ctgctgaccg gggaagcggc gcgccaggaa
    12181 ggcatcgccc aaaccgtcgc ctcacaaacc aatgcagatg tacaatgcgt ccatcccgcg
    12241 cgttattttg cggacaacct caaaacagac aaacaacaat tcgaacttga tgcgccgaca
    12301 ctgaccaggg cgttcggttt ggcggtacgg ggattataat tatgaacaat ttaatcaaaa
    12361 tcaacctcct cccctacagg gaagagatga acaagcgcaa acagcagcag tttaaaacgc
    12421 tgatgtacgg tgccgtgctg acgggcgttg ccgccgttgc ggcaacctac ctgtttatcg
    12481 acaatatgat caataaccag tcggaaagaa acacgctgct ggaaacctcc atcgcacact
    12541 tggataccga gctgtcggaa atacaaaagc tcaaacagga aaaagatgcc ttcctgatta
    12601 agaaaaacaa aatcgaggag ctccagctca aacgcctcca agccgcaaaa atcctcgaca
    12661 gcctgaatga ggccgtcccc ggaagcacct acctgacctc gctggatgcc gttaccgccg
    12721 actcttatcg gctcagcggc aggacatcca gcgacaaccg cgttgccgcc atgatgaggg
    12781 cgatgcccaa taccggcata ttcaagcaac ccgaattgtt aagcatcaag aaaaacaatt
    12841 cgcatcaaga atttaccctt caggcaacat tacaacccat cgtaaaggcg gccgaatcca
    12901 aagagaatcc ggcttcggga aacgcacagg aggcaaactg aatggcttct aaatcatcta
    12961 aaaccaactt ggatctcaac aaccttcacc tgctcaacct tcctgccagg ctttttatcg
    13021 ccctgctggc cgttgccgcc gtgctggggc tcggttatgc cggattgttc aaaagccaga
    13081 tggaatccct tgaggaatac gaagcaaaag aaaccgaact gaaaaacacc tacaaacaga
    13141 aaagtatcga cgcggccagc ctgaacaacc tgagggacga acttgcctca atccgctctg
    13201 ccttcgatat catgttgaaa cagctgccga cagatgcaga aattcccaac ctggttcaag
    13261 agcttcatca ggcaggttcg agcaacggcc tgcgcttgga cagcgttatg ccccaacctc
    13321 ccgtagatga cggccccatc aaaagattac cctattccat ttccattacc ggaaattacg
    13381 aacagatcag ccaatttacc cgcgatgtcg gcagcctctc ccgaatcatt acccttgagt
    13441 cgctgaaaat cgcccaatct ccggaaaacg gcggcaatcc tgacggcaag agcagcatcc
    13501 tgaacctcag tgccattgcc accacctacc aagcaaaatc cgtagaagag cttgccgcag
    13561 aagcggcaca aaatgccgag caaaaataac ttacgttagg gaaaccatga aacactatgc
    13621 cttactcatc agctttctgg ctctctccgc gtgttcccaa ggttctgagg acctaaacga
    13681 atggatggca caaacgcgac gcgaagccaa agcagaaatc atacctttcc aagcacctac
    13741 cctgccggtt gcgccggtat acagcccgcc gcagcttaca gggccgaacg cattcgactt
    13801 ccgccgcatg gaaaccgaca aaaaagggga aaatgccccc gacaccaagc gtattaaaga
    13861 aacgctggaa aaattcagtt tggaaaatat gcgttatgtc ggcattttga agtccggaca
    13921 gaaagtctcc ggcttcatcg aggctgaagg ttatgtctac actgtcggtg tcggcaacta
    13981 tttgggacaa aactacggta gaatcgaaag cattaccgac gacagcatcg tcctgaacga
    14041 gctaatagaa gacagcacgg gcaactgggt ttcccgtaaa gcagaactgc tgttgaattc
    14101 ttccgacaaa aacaccgaac aagcggcagc acctgccgca gaacaaaatt aagaagagga
    14161 ttactccatt atgaatacca aactgacaaa aatcatttcc ggtctctttg tcgcaaccgc
    14221 cgcctttcag acagcatcgg caggaaacat tacagacatc aaagtttcct ccctgcccaa
    14281 caaacagaaa atcgtcaaag tcagctttga caaagagatt gtcaacccga ccggcttcgt
    14341 aacctcctca ccggcccgca tcgccttgga ctttgaacaa accggcattt ccatggatca
    14401 acaggtactc gaatatgccg atcccctgtt gagcaaaatc agtgccgcac aaaacagcag
    14461 ccgtgcgcgt ctggttctga atctgaacaa accgggccaa tacaataccg aagtacgcgg
    14521 gaacaaagtt tggatattca ttaacgaatc ggacgatacc gtgtccgccc ccgcacgccc
    14581 cgccgtaaaa gccgcgcctg ccgcaccggc aaaacaacag gctgccgcac cgtctaccaa
    14641 gtccgcagta tccgtatcca aaccctttac cccggcaaaa caacaggctg ccgcaccgtt
    14701 taccgagtcc gtagtatccg tatccgcacc gttcagcccg gcaaaacaac aggcggcggc
    14761 atcagcaaaa caacagacgg cagcaccagc aaaacaacag gcggcaacac cagcaaaaca
    14821 aaccaatatc gatttccgca aagacggcaa aaatgccggc attatcgaat tggctgcatt
    14881 gggctttgcc gggcagcccg acatcagcca acagcacgac cacatcatcg ttacgctgaa
    14941 aaaccatacc ctgccgacca cgctccaacg cagtttggat gtggcagact ttaaaacacc
    15001 ggttcaaaag gttacgctga aacgcctcaa taacgacacc cagctgatta tcacaacagc
    15061 tggcaactgg gaactcgtca acaaatccgc cgcgcccgga tactttacct tccaagtcct
    15121 gccgaaaaaa caaaacctcg agtcaggcgg cgtgaacaat gcgcccaaaa ccttcacagg
    15181 ccggaaaatc tcccttgact tccaagatgt cgaaatccgc accatcctgc agattttggc
    15241 aaaagaatcc gggatgaaca ttgttgccag cgactccgtc aacggcaaaa tgaccctctc
    15301 cctcaaagac gtaccttggg atcaggcttt ggatttggtt atgcaggcac gcaacctcga
    15361 tatgcgccag caagggaaca tcgtcaacat cgcgccccgc gacgagctgc ttgccaaaga
    15421 caaagccttc ttacaggcgg aaaaagacat tgccgatctg ggcgcgctgt attcacaaaa
    15481 cttccaattg aaatacaaaa atgtggaaga attccgcagc atcctgcgtt tggacaatgc
    15541 cgacacaacc ggaaaccgca atacgcttgt cagcggcagg ggcagcgtgc tgatcgatcc
    15601 cgccaccaat accctgattg ttaccgatac ccgcagcgtc atcgaaaaat tccgcaaact
    15661 gattgacgaa ttggacgtac ccgcgcaaca agtgatgatt gaggcgcgta tcgtcgaagc
    15721 ggcagacggc ttctcgcgcg atttgggcgt taaattcggc gcgacaggca agaaaaagct
    15781 gaaaaatgat acaagcgcat tcggctgggg ggtaaactcc ggcttcggcg gcggcgataa
    15841 atggggggcc gaaaccaaaa tcaacctgcc gattaccgct gccgcaaaca gcatttcgct
    15901 ggtgcgcgcg atttcctccg gtgccttgaa tttggaattg tccgcatccg aatcgctttc
    15961 aaaaaccaaa acgcttgcca atccgcgcgt gctgacccaa aaccgcaaag aggccaaaat
    16021 cgaatccggt tacgaaattc ctttcaccgt aacctcaatc gcgaacggcg gcagcagcac
    16081 gaacacggaa ctcaaaaaag ccgtcttggg gctgaccgtt acgccgaaca tcacgcccga
    16141 cggccaaatc attatgaccg tcaaaatcaa caaggactcg cctgcgcaat gtgcctccgg
    16201 taatcagacg atcctgtgta tttcgaccaa aaacctgaat acgcaggcta tggttgaaaa
    16261 cggcggcaca ttgattgtcg gcggtattta tgaagaagac aacggcaata cgctgaccaa
    16321 agtccccctg ttgggcgaca tccccgttat cggcaacctc tttaaaacac gcgggaaaaa
    16381 aaccgaccgc cgcgaactgc tgattttcat taccccgagg attatgggta cggccggcaa
    16441 cagcctgcgc tattgatgcg tcaaaataag ggcatatgtt ttacggcata tgccctttct
    16501 ttatgctttt tgccgcgacc gaaatgccgt cattcccgcg caggcagaaa tccaacttgt
    16561 ccggttccgg ttgtttttcg tctcgtaact tttgagccgt cattcccgcg caggcagaaa
    16621 tccagtccgt tcagtttcgg tcatttccga taaattcctg ttgcttttca tttctggatt
    16681 cccacttttg tgggaatgac ggcggagagg tttttgttgt ttcggagaag tttctgcaac
    16741 cctagaatct cgtta
//
LOCUS       DAXHTR010000030        16675 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.030, whole genome shotgun sequence.
ACCESSION   DAXHTR010000030 DAXHTR010000000
VERSION     DAXHTR010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16675
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.030"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..744
                     /locus_tag="ACFNML_000694"
     CDS             <1..744
                     /locus_tag="ACFNML_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6392110.1"
                     /translation="KLGVKTGLSIDKNRLPYLDEYYKTTKPAGTLFEPDYEALNAYKP
                     QLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDALAQIFGKQAEADKLKA
                     EIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWLHKDIGVPAVDEAIKEG
                     SHGQPVSFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLDNPLVAETTAWKKGQVVYL
                     VPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            908..1877
                     /locus_tag="ACFNML_000695"
                     /pseudo
     CDS             908..1877
                     /locus_tag="ACFNML_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1867..2841
                     /locus_tag="ACFNML_000696"
     CDS             1867..2841
                     /locus_tag="ACFNML_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HFC6392111.1"
                     /translation="MPSEKNIGFMAGSSRPLWVAFALLLVSCVLFMTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYASLPLTGKFGFELAVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLP
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            2886..3512
                     /locus_tag="ACFNML_000697"
     CDS             2886..3512
                     /locus_tag="ACFNML_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HFC6392112.1"
                     /translation="MTQERLPEFFDRAPTLTVQDPLAAFLGAAENGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKALYGEELPERGGIEAFMQGARDEGTVGVTASVVQLLTG
                     AAPETGFGGVGPAGRFARRNLLSFGAGEINGTLALRRRDTGKTVAVSLNAALQPFAPE
                     MRELMPKTVGGSASAEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3596..4354
                     /locus_tag="ACFNML_000698"
     CDS             3596..4354
                     /locus_tag="ACFNML_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6392113.1"
                     /translation="MITIRNVSYRIGTRPILDNVSLDIPEGGITALVGPNGAGKSTLF
                     SFMARLRPLESGSIAYRGKNLACTPTAELAKTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPTAECRRIVNGAIEEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVVVLHDINQAAAYADHVVAMKNGQVAMQG
                     KPDDIFTAANIKTLFDMDVSVLDHQSKKLIVHHI"
     gene            complement(4413..4751)
                     /locus_tag="ACFNML_000699"
     CDS             complement(4413..4751)
                     /locus_tag="ACFNML_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFC6392114.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            4846..5058
                     /locus_tag="ACFNML_000700"
     CDS             4846..5058
                     /locus_tag="ACFNML_000700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392115.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRTETTAFDLKT
                     YGSSCPPLYFLQLSAYPALGKNQKNR"
     gene            5215..9177
                     /gene="purL"
                     /locus_tag="ACFNML_000701"
     CDS             5215..9177
                     /gene="purL"
                     /locus_tag="ACFNML_000701"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFC6392116.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFAG
                     SEKALDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGRLNDDEKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPCLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPVDLPLNVLLGKPPKTTRTDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFANLKFDVNEDIAAPFINSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRFRLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPDTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGNISADLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEGWTELSGWYRLFAGARKALG
                     "
     gene            9272..10030
                     /gene="gloB"
                     /locus_tag="ACFNML_000702"
     CDS             9272..10030
                     /gene="gloB"
                     /locus_tag="ACFNML_000702"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFC6392117.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10337..14810
                     /locus_tag="ACFNML_000703"
                     /pseudo
     CDS             10337..14810
                     /locus_tag="ACFNML_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
     gene            15042..16496
                     /gene="mgtE"
                     /locus_tag="ACFNML_000704"
     CDS             15042..16496
                     /gene="mgtE"
                     /locus_tag="ACFNML_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HFC6392118.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLGELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRVTVDEMVDVIREESEADMLNMAGLQEEEDLFAPVLDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
BASE COUNT         4030 a         5149 c         4198 g         3298 t
ORIGIN      
        1 aaattgggcg tgaaaaccgg tttatccatc gataaaaacc ggctgcctta tttggacgaa
       61 tattacaaaa ccacgaaacc tgccggaact ttgttcgagc cggattacga agccttaaac
      121 gcctacaagc cgcagctcat catcatcggc agccgcgccg ccaaggcgtt tgacaaattg
      181 aacgaaatcg cgccgaccat cgaaatgacc gccgataccg ccaacctcaa agaaagtgcc
      241 aaagagcgta tcgacgcgct ggcgcaaatc ttcggcaaac aggcggaagc cgacaagctg
      301 aaggcggaaa tcgacgcgtc ttttgaagcc gcgaaaactg ccgcgcaagg caaaggcaaa
      361 ggtttggtga ttttggtcaa cggcggcaag atgtcggctt tcggcccgtc ttcacgactg
      421 ggcggctggc tgcacaaaga catcggcgtt cccgctgttg acgaagccat caaagaaggc
      481 agccacggcc agcccgtcag ctttgaatac ctgaaagaga aaaatcccga ctggctgttc
      541 gtcctcgacc gaagcgcggc catcggtgaa gagggtcagg cggcgaaaga cgtgttggat
      601 aatccgctgg ttgccgaaac aaccgcttgg aaaaaaggac aagtcgttta ccttgttcct
      661 gaaacttatt tggcggccgg tggcgcgcaa gagctgctga atgcaagcaa acaggttgcc
      721 gacgctttta acgccgcgaa ataatgaaac ggcggcattc gatgccgtct gaaacacgga
      781 tgcaaaccgc ttcctgtgtt tcagacggca ttgcccgata cggaggcttc aaacaaggct
      841 ttccgctccg acggttcgga ctgccttgtt tgaatcttct acgccgtaac gcttttccct
      901 tctgtttatg actgccaaac ctttttccct caacctgact aacctcctgc tgctggcggt
      961 gttgtttgcc gtcagcctgt cggtcggcgt tgccgatttc cgctggtcgg atgtgttttc
     1021 gctgtccgac agccagcagg ttatgttcat cagccgcctg ccgcgcacgt ttgcgattgt
     1081 gctgacgggc gcgtcgatgg cggtggcggg gatgattatg cagattctga tgcgcaaccg
     1141 ttttgtcgaa ccttctatgg cgggcgcgag ccaaagtgcg gctttgggtt tgcttctgat
     1201 gtccctgctg ctgcctgccg cgccgctgcc ggtcaaaatg tcggttgccg ccgttgccgc
     1261 gctaatcgga atgctggtgt ttatgatgct tatccgccgc ctgccgccga cggcgcaact
     1321 gatggtgccc gctggtgggg attattttcg gcggcgtggt tgaggcggtg gcgaccttta
     1381 tcgcgtatga aaacgaaatg ctgcaaatgc tcggcgtgtg gcagcagggc gatttttcga
     1441 gcgtgctgct ggggcggtac gagctgcttt ggattacggg cggtttggcg gtgtttgcct
     1501 atctgattgc cgaccggctg acgattttgg ggctgggcga gacggtcagc gtgaatttgg
     1561 gtttgaaccg gacggcggtg ttgtggtcgg ggctgattat tgtggctttg attacgtcgc
     1621 tggttatcgt tacggtcggc aatattccgt ttatcgggct ggtcgtgccg aacatcatca
     1681 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtggctttg ttgggcgcgt
     1741 ctttggtttt attgtgcgat attttgggac gcgtgattgt gtttccgttt gaaattccgg
     1801 tctcgaccgt cttcggggta ttgggtacgg cgttgttttt gtggctttta ttaaggaaac
     1861 ctgcccatgc cgtctgaaaa aaatatcggt tttatggcag gaagcagccg tccgttgtgg
     1921 gtagcttttg cattgttgct ggtttcctgc gtcctgttta tgacgctcaa cgtcaaaggc
     1981 gattgggatt ttgtactgca cctgcgtctg accaaacttg ccgcgctgct gatggtcgcc
     2041 tatgcggtcg gcgtgtccac gcaactcttc caaacgctga ccaacaatcc gattctgacc
     2101 ccttcaattt tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2161 ttcggcggcg tgggctatgc ttccctgccg ttgacgggca aattcggctt tgaacttgcc
     2221 gttatgatgg gcggctcgct gctgctgttt tacacgctca tccgtcaggg cggacgcgat
     2281 ttgccgcgta tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg
     2341 ctgctttcgc gcatgatcga ccccgaagaa tttacggcgg cgcaggcgaa tatgtttgcc
     2401 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgattct gctcgtcagc
     2461 gcggcggtcg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2521 gccgtcaatt tgggcatcag ctacacgcgc aacaccttgt ggatactgct ttggattgcc
     2581 gcattggtgg cgacggcgac cgccgtggtc ggcccggtaa gctttttcgg gcttctcgcc
     2641 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gccgatgacg
     2701 gtttgtgtcg gcggcatcct cctggtcggc ggacagaccg tattcgaaca cttcttgggc
     2761 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     2821 gttttaaaac acaaaaaatg acggatgcca tctgaacggc agcccgcccc gaaaggacaa
     2881 accatatgac gcaagaacgt ttacccgaat ttttcgaccg cgccccgacg ctgaccgtac
     2941 aagacccgct tgccgcattc ctcggcgcgg ccgaaaacgg catcctcact taccgctacg
     3001 ccgatgccgt gcgcctgtgc ggacattcct gcccgaccgt cgcgggcgcg tacctgatgg
     3061 ttatcaaagg tctgaaagca ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3121 cctttatgca gggcgcgcgc gacgaaggca cggtcggcgt aaccgcgtcc gtcgtccaac
     3181 tccttaccgg cgccgcaccc gaaaccggct ttggcggcgt aggccctgcc ggacgctttg
     3241 cccgccgcaa cctcttgtcc tttggtgcag gcgaaatcaa cggcacactc gcgctccgcc
     3301 gccgcgatac cggcaaaacc gtcgccgtca gcctcaacgc cgccctgcaa cccttcgcac
     3361 ccgaaatgcg cgaacttatg cccaaaaccg tcggcggcag cgcaagcgca gaagaactcg
     3421 aacgcttcgg acaactctgg caggcacgcg ttaaagcatt tttgaccgaa tcggcggacg
     3481 acccgcagtt cgtcatcgtc cgcgaagtgt gagcgttcag acggcattcc gaatttcaaa
     3541 tgccgtccga acccccgcca aacaacaaca aacctacgcc cgacaagcat ccgccatgat
     3601 taccatccgc aacgtcagct accgcatcgg cacacgcccc atcctcgaca acgtcagcct
     3661 cgacatcccc gaaggcggca ttaccgccct cgtcggcccc aacggtgcgg gcaaatccac
     3721 cctgttttcc tttatggcgc ggctgcgccc gcttgaaagc ggcagcatcg cctaccgcgg
     3781 caaaaacctt gcctgcaccc ccaccgccga actcgccaaa accctgtcca tcctcaccca
     3841 agaaaacagc atcatgagcc gcatcaccgt gcgcgacctg ctcatgttcg gccgctatcc
     3901 ctaccatcaa ggcagaccga ctgccgaatg ccgccgtatc gttaacggtg caatcgaaga
     3961 attccacctg caagacttcg ccgaccgcta cctgaccgag ctttccggcg gacaacgcca
     4021 acgcgccatg attgccatgg tgttctgcca gcgcaccgac tacgttttgc tggacgaacc
     4081 gctgaacaac ctcgacatgt atcatgcccg ctccctcatg caaatcctgc gccggctgac
     4141 cgacgaacac aagcgcacca ccgtcgtcgt attacacgac atcaaccagg ccgccgccta
     4201 cgccgaccac gtcgtcgcca tgaaaaacgg ccaagtcgcc atgcagggca agcccgacga
     4261 cattttcacc gccgcaaaca tcaaaaccct gttcgatatg gatgtgtccg tcctcgacca
     4321 ccaaagcaaa aaactcatcg tccaccatat ctgaagctca aaaagaaaat gccgtctgaa
     4381 aacctttcag acggcatttc cattccaaac gctcaaaccg ccgcgtccgc acgttcgccc
     4441 gtgcggatac ggattgcctc ctcgaccggc agcacaaaaa tcttgccgtc gccgattttt
     4501 cccgaacgcg ccacctcgac aatcacgtca atcgcgcgtt ccacagcgtc atccgccaac
     4561 accagctcga ttttgacctt gggcaggaaa tcgacagcgt attccgcgcc ccggtaaatc
     4621 tccgtatgcc ccttctgcct gccgaaccct ttgacctcgc tgacggtcat acccgtaatg
     4681 ccgatttccg tcaacgcctc gcgcacgtcg tcgagtttga acggtttgac aatcgcctcg
     4741 atttttttca tcacattctc cattaattaa cgtaaacaaa cgacaaatat agcagaaacc
     4801 gccggcaaac gataaacggt actgttcgcc cggcgatgcg gcgatttgcc ggaaaccgtc
     4861 attcccgcgc aggcgggaat cccgatctct gcatttcagc aatatttaaa ggtttctgta
     4921 aatccaaact tccatattcc taaccgtacg gaaacgacgg catttgatct aaaaacatac
     4981 ggaagcagtt gcccgcccct ctacttcctg caattatccg catacccggc tttgggtaaa
     5041 aatcagaaaa acagataaaa cccttattcc ttaatatctt cagacggcac ctgtattttc
     5101 cccaactgta tgccgtctga agattgtcaa cattttaagc caaaataccc aagcaataca
     5161 gccccgttgc gcgtataatg acagattttc caaccgcatt tgagagccga atccatgtct
     5221 gtcgttttgc ccttgcgcgg cgttaccgcc ctttccgatt tccgtgtcga aaaactcttg
     5281 caaaaagccg ccgcactcgg actgcccgaa gtcaaattaa gcagcgaatt ttggtatttc
     5341 gccggcagcg agaaagcatt ggatgccgcg actgtcgaaa aactgcaagc cttgttggcg
     5401 gcgcaaagcg ttgaacaaac gccaaaagcg cgcgagggct tgcatttgtt tttggtaacg
     5461 ccccgtttgg gtacgatttc gccgtgggct tccaaggcga ccaatatcgc ggaaaactgc
     5521 ggtttggcag gcatcgaacg catcgagcgc ggcatggcgg tgtggctgga aggtcgtctg
     5581 aacgacgatg agaaacagca atgggcggcg ttgctgcacg accgcatgac cgaatccgtg
     5641 ctgcccgatt ttcagacggc atccaaatta ttccaccatc tcgaatccga aaccttttcc
     5701 accgttgatg ttttaggcgg cggtaaagag gctttggtca aagccaatac cgaaatgggt
     5761 ttggcactct ccgccgacga aatcgattat ctggttgaaa actatcaggc tttgcagcgc
     5821 aatccgtccg atgttgaatt gatgatgttc gcgcaggcaa acagcgaaca ctgccgccac
     5881 aaaatcttca acgccgattt catcctcaac ggcgaaaaac aaccgaaatc cctgttcggc
     5941 atgatacgcg acacgcacaa cgcgcatccc gaaggtacgg tcgtcgctta taaagacaat
     6001 tcgtccgtaa tcgaaggcgc gaaaatcgag cgtttctatc cggatgcggc ggaaaaccaa
     6061 ggctaccgtt tccacgagga agacacgcat atcatcatga aagtggaaac gcacaaccac
     6121 ccgaccgcca tcgcgccgtt tgcgggcgcg gcaacgggcg cgggcggcga aatccgcgac
     6181 gaaggtgcga cgggcaaagg ttcgcgtccg aaagcaggct tgaccggctt taccgtgtcc
     6241 aacctcaaca tcccctgcct caaacagccg tgggaacaag attacggcaa gccggaacat
     6301 atttcctcgc cgctggacat tatgattgaa ggccccatcg gcggcgcggc gttcaacaac
     6361 gaattcggcc gccccaacct cttgggctac ttccgcacct ttgaagaaaa atttgacggt
     6421 caggttcgcg gctaccacaa accgattatg attgccggcg gtctgggcag cattcaggcg
     6481 cagcagacgc ataaagacga aatccccgaa ggcgcattgc tgattcaact gggcggcccg
     6541 ggcatgctta tcggcttggg cggcggcgcg gcttcttcga tggataccgg cacgaacgac
     6601 gcgtctttag acttcaattc cgtacaacgc ggcaaccccg aaatcgaacg ccgcgcgcag
     6661 gaagtcatcg accgctgctg gcagctcggc ggcaaaaacc cgattatctc catccacgac
     6721 gtgggcgcgg gcggtttgtc caacgccttc cccgaactcg tcaacgatgc cggacgcggc
     6781 gcggtattca agctgcgcga agtgccgttg gaagaacacg gtttaacgcc tttgcaaatc
     6841 tggtgtaacg aatcgcaaga gcgttatgtg ttgtcgattt tggaaaaaga tttggacacc
     6901 ttccgcgcca tctgcgaacg cgaacgctgc ccgtttgccg tggtcggcac ggcgaccgac
     6961 gacggccatt tgaaagtgcg cgacgatttg ttcgccaaca accccgtcga tttgccgctg
     7021 aacgtcttgc tcggcaaacc gcccaaaacc acgcgtaccg acaaaacggt tacgccgtcc
     7081 aaaaaaccgt ttcacgccga cgatatcgac atcaccgaag ccgcctaccg cgttttgcgc
     7141 ctgcctgccg tagccgccaa aaacttcctg attaccatcg gcgaccgcag cgtcggcggc
     7201 atgacccacc gcgaccaaat ggtcggcaaa taccaaaccc ccgtagccga ctgcgccgtt
     7261 accatgatgg gcttcaatac ctatcgcggc gaagcaatgt ctatgggcga aaaaccgacc
     7321 gttgccctgt ttgacgcgcc cgcctcgggc agaatgtgcg tcggcgaagc catcaccaac
     7381 atcgcggcgg tcaacatcgg cgacatcggc aatatcaaac tctccgccaa ctggatggcg
     7441 gcgtgcggca acgaaggcga agacgaaaaa ctctaccgca ccgtcgaagc ggtttccaaa
     7501 gcctgtcagg cattggattt gagcattccc gtgggcaaag acagcctgtc gatgaaaacc
     7561 gtatggcagg atggcgaaga gaaaaaatcc gtcgtttcgc cgttaagcct gattatctcc
     7621 gcgttcgcgc cggttcaaga cgtacgcaaa accgttacgc ccgaattgaa aaacgtcgag
     7681 gacagcgtat tgctgtttgt cgatttgggc ttcggcaaag cgcgtatggg cggctctgca
     7741 ttcggtcagg tatacaacaa catgagcggc gacgcgcccg atttggacga cacaggccgt
     7801 ctgaaagcgt tttacaacgt gattcaacag cttgtcgccg aagacaaact cttggcatac
     7861 cacgaccgca gcgacggcgg cttgtttgcc gttttggtag aaatggcgtt tgcagggcgg
     7921 tgcggcttgg atatagattt aaatttattg cttgcacaaa catttattac caaccatacc
     7981 gctctgtctc aatcattgcg gactgaagag gtaaaagcgt tggctgaatg gcaagaaacc
     8041 attgcccgca cattatttaa tgaagagttg ggtgctgtta tccaagttag aaaacaagat
     8101 gttgccgata ttatcaattt attctatcaa caacagctgc atcataatgt ctttgaaatc
     8161 ggtacgttaa ctgatgagaa cacgttaatc atccgcgacg ggcaaacgca ccttatttct
     8221 gacaacctaa tcaaactgca acaaacctgg caagaaacca gccatcaaat ccaacgcctg
     8281 cgcgacaacc ctgcctgcgc cgacagcgag tttgccctga ttggcgacaa cgaccgcagc
     8341 gcattgtttg ccaacctgaa attcgacgtg aacgaagaca tcgccgcgcc gtttatcaac
     8401 agcggcgcga aacccaaaat cgccatcctg cgcgaacagg gcgtgaacgg gcaaatcgaa
     8461 atggccgccg cgttcacccg tgccggattc gatgcctacg acgtgcatat gtccgacctg
     8521 atggcaggcc gcttccgcct tgccgacttc aaaatgctgg cggcgtgcgg cggcttcagc
     8581 tacggcgacg tactcggcgc gggcgaaggc tgggcgaaat ccatcctgtt ccaccctgct
     8641 ctgcgcgacc agtttgccgc cttcttcgcc gacccggaca cgctgacatt gggcgtgtgc
     8701 aacggctgcc aaatggtcag caacctcgcc gagattatcc cgggtgcaga ggcatggccg
     8761 aagttcaaac gtaacttgag cgaacagttc gaagcgcgcc tgagtatggt tcacgtcccc
     8821 aaatccgcgt cgctgattct gaacgaaatg caaggctcca gcctgcccgt cgtcgtcagc
     8881 cacggcgaag gccgcgccga cttcgcgctt cacggcggca acatttctgc tgatttgggc
     8941 atcgcgctgc aatatgtgga cggacaaaac caggttaccc aaacctatcc gctcaaccct
     9001 aacggctcgc cgcaaggcat cgccggtgtg accaacgccg acggccgcgt gaccattatg
     9061 atgccccacc ccgaacgcgt gtaccgtgcc gcgcaaatga gctggaaacc ggaaggctgg
     9121 acggaactgt ccggctggta ccgcctcttt gccggcgcac gtaaagcctt gggctaaccg
     9181 ccctactcaa accaatgccg cctgaagaat atttcagacg gcgttccggc ataccatcct
     9241 ttaaacggta tccgtccacc gaggaacact catgaaaatc acccccgtca aagccttgac
     9301 cgacaactac atctggatga tacagcacgg caaccatgcc gtctgcgtcg acccttccga
     9361 accctcgccc gtcttggaat tcctcgtccg caaccgcctc atgcttgccc aaacatgggt
     9421 aactcacccc catcccgacc acgagggcgg tgcggcggca ctctggcgcg gctacatgga
     9481 atcgcccgtt tacggcgaat ccgacatcga agcagcaacc cacaccgtaa ccgccggcac
     9541 ccaattcacc ttcggcgacg gacaggttac cgtttgggca acacccggcc acacagaccg
     9601 ccacaccagc taccttctcg aaacttcaga cggcatacac gtcttttgcg gcgacaccct
     9661 tttttccgcc ggctgcggac gcgtgtttac cggcacaatc gaacagcttt acgacagctt
     9721 ccaacgcttc aaccgcctgc ctgaaaacac cctgttctat ccggcgcacg aatacaccgc
     9781 cgccaacctg gattttgccg cccatatcga gccggacaac gccgacattc agacggcact
     9841 gaaagcggcg gcgcatacgc ctaccctgcc cgttaccctc gcgcacgaac gccgtgtcaa
     9901 tccgtttttg cgcgtcgacc tgccgcacgt cagagaccgc gccgaggcat tgagcgggaa
     9961 aacgttaaac agcagcctcg atacctttgt cgcgctgcgt gaacttaaaa accaataccg
    10021 gacgaaataa aacaacggga aaacgcagcc attcctagga tttttattaa aatcttaaat
    10081 aaaatcatac aatcatcgcc aatagacgaa aggacaccgt tgccttataa tcaaacaaaa
    10141 acaaaatata taatatagtg gattgaattt aaatcaggac aaggcgacga agccgcagac
    10201 aatacagata gtacggcaag gcgaggcaac gctgtactgg tttttgttaa tccactatat
    10261 aaatccagca caaaacggga tcggtgattc ttgtccgcaa gaatcgttga ttttctctat
    10321 tacacggata atcatcatgc gcttcacaca caccacccca ttttgttccg tattgtccac
    10381 cctcggtctt tttgccgttt cccctgctta ctcatccatt gtccgcaacg atgtcgatta
    10441 ccaatatttt cgcgactttg ccgaaaataa aggcgcgttc gccgtaggtg caagcaatat
    10501 ttccatccaa gacaagcaag gcaaaatatt aggcagggtt ctcaacggca tccccatgcc
    10561 cgacttccgc gtcagcaacc gccaaaccgc catcgccacc ctggttcacc cccaatacgt
    10621 caacagtgtc aaacacaacg tcggctacgg ttccatacaa ttcggcaacg acacccaaaa
    10681 tccagaagaa caagcctata cctaccgcct cgtatcacgc aacccgcacc cggactacga
    10741 ctaccacctt ccccgcctca acaaactggt taccgaaatc tcacctgccg cactcagcag
    10801 cgtacccttg cttggaaacg gccagccaaa ggccaatgcc tacctcgata ccgaccgctt
    10861 cccatacttt gtacgactcg gctcaggcac gcaacaagtc cgcaaagcag acggcacgcg
    10921 tacacgaacc gccccggcat accaatacct gaccggcggc acgccgctga aagtattggg
    10981 gttccaaaac cacggcttac tcgtcggcgg cagcctgacc gaccaacccc ttaacaccta
    11041 cgcaatcgcc ggagacagcg gttcccccct gtttgccttc gacaagcatg aaaaccgctg
    11101 ggtgcttgcg ggcgtactca gcacctacgc cggcttcgat aatttcttca acaaatacat
    11161 cgtcacgcaa cccgaattca tccgttccac catccgccaa tacgaaaccc ggctggatgt
    11221 cgggctgacc accaacgaac tcatatggcg cgacaacggt aatggcgaca gcaccctgca
    11281 agggctcaac gaacgcatca ccctgcccat tgcaaaccct tcgcttgccc cacaaaacga
    11341 cagcaggcac atgccgtctg aagatgccgg caaaacgctc atcctatcca gcaggttcga
    11401 caacaaaaca ctgatgctgg cagacaatat caaccaaggc gcaggcgcat tgcagttcga
    11461 cagcaacttc accgtcgtcg gtaaaaacca cacatggcaa ggtgcaggcg ttatcgtagc
    11521 cgacggcaaa cgcgtcttct ggcaagtcag caaccccaaa ggcgaccggc tctccaaact
    11581 gggcgcaggc acgcttatcg ccaacggaca aggcatcaac cagggcgaca tcagcatcgg
    11641 ggaaggcact gtcgtactcg cccaaaaagc tgcttcagac ggcagcaaac aagcattcaa
    11701 ccaagtcggc atcaccagcg gcaggggcac ggccgtcctc gccgacagcc agcaaatcaa
    11761 acccgaaaac ctctatttcg gcttcagggg cggacggctc gacctcaacg gcaacaacct
    11821 tgcctttacc catatccgcc atgcggacgg cggcgcgcag atcgtcaacc acaaccctga
    11881 ccaagccgcg acactgacgc tgaccggcaa ccccgtcctc agtcccgagc atgtcgagtg
    11941 ggtgcaatgg ggcaaccgtc cgcaaggcaa cgcggcggtt tacgaataca tcaacccgca
    12001 ccgcaaccgt cggaccgact acttcatact caaacccggc ggcaacccgc gcgaattttt
    12061 cccgttaaat atgaaaaact caacaagctg gcaatttatc ggcaacaaca ggcaacaggc
    12121 cgccaaacaa gtcgcccaag ccgaaaatgc ccgccccgac ctaattacct tcggcggata
    12181 cttgggtgag aacgcgcaaa cgggcaaagc cgcaccgagt tacagcaaaa ccaatatcga
    12241 atgggtgaat ggtccaaaca ccccacacaa gccctcgccc tatacgaata cgccaacccg
    12301 cacaggggca ggcgtaccga cgattaccgc ctcaaagaaa gcggcaaccc cggcgcctac
    12361 ttcccatgga atgcctacag cacccaagcc tgggaatttc tcggacatga ccggcgaagc
    12421 agccatagaa aaaacccgcc atatcgcaaa tgccgccgta tacggcaggc ccgaataccg
    12481 ttacaacggc gcactcaacc tgcactatcg tcccaaacgc accgacagca cgctgttgct
    12541 caacggcggc atgaacctta acggggaagt cttgattgag ggcggcaata tgattgtgtc
    12601 aggcaggccc gtaccccatg cctacgacca ccaggccaaa cgcgaacccg ttcttgaaaa
    12661 cgaatggacc gacggcagct tcaaggctgc acggttcacc ctgcgaaacc atgcccgact
    12721 gacggcaggg cgcaataccg cgcatctgga cggcgacata accgcatacg atctgtccgg
    12781 catcgacctc ggctttaccc aaggcaaaac accggaatgc taccgctcct accatagcgg
    12841 cagcacccac tgcacaccca acgccgtttt aaaagccgaa aactatcgtg cactacctgc
    12901 aacgcaagta cgcggcgaca ttacccttaa cgaccgttca gagctccgcc tgggcaaagc
    12961 acacctgtac ggcagcatcc gtgccggcaa agacaccgca gtccgcatgg aagcagacag
    13021 caactggaca ctttcccagt ccagccacac cggcgcactg acgcttgacg gcgcacaaat
    13081 taccctgaac cccgatttcg ccaataatac acacaacaac cgcttcaaca cactgaccgt
    13141 caacggcaca cttgacgggt tcggcacatt ccgattcctg accggcatcg tccgaaaaca
    13201 aaatgccccc cctcaaactg gaaggggaca gccgcggcgc attccaaatc cacgtcaaaa
    13261 acaccggaca agaacctcaa acaaccgaat cgcttgcact tgtgagcctc aatccgaaac
    13321 acagccacca agcccgattc accctccaaa acggctatgc cgatttgggt gcctaccgct
    13381 acatcctccg caaaaacaac aacggataca gcctgtacaa cccgctcaaa gaggccgaac
    13441 ttcaaattga agccacgcgt gcggaacatg agcgcaacca acaggcatac aaccaattac
    13501 aggcaaccga catcagcaga caggttcaac atgactctga cgcgaccagg caggcactac
    13561 aggcctggca gaacagtcaa accgaacttg cccgcatcga cagccaagtc caatatctgt
    13621 ccgcccaatt gaaacagaca gacccgctga ccggcattct gacgcgtgcc caaaacctgt
    13681 gtgccgcaca aggatacagt gtcgatatct gccgtcaggt tgccaaagcc gccgacacga
    13741 acgacctgac actcttcgaa accgaactgg atacgtatat agaacgtgta gaaatggccg
    13801 aatccgaact tgacaaagca cggcaaggcg gcgatgcgca agccgtcgaa acagcccggc
    13861 acgcctacct gaacgcactc aaccgtctgt cccgacaaat ccacagtttg aaaaccggcg
    13921 ttgccggcat ccgtatgccg aacctggccg aactgatcag ccggtcggcc aacaccgccg
    13981 tttccgaaca ggccgcctac aataccggcc ggcaacaggc gggacgccgc atcgaccgcc
    14041 accttaccga tccgcagcag caaaacatct ggctggaaac cggtacgcaa caaaccgact
    14101 accatagcgg cacacaccgt ccctaccaac aaactaccaa ctatgcacat atcggcatcc
    14161 aaaccggcat caccgaccgt ctcagtgtcg gtacgatttt aaccgatgag cgcacaaaca
    14221 accgttttga tgaaggcgta tccgcccgaa accgcagcaa cggcgcacat ctgttcgtca
    14281 aaggggaaaa cggcgcactc tttgccgcgg cagatttagg ctacagcaac agccgtaccc
    14341 gatttaccga ttatgacggg gctgccgtcc gccgccacgc atgggatgca ggcatcaaca
    14401 ccggcatcaa aatcgatacc ggcatcaacc tcagacccta tgccggcatc cgtataaacc
    14461 gcagcaacgg caaccggtac gtactcgacg gcgcagagat aaacagcccg gcgcaaatcc
    14521 aaaccacatg gcatgccggc atccgtctcg ataaaaccgt cgaactgggt caagccaagc
    14581 tgacccccgc tttcagcagc gattactacc atacccgcca aaacagcggt tccgccctca
    14641 gcgtcaacga ccgtacctta ctgcagcaag ccgcccacgg cacactgcat accctgcaaa
    14701 tcgacgccgg atacaaaggc tggaacgcca aacttcatgc cgcttacggc aaagacagca
    14761 acaccgcccg ccacaaacag gcaggaatca aaataggcta caactggtaa caagccggta
    14821 aaaatgccgt ctggaacccg cgtttcagac ggcatttgcg ttaaaaatag taaaccgttt
    14881 caaaagggag tagaatagtg ccgtttccaa ccctgcgcct gtaccgtcag gcttttatta
    14941 tggaccttcc cagttcgttt ttactgaacg ccccttccga ttccaaacga caataaccat
    15001 cccgccggaa tgcctccccg cacacggcgg gcggagcatt tatgagcatc gaacacactc
    15061 ctccgacaca cgacggcgaa accggtcaaa accatgccga acgcccttcc gccgatttcg
    15121 accgtgtcca ctccctctgc gaaatcctcg aacctgcttt tgaacaaatc gaaaacggca
    15181 caccgctcga agacgcgccg ctgcgcgaca agctgaccga gctgaccgtc ctcttgggcg
    15241 agctgcaccc tgccgacgtg gcggcggtgt tggaatcgct gccgccgcgc gaacgcaacg
    15301 tcgtttggct tctggtcaaa cccgaagacg acggcgaagt attgctggaa gtatccgacg
    15361 cggtgcgcga aacactgatc gagtcgatgg ataaggacga attgttggcg gcagttgacg
    15421 atttggacgc ggacgaattg gcggaactgg cagacgattt gccgcaccaa gtggtttacg
    15481 aagccttaca gacgcgtgat gaggaagaac gcgcccaagt caaggcggca atgtcctacg
    15541 aagacaacca agtcggtgcg attatggact tcgagttggt cagcatccgc gccgatgtcg
    15601 cctgcgaagt ggtgctgcgt tacctgcgcc gcttcgacag cctgcccgac cacacggata
    15661 aaatcttcgt ggttgacgaa aacgatgtgt tgcagggcgt gctgcccatc cgcaaacttt
    15721 tggtcgccga tcccgaagac ttggtggaaa acgtgatggc gaaagatgtc gtgcgtttcc
    15781 gcgccgaaga cgacgtggaa gaagcagcgc aggcgtttga acgctatgac ttggttaccg
    15841 cgcccgtcgt cgatgaaaac aaaaagctca tcggcagggt gaccgttgac gaaatggtgg
    15901 acgtgatccg cgaagaatcc gaagcagata tgttgaacat ggcgggtttg caagaagagg
    15961 aagacttatt cgcccccgtc ttggattccg tcaaaaaccg ctggatgtgg ctcgccgtca
    16021 acctctgcac cgccttcctt gccagccgcg tcatcggcgc gtttgaaggc agcatcgaaa
    16081 aaatcgtcgc actcgccgcg ctgatgccca tcgtcgccgg tatcggcggc aactcgggca
    16141 accagacgat taccatgatt gtccgcgcca tggcgatggg gcaactgaca ggcatgcagg
    16201 caggcaggct gttgaaaaaa gaagtcggcg ttgccctagt caacggcatt atctggggaa
    16261 ccgtcatggg cgcggtttca tggctgctct acggcagcct cggcatcggg ctggttatga
    16321 ttgccgcgat gacgctcaac ctcctgctgg cggcaaccgt cggcgtatta attcccgtgg
    16381 taatggaaaa gttcggacgc gatcccgcac tgggcagctc ggtgctgatt accgccgtta
    16441 ccgactccgg cggcttcctg attttcttgg gtctcgccac cctgttcctg ctttaaatgc
    16501 cgtctgaacc cgcgcaaaaa tgccgtctga agcggaagct gcttcagacg gcatttgact
    16561 atttatcctt gttgcacaag attattggac ggtatgccgg ggcagccctt tggcaacgcc
    16621 gaccacatcc tccccgaaca gcgcgttgac atcggtttcg tcaaacacat atttg
//
LOCUS       DAXHTR010000031        16187 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.031, whole genome shotgun sequence.
ACCESSION   DAXHTR010000031 DAXHTR010000000
VERSION     DAXHTR010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16187
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.031"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            49..124
                     /locus_tag="ACFNML_000705"
     tRNA            49..124
                     /locus_tag="ACFNML_000705"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Trp,seq:cca)
     gene            239..517
                     /gene="secE"
                     /locus_tag="ACFNML_000706"
     CDS             239..517
                     /gene="secE"
                     /locus_tag="ACFNML_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFC6392119.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            519..1055
                     /gene="nusG"
                     /locus_tag="ACFNML_000707"
     CDS             519..1055
                     /gene="nusG"
                     /locus_tag="ACFNML_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFC6392120.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPTPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            1156..1590
                     /gene="rplK"
                     /locus_tag="ACFNML_000708"
     CDS             1156..1590
                     /gene="rplK"
                     /locus_tag="ACFNML_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFC6392121.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQGMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKDPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            1590..2285
                     /gene="rplA"
                     /locus_tag="ACFNML_000709"
     CDS             1590..2285
                     /gene="rplA"
                     /locus_tag="ACFNML_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFC6392122.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            2515..3015
                     /gene="rplJ"
                     /locus_tag="ACFNML_000710"
     CDS             2515..3015
                     /gene="rplJ"
                     /locus_tag="ACFNML_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFC6392123.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            3073..3444
                     /gene="rplL"
                     /locus_tag="ACFNML_000711"
     CDS             3073..3444
                     /gene="rplL"
                     /locus_tag="ACFNML_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFC6392124.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            3635..7813
                     /gene="rpoB"
                     /locus_tag="ACFNML_000712"
     CDS             3635..7813
                     /gene="rpoB"
                     /locus_tag="ACFNML_000712"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFC6392125.1"
                     /translation="MSYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVADRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDKDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            7970..12145
                     /gene="rpoC"
                     /locus_tag="ACFNML_000713"
     CDS             7970..12145
                     /gene="rpoC"
                     /locus_tag="ACFNML_000713"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFC6392126.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVNQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGAAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIITDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMAANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQG
                     VEQETAETQVTDE"
     gene            12437..12694
                     /locus_tag="ACFNML_000714"
     CDS             12437..12694
                     /locus_tag="ACFNML_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392127.1"
                     /translation="MKPRNLFFAGCLLTSATFAEDIGVPVELINVGNRIAMPSEGESL
                     ALLPFAEDVPPVRDAMPSEVPKSAAGGDVRGNRMGMPINIG"
     gene            12691..12840
                     /locus_tag="ACFNML_000715"
     CDS             12691..12840
                     /locus_tag="ACFNML_000715"
                     /inference="COORDINATES: protein motif:HMM:NF016462.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6392128.1"
                     /translation="MSAALWHKKLSWKGFTPQINFRYNKINSNMPAFYSRSGKERFVS
                     IEKTY"
     gene            13019..13390
                     /gene="rpsL"
                     /locus_tag="ACFNML_000716"
     CDS             13019..13390
                     /gene="rpsL"
                     /locus_tag="ACFNML_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003093744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFC6392129.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            13508..13978
                     /gene="rpsG"
                     /locus_tag="ACFNML_000717"
     CDS             13508..13978
                     /gene="rpsG"
                     /locus_tag="ACFNML_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFC6392130.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            13997..16102
                     /gene="fusA"
                     /locus_tag="ACFNML_000718"
     CDS             13997..16102
                     /gene="fusA"
                     /locus_tag="ACFNML_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFC6392131.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
BASE COUNT         4301 a         3589 c         4190 g         4107 t
ORIGIN      
        1 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aagtttagag gccaatagct
       61 caattggtag agtatcggtc tccaaaaccg agggttgggg gttcgagacc ctcttggcct
      121 gccaaataaa aaattaaccg gccttgtgtc ggttaatttt tttgtatttg ttatttagta
      181 aactctcttg ccatttacat ggattgagaa tagacagatg ctatgatgga taaataatat
      241 gacagaacat acgcctgaaa aaaagaacgt taaagtggat caactggttg ttcaagataa
      301 agaatctgca tctaattccg gtaaggaagg gttttttgca tatttctcaa attcttggtc
      361 cgaattcaaa aaggtggttt ggcctaagcg tgaagatgct gtcagaatga ctgtatttgt
      421 tatagtgttt gttgctgtgc tttctatatt tatctatgcg gcagatacag caatttcgtg
      481 gttatttttt gatgtattgc tgagaaggga aggttgagat gtcgaaaaaa tggtatgttg
      541 tacaggcgta ttcggggttt gagaagaatg tccaacgaat attggaagag cgcattgccc
      601 gtgaggagat gggagattat ttcggacaaa ttctggtgcc tgtagagaaa gttgttgata
      661 tccgcaatgg tcgtaagact attagtgaaa gaaagtcata tcctggttat gtgctagttg
      721 agatggaaat gacagatgac tcttggcatc ttgtaaaaag caccccccgt gtttccggtt
      781 ttattggagg gagggctaat agacctacgc cgattagtca gagagaggct gaaattattt
      841 tacagcaggt tcagaccggc atagagaagc cgaaaccaaa agttgaattt gaggtcggtc
      901 aacaggttcg tgtaaatgaa gggccgtttg cggattttaa cggggtggtt gaggaggtca
      961 attatgaacg gaataagtta cgcgtgtctg ttcagatatt tggtagagaa acacccgttg
     1021 agctggagtt cagccaggtt gaaaagatta actgattttt atacttgaaa aaaaagcaat
     1081 aagaggatag aatcaaaaat taacttgggg agcggaaatg gttccgcgtc ttacccgttt
     1141 ttaggagttc gttaagtggc aaagaaaatt atcggctata ttaaactgca aattcctgca
     1201 ggtaaagcca atccatctcc tccggttggt cctgctttgg gtcagcgcgg tttgaatatt
     1261 atggaatttt gtaaggcatt taatgctgca acccaaggta tggagcctgg cttaccgatt
     1321 ccggttgtga ttactgcatt tgcagataaa tcattcacat ttgtgatgaa aaccccgcca
     1381 gcttctatct tgttgaaaaa ggctgccggt ttgcaaaaag gtagttctaa tcctctgacc
     1441 aacaaagtgg gtaaattgac ccgtgcccag ttggaagaaa ttgctaaaac taaagatcct
     1501 gatttgactg ctgctgactt ggatgcggct gtccgtacta tagcaggttc tgctcgctca
     1561 atgggcttgg atgtggaggg tgttgtataa tggctaaagt atctaaacgc ttgaaagctc
     1621 ttcgctcttc tgtggaagcc aataaattat atgcaattga tgaagcaatt gctttggtaa
     1681 aaaaagcagc gactgctaaa tttgacgagt ctgttgacgt atctttcaac ttgggcgttg
     1741 atccgcgtaa atctgaccaa gttatccgtg gttcggtcgt tctgcctaaa ggcaccggta
     1801 agacaacccg tgtggctgta tttactcaag gtgcaaatgc agaagctgct aaagaagctg
     1861 gtgcagatat cgtcggtttc gaagatttgg ctgctgaaat caaagcaggc aatctgaact
     1921 ttgacgttgt tattgcttct cccgatgcaa tgcgtattgt tggtcaattg ggtaccatct
     1981 tgggccctcg tggtctgatg ccaaacccta aagtaggtac ggttacccct aatgttgccg
     2041 aagcagtaaa aaatgcaaaa gcaggtcaag tgcagtaccg taccgacaag gcaggtattg
     2101 ttcatgcaac gattggtcgt gcttcattcg ctgaagctga tttgaaagag aactttgatg
     2161 cgttgctgga tgctatcgtt aaagccaagc ctgctgccgc taaaggtcag tatctgaaaa
     2221 aagttgctgt gtctagcacc atgggtttgg gtattcgcgt tgatacatca agcgtaaata
     2281 actaatctta aggaattttc aagcagtttg gttttctggg ctgcttgaat ttgggctact
     2341 taaaattaag tagatgtcca agaccgtagg gatcgtaaga tttaatcgta actgccctac
     2401 gcagacggta gtcctgaaac acattgcaag attgcttgta agatgtcttt ttaggttacc
     2461 gcgctggtgg gatatcgttt tggtatcctg tttataaaca gtgggaggta gaccttgagt
     2521 ctcaatattg aaaccaagaa agtggcggtc gaggaaatta gcgcggcaat tgctaatgct
     2581 caaaccctcg tagtcgctga atatcgcggt atcagtgttt ccagtatgac tgagcttcgt
     2641 gcgaatgcac gtaaagaagg cgtttatttg cgcgttctga aaaatacttt ggctcgtcgt
     2701 gcagtgcaag gtacttcatt tgcagaattg gccgatcaaa tggttggtcc gttggtttac
     2761 gctgcttctg aagatgctgt tgctgctgct aaagtgttgc accaattcgc gaaaaaagat
     2821 gacaaaattg tcgttaaagc cggttcttac aatggcgaag taatgaatgc tgctcaggtt
     2881 gctgagttgg cttctattcc gagccgcgaa gagctgttgt ccaaactgtt gttcgttatg
     2941 caagctcctg tatcgggctt tgcgcgcggt ttggctgctt tggcagagaa aaaagccggc
     3001 gaagaagccg cttaatcgat tttgtttctg ttaatcaatt attttttaat acaatatttg
     3061 gagtaaaata gcatggctat tactaaagaa gacattttgg aagcagttgg ttctttgacc
     3121 gtaatggaat tgaacgactt ggttaaagct tttgaagaaa aattcggtgt ttctgctgct
     3181 gctgttgcag ttgcaggtcc tgctggtgcc ggtgctgccg atgctgaaga aaaaaccgaa
     3241 tttgatgtcg ttttggcttc tgccggcgat caaaaagtcg gcgtgattaa agttgtccgt
     3301 gcaattaccg gtttgggtct gaaagaagct aaagacatcg ttgacggtgc acctaaaacc
     3361 attaaagagg gtgtttctaa agctgaagcc gaagacatcc aaaaacaact ggaagaagcc
     3421 ggcgctaaag tcgaaatcaa ataatttgat gcttcttatg aaggctggca gttttctgcc
     3481 agccttattt tgcttcttaa aataagcatt aagtattgtt tacatttatt tgcatagttt
     3541 ttatcaaatc attgcaaata aatgtaaaca tcagattgat gcgtgccgtt gctttcagac
     3601 ggcctattat tgaaaattac ttttcggagt gtatatgagc tattcgttta ccgagaaaaa
     3661 acgtatccgt aagagttttg caaagcggga aaatgttttg gaagttcctt tcctgctggc
     3721 aacccaaatt gattcttatg cgaagttttt gcagctggaa aatgcttttg acaaacgtac
     3781 cgatgacggt ctgcaggcgg catttaattc tattttcccg attgtgagcc ataacggtta
     3841 tgcgcgattg gagtttgtgc attacacatt gggcgagcct ttgttcgata ttcccgaatg
     3901 tcagttgcgc ggaatcactt atgcagcccc cttgcgcgcg cgtatccgtt tggtgatttt
     3961 ggataaggaa gcatctaaac cgacggtaaa agaagttcgt gaaaacgaag tgtatatggg
     4021 cgaaattccg ttgatgaccc cgagcggttc ttttgtgatt aacggcacag agcgtgtgat
     4081 tgtctcccag ttgcaccgtt cgcccggtgt attcttcgag catgacaaag gtaagacgca
     4141 ctcttccggc aaattgttat tctccgcccg catcattccc taccgtggtt catggttgga
     4201 ttttgaattt gatccgaaag atttgctgta tttccgtatc gaccgccgcc gtaaaatgcc
     4261 ggtaacgatt ttgttgaagg ctttgggcta caacaatgag caaatcttgg atattttcta
     4321 cgacaaagaa acgttctatt tgtcttcaaa cggtgttcaa accgatttgg tcgcagaccg
     4381 tctgaaaggc gaaactgcca aggtcgatat cttggataaa gaaggcaatg tattggttgc
     4441 caaaggtaag cgcattactg cgaaaaatat ccgtgatatt accaatgcag gcctgacccg
     4501 tttggatgta gaaccggaaa gcctgctggg caaagcattg gctgccgatc tgattgattc
     4561 ggaaaccggc gaggtattgg cttctgccaa tgatgaaatt acagaagagt tgttggccaa
     4621 atttgatatc aacggcgtaa aagaaattac gaccctttat atcaatgagc tggatcaggg
     4681 tgcttatatc tccaatacct tgcgtacgga tgagactgcc ggccggcagg cggctcgtgt
     4741 tgcgatttac cgtatgatgc gtccgggcga accgcccacc gaagaggcgg tcgagcaatt
     4801 gtttaaccgc ttgttcttca gtgaagacag ctacgatctg tcccgcgtag gccgtatgaa
     4861 atttaatacg cgcacatacg aacaaaaact gtccgaagcc caacaaaact cttggtacgg
     4921 ccgcctgctg aacgaaacgt ttgccggtgc tgccgacaaa ggcggttatg tcctgagcgt
     4981 cgaagatatt gtcgcctcga ttgcgacttt ggtcgagttg cgtaacggcc atggcgaagt
     5041 ggacgatatc gatcacttgg gcaaccgccg agtacgttcg gtaggcgagc tgactgaaaa
     5101 ccaattccgt agcggtttgg cccgtgtgga acgtgccgta aaagaacgtt tgaatcaggc
     5161 ggaatcagaa aacttgatgc cgcacgattt gattaatgca aaacctgttt ctgccgctat
     5221 taaagaattc ttcggctcca gccaattgag tcagtttatg gatcagacca accccttgtc
     5281 tgaagtaacc cataaacgcc gtgtatctgc attgggtccg ggcggtttga cccgcgaacg
     5341 tgcaggattt gaggtgcggg acgtgcatcc gacccactac ggtcgcgtat gtccgattga
     5401 aacgcctgaa ggtccgaaca tcggtttgat caactcattg tccgtttatg cgcgcaccaa
     5461 tgattacggt ttcttggaaa cgccttaccg ccgcgttatc gacggcaaag taactgaaga
     5521 aatcgactac ttgtctgcca tcgaagaagg ccgctatgtg attgcacagg cgaatgccga
     5581 tttggacaaa gacggcaatc tgattggcga cttggttact tgtcgtgaaa aaggcgaaac
     5641 cattatggca acgcctgacc gcgttcaata tatggacgtg gcaaccggtc aagtggtatc
     5701 cgttgcagca tccctgattc cgttcttgga acacgatgac gcgaaccgcg cattgatggg
     5761 tgcgaatatg caacgtcagg cagtgccttg cttgcgtcct gaaaaaccga tggtcggtac
     5821 cggtatcgag cgttccgttg ccgttgactc tgctactgca atcgttgccc gccgaggcgg
     5881 cgtggtcgag tatgtcgatg ccaaccgtgt tgtgattcgt gtccatgacg acgaagcgac
     5941 tgccggcgaa gtgggtgtcg atatttacaa cttggttaaa tttacccgtt ccaaccagtc
     6001 taccaatatc aatcagcgtc ctgccgtcaa agccggcgat gttttgcaac gcggcgattt
     6061 ggtggccgat ggcgcgtcca ccgatttggg cgaattggct ttgggtcaaa atatgaccat
     6121 cgccttcatg ccgtggaacg gttacaacta cgaagactcg attctgattt ccgaaaaagt
     6181 ggctgcggac gaccgctata cttcgattca cattgaggaa ttgaatgtcg ttgcccgcga
     6241 taccaagctg ggtgcggaag acattacccg cgatattccg aacttgtccg agcgtatgca
     6301 aaaccgtttg gacgaatccg gtatcgttta catcggtgcg gaagtagaag ccggcgatgt
     6361 gttggtaggc aaggtaacgc ctaaaggcga aacccagctg acgccggaag aaaaactgct
     6421 gcgcgccatc ttcggtgaaa aagcatctga cgtaaaagat acttcattgc gtatgcctac
     6481 cggcatgagc ggtaccgtta tcgacgttca agtcttcact cgtgaaggta ttcaacgcga
     6541 caaacgtgct caatccatta tcgattccga gttgaaacgc taccgtttgg atttgaacga
     6601 ccaattgcgt attttcgaca acgacgcatt cgaccgtatc gagcgtatga ttgtcggtca
     6661 gaaagccaac ggcggcccga tgaagctggc caaaggcagt gaaatcacga ccgaatatct
     6721 ggcgggtctg ccgagcaggc acgattggtt cgatatccgt ctgaccgatg aagatttggc
     6781 caagcagttg gaactgatta aagtgagcct gcaacaaaaa cgcgaagaag cggacgagtt
     6841 atacgaaatc aagaagaaaa aactgaccca aggcgacgaa ttgcaacccg gcgtacaaaa
     6901 aatggtgaaa gtttttatcg ccatcaaacg ccgtctgcaa gccggcgaca aaatggcggg
     6961 ccgccacggt aacaaaggcg tggtatcgcg cattctgcca gtggaagaca tgccttacat
     7021 ggcggacggc cgtccggtag acatcgtact gaacccattg ggcgtacctt cccgtatgaa
     7081 catcggtcag attttggaag ttcacttggg ttgggcagca aaaggtatcg gcgagcgtat
     7141 cgaccgtatg ctgaaagagc aacgcaaagc aggcgagttg cgcgagttct tgaacaaact
     7201 ctacaacggc agcggcaaga aagaagattt ggatgccctg actgatgaag aaatcatcga
     7261 actggcctcc aacctgcgca aaggtgcatc tttcgcctct cctgtattcg acggtgcgaa
     7321 agagtctgaa atccgcgaaa tgttgaactt ggcttatccg agcgacgatc ctgaggttga
     7381 aaaactgggc ttcaacgaca gtaaaaccca aatcacgctg tatgacggcc gttcaggcga
     7441 agcatttgac cgcaaggtta cagtaggtgt gatgcactat ctgaaactgc accacttggt
     7501 tgacgaaaaa atgcacgcgc gttctaccgg tccgtacagt ctggttaccc agcagccttt
     7561 gggcggtaaa gcccagttcg gcggccaacg tttcggtgag atggaggttt gggcactgga
     7621 agcatacggc gcagcctaca cgctgcaaga gatgctgact gtgaagtctg acgacgtgaa
     7681 cggccgtacc aaaatgtacg aaaacatcgt caaaggcgaa cacaaaatcg atgccggtat
     7741 gcccgagtcc ttcaacgtat tggtcaaaga gattcgctca ctgggcttgg atatcgattt
     7801 ggaacgttac taaacaaaag ttttcagacg gcctttcagg gtcgtctgaa aaagtggttt
     7861 cagaataaga atgaagcaat cggcatttag gccgtctgaa atcaaaagta ccgtttcccc
     7921 atatcgaaaa tccgccatgc ggtaaaaata cttccttcaa ggagcaaaaa tgaatttgtt
     7981 gaacttattt aatccgttgc aaactgccgg catggaagaa gagtttgatg ccattaaaat
     8041 cggtattgcc tctcccgaaa ccatccgctc atggtcttat ggcgaagtta aaaaacctga
     8101 aaccatcaac taccgtacgt tcaaacctga gcgtgacggt ttgttctgtg ccaaaatctt
     8161 tggcccggtc aaagactacg aatgcttgtg cggaaaatac aaacgcttga aatttaaagg
     8221 tgtaacttgt gaaaaatgcg gcgtggaagt aaccctgtcc aaagtgcgcc gcgaacgcat
     8281 gggccatatc gaattggctg cgcccgtcgc acatatttgg ttcttaaaat ccctgccttc
     8341 ccgcttgggt atggtgttag acatgacttt gcgcgatatc gaacgcgtat tgtactttga
     8401 agcatttgtg gtaaccgatc ccggtatgac tccgctgcaa cgccgccaat tgctgactga
     8461 agacgattac tacaacaagc tggacgaata cggcgacgac ttcgatgcca aaatgggtgc
     8521 agaaggtatc cgcgaattgc tgcgtacctt gaatgtagca ggcgaaatcg aaatcctgcg
     8581 ccaagagttg gaatcgaccg gttccgacac caaaatcaaa aaaatcgcca aacgcttgaa
     8641 agtattggaa gctttccatc gttccggtat gaagctggaa tggatgatta tggatgtgct
     8701 gccggtattg ccgcctgatt tgcgtccgtt ggttccattg gatggcggtc gttttgccac
     8761 ttccgatttg aacgatttgt accgtcgcgt catcaaccgt aacaaccgtc tgaaacgtct
     8821 gttggaactg catgcgcccg acattatcgt ccgcaacgaa aaacgtatgt tgcaagaagc
     8881 agttgactcg ctgttggata acggccgtcg cggtaaagcc atgaccggcg ccaacaaacg
     8941 cccgctgaaa tcattggcag acatgattaa aggtaaaggc ggtcgcttcc gtcaaaacct
     9001 gttgggcaaa cgtgtggact actctggccg ttccgtgatt accgtaggcc catacctgcg
     9061 tctgcaccaa tgcggtctgc cgaaaaaaat ggctttggaa ttgttcaaac cgttcatttt
     9121 ccacaaattg gaaaaacaag gtttggcttc tactgttaaa gctgcgaaaa aattggtaga
     9181 acaagaagta cctgaagtat gggatatctt ggaagaagtc atccgcgaac atccgattat
     9241 gttgaaccgt gcgccaaccc tgcaccgttt gggtattcaa gcgttcgaac ccatcctgat
     9301 tgaaggtaaa gccattcagt tgcatccgtt ggtatgtgcc gcatttaacg ccgactttga
     9361 cggcgaccaa atggcggtac acgttccatt gagcttggaa gcacaaatgg aagcacgcac
     9421 gctgatgctg gcttcaaaca acgtattgtc tcctgccaac ggcgaaccga ttatcgtacc
     9481 ttcccaagac atcgtattgg gtctgtacta catgacccgc gaccgtatca atgccaaagg
     9541 cgagggcagc ctgtttgccg atgtgaaaga agtgcatcgc gcataccata ccaaacaggt
     9601 cgagctgggt acgaaaatta ccgtacgtct gcgcgaatgg gtgaaaaacg aagcaggtga
     9661 gtttgagcct gtcgttaacc gttacgaaac aaccgtcggc cgtgcattgt tgagcgaaat
     9721 cctgccgaaa ggcctgccgt ttgaatatgt caacaaagcg ttgaagaaaa aagaaatttc
     9781 taaactgatt aacgcatcgt tccgcctgtg cggcttgcgc gatacggtta tctttgctga
     9841 ccacctgatg tacaccggtt tcggatttgc ggcaaaaggc ggtatttcca ttgccgttga
     9901 cgatatggaa attccaaaag aaaaagcggc cttgctggct gaagccaatg ccgaggttaa
     9961 agaaatcgaa gaccaatacc gtcaaggttt ggttaccaac ggcgaacgct acaacaaggt
    10021 ggtcgatatt tggggtcgtg ccggcgataa aatcgctaaa gcgatgatgg acaacctgtc
    10081 caaacagaaa gttatcgacc gtgacggcaa cgaagtcaat caagagtcat tcaactccat
    10141 ctatatgatg gcggactccg gtgcccgtgg ttctgcggct cagattaaac agttgtccgg
    10201 tatgcgcggg ttgatggcta aacctgacgg ctcgattatt gaaacgccga ttacctcaaa
    10261 cttccgcgaa ggtttgaccg tattgcaata ctttattgcg actcacggtg cgcgtaaagg
    10321 tttggcggat accgcgttaa aaaccgcgaa ctccggttac ctgacccgtc gtctggtaga
    10381 cgtaactcaa gatttggtcg ttgttgaaga cgattgcggt acttcagacg gctttgtcat
    10441 gaaggcagtg gtacaaggcg gtgatgtgat tgaagcattg cgcgatcgta ttttgggccg
    10501 tgttactgcg tctgacgttg tcgatccgtc aagcggcgag accttggttg aagccggtac
    10561 gttgttgact gaaaaactgg tggatatgat tgaccaatcc ggtgtcgatg aagtcaaagt
    10621 ccgtactccg attacttgta aaacccgcca cggtttgtgc gcgcactgtt atggtcgcga
    10681 cttggcgcgc ggtaagctgg ttaacgccgg tgaagcagtt ggtgtgattg ctgcacaatc
    10741 cattggtgaa ccgggtaccc agttgaccat gcgtacgttc cacattggtg gtgcggcatc
    10801 ccgtgcggca gcagccagcc aagtggaagc caaatccaac ggtacggcac gtttcagcag
    10861 ccagatgcgt tacgttgcca acaacaaagg tgagttggtt gtcatcggtc gctcttgcga
    10921 agtcgtgatt catgatgaca tcggccgtga acgcgaacgc cacaaagtac cttacggtgc
    10981 catcctgctg gtacaagacg gagcggccat taaagccggt caaaccttgg caacctggga
    11041 tccgcatacc cgtccgatga tcaccgaaca tgcaggtatg gtgaaatttg agaacgtgga
    11101 agagggtgtt accgttgcga aacaaactga cgatgtaacc ggtttgtcca ctctggtagt
    11161 gattgacggt aaacgtcgtt cctctagtgc ttctaaactg ctgcgtccga ctgtaaaatt
    11221 gttggatgaa aacggcgtgg aaatctgtat tcccggtacg tccactccgg tttcgatggc
    11281 attccccgtt ggtgcggtga ttaccgtacg cgaaggtcag gaaatcggta aaggcgacgt
    11341 attggcgcgt atcccgcaag cctcttccaa aacccgcgac attaccggtg gtctgccgcg
    11401 cgttgccgaa ttgtttgaag cacgcgtgcc gaaagatgcc ggtatgttgg cggaaattac
    11461 cggtaccgtt tccttcggca aagagaccaa aggcaagcaa cgtctgatta ttaccgacgt
    11521 ggacggcgta gcatacgaga ctttgatttc caaagagaaa cagattttgg tacacgacgg
    11581 tcaagtggta aaccgcggcg aaaccattgt ggacggcgcg gtcgatccgc acgatattct
    11641 gcgtctgcaa ggtatcgaag cactggcacg ctacattgtc caagaggtgc aagaggttta
    11701 ccgtctgcaa ggtgtgaaga tttctgataa acacatcgaa gtcatcatcc gtcaaatgtt
    11761 gcgccgtgtg aacattgcgg atgccggcga aaccggcttc attaccggag agcaggtcga
    11821 acgcggcgat gtgatggcgg ccaatgaaaa agctttggaa gaaggcaaag aaccggcgcg
    11881 ttacgaaaac gtattgctgg gtattaccaa agcttccctg tctaccgaca gcttcatttc
    11941 tgctgcatcg ttccaagaaa cgacccgtgt tctgaccgaa gccgcgatta tgggcaaaca
    12001 agacgaactg cgcggtttaa aagagaacgt catcgtcggc cgcctgattc ctgccggtac
    12061 cggtttgact tatcaccgca gccgtcatca gcaatggcaa ggggtagagc aggagactgc
    12121 tgaaacccaa gtaacggatg aataatcttc ggcgcgttta ttcaatacaa aaccgcaagc
    12181 tttaagcttg cggtttttct ttgtccgatt aaggcaaaaa caagcgtttt cgtcattttg
    12241 aggcgtgtgg attattcctt aggtattttc gggccggaga ccaacgaggt ggcgggtgtc
    12301 gtcggtacgt ccggagacca aaataacttt gccagggatg ttggtttcgg cggtcaaaaa
    12361 aagtagcgtc ttaatgtttt ccatttaaac aaatgtcgtc tgaaacttca gacggcattt
    12421 cctttaaaaa ataaatatga aacccagaaa tctctttttt gcaggctgcc tgctgacttc
    12481 ggcgacgttt gccgaggata tcggcgtacc tgtcgaactg attaacgtcg gtaatcggat
    12541 tgcgatgccg tctgaagggg aaagcctcgc cctcctgccg tttgccgagg atgtaccgcc
    12601 ggttcgcgat gcaatgccgt ctgaagttcc taaaagcgcg gcaggcggcg atgttcgggg
    12661 taaccggatg ggaatgccga ttaacatcgg atgagcgcgg ctttatggca taaaaaactg
    12721 tcgtggaaag gatttacacc ccaaataaat ttccgttaca acaagatcaa cagcaatatg
    12781 cccgcctttt attcgcgcag cggcaaggaa cggtttgtca gtatagaaaa aacgtattga
    12841 cagtattttc ttcagtcgtc cgactgattg tgagggatgt tggtaaatat ttatcggcaa
    12901 acaagaaaat catctttctt cttgtcgtta tgcttgactg tatacttgca ataaaaatat
    12961 aattccactc ttgccgacat ggtgtcggca agtatttaac tcaacaggac gagaaaatat
    13021 gccaactatc aaccaattgg tacgcaaagg ccgtcaaaag cccgtgtacg taaacaaagt
    13081 gcccgcactg gaagcctgcc cgcaaaaacg cggcgtgtgc acccgtgtat acacgactac
    13141 ccctaaaaaa cctaactctg cattgcgtaa agtatgtaaa gtccgcctga ccaacggttt
    13201 tgaagtcatt tcatacatcg gcggcgaagg ccacaacctg caagagcaca gtgtcgtatt
    13261 gattcgcggc ggtcgtgtaa aagacttgcc aggtgtgcgt taccacactg tacgcggttc
    13321 tttggatact gcaggtgtta aagaccgtaa acaagcccgt tccaaatatg gtgctaagcg
    13381 tcctaaataa ttactgggac ttaaataggc acgtcggccg cctaagcaga acaacggccg
    13441 agtaagtgaa tactcaattg ggtattcatg ggaatagacc cgactgaata gattaaagga
    13501 aattaaaatg ccaagacgta gagaagtccc caagcgcgac gtactgccag atcctaaatt
    13561 cggcagcgtc gagttgacca aattcatgaa cgtattgatg attgacggta aaaaatccgt
    13621 tgccgagcgt atcgtttacg gtgcgttgga acagattgag aaaaaaaccg gcaaagtagc
    13681 aatcgaagta tttaacgaag ccattgcaaa cgccaaacct atcgtggaag tgaaaagccg
    13741 ccgtgtaggt ggtgcaaact accaagttcc tgttgaagtt cgtccttcac gccgtttggc
    13801 tttggcaatg cgctgggttc gcgatgcggc ccgcaaacgt ggtgagaaat ccatggacct
    13861 gcgtttggca ggcgaattga ttgatgcgtc cgaaggccgt ggcggtgcgt tgaaaaaacg
    13921 tgaagaagta caccgtatgg ctgaagccaa caaagcattc tctcacttcc gtttctaatt
    13981 ttgaaaggct aataaaatgg ctcgtaagac cccgatcagc ctgtaccgta acatcggtat
    14041 ttccgcccat atcgatgcgg gtaaaaccac gacgacagaa cgtattttgt tctataccgg
    14101 tttgacccac aagctgggcg aagtgcatga cggtgcggct actaccgact acatggaaca
    14161 agagcaagag cgcggtatta ccattacctc cgctgccgtt acttcctact ggtccggtat
    14221 ggcgaaacaa ttccccgagc accgcttcaa catcatcgac accccgggac acgttgactt
    14281 taccgtagag gtagagcgtt ctatgcgtgt attggacggc gcggtaatgg tttactgcgc
    14341 ggtgggcggt gttcaacccc aatctgaaac cgtatggcgg caagccaaca aataccaagt
    14401 gccgcgcttg gcgtttgtca ataaaatgga ccgtcagggt gccaacttct tccgcgttgt
    14461 cgagcaaatg aaaacccgtt tgcgcgcaaa ccctgtacct atcgtcattc cggttggtgc
    14521 ggaagacaac ttcagcggtg tggttgattt gttgaaaatg aaatccatca tttggaatga
    14581 agccgataaa ggtacaacct ttacctatgg cgatattcct gccgaattgg tcgaaactgc
    14641 cgaagaatgg cgtcaaaata tgattgaagc cgcagccgaa gccagcgaag aactgatgga
    14701 caaatactta ggcggcgacg agctgaccga agaagaaatc gtaggcgcgt tgcgtcaacg
    14761 tactttggca ggcgaaattc agcctatgct gtgtggttct gcatttaaaa acaaaggtgt
    14821 tcaacgtatg ttggacgcag ttgtagaatt gctgccagct cctaccgata ttcctccggt
    14881 tcaaggtgtc aacccgaata ccgaggaagc cgacagccgt caagccagcg atgaagagaa
    14941 attctctgca ttggcgttca aaatgttgaa cgacaaatac gtcggtcagc tgacctttat
    15001 ccgcgtttac tcaggcgtag taaaatccgg cgataccgta ttgaactccg taaaaggcac
    15061 tcgcgaacgt atcggtcgtt tggtacaaat gactgccgca gaccgtactg aaatcgaaga
    15121 agtacgcgcc ggcgacatcg cagccgctat tggtctgaaa gacgttacta ccggtgaaac
    15181 cttgtgtgcg gaaagcgcgc cgattatctt ggaacgtatg gaattccccg agccggtaat
    15241 ccatattgcc gttgagccga aaaccaaagc cgaccaagag aaaatgggta tcgccctgaa
    15301 ccgcttggct aaagaagacc cttctttccg tgtccgtaca gacgaagaat ccggtcaaac
    15361 cattatttcc ggtatgggtg agctgcactt ggaaattatt gttgaccgta tgaaacgcga
    15421 attcggtgtg gaagcaaata tcggtgcgcc tcaagtggct taccgtgaaa ctatccgcaa
    15481 agccgttaaa gccgaataca aacatgcaaa acaatccggt ggtaaaggtc aatacggtca
    15541 cgttgtgatt gaaatggaac ctatggaacc gggtggtgaa ggttacgagt ttatcgatga
    15601 aattaaaggt ggtgtgattc ctcgcgaatt tattccgtct gtcgataaag gtatccgcga
    15661 tacgttgcct aacggtatcg ttgccggcta tcctgtagtt gacgtacgta tccgtctggt
    15721 attcggttct taccatgatg tcgactcttc ccaattggca tttgaattgg ctgcttctca
    15781 agcgtttaaa gaaggtatgc gtcaagcatc tcccgccctg cttgagccga ttatggcagt
    15841 tgaagtggaa accccggaag aatacatggg cgacgtaatg ggcgacttga accgccgtcg
    15901 cggtgttgta ttgggtatgg atgatgacgg tatcggcggt aaaaaagtcc gtgccgaagt
    15961 acctctggca gaaatgttcg gttactcgac cgacctgcgt tctgcaaccc aaggccgcgc
    16021 tacttactct atggagttca agaaatattc tgaagctcct gctcacatag ctgctgctgt
    16081 aactgaagcc cgtaaaggct aatcaggcaa ataggccgtc cgaaaggctg aaatgatttt
    16141 tcagacggca ttgttcttta atcgatcttt aatgtaaagg aattagc
//
LOCUS       DAXHTR010000032        16175 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.032, whole genome shotgun sequence.
ACCESSION   DAXHTR010000032 DAXHTR010000000
VERSION     DAXHTR010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.032"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(45..1226)
                     /gene="odhB"
                     /locus_tag="ACFNML_000719"
     CDS             complement(45..1226)
                     /gene="odhB"
                     /locus_tag="ACFNML_000719"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFC6392132.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAAPAEAAPAAAPAA
                     AQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAAAAPAVALPAG
                     ARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKYKEKFEKEHGV
                     KLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRGLVVPILRDAD
                     QMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTPIINPPQSAIL
                     GMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDALEDPARLLLD
                     L"
     gene            complement(1326..4154)
                     /locus_tag="ACFNML_000720"
     CDS             complement(1326..4154)
                     /locus_tag="ACFNML_000720"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFC6392133.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDTVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPERVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVVGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            complement(4359..5642)
                     /gene="gltA"
                     /locus_tag="ACFNML_000721"
     CDS             complement(4359..5642)
                     /gene="gltA"
                     /locus_tag="ACFNML_000721"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFC6392134.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPEQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYCLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            complement(5754..6002)
                     /locus_tag="ACFNML_000722"
     CDS             complement(5754..6002)
                     /locus_tag="ACFNML_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HFC6392135.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            complement(6006..6713)
                     /locus_tag="ACFNML_000723"
     CDS             complement(6006..6713)
                     /locus_tag="ACFNML_000723"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (quinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HFC6392136.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITSERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="ACFNML_000724"
     CDS             complement(6834..8597)
                     /gene="sdhA"
                     /locus_tag="ACFNML_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFC6392137.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPADAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="ACFNML_000725"
     CDS             complement(8600..8941)
                     /gene="sdhD"
                     /locus_tag="ACFNML_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HFC6392138.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFSQTWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="ACFNML_000726"
     CDS             complement(8935..9312)
                     /gene="sdhC"
                     /locus_tag="ACFNML_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HFC6392139.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            complement(9591..10994)
                     /locus_tag="ACFNML_000727"
     CDS             complement(9591..10994)
                     /locus_tag="ACFNML_000727"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6392140.1"
                     /translation="MKKIQADVVVLGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDSFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAS
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            complement(11243..11980)
                     /locus_tag="ACFNML_000728"
     CDS             complement(11243..11980)
                     /locus_tag="ACFNML_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HFC6392141.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LQFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            complement(12259..14535)
                     /gene="metE"
                     /locus_tag="ACFNML_000729"
     CDS             complement(12259..14535)
                     /gene="metE"
                     /locus_tag="ACFNML_000729"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFC6392142.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQVAANADFVAVGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTGFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALAVDLPKEWV
                     EAYKDVYATLSKVNAKILLSTYFGSVAEHAALLKALPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGVIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKNQELRVLKAALNEGRDSVAEELAASQAAADSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARSAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRATVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTRKLRAELAK"
     gene            complement(14673..15551)
                     /gene="metF"
                     /locus_tag="ACFNML_000730"
     CDS             complement(14673..15551)
                     /gene="metF"
                     /locus_tag="ACFNML_000730"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFC6392143.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPAGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            15751..16026
                     /locus_tag="ACFNML_000731"
     CDS             15751..16026
                     /locus_tag="ACFNML_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFC6392144.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            16026..16151
                     /gene="ykgO"
                     /locus_tag="ACFNML_000732"
     CDS             16026..16151
                     /gene="ykgO"
                     /locus_tag="ACFNML_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFC6392145.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRRGKVYVICKSNPRFKARQR"
BASE COUNT         3980 a         4091 c         4022 g         4082 t
ORIGIN      
        1 tcagacggcc ttttcattaa caaataaaag gccgtctgaa acgattacag atccaacaac
       61 aggcgggctg ggtcttccaa cgcgtcttta atggctacca aggtcaatac agcttcgcgg
      121 ccgtcaatga tacggtggtc gtaagacaga gccagataca tcattggacg gacaacaact
      181 tggccgtttt caaccacagc gcgctcttta gtggcgtgca tacccaaaat cgcagattga
      241 ggcggattga tgatcggggt ggacatcata gaaccgaaag taccgccgtt ggtaatactg
      301 aatgtaccgc cggtcagatc ttcgatagcg attttgccgt ctttggcttt tttcgcgtaa
      361 tcaacaattg cttgttcgat gtcggcaatg ctcatttggt cggcatcgcg caggattggc
      421 acaaccaaac cgcgtgggct gccaatcgcg ataccgatgt cgaagtagcc gtggtacaca
      481 atgtctttgc cgtcaacaga agcattcaca accgggtatt ttttcagggc tgcaacagcg
      541 gctttaacga agaaggacat aaagcccagt ttcacgccgt gttctttctc gaatttttct
      601 ttgtacttcg cacgcaagtc catgattggt ttcatgttga cttcgttgaa tgtagtcaga
      661 atggcgtttt cttgttgaga agccaagagg cgttctgcaa cacgggcacg caggcggctc
      721 atcggtacgc gttcttcagg acgtgcgccg gcaggaagtg caacggcagg agcagcggct
      781 gcggcaggtt tggctgcggc attttgtacg tcttctttca atacgcgacc gtcacggccg
      841 gaaccttgca atgcgttcac gtcaacaccg ctctctgcag ccagtttggc ggcggcaggc
      901 atagcggcgt tgttttgtgc agcagcagga gcggcggccg gggcagcttc ggcaggagcg
      961 gcggcaggtg cttcagcagc ggcagtagca gcggtatcga tacgtgccaa aacttggtcg
     1021 gcaacaacgg tttcaccgtc ttgcgctacg atttcaacca atacgccggc ttgtggagaa
     1081 ggtacttcca aaaccacttt gtccgtttcg atatcgatca ggatttcgtc acgggcaact
     1141 gcttcgccaa cttttttctt ccattccaag agcgtgcctt cagatacgct ttcagacagc
     1201 ataggtactt ttacatcaat aatcattttg tgtctccaat ggccctttca gacggcctgt
     1261 tgtgtgtttt tcattctgca tatgggcatt ccgcctgagc agcctcagac ggaatgctgc
     1321 ttggtttaca acgccaaagc gtcttcaacc aattgtttca attgagcaat gtgtttgctt
     1381 gagtagccca ctgcaggcga tgcgctgctt ggacgaccgg cataagacag tttttgctct
     1441 tcgctgataa cgtcttcgat gcggtggcgg atttggtaga acgcgccttg gtttttcggc
     1501 tcttcttgcg cccaaaccac agattttgcg ttcggatatt tcgccagttc ggctttaacc
     1561 tcatcgtatg ggaacggata cagctgctca acgcggacaa tcgcaacatc atcttccagt
     1621 ttacgttcgg ctcgaccggc ttccaagtca tagtaaacct gaccggcaca caataccacg
     1681 cgtttcacgc tgtcgttgct tgcgcgttcg gcggtatcgc cgacaaccgg acggaaagtc
     1741 gaaccttcgg tgaagttttc cagcgggctc attgcccctt tgaatcgcaa caggcgtttg
     1801 gacatgaaaa ttaccagcgg tttgcggtat gaacctaaga cttgacgttg caagaggtgg
     1861 aacatttgcg acgcttcaga tggcatgatg acttgcatat tgttctcaga acacagttgc
     1921 aaccagcgtt ctacgcgtgc agaagagtgc tcagggcctt gaccgtcgta accgtgcggc
     1981 aggatggtgg tcagaccgca caagcgaccc catttggttt cgcctgaaga caggaattgg
     2041 tcaatagtca cttgcgcgcc gttggcgaag tcaccgaatt gagcttccca aatggtgagc
     2101 ttgtcaggag cggagcaggc aaagccgtac tcgaacgcca tcacggcctc ttcgttcaag
     2161 atggagtcga taaccaggaa ctcgcccatt ccttcgccca tatggcgcag gggaacataa
     2221 gtaccgtcgt cccatttttc gcgtttttga tcgtgcaata cggcgtggcg gtgcgagaac
     2281 gtaccgcggc cggagtcctc accggagata cgcacgccat gacctttggt tacgaggctg
     2341 gcgtatgcca aggtttcggc cataccccaa tctatggcct gtttgccgga tgccatggct
     2401 ttacgcgctt caatcacacg ttttgcagtc ggatgcaggg caaagccttc cggtacggcg
     2461 gtaaacttct cagtgagacg ctcaatatct gcggcaggca aaccggtttc gatgtgttcg
     2521 cgccaatctt tgccttggta tttgctccag tcgatttgcg tgcgttgaaa gttgctcaac
     2581 gttgtttgct cgacgtgttc gcctttgtcc aaagcatcac ggtaggcttg gatgtaaccg
     2641 tctgcctcgg cttgggttac cacgccttcg gcaatcagtt gctcggtgta caaagcacgc
     2701 gcaccggggt gttgcgatac ttttttgtac atcatcggtt gggtcaaggt cggatcatcg
     2761 ccctcgttgt gaccccattt acggtagcag acaacgtcaa tcacgatgtc tttatggaat
     2821 tttttgcggt aatccaaagc ggcttggatg gcaaagcaaa cacgctcagg atcatcgccg
     2881 ttcacatgga taaccggagc ggaaaccatt tttgcgatat cggtacagtg tacggttgaa
     2941 cgggtatcgc ggatatcgga agtggtaaag ccgatttgat tgttgatgac gatatgaacc
     3001 gtaccgccgg tggtataacc gcgcgtttta gacaggttga atgtcgcttg gttgacaccc
     3061 aaaccgataa atgcggagtc gccgtgaatc aatacgggca agactttatc gcggccgttt
     3121 tcgcccaaac gtttttgctt ggcgcgtgca gaaccttcca ctaccggatt gacgatttcc
     3181 aagtgggacg ggttgaacgc caaagaaaca tgcatcgggc cgtgcggggt agcaatatcg
     3241 gagctgaagc ccatatggta tttcacgtcg ccgctgggca gtttgatttc ggcacgacct
     3301 tcaaattcgg caaacaaatc gccgggtttt ttgcccaaaa tgttcaccaa aacattcaga
     3361 cggccacggt gcgccatacc gatgatgact tcttccacgc cgtctttacc ggcgttttga
     3421 atcaggtagt tcaaaccggc aatcgcgctt tcgccgcctt cgacaccgaa acgtttctga
     3481 ccgacatatt tggtatgcag ataacgttcc aaagtctcgg cagcagtcat ctctttcaag
     3541 atacggcgtt tttgatcggc attgtaatgc ggtgtggaca atacgctttc aaaataattg
     3601 cgtacccagc ggcgctcttc ggtattggga atatagatat attccaatgc gatgtggccg
     3661 cagtaggttt gtttgaggtt gctgatgatt tgggacaaag gcagtttgcc gcgattggca
     3721 aaatcgccct cgcccatatt gaattgaagc gccatatcgg catcagacag accgtggaat
     3781 ttcggatcga gggcttcaat atcgcgcggg gggatacgtt tgagcggatc aagttgggct
     3841 gcacccacgc cttggatacg ataggcagaa atcagccgta aaacgctgac ttgctttttc
     3901 agcattgcct catccgcacc gcccgcaacg gcagatgcaa ttttcttttt cgccaaagta
     3961 acaaatgatt cgcgaatcgg tgtgtgtgcg acatcgacag caaccgtccc cggctgtttg
     4021 ctcaaatcgg tgaaatactg cttccatttt tcatcaaccg tatcggggtt ttccaaaaaa
     4081 gcctcgtaca attcctcaat gtagggtgcg ttcgaaccga acaggtaaga gaaattgagt
     4141 ttttcgtcca tcatggcgtg cgctcttttc tttaaaatat aaacaaaggg tagaagtccg
     4201 cgtcctgacg gctaaactga tgccgtctga aaagacattt tattctaccc tattttaaac
     4261 cggattgctt gtcttgattt catccgacta caatcagacg gattcgggaa acaatccggt
     4321 tctctgatat tgcctgtttg acaatatatt aatgtatgtt acctctcgcc tggcggcaca
     4381 taatcgcggc gttccgaacc ggtataaagc tggcgcgggc ggccgatttt cagcgaagga
     4441 tcgctaatca tctcgtgcca gtgcgaaatc cagcccacgc tgcgcgacag ggcgaagatg
     4501 acggtaaaca tttcggtcgg gatgcccagc gcggacagga cgatgccgga atagaaatcg
     4561 acgtttggat acagtttgcg ttcgataaag aacgggtctt tcagcgcaat ctgttccaat
     4621 tccatcgcca gtttgaattt cggactgtct tccaagccca attccttcaa aacttcatag
     4681 caggtttcgc gcataatgct ggcacgcgga tccatattgc ggtacacgcg gtgaccgaag
     4741 cccatcagac agtatttgcg ttgtttcaca ccttccatgt atgcggcaac attagacaca
     4801 tcgccgattt cgtccaacat tttcaacacg gcttcgttcg caccgccgtg cgaagcaccc
     4861 cacaggcagg cgataccggc agcaatacag gcaaacggat tcgcacccga agagcctgcc
     4921 agacggacgg ttgaagttga ggcgttttgc tcgtggtcgg catgcaaaat aaagatgcgg
     4981 tcgagcgcgc gtgccaaaac gggattgggt ttgtagtctt cacacggcgt ggcgaacatc
     5041 atatgaagga agttttcgga ataagaaaga ttattcttcg gataattgaa cggcagaccg
     5101 tttgaatagc ggtagcacat tgccgcaatg gtcgggattt tagaaatcag gcggtaaatc
     5161 gcgattttgc ggtgttcggg attgctaatg tccaagctgt cttggtagaa cgcagacagt
     5221 gcgccgacca cgccgaccat catcgccatc ggatgcgcgt cgcggcggaa gccccggaag
     5281 aaccaagtca gctgttcatg caccatcgtg tggcggcgga cggtattgtc aaattctgcc
     5341 ttttgctcgg gagtcggcag ttcgccgtaa atcaacaggt agcagacttc caaataatcg
     5401 gacttttcgg ccagctgctc gatggggtat ccgcgataat aaagcaagcc ttgatcgccg
     5461 tcgatgtaag taattttaga ctcacagctt gcggttgaaa caaatccggg gtcgaaggaa
     5521 aacaaacctg tattttttgt cagcccccga atgtcaacca catcgtgccc gatgctggct
     5581 tccaataccg gcagctccaa acctgcctga cccggtacgt tgagtttgat tgatttggac
     5641 atattattgc tcctttgcaa gttataaccc acgttactgc atccgcagca cgttctttgc
     5701 cttcagacgg cttttgcatt ttgaaatctg cctgcaggca gatttcagat gcctcatgcc
     5761 cgtctgattt tttcaagcat cgggataagg tgccctttgt ccgtttccga atgcccgtta
     5821 atcaaggcaa gcaattcttg atcttgaaat tcaaggattt cggaaaactc ggacagctct
     5881 ttatcgctca aatgctcgaa ttctttttcc ataaacctgc cgaagattaa atctaactcc
     5941 aacaatcccc ggcgggtttg aaaacggatt ttccgtttgg caatatcgtc aaaaaccatc
     6001 atttcttaaa cggcccgttt caacataatc tctttaatct taccgatggc tcgggtcgga
     6061 ttcaagtgtt tgggacatac gtctacgcag ttcataatgg tgtggcaacg gaacaaacgg
     6121 tatgggtcgt tcagattatc caaacgctca ctagtgatgg tatctcggct gtccgcaatg
     6181 aagcggtaag cattcagcaa accggacgga ccaacgaatt tatcagggtt ccaccaaaat
     6241 gacgggcagg cagtcgaaca gcaggcgcac aaaatacact cgtacaaacc gtccaactct
     6301 ttacgctctt cctgagtttg cagacgctct ttgtccgcat caatcggatt atcgttgaca
     6361 acataaggtt tgacggaatg gtattgtttg aagaactggg tcatatccac aatcaggtca
     6421 cggataacag gcagacctgg cagaggacga attttaaccg gctgtttcaa gccacgcaga
     6481 tcggtcaaac acgccaagcc gtttttgccg ttgatgttca taccgtcaga accgcaaatg
     6541 ccttcgcggc aggagcggcg gaaagacaag gtatcgtctt gtgctttcag gcgtaccaaa
     6601 gcatccaaaa gtttcacgtc ggtcggttcc aattccaact cgtaacgctg catataaggc
     6661 ttggcatcaa catccgggtt gtaacggtaa atttcaaaac tcattttttc catgagaagt
     6721 gttccttttc tcatcaactg cccaatgatt ttgtattata aattcaagca gttcaatgtg
     6781 ggtatggtta gatttcagac gagcttatga ggcgaaaacc gtctgaaaac gcatcaataa
     6841 acgcgcttgg ccggtttgat gtattccacg ctcaaaggct tggtatgtac cggtttgtaa
     6901 gacaaagtat tggtatctga atggtacagc gtatgtttca tccagttttc atcatcgcgc
     6961 tcaggatggt cgtctgaagc gtgcgcaccg cgtgattctt tacgtgcttc ggcagacacc
     7021 aaagtcgctt tcgccacttc aattaggtta tccaattcca aagcctcgat acgcgcggta
     7081 ttccacactt tgctcttgtc tttgatttcg gtacgtttca cacgctcggc aatcgccatg
     7141 acttctcgaa cgcctttgct cagaatctca tcagtacgga acacgccggc gtgcagttgt
     7201 acggagcgtt gcaattcgcg acgcaatgca tcaacgtttt cgccatcagt ttgattgtcc
     7261 aaacgctcga tacgttggcg ggtcagctcg cccgcatccg caggcaaagg tttccagtcg
     7321 ctttgctctt tgatgaattt aatcatgctg tcgccggcag ctttaccgaa taccaccaag
     7381 tccaacaggg agttggtacc caagcggttc gcaccgtgta cggaagcaca agcgcactca
     7441 cctgccgcat acagaccttt tacaggcact tcgtattcgt cgccttgagg aacgacaact
     7501 tcgccatggt agttggtcgg aataccgccc atcatatagt gggtagtcgg cacaacggga
     7561 atcgggtctt taatcggatc gataccggcg aactgaatgg aaatctcgcg gatgcccggc
     7621 agtttttcca taattttttc tgcgccgata tggtcgattt tcagtaagac atggtctttg
     7681 tttttaccgc agccgcgacc ttcgtagatt tccatcgcca tcgcgcggga aacaacgtcg
     7741 cgagaagcca agtcttttac ggtcggcgca tagcgttcca taaagcgttc gccgtcggca
     7801 ttcaacagaa taccgccctc gccgcgtacg ccttcggtaa tcaacacgcc cgcacctgcc
     7861 acgccggtcg ggtggaattg ccagaattcc atgtcttcca acgggatacc tgcacgcgcg
     7921 caaataccca aaccatcgcc ggtattcata taggcattgg tagaagaggc ataaatacgg
     7981 ccgccgccgc cggtagcaaa catcacagct ttagcgtgga aaatataaac ttcgccggtt
     8041 tccatttcca tggcggttac gccgacgaca tcgccgtttt catcacgaat caaatcttgt
     8101 gccgtccatt ccacaaagaa ttgcgtattg gcacggacgt tttgttggta caaagtatgc
     8161 agcatcgcat gacctgtacg gtcggcaacc gcacaggcgc gttctaccgc gcgtttaccg
     8221 tgttcggcag tatggccgcc gaaaggacgc tgataaattt taccgctttc cacacggtca
     8281 aaaggcatac ccatgtgttc caactcaatc accgcttcag gcgcagcgcg gcacataaac
     8341 tcaatcgcat cttggtcgcc cagccagtcg gaacctttca cggtatcgta catgtgccag
     8401 tcccaacggt cctcctgcac attacccaaa gaggcggaaa taccgccctg cgccgctacg
     8461 gtatgcgaac gggtcgggaa cactttagac aaaacggcac aattcagacc ggatttggat
     8521 aattggaggg ctgcgcgtaa acctgcacca ccaccgccga caatcacggc atcaaacttg
     8581 cgaacaggaa aacccatact tacccccaaa ttactttaat tgaatacacc aagcagccga
     8641 ccagccagac gatggtggca acctgcaaaa acaaacgcac gccgaagggt ttgatatagt
     8701 ccatccacaa atcgcggata cccacccaag cgtgcaagaa tacggcgatg aagctcactt
     8761 gggtaaatac ttttacccaa gtttgactaa aaaatgcctg ccatgccgaa tattctttag
     8821 gcagggaaaa tagaaccact aaaagtgcaa cggtataaat caacataata accgcagtcg
     8881 cacgttgcat cgcccaatcg cgcaaaccgt aatgggcacc ggtcaattta cgttctacca
     8941 taacaacgct cccaaaacga cagtcaaaac caatgcagaa gcaaatacgg ctttagcggt
     9001 attgcgcgca gtattcagct caaggccttt gtgcgcatcc aaaaataaaa agcggatacc
     9061 ggcgagagaa tggtgcagat aagcccacaa tacaccgatt aaaaccagct tgaccaaagg
     9121 atgggaaaca atggcacggt aagtttcaaa tgcagactct tgactcaggg taccggacag
     9181 gaaatacagc aggaaaggca gcataataaa cagcccgacc ccactgatgc ggtgaaggat
     9241 ggaaactatc ccgggtatcg gcagacggat gttcggcaaa tccagataaa caggacgcgg
     9301 tttggcagac atagtcaatt cctctgtagt tatatatcga ctcaaaaacc gtaaacccat
     9361 tgctacactg ggttacataa acgttaattt acctgtttta tcccattttg aacagtgtat
     9421 tttaaaaaca aactgataga ttttgacaaa cacgcgtgga aacagcagat taaaagaaag
     9481 atttttagtt ttgccgcccc cttcccgaca cggtatcagc atccgacccc atccccaaaa
     9541 acaaaatgcc gtctgaaaaa atttcagacg gcattgtttt gccatatcgg tcaggctttc
     9601 aatttcgcat cggcatcgcg caacgcggta cgcagacctt cctcgataac gggatggtag
     9661 aacggcatat ccagcatttg cggaacggtc atcttcattt gatgcgccca tgccaacaag
     9721 tgcgccaaat gttcggcggc agggcctacg atttccgcgc cgataaagcg gccggtggct
     9781 ttttcggcat acaggcgcat atggcctttg tttaccagca tcacgcggct gcggccttgg
     9841 tttttgaacg atacttcgcc gatgacaaat tcgtcggctt ggtattgcgc ggcaacctgc
     9901 gcgtatttca gaccgacaaa gccgatttgc ggactggtaa acaccacgcc gatggtgctg
     9961 cggcgcaaac cgccgccgat attcgggtag cggctcgcgt tatcgccggc aatcttgcct
    10021 tggtcggcag cttcatgcag cagaggcagt tggttggacg catcgcctgc gatgaagata
    10081 tgcggaatac tggtctgcat ggtcagcgga tcagcaacag gtacgccgcg cgcatctttt
    10141 tcgatattga tattttccaa accgatattg tcaacgttcg gacggcggcc cacggctgcc
    10201 agcatatatt cggcaacaaa tacgcctttt tcgccatcct gctcccaatg gacttctaca
    10261 ttgccgtctg catcgagctt gacctcggtt ttagcatcca aatgcagttt caattcttcg
    10321 ccgaacacgg ctttcgcctc gtctgaaacg acggggtcgg aaatgccgcc gatgattccg
    10381 cccaaaccga aaatttcaac tttcacgccc aaacggtgca atgcctgacc cagttccaaa
    10441 ccgataacac ccggcccgaa cacggcaacg cgcttaggca gcgtatccca tgagaaaacg
    10501 tcatcgttga taatcaaacg attgcccaaa gactgccatt gcggcaggat gacgggacgc
    10561 gaaccggtag caatcacgaa actttttgcc gtaatttgag tatgctcgtc gatttggacg
    10621 gtatgctcgt cgataaattt agccgaaccc atgatgcgct tgtcggcagg ccactcttcc
    10681 acatcggcaa cgacaaagcc gacaaaacgg tcacgctcgg atttaacgcg ctgcatgacc
    10741 tcttcaccgt tgacgacgat gctgtctttg tccaaatgca cgccgaacga gtcggtatgc
    10801 aatgcgtggt ggcgcgcttc tgcggcggca atcaagagtt tggaaggcat acaacccacg
    10861 cgcgcgcagg ttgtgccgaa cacattgttt tcaatcaggt aaacattatc cgaatgtaaa
    10921 cgggcattgc gaaacgcacc cataccggca gtaccgccgc cgagtacgac gacatccgct
    10981 tgaatttttt tcataatctt tgtccttccg ttttcgtctg aaacattgcc gacacagaac
    11041 atatcggttt cagacggcat ttaaatattt tcccaatcaa aaacatcatt cagataaata
    11101 aaaacaaaat ttcgattgag tgcaacaata tccaaattct cttataaata tagagttatt
    11161 cggagcagca gtgcagaacc gcccgaataa ctctatccgg tatatcagac agtttaatca
    11221 aaccgcctta agcaaacagc tatcagtttt tagccaagta agcttccaaa tcttcgctgc
    11281 cgccgatgta tttgccgccg atgaagactt gaggggcagt catcttgccg gtaatggcgc
    11341 gaacggaagt aacggttgca tctttgccca atacgatttc ttcgtaagac aaacctttgt
    11401 cttgcaaagc ttgtttggct ttagcgcaga attggcaacc tggtttagtg aaaattgcca
    11461 cagactcttg agccttccaa tcgggagcaa cgaattgcag catagtatct gcatcggata
    11521 ctttgaacgg atcgcccggt tcttcaggtt cgatgaacat tttttcaacc acgccgtcgt
    11581 taaccagcat ggagtaacgc caagagcgtt taccgaagcc caagtcttct ttaccgacca
    11641 gcatacccat accttcggta aattcgccgt tgccatcagg aatcatgtag atgttgtcgg
    11701 attcttcttc ggcagcccaa gcgttcatta cgaacgtatc gtttacagat acgcagtaga
    11761 ttgcgtcaac gccgttttct ttgaacgcgc cgaacaattc gttgtaacgc ggcaggtgtg
    11821 aagaagaaca agtcggggta aatgcaccgg gcagggagaa tacgactact tttttgcctt
    11881 tgaacaaatc atcggtagac acatctttcc aagtgtcgcc gacgcgggtg cggaatacta
    11941 cggaaggtac tttttgaccg gtacgatctt gcaaagccat tttaagctcc ttaatagatt
    12001 ggtttgagaa ataattgtga atcgctgggc attctacccc atgcaaaatg atttgtataa
    12061 tttatagttc aaataatata aattatttaa aactatcaaa cacaactccg atttgcctgt
    12121 ttcccacata taaagccgaa tgagaaaaaa acaagctgca atgaaaagat tttccggcaa
    12181 ctgcgccctt gcgccgcaaa tcaggacaaa ataccgtctg aaaggctttc agacggtatt
    12241 tattcatacg gtctcggctt atttcgccaa ttcggcacgc agttttcggg ttacgttcat
    12301 catcacttgg agttgttcca gagtttcttt ccagccacgt gttttcaggc cgcagtcagg
    12361 attcacccac agacgctcaa ccggtacaac ctcgatggct ttgcgcaaca ggtgctcaac
    12421 ttcggcttca gtcggtacgc gcgggctgtg gatgtcgtaa acccccgggc cgatgtcgtt
    12481 cgggtatttg aattcgccga acgcggtcaa gagttccata tcggaacgtg aagtttcgat
    12541 ggtgatgacg tcagcatcca tagcggcgat ggccggcaag atgtcgttga actcggagta
    12601 acacatatgg gtgtggatct gagtgctgtc ttcacaaccg gcagaggaca ggcggaaaga
    12661 ttcgcccgcc cagttcaggt aggcatccca atcggcacgt ttcaaaggca gaccttcgcg
    12721 gatggcaggt tcgtcgattt ggatgacttt gatgcctgct ttttccagat ccaatacttc
    12781 gtcgttcaga gccagtgcga tttgtttgca cacggtagcg cggggaatat cgttgcggac
    12841 gaaagaccat tgcagaatgg ttacagggcc ggtcaacata cctttcatcg ggcgtttggt
    12901 caggctttga gcgtaagtag accaagccac ggtcatggct tcaggacggc ttacgtcgcc
    12961 aaagataatc ggtggtttca cgcagcgtga gccgtagctt tgtacccagc cgtattgagt
    13021 gaatgcaaaa ccgctcaaca attcgccgaa gtattcaacc atgtcgttac gctcggcttc
    13081 gccgtgtacc agtacgtcca agtccagttt ttcttgctct tcaaccacca aggcgatttc
    13141 ttttttcatc gcggcttcgt aatcggcggc agacagttcg ccttttttga aggctgagcg
    13201 tgcctggcgg atttcggtgg tttgcgggaa agaaccgatg ttggtcgtcg gcagcagagg
    13261 taggttcaac catgcttgtt gcgctttgat acggtcggca aatggagatt tgcgttggtc
    13321 tgcgttggca ggcaaatcgg ccaggcgttt ggcaacgtct gcacgatgga tttcgctgct
    13381 gttggcacgg gagtcggcag cagcttggct ggcggcgagt tcttcggcaa cagaatcacg
    13441 gccttcgttc aatgcagctt tcagaacgcg caattcttgg tttttttgca gggtgaatgc
    13501 caaccaagag tacaggtcgg gtttgttggc tttcagtttt tcttcaactg acaagtcaaa
    13561 tggagtgtgc agcagcgagc aagagctgga aatccacaaa cggtcaccca gtttggcttg
    13621 cagaggctcg acagtttcca aaactttgtt caggttggcg cgccaaatgt tgcggccgtc
    13681 aatcacgccg gcagacagga ctttgtcgta gtcggcgaac gcgtccagtt gctcgggggc
    13741 gcgtaccaag tcgatgtgca gaccgtcaac aggcagggct ttcaacaatg cggcgtgttc
    13801 ggcaacagaa ccgaagtaag tgctcaacag gattttggcg ttgactttgc tcaaagtagc
    13861 gtaaacgtct ttgtaggctt ccacccattc tttaggcaaa tcgacagcca aagcaggctc
    13921 gtcgatttga atccactcgg caccggcttc aaccaaagca gtcaggattt caacgtaaac
    13981 aggcaacagt ttaggcaaca ggctcagacg gtcgaattcg acggcgcctt tttctttacc
    14041 cacccacagg aaagtcaacg gacctacaac ggtcggtttg gctttcagac cgagggcttg
    14101 ggcttcttgc agttgttgaa catagtgttt ggcattggct ttgaatccgg tatcggcgtg
    14161 gaattcaggc accaagtagt ggtagttggt gtcgaaccat ttggtcattt cgatagcgaa
    14221 ttggtctttg ttaccgcgcg ccagttggaa gaattgttcc aaagacaggt tttggctgtc
    14281 gaagccgaag cgggcgggaa tcgcgccggt ggcgacttgc aggtcgagga tgtggtcgta
    14341 gaaagtgaaa tcgcctacgg caacgaaatc ggcgttggca gcgacctggt gtttccagtt
    14401 tttctcgcgc aagtctttag caacagccag caattcttgc tcgctgattt ctttgcgcca
    14461 gtatttttct tgtgcgaatt tcaattcgcg gaaggcgccg acacgcggga agcctgagaa
    14521 atgtaatgtt gtcatgttga actctccagt tggattttgt aaggaccggt tcaatccgaa
    14581 tcggccggta ttgtaaatca gaatggctcg gatggggcgg tgttttgctt tttagacacc
    14641 ttagatgccg tctgaaactg tttgatacgg ctttaagggc gcacgcctaa aatatggcaa
    14701 atggcgtaag tcagctcgct gcggttaagc gtatagaagt ggaaatcttt cacgccttcg
    14761 cgggacagga ctttgaccat atcgatggcg atacttgccg ccaccagatt gcgcgtacct
    14821 tggtcgtcgt ccaaaccttc atacatttgc gacagccagc ttgggatttt gacgttggtt
    14881 acttgagcca ttttaccgag ctgcttgaag ttggtaacag gcaaaatacc ggggacgatt
    14941 tccacatcga tacccaacat cacgcagcgg tcgcggaagc gcaggtagcg ttccacatcg
    15001 aagaagaatt gggtgatgac gtggttcgcg cccgcatcga ttttgcgttt caaattaatc
    15061 aggtcggctt gtgcggattt cgcttcggga tgcacttcgg gatatgctgc tacagagatg
    15121 tcgaagtcgg cgacggagcg taatagctta accaagtctt cggcgtaaaa cggttttttc
    15181 tcataaccgg ccggctcgtc tccgcgcagg gcgacaatgc ggcggatgcc gctgtcccaa
    15241 taatctttgg caatttggcg caattcgtcg ggagaagcgt cgataccggt caggtgaggc
    15301 gcggcttcca agccggtttc ctgtttgatg cgtttgacga tgccgtgcgt gcggtcgcgt
    15361 tcgccggaat ttgcgccgta agttacggat acgaacttgg gatgcagggt ttgcagacgg
    15421 tggatggagt cccacagcat cgtttccatt tgttcgtttt tcggcgggaa aaattcaaac
    15481 gaaacgttaa tgtcgccttt caaatcagaa agactgttat ttaacgcatt gatttctttt
    15541 gcgtaattca tggctgcgta ccttttatta tcttttatat gatgtttcag gcaacataat
    15601 aaagttgcgc caaacatgag tcaatttcaa aattttcaga tttatcatga aaatttctaa
    15661 tatattacaa aaatagttaa atttccttgt acagcccttc taaactatgc tagagtttgt
    15721 tatgttataa cactgaaagc aaggcaatat atgaaaccaa atattcatcc tgacaattat
    15781 cgaaccgtcc tttttttcga cagcagcgcc aatgaaggct ggttaatccg ctcctgtgcc
    15841 gaaacacacg gaaaaacgat ggtttggaca gacggcaaag aatatccgct gttttcgctg
    15901 gatacttctt ccgcatccca tcccgtctat accggcaagc agcgtaacgt caacaccgaa
    15961 ggacgcgcca gcaagttcaa ccaacgtttc caatccgtca tgtcatcgtt tagaaaggac
    16021 aaataatgca ggttctgtct tcactcaaaa ccgccaaaca acgccaccgc gactgccaaa
    16081 tcgtcagacg caggggcaaa gtttacgtca tctgcaaaag caatccgcgt tttaaagcgc
    16141 gccaacgctg actccctgcc cttcctgctt cggtt
//
LOCUS       DAXHTR010000033        16047 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.033, whole genome shotgun sequence.
ACCESSION   DAXHTR010000033 DAXHTR010000000
VERSION     DAXHTR010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16047
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.033"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..79)
                     /locus_tag="ACFNML_000733"
     CDS             complement(<1..79)
                     /locus_tag="ACFNML_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6392146.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFL"
     gene            282..1610
                     /gene="miaB"
                     /locus_tag="ACFNML_000734"
     CDS             282..1610
                     /gene="miaB"
                     /locus_tag="ACFNML_000734"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFC6392147.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLPDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPDMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="ACFNML_000735"
     CDS             complement(1695..3608)
                     /gene="dxs"
                     /locus_tag="ACFNML_000735"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFC6392148.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRTFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMELAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVANLPKESAAQMPSEKEPKPAAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAEKLNATVADMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDDLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="ACFNML_000736"
     CDS             complement(3693..4610)
                     /gene="xerC"
                     /locus_tag="ACFNML_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFC6392149.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEDEGGVPQDLSRRDFTAALRRLSQRGLNARTLARKLSSWRQYCVWLVERGLLHTDP
                     TADIKPPKQPERVPKALSQEWLNRMLDLPVDGGDPLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLVGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4804..5868
                     /gene="fba"
                     /locus_tag="ACFNML_000737"
     CDS             4804..5868
                     /gene="fba"
                     /locus_tag="ACFNML_000737"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="catalyzes the reversible aldol condensation of
                     dihydroxyacetonephosphate and glyceraldehyde 3-phosphate
                     in the Calvin cycle, glycolysis, and/or gluconeogenesis;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFC6392150.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLMEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMASRYAKGELNQIVK"
     gene            6026..6847
                     /locus_tag="ACFNML_000738"
     CDS             6026..6847
                     /locus_tag="ACFNML_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H-binding protein"
                     /protein_id="HFC6392151.1"
                     /translation="MNRTAFCCLSLTAALILTACSSGGGGVAADIGAGLADALTTPLD
                     HKDKSLQSLTLDQSVRKNEKLKLAAQGAEKTYGNGDSLNTGKLKNDKVSRFDFIRQIE
                     VDGQTITLASGEFQIYKQNHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAF
                     NQLPDGKAEYHGKAFSSDDPNGRLHYSIDFTKKQGYGRIEHLKTPEQNVELASAELKA
                     DEKSHAVILGDTRYGGEEKGTYHLALFGDRAQEIAGSATVKIREKVHEIGIAGKQ"
     gene            6986..7663
                     /gene="tsaB"
                     /locus_tag="ACFNML_000739"
     CDS             6986..7663
                     /gene="tsaB"
                     /locus_tag="ACFNML_000739"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFC6392152.1"
                     /translation="MQADFNRPVLAVDTGTSYLSLALRADGETLLFHQEAGSRQSELI
                     LPEIRTLFRNAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFNTPLIGIPTLD
                     AAASLPPPQSCILAAADARMGEVFYAWFDTLNRRRLSDYQVGRAADIRLPEGCAFSDG
                     IGSAFALEETPPFSGKPDMPTAADFLNLAAEGGFPAVAAAHSELLYVRNKIALTAKEQ
                     AERKART"
     gene            7660..8100
                     /gene="rimI"
                     /locus_tag="ACFNML_000740"
     CDS             7660..8100
                     /gene="rimI"
                     /locus_tag="ACFNML_000740"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFC6392153.1"
                     /translation="MNIRRASPSDCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATAPECRRRGVASALLEYWFAHLPEDTQRLLLE
                     VRAGNTAAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8094..8831
                     /locus_tag="ACFNML_000741"
     CDS             8094..8831
                     /locus_tag="ACFNML_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6392154.1"
                     /translation="MLSARYLHLHEALGLGPMWLKQAAAVLPPKNTPATPAQARPQTV
                     RAAPIRPSQPHNGQARLETMKALETAAVHTRKPAPETETPPPGLSDGIAPVPAASGIT
                     KLAVVSLCPPIEDAVYGQLFYGKAGVLLDNILKAVGLDAAYVHKTCWVKTAAVGNPMP
                     SEAAIETATVSVMQELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARL
                     LRQPELKARAWQVLKQLKRALRQGGGS"
     gene            8893..9534
                     /gene="pyrE"
                     /locus_tag="ACFNML_000742"
     CDS             8893..9534
                     /gene="pyrE"
                     /locus_tag="ACFNML_000742"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFC6392155.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRRSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIRFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9608..10042
                     /locus_tag="ACFNML_000743"
     CDS             9608..10042
                     /locus_tag="ACFNML_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HFC6392156.1"
                     /translation="MPACFCPHCKTRLWVKENQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTRAIGKKQISRDEIAGILNGGTTQPDIPPATAATP
                     AAAPQVTVPPAAPARQDGFNWTIATLFALIVLIMQLSYLVIL"
     gene            10039..11349
                     /gene="argA"
                     /locus_tag="ACFNML_000744"
     CDS             10039..11349
                     /gene="argA"
                     /locus_tag="ACFNML_000744"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFC6392157.1"
                     /translation="MIVPDLFVAHFREAAPYIRQMRGKTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGARHFLDRHAAAQGRTPHYCRGLRVTDETSLEQAQQFAGTV
                     RSRFEAALCGSVSGFARAPSVPLVSGNFLTARPIGVIDGTDMEYAGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYSGKTFHLDMLQTAASVAVSLQAEKLVYLTLSDGISRPDGTL
                     AVTLSAQEAQSLAEHAGGETRRLISSAVAALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCATLKTFTEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEDELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11445..13460
                     /locus_tag="ACFNML_000745"
     CDS             11445..13460
                     /locus_tag="ACFNML_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family protein"
                     /protein_id="HFC6392158.1"
                     /translation="MKSYPDPYRHFEDLDSAETQNFAAEANAETRARFLNNDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPNRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWGERQLTESGYPREVWLVERGKSFEESLPVYQIDKGAMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSSEGEAKPLNLPNDCDVAGYLAGHLLL
                     TLRKDWHRANQSYPSGALVAVKLNRGELGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEVELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLALFAL
                     DLNVMELTVMRRQPQQFDSDGINVRQFWAVSSDGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSVGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLITAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGSSWTDEYGNPQKYEVCKRRLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSAQSWLYSPDGGGHTGNGTQRESADELACVLLFLKEFLG"
     gene            complement(13574..14530)
                     /locus_tag="ACFNML_000746"
     CDS             complement(13574..14530)
                     /locus_tag="ACFNML_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6392159.1"
                     /translation="MNTAAIYRQYQTYVRSDKSGWALDGCSDSALIAQAKQPGLHLEM
                     CINRFDSGITLSRMRGGGTGAFPTEIHNFSHNCALFVMVSGQNRLQMGGREYRPSAGE
                     IWLVRGELADVSETLLPDNSGMCALHLDFSLEKLRRWHDEGLLDERLFSPQTIGRFAL
                     QRLAQNAAALTAAACPLLQRPFESDGFGLLADEAAALELSARLLRFTFRRHDNGYRRR
                     RIDEAADILQHEFARPLTIAEIARRVGLNECYLKRYFKAQTGETVAGCLRRLRLEHAL
                     AFIESGSTVQAAMHFCGYRHAGRLNEAFRRHYGFLPSDAKKC"
     gene            14766..15731
                     /locus_tag="ACFNML_000747"
     CDS             14766..15731
                     /locus_tag="ACFNML_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6392160.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT         3567 a         4899 c         4386 g         3195 t
ORIGIN      
        1 gcaggaactg atgttccctg tcgccgaaat tgctggtaca cgcacacagc agcaatgccg
       61 cccacacagc cggtttcata cacacctccc attaaagcca aacattatat agccgtcccg
      121 accgattaaa ttcatatttt aaaacaatat tctgtttaca aaacccacat cgtgctataa
      181 tccgcaccga ttttcagacg gcatcgtcat gccgtctgaa attttttcat tccaacaaca
      241 atcagccccg cgattacggc tgcctgagaa agacacaaac catgaaaaaa gtatttatcc
      301 gcaccttcgg ctgccagatg aacgaatacg acagcgacaa aatgctcgcc gtcctcgccg
      361 aagaacacgg cggcatcgaa caggttaccc aagccgacga agccgacatc atcttgttca
      421 acacctgctc cgtgcgcgaa aaagcgcaag aaaaagtctt ctccgatttg gggcgcgtgc
      481 gtccgctcaa agaaaaaaac cccggcctca tcatcggcgt tgccggctgc gtcgcctcgc
      541 aagaaggcga aaacatcatc aaacgcgcgc cttatgtgga cgtggttttc ggcccgcaaa
      601 cgctgcaccg cctgccaaaa atgattgtgg acaaagaaac cagcgggctg tcgcaagtcg
      661 atatttcctt ccccgaaatc gaaaaattcg accacctgcc gcccgcccgc gtcgaaggcg
      721 gcgcggcatt tgtatcgatt atggaaggct gttccaaata ctgctccttc tgcgtcgtcc
      781 cctacacgcg cggcgaagaa ttctcccgcc cgctcaacga cgtattgaca gaaatcgcca
      841 accttgccca gcaaggcgtg aaagaaatca acctcttggg acaaaacgtc aacgcctatc
      901 gcggcgaaat ggacgacggc gaaatctgcg acttcgccac cctgctgcgc atcgtccacg
      961 aaatccccgg cattgaacgt atgcgcttca ccaccagcca cccgcgcgag tttaccgact
     1021 cgattatcga gtgctaccgc gacctgccca aattggtttc ccacctgcac ctgccgattc
     1081 aaagcggttc cgaccgcgta ttgagcgcga tgaaacgcgg ctacaccgct ttggaataca
     1141 aatccatcat ccgcaaactg cgtgccatcc gtcctgattt gtgcctgagc agcgatttca
     1201 tcgtcggctt ccccggcgag accgaacgcg agttcgagca aaccttgaaa ctggtgaaag
     1261 acatcgcctt cgacttgagc ttcgtgttta tttacagtcc gcgccccggc acgcctgccg
     1321 ccaacctgcc ggacgacacg ccgcacgaag aaaaagtgcg ccgcctcgaa gccttgaacg
     1381 aagtcatcga agccgaaacc gcgcgcatca accaaacgat gatcggcacg gttcaacgct
     1441 gcctggtcga aggcatctcc aaaaaagacc ccgaccagct ccaagcccgc accgccaaca
     1501 accgcgtcgt caactttacc ggcacgcccg acatgattaa ccaaatgatc gatttggaaa
     1561 tcaccgaggc ctacaccttc tccctgcgcg gcaaagttgt cgaagcctaa acccttacgc
     1621 cgaaaaaatg ccgtctgaag cgtttcagac ggcattttgc cttgtatcgg cagacgatgg
     1681 cgcggctagg cggcttaatt tgccgcatcc cgatccgaca accacgcgcg cacacgccgt
     1741 tccaccgctt cggcactcaa gcccaaatcg tctaaaagtt ttttcggatc gccgtgtccg
     1801 gttacggtat cggcaacgcc caaaagcaaa acgggtttgc agatgccgtg tttcgccaat
     1861 acttccagca ccgcgccgcc tgcgccgccc tgttcggcgt tttcttcaag ggtaacgatg
     1921 cggtcgtggc ttcgggcaag gcggacaatc aactcttcgt ctatcggttt gacgaagcgc
     1981 atatcggcga cggtggcgtt cagtttttcg gcaaccgcca atgcgggggc gaccatactg
     2041 ccgaaggcaa tgaaggcggt tttcccacct tcgcggcgga taatgccctt gccgatttcc
     2101 acggtttcca tgccgtctga aaccggcgcg cccgtacccg tgccgcgcgg atagcggacg
     2161 gcggcgggcg catccgcctg atagcaggtc gaaagcagca ggcggcattc gttttcatcg
     2221 ctcggcgcgg cgacaatcat attcggaatg cagcgcaaaa agctcaaatc gtacagaccg
     2281 gcatgggtcg ggccgtccgc gccgacgatg cccgcgcggt cgacggcaaa caaaacgggc
     2341 aggttttgca gggcgatgtc gtgcaccagt tggtcgtagg cgcgttgtaa aaaggtggaa
     2401 taaatcgcca cgacgggctt catgccttcg cacgccaaac cgccggcaaa ggtaacggcg
     2461 tgctgctcgg caatgccgac atcgaaataa cggtcgggga atcgttgttc aaactccacc
     2521 agtccgctgc cctcgcgcat cgcgggcgtg atggcgatca aacgctcgtc tgccgccgcc
     2581 cggtcgcaca gccatttgcc gaacacttgg gtataggtcg gtttggcggc gggcttgggt
     2641 tctttttcag acggcatttg cgccgcgctt tctttaggca ggttggcgac ggcgtggtat
     2701 ttgacgggat cgttttcggc gagtttgtag ccgttgccct ttttggtgat gacgtgcagc
     2761 agttgagggc ctttgcggct gcgcaagtct ttcaatacgt ccaccagatt ttcgacgttg
     2821 tgtccgtcca cggggccggt gtagcggaag ccgaagtttt caaacaaaga cagcgactgt
     2881 ttggcgtgtt cggcttcttc ggcaagggtt ttgattttgt gttcgacttt ttgggcgagc
     2941 tccatcgcgc cgggtatttt gtctaatacc ttgcccgttt gcgctttgac ggtactcaac
     3001 aggccgtgca tatcgcgcac gacgttgctg gcgaggtatt tcggcagcgc gccgacgttg
     3061 ggggaaatcg acatttcgtt gtcgttgagg acgaccagca aatccacatc catatcgcct
     3121 gcgcaattca aggcttcaaa cgcctgcccc gccgtcatcg cgccgtcgcc gatgatggcg
     3181 acgctgcggc ggtcgctgcc caagagtttg tctgccgccg ccatgcccaa cgccgcgccg
     3241 atggatgtgg aagaatgccc tacgccgaac gcgtcgtatt ccgactcgct gcgttttggg
     3301 aagccagcta aaccgccgta ttggcgcatg gtgtgcatct ggtttttcct gcctgtcagg
     3361 attttgtgcg gatagctttg gtgtccgaca tcccacacca gcttgtcttc gggcgtgtcg
     3421 tacacatagt gcagggcgat ggtcagttcg accgcgccca aattgctggc gaaatgcccg
     3481 ccggtctgcc cgacagattc cagcagaaag gtgcgcaact cgccggcaag gcgcggcagc
     3541 tgttttttgt ccagacggcg caaatcttgc gggctgtcaa tcaggtcgag tagggggctt
     3601 gggttcatgg tgtgtctttt ttatgtgtcg tccaggtgca acgaccaatt atatatcaag
     3661 agcgtgcggc tgacggctga ttttgccgta tgtcattcgt cctgccgctt ggcgcgcggg
     3721 tgggcttcgt catacaggcg ggcgatgtgg tcgaaatcga gtttggtgta aatctgcgtg
     3781 gtcgaaaggc tgctgtgccc gagcagctcc tgcaccgccc ggatgtcgcg cgaagcctgc
     3841 aacaggtgtc cggcgtagct gtggcgcatc atatgcggcg aaacgtgcct gccgtcgccg
     3901 ttttgcgccg cccattgcgc caaacgtttt tggatttggc gttggctcag gcgcgtgccg
     3961 ttcctgccgg taaacagggc tttgccgtcc gatgccgtct gacgcagcgg cagatagttt
     4021 ttcagggctt ccacgctttt gccgaccagc ggcacctgcc gctgcttgcg ccctttgccg
     4081 gtaacgtgta cccacgcctc gtccaaatag acatcatctg cattcaagcc gtgtatctcg
     4141 ctcacgcgca aaccgctgcc gtacatcagc tcgaacaggg cgtgatcgcg caccgccagc
     4201 gggtcgccgc cgtccacggg caaatccagc atccggttca gccattcctg cgacagggct
     4261 ttgggtacgc gctcgggctg cttcggcggt ttgatgtcgg cggtcgggtc ggtatgcagc
     4321 agaccgcgtt ccaccagcca aacacaatac tgccgccaag acgacagctt gcgcgccagc
     4381 gtccgcgcat tcaaaccgcg ctgcgacagc cgccgcaacg ccgccgtaaa atcgcgccgc
     4441 gacaagtcct gcggcacgcc gccttcatct tcagacggca tttgtgccaa cagcgcaaac
     4501 agttcttcca aatcgcgccg gtatgccgcc accgtgtgct ccgacttgcc ctcgcgcacg
     4561 atgttttcca aataagcgtc aaaatgcgcc gcaaacccgt ccaaaaccat aaccgcccca
     4621 cacaaatatc aaaaaaacag tgaaaacatt atcataaacg acacaccaaa accccgaacc
     4681 gcccaaaatc cgacccgtcc gcccgccgtt atcgttaaca taaaatcaat gagccgttta
     4741 tggttttttg ccgcaaaaaa cattataatc cgccttattt acctattgcc caaggagaca
     4801 caaatggcac tcgtatccat gcgccaactg cttgatcatg ctgccgaaaa cagctacggc
     4861 ctgcccgcgt tcaacgtcaa caacctcgaa caaatgcgcg ccattatgga agccgccgac
     4921 caagtcaacg cgcccgtcat cgtacaggcg agtgcaggtg cgcgcaaata tgcgggcgcg
     4981 ccgtttttgc gccacctgat tctggcggct gtcgaagaat ttccgcacat ccccgtcgtg
     5041 atgcaccaag accacggcgc atcgcccgac gtgtgccaac gctccatcca actgggcttt
     5101 tcctccgtga tgatggacgg ctcgctgatg gaagacggca aaaccccttc ttcttatgaa
     5161 tacaacgtca acgccacccg taccgtggtt aatttctccc acgcttgcgg cgtatccgtc
     5221 gaaggcgaaa tcggcgtgtt gggcaacctc gaaaccggcg aagccggcga agaagacggt
     5281 gtaggcgcag tgggcaaact ttcccacgac caaatgctca ccagcgtcga agatgccgtg
     5341 cgtttcgtta aagataccgg cgttgacgca ttggcgattg ccgtcggcac cagccacggc
     5401 gcgtacaaat tcacccgtcc gcccacaggc gacgtgttgc gtatcgaccg catcaaagaa
     5461 atccaccaag ccctgcccaa tacacacatc gtgatgcacg gctccagctc cgttccgcaa
     5521 gaatggctga aagtcatcaa cgaatacggc ggcaatatcg gcgaaaccta cggcgtgccg
     5581 gttgaagaaa tcgtcgaagg catcaaacac ggcgtgcgca aagtcaacat cgacaccgac
     5641 ttgcgccttg cttccaccgg cgcggtacgc cgctaccttg ccgaaaaccc gtccgacttc
     5701 gatccgcgca aatacctgag caaaaccatt gaagcgatga agcaaatctg cctcgaccgc
     5761 tatcttgcgt tcggttgcga aggtcaggca ggcaaaatca aaccggtttc cttggaaaaa
     5821 atggcaagcc gttatgccaa gggcgaattg aaccaaatcg tcaaataaca ggttgcttgt
     5881 aaacaaaatg ccgtctgaac cgttgttcgg acggcatttg atttttgctt ctttgacctg
     5941 cctcattgat gcagtatgca aaaaaagata ccataaccaa aatgtttata tattatctat
     6001 tctgcgtatg actaggagca aacctgtgaa ccgaactgcc ttctgctgcc tttctttgac
     6061 cgccgccctg attctgaccg cctgcagcag cggaggcggc ggtgtcgccg ccgacatcgg
     6121 cgcggggctt gccgatgcac taaccacacc gctcgaccat aaagacaaaa gtttgcagtc
     6181 tttgacgctg gatcagtccg tcaggaaaaa cgagaaactg aagctggcgg cacaaggtgc
     6241 ggaaaaaact tatggaaacg gcgacagcct caatacgggc aaattgaaga acgacaaggt
     6301 cagccgcttc gactttatcc gtcaaatcga agtggacgga caaaccatca cgctggcaag
     6361 cggcgaattt caaatataca aacagaacca ctccgccgtc gttgccctac agattgaaaa
     6421 aatcaacaac cccgacaaaa tcgacagcct gataaaccaa cgctccttcc ttgtcagcgg
     6481 tttgggcgga gaacataccg ccttcaacca actgcctgac ggcaaagccg agtatcacgg
     6541 caaagcattc agctccgacg acccgaacgg caggctgcac tactccattg attttaccaa
     6601 aaaacagggt tacggcagaa tcgaacacct gaaaacgccc gagcagaatg tcgagcttgc
     6661 ctccgccgaa ctcaaagcag atgaaaaatc acacgccgtc attttgggcg acacgcgcta
     6721 cggcggcgaa gaaaaaggca cttaccacct cgcccttttc ggcgaccgcg cccaagaaat
     6781 cgccggctcg gcaaccgtga agataaggga aaaggttcac gaaatcggca tcgccggcaa
     6841 acagtagcat ttgaaaaatg ccgtccgaac acgatagttt accgttcgga cggcattttt
     6901 gcgccatact gccctaggac acgacggcac ggcaggcaag ccggtttcac tatataatgc
     6961 cgtctgaacc aacgagaacc acaccatgca agccgatttc aaccgccccg tcctcgccgt
     7021 cgataccgga acttcctatt tgtcgctcgc gctgcgcgcc gacggcgaaa ccctcctgtt
     7081 ccatcaggag gccggcagcc gccagtccga actgatcctg ccggaaatcc gcaccctatt
     7141 ccgaaatgcg ggcattaccg ccgccgattt gggggcgatt gtttatgcga aaggtcccgg
     7201 cgcgtttacc ggactgcgta tcggcatcgg cgtggcgcaa ggtttggcaa cgccattcaa
     7261 tacgccttta atcggcatcc ccacgctcga tgccgccgcc tcgctgccgc cgccgcaaag
     7321 ctgcatactc gccgctgcgg acgcgcgtat gggcgaagtg ttttatgcgt ggttcgacac
     7381 gctgaaccgc cgccgtttga gcgattatca ggtcgggcgg gcggcagaca tccggctgcc
     7441 ggagggatgt gccttttcag acggcatagg cagcgcgttc gcgttggaag aaacgccgcc
     7501 gttttcaggc aaacccgata tgccgaccgc cgccgacttc ctcaatctgg cggcggaagg
     7561 cggttttccc gccgtcgctg ctgcacactc cgaactgctc tacgtccgca acaaaatcgc
     7621 cctaaccgcc aaagaacagg cggaacggaa agcccgaaca tgaacatccg ccgcgcctca
     7681 ccgtccgact gcgccgcact cgccgcgctc gatgccgtct gcaacccgtc cgcgtggacg
     7741 caacgccaat ttgagtccgc attggtttcg ccgtccgaac aggttttcct cgcggaaaaa
     7801 gacggcggga ttgccgcctt tatcgtttgg cagaacctgc ccgacgaatc cgaactgcac
     7861 ctgattgcca ccgcgcccga atgccgccgc cgtggcgttg cgtccgccct gctcgaatat
     7921 tggttcgcac atctgcccga agacacgcaa cgcctgctgc tcgaagtccg tgcaggcaac
     7981 accgccgcac aggcactgta cgcggcgcac ggctttaccg atgcgggcag gcggaaaaac
     8041 tattaccgta cagccgacgg taaaaccgaa gatgctgtct taatggagaa aatatgttaa
     8101 gcgcgcgcta cctccacctg cacgaagctt tgggtttagg cccgatgtgg ctgaaacagg
     8161 ccgccgccgt cctgccgccc aaaaacacac ccgcaacccc ggcacaggca cgtcctcaaa
     8221 ccgtccgcgc cgccccgatc cgcccttccc aaccccataa cggtcaggcg cggctcgaaa
     8281 cgatgaaagc gttggaaacc gccgccgtac atacgcgcaa acccgcgcct gaaaccgaaa
     8341 cgcccccgcc cggcctttca gacggcatcg cccccgttcc cgccgcttcg ggcataacca
     8401 aacttgccgt cgtcagcctt tgcccaccga ttgaggatgc ggtttacggg caactgttct
     8461 acggcaaagc gggtgttctg cttgacaaca tactcaaagc cgtaggactg gatgccgcct
     8521 atgtccacaa aacctgttgg gtgaaaaccg ccgccgtcgg caacccgatg ccgtctgaag
     8581 cggcaatcga aaccgcgacg gtttccgtca tgcaggaact cgacggctgc cgcgcccccg
     8641 ccgtcctatt cctcggacag gcttttgtcc agcctgaacg gcaaacgatg attgaaactt
     8701 tgtgcggcag ccgtcccttc ttcatcatcg accatcccgc ccggctgttg cgccaacccg
     8761 aacttaaagc ccgcgcctgg caggtgttga aacagttgaa acgcgccttg cggcaaggcg
     8821 gcggcagttg aagcgcaccg cacggggcgg tagaatcgca actgcgtccc aatatctgac
     8881 agaaagcaca aaatgaccga tttccgccaa gatttcctca aattctccct cgcccaaaat
     8941 gttttgaaat tcggcgaatt taccaccaaa gccggacggc ggtcgcccta tttcttcaat
     9001 gccggcctct ttaacgacgg cttgtccacg ctgcaactgg caaaatttta cgcacaatcc
     9061 atcattgaaa gcggcatccg attcgatatg ctgttcggcc ccgcctacaa aggcattatt
     9121 ttggcggcgg caaccgcgat gatgctggcg gaaaaaggcg tgaacgtccc gtttgcctac
     9181 aaccgcaaag aagccaaaga ccacggcgaa ggcggcgtgt tggtcggtgc gccgcttaaa
     9241 ggccgcgtgc tgattatcga cgacgtgatt tccgccggca catccgtacg cgaatcgatc
     9301 aagctgattg aagcggaggg cgcaaccccc gccggtgtcg ccatcgcgct cgatcgcatg
     9361 gaaaaaggca cgggtgagtt gagcgcggtt caggaagtgg aaaaacaata cggtctgccc
     9421 gtcgccccca tcgccagcct gaacgatttg tttattctgt tgcaaaacaa ccccgaattc
     9481 ggacagttcc tcgaacccgt ccgagcctac cgtcggcagt acggcgtaga ataaaaacaa
     9541 agcatatgcc gtccgaaccg ccttacgcct cagacggcat caaacctgac acacacgagg
     9601 aaataccatg cccgcctgtt tctgccccca ctgcaaaacc cgtctctggg tcaaagaaaa
     9661 ccaactcaat gtcgcccaag gcttcgtcgt ctgccaaaaa tgcgaaggac tgtttaaagc
     9721 caaagaccat ctggcaagca cgaaagaacc catattcaac gatttgcccg aggctgtttc
     9781 ggatgtcaaa ctcgttcacc gtatcggcac gcgcgccatc ggcaagaaac agatttcccg
     9841 tgacgaaatc gccggcatcc tcaacggcgg cacaacccag cccgatattc cgcccgcaac
     9901 cgccgccacc cctgctgccg caccgcaggt taccgtaccg cccgccgcgc ccgcccgtca
     9961 ggatgggttc aactggacga ttgcaaccct gtttgccctt atcgtcctca ttatgcagct
    10021 ttcctacctc gtcatcctat gatcgtgccc gacctctttg tcgcccactt ccgcgaagcc
    10081 gccccctaca tccgccaaat gcgcggcaaa acgctggtcg ccggcataga cgaccgcctg
    10141 ctcgaaggtg ataccttaaa caagctcgcc gccgacatcg ggcttttgtc gcaactgggc
    10201 atcaggctcg tcctcatcca cggcgcgcgc cacttcctcg accgccacgc cgccgcgcaa
    10261 ggccgcacgc cgcattattg ccggggcttg cgcgttaccg acgaaacctc gctcgaacag
    10321 gcgcagcagt ttgccggcac cgtccgcagc cgttttgaag ccgcattgtg cggcagcgtt
    10381 tccgggttcg cgcgcgcgcc ttccgtcccg ctcgtatcgg gcaacttcct gaccgcccgt
    10441 ccgataggtg tgattgacgg aaccgatatg gaatacgcgg gcgttatccg caaaaccgac
    10501 accgccgccc tccgtttcca actcgacgcg ggcaatatcg tctggctgcc gccgctcgga
    10561 cattcctaca gcggcaagac cttccatctc gatatgcttc aaaccgccgc ctccgtcgcc
    10621 gtctcgcttc aggccgaaaa actcgtttac ctgacccttt cagacggcat ttcccgcccc
    10681 gacggcacgc tcgccgtaac cctctcggca caggaagcgc aatcgctggc ggaacacgcc
    10741 ggcggcgaaa cgcgacggct gatttcgtcc gccgttgccg cgctcgaagg cggcgtgcat
    10801 cgcgtccaaa tcctcaacgg tgccgccgac ggcagcctgc tgcaagaact cttcactcgc
    10861 aacggcatcg gcacgtccat tgccaaagaa gccttcgtct ccatccggca ggcgcacagc
    10921 ggcgacatcc cgcacattgc cgccctcatc cgcccgctgg aagaacaagg catcctgctg
    10981 caccgcagcc gcgaatacct cgaaaaccac atttccgaat tttccatcct cgaacacgac
    11041 ggcaacctgt acggttgcgc caccctaaaa acctttaccg aagccgattg cggcgaaatc
    11101 gcctgccttg ccgtctcgcc gcaggcacag gacggcggct acggcgaacg cctgcttgcc
    11161 cacattatcg ataaggcgcg cggcataggc ataagcaggc tgttcgcact gtccacaaat
    11221 accggcgaat ggtttgccga acgcggcttt cagacggcat cggaagacga gttgcccgaa
    11281 acgcggcgca aagactaccg cagcaacgga cggaactcgc atattctggt acgtcgcctg
    11341 caccgctgac cgcaacggaa agccgccgca gaaatgccgt ctgaaccccg tttcagacgg
    11401 catttccccg attataaaga cctgcccaac cctcaaggac cccgatgaaa tcctaccccg
    11461 acccctaccg ccattttgaa gacctcgatt ccgccgaaac gcaaaacttc gctgctgaag
    11521 cgaatgccga aacgcgcgcg cgttttttaa acaacgacaa ggcgcgcgca ttgtctgacg
    11581 gtattttggc gcagttgcag gacacgcggc agattccgtt ttgtcaggaa caccgcgcgc
    11641 ggatgtacca tttccatcag gacgcggaat atccgaaggg cgtgtaccgc gtgtgtaccg
    11701 cggcgacata ccgttccggc tatcctgagt ggaaaatcct gttttcagtg gcggatttcg
    11761 acgaattgct cggcgacgat gtatatttgg gcggcgtgtc gcacttggtg gaacagccca
    11821 accgtgcgtt gttaacactg agcaaatcgg gcggcgatac ggcgtacacg ctggaagtgg
    11881 atctggaagc aggggagttg gtagaaggcg gttttcactt tccggcaggc aaaaaccatg
    11941 tgtcgtggcg cgatgaaaac agcgtgtggg tgtgtccggc ttggggcgaa cgccagttga
    12001 ccgaatcggg ctatccgcgc gaggtgtggc tggtggaacg cggcaagagt ttcgaggaaa
    12061 gcctgcctgt gtatcaaatc gataaaggtg caatgatggt gaacgcgtgg cgttatctcg
    12121 atccgcaagg ttcgccgatt gatttgattg aagcgtcgga cggtttttac accaaaacct
    12181 atttgcaggt gtcgtcggaa ggcgaggcga aaccgttaaa cctgcccaac gattgcgacg
    12241 tggccggcta tctagccgga catcttttgc tgacattgcg taaagactgg caccgcgcga
    12301 accaaagcta tccgagcggc gcattggtgg cagtaaaatt aaaccgaggc gaactcgggg
    12361 cggcgcagct tttgtttgcg cccgatgaaa cgcaggcatt ggaaagcgtg gaaacgacca
    12421 agcgttttgt ggtagcgagc ctgctggaaa acgtacaagg ccgtctgaaa gcgtggcggt
    12481 ttgccgacgg caaatggcag gaagtcgaac taccgcgcct gccttcgggc gcgttggaaa
    12541 tgaccgacca accgtggggt ggcgacgtgg tttaccttgc cgccggcgat ttcaccacgc
    12601 cgctggcgct gtttgcattg gatttgaacg tgatggaact gaccgtcatg cgccgtcagc
    12661 cgcagcagtt cgattcagac ggcatcaacg tgcggcagtt ttgggcggtt tcgtccgacg
    12721 gcgagcgcat tccttatttc catgtcggca aaaacgccgc gcccgacacg ccgactttgg
    12781 tctatgctta cggcggtttc ggcgtgcccg agctgccgca ttatctgggc agcgtcggca
    12841 aatactggct ggaagagggc aatgccttcg tgttggcgaa catacgcggc ggcggcgagt
    12901 tcggcccgcg ctggcatcag gcggcgcagg gcatcagcaa acacaaaagc gtcgatgatt
    12961 tgcttgccgt cgtgcgcgat ttgtccgaac gcggcatcag ttcgcccgaa cacatcggtt
    13021 tgcagggcgg cagcaacggc ggactgatta ccgccgccgc cttcgtgcgc gaaccgcaaa
    13081 gcatcggcgc gctggtgtgc gaagtgccgc tgaccgatat gatccgttat ccgctgctct
    13141 ccgccggttc aagctggaca gacgaatacg gcaatccgca aaaatacgaa gtctgcaaac
    13201 gccggctggg cgaattgtcg ccgtatcaca atctttcaga cggcatcgat tatccgcctg
    13261 cgctcatcac caccagcctc agcgacgacc gcgtccatcc cgcccacgcg ctcaagttct
    13321 acgccaaact gcgtgaaacc tccgcgcaat cttggctcta ctctccggac ggcggcggcc
    13381 ataccggcaa cggcacccaa cgcgaatccg ccgacgaact cgcctgcgtg ttgctgtttt
    13441 tgaaagagtt tttgggctaa gggcggggag cggcactgcc gccgcgaatg aaaaaaggtc
    13501 gtctgaaact gctttttcag acgacctttt ttaatggttg tttcaaatca aaatatctat
    13561 gccgccggcc ccatcagcac ttcttcgcat ccgaaggcaa aaatccgtaa tgccgcctga
    13621 acgcttcatt gagccgtccc gcgtggcggt agccgcaaaa gtgcatggcg gcttggacgg
    13681 tgctgccgga ttcgatgaag gcgagcgcgt gttccagccg caggcggcgc aggcatccgg
    13741 cgacggtttc gccggtttgc gctttgaaat agcgtttcag gtagcattcg ttcagtccga
    13801 cgcggcgggc gatttcggcg atggtcagcg gacgggcgaa ttcgtgttgc aggatgtcgg
    13861 cggcttcgtc tatgcgccga cggcggtaac cgttgtcgtg gcggcggaag gtgaagcgca
    13921 ataatcgggc ggagagttcc agcgcggcgg cttcgtcggc aagcaggccg aagccgtccg
    13981 attcgaacgg gcgttgcagc agtgggcagg ccgccgccgt cagtgccgct gcgttttgcg
    14041 ccagccgttg cagggcgaat cggcctatcg tttgcggcga aaacaggcgt tcgtccagca
    14101 agccttcgtc gtgccagcgg cgcagttttt ccagcgaaaa atccaaatgc agcgcgcaca
    14161 tgccgctgtt gtcgggcagc agggtttcgg atacgtccgc caactcgccg cgtaccagcc
    14221 agatttcgcc ggcagacggg cggtattccc tgccgcccat ttgtaaccgg ttctgccccg
    14281 acaccatgac gaacaaggcg cagttgtggc tgaaattgtg gatttcggtg ggaaacgcgc
    14341 ccgttccgcc gccgcgcatc cgcgacaagg tgatgcccga gtcgaagcgg ttgatgcaca
    14401 tttccagatg caaaccgggc tgttttgcct gcgcaatgag cgcgctgtcg gaacagccgt
    14461 ccaacgccca gccggattta tcggagcgga cataggtttg gtactggcgg tagatggcgg
    14521 cggtgttcat gattggatag gaacgagttg tctaacaaat gaattaaata ggaattatta
    14581 ccaataatca agcgcaggga ttggttgaaa cggaaaaggt cgtctgaaag ggtgtttcag
    14641 acgacctttt ccgtatcggg aatttgtttt gccgtatcgg gaattttgcg ttttgcggcg
    14701 tggtttctgc aggttgtttg cttaataata aacattctta ttcgtatgca aaggaaccgc
    14761 acaccgtgaa accgcgtttt tattgggcag cctgcgccgt cctgctgacc gcctgttcgc
    14821 ccgaacctgc cgccgaaaaa actgtatccg ccgcatccgc atctgccgcc acactgaccg
    14881 tgccgaccgc gcggggcgat gccgttgtgc cgaagaatcc cgaacgcgtc gccgtgtacg
    14941 actgggcggc gttggatacg ctgaccgaat tgggcgtgaa tgtgggcgca accaccgcgc
    15001 cggtgcgcgt ggattatttg cagcctgcat ttgacaaggc ggcaacggtg gggacgctgt
    15061 tcgagcccga ttacgaagcc ctgcaccgct acaatcctca gcttgtcatt accggcgggc
    15121 cgggcgcgga agcatatgaa cagttggcga aaaacgcgac caccatagat ctgacggtgg
    15181 acaacggcaa tatccgcacc agcggcgaaa agcagatgga gaccttggcg cggattttcg
    15241 gcaaggaagc gcgcgcggcg gaattgaagg cgcagattga cgcgctgttc gcccaaacgc
    15301 gcgaagccgc caaaggcaaa ggacgcgggc tggtgctgtc ggttacgggt aacaaggtgt
    15361 ccgccttcgg cacgcagtcg cggttggcaa gttggataca cggcgacatc ggcctaccgc
    15421 ctgtagacga atctttacgc aacgaggggc acgggcagcc tgtttccttc gaatacatca
    15481 aagagaaaaa ccccgattgg attttcatca tcgaccgtac cgccgccatc gggcaggaag
    15541 ggccggcggc tgtcgaagta ttggataacg cgctggtacg cggcacgaac gcttggaagc
    15601 gcaagcaaat catcgtcatg cctgccgcga actacattgt cgcgggcggc tcgcggcagt
    15661 tgattcaggc ggcggagcag ttgaaggagg cgtttgaaaa ggcagaaccc gttgcggcgg
    15721 ggaaagagta gggagtcgtc tgaaaacgga gtttccgaag gaagcggggg gtttctgcga
    15781 agctaaagtg cggtttcaac gaattgaaaa gcagcctgta tgttgaaaat accgctcaag
    15841 caaacctacg gtttgccgcc ctctccctag ccctctccca cagggagagg ggattggggt
    15901 gcaggctgcc tttaaggttt aggcaaattt ttaacttcgt tgaagctgcg atttcagaag
    15961 ctccgttttc agacgacctt ttggaacacc acaggcacac gcatcgaacg gctgaatcaa
    16021 agattcagac cgatggcagt ccgcacc
//
LOCUS       DAXHTR010000034        15797 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.034, whole genome shotgun sequence.
ACCESSION   DAXHTR010000034 DAXHTR010000000
VERSION     DAXHTR010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.034"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            372..5024
                     /gene="iga"
                     /locus_tag="ACFNML_000748"
     CDS             372..5024
                     /gene="iga"
                     /locus_tag="ACFNML_000748"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HFC6392161.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNRRTLTVIDPQYAVSVKHV
                     KGDEISYYGHHNGHLDVSNDENEYRSVAQNDYEPNKNWHHGNQGRLEDYNMARLNKFV
                     TEVAPIAPTSAGGGVETYKDKNRFSEFVRVGAGTQFEYNSRYNMTELSRAYRYAIAGT
                     PYQDVNVTSNLNQEGLIGFGDNSKHHSPEKLKEVLSQNALTNYAVLGDSGSPLFAYDK
                     QEKRWVFLGAYDYWAGYQKNSWQEWNIYKKEFADEIKQRDNAGTIKGYGEHHWKTTGT
                     NSHIGSTAVRLAGNERGANNGQNVTFENNGTLVLDQNINQGAGGLFFKGDYTVKGINN
                     DITWLGAGIDVADGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQGQLKVGDGTVIL
                     NQKADSNQKVQAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGRLDANGNDLTFEH
                     IRNVDEGARIVNHNTGHTSTITLTGKSLITNPNSLSVHSIQNDYDEDDYSYYYRPRRP
                     IPQGKDLYYKNYRYYALKSGGSVNAPMPENGQTENNDWILMGSTQEEAKKNAMNHKNN
                     QHISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGKISVTQGNVLLSG
                     RPTPHARDFVNKSSARKDAHFSKNNEVVFEDDWINRTFKATEIAVNQSASFSSGRNVS
                     DITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTGNLSDKALNSFGATQINGNV
                     NLNQNAALVLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNLSLADSHIHLNNA
                     SDAQSANQYHTLKINHLSGNGHFHYLTHLAKNLGDKVLVKESASGHYQLHVQDKTGEP
                     NQEGLNLFDASSVQDRSRLSVSLANNHVDLGALRYTIKTENGITRLYNPYAENRRRVK
                     PAPSPATNTASQAQTDSAQIAKPQNIVVAPPSPQANQAEEAKRQQAKAEQVKRQQAEA
                     EKVAHQKAEEAKRQQDALARQQAEQERQRLEAERQAAEIAKQKAEAEEAKRRAAEIAE
                     QKAAAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAEIAEQKAEAEREAAELA
                     KQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIARNSNPDASDYEEIPLDA
                     LEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQAAAQPQAQAVAQADAV
                     STNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNSVWMSNIGYGRDYASAQ
                     YRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQASGKNTFVQANLYGKYYL
                     NDAWYVAGDIGAGSLRSRLQTQQKANFNRTSIQTGLTLGNTLKINQFEIVPSAGIRYS
                     RLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVKPLLSAAYFANYGKGGV
                     NVGGNSFAYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQLEKQKSGQIKIQIRF"
     gene            complement(5203..6405)
                     /gene="trpB"
                     /locus_tag="ACFNML_000749"
     CDS             complement(5203..6405)
                     /gene="trpB"
                     /locus_tag="ACFNML_000749"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFC6392162.1"
                     /translation="MKNYHAPDEKGFFGEHGGLFVSETLIPALQELADAYKAAKNDPS
                     FWEEFHRDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVDSGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     EQMLEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGSPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(6488..7177)
                     /locus_tag="ACFNML_000750"
     CDS             complement(6488..7177)
                     /locus_tag="ACFNML_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HFC6392163.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDSVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAAREHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAVYRAF
                     FEMAGLCEKQL"
     gene            7374..8153
                     /gene="rsmA"
                     /locus_tag="ACFNML_000751"
     CDS             7374..8153
                     /gene="rsmA"
                     /locus_tag="ACFNML_000751"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFC6392164.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIAGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVDRMVAAPKSNDYGRLGVMLQYFFDM
                     EMLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELAGDDDLQAVGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8326..9054
                     /locus_tag="ACFNML_000752"
     CDS             8326..9054
                     /locus_tag="ACFNML_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6392165.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            9127..9486
                     /locus_tag="ACFNML_000753"
     CDS             9127..9486
                     /locus_tag="ACFNML_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392166.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            9491..9937
                     /locus_tag="ACFNML_000754"
     CDS             9491..9937
                     /locus_tag="ACFNML_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFC6392167.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLKITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAATLAGSSMVTPYALF
                     LQWDTGGKTVRHCIMPDMTDKESYRKLKVWVLWRQPKKTAETDTSD"
     gene            9968..11242
                     /gene="folC"
                     /locus_tag="ACFNML_000755"
     CDS             9968..11242
                     /gene="folC"
                     /locus_tag="ACFNML_000755"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFC6392168.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERTSEVKKRMKLEPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARSEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DLDHQAFLGDTVEQVGFEKAGVFRSGKPAICGQNPAPESLLSHAEAIGAKLLMVQRDF
                     EFHAMENIQWNYRFRPQHSDDPARNRNALPFPALRGTYQLSNAACTLTVLECLNAKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLETVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            11255..12253
                     /gene="ftsN"
                     /locus_tag="ACFNML_000756"
     CDS             11255..12253
                     /gene="ftsN"
                     /locus_tag="ACFNML_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFC6392169.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESRKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAVGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            12246..12743
                     /locus_tag="ACFNML_000757"
     CDS             12246..12743
                     /locus_tag="ACFNML_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HFC6392170.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFVIACLIQKMLRSLLTGAVSAVGLGFANR
                     ILGGVFGALKGVLIVTLLVMLASKTDLPDTEEWQQSYTIPFFVSLSEAVLNHSGGTAE
                     TPEDD"
     gene            12951..14495
                     /gene="purF"
                     /locus_tag="ACFNML_000758"
     CDS             12951..14495
                     /gene="purF"
                     /locus_tag="ACFNML_000758"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFC6392171.1"
                     /translation="MCGVLGLVSHEPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLVGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHVNTSSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMLAFRDPYGIRPLVLGSQTDNEGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTMIARQCSDRAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVHLDKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQNLSDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            14595..15086
                     /gene="greB"
                     /locus_tag="ACFNML_000759"
     CDS             14595..15086
                     /gene="greB"
                     /locus_tag="ACFNML_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFC6392172.1"
                     /translation="MSTETKNYITPAGWQALKDELYQLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            15145..15771
                     /locus_tag="ACFNML_000760"
     CDS             15145..15771
                     /locus_tag="ACFNML_000760"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HFC6392173.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         4391 a         4300 c         3862 g         3244 t
ORIGIN      
        1 acgaaccccc atctcgtcat taccacgaac ccccatctcg tcattaccac gaacccccat
       61 ctcgtcatta ccacgaaagc gggaatctag gacgcagggt taagaaaacc tacatcccgt
      121 cattcccgca caagcgggaa tccaaaaaca aaaagtcgca gaaatttaac ggaagaaccg
      181 aaccccgaaa atcggcttcc cgccttcgct tgaataacag aacaaaggaa gcggaaataa
      241 aaacatcggg attctttttt taaattgaca ataaaacacc aaaattaatg atttacacaa
      301 caaaaaatag tcaacaccgc caaaccggcg taaaatacaa gacattatca cccttaaaac
      361 ggtaaaacct tatgaaaacc aaacgtttta aaattaacgc catatcctta tccatctttc
      421 ttgcctatgc ccttacgcca tactcagaag cggcattggt cagagacgat gtcgattatc
      481 aaatattccg tgactttgca gaaaacaaag gcaaattttt tgtcggcgca accgatttat
      541 cagtgaaaaa caaacaaggt caaaacatcg gcaacgcatt atccaacgta ccgatgattg
      601 attttagcgt tgcagatgtc aacagacgta cattaaccgt catcgatccc cagtatgccg
      661 tcagcgtcaa acatgtaaaa ggagacgaaa tctcttatta cgggcatcac aatgggcact
      721 tagatgttag caatgatgaa aacgaatatc gttcagttgc acaaaatgac tacgaaccaa
      781 ataaaaattg gcatcacggt aatcaaggtc gtcttgaaga ctacaacatg gcacgcctta
      841 ataaattcgt aacagaagtc gcacctattg caccaaccag tgcaggcggc ggcgttgaaa
      901 cctataaaga taaaaaccgt ttttctgagt ttgtgcgagt tggagcaggt acgcaatttg
      961 aatataacag ccgctacaat atgacagagc tttcacgagc ttatcgttat gccattgcag
     1021 gtacgcctta tcaagacgtg aatgtcacat cgaatctaaa tcaagaaggc ttgattggct
     1081 tcggtgataa ttcaaaacat cattccccag aaaaactcaa agaagtttta tctcaaaacg
     1141 cattgactaa ctatgctgtg ttaggcgata gcggttcacc attatttgct tatgataaac
     1201 aagaaaaacg ctgggtcttt ttaggtgctt acgattattg ggcaggctat caaaaaaact
     1261 cttggcaaga atggaatatc tataaaaaag aatttgcaga tgaaatcaaa caacgcgata
     1321 acgccggcac catcaaaggt tatggcgaac atcattggaa aaccacgggc acaaacagcc
     1381 atatcggttc gacagcggta aggcttgccg gcaatgaaag gggtgcaaac aacggacaga
     1441 atgttacctt tgaaaacaac ggcactttgg tattggatca aaacatcaac caaggcgcgg
     1501 gcggtctgtt tttcaaaggc gattacacag tcaaaggtat aaataatgac atcacttggc
     1561 taggtgcggg gattgatgtt gccgacggca aaaaagtcgt ttggcaagtc aaaaatccga
     1621 atggcgacag attggcgaaa atcggcaaag gcacattaga aataaacggc acaggcgtta
     1681 accaagggca attaaaagtc ggcgacggta cggttattct gaatcaaaaa gccgattcaa
     1741 accaaaaagt ccaggctttc tcccaagtcg gcattgtcag cggacgcggt acattggtat
     1801 taaatagttc aaatcagatt aatcccgata acctatattt cggtttccgt ggcggtcgtt
     1861 tggatgccaa tggcaatgac ttgacttttg aacacatccg caacgtggat gaaggcgcgc
     1921 gcattgtcaa ccacaacaca ggccacacct ccacaatcac gctaacgggt aaatctttaa
     1981 ttaccaatcc gaacagcctt tcggtacata gtatacaaaa tgactatgat gaagatgatt
     2041 atagttatta ttaccgacca aggcgaccca tcccacaagg caaagatctt tattacaaaa
     2101 actatcgtta ttacgcccta aaatccggcg gcagcgtgaa cgcaccgatg cccgaaaacg
     2161 gacaaacgga aaataacgac tggattttaa tgggcagcac acaagaagaa gccaagaaaa
     2221 acgcgatgaa ccacaaaaac aatcagcata ttagcggttt tagcggtttc tttggcgaag
     2281 aaaacggaaa aggacataac ggtgcattaa accttaattt caacggcaaa agcgcgcaaa
     2341 accgtttctt gttaacaggt ggcactaatt taaacggaaa aataagcgta actcaaggca
     2401 atgtcttgtt atcaggtcgt ccaacaccac acgcaagaga ttttgtgaac aaatcttcag
     2461 cccgaaaaga cgcacatttc tccaaaaaca atgaagtcgt atttgaagac gactggataa
     2521 accgcacatt caaagccaca gaaattgcgg ttaatcaatc cgcatcattc tcttccggca
     2581 gaaatgtatc cgacatcacc gccaacatca ccgcaacaga caacgccaaa gtaaatttgg
     2641 gttacaaaaa cggcgatgag gtttgcgtgc gctcggacta taccggttat gttacctgca
     2701 acacaggcaa cttatccgat aaggctttaa acagctttgg agcgacacag attaacggca
     2761 atgtgaatct gaaccagaat gcggcattgg tcttgggcaa ggctgcatta tgggggcaaa
     2821 ttcaagggca aggaaacagc cgtgtcagcc taaaccaaca tagcaaatgg catttgactg
     2881 gcgacagcca agtacacaat ctgtcattgg cggatagcca tattcatttg aacaatgcct
     2941 ccgatgcgca aagtgcaaat caataccaca cgctcaaaat caatcattta tccggtaacg
     3001 ggcattttca ttatctgacg cacctggcga aaaatcttgg ggataaagtg cttgtgaagg
     3061 aatccgcatc cggtcattat cagctccatg ttcaagataa aacaggcgaa cccaatcagg
     3121 aagggctgaa tctctttgat gcatcatccg tacaagaccg ctcccgcctt tctgtttcct
     3181 tggcgaataa ccatgtagat ttaggggcat tgcgttatac aatcaaaaca gaaaacggta
     3241 ttacccgatt gtacaatcct tatgccgaga accgccgccg agtcaaaccg gccccgtctc
     3301 ctgccacaaa cacggcttct caagcacaaa cggacagtgc acaaattgcc aagcctcaaa
     3361 atatcgtcgt cgcaccgcct agcccgcagg caaatcaagc cgaagaagca aaacgccaac
     3421 aagcaaaagc ggagcaagtg aagcgtcagc aagcagaggc agaaaaagtc gcacatcaaa
     3481 aggctgaaga agcgaaacgt cagcaagatg cccttgcccg ccaacaagca gagcaagaac
     3541 gccaacgatt ggaagccgaa cgccaagctg ccgaaattgc caaacaaaaa gcagaagccg
     3601 aagaagcgaa acgccgagcc gctgaaattg ccgagcaaaa agcagctgcc gaagaagcaa
     3661 aacgccaagc tgccgaactt gcacgccaac aagaagaagc acgaaaagct gcagagttgg
     3721 ctgctaagca aaaagcggaa actgaacgca aagcagcaga aattgccgag caaaaagcgg
     3781 aagccgaacg tgaagccgcc gaacttgcca agcaaaaggc ggaagaagaa ggtcgccaag
     3841 cagcacaatc tcaacccaaa cgtcgtaacc gcagggctat accaccggaa ctgtcatctg
     3901 atgcaacaac ccgagctttg cctaggatag cgagaaattc aaatcctgat gccagcgatt
     3961 atgaagaaat ccccttggat gcgttggaag atgaagatgt atccgaatcg gttgacacat
     4021 cagacaaaca gcctcaagac aatacggaac ttcatgaaaa agttgagaca gtgagtttgc
     4081 aaccaagagc cgctcagccg cgagcccaag ccgccgcgca gccgcaagcc caagccgtcg
     4141 cgcaagccga tgcagtcagc accaatacca actcggcttt atctgacgca atggcaagca
     4201 cgcaatctat cttgttggat acaggtgctt cattaacacg gcacattgca caaaaatcac
     4261 gcgctgatgc cgaaaaaaac agcgtttgga tgtcgaacat cggttatggc cgtgattatg
     4321 cttccgcaca atatcgccgg tttagttcga aacgcacgca aacacaaatc ggcattgacc
     4381 gcagcttgtc cgaaaatatg cagataggcg gcgtattgac ttactctgac agtcagcata
     4441 cttttgatca ggcgagcggc aaaaatactt ttgtgcaagc caacctttat ggtaagtatt
     4501 atttaaatga tgcttggtat gtggccggcg atattggtgc aggcagcttg agaagccggt
     4561 tacaaacaca gcaaaaagca aactttaacc gaacaagcat ccaaaccggc cttactttgg
     4621 gcaatacgct gaaaatcaat caattcgaga ttgtccccag tgcgggaatc cgttacagcc
     4681 gcctgtcatc tgcagattac aagttgggta acgacagtgt taaagtaagt tctatgtcag
     4741 tgaaaacact gacggccgga ctggattttg cttatcggtt taaagtcggc aaccttaccg
     4801 taaaaccctt gttatctgca gcttactttg ccaattatgg caaaggcggc gtgaatgtgg
     4861 gcggtaattc cttcgcctat aaagcagata atcaacagca atattcagca ggtgccgcgt
     4921 tactgtaccg taatgttaca ttaaacgtaa atggcagtat tacaaaagga aaacaattgg
     4981 aaaaacaaaa atccggacaa attaaaatac agattcgttt ctaaaatact aaattcatag
     5041 caaaataaaa tgccgtctga actcaggctt cagacggcat ttttatagtg gattaacaaa
     5101 aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg
     5161 ccgtactggt ttttgttaat ccactatatc agacggacga ggttacagtt taatcccttt
     5221 gagcttcgcg acggtattga tgtctttgtc gccacgaccg gataggttga ccagaatcac
     5281 ttggtctttg cccattttcg gcgcgttttt caccgcccaa gcaacggcgt ggctggattc
     5341 gagcgcgggg atgatgccct cgaagcggca gagcaaatca aaggcttcga gtgcttcgtc
     5401 gtctttagcg acggtgtatt cgacgcgctt gatgtcgtgc agatggctgt gttccgggcc
     5461 gatgccgggg taatccaagc ctgcggaaac agagtgtgta cccaagactt gaccgttttc
     5521 gtcctgcatc agatagctgc ggaaaccgtg caatacgcca atcggtgagc ccgaagtaat
     5581 cggcgcggcg tgatcggggg tgttcacgcc caaaccgcca gcctccacgc cgacgaggcg
     5641 cacgttttct tcgccgatat acgggtggaa caaaccaatg gcgttcgatc cgccgcccac
     5701 gcaggcaacg gcaacgtcgg gctgtctgcc gatggcttcc aacatttgtt ctttggcttc
     5761 attgccgatc acgcattgaa agtcgcgcac catttcggga tacggcgcgg gaccggcggc
     5821 ggtaccgatg atgtagaatg tgtcatccac gcgggcgacc cattcgcgca tggcttcgtt
     5881 catcgcgtct ttcaaagtac ggctgccgct gtcaacgccg acgacattcg cgcccaataa
     5941 tttcatacgg aacacgttgg gcatttggcg ttggatgtcg tccgcaccca tgtacacgtc
     6001 gcaagtcatg ccgaagcggg cggcaacagt ggcactcgcc acgccgtgct gacccgcgcc
     6061 ggtttcggcg atgacgcgtt ttttacccat acgttttgcc aacagtgcct gaccgatggt
     6121 gttgttgact ttgtgcgcgc cggtgtggtt caagtcttcg cgcttcaacc agatttgcgc
     6181 accgcccaaa tgctcggaca accgcgcggc gtggtaaacg gggctgggcc tgccgacata
     6241 atgtttcaaa tcgcggtgga attcttccca aaacgacggg tcgtttttcg ctgccttata
     6301 ggcatccgcc agctcttgca aagcgggaat cagggtctcg gacacgaaca gcccgccgtg
     6361 ttcgccgaaa aagcccttct cgtcgggcgc gtggtagttc ttcatcggat tcttccttgt
     6421 gcttcaggtt ttcaaacggc atttccgttt tttcaaacat gccgtccgaa cgggaaattg
     6481 tcggcaatta taactgcttt tcacacaagc ccgccatttc gaaaaacgcc cgatacaccg
     6541 ccgccttttt cgccaaactc aaaggatagg cgcgcgaagt cgaaggcagg cgcgtcagcg
     6601 tcaaatcccg tccggcaaac ggaaacggca cggtttcgcc cgttttcggc attttgatgc
     6661 cgccaccctg aatatcgagc aggatttccg tcgccttgcc gcctgtcgta caaatatggc
     6721 ggcactcggg tatttttgcc aacaccgccg ccaaatcgac ggtttcaact acctttaaaa
     6781 acttgtcgga cgcattgccg tgttcccttg ccgccttcaa cacggtcgga caggacgcaa
     6841 tcccccgttc gtgcaaaaac gccttgattt tctcggcatc aaacgctttt tcagacaacc
     6901 tttggaaatg cgccgcatca ttaaaaaaca ccagcccgta aacgcgccac atatcgtttt
     6961 ggaaattcgg ataatgaaac tgcatcgcgc gtttgtcttc cttgggcgga aacgtcccca
     7021 tcatcatgac cgcagccttc ggcggcaaca cggaatcgaa gggatgggtt tcgatttcaa
     7081 gcagattttc agacacgttc aatcctccaa caggcaaaac ggcagacagc cggtttttgc
     7141 accaaaccgc caaacacggc aagccttcaa acagcattat cacgccctgc cgttttcggt
     7201 tacaatgccg acatcaaacg gatactgtaa acacatttat gttccaacac acaggacgac
     7261 acttaaagca ccgccctatg tgccatcctg atttggaagg agttacgccc ctcccaaata
     7321 gagtctgatt ctaccgccct aaagggcggg gtttcaaccg aaaaggaaac acgatgaaag
     7381 aacacaaagc ccgcaagcgt ttcgggcaga attttttgca ggacacgcgg atcatcagcg
     7441 atattgtcaa cgccgtgcgc ccgcaggcgg atgatgtcgt gattgaaatc ggcccgggtt
     7501 tggcggcgat taccgaacct ttggcgaaaa agctgaaccg cctgcacgtt gtcgaaatcg
     7561 accgcgacat cgtatgccgt ctgaaaacgc tgccgtttgc ggataaactg gtgattcacg
     7621 aaggcgatgt attgcagttt gatttcaacg gcatcgcagg caaaaagaaa atcgtcggca
     7681 acctgcccta caacatttcc acgccgcttt tgttcaagct ggcggaggtg gcggacgatg
     7741 tcgtcgatat gcactttatg ctgcaaaaag aagtggtcga tcgtatggtt gccgcgccga
     7801 aaagcaacga ctacggccgc ttgggcgtga tgctgcaata tttcttcgat atggaaatgc
     7861 tgattgacgt gccgcccgaa tcgttcgacc ccgcgccgaa agtggattcg gccgtcgtcc
     7921 gcatgattcc ggtgaaacac cgtatcggca aggcggacga tttcgagcat ttcgccaaac
     7981 tcgtgaaact cgccttccac caacgccgca aaaccatacg caacaatctg aaagagcttg
     8041 caggcgacga tgatttgcag gcagtcggca tcaatccgca agaccgcgcc gaacatatcg
     8101 cgccggagaa atatgtggcg ttgagcaatt atctggcggg caaggccgtc tgaaaaggga
     8161 gtaagccgat gacgaaatac ctgcttttac ccctgatact gccgcttgca gcctgcctga
     8221 ccaatccgaa cgaccgtttt tttgacggca gacgctacac gcttcatcat atggagctcc
     8281 cgtaacgaca acctgcaacg gcaaatgccg tctgaaagaa aagacatgat caaattcaaa
     8341 aacgtacaca aacatttcaa agacctgcac gtcatcaacg gcgtgaactt ggaaatcaaa
     8401 aaaggcgaag tggtcgtcgt ctgcggacct tcgggcagcg gcaaatccac actcatccgc
     8461 accgtcaacc agcttgaaag catcgaaagc ggcgagattt gggtggacgg tgtcaacgtt
     8521 gccgacccaa aaaccgattt gaacaaaatc cgcgaagaag tcggctttgt gtttcaaagt
     8581 ttcaacctct acccccacct gaccgtattg gaaaacatca ccctcgcacc gatgaaggtt
     8641 aaaggtcaaa atgcggaaca ggcggagaaa aaagcgatgg aacttttgga acgcgtcgga
     8701 ctggcgcaca aaaaagacgc ttttccgtcc caactttccg gcggccagca gcagcgcgtg
     8761 gcgattgcac gcggtttggc gatggaaccg cgcgtaatgt tgtttgacga accgacctcc
     8821 gcactcgacc ccgaaatggt cggcgaagtg ttgaaagtga tgaaagactt ggcagaaagc
     8881 ggcatgacca tgatgtgcgt aacccacgaa atggggtttg cccgcgaagt tgccgaccgc
     8941 gtgattttcg tcgataaagg gcaaatcctc gaagacgaaa cgcccgaagc gtttttcacc
     9001 aacccgaaac acgaacgcgc caagcagttc ctgcaacagg tcatgaccca ctgatgctat
     9061 accgtctgaa aaccgaacgt tcagacggca tagcccgaca ccgcaagatg attgagaaag
     9121 aaaaccatgt attttgttga ccgcaccgcc gtcgtcctca agccgaccgt ccgctttttg
     9181 gaatggctca aaagcaccga cgaaaatatg cccgacctga ccatagagca gcttcgtgcc
     9241 aactgctccg tcttcctcgt tccgcagttc gacgaaccgg aagccgtcgt ttcctatttc
     9301 gacgaacgct accgccagat ttttgaagcc gaactcgccg gctgggacat cgacaaggat
     9361 aaatggccgc aagatatggg gctgaaggcg ttttgggaat ttttcgacat cgaaatccac
     9421 gatatggtgt tggatatgga agaagcggat ttgaacatca cgcccgtgtt cgacaatatg
     9481 atgtaacgcc gtgcgcgcct ttcagacagc attgaggccg tcccgcatcc tgaacatcct
     9541 gaccgtatcg ctccactcgg cttcccttgc cgtctgcctg acctggtttt acggccggat
     9601 gatgtggttc ggtttggcgg ctttggcggc aagttacgca tactcgctca agataacgaa
     9661 tctgaaacac cgccatgcca taaccgccat taccatagac cgcgacggac aggcggaaat
     9721 cgtatccggc aaagaaaaaa cagcaaaggc ggcaacactt gcgggcagca gtatggttac
     9781 gccttatgcc ttattcttac aatgggacac cggcggcaaa accgtccgcc actgcatcat
     9841 gcccgacatg acggacaaag aatcctaccg caagctgaaa gtctgggtac tttggcggca
     9901 gccgaaaaaa accgccgaaa ccgatacatc agactgattt cagacggcat ttgaaaacga
     9961 aacactcatg aaaacactac aagactggct ctcgcatttg gaaaccgccc acagcggcgg
    10021 tttgatcgac atgggtttgg agcgcacgag cgaagtgaaa aaacgcatga agctcgagcc
    10081 gcaatgcccc gtcgtcgttg tcgcgggaac caacggcaaa ggttcggtct gtgcctacct
    10141 gacacagatt tacaaacagg cgggctacaa aaccggtacg ctgaccagcc cgcatttatt
    10201 gcgttacaac gaacgcatcg ccatcaacgc cgaacccgtt tcggacgata cgattattgc
    10261 ctctttcgag cgcatcgaag cggcgcgcag cgaaatatcg ctgacttatt ttgaattcaa
    10321 tacgttggcg gccgtcgaca tcttcatgcg cgaacaggtt gacgtgatga tattggaagt
    10381 cggcttgggc ggacgcttgg acgcggtcaa cgcatttgac ggcgattgcg cggtggttac
    10441 tagtgtagat ttggaccatc aggcgttttt gggcgatacg gtcgagcagg tcggctttga
    10501 aaaagcaggc gtgttccgca gcggcaaacc cgcaatctgc ggtcaaaacc ccgcgcccga
    10561 atcgttgctt tcacacgccg aagccatagg cgcgaaactg ctgatggtgc agcgcgattt
    10621 cgagtttcac gcgatggaaa acatccaatg gaactaccgc ttccggccgc aacattcaga
    10681 cgaccccgcg cgcaaccgca atgccctgcc cttccccgca ttgcgcggca cataccaact
    10741 ttccaacgcc gcctgcacgt tgaccgtatt ggaatgcttg aacgccaaat tgccggtgga
    10801 catcggcgcc atcaaacgcg gattgctgct ggttgaaaat cccggacgct tccaagtcct
    10861 gcccggccgt ccgctgaccg ttttggatgt cggacacaat ccccacgctg cccgcgccct
    10921 gcgccgcagc ctgattaatt tggcgtttgc gcaaaaacgc accgccgtgt tcagcatgtt
    10981 gtccgacaaa gacatagacg gcgtgttgga aaccgttaaa gaccagtttg acgagtggta
    11041 tatcgcaccg ttggatgtac cgcgcggcat gacgctcgag agcctgcaaa acaaactgca
    11101 cgaacacagc atcgaaaaca tacaaacatt cgccgccgtc cgcgaagcat accgggcggc
    11161 agcctcaaaa gcgggcgaga acgacagaat cgtcgttttc ggctcatttc ataccgttgc
    11221 cgatgtcatg tcggtacttt aaaaggaaac ttctatgtcc gaaaacaaac aaaacgaagt
    11281 cctgagcggt tacgaacaac tcaaacggcg caaccgccgc cgcctcgtaa cggcaagttg
    11341 cctggttgcc gcctcctgca tcctgctggc agccgccctc agttccggcc ctgccgaaca
    11401 gactgccggc gaaacaagcg gcgtagaaaa caaagcggca ggtgcggcac aaacccctgc
    11461 cttgaaatcc gccgccgaca aaccgcagga cttggcaggc gaagacaagc cttctgccgc
    11521 cgacagcgaa atcagcgagc ctgaaaacgt aggcgcgccg ctggtgctga ttaacgaccg
    11581 cctcgaagac agcaacatca aaggtttgga agcatccgag aaactgcaac aggcagaaac
    11641 cgccaaaacc gcaccgaagc aggcaaaaca acgcgctgcc gaaaaagtgc cggcaactgc
    11701 cgacagtacg gatacggtag cggttgaaaa accgaaacgc actgccgaaa caaaaccgca
    11761 aaaagcggaa cgcactgcca aagccaagcc caaagccaaa gaaaccaaaa ccgccgaaaa
    11821 agttgccgac aaaccgaaaa ctgccgccga aaaaaccaaa ccggatacgg caaaatccga
    11881 cagcgcggta aaagaagcga aaaaagccga caaggctgaa agcagaaaaa cagccgaaaa
    11941 agaccgttcg gacggcaaaa aacacgaaac ggcacaaaaa accgacaaag cggacaagac
    12001 caaaaccgcc gagaaggaaa aatccggtaa aaaagccgcc attcaggcag gttatgccga
    12061 aaaagaacgc gccttaagcc tccagcgcaa aatgaaggcg gtgggtatcg attcgaccat
    12121 caccgaaatt atgaccgaca acggcaaagt ttaccgcgtc aaatcaagca actataaaaa
    12181 cgcaagggat gccgaacgcg atttgaacaa attgcgtgta cacggtatcg ccggtcaggt
    12241 aacgaatgaa taacctcccc gttgccgacc tcctcgtctt cgccatcatc gccgcctgca
    12301 tcgtgctatc cgcgatgcgc ggcgtgattg cggaggcagg ttcgatggcg gcatgggtgg
    12361 tttccttctt ttttgccaaa ctctttgccg cacccttcgc cgacctcgcc tttgcctcgt
    12421 tccaaccccg cctgtttgca ttggctctgt cgttcatttc cctgttcgtc attgcctgtc
    12481 tgatccagaa aatgctccgt tcgctgctga ccggcgcagt ttcggcggtc ggtttgggct
    12541 ttgccaaccg cattttgggc ggcgtattcg gtgcattgaa aggcgttttg attgttaccc
    12601 tgctggtcat gcttgcttca aaaaccgacc tgcccgatac cgaagagtgg cagcagtcct
    12661 ataccatacc gtttttcgta tcgctttccg aagccgtgtt gaaccatagc ggcggcacgg
    12721 cggaaactcc ggaagacgat tgattgccat acggatattt cagacggcat tctcttttat
    12781 gccgtctgaa atgttttttc cttttctcag ccatatcaca gattaaatct tatcctccat
    12841 aaacccacct atctgtcctt cgctcaaata ttgtcaacaa ttcaggcaaa aaaccaccgt
    12901 ttgctttata attccgtttt acccttaaac ctcgaatcgg agaagacgat atgtgcggcg
    12961 tattaggttt ggtcagtcat gaacccgtga accagcttct gtacgacggt ttgcagatgt
    13021 tgcagcacag ggggcaggat gcggcgggca ttgcaacggc ggaaggcggc accttccata
    13081 tgcacaaagg caaagggatg gtgcgcgaag tgttccgcac gcgcaatatg cgcgatttgg
    13141 tcggcaacgc cggcatcgcc cacgtccgtt atcctacggc gggcaacgcc ggcagcagcg
    13201 cggaggcgca gcctttctac gtcagctcgc ctttcggcat cgttttggcg cataacggca
    13261 atctcaccaa cacggccgaa ctgtatgaaa acgtgtgcaa caaacacctg cgccacgtca
    13321 acaccagctc cgattccgaa gtcttgctca acgtattcgc gcacgaatta cgccgcgaag
    13381 tctccaaaaa cgccgatccg caccggctca atgccgacaa tattttcaac gccgttgccg
    13441 aagtccaccg cctagtgcgc ggcgcatacg gcgtggttgc catgattgcg ggctacggta
    13501 tgcttgcctt ccgcgaccct tacggcatcc gcccgctggt attgggttcg caaaccgaca
    13561 acgaaggcag gaaatcctat gccgtcgcct ccgaatccgt cgccttcaac gcgcttgcct
    13621 acgatttgga acgcgacatc cggccgggcg aagcggtatt tgtcggcttt gacggcacaa
    13681 tgattgcccg ccaatgcagc gaccgtgcca aactcagccc ctgccttttt gaatatgttt
    13741 attttgcccg ccccgactcc gtaatcgacg gcgtatcggt ttaccaatcg cgcttggata
    13801 tgggcgtgtc cttggcggaa aaaatcaaac gcgagctgcc cgtggacgac atcgatgtcg
    13861 tgatgcccat tcccgacacc agccgcccca gcgcgatgga gcttgccgtc cacctcgaca
    13921 aaccctaccg cgagggcttg attaaaaacc gctatatcgg ccgcaccttt attatgcccg
    13981 gccaggcgac gcgcaaaaaa tccgtgcgcc aaaaactcag cccgatggaa accgagtttg
    14041 caggcaaaag cgtgttgctg gtggacgact ccatcgtgcg cgggacgacc agccgcgaaa
    14101 tcgtcgaaat ggtacgcgcg gcgggcgcgc gcaaagtcta tatcgcctcc gccgcccccg
    14161 aagtgcgcta tcccaacgta tacggcatcg atatgcccac gcgcgaagag ttgattgcca
    14221 acgggcgcag cgcggcggaa atcgccgccg aaatcggcgc ggacggcatc gtgttccaaa
    14281 atttgagcga tttggaagcc gtcgtcaaag cactcaatcc gaaaatcgaa tccttcgatt
    14341 cgtcctgttt caacggcatt tatcagaccg gcgacatcga tgatgcctac ctcgaccgcc
    14401 tgtctgccga gaaatccggc tgcggcggcc tgaaaatcca cccgagtagg atggaacaca
    14461 gcatcagcat cagcgatgcg ggcgacgaag aataaggctt cccaaaccgc gatgccgtct
    14521 gaaatccgaa cgccgggttt tcagacggca tttcccatat cggcatcaca tttcccattt
    14581 catccgcacg caatatgagt accgaaacca aaaactacat tacgcccgcc ggatggcagg
    14641 cattgaaaga cgagctttat caactggtta ataaagaacg ccccgaaatc gtccaaatcg
    14701 tcaactgggc ggcaggcaac ggcgaccgca gcgaaaacgg cgactatctt tacggcaaac
    14761 gccgtatgcg cgaaatcgac cgccgcatcc gtttcctgac caagcgtttg gagtccgccg
    14821 tcgtcgtcga tcccgaattg cgcgaagcga ccgaccaagt atttttcggc gcgacggtcg
    14881 ggctgttgcg cggcgacggg cgcgagcaaa ccgtcaaaat tgtcggcatc gatgaaatcg
    14941 ataccgcgca aaacaaaatt tcttggattt cgccgttggc gcgttgcctg attaaagcgc
    15001 gcgaaggcga cgaagtggtt ttgaacacgc cggaagggcg tgaggaaatt gaaatccttt
    15061 ctgtcgaata catcaaaatc gactgatcgg gaatgccgtc tgaaacggca caaacgcatc
    15121 gtttcccaaa taaaggaata ccgaatgagg aaaatccgca ccaaaatctg cggcatcacc
    15181 acactggaag acgcagcggc cgccgcagcg gcaggtgcgg atgccgtcgg gctggtcttt
    15241 ttccaaggca gcagccgggc cgtcgatatt gcccgcgcca aaaaaatcac cgccgcactg
    15301 ccgccgtttg tcagcgttgt cgcccttttc gtcaacgaaa gcgcgcaaaa catccgccgc
    15361 atccttgccg aagtgccgat acacatcatc caattccacg gcgacgaaga cgacgcattc
    15421 tgccgccagt tcgaccgccc ctatatcaag gccattcgtg ttcagacggc atcagacatc
    15481 cgaaacgccg ccgaccgctt ccccgacgct caggcactgc tgttcgatgc ctaccaccct
    15541 tcggaatacg gcggcaccgg acaccgcttc gactggacgc tgctggcgga atattcgggc
    15601 aaaccgtggg tgcttgccgg cgggctgacc cctgaaaacg tcggcgaagc cgtccgcatc
    15661 accggagcgg aagcggtcga tgtatccggc ggcgtggaag cgtctaaagg caaaaaagac
    15721 ccagccaaag ttgccgcctt tatcgcaacc gccaaccgcc tatcccgtta aagcaacaaa
    15781 aattgccgcc ggaatga
//
LOCUS       DAXHTR010000035        15744 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.035, whole genome shotgun sequence.
ACCESSION   DAXHTR010000035 DAXHTR010000000
VERSION     DAXHTR010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15744
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.035"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            124..856
                     /locus_tag="ACFNML_000761"
                     /pseudo
     CDS             124..856
                     /locus_tag="ACFNML_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
     gene            972..2480
                     /gene="ppx"
                     /locus_tag="ACFNML_000762"
     CDS             972..2480
                     /gene="ppx"
                     /locus_tag="ACFNML_000762"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFC6392174.1"
                     /translation="MTTHPANVLASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSAASQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDRMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEAV"
     gene            complement(2689..3117)
                     /locus_tag="ACFNML_000763"
     CDS             complement(2689..3117)
                     /locus_tag="ACFNML_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392175.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAKEAVTEAKEAVTEAKEAVT
                     EAKEAVTEAKEAVTEAAKDTLNKAADATQEAADKMKDAAK"
     gene            complement(3437..3778)
                     /locus_tag="ACFNML_000764"
     CDS             complement(3437..3778)
                     /locus_tag="ACFNML_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392176.1"
                     /translation="MPSEPLKGLPEQNVVRLTGKHPNDLEAVSGKCMETDDKGGPVGW
                     AENGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQNGRYVLEIDS
                     EGAFYFRRRHY"
     gene            complement(3784..4794)
                     /gene="trpS"
                     /locus_tag="ACFNML_000765"
     CDS             complement(3784..4794)
                     /gene="trpS"
                     /locus_tag="ACFNML_000765"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFC6392177.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANAENGQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAQKARKEARELLDKVRDAVGIRP
                     LK"
     gene            5055..7634
                     /gene="clpB"
                     /locus_tag="ACFNML_000766"
     CDS             5055..7634
                     /gene="clpB"
                     /locus_tag="ACFNML_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFC6392178.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADSSYLEAGFVLKALLDDQNS
                     GAAALLAHAGVNVPQVKQRLQQHLNSLPKVSGQGGDILPSRELQAVLNLMDKAATKRG
                     DAYIASELFLLALVQQNDAAGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEIDG
                     LEKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            complement(7721..8935)
                     /locus_tag="ACFNML_000767"
     CDS             complement(7721..8935)
                     /locus_tag="ACFNML_000767"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HFC6392179.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKAYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVNQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(9024..9233)
                     /locus_tag="ACFNML_000768"
     CDS             complement(9024..9233)
                     /locus_tag="ACFNML_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HFC6392180.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLAHVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            9578..10384
                     /locus_tag="ACFNML_000769"
     CDS             9578..10384
                     /locus_tag="ACFNML_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HFC6392181.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            complement(10442..11707)
                     /locus_tag="ACFNML_000770"
     CDS             complement(10442..11707)
                     /locus_tag="ACFNML_000770"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HFC6392182.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKIVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            12114..12821
                     /locus_tag="ACFNML_000771"
     CDS             12114..12821
                     /locus_tag="ACFNML_000771"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFC6392183.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLSNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAAELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            12878..13339
                     /gene="recX"
                     /locus_tag="ACFNML_000772"
     CDS             12878..13339
                     /gene="recX"
                     /locus_tag="ACFNML_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFC6392184.1"
                     /translation="MKPQKSLRARAMDILSRQEVSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKQAA
                     IDVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            13413..13574
                     /locus_tag="ACFNML_000773"
     CDS             13413..13574
                     /locus_tag="ACFNML_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392185.1"
                     /translation="MSEQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWAGVLALVF
                     FLVFRFWLS"
     gene            13727..14209
                     /locus_tag="ACFNML_000774"
     CDS             13727..14209
                     /locus_tag="ACFNML_000774"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6392186.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EIRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(14416..15663)
                     /locus_tag="ACFNML_000775"
     CDS             complement(14416..15663)
                     /locus_tag="ACFNML_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6392187.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKTAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
BASE COUNT         3929 a         4285 c         3961 g         3569 t
ORIGIN      
        1 ttttgttaat ccgctataaa caacgcttcg tccaaaaaaa cgattgaatt tgcgggcaga
       61 agctggacga aaaccgccga cagcctgccg caaaaggcac acggtttgcg ctagggctta
      121 ggcgtgtcgc gcgaaatcaa tgcgggcagg catcatttcc tctacggcgg catcagcggc
      181 ggcggcgtgc attattggga taacaaagat ttcagcgaac agagcctgcg cctgtcgttc
      241 ggctataaaa accgttcggt aacgcgctcg ttcggcatcg tgccgtttgt cgagcaaaac
      301 ctcttaggcg gcagccgata caatttcgtc ggcggcttta atgccgattt ctcccaacac
      361 ttgagcgaac gctggcggtt gacactaaac gcgggcaata tgtggaagca ttatcaggaa
      421 gaccgcaccg ccgcccggta cgacagccat atgccgctgg cgggcgcgac gctgatgtat
      481 tccgcgccga aagactggct gctttacggc ggggcggact ggtcgcacga cttgacgaag
      541 gaggcggaac aggcttccgt ccgcaagggt ttgcgtgtcg gcgcagtcaa aacgttcgac
      601 ggcgggcttg ggtctgcggg caaacctgcg ctatacccgc aggatgtttg acgcacccgg
      661 gaccattgtg taccgcttcc cgcgcaaaga ccacgaatat caggcaaacc tgtcgttgtg
      721 gcacgacaaa atctcttgga agggctttac gccgcaactc aatttccgct atctgaaaat
      781 cgacagcaat atgaaaagtt tttacacacg caaaaacacg cagattttca tgagcgtgga
      841 aaaggatttc aaataagcgc aaaaatgccg tcggcaacat ccgtgggcag aatcaaaaac
      901 cgccgcatca tttattgtca acgcctgcgc cgtcagagta acattgcgtt tttcccccac
      961 cggtatccac catgaccacc catcccgcaa acgtcctcgc ctccgtcgat ttgggttcca
     1021 acagtttccg cctccagatt tgcgaaaaca acaacggaca attaaaagtt atcgattcgt
     1081 tcaaacagat ggtgcgcttc gccgccggtc tggacgaaca gaaaaacctg agtgccgctt
     1141 cccaagaaca ggctttggac tgcctggcaa aattcggcga acgcctgcgc ggcttccgcc
     1201 ctgaacaggt gcgcgccgtg gcaaccaata cattccgcgt cgccaaaaac atcgcagatt
     1261 tccttcccaa ggccgaagcg gcactgggtt tccccatcga aatcatcgca gggcgcgaag
     1321 aggcgcggct gatttatacc ggcgtgatcc acaccctccc cccgggcggc ggcaaaatgc
     1381 tggttatcga catcgggggc ggttcgacag aatttgtcat cggctcgacg ctgaatcccg
     1441 acattaccga aagcctgccc ttgggctgcg taacctacag cctgcgcttc ttccaaaaca
     1501 aaatcaccgc caaagacttc caatctgcca tttccgccgc ccgcaacgaa atccagcgca
     1561 tcagcaaaaa tatgaggcgc gaaggttggg atttcgccgt cggcacatcg ggttcggcaa
     1621 aatccatccg cgacgtgctt gccgccgaaa tgccccaaga ggcggacatt acctacaaag
     1681 gcatgcgcgc cctcgccgaa cgcatcatcg aagccggttc ggtcaaaaag gccaaatttg
     1741 aaaacctgaa accggaacgc atcgaagttt ttgccggcgg acttgccgtg atgatggcgg
     1801 cgtttgaaga aatgaaactc gacaggatga ccgtaaccga agccgccctg cgcgacggcg
     1861 tgttttacga tttgatcggg cgcggtttaa acgaagatat gcgcggacaa acggttgccg
     1921 agttccaaca ccgctaccac gtcagcctca atcaggcgaa acgcaccgcc gagaccgcgc
     1981 aaacctttat ggacagcctc tgccacgcta aaaatgttac ggttcaagag cttgccttgt
     2041 ggcaacagta tctcggacgc gccgccgcgc tgcacgaaat cggtttggac atcgcccaca
     2101 ccggctatca caagcattcc gcctacatcc tcgaaaacgc cgatatgccg ggtttctcac
     2161 gcaaagaaca gaccatactt gcccaactgg tcatcggtca tcgcggcgat atgaaaaaaa
     2221 tgagcggcat catcggcacc aacgaaatgt tgtggtatgc cgttttgtcg ctgcgccttg
     2281 ccgcactgtt ctgccgttcg cgccaagacc tgtctttccc gaaaaatatg cagttgcgta
     2341 cggacacgga aagctgcggc ttcatcctgc gtattgacag ggaatggctg gaacgccatc
     2401 ccctgattgc cgacgcattg gaatatgaaa gcgtccaatg gcaaaaaatc aatatgccgt
     2461 tcaaagtcga ggccgtctga accttgcggg gcaaatgccg tctgaatgat gttcagacgg
     2521 catttccctg tccgcaacat cccgatatgc gcggcacatc tgctcggaac ggtcatgcag
     2581 gcgtaaaaaa caaggggcat acaactcaaa aaccgcctga aaatcttcag gcggtttcgt
     2641 ttgggttgcc ggcaggcggc atcccatcat ttttgccaag gcaacaaatt atttggcggc
     2701 atctttcatt ttgtctgccg cttcctgagt cgcgtcggca gctttgttca aagtatcttt
     2761 agctgcttca gttacagctt ctttggcttc agttacagct tctttggctt cagttacagc
     2821 ttctttggct tcagttacag cttctttggc ttcagttaca gcttctttgg cttcagttac
     2881 agcttctttg gcttcagtta cagcttcctc ggcacttgcc tttgcatcag ccgcagcatc
     2941 tttgacttgg tctttcgctt cttcgacggc agaagcggca gactcggcgg cagaagccgc
     3001 agtgtcttta acatcggact caacggcttg aaccgcttcc ttaacctcct gtttggcttc
     3061 ttgcgaacaa gctgccaagg cagccgccat cattgcggca atcaataatt ttttcatgtc
     3121 ttatccttct tgagttgttg attaaggttt tgcttaaaaa tcgaaccgtg ttccatcaat
     3181 cggctgattt tgcccatcgg ccggagagaa aacggtttcc cgtttagtta aaacccatta
     3241 tatttaaata taaaggtttt tttctcgaac aataaggcgg catcaatgcc atattgaaac
     3301 acgtccgaaa actattttat gaaaacagtt cggaaaattg taacacatat ccccctcctt
     3361 ttgagtttcc ctgcggtgcg gacttttttt cgcaaggttt gaaaaaccca aatatattcc
     3421 gggatgtccg aatacctcaa taatggcggc ggcggaaata aaacgccccc tcgctgtcga
     3481 tttccagcac atagcgtccg ttctgcacgg cggcatagcc gcttttgcct gcctgatagg
     3541 gttgcagggc ggaatgcgaa atcaggtaat ccgtcagttt gccgccgtct tcggcgatat
     3601 tgcccaccag tttggcaaac aaggtatggc acacgccgtt ttccgcccag cctaccggac
     3661 cgcccttatc gtcggtttcc atgcatttgc cgctgacggc ttccaagtcg ttgggatgct
     3721 tgccggtcag ccggacgacg ttttgttccg gcaagccttt aagaggttcg gacggcatcg
     3781 ggtttatttc aacgggcgga tgccgaccgc atcgcgtact ttgtccaaca attcgcgtgc
     3841 ttctttacgc gctttttgcg cacctgcctg taaaatctct tcgatttgcg aagggtcggc
     3901 ggtcagctcg ttgtagcgtt cgcgcggttc ggcgagttcg gcgttgattt tcgccgccaa
     3961 aagttttttt gcctcgcccc aagccaagcc ttcggcaagc atttgcgtga acgctgcggt
     4021 ttcagacggc gtggagaagg ctttgtagat ttcaaacaat gggctttcgt cgggctgttt
     4081 cggctcgccc ggctctttca tattggtgat gattttgttg accgattttt gggttttttt
     4141 gtcgttttcc caaagcggaa tggtgttgcc gtaggatttg gacattttgc gtccgtccaa
     4201 accgaccaag agttcgacgt tttcgtcgat tttcacttcg ggcagggtaa agatttcttt
     4261 aaagcggtga ttgaagcggc cggcgatgtc gcgcgccatt tcgacgtgtt ggatttggtc
     4321 gcgcccgacg ggcacttcgt tggcgttgaa cataaggatg tcggcggtca tcagaatcgg
     4381 atagctgaac aaacccattt ccacgccgaa atcggggtct tcctgcccgt tttccgcatt
     4441 tgcctgcacg gcggctttgt aggcgtgggc gcggttcatc aaacccttgg cggtgatgca
     4501 ggtcagaatc cagttcaatt ccatcacttc gggaatgtcg ctttggcggt agaaggtggt
     4561 gcgctcgggg tcgagtccgc aggcaagcca agtggcggca acggcttggg tggattggtg
     4621 aatcatctcc ggctcgtggc atttgatgat accgtggtaa tcggcgagga agaggaagga
     4681 ttcggtatca gggttttgcg ccgcgcggac ggcggggcgg atagcaccga cgtagttgcc
     4741 cagatgcggg atgccggtgg tggttacgcc ggtcagaact cgttttttgc tcataaaaat
     4801 gtccttgcgg catcaatgcc gtctgaaagg gaaaaagatg cgccgattat acccgatttg
     4861 ccacctacat ccagccgaca acagactttt ccatattaag aagatatagt tatacacatt
     4921 attatacatt tttatatact ttaaattcaa tgatatatcg aattaaatat agaaaaacag
     4981 aaaacagaac ttgagttatc cacaattatg cacatatagg cttcgacagc ggacattttg
     5041 aaaaggaaac aaaaatgcga tacgacaaat taaccgccaa attccaacag gcccttgcag
     5101 aagctcagag tttggcgttg gctgcggaca gcagctatct ggaagcgggc tttgtgctga
     5161 aagccctgct tgacgaccaa aacagcggtg ctgccgcgct cttggctcat gcgggcgtga
     5221 acgtgccgca ggtgaaacag cgtttgcagc agcatttgaa cagcctgccg aaagtgtccg
     5281 gccagggcgg cgacattctg cccagccgcg aattgcaggc ggtgttgaac ctgatggaca
     5341 aagccgcaac caaacgcggc gatgcctata tcgccagtga acttttcctg cttgccttgg
     5401 tgcagcagaa cgatgcggcc ggcaaaattt tgaaagaagc cggcgcgacc gaacaaaaca
     5461 tcaatgccgc gattgacgcg gtacgaggag gacaaaacgt gaacgatgcc aatgccgaag
     5521 accaacgcga tgctttgaaa aaatatacgc tcgatctgac ccaacgcgcc cgagacggta
     5581 agctcgaccc cgttatcggc cgtgacgacg aaatccgccg tgcgattcag gtattgcaac
     5641 gccgtaccaa aaacaaccct gtgctgattg gcgaaccggg cgtgggtaaa accgccatcg
     5701 tcgaaggttt ggcacaacgc atcgtcaacg gcgaagtacc cgaatccctg cgcaacaaac
     5761 gcctactggt tttggacttg gcggcattga ttgccggcgc gaaataccgc ggcgaatttg
     5821 aagaacgctt gaaaggcgtg ttgaacgact tggcgaaaga cgacggcaac actctgattt
     5881 tcatcgacga aatccatact ttggtcggcg caggcaaaac cgacggtgcg atggatgcgg
     5941 gcaatatgct gaaaccggct ttggcacgtg gcgaattgca ctgtatcggc gcgaccacct
     6001 tggacgaata ccgccaatac atcgaaaaag acgcggcact cgaacgccgc ttccaaaaag
     6061 tattggttgg cgagccaagt gtagaagaca ccatcgctat cttgcgcggt ttacaggagc
     6121 gttatgaaat ccaccacggt atcgatatta ccgaccctgc tatcgttgcc gcagcggagt
     6181 tgagcgaccg ctacattacc gaccgcttcc tgcctgataa agcgattgat ttgattgacg
     6241 aagccgccag ccgtgtcaag atggaaaaag aaaccaagcc ggaagcaatg gacaaaatcg
     6301 accgccgtct gattcagctt cggatggaaa aggcgcacgt tgaaaaagaa aaagacgatg
     6361 ccagcaaaaa acgtttggaa ctgatagatg aggaaatcga cggtttggaa aaagaatacg
     6421 ccgatttgga cgaaatctgg aaagccgaaa aagcgatttc agacggtgct gctaatatta
     6481 agaaacaaat tgacgaagtc aaaattaaaa tcgaacaggc aaaacggcaa ggcgatttgg
     6541 cactggcttc aaaattgatg tatgaagatt tggagcattt ggaaaaacag cgtgcagccg
     6601 ccgaacgggc agatacggac agcacaaaac cggcaaacaa actcttgcgt aataatgtcg
     6661 gcgcagagga aatcgcagaa gttgtttccc gtatgaccgg cattcctgtg tcaaaaatga
     6721 tggaaggcga acgcgacaag ttgctgaaaa tggaagaagt gttgcaccgc cgcgtagtcg
     6781 gtcaagacga agccgtgcgt gccgtgtccg acgctatccg ccgcagccgt tccggtcttg
     6841 ccgacccgaa taaaccctac ggcagcttcc tgttcctagg cccgaccggc gtgggtaaaa
     6901 ccgagttgtg taaggcattg gcaggcttcc tgtttgacag tgaagatcat ctgattcgta
     6961 tcgatatgtc cgaatatatg gaaaaacacg ccgttgcccg cctcatcggc gcgcctccgg
     7021 gctatgtcgg ctacgaagaa ggcggctacc tgaccgaaca agtccgccgc aaaccgtaca
     7081 gcgtgattct tttggacgaa gtggaaaaag cccatcccga cgtgttcaac atcctgctgc
     7141 aagtattgga tgacggtcgt ttgaccgacg ggcaaggcag gacggtagat tttaaaaata
     7201 ccgtgattgt gatgacttcc aacatcggca gccagcacat ccaacaaatg ggcattcagg
     7261 attacgaagc ggtaaaagaa gttgtgatgg aggatgtgaa agaacatttc cgtccagaaa
     7321 tgatcaaccg catcgacgaa gtagtcgtgt tccacggact ggatcaggca aacatccgca
     7381 gcattgcgaa aatccagctc aaaggtttgg aaaaacgttt ggaaaaacaa aacctgcgcc
     7441 tgactgtttc cgatgccgca ctggacatca tcgccaaagc cggtttcgac ccgatttacg
     7501 gcgcacgtcc gctcaaacgc gccatccagt cggaaatcga aaacccgctg gcaaaagccc
     7561 tgcttgccgg aaactatgcg cccgaaagcg aaatcagggt agaagccgac ggcgacagac
     7621 tgaaatttgc ctgattcgtt cctgctgttg aaaatgccgt ctgaaacggg aatctccgtt
     7681 tcagacggca ttttttatcc tcggcagaca aaccgtcccc ttattggcgg taggtttgca
     7741 ggaatcttgc cagcctgccc atcgcctctt caatctgatg gacgtaaggc agcgtgacaa
     7801 tgcggaaatg gtcgggcttg atccaattaa accccgttcc ctgcaccagc aagacttttt
     7861 cgcgcaccag caaatcgtaa acgaatttca tgtcatcgtg gatacggtac atttcggtat
     7921 cgatttttgg gaacatatac atcgcgccca tcggtttgac gcaggatacg ccgggtatct
     7981 gattgaccaa ttcccacgcc ctgttacgct gttccaaaag ccgtccgccg ggcaaaatga
     8041 attcgttaat gctctgataa ccgcccaatg ccgtctgaat cgcatgctgc atcggcgtat
     8101 tcgcacacaa gcgcatggac gagagcatat ccaaaccctc gatgtaacct tttgcatgat
     8161 gtttcggccc gttgagcacc atccagcctt ggcggaatcc ggctacacgg taggctttgg
     8221 acaaaccgtt gaacgttacc gtcaaaaggt cgggggcgag cgcggcgatg tggtggtgaa
     8281 ccgcgccgtc ataaaggatt ttatcgtaaa tctcgtcggc gaaaataatc aaaccgtgct
     8341 tgcgcgccag ttcggcgatt tccaacagga tttccctgct gtacaccgcg cctgtcggat
     8401 tattgggatt gatgacgacg atggctttag ttttgggcgt gattttggct tccatatcgg
     8461 caaggttggg gaaccagccg ttttcttcgt cgcacagata atggcgtacc gtaccgcccg
     8521 caagcgttgc cgccgccgtc cacaaagggt agtcgggcgc gggaatcagg atttcgtcgc
     8581 cgtcgttgag caatgcctgc atagacatcg taatcagctc ggacacgccg ttgccgatat
     8641 agacatcatc aaccgtaata tcgcgcaaac ctttggtctg atagtagtga acaatggctt
     8701 tgcgggcgga atacagccct ttggaatcgc aatagccttg cgaagtcggc aggttgcgga
     8761 tgacatcgac caagatttca tcaggggctt caaagccgaa cggcgcaggg ttgccgatat
     8821 tgagtttaag gattttattg ccctcctctt ccaactgaag ggcttttttg tgaaccggcc
     8881 cgcgtatgtc gtaacagacg tgatcgagct ttgcagactt gggaaattta tccatgatgt
     8941 gttccgtaaa ttttgggcaa tgggtgggaa tgtactcttt ttcacgcgga atttaaagca
     9001 tcaaaccgag attttcaggc tttttacctg ccctctttgc gccgttcgct gacgcttttg
     9061 ccgcctattc cccagttatc ggtatccact tcgtcaatca cgacaaccgt tgtttcggga
     9121 tttttaccca gcacgtgtgc cagcaattcg gttacgccgc cgatcagttc cgctttttgc
     9181 gcggcagtcg gtgcttcctt gccgccggtt actttaatat tgacataagg catgatcttt
     9241 ctccgttttg aaatattgct atcttatcaa acaagttgcc tccgcccaaa cgtccgcttc
     9301 attttctgaa aaattcaaat cgatatagcg gattaacaaa aatcaggaca aggcggcgaa
     9361 gccgaagaca gtacaaatag cacggaaccg attcacttgg tgcttcagca ccttagagaa
     9421 tcgttctctt tgagctaagg cgagccaacg ccgtactggt ttttgttaat ccactataca
     9481 aaaagacagt tttcagacag caaatccgtc ttcacacgat acctattttg ttataacata
     9541 acaaaatctt taacccacac gagacaaagg ctgcaccatg aagaaaacat tgacactgct
     9601 cgccgtttcc gccctatttg ccacatccgc ccacgcccac cgcgtctggg tcgaaaccgc
     9661 ccacacgcac ggcggcgaat accttaaagc cgacttgggc tacggcgaat ttcccgaact
     9721 cgaacccatc gccaaagacc gcctgcacat cttcagcaaa ccgatgcagc tggttaccga
     9781 aaaaggcaag gaaaacatga ttcaacgcgg cacatacaac taccagtacc gaagcaaccg
     9841 tcccgttaag gacggcagtt acctcgtcat cgccgaatat cagcctactt tctggtcaaa
     9901 aaacaaagca ggctggaaac aggcgggcat caaagaaatg cctgacgcaa gctattgcga
     9961 acaaacccgt atgttcggca aaaacatcgt caacgtcgga cacgaaagcg cggacaccgc
    10021 catcatcacc aaaccggtcg gacaaaactt ggaaatcgtc ccgctggaca atcccgccaa
    10081 cattcacgta ggcgaacgct tcaaagtccg cgttctgttc cgtggcgaac cgctgcccaa
    10141 tgccaccgtt accgccacct ttgacggctt cgacaccagc gaccgcagca aaacgcacaa
    10201 aaccgaagca caggctttct ccgacagcac agacgacaaa ggcgaagtgg acatcatccc
    10261 cttgcgccaa ggcttctgga aagccaatgt cgaacacaaa gccgacttcc ccgatcaaag
    10321 cgtgtgccaa aaacaggcga actactcgac tttaaccttc caaatcggcc attcgcacca
    10381 ttaatcccgc ccgcacaaaa atgccgtctg aaggcttcag acggcatttt ttgttcaaac
    10441 atcaatacca accgcgcagt ttcatcgctt tttcaacacg gcggatactc atcatataag
    10501 acgcggttcg caaatcgaca tcgtattctt gagccaagtt ccaaatatcg cggaacgctc
    10561 ggcgcaggac gacggtttct ttctcttgaa cttcgtcaaa ctcccaataa tagccttgca
    10621 ggttttgcac ccactcgaaa taggaaacga ccacgccgcc gcagttcgcc agaatatcag
    10681 gcacgaccaa tacgccgttt tgacgcagga taacgtctgc ctctggagta gtcgggccat
    10741 ttgcgccttc gaccacgatt gtcgcgcgga ctttaccggc attttcagaa gtcagttggt
    10801 tttccagtgc gcaaggggca agtacgtcca cttccagagc caaaagttcg gcattgctga
    10861 tttctttacc gtagccggct tcattggtga tgaaaccttt ggtttggaat tctttgatga
    10921 ttgcttccat atccaagcca ttttcattgt agatggcaac gtcaacagta gaaacggcaa
    10981 cgattttcgc accggattga tgcgcgtaat aacctgtgtg gtagccgaca ttaccgaaac
    11041 cttgaatggc gtaagtggca cccttcacgt ctttgcccag tttttccaaa gcttggacgg
    11101 cagcgagatt cacgccgtaa ccggtggctt cggtacgcgc caaagagccg ccgaactcaa
    11161 ccggtttgcc ggtaaatacg cccggtgcag aatgtttcac cacgttttca taggcatcaa
    11221 ccatccaaga catgattttg ccgttggtgt tcacatcagg tgcgggaata tcgattttct
    11281 cgccaatcag cggagaaatg gcttcggaat aagcgcgggc gatgcgttcc agttccgcct
    11341 cggaataatc gcgcggatcc aaggtaacgc cgcctttgcc gccgccataa ggaatacccg
    11401 caacgcagca tttgatggtc atccaaatcg acagggcttt gacttcgtcc agattcacac
    11461 caggatgaaa gcgcacgccg cctttatagg ggccgacggc gttgttgtgt tgcgaacggt
    11521 agcccgtgaa ggttttgacc gtgtcgtcgt cgagtttgac ggggaaattg acttccaaca
    11581 cgcgggtcgg actcttcagg atttcataaa cggccggatc ggttttcagc cggtcgcagg
    11641 cggttttgac ctgtttgcgc gcgatttcaa acggattgag ggtttctttt gcaagggctt
    11701 cagacatttt gcttcctttt cacaaaaaga ttttcagggg cgtgcatttc gctcattact
    11761 ataaccaatt tcccgacaaa atgtaatagc gtgtagtcgg cataagcccg atttgattaa
    11821 tctatatatg attttatttt tcaagccgca cggaatcagc ccggactccg cctgaaaaag
    11881 cagcgcgcgc atccgaaaaa taaatgtcaa attaaataaa gatataggca tccttttatt
    11941 ttttaaaaat ttaattggaa catcgccggg atttcaaacc cttgcccgat tccgccccga
    12001 aatccgaaaa caccgcctgc aaaacctgtt gcgattgtta acaatctata cattagaagc
    12061 cctgtgcaaa cgatgttaaa ataaaccctt tcaacccgac aggaaatcag attatgaatg
    12121 cagccatcga acacgtccaa gccgtcgcct tcgatttgga cggcacactg tgcgattccg
    12181 tccccgacct tgccgccgcc gcagaagcga tgttggaaca actcggtatg aaaccgctgc
    12241 ccgccaaagt ggtcgaaagc tatgtgggcg acggcatcgg caaactggtt caccgcgtcc
    12301 tctccaacga ccgcgaccgc gaagccgatt ccgaactgtg ggaaaaaggt ttcgtatcct
    12361 atatgaaata ctaccgcgac catttgagcg tcttcacccg cccctatccc gaaaccgaag
    12421 ccgggctggc attgctcaaa tctttgggca tcccgctggc agtcattacc aataaaaacg
    12481 aaatccttgc cgccgaactg ttgaaacagc tcggacttgc cgactacttc agcctgatac
    12541 tcggcggcga cagcctgccc gaaaaaaaac ccagccccct gccgctgcgg cacgccgccg
    12601 aagttttggg tatcgatgtt gcaaacatgg ttatggtcgg cgactcgcgc aacgacatca
    12661 tcgccgccaa agccgccggc tgcctgagcg tcggcgttac cttcggttac ggcgatatga
    12721 cgctgctctc gcaagacgat gcgacccgcc ccgactggat tatcggctcg ctgcccgaaa
    12781 tttacgaaaa cctgcaaccg cagaaaaata aagaagagta ggcattcgga cggcacgcta
    12841 tgccgtctga aacctgccgc acgccgaaac tgccgccatg aaaccgcaaa aatccctacg
    12901 cgcccgcgcg atggacatcc tctcgcgcca agaagtcagc cgcatcggtc tgaaacgcaa
    12961 acttgcaccg cacgccgaaa gcgaagaaga gttggaaaac gtgttaaacg aatttgccga
    13021 acgcaactgg cagtcggatt tgcgctatgc cgaagcctat atccgcagca aaagccgcaa
    13081 acacggttca ttgaggctga aacaggcttt ggcgcaacag ggcatagatg aagaaaccag
    13141 ccgcaacctg cttcccgacc gctcaagcga aaaacaggcc gccatagacg tgttgcgtaa
    13201 aaaattcaaa caccccgccg ccgaccttaa agaaaaacaa aaacaggcgc gtttcctcgc
    13261 ctatcgcgga ttcgatgccg ataccgttca gacggcattg aaacacgctt gggacgaaaa
    13321 ttgggaagac ggctgctgac cttccttctt gaatcttttt gcacggcaac ataaccttac
    13381 gcccgtttcc aacttttccg attgagaaca aaatgtccga acaacccgaa aaacaccaca
    13441 cctcccccat cgaagacgaa cgcaaaaacc cggtttaccg tatgggacgg gctgttgccg
    13501 gattcatgct cgtcgtttgg gcgggcgtat tggcactcgt gtttttcctg gtcttccgtt
    13561 tttggctttc ctaaacaaaa tgccgtctga aaccttcaga cggcatcggc agcccatttc
    13621 ggcaggctat cccatcatag ctttttttag cttgaattcc actttcccat tccctaaaat
    13681 ttttccacac ccatttcaaa ataccctttc ttaaaacagg tacactatga cacaacaacg
    13741 ccaactgcct tcccacgaac tcattatgtc cgaactgatg atgccggaca ccgccaattt
    13801 cagcggcaac gtacacggcg gcgaactcct gctcctgctc gaccaggttg cctattcctg
    13861 cgccagccgt tacagcggca attattgcgt taccctgtcg gttgacaaag tcctgtttaa
    13921 agaacccatc cacatcggcg atttggttac cttttacgcc gctgtcaact acacgggacg
    13981 cacctcgatg gaaatcggca tccgtgtcga agcacaaaac atccgcaccg gcgaaatccg
    14041 ccacaccaac agctgctact tcacgatggt tgcggtcaaa gacggcaaac ccgtccctgt
    14101 ccctccgctg gaaatcctga ccgaccgcca acgctgccgc tacgaaaaag ccaaaaaacg
    14161 cagagacatc agcctgcaag cctctgaaga catgtcctgc ggctgctgac ggcggactat
    14221 gccgtctgaa agacaggcac atcgcgccat ccgtttctac tgcaaacgga tgaaatcaag
    14281 caaataccca aacacagtca aacaaattta tatgccccgt cccttccgaa tgatttgaaa
    14341 acacagccgc caaaaacaaa aatgccgtct gaaaaccttt cagacggcat ttccaacttg
    14401 atttcaggca gaaagtcaga acgcgatata gctgttcggg ttaaccggtt tgccgttttg
    14461 ccgcacctcg aaatgaagct gcgttctgga agcgtcggta ttgcccatca aagcgacctg
    14521 ctgcccgcgt ttgacctgct ggccttcgcc gaccagcaat ttttggttgt gcccgtatgc
    14581 ggtcaggaag gaagaattat gctggatgat gaccagattg ccgtatcccc tcaaaccgga
    14641 acctgcataa accactttgc cgtcagccgc cgccaaaacg ggctgtcccg cattaccggc
    14701 aatatcgaca cccttgttgt tgccgccgaa atcggcaacc actttacctt gcgtcggacg
    14761 ctgccaaaca atgccgccga ccgaacgcgt tccggaaggc gaagcggcag gagattgcgg
    14821 ggcgggcgcg ggaaccgctt tattttccgc agcgggcgcg gcaggttgcg gcgcggactg
    14881 cacaggcggt tgcgcggcgg gtttcacagg ggtttgcacg gcagccggta cggcgggcct
    14941 gctttctacg gctgcggttt tcggtgcggc atatcctgcc ggtttgactt taacaatctg
    15001 accgatgctc aacatattgt cggtcatgcc gttccacgca cggaaatcgt cttgagagat
    15061 atggtagcgt ttggaaatgt tgtacaccgt gtcgccgcgc acaatagtat gcgtcgccgc
    15121 gttaatgtcg acgggtgcgg actgtacggg cggttgcgcg gcagccggta cggcgggcct
    15181 gctttttacg gctgcggttt tcggtgcggc atatcctgcc ggtttgactt taacaatctg
    15241 accgatgctc aacgtattgt cggtcatgcc gttccacgca cgaaaatcgt cttgagagat
    15301 atggtagcgt ttggaaatgt tgtacaccgt gtcgccgcgc acaatagtat gcgtcgccgc
    15361 gttgatgtcg acgggtgcgt aagaaggaac gtatgtaccc gaaacggcag gtgcagacgg
    15421 cggaacataa gcaggaggcg tataaaccgg cgcgctttgc accggcggca cataaggcgc
    15481 atcgccggca ggagccgggc tgtacggcgt tgctccatag gggttgttgt aaactgccga
    15541 agacggcgcg tcctgcatac ctgaattgcc tgcaatgacg ggggcaggct gctgggtagc
    15601 acaaccgccc aacagtgcgg caaaggaggc gcaagccgcc aaaagtgtcg tttgtttcaa
    15661 cataagataa ccttcatgtt ccgatatata gcctgaatgc ggtatatcat aataaaaatg
    15721 cgcgttcttc tcaagcgcaa agcc
//
LOCUS       DAXHTR010000036        15428 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.036, whole genome shotgun sequence.
ACCESSION   DAXHTR010000036 DAXHTR010000000
VERSION     DAXHTR010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15428
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.036"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(1..1158)
                     /locus_tag="ACFNML_000776"
     CDS             complement(1..1158)
                     /locus_tag="ACFNML_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HFC6392188.1"
                     /translation="MKFATKAIHSSYDCDEHNRALMPPIYQNSMFTLHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFAFSSGMAGIDAVWRTFLQPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKATGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAIAGPMDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIKVGLLRFSIGIEDADDIWNDISAALDTTL"
     gene            1350..2123
                     /gene="folE2"
                     /locus_tag="ACFNML_000777"
     CDS             1350..2123
                     /gene="folE2"
                     /locus_tag="ACFNML_000777"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HFC6392189.1"
                     /translation="MNTIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGIQSTVAR
                     LTMTVYLPAEQKGTHMSRFVALMEQHSEALDFAQLHRLTAEMVALLDSRAGKISVSFP
                     FFRKKTAPVSGIRSLLDYDVCLTGEMKDGAYGYSMKVMIPVTSLCPCSKEISQYGAHN
                     QRSHVTVSLTADAEVGIEEVIDYVEAQASCQLYGLLKRPDEKYVTEKAYENPKFVEDM
                     VRDVATSLIADKRIKSFVVESENFESIHNHSAYAYIAYP"
     gene            2323..2988
                     /locus_tag="ACFNML_000778"
     CDS             2323..2988
                     /locus_tag="ACFNML_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HFC6392190.1"
                     /translation="MTVLDREQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFVVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARFDSPFMLES
                     LKRRGVTEPDAMAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALGNMMTGAAMA
                     GIDSCPVEGFNYADMERVLSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(3043..3801)
                     /locus_tag="ACFNML_000779"
     CDS             complement(3043..3801)
                     /locus_tag="ACFNML_000779"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6392191.1"
                     /translation="MNPKTPPDHTEDAVRLSKRMAQLGLCSRREADGYIEQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITSE
                     NRWESDTSRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAQVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(3826..4716)
                     /locus_tag="ACFNML_000780"
     CDS             complement(3826..4716)
                     /locus_tag="ACFNML_000780"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFC6392192.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            complement(4736..5302)
                     /locus_tag="ACFNML_000781"
     CDS             complement(4736..5302)
                     /locus_tag="ACFNML_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032934 - sterol binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFC6392193.1"
                     /translation="MSALLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGGFSRESESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            complement(5362..6129)
                     /locus_tag="ACFNML_000782"
     CDS             complement(5362..6129)
                     /locus_tag="ACFNML_000782"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6392194.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTFDLGSTDAFQTALWRHHADKPVWFFLLPPSSLTHYADTVKQWAELARACN
                     VQHLIFTSSTSVYGDTARECDETATPDPQTESARQILAAEQHLLDSGISNIDILRLGG
                     LYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPGGRRLKNIIEPRHP
                     TRREFYTEEAAKLGFPAPDFAPDDSMGKIIRTVCDNS"
     gene            complement(6184..6834)
                     /locus_tag="ACFNML_000783"
     CDS             complement(6184..6834)
                     /locus_tag="ACFNML_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFC6392195.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLAYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            complement(6846..7886)
                     /gene="murB"
                     /locus_tag="ACFNML_000784"
     CDS             complement(6846..7886)
                     /gene="murB"
                     /locus_tag="ACFNML_000784"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFC6392196.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADGLRDIVRLPEFN
                     RDTVLWLGGGSNILLMEDYAGLVVHMENKGIREIARSDGMVLIEAQAGEIWHDFVLHA
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAEKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHDKQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELY
                     AEPNWLPASFSL"
     gene            complement(8065..9444)
                     /gene="norM"
                     /locus_tag="ACFNML_000785"
     CDS             complement(8065..9444)
                     /gene="norM"
                     /locus_tag="ACFNML_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HFC6392197.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            9779..10930
                     /locus_tag="ACFNML_000786"
     CDS             9779..10930
                     /locus_tag="ACFNML_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HFC6392198.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQSDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTETVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPTIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            11034..12332
                     /locus_tag="ACFNML_000787"
     CDS             11034..12332
                     /locus_tag="ACFNML_000787"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFC6392199.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDVVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVEACKPIYETMPGWRESTFGVKDYGA
                     LPENAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            12801..13184
                     /locus_tag="ACFNML_000788"
     CDS             12801..13184
                     /locus_tag="ACFNML_000788"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6392200.1"
                     /translation="MPKSLSLQDVQLRFSSKFPDKTVLKFTKNYEPVTIQCPLHGSVV
                     YGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGEIYEFASTLAASKFIGCSN
                     STLAVRLSGRTPFDRLIRYRYRLVK"
     gene            13198..13608
                     /locus_tag="ACFNML_000789"
     CDS             13198..13608
                     /locus_tag="ACFNML_000789"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6392201.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            13725..14117
                     /locus_tag="ACFNML_000790"
     CDS             13725..14117
                     /locus_tag="ACFNML_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6392202.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            14337..14717
                     /locus_tag="ACFNML_000791"
     CDS             14337..14717
                     /locus_tag="ACFNML_000791"
                     /inference="COORDINATES: protein motif:HMM:NF017109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6392203.1"
                     /translation="MTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQLLYY
                     DTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALNTPYG
                     SIRTKLDGRSNPDNLVCNRYKVLL"
     gene            14831..15391
                     /locus_tag="ACFNML_000792"
     CDS             14831..15391
                     /locus_tag="ACFNML_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392204.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
BASE COUNT         3606 a         3853 c         4309 g         3660 t
ORIGIN      
        1 ttacaaagtc gtatcgagtg cggcggaaat atcgttccaa atatcgtccg cgtcttcgat
       61 gccgatggag aaacgcagca ggccgacttt gatgcccatt tccattttca cattatgcgg
      121 cacgccgctg tgggactggg aatagcaatg gttgaccaaa ctttccacgc cgccgaggct
      181 ggaagccatt ttgaccagtt tcatgttttt aatcacgctg tttgccgctt cgcgcgtgtc
      241 gtttttgaga taaaccgtca ccacgccgcc gatgccttta ggcatttgcg cttgggcgag
      301 ttcataatgt tcgtgcgacg gcaggccggg atggaacact ttttcaatgg caggatggtc
      361 ttccagacgg cgcgcgattt cgagtgcgtt tttgcagtgt gcctccatac gcagggcaag
      421 cgttttgatg ccgcgcaaca ccagccagca gtccattggg cctgcaatcg caccggtatg
      481 caccatcata tcgtgcaaag gctgagccag ctctttggtt ttggcaacga cgatgcccat
      541 caatacgtcg gaatggccgc acaaatattt ggtagcggaa tggaacacaa aatcgcaacc
      601 catatccaac ggctgttgca gatacggcgt ggcaaaggtg ttgtcgatgc cgaccagcgc
      661 gccggtcgct ttggcttttg cggcaagggc tttgatgtct accaagcgta aaagcggatt
      721 ggacggcgtt tccagccaaa ccagtttgac cttgtgcgct ttgagcaatt cgtccaaatt
      781 gtcgggattg cctaaatcgg caaaaacaac atttaccccc catttttgat aaacatcgac
      841 caataagtca tacgcgccgc cgtaaatatc ggcgaccgcg acgatggtat cgcccggttg
      901 caggaaggta cgccatacgg catcaatccc cgccataccg ctggaaaacg caaagcctgc
      961 cgcaccgtgt tccaaatcgg caacggtgtc ttccaaaatc tgacgggtcg ggttgctcag
     1021 gcgcgaataa cggtaaggca cattttcgcc aatctcgtgc aacgtaaaca tactgttttg
     1081 ataaatcggc ggcatcagcg cgcggttgtg ttcgtcgcaa tcgtagctgg aatgaatggc
     1141 tttcgtggcg aatttcattt ggtctctgcc tatgtagatg tgaataatcg aagattttat
     1201 cactatctga aaaatagaaa caatcaatgc agctgctaaa agcgagtgat ataatctcgc
     1261 atttgcagat tgacggtata ttccccggcg gaaacgccat accatgcaca catctcaaca
     1321 attacatgaa tattaaggaa aaacaactca tgaacactat tgcagacgtg caatccagcc
     1381 gagatttgcg caatctgccg atcaatcagg tcggtatcaa agacctgcgc tttccgatta
     1441 ccctgcaaac tgcagaaggc atccagtcca ccgttgcccg tctgaccatg acggtttacc
     1501 tgcctgctga gcagaagggg acgcatatgt cgcgttttgt cgcattgatg gagcaacatt
     1561 ctgaagcctt ggattttgca caattgcaca ggctgactgc cgaaatggtc gcgcttttag
     1621 attcccgcgc cggcaaaatc agcgtttctt ttccgttttt ccgcaagaaa accgcgccgg
     1681 tttccggtat tcggtcctta ctggattatg atgtctgtct cacgggcgaa atgaaagacg
     1741 gggcatacgg ctacagtatg aaggtcatga ttcccgtaac ctcgctttgc ccgtgttcca
     1801 aagaaatttc ccaatatggc gcgcacaacc agcgttcgca tgttaccgtc agcctgactg
     1861 ccgatgcaga agtcggtatc gaggaagtca ttgattatgt ggaggcgcag gcaagctgcc
     1921 aactctacgg tttgctcaaa cgccccgatg aaaaatatgt taccgaaaaa gcctacgaaa
     1981 acccgaaatt cgtggaagat atggtgcgtg atgtcgctac ttcgctgatc gccgacaaac
     2041 gcatcaagag tttcgtcgtc gagagtgaga atttcgagtc tatccacaac cattcggctt
     2101 atgcctatat cgcctacccg taggcgcgtt tgcaatgatg cccaaatgcc gtctgaaagg
     2161 cattggggcg ttattcccga gtctgccgcc gctttgtttt cccagccgta gagagatggg
     2221 taaagtccgg atgaagcttg atggcgagga cggattgtat ggtgcggcag tgccgtatcg
     2281 gtttgaaatc aatttgcaat accagtaata aaaggatgca ctatgacggt attagacaga
     2341 gagcaggttc tatccgcatt taaaaaccgt aaatcatgcc ggcattacga tgcggcacgc
     2401 aaaatcagtg ccgaggattt tcagtttatt ttagaactcg ggcgtttgtc gcccagttcg
     2461 gtcggttcgg agccttggca gtttgttgtg gttcaaaacc ccgaaatccg acaggcaatc
     2521 aagccgtttt cttggggtat ggcggatgct ttggataccg ccagtcattt ggtggtgttt
     2581 ttggcaaaga aaaatgcccg cttcgacagc ccgtttatgt tggaaagcct caaacggcgc
     2641 ggcgttaccg aaccggatgc catggcaaaa tctttggcga ggtatcaggc gtttcaagcc
     2701 gacgacatca agattttgga cgattcgcgc gccttgtttg actggtgttg ccgccagacc
     2761 tatatcgcgt tgggcaacat gatgacgggt gcggcgatgg caggtatcga ttcctgcccg
     2821 gtggaaggtt tcaactatgc cgatatggaa cgcgtattgt ccgggcagtt cggtttgttc
     2881 gatgcggcag aatggggcgt gtccgtcgcc gcgacattcg gttaccgcgt tcaggaaatc
     2941 gccacgaagg cgcgcaggcc cttggaagaa accgttattt gggcataagg caatgccgtc
     3001 tgaaaacgca aggattttca gacggcattt tttaatgctt atttaaaacg attcaccggg
     3061 ggcgagataa cgccatttgc cgggcggcag cctgccgagc ttgaccttac ccatgcggat
     3121 gcgtttcagc ccgacgacgc gcagtccgac cagttcgcac atacggcgga tttgccgctt
     3181 tttgccctgt ttcaacacga agcgcagttg gtcttcgttt tgccattcta cttgggcggg
     3241 acgcagtttc tcaccgtcca aactcaatcc gtgattcagt aaggcaagtc ctttttcgtc
     3301 caattttccg cgcacgcgca ccaaatattc tttttcactg ccgctgtttt cgccgataag
     3361 ctgcttggcg atacggccgt cctgcgtcaa caccagtaat ccgactgaat cgatatcgag
     3421 cctgccggcg ggggcaagac cgattttgtg tttcggatcg aaacggatgc ggctggtgtc
     3481 actttcccag cggttttcag aggtaatcag ttcggcggcg gatttatagc ctttttccgc
     3541 ttgtgcgctg acatagccga cgggtttgtt cagcaggatg gtaatgcgtg ccgcctgctg
     3601 ttcgtgggct ttcttgttta gttcgatacg gtctgccggc gaaactttct gaccgagtac
     3661 ggcggttttg ccgttgaccg ttacccaacc ctgttcgata tagccgtcgg cttcgcggcg
     3721 cgaacaaagc cccagttgcg ccatgcgttt ggacaggcgc acggcatctt ctgtatggtc
     3781 ggggggagtt tttggattca taggtgtttt ccggtaaggg tcggttcaga ccaattgttc
     3841 gccccagtgc agtttttggc gcagggtttt gaaatattgg tagtcggtcg gatgtaggat
     3901 gcgtaagggg tttcggtagc ggcggatggt gatgcggtcg aggttttgca cgtcgatatg
     3961 ggtttgaccg tcgaaatgga cgcgcgcgtc gccgccttgg gtaacgagga tttcgatttc
     4021 ggacgtgtct ggaatggcga tggggcggtt ggtcatggat tgcgggcaga tggggacgag
     4081 cgtgaaggcg tgcaatcctg cctgcatgat ggggccgccg gcggcaagcg aataggcggt
     4141 cgatccggtg ggggtggaga caatcagccc gtccgaacgc tgggtataga cgaattcccg
     4201 attgacgaag acttcaaact caatcatctg tccggcaccg ccacgggaga ggacggcatc
     4261 gttgagggcg atggcgcgtt cggcggtttt gccttcgcgg atgagtgcgg cctcaatcag
     4321 gatgcgctct tcggcaaggt atttcccttc taaaacgggc aatagcttgt ccgtcatata
     4381 ttcgcgggga atttgggtca ggaagcccaa atgcccttgg ttgatgccga taatcggaac
     4441 ggcgcgcagg gcgatttcgc gggcgacgga gagaaaggtg ccgtctccgc ctaaaacggc
     4501 gaccaggtcg cagtattgcc ccagttcggt cttgttgacg atatggcagc cgacggtgtc
     4561 ttgggtatag atgcagcctt cctttatgcc gacttcgtcg agatagacgg taaagccgtg
     4621 ctgcttcaaa aaggtaatca gcgtgtgtgc ggtgtcttgg atgtcgggcg tgttggggcg
     4681 ggttacgatg ccgatgttgt gaaaagggct gttcatgtcg gatgccgtct gaaggttagt
     4741 ctatccaaat gtcgcgttcg agccggtcga ggcgttcgtt gaggcgttcc acgccgtcgc
     4801 gcagtctgct tatttcgtcg aggcagtcgg caagggcttc gttgccgatg tttgcggact
     4861 cggattcgcg ggaaaatccg ccgatttgtt cggcgatgtt cctgccgatt tgtttgatgc
     4921 cgtgtccgat gtcggcggca cggctgccga tgtctgcctg cgtgccgaaa atccgtgcca
     4981 attcgtccga tgcgcgggaa cgcaggctgc cgagcaggga cagtaccgcg atgccgagga
     5041 tgaggtcgcc ttcgagcccg atgtcgcccg ccccgggttc gccgccttgg aggattttct
     5101 gtaccgcgct gttgcggaag gtaatttcgg tgtctgcaaa gccgtttccc gccgagagca
     5161 aaccgtcttc cgtgatgcgt cccgccagtt tcagcccggc aatgttcagg gtcagtgttt
     5221 tgcctgcaaa ggcggcaagt tccgagcggc tgtccgggct ttgcagaatc aggcggttga
     5281 tgatggggag gagggcggac atattttctg atcggggcgg agaatgcctg tttgttgctg
     5341 ttgacgctta ttttacaggc ttcagctgtt atcgcaaacg gtacggatga ttttgcccat
     5401 gctgtcgtcg ggtgcgaaat caggagccgg gaaaccgagt ttggcggctt cttccgtgta
     5461 gaattcgcgt cgtgtcgggt ggcgcggttc gataatgttt ttcagccgcc tgccgccggg
     5521 gttaaatgcc gtctgaaaca ggctttcgac ggcgatatta cggtggacga tgttgatggg
     5581 gcggttgccg cccgggatgt tttgcttttg aacaaggcgg ctgacgggat ggcgttcggc
     5641 gcaataaagc ccgcccagcc gcaggatgtc gatgttggag atgccgctgt cgagcaggtg
     5701 ttgttcggcg gcgaggattt ggcgggcgga ctcggtttgc ggatcgggtg tggcggtttc
     5761 gtcgcattcg cgcgctgtat cgccgtaaac gctggtgctg cttgtgaaaa tcaggtgttg
     5821 cacgttgcac gcccgggcaa gttctgccca ttgtttgacg gtgtcggcgt aatgcgtcag
     5881 cgaggagggc ggtagcagga agaaccagac gggtttgtcg gcgtggtgcc gccacaatgc
     5941 cgtctgaaag gcatcggtgc tgccgaggtc gaaggtgtcg agatgtatgg gcaggttgat
     6001 gtcgtccgaa gtcaggctgc gttttatggc ggcgacgcgg ctgccgtgtt ggtaaaactt
     6061 ttgtgccagc ggcaggccga ggtaacctag gcctgtgatg gagatatggg gcggggggac
     6121 tgcgcgcatt cgctgatacc gtcgggtaag tgccgtctga aggctgattc ggacgctgtg
     6181 ggtttacggg ttgccgttgc cgattttccg gtcgtatttc ttgcggtgtt cgggcaaaag
     6241 ataaggacga aggaggctta aggtgcggcg gatgccgcgt gctttggagt cttcggtcag
     6301 cttggtgcgg ccgcgcaggg agctgtcgaa gtcgaaccag tatttcgtaa ccatccacat
     6361 attgacggcg agatcgttca tagcggtttg gtcggcttgg atgatgttca gaccgttgag
     6421 ctgggtcagc aggttgacca agagcgggga gactttggct tgggtgaagg tgttgtgttc
     6481 gcccaacaat tcggcactgc gtgcaagcag ggtgtttacg tcgctgaata ggaagcggta
     6541 ttcccacatc acgtcgtaaa tgcccgccat atagttgatg gagtcttcca catcagacgg
     6601 caacacggct tcgttgaggt aggcaagcag ggcttcgctg taacgtttga acagttggac
     6661 gatgatttcg tctttgttgc ggaagtggta atagagattg cccggactga tgcccaaatg
     6721 cgccgcgata tggttggtac tgatgttgcg ctcgccttcc tcgttgaaaa gcgcaaggct
     6781 ggcgtcgatg atgcgtgtgt aagtattgat tttggcgggg cgggtcacgg gcatactcct
     6841 tggatttaca ggctgaacga agcaggcagc caattgggtt cggcgtacaa ttctacctga
     6901 aaccgagcaa atactgtaaa tttgatgtgt tgcgccaact gccggacatc gtttgccgag
     6961 gcgttgtttt tgtttaccaa aaccaatgcc tgtttgtcgt gtaccgccgc gccgccgatt
     7021 tggaagcctt tcagacgaca ttggtcgatc agccagccgg cggcgagttt gaccgaaccg
     7081 tccggctgcg gataatgcgg catatcggga tgctgctcca acagggcggc agctttttcc
     7141 gcgccgatga cgggattttt aaagaaactg ccgacattac caagcacgtt aggattagga
     7201 agtttactgt tgcggattgc acacactgca tcggaaacat ctttcgccgt cggcaccctg
     7261 cccgcgctca gttcggcaac ggcggccgcc aaatcgccgt aacccaagtt cggcacaaaa
     7321 tgcgttttta atgcaaatac gaccgaaaca atcacataac gccctttacc ttcctgcttg
     7381 aacaggcttt cgcggtaggc gaagcggcag tcggcattgg aaagggttac aaaggtttcc
     7441 gtatccaaat cgaagcagcg cacgctgtga atcacatctt tcgcctctac gccgtatgcg
     7501 ccgatgttct gcacgggcga cgcgccgacc gtacccggaa tcaggctcag gttttccaaa
     7561 ccgttcaaac ccagcgcgac ggcgtgcagg acaaaatcgt gccagatttc gcccgcctgc
     7621 gcttcaatca gaaccatgcc gtctgaacga gcaatttcgc gtatgccttt gttttccata
     7681 tgtacgacca gaccggcgta gtcctccatc aaaaggatat tgctgccgcc gcccagccat
     7741 aaaacagtat ctcggttgaa ctcgggtagc cggacgatgt cgcgcaaccc gtcggcgtgt
     7801 tcaagcgcaa taaaggctcg ggcttgggcg cgcaggccga aagtgttgta gggggtaagg
     7861 tcggttcggt atcggatggg ttgcatgggt ttgaacttta aaatgagtat tgccagtggc
     7921 gggcttcagc ccatcgtttc tatagtggat taacaaaaat caggacaagg caacgaagcc
     7981 gcagacagta caaatagtac ggcaaggcga ggtaacgccg tactggtttt tgttaatcca
     8041 ctatattttt aatcggcgga gttttcagac ggccttatgc gatctgacca tctccctact
     8101 gcacaattcc aagcaccaca ccaaggcgat ggcggcgatg gtgagcgagg caatcaatgc
     8161 cgtccagaag ccgtaaatgc ccatatcgaa acggtaggcg agcaggtagc ccggcagcag
     8221 accgcagccc caaaaggcgg cggcgtggat gaacatcggc acctttgtaa ctttgtagcc
     8281 gcgcaaggcg taggaggcga tacattgggt gaagtctgcc ggttggaaca agccggcgaa
     8341 cagtaagacg gtggcggcga tgcttaaaac cgccggatca ttgttgtaca tacttaccag
     8401 cggcgaacgg aataatacca aggaaagcac ggtaatcacg gcgagcatcc atcctaacac
     8461 cagtgacacg cccgaaatat aacgcgcccg cgaaaattcg cgccgcccaa gcgaaaagcc
     8521 gatgcgcacc gtccccgccg agccgacgct ttgcggaatc atatagagaa tccccgacaa
     8581 actgatgccg acctgctgcg ccgccacata atcctcgccg aaaggcgcaa tcaaaaacac
     8641 gataaacgaa aacgcgctgg cttccaaaaa ataagacagc ccgatgggtg cgccgatttt
     8701 ccaaatctgt ttgaacaccg cccaatccgg tttgccgaat ttcgccgtca gtccgaatgg
     8761 gcggaagaaa ttttccttgg cgatataaat ccacaatgcc agcgcgctga accaaaacac
     8821 cgccatcgtc gccagtccgc agcctgcgcc gcccaaagcg ggcataccga atttgccgta
     8881 aacgaaaata tagttcagcg gcacgttcaa cacaaacgcc gcaaagctga ccaacataat
     8941 caggcgcggg cggttcaggc tggaggcgta ggcgtgcagt gcgcggtgta ccattgccgc
     9001 cggcatcgcc aagctggtga acagcatata ctgcgccatt gtgccttcca cataatcgct
     9061 caaggtcagc cagttgcgga acggcgtaat cgccgcccac atcaagacca tgccgaacac
     9121 gcccaaaaac agcccgaacc aaatcccctg ccgtcccgtt tcgcccactt cgtcggtttt
     9181 acccgcgccg taaagctggg caatcatcgg gttcagcgcc gccataatgc ccataaaggt
     9241 aatataaacc gtggcaaacg cgctgctgcc caaagccacc gccgccaagt cttccttgcc
     9301 cgcaccgccc gccatcacgg tatcgacaaa accgatgccc acctgcgcga cctgcgccaa
     9361 cagcatgggc agggcaagag cggtcagcag gcggacttct ttcaggaaga cggaaaagga
     9421 aaagcggttg aggtcgagca gcataagtgt tcaatcaaca aaaatgccgt ctgaagcaga
     9481 aaacggcagc aggaagtgat gagaaataat ggcgcacatt atatagtaaa aaaatgccgt
     9541 gccgtcagac ggcggataca gggtataaaa gtatattcag attgtgtgta ttttatggta
     9601 aagtttggtt ttaacgactt gacggcattg agccgtcgga caggggctgt tcggattctg
     9661 aatcggaagg aagcatcgcc gttttgacag cggcgtgatg cgttgcggca aagatgccgt
     9721 ctgattgcgg atcgggcagc cttttgtatt tacatgataa aatagaaggc agattctcat
     9781 gcagacatgg cagcttccgg aacatatcgc cgacgtactg cccacgaacg cgcggcagct
     9841 tgaaagcgcg agggagcagt tgttggcact gttccgcgta cacggttatg aactggtaca
     9901 gcctccgctg atggagtacg cacattccct gctgacgcat atcgatgcgg ggctttccct
     9961 gaaaaccatt ttggtaacgg acaggctcag cggcaggcag ttgggcatac gcgccgacat
    10021 cacgccgcag gtggcgcgta tcgatgccca tcttttatcc gccaaccaag ggattaaccg
    10081 gttgtgttat gccggtccgg tgttgcacgc gcagtccgac ggtctgctga atatgcgcga
    10141 acccttgcag gcaggggcgg aaatgtacgg ttttgccgac atccgtggcg acatcgagct
    10201 gatagacctg atgctgaaaa gcatgaaaat tgccgatatg ggcaaagtgc tgctttcgct
    10261 ggggcatatc ggcatatttc gcgccttgtc cgatgcggcg catttggatg cggggcagtc
    10321 tgcaacgctg cttgccttga tgcaggataa agataccgag acggtcgaag cgcaggtcaa
    10381 ggcttggaag ctggacggta tgtgggcaaa agcattctcg ctgctgccgc gcctgtacgg
    10441 cgggcgtgaa gtgttgtccg acgcgcgcgg acggttgccg gatttgtcgg cggtcggcgg
    10501 cgcgttgggc gaattgcagg cggtgtgcga cgcattcccc gattgtgaaa tccatatcga
    10561 cttgtccgag ctgcgtgtcg acaattacca cacgggcttg ctgtatgccg cctatgccgc
    10621 cgatttccac gacgcggtcg cgcgcggcgg gcgttatgac ggattgggcg gatatttcgg
    10681 cagggcgcgc ccggcaacgg gattcagttt cgacttgcgc agctttatcg ggcgtttgcc
    10741 caccatcgaa cggcagcccg ccgtgttggt cgatgcggaa gatgccgaag cggcgcgcga
    10801 agcagtcgaa gccttgcgtg aacaagggca gtgtgtcgtg attgactacg gtatcggaca
    10861 caatgtttcg gaagagcttg caggccgtct gaaaaagacg gacggcgttt ggcaggtcgt
    10921 gaaacgctaa atacccgttc atggcggatg aaaggcaaat cgtggcgggg cgcaaagccg
    10981 caccggtttg gggatttccg caataatttt taatatcgat aggttatatg gctatggcta
    11041 aaaatgttgt agtaatcggc gcacagtggg gcgacgaggg taaaggtaaa atcgttgact
    11101 ggctggcgga agaagccggc ggcgtggtgc gcttccaagg cggccacaat gcgggccata
    11161 ccttggttgt cggcggcaaa aaaaccattt tgcgcctgat tccgagcggc atcctgcatg
    11221 aaagtttgga ctgcttcatc ggttcgggcg ttgtcgtctc ccccgaagcc ctgttgggcg
    11281 aaatcgacga gttgaacgcg gcaggcgtga aaaacgtcga aggccgtctg aaaatcgcgc
    11341 cgacctgccc gctgatcctg ccttatcaca tcgcgctcga ccaagcccgc gaagcatcgc
    11401 gcggcaaagg caaaatcggc acgaccggcc gcggcatcgg ccctgcctac gaagacaaag
    11461 tggcacgccg cgccattcgc gccgccgatt tgctgcatcc tgaaaaactg cgtgaaaaac
    11521 tggatgtcgt ccttgcttat tacaacgtcc aactgcaaca tctgcacaat gccgaaccgg
    11581 ttaaagcgga agacgtgatg gcggttatcg aaaaagtcgc gccgcgcatt gcgccgatga
    11641 ttaccgacgt gtcccgcgtg ttgaacgaga aaaacaaaaa cggcgaaaaa ctgctgtttg
    11701 aaggcgcgca aggtgcgttg ttggacatcg actacggcac ttatcccttc gttacctcgt
    11761 ccaactgcct cgccggcgcg gcttcggcag gcgcgggcgt aggccctcaa atgctggatt
    11821 atgttttggg catcgtcaaa gcctatacca cgcgcgtcgg ttcgggcccg ttcccgaccg
    11881 aattgttcga cgaagtaggc gcaggtttgg cggagcgcgg acacgaattc ggttctgtaa
    11941 ccggtcgtgc gcgccgctgc ggctggtttg atgccgccgc cctgaaacgc tccatccaaa
    12001 tcaacggcat ttccggtatg tgcattacca aactcgatgt aatggacggc gttgaaacca
    12061 tcaatatctg cgtcggctac gaactgcccg acggcggcaa aaccgacatc ctgccttgcg
    12121 gctctgatgc ggtagaagcc tgcaagccga tttacgaaac catgcccggc tggcgtgaat
    12181 ccactttcgg cgtgaaggac tacggcgcat tgcccgaaaa cgccaaagca tatttgaaac
    12241 ggattgaaga agtctgcggc gcgccggtcg ccatcgtctc caccggcccc gaccgagaag
    12301 aaacgattgt gctgcatcat ccgttcgcat aaggttttgc agtaaaattg ccgtctgaag
    12361 ccctaatccg ccgttggtca taaatagtag ggtatcaaca tttcgggcta caatggtacg
    12421 tcagccaatg ccaagacgtg ccagcctgat ttgttgatgt gtgtactatt gcataggcgg
    12481 ttaagccatg caggagatga aagtgtatat gtcgcgcaaa gcccattagc cgcaagcgag
    12541 gggcggggtg cgcgagagag agggggcagg tgctaagtca ttggttatct ccttactatc
    12601 tatctatcta actacgggtg cattatgcgc ctatgcctgt tgtttgtcaa acactataca
    12661 gttaaaatgt gtaaatattt agtaaagacg tatacccttt ttctttacat ttagctccat
    12721 cggataccag tgcgttcaga gaaaatttga aaatttctat attttggttg tataatgctt
    12781 tcattttaaa aaggtctata atgccaaaat cactctcgct acaagatgtt cagttaaggt
    12841 tttccagtaa atttcctgat aagacggttt tgaagttcac taaaaactac gaaccggtaa
    12901 caatccaatg ccctttgcat gggtcggttg tttacgggaa tctacaagct gccatgaaat
    12961 cctcaacagg atgtcctgaa tgtactcgaa atcccgaaaa gccctctcca aacgccgttg
    13021 ccattaggtt ggaggacaca gagacagggg aaatctacga gtttgcaagt acactggctg
    13081 ccagtaaatt tatcggttgc tccaacagca ctttagctgt acgtttaagc ggacgcactc
    13141 cgtttgaccg attgattagg tatcgttata ggttggtaaa gtaagttcac aaccactatg
    13201 gcacttacat ttactcaagc agtttctaag ctaacctcta aatttccaca tttgaatctt
    13261 gtggagttca atggcgttcg ttacccgacg gtaatcgtct gtcctataca cgggagggtt
    13321 acttgctcta cattcaaaag tatgttggac tctaaaagtg ggtgtccaaa atgtgcatct
    13381 tatggtgtca attcccacaa aattccagaa gatacaatag ataaattatc taaaaataca
    13441 gtattggagg atactgtaac tggcgaaaca cttacattcc cctcaagagc atctgctgca
    13501 aggtcattgg gtataaaccc agcagctatc actgaccgta taaaaggtcg ggttcacaca
    13561 gagactttac ttgcagggag gtataaggtt cacatctgca ctaaatgacg tatacacttt
    13621 ttaacagtgt atacccctcg ccactatcag gtgcttctcg aaaaatttga catttttata
    13681 aatttgctat taaatccatt tgacaatcaa ttttggagtc tcaaatggcc aaatctttca
    13741 accaagcagc ttctgaactt actgatatat tccctaatat ctctctaacc ggctttgacg
    13801 gtgtgaatta cccagtaacc gttaattgtc ctatgcacgg gaacgttcgt tattcgacat
    13861 ttaatgccct tataaagtca aagtacgggt gtcctgagtg tgctaagatg tcaaaaaccc
    13921 aaacacctcc aaatgtaggg aagcccctcc tcatcctcga cacaacgacc aacgaaacac
    13981 tcacgttccc aagcgttact gccgcaggtg ctgctttagg tgtacatttc caacaaatca
    14041 atcacaggct caagggtcga acgtcgcccg acaaccttat ttcaaacagg tacaaagtgc
    14101 ttgggtacga taggtaaggt tcacaacccc ctaaatccta cgaaccaagc tcgacggacg
    14161 ttcaaatccc gattagagtt aatttatgta aagatttagt aaagacgtat accatttttc
    14221 tttacatttt gcttgcgcag gatttcaggt aggtctcaaa aaatttgaaa atttctatat
    14281 tttggttgtg taatccattt gcacataacc tatggagaca aattatgggt aagcgaatga
    14341 ctttcgatac cgccaaatca cgctttcaag agaaatttcc acatttagaa ttgttggagt
    14401 tcagtggcat ttataaacct tccagtgtta gatgtcctac gcacggggtt gtccaacttt
    14461 tgtattacga cacagctata aagtcaaagt atgggtgtcc ggagtgcggg aaacttaaaa
    14521 tgaaggaaaa tacgcctccc caaaaccaaa aacctgtctc catcctcgac accgccacag
    14581 gcgaaacact cacgttcccc agcgtacaag ctgccgccaa agccctaaac accccctacg
    14641 gctctatacg aaccaagctc gacggacgtt caaatcccga caaccttgtc tgtaacaggt
    14701 ataaggttct gctataatca acctatggaa aatattgaag aatacgcact gctgtctccc
    14761 gaagccctgc tggaacgcct ggataccgtt ttgagtatca gaatcggcgg caagggttgg
    14821 gaatccagtt atgaccgcca actttgcaca gacgctggtc gaaatacagg acagtctgta
    14881 cagggttgtg tcaaccgtcc aatacgggga tgacaacctc aagcggttga cagcggacaa
    14941 acggaagcag tatgagttga acttcaagat ttccgagggt tctacgcgtg tagagtccga
    15001 ctttaaagag actttggttc ggttcggtag agatatgctt caagatatgc cccctaaaat
    15061 ccgttcggca acgctggtag cgttgacgac cctgcttgtc ggaggggcgt tgggttacgg
    15121 ttatttggaa tacctgaagc aggttgcttc ggaagggtat cagaccgagc gtctgtataa
    15181 tgccgtcgac cgtcttgcag aatcccaaga acggataacg tccgccatcc tgaagggtgc
    15241 tagaggtgcc gatttcgtgc aaatcggcag acgttcctac agtagggagg atatatcgga
    15301 ggcaaataga cgtgcagagc gtgtcccgta tggcgcagag ttggtttcag acggcaattt
    15361 taccgctgtt ttatctgata taggggatta acaaaaatca ggacaaggcg acgaagccgc
    15421 agacagta
//
LOCUS       DAXHTR010000037        15199 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.037, whole genome shotgun sequence.
ACCESSION   DAXHTR010000037 DAXHTR010000000
VERSION     DAXHTR010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15199
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.037"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            141..575
                     /locus_tag="ACFNML_000793"
     CDS             141..575
                     /locus_tag="ACFNML_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HFC6392205.1"
                     /translation="MNFALSVIMLTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
     gene            complement(623..1966)
                     /locus_tag="ACFNML_000794"
     CDS             complement(623..1966)
                     /locus_tag="ACFNML_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFC6392206.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLASDGIPLAGVWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(1971..2624)
                     /locus_tag="ACFNML_000795"
     CDS             complement(1971..2624)
                     /locus_tag="ACFNML_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HFC6392207.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYGDIALIIAAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            2775..3065
                     /gene="gatC"
                     /locus_tag="ACFNML_000796"
     CDS             2775..3065
                     /gene="gatC"
                     /locus_tag="ACFNML_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HFC6392208.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQNI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAVAPEVRDRLYIVPQVIEE"
     gene            3128..4573
                     /gene="gatA"
                     /locus_tag="ACFNML_000797"
     CDS             3128..4573
                     /gene="gatA"
                     /locus_tag="ACFNML_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HFC6392209.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNLEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQTGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEASTNLSRYDGVRYGHRAAQFA
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            4570..5502
                     /locus_tag="ACFNML_000798"
     CDS             4570..5502
                     /locus_tag="ACFNML_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFC6392210.1"
                     /translation="MKQNRTFTFPDFRTVYSYAPLYRLQHLKYTLRKFFGKKEIYAFE
                     QFVNASPIRQGLFLHCPQNAYPLLREFVDRRFNCKRRLDAMTADFLMAEKLFGTDILH
                     QMEDYRFHLVLAHLSDGISLWLNRNDNCVEEGAWSLSLRDEAGNRLYMATFAFVGTHL
                     LTASVQGPAGEEAKDTVRRITKQLHGLRPQQLMVTALQYFAAVLGLDGAMGIAQKHQV
                     KLRWKLKKRVKMNYDAFWQEYGASLERDGYWHLPQTPARKDLTDIESKKRSMYRKRYE
                     MLDNMVAEMKDGLKTEVRGISDGIQTEKPLRRTA"
     gene            5545..6975
                     /gene="gatB"
                     /locus_tag="ACFNML_000799"
     CDS             5545..6975
                     /gene="gatB"
                     /locus_tag="ACFNML_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HFC6392211.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHAGVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPEMR
                     SAAEAVAYAKALHSLVTWLNICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAESEATIAEIIEKHGLQQMTDTGAIEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            7067..8077
                     /locus_tag="ACFNML_000800"
     CDS             7067..8077
                     /locus_tag="ACFNML_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HFC6392212.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLAAAARQNLNLPHSCKSGVCGQC
                     KAELVSGDIQMDGHSEQALSEAEKAQGKILMCRTTAQSDISINIPGYKADALPVRTLP
                     ARIESIIFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRKNGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQNSSRAVHFYWGARHQDDLYALEEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQAKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(8206..8838)
                     /gene="pdxH"
                     /locus_tag="ACFNML_000801"
     CDS             complement(8206..8838)
                     /gene="pdxH"
                     /locus_tag="ACFNML_000801"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFC6392213.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLIEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            9069..10118
                     /locus_tag="ACFNML_000802"
     CDS             9069..10118
                     /locus_tag="ACFNML_000802"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HFC6392214.1"
                     /translation="MSKQPTSKRQWRDGVAPSAKKTAKPFKSKARPKDETDKTASQPY
                     GQKASDGIKPQNLPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGERRRKKA"
     gene            complement(10187..11728)
                     /locus_tag="ACFNML_000803"
     CDS             complement(10187..11728)
                     /locus_tag="ACFNML_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HFC6392215.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVSTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVVETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAMKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQASVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMACLLIIGLLCNLAVKSVHEKHH
                     EKDIKTAAHSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(12339..13694)
                     /gene="xseA"
                     /locus_tag="ACFNML_000804"
     CDS             complement(12339..13694)
                     /gene="xseA"
                     /locus_tag="ACFNML_000804"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFC6392216.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGRLKTVLEQRYFDASQKLDWLARQIRHPRQKLDEQRASIGKLAQTLSYSMKQNLRA
                     HTARFERQTQALQHYRPDVSVCKNNIVRLQTALPAAFSQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHITFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            13891..14712
                     /gene="nadE"
                     /locus_tag="ACFNML_000805"
     CDS             13891..14712
                     /gene="nadE"
                     /locus_tag="ACFNML_000805"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HFC6392217.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            complement(14783..>15199)
                     /locus_tag="ACFNML_000806"
     CDS             complement(14783..>15199)
                     /locus_tag="ACFNML_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6392218.1"
                     /translation="LQRYHNVHRAQFEELVPLAQKVAQVHADTFPAEIAELLAYMQNE
                     LLMHMMKEERMLFPMINQGVGRGATMPISVMMHEHEEHDRAIARLKELTDNFQAPEGA
                     CGSWTRLYVLTKEMVDDLNDHIHLENDILFARVLDS"
BASE COUNT         3822 a         4506 c         3825 g         3046 t
ORIGIN      
        1 tttgggaatc tgttttaccc caatatataa agcaccatat taaggcggag tgtcttcccc
       61 actttgaccc gaacccggaa aagacaccgc ccaagccaat cctgatgctg ccccgacagc
      121 caaccattaa ggaaatccta atgaactttg ctttatccgt cattatgttg accctcgcct
      181 ctttcctgcc cgtcccgcct gccggagccg ccgtctttac ttggaaggac ggcggcggca
      241 acagctattc ggatgtaccg aaacagcttc atcccgacca aagccaaatc ttaaacctgc
      301 ggacgcgcca aaccaaaccg gcggtcaaac ccgcccaagc cgacgcaggg aagcgcacag
      361 acggcgcggc acaggaaaac aatcccgaca ctgccgagaa aaaccggcag cttgaggaag
      421 aaaagaaaag aattgccgaa accgaacggc agaacaaaga agaaaactgc cggatttcaa
      481 aaatgaacct gaaggcggtg ggaaattcaa atgcaaaaaa caaggatgat ttgattcgga
      541 aatacaataa cgccgtaaac aaatactgcc gttaatcggc tctagcgcaa acccgatgcc
      601 gtctgaagcg gcacggggtt tgtcatttct gccagtaggt tttgacgttg acgaactcgt
      661 acagcccgaa ttcggacaat tcgcgcccgt aaccggaatc tttgactccg ccgaaaggca
      721 ggcgcaaatc gctgctggta tggcggttga taaacaccga tcccgcctgt attttttcgg
      781 caaaccgcca agcgcgttcg gtatcggcgg tataaatgca ggcaccgagc ccgaacgggg
      841 aatcattggc aagggcgatg gcatgttctt cgttttcggc gcgcaaaatc agggcggccg
      901 gcccgaatac ttcttctctc cagacgcggc aggcaggatt taccctgtct aaaaccgtcg
      961 cgggataaaa ccagcctcgc ccttgtggga tttttccgcc ggtcaggcat accgcgccgt
     1021 ttgaaacggc atcttcaacc tgcccgtgaa ccctgtcccg caaatcttcg cggtgcagcg
     1081 gtgcaagcgt agtatcggga tgtttggggt cgcccatttt caatttagcg cattcggcaa
     1141 gaaacagcgt gataaaacga tcggctgcgg cttcggttac gatgatgcgc ttggcggcgt
     1201 tacacgattg ccccgcatcg cggaaacggg aataacaggc ttctgcggcg gcacgctcca
     1261 aatcagcatc gggcatcacg ataaaggcgt tgctaccgcc gagctccaac acggttttct
     1321 taaggtttgc gcccgcgtgt gccgcaagga tgcgccccgt atgcgttgaa ccggtaaacg
     1381 ccattgcatc ggtatcttca accgccttga gcgtgcccgc ctcatccagc cacacgcctg
     1441 ccagaggaat gccgtctgaa gccaaatcga acagtgcctg actgacgcgt gccacgctgg
     1501 gcgcgggttt gacggcgcac gcgttgcccg cgcacatagc gggaacggcg aaacgcaata
     1561 cctgccagac gggatagttc caaggcatga cggcaaacac cacgcccaaa ggctcgaagc
     1621 gcacctgact caaactcgcc tgcgtcgcga tggttttgtg ggcaagcagt tcgggggcaa
     1681 ggcgggcgta atagcgtatc agttcgatag acttgccgat ttccgcacgg cattcgtgca
     1741 agcagcgtcc gacttcctca cacaccattt ccgcaaaacg ctctttctcc gcctccaaac
     1801 ggtcggcaaa tttttgcagg cgcgcggcac gttcggttac gcccagttgc gcgaacgccc
     1861 cgccgcgcat tttcaaatcc gccagccgcc gttcaaactc cgcataatct tgagctggac
     1921 ggcggtaaag cgtttcgccc gtaaatacat tgacactgtg aaacatcgaa tcaacctgcc
     1981 agttgcggga atatcgtttt cagtcccgac acaataatct ccaccgatac cgccgccagc
     2041 atcataccca taatgcggtt taaaatcgtc agccccgtcg cgcccagcag gcggctgacc
     2101 ttcccggcaa cgattaaaat ggcataacaa atcgcactga ccaccaaacc ggccgcgata
     2161 atcaacgcga tgtcgccgta tgttttagcc gccgaagcgt aaataatcac ggtcgaaata
     2221 ccgcccgggc cgatggtgat cggtatggcg atgggcacga cggcaatcgc tccggcattg
     2281 cgggcggggc gcgcctgccc cgtttccggc tgcgcgccga gattctgctt ggcgggattg
     2341 tcgttgccgt tcatcataga aatggcaatc agcaacacca aaattccgcc gccgacctga
     2401 aacgaaccga cgctgatgcc caaaaccttc agcagcgtac cgccgatcag cgcaaatacc
     2461 gcaatcacgg caaacacggc aacggcggcc gtccgcgcga ccttcctgcg ctccttcgtg
     2521 ctgtgcccgt tggtcaggtc aaggtaaagc gacaacgcgc taaacggatt gatcagcacc
     2581 aaaaaagcca caatcagctt gccgatttcc atgcccaatc ccattatttc cccctccttc
     2641 aaacccgtgc ggcaggcatc cgatgctgca aattgccgcc gcaacggatt tttccgttat
     2701 aattaaaaat tcaagcaata cgccccatca tacccgaacg acggtatctt taccatcaga
     2761 caaggatgct tttcatggca ctgacacttg ccgacgtaga caaaatcgcc cgactctccc
     2821 gactgcacct gactgcggaa gaaaaagaaa aatcgcttca agaattaaac gacattttca
     2881 ctatggtcga acagatgcag aacatcaaca cagacggcat cgaaccgatg gcgcacccgc
     2941 acgaggccgc cctgcgcctg cgcgaagacg aagtaaccga aaccgaccgc gccgccgaat
     3001 atcaggcggt cgcaccggaa gtacgcgacc gcctgtacat cgtaccgcaa gttatcgaag
     3061 aataatccga atacgcttca gacggcatca gcaataccgc ccgaagccct ttaaggatgg
     3121 aagatttatg acccaataca cattgaaaca ggcaagcgtc ctgttgcagt ccaaacagat
     3181 ttccgccgtc gaactggcaa gcgcatacct tgccgccatc gccgaaaaaa atcccgccct
     3241 caacggctat atcaccatcg accaagataa aacccttgca gaagcccgtg ccgccgacga
     3301 acgtatcgcg cagggcaacg cctccgcgct taccggcgta cccgtcgcct acaaggatat
     3361 tttctgccaa accggctggc gcagcgcgtg cgcttccaaa atgctcgaca acttcatctc
     3421 cccctacacc gccaccgtcg tccaaaacct gctcgacgaa ggtatggtaa cgctcggccg
     3481 caccaatatg gatgagttcg ctatgggttc gaccaatgaa aactcattct acggtgcagc
     3541 caaaaaccca tggaatcttg agcacgtccc cggcggttcg tcaggcggtt ccgccgccgt
     3601 cgttgccgcg cgcctcgccc ctgccgcgct cggttcggac accggcggct ctatccgcca
     3661 acccgcatcg cactgcggca ttaccggcat caaacccaca tacggcacgg tttcccgctt
     3721 cggtatggtc gcctacgcct ccagcttcga tcaaaccggc ccgatggcgc aaactgccga
     3781 agactgcgcg attctgttaa acgcgatggc aggtttcgac cccaaagact ccaccagcct
     3841 cgagcgcgaa aaagaagact acacccgcga tttgaaccaa ccgctcaaag gtttgaaaat
     3901 cggcctgccc aaagaatatt tcggcgaagg caacagcgcc gatgttctga cggcattgca
     3961 aaacaccatt gatttgctga aagcccaagg cgcggaattg attgaagttt ccctgccgca
     4021 aaccaagctg tccatccccg cctactacgt cctcgcctcc gcagaagcca gcaccaacct
     4081 ttcacgttac gacggcgtac gttacggaca ccgtgccgcc caattcgccg atttggaaga
     4141 aatgtacggc aaaacccgcg ccgaaggttt cggcagcgaa gtcaaacgcc gcatcatgat
     4201 cggcacttat gtactgtcgc acggctacta cgatgcctac tatctcaaag cccaaaaact
     4261 gcgccgcctc gttgccgatg attttcagac ggcatttgca cggtgcgacc tcatcctcgc
     4321 gccgaccgca cccactgcag ccccaaaaat cggagcggat gcttcgccgg ttgaaaccta
     4381 cttgagcgat atctacacca tcgccgtcaa cctcgccgga ctgcccgcat tgaccctgcc
     4441 cgcaggcttc agcggcggcg gactgcccgt cggcgttcag cttgtcggca actacttcgc
     4501 cgaagccaaa atcctcggtg cggcgcatca aatccaactc aacagcgatt ggcacggcaa
     4561 acgacccgaa tgaagcagaa ccgcaccttt accttccccg attttcgcac cgtttacagc
     4621 tatgcgcctt tatatcggct gcaacattta aaatacacat tgcgaaaatt tttcggaaaa
     4681 aaagaaattt acgccttcga gcagtttgtc aacgcctccc ctatccgtca ggggctgttc
     4741 ctccactgcc cgcaaaatgc ctatccgctg ctgcgcgaat ttgttgacag gcgttttaac
     4801 tgcaaacgcc gtttagatgc gatgacggca gattttctca tggcggaaaa actgttcggc
     4861 acagacatcc tgcaccaaat ggaagactac cgcttccatt tggtcttggc gcacctttca
     4921 gacggcatca gcttgtggct caaccgcaac gacaactgcg tcgaagaagg cgcgtggtct
     4981 ttatctttgc gcgacgaagc aggcaaccgg ctgtatatgg cgactttcgc ctttgtcggc
     5041 acacacctgc tgacagcctc cgtacaaggg cctgcgggtg aagaagccaa agacactgtc
     5101 cgccgcataa ccaaacaact tcacggcttg cgtccccaac aactgatggt aaccgccctg
     5161 caatatttcg ccgccgtact cggcttggac ggcgcaatgg gcattgcaca aaaacatcag
     5221 gtcaaactgc gctggaaact taaaaagcgt gtcaaaatga attacgacgc attctggcag
     5281 gaatacggcg caagtttgga acgggacggc tactggcatc tcccccaaac ccccgcccgc
     5341 aaagacctta ccgacatcga aagcaaaaaa cgctcgatgt accgcaagcg ttatgaaatg
     5401 ctggacaaca tggttgcaga gatgaaggac ggtctgaaaa cagaagtacg cggcatttca
     5461 gacggcatcc aaacggaaaa accgctccgc cggacagcct gacgcgaaga ctatcgaatt
     5521 gatattttag agaaagaagc tcttatgacc tgggaaaccg taatcggctt ggaaatccac
     5581 gtccaattga acaccaaatc caaaatcttc agcggcgcat cgaccgcatt cggcgcagaa
     5641 cccaacgcgc acgccggcgt agtggaatgt gctttgccgg gcgtactgcc ggtaatgaac
     5701 cgagaagtcg ttgaaaaagc catcaaatta ggcttggctt tggatgcgaa aatcaaccgg
     5761 aaaaacgtgt tcgaccgtaa aaactacttc tatcccgact taccaaaagg ctatcaaatc
     5821 agccagttgg acttaccgat tgtcgaacac ggcaaattgg aaatcgtagt cggcgacgat
     5881 gtgaaaacca tcaacgtaac ccgcgcccat atggaagaag acgcgggcaa gtccgtacat
     5941 gaaggcttga acggcgcgac cggtatcgat ttgaaccgcg ccggcacgcc tttattggaa
     6001 gtcgtatccg aacccgaaat gcgctccgcc gccgaagccg ttgcctacgc caaagccttg
     6061 cacagcttgg taacctggct gaacatttgc gacggcaata tggcggaagg ttcgttccgc
     6121 gtcgatgcca acgtatccgt tcgcccgaaa ggtcaagcgg aattcggcac gcgccgcgag
     6181 attaaaaacc tcaattcctt ccgtttcttg gagcaggcga ttaattacga agcagaagcg
     6241 caaatcgaga ttttggaaga cggcggcaaa gtacagcagg caacgatgct gtttgatccc
     6301 gaaaaaggcg aaacccgcgt aatgcgcctg aaagaagacg cgcaagacta ccgctacttc
     6361 cccgaccccg atttgctgcc cgtcatcatt tcagatgccc aaatgcaaaa agccaaagca
     6421 gaaatgcccg agctgccgaa agaaatggca gcgcgtttcg tggcggacta cggcgtgtcc
     6481 gaatacgacg cgcgcctgct caccgcaagc cgcgcacagg ccgcctattt tgaagaagcc
     6541 gccaaagaga gcggacaagg caagccgact gccaactgga tgaacggcga actcgccgcc
     6601 gcactgaaca aagaaggcat ggaacttgcc gacagcccga ttaccgctcc gcgcctcgcc
     6661 gcgctggtag gcaaaatcgc cgacggcaca ttaagcagca aattagcgaa aaaagccttt
     6721 gaagccatgt gggcagaatc cgaagccacc attgccgaaa tcattgaaaa acacggtttg
     6781 cagcaaatga ccgataccgg cgcgattgaa gccatggtgg acgaagtgct ggcaaacaac
     6841 gccaaagccg tggaacagtt caaatccggc aacgaaaaag ccctgaatgc gattgtggga
     6901 caagtgatga aggccagcaa aggcaaagcc aaccccgcgc aggttcaaga gctgattaaa
     6961 gccaaacttg cctgacaagc aagatgccgt ctgaaaccca aatccgacgt tcagacggca
     7021 ttttcacagt ctgaccacat taaaacagca taaaaaacaa tattccatga accacaccgt
     7081 taccctgccc gaccaaacca cctttgccgc caacgacggc gaaaccgttt tggccgctgc
     7141 cgcccgacaa aacctcaacc tgccccattc ctgcaaaagc ggtgtctgcg gacaatgcaa
     7201 agccgaactg gtcagcggcg atattcaaat ggacggacac tcggaacagg ctttatccga
     7261 agcagaaaaa gcgcaaggca agattttgat gtgccgcacc accgcgcaaa gcgatatcag
     7321 catcaacatc cccggctaca aagccgatgc cctacccgtc cgcaccctgc ccgcacgcat
     7381 cgaaagtatt attttcaaac acgatgtcgc cctcctgaaa cttgccctgc ccaaagcccc
     7441 gccgtttgcc ttctacgccg ggcaatacat tgatttactg ctgccgggca acgtcagccg
     7501 cagctactcc atcgccaatc cgcccgacca agaaggcatt ttggaactac acatccgcag
     7561 gcgcaaaaac ggtgtctgct cggaaatgat tttcggcagc gaacccaaag tcaaagaaaa
     7621 aggcatcgtc cgcgttaaag gtccgctcgg ttcgtttacc ctgcaggaag atagcggcaa
     7681 acccgtcatc ctgctggcaa ccggcacagg ctacgccccc atccgcagca tcctgctcga
     7741 ccttatccgc caaaacagca gccgcgccgt ccatttctac tggggcgcgc gtcatcaaga
     7801 tgatttgtat gccctcgaag aagcacaagg gttggcatgc cgtctgaaaa acgcctgctt
     7861 cacccccgta ttgtcccgcc ccggagaggg ctggcaggga agaaaagggc acgtacaaga
     7921 catcgcggca caagaccacc ccgacctgtc ggaatacgaa gtatttgcct gcggctctcc
     7981 ggccatgacc gaacaagcaa aaaatctgtt tgtgcaacag cataagctgc cggaaaactt
     8041 gtttttctcc gacgcattca cgccgtccgc atcataattc cccggtataa aaaagattca
     8101 ggctttccgt tcggaacaca aaaaacttcc cgtccgtatt ttccccataa aaaatgccgt
     8161 ctgaagcccg attccggttt tcagacggca tatgtttttt cctgttcaag gcgacagccg
     8221 ctcgcgtatc cagccaccat ccagcaaacg gtattggatg cggtcgtgca gcctgctcgg
     8281 cctgccctgc caaaactcaa tcaaatcggg aatcacaata tagccgcccc aatgcggcgg
     8341 acgcggcacg tgcaaaggat gtttgagtcc gaccgccgcc gcctttgcca ccaataccgc
     8401 cttgttcgga atcacctcgc tctgcgcact tgcccacgca cccaaacggc tctgatacgg
     8461 gcggctctca aaatattcgt ccgacaactt ctccgccagc ctttcaacac gcccttccac
     8521 gcgcacctga cgctccagct ccggccaaaa aaacgtcatc gccgcaaatg gatgagcatc
     8581 cagcgaacgt cccttacggc tgtgataatt cgtaaagaaa acaaaacctt cagaattaac
     8641 ttccttcagc agcaccatac ggctgttggg tctgccgcgt ccgtcgaccg ccgccacatt
     8701 aaccgccgtc ggctcgttga cctgtgcgcg taccgcctcg tccaaccacc gctcgaactg
     8761 ctcgatcgga ttgtcggcgc aatcgccttc cgacaattcc cgtttactgt aatcttcccg
     8821 aatattgtgc aaatccattt accgctcctc ttgtcatttg aaagattcta cccccgcacg
     8881 caaaccgatt tcaaccgttg cacaaacttt gcctcgcttc tccaaacctc aggacacaac
     8941 gatttcatcc gcaaaaccgc cgcataaggt acaatatcga accgtccgac cgagggcggc
     9001 attttatcaa cccgtcctgc cgcacacgcc gcagaagaac cgccttatca ggcgagttag
     9061 gaaaaatgat gtccaaacag cccaccagca aacgccaatg gcgcgacggc gtagccccgt
     9121 ctgccaagaa aaccgccaaa cctttcaaaa gcaaagcccg tcccaaagac gaaacggaca
     9181 aaaccgcatc ccaaccttac ggacaaaaag cttcagacgg catcaaacct caaaacctcc
     9241 ccaaacagcg cgccgccaaa gccaaaaaac tcgtcgtccg caatcccaac caaaaaatta
     9301 tggaacacgc gcgcgattta aaagaacgcc gcagcgacct atcgcgcatg gaacccgaac
     9361 gcctgcaaaa agtgcttgcc gcgtccggcg tcggctcgcg ccgcgaaatg gaagaatgga
     9421 tcaacaacgg ctgggtaacg gtcaacggca aaaccgcgca actgggcgac aaagtcaccc
     9481 ccgacgacca cgttaccgtc aaaggcagca tcatcaaact caaatgggcc gaccgcctgc
     9541 cgcgcatcat cctatattac aaacaagaag gcgaaatcgt ttcccgcgac gacccgcaag
     9601 gccgcgtcag catattcgac cgcctgccgc aggccgccag cagccgctgg gtcgccatcg
     9661 gacgcttgga catcaacacc agcggacttc tgattctcac cacctccggc gaactcgtcc
     9721 aacgtttcgc ccaccccagc ttcgaagtcg aacgcgaata cgccgtgcgc gtactgggcg
     9781 ggctgaccgg cgaacaaatg cgcgtcctca ccgaagaagg cgtgatgctc gaagacggct
     9841 tggcaaaagt cgaacgcatc cgcgaacaag gcggcgaagg cgcgaacaaa tggtacaacg
     9901 tcgtgattaa agaaggccgc aaccgcgaag tgcgccgcat ttttgaaagc caaggactca
     9961 ccgtcagccg cctcgtgcgc gtcggcttcg gccccatcgg actgcccaac cgcctcaaac
    10021 gcggacagtt ctacgaactc aaccccgccg aagtcgccaa catcctcaaa tgggcggaaa
    10081 tgctgctgcc gggcgaacgc cgccgcaaaa aagcctaaac ccgccaaaac acaaaaatgc
    10141 cgtctgaaac atctactgtt tcagacggca ttttattcgg gcgttttcag gagaaaaggt
    10201 cgagtgcttt gacaaagacc atcaccacgc cgtaggcgag cggtatgacg gccaatgccc
    10261 aacgccacca gaccgatatg ccgccgccgg aaactttttc gcccacaagg taagcgtcgg
    10321 atatggcggt ttcgtcatcg gggttgccgc tgtgtgcggc ggttttgatg tctttttcgt
    10381 ggtgtttttc gtgtacggat ttgacggcga ggttgcacaa caaaccgata atcagcaggc
    10441 acgccatgat gtacatggtt acgctgtatg cctgtgccgc cggtatgccg ctgtcgattt
    10501 ggctttggcg tatgtaattg accagtaccg ggccgatgac ggcggcggtt gaccaggcca
    10561 gcaggatgcg cccgtgaatc gcgccgacct gataggtgcc gaacaggtct ttcaggtagg
    10621 cgggaatggc ggcaaatccg ccgccgtaca tggaaataat cacgcaaaag ccgatgatga
    10681 acagggcttt gctgccgccc tcgccgatgg agggaacggc gaaatacagc agcgaaccga
    10741 gtacgaagaa aatggtgtag gtgtttttgc gtccgatttt gtcggaaacg ctcgaccata
    10801 aaaagcgtcc gcccatgtta aacaggctca ggaggctgac gaagcctgcc gccgcacctg
    10861 cgccgactga cgcctgcctg cctacggagg tttcggaaaa gagttcctga atcatcacgg
    10921 atgcctgacc caatacgcca atgccggcgg ttacgttcag gcacaatacc cagaacaaca
    10981 gccaaaattg cggcgttttc atggcttgtg acacgttgac atgattgctg ctgaccagct
    11041 tgttttgcgt tttcggcgcg gtatagcctt caggtttcca gccgtcggca ggtacgcgga
    11101 tggtaaacgc gccgaacatc atcagtgcga ggtaaagcag gcccaatacg gcgaaggttt
    11161 caacaacccc gactgaagcg gcgtttgaaa aggcgttcat cagtgatacg gaaagcggag
    11221 aggccagcat tgcgccgccg ccgaaaccca taatcgccaa accggtcgcc atacccggct
    11281 tgtcgggaaa ccatttcatc agtgtggaaa ccggcccgat gtagcctaag cctaagccta
    11341 cgccaccgat gacgccgttg cccaaataaa gcaggaagag gttgtgcgta ctcacgccga
    11401 atgcggatac gaagaagccc aggctgaaac agcaggcggc ggcaaatatg gctttgcgcg
    11461 gccctacccg ttccatccat gtgccgaaca gggcggccga cgcgcccagc atcgcgagtg
    11521 cgatactgaa aatccaacct acggtcgtca gcttccaatc tccggccgcc gattcggtta
    11581 tgccgataag tttggtcagc ggcgcgttga atacggaata ggcgtaaatc tgcccgatgg
    11641 caaggtgtac cgccaatgct gcgggcggta cgagccaacg gttgaaaccc ggcttggcaa
    11701 tgcttgcctc acggtctaaa aacttcataa catcctcttt ctgtcagttg aaaaataaaa
    11761 tttcatttgc tcaatggaaa cttattgaaa actataaaaa aatatcggat cggtttttta
    11821 tccgccccaa gatgccccgt ctgaaacatt tcgggtgtac ggaaaggttt ctgttttttc
    11881 cgacaaattc ctgcggcttt tcgcttccgg attcccgctt ttgcgggaat gacgaattaa
    11941 agattatctt aaggtcaagg gactggattc ccgcttttgc gggaatgacg gcggcgggtt
    12001 gctgtttttt atttataaat ttctgtaact ttggatttca gccatcttca ataaattatc
    12061 gaaacgtatc aatcgtcatt cccgcgcagg cgggaatcca tccagttcgt tcgatttcgg
    12121 ttttttaagt ttcgagtaac ttctgactcg tcattcccgc gtaggcggga atccggtttt
    12181 ttgagtttca gtcatttcta atacattccc accacttttc gcttccggat tcccgctttt
    12241 tcaggaatga cgaattaaag attatcttaa ggtcaaggga ctggactccc gcttttgcgg
    12301 gaatgacggc ggagggggag cggtttttcc gattgggttt aaatgcaatc gaacaaatcc
    12361 tgctgccctt gttctttgct tacgcgcacg tcggtttcgc cgtcggcgaa ggtaatgtgc
    12421 agcttctgcc cctgcttcaa aacatcggcg ttgcggatga cttgtccgcg tgtgtttttg
    12481 acgacggaaa agccgcgctc cagaatgtgc tgcggcgaaa cggcttcgag caatgcggct
    12541 tgggcggtca ggctttggcg gcggtgggta agcagttggc tgaaggcggc gggcaatgcc
    12601 gtctgaaggc ggacgatgtt gtttttgcaa acggaaacat caggacggta atgttgcagg
    12661 gcttgggttt ggcgttcaaa acgggcggtg tgggcacgga ggttttgctt catcgagtaa
    12721 gacagcgttt gcgccagctt gccgattgaa gcgcgctgct catcaagttt ttggcgcgga
    12781 tgacggattt gccgcgcaag ccagtcgagt ttttggctgg catcgaaata gcgttgttcc
    12841 aaaacggttt tcagacggcc ttgcgcctga gcgagacgat gtagcgattc ttggcggttg
    12901 gggctgacca gctccgccgc accggtcggc gtgggcgcgc gcacgtcggc gacaaaatcg
    12961 gcaagcgtaa aatcggtttc gtgtcccacg ccactgacga cgggaaccgt gcaggcttcg
    13021 attgcgcgca cgaccggttc ttcgttaaac gcccacaagt cttcaatgct gccgccgccg
    13081 cgacagacaa tcaacacgtc gcattcggcg cgttgcgagg cggttttaat cgcttgggca
    13141 atttgtaact cgctgcctgc gccttgaaca ggtgtcggat aaacgataac gggaatttcg
    13201 ggcgcgcggc gtttcaaggt ggtaacgaca tcgcgcaatg ccgctgctgc caggctggtg
    13261 acgatgccga tacattgcgg acgggtaggc aacggctttt tgcgttccgc cgcaaacgcg
    13321 ccttccgcct gcaactgtgc cttcaaccgc tcgtaggctt cgtaaagctg ccccaaacct
    13381 ttgagccgca cctcattcac ggtaatttga aattcgcccc gcgcctcgta aatactgatt
    13441 tttcctgata cttcgatatg gtcgccttct ttcaaaggct tcgccaaacg cgccgccgca
    13501 cctttgaaca tcgcgcaacg cacctgcgcg cggctgtctt tgagcgagaa ataataatgt
    13561 ccgctggcgg cacgggtcag gttggacact tcgcccgcaa tccacaggcc ggcaaggtgg
    13621 ttttccaaaa gacttttggc aaatgcgttc aactcggaaa cggacaacac gtcagaatga
    13681 aaaaaatcag acatagaatc aatcaaataa taaaaaatat gaatatgttt tgaagcctaa
    13741 ggcggtatta gtccgcctaa attgtcaaca atattataac acgcgccatc ttgccgcccg
    13801 ccttttcccg tatgactttt ttaagcgggg aatgggaaaa atattcatca acccgccctc
    13861 aatctattca aattacaccg ccgacaagct atgatgcgga tattttcgac aggaggaaaa
    13921 atggatacgc aggcagttat cacacatatt gtccgatggt tggacgaata cgccgcccgg
    13981 gcaaatgcaa aagggtttgt cgtgggcgtt tccggcggca tcgattccgc cgtcgtctcc
    14041 gcactcgccg cccgcaccgg ccgcaccacg ctgcttctgg atatgccgat acgccaacac
    14101 cccgaccagc ttgagcgggc aaggctgcac atccgcaatc tgcaacggca atatgccaat
    14161 gtaagcgcac aaacggtcga tctgaccgac accttccaga cctttgaaca aaccgtcggc
    14221 gttcatcaga cggcatttga caatcagccg ctttccctcg ccaacgccag aagccgcctg
    14281 cgtatgctga ccctgtacta ctacgggcag atacacggac tactggttac ggggacaggt
    14341 aacaaaattg aagatttcgg tgtaggattt tttaccaaat acggcgacgg cggcgtggac
    14401 atcagcccga ttgccgacct gaccaaaacg caggtttacc ggcttgccga agcattgggc
    14461 gtggacgagg cgattcaaaa agccccgccg accgacggcc tgtgggatac ggaacgcacc
    14521 gacgaagaac agatgggcgc aagctatccc gaactggagt gggcaatggg cgtgtacggc
    14581 acgcgcaaac ccgaagattt tgaagggcgg cagcgcgaag ttctggaaat ctatacgcgg
    14641 ctgcaccgcg cgatgcagca caaaatcaac ccgattcccg tgtgccgcat tccgcccgaa
    14701 ttgttgggct gaaacgtgga aatgccgtct gaagcggaaa aaccgtattt cagacggcat
    14761 ggaaatatcc ggctcccgtt ccttaagaat cgagtacgcg ggcaaacaga atatcgtttt
    14821 ccaaatgaat gtggtcgttc aagtcgtcca ccatttcttt ggtcaaaacg taaagccgtg
    14881 tccagcttcc gcaggctcct tcgggcgctt ggaaattatc ggtcagctct ttgagccgtg
    14941 cgatggcgcg gtcgtgttct tcatgttcgt gcatcatcac gctgatgggc atcgtcgcac
    15001 cccggccgac accctgatta atcatcggaa acagcatcct ttcctctttc atcatatgca
    15061 tcagcagttc gttctgcata taggcaagca gctcggcaat ttccgccgga aaggtgtcgg
    15121 catgaacttg ggccactttt tgcgccagcg gcaccaattc ttcaaattgt gcacggtgta
    15181 cattgtggta gcgttgcag
//
LOCUS       DAXHTR010000038        14909 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.038, whole genome shotgun sequence.
ACCESSION   DAXHTR010000038 DAXHTR010000000
VERSION     DAXHTR010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14909
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.038"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(94..3579)
                     /gene="smc"
                     /locus_tag="ACFNML_000807"
     CDS             complement(94..3579)
                     /gene="smc"
                     /locus_tag="ACFNML_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HFC6392219.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNQQQDLLDYAQWQQSLAAADKATAQHQSLQAQQDETAAQVQALNDEVRALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELQTEEKQTELAEWAMQVAEHEERLPELEEAQATLNAAFQIQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSQ
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATDYAAAPQLWQHITAPTEWQHALSVILAERLHARSVPHGFVPPAPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLNYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELDNLAPELSAAEAAFKQAE
                     AAVRSSETQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIVRELAQLAEE
                     QTVLQHASDGLSDDIAILQEAAAELEHQQQTAAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIAQLEQQTLDWQERQQELALAYETEFQNDEQHIKLDELT
                     EAVHTLDEEYIVVQEKLAQIQEQGREQYARVQTLQTKLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITILEEAIAQIDDKTKARFKETFDAVNGKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCNLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            3581..3832
                     /locus_tag="ACFNML_000808"
     CDS             3581..3832
                     /locus_tag="ACFNML_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009345706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392220.1"
                     /translation="MVGKNKKEAYFNGNPAAPPHILPAKPYRIPHPRCRLKPSDGIFN
                     APEPRFRQRPMLGAAFAVLPRRRARYNPPRHPPDLETCL"
     gene            3829..4563
                     /gene="lpxH"
                     /locus_tag="ACFNML_000809"
     CDS             3829..4563
                     /gene="lpxH"
                     /locus_tag="ACFNML_000809"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFC6392221.1"
                     /translation="MKPAYFISDLHLSEKHPELTALLLRFLRSSAAGQARAVYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDRAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLENAENAV"
     gene            4819..6405
                     /locus_tag="ACFNML_000810"
     CDS             4819..6405
                     /locus_tag="ACFNML_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFC6392222.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     DDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(6573..6776)
                     /locus_tag="ACFNML_000811"
     CDS             complement(6573..6776)
                     /locus_tag="ACFNML_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HFC6392223.1"
                     /translation="MDNLNLQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            6892..7389
                     /locus_tag="ACFNML_000812"
     CDS             6892..7389
                     /locus_tag="ACFNML_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HFC6392224.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            7471..8664
                     /locus_tag="ACFNML_000813"
     CDS             7471..8664
                     /locus_tag="ACFNML_000813"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HFC6392225.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEETAIRAHSQV
                     KTIIRTLYSNPASHGANTIALVLKNDDLKAQWIAELDEMRGRIKAMRQKFVALLKAKG
                     ASQDFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(9068..10003)
                     /gene="hemC"
                     /locus_tag="ACFNML_000814"
     CDS             complement(9068..10003)
                     /gene="hemC"
                     /locus_tag="ACFNML_000814"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFC6392226.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALAAIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLVIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGARELIGAVLNTEN"
     gene            10122..10637
                     /gene="ybeY"
                     /locus_tag="ACFNML_000815"
     CDS             10122..10637
                     /gene="ybeY"
                     /locus_tag="ACFNML_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFC6392227.1"
                     /translation="MKRTKKYPFLTLQRQRFHLNFENASSAANLPSEHDFYRWAWSAL
                     KSGYRRADISLILLDEEAARAYNRDYRGKDYATNVLSFALNEGETLPCQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GYPNPYREDGH"
     gene            10639..11463
                     /locus_tag="ACFNML_000816"
     CDS             10639..11463
                     /locus_tag="ACFNML_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFC6392228.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(11592..12971)
                     /gene="nhaC"
                     /locus_tag="ACFNML_000817"
     CDS             complement(11592..12971)
                     /gene="nhaC"
                     /locus_tag="ACFNML_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HFC6392229.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFQADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHCYSLIPFALLVVLALMRINAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDVVKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GVIPSLLEAIRTFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDRLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(13262..14485)
                     /locus_tag="ACFNML_000818"
     CDS             complement(13262..14485)
                     /locus_tag="ACFNML_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HFC6392230.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
BASE COUNT         3532 a         4207 c         3829 g         3341 t
ORIGIN      
        1 gtaccgattt aaatttaatt cactataccg tctgaaaacc tgtatcgcaa cacgtcatta
       61 aaaacggcaa gattcagccg ttctgcaacc cgttcaaacg gcttccgcca tttccaacgc
      121 ctgcttgatg tccacggcga cgacgcgcga tacgcctttt tcctgcatgg ttacgccgac
      181 cagctgctct gccatttcca tggtcaggcg gttgtgggag atgtagagga actgggtttg
      241 cgccgacatt tctttgacca ggttgcagaa acgcgaggtg ttggcgtcgt ccagcggggc
      301 atcgacctcg tccaaaaggc agaacggagc ggggttgagg ctgaacagag cgaacacgag
      361 gctcatggcg gtgagggctt tttcgccgcc ggagaggagg tggatggtgc tgtttttctt
      421 gccgggcgga cgcgccataa tggacacacc ggcggtcaaa aggtcgtcgc ctatcatttt
      481 gagagtggct tcgccgccgc cgaacagggt cgggaagaag gtttggactt tgccgttgac
      541 ggcgtcgaag gtttctttga aacgcgcttt ggttttgtcg tcgatttggg cgatggcttc
      601 ttccaaaatg gtgatggcgg cctgcacgtc ttcgctttgg ctgcggtagt agccgtcgcg
      661 ttcgcgcgct tcttcgagtt cttgcagggc ggcgaggttg accgcgccga gggcttcgat
      721 ttgttgcgag aggctgccga tgctgctgtt caatactttc ggcgattctt tcgccaacgc
      781 ttcgagcgcg tccaaatcgg cggcgcgttc ggtcaggttt tgatggtagc gtttggcgtt
      841 aatcagggct tcctgctgct gcaacagggc ggtttgggtg gcagcctgaa gctgcggcag
      901 cttggtttgc aaggtttgta cgcgggcgta ttgttccctg ccctgctcct gaatctgcgc
      961 gagtttttcc tgcacaacaa tatattcttc gtccagcgtg tgtacggctt cggtcagctc
     1021 gtcgagcttg atatgctgct cgtcgttttg gaactcggtt tcataggcga gggcaagctc
     1081 ttgctggcgt tcctgccagt ctagggtttg ctgttcgagt tgggcaattt gctgttggta
     1141 gttttgtttt tgctggttga gcttgtggac ggcgacttcg gcaagcccgt attggcggtt
     1201 ggcttccaac aaggcaagct gcgcctgttt cagacggcct tgctgctctt ggcggctgtg
     1261 tgcggcggtt tgctgctggt gttcgagttc ggcggcggct tcctgcaaaa tggcgatgtc
     1321 gtctgaaagc ccgtcggacg cgtgttgcaa cacggtctgt tcttccgcca actgcgccag
     1381 ttcgcggacg atgtgttcgc ggcggatttg cccttggttg gtgcgcgcca gaagttcggc
     1441 ggcgcgttgc tgcgcttggc tgtattggcg cgtgtgttgt tgttgctgct gcatcaggtt
     1501 tttatgctgc gtttcagacg agcgcacggc ggcttccgcc tgtttaaacg cggcttcggc
     1561 ggcggagagt tcgggggcaa ggttgtccag ttcggacgcg atgccgtcga gacgcgcttt
     1621 ttgggcaatc aggctttcct gcgcgggttt ggcatagagc aggacgctga ctttgtcgac
     1681 ctgatggcct tcgggcgtga gccagatttg gtgtgcgccc aaatcgcttt gatgcgcgag
     1741 ggcgtaattc aaatcgggcg cgcacaatac gccgtcgagc cagtggtgca atgccgtctg
     1801 aaacggcggc tgcgcttgga tttggttcag caatgcctgt acgggcaggg attttttgat
     1861 gccgccggag aggccgtctg aaagccacgc cgcctgcccc tgcggcaaag gcgcaggagg
     1921 aacgaagccg tgcggcacgc tgcgggcgtg caggcgttcg gcaagaatga cggacaaggc
     1981 gtgctgccac tcggtgggcg cggtgatgtg ttgccacaat tgcggcgcgg cggcgtaatc
     2041 ggttgcctgc cagaagtcgg cggcttcctg ctgttgcgac aggatttgcg acaacgcctg
     2101 ctgctgcgcc tgcaaggtaa tgtgttgctg cttcaggctt tggaagcggt ttgaggccgt
     2161 ctgaaacgcc tcgcgggcgg cgtgtaaggc ttcttcggcg gcgatgattt gttcttcgta
     2221 atgctcttgc tggctttgca acaaggcagc ggcctcctgc gcggcggcgg tttccgcttc
     2281 gtcgggcagg ttcagggctt ggttctcctg tttcagacgg cctttgcgct cttcgtgctt
     2341 ggcaacggtt tgttcggcat gggcaagctg ctgctgcttc aacgccagtt cgcggcggat
     2401 gcggtttgcc tcatcctgct ggatttggaa ggcggcgttg agcgtggctt gggcttcttc
     2461 caattcgggc agacgctctt cgtgttcggc aacctgcatc gcccattccg ccaattcggt
     2521 ttgcttttct tcggtctgca actcgttttc ttcaagctga acgcggattt gctgctgttc
     2581 ctgatggatg cgctgcatct gcgcctgcgc cgcctgcttg tcgcgttcga tgcgttggtg
     2641 caggttctgc tgatggcgga tttgttcttc caaacgggca atctgctcgc gcaacacgcc
     2701 gcgtttgttg ctcaattcgt gtaccgcctg ctgctgcgac tgttcggcgg tctgcaaggc
     2761 gcgtacttcg tcgtttaacg cctgaacctg cgcggcggtt tcgtcctgct gcgcctgcaa
     2821 agactgatgc tgcgcggtcg ccttgtcggc ggcggcaagc gattgctgcc attgggcgta
     2881 atcgagcaaa tcctgctgtt ggttcaactg cgcggtcagg gatttgtagc gttcggcggt
     2941 ttccgcctgt ttctccagct tttccacctg acgcgccaat tcgttctgca aatcgcccaa
     3001 acgctgcaaa tgctcgcgcg tgtctttcag acggccttcc gtctccttgc ggcgttcctt
     3061 atatttggac acacccgccg cctcctcgat ataagcgcgc aactcctccg gccgcgcttc
     3121 gatgatgcgc gaaatcatcc cctgctcgat aacggcataa ccgcgcgcgc ccacgcccgt
     3181 acccagaaac aaatcagtaa tatcgcggcg gcgcacggtc tgattgttga tgaaataagt
     3241 cgattcgccc tgccgcgtca gctgccgctt gatgctcacc tcggcatact gcccccacgc
     3301 cccctgcaaa ctatggtcgc tgttgtcaaa caccagctcc accgaagccc tcggcgcagg
     3361 acggcgcgtc gccgcaccgt taaagatcac gtcctgcata ctctcgccgc gaagctgctt
     3421 cgccgaagcc tcgcccaaca cccagcgcac cgcgtcaatc acattcgact tgccgcagcc
     3481 gttcggcccg ataaccgcaa caagctgccc cggcacatga atcgtggtcg ggtcggtaaa
     3541 agatttgaag ccggagagtt tgatatgggt caggcgcata atggtcggaa aaaataaaaa
     3601 agaagcgtat tttaacggaa atcccgccgc accaccccat atcttgccgg caaaacctta
     3661 ccgcatcccg caccctcgat gccgtctgaa gccttcagac ggcattttta acgcgcccga
     3721 accccgattc cgccaacgcc cgatgcttgg cgcggcattt gccgtattgc cccgccgccg
     3781 ggcgcgttat aatccgccac gacatccgcc cgatttggaa acctgcctat gaaacccgcc
     3841 tatttcatct ccgacctgca tttgagcgaa aagcaccccg aactgaccgc gctgttgctg
     3901 cgttttttac gttcttccgc cgccgggcag gcgcgggcgg tttacatttt gggcgatttg
     3961 tttgattttt gggtgggcga tgacgaagtt tccgagttga atacttcggt tgcgcgtgaa
     4021 atcaggaaat tgtccgacaa aggcgttgcc gtgttcttcg tcaggggcaa ccgcgacttc
     4081 ctgattggtc aggatttttg ccggcaggca ggcatgacgc tgctgccgga ttactcggtt
     4141 ttggacttgt tcggctgcaa aaccctcatc tgccacggcg acactctgtg tacggatgac
     4201 agggcatacc aacgtttccg caaaatcgtg catcggaagc ggctgcaaaa actgttcctg
     4261 atgctgcccc tgaagtggcg cacgcgcctt gccgccaaaa tcaggcgcgt cagcaaaatg
     4321 gaaaaacagg tcaagcccgc cgatattatg gatgtcaatg ccgcctttac cgcgcggcag
     4381 gttcgcgcct ttaacgcgga aaggctgata cacggacaca cccaccgcga gcatatccat
     4441 cacgaaaacg gctttacccg catcgttttg ggcgactggc ataacgacta tgcttcaatc
     4501 ctccgcgtgg acggggacgg cgcggtattc gtgccgctgg aaaatgctga aaatgccgtc
     4561 tgaagccttt cgggcggctt tttttacacg cccctttccc acactccctc cgccgcatca
     4621 tcaccatcct taacccgcat taatagccat tatcgataaa atgcttgacc gattacataa
     4681 gattacgtaa agtgtgcaaa cgcataacaa ttggtcttac caatttatct tttaaccaaa
     4741 atatcgggtg tacgggattt gaacgtcttt ccaaacctcg acgcgcctta tgatgcaaat
     4801 gcctaaaaag gattcgttat ggcacttttc ctcagcatat tccccatcgt cctgctgatt
     4861 tggctgatgg tgaaaaaaaa cagtatgccc tcctacgtcg cgctgccgat taccgcagtg
     4921 ctgatttacg ccatcaaact tttctacttc gacgatgcgg gaatgctgct caacgccacc
     4981 gccgcttccg gcctcgtcaa aacgctcacg ccgattaccg tgattttcgg cgcgattatg
     5041 ttcaaccgta tgatggaaac cacgggctgc atcgatgtca tccgcaaatg gctggcgacc
     5101 atcagcccca accccgtagc gcaactgatg attatcggct gggcttttgc ctttatgatt
     5161 gaaggcgcat ccggcttcgg cacgcctgcc gcgattgccg cgccgattct gatgagcttg
     5221 ggcttcaacc cgttgaaagt ggcgattttc actttggtga tgaactccgt ccccgtctct
     5281 ttcggtgcgg tcggtacgcc gacctggttc ggtttcgcac cgctgaacct gagtgccgaa
     5341 gacatcctcg ccatcggcag gcagaccggc gtaatgcact tcttcgcagg tttcgtcatc
     5401 cccgtcatcg gcttgggctt catcgtacct tggtctgaaa tccgcaaaaa cttgggcttc
     5461 gtcgccattg ccgtcttctc ctgcaccatt ccttatgtcg cattggcgat ggtcaacgaa
     5521 gaattcccgt cgctcgtcgc cggcgcgatc ggcctgatgg tgtccgtatt cgccgccaac
     5581 caaggctggg gcttgagcaa agaccacgcc aaagacccga atgccgaaaa agtgccgttc
     5641 gcccaagtcg ccaaagcact cgcccctttg ggtatgctga tcggcatgct ggtggttacg
     5701 cgcatcaaac agctcggcat caaaggcatt ttgaccagca aagaagaatg gttcagcttc
     5761 caactgccgt ttgatttgtc caaaatcacc gtcagcgact ccctgacgat taccttcggc
     5821 aatattttcg gacaagatgt cagcgcgtct taccaaacgc tgtacgtccc ggcttggatt
     5881 ccgtttgtgc tgaccgtttg gatttgcatc ctgctgtata aaaccaaatt caaagatgcc
     5941 tggacgattt attccgtaac tttcaatcaa accaaaaaac cgctgcttgc cctgatgggc
     6001 gcgctgatta tggttcagct gatgctggtc ggcggcgaca attcgatggt gaaaatcatc
     6061 ggtaaggaat ttgccgcaat ggcgggcgaa cactgggttt atttctcgcc gtatctgggc
     6121 gcgatcggtg cgttcttctc cggctccaac accgtgtcca acctgacctt cggaccgatt
     6181 cagcagcaaa tcgccttgga taccggcctg tccgttaccc tgattctggc gttgcagtcc
     6241 gtcggcggcg cgatgggcaa tatggtgtgc ctcaacaaca tcatcgccgt atgtaccgta
     6301 ttggatgtga aaaattccga aggtgcgatt atcaagaaaa ccgttatccc gatggcgatt
     6361 tacggcgtga ttgccgtcgt cgcggcaatg attttcttcc tctaggcgaa acgccaccat
     6421 ccatagcggc agcacgcggg gaacggaaac cgccgttccc cgtccgggcg gcgcatcggg
     6481 cagaatctcg gtgccggaac agcccgcccc gccaaacaaa tgccgtctga aaccggaaaa
     6541 ggcttcagac ggcatttttc cgccgcttgc gttcaggcgt aatgatgatg cggcatcttg
     6601 ccgtcaaggc ggtagcgcgt gccgcaatac gggcaggcaa cgctgcccga ttctccttcg
     6661 cacaaaggca aaaacactct cggatgcccg ttccactgct cgttgtcggg tcccgagcaa
     6721 tacagcggca gattttccgg caacacggaa atttcctgca gattcagatt gtccatttga
     6781 ttttcctttg cgtggttggc gtgcgcccat tttacgccat cggcaggcta aaggatattt
     6841 tcggcgcaaa gccgcaatcc gctataatcc ccacttttca gacggcatac catgactgcg
     6901 cttacccttc cggaagacat ccgccaacaa gagccatccg ccctgctcta taccctcgtt
     6961 tccgcctacc tcgaacacac cgcccaaacc ggcgacgaat ccctctcctg cctgtccgac
     7021 gaccagcaca cgctgaccgc attctgctac ctcgacagcc aagtcgaaga aggcggcttc
     7081 gtgcaactca tcgcctccgg ttacggcgaa tatattttcc gcaacccgct tgccgacagc
     7141 ctgcgccgct ggaaaatcaa agccgtgccg aaagtcttgg acaaagccaa agccctctac
     7201 gaacaacacg gcaaaaccat cgaaacgctc gccgacggag gcgcagacat cgaatccctg
     7261 cgcaaacagt tccccgactt tgaagaatgg gacggcgcat actacgaagc cgccgaacaa
     7321 gacctgcccc tgcttgcaga acacatacag tcaaactggg aaaccttcgc ccatatcggg
     7381 caggcgtgat tgcgtctgtt tccagcccgt gtaaaacagc gtaaaatcgg caaacccgaa
     7441 tgaccttccg tccccatcaa ggagcaagct atgttcttca agcacatcga agccgccccc
     7501 gccgatccga ttctcggttt gggcgaagcg ttcaaagccg aaacccgccc cgaaaaagtc
     7561 aacctcggca tcggcgttta taaagacgca tccggcgcga cacccctcgt caaagccgtc
     7621 aaagaagccg aaaaacgcct gttggaaagc gaaaccacca aaaactacct caccatcgac
     7681 ggcgttgccg actacaacgc gcaaacccaa atcctgctgt tcggcaaaga ccacgaaatc
     7741 atcgccagcc gtcgcgccaa aacagcgcaa agcctcggcg gtacgggcgc attgcgtatt
     7801 gcggccgagt ttgccaaacg ccagttgaac gcgcaaacca tctggatttc caatccgact
     7861 tggcccaacc acaacgccat cgccaaagcg gtcggtatcc aagacaaacc ttatcgttac
     7921 tatgatgccg ccaaacacgg tttggattgg gacggcatga ttgaggactt gagccaagcg
     7981 caaaaaggcg acatcgtcct gctgcacggc tgctgccaca atcctaccgg catcgaccct
     8041 acgcccgaac aatgggaaac tttggcaaaa ctttctgccg aaaaaggctg gctgccgctg
     8101 tttgactttg cctaccaagg cttcggcaac ggtttggaag aagatgccta cggcttgcgc
     8161 gtgttcttga aacacaatac agaattgctg attgccagct cttattccaa aaacttcggt
     8221 atgtacaacg agcgcgtcgg cgcgttcact ttggtggccg aagacgaaga aacagcaatc
     8281 cgcgcacaca gccaagtcaa aaccatcatc cgtaccttgt attccaaccc ggcttcacac
     8341 ggtgcgaaca ccattgcgct ggtgttgaaa aatgatgatt tgaaagccca atggattgcc
     8401 gaactcgatg aaatgcgcgg ccgcatcaaa gccatgcgcc aaaaatttgt tgcgttgctc
     8461 aaagccaaag gtgcaagcca agactttgat ttcattatcg aacaaaacgg tatgttctct
     8521 ttcagcggtc tgactcccga acaagttgac cgtttgaaaa acgagtttgc catttatgcc
     8581 gtccgctccg gccgcatcaa cgttgccggc attaccgacg acaacatcga ttatctgtgc
     8641 gaaagcatcg tgaaagtact gtaaaaagca cagccatatg aaaatgccgt ctgaacctta
     8701 accagttcag acggcatctg ttttttgtta aaaaacaata aaccataaca atttcaaaca
     8761 agctaaaaac atctataaaa tcaacccaat atgatgaact caaataaaaa atacgaatat
     8821 catcattctc aaattcaaaa tttgaacgcc acagtattat ccgaagccac aaaaaccttt
     8881 ccgccgtcat tcccgcgcag gcgggaatcc gggaacccaa tgctgcaaga attgattaga
     8941 aacaactgaa accgagcaaa ctggattccc gcctgtgcgg gaatgacgaa ataaggcgag
     9001 taaaactgat gaaacaaatt gattaaaaac aataacttat attattttat ttaaattttc
     9061 gataaaatca attttccgta ttcaatactg ctccaatcaa ttcccgcgca ccgtcgtccg
     9121 ccaatttctt agcgacagcg cgtccgagcg cgtcggcata ttcggcaggg gcttgcgcgt
     9181 ccgcccgcaa cacaaccgaa ccgtcggggt gtccgaccaa gccgcgcaag gtcagcaagc
     9241 cgttttcttc cgtgcaatat gcggccaaag gcacttggca gcttccgccc aaagcgcgtg
     9301 cgagggcgcg ttcggcggta acacaggcat tggtaacgcc gtggttcaag ggtttcaaaa
     9361 cttcatacaa atcttcgcgg tgcgcggcaa tttcgatacc caatgcgcct tgtccggcgg
     9421 caggcaggct gtcggattcc gacaaaatca tgcggatgcg tccgtccaat ttcagacgct
     9481 gcaaaccggc ggcagccaag ataattgcgt cgtattcgcc gttatcgagt ttggacaaac
     9541 gggtttgcac attgccgcgc aaaggtttaa taaccaaatg cggatagcgc gcacgcaact
     9601 gggcttcgcg gcgcaggctg gatgtgccga caaccgcgcc ttcgggcatt tcttccaaac
     9661 gcgtgtattg gttggacaca aatgcgtcaa acggattggc gcgttcgccg atggccgcaa
     9721 gcgcgaaacc ttcaggcaaa tccatcggta cgtctttaat cgaatgcacc gccaaatcgg
     9781 cgcgcccgtc atataaagcc tgttccaact ctttgacaaa caagccttta ccgccgactt
     9841 ttgacaaagt tttgtccaaa atctgatcgc cgcgcgtggt catgcccaaa atctcgactt
     9901 cgcaatcggg atacagagcc ttcagacggc cttggatatg ctttgcctgc cacatagcaa
     9961 gcaggctttc gcggctggcg atgacgagtt ttttcgggtt catgttcaat gcgttcaaag
    10021 aatggaaacg gcgtaaagtc tatcatattt tgaacgtttc ttcagacggt atgccgtggt
    10081 ctatgttata tttacaccca caaaacacga aacccgccac aatgaaacgc accaaaaaat
    10141 accctttctt aacgttgcag cggcaacgtt tccatttgaa ctttgaaaac gcctcttccg
    10201 ccgccaacct tccaagcgaa cacgattttt accgctgggc gtggtctgcc ctgaaaagcg
    10261 gataccgccg cgcagacatc agcctgattc ttctggacga agaagcagcc cgcgcctaca
    10321 accgcgacta ccggggcaaa gactacgcca ccaatgtctt gagtttcgcg ctcaacgaag
    10381 gcgaaaccct gccctgccaa gtttcggaaa aactgtacgg cgatttgatt atctgcccgc
    10441 aagtcgtttt gaaagaagcc gccgaacaag gcaaaacgcc cgaacggcat tttgcccacc
    10501 tgaccataca cggtgttttg cacctgatgg gctacgacca catcgaagac gatgaagccg
    10561 aaataatgga agccgaagaa atccgcctga tgcgggcggc aggctatccc aacccctacc
    10621 gagaggacgg acattaaaat ggacggcgca caaccgaaaa caaatttttt tgaacgcctg
    10681 attgcccgac tcgcccgcga acccgattcc gccgaagacg tattaaacct gcttcggcag
    10741 gcgcacgagc aggaagtatt tgatgcggat acgcttttaa ggttggaaaa agtcctcgat
    10801 ttttccgatt tggaagtgcg cgacgcgatg attacgcgca gccgtatgaa cgttttaaaa
    10861 gaaaacgaca gcatcgagcg catcaccgcc tacgttatcg ataccgccca ttcgcgcttc
    10921 cccgtcatcg gcgaagacaa agacgaagtt ttgggcattt tgcacgccaa agacctgctc
    10981 aaatatatgt ttaaccccga gcagttccac ctcaaatcca ttctccgccc cgccgtcttc
    11041 gtccccgaag gcaaatcgct gaccgccctt ttaaaagagt tccgcgaaca gcgcaaccat
    11101 atggcgattg tcatcgacga atacggcggc acatccggct tggtcacctt tgaagacatc
    11161 atcgagcaaa tcgtcggcga aatcgaagac gagtttgacg aagacgatag cgccgacaat
    11221 atccatgccg tttcttccga acgctggcgc atccatgcag ctaccgaaat cgaagacatc
    11281 aacaccttct tcggcacgga atacagcagc gaagaagccg ataccatcgg cggcttggtc
    11341 attcaagagt tgggacacct gcccgtacgc ggcgaaaaag tccttatcgg cggtttgcag
    11401 ttcaccgtcg cccgcgccga caaccgccgc ctgcacacgc tgatggcgac ccgcgtgaag
    11461 taagcactgc cgtttctgca cagtttagga tgacggtacg ggcgttttct gtttcaatcc
    11521 gccccatccg ccaaacataa aaatgccgtc tgaaaccgtt gcaagtttca gacggcattt
    11581 cgcttatccg cttatttttt gctcaaagtc agccccgtcc aaccgaataa cagggtcaaa
    11641 gccaaactca aatagcagaa aaaggcatac ggcagatatt cccaaaccgg cacgcccagc
    11701 gcgtggctga tgaacacgcc gcatacgctc cacggtacga gcgggttaat caccgtcccc
    11761 gcatcttcca gcgtccgcga cagattgcgg gaatgtagtc cgagcctgtc gtaaacgggt
    11821 ttaaacgttt ctccagaaag caggatgctc aaatattgtt cgccaatcag gaaattaacc
    11881 ccgaccgaag tcatggcaac gctgaacgtc gcgcgtccgg cattcgtcaa gaaggtacgg
    11941 atggcctcca acagggaagg aatcacaccg agcgcaaaca acagtccgcc caaactcatc
    12001 ccgagaatca cgatggtttg cgtgaaaaac atactttcca aaccgccgcg cgaaatcagt
    12061 ttgacaacat ctttaaacgc ttcgccttcg agtttgtagc cgccgtaaaa ccacgcaccg
    12121 agctgacgca gatcgggcgt gctgtgcaga tacgttacag caacggcaac catgacggta
    12181 aagagcatgg cgacgacggc gttgatgcgc atcaatgcca aaacgaccaa cagcgcaaac
    12241 ggaatcagcg aatagcagtg caccaatccc gtggcttcaa gctggctgcg gaaggattcg
    12301 acgctgttca aatcctgcgc ggcgacgctg ggaagaagcc acagcatcag tgccgcactg
    12361 atgagccacg cgggaatggt ggtgtacatc atatttttga tgtgttcaaa caggtcgata
    12421 ccgacaatgg acgcggaaat gcccgtcgta tcggaaagcg gggacatttt gtcgccaaaa
    12481 aatgcgcccg aaacaatcgc gcccgccgtc atcgccatat cggcctgaaa cgccgccgcc
    12541 atacccataa aggcaacgcc gacagtggcg caggtggtca ggctgctgcc gatggacacg
    12601 ccgatgacgg aacacagcgc gaaggcggaa aaataaaaat aagtcgggga aatcagtcca
    12661 aaaccgtaat acatcagcgt cggaatcgcg ccgctcatca tcagcgcgct gaccatcagc
    12721 ccgatgaaga aaaacaggta aatcgcgccc ataccctgat tcaacgcgcc tatcatgccc
    12781 tgctgcatat cgttgtattt caaaccgcgc gccaagccgt acaaaatcag cacgacgatg
    12841 gcggcaataa tggacatatg cggcagccac tccaatgaaa tgatggtata gcccatcgcg
    12901 gcaatcagag cgacgacgac ggcaagtgcc tcaccgcgcg gcatatcgag taaggatttg
    12961 aaagcgaaca tgattctttg cccgatttga tataaagtta acggattcta acacaccata
    13021 ccgaatccgc ccgacgtttt cagacggcat tttagcgttt tacaaaagcc ggacagaaac
    13081 aaaacggcaa ttttctcaat ttaaattaat ttttttgaaa tataattttt tatttttttg
    13141 tttgttgaca tataagactt ttatggattg aagtattaaa acctcggcac acacgccgca
    13201 aacaccggta cgcaaaaatg ccgtctgaaa gatgttcaga cgacatttct caagaaaaag
    13261 attatttgtc cgccctatat ccgtacaccg caaaaaacac gatatacaga taacagatga
    13321 cggaaacgac aaacgaagac atcagggtgt aagtatctgc cacccagccc tgcacgacag
    13381 gaacgaccgc accgccgaca atcgcggtac acagtacacc ggaagccgcg ttggtaaatt
    13441 ttcccaatcc tttggttgcc aaagagaaaa tcgtcgggaa cataatcgag ttgaaaaaac
    13501 cgatggcaag cagcgaccac atcgccacat ccgcattgcc gctacccgtc gccatcgcga
    13561 cggcaagcag tacgaccgca gccgatgcgt taaacgccaa ataacggttg ggcgcgaatt
    13621 tcgccatcac cgccgaaccg aggaaacgtc cgaccatcgc gccgccccaa tagaacgaca
    13681 ggtaatgcgc ggcagaagca tgatccagcc ctttcagata acccaatacg ttgaccatca
    13741 acgaaccgat agacacctcc gcgccgacat agcagaaaat gcctgccgta ccgaacacga
    13801 gatgtttgta ttgccatacg ctggttttgc cgtcgtggtt gtgcgcgctt tcctcggcgg
    13861 caattttgcg cgcgtcgggc agccggatca ttttcacgaa aacggcaagg ataatcagca
    13921 gccccgccag tcccaaatag ggaatctgta cggaagaaat ctgttccgcc ttgctgacgg
    13981 tttgggttgc gtccgccaga atcaggaacg cgccgatttg cggcgcaatg gtcgtaccca
    14041 aagcgttaaa cgcctgaacc agcgtcagtg ttgccgattc cttgccgggt ttcgccagca
    14101 gggtaacata aggattaccg gcgacctgaa gcagcgttac gccggaagcc aaaataaaca
    14161 acgcgcccaa aaataccgcg taagaatggc tacccgcagc aggataaaac agcaggcatc
    14221 cgaccgccgt cagcagaaag ccgccgataa cgccgttttt gtagccgact ttgccgacaa
    14281 aagcccccat cgggatggac atcaccgcat aggcggtaaa gaaacagaat tggatcagca
    14341 tcgcctgaac gtaagacagg tcgaaaattt ctttcaaatg agggataagg atgtcgttca
    14401 tgcaggtaat aaaacccatc atgaagaaca gcgtggtcaa gacgaccaat ggggacgtat
    14461 gattgttttg tgattgcgca gacatgattt gcctttcgtg gtgtcggaat attacccgtt
    14521 tcaagaaaat agaaattgac atttcttaac ggcaatgcaa atcatactga tatttgaaca
    14581 ttttgcaaac tttatcggat gcaggcaatg cgccgatctg aaattcaagc agcagaaatt
    14641 aatattttac agttgcttgc atagatttta atagatttta aaaaattccg gcggacttgc
    14701 aaaaaccgtt cccaataaaa tgaaaatccg ccgccgtatc cggaaaattc ggaaatgccg
    14761 catcgtggca tcaaaaccgg agcggaaaga atgtcgtatg cccgacaaaa aaggggcata
    14821 cggctgtgcc gtattttttg caccgtcctc cgcccttcgg cgggggcgtg aaaatatagt
    14881 ggattaacaa aaatcaggac aaggcgacg
//
LOCUS       DAXHTR010000039        14882 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.039, whole genome shotgun sequence.
ACCESSION   DAXHTR010000039 DAXHTR010000000
VERSION     DAXHTR010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.039"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(105..1892)
                     /gene="lpdA"
                     /locus_tag="ACFNML_000819"
     CDS             complement(105..1892)
                     /gene="lpdA"
                     /locus_tag="ACFNML_000819"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6392231.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAPAAAPAQEAPKAA
                     APAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADEGLKVAIVERYKTLGGVCLNV
                     GCIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAK
                     SRKVDVIQGDGQFLDPHHLEVSLTAGDAYEQAATTGEKKIVAFKNCIIAAGSRVTKLP
                     FIPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQ
                     GADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLV
                     AAGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHE
                     GHVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASG
                     RAIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIH
                     PHPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1969..3552)
                     /gene="aceF"
                     /locus_tag="ACFNML_000820"
     CDS             complement(1969..3552)
                     /gene="aceF"
                     /locus_tag="ACFNML_000820"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFC6392232.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAASAAAAPVAAGGATVQV
                     AVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVVKAVFLKVG
                     DKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAPSAPAAAKID
                     EAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAKPAA
                     AGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHEEAD
                     MTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKNYFN
                     IGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTISSL
                     GGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDGAAG
                     MRFTVFLAKLLKDFRRITL"
     gene            complement(3702..6365)
                     /gene="aceE"
                     /locus_tag="ACFNML_000821"
     CDS             complement(3702..6365)
                     /gene="aceE"
                     /locus_tag="ACFNML_000821"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFC6392233.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKALVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSDFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVVT
                     GAELLKADFGVEADIWSCPSFNLLHRDAVEVERFNRLHPLEAEKVPFVTSKLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALSA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7059..8771
                     /locus_tag="ACFNML_000822"
     CDS             7059..8771
                     /locus_tag="ACFNML_000822"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFC6392234.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVGFAGKVYADFATEKGADWVIGANEDGYHYTGFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTQAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVVRVTA
                     LLNG"
     gene            8834..9490
                     /locus_tag="ACFNML_000823"
     CDS             8834..9490
                     /locus_tag="ACFNML_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFC6392235.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGTGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKEKGLPWLKAKGFRHSACVSDFAAAGRIGNPENVLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVRASFTVGC
                     "
     gene            complement(9500..9955)
                     /gene="ruvX"
                     /locus_tag="ACFNML_000824"
     CDS             complement(9500..9955)
                     /gene="ruvX"
                     /locus_tag="ACFNML_000824"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFC6392236.1"
                     /translation="MHKIPKGTALAFDFGEARIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9948..10496)
                     /locus_tag="ACFNML_000825"
     CDS             complement(9948..10496)
                     /locus_tag="ACFNML_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFC6392237.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVERGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALFSGAGHA"
     gene            complement(10498..11058)
                     /locus_tag="ACFNML_000826"
     CDS             complement(10498..11058)
                     /locus_tag="ACFNML_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HFC6392238.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11386..13176
                     /locus_tag="ACFNML_000827"
     CDS             11386..13176
                     /locus_tag="ACFNML_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HFC6392239.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKSVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQARGNLASVNRKQREAWDKFQKLNTELNRLKTEVAATKAQI
                     SRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAV
                     QEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNE
                     QQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNL
                     TAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYSTA
                     PATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIG
                     SSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13303..14787
                     /locus_tag="ACFNML_000828"
     CDS             13303..14787
                     /locus_tag="ACFNML_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HFC6392240.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLINSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
BASE COUNT         3532 a         3688 c         4188 g         3474 t
ORIGIN      
        1 gtcggagaag atattttaat ccgctgtaaa atagaaaatg ccgtctgaac atttcagacg
       61 gcatttgctc aaataaacct tatcggctgt ttattcagtc ggatttattt tttcttttgc
      121 ggaggcaggt cggtacaagt acccaatgcc acttccgccg ccataccgat ggattcgccc
      181 aaggtcgggt gcgggtggat ggttttgccg atgtctgccg cgtcgcagcc catttcgatg
      241 gcaaggcaga cttcgccgat catgtcgcca ccgttcggac cgacaatgcc gccgccgatg
      301 atgcggccgg tttcggcatc aaaaatcagc ttggtaaagc cgttgtcgca accgttggca
      361 atcgcacggc cggaagccgc ccacgggaag ttggctttgg tgattttgcg gccggatgct
      421 ttggcggaca actcggtttc gcccacccac gccacttcgg gggaagtgta ggcaacgccc
      481 ggaatcacgc gcgcgtcgaa gtaggctttg tggccggcgc agttttcagc agcaacgtgg
      541 ccttcgtgaa cggctttgtg tgccaacatt ggttgaccga cgatgtcgcc gatggcgtag
      601 atgtgcggca cattggtacg catttgtttg tccacttcga tgaagccgcg atcggtaacg
      661 gcaacgcctg ctttttccgc gctgatgagt ttgccgttgg gcgcgcggcc ggcggcaacc
      721 aatacggcat cgtaacgttg cggctcttta ggcgcatttg caccttcaaa ggtaacgtaa
      781 acgccgtctt ctttcggctc gactgcaacg gttttggtgt tgaccataat gttgtcaaaa
      841 cggtattcgt tttgtttttg ccatactttc accaagtcgc ggtctgcgcc ttgcatcagg
      901 ccgtccatca tttcaaccac atccaaacgc gaacccagcg tgctgtaaac cgtacccatc
      961 tcgaggccga taatgccgcc gccgataatc agcagtttgc ccggtacttc tttcagagcc
     1021 aatgcgccgc tggaatcgat gatgcgcgga tcttcaggaa tgaaaggcag tttggttacg
     1081 cggctgcctg ctgcaatgat acagtttttg aaggcaacga tttttttctc gccggtagtg
     1141 gctgcctgtt cgtacgcgtc gccggcagtc agcgacactt ccaagtggtg cgggtctaag
     1201 aattgcccgt cgccttggat aacgtccact ttacggcttt tcgccatacc tgccaaaccg
     1261 cccgtgaggc gggaaactac gccgtctttg taggcgcgaa gcatatcgat gtcgagttcc
     1321 ggctcggggt atttgatacc gttggcagcc aagtggcgca cttcgtcgat aacggcggca
     1381 ttgtgcaaca aggctttgga agggatacag ccgacgttca ggcaaacgcc gcccaaagtt
     1441 ttgtaacgtt cgacgatggc gactttcaag ccttcatcgg cagcggcaaa tgcagcggag
     1501 taaccgccgg gaccgccacc caatacgacc acgtcgtact cggcatcggc agaaccgccg
     1561 aattgcgcgg cttgcggagc aggagcagca gctttagggg cttcttgcgc cggggcggca
     1621 gcaggtgctt cagctttggg agcggctgcc gtgccttcag cttcaacgac gacaatcaag
     1681 ccgccttcgg agattttgtc gccgactttg actttcactt ctttgaccac gcccgcaact
     1741 tcggcaggca cgtccatcgt cgctttgtcg gtttccaggg taatcagggt atcgtcaacg
     1801 gcgatggtgt ctcctacgtt cacttcaact gcgataatat ctacattttc gtgtccgcca
     1861 atgtcgggca ctttcaattc aactaagctc atgtctcatc tttctgaatg atgttcaaac
     1921 ttccttttca gacggcacag gccgctatgc cgtctgaagc cgatcgggtt acagggtaat
     1981 gcggcggaag tctttcaaca gcttcgccaa gaatacggta aagcgcatac cggccgcacc
     2041 gtcgatgacg cggtggtcga aagacaggct caacgggcac atcaggcgtg gggcaaattc
     2101 tttgccgttc caaaccggtt tgatttggga tttgcacacg cccaagatgg cgacttcggg
     2161 agcgttcaca atcggcgtga aacctgtgcc gccgataccg cccaagctgg aaatggtaaa
     2221 gcacgcgcct tgcatttctt gcggtttgag cttgccttcg cgggcttttt tagacaattc
     2281 ggtcagctct tggctgattt gtttcaagcc tttttgatcc acgtctttga tgacgggaac
     2341 aaccaagccg ttcggcgtat cggctgcgaa accgatgttg aagtagtttt tcagcaccag
     2401 gttgtcgccg tccagagaag cgttgaattc ggggaaggct ttcagcgcgg aaacggaggc
     2461 tttgatgatg aacgctagag gagacagttt cacgccttcg cgttcccatt ctttgttcag
     2521 ttgtttgcgg aattcttcca gctcggtcat atctgcttct tcgtgtacgg taacgtgggg
     2581 aatcaccacc cagttgcgag acaggttttg accggagatt ttcttaatgc gggacaattc
     2641 tttaacttcg acattgccga atttggagaa gtccacttta ggccacggca gcaagtccag
     2701 accgctgccc aaagatgcgc cggctgcggc aggttttgcc gcgccaccct gcattacgga
     2761 tttcacaaag gctttgatgt cgtcgcccac gatgcggcct ttcaagccgg tacctttgac
     2821 ttggcccaaa tccacgccca attcgcgcgc cagtttgcgt gcggaaggac cggcgtgtgc
     2881 tttggcgaaa gcggcctcgt cgattttggc agcggcaggt gcagaaggtg caggcgcggc
     2941 ggcgggtgcg gcggcagcag tcggaggcgg agcggcagcc ggtgcggcag cttgagcggc
     3001 ttgagcaggg gctgctgcgg cagagccgac ggtttctact tcgataatgg cagagccttc
     3061 ggacactttg tcgccgactt ttaagaatac ggctttaacg acaccggcag cggtacaagg
     3121 tacgtccatt gtcgctttat cggtttccaa agtaatcagc gtgtcgtctt cggcaacggt
     3181 gtcgcccact ttgatttcaa cggcgattac atccacatcg gtatggccgc cgatatcggg
     3241 aacggctact tgaacggttg caccgcctgc ggcaacgggt gcggcagcag ccgaggcggc
     3301 ttcggcagcg gcggcaccgg tttcaacggt caaaattacg ccgccttcgg agattttgtc
     3361 gccgactttg acttttactt ctttcacgac accggcagca tcggcaggca catccatcgt
     3421 ggctttgtcg gtttccagtg taatcagggt gtcgtcaacg gcgatggtgt cgcccgcttt
     3481 aacttctacg gcgatgatgt cgacgttttc gtgaccgccg atatcgggga ctttgatttc
     3541 tacgatactc atttgagttc ctttaatctt gttcagttgg atgccgtctg aaagctgctt
     3601 caggcggcat cggtacggtt tgtctgctca aaccattcgg gcattcggtt ttcagacggc
     3661 ctgatgtcgg aatggggcaa acagaccgtc tgaaacatca atcagcgttt ccagctagga
     3721 gctgaatcgg ctttgatgcc gtatttctca atggcttgtt gaacggtttc tttgctgact
     3781 ttgccttgtt ccgccaatgc gctcaatgct gccacggcaa cgttgtagcg atccacttca
     3841 aagaagcggc gcaggttggc gcggctgtcg gagcggccga agccgtcagt acccaagacg
     3901 tggtagtcgt tcgggatgta ggcgcggata cggtcagcat agctgcggat atagtcggta
     3961 gcggcaataa ccggaccgtc atgaccttgc aatttggaag taacgaaagg tactttttcg
     4021 gcttccagcg gatgcaggcg gttgaagcgt tctacctcga cagcgtcgcg gtgcagcagg
     4081 ttgaaggatg ggcaagacca aatgtctgct tccacgccga agtcggcttt cagcagctcg
     4141 gcaccggtaa ccacttcttg caaaatcgta ccggaaccca tcagttggac tttcttgtcg
     4201 cctttgccgc cggctttcag caggtacata cctttcaaga tgtcttgttc cgcaccttcg
     4261 ggcatatccg gatgggtgta gttctcgttc atcagggtga tgtagtagaa cacgtcttca
     4321 ttattggcat acatacggcg cagaccgtct tgtacgatga cggcgacttc gtattggaaa
     4381 gtcgggtcat aagatacgca gttcggaatc aggtcggcct ggatgtggct gtggccgtct
     4441 tcgtgttgca ggccttcgcc gttcagcgtc gtacggccgg cagtaccgcc cagcaggaag
     4501 ccgcgcgcgt gcatatcgcc cgccgcccaa gccaagtcgc cgatacgttg gaaaccgaac
     4561 atagaatagt aaatgtagaa cggaatcatc gcgaagtcgc tgttggcgta gctggttgca
     4621 gccgcaatcc agtcggccat cgcgcccggt tcgttaatac cttcttgcaa gatttgaccg
     4681 tcaacggatt ctttatagaa catcagttgg tctttgtctt gaggggtata ttgctgacct
     4741 ttcggattcc aaataccgta ttggcggaac ataccttcca tgccgaaagt acggctttcg
     4801 tcgggaacga taggtacgac gcgtttgccg atttttttgt ctttcagcaa agtggacagg
     4861 atgcggacga atgccatcgt ggtcgagaac tcgcgttcac cgctggattt gagttgtgcg
     4921 tcgaatgctg acagctcggg cacttccaat acttcctgcg tcggtttgcg ttgcggcagg
     4981 tagccgccca aagcatcgcg gcgtgcgtgc aggtatttgt attcttccgt atcgggggca
     5041 aaagtcaggt aaggcagatc gccgctttcg atttgttcgt cggtaaccgg aatgtcaaag
     5101 cggtcgcgga attgtttcag ggacgctttg tccatttttt tggcttggtg ggcaacgttc
     5161 tgaccttcgc cggatgcgcc cataccgtaa cctttaatgg ttttcgccaa gatgacggta
     5221 ggtttgccgt cggcatggtt cgctgcgcgg tcgtaggcgt tgtacacttt ttgcgggtcg
     5281 tggccgccgc ggttcaatgc ccagagttgc tcatcggtca tatcggcaac caatgctttc
     5341 agttcgggcg tattgaagaa gtgttcgcgc acatacgcgc cgtctttgga tttgtaagtt
     5401 tggtagtcgc cgtccaaaca ttcttccata cgttggcgca ggataccgtc tttgtctttc
     5461 gccaagaggc ggtcccagcg gcggccccaa atgactttga cgacattcca gccggcgccg
     5521 gcaaagttgc cttccaattc ttggatgatt ttgccgttgc cgcgtaccgg accgtccaag
     5581 cgttgcagat tgcagttgat gacgaaaatc aggttgtcca agccttcgcg tgcagccagt
     5641 gcgattgcac cttgagattc gggttcgtcc atttcgccgt cgccgcagaa acaccatact
     5701 ttacggcctt tggtttttgc caaaccacgc gattccaagt atttcaggaa acgcgcctga
     5761 taaatcgcca tgatgggccc caagcccatg gatacggtcg ggaactgcca aaagtcgggc
     5821 aagaggtggg ggtgcggata ggaaggcaga ccgtgtccgt ccacttcttg gcggaagttg
     5881 ttcagctggt cttcggtcag acggccctcg acgaatgcgc gtgcatagat gcccggggcg
     5941 acgtgacctt ggaagaagac caaatcgcct tcttcgcctt cgcctttggc tttccaaaag
     6001 tggttgaaac cgacttcgta catggtggcg gcagattgga aagatgcgat gtgcccaccc
     6061 agttccaaat ctttcttgcc ggcgcgcaat acgatggcgg cggcgttcca gcgcacgaat
     6121 gcgcggatgc ggtgttcgat gttttggtcg cccggaatgc ctttttcgtt ttcaaccgaa
     6181 acggtattca aatacggtgt ggtcgtgccg tgtggcatac gtacgccctt gtcgcggcag
     6241 tatttgacca ggttttccaa gaggtattgc gcgcgttcgc cgccttcata ttcgaggacg
     6301 gagcttaacg cgtccagcca ctcttgggtt tcgatagggt caacatcgtg taattgggtg
     6361 gacatagtat ctatccttta tgttgagtgt ttaatcggca gggagggtgt agccaacccc
     6421 tttattttcg tttgtgaaaa ataataagca aaaacgaaaa tttctaaatc aaaccgaaaa
     6481 aaataattta agtttaatgt tttcaaaaaa ttataaaagt tttggaaaca agggaaaact
     6541 tgaaaaaaat gttcgatctg ctccacacga aatcctatca gcaatcgggt ctgctggcaa
     6601 ataaccatgc ctgtaaaagc aggttttata ctgaagttgg cgtttggtaa ggtcaagtct
     6661 ttttgatttc gaaatattct gtttgatttt ggcgatattt tttcggaatt gtggtatatc
     6721 gaatcattat gtctctgtcg gcaattttcc tattgtagaa aatctataca caagctttgt
     6781 ggataaccgt gttgataagc cacggcttgt gttaaaaaag tcttacgcaa caaatgtacg
     6841 cttattttga ttaaaaaata ggcacattta aatctataaa aatcatgtgc ttatgttcaa
     6901 tatgatgttt ctggtcatgt ttttgccgtc tgttatggga tttctcccgc gcttggggca
     6961 gggggtgaat tttggaggtt ggtgcaaaac ctgctttttt cgcgttacaa tctgcacccg
     7021 atttactttt gaatttgcct gtttggaaaa atacttttat gaaagccagc caattcttta
     7081 tctctacttt aaaagaagcc cctgccgaag ccgcgcttgc cagccacaag ctgatgattc
     7141 gcgccggtct gattaaagcc aacgcgtccg gcctttatac ttggatgccg atggggctgc
     7201 gcgtgttacg caaagtcgaa aacgtcgtgc gcgaggaaat ggcgcgcgcg ggcagcgtgg
     7261 agctgctgat gccggtggtg cagcctgccg agctgtggca ggaatccggc cgctgggaat
     7321 tttacggtaa agaactgctg cgcctgaaag accgccacga ccgcgatttc tgcatgggcc
     7381 cgacctgcga ggaagtcatc gccgacatcg tgcgcaaaga aatcaacagc tacaaacagc
     7441 tgccgaaaaa cttttaccac atccaaacca aattccgcga cgaagtgcgc ccgcgtttcg
     7501 gcgtgatgcg cgcgcgcgag ttcgtcatga aagacgctta ttccttccat gccgactacg
     7561 cctcgcttca gacgacctat gatgccatgt atgactctta ctgccgcatc ttcacccgtc
     7621 tgggcttggc attccgcccc gtcgccgcag acaccggcag catcggcggt acgggttcgc
     7681 acgagtttca agtgttggcg gaaagcggcg aagatgttat tgcgtacagc gacacttccg
     7741 attacgccgc caatatcgag ttggcaccga ccttgccgct taaaggcgaa cgcgccgctg
     7801 ctcaggctgt gttgaccaaa gtacatacac caaacgtcaa aaccattgag tctttggttg
     7861 aattcctgaa tattccggtt gaacaaaccc tcaaatccat cgtggtcgaa ggcgaaaacg
     7921 aaggcgaaat cgtcttatta ctgttgcgcg gcgaccatga gttcaacgac atcaaggcag
     7981 aaaaactcgc aggcgtgaga tcgccattga cgatggcaag tcctgccgcg attgtcgaac
     8041 agttcggcgc aaacggcggc tcgctgggtc cggtcggctt tgcaggtaaa gtttatgccg
     8101 atttcgccac tgaaaaaggc gcggactggg tcatcggtgc gaacgaagac ggctaccact
     8161 ataccggctt caacttcggg cgcgatgccg ccgagcctga attcgtcgat ttgcgtaatg
     8221 tggtcgaagg cgacgaaagc cccgacgggc aaggccgtct gaaactggcg cgcggcattg
     8281 aagtcggaca cgtcttccag ttgcgcgaca aatacaccca agccatgaac gtaagcttcc
     8341 ttgacaacaa cggcaaatcg caaattatgg aaatgggctg ctacggcatc ggcatcaccc
     8401 gcgtcgttgc cgccgccatc gagcagaata acgacgaaaa aggcatcatc tggaccaaag
     8461 caatggcgcc gtttgaagtc gttatcgtgc cgatgaacta caaaaaatca gacaccgtac
     8521 gcgaagccgc cgacaaaatc tatgccgaat tgctggcggc aggcgcggat gtgctgctgg
     8581 atgaccgcga cgaacgcgca ggcgtattgc tgaacgattc cgagctgttg ggtattccgc
     8641 accgcatcgt catcggcgac cgcgctttga aagaaggcaa tgtcgaatac gccgaacgcc
     8701 gcaacaacga agcgcaggca gttgcaatcg gagaaattgt tgtgcgtgta acagctttat
     8761 taaatgggta agcttggtca aatgccgtct gaaaataatt tcagacggca tttttatttg
     8821 gggagaaagt gaagtggctt cagtattttt agaaggagaa gcagtcgagg tcggcaatat
     8881 tttctgtatc ggcaggaact atgccgcaca tattgaagag ttaaaaaacg aaataccttc
     8941 cgaacctgtg gtgtttatga agccgtcagg cagcattttg aacagcggcg gtacaatctt
     9001 gctgcccgag tttagtaggg atgtgcagtt tgagtgcgaa ctcgtgctgc tggtcggtaa
     9061 ggattcagac ggcacgggcg agggtaaaga tattttggga tgtgtcgcag gatatggtgt
     9121 agggctggac cttacggcac gggatatcca atgccgtctg aaggaaaaag ggctgccctg
     9181 gctgaaggca aaagggttca ggcattccgc gtgcgtgtcg gattttgcgg cggcaggcag
     9241 aatcggcaat cccgagaatg tgttgttttc attaaaacaa aacggcgtat tgaaacagcg
     9301 cggggatacc ggtttgatga tttacccgat tcgggaaatt ttacacaaac tggcggcaga
     9361 ttacggtttg gggaaagggg atttggtgtt taccggaacg ccctccggcg tgggcgcaat
     9421 cggtgcgggg gacaatttgg cattggagct ggacggcttg gtgcgcgcct ctttcacggt
     9481 agggtgttaa ccgcgccgct taaccctcac gcccgttgaa acattccgcc ggaccgccct
     9541 cgaaaaaacc gtgcaagatg gcttgcgccg ccacttggtc gagcaccgat ttgcgttttt
     9601 tgccgaagac ctgtgcttcc gaaagcaggc tttcggcata gacggacgac agccgttcgt
     9661 caacccaata gacggggaga ttgaacctgc cgttcagcct gcgtccgaac ttgcgcgaca
     9721 ggtgcgtcat ttcatgtttc gtgccgtcgg tatgcacggg cagtccgacg acaaaataac
     9781 gcggctgcca ttcttgaacc agcttggcga ttgccgcgaa cttttcatcg ttgctgccgc
     9841 cggtaacggt gctcaaagga tgggatagcc ctaattccgc gtctccttgt gccacgccga
     9901 tacgcgcttc gccgaagtcg aatgccagtg ccgttccttt tggaatttta tgcatggccg
     9961 gctcccgaaa acagggcgag cgggtcgatg ccgagtttgg cgaatgcggc ggcgtaacgg
    10021 tgttcgtagg ggatgtcgaa caggatgtgt tcgtcggcgg gaacagtcag ccacgcattg
    10081 tcggcaagtt cgcgttcgag ctggcctttt ccccagcttg aatagcctat gctgatcaag
    10141 gctttgtcaa ccgcaccttc gcgtgaaata ttttcaatca cgtctcggga agaagttagc
    10201 gcgatattgt ctgaaacgcc gatactgctt tgccagttgc cgatcggggt atgcacgaca
    10261 taacctcgct cgacttgcac cggcccgccc atcatcacgc tgtcgtgctg catccgcatg
    10321 gggatgtttt tgccggtggc ggaaaaaatc atgtccatcg taatcggaga gggtttgttg
    10381 atggcgatgc cgagtgcgcc gtcttcatcg tgtttgcaga tatagacgac cgattgtgaa
    10441 aaaaacgcgt cttccatatc gggcatggcg acgagaaagt ggttggagag gttcatatca
    10501 gcgtttgtcg ggattttcta tcgggttgga aatcatgcag gcgccggcaa gcaggttgcc
    10561 ggaagtatcg gcttgcaccc catcggtttg cggtatgccg ccgccgaaac aggaaaccgc
    10621 gcttaaggaa tttttcggcg agccttggat gattttatcg ctgtacacca aataggcgaa
    10681 ggttttgcgt ttggggtcgt aataacggac aatctgccgg ctcttgaacg cgaagctcgc
    10741 accgtgtttg aaaacttctt tcggtttgcg cacggcggtt tcgtcaaaag aaatcgaaga
    10801 tgccgtctga acgcacgaaa ccgatgcgtc ggacgcgtcc tcttccaaat tgaccatttc
    10861 cttcaagccg cctttttttg catacgaaat ataacaggca accccttgaa cgtcgggatc
    10921 gtcgaatcct tccacttcga tacggtcgtt tttgcccagt atgttgaaaa cggtacttgc
    10981 ccgtccgatt ttatcggttt cgccgctgcc gcaggcagtc agcaggacgg cggcagacag
    11041 cagtagcagt ctgttcatca aaatttcctt atcggttgaa tccccgtttt ccgtgaaggc
    11101 ggcagggctt gctttgtttg gaggggcgca cgttcccaaa atcgggcaac atacggtatg
    11161 cggcttaatg tgctaccctg tgtgtcggaa cacaatatcc gttgcggcat tgaaaatatg
    11221 acgcacaata taaggctgtt tgagccgatt gcaagtatcc gcgcaaagaa ggcgcaagat
    11281 tgggcaaacg gcattttcag acggcataac tgacagtata atccaaacgt ccggctgctt
    11341 tcgtgcagcc ttcagtatta tccaactttt tccgaaggtc gaaccatgcg ctacaaaccc
    11401 cttctgcttg ccctgatgct cgttttttcc acgcccgccg ttgccgccca cgacgcggca
    11461 cacaaccgtt ccgccgaagt gaaaaaacag acgaagaaca aaaaagaaca gcccgaagcg
    11521 gcggaaggca aaaaagaaaa aggcaaaaat ggcgcagtga aagataaaaa aacaggcggc
    11581 aaagaggcgg caaaagaggg caaagagtcc aaaaaaaccg ccaaaaaccg caaagaagca
    11641 gagaaggagg cgacatccag gcagtctgcg cgcaaaggac gcgaagggga taagaaatcg
    11701 aaggcggaac acaaaaaggc acatggcaag tccgtgtccg gatccaaaga aaaaaacgca
    11761 aaaacacagc ctgaaaacaa acaaggcaaa aaagaggcaa aaggacaggg caatccgcgc
    11821 aagggcggca aggcggaaaa agacactgtt tctgcaaata aaaaagtccg ttccgacaag
    11881 aacggcaaag cagtgaaaca ggacaaaaaa tacagggaag agaaaaatgc caaaaccgat
    11941 tccgacgaat tgaaagccgc cgttgccgct gccaccaatg atgtcgaaaa caaaaaagcc
    12001 ctgctcaaac aaagcgaagg aatgctgctt catgtcagca attccctcaa acagcttcag
    12061 gaagagcgta tccgccaaga gcgtatccgt caggcgcgcg gcaaccttgc ttccgtcaac
    12121 cgcaaacagc gcgaggcttg ggacaagttc caaaaactca ataccgagct gaaccgtttg
    12181 aaaacggaag tcgccgctac gaaagcgcag atttcccgtt tcgtatcggg gaactataaa
    12241 aacagccagc cgaatgcggt tgccctgttc ctgaaaaacg ccgaaccggg tcagaaaaac
    12301 cgctttttgc gttatacgcg ttatgtaaac gcctccaatc gggaagttgt caaggatttg
    12361 gaaaaacagc agaaggcttt ggcggtacaa gagcagaaaa tcaacaatga gcttgcccgt
    12421 ttgaagaaaa ttcaggcaaa cgtgcaatct ctgctgaaaa aacagggtgt aaccgatgcg
    12481 gcggaacaga cggaaagccg cagacagaat gccaaaatcg ccaaagatgc ccgaaaactg
    12541 ctggaacaga aagggaacga gcagcagctg aacaagctct tgagcaattt ggagaagaaa
    12601 aaggccgaac accgcattca ggatgcggaa gcaaaaagaa aattggctga agccagactg
    12661 gcggcagccg aaaaagccag aaaagaagcg gcgcagcaga aggctgaagc acgacgtgcg
    12721 gaaatgtcca acctgaccgc cgaagacagg aacatccaag cgccttcggt tatgggtatc
    12781 ggcagtgccg acggtttcag ccgcatgcaa ggacgtttga aaaaaccggt tgacggtgtg
    12841 ccgaccggac ttttcgggca gaaccggagc ggcggcgatg tttggaaagg cgtgttctat
    12901 tccactgcac cggcaacggt tgaaagcatt gcgccgggaa cggtaagcta tgcggacgag
    12961 ttggacggct acggcaaagt ggtcgtggtc gatcacggcg agaactacat cagcatctat
    13021 gccggtttga gcgaaatttc cgtcggcaag ggttatatgg tcgcggcagg aagcaaaatc
    13081 ggctcgagcg ggtcgctgcc ggacggggaa gaggggcttt acctgcaaat acgttatcaa
    13141 ggtcaggtat tgaacccttc gagctggata cgttgatcgg attgccgaaa cggtaggctt
    13201 tatgcaaatc gtgccgatat ggtgcgctgt tccgtgaagt ttgccgtttg ccgtacctgt
    13261 attttccggt ttttggaaat caagagagaa agttaaagaa gaatgtcgaa acctgttttt
    13321 aagaaaatcg cactttatac tttgggtgca atcagcggcg tggccgtcag tctggcggtg
    13381 cagggttttg ccgccgagaa ggacaggcgg gataacgaag tcctgccggt gcaatccatc
    13441 cgcacaatgg cggaggttta cggtcaaatc aaggcaaact actatcagga caaacccgat
    13501 gccgatttgt ttgaaggtgc gatgaagggt atggtggccg gtttggatcc gcattccgaa
    13561 tatatggata aaaaaggtta tgccgagata aaggagtcca ccagcggcga atttggcggc
    13621 ttggggatgg aaatcgggca ggaagacgga tttgtcaaag tggtttcgcc gattgaggac
    13681 acgcctgcgg aacgggcggg ggtgaaaagc ggcgatttca ttgtgaaaat cgataatgtt
    13741 tcgacacgcg gcatgacggt cagcgaagcg gtgaagaaaa tgcggggcaa gccgggtacg
    13801 aagattactt tgacgctgtc gcgcaaaaat gccgacaagc cgatagtcgt caacctgacc
    13861 cgtgccatta ttaaagtgaa aagcgtccgc catcacctga tcgaacccga ttacggctat
    13921 atccgcgtgt cgcagttcca agagcggacg gtcgaaagcg tcaataccgc cgcaaaagag
    13981 ctggtaaagg aaaataaagg aaaaccgctc aaggggctgg tgttggattt gcgcgacgac
    14041 cccggcgggc ttttgactgg cgcggtcggc gtgtcggcgg catttctgcc gtctgaagca
    14101 gtcgtcgtca gcaccaaggg acgcgacggc aaagaccgca tggtactgaa agccattcct
    14161 gaagattatg tgtacgggat gggcggcgat tcgttggcgg gcattcctgc cgagttgaaa
    14221 accataccga tgacggttct gattaattcg ggttcggctt ccgcgtcgga gattgtcgca
    14281 ggtgcattgc aggatcataa acgcgcggtc atcgtcggta cgcagagctt cggcaaaggt
    14341 tcggttcaga ctttgattcc tttgtccaac ggcagcgcgg tcaagctgac aacggcactg
    14401 tattatacgc cgaacgaccg ttctattcag gcgcagggga ttgttcccga tgtcgaagta
    14461 aaagataagg aacgcatttt tgaaagccgc gaggcggatt tggtcggaca catcggcaat
    14521 cctttgggcg gcgaggatgt gaacagtgaa acccttgccg tgccgcttga aaaagatgcg
    14581 gataagcccg ctgtaaaaga aaaaggtaaa aagaaaaagg acgaggattt gtcttcaagg
    14641 cggattccca accctgccaa agacgaccag ttgcggaaag ctttggattt agtcaagtcg
    14701 cccgagcagt ggcagaagtc tttggggctg gcggcgaaaa agccggtttc aaataaagat
    14761 aagaaagata aaaaagataa gaagtaggtt ttgacgatgc cgtctgaaag gatttcagac
    14821 ggcatattat gtttgaaagg acaggttatg agcgattttt taccggaatt tgaaccgtgc
    14881 gg
//
LOCUS       DAXHTR010000040        14826 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.040, whole genome shotgun sequence.
ACCESSION   DAXHTR010000040 DAXHTR010000000
VERSION     DAXHTR010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14826
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.040"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            306..1460
                     /gene="obgE"
                     /locus_tag="ACFNML_000829"
     CDS             306..1460
                     /gene="obgE"
                     /locus_tag="ACFNML_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HFC6392241.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTG
                     ENVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGEARSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDETVNPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQTRTAAFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRIEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1543..2592
                     /locus_tag="ACFNML_000830"
     CDS             1543..2592
                     /locus_tag="ACFNML_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6392242.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDTCMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3280..4701
                     /gene="cysS"
                     /locus_tag="ACFNML_000831"
     CDS             3280..4701
                     /gene="cysS"
                     /locus_tag="ACFNML_000831"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFC6392243.1"
                     /translation="MTTIYNTLTRQKELFAPIDPKNVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFAAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGATGHTCGHHHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAEFDLSENANGYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGSAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4872..5651
                     /locus_tag="ACFNML_000832"
     CDS             4872..5651
                     /locus_tag="ACFNML_000832"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6392244.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFGR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVVEYDYAAE"
     gene            5638..5982
                     /locus_tag="ACFNML_000833"
     CDS             5638..5982
                     /locus_tag="ACFNML_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392245.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPIWALVWLLICLPCLLIASKCLGWKGWRLLANVLACLAVRTLLSVPAT
                     LLIAFTLQDLLK"
     gene            5992..6522
                     /locus_tag="ACFNML_000834"
     CDS             5992..6522
                     /locus_tag="ACFNML_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFC6392246.1"
                     /translation="MESFELEDLTLWLVRDADEAEMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLQTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHAFQRVRCPCRAALVVPEHGGVPHGWAQRQADLWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6615..7730)
                     /gene="asd"
                     /locus_tag="ACFNML_000835"
     CDS             complement(6615..7730)
                     /gene="asd"
                     /locus_tag="ACFNML_000835"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFC6392247.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMQEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKTNF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLEMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
     gene            7907..8488
                     /locus_tag="ACFNML_000836"
     CDS             7907..8488
                     /locus_tag="ACFNML_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6392248.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
     gene            complement(8550..9404)
                     /gene="folD"
                     /locus_tag="ACFNML_000837"
     CDS             complement(8550..9404)
                     /gene="folD"
                     /locus_tag="ACFNML_000837"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFC6392249.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQDNLHMPCLAVVLVGGD
                     PASAVYVRNKKTACQKCGIKSLSYELPESTSQEELLALVDRLNADSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLTDEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            9627..9704
                     /locus_tag="ACFNML_000838"
     tRNA            9627..9704
                     /locus_tag="ACFNML_000838"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9662..9664,aa:Pro,seq:tgg)
     gene            9729..9805
                     /locus_tag="ACFNML_000839"
     tRNA            9729..9805
                     /locus_tag="ACFNML_000839"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9763..9765,aa:Arg,seq:tct)
     gene            9833..9908
                     /locus_tag="ACFNML_000840"
     tRNA            9833..9908
                     /locus_tag="ACFNML_000840"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9866..9868,aa:His,seq:gtg)
     gene            10044..10550
                     /locus_tag="ACFNML_000841"
     CDS             10044..10550
                     /locus_tag="ACFNML_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HFC6392250.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            10547..12325
                     /locus_tag="ACFNML_000842"
     CDS             10547..12325
                     /locus_tag="ACFNML_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HFC6392251.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLDVQIKWPNDLVVGRDKLGGILIETVRTGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLVELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            12438..13262
                     /locus_tag="ACFNML_000843"
     CDS             12438..13262
                     /locus_tag="ACFNML_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HFC6392252.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRAVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(14025..14813)
                     /locus_tag="ACFNML_000844"
     CDS             complement(14025..14813)
                     /locus_tag="ACFNML_000844"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFC6392253.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     AGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDAGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAGPVEARDKAQASTPTVGQPFWHSAEY"
BASE COUNT         3542 a         3777 c         4115 g         3392 t
ORIGIN      
        1 cgagtttatc ctagttatct aggacagccc caaaacttat tatatgtaac ggtttgaacg
       61 gacattccgt cgggtcggaa tgtcaaaaag gcgcgattgt accaaagaag ttggacataa
      121 ttttgtttgc aggctgaaga tttgcttaaa aattcattaa gatgggcgga acaaatagtt
      181 tgggtacggc gtgttgaaat accgcttatc ccttataata gcgtccgaaa ttctgttcag
      241 acggcatcaa aacacacggt aatccgtctg aacccccatt tgaaaccaac aaacaagagc
      301 attgaatgaa attcatcgac gaagcaaaaa tcgaagtcgc cgcaggcaaa ggcggtaatg
      361 gcgcaaccag tttccgccgc gaaaaattcg taccgcgcgg cggtccggac ggcggcgacg
      421 gcggcaaagg cggcagcgtt tgggcagaag ccgacgaaaa caccaacacc ctcgtcgaat
      481 accgcttcgt taaacgctac caagccaaaa acggcgaaaa aggccacggt tccgaccgtt
      541 acggcgcagg tgcggacgac atcgtcctca aaatgcccgt cggcaccctt atccgcgacc
      601 tcgacaccgg cgaaaacgtt gccgacctca cttatcacgg acagcgcgtc tgccttgcca
      661 aaggcggcaa aggcggcttg ggcaacatcc acttcaaatc ctccgtcaac cgcgccccaa
      721 aacaatccac gcccggcgaa gaaggcgaag cccgttccct gcaactcgaa ctcaaagtcc
      781 tcgccgatgt cggcttattg ggtatgccca acgccggcaa atccaccctg attaccgccg
      841 tatccgccgc acgccccaaa atcgccaact accccttcac caccctgcat ccaaacttag
      901 gcgtggtacg catcgacgaa aaccacagct tcgtgatggc cgacatcccc ggcctgattg
      961 aaggcgcggc agaaggcgcg ggtttggggc atcgtttcct caaacactta tcgcgcaccg
     1021 gattattgct gcacgtcgtc gatttggcgc ccttcgacga aaccgtcaac ccggccgaag
     1081 aagcacttgc tatcatcaac gaattgcgca aatacgatga agaactttac ggcaaaccgc
     1141 gctggctggt gctgaataaa ctcgacatgc ttgacgaaga agaagcccaa acgcgcactg
     1201 ccgccttcct cgaagccgtc ggctgggact accccaaacc cgacgaccgt ttccaatttg
     1261 acatggaaac cccgcgcctc ttccaaatca gcgcgctgac ccaccaaggc acgcaggaat
     1321 tggtacacca aatcaaccaa tacctgaccg agaaaaaacg tatcgaagct gaaaaagccg
     1381 aagcggagaa agcagcggca aatgttgaaa ttatcgaaca gcagcctaaa acggatacgg
     1441 gcgtgtttaa gccggagtga aaaaagaaag ggtttcagac gatatttgtt ctacaaaagg
     1501 ttgtttgaaa caattttaaa gcaagattga aatggattaa aaatgtcatc taaccaatat
     1561 atccagtcgc ttgagttgac aaactttacc gttcttccaa acgatacttt tgaattttct
     1621 gaaaatctca atgttattgt tgccgaaaac ggttgtggca aaacacattt gttaaaaatt
     1681 ttatacagcc tgctagaagt aacatctaac accaaaaaca gattactgaa aacagaattg
     1741 caaaaaagtt ttgccgataa attactgaat gttttccgtc ccgattcctt gggcaggttg
     1801 agcaaacgtt tgcaggggcg cgggcgtacg gaaatcgttt taaaactgca aaacggcact
     1861 acgcatagta gtctgaattt ttccagtaac tcatcatcgc aagtcaatgt tcaaagcatc
     1921 ggattgaaag aaaatgaata tactccgaca cctatcttct tacccagccg cgaattaatt
     1981 acactttgcc cttggttcac ttctttgtat caaaaccaaa gcatcccttt cgaagaaact
     2041 tggtttgata cctgtatgca attaaatcac ccgcttgcca aaggccccag agaaacaaaa
     2101 attcgagaat tgctcgaacc tattgagaac gcgatgggcg gaaaagtatc tgaagaacaa
     2161 ggaaggtttt atctatcgct gacaaatacg ggcggtaaga ttgaagcacc tttggtagca
     2221 gagggtttgc ggaaatttgt catgattgcc cggttaatcg caacaggggc attgctggat
     2281 aaaggctatc tattttggga tgaaccggaa gctaatctga atcctaaatt gatcaaagtg
     2341 gctgcccgta ttatttggtc tttatctcaa cagggtattc aggtttttat tgcaacacac
     2401 tctttattct tattgagaga attggaactt ttgaagcttg aaaataacac ggatttacct
     2461 gtacgttttt tctcgttgat tgcttccgat gatggaacaa aagtggagca agggaacagt
     2521 attaatgatt tgaacacatt gatattgtta gatgaaaaca ttatgcaggc agacaggtat
     2581 ttgaatattt gaggctaagc tatgactaca atcacagaaa agagattaac ctttgctttt
     2641 cctgaagatt attacgttac caagtatgac gaatgggaac attataaaat ttttcagaac
     2701 agttgtaatt tacgtaataa aattgataca aatgaaaagg gaaagaacgg gataaatcaa
     2761 agtgtagatg acgataatgg cagctcaggt gtagatataa tagcccttca tgaatcgaca
     2821 ctttggctaa tcgaaattaa agattattac cgacttgggc ttgagccaaa tgcccaatca
     2881 atagatgaaa aactttctga cctaccatat ttaatcgcaa gaaaaattcg agattcattg
     2941 gcaggattgg tttctgctaa atttaaagca gaaaaacagg aagaaaaaga tttttctcgc
     3001 ttggctttag attgtaacga aataaaaata gtccttcata ttgaaatgcc tagtataaga
     3061 agtaaacttt atccctcgtc atcagattta gcaaacttat taaaagataa gtttaaatta
     3121 tcagaattta caaaaaattt tgcaaactgt tatgcagaac caattttcac aaatatcagc
     3181 catattaata atccgcaact gcgtaatgtt ccttggtccg tatctacagg aacagaacag
     3241 aaattatcat ccgagcaaca aagattaatc cataatccca tgaccaccat ctacaacacc
     3301 cttacccgtc aaaaagaact cttcgccccc atcgatccga aaaacgtgcg tatgtacgtt
     3361 tgcggcatga ccgtttacga ctattgccac ttaggccatg cccgtgtgat ggtggtgttt
     3421 gacatgattg cccgctggtt gcgcgagtgc ggctatccgc tcacttatgt acgcaacatc
     3481 accgacatcg acgataaaat cattgcccgt gcggctgaaa acggcgagac tatcggcgaa
     3541 ctgaccgcgc gtttcattca ggctatgcac gaagatgccg atgctttggg cgtgttgcgt
     3601 ccggatattg agccgaaggc aacggaaaac attccgcaaa tgattgccat gattgaaacc
     3661 ctgattcaaa acggcaaggc atatcctgcc gcaaatggcg acgtttacta cgccgtgcgc
     3721 gagtttgccg cttacggaca actgtcgggc aaatcgttgg acgacctgcg cgcgggcgaa
     3781 cgcgtggaag tggacggttt caaacgcgat ccgcttgatt ttgtattgtg gaaagcggcg
     3841 aaagcaggcg aaccggcgtg ggaaagcccg tggggcaacg gccgtccggg ttggcacatt
     3901 gaatgctctg ccatgagtga aaacctgttc ggcgatacct tcgacatcca cggcggcggc
     3961 gcggatttgc agttccctca tcatgaaaat gaaatcgccc aaagcgtcgg cgcgacgggg
     4021 catacctgcg gtcatcatca cgcgcaaacc caccacgggc aaagcatcgc cagccacgtc
     4081 aaatattggc tgcacaacgg ctttatccgc gtggacggcg aaaaaatgtc caaatcactg
     4141 ggcaacttct tcaccatccg cgaagtgttg aaacaatacg accccgaagt cgtgcgcttc
     4201 ttcatcctgc gcgcccacta ccgcagcccg ttgaactatt ccgacgcgca tttggacgat
     4261 gcaaaaggtg cgctgacccg tctgtacacc accttgaaaa acacgccggc ggctgagttt
     4321 gatttatccg aaaacgccaa cggctacacc cgccgcttct acgccgccat gaacgacgat
     4381 ttcggtacgg tcgaagcggt tgccgtgttg ttcgaactgg caggcgaagt gaacaaaacc
     4441 aatgacgcac agctcgccgg ctgcctgaaa gccttgggcg gcatcatcgg cctgctgcaa
     4501 cgcgacccga ccgagttcct gcaaggcagc gcggcttcag acggcctctc caacgaagaa
     4561 atcgaagact tgatcgcccg gcggaaacag gcgcgcgccg ataaaaactg ggcggaatcc
     4621 gaccgcatcc gcgaccttct gaacgaacac aaaatcattt tggaagacaa cgccggcggc
     4681 acgacttggc ggcgcggttg aactgtatcg gaaagggcaa aagtaccgcc ttttgctctt
     4741 tttccgtttt cagacggcat ttcccgcttg aagccccgcc gttttcagca ggcagggttg
     4801 aatgggaaat gccgggcggc gcaccaggtg ccgttttgtg tatgggaaca ttgtgagaac
     4861 ttatttttat tatgcttaaa atcatttccg ccaacgtcaa cggcatccgc tccgcctaca
     4921 aaaaaggttt ttacgaatac atcgccgcat cgggcgcgga cattgtctgc gtgcaggaac
     4981 tcaaagcgca ggaggctgat ttgtctgccg atatgaaaaa tccgcacggg atgcacggcc
     5041 attggcattg tgccgagaag cgcggttaca gcggcgtggc ggtgtacagc aaacgcaaac
     5101 ccgacaatgt gcaaatcggt atgggcattg aagaattcga ccgcgaaggg cggtttgtgc
     5161 gttgcgattt cggcaggttg agcgttattt cgctttactt gcccagcggc agcagcgcgg
     5221 aagaacgcca gcaggtgaaa taccgtttcc tcgatgcgtt ttaccctatg ctcgaagcga
     5281 tgaaaaacga agggcgcgac attgtcgttt gcggtgactg gaacatcgcc caccaaaaca
     5341 tcgacctgaa aaactggaaa ggcaaccaga aaaattcagg tttcctgcct gaagagcgcg
     5401 agtggatagg caaggttatc cacaagctcg gttggacgga tatgtggcgc acgctttatc
     5461 ccgatgtgcc gggctacact tggtggagca accgagggca ggcgtatgcg aaagatgtcg
     5521 ggtggcgcat cgattatcag atggttacgc ccgaacttgc cgccaaagcc gtgtccgcac
     5581 acgtttataa agatgaaaaa ttttccgacc atgcgccgct ggtcgtggag tatgactatg
     5641 ctgccgaata aagttttggg taaatatgat tggaatgtgg acggaaaaac aggaataggc
     5701 gcggcatggg ttgtcgctgc gtttatcctg cccatattgg tgtgggcgat atttatgttg
     5761 tcgcggatgc aaggctggct tgcgccgacc aaggcaaacc cgatatgggc gttggtgtgg
     5821 ctgctgattt gcctgccctg cctgctgatt gcgtccaaat gtttgggttg gaaaggctgg
     5881 cggctgttgg caaacgttct tgcctgcctc gccgtgcgca ccctgttgag tgtgcctgcc
     5941 acgctgctga ttgcgttcac gctgcaagac ctgctcaaat aggcgcggac aatggaaagt
     6001 ttcgagcttg aggatttgac gctgtggctg gtacgcgatg cggatgaagc ggaaatgtgg
     6061 atagaccgct gggcgatcag ttatcccgtc gtgcagatgt ccgaagcgtc ggccggtcaa
     6121 agcatagggg aatggcaggc agggcttcag acggcatttg aacgcatacg cggcaaatac
     6181 gtcgccgttg tcgcacacgg tgcgggcgcg gccgcatttt tggcgtggct gtatcaggca
     6241 gacatcctga cacggaagaa aattgccaac atcatccttg taccgcagcg tcccgatatt
     6301 tttcccgacg atgcggaaca cgctttccaa cgcgtccgct gtccctgccg tgccgcattg
     6361 gttgtacccg aacacggcgg cgtgccgcac ggttgggcgc aaagacaggc ggatttgtgg
     6421 aacgcccgcc tgttggtttc cccgcattcg ggcagtttga acggtatgct cggcggctgg
     6481 cagtggggta tgaagctgat gcaggaaatg ttgctggcgt gaacgcttgc cttatcggaa
     6541 tttcggttaa ctggggttgc aaggaaaatg ccgtctgaaa caggcttcag acggcatttc
     6601 cattcaaaca gggcttacag gctgcccaac acgatacgca gcacgcggcg cagcggctcg
     6661 gccgcgcccc acaagagctg gtcgccgaca gtgaacgcgc tgatgtattc gccacccatc
     6721 tccagtttgc ggatgcgtcc gacagggacg gacagcgtgc cggtaacttt ggcaggagtc
     6781 agctcgtgga tgctggcttc tttttcattg gggatgactt tcacccagtc attcgcgcct
     6841 gccaaaatcg tttcgatttc ggaaacaggc aggtcttttt tcaacttcag agtgatggct
     6901 tggctgtggc agcgcatcgc gccgacgcgg acgcacaggc cgtcaatcac ggttggattg
     6961 tcgctgcggc cgaggatttt gttggtttcc acgccgcctt tccattcttc tttggactgg
     7021 ccgttgccca aatccacgtc aatccacgga atcaggctgc cggcgagcgg tacgccgaag
     7081 ttggttttcg gatagtcttc gctgcgcagg aaatcggata ctttgcggtc gatgtcgaga
     7141 atcgagccgg caggatcggc aagctcgtcc gccacttggg cgtgaaccgc gcccataccg
     7201 ctgatgagtt cgcgcatatt tttcgcgccc gcgccggaag cggcctgata ggtcatactg
     7261 gtcgcccatt cgaccaaatc gttttggaac aggccgccca aagccatcaa catcagggag
     7321 acggtgcagt tgccgccgat gtagtttttc acgccgtttt tcaaaccgtt gtcgatgacg
     7381 ttgcggttga cggggtcgag gacgataatc gcgtcgtctt tcatacgcag cgaggatgcc
     7441 gcgtcaatcc agtaaccgtt ccagccgctg tcgcgcaggg cttggaagac ggatttggtg
     7501 taatcgccgc cttggcaggt aacgatgatg tccatttttg ccagttcggc aacgttgttc
     7561 gcgtccaata atgttttagc cgcctgaccg aaatcagggg ctgcgccgcc gacgttggaa
     7621 gtggtaaaga aaaacgcttc gggaatgtgg gcgaagtcgt tttcttcttg catacgctgc
     7681 atcaaaaccg aaccgaccat gccgcgccag ccgacgaaac ctactttcat tgtgttgctc
     7741 cttgaggaaa aaatcgttaa caaaattatg tatggcgtaa aggaagcgtt ttaacgccgt
     7801 attgggaaaa tgtaaagttc tttatcggga tttctttaag tattggcgtt ttcggtgtcg
     7861 tggcggtaga atcggggtca gttaagaaca attatcagga acggatatgg aagaaaaaaa
     7921 cgactacacc gacgcggcat ccgacaaccg caacgggcag gaaattgaag tcggaattgc
     7981 cggcgcaggc gaccggatac ttgccgcgct gctgaaccaa ttgtttacct ttttggtttt
     8041 gttggtgccg tttgccggac tgattgcttt cgccatcaag aatgaaggca ggattggcgg
     8101 cagtgaggaa atattcgggc tgcttttggg catgacttcc ttttgggtag gactggcggg
     8161 cattttggtt tataccgtcg ttcaaatcta ctatatgagt cgggacgggc agtcattggg
     8221 taagaagatc atgagaatca gggtgttgaa aaccgacggc cgcaatccgg gttttgtcgg
     8281 cacggttttg gtacgcgaaa tcgcatggtc ggttttggtt gccattattg ccgccgttat
     8341 cggtcttgca gtaggtgaca acggagaaaa cgccatcaac ctgctggcat tccttgccaa
     8401 ctttgtcctg ctctttatgg tcaaacgcga ccgccgcacg ctttacgaca tactggcgga
     8461 tacggttgtc gtcaagctgc ccaaataaac ggacacggca aaatgccgtc tgaaagcctt
     8521 tcagacggca tttttatctt cagaaccgct caagcatcgt gcagtgaagc cgcgtgcagg
     8581 gtgttttcca tcaatgtggc aatcgtcatc ggacccacgc cgccgggaac gggcgtaatc
     8641 atcgccgccc gttcttttgc cgtttcaaat tccacgtcgc cgcacaggct gccgtcgtcc
     8701 aaacggttga tgcccacatc gataacgacc gcgccgggtt tgatccattc gcctttgaca
     8761 aagttcggaa tgcctacgcc gaccaccaaa atatcggctc cggcaacctc gtctgtcaga
     8821 ttttcggttg cgctgtggca gaccgttacc gttgcgcgcg ccagcagcag ttccaaagcc
     8881 tgcgggcggc cgacgatatt cgacgcgccg accacgaccg cttttttccc cttcggatca
     8941 atgccgtaag cttccaaaag cgtcatcacg cccttgggcg tacacgggcg catcagcggc
     9001 attttgaccg ccagcctgcc gacattgtaa ggatggaagc cgtccacgtc cttatccggc
     9061 gaaatacgtt ccaaaaccgc ctggctgtcg aggtgcttcg gcagcggtag ctgaaccaga
     9121 ataccgtcca cttcggaatc ggcattcagg cggtcgacca gtgccagcag ttcttcctgc
     9181 gatgttgatt cgggcagctc gtaagacagt gatttgatgc cgcatttttg gcaggcagtt
     9241 ttcttgttgc ggacataaac cgcgctggca gggtcgcctc cgaccaaaac cacggccagg
     9301 caaggcatgt gcagattgtc ctgttggcgt tgcgccaccg cttcggcaac cgcctgcagg
     9361 cgtttttgcg aaacttcttt accattgatc agttgtgccg acatatccgt ccttttgtta
     9421 aacaggtaga aaatgctgaa aattatcgca ttttccgttt gtttttacag caggatgccg
     9481 aaaataaatt tgccctgaaa taaaatacgg ctgtttggac ggattaaata ttttttcaaa
     9541 tcaaaaaact atatttttca tcttggtgtc ttgaaaataa atattgacgc atctttccct
     9601 gagttgtata gttcgcttct tcaagtcggg gcgtagcgca gcccggttag cgcatctgct
     9661 ttgggagcag agggtcgtga gttcgaatcc caccgccccg accaatcaat cagcacgaat
     9721 cggcatgagc gcccgtagct caaccggata gagcaccgac cttctaagtc gggggttaca
     9781 ggttcgattc ctgtcgggcg tgccaagaat ttgattgaaa accgttttta tggtggctgt
     9841 agctcagttg gtagagcccc ggattgtgat tccggttgtc gtgggttcga gccccatcag
     9901 ccaccccaga taaaggcccg tacttttcgg tacgggtctt ttcccgtttg cgcgtactgt
     9961 ccgcgccgcc ggctttgcct ttcgcatatg cttctgtatc atgtgggttt ttctgtcttt
    10021 tatggttttt gtcggaggct ttgatggttg acgcttggtc tgtccctgat tttgaatcga
    10081 aaatctgtcc gcccgaggcg ttggcggcgc gtttggcgtt gttgccgcgc ccgctggtgt
    10141 ttaccaacgg ctgtttcgac atcctccaca gggggcacgt tacttatctg gcgcaggcgc
    10201 gttcgatggg gggcgcgttg gtgctggcgt tgaataccga tgcttcggtg cggcgtttgg
    10261 gcaagggcgg cgaccgcccg attaatcctt tggagaaccg tgccgccgtt gccgccgctt
    10321 tggaaagtgt ggatttggta acgtggtttg acggggatac gccggcggcg ttgattgagg
    10381 cggtcaaacc tgagattttg gtcaagggcg gcgattgggc tgcggataag attgtcggtg
    10441 cggcagaaac gttggcgcgc ggcggtcagg tgttttcaat tccgtttctg caccagactt
    10501 cgacaacgaa gactttggca aaaatccgtg cggcagaggg cggaaaatga cggttttgaa
    10561 gctttcgcac tggcgggtgt tggcggagct tgccgacggt ttgccgcaac acgtctcgca
    10621 actggcgcgt atggcggata tgaagccgca gcagctcaac ggtttttggc agcagatgcc
    10681 ggcgcacata cgcgggctgt tgcgccaaca cgacggctat tggcggctgg tgcgcccatt
    10741 ggcggttttc gatgccgaag gtttgcgcga gctgggggaa aggtcgggtt ttcagacggc
    10801 attgaagcac gagtgcgcgt ccagcaacga cgagatactg gaattggcgc ggattgcgcc
    10861 ggacaaggcg cacaaaacca tatgtgtgac ccacctgcaa agtaagggca gggggcggca
    10921 ggggcggaag tggtcgcacc gtttgggcga gtgtctgatg ttcagttttg gctgggtgtt
    10981 tgaccggccg cagtatgagt tgggttcgct gtcgcctgtt gcggcagtgg cgtgtcggcg
    11041 cgccttgtcg cgtttaggtt tggatgtgca gattaagtgg cccaatgatt tggttgtcgg
    11101 acgcgacaaa ttgggcggca ttctgattga aacggtcagg acgggcggca aaacggttgc
    11161 cgtggtcggt atcggcatca attttgtcct gcccaaggaa gtagaaaatg ccgcttccgt
    11221 gcaatcgctg tttcagacgg catcgcggcg gggcaatgcc gatgccgccg tgctgctgga
    11281 aacgctgttg gtggaactgg acgcggtgtt gttgcaatat gcgcgggacg gatttgcgcc
    11341 ttttgtggcg gaatatcagg ctgccaaccg cgaccacggc aaggcggtat tgctgttgcg
    11401 cgacggcgaa accgtgttcg aaggcacggt taaaggcgtg gacggacaag gcgttttgca
    11461 cttggaaacg gcagagggca aacagacggt cgtcagcggc gaaatcagcc tgcggtccga
    11521 cgacaggccg gtttccgtgc cgaagcggcg ggattcggaa cgttttctgc tgttggacgg
    11581 cggcaacagc cggctcaagt gggcgtgggt ggaaaacggc acgttcgcaa ccgtcggtag
    11641 cgcgccgtac cgcgatttgt cgcctttggg cgcggagtgg gcggaaaagg cggatggaaa
    11701 tgtccgcatc gtcggttgcg ccgtgtgcgg agaattcaaa aaggcacaag tgcaggaaca
    11761 gctcgcccga aaaatcgagt ggctgccgtc ttccgcacag gctttgggca tacgcaacca
    11821 ctaccgccac cccgaagaac acggttccga ccgctggttc aacgccttgg gcagccgccg
    11881 cttcagccgc aacgcctgcg tcgtcgtcag ttgcggcacg gcggtaacgg ttgacgcgct
    11941 caccgatgac ggacattatc tcgggggaac catcatgccc ggtttccacc tgatgaaaga
    12001 atcgctcgcc gtccgaaccg ccaacctcaa ccggcacgcc ggtaagcgtt atcctttccc
    12061 gaccacaacg ggcaatgccg tcgccagcgg catgatggat gcggtttgcg gctcggttat
    12121 gatgatgcac gggcgtttga aagaaaaaac cggggcgggc aagcctgtcg atgtcatcat
    12181 taccggcggc ggcgcggcaa aagttgccga agccctgccg cctgcatttt tggcggaaaa
    12241 taccgtgcgc gtggcggaca acctcgtcat tcacgggctg ctgaacctga ttgccgccga
    12301 aggcggggaa tcggaacata cttaaaaaat acaccctttt tcaacacggg gcggcatccg
    12361 tccgaatccg tccggattct gccgcccgac agactgccgc ccaggcgggc ggggtttcaa
    12421 acattgagga aattctaatg aaatggctct tcaccctttt ggcggtactc aatattgccg
    12481 tattcggcgg cacggtgggc tataaattgg ccgttaaagc gacgggcggc gtgccggaaa
    12541 accgggccgt cgaaaacacg ccccccgcaa cgcccgccgc cgacaatgcg gcggcatccg
    12601 tggaggatac ggcggcactg ctcaaaccgg gcgacatcct gaccgaagag caggccgagc
    12661 agttgcgctt gaaaaaggaa gcggaacaga aaaaactgag ggagaaaaaa cagcgtgaag
    12721 aaaaagcccg ccgcgaaaaa ctcgccgccg aaaaggcgca ggcggaacgc gaaaacggcg
    12781 cggcggatgc cttatgcgcc gcgcaggcaa gcctcacgat ggacgaagac gactaccacc
    12841 gcatcaaagg gcttttgggc aaatggtcgc acgttgccag caggagcgtc gaaaaacgca
    12901 ccgccaaagc caaacctgcc gacaaaacct accgcgtcgt cctgcccgtt tccgccgatg
    12961 ccgaaaatca ggcggcggag ctgtctgcca aaggtttcaa ccccataccg tttgacggcg
    13021 cattgagttt gggtgtcggc aacagccggg aaaacgccca agccctgcaa aaccggcttg
    13081 ccggcgccgg attcggcggg gcgcatattg tcgaacactt tgccgaagcc gacaggcagg
    13141 acgattcttt gagcgtgtcg cgtatgacgg ttttgtttac cggcgtgaat gccgccgatg
    13201 cggacgaaat tcgtaaaatc acgtccctat acggcaaact gaacctcaag tcttgcaaat
    13261 aggcgcgaaa gccggacgat aaacgccaaa cccaccgcga aggtgggttt ggcgtttcgg
    13321 ttttgagtat ctgatatata aaaagggaat gtatgtctcg tatatgccga ctccaagtgt
    13381 gaaagtgaat tgtgagggaa tacagtgttt aatggttcgg aatggacgac ttccgagtgg
    13441 ttttgattat acgctaataa taataattat caataaaaat ttaaaaataa atgcggattt
    13501 tttgtaatac gatgaaaaat aaacaaatta acctgtccgc cgatgtttgt gattcgggtc
    13561 ggcaaacttt cattgtgtcg gacaaatcgg ggtcggcagt catagctgag ggcttcgtgc
    13621 ctgacggaaa taagcctgcc gtgtttgaac atccgtccga tttggcgcgg acagggttgt
    13681 cattcggggg tgtttgagcg tggattcggg gatgcgaaag cggtaaaacc gttgcggatg
    13741 aaggcttacg gacggcttta ggtattttta agtgtattca aacaacaaca aaacgcttct
    13801 tctattgccg tatttagggc ttgcgcggca gcctgcacac cgtggaggcc gagtgtttcg
    13861 ttggttgcgg catcgggacg ggcggcaagg tttttcggcc ggtttttcca ttttttccga
    13921 caaagccgtg atgccgggga atggcggttt gtccgtttca ggccgccgcc gttttggacc
    13981 gcgatgccgt ctgaagcctt cagacggcat ttttgccgcc tttttcaata ttccgccgaa
    14041 tgccaaaacg gttgtccgac tgtcggcgtg ctggcttgcg ctttgtcgcg tgcttcgacc
    14101 ggtccggctt caaatgccag ccgtccggat tcgacggcga gtgcgaaggc gcgtgccata
    14161 ttgaccggat cgccgctgcg ggaaacggca gtattcaaaa gcacgccgtc aaagccccat
    14221 tccatcactt gtgccgcctg tgagggcaaa cccaagcccg cgtcgataat cagcggcgtg
    14281 tcgggcaggc gttcgcgcag gacgttcaac gcgtaggcgt gaaccgcgcc caaacccgtg
    14341 ccgatcgggg ccgcccacgg catcaacgcc tgacagcccg cgtcgagcag gcggcggcag
    14401 gcaatcaggt cttcggtgca ataaggcagc actttgaagc cgtctttaat caggatttcc
    14461 gccgcttcga caagctggaa cacatccggc tgcaaggtgt cgtcatctcc gatgagttcc
    14521 aattttatcc aatcggtttc aaacacttcg cgcgccattt gcgccgtcgt taccgcttcc
    14581 tgcacgcttt ggcagcctgc cgtgttcggc aggacgggaa cgccggtttc ttgaagcagc
    14641 gaccaaaacc cctgaccgtg cgcctcgccg ccgcttcccg cgcggcgcaa tgagacggta
    14701 atcatcgcgg gttgggcgat acggacggat tgtttgagga tttcaggggt cgggtaggca
    14761 gccgtgccga gcagcagccg tgaagggaaa gtttctccgt atagggtgag cataatgggt
    14821 tccttt
//
LOCUS       DAXHTR010000041        14812 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.041, whole genome shotgun sequence.
ACCESSION   DAXHTR010000041 DAXHTR010000000
VERSION     DAXHTR010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.041"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(38..763)
                     /gene="aat"
                     /locus_tag="ACFNML_000845"
     CDS             complement(38..763)
                     /gene="aat"
                     /locus_tag="ACFNML_000845"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFC6392254.1"
                     /translation="MRIPLLAPDNYAFPDPAYALARCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGSV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVACKGL"
     gene            complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACFNML_000846"
     CDS             complement(833..1267)
                     /gene="fur"
                     /locus_tag="ACFNML_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFC6392255.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            1486..1863
                     /locus_tag="ACFNML_000847"
     CDS             1486..1863
                     /locus_tag="ACFNML_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6392256.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMTKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            1873..2682
                     /gene="dapB"
                     /locus_tag="ACFNML_000848"
     CDS             1873..2682
                     /gene="dapB"
                     /locus_tag="ACFNML_000848"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFC6392257.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(2946..3356)
                     /locus_tag="ACFNML_000849"
     CDS             complement(2946..3356)
                     /locus_tag="ACFNML_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392258.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPMPNYSEWVKIKFKQFSYLKFIYGYATKNQDKNIKNVLELGELKQDDEILDYGGA
                     LEVIGGRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            complement(3358..3603)
                     /locus_tag="ACFNML_000850"
     CDS             complement(3358..3603)
                     /locus_tag="ACFNML_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392259.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNEAELARVGLRL
                     KYNGLLKEEFKQLKFHKSSRTNLDSKNLKLIVFEKVK"
     gene            complement(3611..4783)
                     /gene="lpxB"
                     /locus_tag="ACFNML_000851"
     CDS             complement(3611..4783)
                     /gene="lpxB"
                     /locus_tag="ACFNML_000851"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFC6392260.1"
                     /translation="MWGQTNMADKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     VGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRSGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHPMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLERYPAACFLLPAATEATKRRLAEVLQRPEFAGLPLTVIDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQSEAKPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(4831..5823)
                     /locus_tag="ACFNML_000852"
     CDS             complement(4831..5823)
                     /locus_tag="ACFNML_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HFC6392261.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSEDGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPPDLAQFAVMLENGTKM"
     gene            6318..9074
                     /locus_tag="ACFNML_000853"
     CDS             6318..9074
                     /locus_tag="ACFNML_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HFC6392262.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYEGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAQLDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRMVQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTAGQEARADKAET
                     RNNGNRRRNERGDRAAERANEAEIQDRNIQPAATVADAAPSETEVQTGKRRRNGSRNE
                     RGQTALETAAVAETAVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPLI
                     ATPVQTASSAVSNGDALVYDAAEKIRRAAAILPEGATPKASAQEMPSETATFTAAAEQ
                     ARETAQTGGLVLIETDPAALKAWAAQPEVQTGRGLRRSEQPKPSEAATVPAEEMIQVE
                     TRQG"
     gene            complement(10023..11015)
                     /gene="pdxA"
                     /locus_tag="ACFNML_000854"
     CDS             complement(10023..11015)
                     /gene="pdxA"
                     /locus_tag="ACFNML_000854"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFC6392263.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAPGEGELEVLHIPTAEAVAAGRLNPANAAYVLQLLDTA
                     LAGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLR
                     VALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGESGH
                     LGHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKY
                     HSFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
     gene            11175..12602
                     /locus_tag="ACFNML_000855"
     CDS             11175..12602
                     /locus_tag="ACFNML_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFC6392264.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            12927..>14812
                     /gene="mnmG"
                     /locus_tag="ACFNML_000856"
     CDS             12927..>14812
                     /gene="mnmG"
                     /locus_tag="ACFNML_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFC6392265.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGDRISGVITAMGVEFKALAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELNLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIAGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSERLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFK"
BASE COUNT         3597 a         4591 c         3648 g         2976 t
ORIGIN      
        1 ccaccatatt tgaacctgac cgaagcataa gccgccatca aagccccttg cacgccactt
       61 cgcgccgccc gatttcctct ttcaacggca cggcgttcaa catccgcaga cgttcggcaa
      121 aatccgcaaa cggcagcagc tccgaaccga aacggcgcat atgttccgtg tcctgctggc
      181 agtctatcag ttccacaccc aaatccgcca aaaacggcac ggcgcaggca aacgcgattt
      241 tcgacgcatc cggttgcaac gcaaacatcg attcgccata aaacacgctg ccgatctgca
      301 cgccgtaaaa gccgcccgcc aacctcgttt caccgctttc atcgggataa tggcactcga
      361 aagaatgcgc gtgtcccatt tcgtgcagct tcaaatatgc cgtctgaaac tcgggcgcaa
      421 tccaagttcc gtcctgattc gggcgcgccg ctgccgcaca atgcgcgacc acttccgcaa
      481 aacagccgtt gaccgcaacc cgatagctgc cgttgcgcag cgttttcgcc agcgagcgcg
      541 gaacgtgcag acgttcggga acaatcaccg cacgcgggcc gaccgcatac caaaaaaacc
      601 aaccgtcccg agaaaaccac ggaaacacgc cgttccgata cgcctcgagc agccgccccg
      661 catccaaatc gccgctcacg ccgaccagcc cgtcgcaccg ggccaaagca taggcaggat
      721 cgggaaaggc ataattgtca ggggcaagca gcggaatacg catggcagtt ctccaaaaac
      781 acagacggca tccaaaatca tgaatgccgt ctgaacaaca aaccgtccgg atttaacgtt
      841 tgcccttggc ctgacagtcg ctgcacacgc cgtacatata aagcgcgtga tcgacgatgc
      901 ggtagccgtt ttcctcagcg attttgtctt gcagggcttc gatttcggga ttgtggaatt
      961 ccgttacctc gccgcacttc acgcagacga tatggtcgtg gtggtcgccc ttgtccaact
     1021 cataaaccgc cttgcccgtt tcaaaatggt ggcgttgcag aatgcccgcc tgctcaaact
     1081 gggtcagcac gcggtaaatc gtcgccacac cgatttccac accctcttcc aacaaaatgc
     1141 ggtacacatc ttccgcactc aaatgctctt ccgcatgcgt ctcgaacaaa tccaaaatct
     1201 tcaaacgcgg gccggtaacc ttcagaccgc tgtctttcag ttgtgcaata ttgttgaatt
     1261 tttccataat attcaatacc cctgtaaaac aatagacgtt ataatacgca atttcggcct
     1321 gtttgcccac tatcgcacca tagcagtttg caatagtaaa accgcaccgg cggccgatgc
     1381 gcggacaggg cggataccgc ttaatcgtat gattatcgtt cgcttttata aaatattcaa
     1441 gcagtgttac actacccatc ccgatttgca cagaaaggca tttccgtgaa caaaaccctc
     1501 atcctcgccc tttccgccct cctcggcctt gccgcgtgca gcgtcgaacg cgtttcactg
     1561 ttcccctcgt acaaactcaa aatcatacag ggcaacgaac tcgaacctcg cgccgtcgcc
     1621 tccctccgcc ccggtatgac caaagaccaa gtcctgctcc tgctcggcag ccccatactg
     1681 cgcgacgcat tccataccga ccgctgggac tataccttca acacctcccg caacggcatc
     1741 atcaaagacc gaagcaatct gaccgtctat tttgaaaacg gcgtgctcgt ccgcaccgaa
     1801 ggcaacgccc tgcaaaatgc cgccgaagcc ctccgcgtaa aacaaaacgc agacaaacaa
     1861 taaggaacac acatgacacc gctcaaaatc gccatcgccg gcgcaaacgg ccgtatgggg
     1921 cgcgtcctcg tcgaagccgt caacaaccat cccgacaccg tcctttccgg tgcgcttgaa
     1981 cactcaggct cagaagccct cgggctggac gcaggctacg ccgtcggact caaaaccggc
     2041 atcgccattt cagacgacgt tgacgccgtt ctcgcacaaa gcgacgtact catcgacttc
     2101 acccgccccg agcccaccct caaacacctg caaaaatgcg ttgaaaaaca agtcaacatc
     2161 atcatcggca caacaggctt cgacgatgcg ggcaaagccg ctatccacac tgccgccgaa
     2221 caaacaggca tcgttttcgc cgccaacttc agcgtcggcg tcaacctcac cttccacatc
     2281 ctcgacaccg tcgcccgcgt gttaaacgaa ggctacgaca tcgaaatcat tgaaggccac
     2341 caccgccaca aagtcgatgc ccccagcggc accgcgttac gcatgggcga agtcatcgcc
     2401 ggcgcgctcg gcagagacct caaacaatgc gccgtttacg gccgcgaagg ccacaccggt
     2461 ccgcgcgatc cgtcgaccat cggctttgcc accgtccgcg caggcgacat cgtcggcgac
     2521 cacaccgccc tcttcgccac cgacggcgag cgcgtggaaa tcacccacaa agccggcagc
     2581 cgcatgacct ttgccgccgg cgccgtccgc gccgcagttt gggtaaacgg caaaacgggt
     2641 ttgtacgata tgcaggacgt actcgggctg aacaaccgtt aacccccata caaaatgccg
     2701 tctgaaaaga tattgttcag acggcatttt gccgacaggc tccgtatcgg catatcaatg
     2761 tttcaacaca cgggacgata cataaagcgt cgccctatgt gtcgtcctga tttggaaggg
     2821 attacgcccc tcccaaataa agtctgattc taccgccccg aaggacagat gtccgagtgg
     2881 cggggtttca accgaaaagg aaatacgata aagttgcttg cgcggcatga caagttacgg
     2941 ctcgtttaat taaaactctc ctgatctaaa aattctaact ctatttcccg gcaaactata
     3001 tctatactaa aaccggtcgg aagatcatac ctaccgccta tcacttccag cgcacctcca
     3061 taatccaaga tttcatcatc ctgctttaat tctccaagtt ccaatacatt tttgatattt
     3121 ttatcttggt tcttcgtggc gtatccatag ataaatttca gatagctgaa ctgcttgaat
     3181 ttaattttta cccattctga ataattgggc atagggatct cttgaggaaa gtccggataa
     3241 tccacattct ggacgatgat atggaattca tcgtttttca aagtatagtt taatagagtg
     3301 ccctcatata tatacagaaa ttgataatct ttttttggaa tggtataagt tgtcatatta
     3361 ttttaccttt tcaaaaacaa tgagtttaag attttttgag tccaagttgg ttctactact
     3421 cttatgaaat ttcagttgtt taaactcttc ctttaacaga ccattatatt ttaaccttaa
     3481 tcccactcga gccagttctg cttcatttgg aattcctctt aaatacttat tgtttgatgt
     3541 gccattaatc acaatttctc cccctttttt tgtaatacgt gctgcttcgg gaaaataatc
     3601 cattaaccgc tcaacatccc gcctcttcca aaaccgcgcg cgcggccaaa tccgccgtat
     3661 cttttttcaa cagcaggtgc aacgccctga aatcctgttg cagcgcggca accttatcgg
     3721 ggtgttcgta ccagtccgcc aacgccgccg ccagtttttc cggttttgct tcagattgca
     3781 ataattccgg cacagcctcc ttacccaaca ggatattcgg caggccgaca tgcggcactt
     3841 tgattttgcg tttcacataa gcataagtca gcggcgaaat cttgtagctg atgaccatcg
     3901 gacgcttgca caacgccacc tccaaagtcg ccgtaccgct cgttaccagc accgcatccg
     3961 ccgccctgca cactgtttca gactgtctgt cgattaccgt cagcggcaat ccggcaaact
     4021 ccggccgctg caaaacttcc gccaaacgcc gcttcgtcgc ctccgttgcg gcaggcagca
     4081 ggaagcatgc ggcaggatag cgttccaaca acaataatgc cgtctgaaaa aacaccggcg
     4141 ccatatagtc gatttcgctg acgcggctgc cgggcagcag ggcgaatacg gggatgccgg
     4201 catccacgcc caaagtttgc cgcgccgttt cacggtcgtc ttccaagggc ataagctgcg
     4261 ccatcggatg accgacaaac tccgcacgtc cgcccgcatc gagataaagc tgcggctcca
     4321 tcgggaacag gcacaacacg cggttgacct gatgcacgat tttgcccaca cgttcccgcc
     4381 gccacgccca caccgacggg ctgacataat gcacggtcgg aatccccgac cgtttcagct
     4441 tttccgccac acccaaatta aaatcgggcg catcgatacc gacaaagaca tcaggtttca
     4501 acgacagcaa atcccgtacc agccccctgc gtatccgtaa aatttccggc agccgcctga
     4561 ccacttcgac aaagccgcgc accgccagcc gctcctgatc ataaaggctc tcgaaacctt
     4621 ccgccttcat cagttcgccg ccgataccga caaaacgcgc ctgcggacaa cgcttgcgga
     4681 tggcgcgtat caggtgcgcc cccaataggt cgcccgacgc ttcgccgaca ctgacggcaa
     4741 tcaaagggct ttttttatca gccatattcg tctgtcccca catactttca cgtcccgatg
     4801 ccgtctgaag gcttcagacg gcatcggggt tcacattttc gtcccgtttt ccaacatcac
     4861 cgcaaactgc gccaagtccg gcggcagctc cgccttcaac accagcggct cgcccgtgag
     4921 cggatggttc aagtgcagct cggacgcgtg caaaaacatc cgcttcaaac ccaacttctg
     4981 caaacgacgg ttcgcctgat aatcgccgta gcgttcgtcg cccgcaatcg gacagccttg
     5041 agattgcagg tggacgcgga tttggtgcgt acgacccgtt ttcagcgttg cccgcaccaa
     5101 agtcaggtgc gacagcccga caccgtgcaa aatgccgtct gaaaaacggc ttaacacacg
     5161 gaacaccgtg tgcgccgact gcccgtcctc gctgacgcgt accatctttt cgccttgtgc
     5221 gccggtatat ttgaacaggg gcagtttgac atggaaattg tcgtccggca gtttgcccac
     5281 ccccagcgca aggtagattt ttttggggtg gtcgttacgg atggcttcgt gaagtttgac
     5341 gagcgcgctg cgtttcttcg ccaccatcaa caagccgctc gtatccttgt ccaaacgatg
     5401 aaccaactcc aaatacttcg cctccggacg ggcgcggcgc aactgttcga taacgccgaa
     5461 actcacgccg ctgccgccgt ggacggcaac gccggacggt ttgtcgatga ccaaaagcgc
     5521 atcgtcttcg taaacaacgt caaacgcacg cgccggtacg gcggcacgcc tttcagacgg
     5581 catttccttc tccgccacgc gcacaggcgg aatccgcacc gtatccccct ccgcaatacg
     5641 gctgtcgggt ttgcagcgtt tcttgttcaa ccgcacctcg ccggcgcgga taatgcggtg
     5701 gatatggctc ttgggaacac ccttgaggat ttttatcaga tagttatcaa ggcgttgacc
     5761 cgcctcatgt tcggcaaccc ctatcaagct gaccgaatct ttgcttattt cgtgcgtttt
     5821 catctataat ccgaacatcc gtttcagcaa aaagcgcgcc cgccgcgccc ccctgaaacg
     5881 gtttacttta aaacggaatt ttatattaaa aacgcactcc gcaaagcatt ttccctgcgc
     5941 ctgttcccag ccggcaggcg cagaacgtaa tcaagtttga attgattttg ccgtttcggc
     6001 gcggaagtta agacggcagc cgggccccaa gtcccaaacg caaaccgccc aaaacacagg
     6061 catcacgata acaagaagaa gccggccccc atttttccga ccgcagcgtt cggaacgcgg
     6121 cagacaaacc gccgcatcga tctttatagc cttctgtatc cgacccttcc gcacacggtt
     6181 ctccggaacg tgcatccctg cggattttca actcaaaaac tgattaccgg ttttatccga
     6241 aaacatcgga aaacccagcc ttacggaatg ccgaaccgga ccggcgcgcc cgaatgccgc
     6301 ccgtccacga ggtgatcatg aaaagaatgt tattcaacgc aacgcaggcc gaagagctgc
     6361 gcgttgccat cgtcgacgga caaaacctgc tggatttgga catcgaaacg ctgggcaaag
     6421 aacagcgcaa aggcaatatc tacaaaggca tcattacccg catcgagccg tcgctggaag
     6481 cgtgtttcgt ggattacgga accgaccgcc acggtttctt gccgtttaaa gaagtctccc
     6541 gctcatactt ccaagactac gaaggcggac gcgcgcgcat ccaagacgtg ctcaaagaag
     6601 gcatggaagt catcgtccaa gtcgaaaaag acgagcgcgg caacaaaggc gcggcgctga
     6661 ccaccttcat cagcctcgcc ggacgctatc tggtattgat gccgaacaac ccgcgcggcg
     6721 gcggcgtatc ccgccgtatc gaaggcgaag aacgtcaaga actcaaagcc gctatggcgc
     6781 aactcgacat tccgaacggc atgagcatca tcgcccgtac cgccggcatc ggccgcagcg
     6841 cggaagagtt ggaatgggat ttgaactatc tcaaacaact ctggcaggcg attgaagaag
     6901 caggaaaagc gcatcacgac ccttacctgc tctttatgga aagctcgctg ctcatccgcg
     6961 ccatccgcga ctatttccgc cccgacatcg gcgaaattct ggtggacaat caagaagttt
     7021 acgaccaagt tgccgagttc atgagctacg tcatgccggg caatataggc cgtctgaaac
     7081 tctacgaaga ccacacgccg ctgttttccc gcttccaaat cgaacaccaa atcgaaagcg
     7141 cgttttctcg cagcgtcagc ctgccttccg gcggcgcaat cgtcatcgac cacaccgaag
     7201 ccctcgtctc catcgatgtc aactccgcac gcgccactcg cggcgcagac attgaagaca
     7261 ccgcgttcaa aaccaatatg gaagccgccg aagaagtcgc gcgccaaatg cgcctacgag
     7321 acttgggcgg tttggtcgtc atcgacttca tcgacatgga aaaccccaaa caccagcgcg
     7381 atgtggaaaa cgtcctgcgc gacgcgctca aaaaagaccg cgcccgcgtg cagatgggca
     7441 aactctcccg cttcggcctt ttggaattga gccgccaacg cctgaaacca gctttgggcg
     7501 aaagcagcca cgtcgcctgt ccgcgctgcg ccggcaccgg cgtgattcgg ggcatcgaat
     7561 ccaccgccct gcacgttttg cgtatggttc aagaagaagc aatgaaagac aacaccggcg
     7621 aagtgcgcgc ccaagtgccc gtcgatgtcg ccaccttcct gttaaacgaa aaacgcgccg
     7681 agctgtttgc gatggaagag cgtttggatg tcaacgtcgt cctgattccg aacatccacc
     7741 tcgaaaaccc gcactacgaa atcaaccgca tccgcaccga cgacgtagaa gaagacggcg
     7801 aaccgagcta caagcgcgtc gccgagccgg aagaagacga atccgccaaa cctttcggcg
     7861 gcgaaaaagc caaagccgcc cgtcccgaac ccgccgtcaa aggcgtgcgc cacaccagcc
     7921 ccgccccgac tgccgccccc gagaaaaaaa cctcctggtg ggacagcttc aaagcatggc
     7981 tcaaacgcat tttcggcggc agcgaaaccc aagccgcgcc cgctgccgaa acctccgaaa
     8041 aacgcagcac ggcaaaccgc agcggcagcc gcgccaacaa ccgccgccaa aacccgcgcc
     8101 gcagcaaacg cgaaggcagc aaagtagaag tccgcgaagt ggcaggcaaa actgccggac
     8161 aggaagcgcg tgccgacaaa gccgaaacgc gcaacaacgg caaccgccgc cgcaacgaac
     8221 gcggcgaccg tgccgccgaa cgcgccaacg aagcggaaat ccaagaccgc aacatacagc
     8281 ctgccgcaac cgttgcagat gccgcaccgt ccgaaaccga agtgcaaacc ggcaaacgcc
     8341 gccgcaacgg cagccgcaac gaacgcggcc aaaccgcgct ggaaaccgcc gccgttgccg
     8401 aaacagccgt tcagacagcg gaaaacacgc cgtccgaacc gcataccgca gaagacaaag
     8461 gcagcaagcc caaatccgaa cgcaaccgcc gcgaacgcga cagccgcgac gccaaagaac
     8521 gccgcgagcg caacaatcag cgcgaccgcc gtcaaaacgg caaaaaacgc aatatcccgt
     8581 ctgccgccaa aatcgagcag tacctgaata ttcacgacac tgccgacaaa gtccgttccg
     8641 ccgccgcaca cgttttcgga gaaaccgacg caaacgcgcc gattaccgtc agcattgccg
     8701 atccgctcat cgccacaccc gttcagacgg catcttccgc cgtttcaaac ggcgacgcac
     8761 tggtttatga tgcggcagaa aaaatccgcc gtgccgccgc catcctgccc gaaggcgcga
     8821 caccgaaagc ctcagcacag gaaatgccgt ctgaaaccgc aacctttacg gctgcggcgg
     8881 aacaggcacg ggaaaccgca caaaccggcg gactcgtcct gatcgaaacc gaccctgccg
     8941 cattgaaagc atgggcggca caacccgaag tccaaaccgg acgcggtttg cgccgttccg
     9001 aacagcccaa gccgtctgaa gccgcaaccg tccctgccga agaaatgatc caagtcgaaa
     9061 cccggcaagg ctgaaccgac ggcggcaaaa agaggttctg ttccgcagaa cctctttttt
     9121 atatagattc ggatacctgc aatgccgtct gaaacttcgc cattcccgtg attaccgaaa
     9181 cattccgcca ttcccatgat tcccgcaaca ttccgtcatt cccgtgaaaa cgggaatcta
     9241 gaacctcaaa ctttcagata acctttgaat attgccgccg cccgaaggcc tggattcccg
     9301 cctgcgcggg aatgacggtt tagaagttgc ccgaaacctc aaaaaaaaaa ccgaaaccga
     9361 acaagccgga ttcccgcaac attccgtcat tcccgtgaaa acgggaatct agaacctcaa
     9421 actttcggat aatctttgaa tattgccgtc gcccaaaggc ctggattccc gcctgcgcgg
     9481 gaatgacggc ggagggtgga cgatgccgtc tgaaactgcg ccattcccat gattaccgca
     9541 acctttcgtc attcccgcca cctttcgtca ttcccgtgaa aacgggaatc tagaacctca
     9601 aactttcaga taatctttga atattgccgc cgcccaaagg tctggattcc cgcaacattc
     9661 cgtcattccc gtgaaaacgg gaatctagaa cctctaaact ttcagataat ctttgaatat
     9721 tgccgtcgcc caaaggcctg gattcccgcc tgcgcgggaa tgacggttta gaagttgccc
     9781 gaaacctcaa aaaaaaccga aaccgaacaa gccggattcc cgcaaccttt cgtcattccc
     9841 gtgaaaacgg gaatctagaa cctcaaactt tcagataatc tttgaatatt gccgtcgccc
     9901 gaaggtctgg attcccgcct gcgcgggaat gacgatttgg aaattacccg aaacccaaaa
     9961 acaactgaaa ccgaacagac cggattcccg cctgcgcgga aatgacgggt cttttatcat
    10021 ctttaaaggc tgccgcgcgc catctcgacg gcggtctcca cggcagttat caggctgccg
    10081 gaatccgccc tgccggttgc cgccaaatcg agcgcggtgc cgtgatcgac ggaggtgcgg
    10141 ataaagggca gccccagcgt gatgttcacg ccctgtccga agctgtggta tttcaacacg
    10201 ggcagccctt ggtcgtggta catcgccaat acggcatccg caccttcgag cataaacggt
    10261 tggaacaagg tatccgccgg atacgggccg gcgaggttta tcccttcgcg gcgcaggttt
    10321 tccaatgcgg ggataatggt gtcggtttct tcgtgtccga ggtgtccgct ctcgccggcg
    10381 tgggggttga gtccggcgac taggattttg ggatttttga tgccgaattt gtgttttaag
    10441 tcgtgatgca aaatgcgtgc gacgctttca atcagcggtt gcgtgatggc ggcggcaacg
    10501 tctttcagcg gcaggtgggt cgttacgagg gcgacgcgca ggcctttgcc ggcaagcatc
    10561 atcacaacct gccccgtgcc gcttttttcc gctagatatt cggtgtgccc gctgaaaaaa
    10621 cctgtgcttg cgcgcgcgtc gttgatgatg cctttgtgca gcggcgcggt aacgatgccg
    10681 tcgaaaatgc cgtctgaaat gcctgcgagc gcggtgtcca aaagttgcag cacataggcg
    10741 gcgttggcgg gattgagccg ccctgccgca accgcttcgg cggtcggaat gtgcagcact
    10801 tccagctcgc cttctccggg tgcgccgcct gattccggat cgaagtcgcg caagacgacg
    10861 cttttgccca aggcttcggc gcgcgcgcgc aataggtttt tgtcgcccaa taccgcgcag
    10921 cggcagggca ggcgtgcaaa cgccaagtcc aaacaaatat cggggccgat gccggcaggc
    10981 tcgccggaag taacggcaaa aacaggctgt ttcatcgtgt ttgctccaaa caaaatgcga
    11041 ttctaacgcc gcagccgcgc ggcgatgtaa atttttctga ttttgttgac aatctgctag
    11101 aatgggcgtt tacaaaattt aaaccctgct tgcataccgc caatatgtgc gagtttcaac
    11161 tttaaggaag cgatatgaac gagaacttta ccgaatggct gcacggctgg gtcggcgcca
    11221 tcaacgatcc gatgtggtca tacttggttt atatgctttt gggtacgggg cttttcttca
    11281 ccgtaaccac gggctttgtc caattccgcc tgttcgggcg cagcatcaaa gaaatgctcg
    11341 gcggccgcaa acagggggac gaccctcacg gcatcacgcc gtttcaggca tttgtaaccg
    11401 gccttgccag ccgcgtgggc gtgggcaata tcgcgggcgt ggccatcgcc atcaaagtcg
    11461 gcggaccggg cgcggtgttt tggatgtggg taaccgcctt aatcggtatg agttcggcgt
    11521 ttgtcgaatc ttcgctggcg cagctcttta aagtccgcga ctacgacaac caccatttcc
    11581 ggggcggccc tgcctactac atcactcaag ggctggggca gaaatggctg ggcgtgttgt
    11641 tcgccctgag cctgattttc tgtttcggct ttgtgtttga agcggttcag accaatacca
    11701 ttgccgatac cgtcaaagcg gcgtggggtt gggagcctca ttatgtcggc gtcgccctgg
    11761 tgattttaac cgcgccgatt atcttcggcg gcatcaggcg catatctaaa gcggcggaaa
    11821 tcgtcgtccc cctgatggcg gttttgtacc tctttatcgc gcttttcatc attttgacca
    11881 atattccgat gattccggac gtgttcggtc agattttttc gggcgcgttc aaattcgacg
    11941 cggcagcagg cggcttactc ggcggtctga tttcgcaaac gatgatgatg ggcatcaaac
    12001 gcggcctgta ttccaacgag gcgggtatgg gttccgcgcc gaacgccgcc gccgccgccg
    12061 aagtgaaaca ccctgtttcg caaggtatga ttcaaatgct gggcgtgttt gtcgatacca
    12121 tcatcgtttg ttcttgcacc gccttcatca tcttgattta ccaacagcct tacggcgatt
    12181 tgagcggtgc ggcgctgacg caggcggcga ttgtcagcca agtggggcaa tggggcgcgg
    12241 gcttcctcgc cgtcatcctg tttatgtttg ccttttccac cgttatcggc aactatgcct
    12301 atgccgagtc caacgtccaa ttcatcaaaa gccattggct gattaccgcc gttttccgta
    12361 tgctggtttt ggcgtgggtc tatttcggcg cggttgccaa tgtgcctttg gtctgggata
    12421 tggcggatat ggcgatgggc attatggcgt ggatcaacct tgtcgccatc ctgctgctct
    12481 cgcccttggc gtttatgctg ctgcgcgatt acaccgccaa gctgaaaatg ggcaaagacc
    12541 ccgagttcaa actttccgaa catccgggcc tgaaacgccg tatcaaatcc gacgtttggt
    12601 aaatcccgcc cttaccagag ccgcttcccc gcgaagcggc ttttcccttt ccgcacactg
    12661 taaaaacagg gcgaacaagc gtacaatccc aaccctttac ttttgaatcc atttcgtttt
    12721 tcagacggca tattgaatat agtggtttaa caaaaatcgg gacaaggcgg cgaagccgca
    12781 gacagtacag atagtacaga accgattcac ttggtacttc agcaccttag agaatcgttc
    12841 tctttgagct aaggcgaggc aacgctgtac tggtttttgt taatccacta taaatgccgt
    12901 ctgaaacacc gtcaggcaat acacacatga cccacatgat ttaccccaaa acctacgacg
    12961 ttatcgtcgt cggcggcgga cacgccggca cagaagccgc gctcgccgcc gcccgtatgg
    13021 gcgcgcagac gcttttgctc acacacaata ttgaaacgct cggacaaatg tcgtgcaacc
    13081 cttccatcgg cggcatcggc aaagggcatt tggtgcgcga actcgacgcg ctcggcggcg
    13141 cgatggcgtt ggcaaccgac aaatccggca tccagttccg ccgtctgaac gccagcaaag
    13201 gcgcggcagt acgggccacg cgcgcgcagg cggatcgtat cctatataaa gccgccatcc
    13261 gcgaaatgtt ggaaaaccaa gaaaacctcg accttttcca acaagccgtc gaagacgtaa
    13321 cgctcgatgg cgaccgcatt tcaggcgtaa tcaccgcgat gggcgtggag tttaaagcac
    13381 tcgccgtggt gttgaccgca ggcacgtttt tgtccggcaa aatccacatc ggtttggaaa
    13441 actacgaggg cggacgcgcc ggcgaccccg ccgccaaatc gctgggcgga cgtttgcgcg
    13501 aattgaacct gccgcaaggc cgtctgaaaa ccggcacgcc gccgcgcatc gacggacgca
    13561 cgattgactt ctcccaactg accgaacagc ccggcgacac gcccgttccc gtcatgtccg
    13621 tacgcggcaa cgccgaaatg cacccgcgcc aagtgtcctg ctggattacg cataccaaca
    13681 cgcaaaccca cgacatcatc cgctcaggct tcgaccgcag cccgatgttt accggcaaaa
    13741 tcgaaggcgt gggcccgcgt tattgtccgt ctatcgaaga caaaatcaac cgcttcgccg
    13801 acaaagacag ccaccagatt ttcctagaac ccgaaggcct gactacgcac gaatactacc
    13861 ccaacggtat ctccaccagc ctgccgttcg acatccaaat cgcgctcgtc cgcagtatga
    13921 aaggtttgga aaacgcccat atcctgcgcc ccggctacgc catcgaatac gactacttcg
    13981 atccgcgcaa cctcaaagca agcctcgaaa ccaaaaccat tgccggattg tttttcgccg
    14041 ggcaaatcaa cggtacgaca ggttatgaag aagccgccgc gcaaggactg ctggcaggcg
    14101 cgaacgccgt gcaatacgtc cgcgaacaag acccgctcct gctgcgccgc gaacaagcct
    14161 atctcggcgt attggtggac gacctcatca ccaaaggcgt gaacgaaccc taccgaatgt
    14221 tcaccagccg tgccgaatac cgcctgcaac tcagggaaga caacgccgat atgcgcctga
    14281 ccgaagacgg ctacaaaatc ggcttggtgg gtgaagcgca atggcgcatg ttcaacgaaa
    14341 aacgcgaagc catcgaacgc gaaatccaac gtttgaaaac aacgtggtac acgccgcaaa
    14401 aactcgccga agacgaacaa atccgcgtat tcgggcaaaa gctcagccgc gaagccaacc
    14461 tgcacgacct cctgcgccgc ccgaacctcg actacgccgc gctgatgacg ctcgaaggcg
    14521 cgatgccgtc tgaacgcctc tccgccgaag tggtcgaaca agtcgaaatc caagtcaaat
    14581 accaaggcta tatcgaccgc caaaacgaag aaatcgacag ccgccgcgac atcgaaacct
    14641 taaagctgcc cgaggatatc gattacagca aagttaaagg tttgtcggca gaagtgcagc
    14701 aaaagctcaa ccagcacaaa cccgaaaccg tcgggcaggc aagccgcatt tccggcgtaa
    14761 cccctgcggc agtggcattg ctgatggtgc atttgaagcg cgggtttaaa ga
//
LOCUS       DAXHTR010000042        14751 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.042, whole genome shotgun sequence.
ACCESSION   DAXHTR010000042 DAXHTR010000000
VERSION     DAXHTR010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14751
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.042"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            52..1005
                     /locus_tag="ACFNML_000857"
     CDS             52..1005
                     /locus_tag="ACFNML_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFC6392266.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSIGGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHGVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="ACFNML_000858"
     CDS             complement(1116..1439)
                     /locus_tag="ACFNML_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392267.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGSN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="ACFNML_000859"
     CDS             complement(1612..2973)
                     /locus_tag="ACFNML_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFC6392268.1"
                     /translation="MLKKWLNKMLPSSRSSKEAESKTVIPAERHNIRAEMLSFAAENV
                     IRRLKEAGFQAYVVGGAIRDLLLGIEPKDFDVATDAMPEQVHKLFRRSRIVGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSGKLGFELSEETTAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLAALMWPELERHWKSNLQQGLKPAPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
     gene            complement(3328..5028)
                     /gene="recJ"
                     /locus_tag="ACFNML_000860"
     CDS             complement(3328..5028)
                     /gene="recJ"
                     /locus_tag="ACFNML_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFC6392269.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAAGRLADAVERKEKILIVADYDADGATACSVGMSGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAEQGVDLLITVDNGIASIAGVARAQALGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFPSKSLAGVGVIFYVLMALRAELRRRNYFSDGIKEP
                     NLGELLDLVALGTVADVVPLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDAEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQTTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKAWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAA
                     "
     gene            complement(5097..5813)
                     /locus_tag="ACFNML_000861"
     CDS             complement(5097..5813)
                     /locus_tag="ACFNML_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392270.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDRPADIAKLKQLEFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPVDIYYTVTEKHTDKSKLFGNILYTPPLLILDAA
                     GAVLALPAAALGAVVDAARK"
     gene            complement(5860..6366)
                     /gene="yjgA"
                     /locus_tag="ACFNML_000862"
     CDS             complement(5860..6366)
                     /gene="yjgA"
                     /locus_tag="ACFNML_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFC6392271.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MENGDAEI"
     gene            6520..7851
                     /gene="pmbA"
                     /locus_tag="ACFNML_000863"
     CDS             6520..7851
                     /gene="pmbA"
                     /locus_tag="ACFNML_000863"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFC6392272.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADACLMAKHIGDPDLYHEWDLDTEAAVGLAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            7863..8054
                     /locus_tag="ACFNML_000864"
     CDS             7863..8054
                     /locus_tag="ACFNML_000864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392273.1"
                     /translation="MPSEDEGSTFCPVPARLPFYAAPPFADNIAHFFISTDFPLSVKI
                     LSHLSTTGYTQVQDKACGD"
     gene            complement(8148..8351)
                     /locus_tag="ACFNML_000865"
     CDS             complement(8148..8351)
                     /locus_tag="ACFNML_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFC6392274.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            8594..8896
                     /gene="clpS"
                     /locus_tag="ACFNML_000866"
     CDS             8594..8896
                     /gene="clpS"
                     /locus_tag="ACFNML_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFC6392275.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            8898..11189
                     /gene="clpA"
                     /locus_tag="ACFNML_000867"
     CDS             8898..11189
                     /gene="clpA"
                     /locus_tag="ACFNML_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFC6392276.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDEDEDDGNYSSDGMD
                     DDNENRTKPGKNPLSAYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLV
                     GEAGVGKTALAEGLAHQIVNGGIPDALKDAEVYALDMGSLLAGTKYRGDFEARVKSVL
                     KQLEKIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRT
                     IFDKDHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSAR
                     YINERFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVARIPEKTVSHDD
                     KQVLQFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTE
                     AAKQLAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVL
                     LLDEIEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGF
                     TAKRERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKK
                     VEAEFTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLSDGGFVRIDW
                     DAAKEEAVLKFKKSKVKIKTASA"
     gene            complement(11229..14327)
                     /locus_tag="ACFNML_000868"
     CDS             complement(11229..14327)
                     /locus_tag="ACFNML_000868"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HFC6392277.1"
                     /translation="MNLETKPIAETPNFIVLDQYEKIEQSGSYQSENRLEAELIADLQ
                     NQGYEYRKDLNSQSRLLENLRAQLQRLNDVAFSDGEWARFLTEYLDRPSENITDKTRK
                     IHDDHIYDFAFDDGRLKNIYLLDKKNLARNHVQLINQFEQTGTHANRYDVTVLVNGLP
                     LVQIELKKRGVAVREAFNQVHRYSKESFNSENSLFKFLQIFVISNGTDTRYFANTTKR
                     DKNSFDFTMNWARSDNHPIKDLKDFTATFLQKSVLLSVLLHYSVFDANDTLLIMRPYQ
                     IAAAERILWKINSSAQAKNWSKPESGGYVWHTTGSGKTLTSFKAARLATESAFIDKVF
                     FVVDRKDLDYQTMKEYQRFSTDSVNGSESTAGLKRNLEKDDNKIIVTTIQKLNNLMKS
                     EDNLPVYHQRVVFIFDECHRSQFGEAQKNLKKKFKKFCQFGFTGTPIFPENALGAETT
                     AGVFGRELHSYVITDAIRDEKVLKFKVDYNDVRPQFKAVEAEQDEKKLSAAENRQALL
                     HPERIREITQYILNQFRQKTHRLNAGGKGFNAMFAVSSVDAAKCYYEAFKTQQAGSLH
                     PLKVATIFSFAANEEQNAVGEIVDETFEPEAMDSSAKEFLQAAINDYNACFKTNFGTD
                     SKAFQNYYRDLAKRVKNQEIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSR
                     TNRIYDATKTFGNIVCFRDLEQATIDAITLFGDKNTKNVVLEKSYEEYMNGYIDSQTG
                     EARRGYLDVAKELRERFPDPDEIETEKDKKDFAKLFGEYLRAENVLQNYDEFAALREL
                     QNVDAADEDAMKAFQEKYYLSDEDVQEMRQVPMPSERAVQDYRSAYNDIRDWLSRQKA
                     GEQKEQSKIDWDDVVFEVDLLKSQEINLDYILQLVFEHHKKIKGKAELVEEIRRIIRA
                     SIGHRAKEGLIVDFINDTDLDKVPDVPAILETFYTYAQEVMRHEAAGLIAAEDLNETA
                     AKRYLTGSLKRGYASENGTELTETLPKMSPLNPQYLTKKQSVFQKIAAFVEKFAGIGT
                     DI"
     gene            complement(14406..>14751)
                     /locus_tag="ACFNML_000869"
     CDS             complement(14406..>14751)
                     /locus_tag="ACFNML_000869"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6392278.1"
                     /translation="VNYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLINT
                     SSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDILTHSISEGLPHEIALRRKQYEY
                     YREQLLAFPKAT"
BASE COUNT         3417 a         4169 c         3675 g         3490 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgt
      361 accaaacgcg gcagcatcgg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgcc
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggt tttctgcccg gtgacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgtc
      661 accaagctga ttgaaaaagg cctgattgaa atcgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 ggcgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcgggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gctgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg caggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgtttcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 tagactttat ttacggggat gaatcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca tcccatgtgt tgaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggacgt gcgcctttgc ggttttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gcacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcgcgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagagccgc caatacaaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaaca cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcatga tttcgtcaaa
     2281 cagcctcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggtggt
     2341 ttcttccgac agctcaaagc ccaatttgcc cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc gccaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tccggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcctt
     2581 cataatcctg ccgtgcgcgt tctgatgaac tttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac gatttgaaaa cgcctgccga cgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg catggcatcg gttgcgacat cgaaatcctt
     2761 gggttcgatg ccgagcagca ggtccctgat cgcgccgccg accacataag cctgaaaccc
     2821 tgcctctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgaccgtttt actttccgct tctttactgc tccgactgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctgacccgaa gagcggcatc
     3001 tccacgggca accgtgttca gacggcatcg ggcgcaggtt tgcaagatgc cgtaaccata
     3061 aacaaaaggc gtagaaacta cgccgtccga atcacgccgc cctcgcggcg gcaacgcgtc
     3121 attataacag attgccctcc aaaaaaatca gaccctgctt ttgtggcaga gtctgaaatt
     3181 tgatgcacct tatatattgc gtccctaaga agggatgatt aacaaaaatt aacgcccttt
     3241 actttctttt ccgattggga acattcccgt cgtacaaaca ggcaaatgaa ggaacagaaa
     3301 tcacattcaa acggcgttcc gccgcctcta cgccgcttcc cagtagtcga tataaagctg
     3361 caattcgagg ttgttgcgcc attcgttggc aacggggcgg tacaccgtgc ggatgtattc
     3421 gggaatgtct tcgctgcacc gccagaacat tgcttcaaat tcgcagccgt ctttttgcag
     3481 ccacgctttt ttgtgtttgc cttccgcgcc caaaggctgc tggcggacga cgtggaactc
     3541 gtcggtaaaa ctcggcggcg cgaagccctg cccccaaacg tgtttagcaa ggttttgcgc
     3601 ctgttccagc gtaatgtcgc gtgcaggcag gctgccgtcg gtgatgaagg tttgcgacaa
     3661 atcgtcttcg cacaccattt cgcgcacggc ttcttcaaag gccgtctgaa acgcgggaat
     3721 gttgtgttcg agtatgctca agcccgccgc catcgcgtgt ccgccgaatt tcaaaatcaa
     3781 atcgggatgg cgtttggaca ccaagtccaa agcatcgcgc aagtgaagat tgggaatgga
     3841 acgccccgaa ccgcgcactt caccgttgtc ggcaggcgca aacacgatgg tcggacgata
     3901 aaaacggtct ttgaggcggc tggcaacgat gccgaccacg ccttgatgga agtcgtcgcg
     3961 atacgccacc aaagtcgtct gacctgaagg cagggtttca ggaaaagcat tcagcgcgtc
     4021 ttgcagcata gactgctcga tttcgcgacg ttcgatattg aggttgttta actgcgccgc
     4081 cagttcctgc gcctcggcat catctcgcgc caacagacag gcaataccga ccgacatatc
     4141 gtccagccgt ccggcggcgt tgatgcgcgg ccccaacgca aaacccatat caaacggctg
     4201 cgccttgcgc caatcgcgcc gcgccacttc aaacaaggcg cggataccgg ggcgcatttt
     4261 gccggagcgc atccgtttca aaccttgcga cacgaggatg cggttgttgt ggtcgagagg
     4321 gacgacatcg gcaaccgtgc cgagtgcgac caaatccaaa agttcgccca aattcggctc
     4381 tttgatgccg tctgaaaaat aattgcggcg gcgcaattcg gcacgcaacg ccatcaatac
     4441 ataaaaaatc acgcccacgc ccgccaagct tttgcttgga aaaccgcagc ctttttggtt
     4501 cggattgacg atgatgcagt cgggcacggt ctcggcaggc agatggtggt cggtaacgat
     4561 gacatccaaa cccagagcct gcgccctcgc cacacctgcg atgctggcga taccgttatc
     4621 aaccgtaatc agcaaatcca caccttgctc tgccgcgatt tcagccagct cgggcgttaa
     4681 gccgtagccg tgctcaaagc ggttgggcac aaggaaatcc actttcgccc ccatcgccgc
     4741 caaaccgctc ataccgacgg aacacgccgt cgcgccgtcg gcatcatagt cggcgacaat
     4801 caggattttt tccttgcgtt caaccgcatc cgccaaacgg ccggcggcgg cttcgcagtt
     4861 tgtcagcgtt tgataaggca ggagggaagc gagtttgtcg tccaattcgg caggactttg
     4921 cacaccgcgc gaggcacaaa gccgggcgat taaaggatcg gcaccggcgg tgagcaaatg
     4981 attaaaaacg tcggtattga cggatcgggt ttggattttg actgacatga cgaagttttt
     5041 tcaagatatg atgaggctga aatgccgtct gaaagctttt cagacggcat tgcttttcat
     5101 ttgcgggcgg catccacgac cgcgcccaga gccgccgcag gcaaagccag taccgcaccc
     5161 gccgcatcca gtatcaacaa ggggggcgta tataagatat ttccaaacag cttggatttg
     5221 tcggtatgtt tttcagtaac cgtgtaataa atatcgacag gcacactctg ttcaaaatgg
     5281 taatcggcgt tcagtttttg cggtgtggcg tagtatttgc ctttggcgga tacgcagcgc
     5341 gtgtaaatag tccgattgtc aagttcgacc gcttcaaact caagctgttt cagcttggcg
     5401 atgtcagcag gcctgtcggt atcgtagcgc aggcaaaggc cttcggtact gaaattctgg
     5461 ctgccaggcg attcgagttt gaccggcagg gcttggtggc gagcatagct cggggtatcc
     5521 tcaactattt ggaagggttt gtccaacccg gccttcaaaa tgcccgtcag cttcgccgaa
     5581 tcttcgggat tgacgacgaa ccagtatttc ccgcccatca tcaccagact gcccttttcc
     5641 aattgggcat tgtctttggc aaccacaccg aaggcgcgga tttgatcctt gtcaacgtgt
     5701 ttataggcgg tcgtttggct gaacgggtcg ttcatgcccc acatcattgc cgtacagccg
     5761 ttcaacatca aggctgccgc caaaacggcg gcggtcgttt tacggaacaa cattttatcc
     5821 tccaatctaa ataacggcgg atgtcgtctg aaaatatgcc taaatttccg cgtccccgtt
     5881 ttccataacg gttttcaact cttgaaacag ggcgcggaaa tttttcggcg gtttgttctg
     5941 ttcctgttct tttttggtat tgcggatgag cgtcctcagc ttgcccgcat cggcgtttgg
     6001 aaaatcggac ataaactgcg tcagcgcgcc gtcgtccgcc agcagctcca tccgcgtccg
     6061 ctccacacgt tgcaaaaagg cgttgtgcgc cgcatcgtcg ccgcgcagct tggcaagaaa
     6121 tgcctcgatg ggcgcgggat cggtatcgcg catcagtctg ccgataaatt gcgcctgccg
     6181 cttgagcgcg ccgttggatg tgattttttt ataggtaacg atggcttcgt acaaatcttc
     6241 gtccaagccg atttttttca acgtgtcgct tgagagcttg gtcagctcca tacccaaatc
     6301 ctgcaaaccg ttcatctgct ttttcatttg ggttttgctg acccattcgt cttcttgttc
     6361 aaacatcttc attctcaaat tttatccgac gggcgcattt taacagaatg attccgcgtt
     6421 cagacggcat tttgcaccgt ccgccctgca aacggacgct tttgcccctg ccgcccgaaa
     6481 cggttaaaat gccgcaacgc tccaccaaga aagatttcta tgctgttcaa ccacaccgct
     6541 tccgaacttc tcgacctctg ccgccgcacg ctcgacttgg caaaagcagc gggcgcgacc
     6601 gccgccgaag ccgatttcag cgaatccatc ggacaaagcg tcagcgtgcg gctgggcgaa
     6661 atcgaacaaa tcgagttcca gcaggacaag tcgctggaca ttaccgttta cgtcggcaaa
     6721 cgcaaaggcc gcgctggcac cgccgatttc tccgaacaag ccctgcgcga caccgtccgc
     6781 gccgccgccg acatcgcccg ccacaccgcc gaagacggtt gcgccggact tgccgatgcc
     6841 tgtctgatgg caaaacacat cggcgacccc gacctttacc acgaatggga tttggatacg
     6901 gaagccgccg tcggcttggc aaaacaatgc gaacaagccg ccctgaacga ggatgagcgc
     6961 atcgaaaact ccgaaggcgc ggcggtgcaa accggccatt accaatacgt ttacggcaac
     7021 acccacggtt ttgccgcaca ccggcaaggc acgcaccaca gcatttcctg cagcgtcgtt
     7081 gccgccgacg aaaacggcat gcagcgcgac tactggtacg attccgcctg ccgccatccg
     7141 gatatggaca gccccgaaac catcggtcaa accgccgccc gccgcacttt gcgccgactc
     7201 ggcagccgca gcatcccgac aggcagctac cccgtcctct tcgataccac cgtttcgggc
     7261 ggtcttatcg gacacctcgt cggcgcactc tccggcggtg cgctctaccg ccaaagcagt
     7321 ttcctgatcg acagcatcgg caaaaaagtc ctgcccgatt tcctcaacct gcgcgaagaa
     7381 ccgcacatcc cccgctcttt ccgcagcagc tattttgatg cggaaggcgt tgccaccgcg
     7441 ccgcgcttcg tgattcaaaa cggcattgtc gaaggctatt tcctcagcag ttacagcgcg
     7501 cgcaaactcg gtatgcagac cacgggcaac gccggcggcg cgcacaacct gtatttgaac
     7561 catacgcacg aaacgcaatc cgacctgctg aaagaaatgg ggacgggatt gttggtaacc
     7621 gaactgatgg gacagggcgc gaacaccatt accggtgact actcgcgcgg cgcggcgggt
     7681 ttttgggtgg aaaacggcgt gattgcctac cccgtccacg agattaccgt agccggacgc
     7741 ttgcaggata tgtaccgcga cattgtcggc gtggcggatg acgctttgcg gcgttcgtcc
     7801 aacaaaatcg gctcgattct gattgcgggg atgacggttg ccggaagctg aacgccctgc
     7861 cgatgccgtc tgaagacgaa ggcagcacgt tttgccccgt gcctgcccgt ttgccgtttt
     7921 atgccgcacc gccttttgcc gacaacattg cacatttttt catcagcact gattttccct
     7981 tatccgtcaa aatactaagc cacttgtcca caacagggta cacacaagtt caggataagg
     8041 cgtgcgggga ttagggtttt tccgccgccg gcaaaacggc agacaaaaac ggcgggacat
     8101 tgcctgtccc gccgttttca aatatacatt tgaccgtaca tcaggaatta agcagcctga
     8161 atgttggcgg cctgtttgcc tttagggccg gtggttacgt cgaaagagac gcgttggcct
     8221 tctttcaggg ttttgaaacc ttccatattg attgctgaga agtgagcgaa caaatcttcg
     8281 ccgccttcat caggagtgat gaaaccaaaa cctttagcgt cgttaaacca ttttacgata
     8341 ccggttgcca ttagaaactt cctatactca aaaattaaca aaatcagcaa aacaaggcat
     8401 acggcaaacc gagccgtcta gcgttccttc cagcttgata tactcggcgg atgcccaatc
     8461 ctgcttaacc gtctttttac atttgttaag acaaaacgtc aagctatgtt ttcaaaaaag
     8521 tgggaaaata ggcaaaagta ccgaaaatac aacaaaaatg ccgtctgaac gcggctttca
     8581 aatgggacac actatgaccg cgcaacacca atccgacacg ctcttgcacc gtctgaacac
     8641 cctgccgccg aaacgttacg gcgttttcct attgaacgac gattacacca cgatggaatt
     8701 tgtcgtcgaa atcctgaccg aaatctttat gctcggacaa gaacaggcgg tagcggtgat
     8761 gctcttggtt catcacgaag gcaaaggcct gtgcggcact tacacgcgcg atattgccca
     8821 aaccaaacaa caacaagtca tgcagcgggc aaaagccgaa gggcatccgc tgcaatgtat
     8881 tgtcgaggag atttaatatg cttgcacccg aattggaaca gattttgcag cagctttacc
     8941 gcgaggcgcg taaggctcat tatgaattta tcagcctcga gcatctgctt ttggtactca
     9001 tcgaagaaga tgcctccgtg ccgaacgtct taaaactctg cggcgcggat ttgaaagtgg
     9061 tgtccgaaca gctcgccgcc agcgttgccg aaaacacccc cctgattccc gaacaccttt
     9121 tagacacggt cgaaacccgg cccacgctcg gcttccaacg cgtgatgcaa cgggcgatgg
     9181 tgcataccca gtctgccgga aaagccgcag tcgaaccgtt ggacgttttg gtcgcgctga
     9241 tgagcgaaac cgacagccac accgtctatt tcctcaagct gcaatcggtt acgcgttttg
     9301 aagttttgcg ctgtattgcc cacggctctc ccgatgaaga tgaagacgat ggcaactatt
     9361 cttcagacgg catggacgac gataatgaaa accgcaccaa accgggcaaa aaccctttat
     9421 cggcgtacac cgtcaacctc aacgccgaag tcaaagccgg ccgtatcgac cctttgattg
     9481 gtcgcaaaca cgaaatggaa cggctggtgc aaatcctatg ccgccgccgc aaaaacaatc
     9541 cgcttttggt cggcgaagcg ggcgtgggca aaaccgcgct ggcggaaggt ttggcacatc
     9601 aaatcgtcaa cggcggcatt ccagacgcgc tcaaagatgc cgaagtgtac gcgctggata
     9661 tgggctcgct gttggcgggc acgaaatacc gcggcgactt tgaagcgcgg gtcaaatccg
     9721 tcttgaaaca gctcgaaaaa atcccgcacg ccattttgtt tatcgacgaa atccacacca
     9781 tcatcggcgc aggcagcacc agcggcggca ccatggacgc gtccaacctg ctcaaacccg
     9841 cgctggcaaa aggttcgctg cgctgcatcg gcgcgaccac ttacgacgaa taccgcacca
     9901 ttttcgacaa agaccacgcc ttaagccgcc gcttccaaaa aatcgacgtg gtcgaaccca
     9961 ccgtttccga aaccgtacaa atcctgcgcg gcttgaagcc gatgtttgaa gccttccacc
    10021 aagtccgcta tacgcaaggc gcactcgaag ccgccgccga actctccgca cgctacatca
    10081 acgagcgttt cctgcccgac aaagccatcg acgtgatgga cgaagcaggc gcggcgcaac
    10141 ggattctgcc caaatccaaa cagaaaaaag tcatcggcaa agcacaaatt gaaaccgtca
    10201 tcgccaaagt tgcgcggatt cccgaaaaaa ccgtgtcgca cgacgacaaa caagtgctgc
    10261 aattcctcgg tcgcgatttg aaaaacatgg tttacggtca agaaaacgcc atcgacgcgc
    10321 tggttgccgc cgtcaaaatg tcgcgttccg gccttgccct gcccgacaaa ccgataggca
    10381 gcttcctctt ctccggcccg accggcgtgg gcaaaaccga agccgccaaa cagcttgcct
    10441 actcgctggg cgtaccgctg caacgctttg atatgtccga atacatggaa cgccatgccg
    10501 tatcgcgcct catcggcgcg ccaccgggct acgtcggctt tgaacaaggc ggccttttga
    10561 ccgaagccgt caacaaacag ccgcattgcg tattgctctt ggacgaaatc gaaaaagccc
    10621 accccgacat tttcaacgtc ctcctgcaag tcatggacgc aggcaaactg accgacaaca
    10681 acggcaagag tgccgatttc cgcaacgtca tcctgattat gaccaccaac gcaggcgcgg
    10741 aaagcctcag ccgccccagc ctcggcttta ccgccaagcg cgaacgcggc gacgaaatgc
    10801 aggcaatcaa caagctcttc acgcccgagt tccgcaaccg cttggacgcg attatcccgt
    10861 ttgcaccctt gtccgaaccc atcatcgtca aagtcgtgga caaattcctg ctccagctcg
    10921 aacaccagct cctcgacaaa aaagtcgaag ccgaattcac gtcggcattg cacaaatatc
    10981 tggcggaaaa aggtttcgac ccgcaaatgg gcgcgcgccc gatgcaccgc ctgattcagg
    11041 aaaaaatccg caaaccgctc gccgacgaac tcctgttcgg caaactatcc gacggcggct
    11101 tcgtgcggat agactgggat gcggcaaaag aagaagccgt gttgaagttt aagaaaagca
    11161 aggtcaaaat aaaaaccgcc tccgcataaa atcaaaaatg ccgtctgaaa ggttcagacg
    11221 gcattttgtc aaatatcggt ccctattccg gcaaacttct ccacaaacgc cgcaatcttt
    11281 tgaaaaacac tttgtttctt cgtcagatat tgcgggttga gcggactcat tttcggcagg
    11341 gtttcggtca gttccgtacc gttttcgctg gcatagccgc gtttgagcga gccggtcaaa
    11401 tagcgtttgg cggcggtttc gttcaggtct tcggcggcaa tcaatcctgc cgcttcgtgc
    11461 cgcattacct cttgcgcgta ggtgtaaaag gtttccagta tggcgggaac gtcggggact
    11521 ttatccaaat ccgtatcgtt gataaaatcc acaatcagac cctctttggc gcggtggccg
    11581 atgctggcgc ggatgatgcg gcggatttct tccaccagct ccgcttttcc tttgattttt
    11641 ttgtgatgct cgaaaaccag ttgcaagatg taatccaaat tgatttcctg cgatttgagc
    11701 aaatccacct caaaaaccac atcgtcccaa tcgatttttg actgttcttt ctgttcgcct
    11761 gctttttggc ggctcagcca gtctcggatg tcgttgtagg cggaacggta gtcctgcacc
    11821 gccctttcag acggcatcgg cacttgccgc atttcctgca cgtcttcatc gctcaggtag
    11881 tatttttcct gaaacgcctt catcgcatct tcgtccgccg catccacatt ctgcaactcg
    11941 cgcagcgcgg caaattcatc gtagttctgc aatacgtttt ccgcccgcag gtattcgccg
    12001 aagagtttgg caaaatcttt tttgtctttt tccgtttcga tttcgtcggg atcggggaaa
    12061 cgttcgcgca attcttttgc cacatccaaa tagccgcgcc gcgcttcgcc ggtctggctg
    12121 tcgatatagc cgttcatgta ttcttcgtag cttttttcca gcaccacgtt tttggtgttt
    12181 ttgtcgccga acagggtaat cgcatcaatg gtcgcctgct ccaaatcgcg gaagcagaca
    12241 atattgccga aggttttggt ggcatcgtaa atgcggttgg tgcgagaaaa cgcctgcatc
    12301 aggccgtgat agcgcaggtt tttatcgaca aacagcgtgt tcagcgtcgg cgcgtcaaaa
    12361 cccgtcaaaa acatgccgac cacaatcagc aaatctattt cctgattttt cacccgtttt
    12421 gccaaatcgc ggtagtagtt ctgaaaggct ttgctgtccg tgccgaaatt ggttttgaaa
    12481 caggcgttgt aatcgttgat ggcagcctgc aaaaattctt tcgcgctgct gtccatcgct
    12541 tccggttcaa aggtttcatc gacaatttca ccgacggcgt tctgctcttc gttggcagca
    12601 aaggaaaaaa tagtggccac tttcaacggg tgcaggctgc ctgcctgttg cgttttgaac
    12661 gcttcgtaat agcacttcgc cgcatccacg ctgctgacgg caaacatcgc gttaaagcct
    12721 ttgccgcccg cattcagccg gtgcgttttc tgcctgaact ggttcaggat atattgcgtg
    12781 atttcgcgga tgcgttcggg gtgcagcagg gcttggcggt tttcggcggc actcagtttc
    12841 ttctcgtcct gttccgcttc cacggctttg aactgcgggc gcacatcgtt gtaatccact
    12901 ttgaatttca atactttttc atcgcggatg gcatcggtaa tcacataaga atgcagctcc
    12961 cgcccgaaca cgcccgccgt ggtttccgcg cccaaagcgt tttcgggaaa aatcggcgtg
    13021 ccggtaaaac cgaactggca gaatttttta aatttctttt tcaggttttt ttgcgcttcg
    13081 ccgaattgcg agcggtggca ttcgtcgaaa ataaagacaa cccgctgatg gtaaaccggc
    13141 agattatctt cacttttcat caggttgttc agcttttgga tggtggtaac gatgattttg
    13201 ttgtcgtctt tttccaaatt gcgtttcaag cccgccgtgc tttccgaacc gttcacgctg
    13261 tcggtcgaaa aacgttggta ttccttcatc gtctgatagt ccaaatcctt cctgtccacc
    13321 acgaagaaaa ccttgtcgat aaatgccgat tccgtcgcca gacgcgccgc cttaaagctg
    13381 gtcagcgttt tgccgctgcc cgtggtgtgc caaacatagc cgccgctctc cggcttgctc
    13441 caattcttcg cctgcgccga gctgttgatt ttccacagaa tgcgttcggc ggcggcaatc
    13501 tggtacggcc gcataatcag cagcgtatca ttcgcatcga acacgctgta atgcagcaaa
    13561 acgctcagca atacgctttt ttgcaggaac gtggcggtaa agtctttcaa atccttaatc
    13621 ggatgattgt ccgaccgcgc ccaattcatc gtgaaatcga agctgttttt gtcgcgcttg
    13681 gtggtgttgg cgaaatagcg cgtgtccgtg ccgttggaaa tcacgaagat ttgcaggaat
    13741 ttgaacagcg aattttcgct gttgaagctc tctttgctgt aacggtgcac ctgattgaat
    13801 gcttcgcgca ccgccacacc gcgctttttc aattcaatct gcaccagcgg cagaccgttt
    13861 accaacacgg taacgtcata acggtttgca tgcgtgcccg tctgctcaaa ctggttgata
    13921 agctgcacat ggttgcgggc aaggtttttc ttgtccagca gataaatgtt tttcagacgg
    13981 ccgtcatcaa aagcgaaatc gtaaatatgg tcgtcgtgga ttttgcgggt tttatcggta
    14041 atattttcag acggtctgtc cagatattcc gtcaaaaacc gcgcccattc gccgtctgaa
    14101 aacgccacat cgttcagccg ctgcaactgc gcgcgcaggt tttccagcag cctgctttgg
    14161 ctgttcaaat ccttgcggta ttcgtaaccc tgattctgca aatcggcgat taactccgct
    14221 tccaaccggt tttccgattg gtagctgccc gactgttcga ttttttcata ttggtcgagc
    14281 acgatgaaat tcggcgtttc ggcgatgggt ttggtttcga ggttcatggg tcgtcctttg
    14341 cagtatcggg ttttaaacgg tttgatgttc ggattgggct gcctgaaaat gtgcaggctg
    14401 ctttttcagg tagccttagg gaaggcaagc agctgttcgc ggtaatattc gtattgtttg
    14461 cggcgcaggg cgatttcgtg cggcaggcct tcgctgatag agtgggtcag gatatcgaat
    14521 ttgtctagga tggcggcaat tttttcctgt tcggggagtg gtgggatggg gatttttaat
    14581 ttctttaatt ctttcatgct aataataggt aaacttgaag tattaataag ttttttgctc
    14641 tgctccgcaa taatgaaaaa ataatagaaa gcgaaattta tatctaaagc atccttaaat
    14701 tcatctttaa ttgtaaaatt ggtcagttgt tggttactaa tataattaac t
//
LOCUS       DAXHTR010000043        14559 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.043, whole genome shotgun sequence.
ACCESSION   DAXHTR010000043 DAXHTR010000000
VERSION     DAXHTR010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14559
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.043"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(35..250)
                     /gene="nqrM"
                     /locus_tag="ACFNML_000870"
     CDS             complement(35..250)
                     /gene="nqrM"
                     /locus_tag="ACFNML_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HFC6392279.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(276..1331)
                     /locus_tag="ACFNML_000871"
     CDS             complement(276..1331)
                     /locus_tag="ACFNML_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HFC6392280.1"
                     /translation="MPSETRLPNFIRVLIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRDKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQHTAGKPLRIS
                     SDFAHVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(1484..2701)
                     /gene="nqrF"
                     /locus_tag="ACFNML_000872"
     CDS             complement(1484..2701)
                     /gene="nqrF"
                     /locus_tag="ACFNML_000872"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HFC6392281.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVSVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPPHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(2715..3308)
                     /gene="nqrE"
                     /locus_tag="ACFNML_000873"
     CDS             complement(2715..3308)
                     /gene="nqrE"
                     /locus_tag="ACFNML_000873"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HFC6392282.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(3312..3938)
                     /locus_tag="ACFNML_000874"
     CDS             complement(3312..3938)
                     /locus_tag="ACFNML_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HFC6392283.1"
                     /translation="MADMKRLKHLMFSPFIDNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGMMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(3938..4714)
                     /locus_tag="ACFNML_000875"
     CDS             complement(3938..4714)
                     /locus_tag="ACFNML_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HFC6392284.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSVIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKDTDIGKTFAERIEQRVVDLATGEYVADAPKDFSARIAGKDPA
                     QSIRIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(4707..5939)
                     /locus_tag="ACFNML_000876"
     CDS             complement(4707..5939)
                     /locus_tag="ACFNML_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HFC6392285.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGVQAFGALTPDLLQQNIANDWHYTLAD
                     ALGINMSSEASVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(5942..7285)
                     /locus_tag="ACFNML_000877"
     CDS             complement(5942..7285)
                     /locus_tag="ACFNML_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HFC6392286.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPAITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPASGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAAPFAIFVNAMDTNPLA
                     ADPVVVIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVVALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LFGWVAPQPDKYSITRTTLGHFLKNKLFKFTTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(7633..9054)
                     /locus_tag="ACFNML_000878"
     CDS             complement(7633..9054)
                     /locus_tag="ACFNML_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HFC6392287.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWGLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGTLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(9412..9771)
                     /locus_tag="ACFNML_000879"
     CDS             complement(9412..9771)
                     /locus_tag="ACFNML_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HFC6392288.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(9768..10373)
                     /locus_tag="ACFNML_000880"
     CDS             complement(9768..10373)
                     /locus_tag="ACFNML_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HFC6392289.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDGFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGILQIAPFHPQFRFQNTEPDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(10420..10902)
                     /locus_tag="ACFNML_000881"
     CDS             complement(10420..10902)
                     /locus_tag="ACFNML_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HFC6392290.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      11106..11203
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            11414..12514
                     /gene="gcvT"
                     /locus_tag="ACFNML_000882"
     CDS             11414..12514
                     /gene="gcvT"
                     /locus_tag="ACFNML_000882"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFC6392291.1"
                     /translation="MITLKTTPFHQAHQDAGAKLVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYCIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVNNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAVAFFKALQQA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDDTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLDKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     gene            12675..13061
                     /gene="gcvH"
                     /locus_tag="ACFNML_000883"
     CDS             12675..13061
                     /gene="gcvH"
                     /locus_tag="ACFNML_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFC6392292.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            13208..14455
                     /gene="hemA"
                     /locus_tag="ACFNML_000884"
     CDS             13208..14455
                     /gene="hemA"
                     /locus_tag="ACFNML_000884"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFC6392293.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEVRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHDYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIKALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSVQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
BASE COUNT         3532 a         3586 c         4010 g         3431 t
ORIGIN      
        1 gtactggttt ttgttaatcc actatatttc ggcgttaacg gtcaacccgt atgccgcctg
       61 cctgtttttc ttcatccagt ttcttttgca gggtgtcgca aggtgtgtcg cagtcgcaca
      121 tttttttcat acccaaggca gtaatgccgc cgcaactgcc tttgatgctg cgtttggaga
      181 aaatatagcc gaccgccata ccgatgatga cggtcaggaa gatgccgaag gtaaggagca
      241 gggttttcat ggtgtttcct aatcggtttg tatgtttagc ggagcagttt ttcaaattcg
      301 gaagacatgg cggtgcggta gccgccttta tccctgacaa tcaggaaaac agcgagtttt
      361 tcgcgctctg ccagctttaa ggcttcggtt tcgcccaata cgaataatcc tgtggacaag
      421 ccgtccgccg tcatcgcact gtctgcgacc acgctgatgg aggcgaggtt gtggctgatg
      481 ggtcgtttgt tgttcgggtt gatgatatgg gagaggcgtt tgccgttttt atcgacgtgg
      541 aaaatacggt aatcgccgga agtggcaagc gaacggttgt tcagcgggac gataatctgc
      601 gtattgccgc cttggacgat attgggctgc tcgataccga tgcgccacgg ttcgccgcgc
      661 gcgtttttgc ctttgccgtg caactcgccg ccgatttcga ccagataatt ttgaatgccg
      721 tatttttcca gttcgcccgc aactttatca acgccgaagc ctttggcaat cgaagataaa
      781 tccaaatagg ccttggggtg ggttttgctc aaggaagcgt aatctttgcc ttgtttcaaa
      841 atgattttgt ctatgcccgt ataagatgcc gcctgtttga tttgttccgg cgacggttca
      901 cgggtaacgg atttgtcggg gccgaatccc caaaggttga ccaaggggcc gacggttacg
      961 tccagcgcgc cgtgtgtcag gcggttcagg cggacggctt cggcagtaac gtgtgcgaag
     1021 tcgcttgaaa tgcggagggg cttgccggct gtgtgttggt tgaaccggct gatttcggag
     1081 tcgggctgat aggtggacat ctgccggttg acttctttaa gcgcgtcatc gatgcgtttt
     1141 tgtatttcgg caggtgaggg gagtttgtcc cgattatttg aaaggtattt gacggtatag
     1201 gtcgtgccca tcgtttcgcc ttgcagggta acggtttgcg cggtttgttc cgaacaggcg
     1261 ttcaggaaga tgaaacccag ggcaaatatc aagacgcgga taaagttcgg caggcgtgtt
     1321 tcagacggca tagtgtttga cggttttggc aaatggtttg aattatatcg caaaacggcc
     1381 ggtatgtttc tatgccgatg ccgtctgaag ggtgttcgga tggcatcggc atagaaaaag
     1441 gaagaaaccg aggtttcttc cttttgtatt tgaagccgaa tatttaaccg ccgaaatcgt
     1501 ccaagaggat gttttcgtct tccacgccca agtctttgag cattttgatg acggactggt
     1561 tcataatcgg agggccgcac atataaaatt cgcagtcttc cggtgcttcg tggtttttca
     1621 ggtggttttc gtaaaccacg ttgtgaatga agcccgtgta gccgtcccag ttgtcttccg
     1681 gcagcgggtc ggacagggcg acgtgccacg tgaagttcgg gaactctgcc gcgagttggt
     1741 caaagtcttc gacatagaac atctcgcgtt tggaacgtgc gccgtaccag aaggtaatct
     1801 tacgtttgga gttcaaacgt ttcaactggt cgaaaatgtg ggaacgcatc ggagccatac
     1861 ccgcaccgcc gccgataaat accatttcgg catcggtgtc tttggcgaaa aattcgccga
     1921 acgggccgga aatcgtaact ttgtcgccgg gtttgagcga ccagatgtag gacgacattt
     1981 gtcccggagg cgcatcaggt acgcgcgggg gcggcgtggc gatacgcacg ttcagcataa
     2041 tgatgccttt ttcttcagga tacgaagcca tagagtaggc acgcaaaatc ggctcgtcca
     2101 ctttggaaac gtattgccac aaattgtatt tgtcccagtc ttcgtgatat tccttaggaa
     2161 tgtcgaagtc tttgtaggca acagtgtgag gaggagcttc aatttgaatg tagccgccgg
     2221 cgcggaaggg gacttcttcg ccttcgggaa tggcaagctt gagttcttta atgaacgtgg
     2281 ctttgttatc gttggagatg acggtgcatt cccatttttt cacgccgaac acttcttcgg
     2341 gcacttcgat gtccatgtcg gttttgacgc tgacttggca cgacagacgg cagccttcgc
     2401 gtgcttcgcg tttgctgatg tgggacagct cggtcggcag gatgtcgccg ccgccgcttt
     2461 ttacgacgac gcggcattgt ccgcacgaac cgcccccgcc gcaggcggag gggataaaga
     2521 tgccttcgtt ggcaagcgcg cccaagagtt tgccgccggc gggcatcgtc agctcttttt
     2581 cgccgttgac tttgatggtg atgtcgcctt cgctgaccag tttggatttg gcaaatagaa
     2641 tcatcagtgc caaaaccaaa acgatgacgg taaacatcac gatacctaaa ataatctcca
     2701 taccggtccc tttcttataa ctggatgccg gagaacgaca taaacgccat cgccatcagg
     2761 ccggcggcga taaaggtaat gcccaagcct ttgaggcctt tgggagcgtc cgaatatttc
     2821 attttttcgg taatgcccgc caaagcgaca atcgccaaca tccagcccaa gcccgcgccg
     2881 aagccgtata caacggactc gccgaagttg tattcgcgtt gcgccataaa cgatacggcg
     2941 ccgaaaatcg cgcagttcac ggtaatcagc ggcaggtaga tgcccaacgc gttatagagg
     3001 gcggggacga atttatccaa gaacatttcc aaaatctgca ccaaagcggc aatcacgccg
     3061 atgaaggtga tgaatttcaa aaaggtcaaa tccacgcctt cgacaatcgc gccgtctttg
     3121 agcagcgagt aaacgagttg gttgacaggg acggacagcc cgagtacgaa aattaccgcc
     3181 acacccaaac cgaatgcggt ggatactttt ttggataccg ccaaaaacgt gcacataccc
     3241 aaaaagaagg atagtgccat attttcaatg aagacggatt tgatgaagag gctcaaatag
     3301 tgttccatag cttattcctc cgcctgttcg ggtttccagg tacgcagtcc ccaaatcaaa
     3361 aagccgatga tgaagaacgc gctgggggcg agcaggaaca agccgttggt ctgataccag
     3421 ccgccgtcct gcacggtttg gaaaacggtg tagcccaaga gtttgcccga gccgatcagt
     3481 tcgcggacgg tggcgacgac aagcagcatc atcccgtagc ccgcgccgtt gccgatgccg
     3541 tcgatcaggc tttccagcgg cggctctttc atcgcaaatg cttcggcgcg gcccatcacg
     3601 atacagttgg taataatcag accgacgaat acggaaagct gtttggacaa ttcgtaggca
     3661 aatgcctgca agagttggtc gaccagcgta accagcgacg cgataatcgc catttgcacg
     3721 ataatacgga tgctgttggg gatgtagttg cgtaccagcg aaatgaagaa gctggaaaaa
     3781 ccggttacca aagctacgga aatacccatc acgatggccg tctgaagttt ggtggtaacc
     3841 gccaaagccg aacaaatacc caaaacctgc aaggcaatcg ggttgttgtc gataaagggt
     3901 gaaaacatca aatgtttcaa gcgtttcata tcagccatta ttgcgctcct gctgatttca
     3961 atttgttcag gtaggggata tagccgtttt cgccgaacca gtaggcgaac gaaccttgca
     4021 cgcctttgga tgtcagcgat gcgccggaga gggcatctac gccgtgttct ttgtccgaac
     4081 ccgcgccttt gccgacgtgc agggcgagtt tgccttgtcc gtcaaacagt tttttgccga
     4141 cgaatttttg ctgccacaac ggattgccga tttcgccgcc caagcccggg gtttcgcctt
     4201 gttcgtagta ggtaatgccg ttgatggtgt tgccgtcggg ctggatggcg acaaagccgt
     4261 acatgaccga ccacaaaccg ttaccgtgca taggcaggat gatttgcccg attttgccgt
     4321 cttcgccttt taccaaataa acctcggtgt atttggcacg gcttttgatg cctgccaaat
     4381 cgtcttcggg tttgatgcgg atgctttggg cagggtcttt gcctgcgatg cgcgcgctga
     4441 agtctttagg cgcatcggcg acgtattcgc cggtcgccaa atcgacaaca cgttgctcga
     4501 tacgctcggc aaaggtttta ccgatgtcgg tgtccttatc catcaaaccg gctacgctca
     4561 agatatagcc ttgtttgtct tggagttttt gtttctcttg gatgggtttc aagccgacga
     4621 ccgcaccggc aacgatgacc gagcaaatca ggctgaccgc caacacgaca atcagcgtac
     4681 cgctgaagct gtctttatcg aatttcttag ccattgctgc gcgcctttct gcgtttgatg
     4741 ttcgcttgtg cgacgaaata gtcgaaaatc ggggcaaaca ggttggcaaa cagaatcgcc
     4801 aacatcatgc cttcggggta agccggattg accacgcgga ttaatacgca catcacaccg
     4861 atcagtgcgc cgtaccacca tttgccgaca ttggtaaagg aagcggaaac ggggtcggtc
     4921 gccataaaca gcataccgat ggcgaagccg ccgacgacca aatgccagta ccaaggcata
     4981 gcaaacatag cgttggtgtc cgaaccgatg aagttgaaca gcgaagacat cgcaatcata
     5041 ccgatcatca cgccggcaat aatgcgccaa gaagcgatgc gggcaaacac gataaacgcg
     5101 ccgccgatta agagtgccaa agtggagact tcgccgatgg agccgggcag tttgccgata
     5161 aacgcgtcca tccaagtgat ggtttgaccg gttacggcgt ttttcaggcc gtctgcaccg
     5221 tgtgccgccc attgcgccag cgcggttgcg ccggaatagc cgtcaaccgc cgtccaaacc
     5281 gcatcgccgc tcaagttggc ggggtaggcg aagaacagga aggcgcggcc tgccagcgca
     5341 gggttcataa agtttttacc cgtaccgccg aatacttctt tcgcaaccac aacgccgaaa
     5401 gaaataccca aagccgcctg ccacagcggc agcgtgggcg gaacgattaa ggcaaacaga
     5461 atcgaggtaa caaagaaacc ttcgttgatt tcgtgtttgc gcacggtggc gaacaagact
     5521 tcccagaaac cgccgacggc aaatacggtc gcgtaaatcg gcaggaagta aatcgcgccg
     5581 aacagcattt tgcccaacac gctcgcttca gacgacatat tgatgcccaa agcgtcggca
     5641 agggtgtaat gccagtcgtt ggcgatgttt tgttgcagca aatcaggcgt taacgcaccg
     5701 aatgcctgta cgccgacgtt gtacataccg tagaacatgg cagggaacaa agccagccac
     5761 accaaaatca tcatgcgttt ggagtcgagc gcatcgcgga cgtgtgccgc tttgcgcgtt
     5821 accgcaccgg atgtatagaa aatcgtcgcc gcagcttcgt agagggcata ccatttttca
     5881 tgtttgccgc ctggcaggaa gtgcggttcg attttttcca gaaaatgttt caagcccata
     5941 atcagccttc cttctcaatg gtttccagca ccttacgcaa cagcgggccg tattcgtatt
     6001 tgcccgggca gacgaagctg cacaaagcga ggtcttcttc gtccaattcc aagcaaccca
     6061 atgcctgcgc gctgtcggta tcgccgacga ttaaatcgcg caaaagcagg gtaggcagga
     6121 tgtctagcgg cattacgcgc tcgtaagtac caatcggcac catggcgcgg tcgccaccgt
     6181 tgacggctgt cgtgaacttg aagagtttgt ttttcaggaa atgaccgagg gtcgtacgcg
     6241 tgatcgagta tttgtccggt tgtggcgcaa cccagccgaa cagttcttta ctgcggcctt
     6301 cttcgataac ggaaatctga ttgtggtagc gtcccaaata atcgtgcgcg ccttgtgcaa
     6361 tcgcgccgtt caataccgaa ccggaaatca cgcggttgtc tgcgtcaacc aattcgcccg
     6421 cagtaatttg cgatactttc gcacccaaaa cggtacgcaa gaggcgtggt ttgttgactt
     6481 gagaaccacc caaggcaacc acgcgctcgg tgttcagacg gcctgttgca aacaaacgtc
     6541 cgatggcaat tacatcttga taattgatgg tccaaacggt tttgttcgca ccgacaggct
     6601 caatgaaatg aatgtgcgtg ccactcaaac cggcaggatg cgggccgcca aattcatgag
     6661 tttcgatgtt ggcggcgttt tcagacggca cgtctgcgcc tgctgcttta cacacatgga
     6721 ttttacgctc ggtcaaacgg ctcaatacca gcaaacctcg tctgaaatcc tcggcggctt
     6781 ctttgatcac aaccacaggg tctgccgcca gcggattggt gtccatcgca ttgacgaaga
     6841 tggcgaacgg cgcggcatcg acggcaggga ttttgctgaa cggacgggta cgcagcgcag
     6901 tccacaaacc ggattggatc agattgcggc gcacttcttc gccgcttaag tttgccaacg
     6961 cttcgggtgc gtagcgttca aactcgattt cgtcgttgcc ttcaacggca atcacgactg
     7021 actgaagtac gcgcttttcg ccgcgatgga tggcggcgat tttgccagaa gccggcgcgg
     7081 taaacaccac gcccggattc tttttgtctt caaacagcac ttggcctttt ttgacggcat
     7141 cgccttcctt gactttcatc gaggggcgca taccggcata ttcttcgcca agcaacgcga
     7201 cttcggtaat ggccgggccg tcataaatga cttgctccgg tctgcccgcg atgggcaggt
     7261 ttagaccttt tttgatttta atcatatatt tgcattactt gtgatggtta aggtaaaaac
     7321 ggcgtgtttt gataccgtgt cgcgtggcat caaaagcatt gaataaatta atgtagcaaa
     7381 gtgttagatt ctatcaggaa ttgtacctgt ttgtcagatt tgctgctttt ttccttgcgg
     7441 aagccgtttt tatagtggat taaatttaaa ccagtacggc gttgcctcgc cttgccgtac
     7501 tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataattg
     7561 tcggaagggg ggatattgat ttgattatgc cggaatttaa aatgccgtct gaatgttcag
     7621 acggcatagc gtttacagca gtttgaaaac gaaaaagata agggtatgta cgatgaagac
     7681 gggtgtcagg aaggcgaccg accacatcat atagccgaag aaagtcggca tcggtacgcc
     7741 gcgctgttcg gcaatggcct tgaccatgaa gttcggtgcg ttgccgatgt aggtcagtgc
     7801 gcccatgaat accgaaccca tagaaaccgc cagcagcgaa tgaaacaggg tacccgtcat
     7861 caaggcttgg gcatcgccgc ccgccatatt gaaaaaaacg agataagtgg gcgcgttatc
     7921 caagaatgcc gacaatatgc cgctcatcca aaaatacatc acattaatcg gatgacctgc
     7981 cgtatcgtga accagcgata ccaccccgcc cagcgcgcct gcctcgcctg ctttcagaat
     8041 gctcaggacg ggaaagatgg tgatgaagat gccgaggaag agtttgccca cttcggcgat
     8101 gggttcaaag ttgaattcgt tgcctgcgcg gacttgtttg ggcgtgattg ccatagatac
     8161 ggcggtcaat gcaatcagga tgacatcgcg gacgaggttt tgcagggcgt aacggctgcc
     8221 gaggatttca aatcccgggt gttcgggttt ccaaaggccg gacattagaa ccgcgccgac
     8281 cacgcccgaa agcaggagga agttccattt gccgaagatg gcgatttttt cgggtttttc
     8341 ctgttgtgcc ggcgtatctt gtgcaatgct ttcctgtttg aagaaacggt tgtcgatgaa
     8401 atagaaggcg gtcaacagga cagcggtgct gatcaggacg ggggcgaaca tatgtttgac
     8461 cgtccacatg aaatctacgc ctttgaggaa gccgaggaag agtggggggt cgcccaaagg
     8521 ggtcagaccg ccgccgatgt ttgcaaccaa gaaaatgaag aagatgacga tgtgcacgcg
     8581 gcgggtacgg ttttggttgg ctttcagcag cggacgaatc atcagcattg ctgcgccggt
     8641 cgttcccatg atagaggcaa gtgcagtacc gacggcaagc agggcggtgt tgagcttggg
     8701 tgtgccgttc aagtcgcccc aaaccaaaat gccgcctgaa atggtgtaca gggcaagcag
     8761 cagcaggatg aaggggatgt attcttcaac gagtgcgtgt gcgacggtat ggataccggc
     8821 ggacgcgcca aaaaccaaac tgaacgggat gaggaagagc aatgtccaaa aggcggtaat
     8881 tttgccgtaa tggtgatgcc aggtatgcga aaaaaacaag ggacccaatg cgatagacag
     8941 caaaatcagg gcaaagggca ggccccacag caggtttagg tttgcgccgt ccaaatctgc
     9001 ggcgtaaacc gatgctggga aaagcattag tgaaaacagg ggtaggtggc gcatcgtgtt
     9061 tcctcgattc aagcactgcc ttgcgcggcg cgtgggagtg atacaggcac cgtgccgccc
     9121 ggacataggc ggactgtgct gcctgtttgt ttttgaaaaa agttttccga ttgattgtaa
     9181 agtaaataat caggataaac cagtttccca aaccggaagg cggcgggaag gcggattgcc
     9241 gtgcttggga atatatgttc tttttgataa ataattttat ttaaaacaaa taaatatatg
     9301 tctttaataa ataatctatc gaaaacgaaa aatgaattta ttttaacata tatttgcaat
     9361 gaaacaggtt tgccccccgt ttgtttgccc ttatcccttt cagtacggca ttcaagattc
     9421 gggcctgcgc cacatccata tggcgacaag ggaacaaaaa accgatgaaa ccgccccgac
     9481 ccaccagcgt tggggaaact gccaaaacat tatcaggcag gatgcggtca tcatactgat
     9541 ggcgaatatt ttggctttgc gcggcactgc gccgttttgt tcccagttat gaaccatcgg
     9601 accgaaatag cggtgccggt gcagccagcg gtgaaagcgc ggggatgcct ttgcccagca
     9661 ggcggcggag agcagtacga acggcgtggt cggcaacagc ggcaaaaaaa tgccgatgat
     9721 acccaacagc agggaaatgc agccgcaggc aattaaaaga taacgtatca ttttgaaata
     9781 tttttcttat tgtgcggata agggcaggat gtgataccga gttttgccca gccttcatgt
     9841 cccatttttt ccagcagggc gatattgcgt tcgaatattg ccgaagcgtc gggaaaggct
     9901 tgtgcggctt tggcaatgct gtcttcgcgg atcagatgca gtgtcggata gggagaacgg
     9961 ttggtgtagt tgccgatgtc gtcgggttcg gtattctgaa agcggaattg gggatggaac
    10021 ggtgcgattt ggaggatgcc ttccaagccg ttttcgacaa cggcggcatc ggcaatatcg
    10081 agcatatcgt tgaatacgtc gaaatcgggg aatagggtcg ggtgaaccag cagggtggtt
    10141 tccagttcgg tggcgggtgt attgcccagt cgctgcagtt cttcgtccaa gtcttccaaa
    10201 aaaccgtcaa ggtgtttggc ttcgctgatc gcgatgcgga caaggttttt aacgtggggg
    10261 gctttggcaa agggacacag gttcagaccg atgacggctt tttccaacca ttgtccggtg
    10321 tgttcggcaa cagcatcttt attttcggaa gtattgatat tcattattgt catgtaaatg
    10381 tgtttgcaga ttgcacgtgc gggaaaatcg ggaagggcac tattccttca gcaggtggtt
    10441 gagtggcagg gaggtggtgt gtttgatttc ttttaaaaca aagctcgatt gggcatcctg
    10501 tacgccgtgg tgggacagga gcgtatccaa aacaaaatgg gaaaacgcgt tcatatcggt
    10561 aaaaaacgcc tgaagcaggt agtcggtttc ccctgtcagg gcgaagcagc tcaagacttc
    10621 aggccatttt cgaaccgatg cggcaaagtc ttcccgcgcg tcttttgctt tgcggatgga
    10681 aacgcggata aatgcctgaa gccccaggtt gacggattcc ggagacagca gcgcggcata
    10741 ttggcggacg atgccggcat cttccaactg cttcagacgg cgcaggcacg gagaaggcga
    10801 aagtgcgaca cgttcggaca gttcgacatt ggtcagcctg ccgttttcct ggagaacctg
    10861 taagatttta atatcggttt tgtctaaagt gagttggggc atatttgcgt tccgttttaa
    10921 ggaattcgga ttgtctgtcc gtatgtttgc ggcaatccgc acagatggag accatattaa
    10981 catataaaaa gttataccgt catccgggac aaattttgtt ttcggaaaat catgtgaaaa
    11041 cagaggcggt cggtttgcat ctcttttaag acggcttgcc caaaccgccg attcaagaca
    11101 taatcgggaa atgtgcagga gagtgttaca cccaactaca atgtaaccac cgaaggcgca
    11161 gacaccctta aatcgctcag gtatcaggga ctgcacattg aaacaaacaa tctggagagc
    11221 ggcgttggaa taacgtccac cgaaggggag aaggccgtct gaaccaccat tcagacaacc
    11281 gcgcaaagca gtgagcagac tggtttgcca tcatgcggat acggccgaaa atctcaggtt
    11341 caaggacaga tagggtcatc cgcgcacagg tgcgcgggtg gcatctgaac aaaaaatccg
    11401 gagaaacttg agaatgatta ctctgaaaac caccccattt catcaagccc atcaagatgc
    11461 aggcgcgaag ctggtagatt ttgccggctg ggagctgccc atccattatg gttcgcaaat
    11521 cgccgaacac gaagccgtgc gcaccgacgc aggtatgttt gacgtatccc atatgcttgt
    11581 taccgacgta gcaggcgcaa atgccaaagc ctttttccgc aaattgattg ccaacgatgt
    11641 cgccaagctc gcttttgtcg gcaaagccct ttattccgct ttgctcaacg acaacggcgg
    11701 cgtgattgac gacttgatcg tttaccgcac caatgaagcc gaaacccaat actgcatcgt
    11761 gtccaacggc gcgactcgcg aaaaagatac ggcacaattc cacaaagtcg gacaagagtt
    11821 cggcgtttct ttcaatccgc gctacgacct cggcatgctc gccgtacaag gcccgaaagc
    11881 catcgagaaa ctcctgaccg tcaaacccga atgggcagat gtcgtcaaca acctcaaacc
    11941 gttccaaggc gcggatttgg gcaacgactg gtttgtcgcc cgcaccggct acaccggcga
    12001 agacggcgtc gaagtcatcc tgcccggcac cgaagccgtc gcattcttca aagccctgca
    12061 acaagccggc gtacaaccct gcggcctcgg cgcgcgcgat accctgcgca tggaagccgg
    12121 tatgaacctc tacggcaacg atatggacga cgacaccagc ccgctcgaag caggcatggg
    12181 ttggacggtt gatttgaaag acgaaagccg cgattttgtc ggtaaagccg ccttgctggc
    12241 attgaaagaa aaaggcgttg ccgtcaaaca ggtcggtctg ttgctcgaca aaggcggcat
    12301 cctgcgcgcg catatggaag tgttgaccga caaaggcaaa ggcgaaacca ccagcggcgt
    12361 attctctcca agtctgaaac aatccatcgc catcgcccgc gtgccgaaag actttgacgg
    12421 cgataccgcc aaagttctga ttcgcggcaa agaagtggac gtacgcgtac tgaagctgcc
    12481 gtttgtgcgc aacggacaga aacagtttga ttgatgcggt ttcagacggc attttcattt
    12541 catatgccgt ctgaaagcag gttttaattg ttgtccgata cggacgtttg tagaaagcat
    12601 tgaacaaggc atctgtggat attgattcat gcagatgccg tctgaaaata acccctatca
    12661 atgagtatca aaccatgagc aacaacatcc cggccgaact gaaatacgtt gccagccatg
    12721 aatggctgcg ccttgaagaa gacggtacga ttaccgtcgg cattacccac cacgcgcaag
    12781 agctgttggg cgacatcgtg ttcgtcgagc tgcccgaagt cggcgcgaac cttgccgctg
    12841 aagagcaagc cggtgtggtt gagtctgtaa aagccgcgtc cgacgtgtac gcaccgattg
    12901 caggcgaagt cgttgccgtc aacgaagatt tgccaagtgc tccggaaact gccaacagcg
    12961 attcttacgg cgcaggctgg ttcttcaaaa tcaaacctgt caatcctgcc gattacgacg
    13021 gtctgctgac tgccgaacaa tacgcgggcg aagtggacta aatctctatc ctgataatgg
    13081 gatgactgat ttgagaaact gcactttcag acggcaagga tgcgtttgga agtgcagttt
    13141 ttgtctgtgt cttcaaagtt cagttaggct ataataacgg tctatattca tctttaccga
    13201 ttttttcatg caacttaccg ctgtcggact caatcatcaa accgcacctt taagcatacg
    13261 ggaaaagctg gcgtttgccg ccgccgccct gcctaaagcc gtccgcaatc ttgcccgaag
    13321 caatgcggca acggaggcgg taatcctttc tacctgcaac cgcaccgagc tttactgcgt
    13381 cggtgattcg gaagaaatca tccgatggct tgccgattac cacagtttgc cgattgaaga
    13441 aatccgtccg tatctgtacg cgctggatat gcaggaaacc gtgcgccatg ccttccgcgt
    13501 tgcctgcggc ttggattcga tggtgttggg cgagccgcag attttaggac agattaagga
    13561 tgcggtcagg gttgctcaag agcaggaaag tatgggtaag aaactcaatg ccctgttcca
    13621 aaaaaccttt tctgttgcta aagaggtccg taccgatact gccgtcggcg aaaactcggt
    13681 ttccatggct tccgcttccg tcaaattggc ggaacagatt tttcccgaca tcggcgattt
    13741 gaatgtcttg tttatcggcg caggcgaaat gattgagctg gttgccactt attttgccgc
    13801 caaaagtccc cggctgatga cggttgccaa ccggacgctg gcgcgtgcac aggagttgtg
    13861 cgacaagctc ggtgtcaacg ccgaaccgtg cctgctgtcc gatctgcctg ccattctgca
    13921 cgattacgac gtagtggttt cttcaacggc aagccagttg cccattgtcg gcaaaggcat
    13981 ggtggagcgt gcattgaaac aaaggcagag tatgccgttg ttcatgcttg atttggcagt
    14041 gccgcgtgac attgaagcgg aagtcggcga tttgaatgat gcctatcttt atacggtgga
    14101 cgatatggtc aacatcgtcc aaagcggcaa ggaggcaagg cagaaggccg ccgccgccgc
    14161 cgaaacgctg gtatccgaga aggttgccga atttgtcagg cagcagcagg gcaggcagag
    14221 cgtccccttg attaaagcct tgcgggacga gggcgagaaa gcgcgcaagc aggtgttgga
    14281 aaatgcgatg aaacagcttg ccaaaggcgc aacggcagaa gaggttttgg aacggctgtc
    14341 cgtccaactg accaacaagc tgctgcattc gccgacccaa actttgaata aggcggggga
    14401 agaagataaa gatttggttc atgccgtcgc gcagatttat catttggaca aataacggtg
    14461 cgccggaaaa ggatgccgtc tgaaggggtt tcagacggca tatttttgtg ctgtctgaca
    14521 acatcgtgaa atcccacatt atatcgatgt aatcacaaa
//
LOCUS       DAXHTR010000044        14392 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.044, whole genome shotgun sequence.
ACCESSION   DAXHTR010000044 DAXHTR010000000
VERSION     DAXHTR010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14392
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.044"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            25..1194
                     /locus_tag="ACFNML_000885"
     CDS             25..1194
                     /locus_tag="ACFNML_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6716 putative glycosyltransferase"
                     /protein_id="HFC6392294.1"
                     /translation="MNKKRVLLIASYDSFLNTGYAVAKEINYSTIDIYIHQTKKNILS
                     ESQLSLNGLSLKEINFFHIDDYFDKLMYQNYDIVILSLGNGLLTRFFRNSSKLNLTLP
                     IVITLFPGVIFGDSESIITRLGANILLFNNYYDYQIGNKFKERYNLSCTNILYGYPIL
                     RKKHHCSDGNKVYFIDQVKIPDGKKERLLILKKLIALANRYPHKDLVLLLRVHQNDIT
                     VHEDKYSYAELAKNMKLPDNFKIERKRTQDALQEMGYCLSFSSTMLFEAESCGIPIGV
                     ISDLGVDKKYANTHFKDSGVLVNLDTVDLDSPNTINPTWAELYTNPDSLSSKEFNQLL
                     EVTSTQPPSFDNYFIAVNDVQPVSMVLLRKFKKLIYHPKQFFVDSKLYKLIKFTK"
     gene            1242..2105
                     /gene="galU"
                     /locus_tag="ACFNML_000886"
     CDS             1242..2105
                     /gene="galU"
                     /locus_tag="ACFNML_000886"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFC6392295.1"
                     /translation="MQKIRKAVFPVAGMGTRFLPATKASAKEMLPIVDKPLIQYAAEE
                     AVAAGCTELIFITGRNKRNIEDHFDKAYELETELEMREKHKLLGYVQKILPDHVSCIF
                     IRQAEALGLGHAVLCARPAVGNEPFAVILADDLIDAPQGALKQMVDIYNQSGNSILGV
                     ETVDPSQTGSYGIVEVEQLKSYQRVTNIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     NLPRGAGGEIQLTDGIARLLDHEFVLAHAFEGKRYDCGSKLGYLEATVAYGLKHPELK
                     DDFKELLKQFV"
     gene            2188..3204
                     /gene="galE"
                     /locus_tag="ACFNML_000887"
     CDS             2188..3204
                     /gene="galE"
                     /locus_tag="ACFNML_000887"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFC6392296.1"
                     /translation="MNILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPRL
                     KTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPIKYYDNNVS
                     GSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPPGDTTSPYGASKSMVERIL
                     TDIQKADPRWSMILLRYFNPIGAHESGLIGEQPNGIPNNLLPYICQVAAGKLPQLAVF
                     GDDYPTPDGTGMRDYIHVMDLAEGHVAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAF
                     EAASGLTIPFEIKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNHPN
                     GYDD"
     gene            3300..4361
                     /gene="rffG"
                     /locus_tag="ACFNML_000888"
     CDS             3300..4361
                     /gene="rffG"
                     /locus_tag="ACFNML_000888"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFC6392297.1"
                     /translation="MKKILVTGGAGFIGSAVVRHIINDTQDAVVNVDKLTYAGNLESL
                     ESLANHPRYAFEQVDICDRAALDRVFAEHQPDAVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWHDMPSEKKAAFRFHHISTDEVYGDLHGTDDLFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYG
                     DGMQIRDWLFVEDHARALYQVVTEGEIGETYNIGGHNEKANIEVVRTICALLEELVPN
                     KPQGVARYEDLITYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLNN
                     QTWWSRVLDGSYNRERLGS"
     gene            4574..5449
                     /gene="rfbA"
                     /locus_tag="ACFNML_000889"
     CDS             4574..5449
                     /gene="rfbA"
                     /locus_tag="ACFNML_000889"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFC6392298.1"
                     /translation="MKGIILAGGSGIRLYPITRGVSKQLLPVYNKPMIYYPLSVLMLA
                     GIRDVLVITTPEDNDAFRRLLGDGSDFGIQISYAIQPSPDGLAQAFLIGEEFIGDDSV
                     CLVLGDNIFYGQHFSQSLKEAAASVETKGATVFGYQVKDPERFGVIEFDENFKALSIE
                     EKPKQPKSDWAVTGLYFYDNRVVEFAKQVKPSERGELEITTLNQMYLDDGSLNVQLLG
                     RGFAWLDTGTHASLLEAASFVETIENIQNLQVACLEEIAWRNGWLSSEEIRNLAKPMC
                     KNEYGRYLLRLVGKD"
     gene            5504..6043
                     /gene="rfbC"
                     /locus_tag="ACFNML_000890"
     CDS             5504..6043
                     /gene="rfbC"
                     /locus_tag="ACFNML_000890"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HFC6392299.1"
                     /translation="MNIIDTRIPDVKLLEPKVFGDERGFFMETFRDEWFRQNVAERTF
                     VQENHSKSTQGVLRGLHYQTENTQGKLVRVVSGEVFDVAVDMRRDSPTFGHWVGEVLS
                     ADNKRQLWVPEGFAHGFYVLSDEAEFVYKCTDYYNPNAEHSLIWNDATVGIDWPLLGE
                     PSLSAKDLAGKMLAEAATF"
     gene            6205..8253
                     /locus_tag="ACFNML_000891"
     CDS             6205..8253
                     /locus_tag="ACFNML_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HFC6392300.1"
                     /translation="MIPAYIPLRSFGIRKIPNITQFLPEYSLNKTVFNAQTIIGWGLR
                     PTTHQARAFAQSHGLPFVALEDGFLRSLGLGVAGYPPFALVYDDIGIYYDTTRPSRLE
                     QLILAANMPSEMLNQAQQAIDLILTHRLSKYNHALDFSDGLVQDSKNIVLVIDQTFGD
                     MAIQYGGADEATFRQMFQTALAENPDAEIWVKTHPDVLSGKKQGYLTDLAQQPRVRLL
                     AEDINPISLLQAVDKVYCVTSQMGFEALLCGKPLVTFGLPWYAGWGVSDDRHPEINRL
                     VQTQRRATRNLLQLFAAAYLQYSRYLNPNTGEAGSLFDVIDYLATVKRKNDKLRGELY
                     CVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVKLSDDARILAWGNGKEAIVRFAE
                     QHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMGIYFNAEAPSRLEHILQNQNFDDQ
                     DFQTALKLQKMLTENHISKYNVGNSDFTAPSTDKTVILVPGQVEDDASIRYGSPQIYH
                     NLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISPEDAARYADQTAEQADILTCLQY
                     ADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLTQDLLPIPRRSRRLELWQLIAGT
                     LIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNNGLHRGCFAKKLGKIKQLYRSFK
                     "
     gene            8390..9649
                     /locus_tag="ACFNML_000892"
     CDS             8390..9649
                     /locus_tag="ACFNML_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HFC6392301.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELKGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSNSSTPVI"
     gene            10016..11422
                     /locus_tag="ACFNML_000893"
     CDS             10016..11422
                     /locus_tag="ACFNML_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6392302.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     DHLLQNGHPEQALPWLEAAAAQRHPKALFTLLQQREHNGTPTGQLLNEYAWLSEQGHL
                     EAQLILMRYHTQRNDPQSLYWAELAAAQYAAPAYYHLARHHQRQGDVATAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEQAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQAKLAQYALTGELSERDPFQAARYAKAAAEKDHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAGFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAIGNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            11640..12854
                     /gene="gltS"
                     /locus_tag="ACFNML_000894"
     CDS             11640..12854
                     /gene="gltS"
                     /locus_tag="ACFNML_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFC6392303.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            12953..13969
                     /locus_tag="ACFNML_000895"
     CDS             12953..13969
                     /locus_tag="ACFNML_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6392304.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAKANSPLLYGETALSDIVRQKTGGNVEFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDAGVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            13980..14177
                     /locus_tag="ACFNML_000896"
     CDS             13980..14177
                     /locus_tag="ACFNML_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392305.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            14195..14344
                     /locus_tag="ACFNML_000897"
     CDS             14195..14344
                     /locus_tag="ACFNML_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392306.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFLCVSYADVPTHRTIQKAS
                     PYVPS"
BASE COUNT         3830 a         3618 c         3325 g         3619 t
ORIGIN      
        1 ttaatatcta atatttaaaa tacaatgaat aaaaaacgag tcctattaat tgctagttat
       61 gattcttttt tgaatactgg atatgctgtt gcaaaagaaa ttaactattc aacaatcgat
      121 atttatatcc accaaacaaa aaaaaatatt ctttcggaat cacaattaag tttaaatggg
      181 ctttctttaa aagaaatcaa tttttttcat attgatgatt atttcgacaa attaatgtac
      241 cagaattatg atattgttat tctatcatta ggtaatggtt tactgacgcg tttttttcgg
      301 aatagtagca aacttaattt aacattacct atagtaatta ctctatttcc aggggtaatt
      361 tttggtgatt cagaaagtat tataactagg ttaggtgcta atattttatt gttcaataat
      421 tattatgatt accaaatagg caataagttc aaagaacgtt acaacttatc gtgtacaaat
      481 attttatatg gttatcctat cctacgaaaa aaacaccatt gctctgatgg caataaagtt
      541 tatttcattg atcaagtaaa aattccagat ggaaaaaaag aaagattatt aattctaaaa
      601 aaattaatag cactggctaa tcgctatcca cataaagatt tagtactatt attacgtgtc
      661 catcaaaatg atatcacagt acatgaagat aaatactctt atgctgaatt agcaaaaaat
      721 atgaaattgc ctgataattt taaaatagag agaaaacgga cacaagatgc cttacaagaa
      781 atgggatatt gtttatcatt ctcttcaaca atgttatttg aagctgaaag ctgcggaatt
      841 cctattggtg tcattagtga tttaggggtt gacaagaaat atgccaatac acatttcaaa
      901 gatagtggtg ttttggttaa tttagatacg gtagacttgg actctccaaa taccattaat
      961 ccaacttggg ctgaactata tactaatcca gatagcctct cttcaaaaga gtttaaccaa
     1021 ttattagagg ttacttctac tcaaccacct agttttgata attattttat tgcagttaat
     1081 gacgttcagc ctgtatctat ggttctgtta cgtaaattta aaaagcttat atatcatcct
     1141 aagcaattct ttgttgattc aaaactatat aaattaatta aatttactaa ataattttta
     1201 ctatgggttg ccttatttat ttcaaaggag tgacataaaa aatgcaaaaa attcgtaaag
     1261 ctgtattccc tgtagctggc atgggaaccc gattcttacc tgccacgaaa gccagcgcta
     1321 aagaaatgct gccaatcgtg gacaaaccgt taattcaata tgctgcggaa gaagccgttg
     1381 ccgcgggttg tactgaattg atttttatca ccggccgtaa taagcgtaat attgaagacc
     1441 acttcgataa agcctatgag ttggaaactg aactggaaat gcgtgaaaag cataaattgc
     1501 ttggctatgt gcaaaaaatt ctgcctgatc atgtcagctg tattttcatt cgtcaggcag
     1561 aagctttggg cttgggtcat gcggtattat gcgcccgtcc tgctgtcggt aatgagcctt
     1621 ttgccgtgat tttggccgat gacttgattg atgcaccaca aggcgcactc aagcagatgg
     1681 tagatattta caatcaaagc ggcaacagca ttttaggggt agaaacagtc gatccttcgc
     1741 aaaccggttc atacggcatt gtcgaagtgg aacagctgaa aagctatcag cgtgtaacca
     1801 atattgtgga aaaaccaaag cctgaagatg cgccgtcaaa tttagcggta gtcggccgtt
     1861 atattttgac accgcgtatt tttgatttgc tgactaatct acctcgtggt gcaggtggtg
     1921 aaatccagct aactgatggt attgcccgcc tacttgacca tgaatttgtc ttggcgcatg
     1981 cgtttgaagg taagcgttat gattgtggca gtaagttggg ctatctggaa gccacggtag
     2041 cttatggctt gaagcatccc gaattaaaag atgatttcaa agaattattg aagcaatttg
     2101 tataatttta aggccgtctg aaaaagaaaa ggcatcatgt tttttacact ttcaggcggc
     2161 cttttaaaca taaagaagga aaacgtgatg aacattctcg ttaccggcgg caccggtttt
     2221 atcggctcgc acaccgttgt ttctttgctg aaaagcggtc atcaagtcgt gattttggat
     2281 aacctatgca attccagcat caatatcctg ccacgcttga aaacgattac cggccaagag
     2341 attccgtttt atcaaggcga tatccgcgat cgtgagattt tgcgccgtat ttttgcggaa
     2401 aaccgcattg attcggtgat tcattttgcc ggcttgaaag cggtgggtga aagtgtggcc
     2461 gagccgataa aatattatga taataatgtt tccggcagct tggtgttggc ggaagaaatg
     2521 gcgcgtgcgg gcgtgtttag cattgtgttc agttcttcgg cgacggttta tggcgatccg
     2581 ggcaaagtgc cttataccga ggatatgcca ccgggcgaca ccaccagccc ttacggcgca
     2641 tcgaaatcga tggttgagcg cattctcact gacattcaaa aagccgatcc gcgctggagc
     2701 atgattttgt tgcgttattt caatccgatt ggcgcgcatg aaagcggttt gattggcgag
     2761 cagccaaacg gtatcccaaa taatttgttg ccttatatct gccaagtggc ggcaggcaaa
     2821 ctgccgcaat tagcggtatt cggtgatgac taccctaccc ctgatggcac gggtatgcgc
     2881 gactatattc atgtgatgga tttggcagaa ggccatgtcg cggctatgca ggcgaaaagc
     2941 aatgtagcag gcacgcattt gttgaactta ggttccggcc gcgcttcttc ggtattggaa
     3001 atcatccgcg catttgaagc ggcttcgggt ttaaccattc catttgaaat caaaccgcgc
     3061 cgcgccggtg atttggcgtg cttctatgcc gacccttctt atacgaaggc gcaaatcggc
     3121 tggcaaacgc agcgtgattt aacccaaatg atggaagatt catggcgctg ggtaagtaat
     3181 catccgaatg gttatgatga ttaagtcatg aaattgagag gccgtctgaa gtttcagacg
     3241 gcctttgttt gcgatttata gtaaaattta ccttatcatt ttggcgatta atattcatca
     3301 tgaaaaaaat ccttgtcacc ggcggtgccg gttttatcgg ttcggcggtt gtacgccata
     3361 tcatcaacga tacgcaagat gcggtggtga atgtggataa gctcacgtat gcgggcaatt
     3421 tggaatcgct ggaatctttg gccaatcatc cgcgctatgc gtttgagcaa gtcgatattt
     3481 gcgaccgcgc ggcactggat cgtgtgtttg ccgaacacca gccggatgcg gtgatgcact
     3541 tggcagcaga aagccatgtt gaccgcagta ttgatggccc agcggcattt attgaaacca
     3601 atattgttgg tacttacacg cttttggaag cggcgcgcgc gtattggcat gatatgccgt
     3661 ctgaaaaaaa agcggctttc cgtttccatc atatttcgac tgatgaagtg tatggcgatt
     3721 tgcacggcac ggatgatttg tttaccgaaa ccacgcctta cgccccttcc agtccttatt
     3781 cggcttccaa agcctccagc gaccatttgg tgcgcgcatg gctgcgtact tacggcctgc
     3841 cgaccattgt gaccaattgt tctaataact acggccctta tcattttcct gaaaaactga
     3901 ttccactgat gattctgaat gcgcttgatg gcaaaccgct gccggtgtat ggcgacggta
     3961 tgcagattcg tgattggctg tttgttgaag atcatgcgcg tgcgctgtat caagtggtca
     4021 cagaaggcga aatcggcgaa acttacaaca ttggtggcca caatgaaaaa gccaatattg
     4081 aagtggtgcg caccatttgc gccctgctgg aagaattggt gccgaacaaa ccgcaaggcg
     4141 tggcgcgtta tgaagatttg attacctatg tcaaagaccg ccccggccat gatgtgcgct
     4201 atgcgattga tgccgcaaaa atcggccgtg agttgggttg gcagccgcaa gaaacttttg
     4261 aaagcggcat ccgcaaaaca gtggaatggt atttaaacaa tcaaacttgg tggtcgcgcg
     4321 tgttagacgg cagctacaat cgtgaacgct taggcagtta aatagagacc tttgcaaact
     4381 ccccatcttt ggcacatttc ttcgttatgc gctgctcgaa agcttgccta tctttgtgat
     4441 atgtctgcgc tttctgcgct gctataactt cgaactgtac tcaaatctgt ggctttgcaa
     4501 aggcctcaaa taataaaaat acgttcagac ggcatgaagg ccgcctgaaa aactacataa
     4561 ggaaagtaaa ctgatgaaag gcattattct cgcaggcggc agcggtatcc gcctttaccc
     4621 gattacccgc ggcgtatcaa agcaattact accggtgtac aacaaaccga tgatttatta
     4681 ccccttgtcg gtgttgatgt tggccggaat ccgcgatgtg ctggtgatta ccacccccga
     4741 agacaacgat gctttccgcc gcttactggg tgatggcagc gatttcggta ttcaaatttc
     4801 ctacgccatc caacccagcc ccgatggttt ggcgcaagcc tttttaattg gtgaagaatt
     4861 tatcggcgat gattcggtgt gtttggtgtt gggcgacaat attttctatg gccagcattt
     4921 cagccaatcc ctgaaagaag ccgccgcttc cgtggaaacc aaaggtgcta ccgtattcgg
     4981 ctatcaagtg aaagatcccg agcgtttcgg tgtgattgag tttgatgaga atttcaaagc
     5041 cctgtcaatc gaagaaaaac cgaaacaacc taaatccgat tgggcggtaa ccggcctcta
     5101 tttctacgat aatcgcgtag tcgagtttgc caaacaagtg aagccgtctg aacgcggcga
     5161 attggaaatt accaccttga atcaaatgta tttggacgat ggttcgctca atgtccaatt
     5221 gctcggtcgc ggtttcgcat ggcttgatac cggcacacac gccagcctgt tggaagcagc
     5281 ctcgtttgtc gaaaccattg aaaacattca aaacctacaa gtcgcctgtt tggaagaaat
     5341 cgcatggcgt aatggctggt tgagcagcga agaaatcaga aacttggcaa agccaatgtg
     5401 taaaaatgaa tatggccgct atttattgcg cttggttggc aaagattaag aggttgcttt
     5461 taccagtttt caggccgtct gaaacgcttt ttaggaaaac atgatgaata tcatcgatac
     5521 ccgcattccc gatgtcaaat tgctcgaacc caaagtcttc ggtgacgagc gtggtttttt
     5581 tatggaaacc ttccgtgatg aatggttccg ccaaaatgta gccgagcgca ctttcgtgca
     5641 ggaaaaccac agcaaatcca cccaaggcgt attacgcggt ttacattatc aaaccgaaaa
     5701 tacacaaggc aaactggtgc gcgtggtatc aggcgaagtc tttgatgttg ccgtggatat
     5761 gcgtcgtgat agtcctactt tcggccactg ggtcggtgag gttttatcgg cagacaacaa
     5821 acgccaatta tgggtgccgg aagggtttgc ccatggattt tatgtgttga gcgatgaagc
     5881 ggaattcgtc tacaaatgca ccgactatta caacccgaat gcggaacatt ccctaatttg
     5941 gaatgatgcc acagtgggaa tcgactggcc tttattggga gaaccaagcc tatcggccaa
     6001 ggatttggca ggcaagatgc ttgctgaagc ggccacattt taattttcac ttttaggtca
     6061 tcgtgggtaa tttgtagcaa gatgacctaa tttttaccag ctagcctact aactaaattt
     6121 ctactatatt taaatagtca gaaaatttac acaaaatctg ttaataagat attatattgt
     6181 cagtaatatt ttttcctatt tattgtgatt cctgcctaca ttcctcttcg ctctttcggt
     6241 atacgtaaaa ttccgaatat tacgcaattt ctaccggaat attcacttaa caaaaccgta
     6301 ttcaacgccc aaaccatcat tggctggggc ttacgcccga ccacccacca agcacgtgct
     6361 tttgcacaat cacatggctt gccgtttgta gccttggaag acggtttttt acgctcactg
     6421 ggcttaggcg tggccggtta tccgcccttc gcactggttt acgacgatat cggcatctat
     6481 tacgacacaa cccgcccatc gcgtttggag cagttgattt tagccgcaaa catgccgtct
     6541 gaaatgctta accaagcgca gcaggccatt gatttgattt tgacgcaccg tttgtctaaa
     6601 tataaccatg ctctggattt ttcagacggc ctcgtacaag acagcaaaaa cattgttttg
     6661 gtaattgatc aaactttcgg cgatatggcg attcaatatg gcggcgcaga tgaagccaca
     6721 ttccgccaaa tgtttcagac ggccttagcc gaaaatcctg atgcggaaat ctgggtcaaa
     6781 acccatcccg atgtattgag cggcaagaaa cagggttacc ttactgattt ggcacagcag
     6841 ccgcgtgtgc gtttattggc tgaagacatt aatccgattt cgctgctgca agcggtggac
     6901 aaagtgtatt gcgtgacctc gcaaatgggt tttgaagcct tgttgtgcgg caagcctttg
     6961 gtaacgtttg gtttgccgtg gtatgccggc tggggtgtaa gcgacgaccg ccatcctgaa
     7021 atcaaccgcc ttgttcaaac ccaacgccgc gccacccgca acttgctgca gctcttcgcc
     7081 gcagcctatc tgcaatacag ccgctacctc aaccccaata ccggcgaagc aggcagcctc
     7141 tttgatgtca tcgactatct ggcgacggtc aaacgtaaaa acgacaaatt gcgtggcgag
     7201 ttatattgcg tcggtatgtc tttgtggaaa cgcgcggttg ccaaaccgtt ctttaacgta
     7261 ccctcttgcc gtctgaaatt tatctcttcc acccaaaaac tggcaagggt caaactgtcc
     7321 gacgatgcac gcatcctggc ttggggcaac ggcaaagagg ccatcgtccg ctttgccgaa
     7381 caacaccaca tccccctgct gcgcatggaa gacggcttta tccgctcggt cggactcggc
     7441 tccaacttag tgccgccgct gtcgctcgtt accgacgata tgggtattta cttcaatgcc
     7501 gaagccccat cccgccttga acacatcctg caaaaccaaa acttcgacga tcaagacttt
     7561 cagacggcct tgaagttgca aaaaatgctg accgaaaacc atatcagcaa atacaacgtc
     7621 ggcaactcag actttaccgc cccatcaacc gacaaaaccg tgatcctcgt tcccggtcag
     7681 gttgaggatg atgcgtctat ccgctatggt tcgccccaaa tctatcacaa tctggatttg
     7741 ctccgtaccg tacgcgaacg aaaccccaat gcctatatca tctacaaacc gcatcccgat
     7801 gtagtcagcg gtaaccgcat cggccatatt tcccctgaag atgctgcacg atatgccgac
     7861 caaaccgccg aacaagccga catcctgacc tgtctccaat acgcagacga aatacatacc
     7921 atgacttcgc tgaccggttt tgaagccttg ttgcgcggca aaaaagtcag ctgctacggc
     7981 ctgccttttt acgcaggctg ggggcttacc caagatctgc tccccatccc gcgccgtagc
     8041 cgcagacttg agctttggca gctgattgcc ggcacgctca tccactatcc cgactacatc
     8101 caccccgaaa cccatcaggc cataaatgcg gaaaccgcag cccaaatcct gatacgacaa
     8161 aaaaatatgc aaaaaaacaa caacggatta catcgcgggt gctttgccaa aaaattaggt
     8221 aaaatcaaac aactatatcg atctttcaaa taaataccat caaagttaac gatgcgtcat
     8281 aaacttgcct ctattgcggc atcattgcct ttgcatcgtt aattctcttg gcgtatgctt
     8341 gaaagttcaa cctaaaacta ttacataaaa aacaaaacca cattgcaaca tgaaacagac
     8401 cgtcctcaaa aataacctgc aaaacctgct tgaaagcgca gaaaatatcc tgctgcttca
     8461 aggccctgtc ggcgattttt ttctgcgcct tgccgactgg ctgactgcaa acggcaaaac
     8521 cgtacataaa ttcaacttta atgcaggcga cgactatttt tatccgccca ctcaagcgca
     8581 taccgttgtt tttaacgaca actacgatgc ctttcctgag tttttgcaag aatacatcac
     8641 tcaacatcac atccaggccg ttgtctgctt tggcgacaca cgcccttatc acgtcattgc
     8701 aaaacgcatt gcaaacgaaa accaagccag tttctgggcg tttgaagaag gctatttccg
     8761 cccctactac atcaccttag aaaaagacgg cgtcaacgca ttttccccgt tgccgcgccg
     8821 tgccgacttt tttcttgaac aattccctaa gcttgcccag caagaatata aagcgccaac
     8881 gccggtacac ggcggtttta cgcccatggc aaaaaacgct atccgttact atatcgagtt
     8941 gttccgcaat ccacgcaaat accccgacta catccaccac cgcgcaccca atgccggcca
     9001 ttacctcaaa ccgtggtcgc tctccatcct caagcgtttg aactactata ttgaagacat
     9061 ccaaatcgcc aaacgtgtgg aagcaggcaa atacggcaag ttttttattg ttcccttaca
     9121 ggtattcaac gacagccaag tccgtatcca ttgcgacttt cccagcgtcc gcagcttcct
     9181 gctccatgtt ttgagttcat ttgccgagca cgcgcctgcc gataccaaca tcatcatcaa
     9241 gcatcatccg atggaccgcg gttttatcga ctactggcgc gacattaaac gctttatcaa
     9301 agaacacccc gaactcaaag gccgcgtgat ttatgtccat gatgtccccc tgcccgtttt
     9361 cctgcgccac ggtctcggca tggtcaccat caacagcacc agcggcctgt ccggactgat
     9421 tcacaatatg ccagttaagg ttctcggccg tgcctattat gatattcccg gcattactga
     9481 ccaaaatacc ttggcagaat tttggaatca tccgacaccg cctgacaaag agctgttcca
     9541 tgcctaccga atgtaccacc tcaacgtgac ccaaattaac ggcaacttct acagtcaggt
     9601 gtttttcccc aacaaaaaaa cctccaactc ttccacacca gtaatctgac ttagcgaagg
     9661 aagttcaggc cgtctgaaaa catttcagac ggcctgaaac aatcaatacc ttagctactg
     9721 ccatgtaaat aaaacacaaa aatctgcatt tatcattaac aataaattac aaaaacagta
     9781 taatgaccga gctgccatga gcgcataccg actcaacctg agccctttat aacacacaaa
     9841 atatggatgt atccccaggc aaaacaatat aacaagccaa acatcctaaa gataagccgg
     9901 caaggaaata cactctgcaa aacgatgccg agcaatattt ttacaaagcc ctcaaccggt
     9961 atcgccgccc atatgccgca gcatccgtct tccactttat atccgcccgc aaaccatgac
    10021 cgccgctcct gatatcctat accgtcaagc cgccgccctt ttggaacaat ccaataccgc
    10081 ccaagccctg cccctgctgc aacaggccgc agagcaggga tatacggaag ctgcttttgt
    10141 attgggcgac catctgctgc aaaacggtca tccggagcag gcacttccat ggttggaagc
    10201 cgccgcggcc caacgccatc ccaaggcact ctttaccctg ctgcaacaac gcgaacacaa
    10261 cggcaccccg accggacagc ttcttaacga atacgcctgg ctgagtgagc aaggtcacct
    10321 cgaagcccaa ttaattctca tgcgttacca cacgcagcgc aacgatccac aatcgctcta
    10381 ctgggcggag cttgccgctg cccaatatgc cgcacctgcg tattaccatt tggcacgcca
    10441 tcatcaacgc caaggcgacg ttgcaacagc tatcgaacaa tacgaaaaag cggcagcgct
    10501 cggcgtgact gcagcctgct ggcaactcgg tcaaatctac ttctacggca caggtgtcag
    10561 ccccaaccac gcacaagccg aacactatct cgaacaagcc gcgcaagccg gccacatcgc
    10621 cgcacaaacc ctgctggccg accttcttgc tgcccaacgc aaacccgaag ccttagaatg
    10681 gtatcgtcgt gccgccgaca aagagcaagc ggaagcacag gccaagctgg ctcaatacgc
    10741 cctgaccggc gaactttccg aacgcgatcc gtttcaagcg gcacgatatg ccaaagccgc
    10801 cgctgagaaa gaccatcccg aagccctgaa aatcatgggc gacctctacc gctacggcct
    10861 cggcatcaaa gctgacaacc atatcgcgca cgattactac caccgtgctg ccgcgctggg
    10921 ttctgccgcc gcagcacaaa aactcatcag cgacgccgcg ctgtaccatc cgcaacaata
    10981 cgaacaaatc aaaactgccg ccctgcaaca gcaacaaacc gaaaccatct accgtttggc
    11041 ggaaacacaa gcccatgcca tcggtcgtcc cgccgactat aatgccgcac gcaaaaatta
    11101 catggaagct gccgggttcc accataaaaa cgcggcggct gccttaggcc gcatctacca
    11161 ttacggcctc ggtacggcgc aagatcctcg ggcggctgca cactggtacg ccattgctgc
    11221 cgaacaaaac cacccttccg cccaatacca cctcgcctgt ttttactatc acgggcaagg
    11281 tgtcggctgt catgttccga ccgcctgcta ctggctgcag gccgccatcg gcaacggcca
    11341 cacttcggcc gaatcattaa tatccctatt agaacaatgg cgacgcgaag cacaccatgc
    11401 catcggacaa aaggccgtct gaaaagattt acactcgcat tttttgacaa tctttaacta
    11461 ttcccctaat atttgccagt tatttttcac ggacacgcca ttgttttcat ttctttctga
    11521 aaacaccttg tccgcgcatc aataccatga cactcggcgg ataacgccaa gcgttgaaac
    11581 acactacatc cggaacaaaa acggatgctc ggaaaaatat ttctaggagg tgaaacaaca
    11641 tggaatggga attcaacagt tattacacac tgattgccgc cacgctcgtg ttgctggttg
    11701 gtaaatttct ggttcaaaaa atcaaattct tacgagactt caatattccc gagccggtcg
    11761 ctggcggttt ggttgccgcg attatcctgt ttgcgctgca cgaggcgtac ggcgtgagct
    11821 tcaaatttga gaaaccgctg caaaatgcgt ttatgctgat ttttttcacg tccatcggct
    11881 tgagcgcgga tttttcccgt ttgaaggcgg gcggtttgcc gctggtggtt tttaccgcga
    11941 ttgtgggcgg atttatcttg gtgcaaaact ttgtcggggt cggactggct acggctttgg
    12001 gtttggaccc gctcatcggt ctgattaccg gttcggtgtc gctgacgggc ggacacggta
    12061 cgtcaggtgc gtggggacct aattttgaaa cgcaatatgg cttggtcggc gcaaccggtt
    12121 tgggtattgc atcggctact ttcgggctgg tgttcggcgg cctgatcggc gggccggttg
    12181 cgcgccgcct gatcaacaaa atgggccgca aaccggttga aaacaaaaaa caggatcagg
    12241 acgacaacgc ggacgacgtg ttcgagcagg caaaacgcac ccgcctgatt acggcggaat
    12301 ctgccgttga aacgcttgcc atgtttgccg cgtgtttggc gtttgccgag attatggacg
    12361 gcttcgacaa agaatacctg ttcgacctgc ccaaattcgt gtggtgtctg tttggcggcg
    12421 tggtcatccg caacatcctc actgccgcat tcaaggtcaa tatgttcgac cgcgccatcg
    12481 atgtgttcgg caatgcttcg ctttcgcttt tcttggcaat ggcgttgctg aatttgaaac
    12541 tgtgggagct gaccggtttg gcggggcctg taaccgtgat tcttgccgta caaaccgtgg
    12601 tgatggtttt gtacgcgact tttgttacct atgtctttat ggggcgcgat tatgatgcgg
    12661 cagtattggc tgccggccat tgcggtttcg gcttgggtgc aacgccgacg gcggtggcaa
    12721 atatgcagtc cgtcacgcat actttcggcg cgtcgcataa ggcgtttttg attgtgccta
    12781 tggtcggcgc gttcttcgtc gatttgatta atgccgcgat tctcaccggt tttgtgaatt
    12841 tctttaaagg ctgattttcc gcctttccgc caaagcacct gcaaggttta ccgcctgcag
    12901 gtgcttttgc tatgatagcc gctatcggtc tgcaccgttt ggaaggaaca tcatgtatcg
    12961 gaaactcatt gcgctgccgt ttgccctgct gcttgccgct tgcggcaggg aagaaccgcc
    13021 caaggcattg gaatgcgcca accccgccgt gttgcaaggc atacgcggca atattcagga
    13081 aacgctcacg caggaagcgc gttctttcgc gcgcgaagac ggcaggcagt ttgtcgatgc
    13141 cgacaaaatt atcgccgccg cctacggttt ggcgttttct ttggaacacg cttcggaaac
    13201 gcaggaaggc gggcgcacgt tctgtatcgc cgatttgaac attaccgtgc cgtctgaaac
    13261 gcttgccgat gccaaggcaa acagccccct gctgtacggg gaaaccgctt tgtcggatat
    13321 tgtgcggcag aagacgggcg gcaatgtcga gtttaaagac ggcgtattga cggcagccgt
    13381 ccgcttcctg cccgtcaaag acggtcagac ggcatttgtc gacaacacgg tcggtatggc
    13441 ggcgcaaacg ctgtctgccg cgctgctgcc ttacggcgtg aagagcatcg tgatgataga
    13501 cggcaaggcg gtgaaaaaag aagacgcggt caggattttg agcggaaaag cccgtgaaga
    13561 agaaccgtcc aaacccacgc ccgaagacat tttggaacac aatgccgccg gcggcgatgc
    13621 gggcgtaccc caagccgcag aaggcgcgcc cgaaccggaa atcctgcatc ctgacgacgg
    13681 cgagcgtgcc gataccgtta ccgtatcacg gggcgaagtg gaagaggcgc gcgtacaaaa
    13741 ccagcgtgcg gaatccgaaa ttaccaaact ttggggagga ctcgataccg acgtgcaaaa
    13801 agagttggtc ggcgaacaac gcaagtgggc gcaggaaaaa atcagcaact gccgacaagc
    13861 cgccgcgcag gcagaccggc aggaatacgc cgaatacctc aagctgcaat gcgacacgcg
    13921 gatgacgcgc gaacggatac agtatcttcg cggctattcc atcgattagg ggcaaaccga
    13981 tgactaccgt ccccaaaagc aggattcccg tcaaaccgct gcccgaaaaa accacagacg
    14041 aagccaaagt cgaaaaatgg cggcagctcg gtgcggaaca cggtttgtcg ggcgaatggg
    14101 cagttgccgc cagattgggc gagaacggtt ttaccgaaga acagatggaa aatatcgcca
    14161 acctgttcgg cagataaagg gaaaattgac ggaaatgccg tctgaaaccc tgttatcggt
    14221 ttcagacggc attttgacta atacggtacg caggcgcaaa acagccggct ttttatgtgt
    14281 ttcctatgct gatgttccaa cacacaggac gatacaaaaa gcgtcgccct atgtgccgtc
    14341 ctgattcgga aagggttacg ctcctcccaa atatagtgga ttaacaaaaa cc
//
LOCUS       DAXHTR010000045        13838 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.045, whole genome shotgun sequence.
ACCESSION   DAXHTR010000045 DAXHTR010000000
VERSION     DAXHTR010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13838
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.045"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(122..1447)
                     /locus_tag="ACFNML_000898"
     CDS             complement(122..1447)
                     /locus_tag="ACFNML_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFC6392307.1"
                     /translation="MKKYLFRAALCGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPAPAGTTVAGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQVAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGRYMADKGYLKLGQTSMQGIKAYMQQNPQRLAEVLGQNPSYIFF
                     RELTGSSNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            1659..2498
                     /gene="efeU"
                     /locus_tag="ACFNML_000899"
     CDS             1659..2498
                     /gene="efeU"
                     /locus_tag="ACFNML_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFC6392308.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGIGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGVLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIVVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVMTWFLCGSRPSETLTRKE
                     ELK"
     gene            2495..3661
                     /gene="efeO"
                     /locus_tag="ACFNML_000900"
     CDS             2495..3661
                     /gene="efeO"
                     /locus_tag="ACFNML_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFC6392309.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASGEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRIHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIEAKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            3925..5190
                     /gene="efeB"
                     /locus_tag="ACFNML_000901"
     CDS             3925..5190
                     /gene="efeB"
                     /locus_tag="ACFNML_000901"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFC6392310.1"
                     /translation="MSKNQPAQPTRRTLFKTAIAAGAVGAIGGYLGGKKRGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPIHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPEGNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYSYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(5262..5591)
                     /locus_tag="ACFNML_000902"
     CDS             complement(5262..5591)
                     /locus_tag="ACFNML_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFC6392311.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(5668..7038)
                     /gene="glmU"
                     /locus_tag="ACFNML_000903"
     CDS             complement(5668..7038)
                     /gene="glmU"
                     /locus_tag="ACFNML_000903"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFC6392312.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMLGRVIDTAA
                     ALNPQNICVVIGHGKDQVLNAVKRDVVWVEQTEQLGTGHAVKTALPHLAAEGRTLVLY
                     GDVPLIDVETLETLLEAAGNEVGLLTDVPADPSGLGRIIRDGSGSVTAIVEEKDADAA
                     QKAVKEINTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SHLAAGVNNKRQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDVN
                     CIFEGEVELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLAADVHVGNFVEIKNAAIGKGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVAPVTLGNKVTTGAGSTITRNIEDNKLALARARQTVIEGWMR
                     PEKDKQ"
     gene            complement(7093..7365)
                     /locus_tag="ACFNML_000904"
     CDS             complement(7093..7365)
                     /locus_tag="ACFNML_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HFC6392313.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPIYY
                     QDGSYSKNMNYNQYRPERHAVLPNQTGNNADEEHRQHWQKPKFQNR"
     gene            complement(7341..8072)
                     /locus_tag="ACFNML_000905"
     CDS             complement(7341..8072)
                     /locus_tag="ACFNML_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFC6392314.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGLFTLINRAMTRYMTRRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPETQNTLSR
                     LKGRKAVFSNGPSFYVRAVVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLARYAETLSEH
                     RQNHYNTRTTPRKYERMLCVKPSLS"
     gene            8131..9132
                     /locus_tag="ACFNML_000906"
     CDS             8131..9132
                     /locus_tag="ACFNML_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6392315.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGLGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPELAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            9226..10074
                     /locus_tag="ACFNML_000907"
     CDS             9226..10074
                     /locus_tag="ACFNML_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HFC6392316.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            10100..10585
                     /locus_tag="ACFNML_000908"
     CDS             10100..10585
                     /locus_tag="ACFNML_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HFC6392317.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(10687..12255)
                     /gene="msrAB"
                     /locus_tag="ACFNML_000909"
     CDS             complement(10687..12255)
                     /gene="msrAB"
                     /locus_tag="ACFNML_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HFC6392318.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            12401..13666
                     /gene="ftsY"
                     /locus_tag="ACFNML_000910"
     CDS             12401..13666
                     /gene="ftsY"
                     /locus_tag="ACFNML_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFC6392319.1"
                     /translation="MFSFFRRKKKQETPAPEEAQVQETAAKAESEVTQIVENIKEEVE
                     SLTESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         2981 a         3552 c         4137 g         3168 t
ORIGIN      
        1 aatttgatcc actataattc cgtcaaataa gaaaggaatt ttgtgcctgc ggtatcgcaa
       61 aacttcgcct taatgcgccc gattgcctag ggatgggctt cagacggcat tgttttccgg
      121 tttacgggcg gtattcgggc ttcataccgt tgggcagaag ctgccagaca tatcccgtgg
      181 ttttctgttt gccggcaagt tcgccggctt cgtcgccgta accccaaaaa taatccacgc
      241 gcaccgcgcc tttaatcgcg ctgccggtat cctgcgccat aatcaggcgg ttgagggctt
      301 tgcgggtaac cggatgggcg gtggcgacaa ataagggcgc gcccaaggta atgtagtgcc
      361 ggtcgactgc gccggcgtac tcgcccatca gcggcgtgcc cagtgcgccg acagggccgt
      421 cattgctgct tccggtaagc tctcggaaaa agatatagct ggggttttgc cccaaaactt
      481 cggcgaggcg ttgcgggttt tgctgcatat aggctttgat gccctgcatc gaggtctgcc
      541 cgagcttgag gtagcctttg tccgccatat agcgtccgat ggaaacgtag ggatgttcgt
      601 ttttgtcggc atagccgatg cggatgtatt tgccggacgg ggttttcaga cggcccgagc
      661 cttggatgtg cataaaaaaa agttcgacgg ggtcttcggc gtaaccgagt atcggggctt
      721 tgccgtcaag cgcgccgccg ttgatttggt tgcgcgtgtg gtaggggagg aagcggcttc
      781 cttcaaacct gcctttgatt gccgttgtgc gcgcggtgat ggggaatcgg gagaggtcgg
      841 cggtgtgcgt gccgccggca ttgtcgattg tgccgctgtt tttccccgtc tgcctgatgc
      901 ggacaagggc ttttccgctc cgcaaaccgg caggcagggg gacggagata aaatcgtcgg
      961 gaataccgta aatcgggaag cgggcttgtg ccgtccgcct gtcgtcgccc ttcagcaccg
     1021 gctcgtaata gccggtaacc gtaccggcaa ggcttccgtt gcctgcaacc tgccacggcg
     1081 tgaaatagcg ttcaaaaaac tgttttgcct gaaaggaatg gacgggggtt tgaaaggctt
     1141 gggcacacac atcctgccag ccttggcggt ttttcaaatt ggcgcagccg aggcggaagg
     1201 attgcaggct tttggcgaaa tcctgcgccg cccagtgggg cagggacagg tgcggcacaa
     1261 cggtataaac ggccccgccg ccggcaaccg tcgttccggc gggggcgggg atgccgaccg
     1321 gccggtccgg gccgttgatg acggatgtgt cgggttgcgg aaaggtttgg atgctcttgc
     1381 tttggcaggc ggcgaggatg gcggcggcga tgccgcacag ggcggcgcgg aacaggtatt
     1441 ttttcataat ggacaatgtt gtcagcagta ataaaaaaag atggtttcgg gcggcgttgc
     1501 ggcagccgtg gagaggggat tttaacacag ggcgcagctg cagcctgcgg aactttccgc
     1561 cgcgcggtac tgcagataaa aataacttgc atttgtattt acaagcaatg aaaatattcc
     1621 gataatatat tattcatcat ccttgttcgt tcgcgtttat gctggtcgct tttttaatta
     1681 tgttgcgcga gggtatcgaa gccgcgctca tcgtcggcat cgttgccggt tttctgaaac
     1741 agtccggaca ttccaaactg atgcctaagg tctggttcgg ggtcgtcctt gcttctttga
     1801 tgtgtttggg catcggatac ggcatccatt cggcaacggg cgagattccc cagaagcagc
     1861 aggagttcgt cgtcggcatt atcggtttgg ttgctgtcgc gatgctgact tatatgattt
     1921 tatggatgaa aaaggcggcg cgttcgatga agcagcagct tcaggattct gtacaggcgg
     1981 ctttgaaccg tggcagcggt caaggatggg ccttggtcgg tatggcgttt cttgccgtgg
     2041 cgcgtgaagg tctggagagt gtttttttcc tgcttgccgt attcaaacag agcccgactt
     2101 ggcagatgcc ggccggcgcg gtagcggggg ttttggctgc cgccgtgatt ggcgcgttga
     2161 tttatcaggg tgggatgcgc ctgaatctgg cgaagttttt ccgttggacg ggggcgtttc
     2221 tgattgtcgt tgccgccggc ctgcttgccg gttcgctgcg cgcgctgcat gaggcaggta
     2281 tttggaacgc gcttcaggac attgtgttcg actcatcaaa atatttgcac gaagacagcc
     2341 cgttgggcgt gctgctcggc ggatttttcg gctataccga ccatccgacg cagggcgaag
     2401 ccttggtttg gctgctgtac cttattcccg tcatgacttg gtttttgtgc ggcagcaggc
     2461 cgtctgaaac tttaacccgt aaagaggagc tgaaatgaga aaattcaatt tgaccgcatt
     2521 gtccgtgatg cttgccttag gtttgaccgc gtgccagccg ccggaggcgg agaaagctgc
     2581 gccggcagcg tccggtgagg cgcaaaccgc caacgagggc ggttcggtca gtatcgccgt
     2641 caacgacaat gcctgcgaac cgatggaact gaccgtgccg agcggacagg ttgtgttcaa
     2701 tattaaaaac aacagcggcc gcaagctcga atgggaaatc ctgaaaggcg tgatggtggt
     2761 ggacgagcgc gaaaacatcg cccccggact ttccgataaa atgaccgtca ccctgttgcc
     2821 gggcgaatac gaaatgactt gcggtctttt gaccaatccg cgcggcaagc tggtggtaac
     2881 cgacagcggc tttaaagaca ccgccaacga agcggatttg gaaaaactgt cccaaccgct
     2941 cgccgactat aaagcctacg ttcaaggcga ggttaaagag ctggtggcga aaaccaaaac
     3001 ctttaccgaa gccgtcaaag caggcgacat tgaaaaggcg aaatccctgt ttgccgacac
     3061 ccgcatccat tacgaacgca tcgaaccgat tgccgagctt ttcagcgaac tcgaccccgt
     3121 catcgatgcg cgtgaagacg acttcaaaga cggcgcgaaa gatgccggat ttaccggctt
     3181 ccatcgtatc gaatacgccc tttgggtgga aaaagacgtg tccggcgtga aggaaattgc
     3241 agcgaaactg atgaccgatg tcgaagccct gcaaaaagaa atcgacgcat tggcgtttcc
     3301 tccgggcaag gtggtcggcg gcgcgtccga actgattgaa gaagtggcgg gcagtaaaat
     3361 cagcggcgaa gaagaccggt acagccacac cgatttgagc gatttccaag ccaatgtgga
     3421 cggttcgaaa aaaatcgtcg atttgttccg tccgctgatc gaggccaaaa acaaagcctt
     3481 gttggaaaaa accgatacca acttcaaaca ggtcaacgaa attctggcga aataccggac
     3541 taaagacggt tttgaaacct acgacaagct gggcgaagcc gaccgcaaag cgttacaggc
     3601 ctctattaac gcgcttgccg aagaccttgc ccaacttcgc ggcatactcg gcttgaaata
     3661 agccgcaagc gttcagacgg tatttggcgg cagataccgt ctgaagtttt atagtggatt
     3721 aacaaaaacc agtacggcat tgcctcgcct tgccttgccg tactatttat actgtctgcg
     3781 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ccgccatata ttgcagggcg
     3841 ggatttcaac ctgccgctat cggttaatgg aaaaacggcg tgcagggata cccatcctgc
     3901 tgcacggata ttgaaggaaa caccatgagc aaaaaccaac ccgcacaacc gaccaggcgc
     3961 actcttttta aaaccgcgat cgcagccgga gcagtcggcg caatcggagg ttatctcggc
     4021 ggcaaaaaac ggggcgaaac cgccgaacgc accgccgaaa gtcaacactc gccccaagcc
     4081 tatccctgct acggcgaaca tcaggcaggc atcgttacgc cgcagcaggc gttttcgatt
     4141 atgtgcgcct tcgacgtaac cgcgcaaagt gccaagcagc tggaaaacct gttccgcacg
     4201 ctgaccgccc gcatcgagtt tctcacccaa ggcggcgaat accaagacgg cgacgacaaa
     4261 cttccgccag ccggcagcgg cattttgggc aaagccttca accccgacgg gttgaccgtt
     4321 accgtggggg tgggcagcag cctgtttgac ggccggttcg gactcaaaga caaaaaaccg
     4381 attcatttgc aggaaatgcg cgacttctcc aacgataagc tgcaaaaaag ctggtgcgac
     4441 ggcgatttga gcctgcaaat ctgtgccttc acccccgaaa cctgccaagc cgccctgcgc
     4501 gacatcatca aacacaccgt ccaaaccgcc gttatccgct ggagtatcga cgggtggcag
     4561 cctaaatccg aacccggcgc gatggcggcg cgcaacctgt tgggcttccg cgacggcacg
     4621 ggcaacccca aagtttccga ccccaaaacc gccgacgagg ttttgtggac gggtgtggcc
     4681 gccaacagcc tcgacgaacc ggagtgggcg aaaaacggca gctatcaggc agtccgcctt
     4741 atccgccact ttgtcgagtt ttgggacagg acgccgcttc aagagcaaac cgacattttc
     4801 gggcggcgca aatacagcgg tgcgccgatg gacggcaaaa aagaagccga ccaaccggat
     4861 tttgccaaag accccgaggg gaataccacg cccaaagaca gccatatacg cctggcgaat
     4921 ccgcgcgatc ccgaattcct caaaaaacac cgcctcttcc gccgcgccta cagctattcg
     4981 cgcggactcg cctcaagcgg acagcttgat gtcgggctgg tgttcgtctg ctatcaggca
     5041 aaccttgccg acggattcat cttcgtgcaa aacctcctca acggcgaacc gctggaagaa
     5101 tacatcagcc ccttcggcgg cggctatttc ttcgtcttgc ccggcgtgga aaaaggcggc
     5161 tttttggggc aagggctgct gggcgtataa atccgccata taaaaaacgc cgtccgaacc
     5221 ttgccaaacg ggttcggacg gcgtttcttg tttttgggcg gtcaggcttt tttgacgaat
     5281 tcggatttta aattcatcgc gctgccgtcg attttgcagc cgatgttgtg atcgccttct
     5341 tgcaggcgta tgcctttgac ttttgtgcct tgtttgatca ccatcgagct gccttttacc
     5401 ttgaggtctt tgatgaggat gacggtatcg ccgttttgca gcactgcgcc gttggcatcg
     5461 agcacttgag ccgcaaggtc ggcggcggat tcggtttcat tccattcgtg ggcgcattcg
     5521 gggcagatgt attgtccgcc gtcttcataa gtgtattcgg aggcgcattg tgggcatggg
     5581 ggtaatgaca tggtttgcag tccttatctg atatcggagg agtttgatgc cgtctgaaac
     5641 ctgaaaccgg cttcagacgg catagcttta ttgcttgtct ttttcaggcc gcatccagcc
     5701 ttcgatgacg gtttggcggg cgcgggcgag ggcgagtttg ttgtcttcga tattgcgggt
     5761 aatcgtgctg cctgcgcctg tggttacttt gttgccgagg gtaacggggg cgactaggac
     5821 gcagtttgaa ccgatgcgga cttcgtcgcc gataacggtt ttgtgtttgt gcacaccgtc
     5881 gtagttggca ataatcgtac ctgcgccgaa gttggttttg ctgccgactt cggcatcacc
     5941 aatgtaggtg aggtggttgg ctttggtgcc tttgccgatg gcggcgtttt tgatttcgac
     6001 gaagttgccg acgtgtacgt cggcggctag tttggcttgc ggacgcaggc gggcataggg
     6061 gccgattcgg ttgttttcgc cgacttcgca gctttcgagg tgggagaagg gggcgatttt
     6121 gctgtttgcg ccgattttgg cgtttttgat gacgcagttt gcgccgattt cgacgttgtc
     6181 gccgagttcg acttcgcctt caaagataca gttcacatca attacgacat cctgtccgtg
     6241 tttcagacgg cctcgtaaat cgaaacgtgc cggatcgcgc agggttacgc ctgctttgag
     6301 caattcttgc gcctgttcgg tttggaagat gcgttcgagt tcggcgagct ggcgtttgtt
     6361 gttcacgccg gcggcgaggt gggaggcgcg gacttggacg ggatgaactt taataccgtc
     6421 ggcaacggct ttggcaatga ggtcggttaa ataatattcg ccttgagcat tgttgctgga
     6481 gaggctgttc agccagtttt ccagtttggc gttgggcaga accaaaatgc cggtattgat
     6541 ttctttcacg gctttttggg cggcgtcggc gtctttttct tcgacaatgg cggttacgct
     6601 gccgctgccg tcgcggatga tgcgccccaa acctgacggg tcggcgggca cgtcggtcaa
     6661 cagcccgact tcgttgcctg cggcttcgag cagggtttcg agggtttcaa cgtcaattaa
     6721 aggaacgtcg ccgtacaaca ccagcgtgcg gccttcggcg gcaaggtggg gcagggcggt
     6781 tttgacggcg tggccggtac cgagctgttc ggtttgttca acccaaacga catcgcgttt
     6841 gacagcgttt aaaacttggt ctttgccgtg cccgatgacg acgcagatgt tttgcggatt
     6901 cagcgcggcg gcggtgtcga taacgcgccc gagcatgggc ttgccgccga tgcggtgcag
     6961 cactttgggc attttggaat acatgcgcgt gcctttgccg gcggcgagga taacgatgtt
     7021 taaagtgttt tgcggcatga cggtttcctg tgcaatgccg tctgaaaggg cttcagacgg
     7081 catagggtgg gtttatcggt tttgaaattt gggcttttgc cagtgttggc gatgctcttc
     7141 gtcggcgttg ttgccggttt ggttgggtaa cacggcatgg cgttcgggac ggtattggtt
     7201 gtagttcata tttttcgagt agctgccgtc ttggtaataa atgggcgtgc cggcgggata
     7261 tttttgacgg acggcggtct tgccgttgcc gtcttgataa gtttcccacg cgcagcccga
     7321 caaaagggcg gcggcagtca tcaggataag gaaggtttta cgcataacat tctttcgtat
     7381 tttcgggggg ttgtgcgggt attgtaatga ttttggcggt gttctgacaa agtttctgca
     7441 taccgagcca gttgcgccat atcgcttacg gaagcatcga taaagggcag gtcgtgcact
     7501 tttgcaccga accggacggt tttcatgccc aatgcttttg cctgatgcag gttgtccgcg
     7561 ctgtcgtcca ccataatgca gcattcgggc ggtacgtcca acaggcggca gacattgaga
     7621 tacgcttgcg gattgggttt gtacagcagc ccgaaatcat ccgtgccgaa aagcgcgtcg
     7681 aaacggtttt ccaaaccgag tgcgccgaca acggcacgga cgtaaaacga cgggccgttg
     7741 gaaaaaaccg ccttgcgccc ttttaggcgg ctcagggtgt tttgtgtttc cggcataccg
     7801 tgcagacggg tcaaaacctc gtcaatcgga tggctttcgc gcaaaaattc ggcgatgtcg
     7861 atttcgggat ggtggatttg cagtccggcg agcgttgcgc cgtagcggtg ccaatagtct
     7921 tgacgcaggt cggacgcggc ggattcggag agtttgaggc ggcgtgtcat atagcgtgtc
     7981 atagcgcggt tgatgagtgt gaagaggccg gcatcggcat tgtgcagcgt gttgtcgagg
     8041 tcgaacagcc acacggtcgg gttttcttgc attttgaacc gtgaaaattt gttagaatgt
     8101 tattttacag cgaatagagg aggactcgga atgaaacgga aaatttggct gctgccgctg
     8161 ctggcggttt cggcatacct gcaggcgcag acggaagtca ggctggcggt gcataagtcg
     8221 ttcagcctgc ccaaagggct gattgcgcgc ttcgagcggg caaacgatgc gaaggtgtcg
     8281 attattcagg cgggcggcgc gaacgaaatg ctcaacaaac tgattttgag ccgcgccaac
     8341 ccgattgccg acgcggtgta cggtttggac aacgccaata tcggcaaggc gcgggaaatg
     8401 ggcattttgg cggcggcgca acccgaatcc gcccccgtcg cggtcgggct gccttcggct
     8461 ttggcggtcg attacggcta tgtgtccatc aattacgaca aaaaatggtt tgaaggcaaa
     8521 aagctgcccc tgccgcaaac cctgcaggat ttgacccgcc ccgaatataa aaacctattg
     8581 gtcgtgccgt cccccgccac gtcgtccccg gggctgggct tcctgatggc gaacatcagc
     8641 ggtctgggcg aagaaggcgc gttcaaatgg tgggcacaga tgcggcagaa cggcgtgaag
     8701 gtcgccaaag gctggagcga ggcgtattac accgactttt cgcacaacgg cggcgcgtat
     8761 ccgctggtgg tcggttatgc cgccagcccg gcggcggaag tgtatttttc caaaggcaaa
     8821 tacagcgagc cgccgacggg caacctgttt ttaaaaggcg gcgtattccg ccaggtcgaa
     8881 ggcgcggcgg tcttgaaggg cgcgaaacag ccggaattgg cggcaaaact ggtgcaatgg
     8941 ctgcaaagtc gggaagtgca gcaggcggtt ccgtccgaaa tgtgggttta ccccgccgtc
     9001 aaaaacacgc gcctgcccga cgtgttccgc ttcgcccaag ccccgacgca caccaccgcc
     9061 cccgcgcagc gcgatattga tgcgaaccag cgcggatggg tttcccgttg gattagaacg
     9121 gttttgaaat aaaacaaaca taccgccccg cagggcttca tacggcattt ttacacctgt
     9181 gccgattacg ccgcacgggg cggatgttcg atcaagagga aaacaatgga cttcaaacaa
     9241 tttgattttt tacacctgat cagtgtttcc ggttgggagc atctggctga aaaggcgtgg
     9301 gcgttcgggc tgaaccttgc cgccgcgctg cttatttttt tggtcggaaa atgggcggcg
     9361 aaacgcattg tcgctgtgat gagggcggcg atgacgcgcg cgcaggtcga tgccacgctg
     9421 attagttttt tgtgtaatgt tgccaatatc ggcttattga ttttggtgat tattgccgca
     9481 ttgggcagat tgggcgtttc cacaacatcc gtaaccgcct taatcggcgg cgcgggtttg
     9541 gcggtggcgt tgtccttgaa agaccagctg tccaattttg ccgccggcgc gctgattatc
     9601 ctgttccgcc cgttcaaagt cggcgatttt atccgcgtcg gcggttttga aggatatgtc
     9661 cgagagatta aaatggtgca gacttctttg cggacgaccg acaacgaaga agtcgtgctg
     9721 cccaacagcg tggtgatggg caacagcatc gtcaaccgtt ccacactgcc gctgtgccgc
     9781 gcccaagtga tagtcggcgt cgattacaac tgcgatttga aagtggcgaa agaggcggtg
     9841 ttgaaagccg ccgtcgaaca ccccttgagc gttcaaaacg aagagcggca ggccgccgcc
     9901 tacatcaccg ccttgggcga caatgccatc gaaatcacat tatgggcttg ggcaaacgaa
     9961 gcagaccgct ggacgctgca atgcgacttg aacgaacaag tggtcgaaaa cctccgcaaa
    10021 gtcaatatca acatcccgtt cccgcaacgc gacatacaca tcatcaattc ttaaacgccg
    10081 tctgaaagag gagtgggaaa tggacgcgct gcacaccatc gcccgaaacc tgacgaaaaa
    10141 acgtcaaacc gtaagctgtg ccgaatcctg cacgggtggg ctgcttgccg ccgtattcac
    10201 aagcgttgca ggcagttcgc aatggttcga tcaaagtttt gtaacataca gcaacaaagc
    10261 caaagaagac cgcttgggcg tgttgcccga aaccctgctc gaacacggcg cggtcagccg
    10321 ccaaaccgtc tatgagatgg cgcgcggcgc gaaagccgtg gcgcaggcgg attacgccgt
    10381 cggtatttcc ggcatcgccg gtccgggcgg cggcagcgaa agcaaacccg tcggcacggt
    10441 ttggttcggg tttgcctttc cgggcggaag ttgcgaagca atgcgccgtt ttgacggcaa
    10501 ccgcgaatcc gtccgcgcgc aggcggtcgc cttcgcgttg gaacggttgg cggggctgat
    10561 tgaaaacggc ggcgatgccg tctaaacaaa atctccgtct ggacaaaatc cccgccggat
    10621 aaaaaatacc gtctgaaacg ttgcaggttt cagacggtat tttgtcgggt taggcggcgg
    10681 tgcggcttat ttcactttac tcttcaacgc gccgtagcct gccgcgtcca tttgttccag
    10741 cgggatgaat ttcaagctcg cgccgttgat gcagtagcgc agtccgcctt tgtcgcgcgg
    10801 gccgtcgggg aagacgtgtc ccaaatgcga atcggcggcg tggctgcgca cttcggtgcg
    10861 gcgcatgttg tagctgaaat catcgtgttc ggtaacggat tttgcatcaa tcgggcgcgt
    10921 gaagctcggc cagccgcagc cggaatcata tttgtcggcg gagctgaaca aaggttcgcc
    10981 gctgacaacg tccacataaa tgccgggttt gaacaaatgg tcgtattcgt ggctgaaggc
    11041 atattcggtc gcgctgtttt gggtaacttg gtattgctct tcggtcaggg tgcgtttgag
    11101 ttcggcgtca ctcggttttt tatacgttgc cgcgtcgaag cctttgcctt gcggtgcggt
    11161 cttggttttg cccggcagcg gttcgtcggc tttgcggata tcgatgtggc agtagccgtt
    11221 ggggttttta atcaagtagt cctgatggta ttcctcggca tcgtagaagt ttttcagcgg
    11281 ctcgttttca acaacgaggg gcagttggta tttttgctgc tcgcgtttga gggcggcggc
    11341 gatgacggct ttttcggcgg ggtcggtgta gtacacgccg ctgcggtatt gcgtaccggt
    11401 gtcgttgccc tgtttgttga ggctggtcgg atcaacgacg cggaagaaat attgcaggat
    11461 gtcgtctagg ctgagtttgt cggcatcgta ggtcacttta acggtttcgg cgtggcccgt
    11521 atggcggtag gacacgtctt catagctcgg atttttcgtg ttgccgttgg cgtagccgga
    11581 taccgcgtca accacgccgt cgatgcgttg gaaataggct tccaagcccc agaagcagcc
    11641 gccggcgagg taaatggtgc gcgtgttcat gatttttgaa tcctttttct gagtgtcggg
    11701 tttgtagaac gaatgtttca aactgcccaa atcggcattc gggtcgcgga ttaacgccaa
    11761 cgcctgcgct tcgttgatgc tgcctttgac gatgcgctgc acgtcgccgt ctttaccgat
    11821 taacgcccac gaagggtaaa cgctgatatt caggctttgg gcgatcgtgc cgccgttgtc
    11881 ggttacgacg ggcagcttgg gataattcaa accggcatac catttttgga agtcgccgtc
    11941 ttttttctcg tgcaaaaagc ccggagacgc gacggtaatc aggttggcgg agctgaattt
    12001 tgcatcttgc gcccattttt cggtctgtcc caattcggac agacacaaag gacaccagct
    12061 tgcccaaaat ttaatcagcg tcggtttgtc ttttttcaag taaacactgg cggggcggtt
    12121 gtctgcggtt ttcaaagtgg ataaagtgtg cggcacggtc gcggctccgg catcgacgat
    12181 tttgggcgaa caagcaccca gcgcaagcag gcagccgaac ttggcgcaaa gggaaaagaa
    12241 agtacggtgt ttcattttaa tgtttcctgt gtggacggtt tgcatgatta gacgtttgag
    12301 atgccgaaac cttacagccc ggattttcag acaaccttgc cgcgcaaaat acgctacaat
    12361 acaccctatt tcaagtttct aaaattaaaa ggaaaattca atgttcagct tcttccgtcg
    12421 caagaaaaaa caggaaacgc cggctcccga ggaggctcaa gttcaggaaa ctgcagcaaa
    12481 agcagaatcc gaagttactc aaatagttga aaatattaaa gaagaagtcg aatctttaac
    12541 ggaaagcgtc aaagggcagg tcgaatcggc tgttgaaacc gtcagcggtg cggttgaaca
    12601 ggtaaaggaa accgttgccg agatgctgtc tgaagcagag gaagcggcgg aaaaagcagc
    12661 ggaacaagtc gaagcggcaa aagaagccgt tgccgaaacc gtcggcgagg ctgtcgggca
    12721 agttcaagaa gccgttgcga caactgaaga acacaagctc ggttgggcgg cgcgtttgaa
    12781 acaaggtttg accaaatcgc gcgacaaaat ggcgaaatcg ctggcgggcg tgttcggcgg
    12841 cggacaaatc gacgaagatt tgtacgaaga gctggaaacc gtgctgatta ccagcgatat
    12901 gggcatggaa gccaccgaat acctgatgaa agacgtgcgc gaccgcgtca gcctcaaagg
    12961 gctgaaagac ggcaacgaat tgcgcggcgc gctgaaagaa gccttgtacg acctgattaa
    13021 gcctctagag aaaccgctgg ttttgcccga aaccaaagag ccgtttgtca tcatgcttgc
    13081 tggcatcaac ggcgcgggca aaaccacatc catcggcaaa ctcgccaaat atttccaagc
    13141 gcagggcaaa tctgtgctgc ttgccgccgg cgatactttc cgcgccgccg cgcgtgagca
    13201 gcttcaagct tggggcgaac gcaacaacgt aaccgtgatt tcgcaaacca cgggcgattc
    13261 cgccgccgtg tgcttcgatg ccgtccaagc cgccaaagcg cgcggcatcg acattgtgct
    13321 ggccgacacc gccggccgcc tgcccacgca gcttcacttg atggaagaaa tcaaaaaagt
    13381 gaaacgcgtg ctgcaaaaag ccatgcccga cgcgccacac gaaatcatcg tcgtgcttga
    13441 tgccaatatc ggtcaaaacg ccgtcaacca agtcaaagcc tttgacgacg cattggggct
    13501 gaccggttta attgttacca agctagacgg cacggcaaaa ggcggcatcc tcgccgcgct
    13561 tgcctccgac cgccccgtcc ccgtccgcta tatcggcgtg ggcgaaggca tagacgattt
    13621 gcgcccgttt gacgcgcgcg cgtttgtgga cgcactgctg gattgagccg aaatgccgtc
    13681 cgaaaacagc agaccgatgc cgtcattccc gcgcaggcgg gaatccagac cttgggataa
    13741 cggcaatatt caaaggttat aaaagacccg tcattcccgc gcaggcggga atctagacct
    13801 tagaacaaca gtaatattca aaggttagct gaagcttt
//
LOCUS       DAXHTR010000046        13799 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.046, whole genome shotgun sequence.
ACCESSION   DAXHTR010000046 DAXHTR010000000
VERSION     DAXHTR010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13799
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.046"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(14..1015)
                     /gene="hemB"
                     /locus_tag="ACFNML_000911"
     CDS             complement(14..1015)
                     /gene="hemB"
                     /locus_tag="ACFNML_000911"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFC6392320.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAEEAYNPE
                     GLVPAAVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALNEVALDIREGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAIANGWLDGGKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(1198..1482)
                     /gene="yhbY"
                     /locus_tag="ACFNML_000912"
     CDS             complement(1198..1482)
                     /gene="yhbY"
                     /locus_tag="ACFNML_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFC6392321.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            1592..2212
                     /locus_tag="ACFNML_000913"
     CDS             1592..2212
                     /locus_tag="ACFNML_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HFC6392322.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINDKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGSSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            2276..4243
                     /gene="ftsH"
                     /locus_tag="ACFNML_000914"
     CDS             2276..4243
                     /gene="ftsH"
                     /locus_tag="ACFNML_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFC6392323.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDKSVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASTESEQQPENKA"
     gene            4464..4895
                     /locus_tag="ACFNML_000915"
     CDS             4464..4895
                     /locus_tag="ACFNML_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HFC6392324.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPHFLPWYSKTEVIGRSG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            4888..5166
                     /locus_tag="ACFNML_000916"
     CDS             4888..5166
                     /locus_tag="ACFNML_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFC6392325.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            5219..5797
                     /gene="pth"
                     /locus_tag="ACFNML_000917"
     CDS             5219..5797
                     /gene="pth"
                     /locus_tag="ACFNML_000917"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFC6392326.1"
                     /translation="MSNKIKMLVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARAALPDGDVWLLKPATFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIDHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILAGKWEEATRFLHSK"
     gene            5858..6064
                     /locus_tag="ACFNML_000918"
     CDS             5858..6064
                     /locus_tag="ACFNML_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HFC6392327.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            6061..6423
                     /locus_tag="ACFNML_000919"
     CDS             6061..6423
                     /locus_tag="ACFNML_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HFC6392328.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            6654..8069
                     /locus_tag="ACFNML_000920"
     CDS             6654..8069
                     /locus_tag="ACFNML_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFC6392329.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKTAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            8188..8697
                     /locus_tag="ACFNML_000921"
     CDS             8188..8697
                     /locus_tag="ACFNML_000921"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6392330.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKNGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            8864..10246
                     /locus_tag="ACFNML_000922"
     CDS             8864..10246
                     /locus_tag="ACFNML_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HFC6392331.1"
                     /translation="MASIARDIFKAYDIRGIVGKTLTDEAAYLIGKAIAAKAAEKGIT
                     RIALGRDGRLSGPELMEHIRRGFTDSGINVLNVGMVATPMLYFAAVNECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYLKHI
                     TGHIRLKRPMNIAIDAGNGVGGAFAGKLYKGLGNEVTELFCDVDGTFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWIKEHGGEPVMEKTGHSFIKSAMKKTGAPVAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSASDNPSEVLNNLPQSISTPELNIALPEGSNGHQVIDELAAK
                     AEFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(10266..11021)
                     /locus_tag="ACFNML_000923"
     CDS             complement(10266..11021)
                     /locus_tag="ACFNML_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFC6392332.1"
                     /translation="MIKIRNIHKTFGENTILRGINLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPKHERTRRFLSQIQSTKI"
     gene            complement(11031..11747)
                     /locus_tag="ACFNML_000924"
     CDS             complement(11031..11747)
                     /locus_tag="ACFNML_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFC6392333.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAVALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(11737..12564)
                     /locus_tag="ACFNML_000925"
     CDS             complement(11737..12564)
                     /locus_tag="ACFNML_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6392334.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSESGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            12849..13757
                     /locus_tag="ACFNML_000926"
     CDS             12849..13757
                     /locus_tag="ACFNML_000926"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HFC6392335.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMSIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDGTENPQGDENVREVAI
                     IPEGKPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRGKETDDEFGRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLQHLSAAVSGGYYYAPSVERLQNL"
BASE COUNT         3389 a         4057 c         3402 g         2951 t
ORIGIN      
        1 acccggccga aaatcaacgc ttcaacatct ttgccgcctc aatagcgtaa taggtcaaaa
       61 tcccgtccgc acccgcacgt ttgaatgcca gcaggctttc caaaaccact ttgccgccgt
      121 ccagccagcc gttggcaatc gctgcctgca acatcgcgta ttctcccgaa acctgatagg
      181 cataagtcgg tacgccgaac tcgtccttca cgcggcggac aacgtccaaa tacggtaaac
      241 cgggcttcac catcaccata tccgcacctt cccgaatatc gagcgccact tcattcagcg
      301 cctcatcggt atttgccgga tccatctggt aggttttctt gtccgccttg cccaaattgc
      361 ccgaactgcc caccgcatcg cggaacggcc cgtaaaacgc cgaagcatat ttggcggaat
      421 acgccataat ccgcgtgtgg atatgcccgg catcctccaa cgcctcgcga atcgcaccga
      481 tacgcccgtc catcatatcg gaaggggcaa ccacctgtgc gcccgcttca gcgtggcaca
      541 aagcctgctt gaccaaaacc tctacggttt catcgttcat cacataaccg ttttcgtccg
      601 tcagcccgtc ctgaccgtga accgtataag gatcgagcgc gacatccgtc ataatgccca
      661 gttcgggaaa cctctcgcgc agggcgcgga cggctgccgg cacgagtcct tcgggattgt
      721 acgcttcctc tgcacgctcg gttttgtttg ccgtaaccac gggaaagagt gccaacatcg
      781 gaataccgag ctttaccgcc tcttccgccg taaacagcag cctgtccaaa ctttgacgct
      841 tcacacccgg catagaaggc acatcctcct cgcgcgccga cccctccaat acgaacaccg
      901 gataaatcaa atcatcggcg gtcagcgtgt gttcgcgcat caggcggcgt gaaaaatcgt
      961 ccctgcgcat acggcgcata cgcgaagccg gaacattgcg gtaaggaaac tgcataagcc
     1021 ctccaatcat cctgtcaaca attcaaacca tacggaagcc gccgcaactt gcagacagca
     1081 tcgggcggca atcaaaatcc gttttcaccg gaaagggttt cggcccgccg aaatgccgcg
     1141 ccgaaggacg gcgtcccgcc cattaaagaa caatagcaaa acaacagctt caggctgtca
     1201 gccttcgata ttcttacgcc acaataccaa aagtttcccg atatgctgaa caggttgcgc
     1261 gtcaaccgcc tcacataagg cagtgcagat ttcgatacgt tcggcacggt cgtcgccgaa
     1321 tacgcgcact ttaatcagct catgcgccgt caatgccgca tcggtttcct tgatgaccgc
     1381 gtccgtcaga ccctgctgac cgaccatcac aacaggatgg agatggtgcg cgcgcgcttt
     1441 cagttccaaa atttctttgg tgttcaattt ggtatccgtc attttcctac tgcttgaata
     1501 aaaagaataa tgcggcattg tacgcgattt ggcacgacat cggcaaaatt aacgtacaat
     1561 acgcggtttc cattccgacc cgcgaaccac tatggctgta cgttccaaat cctcaaaagc
     1621 gtggctgcac gaacacgtca acgaccacta cgtccatatg gcgcaaaaag acggctaccg
     1681 cgcccgtgcc gcatacaaac ttttggaaat caacgacaaa gacaaattaa tcaaacccgg
     1741 cacggtactt gccgacttgg gcagcgcgcc gggaagctgg tcgcaggttg ccgccaagct
     1801 gacgggttct tccggagcag ttttcgcctt ggacatcctg cctatggaag ccataggggg
     1861 cgtctccttc attcagggcg acttccgcga gaacgacgta ctggcacaat tcgaaacctt
     1921 gttggacaac cgcccgctcg accttgtaat ttgcgatatg gcgcccaata tgtcgggaaa
     1981 cgccgtaagc gatcaggcac gcagctttta tctgtgcgaa ctggctttag acttcgcctc
     2041 gcaacacctg aaaaccggcg gcagcttttt ggtcaaagtc tttcagggtg caggctatca
     2101 ggaatacatg gcagccatgc gcgaaatttt cggcacggtg cagacgcgca aacccgaagc
     2161 ctcgcgcaat cgctccagtg agatttattt attgggcaaa aataaacgct gacaatacag
     2221 acggcgtgct ttacaatcat ttccgtttta cacctcaatt atggagcctt gctaagtggg
     2281 gaacaccttt aaatcaatcc ttgtctgggt cgccttgggt atcggcctga tggctgcgtt
     2341 caacgcttta gacggtaaaa aagaagacaa cgggcaaatc gaatattctc agttcatcca
     2401 acaggtcaac aacggcgaag tatccggcgt caacatcgaa ggatccgtcg tcagcggcta
     2461 cctgattaag ggcgagcgca ccgacaaaag caccttcttc accaacgcgc ctttggacga
     2521 caacctgatt aaaacactgc tcgacaaaaa cgtccgtgta aaagtaacgc cggaagaaaa
     2581 accgagcgcg ctggctgccc tgttttacag cctgctgccc gtcctgctgc tgattggcgc
     2641 gtggttctac tttatgcgta tgcagacggg cggcggcggc aaaggcggcg cattctcatt
     2701 cggcaaaagc cgcgcccgcc tactggacaa agatgccaac aaagtgacct ttgccgatgt
     2761 cgccggctgc gacgaagcca aagaagaagt gcaggaaatc gtcgattacc tcaaagcgcc
     2821 gaaccgctat caaagcctcg gcggtcgcgt gccgcgcggc atcctgctgg cgggcagccc
     2881 gggtacgggt aagacgcttt tggcaaaagc cattgcaggc gaagccggcg tgccgttctt
     2941 cagcatttcc ggttccgact ttgtcgaaat gttcgtcggt gtcggtgcga gccgcgtccg
     3001 cgatatgttc gagcaggcga agaaaaacgc tccctgcatc atctttattg atgaaatcga
     3061 cgcagtcggc cgccaacgcg gcgcaggttt gggcggcggc aatgatgagc gcgagcaaac
     3121 attaaaccaa ttgttggttg aaatggacgg ttttgagagc aatcagactg taattgtgat
     3181 tgcggcaacc aaccgccccg acgtactcga tcctgcgctg caacgccccg gccgtttcga
     3241 ccgccaagtg gttgtccccc tgccggacat ccgggggcgc gaacagattt tgaacgtcca
     3301 ttctaaaaaa gtgcctttgg acaaatctgt ggatttattg tccctcgcgc gcggcacgcc
     3361 gggtttttcc ggcgcggatt tggcgaactt ggtcaacgaa gccgccctgt ttgccggccg
     3421 ccgcaataaa gtcaaagtcg atcagagcga ttttgaagac gccaaagaca aaatctatat
     3481 gggtccggaa cgccgcagta tggtgatgca cgaagacgaa aaacgtgcga cggcgtatca
     3541 cgaatccgga cacgcgattg ttgccgaaag cctgcccttt accgaccccg tccacaaagt
     3601 aaccattatg ccgcgcggac gtgcgctggg tctgacttgg cagcttccgg agcgcgaccg
     3661 catcagtatg tataaagatc agatgttgag ccagctctcc atcctgttcg gcggacggat
     3721 tgccgaagac atcttcgtcg gacgcatctc caccggcgca tcaaacgact ttgaacgcgc
     3781 aacccaaatg gcgcgcgaaa tggtaacgcg ctacggcatg agcgacaaaa tgggcgtgat
     3841 ggtttatgcg gaaaacgaag gcgaagtctt cttaggacgc agcgtaaccc gttcgcaaaa
     3901 catttccgag aaaacccagc aggacatcga tgcggaaatc cgccggattt tggacgagca
     3961 gtatcaggtt gcctacaaaa tcctcgatga aaaccgcgac aagatggaaa ccatgtgcaa
     4021 agccctgatg gaatgggaaa ccatcgaccg cgatcaggta ctggaaatca tggcgggcaa
     4081 acaacccagt ccgcccaagg attacagcca caacctgcgc gagaatgcgg acgcggcgga
     4141 agataacgcg ccgcacgctc cgactcggga agaaaccgaa gcacctgccc cggcagacac
     4201 cgcttcgaca gagtccgagc agcagcctga aaacaaggct taacttcccg aacaaacggc
     4261 agcccgcaag ctgccgtttt cccatcctgt tttcagacgg cattttccgc tcaatgccgt
     4321 ctgaaacgcc aaagccgtat cggtttttgc ggatctgcga tatggcggtt taaattgaaa
     4381 tcagacgagg caaggctgta ccggtttaaa attaaaccgc tataataccg cctttcgaat
     4441 acacgcccgc attggtgatg cctgtgaaaa aagttgaaaa aaacatcttg gtgctgcacg
     4501 gcgcggacaa aatgttcgag ctggtcgata aggttgaaga ctatccgcac tttctgccgt
     4561 ggtacagcaa gaccgaggtc atcgggcgta gcggcaacga actgaaggcg cggctgttta
     4621 tggactatat gcacgttcgc caatcattcg ccacgcacaa ccgcaacatt ccgggcaggg
     4681 aaatccgtat ggaactgctc gagggtccgt tcaaaacctt acgtggaacg tggaaattta
     4741 tcgatttggg cgacgatatg tgcaaaatcg aattcaattt ggaatacgat ttttccaatg
     4801 ccgttttgtc cgccttaatt tcccccgtct tcaaccacct ttccgccacg ctggtcgaag
     4861 cgttcgtcaa agaggcagac cgccgttatg cttgaaattg agattgtgta cgggctgccc
     4921 gaccgacagg ttttgaaaac catgcagctt gccgagggaa caaccgtccg cgccgccgca
     4981 ctgcaaagcg gtttggacgg catatttgaa gatttaaacc tgcattccgc gcctttgggc
     5041 attttcggca aagccgtcaa agacgacacg cccctgcgcg acggcgaccg catcgaagtg
     5101 taccgcccgc tgttgatcga ccccaaagaa gcgcgccgca aacgcgttca aaatcaagaa
     5161 gaataaccat gccgtctgaa gccttcagac agcattcgac agaaacacgg aaaatatcat
     5221 gtcaaacaaa atcaaaatgc ttgtcggatt gggcaacccc ggcaaagaat acgaacaaac
     5281 ccgacacaat gcgggttttt ggttcctcga cgaactggcg tggaaatgga aggcttcatt
     5341 taaagaagaa aaaaaattct tcggcgaagt cgcccgtgcc gccctgcccg acggcgacgt
     5401 ttggctgctc aaacctgcca cgttcatgaa ccgttccgga caggcagttg ccgcgcttgc
     5461 acagttctac aaaatcaaac ccgaagaaat cctcgtcgtc cacgacgaac tcgacattcc
     5521 ctgcggacgg attaaattca aactcggcgg cggcaacggc ggacacaacg gcttgaaaga
     5581 cattcaggca aaactcggca cggcagacta ttaccgcctg cgcctcggta tcgaccaccc
     5641 gggcgaccgc aacctcgtcg tcggctatgt cctgaacaaa cccagtacgg aacaccgccg
     5701 acagattgac gatgccgtcg ccaaatccct gcaagccata cccgacatcc ttgccggcaa
     5761 atgggaagaa gcaacccgct tcctgcacag caaatgaccc gatgccgtct gaagcccttt
     5821 cagacggcat gttcccgatt tccatatccg aacagtcatg accgaactca agcagcttat
     5881 ccaaaccgaa tccatccccg tcatcgaaga aaccctcgat ttcctgctgt acgaatgcag
     5941 catcgacgac gcaccatccg ccgaagaagt ggcacaatgg cgcgacatac ttgccgcacg
     6001 cggcggcaaa ttcctgcgcc tgtccaaaat ctgccaaacg tggctggacg aggaggcggc
     6061 atgaagctgc cgcgcaaccg cttcagcctg ctttccgcat tgtggtttgc cggcggcatc
     6121 tattcgctgc tcttcaaagc tgccgacacc gcgccgccgc cgtttccgca tttcgacaaa
     6181 gcagcacacc ttgccctgtt tttcgcacaa atctggcttt tgaccaaagc attcaaaacc
     6241 ggaaaacttc ccatccccta ccgcagcctg atggtctttg ccctctgttt cgccctcttc
     6301 agcgaatgcg cgcaggcatg gtttaccgca acgagaaccg gcagtttggg cgatgttctt
     6361 gccgatatgg caggtacggt tctcgcactc tttgccgccc gcgccgccga ccgcccggac
     6421 tgaatcggtt tatttccccc aaacaggatg cacctcttcc cgatacatcc tatttttccc
     6481 cttacatatc aatcccatct cataaacaaa accgacaaat cgtttacaat atatttacac
     6541 tacatcagat tacaaatata ctcgaaccag ttgtagaatc ggcggttcca caaaccgttt
     6601 cggattgcac gacacaaaag aaaaaccgat tttattgcct taaaggagta ttcatgaacc
     6661 tgcatgcaaa ggacaaaacc cagcatcccg aaaacgtcga gctgctcagt gcgcagaagc
     6721 cgattaccga ctttaagggc ctgctgacca ccattatttc cgccgtcgtc tgtttcggca
     6781 tttaccacat cctgccttac agccccgatg ccaataaagg tatcgcgctg ctgattttcg
     6841 ttgccgcact ttggtttacc gaagccgtcc acattaccgt aaccgcactg atggtgccga
     6901 ttctcgccgt cgtactcggt ttccccgaca tggacatcaa aaaggcgatg gctgattttt
     6961 ccaacccgat tatctacatt tttttcggag gcttcgcgct tgccaccgcc ctgcatatgc
     7021 agcggctgga ccgtaaaatc gccgtcagcc tgttgcgcct gtcgcgcggc aatatgaaag
     7081 tggcggtttt gatgttgttc ctcgttaccg cctttctgtc catgtggatc agcaacaccg
     7141 ccaccgccgc gatgatgctg cctctagcaa tgggtatgct gagccacctc gaccaggaaa
     7201 aagaacacaa aacctacgtc ttcctcctgc tcggcatcgc ctattgcgcc agcatcggcg
     7261 gcttgggcac gctcgtcggc tcgccgccca acctgattgc cgccaaagcc ctaaatctgg
     7321 acttcgtcgg ctggatgaag ctcggcctgc cgatgatgct gttgattctg cccttgatgc
     7381 tgctctccct gtacgtcatc ctcaaaccta atttgaacga acgcgtggaa atcaaagccg
     7441 aatccatccc ttggacgctg caccgcgtga tcgcgctgtt gattttcctt gccacagccg
     7501 ccgcgtggat attcagctcc aaaatcaaaa ccgccttcgg catttccaat cccgacaccg
     7561 ttatcgccct gagtgccgcc gtcgccgtcg tcgtcttcgg cgtggcgcaa tggaaggaag
     7621 tcgcccgcaa taccgactgg ggcgtgttga tgctcttcgg cggcggcatc agcctgagca
     7681 cgctgttgaa aacatccggc gcgtccgaag ccttgggaca gcaggttgcc gccacctttt
     7741 ccggcgcgcc cgcatttttg gtgatactca tcgtcgccgc cttcattatt tttctgaccg
     7801 agttcaccag caacaccgcc tccgccgcat tgcttgtacc gattttctcc ggcatcgcta
     7861 tgcagatggg gctgcccgaa caagtcttgg tattcgtcat cggcatcggc gcatcttgtg
     7921 ccttcatgct gccggttgcc acaccgccta acgcgattgt gttcggcacg ggcttaatca
     7981 agcaacgcga aatgatgaat gtcggcatac tgctgaacat cctctgcgta gtattggttg
     8041 ctctgtgggc ttatgctgta ctgatgtaaa ccatcgacct aaacaacaag accgtctgaa
     8101 agaatatttt tcagacgacc ttgaagtttg tcgttcagac acaatttgtc gaatcattca
     8161 aaaccggatt ctaacgaaag gaaacccatg attatcctgc acaccaacaa aggcgacatc
     8221 aaaatcgaac tcgatttcga caaagccccc gttaccgcca aaaacttcga gcaatacgtc
     8281 aaaaacggct tctacgacgg cgtaatcttc caccgcgtca tcaagggctt catgattcaa
     8341 ggcggcggca tggatgaaaa catgaacgaa aaagaaaccc gcgatccgat tcaaaacgaa
     8401 gcgtccaacg gcctgcccaa cgataaatac accatcgcca tggcgcgtac ttccgatccc
     8461 cattccgcca gcgcgcaatt cttcatcaac actgccgaca acgctttcct gaacttccgt
     8521 tctaaagagc tgtacggcaa aaccgtcgtc caagactggg gctatgccgt attcggtaaa
     8581 gtcgttgacg gttttgacgt tgtcgatgcc atcgaaggcg tttctaccaa acgtcatggt
     8641 taccacgacg acgtaccgag cgaacctgtc gtcatcatta aagccgaagc ggtataaacc
     8701 gacaatccga aagcagcctg cacaaaggct gcttttattt cagacggcat gttatttgat
     8761 gtaaccgtgc tatgccgtct gaaaaccgga cgcaagcctt cagacggcat accgctatgt
     8821 taaaatatgc ccgtttttcc taaacggaca caaaggaaac cttatggcaa gcatcgcccg
     8881 cgacatcttc aaagcctacg acatccgggg tattgtcggc aaaaccctga ccgacgaagc
     8941 cgcctacctt atcggcaaag ccatcgccgc caaagccgcc gaaaaaggca ttacccgcat
     9001 cgcgctcgga cgcgacggac gcttgagcgg tcccgaactg atggaacaca tccggcgcgg
     9061 ctttaccgac agcggcatca atgtcctcaa tgtcggtatg gttgccactc ctatgctcta
     9121 cttcgccgcc gtcaacgaat gcggcggcag cggcgtgatg attaccggca gccacaatcc
     9181 gcccgattac aacggcttta aaatgatgct cggcggcgac acgcttgccg gcgaagccat
     9241 ccaagaactt ttgtccatca ttgaaaaaga cggttttgct gccgccggca aacaaggcag
     9301 cgtcaccgaa aaagacatct ccggcgaata cctcaaacac attaccggac acatcaggct
     9361 caaacgcccg atgaacatcg ccattgacgc gggcaacggc gtgggcggcg cgtttgccgg
     9421 caaactctac aaaggcttgg gcaacgaagt aaccgagctt ttctgcgacg tggacggcac
     9481 tttccccaac caccatcccg acccatccaa accgaaaaac ctgcaagatt tgattgccgc
     9541 gctgaaaaac ggtgatgccg aaatcggctt ggcgtttgac ggcgatgccg accgcttggg
     9601 tgtggtcacc aaagacggca acattattta tcccgaccgc caactgatgc ttttcgcgca
     9661 agacgtgttg agccgcaatc ctaaagccaa agtgattttc gacgtcaaat ccacccgtct
     9721 gcttgcgcct tggattaaag aacacggcgg cgaacctgtg atggaaaaaa ccggccacag
     9781 ctttatcaaa tccgccatga aaaaaaccgg cgcgccggtt gccggcgaaa tgagcggaca
     9841 catcttcttc aaagaacgct ggttcggctt cgacgacggt ctgtacgccg gcgcacgcct
     9901 cttggaaatc ctgtctgcct ccgataatcc gtccgaagtg ttaaacaacc tgccgcaaag
     9961 catttccacg cccgaactca acatcgccct gcccgaaggc agcaacggcc atcaggttat
    10021 cgacgaactc gccgccaaag ccgaatttga aggcgcaacc gaaatcatca ccatcgacgg
    10081 tctgcgcgtt gaatttcccg acggcttcgg tctgatgcgt gcttccaata ccacgccgat
    10141 tctggtgttg cgttttgagg cggatacgca agaagccatc gagcggattc aaaaccaatt
    10201 caaagccgtc atcgaaagca atccgaatct aatctggcct ctgtaaacac aggaaaaatg
    10261 cttaatcaaa tcttggtaga ttggatttgg cttaaaaatc tccgcgtccg ttcgtgtttg
    10321 gggtggtcga acaactcttt cgggctgccc tgctctacga taacgccgcc gtccatcacg
    10381 acaacggtag tcgcaacttc cagcgcgaac ttgatttcgt gggtaacgac gaccatcgtc
    10441 caaccttccc gcgccaattc cttcatggcg ttcaacacgt cttgcaccaa ctcggggtca
    10501 agcgcggaag tcggttcgtc aaacagcatc agctcgggct gaatcgccaa tgctcgggca
    10561 atgccgacgc gctgctgctg accgccggaa agctggtagg gatacaaatc cactttgtcg
    10621 cccaagccga ctttttccag cagtttcaga gcctcttcgc gcgcttgggc ggcaggcttg
    10681 ccctgtacgg caaccggtcc ttccatcacg ttttccaagg cggttttgtg cggaaagagg
    10741 ttgtattgtt gaaacaccat gcctgatttg cggcgcagtg ccaaaatatc gtgtttgctt
    10801 ggttttttag aaaaatcgat tttcagcggt cgctcgttgt cgaactcgat ttgtccgtct
    10861 tcgggcattt ccaacgcgtt taggcatcgc agaaacgtcg ttttgcctga gccggaaggc
    10921 ccgaggatga cgaccacctg ccctttgcac acatccaaat tgatgccgcg caaaatggta
    10981 ttttcgccga aggtcttatg gatattgcgg attttaatca tgacaactcc ttatttggcg
    11041 acatagcggt cgaagcgttt ttccaagcgc gcctgaatca ggaacagcac tttgcaaaaa
    11101 caccagtaaa ccaaagcggc ttcgatatag acgggcaaaa agtcataagt gcggtttgcc
    11161 gtttcctgcg cgacgcggaa taattccgtt accgtcacga ctgccgcgag cgaggtgttt
    11221 ttaaacaaac cgataaactc gttgctcaaa ggcggcacgg caacgcgaaa tgcctgcggc
    11281 gcgacgatgc ggcggaacgt ctgcatatag gtcatgccga tggagaaacc tgcttcccat
    11341 tggcctttcg gtacggacaa aattgccgcg cgtatggttt cggaagcata tgcgccgaca
    11401 ttgagcgaaa agccgatgat ggcggcagga atcgggtcga tatagatgcc gacggaaggc
    11461 agcccgtaaa acacaatcac aagctgaacc aacagcggcg taccgcgaat gacggaaata
    11521 taaaattcca ccaatttcag caggattttc cgcacgatgc cgccggcggg cataatccgc
    11581 accaaagcca cggctaccgc aatcatcata ccgataacga aagaagctgc cgccaaaggc
    11641 agagagaccg cgaagccggc tttgaccata ggcaaaaacg cgctgacaat catatcggcg
    11701 cgtgtttccg tcataaacgg cagcgaagca aggaaattat tgaacactga tgtcttttcc
    11761 gaagaattgt tcgcccagtt ttttcagcgt accgtcggct ttcagctcgt tgattgccgt
    11821 actgaatttc gccacggctt cgtcattgcc cttgttgaca atcaggccgg aaccgacttt
    11881 ttcatcggca ggtgcggacc aaacgatttt cacgcccgca ttcgggtttt tcttcagata
    11941 gtccaaaacc gccaattcgt cgttcagggt tgcatcggca cgtttttgtt caatcagggt
    12001 cagcgattgc gccaaaccgt ccactgccac caaatctgcg cctgcagctt tggctttttc
    12061 gccgtagttg ctggtcaggg attgtgcggt tttcacgcct ttgatgtcgt cgatagattt
    12121 gatgttgctg tcgttacggg caaccaatac ggcaccgctc cagctgtaag gatcggattt
    12181 gtcgaatgtc gcttggcgtt cggggctggt cagaccgact tggtttgcca ccacgtcgaa
    12241 acgccccgcc ttcaaacccg ccatcatcga atcccattgc gtttctttaa actcgacttt
    12301 cacgcccagt ttttccgcca cggcgcgggt tacttccaca tcgtaaccgg tcagtttgcc
    12361 gtccttgtcg tggtaggtaa acggtgcgta agtgccttcc gtgccgacgg taaccgtgcc
    12421 tttattgttg atgcgctcga ttaaagaacc ggaaactgcc gattgtgcag gcgcggaaga
    12481 tgctccgctg ccgctttccg aaccgccgca ggccgccaaa accaatgcgg caatgccgcc
    12541 gagtacgaat tttttcaaca tcattctctc ctgtaaaaag tgtgcaagtg cggcattcta
    12601 agtgaaattg cggcaaacac aaagaatact ttttttatat ggttattcca aatcggaatg
    12661 gaaaagatat gccgaagccg ccggaagcgg aaataaaggg ggcgggaaag gcgacgggcg
    12721 gcacgaaacc gaaaagcggc atcaggtatg caaacggcgg attcacgcac gccgttccgg
    12781 caaaaatccg cctgccgatt cggtgctata atccatccga aacagatgac accaaaaagg
    12841 aacatctcat gaacacgccc caatccgcca tcattcccga ccacgcccaa gccggcatct
    12901 ttatcgaagc cgacttcgcc gccaaccgcc tcaacgatat taaagccgcc tgccgcgctt
    12961 cgctcgacgc gttatccgcc ttgaaaaccc gctttccgga cgatattttg ggtctgacca
    13021 tcgccttcgg cagcaaagcc tgggcaacat tcggacatac ggacgaaggc agtgaaatca
    13081 aacccttctc cgaaatgggc aacgggcttg cgccgtccac gcagcacgat atgtccatcc
    13141 acatccaatc tttccgccaa aacgccgcct acgcgcttgc ccaatccgtt ttgggcgcgt
    13201 tcggcgacag catatgcgtc gcgtccgaag aacacggttt gcgcctgtat caggatcgcg
    13261 ggctggacgg tttcgtcgac ggcacggaaa acccgcaggg cgatgaaaat gtccgcgaag
    13321 tcgccatcat ccccgaagga aaacccgatg cgggcggcag ctatgtcctg ctgcaaaaat
    13381 acctgcacga tttgaaaaaa tgggatgccg tccccgtcgc cgaacaggag gcctcggtcg
    13441 gacgcggcaa ggaaaccgac gacgaattcg gccgcgatgt ccgcctgccc gattcgcacc
    13501 tcggccgcgt caacctgaaa gaaaacggag tcggcctgaa aatcgtccgc cgcagcctgc
    13561 ccttcggcaa aatcagcggc gaacacggtt tgatgtttac cgcatactgc cgcacgctgc
    13621 acaatatcga agcgcaatta ttgagtatgt tcggcgatac ggacggcaaa accgacctgc
    13681 tgctccaaca tctctccgcc gccgtttcgg gcggatacta ctacgcgccg tctgtcgaac
    13741 gcctgcaaaa cctctaagcc ggacgatgcc gtctgaaacc atttttcaga cggcatttt
//
LOCUS       DAXHTR010000047        13797 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.047, whole genome shotgun sequence.
ACCESSION   DAXHTR010000047 DAXHTR010000000
VERSION     DAXHTR010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.047"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..278)
                     /locus_tag="ACFNML_000927"
     CDS             complement(<1..278)
                     /locus_tag="ACFNML_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6392336.1"
                     /translation="MITVEHLEHYLGEIENGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPI"
     gene            complement(275..1045)
                     /locus_tag="ACFNML_000928"
     CDS             complement(275..1045)
                     /locus_tag="ACFNML_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6392337.1"
                     /translation="MVKTADGYKAIARIRVGDRVFAKDEASGKTGYKPVTTRYGNPYQ
                     ETVYIEISDGIGNNQTLISNSIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGDRAETEGVWVHNDCPYGSSSAKGKSGSERNTAKRGT
                     FRQETLKNAWDLAADGSQPNSKKCPTCGKDVYGNPHNKEKRNTKDGWDGSHNPSWSNR
                     QHSSDRKEQLNDYNKGVSLECRHCNRSGGNKDERFKKK"
     gene            complement(1260..1619)
                     /locus_tag="ACFNML_000929"
     CDS             complement(1260..1619)
                     /locus_tag="ACFNML_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392338.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDS
                     YKVHWKINNGNLIQTTN"
     gene            complement(1840..2295)
                     /locus_tag="ACFNML_000930"
     CDS             complement(1840..2295)
                     /locus_tag="ACFNML_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392339.1"
                     /translation="MVDAVVKTPEFLPFTSVGIFRFGADITQYKNILGTFMYEPPDEF
                     RTEYYESPDSNLLIAVKKNKIISIFCYQELYFMGVNIIGLNFEDFKQLFHHPKSYGVD
                     KYYLSNESYPTYVYEFDEIGVQAWEAKGKIVTIIAGGKDNYSTEPYYDE"
     gene            complement(2276..2905)
                     /locus_tag="ACFNML_000931"
     CDS             complement(2276..2905)
                     /locus_tag="ACFNML_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6392340.1"
                     /translation="MVKTADGYKAIARIRTGDRVFSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIKVSDGIGNSQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPTPLKAYNLTVADWHTYFVKGSQAETEGVWVHNSCPPKRTGSSKNEKHGDGGRSQIS
                     AESRIAELKNKIIPGMPKNERLKIEKTIKNITKNANRKAKGEEHGRRGR"
     gene            complement(3039..3356)
                     /locus_tag="ACFNML_000932"
     CDS             complement(3039..3356)
                     /locus_tag="ACFNML_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392341.1"
                     /translation="MNILPSWLRVGMNIAMLGMIHSDIRLITVDYEEGRRFLKIKNYL
                     SREAITEDHEDMEYLITELWSMCGEYFDEADFECIYSNHSSMELNQINGAVFRRKELI
                     SQA"
     gene            complement(3359..4627)
                     /gene="mafB"
                     /locus_tag="ACFNML_000933"
     CDS             complement(3359..4627)
                     /gene="mafB"
                     /locus_tag="ACFNML_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HFC6392342.1"
                     /translation="MKPLRRLTNLLAACAVAVAAFGQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQANINGTIGYHTRFSGHG
                     HEEHAPFDNHAADSASKEQGNVDEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEAVDQWMQENPNAAETVETLVNVLPFAKVKNLT
                     KAAKPGKAAVSGDFSDSYKHNTASRLSQSVDGEMFQTRNVDFKAKSIGTKIHDGAQGK
                     HISGHRNYIEGKSTLNQNINPQELLNGIHSGAYPVISKGARGNPVVDFGYPIGSDGKS
                     GLSTNFGTIHSGKNGVHIVPANPKTIKKVQ"
     gene            complement(4631..5572)
                     /gene="mafA"
                     /locus_tag="ACFNML_000934"
     CDS             complement(4631..5572)
                     /gene="mafA"
                     /locus_tag="ACFNML_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6392343.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKSDALSSVTTSTSLLNAPAAALTRNSGRKGERSAGLSVNGMGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGTVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWTGPYKVSKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            complement(5691..6791)
                     /locus_tag="ACFNML_000935"
     CDS             complement(5691..6791)
                     /locus_tag="ACFNML_000935"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFC6392344.1"
                     /translation="METQLYIGIMSGTSMDGADAVLVRMDGGKWLGAEGHAFTPYPDR
                     LRRQLLDLQDTGTDELHRSRILSQELSRLYAQTAAELLCSQNLAPSDITALGCHGQTV
                     RHAPEHGYSIQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHKALFRDDKE
                     TRAVLNIGGIANISVLPSDAPAFGFDTGPGNMLMDAWTQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFARPHPKSTGRELFALNWLETYLGGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAANARQMYICGGGIRNPVLMADLAECFGTRVSLHSTADLNLDPQWVEAAAFA
                     WLAACWINRIPGSPHKATGASKPCILGAGYYY"
     gene            7116..8690
                     /locus_tag="ACFNML_000936"
     CDS             7116..8690
                     /locus_tag="ACFNML_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HFC6392345.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            complement(9043..10464)
                     /gene="hemN"
                     /locus_tag="ACFNML_000937"
     CDS             complement(9043..10464)
                     /gene="hemN"
                     /locus_tag="ACFNML_000937"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFC6392346.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            10669..11403
                     /gene="fnr"
                     /locus_tag="ACFNML_000938"
     CDS             10669..11403
                     /gene="fnr"
                     /locus_tag="ACFNML_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFC6392347.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            complement(11516..12466)
                     /locus_tag="ACFNML_000939"
     CDS             complement(11516..12466)
                     /locus_tag="ACFNML_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HFC6392348.1"
                     /translation="MKLQQLKYALEVYQHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIARMVTSGEADLAIVTERIDDHPELGK
                     LPCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            12830..13558
                     /gene="rmpM"
                     /locus_tag="ACFNML_000940"
     CDS             12830..13558
                     /gene="rmpM"
                     /locus_tag="ACFNML_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HFC6392349.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLSRTNVQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
BASE COUNT         3093 a         3194 c         3656 g         3854 t
ORIGIN      
        1 aaaataggtt gtctattgtc aatcaaatat tcagccactc ctgatagaaa agctgcaata
       61 ttttctttat tccattcttc agaacaggta aaaattaatt caaacctact tttatagttc
      121 aagtcatatc gattcaagcc taaagtagaa aaagtagtta cccctttata tggttgtgat
      181 gggaagacgg aaacgctgag atgataacgt cgatccaagc attttatacc gttttcgatt
      241 tcacccaaat aatgctctaa atgttcgact gtaatcattt tttcttaaac ctttcatcct
      301 tgttgccacc agatcgattg caatgtctgc attctaaact tactccttta ttataatcat
      361 ttagttgctc cttacgatca gaactatgtt gacggttact ccatgatgga ttatgacttc
      421 catcccaacc atcttttgta ttacgtttct ccttgttatg gggattacca tatacatctt
      481 taccacatgt aggacatttt tttgaattag gctggctacc atctgctgcc aaatcccatg
      541 cattttttag cgtttcttgt ctgaaggttc cacgtttagc agtatttctc tcactgccac
      601 ttttaccttt tgcgctagaa ctaccatacg gacaatcatt atgaacccaa accccttccg
      661 tttccgccct atcacccttg acgaagtaag tatgccaatc ggcaacggtc agattgtagg
      721 ctttgagcgg ctgctgtttg agggtaatgt tttgaaccgt ctgttttgca ccgctttcgg
      781 aaagcagggt gtcgcctttt ttcagacgac ctgcctgtat ccattttcct tgactgtaaa
      841 acggatggat gctgttggaa atcagggttt ggttgttgcc gatgccgtct gaaatttcaa
      901 tgtaaacggt ttcctggtac ggattgccgt atcgggtggt aacgggtttg tatcctgttt
      961 ttccgcttgc ctcgtccttg gcaaagacgc ggtcgccgac tcggatacgg gcaatggctt
     1021 tgtagccgtc tgccgttttg accaaggtgc tgccgtggaa ggagcaggtg taggattttt
     1081 aaagaactta gttctcaata tcctgtttca ttcatcaaaa tgccgtctga aagctgaata
     1141 ccgcttcaga cggcattttg gtggttgggt ttttaagcca acctacgctt actgaaaacc
     1201 aaattgagtt tcagacagtt tttaggtttg ggtgtccaat ctaacttata tttgtccatt
     1261 taattagtcg tttgtatcaa atttccatta tttattttcc aatgtacttt ataactatct
     1321 tcataataat ctaattcaaa aaaacctgat atttcaatat ccaattccat tattgtttta
     1381 atgcattttt caaaataaat aatgaaataa gattttacgc atgcaccaaa aaaaatatag
     1441 ctgctccaat taaaactatt tgtcgggaaa acccacccgc ttttatatat ttttgcagat
     1501 tctttctctt cgatattgaa gggacaatta ttccaaaaat tattaatgtt ttcttggatg
     1561 atttttatat catcgtcaga gcattcaatc catcctttta tggaaacata tgatgccatg
     1621 tttaatctcc taaacctgtt ttaacaatgc cgccttttga ttcaatatat gacttaactt
     1681 gtgaatgaac accgtgcctg ttccgtacag caagcacagg ttggattaag attcgacaat
     1741 ctgtttggat tgacagaaag gtcgtctgaa aaatcgatta cggtttcaga cgaccttttg
     1801 ttgttgagtt ctcaacccaa cctatgcctg ccattaatgt tattcatcgt agtaaggttc
     1861 tgtcgaataa ttgtctttgc ccccggcaat gatagtaaca attttccctt ttgcttccca
     1921 agcttgtact cctatttcat caaactcata gacatatgtc ggataagatt catttgataa
     1981 ataatattta tcaacaccgt atgatttagg gtgatggaaa agctgtttaa aatcttcaaa
     2041 attcagacct attatattaa cgcccataaa atatagctcc tgataacaaa atatcgaaat
     2101 aattttgttt ttcttgacgg caattagtaa atttgagtcg ggagattcat aatattctgt
     2161 tctaaactca tcaggaggtt catacataaa agttcccagt atgtttttgt actgtgttat
     2221 atccgcacca aaacggaata ttcctacaga agtaaaaggt aaaaattcgg gagttttaac
     2281 gaccgcgtcg accatgctct tctccttttg cttttcgatt ggcatttttt gtaatatttt
     2341 tgattgtttt ctcaatcttt aagcgttcat ttttgggcat tccgggaata attttatttt
     2401 ttaattcagc aattcttgat tccgctgata tttgacttcg accgccgtct ccatgttttt
     2461 cattcttgga gcttcctgtt cttttaggcg gacaagaatt atgaacccaa accccttccg
     2521 tttccgcctg actgcccttg acgaagtaag tatgccagtc ggcaacggtc agattgtagg
     2581 ctttgagcgg tgttggtttg acaacgatgt tgcggacggt ttgggttttg ccactttcgg
     2641 ataacagcct gcttcccgct ttcaaatctt ccgctttaat ccatttgccg cccgaataaa
     2701 acggatggat gcggttggaa atcagggttt ggctgttgcc gatgccgtct gaaactttaa
     2761 tgtaaacggt ttcttgatac ggattgccgt atcgggcggt aacgggtttg tatcccgttt
     2821 ttccgcttgc ctcgtccttg gagaagacgc ggtcgccggt tcggatacgg gcaatggctt
     2881 tgtagccgtc tgccgttttg accaaggtgc tgccgtggaa ggagcaggtg taggattttt
     2941 gaagaactca gttctcaata tcctatttca aatgaaggaa atgccgtctg aaaattaaac
     3001 acggtttcag aagacattgt gattgttggt tttttaacct acgcttgcga aattaattcc
     3061 tttctcctga atactgcacc atttatttgg tttaactcca tagaagaatg attagaataa
     3121 atacattcaa agtcagcttc atcaaaatat tctccacaca tagaccataa ctctgtaatc
     3181 aaatattcca tatcttcatg gtcttctgtg atggcttctc ttgataaata atttttgatt
     3241 tttaaaaacc ttcttccttc ctcgtaatct acggtaatta acctgatatc tgagtgtatc
     3301 atgcccagca ttgctatatt cataccgact cgcagccagc ttggtaatat attcataact
     3361 attgcacctt tttaatggtt ttagggttag ccggaacaat gtgaactcca tttttacctg
     3421 aatgaatcgt accaaaattg gtacttaatc ctgatttccc atcgctgcct ataggatacc
     3481 caaaatcaac aacaggattt cctcttgctc ctttagaaat aactggataa gcacctgaat
     3541 gtattccgtt caacaattct tgaggattaa tgttttgatt taaagtactc ttaccttcaa
     3601 tgtagtttct atgtcctgaa atatgtttcc cttgagctcc atcatgaatt ttagtcccaa
     3661 tagattttgc tttaaaatca acattgcggg tttgaaacat ttctccatct acagactgag
     3721 ataatcttga agcagtgtta tgcttgtagg agtctgagaa atccccacta accgcagcct
     3781 tccccggttt tgccgccttt gtcaggtttt tgactttggc aaacggcagg acgttgacca
     3841 gggtttcgac ggtttcggcg gcattggggt tttcctgcat ccactggtca acggcttcgc
     3901 ccgcgctgat aaaggggtta agcgcgccgg cggcgacgcc gttgataaac tccatgctgt
     3961 tgccccagcg gtcgagcttg gcattgtgct cgaacatttt tctgttggct tcatcggcgc
     4021 ggtcggagaa attgctgccg aggttgttgt agttgtcgga tatgcgttgc cggatgctgc
     4081 gggtgtcggt cggattgagt ttgatgctgc gtgctgtgcc gttgacgtga taggtgtatt
     4141 cgtcgcgtgc gcccgtaggt ttggggtaat tgccgccctt cgggccgtcg taggcatcgg
     4201 cgggatggtg ctcgtgtcct tcccagttga gccggtacac ggtaaagcct tcgtcaacgt
     4261 tgccttgttc tttgcttgcg ctgtcggcgg cgtggttgtc gaagggggcg tgttcttcgt
     4321 gtccgtgtcc ggaaaagcgg gtgtggtagc cgattgtgcc gttgatgttt gcctgttgga
     4381 tgagcaggtt gcccatctgg tgggtatagt cttggatgac gttgattttg ccggtgcggt
     4441 cggaaacgct gccgcgcggg tcgccgaaga ggtggtattt gccgccgggt tcgtagtgct
     4501 gccgttgggc gttatcggta atgaacgggt cttgcgccaa gtccgccgcg agggcgggct
     4561 gtccgaaggc ggcgaccgct acggcgcagg cggcaaggag gtttgtcagt ctgcgcagcg
     4621 gtttcacggt ttatcctcct ttgcggcggc ggatgacttc gttgccgaca tcggggtttt
     4681 taccgttgtt ttgtttgaag tcgggacggt tttgggcggt tgtgtcgccg tagggggtga
     4741 tgtcggagaa atcgaccatc aggcggtctg aggctttgac ggttttgctg actttgtaag
     4801 ggccggtcca aagggcgtat tgttcttggt attgggattc gtaggcggcg gttttagggg
     4861 caatcagcag tttccggctg tcgcggtcga cggcgaaata ttcgagcttg gtttgggctt
     4921 taagggtttc ggcgttgtag aggtgcagtt cggtacggct gcggacggtg ccgaatacgt
     4981 cgacggttac gaatacgtcg gtgtcggcgt attcgggcgg tacgacttcg atgccgcgca
     5041 ggtagaagac ggtttggatg aggttggtca ggaaggaaac gtcgcggggg ttggcgagca
     5101 gggtttcgtt gcggtagtcg cccatgccgt tgacggacag tccggcggag cgttcgcctt
     5161 tgcgtccgct gtttctagtc agggcggcgg cgggggcgtt caaaagcgat gtggaagtgg
     5221 ttacgctgga gagcgcgtcg gatttggtgg tggcggtagt gtcgtaggcg gggtagctgt
     5281 attgggtggc actttcgggg ttgttgtggt agccaccgcg tatcagtgcg tcgatagagt
     5341 agcgtccgcc gcttatgttg cccgaacctt ggtcgcccat aacggagacg taaagggcgg
     5401 ctttgcgtcc tttcagggcg gacaaatcca tttctttgac ggcggcgcgg gacgatgcgg
     5461 cgacgagttc ttgttcgacg gcaaagcgtt tgccgccgcc gtgggcgggt atgccggtca
     5521 gtgtgccgca ggcggttagg acgaggggga tgaggaggag cagggttttc atggcagggt
     5581 ttgttttgtt ttgaacgagt ttgagtgtaa atggatttta agggtttgta aacgaaagag
     5641 gaaaaaatgc cgtctgagcg gcggaaatgg ctttcagacg gtatttgcgc tcaataataa
     5701 tatcccgcgc ccagaataca cggtttggat gcgccggttg ctttgtgcgg actaccggga
     5761 atgcgattaa tccaacacgc cgccaaccac gcaaatgcgg cggcttccac ccattgcgga
     5821 tcgaggttca ggtcggcggt gctgtgcagg gaaacgcgtg tgccgaaaca ttccgccaaa
     5881 tccgccatta aaacaggatt gcggataccg ccgccgcaaa tgtacatttg acgggcattt
     5941 gccgctgcgt gtgagacggc gtcgaaaacg gtttgcgcgg taaaacggga aagcgtccgc
     6001 aatacgtcgt atcggttttc gccgccgcca aggtaggttt cgagccaatt tagggcaaac
     6061 agttcgcgcc ccgtgctttt ggggtggggt cgtgcgaaat acgggtgggc gagcagcctg
     6121 tcgagcagtt gcggcaatat gttgccttgt gccgcctttg caccgttttt gtcgtaagga
     6181 agctgccagt gtgcctgcgt ccacgcgtcc atcagcatat tgcccggccc tgtgtcgaag
     6241 ccgaaggcgg gtgcgtcgga ggggagtacg ctgatgttgg caatcccgcc gatgttcagt
     6301 accgcgcgtg tttctttgtc atcgcggaac agggctttgt gaaaggcggg gacgagcggc
     6361 gcaccttgtc cgccggccgc aaggtcgcgg ctgcggaagt cgccgacggt aaaaatctga
     6421 gtcagttccg ccagcagcgg caaatcggca agctgtatgc tgtaaccgtg ttccggtgca
     6481 tgtcggacgg tttgcccgtg gcagccgagg gcggtaatgt cggacggcgc gaggttttga
     6541 ctgcacagca gttcggcggc ggtttgcgca tataggcggc tgagttcttg cgacaaaatc
     6601 ctgctgcggt gcagttcgtc tgtgcctgtg tcctgcaaat ccagcaattg gcggcgcagc
     6661 ctgtcgggat agggggtaaa ggcgtgccct tccgcgccca gccatttgcc gccgtccatc
     6721 cgtaccagca cggcatccgc cccatccata ctggttcccg acatgatgcc gatgtaaagc
     6781 tgtgtttcca tcatcactcc caaactggtg caaaacgcca ttttaacgtg tattgacgct
     6841 cgtataccga tttgccgccg cagtgtaaat aaagtgtaaa taaatgtttc aagacggatg
     6901 gaaaaatatt ataatgcgcc cgcaacatcc agtagtagaa gtgtcataca aaccgtttcc
     6961 ggcagcagtt ttgcattcgg tcaggtttgg gggtattcgg atgcggttca gaaggaagga
     7021 tgcgtctgcc atatcccgaa acggcagttc gaccggaggc agcagtacag tgtcggcaac
     7081 actcatgatt tccaccacat taaaggaaga ttgccatggc tcaaatccaa atgagcgcaa
     7141 atgttaaaac catcaacgcc gtctttgccg ccatgctggt aggtacagtc ggctatttta
     7201 tttattgggg cttgggttat acccattaca attacgccgc cttattcatt attgccacga
     7261 tgttcggcgt gtttatggcg ttcaacatcg gcggcaacga tgttgccaat tctttcggca
     7321 ccagcgtcgg tgcgggtacg ctgaccatcc cgcaggcttt gctgattgcg gcggtatttg
     7381 aggtcagcgg cgcggtcatc gcgggcggcg aggtaaccaa taccatacgc aaaggcatcg
     7441 tcgatttgaa gggtgttgat ttcgaaccca tacagtttgt gtttattatg atgtccgcgc
     7501 ttttggcggc ggcgttgtgg ctgttgtttg cctcgaaaaa agggcttccg gtatctacca
     7561 cccattccat tatcggcggc attgtcggca gcgcggtatg tatggcggta atgaacgatg
     7621 ccgcatcggg cgatttgata cgttggggca agctgggcgg tattggtgtt tcttgggtat
     7681 tgtcgcccgt gttgggcggc gcggtgtcct atttcctgtt ttcgcgcgtc aagaaaaacg
     7741 tcttagatta caacgcttgg gcggaaggca cgctcaaggg catcaagcag gaaaaaaagg
     7801 cctataaaga acggcaccgc ctgtttttcg agggtttgtc cgaagccgaa aaagtcgagt
     7861 acgccaccaa aatggcgcac gacgcgcaaa tttacgacga acccgaattc gatccgcaag
     7921 agctgcaatc ggagtattac cgcggtcttt atgcgttcga caaccgtaaa aacaatgtcg
     7981 attcctacaa agcgctgcat tcttggattc cctttatcgc ttcgttcggc gcgatgatga
     8041 tttccgctat gctgattttc aagggcttga aaaacctgca tttggggatg agcaacgtca
     8101 acagcttcct gaccatcttt atgataggcg cggcggtgtg gatggggacg tttgtgtttg
     8161 ccaaaagcct caagcgtaaa gacttgggca aatcgacctt tcagatgttt tcatggatgc
     8221 aggtctttac cgcctgcggc ttcgcattca gccacggtgc gaacgatatc gccaacgcca
     8281 tcggtccgtt tgccgcgatt atggatgttt tgcgtaccaa cagcgttgcc gcgcaaaatg
     8341 tcgtcccccc gattgcgatg ctgactttcg gcatcgcgct gattgtcggt ttgtggtttg
     8401 tcggtaaaga ggtgattaaa accgtcggta cgagtttggc cgaaatgcac cccgcctcgg
     8461 gttttaccgc cgaattgtcc gccgcatccg tcgtgatggg cgcgtcgctg atggggctgc
     8521 ccgtgtccag tacgcatatc ttggtcggcg cggtactcgg catcggtctg gtcaaccgca
     8581 atgccaactg gaaactgatg aagcccatcg gtttggcgtg ggtcattacc ctgcctgccg
     8641 ccgccgtatt gtcggttgtc tgctacttgg ttttacaggc agtattctga ttgtaaaata
     8701 ctgatgccgt ctgaacccgt gttcagacgg cattttgttg atggaatgtg cgggcttgtg
     8761 ccttatgcac aatctgttct gtcgggatat gccgtttggt atagtgatta acaaaaatca
     8821 ggacaaggcg acgaagccgc agacagtaca gctagtacgg caaggcgagg caacgctgta
     8881 ctggtttttg ttaatccact atatcttggt ttcggaacgg tcggacacaa aggtgcggaa
     8941 cgttatgata tgccgccgcc tgttcttgaa aacacttatc ctgccggcag caaaatgccg
     9001 tctgaaaaag cctttcagac ggcatttgta cgttagacgc aatcacaccg tttgcgaata
     9061 tttcgccttg gtttctttat ggcgcaggtg gtaatcgaac accatggcga tgttgcggat
     9121 gaggaagcgt cctttcgggg taacggtcag cccgtggctg ttcaggcgca ccaatcccaa
     9181 accggcgagt ttttccaaat ccgccagttc gtctttgaag tagcggtcga acgggatgcc
     9241 gaacatactt tcgtaaatcc ggtagtcgag cgcgaaacgg cacatcaaat cctgaatgat
     9301 gttgcggcgc aggatgtcgt cctgattgag ctgataaccg cgcatgatgg gcagtctgcc
     9361 ttcgtcgatg gcggcatagt aggcatcgat gtcgcgttcg ttttgggaat aggtgctgcc
     9421 gattttgccg atggacgaca cgccgatggc gaccaaatcg caatccgcgt aggtcgaata
     9481 gccttggaag ttgcgctgga ggaagccttc tttgagggcg atggagagtt cgtcgtcagg
     9541 tttggcgaaa tgatccatgc cgatgaagac gtagccgcgt tcggttaggg tttggacgca
     9601 gtattgcagc atatcgagct tctcttcgct gtcgggaacg gcggcggtat cgatgcggcg
     9661 ttgcggtttg aacacgtgcg gcaggtgggc gtagtgataa agggcgaggc ggtcgggatc
     9721 gagcgacaaa acggtatcga tggtggtttt gatgctttcc gaagtctggt gcggcaggcc
     9781 gtaaatcaaa tcgacgctga cggatttgaa ccccgcttcg cgcgccgcat cgatgacttc
     9841 tttggtttct tcgtaacttt ggatgcggtt gaccgccgcc tgcactttgg ggtcgaaatc
     9901 ctgaatgccg atgctcatgc ggttgaagcc gagtctgccg agcatgagga cggtgtcgcg
     9961 gctgactttg cgcgggtcga tttcgatgga gtattcgccg gtggggatta actcgaaatg
    10021 tttgcgtatc atgcggaaga cacgttcgat ctgttcgtcg ctcaaaaagg tcggcgtgcc
    10081 gccgccgaag tgcagttggg caagctggtg ccgtccgttc agatgtggag cgagcagttc
    10141 catttctttt tcaagatatt cgatgtaggc atcggcgcgg cttttgtctt tggtgatgat
    10201 tttgttgcag ccgcagtagt agcagatggt gttgcagaac ggaatgtgaa tgtaaaggga
    10261 aagcggtttg tttaacgcgc ccataccgcg caaatgtaaa gctttgatat attcgccttc
    10321 gcggaaaccg tcatggaaac ggtcggcggt agggtaggaa gtgtagcgcg ggccgctggc
    10381 gggcaggctg gcaatcagcg cgcggtcaaa ctcggggcgg tcatcgttta cattgtgatt
    10441 gttctgtatc tgaatgattt tcatggtgtg tgtgtgcgat tttatgatgt tagtcaaatt
    10501 ttggatagtt tggtagaatg ccacagtatg ataaacctgt cttgatatgt gtcaataagc
    10561 acatatagtg gattaaattt aaataaggac aaggcgaggc aacgccgtac tggtttaaat
    10621 ttaatccact ataatcatga tggggcaaag cgcacaaaaa ggtacggtat ggcttcgcat
    10681 aatactacac atcagatgaa aacgctgtgt tcttcctgtt ctttgcggga actctgcctg
    10741 cctgtcgggc tgctgcccaa cgagctcagc caactcgatg ccgtcatccg tcaaagccgc
    10801 cgcctgaaaa agggcgaata cctgttctgt gtcggcgaag cctttacctc gctctttgcc
    10861 atccgttcgg gcttcttcaa aacaaccgtc gccagtcagg acggccgcga tcaggtaacg
    10921 ggtttcttta tgtcgggcga actcatcggc atggacggca tctgttccca tgtgcacagt
    10981 tgcgacgcgg tcgccttgga agacagcgaa gtgtgcgaac tgccgtttac ccacatcgaa
    11041 gaactggggc aaaacatccc cagcctgcgt acgcacttct tccgcatgat gagccgtgaa
    11101 atcgtgcgcg accaaggtgt tatgctgctg ttgggcaata tgcgcgccga agagcggatt
    11161 gccgccttcc tgctgaacct ctcccaacgc ctttattccc gtggttttgc tgccaacgat
    11221 ttcatcctgc gcatgtctcg tgaagaaatc ggcagttatc tcgggctgaa acttgaaacc
    11281 gtcagccgca cattatccaa gttccatcag gaaggattga tttccgtcga gcataagcac
    11341 atcaaaatcc tcaatctgca ggtgttgaaa aaaatggtgt ccggctgctc gcacgccatt
    11401 tgattaaccc gtacgaacat ttcagacagc attctcaata aacacagggc agacgaaaac
    11461 atctgtcctg tttgttgtat ctgccgcaaa gtgccgtctg aaaaccggca gccgcctaaa
    11521 tcgaaaaatc ctcgctgatg ggcgtgtaca gaatcctatc caccttctcg cgtgtcaggt
    11581 gcggcgcaaa cgcttggata aagtcgtagg catatccgcg caaataagta tcgctgcgca
    11641 aagcaatcca cgtcggcgac ggctcgaaca ggtgtgccgc atccacaagc tgcaaatcgc
    11701 cgtccgtatc cgggttgtac gccattttcg ccatcagtcc cacgcccaaa cccaagcgca
    11761 cataagtctt caatacgtcc gtatccgccg ccgccaatgc gacatccggc tgttccaaac
    11821 gggctttgga aaatgcccgc gcgatgctgc tgcccgcatt gaatgcaaat tcataagtaa
    11881 tcagcggaaa cctcgccaaa tcttcaatgc ggagggggtt tctgcattcg agcaaggggt
    11941 ggtcgttcgg tacgataacc gcatgagtcc agtcatagca gggaagtttt cccagttcgg
    12001 gatggtcgtc tatccgttcc gtaacaatcg ccaagtccgc ctcgcctgag gtaaccatac
    12061 gtgcgatggc ggcagggctc ccctgtttga tggtcaggtt gactttcgga tagcgtttca
    12121 caaaatcggc aacaatcaag ggtagggcat agcgtgcctg agtatgcgtc gtggcaaccg
    12181 tcagcgaacc gctgtcctgt ccggtaaact cgctgccgat atttttaatg ttctgtacat
    12241 cgcgtaaaat acgttccgca atatccaaaa ccaccttgcc cggctgcgag accgaaacca
    12301 cgcgcttgcc gctgcggata aaaatctgaa tgccgatttc ttcttccagc aatttgattt
    12361 gtttggagat gccgggttgc gaagtaaaca aggcttcggc cgcttcggaa acgttcaggt
    12421 tgtgctggta aacttctaag gcgtatttca attgttgtaa tttcatggcg ggtcggtgtg
    12481 ggtctgtgtc gggtggctga acattgttta taatttatca tattttcttg ccggtacggt
    12541 atggggcttt gccgttgtgt ttgttgtttt tgtgcaacgg caatcgtgcg atatggaaaa
    12601 aatcccccta aagtaatgac acggaattga tttttcggca tgatagacta tcaggaaaca
    12661 ggctgtttta cggttgtttt caggcgttga gtattgacag tcctccccct gcttctttat
    12721 agtggagact gaaatatccg atttgccgcc atgtttctac agcggcctgt atgttggcaa
    12781 ttcagcagtt gcttctgtat ctgctgtaca aatttaatga gggaataaaa tgaccaaaca
    12841 gctgaaatta agcgcattat tcgttgcatt gctcgcttcc ggcactgctg ttgcgggcga
    12901 ggcgtccgtt cagggttaca ccgtaagcgg ccagtcgaac gaaatcgtac gcaacaacta
    12961 tggcgaatgc tggaaaaacg cctactttga taaagcaagc caaggtcgcg tagaatgcgg
    13021 cgatgcggtt gctgcccccg aacccgagcc agaacccgaa cccgcacccg cgcctgtcgt
    13081 cgttgtggag caggctccgc aatatgttga tgaaaccatt tccctgtctg ccaaaaccct
    13141 gttcggtttc gataaggatt cattgcgcgc cgaagctcaa gacaacctga aagtattggc
    13201 gcaacgcctg agtcgaacca atgtccaatc tgtccgcgtc gaaggccata ccgactttat
    13261 gggttctgac aaatacaatc aggccctgtc cgaacgccgc gcatacgtag tggcaaacaa
    13321 cctggtcagc aacggcgtac ctgtttctag aatttctgct gttggcttgg gcgaatctca
    13381 agcgcaaatg actcaagttt gtgaagccga agttgccaaa ctgggtgcga aagtctctaa
    13441 agccaaaaaa cgtgaggctc tgattgcatg tatcgaacct gaccgccgtg tggatgtgaa
    13501 aatccgcagc atcgtaaccc gtcaggttgt gccggcacac aatcatcacc aacactaagg
    13561 ctaggcaata tcttgccgat gcatgaggtt agtggatttt gtaccaggta ctgttgcaat
    13621 attcgtgaaa cgtcggtcgg catcgatgat gtgaaacaaa cccccgcttt tgcggggttt
    13681 gtttttttgg gtggttttct gaaacggcta tcgtcagaat cggggtgcag gttcggattc
    13741 ggattcggat tcagattcag attcagattc agattcaggt ttgtgtccca ttgccgc
//
LOCUS       DAXHTR010000048        13667 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.048, whole genome shotgun sequence.
ACCESSION   DAXHTR010000048 DAXHTR010000000
VERSION     DAXHTR010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.048"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..216
                     /locus_tag="ACFNML_000941"
     CDS             <1..216
                     /locus_tag="ACFNML_000941"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392350.1"
                     /translation="AFAGMTGCKFLLPRGWIPVFTGMTKFQTAFKRYGCVNNGRVGFS
                     LPIPAILAVFAFLSFSLFLPFTPNPAN"
     gene            complement(336..671)
                     /locus_tag="ACFNML_000942"
     CDS             complement(336..671)
                     /locus_tag="ACFNML_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFC6392351.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFTQGL
                     PPGVGDFFR"
     gene            complement(751..2874)
                     /gene="dnaX"
                     /locus_tag="ACFNML_000943"
     CDS             complement(751..2874)
                     /gene="dnaX"
                     /locus_tag="ACFNML_000943"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFC6392352.1"
                     /translation="MAYQVLARKWRPKTFSDLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCQSCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYAPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAEQDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAREMAACAV
                     GFDNALGELAILLQHLALIQAVPNALAHDDPDSDILHRLAQTIGGEQIQLYYQIAVHG
                     KRDLGLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELQSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSESKTAEPVTNQENNDVPPWEDAPDEAQTAAGTT
                     AQASAKSIQTASEAETSPDNQVSKNKAADNETETSLSEVPSENPIQATPNDEAVETEA
                     FAHEAPAEPFYGYGFPDNDCPPEDGAEIPPPDWEHAAPADAEEENNADEGSNDENNTP
                     SAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARATAD
                     KKRLDKIRDTLAQAYGLQLTLQTQDWHDESGRETPAMQDKRVQAEDRQKAQALLEADP
                     AAQKILQAFGAQWQPESLELAANRP"
     misc_feature    complement(1646..1685)
                     /gene="dnaX"
                     /locus_tag="ACFNML_000943"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            complement(2969..4945)
                     /gene="mutL"
                     /locus_tag="ACFNML_000944"
     CDS             complement(2969..4945)
                     /gene="mutL"
                     /locus_tag="ACFNML_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFC6392353.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELAGGGIRLIRVSDNGSGIHPDDIELALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSEATEYAHCATMLERLALAHPHIAFSLKRDGKQVFKLPEQSLHERIAAIVGDDFQT
                     ASLEIDSGSGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVSNAGEVLHDITGVVSTPMPSENDSENLFDRASNYPTGNKSDTHNAFGSSGKTAPMP
                     YQSAYAPQQRSLSLRESRAAINTYAELYKKTDDIDLELSRFEQARFGNMPSETPAPQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQR
                     QENGRLQSQRLLIPVTFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            5088..5273
                     /locus_tag="ACFNML_000945"
     CDS             5088..5273
                     /locus_tag="ACFNML_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HFC6392354.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            5284..5952
                     /locus_tag="ACFNML_000946"
     CDS             5284..5952
                     /locus_tag="ACFNML_000946"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6392355.1"
                     /translation="MTTHLSNVAPDLQNYLNSIGEPEHPVLTRLREKTEHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEVGVFTGYSSTALALALPEHGRITACDINVTFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            5952..6668
                     /locus_tag="ACFNML_000947"
     CDS             5952..6668
                     /locus_tag="ACFNML_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HFC6392356.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAMFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            6716..7201
                     /gene="purE"
                     /locus_tag="ACFNML_000948"
     CDS             6716..7201
                     /gene="purE"
                     /locus_tag="ACFNML_000948"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFC6392357.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANETPELAQKLADFRAKQEQAVLNMELEQ
                     I"
     gene            complement(7384..8838)
                     /locus_tag="ACFNML_000949"
     CDS             complement(7384..8838)
                     /locus_tag="ACFNML_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFC6392358.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHVRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACEII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(8835..9536)
                     /locus_tag="ACFNML_000950"
     CDS             complement(8835..9536)
                     /locus_tag="ACFNML_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFC6392359.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREMGVSWGSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNIDAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPTDLEENA"
     gene            complement(9533..10312)
                     /locus_tag="ACFNML_000951"
     CDS             complement(9533..10312)
                     /locus_tag="ACFNML_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFC6392360.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVARAQLDLFPEEWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPNMPRPKHIASFLLERTGGKA"
     gene            10596..12002
                     /locus_tag="ACFNML_000952"
     CDS             10596..12002
                     /locus_tag="ACFNML_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6392361.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFILGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDIKGSNISLDFKGYLIFSAAACLLLLSA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTASKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FKRLHVSDGANLTRNTLS"
     gene            12114..13640
                     /locus_tag="ACFNML_000953"
     CDS             12114..13640
                     /locus_tag="ACFNML_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HFC6392362.1"
                     /translation="MKTRSLISLLCLLLCSCSSWLPPLEERTESRHFNTSKPVRLDNI
                     LQIRHTPHTNGLSDIYLLNDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIIRSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAGRLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
BASE COUNT         2786 a         3692 c         3825 g         3364 t
ORIGIN      
        1 gcgttcgcgg gaatgacggg gtgtaagttt ctgctcccac gaggctggat tcccgttttc
       61 acgggaatga cgaagtttca gacggcattt aagcggtacg gatgtgtaaa taatggtagg
      121 gtgggcttca gcctgccgat tcccgctatt cttgccgttt ttgcgttctt atcattctca
      181 ctgtttttac cgttcacgcc gaatcctgca aactgatgcc gtcattcccg cgaaggcggg
      241 aatctagacc cgtcggcacg gaaatttatc gagtaaaacg gtttcttaga ttctacgttc
      301 tagattcccg cctgcgtggg aatgacggtc gggtatcagc ggaagaagtc gcccactccg
      361 ggggggagtc cttgcgtgaa tgcgcccatt gttttgtttg cggtttcttc ggctttgtca
      421 cgggcggatt tgagggcggc gaggatgagg tcttcgagca tttctttgtc gtcggcggct
      481 tcttgaatca aatcggggct gatgtcgatt ttgcgtactt cgtgcgcgca ggtcattgtg
      541 attttgacca agccgttgcc tgcttcgcct tcgatttcgg tttcggcaag tttggcttgt
      601 gcttttttca tattttcctg catttgctgc gcctgtttca tcaggccgcc taatccggct
      661 tttccgaaca tactgaatac tcctgtctgt tttgattgaa taagggaaac ggcaatgccg
      721 tccgaagggt tcgggcggca ttatatctgt ttatggccgg tttgccgcca attccagtga
      781 ttcgggctgc cattgcgcgc cgaatgcttg gaggattttt tgtgcggcgg ggtcggcttc
      841 gagcaatgct tgtgcttttt gcctgtcttc ggcttggacg cgcttgtcct gcattgcggg
      901 ggtttctctg ccggattcgt cgtgccagtc ttgggtttgc agggtgagtt gcagcccgta
      961 ggcttgggca agggtgtcgc ggattttgtc gaggcgtttt ttgtcggcgg tggcgcgtgc
     1021 ttcggcggtc attgccaaaa ccatcaggcc ggtgtcggga tggtattccg tccacgcgga
     1081 gtgttgcgcc ggcatttgcg ccgcgccgag tttgcgggcg aaatgccgga cgatggctgc
     1141 ccagttttcg gtggaaaact cgggcggcgg ggcggacggc gtgttgtttt cgtcgttgct
     1201 gccttcgtcg gcgttgtttt cttcttccgc atcggcaggg gcggcgtgtt cccaatccgg
     1261 cggcgggatt tctgcgccgt cttccggggg gcagtcatta tccggaaagc cgtaaccgta
     1321 gaaaggttct gcaggggctt cgtgcgcaaa tgcttccgtt tcaacggctt catcattcgg
     1381 tgttgcctga atggggtttt cagacggcac ttcggacaag gaagtctcgg tttcgttgtc
     1441 ggctgccttg ttcttggaaa cttgattgtc aggcgatgtt tcggcttcgg atgccgtctg
     1501 aatgcttttt gccgatgctt gcgcggtcgt gccggctgcg gtttgggctt cgtccggcgc
     1561 gtcttcccaa ggcggaacat cgttgttttc ttgattggta acgggttcgg cagttttgct
     1621 ttcagacggc attgccgctg cggatgccgt ctgaacgggt gtttgggcgg tttccgcttc
     1681 agggcgcggt tggggctttt ttgcggcggt ttccttttcg gcggtttgtg ccgatgggga
     1741 ttgaagttcg gtattttcaa tcacggcgtt tgcatcacac gaagctgccg ccaagggcgc
     1801 aaacgccagc atacgcagca gggtcatcat aaagccggcg tattcgtcgg gggcgaggcc
     1861 caagtcgcgt ttgccgtgga cggcgatttg gtagtaaagc tggatttgtt cgccacctat
     1921 ggtttgggcg aggcggtgca aaatatcgga atcggggtcg tcgtgcgcca aggcattcgg
     1981 cactgcctgt atcagggcga ggtgttgcag cagtatggcg agttcgccca aggcgttgtc
     2041 aaagccgacg gcacacgccg ccatttcccg cgctttggcg gtcagggctg cgccgtcttg
     2101 gttgatgatg cctgtcagca gttcgtaaag gtattgtttg tcaaccgcac caatcatttg
     2161 gcggacatct tgttcggcaa ctttgcccga acccagggcg atggcttggt cgagcaggct
     2221 caaggcatcg cgcatcgatc cggcggcggc gcgtcccaaa agttgcaggg cggggggggc
     2281 gtaggcgatt ttttcgctgt cgaggacgtg ggcaaggtgg tcggcaacct gctgcgcggt
     2341 catattgcgt aagacgaatt gcaggcagcg gcttaatacg gtaacgggga ctttgtgcgg
     2401 atcggtcgtg gcaaggatga atttgacgtg ttcgggcggc tcttccagcg ttttgagcat
     2461 agcgttgaac gcgcttttgg aaagcatatg cacttcgtcg atgatataga ctttgtattt
     2521 gccggcggtc ggcgcgtatt gggcgttttc caatacttcg cggatgttgt cgatgcctgt
     2581 gttggaggcg gcgtcgattt ccagcaggtc gacatagcgt ccggcatcga tctgcgtaca
     2641 gctttggcac acgccgcaag gttcgccgtg ttgcgcgttt tcgcagttga ggcttttggc
     2701 aaggatgcgg gcgatggtgg ttttgcctac gccgcgcgtg ccggtcagca ggtaggcgtg
     2761 gtgcagcctg ccttcgtcca aggcattttg cagggctttg acgacgtgtt cctgaccgac
     2821 taggtcggaa aaggttttgg gccgccattt tcgggcgaga acttgatagg ccatgttttt
     2881 ctcttggttt cggtcgtgat gtttctgtcg gtgcgtcgga atgccgtctg aacggcggtc
     2941 tcgggcggcg tattctagca ctttcggctt actgtccgcg caagaacagt gcgtccaatt
     3001 ctttcaaagt cagtttgacc caagtcggcc tgccgtggtt gcactggttg ctgcgcggcg
     3061 tattttccat atcgcgcaga agggcgttca tttcgggcag ggtgagccgg cggccggcgc
     3121 ggatcgagcc gtggcaggac atagtggcga ggatgcggtt ttcgtgttct tcgatggttt
     3181 ggctgctgcc gacttgggcg agttcgccca atacgtcgcg ggcgagggag acgacatcgg
     3241 atttgcccaa cattgcgggt gcggcgcgga cggcgagggt gttgccgccc atatcggata
     3301 attccagccc gaagcctgcc agcgtttcgg cataatcggc aagggcggcg cattcttcgt
     3361 gggacgcggc aaaggttacg ggaataagca ggcgttggct ttgcagcctg ccgttttcct
     3421 gccgttggcg tttcattttc tcgtagttga cgcgttcggc ggcagcgtgc atatcgatga
     3481 gcaacaggct gtcttcggct tgggcaagaa tgtagatgcc aagtaattgg gcaatggcaa
     3541 aaccgagcgg cggcagttcg gattgggacg ggatgccgtc tgaaagcggc gtatctgttt
     3601 ggggagcagg cgtttcagac ggcatattgc cgaaacgtgc ctgctcgaat cggcttaact
     3661 caaggtcgat gtcgtcggtt tttttgtaga gttcggcata ggtgtttatt gccgcgcggc
     3721 tttcgcgcag ggacaggctg cgttgttgcg gcgcatatgc ggactgatag ggcatgggcg
     3781 cggttttgcc tgatgaacca aaggcattgt gtgtatctga tttgttgcct gtcgggtagt
     3841 tggatgcgcg atcaaacaga ttttcgctgt cgttttcaga cggcattggg gtggagacaa
     3901 cgccggtaat gtcatgcaac acttcgcctg cgttgctgac gctttcggtc aagtcggcgc
     3961 gtgtgtcggc aagggcttta ttgagcgtgt ggaacacgag ttggtgcacc tgccggctgt
     4021 cgcggaagcg gatttcggtt ttggtcgggt ggacgttgac atccacggct tcgggcggca
     4081 ggtcgaggaa aaggacgaaa gcgggggtga gggcgttgtg caaaacgtcg cggtatgcct
     4141 gcttgacggc gtggagcatc actttgtcgc ggacaaaacg atggttgacg aagcagtatt
     4201 gtttgtcggt tttgcctttg gcgaaagtcg gcttggcaat cgcgccatag agccgcaatg
     4261 cgccgctgcc gctgtcgatt tccaaagatg ccgtctgaaa atcatcgccg acaatggcgg
     4321 caatccgttc gtgcaggctt tgttcaggga gtttgaacac ttgtttgccg tcgcgtttga
     4381 gcgagaaggc aatgtgcgga tgcgccagcg cgaggcgttc gagcatagtg gcgcagtggg
     4441 cgtattcggt ggcttcagac ttgaggaact tgcgccgcgc gggggtgttg aagaagagtt
     4501 cggcggcttc gatggtggtg ccgacggggt gggcggcggc ggtggggctg ctgagtttgc
     4561 cgtcttcggc tttgacttgg gttgcgtgcg aactgtcttc ttgacggctg gtcagggtca
     4621 ggcggctgac ggaggcgatg cttgccaagc cttcgccgcg aaagcccatg ctggcaacgt
     4681 gttctaaatc gtttaaggtt ttgattttgc tggtagcgtg tcggtggagc gcaagctcga
     4741 tgtcgtccgg atgaataccg ctgccgttgt cgctgacgcg aatcaggcgg atgccgccgc
     4801 ccgccagctc gacatcaatc gccgttgcgc ctgcatcgat actgttttca acgatttctt
     4861 tcaaggcgtt ggcagggcgt tcgaccactt cgccggcggc gatttggttg acaagatggt
     4921 cgggcagggc ggcaatgcgt ggcataaggt aggcttccgt tgcaaaaacg ctttattcta
     4981 taataaaacc ccttactttt ctgcctgtat tcttgataaa agccgtttat cccgtttgga
     5041 aaataccagt ataatcacgc cgtatcccgt aaaattggag cgcaaagatg tatcactacc
     5101 aatccgaagc cacacaattc ctcaaccgcc tgattgaaga aaaacctgag ttggaacagc
     5161 agcgtttgga aaaccggggg cttttgtggg atgtcgaact caatcccgaa gaacaggaaa
     5221 acttcgaggc ggcaaaagta gcgaaaaaac cttataccta ttaccaagac tgaacccgcc
     5281 gaaatgacga cccatctgtc caatgtcgca ccggacctgc aaaactatct gaacagcatc
     5341 ggcgaacccg aacatcccgt tttgacgcgg ctgcgtgaga agaccgagca tcaccgtatg
     5401 ggcaaaatgg cgattgcgcg cgaacaggcg gcggttttgg tttggctggc aaagctgatt
     5461 cgcgcggaaa aatatctgga agtcggcgtg tttaccggat acagcagcac cgcgcttgca
     5521 ttggcgttgc cagaacacgg gcggattacc gcctgcgaca tcaatgtaac ctttaccgat
     5581 acggcgcgtc aggtttggaa cgaggcaggt gtggcacata aaatcagcct gcacctgcaa
     5641 cccgcattgc tgacattgga tgatttgatt gcacagggtg aagccggaag ctacgatttg
     5701 gcactgatag acgcagacaa accgcccacg ccgcgatatt tcgagcgttg cctcaagctt
     5761 gtccgtcaag gcggcatcat cgccatcgac aatattttgc tgaacggaag ggtgatgcgc
     5821 gaagcggctt ccgatgcgcc gcccagcgtc ggcatcctca aagatttcaa tcaaaacctg
     5881 ccgaacgata cgcggattgt ccccatcacc ctgcccgtcg gcgacggctt gaccctgctt
     5941 ctgaaaaaat aatgaagacc aaattaccgc tttttatcat ttggctgtcc gtatctgccg
     6001 cctgttcttc ccctgtttcc cgcaatattc aggatatgcg gcccgaaccg caggcagagg
     6061 caggtagttc ggacgctatt ccctatcccg ttcccactct gcaagaccgt ttggattatc
     6121 tggaaggcac actcgtccgc ctgtcgaacg aagtggaaac cttaaacggc aaagtcaaag
     6181 cactggagca tgcgaaaaca cacccttcca gcagggcata cgtccaaaaa ctcgacgacc
     6241 gcaagttgaa agagcattac ctcaataccg aaggcggcag cgcatccgca cataccgtcg
     6301 aaaccgcaca aaacctctac aatcaggcac tcaaacacta taaaagcggc aggttttctg
     6361 ccgctgcctc cctgttgaaa ggcgcggacg gaggcgacgg cggcagcatc gcgcaacgca
     6421 gtatgtatct gttgctgcaa agcagggcgc gtatgggcaa ctgcgaatcc gtcatcgaaa
     6481 tcggagggcg ttacgccaac cgtttcaaag acagcccaac cgcgcccgaa gccatgttca
     6541 aaatcggcga atgccaatac aggttgcagc agaaagacat tgcaagggcg acttggcgca
     6601 gcctgataca ggcttacccg agcagcccgg cggcaaaacg cgccgccgca gccgtgcgca
     6661 aacgatagtt tttccatttt tccgcttttt accccatccg caccaaggag gatttatgat
     6721 acaaatcggc atcatcatgg gcagcaacag cgattggccc gttatgcggc aggcagcgca
     6781 gtttcttgaa gagttcggcg tagaatatga ggcgcgcgtt gtttccgcac accgcacccc
     6841 ggatttgatg ttccaatacg ccgaaaccgc acgggcgcgc ggcatcaaag ccatcatcgc
     6901 gggtgcgggc ggcgcggcgc atttacccgg tatggttgcc gccaagacca ccgtccccgt
     6961 tttgggcgta cccgtcccca gcaaatacct gcgcggcgaa gattcgcttt tatcgattgt
     7021 acaaatgccc aaaggcgtac ccgtcgccac attcgccatc ggcgaggcag gcgcggcaaa
     7081 tgccgcattg ttcgccgttt ccatgctcgc caacgaaacc cccgaactgg cgcaaaaact
     7141 ggcagacttc cgagccaaac aggaacaggc tgttttgaat atggaattgg aacaaattta
     7201 aaacccaccg tctgcgaata tatagtggat taaatttaaa ccagtacggc gttacctcgc
     7261 cttgccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc
     7321 actatacaaa tgccgtctga agccctgttt cgggtttcag acggcatttt tgcgaacaac
     7381 tttttatgcc tgttcttttt ggcgcatttt ttctgccatt agttcgtgca aggttttctt
     7441 cgccggtttc atcggcacgc ggttttgcgt ccaacccaac tgtttgcgtg gggtcaggtt
     7501 gcggaacttg gtggctgccc aaccgaaggc gcggtaggtt ttgctgccgc tgaaaatacc
     7561 gttgaatgtg cgccacgcca tttgttcgcc gaaggtatgc gatgcgcctt gccctcggat
     7621 ggggtgcggc acggtttcgg tcggcgaacg ttgcgcttca acacgcagac gttgcatttg
     7681 ttcggtaatc ggaatgcgta ccggacaaac ttcaacgcac gcgccacaca tagtacacgc
     7741 agtcggcaga tcgcgtgtcg catccaagcc caacaaatgc ggagagataa tctcgccaat
     7801 cgggccggga taagtcgtac cgtaagccgc accaccgata cgggtataaa ccggacagtg
     7861 gttcatacac gcaccgcaac ggatacattg caaggtgcgg cgcatttgat cttctgcgta
     7921 agcttggctg cggccgttgt cgagcagaac caagtgcatt tcttgcggac cgtctaattc
     7981 ttcactgcgg cgcgggccgg taatcatatt gaaataggtt gtgatgtttt gaccgatggc
     8041 ggagcgcggc agcagactgt acaagggtgg gatgtcggac aatttcgcca ccactttttc
     8101 aatgccggta atagcgatat gcacgggcgg tacggtggta ctcaagcgac cgttgccctc
     8161 gttttccacc agacacagcg tacctgtttc agcaacggca aagtttacgc cactcaaacc
     8221 gacatcggca gtgctgtaaa tatcgcgcag tgctttacgg gcgaagccgg tcagttggtc
     8281 tacatcgtct gtcagcggcg taccgaggtt ttggtggaac agttcgctaa cctgttcttt
     8341 ggttttgtgg atagcaggca tcacgatatg ggtcggtttt tcacctgcca tttggacgat
     8401 gaactcgccc aagtcgcttt ccaccgcttt aatgcctttt gcttcaagat aatggttcag
     8461 ctcgatttcc tcgctgacca tcgatttgcc tttgaccatc agcttgccgt ttttggctgt
     8521 gatgatgtcg tggatgattt cgcaggcttc ggccggtgtt tccgcccagt gtacttttac
     8581 gcccaacttg gtcaggtttt cttccagctg ctccagcagg gcaggcaatt tagacaatga
     8641 gcgctgacgg acgtgttcgc acaaatcgcg caagctttgc agctcttctt cgtcggtcaa
     8701 aacagctttg cgtttggtca tcagcatatc catcgcggta cgcaggcttt tacgcaaagg
     8761 cttgtcttga agggaaattg cggcgttttg cttgaaagtt tccggcttca tgtgaaattt
     8821 gatggtttgc gtagtcatgc gttttcctcc aaatcggtag gggaaatgtg gtcgggcagg
     8881 atggcgagga taaccaaatc acgcggaccg tgcgcgccgt aagcaagcgt cagttggatg
     8941 tctgcggttt tggacggacc ggaaatcagg aatacattgg tcggcatacc gttttccacc
     9001 agtttttcgc cttcgacggc attatgaaac tcgttgtaca tcttggacgt atcgaacagg
     9061 cagaaatgca cgggcggaac gagactcaaa gtacgcggct cttcggggct ggaaaacagc
     9121 atcagcgtgc cggtgcgggc gatgccgcat tgcgcgccgc tgaagcccgc atcgatgttc
     9181 gtgaaaaact cggttttcca agtatcgatt tcgcgctcga aggcaatcgg ttcgatattg
     9241 ctgtccgcca atgcggcacg ggcaatttgt ccgtgttcgg tcgccaaggg cagcaggatg
     9301 tttttcaaac ccttgccttc tgccgcttcg cggaaaacct gcatccaatt gcttttcgtc
     9361 acccaataaa tttcggtttt gaccgcgcgc attgcggcag cccaatgttt cagacgctcg
     9421 gcttcgctgc cccaagaaac acccatttca cggtaataat caaaaaccgc aggttcttcc
     9481 atcggcaatg cgtcggcttt tttcagtttt gccaaaatat tttcgcgcgc gctcatgctt
     9541 tgcctccggt acgttccaac aagaaggatg cgatatgttt cggacgcggc atattcggtt
     9601 cgtccttagc aattttgccg ccgatgttca tcatacagcc gcagtccgcg ctgatgattt
     9661 cggttgcgcc ggtttctttc agcgcggcga ctttgtcggt taccatcgcg ccggaaatat
     9721 cggcttgttt gacggagaat gtgccgccga agccgcaaca ttcgctttcg tggtcgtgga
     9781 cgatgcgttc gacgttttcc ataccgtcaa tcagttgcca gcccgagaga tggacattca
     9841 tttcgcggcg ggcggcgcag gaagtgtgaa cggcgacttt gagcggttcg cccttgtctt
     9901 cgggtttgaa accgatggca agcaggaaat gggtaaactc gatgatgcgg ccggcgcaat
     9961 ccacagccct ttcctcgtac tcgctgcctt taaacagcgt cggccaatgg tgcttcatca
    10021 taccgccgca cgaaccggac ggtacgacaa tcggccactc ctcggggaaa aggtcgagtt
    10081 gcgctctggc gacatcaaag gcttcggtcg gatggccgga tgaataggcg ggctgaccgc
    10141 agcagctttg cgccatcggg aaatggacgc gtatgccctg ttgctcgatt agggtaatgg
    10201 catccatgcc tgcttcgggc ataaaaagat caaggacgca agtgccgaaa aaatagacat
    10261 cggtcggatt gctgtcgtat cggatgattt ttgggggaat ggttgcgctc atgttttttg
    10321 ataacgggaa acccgttttt ttctgtagaa aggtaagcgt ttactttaag taattgactg
    10381 ttgcgggtca agtctaattt taaaaaataa tccggttttt cttacaaact gccccataac
    10441 gcttactgta ccttactctg ataattttcg ataataatta tcattacaat gaaatgccga
    10501 ttcgtttgct tgtgaacatt caggacgccg actctgacgg cattcagaca gcatctgaaa
    10561 acaataacgg cacaaggatg gggtacaatc gccctatgga aaaaaacacc ctatccgccc
    10621 gcgcaccatc cccttggctg ccgcttctgc tggcaattgc catttttatg cagatgttgg
    10681 atgcgaccat tttaaatacc gcactgcctg aaattgccac cgacctggac gaatcgcctc
    10741 tggatatgca actggcagtc gttgcctata cgctgacggt tgccctgctg attcctttga
    10801 gcggttattt ggcggacagg ttcggaacga aaaaagtctt tttcggttcg attgccgttt
    10861 ttatactcgg atcggcattg tgcgccgcat cgggttcgct gtttgaattg acgctttccc
    10921 gtgtcgttca gggcatcggc ggttcgatgc tggttccgat accgcgtctg accatcttgc
    10981 gtatgtacga caagtccaag ctgctcaatg ccatcaatta tgcggtcatg cctgcattaa
    11041 tcgggccggt tttagggcct ttggcgggcg gttatttggt cgaatacgct tcgtggcact
    11101 ggattttcct gctcaacctg cccatcggtc tgctgggttt catattggga cgcaacatca
    11161 tgcccgatat taaaggcagc aatatctctt tagacttcaa aggctatctg attttttctg
    11221 ccgccgcgtg cctcttgtta ctttcggcag aaagcctgtc gcacgcgctg cctccgtatt
    11281 ttgcactgtt gccgctgtgc ggcggactgc tgtttgcacg ccgttatttc cgacatatga
    11341 aaaccgcgtc caaaccgatt tattccgccg acctgtttct gatacgcact ttccgtctag
    11401 gactggcggg caatctgttc agccgtctcg gcatcagctc gattcctttt ctgatgcccc
    11461 tgatgtttca agtcgctttc ggcttcggcg caagcctgtc gggttggctg gtcgcccccg
    11521 tcgccctgtc ttcgctgctg gtcaaaccgc tgattgcacc gctcatgaaa cgtttcggct
    11581 accgcacggt actgctttgg aacaccaagc tgcttgccgc cttcatcatg ctgctcgccc
    11641 tgcctgacgg aaactcgtcg ctgtggattt ggattttcct ctcgctggcg atcggcgcgt
    11701 gcaactccct gcagttttct gccatgaata cactgaccct cgccgatttg cgcccgcaac
    11761 agaccggcag cggcaacagc ctgatggcgg tcaaccaaca gcttgccatc agcatgggca
    11821 ttgttgtcgg cgcattaatc cttaaaaact ggacatttct gataccggct tcttcaggtc
    11881 tgcattccgc cttccgtatg accctgctca gcatcggcgg catcaccctt gcctcgtcgt
    11941 tggttttcaa gcgtctgcac gtttcagacg gcgcaaacct gacacggaac acactgtcct
    12001 aaagcggtcc gcacgcaaaa cttttacccg tttcaacgtt tggattatga ttccgcacat
    12061 ccatgcgcgc caaccccaaa acacaggcaa tgccgtctga aaccatatcc ctgatgaaaa
    12121 cacgcagcct aatttccctt ttatgcctcc ttctctgttc atgttcttca tggttgcccc
    12181 cactggaaga acggacggaa agccgtcatt tcaatacttc caaacccgtc cgcctggaca
    12241 acatcctgca aatccggcac acccctcata ccaacgggct atccgatatc tatctgctga
    12301 acgaccccca cgaagccttt gccgcccgcg ccgcccttat cgaatctgcc gaacacagcc
    12361 tcgatttgca atactacatc tggcgcaacg acatttccgg ccgactgctg ttcaacctcg
    12421 tgtaccttgc cgcagaacgc ggtgtgcgcg tacgcctgct gttggacgac aacaacacgc
    12481 gcggattgga cgacctcctg ctcgccctcg acagccatcc caatatcgaa gtgcgcctgt
    12541 tcaacccctt cgtcttacga aaatggcgcg cactcggcta cctgaccgac ttcccccgcc
    12601 tcaaccgccg catgcacaac aaatccttta ccgccgacaa ccgcgccacc atactcggcg
    12661 gacgcaatat cggcgacgaa tacttcaaag tcggtgagga caccgttttc gccgacctgg
    12721 acatcctcgc caccggcagc gtcgtcggcg aagtatcgca cgacttcgac cgctactggg
    12781 caagccattc cgcccacaac gccacgcgca tcatccgcag cggcaacatc ggcaagggtc
    12841 ttcaagcact cggatacaac gacgaaacgt ccagacacgc gctcctgcgc taccgcgaaa
    12901 ccgtcgaaca gtcgcccctc taccaaaaaa tacaggcagg acgcatcgac tggcagagcg
    12961 tccaaacccg cctcatcagc gacgaccctg caaaaggact cgaccgcgac cgccgcaaac
    13021 cgccgattgc cgggcggctg caagacgcgc tcaaacagcc cgaaaaaagc gtctatctgg
    13081 tttcacccta tttcgtcccc acaaaatccg gcacagacgc actagcaaaa ctggtgcagg
    13141 acggcataga cgttactgtc ctgaccaact cgctacaggc gaccgacgtt gccgccgtcc
    13201 attccggcta tgtcaaatac cgaaaaccgc tgctcaaagc cggcatcaaa ctctacgagc
    13261 tgcaacccaa ccatgccgtc cctgccacaa aagacaaagg cctgaccggc agctccgtaa
    13321 ccagcctgca cgccaaaacc ttcattgtgg acggcaaacg catcttcatc ggttcgttca
    13381 acctcgaccc ccgttccgcg cgtctcaaca ccgaaatggg cgttgttatc gaaagcccca
    13441 aaatcgcaga acagatggag cgcacccttg ccgataccac ccccgaatac gcctaccgcg
    13501 ttaccctcga caggcacaac cgcctgcaat ggcacgatcc cgccacccga aaaacctacc
    13561 cgaacgaacc cgaagccaaa ctttggaaac gcatcgccgc aaaaatccta tccctgctgc
    13621 ccatagaaag tttattatag aaatatagcg gattaacaaa aaccagt
//
LOCUS       DAXHTR010000049        13666 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.049, whole genome shotgun sequence.
ACCESSION   DAXHTR010000049 DAXHTR010000000
VERSION     DAXHTR010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13666
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.049"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(78..278)
                     /locus_tag="ACFNML_000954"
     CDS             complement(78..278)
                     /locus_tag="ACFNML_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392363.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALREVAEISAWVLLG
                     AAAAMLFWYLFMLYFKHIPDSY"
     gene            428..1567
                     /locus_tag="ACFNML_000955"
     CDS             428..1567
                     /locus_tag="ACFNML_000955"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HFC6392364.1"
                     /translation="MSQNASVGIVMPQKIPFEMPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGRHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLGIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGNSLTRAVQDVQAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKECQQ"
     gene            1564..2145
                     /gene="metW"
                     /locus_tag="ACFNML_000956"
     CDS             1564..2145
                     /gene="metW"
                     /locus_tag="ACFNML_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HFC6392365.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTSNRQVKHFPNLLGSLAFYRVG"
     gene            2262..3098
                     /locus_tag="ACFNML_000957"
     CDS             2262..3098
                     /locus_tag="ACFNML_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NMB0938 family lipoprotein"
                     /protein_id="HFC6392366.1"
                     /translation="MKNKTSSLLLWLTAIMLTACSPNKNDKTKEVGASAASSSASSAS
                     SQSDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALSHHE
                     LETRFGLPGGGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDALME
                     QERRLREATLLLIQGSQETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYIGQL
                     PGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNPNLG
                     RR"
     gene            complement(3148..3708)
                     /gene="efp"
                     /locus_tag="ACFNML_000958"
     CDS             complement(3148..3708)
                     /gene="efp"
                     /locus_tag="ACFNML_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFC6392367.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(3751..4902)
                     /gene="earP"
                     /locus_tag="ACFNML_000959"
     CDS             complement(3751..4902)
                     /gene="earP"
                     /locus_tag="ACFNML_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0106361 - protein-arginine
                     rhamnosyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HFC6392368.1"
                     /translation="MNTYAFPVCWIFCKVIDNFGDIGVSWRLARVLHRELGWQVHLWT
                     DDVSALRALCPDLPDVPCVHQDIHVRTWHSDAADIDTAPVPDAVIETFACDLPENVLH
                     IIRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCE
                     AVRFDSEALRQRLMLPEKNAPEWLLFGYRSDVWAKWLEMWQQAGSPMTLLLAGTQIID
                     SLKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVR
                     AQLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYTPETASAHRRLSDDLNGGEALSA
                     TQRLECWQILQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(4921..5199)
                     /locus_tag="ACFNML_000960"
     CDS             complement(4921..5199)
                     /locus_tag="ACFNML_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392369.1"
                     /translation="MFRNLLVRWLAVCLIPLATLAVFAANPPEDKPQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKHQTMFLFIPIVLLVVYLFHYFGAF"
     gene            5553..6494
                     /gene="miaA"
                     /locus_tag="ACFNML_000961"
     CDS             5553..6494
                     /gene="miaA"
                     /locus_tag="ACFNML_000961"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFC6392370.1"
                     /translation="MPTPKAFALLGPTAGGKTALALKIAETLPVEIISLDSALVYRDM
                     DIGTAKPSASERAFVPHHLIDIIPPTESYSAARFVEDCTRLVGEISSRGRFALIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIGRALEVYYLTGKPMSTHLGSLTSHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPSLTADSPAIRCVGYRQAWKYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
     gene            6498..7217
                     /gene="tadA"
                     /locus_tag="ACFNML_000962"
     CDS             6498..7217
                     /gene="tadA"
                     /locus_tag="ACFNML_000962"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFC6392371.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLESLLDGTPPQEMSQAHKARLLAELKNHPPVAAFPPQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIAAAHNTCIADCNVSRHAEINALAQAGSEMQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            7222..7710
                     /locus_tag="ACFNML_000963"
     CDS             7222..7710
                     /locus_tag="ACFNML_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HFC6392372.1"
                     /translation="MTALLVILALALIAAGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYMAGIWGTKYTGAGKLAVRGALVGSIIGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTTFKIGCAVSILFILLVKYIAY
                     LF"
     gene            7776..8528
                     /gene="rlmB"
                     /locus_tag="ACFNML_000964"
     CDS             7776..8528
                     /gene="rlmB"
                     /locus_tag="ACFNML_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFC6392373.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYVQEGKNDVRTREVLEK
                     AANENIRVYFADADRLNAISKGARHQGVVGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVASGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMGGNADLYHCDLPDSAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKA"
     gene            complement(8590..9982)
                     /locus_tag="ACFNML_000965"
                     /pseudo
     CDS             complement(8590..9982)
                     /locus_tag="ACFNML_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
     gene            complement(9997..10251)
                     /locus_tag="ACFNML_000966"
     CDS             complement(9997..10251)
                     /locus_tag="ACFNML_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392374.1"
                     /translation="MLPIVAYFTAGVKSGGSGMHGAAWRRMRRVCIFVFEIRLLRFEN
                     DIPAATVRNLFAGRARMPSEIEIEAVWRIRYNPRFGFFNI"
     gene            10289..11164
                     /gene="dapA"
                     /locus_tag="ACFNML_000967"
     CDS             10289..11164
                     /gene="dapA"
                     /locus_tag="ACFNML_000967"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFC6392375.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGIYRHFKAVAEAAAIPMIIYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFVVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            11173..12369
                     /gene="bamC"
                     /locus_tag="ACFNML_000968"
     CDS             11173..12369
                     /gene="bamC"
                     /locus_tag="ACFNML_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFC6392376.1"
                     /translation="MPSEPFGRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGSLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSPAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSIRRLFDKVGLGGIYSTGERDKFIIRIEQGQNGVSDIFFTHK
                     AMKEVYGGKDKDTTVWQPSPSDSNLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVADGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(12426..13358)
                     /gene="pip"
                     /locus_tag="ACFNML_000969"
     CDS             complement(12426..13358)
                     /gene="pip"
                     /locus_tag="ACFNML_000969"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HFC6392377.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETAWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNRLIEAYHGLLFHQDEEVCLSAAKDWADWESYLICFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIQHIPTIIVQGRYDLCTPMQSAWALSKAFP
                     EAELRVVQAGHRAFDPPLVDALVQAVEDILPHLL"
BASE COUNT         3454 a         4106 c         3365 g         2741 t
ORIGIN      
        1 cacctcctac cgcaagccct tttcatcaaa tatgccgtct gaaacagaaa aatgcgtttc
       61 agacggcatt ttttccgtca atacgaatcc ggaatgtgtt tgaaatacag cataaacaga
      121 taccaaaaca gcatcgcggc ggctgcaccg agcagtaccc atgcgctgat ttcggcgact
      181 tcgcgcaggg cgcgtttttg cctgcggctg aaaaggcggt tgataatcaa cgcggcaagt
      241 gcgaaaaaga aaaaaatacg gaaaagtctg cctatcatgt cgggaagcgg tggaggtcgt
      301 ctgaaaaagt atgattcgtg tgcggcggat gagggtcaaa cgcgtccggc tgcttgtccg
      361 accgtgtcaa aaagtcatgc cgtttgcgat ataataacgc ttttcgccgc atttgttgga
      421 acaggatatg agtcaaaatg cctcggtggg cattgtaatg ccccaaaaaa ttccgtttga
      481 gatgccgctg gttttggaaa acggtaaaac tttgccgcgt ttcgatctga tgattgaaac
      541 ctacggcgag ctgaatgctg aaaaaaacaa tgcggtttta atctgccacg cgctgtcggg
      601 caaccatcat gttgcgggca ggcattcggc ggaagataaa tatacgggct ggtgggacaa
      661 tatggtcgga cctggcaaac cgattgatac ggaacgtttt ttcgtggtcg gtttgaataa
      721 tctgggcggc tgcgacggca gcagcgggcc tttgtcgatt aatcctgaaa cgggcaggga
      781 atacggcgcg gattttccgc tggttacggt gaaggactgg gtaaaatccc aagccgcgct
      841 tgccgattat ctcggcatcg ggcaatgggc ggcggttgtc ggcggcagct tgggcggcat
      901 gcaggctttg cagtgggcga tttcctatcc cgaacgcgtg cgccacgcct tggtgattgc
      961 gtccgcgccg aaactgtcca cgcaaaatat cgcgttcaac gatgtggcgc gtcaggcgat
     1021 tttgaccgat cccgatttca acgaaggaca ttaccgcagc cgcaacaccg ttcccgctcg
     1081 gggcttgcgg attgcccgca tgatggggca catcacctat cttgccgaag acggtttggg
     1141 caaaaaattc ggacgcgatt tgcgttccaa cggctatcaa tacggctatg gcgttgaatt
     1201 tgaagtagaa tcctatctgc gctatcaagg cgacaaattc gtcgggcggt ttgatgccaa
     1261 cacctatttg ctgatgacca aggctttgga ctatttcgat ccggcggcgg atttcggcaa
     1321 cagcctgacc cgcgccgtgc aggatgttca ggcaaaattc tttgtcgcca gcttcagcac
     1381 cgattggcgt ttcgcgcccg aacgttcgca cgaactggtc aaggccctga ttgccgccca
     1441 aaaatccgtg cagtatatcg aagtcaaatc cgcacacggg cacgatgcct ttttaatgga
     1501 agacgaagcc tatatgcgtg cggtcgccgc ctatatgaac aacgtttata aggaatgtca
     1561 gcaatgaacc tgcgcgatga tttgcaactg atttacgacc ggatacccga aggcagccgc
     1621 gtcttggact tgggctgcgg cgacggcgaa ctgcttgccg cattggtcga acacaaaaaa
     1681 tgcagcggct acggcatcga aatcgacacc gacagcgtca tcgccgcgat gtcgcgcggc
     1741 gtaaatgtta tccaagccga tttggaagaa ggtttaaccg cattcaacga tcaaagtttt
     1801 gatgtgattg tgttgagcca aaccatccaa gcgatgcaga ataccgaaaa aatcctgcgc
     1861 tgcctgatgc gcgttgccaa acaggcgatt gtcagcttcc cgaatttcgg ctactggcgc
     1921 aaccgcgtcc aaatcgcact cggcggacat atgccggttt ccgaacgtat gccctaccat
     1981 tggtacgaca cgcccaatat ccattggtgc acgctcaaag attttgattt gttgtgcgcc
     2041 aaaaacaaca tccgcgtcct cgaacgcgcc gtgatgacga gcaacagaca ggtcaaacat
     2101 ttccccaacc ttttgggcag cctggcgttt taccgcgtcg ggtaagacag gttgagaacc
     2161 ttgtcggcaa taacaggttc ttcggttcta ttaacaacag gccgtctgaa aaatgaaacg
     2221 gtaggttttc agacggcctc tttaatactg gaaacaatag aatgaaaaac aaaacctcat
     2281 cacttctctt atggcttacc gcaatcatgc tgaccgcgtg ttctccgaac aaaaacgata
     2341 aaaccaaaga agtcggtgca tctgctgctt cgtcctccgc gtcatcagct tcttcccaat
     2401 ccgatttgca accggccgca tccgcccctg ataacgtcaa gcaggcagaa agcgcgccgc
     2461 cgtcaaattg caccgacctg caccccgcca ccggcattga cgatctcatg cagcaaatcg
     2521 ccgaacacat cgactcggac tgtctgtttg ccctttccca tcacgaactg gaaacccgtt
     2581 tcggcttacc cggcggcggc tatgacaaca tacagcggct gctgtttccc gacatccgcc
     2641 ctgaagatcc cgactaccat cagaaaatca tactggcaat tgaagacttg cgttacggaa
     2701 agcgcacgat cagccggcag gcacaagatg ccttgatgga acaggaacgc cgcctccgag
     2761 aagcgacgct gttgctgata cagggcagtc aagaaacccg cggacaaggc gaggagccga
     2821 aacgcacgcg ttattttgaa gtttcggcaa cccctgccta ttcgagccgg cacaacaacg
     2881 gacttggcgg caatttccaa tacatcggcc aattgcccgg ctatctgaaa atacacggag
     2941 aaatgcttga aaaccaatca ctcttccggc tgtccaaccg tgaacgcaat cccgacaaac
     3001 cgtttttaga catccatttt gacgaaaatg gcaaaatcac gcgtattgtc gtttacgaaa
     3061 aaaacatcta cttcaatcca aacttggggc gaagataaaa aaatgccgtc tgaaacgttt
     3121 tcagacggca tttttcaatg caacaaatca agcgcgtttg cggaattcgc cggtgcgggt
     3181 atcgatttcg actttgtcgc cgttttcgat gtaagacatc acttggattt cggtgccgcc
     3241 gaccaggcgc gcggttttca tcactttgcc ggaagtgtcg cctttgacgg caggctcggt
     3301 gtactcgact tcgcgcacga tgatggtggg cagttcgaca gaaatggggt tgccttcgta
     3361 gaaggtaact tcgcattggt cttccatacc gtcaacgatg aatttcaacg cgtcgccgat
     3421 gttgtcggct tcgatttcgt attggttgaa ttcttcatcc ataaagacgt acatcgggtc
     3481 ggcaaagtag ctgtacgtac agtttttgcg ggacaggatg accacgtcga atttgtcgtc
     3541 ggctttgtaa atggtttcgg aagccgcgcc ggtcagcagg tttttcagtt tcatgctgac
     3601 tttggcggaa gagcggccgc ctttgatgta ttcggttttt tgaacgacca taggatcgtt
     3661 gccgaccata aatacattgc cggcgcgcag ttcttgtgct gttttcattg tgattttcca
     3721 atcaaatcgg ttgtcgtaaa acgcgctatt ctagcgtatt ttttgatgct ttgaaacaaa
     3781 ggcggcgagt ttttcggatg cggaaggctg cccgaaaaga taacggctcc aatcctccgc
     3841 gccttgccgc cagccgtttt gatgttgttg caggatttgc caacattcga ggcgttgtgt
     3901 tgcggataaa gcctctccgc cgttgaggtc gtctgaaagg cggcggtgtg ccgatgcggt
     3961 ttcgggcgtg tagaaaccgt gtgccttatc ccaaaaggcg tggagtttgt cgagatggac
     4021 attctcgtct tgcggataga tgtgccaaaa gaagggtttt ccggcaagct gggcgcgcac
     4081 gaaactgtct tcgccgcgga tgacggcgca gtcggcaagg tgcagcagtt tgtcgaagtc
     4141 ctgttgcggc acgaaaggga ttttgacgag gcggacggat gccgtctgaa aaacatcgcc
     4201 gtcgttttgc agggcatctt gcggaataac gccgctttgt ttgaggctgt cgatgatttg
     4261 cgtccccgcc agcaacagtg tcatcgggct gcctgcctgt tgccacattt ccagccactt
     4321 tgcccaaaca tcgctccgat agccgaaaag cagccattcg ggggcgtttt tttcgggaag
     4381 catcagccgc tgacgcaggg cttcgctatc gaaacggacg gcttcgcagt aatcgcgttc
     4441 gcgtatcagt ccgccgcttt tttcgctgaa acccataaac caaaaatatt tttgaacacc
     4501 ttcctgcggc gaaggcatca ggtgcagcct ttcattgctt tcctccgcgc tcaaatattc
     4561 ccaattcagc caaagcggct tgtgtcggcg gataatgtgc agcacatttt cgggcaggtc
     4621 gcaggcaaaa gtttcgatga cggcatcggg aacaggcgcg gtatcaatat ctgccgcatc
     4681 ggaatgccaa gtgcggacat gaatatcctg atgaacgcag ggaacatcgg gcaaatcagg
     4741 gcaaagcgcg cgcaaggcgg acacatcgtc cgtccacaaa tgcacctgcc aaccgagttc
     4801 gcggtgcaaa acacgggcaa gccgccacga aacgccgatg tcgccgaaat tgtcgatgac
     4861 cttgcaaaaa atccaacaga caggaaaagc gtatgtattc ataaccatct agaaaacaag
     4921 ctaaaatgcg ccaaaataat ggaacaaata cacaaccagc aaaacaatcg gaataaacag
     4981 aaacatagtt tgatgtttca aatcaaactc ttgtttcaaa tgatgcttga tggtgtcgaa
     5041 aaggacgaat ttgaacagaa acgtcgcttc gcaggcaagg atgatgccgt taatcagatg
     5101 ctggggtttg tcttcgggcg gattggcggc gaaaacggca agcgtcgcca agggtatcag
     5161 gcagacggca agccagcgga caagcaggtt tcggaacatc gcaaggcttt caaacaaaaa
     5221 aagcaaaacg ccattatcgc cccaagcacg cgccttgtct aacggatgcg ccgacgatgc
     5281 cgtctgaagc cttgccgcag gaaaacaccg caccgccgaa cctttaaata cgcccgagac
     5341 gggcgttccc cgaaaaatcg gacgctgccg ccgatgcctg aggacaaggc tgcatcgggc
     5401 gcgggcatat tgaaaacccg gcgcgtcggt ttgaaatttt ataaaggcat acggtaaaca
     5461 cgcccccttg ccgtcggcgt gcttccgcaa accaaaatat aacgtcggca cgctttccgc
     5521 tataatccgt atttttccaa acctgaaacg ttatgccgac cccgaaagcc tttgccctgc
     5581 tcgggccgac cgccggaggc aaaaccgcgc tcgccctcaa aattgccgaa accctgcctg
     5641 tcgaaatcat cagcctcgat tccgcgctgg tttaccgcga tatggacatc ggcacggcga
     5701 aaccgtccgc ctccgaacgc gcctttgtcc cgcaccacct tatcgacatc atcccgccta
     5761 ccgaatcata cagcgccgcc cgctttgtcg aagactgcac gcgcttggtc ggggaaattt
     5821 cctcacgcgg caggtttgcc cttattgtcg gcggcacgat gatgtatttc cgcgccctga
     5881 cccaaggttt gaacgatttg cccgaagccg acgcctgcct gcgtgccgac ttggacgaac
     5941 aaaaacagat gtacggcttg gatttcctct accgcaccct gcaaaaagtc gaccctgaaa
     6001 ccgcatgccg tctgaaaccg aacgacagcc agcgtatcgg acgcgctttg gaagtttatt
     6061 atttaaccgg caaaccgatg agcacacatc tcggcagcct gacctcccat acgctccctt
     6121 ttgatttgca taccgccgcc ctgattcccg aaaaccgcgc ccgcctgcat gaaaacatcg
     6181 ccctgcgttt ccacctgatg cttgaacagg gctttatcgg cgaagtggaa aacctgcgcc
     6241 gccgctatcc cagcctgacc gccgactccc ccgccatccg ctgcgtcggc taccgtcagg
     6301 catggaaata tctggatgga aaaaccgatt tcccggcatt tgtcgagaaa ggcattgccg
     6361 ccacccgcca acttgccaaa cgccaactga cttggttgcg caaaacacct ttagactgcg
     6421 ttgccgaccc gttttcagac ggcacttcat gcacacgcct gattgaggcg gcaaaacggt
     6481 ttttcggcgc ataaaccatg ctgaccacac cgccgctcgc ccccaaaacc gtcgccgccc
     6541 tgcacaggct cggtatccgt acgctcgaag aactgcggca aaacggttcg gtcaaagcct
     6601 ttctgctgct caaagcatcg ggtttgaccc tcacgaaaag cacgctgtgg caactcgagt
     6661 cgctgctgga cggcacgccg ccgcaagaaa tgtcccaagc gcataaagct cgcctgcttg
     6721 ccgaattgaa aaaccatccg cccgtcgccg cctttccgcc gcaggaagaa atggaacact
     6781 tcatgcgcga agcactgcgc caagcagaac aatccgccgc cgacggcgaa atacccgtcg
     6841 gggcagtcat cgtttcagac ggcaaaatca tcgcggcggc acacaacacc tgcattgccg
     6901 actgcaatgt cagccgccac gccgaaatca acgccctggc acaggcaggc agcgaaatgc
     6961 aaaactaccg ccttgacgga tgcgacatct acatcaccct cgaaccctgc gccatgtgcg
     7021 cgtccgcact gatacaggca cgaatcaggc gcgtgatcta cggtgcggca gaacccaaaa
     7081 ccggcgcggc aggaagcatc gtcaacctgt ttgccgacaa acgcctcaat acgcacaccg
     7141 ccatacgggg cggaatcctg caagaagaat gccgcgccgt attaagccgt tttttccaaa
     7201 acaaaagaaa aggttgaacc tatgaccgcc ttgctcgtca tcctcgccct cgccctgata
     7261 gccgccggta cggcgggcat cgtttacccc gccctgcccg gattggcatt gatgtttgcc
     7321 ggaacctggc tgctcgccta ctccggcggc taccaaatct acggcgcggg cgttttgtgg
     7381 acggtcggac tcatcagcct tgccggcata ctggcggact atatggcagg catatggggg
     7441 acaaaatata ccggagcggg caagctcgcc gttcgcggcg cattggtcgg cagcatcatc
     7501 ggcatatttt tctcccttcc cggactaata ctcggtccct ttatcggcgc ggcggcaggc
     7561 gaactgatcg atcggcgcaa tatgcttcag gcaggtaaag cgggcttggg tacgctgttg
     7621 gggcttatcg tcggtacgac attcaaaatc ggctgcgccg tatccatctt gtttatcctg
     7681 ttggtgaaat acatcgccta cctgttttaa ccccttcaga cggcataccg gcaaattgct
     7741 ataatgccgt ctgaaccctt ttcacaaagc accatatggc aaaccaaaga cctatctacg
     7801 gcttccatgc cgtcaacgcc cgattgtggc aaaaccccaa atccatcgtc gaactctatg
     7861 tccaagaagg caaaaacgat gtacgcacgc gcgaagtgtt ggaaaaggcg gcaaacgaaa
     7921 acatccgcgt atattttgcc gatgccgacc gcctcaacgc catcagcaaa ggtgcgcgcc
     7981 atcagggcgt ggtcggattt atcgatgcct ccaaaaacca cgtccacctc gaagacgtat
     8041 tggaaaactt aagcgaaccg ccgctgctgc tgatactcga cggcatcacc gatccgcaca
     8101 acctcggcgc gtgcctgcgt accgccgacg caatgggcgt acatgccgtc atcgcaccga
     8161 aagacaaaag cgcggggctg aacgccaccg tcagcaaagt cgccagcggc gcggcggaaa
     8221 ccgtccccta catcaccgta accaacctcg cccgcaccct gcgcgagctg aaagaatacg
     8281 gcatttggat catcggcacc gatatgggcg gcaacgccga cctttaccat tgcgatctgc
     8341 ccgacagcgc ggcatgggtg atgggcaacg aaggcgacgg tatgcgccgc ctgacacgcg
     8401 aacattgcga catgctggtg tccataccca tgttcggcac ggtcgaaagc atgaacgtct
     8461 ccgtgtctgc cggaatggta ttaagcgaaa cccgccggca gcgcgtatta aaaaacgaaa
     8521 aagcgtaagc ctcaaaaaca atgccgtctg aaacagggat tctgtttcag acggcattcg
     8581 ttttcaaaat cagtgttcca agtggtcggt atcaaacttg accgccgcct cggttttatc
     8641 ttcgggcaag accaaattca gcaagactgc cgtaatgccg ccggcggaaa tagagttttg
     8701 gaacaagaca ggcaggtttt taaacacttc cggctcaaac gccacgccca agcccaagcc
     8761 gaccgaagtc gccgcaatca ccgcctcgcg cctgcggatg ccgtggctga ccaaaatccg
     8821 cacacccgca atcgcaatca agccgaacat caaaaccatc gcgccgccta aaaccggact
     8881 cggaatcgtc gtaaacgcgc gtccgacaac gggggaacag acccaacagc accaaaatca
     8941 cggcaatata tttgcccaca tggcgcgaag ccacgccggt catctgaatc acgccgttgt
     9001 tttgcgcaaa cgttgtcagc ggcagcgaac ccaaagccgt cgcaatcacc gacaccaagc
     9061 cgtctgccaa cacgccgccg cgcaggcgtt tggtgtattc ctcgccttca atcggctgat
     9121 cggacaccat tgccgttgcg gtcaaatcgc cgaccgcctc aaacacgctc aacaggaaaa
     9181 tcgcacccgc cacaataaat gcgtgccagt caaaagcaaa accatattta aacggtacgg
     9241 gcagcgtaac cagcggcagg ttttgcagtg ccgaaaaatc caccttgccc aaaaacagcg
     9301 cgacgatata gccggcaatc agaccgaccg caatgccgct catccgcagc agcgggtttt
     9361 tcatgcagtt gaacaccagc acaatcagca gcaccagcga tgccagcccc aagttttcca
     9421 tcgagccgaa cgtgccgtcc gcctttgcgc cgaagccgcc gccgaaatcg gtaataccga
     9481 cgtgtaccaa actcaagccg atcagcatca ccaccacacc gctgaccgtc ggcgtaatca
     9541 cttttttcaa atacggcaga agccacgccg agaaacacac caaaaacgcg ccgacaaacg
     9601 atacgcccaa gagcgtcgaa atcatcgcat ccttagtcaa accgccctct ttcatccccg
     9661 cgcccagcgc aatcatgacg gtaacgaacg agaaattcac cgactggata gacagcatcc
     9721 ccgaaccgac cggcccgaag cggttgacct gcaaataagt gccgacaccc gacgcaacca
     9781 tcgccatcga cacgagatac gccgtcatct ccaccggcag ttccagcgcg ccgcccacaa
     9841 tcagcgcggg cgtaatcatc ggcacgaaaa tcgccaaaag atgggtaacc gcgctcaaca
     9901 gcgcattacc gaacggcggc ctatcctcca aaccgtacac caaatcaggc gcatccgccc
     9961 cttttcccat atgaccgctc ataatgtttc cttgcttcaa atgttgaaaa aaccaaagcg
    10021 cggattgtag cgaatccgcc ataccgcttc aatttcgatt tcggacggca tacgcgcccg
    10081 tccggcaaac agattccgaa ccgtcgccgc cggaatatcg ttttcaaaac gcaataacct
    10141 gatttcaaac acaaaaatac aaacccgacg catccgccgc caagccgccc cgtgcatacc
    10201 ggacccgccg gatttcacac ctgcggtaaa gtaggctaca atcggaagca ttaccgcccc
    10261 ctacaccaca actgataaaa ggttctgtat gttacaaggt agcctggttg ccctgattac
    10321 cccgatgaat caagacggca gcatccatta cgaacaactc cgcgacttaa tcgactggca
    10381 cattgaaaac ggcacggacg gcatcgtcgc cgtcggcacg acaggcgaat ccgccaccct
    10441 ctccgtcgaa gaacacaccg ccgttatcga agccgtcgtc aaacacgttg ccaaacgcgt
    10501 ccccgtcatc gccggcacag gggcgaacaa caccgtcgaa gccatcgccc tttcccaagc
    10561 cgccgaaaaa gccggcgcgg actacacctt atccgtcgtc ccctattaca ataagccctc
    10621 ccaagaaggc atttaccgcc atttcaaagc cgttgccgaa gccgccgcga ttccgatgat
    10681 tatctacaac gtgcccggcc gtaccgtcgt cagcatgaac aacgagacca tcctgcgcct
    10741 tgccgaaatc cccaacatcg tcggcgtgaa agaagccagc ggcaacatcg gcagcaacat
    10801 cgaactcatc aaccgcgcgc ccgaaggctt cgtcgtcctg tccggcgacg accacaccgc
    10861 cctaccgttc atgctctgcg gcggacacgg cgttattacc gtagccgcca acgccgcgcc
    10921 caagctcttt gccgatatgt gccgcgccgc gcttcaaggc gatatcgccc ttgcacgcga
    10981 actcaacgac cggctgattc cgatttacga caccatgttc tgcgaaccca gccccgccgc
    11041 accgaaatgg gcggtatccg ccctgggcag atgcgaaccg cacgtccgcc tgccgctcgt
    11101 tccgctgacg gaaggcggac aggcaaaagt ccgcgccgcc ctgaaagcct caggacaact
    11161 ctaaatataa ctatgccgtc tgaaccgttc ggacggcata acgcaacaaa cactttaata
    11221 tccatcacac aggatgacac gatgacccat atcaaacccg tcattgccgc gctcgcactc
    11281 atcgggcttg ccgcctgctc cggcagcaaa accgaacagc ccaagctcga ctaccaaagc
    11341 cggtcgcacc gcctgatcaa acttgaagtc ccacccgatt tgaacaaccc cgaccaaggc
    11401 agcctctacc gcctgcctgc cggttcgggc gccgtccgcg ccagcgattt ggaaaaacgc
    11461 cgcacacccg ccgtccaaca gcctgccgat gccgaagtat tgaaaagcgt caaaggtgtc
    11521 cgcctcgagc gcgacggcag ccaacgttgg cttgtcgttg acggcaaatc ccctgccgaa
    11581 atctggccgc ttctgaaagc cttttggcag gaaaacggct tcgacatcaa atccgaagaa
    11641 ccggccatcg ggcaaatgga aaccgaatgg gcggaaaacc gcgccaaaat cccgcaggac
    11701 agcatccgcc gcctcttcga caaagtcggc ttgggcggca tctactccac cggcgaacgc
    11761 gacaagttca tcatccgcat cgaacagggt caaaacggcg tttccgacat cttcttcacc
    11821 cacaaagcca tgaaagaagt gtacggcggc aaagacaaag acacgaccgt atggcagccc
    11881 tccccgtccg attccaatct tgaagccgct ttcctgacgc gctttatgca atatttgggc
    11941 gttgacggac agcaggcgga aaacgcatcg gcaaaaaacc cgaccctgcc cgccgcaaac
    12001 gaaatggcgc gtatcgaagg caaaagtctg attgtctttg gcgactacgg cagaaactgg
    12061 cggcgcaccg cgctcgccct cgaccgcatc gggctgaccg tcgtcggtca aaacaccgaa
    12121 cgccacgcct tcctggttca aaaagccccg aacgaaagca acgcagttac cgaacaaaaa
    12181 ccggggctgt tcaaacgcct gctgggcaaa ggcaaagcgg agaaacctgc cgaacagccg
    12241 gaactgattg tctatgcaga acccgttgcc gacggctcgc gcatcgtcct gctcaacaaa
    12301 gacggcagcg catatgccgg caaagacgca tccgcactgt tgggcaaact ccattccgaa
    12361 ctgcgttaag tcgttcaaaa caaaagcagc ttccaaacgg aagctgcttt tttgtgcgga
    12421 actttttaca acaaatgggg caaaatatcc tcaactgcct gaaccaacgc atccaccaaa
    12481 ggcggatcga acgcacgatg ccctgcctga accaccctca attccgcttc gggaaaggct
    12541 ttcgacagcg cccacgcact ctgcatcggc gtacacaaat cataccgccc ctgcacgata
    12601 atggtcggga tatgctgtat tttgccgata ttgttcaaaa tcgccctatc gccctgcaac
    12661 cagccgccgt tgacaaaata atggttttcc aaacgcgcga ttgccagcga ggcataagca
    12721 tcttcatcca cttcctccgg ctcgaaacag atcagatagc tttcccaatc cgcccaatcc
    12781 ttcgcggcag acaggcacac ttcttcatct tgatgaaaca gcaacccgtg atacgcctca
    12841 atcagccggt tccgccgatt ttcagcaatc ggcgcgacaa atttttgcca ttgttccgga
    12901 taaatccggc tcacaccgcc cgcctcgttc agccacgccg tttcagacgg cctgcacaaa
    12961 aatatcccgc gcaacaccaa tccctttacc cgttccggat gggtttgggc ataagccagc
    13021 gacaaagtgc tgccccacga accgccgaac accagccatt tcccgatacc cagcatttca
    13081 cggacttttt caatatccgc caccaaatcc caagtcgtat tgtcttccgc acaagcatac
    13141 gggcgcgaac gtccgcaacc gcgctggtcg atgatgacga tgcggaacac atcgggatta
    13201 aaaaaacccc gacattcagg cgaagccccc gcgcccggcc cgccatgtaa aaaaataacc
    13261 ggcacaccgt cgggattgcc cgattcctcc caataaattt gatgaatttc agacacctgc
    13321 aagtatccgc tatgaaaagg ctgttttatc tcatacatca tcttctccca tattgcttct
    13381 gataatccga acattcccac acgcaacatc acgcacgaga cacaagccgc cccgaaaggc
    13441 aaatgccgaa aaatcgcgat atagtgaatt aacaaaaatc aggacaaggc ggcgaagcct
    13501 cagacagtac agatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt
    13561 tctctttgag ctaaagcgag gcaacgccgt actggttttt gttcatccac tataacagca
    13621 accctgtcgc cgtcattccc gcaaaagcgg gaatccagtc cgttca
//
LOCUS       DAXHTR010000050        13583 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.050, whole genome shotgun sequence.
ACCESSION   DAXHTR010000050 DAXHTR010000000
VERSION     DAXHTR010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13583
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.050"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            234..446
                     /locus_tag="ACFNML_000970"
     CDS             234..446
                     /locus_tag="ACFNML_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HFC6392378.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            594..2177
                     /locus_tag="ACFNML_000971"
     CDS             594..2177
                     /locus_tag="ACFNML_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HFC6392379.1"
                     /translation="MSSKKKKAAFARAATENGVPDVSNIKNSYDDLMYESGAFSQTAI
                     NNLEARARLMGLQPAPAANAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIERMGLENVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDTVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLRDIMQFSSRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLGGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTNLH
                     VSFVSWMAEHTRERILALAGDDYIAKEFYSDILSDRQFRRSLLCREEVGDTVRRDESV
                     AVEVIESLNFRPARGETINFDENDILLSGIRDVMKTGEAFKTEDVAENLDRRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQALIETVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            2259..3356
                     /locus_tag="ACFNML_000972"
     CDS             2259..3356
                     /locus_tag="ACFNML_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFC6392380.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALTQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSDGSHTRQRMAMDVFAANTIRAVRMQSPRVG
                     VVAAAKAGGQVLFGFNATDGAFEVVPQVFSWQLSFTDNIGVIQQNFLDLMAECRVEHV
                     YLLHNPLGEQENIPSYAEALKREGHNPFFSA"
     gene            3470..4585
                     /locus_tag="ACFNML_000973"
     CDS             3470..4585
                     /locus_tag="ACFNML_000973"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HFC6392381.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     ACGQSLLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            4710..5165
                     /locus_tag="ACFNML_000974"
     CDS             4710..5165
                     /locus_tag="ACFNML_000974"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HFC6392382.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RSEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            5236..5712
                     /locus_tag="ACFNML_000975"
     CDS             5236..5712
                     /locus_tag="ACFNML_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HFC6392383.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            5700..6626
                     /locus_tag="ACFNML_000976"
     CDS             5700..6626
                     /locus_tag="ACFNML_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFC6392384.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMEQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVDPRQFINPDYAQQALYKDQIGICDVLAASKTD
                     LCTPEQLVEFHDKAAKLFPPKAKVVEVRNAQLDIQWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            6691..7245
                     /locus_tag="ACFNML_000977"
     CDS             6691..7245
                     /locus_tag="ACFNML_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6392385.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVE"
     gene            complement(7484..8443)
                     /gene="ttcA"
                     /locus_tag="ACFNML_000978"
     CDS             complement(7484..8443)
                     /gene="ttcA"
                     /locus_tag="ACFNML_000978"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFC6392386.1"
                     /translation="MSKKIKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRRLQASAPIDFQLVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATLFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFVGLERGQSLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIEPQKAERKVINILANKPKTCGA"
     gene            complement(8536..9399)
                     /locus_tag="ACFNML_000979"
     CDS             complement(8536..9399)
                     /locus_tag="ACFNML_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HFC6392387.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKVVFEILEDYAVGAEL
                     EASAEPLAAQEPSLVFTAECVSDGICSVVLPHRGILHIAPETALPPYDAAAENAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAVEAGTLLTADGEEAGIVLDSVQDSENFTALTVIKFSAAQKELTVPNGGIFKA
                     VHLFFKTENAE"
     gene            complement(9434..9796)
                     /locus_tag="ACFNML_000980"
     CDS             complement(9434..9796)
                     /locus_tag="ACFNML_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6392388.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAVKRDAATA"
     gene            9871..10350
                     /gene="ybaK"
                     /locus_tag="ACFNML_000981"
     CDS             9871..10350
                     /gene="ybaK"
                     /locus_tag="ACFNML_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HFC6392389.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGNGLIVLMHGDKQISTRNLARHLGAKRIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLETIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            10372..11094
                     /locus_tag="ACFNML_000982"
     CDS             10372..11094
                     /locus_tag="ACFNML_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HFC6392390.1"
                     /translation="MDKDLYAVLGVSPQAGADEIKRAYRKLAMKYHPDRNPGNPQAEE
                     KFKEIQRAYDTLSDLSKRMQYDASFRRHEERGRQEEAFRREQARREQFYREQMRREQA
                     FRQAFERQASRSCHTYEPSGGGSGRNYVLAAYILFGLGAIMLFMPIVGVIFAYMPIVG
                     VILAYMKRNSLDSIVYAAHTEYLIKTFWRTFWLYILGALTALLGIGVLIIIATNVWYF
                     YRIIAGFIRFNGGRAVAPEKWI"
     gene            11096..11971
                     /locus_tag="ACFNML_000983"
     CDS             11096..11971
                     /locus_tag="ACFNML_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HFC6392391.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWWLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGRLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGSLLVAFVLAGVLMFACLFAKSVRWRVESVVGGIFLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(12043..13440)
                     /gene="aspA"
                     /locus_tag="ACFNML_000984"
     CDS             complement(12043..13440)
                     /gene="aspA"
                     /locus_tag="ACFNML_000984"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFC6392392.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGKLGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEVLGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLAVLKNAFAAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAVNLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
BASE COUNT         3246 a         3307 c         3728 g         3302 t
ORIGIN      
        1 aacgacgtac tggtttttgt taatccacta taaatgcgcc gattgccaag gcgatgatgc
       61 tcaaccaggg tttttgcgaa gcgttcattg ttgtcgtcct gatgggaatt gccgccatta
      121 tgcgcaagcg tccgtctttt tccaaaccgc cgcaccgttt ccgcgtttgc ccaagttcac
      181 gccccgactt acaataacgc ccgtcagatt gcagttccaa cgaaaggaaa atgatggaaa
      241 cccttatcct cgacatcggc ggcatgagct gcggcggctg cgtcaaaagc gttacccgga
      301 tattggaagg cgtaaaaggc gtggcaagcg tcgaagtcag ccttgaaaac aaaagtgcga
      361 ccgtcggata cgaccccgcg caaaccgatg caggggcgtt gattgaagcc gttgaagacg
      421 gcggctatga tgccgcgttg aaataaagcg gcaaaaatgc cgtctgaagc cttcgcgcct
      481 cctagatgcc gtctgaagta cgctccgcgc cttcagatgg catttgattt agttgaacgg
      541 gtaggatgaa ttggttaaac ttgcgggcat attgtcttta gagtgaacaa aatatgtcga
      601 gcaagaagaa aaaagccgct tttgcccgtg ctgcaacgga gaacggtgtg ccggacgtgt
      661 ccaacatcaa aaattcatat gatgatttga tgtacgaatc cggcgcattc tctcaaaccg
      721 ccatcaataa tttggaggcg cgcgcccgcc tgatgggctt gcagcccgcg cccgccgcca
      781 atgccaaggt cttggagctg ggctgttcga tgggcggcaa catcatcacg caggcgcttt
      841 attacccgga tgcggaattt gtcggtatcg acttgtccgg caggcaggtt gcgcagggca
      901 acgccatcat tgaaagaatg ggcttggaaa atgtgcgtct ggaagaaaaa gatattttga
      961 ccatcgatga gtcattcggg aagttcgact atatcatcgt ccacggtatt tggtcgtggg
     1021 ttcccgatac ggttaaagac aaaatctttt cgatttgccg gaacaacctg acggaacacg
     1081 gcattgccta tatttcgtac aatgtctacc ccggctggaa acggcaggag cagttgcgcg
     1141 atattatgca gttttccagc agggatgtgc ttgaagaacc cttggaagcg cgaacgcgga
     1201 aaggcttgga cgcgctcaag gcactggcgg aaattttgga aaacgacaag ggcttgggcg
     1261 gcggcggcaa acttccggcg attcaaaaaa tattgaatca taatttttat tacatcgcac
     1321 acgaatatat ggaggcgttt aacgacccga tttacgtcaa cggcttcatc gaatgggcta
     1381 accgccacag gctcgcatat atcggggata ccaatttgca cgtgtctttt gtttcttgga
     1441 tggcggagca tacgcgggag cggattctgg cattggcagg ggacgattat attgccaaag
     1501 aattttacag tgatatttta tccgaccgcc aattccgccg ttcgctttta tgccgtgagg
     1561 aagtcgggga tactgtcagg cgtgatgagt cggttgccgt cgaagtgata gaaagtttga
     1621 atttccgccc ggcaagaggg gaaacaatca actttgatga aaacgatatc ctgctttcgg
     1681 gcatacgcga tgtgatgaaa accggagagg cgtttaaaac ggaagatgtt gcggaaaatc
     1741 tcgaccgccg atttcccggt ctggaatttg accgcatgaa aatcaattcc cagctgttat
     1801 tgcaaaccat tctcgggcgt ttttctgttt catcagacaa tgcgggcaaa ccattttttg
     1861 aagaccataa aacctatgtg ccggcgcgtt ttacagatta tgccgccgcc tttgtggaac
     1921 atggcgcgga agcgtttgtc cggcctgcca accgttacaa cgaaagcacc ccctcattcg
     1981 gatacgggca tttgtacatt atgcggcaat tgtcgcggcc gacgagcaaa caggcattga
     2041 ttgaaacggt tgccgaaaac ttgaacatcg tcagcaccac gcccgacggt ttgacattcc
     2101 atccgcctgc cgaagtgtat gtggaagaaa tattggcaga cttggcagac aggcattttc
     2161 tcgtttcggc ggattgacgg gcaagtcccg gcggcaatcc atccgatata ttttcagaca
     2221 ggaaagacac acactacgaa aagcggtgca cgacacatat gaaccagaca tttaccttgc
     2281 ccgatacgcg cccgtatccc caaaatccga ttaaaaacca cctgctgctc aatgcctacc
     2341 agttggcgca taattcttcc caggcttcgc gcaaactctc gtccggccaa cttcaaaccg
     2401 aaatcagggg gatgcttgag caaaaccact atatcaacct ttccctcgcg ctgacgatgt
     2461 cgcccgatgc cggaacttat gccgcgctgc tttccagtgt gaacgcagta ctcgattgcg
     2521 agaaagaagg cgaagtgcag tggttcgccc tgccggtcgt gctggtgtcc ggctgcaaga
     2581 aagaacgggc aatcgagatg aagctgccga cggaggcatt gtttgcctgc ctgcaaaact
     2641 atccgcacct gcgcgcgttg acgcaagaga cacaatggct gccttatctt gtgcattcgt
     2701 ccgatttgag tgcggtcgcg ccggatgagt ggtggcgtgc caaacaaaat accgaagcgg
     2761 cggcgcaaca cttacgccgt ttcgccccgc gccctttgct gttgcccgaa gggcagtccg
     2821 tccacgtcgt ttacgcgctg ggtttcggca gcggcaaggt tcagacggca ttgggtcaga
     2881 accttcttca ggcaggcctg cccttaatgc aggtatggca ggaaaatctt gcatcggaag
     2941 gtgttacgct gtttgccaat ccgctttccc cggattctcc ggtacgcgcg ctttcagacg
     3001 gcagccacac gcgccagcgt atggcgatgg atgtttttgc ggcaaacacg atacgcgccg
     3061 tccgtatgca gagtccgcgc gtcggcgtgg tcgctgcggc aaaggcgggc ggacaggttt
     3121 tattcgggtt taatgcgacc gacggcgcgt tcgaagtcgt gccgcaggtg ttttcgtggc
     3181 agctttcctt taccgacaat attggcgtta ttcagcaaaa ttttttggat ttaatggcag
     3241 aatgccgtgt agaacacgtt tacctgttgc acaatccttt gggcgaacag gaaaacatcc
     3301 cgagctatgc ggaagcgttg aaacgggaag ggcacaatcc gtttttcagt gcatagtgat
     3361 ttgtagtagt gataggcttt ctcatttcta taataaatca aaataaagag cacaacactt
     3421 tttcattctg tgttgtgcct tgagtgaaac gaaaggatga attataaata tgaagattgt
     3481 aattgcaccg gattcgttta aggaaagctt gacagctcaa caagtagctg aagcaataaa
     3541 aagaggcttc caacaatcga tagcagatgt ggaatgtctc ctctgtcctg tcggtgatgg
     3601 gggagaaggc actgtagatg ctatccgaca ttctcttgac ctagaagaaa aatgtctcca
     3661 agtgacagga ccttttggac aaaaagaagt catgcgctat tttcaaaaag aacaactagc
     3721 cctattcgaa gttgctgatt tggttggtct tggaaaaatc ccgctagaga aacgaaatcc
     3781 attacaaatc caaactcgtg gtattggaga attgattcgc cacctcatta gtcaagaaat
     3841 taaagaaatc tatattggcg ttggcggtac ggccagtaat gatggaggta ttgggattgc
     3901 tgctggttta ggttatcaat tttatgatga ggatggaaat accttgcccg cttgcggtca
     3961 atccttacta aacctagctt ctgtttcaac agaaaatcgc tatgaaattc ctgaagatgt
     4021 tcacattcgt attttagcag atgttgtgag tcccttatgt ggacaccaag gtgcgaccta
     4081 tacgtttggc aagcaaaaag ggttggattc tactatgttt gaggccgtag atcaggcaat
     4141 acaagatttt tatgaaaaag tctcccctgc aacattaaaa cttaaaggag caggagctgg
     4201 tggaggcatc gctggtggtt tgtgcgcctt tgctcaggca agtatcgtat ctggaattga
     4261 cacctgcctg gacttgattg actttgataa gaaagtgtca gatgttgact tggttatcat
     4321 tggtgaagga agactagatc gtcaaagttt agcagggaaa gcgcctattg gtgtagcaaa
     4381 aagaacccct gtcggagttc ctgttgtcgc tatttgtggc agtctcgttg aagatttgcc
     4441 ttccctgcca tttgaaaata tccaagctgc cttttctatt ttggagaaaa gtgaaccttt
     4501 agaagatagt ttgaaaaatg ccagtctcta tttggagcat acggcttcta atattggaca
     4561 cttattaaat atgcctaaga tttagccaaa ccatttcttc cagatggatg ttttggctgg
     4621 ttctgcctta tgcggttacg attaaccgac cgatgacgtc tgaaagggtt tccgcttcag
     4681 acggcatcgc gccggaacag ggaggggata tgaaaaaacc gaaaatcctt tttgtctgcc
     4741 tcggcaacat ctgccgttcg ccgatggcgg aatacatttt gcgccgccgc gccgccgaag
     4801 cgggcattcc ccttgaagcg gacagcgcgg ggacatcggg ctggcacgac ggcgaagata
     4861 tgcaccgcga gacggcaaag atattgaaaa aacacggtat cgatgcttca ggctttacca
     4921 gccgcaaaat ccgccaaagc gatgcggcgg cgtttgactg catcatcgcg atggacggca
     4981 agaatttgtc cgaattggaa agaaccttcg gcaggcggtc ggaaaaaata ttcaagctga
     5041 ccgacctgat acccgaaagc ggttacgacc atgtccccga tccgtggtac acgggcgatt
     5101 ttgaagaaac cttcaggctt gcggatgcgg gctgtcgggc attgttggaa aagatttcca
     5161 aataaagcaa ttgaatacaa atataaaacc gcctcctgcc gtatcggttg ttcagacggc
     5221 ataaacagag aatttatgaa aacaaatttc aaacagaaaa ttattgaaca ggcacgcagc
     5281 gaaggcttgc aggtaaccgc tttgcgcgag caggtattag atattgtctt gcagcaaagc
     5341 ggcgtgatta aagcctacaa cgtcttgtcg cagatgcagc agcaaagcga gggcgtgctt
     5401 gcaccgccta ccgcctaccg cgcccttgat ttttgggcgg agcagggcgt tttgcacaaa
     5461 gtggcggcgg tcaacggcta tattttgtgc agccacgcgc agcacgagtg cgacgaccat
     5521 tgccacgacc acgaagaagc cgaagcgcac cacagcgcgt ttattttggt ctgcaccgaa
     5581 tgtggcacgg cggacgagca aaccctcagc cacgagtggg cggcactgcg cgcaggcgtt
     5641 gccgaaagcg gctttgcgct gaaagaagaa cacgttgttt taaccgggat ttgtaaaaaa
     5701 tgtcagaaat gaaaaaaacc aaagtccacc tgatttcagg ttttctggga acaggcaaaa
     5761 ccaccgcgct caaaagtctg atggaacaaa aagaccccaa cgaaaaatgg gtcatcatcg
     5821 tcaacgagtt tggcgaaatc ggcattgacg gcgcggtatt gagcgacaac ggcatccctg
     5881 tggcagaaat cgccggcggc tgtttgtgtt gcaccgccgg cccgcaaatg ggcgtaaccg
     5941 tgcagaaaat gctgcgcgat gccaagcccg accgcctgat gattgaggca agcggactgg
     6001 cgcacgccgc cagcgtcatc gacgaactca aagccaaacc gctggacagc cttttggaaa
     6061 tcggcgcggt ctttaccgtc gtcgatccgc gccaattcat caaccccgat tacgcgcaac
     6121 aggcattgta taaagaccaa atcggcattt gcgacgtatt ggccgccagc aaaaccgatt
     6181 tatgcacccc cgaacagctg gtcgaatttc acgacaaagc ggcaaaactg ttcccgccca
     6241 aagccaaagt ggtcgaagtc cgaaacgcgc aactcgatat ccaatggctc gatattcccg
     6301 tcatcgaaaa atcacgctac cgcctcaaag ccctgccgga caacacgatg ggcttccagt
     6361 cgcaaggttt cacattcccc gccggacgcg atttcgacgg cgaaaaattg accaacttct
     6421 tcaatgattt gcccaagatg accgaaggac tcgtccgcgc caaaggcgtg tttcaagtac
     6481 tcggcacttg ggtgtggctc aactgggtgg acgggcaatg gggtgcgaac caagtctcgt
     6541 ggcggcgcga ttcgcgtttt gaattgattg ccaaatcgtt tgatgcggat ttaatcgaac
     6601 aaaagctcaa agacgcattg gaatagcgtt cacgcgcctt cagacggcat atgaaatgaa
     6661 aatgccgtct gaaggtctga ggaggacggg atgaaaccca aattcaaaac cgttttaacc
     6721 gcgctgcttt tggcggtttc cctgccgtct atggcggcaa cccatgtttt gatggaaacc
     6781 gatatgggca atatccgttt ggttttggac gaatccaaag cacccaaaac cgttgccaat
     6841 ttcgtgcgct atgcccgaaa aggcttttac gacaatacga tttttcaccg cgtcatcggc
     6901 ggcttcgtta tccaaggcgg cggattgacc gaggacttgg cacaaaaggc aagcgataag
     6961 gccgttgcca acgaatccgg caacggcttg aaaaacactg tcggcaccat cgccatggcg
     7021 cggacgacag accccgattc cgccaccagc caattcttta tcaatctggc ggacaatgat
     7081 tcactcaact acaaaaacgg acaatacggc tataccgttt tcggcagggt cgaaagcgga
     7141 atggacaccg tttccaaaat cgcccgcgtc aaaaccgcca cgcgcggctt ttatcaaaac
     7201 gtacccgtac agcccgtcaa aatccgtcgc gttgttgtcg agtaatataa cgggaaacct
     7261 tcagaaagct gaaacggaaa aagcaggtgt ttggggttga aaagcgtctg cttcgggatt
     7321 ttataatgga ttaaatttaa accagtacgg tgttgtctcg ccttgccgta ctatttgtac
     7381 tgtctgcggc ttcgtcgcct tgtcctaatt taaatttaat ccactataaa atgccgtctg
     7441 aaacgccaaa cgggcttcag acggcatttt tgacggcggg gggttacgcg ccgcaggttt
     7501 tcggcttgtt cgccagaatg ttgatgactt tgcgttcggc tttttgcggc tcgattttga
     7561 tttcgctttc gtcttcttcg ctgccgtctg aaaaacgttc gggcattttt tcgctgtcaa
     7621 acgccaaatc gccgccgtgt ttcaggcttt gaccgcgttc caatccgaca aagtcgaaga
     7681 gttcggtatc ggcaaggtgg gaagggacga cgttttgcag ggcggagaac atcgattcga
     7741 tgcggccggg gaagcgtttg tcccaatcgc gcagcatatc gccgatgact tggcgttgca
     7801 ggttgggttg cgagccgcag aggttgcacg ggatgatggg gaattgtttt aattcggcgt
     7861 atttgattaa gtctttttct tttacatacg ccagcggacg gatgacgatg tgttcgccgt
     7921 tgtcgctcac cagcttgggc ggcatggctt tgagtttgcc gccgtaaaac atatttaaaa
     7981 acaaggtggc gaggatgtcg tcgcggtggt gtcccaaggc gattttggtg cagcccaatt
     8041 cttttgcagt gcggtagagg atgccgcggc gcaggcggct gcacagcgaa caagtcgttt
     8101 tgccttcgtc taatacgcgt ttgacggtgg agtaggtgtc ttcttcaacg attttgtagg
     8161 gaacgccgat gctttcgaga taggtcggca atacttcttc ggggaagccc ggctgctttt
     8221 ggtcgagatt gacggcaact agttggaaat caatcggcgc gctggcttgg agccggcgca
     8281 ggatgtccaa gagggcatag ctgtcttttc cgccggagag gcagaccatg attttgtcgc
     8341 ccggctcgat catattgaaa tcgttaatcg cgtcgccgac ggcgtggcgc aggcgtttgc
     8401 tgagtttgtt gttttcgagt tcttgcttga tttttttgga cataacggtt cgggtttgaa
     8461 aattggaaag gcggcattgt aaccgattgg cggggcggca atgccgtctg aagggcttca
     8521 gacggcatcg gcggcttatt ctgcattttc ggttttaaag aagagatgaa ccgctttgaa
     8581 gataccgccg tttgggacgg tcagttcttt ttgtgcggcg gagaatttaa tcacggtaag
     8641 ggcggtaaag ttttccgaat cttgaacgct gtcgagtacg atgccggcct cttcgccgtc
     8701 cgccgtcagc agggttcctg cttcgacggc cgaatttccc gacaataccg ccaagccgcg
     8761 tttgacctgc ccccgatact gggcacgggc aatgatttcc tgtcccggat agcagccttt
     8821 tttgaagtgt acgccgccga tgatgtgctg gttgagcatt tgggcgacgg cggtttcttt
     8881 ggtagccgcg catatccacg ggtaaccgct gcggatttcg tgcagccgcc acgcgttttc
     8941 ggcggcggca tcataaggcg gcagggcggt ttcgggggcg atgtgcaaaa tgccccgatg
     9001 gggcaggacg acggaacaga tgccgtctga aacgcattcg gcggtaaaga cgagactggg
     9061 ttcttgcgcg gcaagcggtt cggcggatgc ttctaattcc gcgccgacgg cgtaatcttc
     9121 aaggatttca aaaacgactt tggcacgcaa cacaaacatc cgcaggcgtt tgacggttgc
     9181 ttcaagcagg tcttgcgcca taatcagcag caaatcgccg cctcggttga cgacaatcat
     9241 attggcgatg acgcggcctt tgggcgtgtt gtaagtcgca taacacgcct gcccggtctg
     9301 aagattattg atgtcgttgg aaagctgtcc gtgcaggaag gtttggcggt cttcgccact
     9361 gacgcgcacc acgccgaaaa agggcagtaa ggttttcatc atttgcctac tctgaaaata
     9421 taaggaaatc tgtttatgca gttgccgcgt ctctcttcac ggcggttatt ttgatttcga
     9481 cgcgccactc cggacgggcg agccgcgctt ccacgcaggc gcgggcgggc gttctgccgg
     9541 cggcaaccca agcgtcccac acgccgttca tttccgcata gtcgcccata tcgcgcagat
     9601 agatgaccgc atccaaaacg tgcgctttgt ccgagccgca ttccgccagc cagcggtcga
     9661 tttgggcaag cacgtcggca gtctgttcgg cagccgtttc accgttttcg ggaaccatgc
     9721 cagagaggaa aatcaagccg tttgcgccga cggcttcgga atagcggggc gttgtgccga
     9781 aatatcggat atccatatcg gtttccttcg ataaagggga tatatggtaa cattgcgctt
     9841 gaccgatttc catgttttgc atgacgaaaa atgagtaaac acacttatcc gataacacct
     9901 gccgtgcgcg ttttgcgtga aaacggcatc gaatttgaac cttttaccta tgcctatgag
     9961 gaacacggcg gcacggcgca gtttgcccga ctattcggca aagacgaaca cttggtcatt
    10021 aaaaccattg ttttgcaaga tgaaaacggg aatgggctga ttgtcttgat gcacggcgac
    10081 aaacagattt ccacgcgcaa tctggctcgg catttgggtg cgaaacgcat cgaacccgct
    10141 acgcccgcac aggcaaacaa gtggacgggc tatctggtcg gcggcacaac gccgttcggc
    10201 atccggacaa aattggatat ttacgtcgaa cagtcggtga tggatttgga aaccatctat
    10261 atcaacggcg ggaaacgcgg gttcattatc ggtatccgtc ccggagattt aaatattttg
    10321 aacccgaaaa caatacaggc ggcggtttga ccgggaagta taaggaacaa tatggacaaa
    10381 gatttgtatg ccgtattggg cgtgtcgccg caggcggggg cggacgaaat caaacgcgcc
    10441 taccgcaagc tggcaatgaa ataccatccc gaccgcaatc cgggcaatcc gcaggcggaa
    10501 gaaaagttca aagaaatcca acgggcttac gatacgcttt ccgacctgtc gaaacggatg
    10561 caatacgacg cgtccttcag acggcatgag gaacgcgggc ggcaggaaga ggcattccgc
    10621 cgcgaacagg cgcgcaggga gcagttttac cgcgaacaga tgcgccgcga acaggcgttc
    10681 agacaggcgt ttgaacggca ggcatcacgt tcgtgccata cttacgaacc gtccggcggc
    10741 ggaagcgggc gcaactatgt cctcgccgcc tacatcctgt tcggtttggg tgcaatcatg
    10801 ctgttcatgc ccatagtcgg cgtgattttc gcctatatgc ccatagtcgg cgtgattctc
    10861 gcctatatga aacggaacag tttggacagc attgtctatg ccgcacatac cgaatacctg
    10921 attaaaacct tttggcgcac attttggctt tatattttgg gtgcgctgac tgcccttttg
    10981 ggtatcggcg tgctgattat tattgcaacg aacgtctggt atttctaccg catcatcgcc
    11041 ggctttatcc gcttcaacgg cggcagggcg gttgcacccg agaaatggat atagtatggc
    11101 ttacctgtta atcagcatcg tgttcagcgt gtcggtttcc attttgctga aaatggcaag
    11161 gaagaaaaaa atcgacatcg cgcaggcggt cgccgtcaat tatgtggtcg cggtcatact
    11221 gaccctgctg gtattgaagc cggatgtcgg caatatcggc gcatttttgc cgacatggtg
    11281 gctgtttgcc gcattgggcg tgctgctgcc gtccgtattt gtcattatgg gcaaatccgt
    11341 agaatccgcc ggtatcgtca aatccgacgc ggcgcagcgt ttgtcgctgt ttttgccgat
    11401 tgttgccgcc ttgacgctgt ttggcgaaaa actcagcgaa ggcagactaa tcgggctgtg
    11461 cctcgcattt gccgcactgt tctgcctgct ttggaaacac agcggtggca aaaaatcagg
    11521 aagcgcgtgg cggcaggcgg cattgctgct gggcgtgtgg gcaggttacg gcattatcga
    11581 tatcctgttc aaacagcttg ccaaaagcgg aacggcattt gcgggcagcc tgctggttgc
    11641 atttgtgctg gcgggtgtgc tgatgtttgc ctgcctgttt gccaaatcgg tcagatggcg
    11701 tgttgagagt gtggtcggcg gcatattctt gggcggtttg aattttatga atatcgtaac
    11761 ctacatcacc gcgcaccaaa tgatgaagga taatccgacc ttggtttttg ccggtatgaa
    11821 tatcggcgtg attgttttgg gtacgctttc gggcgcattg ttctttaagg aaaaaatcaa
    11881 cacaatcaat acggcgggaa tcgtgttggc actgtgttct atcgcctgcc tgttttattg
    11941 gggggaagtc aaggtactgt tcggcatata atccggctga ttgatataac aaaatgccgt
    12001 ctgaagcagc atccctgctt cagacggcat ttgtctgcaa cgttacagat gggggttcat
    12061 caggttctcg ggagagagga tgcggttgag ttcttcttcg ctcaacagcc cgcgttccaa
    12121 gacaacctcg cgcacgcctt tgccggtttg ggcgcagatt ttgccgacca aatcgccgtt
    12181 gtggtgtccg atatacggat tcagataagt caccaaaccg atggagttga aaacgtaacg
    12241 ttcgcagatt tcgcggttga ccgtaatgcc tttgacgcat ttgtcggaca ggttgactgc
    12301 ggcattgccc aagagggaaa tggtttcaaa catacattgg gcgatgaccg gctccatcac
    12361 gtttaattgc agttgcccgg cttcggcggc gaaggtaatc gtcgtgtcgt tgccgatgac
    12421 tttgaagcag acttggttga ccacttcggg aatcacggga ttgactttgg cgggcattat
    12481 ggaagaaccg gcctgcaatt cgggcaggtt gatttctttc aagccggcac gcgggccgga
    12541 agagagcagg cgcaagtcgt tgcagatttt ggagagtttg accgccgtgc gcttcaatgc
    12601 gccgtgtacc atcacatatg cgccgcagtc ggaggtcgcc tcaatcaggt tttcggtcag
    12661 tttgcaaggc aagccgctga cttcggagag ttttttgacc accagttcgg cgtagccttt
    12721 gggcgtgttc acgcccgtgc cgattgccgt tgcgcccaaa ttgacttcca acagcagttg
    12781 gcgggtgcgg tcgaggttga ggatttcttc ttccaacaac acttggaaag attggaattc
    12841 ttggccggca gtcatcggca cggcatcttg aagctgggtg cggcccattt tcaaaacgtc
    12901 tttaaactct tcggctttgg cggcaaaggc gtttttcagg acggccagtt tgtcgagcaa
    12961 ttcgccgatg ctgtaataca cggcaaggcg gaagccggta gggtaggcat cgttggtgga
    13021 ttggctggcg ttgacatgat ccatcggatt gacgatatcg tagcggcctt tttcgtatcc
    13081 caagacttcc aatgcgaggt tggcgatgac ttcgttggtg ttcatattga ccgaagtacc
    13141 cgcaccgccc tgatacacat cggacgggaa ttggtcgagg cagcggttgt tcagcagaac
    13201 ttcgtcgcaa gccttttcaa tggcggcggc gatttcgggc ttgaccgcgc ccaacttacc
    13261 gtttgcctgt gccgtcgcct ttttcaccat caccatactg cggacaaact gcggcacgtc
    13321 agaaattttt tgtgtggaga ttttaaagtt ttcaatggcg cgcagggtgt ggatgcccca
    13381 atacacttcg gcgggaatct cgcggtcgcc caataaatcg tgttcgatac ggacagtcat
    13441 gtttttacct ttgtaagtcg gataattaat attgaaaaaa tgcgccatcg gaaagatgcc
    13501 gccgcaggac gaacactata ccggccggat gaaattgtcc atatcgtatg ccgtctgaaa
    13561 acgggaaacg ttgttttcgg gtg
//
LOCUS       DAXHTR010000051        13387 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.051, whole genome shotgun sequence.
ACCESSION   DAXHTR010000051 DAXHTR010000000
VERSION     DAXHTR010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13387
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.051"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(110..538)
                     /locus_tag="ACFNML_000985"
     CDS             complement(110..538)
                     /locus_tag="ACFNML_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family sulfur transferase"
                     /protein_id="HFC6392393.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLAYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(603..2351)
                     /locus_tag="ACFNML_000986"
     CDS             complement(603..2351)
                     /locus_tag="ACFNML_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HFC6392394.1"
                     /translation="MIHYKITPSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHTDSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKALYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            2678..4111
                     /gene="ccoN"
                     /locus_tag="ACFNML_000987"
     CDS             2678..4111
                     /gene="ccoN"
                     /locus_tag="ACFNML_000987"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045278 - plasma membrane respiratory
                     chain complex IV [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HFC6392395.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            4138..4749
                     /gene="ccoO"
                     /locus_tag="ACFNML_000988"
     CDS             4138..4749
                     /gene="ccoO"
                     /locus_tag="ACFNML_000988"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HFC6392396.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            4754..4924
                     /locus_tag="ACFNML_000989"
     CDS             4754..4924
                     /locus_tag="ACFNML_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HFC6392397.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            4948..5859
                     /gene="ccoP"
                     /locus_tag="ACFNML_000990"
                     /pseudo
     CDS             4948..5859
                     /gene="ccoP"
                     /locus_tag="ACFNML_000990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
     gene            5935..6279
                     /locus_tag="ACFNML_000991"
                     /pseudo
     CDS             5935..6279
                     /locus_tag="ACFNML_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
     gene            6481..7890
                     /locus_tag="ACFNML_000992"
     CDS             6481..7890
                     /locus_tag="ACFNML_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HFC6392398.1"
                     /translation="MDTQIKTEADNQSNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRADDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYYTMDEIHSDMMLGAAGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            7992..11201
                     /gene="recC"
                     /locus_tag="ACFNML_000993"
     CDS             7992..11201
                     /gene="recC"
                     /locus_tag="ACFNML_000993"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFC6392399.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSVLQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLESLSSDKLPERYFVFGISTMAPMYL
                     QLLHKLSEYCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPNIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFESQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQNFQTAAKQIDTAVLNSPKLPPLSYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLAAAEAFAQKQDWEAALKKAQTAYYGNKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLARLLADTLAAAEKKEEAGEA"
     gene            complement(11269..12837)
                     /gene="mtrF"
                     /locus_tag="ACFNML_000994"
     CDS             complement(11269..12837)
                     /gene="mtrF"
                     /locus_tag="ACFNML_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HFC6392400.1"
                     /translation="MSQTDTQRDGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYVVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            13171..13383
                     /locus_tag="ACFNML_000995"
     CDS             13171..13383
                     /locus_tag="ACFNML_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392401.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
BASE COUNT         3356 a         3224 c         3551 g         3256 t
ORIGIN      
        1 actgtaaaaa cggagaagga cagaggacgg cagtaaggtt tgccgtcctc tttttcaagt
       61 tgcggtggaa tgccgcttga aggtgtggtt taaacgtttc ggcttgtaat cagacgcggg
      121 cgttgtccag ccgctctctg aagttttcca aatttacgcc tgccgcttgg gcgcggcgga
      181 tgatttcgtc aaccggcata ccttctgccg cccggcggaa gccccacagg agggagcagc
      241 gcgtaccggt ccggcaatag gcaaggacgg gatattcggc ttgtccgatg agttggcgga
      301 aggtttcgac atcgtgtttt tggatgtcgc gtgcggtaac gggttggtga tggaatccag
      361 taacgcctgc ttgttccagc cactgtttga tttgggcgaa gtcgggttgc gattcttctt
      421 cgcggtcggg gcggttgcag atgacggttt tgatgcccag ttgcgcgatt tgttccgcat
      481 cggctttggt cagttgcgga gaaatataga ggtgttcgtc aagtttcaga atggccattt
      541 tgtttccttt actttgcggt tgtttttcag acggcatttt gtgtgcaatg ccgtctgaaa
      601 gtttaaccga acaaccagtt gtccaacagg ttccggtctg tgatatgtag gatggcagta
      661 tccgctgccg ctgcctgaac cgtcaagacg gtttgacgca atatgccggc acggaagaag
      721 tggatattga tttttgcatt gacgtggtat cgggcccatt gtgcggtaaa gtcggtgcag
      781 gcataaccgt ctaaagcaat gattttgtct tgcgggcaca gtgccgcaga ttccgcgctg
      841 ccgccgttga agacatgggt caggacgatg tggtcggtgt tttgtttgaa acgtgcgcca
      901 aaatcgcctg ccgacgggac ggggcagatg tgttctgcgt atccgccgcc gtgttgtcgg
      961 ggaagcggca ggaaggtcag tcccacgcct gcggttgcca ggcattcggc aagcggcaaa
     1021 tcttcggtac tgtataacgc tttttggaaa aaatcttcca aatccaagcc ggtaatttcc
     1081 tgacagcgga tttgccagtg tttttccgga atacccgagt gtgtgtccct ccactcccga
     1141 tagagtttgt ccattaacgt atcgagagaa tgtctgccgt tgcttcggtt gcgtattatc
     1201 agatcaaggc acaatgcggc aagcgcgcct ttctggtagt agctgacgat ggcgttgggg
     1261 ctgttttcat ccggtttgta aaatttgttc cacgcggtaa aactcgattc cgccaaggtc
     1321 tgcctcaaac ggccgcgggt ttgttgtacg cgcgtaatgc cttgtgccag caggtttaaa
     1381 taagattcgg gcgagatggt gcggctgcgt gccaaaaaca aatcgtcgta ataggatgta
     1441 ataccttcga atgcccatag ttgttcggta tagttttctt tgtcgaggtc ataagggacg
     1501 aacgcggcag gtttgatgga tttgacgttc cacgcgtgaa aatattcgtg ggagaaaagt
     1561 ccgagcaatg tggtgtaggt atcgtcggca tcggtcatac cgtacggcgg aaggctgtgg
     1621 cggtcggcga gcagggcggt gctgtcggtg tgttccaaac cgccgtaaat atggtcgccg
     1681 acgtggagca ggaacaaata tttttgaaac ggggcagggg aggaaaacac cgccagttct
     1741 gtttcgcaga tttttttgat atccgaaacc agcctgttgc ggtcgaaatc gggatatatg
     1801 ccgcttaagg caattgtgtg cggaatgcct gccgcctcaa aatctaaaaa ttcaatcaag
     1861 cccatctcga caggtcggtc aatcaattcg gcataagatg ccgcctgaaa gacaaacctc
     1921 cctgtttccg gcagcgttgt ggcaatacgc cattcggacg gaatacccgt caattccaag
     1981 cggtgcggca gcgtttccgt tccttcgact ttcaaaaaca ggcacgatcc gtcaaaaaaa
     2041 ccgcgttctg tcgtcaggaa agaacctcga accgacaaat cgaatgcata tacggtgtag
     2101 cggatttgcc actcgccgcg tacggcggcg gcatgccagc ggtttttggc aatttgttcg
     2161 agcggcatgg acgtgccgtt acaggatgca tggatagaag tgatgtggcg ggaaaaatcc
     2221 cgaatcagat agctgcccgg aacccagttt ggcaggctta tttctatagg aagatcatca
     2281 tcttgtgtga atgtcagcag gatgtgccac tcgtggtcga gaggggacgg ggttatttta
     2341 taatgaatca taactatcaa atatatcgtt aaacgggatg gattaaatac ctttaaaatg
     2401 cagcttaacc tacatcaata aagtgccaca aaaagggaaa attggaaaca aaagcaatgg
     2461 cggtcgggtt gtaatttatt ataacaatat gatttatatg taataaaaaa cagattaagc
     2521 aaagggctcg tcggcggcgc gaatgtaatt ccgcttggcg taataaggca gatatgttaa
     2581 atttagaaaa aattacgctt gcccggattg caccttaaac aggctgcgga agcggcagga
     2641 taaaaatctt tattcccata aattcatgga gactctcatg gacacacaaa cttacaacta
     2701 caaagtggtg cgccagttcg ccatcatgac tgtagtttgg gggattgtgg gtatgttggt
     2761 cggcgttatc gtcgccgccc agctttttgc tcctgccctc gacttgtcta atatcggacc
     2821 ttggttccac ttcggccgcc tgcgtccgct gcacaccaat gcggttattt tcgcatttgg
     2881 cggttgcggc ctgatcggca catcatacta cgttgtccaa cgtacttgta atacccgtct
     2941 tttcggcggt tggctgccgg catttacctt ttggggctgg caggcggtaa tcgttgccgc
     3001 cgtcgtcagc ttccctatgg gttggaccca aggtaaggaa tatgccgaac tggaatggcc
     3061 gatcgatatt ctgattactt tggtttgggt ggcttacgcc atcgtattct tcggtacgat
     3121 tgccaaacgt aagattaaac atatttacgt tgccaactgg ttctacggcg gctttatttt
     3181 ggcggtcgca cttttacaca tcgtcaacaa tatcagcatc ccggccggtt tgatgaagtc
     3241 ataccccgtc tattccggtg cgattgatgc tatggttcaa tggtggtacg ggcataatgc
     3301 cgtgggcttc ttcctgactg ccggcttctt gggtatgatg tactatttcg tacccaaaca
     3361 agcagcccgc cccgtttact cctaccgcct gtccgtcgtt cacttctggg cgttgatttt
     3421 cacctatatg tgggcgggtc cgcaccatct tcactacact gcgctgcctg actggacgca
     3481 atctttgggt atggttctgt ctttgattct gttcgcaccc tcttggggcg gtatgattaa
     3541 cggcatcatg accttgtccg gcgcgtggga caaactgcgt acagacccga ttcttaaatt
     3601 cctgattgta tccttgtcct tctacggtat gtctaccttt gaaggcccga tgatgtcgat
     3661 taaaacggtc aatgcattga gccactatac ggactggacc gtcgcgcacg ttcatgcggg
     3721 tgcgttgggc tgggtaggct ttgtaaccat cggttccgtc tattacatga ttccccgtct
     3781 gttcggcaaa gaacagatgc acagcaccaa gctggtagaa gcacattttt ggattgcgac
     3841 catcggcgtg gttctgtata tcgctgccat gtggattgcc ggtgtgatgc agggtttgat
     3901 gtggagttct ttgaacgatg acggtacgct gacctactcg tttgtcgaat ccgtaaaacg
     3961 caccatgcct tactacgtga tccgttttgc aggtggttta ttgtatctga gtggtatgtg
     4021 cattatggcg tacaacgtgt accgcacagc catcggtggt aaagcagtcg atgccgaaat
     4081 ccctgcggtt tcccaaacac agcaccacta aaatactaag aaagataggc taccaaaatg
     4141 aaattacaac aattggctga agaaaaaatc ggcgttctga ttgtgttcac gctgcttgta
     4201 gtcagtgtcg gtctgttgat tgaagttgtg cccttggcct ttaccaaggc ggcaacacag
     4261 ccggcaccgg gcgtgaagcc ttacaatgcc ttgcaggttg ccggacgcga tatttacatc
     4321 cgtgagggct gttacaactg ccactctcaa atgattcgtc cgttccgtgc ggaaaccgag
     4381 cgttacggtc attactctgt tgccggagag tcggtttacg accatccttt ccaatggggt
     4441 tccaaacgta ccggtcctga tttggcacgt gtaggcggcc gctattccga cgaatggcac
     4501 cgtatccacc tgctgaatcc ccgtgatgtc gtgcctgagt ccaatatgcc ggcattccca
     4561 tggcttgcac gcaataaagt cgatgtcgat gcaaccgttg ccaacatgaa ggctttgcgt
     4621 aaagtaggta ctccttacag tgatgaggaa attgcgaaag cgcctgaggc tttggcaaac
     4681 aaatccgagc tggatgctgt agtcgcctat ctgcaaggat tgggtctggc tttgaaaaac
     4741 gtaaggtaac atcatggata ttaacggtat tcgcgctctt ttcacggtat ggatctttat
     4801 ctgtttcctg ttggtactct atatcgtctt caacaggcgg aataagaaaa actacgataa
     4861 tgccgcaaac agcatttttg atgaaaacca agatgcgcaa gataagaaaa gcgaaaaccg
     4921 ttaatattgt gataacggag caaaacaatg aacacaacat cccaatttac cagtaatttc
     4981 tggaatatat atattgcagt tattgtctta ctgagcttta tcgctttggc ttggctgctg
     5041 ctgtctcaaa atgttgtcaa acgtccgaag aagggcgaag aagtacaaac tacgggtcat
     5101 gagtgggacg gcattgccga atacgacaat ccgctgcccc gctggtggtt ttggctgtgt
     5161 gttttgacgt ggctgttcgg tatcggttat ttggttatgt atccgggtgt cggcgactac
     5221 aaaggtctgc tgaaatggac cagccataac caatatgaaa aagaggtcaa aaaagccgat
     5281 gagcaatacg gcaaactgta tgccaagttt gcggatatgc cgattgaaaa agtggcaaaa
     5341 gaccctcaag ccaagcaaat cgcccaaaac ctgtttaaca cttattgtat ccagtgccac
     5401 ggctctgatg ctaaaggctc taaaggtttt ccgaatctga ccgatagcga ttggttgtgg
     5461 ggcggtgatc ccgataaaat ccacgagacc atcgaaaaag gccgtgttgc gactatgcct
     5521 gcctggggtc ctgctttggg cgaagaaggc gtgaaagacg ttgcccatta tgtgatgtcc
     5581 ctttcaaaac ccgaaggtca gtatgatgag gaacgtgccg cgcgcggaca agccttgttc
     5641 agcggtccgc ctgccaactg tttcacttgc cacggcgata agggacaagg tatccaaggt
     5701 ttgggtccga acctgactga tgacgtgtgg ttgtggggcg gtacgcaaaa atccattatc
     5761 gaaaccatta ccaacggtcg cagcagccaa atgcccgctt ggggacattt cttggataaa
     5821 gacaaactgc atattatgac tgcttatgta tggggtcttt ccgataaaga cggtaaagct
     5881 ccggtgaaaa aagccgagcc tgcaccgact cccgcaccgg cggcagaacc cgcagcctct
     5941 gctcctgcag aagcagcaca agccgcgtcc gaagccaaac ctgccgcagc agaacctaaa
     6001 gccgaggaaa aagctgcacc tgctgccaaa gcggacggca aataggttta tgaaaccgtt
     6061 tgtgccgcct gccatggcaa tgcgattccg ggtattcccc atgtaggcat caaagccgat
     6121 tgggccgacc gcatcaaaaa aggcaaggac acgttgcaca aacacgcgat cgaaggtttc
     6181 aatacgatgc ccgcaaaagg cggtcgcggc gatttgagcg atgatgaggt taaagctgcg
     6241 gttgactata tggtcaacca gtcaggcggc aaattctaac ttgactgagt ttccgatatt
     6301 atcggttatg aaaatccaga ttcagtattg aatctggatt ttttgtttta acgggcatcc
     6361 ggttttaaac gcagactgcc tatttgttgt aatgaatttt gactttagtc ataaagaatt
     6421 agtctttatt gtttataatc gcgcatcatc acgatagccc tattttcaaa aggacgatta
     6481 atggatacac aaatcaaaac tgaagccgac aatcagagca accggcgtta tctgaccgtt
     6541 tggcggtggc atttttatgc cggtctgctg gttatgcctt ttcttaccct gcttgccgtt
     6601 acgggtttgg gtatgctgct gtttgccaat attaccggta aggagggcga gcggattcat
     6661 gttgtgccgc aggcaacggt acaacctctg tctgttcagg cggaagcggc acgcagtgcc
     6721 gttaatccgg aaacttcgtc cgtcgttcag tatattgcgc cgcgtgccga tgatatggtt
     6781 gccgtgttcc gtgtcaacaa tgagggcaag gcaacgatgg tcgcggtcga tccttatacg
     6841 gcaaaagtgg tcagtaccat gccgcgcaat cagggttggt attacacgat ggatgaaatc
     6901 cacagcgata tgatgctcgg tgcggcaggc gattatcttt tggaaacggc agcatcgctg
     6961 acgattatca tgataatcag cggcttgtac ctttggtggg tgaaacggcg cggcatcaag
     7021 gcgatgctgc tgccgtcaaa aggcagggcg cgttcttggt ggcggaatct gcacggcacg
     7081 tttggaactt gggtgtcgtt gattttgctg ttgttctgcc tgtcgggtat tgcttgggcg
     7141 ggtatttggg gcggcaagtt cgtacaggct tggagtcagt tccctgccgg taaatggggt
     7201 gtcgaaccga accccgtttc agtcgtgccg acccacggcg aggtattgaa tgacggcaag
     7261 gttaaggaag tgccgtgggt tttggagctt acgcctatgc ctgtttcagg gacgactgtg
     7321 ggcaaagacg gcattaaccc tgacgagccg atgacattgg aaaccgtcga ccgctttgcg
     7381 cgggaaatcg gtttcaaagg gcgttatcag ttgaatttgc ccaaaggcga ggacggcgta
     7441 tggactttgt cgcaggattc tatgagttac gacatgatca gcccgtttgc cgaccgcacg
     7501 gtacatatcg accagtacag cggcaaaatc cttgccgaca tccgttttga cgattacaac
     7561 ccgttcggca aatttatggc ggcaagcatt gcgctgcata tggggactct gggctggtgg
     7621 agcgtgttgg cgaacgtctt gttctgcctt gccgtcattt ttatcggtat cagcggctgc
     7681 gtgatgtggt ggaaacgccg tccgaccgga gcggtgggca tcgttccgcc ggcgcagaaa
     7741 gtcaagctgc cggtttggtg gatgatggca ttgccgctat tggcaatcgc actgctcttc
     7801 ccgaccgcac tgcttgccat tgccgtgatt tggctgttgg ataccttgct gctgtcgcgg
     7861 attcctgttt tgaggagatg gtttaaatga cccgatgccg tctgaagcgt tcagacggca
     7921 ttttgtttga aggcgggcgg gggaaaggct atataatccc gaatacttga ccacagcttc
     7981 tgtttgaaat catgttttat ctgtatcaat ccaaccgtct tgaaacgctg gcggcactgt
     8041 ttgcccgcat tcaaaaagtc aaaccgctga aatcggtttt acagcccgaa cagattattg
     8101 tgcagagtca ggggatgcgc cgctacctca atacctgcct cgcccgcgat ttgggcgtgg
     8161 cggcgaattt gtcgttcagc ctgcccgccg gtctgacgtg gaagctgatg aaaaaactga
     8221 ttcccggtat tccggaactc agcccgtttg cgcctgaagt gatgcgctgg cggctgttgg
     8281 atttgttccg cagcgaggca tttcggaata cggcagaatt tgaagatgtg aggaatgtgc
     8341 tgcaagacta tctgggcagc ggcgaatcgg cagattatca gcttgcggga cagcttgcgg
     8401 acatattcga ccaatacctc gtgtaccgtc ctcagtggat agacgcttgg cagcagggca
     8461 ggcggctcgg tttgggcgac gacgaaatct ggcagtccaa actgtggcgt tacctcgacg
     8521 atggcaggca gagcgcgccg caccgtgtcg cgttgtggga aaagctgttg gaatctttaa
     8581 gcagtgataa gctgcccgag cgttatttcg tgttcggcat ttccacgatg gcaccgatgt
     8641 atttgcagct tttgcacaag ctgtccgaat attgcgatgt gtttgtgttc gcactcaatc
     8701 cgagcgggat gtattggggc aatgtcatcg aagcggcgca aatcctcaaa ggcggcggcg
     8761 atcccgattt aactcaggca gggcatccgc tgctcgcctc attgggcaag caggggcggg
     8821 actttttcga ctttttgaac gaaatggaaa tagaaggaga aacgccggta tttgaagaag
     8881 gcgggcgcga tacgcttttg cacgccctgc aaaccgatat tcaaaacctg aaaatgccgt
     8941 ctgaaaatgt gggaagcgtc aacacgggcg acggctcgat acgcatcgtc tccgcacaca
     9001 gccccttgcg cgaattgcag atactcaaag acaagctgtt gaaaattctg catgaacatc
     9061 ccgattggca gccgcatgat atcgccgtat taaccccgaa catcgaatcc tatacgcctt
     9121 ttatcgaagc cgtgttcgga caggcgcagc ccggtgcgca ggcattgccg tattccgttt
     9181 cagacgtgaa aatcagccgc cgacaaccgc tgtttcatgc tttgtcatgc ctgttcgact
     9241 tgttggaaag ccgatttgaa gtcgataaag tgcttgtgct tttagaaacc gcacccgtgt
     9301 tgcgccgttt cggactgact gaggacgatt tgccgctttt gcacgatatg gttgccgatt
     9361 tgaacgttca ctggggtttg gacggagaaa tgcgcggcgg cacggatcag ctgtttacct
     9421 ggaagcaggc ggtagaacgc atgatattgg gctggatgct gcccgaaggc ggcaacccga
     9481 tgtggcagga tgtcagcgcg tggtatgccg acgtgaatca aaccgccctg ttcggacgtt
     9541 ttgccgccct cctcgaaacc ctgtcggata ttgtacggat atggcggcag cccgcaacga
     9601 tcggcgaatg ggtggcgcgt tgccgggatt tgcttgaaac attgttccaa gctgaagccg
     9661 atgaccaaaa gtcagtccaa aaccttgaaa acgaatgggt caaatggcag gcggaaacag
     9721 aattggcgca attttccgga cagttgccgc aacaaaccgt catccgccat atccgacgtt
     9781 tcctcgacag cgaaagcgag gcaggctttt tacgcggcgg catcaccttt tgcagtatgg
     9841 tgccgatgcg gagcctgccg ttcaaagtca tctgcctgtt gggtttgaac gacggagatt
     9901 ttccccgcaa taccaaagcc gccgtattcg acctgattgc caaacatccc gccaaaggcg
     9961 accgcgcccg ccgcgatgac gaccgctacc tcttcctcga agccctcatc agcgcgcgcg
    10021 aaatcctcta cctgtcctac atcgggcgcg acatccgcaa agacgaagag cttgctccgt
    10081 cttcgctgtt gggcgaactc atcgacacag ttgccgctat ggcgggcatc ggtagccgcc
    10141 aacttgcaca aaactggata gaacagcatc cgctgcaagc cttctcgcgc cgatattttc
    10201 aagaaggcgg acgttcagac ggcatattcg gcacgcgtac cgactacgcc actgcgctcg
    10261 gacaaacgcc cgaaccgccg caaccctttt tcgaccaacc cgtagaaaac gccgaacctg
    10321 ttgccgaaat cggacaggac gaatttatcc gtttctggcg caaccccgtc aaagtatggc
    10381 ttcagcagca gcttgcgtgg agcgaacccc atatcggcga agcctgggag cctgccgaac
    10441 ccttcgagtc gcaacacgcc gatcaaatcg ccgaaaccta catcgaagca cggcgcgaag
    10501 gacgggattt tgcccaaacc gccgcccgca tcggggcgga aagcctcctg ccgtcgggag
    10561 agttgggcag actttggcag cagaacttcc aaactgctgc caaacaaatc gacacggcgg
    10621 ttttaaacag ccccaaactg ccaccgcttt catatgccat accgtcggac gggcaaatcc
    10681 tgaaaggcag cttgggcaat ctgtaccgct gcggacaagt gttttacgcc tacggcaaac
    10741 ccaacgcgcc gcaacgtatc gcttttctgc tggaacacct gatattttgc gccgttatgc
    10801 cgtctgaagc cgaaacgcgg caaaccttta tcgtccaatc cggagaaacc gaaatattgg
    10861 cggaaatcgc gcaagacagg gcattgcagc tattgtcgga atggatggcg tttttcaata
    10921 tcgggcaaaa ccgcccgctg ccgttttttg ccaagacctc gcttgccgct gccgaagcgt
    10981 ttgcccaaaa acaagattgg gaagccgccc tgaaaaaagc ccaaaccgcc tattacggca
    11041 acaaagtcag caaagggcag aaagactata ccgaagtcgc cctcgtgttc ggcaacgcaa
    11101 gccaaaaccc actcgaacaa cccctgtttg aaaacctcgc ccgcctgctt gccgacacgc
    11161 ttgccgcagc ggaaaaaaag gaagaggccg gagaagcctg acgggataga aaggcaaatg
    11221 ccgtctgaaa gcgtttaagc atttcagacg gcattttgtt tatcgtgttt aaggtgcggg
    11281 atagaatgtg ggcgcaccgg gaccgacggg caggcccaaa acaaataccc aaatgcagaa
    11341 taaggcaatc catgcaatta agaagaaagc ggaatacggc aacatcatag aaatcagcgt
    11401 acccacgccc gcatcttttt tgtatttgat caccgtcgcc ataatcagcc cgaaataact
    11461 catcatcggc gtaataatat tggtaacgga atcaccgatg cggtaagcgg cttgaatgac
    11521 ttcgggcgcg tagccggcca acatcagcat agggacgaaa atcggcgcag ttaccgccca
    11581 ttgcgcggag gcggagccta tcatcagatt gataaaagca caaattaaaa taaaaccgat
    11641 aaacaacacg ctgccgccca agccgacttc ttttaagaac gtcgcccctt taacggcaat
    11701 atattgccca atattcgtcc aattaaaaaa tgcgacaaac tgtgcggcaa aaaagatgat
    11761 gaccaaataa agccccagag tactcatcga ttcggccatc gcattaacga cttcctgttc
    11821 gccgcgcaaa cttcgggtta cccggccata aacaatgccc ggcagcgcaa acaacaagaa
    11881 aataaaaaca acaatcgatt ttaaaaacgg cgaaccggaa accaatcctg tttcaggatg
    11941 acgcaaaata ccgtcggcag ggacgatgct ccaagccaat agggcggata aggcaacaaa
    12001 caccacgcca gcccaaatta atcctttata ttccaaaggc gtgatttcat tggaatgccg
    12061 aatgtctttt tcttcttgtg acaaatctga ttgataaggg cccaattgcg gttcgacgat
    12121 tttttcagta acaaaataac caatcaaagc aatcacaaac gtactggcta ccataaaaaa
    12181 ccagttggct tcagggccta cgacgtagtc gggatggatg atttgcgccg cctgttgggt
    12241 gatgcctgcc aagagcggat cgattgtgcc taagaacaga ttggccgaat aaccgcccga
    12301 aacgccggcg aaagccgcag ccaaaccggc aagcggatgg cggccgagcg aatgaaagat
    12361 gacggcggac aaagggatta ggacgacata gcccaattca gaagcggtat tagataaaat
    12421 ccctgtaaaa acaaccataa aagtagtgag tttgcgtgga gattttgtga gcaataagcg
    12481 cattaatgcg gaaatcaagc ccgatttttc cgcaatcccc acgcccaata aagaaaccaa
    12541 caccgttccc aacggcgcga aaccggtgaa atttttaacg gtatgcgtca ggattttgat
    12601 caaaccgtcg gcattgagca ggctgacaac gtaaatcaaa ccgtcatcgg cacgtccttt
    12661 cgcaccaaca gggcgcggat cggggacgga tagtccgaaa tacgcaccga cggcagaggc
    12721 aatcagcaat aacacaatga aaataataaa aagcgtaacc ggatgcggca acatattgcc
    12781 cagccattcg actgtgcgta aaaatcgccc gtcccgttgc gtatcggttt gactcatctg
    12841 cttctcctta aaaaaaatat attgagtgca ttaaattttt gaatgtaaca tgaagtgttt
    12901 tgtaagcaaa ctaaaataat cgtatatgta tataaccgta atagattaaa acagaaaatg
    12961 cccacatcgt tattctcata aaggcggtaa tccgaaaact tgacgttaaa gccttatcag
    13021 gaatgactgg aactcaaaaa aacggattcc cgcctgcgcg ggaatgacgg gattttcggt
    13081 ttttgtttgc aagaatgccg gagaagaatg cagtttacat tttgaatggt attgttatta
    13141 attgtaaata aatattggaa ggtaaatgat atgaaaaatg aaattcaaaa aattatggac
    13201 aaatatgatc cctggcatga agatgatttt gagtcgtatg aagacattgc cagagatgta
    13261 tcgctgatga cagataaaac gttcattgaa cattatttgt tagaagttta ttcagaagaa
    13321 aacggacatt ttgaccaaga gaatgtccat gcaatgatag aagaaattaa aaatgcaatt
    13381 tagtgaa
//
LOCUS       DAXHTR010000052        12865 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.052, whole genome shotgun sequence.
ACCESSION   DAXHTR010000052 DAXHTR010000000
VERSION     DAXHTR010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12865
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.052"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(53..361)
                     /locus_tag="ACFNML_000996"
     CDS             complement(53..361)
                     /locus_tag="ACFNML_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392402.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(345..647)
                     /locus_tag="ACFNML_000997"
     CDS             complement(345..647)
                     /locus_tag="ACFNML_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFC6392403.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(721..3084)
                     /gene="pheT"
                     /locus_tag="ACFNML_000998"
     CDS             complement(721..3084)
                     /gene="pheT"
                     /locus_tag="ACFNML_000998"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFC6392404.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGGLVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSDGMLCSTDELGLPDDGVNGLHILPEDAPVGTNIREYLDLDDTLFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQIDAPADCGRFI
                     SRVIENVNARATTPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLGRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     RVFEIARVFGKGSDGRFVQNERIGGLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGNVIGFVGELHPKWLQKYDLPQAPLVFEIDMAAV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3120..3638)
                     /locus_tag="ACFNML_000999"
     CDS             complement(3120..3638)
                     /locus_tag="ACFNML_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392405.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3645..4709)
                     /locus_tag="ACFNML_001000"
     CDS             complement(3645..4709)
                     /locus_tag="ACFNML_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFC6392406.1"
                     /translation="MIKSFELENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="ACFNML_001001"
     CDS             complement(4886..5878)
                     /gene="pheS"
                     /locus_tag="ACFNML_001001"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFC6392407.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGLLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLKNVNIDSEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="ACFNML_001002"
     CDS             complement(6224..6583)
                     /gene="rplT"
                     /locus_tag="ACFNML_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFC6392408.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGARENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="ACFNML_001003"
     CDS             complement(6596..6793)
                     /gene="rpmI"
                     /locus_tag="ACFNML_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFC6392409.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6940..7461)
                     /gene="infC"
                     /locus_tag="ACFNML_001004"
     CDS             complement(6940..7461)
                     /gene="infC"
                     /locus_tag="ACFNML_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFC6392410.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="ACFNML_001005"
     CDS             complement(7479..9392)
                     /gene="thrS"
                     /locus_tag="ACFNML_001005"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFC6392411.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLARAAVAGRVNGKL
                     VDVCDPIVENSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVSVKLSLRPEKRAGSDDVWDKAEQGLREALTACGVE
                     WGELPGEGAFYGPKIEYHVRDALGRSWQCGTLQLDFVLPERLGAEYVTENNDRSRPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9446..9522)
                     /locus_tag="ACFNML_001006"
     tRNA            complement(9446..9522)
                     /locus_tag="ACFNML_001006"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9486..9488),aa:Val,seq:gac)
     gene            9701..10816
                     /gene="tgt"
                     /locus_tag="ACFNML_001007"
     CDS             9701..10816
                     /gene="tgt"
                     /locus_tag="ACFNML_001007"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFC6392412.1"
                     /translation="MLKFTLHKKDGYARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQARKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLKGLEELDFPGLAIGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGVDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            10826..11908
                     /gene="hemH"
                     /locus_tag="ACFNML_001008"
     CDS             10826..11908
                     /gene="hemH"
                     /locus_tag="ACFNML_001008"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFC6392413.1"
                     /translation="MPSEAASDGILPLYPTRTIFQKPIMLPFFPEPSLSYTQQNRTAV
                     LLLNLGTPDAPTAQAVRPYLKSFLTDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYE
                     KIWFKEGSPLEVYTARQAAALAERMPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLV
                     IPMYPQYAASSSGAAVDKVCEQLLLQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAE
                     HGRGKKLMLSFHGVPQKHYDLGDPYPDECRHTAKLLAEALELTEDQYVVSFQSQFGRA
                     KWVTPSTQDLFGKLPKQGVTELDVFCPGFLADCLETMEEIALMGREQFYEAGGKSYRY
                     IPCLNDNPDWIDALVALAEENLGGWR"
     gene            12100..12501
                     /locus_tag="ACFNML_001009"
     CDS             12100..12501
                     /locus_tag="ACFNML_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6392414.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(12564..12854)
                     /locus_tag="ACFNML_001010"
     CDS             complement(12564..12854)
                     /locus_tag="ACFNML_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392415.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGDGNIGSMGLKVPR"
BASE COUNT         3254 a         3407 c         2866 g         3338 t
ORIGIN      
        1 attttttagc gtgtaaaaca aaaccggctg catacctgca accggcctca aatcagcaca
       61 attccttatc caaatccgcc aacaggtctt tcagaccgtc tccgatttcc tgaagcgtaa
      121 ccggacggtt gccgtccgaa cccgattccg caccttctgc atacggtgca aacgtgctct
      181 gcaatttcaa cagtttcacc acatccaaac gggtgtagcg ttcgccgccg taaccgacaa
      241 ccacaccgtg attatgctgc cattgcgcaa aaccataggg gctgatttgc aacagtccgc
      301 acaactcgtc cagcgtgaaa tagcgttttg cgggaacctt caaatcaacg ttgtttgtca
      361 tagtagtgtt ccaccatgct tttgagtttc tggctggcat ggaaagttac cacgcggcgg
      421 gcggtaatcg gcacttcctc gccggtttta ggattacgac ccgggcgttg cggcttgtcg
      481 cgcaactgga aatttccgaa accggaaatt ttgatttctt cgccgcttgc caaagtgctg
      541 cggatttctt caaaaaagag ttcgacgatt tctttggcat cgtttttggt gacgttgctg
      601 actttgtcta ccaaaatatc ggccagttct gctttagtga gagtcatatg attaccttct
      661 gttgtaacag ttgtggatta ttaccaattt ttatttaaaa ttcaagcgaa tatttatttt
      721 ttaactgcga agccgcgccc ctgccgccgt tgccgcgcca atcagttttc cgataagcgg
      781 ctcgaccgcc tcatccgtca gcgtgttttc catatcctgc aaaatcactt tgaccgccac
      841 gctcttcatc ccttcgggca gtcccgtgcc gcgatacacg tcaaacacgc tgatttcctg
      901 taccaacttg tttgccgcgc ctttcaagac aagcagcaaa tcatcatggc tcatagcttc
      961 cggcatcaca aacgccaaat cgcggcgcac cggctggaat ttcgatacgg cccgatagcg
     1021 cgttttcccg cattccaaca cggccgccat atcgatttca aataccagcg gcgcttgcgg
     1081 caggtcgtat ttttgcagcc atttcggatg cagttcgccg acaaagccga tgacgttgcc
     1141 gtctgaaacg atattggcgg cgcgtccggg atgcagggca ggatgtccgg ttttaacgaa
     1201 ctcgaccgct ttgtttttca acagattttc cacatctgcc ttaatgtcgt aaaaatccgc
     1261 attgcgcgtt ttcccgcccc attgttcggg cattgccgcg ccgtaccaca agccgccgat
     1321 gcgttcgttt tggacaaacc ggccgtctga acctttgccg aacacgcggg cgatttcaaa
     1381 cacgcgcacg cggttttgtt tgcggttcag gttgttttgc aaaatttcca ccaagccgcc
     1441 gatgagtgtg gaacgcatca cggcatattg cgccgccagc gggttttgca ggcggatggg
     1501 gtcggcgttg gcggcaaaat cttgttccca ctgctcgtca acgaaggcat agctgaccac
     1561 ttcgcggtag ccgcgcgccg ccatttcgtt gtaaacggca aaacgcgggc ggcgtgtttc
     1621 gggcagttcc agcattttca gacggcctga cgtgtaatcg tcggggatgt tttcatagcc
     1681 gtaaacgcgt ccgatttctt caatcaaatc agcctcaatt tcgatgtcaa aacggaagct
     1741 cggcgcggta acgcggaagc cttccgccgt tttctcgggc tgcaggccca agtgttgcaa
     1801 aatggtttcc acctgttcgg caggaatgtc cacgcccaac acggttttca gacggcccaa
     1861 acgcaatccg acctgcttcg cttcgggcaa ttcgccttgc gcttccacca tttcgcctgc
     1921 cgcaccgccg caaatctgca acaccaattc ggtagcacgc tcaatggcat ccgcctgcaa
     1981 acggtaatcc acgccgcgct cgaagcggaa cgacgaatcc gaaccgaaac cgtattggcg
     2041 cgatttgccg gcgatgattt cgggcgcaaa ccaagccgct tccagcacga tattttgcgt
     2101 gccgtctgaa accgcgctcg ccgcgccgcc cattaagccc gccaaactca acacgccttt
     2161 ttcgtccgcc acgaccagcg tgttttcaga cagggaaacg gttttctcgt tcaggcattc
     2221 cagcgtttcc ccttcgcgcg cgcggcggat gtgcaggctg ccggaaagtt tgtcggcatc
     2281 aaaaacgtgc atcggctgac cgatttccag catcacataa ttgccgatgt ccaccagcgc
     2341 ggaaatactg cggatgccgc tgcgctccaa acgttgtttc atccaatccg gcgtagtagc
     2401 gcgcgcgttc acgttttcaa tcacacggct gataaagcga ccgcaatcgg caggcgcgtc
     2461 aatctgcacg ggctgttttc gactgcccgt gatcggcgcg gtatggattt cgggctgcct
     2521 gaacgcgcac cccgtcaatg cggacacttc gcgcgcaatg cctttgatgc tcaagcagtc
     2581 ggcgcggtta ggcgtaattt tcaacgtaaa cagcgtatcg tccaaatcca agtattcgcg
     2641 gatattggta ccgacgggcg catcttcagg cagaatgtgc aggccgttca caccgtcgtc
     2701 gggcagaccg agttcgtcgg tggaacacaa catcccgtcc gacaccacgc cgcgcatttt
     2761 ggtcggcttg attttgaaat tgcccggcaa cactgcgccc ggcaacgaac acggcacttt
     2821 gatgcccgct ttcacattcg gcgcaccgca cacaatctgc accaacccgc ccgtacccgc
     2881 atcaactcgg gtaacgttca aacggtctgc atccggatgt ttttcaacgg atttcacttc
     2941 ggcaatcacc acgcccgcaa acgcaggcgc ggcagtttcg gcctcttcca cttccaagcc
     3001 ggacatcgtt aacagatgtt ccagcttatc ggaggaaagt tcggtatcgg cttgggtttt
     3061 cagccatgaa taggagaatt gcatgttatg ttctcaatca attaattaac tttagatttt
     3121 caagagctag ctaaaccttt ttgaatggct aagtccctat taattacttc tacagaaatg
     3181 ttccataaag aaatcaaccg caaagtatct ttcttattaa attcttgctt tattaattca
     3241 ttcaattgcc caagataatt aagcggcaca tccgggataa ccagtaataa tttgatttct
     3301 atgctacccc attgcaaatt tgaaaaagca ctaggcataa ttgtttcttt gatatcttct
     3361 ctgcctaata ttcccaaaat ttcaacattt aatgcattac accatttttg ataaatctct
     3421 gaaatatatt catcaaaacg ctcaggagat ctttcttgat ttggaatact ttgttttgcc
     3481 tcaacaaata acaaattatc ttcttgcaga tagataaact ctactgtctt aataccttct
     3541 tgccttctct tccggatata tggttctata taaaacaggt tattttttac tagattaaaa
     3601 gttatacctg attcagtgag atttattatt tcactcataa aaacctaacc aatccatgct
     3661 gtttccggag ataaaacacc tatttcttct ctgaacaatc gaatagactc accaataatt
     3721 tcattatccg gcatgctatt ttgtaaattt tcctgcaccc aagtagtctg ttccttttct
     3781 ttgccaggca taaaaacagg aatactttca ctatgctttc tagctaataa acacagcttc
     3841 ttgataacaa aatatgaatg cgtactcata aaaatctgta ttcccaattt ggatttagat
     3901 aaggaaaaca gaatttctag aaattgatta attgctttcg gatgtagatt agcttctggc
     3961 tcatcaataa ataagattga tccaggactc aaaaaccgat ttcctaatag agtgtctaat
     4021 atcccaatct tttttacccc ctctgctgta gaattgatag agaaagtttg tctatctttt
     4081 tcatataccc atgccttctt tttatcattg tagctaacat aaccgccaaa caatgcttct
     4141 agatcctttc tagcttccgc tgcctcaggg taattcctcc cttttgttgg tttaatatcc
     4201 aatgctttta ccaaatcaat gtaagtagca tcaaaaccaa atctcttttc ttcctctgat
     4261 tttttaatta catcaaatag tgatagaact tctttgggtg gtaaaaaaat agagttagcg
     4321 tttattctgg gagaaagttc gtatagctcc ggtttaatta gctttttggt agttctacca
     4381 aaagaaaaca aaactgaaga actatcttct aaagtcatac ttaacttaag aggccgttcg
     4441 tttggatttt cttttcctct tgtaactaaa tcaccaattt cctctacttg gaacgtccaa
     4501 tatagcttat ctcgcaattc ctcagaaaaa tcacgaatat tctttccttt atgagcttcc
     4561 tcatgacttt ttaaaacact atataatgcc tttaaaataa aagttttccc tttgctattt
     4621 tctgcaataa ttaaatttat tttccctaaa ttatcagcct tcaataagct aatcggcccg
     4681 aaattttcaa gctcaaatga tttaatcata taatccccct aatatatatc aacaatattt
     4741 gtacaagcca cattgtaaaa agacagcaac cacagcacca tagtaaacgc atagcaggcg
     4801 gataatggag cgacatagcg tttcatgatt tagctttctg aggtaggcac aggctgcttt
     4861 tttcattttt aggcagccta cacaatcatt ttacaaactg cttcaaaaaa ttcaaatcgt
     4921 tatcaaaaaa caagcgcaaa tcgttcacgt tgtaacgcag catcgcgaaa cggtcgagac
     4981 cgataccgaa agcgaaaccg gtgtattttt cagaatcgat attcacattt ttcaacacgt
     5041 tgggatgcac catgccgcag ccgccgactt ccagccattt gccgttttct cccataatgt
     5101 cgatttcggc agacggttcg gtgaacggga agaaagacgg gcggaaacgt acttgcaaat
     5161 catcgcgttc aaagaagcga cggataaaat ccgtgaacac tgcttttaag tcggcaaaag
     5221 ttacgccctc ttctacccac aaaccttccg cctgatggaa cataggcgag tgcgtggcat
     5281 cgctgtccac acggtaaacg cggccggggg cgataatgcg gatgggcggc tcttttttat
     5341 cgagcatata gcggatttga atcggggaag tgtgcgtacg caaaacatca ccgttttcaa
     5401 cgtaaaacgt atcctgcatc gcacgggcag gatggtttgc agggatgttc agggcttgga
     5461 aattgtgaaa atcgtcttcg atttcaggcc cgtccgccac ttcgaaaccc attccgtgaa
     5521 agagttcgac cacacgttgc aaggtcaggg ttacgggatg caggctgccg ccttcctgag
     5581 cgcgtccggg cagggtaatg tcgagggctt cggcggcaag tcgggcttgc agcttggctt
     5641 cgttgagggc atcgcgtttg gcattaaaag ccgtctgaaa ccggtttttg cattcattga
     5701 tatgcgcacc tatggttttg cgctcttcag gcgacatttg ccccaaagtt ttcagaagtc
     5761 cggtcaactc gccggttttg ccaagataac gggctttgat ttgttctaga gcgttgaagt
     5821 cttgcgcagc ttctactgcg gcaatgcctt ctgcaacgat gcggtttaca ttttccataa
     5881 tatcaagtct gtcaattaat gatatgccac ctgaaacaac gaggccgcct gtatagcctg
     5941 acatatcaga ctcttcagac gaccttattt tcttcacggt caatccattg gataaatttt
     6001 ctgtatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgccg tactatctgt
     6061 actgtctgcg gcttcgtcgc cttgtcctga tttaaattta atccactata ttttaacgca
     6121 aatttattta aaaaaataga ctttaaagaa agaaaatcaa tcacatagaa tttctgcaaa
     6181 caaagaaaaa aggaagccgc agcttccttt tcaatttttt ggattaagca gccaaagcag
     6241 ctttggcttt ttcaaccaat tgtgcaaaag cggctttatc gaacacggcc aaatcagcca
     6301 atactttgcg gtcgatttcg atagaggcgc gtttcagacc attcataaat ttgctgtaag
     6361 acaacccgtt ttcacgcgca cctgcattga tacggacaat ccacaattga cggaattggc
     6421 gtttgcgttg gcggcggtcg cggtatgcgt attggccggc tttcattacc gcctgcttgg
     6481 caacgcggta aacgttttta cgacggccgc ggtagccttt ggctaacgcg aagatttttt
     6541 ggtgacgggc acgggcggta acaccgcgtt ttacgcgtgg catattctaa actccttaag
     6601 cgtagggtaa cattttagca acagaagcca aatcgcgatc atttaccata gaggtaccgc
     6661 gcagttggcg tttgtttttg gtggtctttt tagtcaagat gtggcgtttg aacgcatgag
     6721 cgcgtttcac accgccgtta cccagtactt taaagcgttt tttcgcgcta gacttggttt
     6781 tcattttagg catgggaaaa ctccattcgt tatcagataa ggcattaggg ggttttaaaa
     6841 catcggtttc aaacacttga acccacaata ccacggtttt ttgccgcaat tgaaaactta
     6901 ctccgaagcg gcaatcggag taagcggaga attatagctt tattttttct tcggcgcaat
     6961 catcatcacc atttggcgac cttccatttt gggaaaggac tcgatttgcg ccacttcagc
     7021 caaatcttct ttcacacgtt ccaaaagttg cgcgccgagt tgctggtgag ccatttcacg
     7081 gccgcggaaa cgcaatgtca ctttgacttt atcgccgtcg gcaaggaagc ggttgatgtt
     7141 gcgcatcttg atttgataat cgccctcatc cgtacccgga cggaatttga tttccttgat
     7201 ttgcacctgc ttttggtttt tcttggcttc gtcgcgtttc ttggcctgct ggtatttgta
     7261 tttaccgtaa tccatcagtt tgcacacagg cggtttagca gttggggaaa tctctaccaa
     7321 atcgacatcc tgcccttcgg ccatagccaa agcttcacga actgaaacaa caccaagctg
     7381 ttcgcctgac tcactgatta aacgcacttc tttggcggta atttcgccgt tgattcgtgc
     7441 ttcgcgttct tgagcgatga tgaatactcc tataaaaatt aatgattgac gagggcatca
     7501 gtgatttctt gctgcaattg cgcaatgaaa tcatccaaat ccaaagaacc caaatcttct
     7561 gctttgcggc gtaccgccac tttgttttcc tgcttctcct tatcgccgac aacgatttga
     7621 taagggaaac ggtattggct gttgtcgcgg attttgtaac cgattttttc gttacgcaaa
     7681 tccaactcgg cgcggaatcc tgccgcctgc aatttggcag ccacttcccg acaataatct
     7741 gcctgatttt cggtaatatt cataatcacc atttgaaccg gagccaacca taacgggaaa
     7801 gagcctgcat ggttctcaat cagaatgccg ataaatcgct ccaaagaacc taaaatggcg
     7861 cgatgcaaca taacaggtcg cgagcggtcg ttgttttcgg ttacatattc tgcacccaag
     7921 cgttccggca agacgaaatc cagttgtaat gtaccgcatt gccaagaacg acccaaggca
     7981 tctctgacat gatattcgat tttaggcccg taaaacgcac cctcgcccgg caattcgccc
     8041 cattccacgc cgcaggcagt caatgcctcg cgcaaaccct gctctgcctt atcccacacg
     8101 tcatctgaac ctgcacgttt ttcagggcga agagaaagct tgacggatac atcatggaaa
     8161 ccgaactgtt tgtagatgcg aaccaacaat tcattgaacg cacgggcctc gctgacgatt
     8221 tggtcttcgg tacagaaaat atgcgcatca tcctgcacaa atccacggac ccgcatcaga
     8281 ccatgcaatg caccgctcgg ctcgttgcgg tggcaggaac cgaattccgc caaacgcatc
     8341 ggcaaatctc gatacgaacg caaaccgttg ttaaaaattt gaacatgacc cggacagttc
     8401 atcggtttaa ccgcatattc gcgtttttcc gaactggtta cgaacatatt atctttgtag
     8461 ttgtcccaat ggccggattt ttcccaaaag gttttatcca tgatttgagg cgttttgacc
     8521 tctttataac cggcggcgtt cagttcttta cgcatatgct gttcaatcac ttgccacaaa
     8581 gcccagcctt taggatgcca aaacaccatg cccggcgctt cgtcttgcag atggaacaga
     8641 tccaattgct tgccaagctt gcggtggtcg cgcttttcgg cttcttcgat acgttgaata
     8701 taggctttca actcgtcttt tgtcgcccaa gccgtaccgt atatgcgttg cagcatttca
     8761 ttattgctgt cgccgcgcca gtatgcgccc gccagcttgg tcagtttgaa gtttttcagg
     8821 aaacgggtat tcggaacgtg cggaccgcgg cacatatcga catattcctg gtgatgatac
     8881 atccccatcg cttccacttc gggcatatcg tcaatcaggc gcagtttgta ttcttcgccg
     8941 cgctcttgaa aaattttaat cgcctccgca cgcggagtca tgattttgac cacatcatag
     9001 tcttgggcaa tcaattcttt catacgcgct tcaatggcgg caacatcttc cggtgtaaac
     9061 ggtttttccg tggcgatgtc gtaataaaag ccctcttcaa tgacggggcc gataaccatt
     9121 tttgcattag gatagagttg cttgacggca tgcccgacaa gatgggcgca ggaatggcgg
     9181 atgatttcga tgccttcctg atctttcgga gtaatgattt gaacagcaga attttcaaca
     9241 atcgggtcgc acacatcgac caatttgccg tttaccctgc ctgccaccgc cgccctcgcc
     9301 aaaccgacac cgatagacgc agcaatttga gccacggtaa cgggggattc gtattggcgg
     9361 actgagccgt ccggcaaggt aatgttcaac atcaaaaagc tccaaaacat taaaaaataa
     9421 cggcataaat gccgttatgg gaatttggta ggcacgattg gattcgaacc aacgaccccc
     9481 accatgtcaa ggtggtgctc taaccaactg agctacgtgc cttcaaagaa tttttgcatt
     9541 ttatcgggtc gttttaattt ttgcaagagg tatgtgcatt tctttatgca acattgtggc
     9601 aagatttggg gatgtcgtct gaaaaaccgt gggaattcat ttataatgcc gtcttttaaa
     9661 acatatttcg ccccctgtcg cccaactgac attcaatctt atgcttaaat ttaccttaca
     9721 caaaaaagac ggatacgccc gacgcggcac gctggagttg aaccacggca aaattgaaac
     9781 gccggtattt atgcctgtcg gtacttacgg ctcggttaag gcgatgaacc cgcaaaacct
     9841 gcacgacatc aaggcgcaaa ttattttggg caacacttat catttgtggc tgcgtccggg
     9901 cttggaggtc gtcgaacagt ttggagggct tcacggcttt atcggctggg acaaaccgat
     9961 tctgaccgac tcgggcggtt ttcaggtttt ttctttgtcg gatatgcgca agctgacgga
    10021 agaaggctgt acgttcaaaa gcccgatcaa cggcgacaag ctgtttttat cgcctgaaat
    10081 ttccatgaaa atccaaacgg ttttgaactc ggacatcgcg atgcagctgg acgaatgcac
    10141 gccgggcgag gcgacgcgcg aacaggcgcg aaaatcgttg caaatgagcc tgcgctgggc
    10201 ggaacggagc aaaaaagcct ttgaagattt gaaaaacccg aacgcactgt tcggcatcgt
    10261 gcaaggcgcg atgtatgaag atttgcgcga agagtcgctg aaaggcttgg aagagctcga
    10321 tttccccggc ttggccatcg gcggcttgtc cgttggcgaa cctaagcccg aaatgtaccg
    10381 catgttgcgc gccgtcggcc ctatcctgcc ggaacacaaa cctcattatt taatgggcgt
    10441 cggtacgcct gaagacttag tgtacggcgt ggcacacggc gtggatatgt tcgactgcgt
    10501 catgcctact cgcaatgcgc gtaacggctg gctgtttacc cgttttggcg atttgaagat
    10561 caaaaacgcc aaacacaagc tcgacaagcg tccgatagac gaaagctgca cctgctacgc
    10621 ctgccaaaac ttcagccgcg cctacctgca ccatctgcac cgcgccggcg aaatcttggg
    10681 cgcacagctc aacaccatcc acaacctgca tttctaccaa gtcattatgg cggaaatgcg
    10741 cgatgccata gaacaaggca aatttgccga ctggcaggcg cgattccacg aaaaccgtgc
    10801 tcgcggcaca gactgactcc cggcaatgcc gtctgaagcc gcttcagacg gcattttgcc
    10861 gctttaccct acccgcacta tttttcagaa accgatcatg cttccattct ttccagaacc
    10921 ctccctttcc tatacgcaac aaaaccgtac cgccgttttg ctgttgaacc tcggcacgcc
    10981 cgacgcgccg accgcgcagg cggtacgacc ttatctgaag tcctttctga ccgaccggcg
    11041 cgtcgtcgaa ctgcccaaat ggctgtggta tcccattctg catggtctgg tactgacgtt
    11101 gcgccccaaa aaaagcgcgc acgcctatga aaaaatctgg tttaaggaag gttcgcccct
    11161 agaagtttac acagcacgcc aagctgccgc gcttgccgaa cgtatgcccg acctcatcgt
    11221 ccgccacgcc atgacctacg gcaatccttc ggtggcagac gtattgtcgg aactgaaggc
    11281 gcaaggcgcg ggcagactgc tggtcatccc gatgtacccg caatatgccg cttccagcag
    11341 cggagcggca gtagataagg tgtgcgaaca attactgctg caacggaacc aaatgagcgt
    11401 ccgcaccgtt tcgcgctttt atgacgatac cggctacatt gatgcgatga aaaaccacat
    11461 cctccgatat tgggcggaac acggacgtgg gaaaaaactg atgttgagtt ttcacggcgt
    11521 accgcagaag cactacgacc tcggcgaccc ctatcccgac gagtgccgcc acaccgccaa
    11581 actgcttgcc gaagcactgg aactgaccga agaccaatat gtcgtatcgt tccaaagcca
    11641 attcggcagg gcaaaatggg ttacaccgag cacgcaggat ttgttcggca aactgcccaa
    11701 acagggggta accgagctgg acgtattctg cccgggcttt ttggcagact gtttggaaac
    11761 catggaagaa atcgccctga tggggcggga acagttttac gaagcaggcg gcaaaagcta
    11821 ccgctacatc ccctgcctca acgacaaccc cgactggata gacgcactcg tcgcacttgc
    11881 cgaagaaaac cttggcggct ggcgttgaaa ctgtttgacc catgtctaaa atgccgtctg
    11941 aacgccgtgt tcagacggca ttttcccgct actttagcgt atgatggcat ctatgccgga
    12001 caacaacatc aagtgcaacc ttactaccga ttcggcaaaa aaatctttca cccgctacgc
    12061 aaaatcaaca acaaggtttc aaacattaag gaaacaccaa tgaacacaac ccgactgccg
    12121 accgccctcg tcttgggctg cctctgcgcc gccgcttctg ccgccgacaa cagcatcatg
    12181 acaaaagggc aaaaagtgta cgaatccaac tgcgtcgcct gccacggcaa aaagggcgaa
    12241 ggccgcggaa ccatgtttcc gccgctctac cgctccgact tcatcatgaa aaaaccgcag
    12301 gtgctgctgc acagcatggt caaaggcatc aacggtacaa tcaaagtcaa cggcaaaacc
    12361 tacaacggat tcatgcccgc aaccgccatc agcgatgcgg acattgccgc cgtcgccact
    12421 tatatcatga acgcctttga caacggcggc ggaagcgtta ccgaaaaaga cgtaaaacag
    12481 gcaaaaggca aaaaaaacta aacagacaaa atgccgtctg aaaccggcaa tccggcttca
    12541 gacggcattc aaatcaaacc tttctaacgc ggtactttca gccccataga cccgatattg
    12601 ccgtccccgt caacctcctt cgcggtcagc ataaagccgc cgatgtcaat acggccgcac
    12661 tcgaccggct cctgactgtc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
    12721 tcgcccgctt ccagcctcaa accgtaagca agtgccaaat cacccgaacg tgctgccggc
    12781 aaaacgacaa actcaccgaa gaaatcaaaa tactcacgga cgctaatacc cgtctcggta
    12841 aagtatttcg ccattttata gtgaa
//
LOCUS       DAXHTR010000053        12714 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.053, whole genome shotgun sequence.
ACCESSION   DAXHTR010000053 DAXHTR010000000
VERSION     DAXHTR010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12714
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.053"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..1722
                     /locus_tag="ACFNML_001011"
     CDS             <1..1722
                     /locus_tag="ACFNML_001011"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006306 - obsolete DNA methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6392416.1"
                     /translation="LYTKLTRKRQEIFFNQTLAFDEIDRLFDAKAFSKFSRYTADGKQ
                     AVGEIKRHSDGTPAENLIIKGNNLIALHSLAKQFKGKVKLIYIDPPYYFVKKKQQDSF
                     GYNTNFKLSTWLTFMKNRLSIAKELLTDDGIIVISIDDDGNAYLKILLDEIFGFENFI
                     GNLPTIMNLKGNNDEYAFAGTHEYTLVFAKNKDKSTFYEFPIDEDNFLEKWEEDEIGF
                     YKKGAPMRATGTEDKREDRPEMFYPFLVKNNTVSTITDEEFSQIYNKDLEVFNDDFIQ
                     KLKEKYENLGYNFILPIADKQWGRWRWGYSIKNKARLQTDIIVSQSKNGISLYKKQRP
                     ELDDLPTKKPKTIFYKPEYSSGNGTEQMKNLFGEKAFKNPKPEELIQDFITITTNESD
                     IVLDYHLGSGTTAAVAHKMNRQYIGIEQMDYIETLAVERLKKVIDGEQGGISKAVNWQ
                     GGGEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSVNEFSQIIQE
                     PEFQSLALDEQKQMVLEMLDLNQMYVSLSEMDDEQFAGCLNDDDKALSREFYQSAKNQ
                     AEKKDGE"
     gene            1712..4492
                     /locus_tag="ACFNML_001012"
     CDS             1712..4492
                     /locus_tag="ACFNML_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFC6392417.1"
                     /translation="MANNKTLFDWVEDRKSTLEEIEQTDFFALPEFVERNLKYPFFEW
                     QKSALENFVIFDRASKLKDFPDIKNRPTHLLFNMATGAGKTMMMAALILYYFEKGYRH
                     FLFFVNQNNIVDKTENNFIDPAHSKFLFTEKILQGDQVIPIHKVETFSPHSDGIEIKF
                     TSIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKA
                     NAGDAEFERKGWEHMVLELLLNKNGNPGQNVLLEFTATPPEKADVQQKYADKIITKFG
                     LKEFLQKGYTKEINLVSSTLSKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKT
                     VDESKADYLAFLNWVENVQADDFSFLKTFSMSLNDSDNANEQGKTRTEQALKFMQENG
                     FEFAHLANWIKQNYQKHNIIITNSETNKSKTEKTDSETEKLLNNLEAVDNPIRAIFTV
                     GRLTEGWDVLNLFDIVRLYEGQNGGGSNKKSCKTANTTVSEKQLIGRGVRYFPFSFED
                     KQPNKRKFDNDMQHELRILEELFYYTHDEQSRYITELKNELRKDGYLPEKDDDKVLAT
                     FKLKSEFADNENFKNLLIWANKKIPNPNAKSNNADSLKANPQTLSFQIHGNQLLQETQ
                     FTADKNDEKVKQLGIQNNFSQTIKMSEMERHIFNKALHIKGKNSQSLFHFNRLQSKLN
                     IQNRNELQNNLLKDWQIEFLGLEQDKQVRPDDKLAGCLKILEMVEKHLNESDKPFIGT
                     KEFTPKTLWEIFGTPKQKWVKKDDIKTAIAMQNDWYVMDNFAGTGLEEALIQFISARL
                     GDLKSQYDIHLIRNEEVFKLNNFANGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFI
                     EPKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTES
                     FPLGAASLEKMK"
     gene            complement(4636..5808)
                     /locus_tag="ACFNML_001013"
     CDS             complement(4636..5808)
                     /locus_tag="ACFNML_001013"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HFC6392418.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALI
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            6099..6545
                     /gene="iscR"
                     /locus_tag="ACFNML_001014"
     CDS             6099..6545
                     /gene="iscR"
                     /locus_tag="ACFNML_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFC6392419.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            6574..7788
                     /locus_tag="ACFNML_001015"
     CDS             6574..7788
                     /locus_tag="ACFNML_001015"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HFC6392420.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHSFG
                     WEAEEAVEKARADIAALINADSKEIVFTSGATESNNLAIKGAAHFYKSKGNHLITVKT
                     EHKAVLDTMRELERQGYEVTYLDVQENGLVDLDVLKAAIREDTILVSVMWVNNEIGVV
                     QDIPAIGEICRERKIIFHVDAAQACGKVPVDVEAAKVDLLSMSGHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            7855..7983
                     /locus_tag="ACFNML_001016"
     CDS             7855..7983
                     /locus_tag="ACFNML_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392421.1"
                     /translation="MNEQDLDLDNLDNLLDDFDGVTVEGGVDSENDDGCEGGACKI"
     gene            8049..8435
                     /gene="iscU"
                     /locus_tag="ACFNML_001017"
     CDS             8049..8435
                     /gene="iscU"
                     /locus_tag="ACFNML_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFC6392422.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            8522..8842
                     /gene="iscA"
                     /locus_tag="ACFNML_001018"
     CDS             8522..8842
                     /gene="iscA"
                     /locus_tag="ACFNML_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFC6392423.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(8802..9023)
                     /locus_tag="ACFNML_001019"
     CDS             complement(8802..9023)
                     /locus_tag="ACFNML_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HFC6392424.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            9105..9605
                     /gene="hscB"
                     /locus_tag="ACFNML_001020"
     CDS             9105..9605
                     /gene="hscB"
                     /locus_tag="ACFNML_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001671 - ATPase activator activity
                     [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HFC6392425.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKKSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            9703..12453
                     /gene="gyrA"
                     /locus_tag="ACFNML_001021"
     CDS             9703..12453
                     /gene="gyrA"
                     /locus_tag="ACFNML_001021"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HFC6392426.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDTAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPTLLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVMRGKTHIEPIGRNGEREAIVIDEIPYQ
                     VNKAKLVEKIGDLVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAAEAKDKLLAHPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGMVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            12610..12714
                     /locus_tag="ACFNML_001022"
                     /pseudo
     CDS             12610..12714
                     /locus_tag="ACFNML_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         3895 a         3116 c         2773 g         2930 t
ORIGIN      
        1 ttatacacta aattaacccg aaaaagacaa gaaatctttt ttaatcaaac gcttgctttt
       61 gatgaaatcg atcggctttt tgacgcaaaa gcattctcaa aattctctcg ctataccgca
      121 gacggcaaac aagcggttgg cgaaatcaaa cgtcattcag acggcacacc cgccgaaaat
      181 ctcattatca aaggcaataa tctgatagcc ctacattcgc ttgccaaaca gtttaaaggc
      241 aaagtgaagt tgatttatat tgatccgcct tattattttg ttaaaaagaa acaacaagat
      301 agttttggtt ataacactaa ctttaaatta tctacttggc ttacatttat gaaaaatcgt
      361 ttatcaattg ccaaagaatt attaactgat gatgggatta ttgttatttc cattgatgat
      421 gacggaaatg cttatttaaa gattttgtta gatgaaattt ttggatttga aaattttatc
      481 ggaaatttac ccactattat gaatttaaaa ggtaataatg atgaatatgc ttttgcaggc
      541 actcacgaat acacactagt ctttgctaaa aataaagata aatctacttt ttatgaattt
      601 ccaatagatg aagataattt tttagagaaa tgggaagaag atgaaattgg gttttataaa
      661 aaaggagctc caatgcgagc aacaggaacc gaagataaaa gagaagatag acctgaaatg
      721 ttctatcctt ttttggtaaa aaataacaca gttagtacaa ttacagatga agaattttct
      781 caaatctata ataaagattt agaagtattc aatgatgatt ttattcaaaa actaaaagag
      841 aaatacgaaa atcttggata caattttatt ttacctattg ctgataaaca atgggggcgt
      901 tggcgttggg ggtattcaat aaagaataag gcaaggctgc agacggatat tattgttagc
      961 caatctaaaa atggaatttc cttgtataaa aaacaaagac ctgaattaga tgatttacct
     1021 acaaaaaaac ctaaaactat tttttataaa cctgaataca gtagtggtaa tggaactgaa
     1081 cagatgaaaa atctttttgg tgaaaaggca tttaaaaatc ctaaaccaga agaacttatc
     1141 caagatttta ttaccatcac aaccaacgaa tccgacatcg tcttagacta ccatcttggt
     1201 agtggcacaa ccgccgccgt tgcccacaaa atgaaccgac aatatatcgg tattgaacaa
     1261 atggattata ttgaaacgct tgccgttgaa cgcttgaaaa aagtgattga tggcgagcaa
     1321 ggcggtattt ccaaagccgt gaattggcaa ggtggcggcg agtttgtcta tgccgaactt
     1381 gctccattta acgaaaccgc aaaacaacaa attttggctt gcgaagattc agacggcatc
     1441 aaaacgctgt ttgaaggttt atgcgaacgc tatttcttga aatacaacgt cagcgtaaat
     1501 gaatttagtc aaatcattca agagcctgaa tttcaatcct tggcattaga cgaacaaaaa
     1561 caaatggtgc ttgaaatgtt ggatttaaat caaatgtatg tttcattatc cgaaatggat
     1621 gatgagcaat ttgcaggttg cttaaatgat gacgataaag cgttaagccg tgaattctat
     1681 caatcagcaa aaaatcaagc ggagaaaaaa gatggcgaat aataaaacgt tatttgattg
     1741 ggtggaagac cgcaaatcaa cgctggaaga aatagaacag acagattttt tcgcgctgcc
     1801 tgaatttgta gaacgcaatt taaaatatcc gttttttgaa tggcaaaaat cggctttaga
     1861 aaactttgtg atttttgacc gcgcttcaaa attaaaggat ttccctgaca tcaaaaaccg
     1921 ccctacccat ttgctgttca atatggcaac aggtgctggc aaaacgatga tgatggcggc
     1981 gttgattttg tattattttg aaaaaggtta tcggcatttt ttgtttttcg tcaatcaaaa
     2041 taatatcgtg gataaaacgg aaaataattt tattgacccg gcgcactcaa aatttttatt
     2101 taccgagaag attttgcaag gcgaccaagt tattcccatt cacaaagtag aaacgtttag
     2161 cccacattca gacggcattg aaattaaatt taccagtatt caaaaactgt ataacgatat
     2221 ccacaccgaa cgggaaaacc aaaccacatt ggcagatttg cacgaattga accttgtgat
     2281 gctgggcgat gaagcgcacc atttaaacgc acaaaccaaa ggcaaaaaac aaggcgagtt
     2341 ggatttggaa gtggaattaa aagccaatgc aggcgatgcc gaatttgaac gcaaaggctg
     2401 ggagcatatg gttttggaat tgttgctcaa taaaaatggc aatcccggcc aaaatgtgct
     2461 gttggaattt accgccacgc cacctgaaaa agctgacgtg caacaaaaat acgctgataa
     2521 aatcatcaca aaatttggtt taaaagaatt tttgcaaaaa ggttatacca aagaaatcaa
     2581 tttggtatcc agtacactga gcaagaaaga gcgggtgttg cacgctttat tgtttgcttg
     2641 gtatcgccat caaatcgctt tgaaatatgg cattgccaat ttcaagcctg tgatgttgtt
     2701 tagaagtaag acggttgatg aatcaaaagc ggattatttg gcatttttaa attgggtaga
     2761 aaatgtgcaa gcagatgatt tttcgttttt aaagacattt tcaatgagct taaacgatag
     2821 cgataacgcc aacgaacaag gcaaaacccg caccgaacaa gccctaaaat ttatgcagga
     2881 aaatggcttt gagtttgcac atttagcaaa ttggataaaa caaaattatc aaaaacacaa
     2941 tatcattatt accaactccg aaaccaacaa aagcaaaacc gaaaaaaccg acagcgaaac
     3001 ggaaaaattg ctaaacaatc tggaagcggt tgataatccg attcgtgcta tttttacggt
     3061 gggtagatta accgaaggtt gggacgtttt aaatttgttt gatattgtgc gtttgtatga
     3121 agggcaaaac ggcggcggtt caaataaaaa atcatgcaaa acggcaaata caacggtatc
     3181 agaaaaacag ttgattggtc gaggcgtgcg ttattttcca ttttcgtttg aagataaaca
     3241 gccgaataag cgtaaatttg acaacgacat gcaacacgaa ttgcgtattt tggaagaatt
     3301 gttttattac acgcacgatg agcaatcccg ctatattaca gaactgaaaa acgagttacg
     3361 aaaagacggt tatttgcctg aaaaagacga tgataaggta ttggcaacat ttaaactcaa
     3421 atctgaattt gcagataatg aaaattttaa aaacttattg atttgggcga ataaaaaaat
     3481 ccccaatccc aatgctaaaa gtaataatgc agacagccta aaagccaatc cgcaaacact
     3541 ttcattccaa attcacggca atcaactgtt gcaggaaacg caatttacag ccgataaaaa
     3601 tgatgaaaag gtcaagcaac ttggcataca aaataatttt tctcaaacca taaaaatgag
     3661 tgaaatggaa cggcacatct tcaataaagc attacatatc aaagggaaaa atagtcaatc
     3721 tttattccat ttcaaccgtt tgcaaagcaa actcaacatt caaaatcgca atgaattgca
     3781 aaataacttg ttgaaagact ggcaaattga atttttagga ttagagcaag ataaacaggt
     3841 tcgcccggat gataaacttg caggctgcct gaaaatcttg gaaatggttg aaaagcattt
     3901 gaatgaaagt gataagccat ttatcggtac aaaagaattt acgcctaaaa cattatggga
     3961 aattttcggc acgccaaaac aaaaatgggt caaaaaagat gatataaaaa ctgctattgc
     4021 catgcaaaat gattggtatg tgatggataa ttttgccgga acgggtttgg aagaagcgtt
     4081 aattcaattt atttcagcgc gtttgggcga tttgaaatct caatatgaca ttcatttaat
     4141 ccgtaacgaa gaagtgttta aattgaataa ctttgccaat ggcgaaggat ttatgcccga
     4201 ctttatttta ttgctgaaag ataaacaaaa atcttcttcc aatggcgtga atgacttttt
     4261 gcattaccaa attttcattg agcccaaagg tgagcattta gtggaaactg atggatggaa
     4321 agaagaattt ttagaagcca tcacagtgga atatgggatg gataagatcc tgcaaaaaga
     4381 tacaccgcat tatcgtttga tcggtttacc gttttttact gatcatcaga aaaatggaca
     4441 atttacggag tcattccctt taggtgcggc atcgcttgaa aaaatgaagt agtgcattgc
     4501 aggcagcccc gtttgacaaa acttccttta caaaagggcg ttttgtcaga tatttaatca
     4561 acacattatt aaaatacagc caaattttaa tgccgtctga accctgtgtt cagacggcat
     4621 cgtatttttc agtatctaaa ccgtttccct accccaatcc ttacccctca aaatcgaggc
     4681 atcgacatct tgaatatcgc ggtgtcccgt aaacgccata gatatatcca tttctttata
     4741 caggatttcc agcgcacggg ttacgccttc ttcgccatac gcacccaagc catacagaaa
     4801 cgcccgacct atcattgtac ctttcgcacc caaagcccac gccttcaaaa tatcctgacc
     4861 gctgcggatg ccgctgtcca tccaaacttc gatgtcgctg ccgactgcac taacgatgtc
     4921 gggcaaggct ttgatggcag acacggtatc gtcgagctgt cggccgccgt ggttggaaac
     4981 aatcaatgcg tccgcgccgc ttttcgctgc tttttccgcg tcttcgggtt ccataatgcc
     5041 tttgataatc agcttgccgc cccacaaatc tttaatgcgc gccacatcgt cccagctcag
     5101 gcgcgggtcg aattgttcgg aagtccatga agacagcgaa gacaaatcgc cgacgttctt
     5161 cgcgtgtccg acgatattgc ggaacgtgcg gcgttccgtg ttcagcattt tcatgcacca
     5221 ttccggtttg gtcgccagat tgattaaatt ggcgatggtc ggtttcggcg gcgcggacag
     5281 gccgtttttg atgtctttgt ggcgttgacc caaaacctgc aaatcggcgg tcaataccaa
     5341 tgccgaacat ttggcatcct tcgcgcgctt aatcaggttt tccataaact cgcggtcgcg
     5401 catcacataa agctgaaacc aaaatggtgc ggaagtgttc tcggcaacgt cttcaatcga
     5461 gcagatagac atcgtggaca gcgtaaacgg aatgccgaac ttctccgccg cccgcgccgc
     5521 caaaatttca ccgtcggcgt gtgccatacc cgtgaaaccc gtcggcgcaa tcgccaccgg
     5581 cattttcaca tcctgcccga tcattttggt ttccaggctt cggccttcca tattgaccaa
     5641 taccttttgt cggaagcgga tgtctttgaa atccgaagtg ttttcacggt aggtagtttc
     5701 tgtccacgaa cccgaatcga tgtaatcgta aaacatacgc ggcattttgc gcttggcaac
     5761 gcggcgcaag tcttcgatgc aggtcatttt gctcaaatca cgtttcattt gtcgccccct
     5821 gaatacctga ataactttat atgaaatcga taatgtatat caatattgat tataaggcaa
     5881 atcatttcaa catttgccgc atccgccgca gctccctact ttaagcgaca taaggtttaa
     5941 aattcaaaaa taacaaatta aaatcaaaat attaaaaatc aatcaatcta tcgatttaaa
     6001 cagccaatca cacaatccgc ccccatactt gactgaaaca ctcagatatt ggacaattcc
     6061 acccactaat aaaaaaaccg acatgggcaa ccaccaccat gagactgacc accaaagggc
     6121 gtttcgccgt taccgctatg ctggatttgg cgatgaacgc gcaaaccggc gccgtcaaac
     6181 tcagtgccat cagcgaacgc caaaacatat ccctctccta tctcgagcaa ttgttcggca
     6241 aactccgccg cgccggactt gttgaaagcc tgcgcgggcc cggcggcggc tacatcctcg
     6301 ccgcaccggc ggcacgcatc aacatcgccc aaatcatcgc cgccgccgaa gaccggctgg
     6361 acgcaaccca atgcggcagc aaagccaact gccaccacgg cgcgccctgc ctgacgcacg
     6421 atctttggga gaatttaaac aaaaccatca acgactacct cggcagcgtt accctgcaaa
     6481 gcatcatcga acagaaaaac aacggcgacg gcagccgcgt cgtccaattt acacacatcc
     6541 attaaataac acccgaaaaa gaaagagcaa accatgaccg tcaaaacccc cgtttacctc
     6601 gactacgccg ccaccacccc cgttgacaaa cgcgttgccg aaaaaatgat tccctatctg
     6661 accgaaacct tcggcaaccc agcctccaac agccacagct tcggctggga agcagaagaa
     6721 gctgtagaaa aagcacgtgc agacattgcc gccctgatta acgccgactc taaagaaatc
     6781 gttttcacca gcggcgcaac cgagtccaac aacctcgcta tcaaaggcgc ggcgcacttc
     6841 tacaaatcta aaggtaatca cctcatcact gtaaaaaccg aacacaaagc cgtactcgac
     6901 accatgcgcg aactcgaacg ccaaggttac gaagtaactt atctggacgt acaagaaaac
     6961 ggtttggttg atttagacgt actgaaagcc gccatccgcg aagacaccat cctcgtttcc
     7021 gtaatgtggg taaacaacga aatcggcgtg gttcaagata ttcctgccat cggcgaaatc
     7081 tgccgcgaac gcaaaatcat tttccacgtt gacgcagcac aagcatgcgg caaagtgcct
     7141 gttgatgttg aagccgcaaa agttgatttg ctgtctatgt ccggccacaa agtatacggc
     7201 cctaaaggca tcggcgcgct gtacgtccgc cgcaaacccc gcgtccgcct cgaagcccaa
     7261 atgcacggcg gcggccacga acgcggcttc cgcagcggca cattgccgac ccaccaaatc
     7321 gtcggtatgg gcgaagcctt ccgcatcgcc aaagaagaac tcgaacaaga tatggcgcac
     7381 taccgcaaac tgcgcgacat cttcctcaaa ggcatcgaag gcatcgaaga agtctatgtc
     7441 aacggcgacc tcgaacaccg cgccccgaac aacctgaacg tcagcttcaa cttcgtcgaa
     7501 ggcgaaagcc tgattatggc ggtgaaagaa ctcgccgtat ccagcggctc cgcctgtact
     7561 tctgccagtc tcgaacccag ctacgtcctg cgcgcgctcg gccgcaacga tgaactggcg
     7621 cactcatccc tgcgcatcac cttcggtcgc atgaccactg aagaagaagt gcaatttgcc
     7681 gccgaactga ttaaatccaa aatcggcaaa ctgcgcgaac tgtcgccgtt gtgggaaatg
     7741 ttcaaagacg ggattgattt gaactcgatt gaatgggcgg cgcattaaag cgtaccaaca
     7801 tgccatccga accttcagac ggcattccaa aaacaaagca atcaagagaa aaatatgaac
     7861 gaacaagatt tagatttgga caacctcgac aacctgcttg acgatttcga cggcgttacc
     7921 gtggaaggcg gcgtcgattc ggaaaacgac gacggctgtg aaggcggagc gtgcaaaatc
     7981 taactcaggc cgtctgaaaa ccggcaaaca aaccacttaa atcatcaaaa cattaaggaa
     8041 accacatcat ggcatacagc gataaagtaa tcgaccacta cgaaaatccc cgcaacgtcg
     8101 gtactttcga caaaaacgac gaatctgtcg gcaccggcat ggtcggcgca ccggcctgcg
     8161 gcgacgtgat gcgcctgcaa atcaaagtga acgatgaagg catcatcgaa gatgcgaaat
     8221 tcaaaactta cggctgcggt tccgccatcg cttcgtccag cctgattacc gagtgggtta
     8281 aaggcaaaag cctggatgac gcgctggcaa tcaaaaacag cgaaatcgcc gaagagttgg
     8341 aattgccgcc ggtaaaaatc cactgctcca tcttggctga agatgcggta aaagcggccg
     8401 ttgccgacta ccgcaaacgt caggaaaaca gataaagccc ttcagacggc atcgtcccgc
     8461 aatgccgtcc gaaccgcccg ccgcttcggg ttcgtccgag gcggtacaac aaggaagaaa
     8521 tatgattacc cttaccgaga atgccgcaaa acacatcaat gactatctcg ccaaacgcgg
     8581 caaaggcttg ggcgtacgct tgggtgtgaa aaccagcggc tgctcgggga tggcgtacaa
     8641 ccttgaattt gtcgatgaag ccgatggcga cgacctgatt ttcgaaggac acggcgcacg
     8701 catttatatc gacccgaaaa gcctggttta tctggacggc acacaagtcg attacaccaa
     8761 agaaggtttg caggaaggtt tcaaatttga aaaccccaat gtcaaagact cctgcggctg
     8821 cggcgaaagc ttccatgttt aagacataaa aatggcggga ccgtatcaaa accgtcccgc
     8881 cattttttcg cttcctgcct gttgtagctg cctttgcctt ttcttttccg ttccaccttg
     8941 tgtcggaaca aatcagattt cactaaggct tttaaagcat tgtcgcgtat tttgccttta
     9001 ttgtgctgca ctttgccgcc catattcagt cctttcgttt aagaagcggc agattataag
     9061 gcaaaaacag ttttctgcca aaatcttaca tttatcatcc tactatgtcc caatatttca
     9121 ccctcttccg gattgaaccc gctttcgata tcgacaccga aaacttggaa caaacctacc
     9181 gcgccttggc cgcccgtttc catcccgata aattcgcttc agcttccgcc tttgagcaaa
     9241 agcaggcagt gatgatgtct tccaccatca acgatgccta ccgcaccttg aaaaacccca
     9301 tcgaccgcgc cgcctacctg ctgaaaaaat cgggcatcga tgccgacgcg ccggagcata
     9361 ccgctttcgc ccccgaattc cttatgcagc aaatggaatg gcgcgaaacg ctgatggagg
     9421 cacgggcagg caacgacctt gaatccttga aaaatctcga caacgaaatc cgcgacgagc
     9481 aagaaaaact gttctgcggt ctgaaacagt cgtttgcccg acaagattac gacacagccg
     9541 cacaacaagt ccgccaaggc aggtttctcg acaaactccg caacgaaatt tcctcggcat
     9601 tataatccgc accgtgtttc agacggcgta accgccgcac cgttccgcgt caaaatatgc
     9661 taaaataagc aacaattttt tgccatacga aacattgaaa ccatgaccga cgcaaccatc
     9721 cgccacgacc acaaattcgc cctcgaaacc ctgccggtaa gccttgaaga cgaaatgcgc
     9781 aaaagctatc tcgactacgc catgagcgtc attgtcgggc gcgcgctgcc ggacgttcgc
     9841 gacggtctca agccggtaca ccgccgcgta ctgtacgcga tgcacgagct gaaaaacaac
     9901 tggaatgccg cctacaaaaa atcggcgcgc attgtcggcg acgtcatcgg taaataccac
     9961 ccccacggcg ataccgccgt atacgacacc atcgtccgta tggcgcaaaa tttcgctatg
    10021 cgttatgtgc tgatagacgg acagggcaac ttcggatcgg tggacgggct tgccgccgca
    10081 gccatgcgct acaccgaaat ccgcatggcg aaaatttccc acgaaatgct ggcagacatt
    10141 gaggaagaaa ccgtcaattt cggcccgaac tacgacggta gcgaacacga gccgcttgta
    10201 ctgccgaccc gtttccccac actgctcgtc aacggctcgt ccggcatcgc cgtcggcatg
    10261 gcgaccaata tcccgccgca caacctttcc gataccgtca atgcctgcct gcgcctgctc
    10321 gatgcacccg acaccgaaat cgacgaactg atcgacatta tccaagcccc cgacttcccg
    10381 accggggcaa ccatctacgg cttgagcggc gtgcgcgaag gctataaaac aggccgcggc
    10441 cgcgtcgtta tgcgcggtaa gacccatatc gaacccatag gcagaaacgg cgaacgcgaa
    10501 gccatcgtta tcgacgaaat cccctatcag gtcaacaaag ccaagctggt cgagaaaatc
    10561 ggcgatttgg ttcgagaaaa aacactggaa ggcatttccg agctccgcga cgaatccgac
    10621 aaatccggta tgcgcgtcgt tatcgagctg aaacgcaacg aaaatgccga agtcgtctta
    10681 aaccaactct acaaactgac tccgctgcaa gacagtttcg gcatcaatat ggtggttttg
    10741 gtcgacggac aaccgcgcct gttgaacctg aaacagattc tctccgaatt cctgcgccac
    10801 cgccgcgaag tcgttacccg acgtacgctt ttccggctga agaaggcacg ccatgaaggg
    10861 catatcgccg aaggcaaagc ggtcgcactg tccaatatcg atgaaatcat caagctcatc
    10921 aaagaatcgc ccaacgcagc cgaggccaaa gacaaactgc ttgcgcaccc ttggcgcagc
    10981 agcctcgttg aagaaatgct gacgcgttcc ggtctggatt tggaaatgat gcgtccggaa
    11041 ggattggctg caaacatcgg cttgaaagag caaggttatt acctgagcga gattcaggca
    11101 gatgctattt tacgcatgag cctgcgaaac ctgaccggcc tcgatcaaga agaaattgtc
    11161 gaaagctaca aaaacctgat gggtaaaatc atcgactttg tggatatcct ctccaaaccc
    11221 gaacgcatta cccaaatcat ccgcgacgaa ctggaagaaa tcaaaaccaa ctatggcgac
    11281 gaacgccgca gcgaaatcaa cccgttcggt ggcgacattg ccgatgaaga cctgattccg
    11341 caacgcgaaa tggtcgttac cctgacacat ggcggctata tcaaaaccca gccgaccacc
    11401 gactatcagg cgcagcgtcg cggcgggcgc ggcaaacagg cggctgccac caaagacgaa
    11461 gactttatcg aaaccctgtt tgttgccaac acgcatgatt atttgatgtg ctttaccaat
    11521 ttgggcaagt gtcattggat taaggtttac aaactgcccg aaggcggacg caacagccgc
    11581 ggccgtccga ttaacaacgt catccagttg gaagaaggcg aaaaagtcag cgcgattctg
    11641 gcagtacgcg agttccccga agaccaatac gtcttcttcg ccaccgcgca gggaatggtg
    11701 aaaaaagtcc aactttccgc ctttaaaaac gtccgcgccc aaggcattaa agccatcgcg
    11761 ctcaaagaag gcgactacct cgtcggcgct gcgcaaacag gcggtgcgga cgacatcatg
    11821 ctgttctcca acttaggtaa agccatccgc ttcaacgaat actgggaaaa atccggcaac
    11881 gacgaagcgg aagatgccga catcgaaacc gaaatttcag acggcatcga agatgaaacc
    11941 gccgacagcg aaaacgcact gccgagcggc aaacacggtg ttcgcccgtc cggtcgcggc
    12001 agcggcggtt tgcgcggtat gcgcctgcct gccgacggca aaatcgtcag cctgattacc
    12061 ttcgcccctg aaaccgaaga aagcggtttg caagttttaa ccgccaccgc caacggatac
    12121 ggaaaacgca ccccgattgc cgattacagc cgcaaaaaca aaggcgggca aggcaatatt
    12181 gccattaaca ccggcgagcg aaacggcgat ttggtcgccg caaccttggt cggcgaaacc
    12241 gacgatttga tgctgattac cagcggcggc gtacttatcc gcaccaaagt cgaacaaatc
    12301 cgcgaaaccg gccgcgccgc agcaggcgtg aaactgatta acttggacga aggcgaaacc
    12361 ttggtatcgc tggaacgtgt tgccgaagac gaatccgaac tctccgacgc ttctgtaatt
    12421 tccaatgtaa ccgaaccgga agtcgagaac tgaaaatcat ctcccgatgc cgtctgaaga
    12481 ttcagacggc atttatttta tccctcatcc gtcatccagc ttctcacaat atagcggatt
    12541 atagtcaatt aaaaacaagg ggctgtccta gataactagg gaaattcaaa ttaagttaga
    12601 gttgccccta tgagaaaaag tcgtctaagc cggtataaac aaataaactc attgaactgt
    12661 ttgtcgcagg tgtaactgca agaacgacag cagagttagt aggcgttaat aaaa
//
LOCUS       DAXHTR010000054        12640 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.054, whole genome shotgun sequence.
ACCESSION   DAXHTR010000054 DAXHTR010000000
VERSION     DAXHTR010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12640
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.054"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(498..1790)
                     /locus_tag="ACFNML_001023"
     CDS             complement(498..1790)
                     /locus_tag="ACFNML_001023"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HFC6392427.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIALTERTERVRPQRV
                     EQNLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1867..2835
                     /locus_tag="ACFNML_001024"
     CDS             1867..2835
                     /locus_tag="ACFNML_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392428.1"
                     /translation="MKLTLALPSLNLDEDEIRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSETVALATPVWQKMGMHQANVLTAEYLDVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGADDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWGGTNGGTQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYAAILQQWEERWFAP
                     LYEAVRIGKIKRLDIATDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(3036..4502)
                     /locus_tag="ACFNML_001025"
     CDS             complement(3036..4502)
                     /locus_tag="ACFNML_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6392429.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4744..6891)
                     /locus_tag="ACFNML_001026"
                     /pseudo
     CDS             complement(4744..6891)
                     /locus_tag="ACFNML_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_180374912.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(8364..8609)
                     /locus_tag="ACFNML_001027"
     CDS             complement(8364..8609)
                     /locus_tag="ACFNML_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392430.1"
                     /translation="MLKSIELNSHVRNRLAAYLKGRGMDFQTAMQEEEGNKEIAAIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            8726..9151
                     /locus_tag="ACFNML_001028"
     CDS             8726..9151
                     /locus_tag="ACFNML_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HFC6392431.1"
                     /translation="MYSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYVR
                     LSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLILLAYQLYCGVL
                     LRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            9163..9639
                     /locus_tag="ACFNML_001029"
     CDS             9163..9639
                     /locus_tag="ACFNML_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HFC6392432.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGENAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(9989..10477)
                     /locus_tag="ACFNML_001030"
     CDS             complement(9989..10477)
                     /locus_tag="ACFNML_001030"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HFC6392433.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(10545..11600)
                     /gene="aroG"
                     /locus_tag="ACFNML_001031"
     CDS             complement(10545..11600)
                     /gene="aroG"
                     /locus_tag="ACFNML_001031"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFC6392434.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            12267..12524
                     /locus_tag="ACFNML_001032"
     CDS             12267..12524
                     /locus_tag="ACFNML_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392435.1"
                     /translation="MTTQMCASRDVSKSAKGWSVGIGLNVGKQLTDSVGLEFDPYYRH
                     KTICKPREIVLDGDKTKMGRSKSNEYGFRVAATFYSQLKSK"
     gene            complement(12506..>12640)
                     /locus_tag="ACFNML_001033"
     CDS             complement(12506..>12640)
                     /locus_tag="ACFNML_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC6392436.1"
                     /translation="PLAPYSPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN
                     "
BASE COUNT         2883 a         3479 c         3125 g         3153 t
ORIGIN      
        1 tgcaaacact gccagaacgg ataaatccgg attcccgccc acttcgtcat tcccgacgcg
       61 cacgccgctt tcccgcccac ttcgtcattc ccgcgaaagc gggaatccgg tgcgttgggt
      121 ttcagtcatt tccgataaac tcctgccgca ttgtatttct agattcccgc tttcgcggga
      181 atgacggcgg atgggtttcc gtaccgtggt gtccggattc ccgacggtac gggaatgacg
      241 gcagtgcttc cttaacttag ttttatttta atcgactgta tggtggatta acaaaaatca
      301 ggacaaagcg acgaagccgc agacagtaca aatagtacga aaccgattca cttggtgctt
      361 cagcatctta gagaatcgtt ctctttgagc taaggcgagg caacgacgta ctggtttttg
      421 ttaatccact ataaatgccg tctgaaaacc tgtttgcagg ttttcagacg gcattgtttt
      481 cgtcgccggt tcaaacttca atccgattgc gacacggtaa ccggtatgcc gcgcaagcgc
      541 gcaagcagcg cgtccgcctt ctgtttctgc gcggcaaacg ccgccttgtc cgcctgcgtc
      601 aattcgggtg tcggcaatgc gactgaaaca ggattaacgg gctgcccgtt gatgcgcgcc
      661 tcgtaatgca ggtgcggccc ggtcgaacgc ccggtcgaac cgacaaaacc gatgacctcg
      721 ccgccgcgca cattgccttg tgcctgcgaa aacgcgctca agtgcgcgta cagcgtttcc
      781 acgccgttgg cgtggcgtat catcaccgcg ttgccgtatc cgcccttccg gcctttaaag
      841 gtaatcacgc cgtcggcgga agccctgacc ggcgttccct gcggtgcggc ataatcgatg
      901 cccgtgtgca gccgccatgt gtgcaggatg gggtgcatac ggtagccgaa cggcgaagaa
      961 atgcgcgtat agaccagcgg ctcgatgttg aagccgcctt tttcctgcaa caccctgccg
     1021 tcttcatcat aataattgcc gccccctccg ccttccttgt ccgaacggta atagaatgcc
     1081 tgatgccttg tgccgccctt aacgacttca gccgccaaaa tatcgcccgc cgccacctgc
     1141 tgcccgtgga aatacaggct gtcgtacatc aggcgcacgg catcgccttc cttcaaaccg
     1201 tcaaggctga agcggccggc gaaaatcccg cttaaggatt cgcggatttc gacgggcact
     1261 tccgcccgcg ccagcgaacc gcgcgccgac gttttgacca cgaccgaacg cagcgtcggc
     1321 aaaaccttca tatccgcctc agaagccgac cgccgccata tgccgccttt cttttccaaa
     1381 gcgaccagat tgcgctcgcc gtcttcgtcg gtaaaaaact gtacttcgcg cgcgccgccg
     1441 tcgccgccga ccaaaacatg aaccgactgg tcggcacgca aatgccgcaa atcggcttcg
     1501 ccgccatatt tttccgtgat tcgggcaatc tcgtcccgcg ccatacccga acgcgccagc
     1561 acgtccgcca gcgagtcgcc cggctgcacc gcctcctgca cccaatatgc cgtctgaacg
     1621 ccgctgccgc cccaagacag cggcggcaga ttttgttcca cgcgctgcgg tctgacgcgt
     1681 tccgtcctct ctgtcaaagc aatgtatgcc gccgacacca aaataatcga aacggcaagc
     1741 gcgcgcagcg cgtatttccg atgttttgcc gaaagtggga agacagccat attgttcctt
     1801 tttgacggcg tttgcgccga atcctctata atatccgccc gatccgcatt tgtcagacat
     1861 caaacgatga aactcaccct cgccctcccc tcgctcaatt tggatgaaga cgaaatccga
     1921 atacctctat gcctgcccgc cttcaacaaa atcctgcaat acggctcgct gcaccgccaa
     1981 agctgcacgg cttcggcatt ttacgcgcgt tatttgtggt gcggcagact ggcggaacgc
     2041 gcggcgcaaa gcctgaatat gccgtctgaa accgtcgcgc tcgccacgcc cgtctggcaa
     2101 aagatgggga tgcatcaggc aaatgtgctg acggcggaat atttggatgt cggtacggat
     2161 gaagcggaac ggctctgccg cgatctgtcg gcgttttacg gcgatattcc gtggcgtttc
     2221 gttcccgtcc tgcccgaatt atggctggtt tcccttcccc gcgcgtaccg ctggggcgcg
     2281 aagcccgttt tggacttggg cggattgttg ggcgcggacg atcagccgga cggagaagat
     2341 gcgttggaat ggctgcgcgt ccaaaccgaa atccaaatgt ggctggcggc gcaccccgtc
     2401 aaccataacc gcaagaaacg cgggctgccc gagttgaacg gcttatggct ttggggcggc
     2461 acaaacggcg gcacgcaagg cggcacgctt ttcgccgata cggtttggag ccgtttccac
     2521 cccgaccgcc gcgcattgcc cgacaacttc cgcgcctatg aagaaacggc ggcacacctg
     2581 cccgacacgc accatatcct gtttatggac gatttgcgcc tgaccgccct gacaggggac
     2641 agggagcggt atgcggcaat attgcagcag tgggaagaac gctggttcgc accgctttac
     2701 gaagccgtcc gcatcggcaa aatcaaacgg ctggacatcg ccacggacgg acaacacggc
     2761 ggtacgctga ccttcaaacc cgccgacagg tggaaatttt ggcgcggcgc gaaaaccttt
     2821 gacggaatct ggtgaacccg cccgccgcaa ccgcgacaat gccgtctgaa gcccgaatcg
     2881 ggtttcagac ggcattttac ctcgcatcgc cgttatgccg gaaaaaccgg aatgcgtttc
     2941 cgcacacgcg gcggcacgca aagcaccgcc cgaaagccgc cgatgcccgc cgtgataccg
     3001 ccgccggcag ggtttcctcc gaaaaggaac agcaatcaga acgttttatt aaactcgaca
     3061 aacgcccgat ttttctcgta ttcgttgaac acatcgttac tccgcgtttc gcggtgcgac
     3121 aacgtcaggc gcggcgtgat gcctttgaaa tgcaatgccc ggtgccaaag gctcaaggat
     3181 gtgttcaatt ctttatccct gcgcctttcc cctttaaaac cgctgaaaaa gccgggtttt
     3241 tcataatgcc gtttcgccgc gccgaggcgc aacagcgaag acaggccgct gccgccccat
     3301 tcctgccccc aggcaaagcg caggccgtaa cggttgaaat tgtcgccccg gtcggcgggg
     3361 ttgcgctcgc ggtaaaaatc caaaccgccc atccaatatt ggcgcgcatt ccggtaaaac
     3421 accagcgaat tggaaatttg caaatgggta ttgtcggaac gcgcccggcg cgtattcttc
     3481 aaacgccccc actccgccga agacaacgtt tgccatttcg gggtttgcca acggttgaaa
     3541 taaaggcgtg cgccgttggt gtaagaataa gcgtcgttgc cgtaggtgcg gcgttcgtgg
     3601 aacactgcca gcccggcatc tttgcgccgg tcggcaaaac cgatgccgcc ggaaacgcct
     3661 gccgtcatat cgttgaattt cttattcccc ggataaaccc tgccggacac gtcgccgccc
     3721 gccgtcgtgt accagccgtt tttcagcgac cattttttct ccgcgccgag ccggtaattg
     3781 accgccgtgc cgtccacctg tttcgggaaa gtccatttgc cgtactgctg ccgtttcggg
     3841 gcttggttga tattgtgttc gcgggtgacg ctgaagccgc catttacctt ccacgcatcg
     3901 cgttcgcgca atgccttgcg gtacagctcg acctgctcca tcagctgcgg cggcaggttt
     3961 tccgccttca ggcggtcgaa ctggtctgcc gccgcctcgt tctgcctgtt ttcaaacaat
     4021 gccgccgcca aacgcatacg gacggcgggc gcgtcgggtt gggcggcaat caattcccgg
     4081 taatgggaaa tcgcctcctt caccctaccg tctgcctgcg ccaaaatccc ttgtgcataa
     4141 agtgccaaca tcttatcctg ctgcgcctgt tgtaggtaaa tcggcaaaat aacgcggata
     4201 ccggcaatat tgtttgagac cactgcggaa tacatcgcgc gggacaacaa ttcgggattt
     4261 ttcagcaggg tttctccgtc aatctgcaaa acttttcctt tttcccgcac ctgccccggt
     4321 accttctccc tgtcgattgg tttgacctcc gcttcatgaa gcctgaactc gggacggctt
     4381 ctcaaatccg gttcgcgcgg tgtttcttcg gcatatgccg cgcttgccaa taaaggcagc
     4441 agcagcatcc atctgtttcg tgcaggcata aatgtcttcc cacaaaaata aaaaataacc
     4501 attatcaatc ggcggattgt atcagttttt tatgccgctg ttccaaaccc tgccgcccga
     4561 tgccgcgaca atgccgtctg aagccagaat cgggtttcaa acggcggcaa aaaaccggcg
     4621 gttttccgcc ggtttcatca tgatgccccg cccttcgggg cggcagggtt cggattggtt
     4681 tgcggggcgg cgcgcggggc cgtgccggtg tgccgcctgt gcgtcaaaac atttcccgac
     4741 gcatcaaaac agccatttga gcgacaatgc ggcttctttg tcgccgtccg tccttttgcc
     4801 gtatccgatg cgtgcggaca tatggccttt ccaaccggct tcaatgccga accgcccttc
     4861 gagcgccgtc ctgcctgcca gcgtctgttt ttcgccgtcc atttccacgc cgaaagattt
     4921 tgacctgtgc aaaacattaa aagcggcgaa aggctgaaga ttgacaccgt tacgcaaagc
     4981 aaaacgggtt tttgcccgaa taccggcgcg gctttgccac tgaccgctgc cgagcagtcc
     5041 gaccgccgtc ccctcgctgt cggtaaagcc gccgtttacg cccaagtagg taaactgagc
     5101 ctgcggttgc aggtaaaacc gcacattatt gccttttccg acaacgcctt ccgccacaag
     5161 cgcgttgtag ccgccttcga cagaagccgt ccaacctttg gttttgtagc gttccacacg
     5221 gttttcatca ttgatgcggt gtttgaaacg ttggtattgc aaccagccgt ccaaatacgc
     5281 acccgtttgt ttatcgcgca actgatgcca cgcagcataa acacccccgc cataaccatg
     5341 caaataactg cctgccgcac cgcctttgcc gttgactgat gcgtgctggc cggccctgcc
     5401 gcccatcacg ccgattgcca gccggctgcc ttcattttgc cgtacaaaca cctcgccgcc
     5461 gatttgcacg cctttgcgcc gcccgtccgc agccgcgccg ccccgtatat tttgatgcga
     5521 ccggccgccg atgaagcgca gccacaattt ttgacgggac ggatcggcgg catacacgtc
     5581 gtctcccctg tcggcggcac gcagcccaaa caaagtattt gccgcataag cctgttgggc
     5641 atataaaacc gcttccgcct cggggatagc caaacgctgc acgcccaagc aaaatccgcc
     5701 ttcgcattgc ttgagcaata attcgtacag tccctgccgg taattttgtt taaacgcaaa
     5761 cgaattaggg tctgcctttt ccgccgcaat cagtttgcgc ccgtcgagcg ttttcagatt
     5821 ttccggccgg cgttccaaaa ccacgccgga ttgtccgctg actttgtctt caaaggtcag
     5881 ggcgggttcg tccgactccc gcacgtcgta gccgaacagg aaatccgcgc cgccctgata
     5941 cgctttatgg acggtcagtt tttcatcttt cgtgttcagg cggatccggg cattatcgga
     6001 agtttccagc atatggatgt cggaatggcg gcgcggttcc caaagggaat ctttcagcgt
     6061 catcttggtc agttccaaca cctgtccggc gacgtgggat ttgttttcga ttttgacgtg
     6121 taatttttta ttgaagatgt tgttgagact agtctgtgat gtattactat gaggagtgag
     6181 tacggaatct tctaccaatt tattaattaa atcggggttt ttctgtttga gtatactaaa
     6241 ttttggtcca agctgttcta tatacgcctc ctcagtccgt tttttatttt cttcccaagc
     6301 ttcgggtctt gttttgtata aatcctgtaa ttgcttctta accgcatcct ttaaattatc
     6361 cccgtaagta tctgtaatat atttcttaac aacgggcata ttatcgacgg cgagtatgcc
     6421 gctattgtag tttttacctt ggatattgat ttcgtcatac ccatattcgg atgaattttg
     6481 atcggtttta ccgctggaaa aataatggga agtgccgggt ttcggcttgt ctgaaaatga
     6541 aaaatcggca tcgtcgccga ttttggtaga acgagtattt gaaccaagct gagctctgaa
     6601 cataccgaag caatcaaaag atatgctgca cgggccgcgc caaacgtggg aatttctgtc
     6661 ttccgccgct tctaaaataa atttgttttt aatctcggct tctttgacgg gatcgtacgc
     6721 atccgaacct caaacatatg gcgtgcaaaa cagcgataaa acagataatg ttaatagatt
     6781 aacccgaccg cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct
     6841 tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct ttcagtgtca tgagaaattt
     6901 tcctttgtca agtgttaaaa ttataatgat tatatactac attatactac atcgcaatga
     6961 aaaatcagga aaagcaaaaa accctccgcc gtcattcccg cgaaagcggg aatctagaat
     7021 tttaatgccg caagaattta tcaaggatgg tcggggttta aaggtctgga ttcccgcctg
     7081 cgcgggaatg acggtttaga aatcacccga aacaagaaaa agaaccgaaa ccgaacaagc
     7141 cggattcccg cctgcgcggg aatgacggga tttcaggtta ctgtttttga ttttctgttt
     7201 ttgtgggaat aacgggactt tggatgatgt gcatttatcg agaaaactaa aaaccctccg
     7261 ccgtcattcc cgcgaaagcg ggaatctaga cctttcggtg cggaaactta tcggataaaa
     7321 cggttttccc tgcatcctgg attcccactt tcgtgcgaat gacgggatat aggtttcact
     7381 acggacgtgt tcagattacc gcctgcgcgg gaaagacggg atttcaggtt gctgtttttg
     7441 attttctgtt tttgtgggaa taacgggact ttggatgatg tgcatttatc gggaaaacaa
     7501 aaaaccctcc gccgtcattc ccgcgaaagc gggaatctag aattttaatg ctgcaagaat
     7561 ttatcgggga tgaccgaggt ttgaaggtct ggattcccgc ctgcgcggga atgacggttt
     7621 agaaatcacc tgaaacgaga aaaagaaccg aaaccggaca agtcggattc ccgcacgggc
     7681 gggaaggacg gaatttcaga ttgttgtttt tgattttctg ttttgtggga ataacgggat
     7741 tttggatgat gtgcatttat cgggaaaact aaaaaccctc cgccgtcatt cccgtgaaag
     7801 cgggaatcta gaatttcaat gccgcaagaa tttatcgggg atgtccgggg tttaaaggtc
     7861 tggattcccg cctgcacggg aatgacggtt tagaaatcac ctgaaacgag aaaaagaacc
     7921 gaaaccagat aagtcggatt cccgcacggg agggaaggac gggatttcag gttactgttt
     7981 ttgattttct gttttgtggg aataacggga ttttggatga tgtgcatttt cgggaaaact
     8041 aaaaaccctc cgccgtcatt cccgcgaaag cgggaatcta gaattttaat gcggcaaaaa
     8101 tttatcggaa aaaaccgaag tttaaagacc tagattcccg cctgcgcggg aatgacggtt
     8161 tagaaatcac ccgaaacgag aaaaagaacc gaaaccgaac aagccggatt accgcctgcg
     8221 cgggaatgac ggctcaaaag ttacgaaacg aaaaataacc gaaaccagac aagtcggatt
     8281 cccgcgcggg cgggaataac gataagcgta atttcaatct gccatattgt cttttcactt
     8341 gcctgaaaac agatttcagc cgcctatccc tgcatccggc ggctgatgta gtcggcaatc
     8401 aaatcccgct tttcccaaaa aaatttctgc attttttgtt cggaatacag tttgcggacc
     8461 agagtgggca gaccgctgtg gacgatggcg gcgatttctt tgttgccttc ttcttcctgc
     8521 attgccgtct gaaaatccat gcccctgcct ttcagatatg ctgcaaggcg gttgcggaca
     8581 tgcgaattga gttcgataga tttgagcatt tttcctccgt tttcgataac gattatattt
     8641 aatccgtctg tatgccttat tttatcatgt ttcacgatcg gtttgcctgt acaataccgc
     8701 ttttcagtat gatgtgctgg catttatgta ttcttggttc aagctgtttc acttgttttt
     8761 tgtcatttcg tggtttgcag ggctgtttta cctgccgagg attttcgtca atatggcgat
     8821 gattgatgcg ccgcgcggca atcccgagta tgtgcgcctg tcggatatgg cggtgcggct
     8881 gtaccgtttt atgtcgccgt tgggcttcgg cgcggtcgtg ttcggcgcgg cgataccgtt
     8941 tgccgccggc tggtggggca gcggctgggt acacgtcaaa ctgtgtttgg gcttgatact
     9001 cttggcttac cagttgtatt gcggcgtgct gctgcgccgt tttcaggatt acagcaatgc
     9061 tttttcacac cgctggtacc gcgtgttcaa cgaaatcccc gtgctgctga tggttgccgc
     9121 gctgtatctg gtcgtgttca aaccgttttg acggagcatc ttatgccgat agaaatcgaa
     9181 cgccgttttt tgattggaaa cgacaactgg cggcaatacg ccgacgaacc gctgctgttg
     9241 aggcagggtt atctgtctgt tgaaaaagaa cgcaccgtcc gtgtccgcat tgccggacaa
     9301 cgggcgtggc tgacgctgaa aggctatatt tcggaaatca gccgcagtga gtttgaatac
     9361 gaaatcccgc ttgccgatgc ggaaaagatg atggaaacga tgtgcccgtt taaaatggaa
     9421 aaatggcgtt atccggtcag atggggcggc agcctgtttg aaatcgatgt tttccttggc
     9481 gaaaacgcgc ctttggtcgt cgcggaaatc gagttgcccg acgagaatgc cgattttgac
     9541 cgtccggact ggctggggcg cgaaatcact gccgacggta tgtttaccaa tgcttatttg
     9601 agcaggcatc cgttctcaag ctggaaaaat gccgtctgaa acggcttcag acggcattct
     9661 ccgacaacca tcgcaaatcc aaagacagcc tctatatcct tatttctgct ttctttgttc
     9721 cattattccc aataaatccc cataacctaa aatctcgtca ttcccgcgca ggcgggaatc
     9781 cggttcttta aacttcggtt ttttccgata aattgcttta gcttttcgtt tccggattcc
     9841 cgcttttgcg ggaatgacga tggaagattg ttgttatttc ggataaatta ccctaacttt
     9901 aaaatcccgg attcccatct tcgggggaat aacaatattt tctatttcag cccatcattt
     9961 gcgggggatt ttatagagag tttatatttc aatatctcaa ataatgcaca aaagcatagc
    10021 gcgtgccttt gctgctgacg cggcgttccg tccgccctgc ctctttccaa tgcgtccggt
    10081 ctatttcggg gaaaaacgca tctccttcca cagacaaatc cacttcggtt atccgcaaat
    10141 cggtcgccaa tggcatcgct tgtccgtata tctgcgcgcc gcccataatg acggcttctt
    10201 ccgcgcctgc gcacaatgcc aatgccgcct ccaaacttgc cgccgtttcc gcgcctgccg
    10261 cgcaataatc cgcctgccgg ctgatgacga tgttcctccg tccgggcagg ggtttgacgg
    10321 gcagggattc ccacgttttc cgccccataa tgacgggttt gcccaaggta taggctttga
    10381 aaaatgcgaa atcttcgggg atgtgccaag gcatagcatt gcccgccccg atgcacaggt
    10441 tttccgcaca cgccgcaatt agggttattt tgagcatatt tcctccacaa aaatgccgtc
    10501 tgaacgcttc agacggcatt ttatgacatt ccgtgtcaaa aatctcaacc ggcgcgcgcc
    10561 atacgttttt tgtttgcacc tgccaacaat gccaacagtt cttcagtcgc gccccaaccg
    10621 atacacgcat cggtaatgct tttgccgtac acttccggct tatcctgtct gccttcgacc
    10681 aaatggcttt ccaccatcac gcccataata ttgctcccgt cctgttccaa ttgggcagca
    10741 atatcttgtg ccacttccat ctgccgggtg taatccttgc ggctgttggc gtggctgcaa
    10801 tctatcatca gcttatcggt tacccctgcc gcacgcagtt gttccgccgc ctcgctgacg
    10861 tgtcccgcat cataattcgg ctcttttccg ccgcgcaaaa tgacatgaca gtcgggattg
    10921 ccgccggtat ggacaatggc ggaatgtccg gccttggtta cagacaagaa atgatgcgaa
    10981 tggctcgccg caccgattgc gtcgatggca atcttcaaat tgccgtccgt accgttttta
    11041 aagccgacgg ggcaggacag cccgcttgcc aattcgcggt gaacttggct ttcggtcgtc
    11101 cgcgcaccga ttgcccccca agaaatcaag tccgcataat attgcggcgt aatcatatcc
    11161 aaaaactcgg tagaggcagg catacccata ttgttcagcg acaacaacag gctgcgcgcc
    11221 tgacgcaaac cgaaattgat atcgaacgta ccgtccaaat gcgggtcgtt aatcaaacct
    11281 ttccaaccca ccgtcgtcct cggcttctcg aaataaacgc gcatcacaat caaaagctcg
    11341 ttttcatact gcttgcggag tttcaacaaa cgctccgcat attccaacgc cgctttcgga
    11401 tcgtgaatcg aacacggccc gataataacc aacagccgct tgtccctgcc gtgaaccaaa
    11461 tcggaaattt cctgacgggt gcggtgaacc aagcccgaag cctctttgga aatcggcagc
    11521 tcgtaaagat gggcaatcgg cggcaacaac tctttaactt ctttaatctt aatatcgtct
    11581 gtggggtaat ggtgtgtcat cttgcatctt tcctgatggt tttttatacg cggcttaggt
    11641 tacatcgttt accttgcaca aacaagtgca acatattact acaaagctat tttatttcaa
    11701 aataactgat atatcttttt tcatagattt taaatttcat atttttaata ttttaggaat
    11761 tttattccaa agaaaaagaa acagattgat aaaaacaata cccgccattc attcggacaa
    11821 aaccgcacgg aacatccgtt ttcccatttc cacgacaatc cggtacagaa caccccgctg
    11881 ccgattccgc ctgcacatcc ggcaaaattt catacacaag gaaaatgttg aaaacaaaaa
    11941 gaactcattg cttgaaaaaa gatttaatgg gaatgcgggt atttcccaca ctttttccat
    12001 ttatttatcc tcattatagg aagaaaaaca tgaaaaaacc ctgcttgcac tctcacttgc
    12061 agccgtgtcc gccgttacgg tagccgatat tcccgagtct aacggcaaag gcaaagccat
    12121 catcggcgat aaggaaaaac accgttacca agcatgaaaa ccttgctttg aaagtccatg
    12181 gcgaaatccg ccaagatttg ggggacggat gatccctaac cccttgggcg gatgcatatg
    12241 cagatttgcg cggcaaaacc aaagtgatga cgacccagat gtgtgcttcc cgcgatgtca
    12301 gcaaaagcgc caaaggttgg agtgtcggta tcggtctgaa tgtaggcaaa cagttgaccg
    12361 acagcgtcgg tctcgagttt gatccatact accgtcacaa aacaatctgc aaaccccgtg
    12421 agattgtttt ggacggcgac aaaaccaaaa tgggccgctc caaatccaac gagtacggct
    12481 tccgcgtagc cgcaacgttc tatagtcaat taaaatcaaa ataggacagt agtgcatcgt
    12541 caaatcgggc gtaatcagac aaaacggttc gcagataccg cttaatattc gcccacactt
    12601 tctcaatcgg gttgagctca ggtgaataag gtgcaagagg
//
LOCUS       DAXHTR010000055        12313 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.055, whole genome shotgun sequence.
ACCESSION   DAXHTR010000055 DAXHTR010000000
VERSION     DAXHTR010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12313
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.055"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(260..1189)
                     /locus_tag="ACFNML_001034"
     CDS             complement(260..1189)
                     /locus_tag="ACFNML_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6392437.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNDIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELEPV"
     gene            1320..2069
                     /locus_tag="ACFNML_001035"
     CDS             1320..2069
                     /locus_tag="ACFNML_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HFC6392438.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRTAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            2192..2773
                     /gene="petA"
                     /locus_tag="ACFNML_001036"
     CDS             2192..2773
                     /gene="petA"
                     /locus_tag="ACFNML_001036"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HFC6392439.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            2792..4141
                     /locus_tag="ACFNML_001037"
     CDS             2792..4141
                     /locus_tag="ACFNML_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HFC6392440.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCAVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            4156..4944
                     /locus_tag="ACFNML_001038"
     CDS             4156..4944
                     /locus_tag="ACFNML_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HFC6392441.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            5283..7067
                     /gene="rmuC"
                     /locus_tag="ACFNML_001039"
     CDS             5283..7067
                     /gene="rmuC"
                     /locus_tag="ACFNML_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFC6392442.1"
                     /translation="MELMTVLLPLAALLSGVLFTWLLMKSRFQGVLAVLNAQLAEKAA
                     RCDFVEQAHAEIASELAVLDGKYEHLQDENYALGNRFSAAEKQIAYLQEKEAESVRLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLSDVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAAAVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQNDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKANKQ
                     LARDLTEHANETTALSESLEYAAEDEAV"
     gene            complement(7274..7906)
                     /locus_tag="ACFNML_001040"
     CDS             complement(7274..7906)
                     /locus_tag="ACFNML_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HFC6392443.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTRKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFSPNRWLEEGETLTVGRYAFQVLHIPGHTPGHVVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFALPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            7998..8822
                     /locus_tag="ACFNML_001041"
     CDS             7998..8822
                     /locus_tag="ACFNML_001041"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HFC6392444.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFTPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQTKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     IGCKNRHGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTRKGTPKFATFVRVRTDR"
     gene            8891..9454
                     /locus_tag="ACFNML_001042"
     CDS             8891..9454
                     /locus_tag="ACFNML_001042"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HFC6392445.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            9535..9972
                     /locus_tag="ACFNML_001043"
     CDS             9535..9972
                     /locus_tag="ACFNML_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFC6392446.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            10041..10310
                     /locus_tag="ACFNML_001044"
     CDS             10041..10310
                     /locus_tag="ACFNML_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFC6392447.1"
                     /translation="MLKQSIEIINKLGLHARASNKFTQTASQFKSEVWVTKNDSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            10310..12085
                     /gene="ptsP"
                     /locus_tag="ACFNML_001045"
     CDS             10310..12085
                     /gene="ptsP"
                     /locus_tag="ACFNML_001045"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFC6392448.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTAQRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPAVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRVLLGMGLRRFSMNPNNILPVKNIILHSNVGQLENEIAKIVRCEDE
                     EKAEKLIKQINSVSVEEEADFKGRK"
BASE COUNT         3026 a         3075 c         3454 g         2758 t
ORIGIN      
        1 tatttggggc gaaaacagct gaaaacagct gaaaacctgt gtttgggttt cggctgtcgg
       61 gagggaaagg aattttgcaa aggtctcaga ttgggcaaaa atcaaaaaca gtatagtgga
      121 ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggcaaggcg
      181 aggcaacgct gtactggttt ttgttaatcc actatacatg aaaaaataga aaaactgccg
      241 ggtatggttg aataaggggt cagaccggtt ccaattcgct cagtccgggc aaatctgcaa
      301 aaccgcgttc gcgtatgatt tggcaaaaat tgttcagata gctcttgtcc gtatcttcgg
      361 tacggatggc ggcatacagt ttgctttgca atccgtcggc agtaatttgg cggtggacga
      421 catagccttt ttcaaggtag ggcatgactg tccaataggg aagggcggca atgccacgtc
      481 tgctggcaac cagttggata atggcgatgg tcagttcgct gtgtcggcgc ggcgggttga
      541 tgtttttcgg aatcaggatt tttttgggca aatccagcat ctcgtcggga acgggataag
      601 taatcagggt ttccccgata aagtcttccg ccgtccaaac gtttttggcg gcaagcggat
      661 ggtctggtgc gcaaatgccg accatttcgt aggcaaacag cggttggaaa gaaatatcgt
      721 tttgtttttc cgcttcggaa acaatggcaa ggtcggcacg gtgttgcagc agcagtccga
      781 cgggatccgc ttggaatccc gatacgatat ccaattcgac ttggggccac atcgggcgga
      841 attcgcccat ggcgggcatc agccagtcga aacaggtatg gcattcgacg gcaatccgca
      901 gctctcccgc ctctccttcc gtgattcgcg ccaaatccca ttctgcaaca gcaacttgag
      961 gtataagttc gtgggcgagg cgcagcagcc tttcgcccac cggggtaaag cgcaagggcg
     1021 tggatttgcg ttcgaacagc ggcgtgccgt agtggttttc gagcatacgg atctggtggg
     1081 aaagagcgga ttgggtaagg aaaacccgtt tggcggcaag ggagacgctg ccggtttctt
     1141 caagtgccag cagggtcttg aggtggcgca gttcgataat ggaatccatg gggcttcaga
     1201 cggcatattg aaccggcgca tattaaaata attcatatgc gggtgcaaat cggaaaaatg
     1261 tgaattccgg acattttgcg attagaatgc ccgcttgttt aaagcgatta ggaaaaaaga
     1321 tggtattgcg cagggatttt ctgacttggt gtaatgaaac attgcagaca gcgttgttta
     1381 aagattacgc ccctaacggt ttgcaggttg aagggaggga atatataggg aaaatcgtta
     1441 cgtcggtaac ggcaagcagg acagcgattg attttgctgt ggagcagaag gcagatttgc
     1501 ttttggtaca tcacggtatg ttttggaaaa acgagttgcc gaccgttact ggttggaaaa
     1561 aagaacggat tgccgcactg ttacggcacg acatcaatat ggcaggctac catctgcccc
     1621 tggatgcaca tcccacactg ggcaacaatg cccaactcgc cgacagattg ggttttgcga
     1681 cagaaaaacg gttcggcgaa caaaacctgc tcaactcggg cagcctgaaa caagccaaga
     1741 cactcggcgc attggcggcg catattgaaa cagttttgca acgtaagcct gtcgtcatcg
     1801 gcaaacccga acgcgaaatc cgacgggttg catggtgcag cggcggggcg caggggtttt
     1861 ttcagacggc aatagacgaa ggtgtcgatc tgtatctgac gggggaaatc tctgaagccc
     1921 aataccatct cgccaatgaa acgggtacgg ctttcatttc ggcagggcat cacgcgacgg
     1981 aacgttacgg cgtacgcgca ctggcagaat cggcggcaga ggttttcggg ttggaagtgt
     2041 gccattttga cgaaaacaat ccggcttgag tcttgagaaa tatcataaaa ctttacctta
     2101 ttttaaataa tgctttgagt atcactgcaa actgggtaaa attgcaacgt ttaatggctc
     2161 ggtattccca aaatattagg acgactgaat aatggataat caagaaatca acaacggccg
     2221 ccgccgtttc ctgacactcg cgacctgcgg cgcgggcgga gtggcagcat tgggtgtggc
     2281 aacgccgttt gtggccagtt ttttcccttc ggaaaaagcc aaggccgccg gtgctgccgt
     2341 cgaggtggat gtcagtaaaa tcgaagcggg tcagctgctg accgccgagt ggcaaggcaa
     2401 accgatttgg gtgctcaacc gtacagatca gcagcttaaa gacctgaaag gcctgaacgg
     2461 cgaacttacc gatcccaatt ccgatgcgga acagcagccg gagtatgcta aaaacgagac
     2521 ccgttcgatt aagccgaaca tccttgtcgc catcggtatc tgcacccatt tgggctgctc
     2581 gcccaccttc cgtcccgaca ttgcccctgc cgatttgggt gcagactgga aaggcggctt
     2641 cttctgcccc tgccacggtt cgaaattcga cttggccggc cgcgtatata aaggtgttcc
     2701 tgccccgacc aacctggttg tcccgccata taaatacttg agcgacacaa ctatcttggt
     2761 gggcgaagac taagaataag gaacgataat tatggcaaac caaaccaata gcaaagcaaa
     2821 agcattgtta ggctggatgg acgctcgttt ccccctgtct aaaatgtgga atgagcattt
     2881 ggctcaatac tacgcaccga aaaactttaa tttttggtac tacttcggct ctttggccct
     2941 gcttgtcctc gtgattcaaa tcgtcagcgg cattttcctg accatgaact acaaacccga
     3001 cggcaacctg aacgcctacc atctgcctgc tgcctttact gcagtcgagt acatcatgcg
     3061 cgacgtgtcc ggcggttgga ttatccgcta catgcattcc accggcgcat ctttcttctt
     3121 cattgttgtt tatctgcaca tgttccgtgg tttgatttac ggttcgtaca aaaaaccccg
     3181 tgagttggtg tggattttcg gttctctgat tttcttggca ttgatggcgg aagcctttat
     3241 gggctacctg ctgccttggg gtcagatgtc cttttggggt gcgcaggtaa ttattaacct
     3301 gttctccgcc atccctgtta tcggtccgga tttgtccact tggattcgcg gcgacttcaa
     3361 cgtttccgac gttaccctga accgcttctt tgccctgcac gttatcgcgg tacctttggt
     3421 attgctcggc ttggttgtgg cgcacatcat cgccctgcac gaagtgggtt ctaacaaccc
     3481 tgacggcgtt gaaatcaaga aaaacaaaga cgaaaacggt atcccgcgcg acggtatccc
     3541 attccatcct tactataccg ttaaagacat cttgggtgtg gttgtattcc tgattgtctt
     3601 ctgtgccgta ttgttcttcg caccggaagg cggcggctac ttcctcgaag cgccgaactt
     3661 cgatgcggca aatgcgctga aaacacctcc gcacattgcg ccggtatggt atttcacacc
     3721 gttctacgcc attttgcgtg cgattccctc gttcttggga acgcaggttt ggggtgtaat
     3781 cggtatgggc gcggcggtcg tgttgattgc cctgctgcct tggctggata ggggcgaggt
     3841 gaaatccgtc cgttaccgcg gcccgatttt caaaaccgca ttggtcttgt tcatcatttc
     3901 ctttatcggt ttgggtattt tgggcgcgat ggtggcaacc gatacgcgta ctttggtcgc
     3961 gcgcatcctg tccttcgtct atttcgcatt cttcctgggt atgccgttct ataccaagct
     4021 ggataaaaac aaaccggttc ccgaacgcgt aaccatgagc accgccaaac aaaaaatcat
     4081 gttctttgtt tacgtcggca ttaccgccgt gggtgcttac ctgtttgcaa ccaacatctg
     4141 atgagggcag tcaaaatgaa acaagctatg aaaaattggt ttgctgcctt gctgttggca
     4201 gtacccatgg gtgcggcatt tgcttcgggc ggtcatgcac actatgagaa agtcgatatc
     4261 gatttgcgcg accaagtcag cctgcagcgc ggcgcacaaa tctttaccaa ctattgcctg
     4321 tcctgccact cggcaagcgg tatgcgtttc aaccgtttga aagacatcgg tttgactgac
     4381 gaagaaatca agaaaaacct gatgtttacc accgataatg tcggcgatgt catgcattcg
     4441 gcgatgaacc cgaaagatgc ggcaaaatgg tttggtgctg ctccgcccga tttgacattg
     4501 attgcgcgtt ccaaaggtgc agactacctt tacgcttata tgcgcggctt ctataaagat
     4561 ccgacccgtc cgagcggctg gaacaatact gtattcgata aagtcggtat gccccacccg
     4621 ttgtgggaac agcaaggcgt tcaagccgtt gagttggatg ccaaaggtca gccggttatg
     4681 gtaaaagacg aacacggcga gatgaagcct aagctgtatt gggaatctac cggtttgcac
     4741 agccgccgtc tgcctaacgg caaagtgatc caaaaagagt acgacgcata tgtacgcgat
     4801 ttggtcaatt accttgtgta catgggcgaa cctgcacaac tgcaacgcaa acgtataggc
     4861 tatgtcgtga tgattttcct atttgcggtt atgctgcctt tggcgtattt cctgaaaaaa
     4921 gaatattgga aagacgtaca ctaagcgttt ggaacaaaag ggcaaatcct ttagggtttg
     4981 cccttttttc attttgcctg ccgtttgaaa agcctgaatc cgtatgccgt ctgaacatag
     5041 ctgcaacatt tcagacggca tcattctaaa aatgtcagac atcgggaact taatcagggt
     5101 ttgtggcaga ttgatattga attaggaaaa ggtctgaaac cgcaagcagc atggtaaggc
     5161 gatgtcgaac aggtttaaag ccaatccgct atattttttg gagctatgtc gaacaaacag
     5221 gtagaatcgg aataagcggc ggttttgtcc ggtgtgaaaa tgtttgaata ttaggataat
     5281 tgatggaact gatgactgtt ttgctgcctt tggcggcgtt gctgtcaggc gtgttgttta
     5341 catggttact gatgaagagc cggtttcaag gtgtattagc cgtattgaac gcgcagttgg
     5401 cggaaaaggc ggcaagatgt gattttgttg aacaggcaca cgcagaaatt gcatcggaat
     5461 tggccgtttt ggatgggaaa tacgagcatt tgcaggacga aaattatgct ttgggcaacc
     5521 gtttttccgc agccgaaaag cagattgctt atttgcagga aaaagaggcg gaatcggttc
     5581 ggctgaagca gtcgtatatc gagttgcagg aaaaggcaca gggtttggcg gttgaaaacg
     5641 aacgtttggc aacgcagctc ggacaggaac ggaaggcgtt tgccgaccaa tatgccttgg
     5701 aacgccaaat ccgccaaaga atcgaaaccg atttggaaga aagccgccaa actgtccgcg
     5761 acgtgcaaaa cgacctttcc gatgtcggca accgttttgc cgcagccgaa aaacagattg
     5821 cccatttgca ggaaaaagag gcggaagcgg agcggttgag gcagtcgcat accgagttgc
     5881 aggaaaaggc acagggtttg gcggttgaaa acgaacgttt ggcaacgcaa atcgaacagg
     5941 aacgccttgc ttctgaagag aagctgtcct tgctgggcga ggcgcgcaaa agtttgagcg
     6001 atcagtttca aaatcttgcc aacacgattt tggaagaaaa aagccgccgt tttaccgagc
     6061 agaaccgcga gcagctccat caggttttga acccgttgaa cgaacgcatc cacggtttcg
     6121 gcgagttggt caagcaaacc tatgataaag aatcgcgcga gcgactgacg ttggaaaacg
     6181 aattgaaacg gcttcagggg ctgaacgcgc agctgcacag cgaggcaaag gcactgacca
     6241 acgcgctgac cggtacgcag aacaaggttc agggtaattg gggcgagatg attctggaaa
     6301 cggttttgga aaattccggc cttcagaaag gacgggaata tgtggtgcag gcggcggctg
     6361 tccgcaaaga agaagacggc ggcacgcgcc gcctgcagcc cgacgttttg gtcaacctgc
     6421 ccgacaacaa gcagattgtg attgattcca aggtttcgct gacggcttat gtgcgctata
     6481 cgcaggcggc ggatgcggat acggcggcgc gcgaactggc ggcacacgtt gccagcatac
     6541 gcgcgcatat gaaaggcttg tcgctgaagg attacaccga tttggaaggt gtgaacactt
     6601 tggatttcgt ctttatgttt atccccgtcg aaccggccta cctgttggcg ttgcagaatg
     6661 acgcgggctt gttccaagag tgtttcgaca aacggattat gctggtcggc cccagtacgc
     6721 tgctggcgac tttgcggacg gtggcgaata tttggcgcaa cgaacagcaa aatcagaacg
     6781 cactggcgat tgcggacgaa ggcggcaagc tgtatgacaa gtttgtcggc ttcgtacaga
     6841 cgctcgaaag cgtcggcaaa ggcatcgatc aggcgcaaag cagctttcag acggcattca
     6901 agcagcttgc cgagggacgc gggaatctgg ttggccgcgc cgagaaactg cgtctgttgg
     6961 gtgtgaaggc aaacaagcag cttgcacgcg atttgaccga acatgccaat gaaacaacgg
     7021 cgttgtcgga atctttggaa tatgcggcag aagatgaagc agtctgactt gtgcggaaaa
     7081 atattgtttc agccggagcg ggaatgccga aagcgtggcg taactgtacc ggttctgatt
     7141 tggggtttgt ttgaagtact taccaaagcc ttgtctggcg aggtacaccg gcaaggcagc
     7201 ggttgtctga gccttttgtg tttcagacgg catcagtgca gatgatgccg tctgaagacc
     7261 gtagtgaaag cggttagaaa aacggattgt gccgtttttc gtgtccgatg gaagtcatac
     7321 gcccgtgtcc ggcgacaact tgcacggttt cggggagggc gaataatttg ttgcggatat
     7381 tattgattaa gtcggcgtgg ttgccgcgcg gaaaatcggt tctgcctatg gtttcgtaaa
     7441 acagcacgtc gcccgcaatc agcaattccg cctcggcgca ataaaagacg acatgtcccg
     7501 gcgtatggcc cggaatatgc agcacttgaa aggcatagcg tccgaccgtg agcgtttcgc
     7561 cttcttcgag ccaacggttc ggcgaaaatg cgggcgagac gggaaatccg tattgcgcgg
     7621 tagtttgcgg cagcgattgg agcaggaatt catcgtccgg atgcggcccg aggacgggaa
     7681 ctttacgcgt tttcagcatt tcgaccacgc cgcctgcatg atcgagatga ccgtgcgtca
     7741 gccagattgc cgtgagcgta agtttgcggt ttgccaacgc ttgcagcagg aacggcacgt
     7801 cgccgccgac atcggtcagg acggcttcgc cgctttcgtc gtcccaaatc agggtgcagt
     7861 tttggcggaa gggggtaacg gggaagattt cgtaacgtag ggtcatcggc gtgttcctaa
     7921 acggtttttc agacggcatc gggtttgccg tttgttttat ggcggtttgc cgcccgtttt
     7981 gatattgggg gaatcagatg attaagaaga caatcggcgg catcataccg atttttacgg
     8041 cggttttcat ccctgcatcg gcaggcgcgg cggatttgat gctggcgcag gaatacaaag
     8101 ggcaggatat tgccggctgg gcaatgagcg agaaactcga cggcgtgcgc gcctattggg
     8161 acggaaagca cctgataagc cgtcagggct acgcgtttac gccccccaaa ggctttaccg
     8221 cgcagtttcc gccttatcct ttggacggcg aattgtatag cggacgtggt cagttcgagc
     8281 agatttccgc taccgtgcgt tctgtttctt cagactggcg cggcatccgc ctgcacgttt
     8341 tcgatgtacc caaggcgcag ggcaatctct accaacgttt ggcagtcgca acgcagtggc
     8401 tgaaaacgca tccgaacgcg ccgattacca tcatcccgca aaccaaagtg cgcgaccggc
     8461 agcacgcgat ggacttttta aaacaaatcg aagcgcaggg cggcgaaggc gtgatgctgc
     8521 gtcagcccga atcccgttac agcggcggca ggagcagcca attattgaag ctgaaaagcc
     8581 aatacgacga cgaatgcacg gtaacgcggc actatgaggg caaagggcga aacgccggac
     8641 ggttgggcgc gatcggctgc aaaaaccggc acggcgaatt ccgcatcggc agcggtttca
     8701 aagacaaaga ccgcgacaac ccgcccaaaa tcggcacact gattacctac cgctaccgtg
     8761 gttttacgcg gaaaggcacg ccgaaatttg ccacatttgt gcgcgtgcgt accgaccgct
     8821 gagcaggacg gttcagacgg catccgatac tttggtttat aattttcctt ttaccgaccg
     8881 attccgacat atgaccgatt tagaaaccaa acgccttgaa acacaggcga tgcttgaaaa
     8941 cgccgatctt ttgttcgacc aaggccaatg ccgtgccgca ctgcaaaaag tggcggacga
     9001 gattacgcgc gatttgggcg gcaaatatcc gctgctgctg cccgtgatgg gcggcgcggt
     9061 ggtgtttacg gggcagttgc tgccgctgtt gcgttttccc ttagattttg attatgttca
     9121 cgtttcccgt tacggcgaca agctggaggg cggcgcgttc aactggaagc gtatgcccga
     9181 tgcggaacaa atccggggca ggcacgtcgt cgtgctggac gatattttgg acgaagggca
     9241 tacgatgtcc gccattcaag ccaaactttt ggaaatgggt gcggcaagct gccgtgcggc
     9301 ggtgttcgcc aacaagctga ttgacaaacc caagcctatc cgagccgatt atgtcggact
     9361 ggatgtgccg aaccgttatg ttttcggtta cggcatggat gcggcgggct gctggcgcaa
     9421 tttgggcgag atttacgcat tggacggaaa ataaggacgc gatgccgtct gaaggctgtt
     9481 cagacggcat tgcgcccata cgccggcagg acaacgagga acaggacgca taatatgata
     9541 gggcttttaa tcatcacaca cgaaaccata ggcgaagcct accgcaagct ggcacatcat
     9601 ttttttccgg gcggactgcc tgaaaacgtc cgcatactcg gcgtgcagcc gacggaagac
     9661 caagacgaca tcatcaacaa cgccattgcc gcgcttcagg agtttcccga caacgacggc
     9721 gtgctaatta tgaccgatat tttcggcgcg accccctgca atgcagcccg ccgcctcgtg
     9781 cgcgaaaaca aatcggcgat tttgaccggg ctgaacgcgc cgatgatggt taaggccgtc
     9841 caatattcgc cggcggcgga agaccttgcc gcctttaccg aatgcgtcag ggaggcggct
     9901 gtaaaaggca ttttcgccat cacgtccgcg cccgaagatt tggtgtgccg gcgcagcggc
     9961 gatgccgtct gaagaagcgg cagggcagga aaacatttta aacggcgtct gctgccgata
    10021 tacataacac gggaaccgaa atgctcaaac aatccatcga aatcatcaac aaactcggac
    10081 tccacgcccg cgcgtccaac aagttcaccc aaaccgcgtc ccaattcaaa agcgaagtct
    10141 gggttacgaa aaacgacagc cgcgtcaacg gcaaaagcat tatggggctg atgatgctcg
    10201 ccgccgccaa gggtacggtc atcgaactgg agacggacgg cgcggacgag gcggaagcga
    10261 tgcgcgccct gaccgactta atcaacggct acttcggcga gggcgaataa tgagtatcgt
    10321 gctgcacggc gtggcggcgg gcaaaggcat tgccgtcggt tgcgcccacc tgattgcgcg
    10381 cggtacggag gaagtgccgc agtatgatgt tgcggaggcg gacaccgatg ccgaagccga
    10441 acgtttcgat gccgccgtca aagccacgcg caaagagttg gaacagctcc gcagcgcgat
    10501 tcccgaaaac gccccgaccg agttgggcgc gttcatctcg ctgcacctga tgctcttgac
    10561 cgatgttacc ttgtcgcgcg aacccgtcga tattttaagg gaacaaaaaa tcaacgccga
    10621 gtgggcattg aagcagcaga gcgacaaact cgccgcccaa ttcgacaata tggacgatgc
    10681 ctatttgcgc gaacgcaagc aggatatgct gcaagtcgtc cgccgcatcc acaacaacct
    10741 gatcgggcag ggcaacgagt tggaagttgc cgacaacctg tttgacgaaa ccgttctgat
    10801 tgcaaacgac ctttcgcccg ccgacacggt tttgtttaaa gagcagcgca ttgccgcctt
    10861 cgttaccgat gccggcggcc ccaccgggca tacggcgatt ttgggcagga gcttggacat
    10921 cccgtccgtc gtcgggctgc acaacgcgcg caaactgatt accgaaggcg aaacggtcat
    10981 tgtggacggt atcaacggcg tgttgattat cgcgccggat gagtcggtgt tgaacgaata
    11041 ccgccgccgt gcccgcgaat accgcagcca caaacgcgat ttgaacaagc tcaaaaaaac
    11101 cgccgccgcc accgccgacg gggtctgcat cgagcttgtg ggcaatatag aatccgccga
    11161 agacgtgaaa ccgctgcaca acctcggcgc agacggcatc gggctgttcc gcagcgagtt
    11221 tctttacctg aaccgcgata cgatgccgtc tgaagacgag cagtacgaag tgtacagcgc
    11281 gattgtcaaa aaaatgaaag gcaaaagcgt aacgatacgg acagtcgatt tgggtgtgga
    11341 caaaaacccg cgctggttcg ggaaaaacag cacgcccaac ggcagcctca accccgcgct
    11401 gggcatgacc ggcatccgcc tgtgccttgc cgaaccggtc atgttccgca cccagatgcg
    11461 cgccatcctc cgtgcggcgg tacacggccc cgtgcggatg atgtggccga tgattacctc
    11521 agtatccgaa gtgcgccagt gcctcatcca cctcgacacc gcgcaacgcc agcttgccga
    11581 acgcggcgat gccttcggta aagtcggcat cggctgtatg attgaaattc cgtctgccgc
    11641 gctgaccgtc ggcagtattt tgaaactggt cgatttcatc tccgtcggta ccaacgacct
    11701 gattcaatac atcttgtccg tcgatcgcgg cgacgacagc gtcagccacc tctaccagcc
    11761 cggccatccc gccgtgctga aaatgctgca acacgtcatc cgtaccgcca accgcatgga
    11821 caaagacgta tccgtatgcg gcgagatggc gggcgatacc gcgtttaccc gcgttttatt
    11881 gggtatgggg ctgcgccgtt tttccatgaa ccccaacaac atcctgcccg tcaaaaacat
    11941 cattctgcac agcaatgtcg gacagctcga aaacgagatt gccaaaatcg tccgttgcga
    12001 ggacgaggaa aaggcggaaa agctgatcaa acagatcaac agcgtgtctg tcgaggaaga
    12061 agccgacttc aaggggcgga aataaatacg gcaggtaaaa aatagaaata cttaacaatg
    12121 cccgcaatct gaaattttgc cattcttgca aaatagaaaa ccgaaacaga aacccaaaat
    12181 cggccattcc ctcaaaaaca gaaaaccaaa atcagaaacc taaaatccgt cattcccgcg
    12241 caggcgggaa tctaggtctg tcggcacgga aacttatcgg gtaaaacggt ttctttagat
    12301 tttacgttct gga
//
LOCUS       DAXHTR010000056        12303 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.056, whole genome shotgun sequence.
ACCESSION   DAXHTR010000056 DAXHTR010000000
VERSION     DAXHTR010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12303
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.056"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..218
                     /locus_tag="ACFNML_001046"
     CDS             <1..218
                     /locus_tag="ACFNML_001046"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392449.1"
                     /translation="RYWKMKEMKPVRYHIGDMPETSKQTASRHDDRAVGVDDDCFHDS
                     FGQFLFGCSAEATRTAPISVKRSCLFT"
     gene            326..1360
                     /gene="lpxK"
                     /locus_tag="ACFNML_001047"
     CDS             326..1360
                     /gene="lpxK"
                     /locus_tag="ACFNML_001047"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFC6392450.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLLRLDSVDAVVVSGGKADALFRPS
                     ENMFHSRIEAGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            1566..2147
                     /locus_tag="ACFNML_001048"
     CDS             1566..2147
                     /locus_tag="ACFNML_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HFC6392451.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAEAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPVGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PAFTEELRRIICGGKNPDAGCKQAGQVGKRHQK"
     gene            2216..2398
                     /locus_tag="ACFNML_001049"
     CDS             2216..2398
                     /locus_tag="ACFNML_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HFC6392452.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARPLGEEELEA"
     gene            2395..3156
                     /gene="kdsB"
                     /locus_tag="ACFNML_001050"
     CDS             2395..3156
                     /gene="kdsB"
                     /locus_tag="ACFNML_001050"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFC6392453.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAV
                     RVVVATDHPDIQTACQAHGVEVVMTSNRHESGTTRLAEASAALKLPPHLVVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYPRDAMRAGKREMPFETAVLRHIGIYAYRVGFLQRYTEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRIRAVFQTI"
     gene            3179..3496
                     /locus_tag="ACFNML_001051"
                     /pseudo
     CDS             3179..3496
                     /locus_tag="ACFNML_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3598..3798
                     /locus_tag="ACFNML_001052"
                     /pseudo
     CDS             3598..3798
                     /locus_tag="ACFNML_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            3955..4047
                     /locus_tag="ACFNML_001053"
     tRNA            3955..4047
                     /locus_tag="ACFNML_001053"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3989..3991,aa:Ser,seq:gct)
     gene            4350..5135
                     /gene="trpA"
                     /locus_tag="ACFNML_001054"
     CDS             4350..5135
                     /gene="trpA"
                     /locus_tag="ACFNML_001054"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFC6392454.1"
                     /translation="MSRIRQAFAALNGEKALIPYITVGDPDIGATLAIMHSMVENGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAMAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAVVGALVKELKDAVR"
     gene            5172..6044
                     /gene="accD"
                     /locus_tag="ACFNML_001055"
     CDS             5172..6044
                     /gene="accD"
                     /locus_tag="ACFNML_001055"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFC6392455.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEDGREEVAGNVKPIDPLKFKDSKKYPDRLGAA
                     RKLTGEDDALVVMKGMMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGVRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(6104..6715)
                     /locus_tag="ACFNML_001056"
     CDS             complement(6104..6715)
                     /locus_tag="ACFNML_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNP1-like family protein"
                     /protein_id="HFC6392456.1"
                     /translation="MRRTALLLLALAAGTSLATSFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIIPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGDTVNRRWIQPRNAEWKPIGGTLSRN
                     DALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSMKPHNK"
     gene            6824..7048
                     /locus_tag="ACFNML_001057"
     CDS             6824..7048
                     /locus_tag="ACFNML_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HFC6392457.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGVPG
                     DFEAFCRQTGHVLLDASEQDGVFTLVVQHK"
     gene            7411..8445
                     /gene="pyrC"
                     /locus_tag="ACFNML_001058"
     CDS             7411..8445
                     /gene="pyrC"
                     /locus_tag="ACFNML_001058"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFC6392458.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPALVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAIELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENTDTITLVKQSQTVPASIPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(8811..9236)
                     /gene="nusB"
                     /locus_tag="ACFNML_001059"
     CDS             complement(8811..9236)
                     /gene="nusB"
                     /locus_tag="ACFNML_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFC6392459.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAAEYIRQIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(9314..9790)
                     /gene="ribH"
                     /locus_tag="ACFNML_001060"
     CDS             complement(9314..9790)
                     /gene="ribH"
                     /locus_tag="ACFNML_001060"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFC6392460.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(9838..10161)
                     /locus_tag="ACFNML_001061"
     CDS             complement(9838..10161)
                     /locus_tag="ACFNML_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HFC6392461.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPQD"
     gene            10328..11047
                     /gene="rnc"
                     /locus_tag="ACFNML_001062"
     CDS             10328..11047
                     /gene="rnc"
                     /locus_tag="ACFNML_001062"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFC6392462.1"
                     /translation="MKDDVLKQQAHAAIQKRLGYAFRDISLLRQALTHRSHHAKHNER
                     FEFVGDSILNYTVARMLFDTFPKLTEGELSRLRASLVNEGVLAEMAAEMNVGDGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVVSCDLGELGFVCRAKGTSRKAAEQ
                     EAAKEALKWLEEKLPLKKKKK"
     gene            11095..12018
                     /gene="era"
                     /locus_tag="ACFNML_001063"
     CDS             11095..12018
                     /gene="era"
                     /locus_tag="ACFNML_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004286080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFC6392463.1"
                     /translation="MDIETFLAGERAAGGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDVV
                     VFVVEAMRFTDADRVVLKQLPKHTPVILVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         2819 a         2959 c         3594 g         2931 t
ORIGIN      
        1 aaagatactg gaaaatgaaa gagatgaaac ctgtccgtta tcatattggc gatatgcccg
       61 agacttcaaa acaaaccgcc tcccgtcatg acgacagggc agtgggtgtt gacgatgatt
      121 gttttcatga ttcctttggt caatttttgt ttgggtgttc ggcagaggca acccgaaccg
      181 cgccaatttc tgtaaagcgc agttgcttat ttacctgatt ggttcgctta tcggtttggt
      241 cttcgcgttg tttataggtg ggtctgtatc gggtacgcat gattaatgcc ccgggctgat
      301 tttgcttcga ggatttgtat cgaatatgcc gaattgtttc aaatttcata ctgttatcga
      361 acggcattgg caaaaacctt atccggtttt gtcttttctg cttaagccgc tctccgggct
      421 gtttgccaaa attgcggcaa aacggcggac ggatttttta tcgggaaaac ggcaaagcga
      481 aaagctgccc gtgcctgtgg tcgtggtcgg caatattcac gcgggtggga cggggaaaac
      541 gccgattgtt gccgcgctgg tgtcgggttt gcaggaaaag ggcgtcaagg tcggcatcat
      601 cagccggggc tacgggcgca agagcaaggc ggttcatgta ttgaatgctg agagccgagc
      661 ggaagatgcg ggcgatgagc ctttgctgct gttccgcaaa accggtgcgc cgacggcggt
      721 gggcagcagc cgtgcagagg caggcagggc gttgctggcg gcgcatcccg acatcggact
      781 gattgtggcg gacgacggtt tgcagcatta cgccctgcgg cgagatgtgg aaatcgcggt
      841 gtttccggcg gcggatacgg ggcgcacgga tttggattta ctgcccaacg gcagtttgcg
      901 cgaacctttg ttgcggctgg attcggtgga tgcggtcgtc gtcagcggcg gcaaggcgga
      961 tgcgctgttt aggccgtctg aaaatatgtt tcacagccgt atcgaagcgg gacggattta
     1021 ccgtttgaac aatccgtccg aaatactgga cacaggccgt ctgaaaaatc aaaccgtcgt
     1081 cgccgtggca ggtattgcca agccggcgcg ttttttcgat tcgttgcgga atatgggcat
     1141 taccgtgaag cgaaccgtcg cgctgcccga ccacgccgac atttcggcgg cagatttgcc
     1201 cgatgcggac gcggtcatta ttacggagaa agatgcggtc aaattttcag acggcatttg
     1261 caccgataat gtttgggtgt tgcccgtttg tgcgataatc gaacctgatt tggcggcgtt
     1321 tgtgttggag cggttggaag atgtaccgaa ggccgtctga aagcacggtt tgggcggagt
     1381 gattacggat ttgaataaga acgcctcgcg ccgtcattcc cgcgcaggcg ggaatctaag
     1441 tctcgaattt tcaggaatgc ctaggaggct tcagaaatcc caaatctccg gatttccact
     1501 tggacaggaa tgagaaaatc ggtcgtattt tttatctgca ttaatcattc gttaaagtat
     1561 tgaatattaa actgaaaacc ttgttattgc ccttcgccac gctggcattg tgcaccaacg
     1621 cttttgccgc cccgcctagc gacgcgtcgt tggcgcgttg gctggatacg cagaattttg
     1681 accgggatat agaaaaaaat atgattgagg gctttaatgc cggatttaaa ccgtatgcgg
     1741 acaaagccct tgccgaaatg ccggaagcga aaaaagatca ggcggcagaa gcctttaacc
     1801 gttatcgtga gaatgttttg aaagatttga ttacgcccga agtgaaacag gctgtccgta
     1861 atactttatt gaagaatgcc cgtgagatat acacgcaaga agaaattgac ggcatgattg
     1921 ccttttacgg ttcgcctgtc ggtcagtccg tcgttgccaa aaatccgcgc ttaatcaaga
     1981 aatcgatgag tgaaatagca gtatcttgga ctgcattgtc agggaaaatc gcgcgacatc
     2041 atctgcccgc gtttacggaa gagttgcggc gcatcatctg cggcggtaaa aatcccgatg
     2101 cgggctgtaa acaagccgga caggttggga aaaggcatca gaaataaatg atagccgtct
     2161 gaaatattga agagggcatc cgattgattg aaccatcaaa cccgaaagca accctatgga
     2221 aaaaaaattc ttagacatcc tcgtctgccc cgttaccaaa ggcaggctgg aatatcatca
     2281 ggacaaacag gaattgtgga gccgtcaggc gaagcttgcc tatccgatta aagacggcat
     2341 tccctatatg ctggaaaacg aagcgcgtcc gttgggcgaa gaggaactcg aagcatgacc
     2401 gaattcgtcg tattgattcc ggcgcggctg gattcgtcgc gcctgcccgg aaaagccttg
     2461 gcggacatcc acggcaaacc gatggtcgtg cgcgttgccg aacaggcggc aaaaagtaaa
     2521 gctgtgcgcg tcgtcgttgc caccgaccat cccgatattc agacggcctg tcaggcgcac
     2581 ggcgtcgaag tcgtcatgac ttcaaaccgg cacgaaagcg gcacgacgcg ccttgccgaa
     2641 gcctctgccg cgctgaagct gccgccgcat ctggtggttg tcaacgtaca gggcgacgag
     2701 ccgctgattg cacctgaact catcgaccgc accgccgaag tactcgtcga aaacaacgtc
     2761 caaatggcga ccgccgccca cgaattgcac gatttcgacg aattgatgaa tcccaatgcc
     2821 gtcaaagttg tcctcgacaa aaaccgcaac gccatctact tcagccgcgc cccgattccc
     2881 tatccgcgcg atgcgatgcg tgcaggaaaa cgcgaaatgc cgtttgaaac cgccgtcctg
     2941 cgccatatcg gcatctacgc ctaccgcgtc ggcttcctgc aacgctacac cgaaatgagc
     3001 gtttcgccgc tggaaaccat tgaatcgctg gagcaactgc gtgtcctgtg gcacggctac
     3061 ccgattgccg tcgaaaccgc caaagaagct cctgccgccg gcgtagatac gcaggaggat
     3121 ttggacagga ttcgcgctgt atttcagact atataaaata gattcaaagg gaaaagatat
     3181 gcagcaacat attgaaaaat ggcaacactt gagccgggaa gaacagaaaa tccttgctga
     3241 agtatggggt ctcgtgcaaa acgacgatca ggaggttcac tatgaaatgc tcaaattgaa
     3301 cgcacccgac gaagccagtg gcgaattttg gttcagaatg gcagaaacac tcagcaccct
     3361 gccgcccaac cgttccctcg accttagaat gaacggcggc aggctggcga ccgccgtatc
     3421 catcctttcc gtcatgattg aagacaatcc cgacataccg cagctttggg cgcaaaaaat
     3481 taccgcgctc aattatagtg gattaaattt aaaccagtac ggcgttgcct cgccttgtcg
     3541 tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccactatat
     3601 ttggcacacg gacacaaagc ccgtgccgac ggtttggcac aacagcccga caaagcggca
     3661 gaagccaacg aggaggaata cctgaccaaa gccctgtcgc aaaacctact gtcaacattg
     3721 gatgccgcgc ttgcacgttt tcctgaagac gcgtggtttc aggaaatcaa acaggatgca
     3781 caaaagcatt ttgcttgagg atgtggcagt caggaatatt tccattcagg aagaaaagaa
     3841 gtgcctgatt gggtataatc agggtaaatc ttattttatt tcaaaagatt aatatttgct
     3901 ttctgttttt ccttgacggt atcggaaaag ttgattatag ttacagcttc cttaggagta
     3961 atggctgaga ggctgaaggc acttccctgc taaggaagca tgtggggtca acctgcatcg
     4021 agggttcgaa tccctcttac tccgccaaga tgagaagacg ctgtgtttta cagcgtcttt
     4081 ttttacgcgc tttgttgcgg aagtgccgag ggctgtctgc cgtgcgccgc ctggatttgc
     4141 aatgtgcccg cccgtgcaaa atccgatttg accaacctga agggcaggtt tcggaagagc
     4201 ggggaatttt gataaacaaa aaactgcacg gcataatctt tcccggtttt cattttccgt
     4261 attcgcctat ccgtaccgca aatgctatac tgggaaaaat ttaccgattg tgttttacgg
     4321 catatttgcc gataggatgg aagagacaaa tgagcagaat ccggcaggct tttgccgctt
     4381 taaacggtga aaaggcattg attccttata ttacggttgg agacccggac attggggcga
     4441 ctttggcaat aatgcacagt atggtggaaa acggtgcgga tattttggag ttgggcgtgc
     4501 cgttttccga tccgatggcg gatggaccgg ttattcagcg tgcggcggag cgggcgttgg
     4561 caaacgggat ttcgctgcgc gatgtcttgg atgtcgtcag aaaattccgt gaaaccgaca
     4621 cgcaaacgcc ggttgttttg atgggatatt tgaaccctgt acataagatg ggttatcggg
     4681 agtttgctca ggaagccgca atggcgggtg tggacggcgt gttgacggtg gattcccctg
     4741 tcgaaaccat cgatccgctc tatcgcgagc tgaaggataa cggggtcgac tgtattttcc
     4801 tgattgcgcc gacgacgacg gaagaccgta ttaaaaccat tgccgagctg gcaggcggat
     4861 ttgtctatta tgtttcgctc aagggcgtaa cgggcgcggc aagtttggat acggatgagg
     4921 tttcgcgtaa aatagagtat ttgcatcagt atatcgatat tcccatcggt gtcggtttcg
     4981 gcatcagcaa tgcggaaagc gcgcgcaaaa tcggccgggt tgccgacgcg gtcattgtcg
     5041 gcagccggat tgtgaaagag attgaaaaca atgcgggcaa cgaggctgcg gtcgtcggtg
     5101 ctttggtcaa agagttgaag gatgccgtgc gctgattctg tcacgcattc taaatatttt
     5161 aggagttgtc catgagctgg ttagataaaa tcctgccacc caaaatcaaa aaccgcagca
     5221 aagacggttc ttccaatgtt cccgagggtt tgtggcacaa atgtccgtct tgttcggcaa
     5281 ccgtttattc gaccgagttg cagcagaaca atcaggtttg tccgaaatgc aaccaccaca
     5341 atccgttgtc ggcacgacaa cgcctgaacc tgcttttgga tgaggatggc agggaggaag
     5401 ttgccggaaa tgtcaaaccg atagatcctt tgaagtttaa agacagtaaa aaatatcccg
     5461 accgtttggg ggcggcacgc aagctgaccg gggaagatga cgcgctggtg gtgatgaagg
     5521 ggatgatgaa cggcctgccc gtcgtcgttg ctgcgtttga attccgcttt atcggcggtt
     5581 cgatgggttc ggttgtggga gagcgtttcg tacagggcgt ccgccgtgcg gttgccgaca
     5641 attgtccgtt cgtctgtgtg gcggcttccg gcggtgcgcg tatgcaggag ggtgtgaact
     5701 cgttgatgca gatgacgaaa accagtgccg cgctgcattt gctgacggaa aaacgcctgc
     5761 cgtttatatc ggtattgacc gatccgacta tgggcggcgt atccgccagc ttcgcatttt
     5821 tgggtgatgt tgtgcttgcc gaaccgaacg cgctgatcgg ttttgccggt ccgcgcgtga
     5881 ttgagcagac ggtgcgcgaa acgctgccgg aaggcttcca acgcgccgag ttcctgctgg
     5941 aaaaaggcgc aatcgaccag attgtcgacc gccgcgatat gaagcggcgc atcagtgatt
     6001 tgattacgct gttgtgccgt caggacaaag tttccgccgc ttgatggctg atgaatcgaa
     6061 taccgtctga aaccgatgtt tcagacggta tttttgtgtc tggttatttg ttgtgcggct
     6121 tcatcgacgg ggcatagcgt ccggcacgtt ctttcaggcg ttgtaccaaa cctttcgtgt
     6181 cggcgggtac accgccctcg cagaatgcct gatacaggac ggtgcgcagt gcgtcgttgc
     6241 ggcttaatgt accgcctatc ggtttccatt cggcgtttcg gggctgtatc cagcggcggt
     6301 tgaccgtgtc gccgtagccg aacactttat aggaggaagg tttgccgttg ccgaacctga
     6361 tggagaagcg ggcgcagaat atgccttcgg cagtcaggtt gtcgtagcct ttgtcggagc
     6421 ggatattgag aatgtagcgg atgctgccgt cgggcgcggg tataatttgc aggctgtcga
     6481 gcaggatttt cggctgtttg ccgtaatttt catccacata aatgtcaaac cagccgtccg
     6541 agtgcgtatc gggcagcggc ggcagtttgg cggtatgttc tttaaattcg cgggcggcgg
     6601 cttcttcggg cgtttcgcgg tagcgggtgt tgatcggcgt gtctttttgg ctgaagctgg
     6661 tggcaaggga cgtgccggcg gcgagtgcga gcagcaggag ggcggtgcgg cgcataagtt
     6721 tctccaaatt gaaaacggcg ttattttatg ggttggcaaa gggggctgca agcaactggg
     6781 gtataatctc cccccgattc ccatttttta aacggtacaa acgatgaaca gcgaaacttt
     6841 agacgtaacc ggattgaaat gtcccctgcc gattttgcgg gcgaaaaagg ctttggcgca
     6901 aatgcagcag ggcgacgtgt tgaccgttct ggcaaccgac ggcggcgtac cgggggattt
     6961 tgaggctttt tgccgccaaa ccggtcatgt gctgttggat gcttccgaac aggacggcgt
     7021 gttcacgctg gtcgtccaac acaaataaat gccgtctgaa atgcggatgt cccgcccgac
     7081 ggcgttgttt ttgaatatcg tatgtgccgc gtgccgtttc aaaaacaaac cgtccggcgt
     7141 gcagtgtcgg catttcgggc gtatcgccgc cgatttgttc cggaatggct gttaaccgcc
     7201 ttgccgtcgg caaagaagca aaacccaagc acaatcaaaa tctaaaggct gtgtttgaag
     7261 attccgttga tgcaacccaa tcagtcggac aaaatgcctt tcatccaatg gaaccggttt
     7321 ccgaccggac ggaataaccg gctttccccc ggcaaacgga tggaatcgac cgggtattca
     7381 aacgcagcca aaacctaaaa aggaacaacc atgcaaaccc tgaccattat ccgccccgac
     7441 gatatgcacc tgcacctgcg cgacggcgac gcgctcaaag ccgttgctcc ttataccgcc
     7501 cgccaaatgg ggcgcgccgt catcatgccc aacctcaaac cgcccgtcgt cagcgtagcc
     7561 gatgcgcttg cctacaaagc gcgtattatg gcggcgttgc ccgaaggcag cgcgtttgag
     7621 ccgctgatga cgctttattt gaccgacaac gccacgcctg cacttgtgcg cgaagccaaa
     7681 gccgccggca tcgtcgcctt caaactctac cctgccggcg cgaccaccaa ttccgattcc
     7741 ggcgtaaccg acctgttcaa gctcatcccc gtgttggaag aaatggcgaa acagggcatt
     7801 ttgttcctcg ttcacggcga agtaaccgac cccgaaatcg acatcttcga ccgcgaagcc
     7861 gcctttatcg ggcgcgtgat gaaacccgtt ttggcgcaag tgccgaatct taaagtcgtg
     7921 ttcgaacaca tcaccaccgc cgaagccgcc cgcctggttt tggaagcagg cgacaacgta
     7981 gccgccaccg ttaccccgca acacctcctg ctcaaccgca acgacctctt ggtcggcggc
     8041 gtgcgccccc atcatttctg cctgcccgta ctcaaacgcg aaacccaccg tcaggcattg
     8101 gttgccgccg ttaccggcga gaaggcgcat aaattcttcc tcggcaccga ctccgcgccg
     8161 cacgccaaat ccgccaaaga aaacgcctgc ggttgcgccg gtatgttcag cgcgatgacc
     8221 gccatcgagc tttatgccga agtgtttgaa aaagcaggcg cgttggacaa actcgaagcc
     8281 ttcgcctcga aaaacggcgc aaggttttac ggcattcctg aaaataccga cacgatcacc
     8341 ctcgtcaaac aaagccaaac cgtccccgca agtattccct acggcgacgg cgaacttgtc
     8401 ccgatgcgcg cgggcggcga aatcggctgg acggtacagt attgatgggg ctggaaacaa
     8461 aatgccgtct gaaaggtttt tcagacggca tttgtgttgt tgactgattg atacgtcaac
     8521 ggcgtttgag tttgttacgt ttcggttatt tccgataaat tcccacaatt ttcaaatttc
     8581 gccattccca cgaaggcagg aatccggaaa ttcgatgcga ccagagttta tcaaaaacgg
     8641 cagcaactca aaaaaccgga ttcccgcctg cgcgggaatg acgagattga agtttcagaa
     8701 tttatttgaa atacccaaaa ttcaaaaaac caaattccca cctgcgtggg aatgacgaaa
     8761 caaagaaagc agaaataagg acatagaact ttctttaaat ttgtgatgca tcaacggcgt
     8821 ttgggctcgt cgggacggat ttgggcggcg agtttgtcga ggatgccgtt gacgaatttg
     8881 tgcccgtccg tgccgccgaa ggttttggtt acttcgatgg cttcgttgat gatgacgggg
     8941 tagggcgttt cgggcatggc ggacagctcg tggcaggcgg tcagcaggac ggcgcgttcg
     9001 atggggttga ggtctttttc gtcgcggtcg agcagggggc ggatttgtcg gatgtactct
     9061 gccgcattgg tttgcgtgcc gaagaaaagt ttgttgaaca attcttcgtc tgccttggca
     9121 aagtcgggca tttcgcggat gtttttagca atctcgggcg cggcggtgcg gttgataagg
     9181 gattggtaaa cggcttgtac ggcaagctcg cgggaacggc ggcgggctgt tttcatgatt
     9241 tttccttgaa acggttgggc ggcacggtat gccgtctgaa acggaaaggg cgtatcggtg
     9301 tacgccctgt ttgttattct tcgtcttcaa actgttcttc gagcaggagg ttgacgaggt
     9361 tggcgcattc tacggcgact ttggcggcat ccgaggcttt ttcttcaatc cgttcgattg
     9421 cctgtgcgtc gttttccgtg gtcaggacgg cattggcaat cgggatgttg tagtcgagtg
     9481 cgacgcggct gacccctgct ccggattcgt tggaaaccag ctcgaaatgg taggtttcgc
     9541 cacggataac gacgccgatg gcaatcagtg cgtcaaattt ttcagaagag gcaaagttca
     9601 tcagcgcgat ggggatttca agcgcgccgg gtacggtggc gacggtaatg ttttcgtctg
     9661 ccacgcccaa ttcttggagg gtgcggcagc agactttgag catttcgctg ccgatttcgt
     9721 tggtgaagcg tgcctgtacg atgccgatgc ggaggtgttt gccgtcgagg ttgggggcga
     9781 tggtgttcat tgggtgtcct ttggtattcg gaggtttcgg aatgccgtct gaaggtttta
     9841 gtcttgcggc tgccagtcgg cgacggtttg gaatgtgccg tcttctgcca attcccacgc
     9901 gctgccttca ggttgggaga gcagtgcggc ggtttcaggg ttggttttgg tgatatcggc
     9961 gaggctgacg atgctgaagt tgtccgggtc gtcggtgtat tcgtcggtct cgtcgccgct
    10021 gaagaaacgc cagccgctgt cgttttcaaa aacgggggct tcgcggtaga ggaagccgac
    10081 gggctggttt tgtttggcga cggtgttggt ggcgatgcag cggtcgagtg ccgaggaaag
    10141 tgcttgtgca aatgcgttca ttgcgggaat acgttggggg aaaacttgcg gattttacca
    10201 cgattcttgc gttgtcggca gacggcggcg gtttggtggt acaatgtgcg ccgtttgcag
    10261 ccttaaggtg tttctgtatt tttggagtat ggaaacgcat tcgggctgtt ttttgcggaa
    10321 gacggtaatg aaagacgatg ttttgaaaca gcaggcacac gcggcgatac agaagagact
    10381 gggctacgcg ttccgcgata tttcgctttt gcggcaggct ttgacgcaca ggagccatca
    10441 tgcgaagcac aacgagcggt tcgagtttgt cggtgattcg attttgaatt atacggtggc
    10501 gcggatgctg tttgacacgt ttccgaagtt gaccgagggc gagttgtcgc ggttgcgggc
    10561 aagtctggtc aatgagggcg tgctggcgga aatggcggcg gaaatgaatg tcggcgacgg
    10621 tctgtacttg ggggcgggcg agttgaagag cggcggcttc agacggcctt cgatactggc
    10681 agacgcgatg gaggcgatgt ttgcggcggt cagcttcgat gccgatttca acacggcgga
    10741 aaaggtggtg cgccatttgt ttgccgaccg cgtccggtgt gccgatttcc aaaatcaggc
    10801 aaaagacggc aaaactgctt tgcaggaggc gttgcaggca cgccgctttg ctttgccgaa
    10861 ataccgtatc gaagagcaaa tcggccatgc caacgacagt atgtttgtgg tttcctgcga
    10921 tttgggcgaa ctgggtttcg tgtgccgtgc caaagggacg agccgcaagg cggcggaaca
    10981 ggaggcggcg aaagaggctt tgaaatggct ggaagagaag ctgccgctga agaagaaaaa
    11041 gaaatgaggc ggcgcgtgaa tatgccgtct gaacatatgg atacgaaagc aaatatggat
    11101 attgaaacct tccttgcagg ggaacgcgcc gccggcggat accgttgcgg cttcgtggcg
    11161 attgtcggcc gtccgaacgt gggcaaatca acgctgatga accatctcat cggtcagaag
    11221 atcagcatca ccagcaaaaa ggcgcagacg acgcgcaacc gcgtaacggg gatttatacc
    11281 gacgataccg cgcagttcgt gtttgtcgat acgcccggct ttcaaaccga ccaccgcaac
    11341 gcgctcaacg acaggctgaa tcaaaatgtt accgaggcgc tcggcggcgt ggatgtggtg
    11401 gttttcgtcg tggaggcgat gcgctttacc gatgccgacc gcgtcgtgtt gaaacaactg
    11461 cccaagcaca cgccggtcat tttagtggtc aacaaaatcg acaaggacaa ggcgaaagac
    11521 cgttacgcgc tggaggcgtt tgttgcccaa gtgcgcgccg aatttgaatt tgcggcggcg
    11581 gaggcggtca gcgcgaaaca cggattgcgg attgccaacc tgttggagct gattaagccg
    11641 tatctgcccg aaagcgtgcc gatgtatccc gaagatatgg ttacggacaa atcggcgcgt
    11701 tttttagcga tggaaatcgt gcgtgaaaaa ctcttccgct atttgggcga ggaattgcct
    11761 tatgcgatga atgtcgaagt ggagcagttt gaagaggaag acggtttgaa ccgcatctac
    11821 atcgccgttt tggtcgataa ggaaagccaa aaggcgattt taatcggcaa aggcggggag
    11881 cgtttgaaga agatttccac cgaagcgcgg ttggatatgg aaaaactgtt tgataccaaa
    11941 gtatttttga aggtctgggt caaagtcaaa tccggttggg cggacgacat ccgcttcctg
    12001 cgcgagctgg gtttgtagtt tttcttgctg aactttacgc aaatgccgtc cgaacaggtt
    12061 tcagacggca ttttgtttca atcgggaata tctttgttaa aaacgggttg atattatctg
    12121 tgcatattat agtggattaa caaaaaccag tacggcgttg gctcgcctta gctcaaagag
    12181 aacgattctc taaggtgctg aagcaccaag tgaatcgatt ccgtactatc tgtactgtct
    12241 gcggctttgc cgccttgtcc tgatttttgt taatccacta taaagaccgt cgggcatctg
    12301 cag
//
LOCUS       DAXHTR010000057        12260 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.057, whole genome shotgun sequence.
ACCESSION   DAXHTR010000057 DAXHTR010000000
VERSION     DAXHTR010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.057"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            248..985
                     /locus_tag="ACFNML_001064"
     CDS             248..985
                     /locus_tag="ACFNML_001064"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HFC6392464.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKTRVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALAPKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            987..1673
                     /locus_tag="ACFNML_001065"
     CDS             987..1673
                     /locus_tag="ACFNML_001065"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HFC6392465.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKAVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPVA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPVAIIRKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            1832..2695
                     /locus_tag="ACFNML_001066"
     CDS             1832..2695
                     /locus_tag="ACFNML_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6392466.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPVAWNEGAAK"
     gene            complement(2757..3326)
                     /locus_tag="ACFNML_001067"
     CDS             complement(2757..3326)
                     /locus_tag="ACFNML_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HFC6392467.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVAHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            3484..4344
                     /locus_tag="ACFNML_001068"
     CDS             3484..4344
                     /locus_tag="ACFNML_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFC6392468.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGRIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            4480..4818
                     /locus_tag="ACFNML_001069"
     CDS             4480..4818
                     /locus_tag="ACFNML_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392469.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRNNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
     gene            5186..5539
                     /locus_tag="ACFNML_001070"
     CDS             5186..5539
                     /locus_tag="ACFNML_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HFC6392470.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
     gene            5529..6395
                     /gene="atpB"
                     /locus_tag="ACFNML_001071"
     CDS             5529..6395
                     /gene="atpB"
                     /locus_tag="ACFNML_001071"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFC6392471.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            6452..6688
                     /gene="atpE"
                     /locus_tag="ACFNML_001072"
     CDS             6452..6688
                     /gene="atpE"
                     /locus_tag="ACFNML_001072"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFC6392472.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            6759..7229
                     /locus_tag="ACFNML_001073"
     CDS             6759..7229
                     /locus_tag="ACFNML_001073"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFC6392473.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            7234..7767
                     /locus_tag="ACFNML_001074"
     CDS             7234..7767
                     /locus_tag="ACFNML_001074"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFC6392474.1"
                     /translation="MAEFATIARPYAKALFGLAQEKNQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            7778..9325
                     /gene="atpA"
                     /locus_tag="ACFNML_001075"
     CDS             7778..9325
                     /gene="atpA"
                     /locus_tag="ACFNML_001075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFC6392475.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIHGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTAFPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            9350..10225
                     /gene="atpG"
                     /locus_tag="ACFNML_001076"
     CDS             9350..10225
                     /gene="atpG"
                     /locus_tag="ACFNML_001076"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFC6392476.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            10263..11660
                     /gene="atpD"
                     /locus_tag="ACFNML_001077"
     CDS             10263..11660
                     /gene="atpD"
                     /locus_tag="ACFNML_001077"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFC6392477.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            11671..12093
                     /locus_tag="ACFNML_001078"
     CDS             11671..12093
                     /locus_tag="ACFNML_001078"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFC6392478.1"
                     /translation="MSIMQVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
BASE COUNT         3012 a         3135 c         3161 g         2952 t
ORIGIN      
        1 gtttttcaat tatttgccgt tttggtgcga accgctgcct ttgcccgttt cagacggcat
       61 tgtccgaaat ggttgcccgc ttcctgcttt attgacaaaa aaatgctttc ccgataatat
      121 cctacgaaaa ttaacctgcc gatttgacac agcttgcggg cataacagct aaagcgttcc
      181 gacaatttca gctttatctt ccgcgcccgt tgtgtccgac atcgggcttt gttgtatgga
      241 aaagacaatg attattttgg acaaggtttc caagcattac caaacgcgcg acaagacccg
      301 ttttgccgcc gtcgagccga ccagcctcga aatccgcgac ggcgaaatct tcgggctgat
      361 gggttattcg ggtgcaggca aatccaccct gttgcgcctg attaacctgt tggaacgccc
      421 cgacagcggc aaggtcaacg tctgcggaca agagctgacc gcgcttgatg ccgccgcatt
      481 gcgtcaggcg cggcagaata tcggcatggt atttcaacag ttcaacctgt tgaacaaccg
      541 caccgtcgcc ggcaacgtcg ccttcccatt ggaaatcgcc ggatggccgt ctgaaaaaat
      601 caaaacgcgc gttgccgaat gcctcgaaat cgtcggactg accgaacgcg ccggccacta
      661 tcccgcccag ctttccggcg ggcagaaaca gcgtgtcggc atcgcccgcg cactcgcgcc
      721 caaaccccaa gtcatcctcg ccgacgaacc cacttccgcc ctcgaccccg ccaccacgcg
      781 cagcgtcttg gaatgtttgg aagacatcaa caaacgattc aacgtaacca tcgtcatcgt
      841 aacccacgaa atgagcgtca tcagacgact gtgcgaccgc gccgccctgt tggacaaagg
      901 caaagtcgtc gaaatcgtcg aagtacgcgg caaccaaatc cacgcccgat ccgacatcgg
      961 gcgcgaactg attcgggagg actgatatgg cagacttaac attccaacaa gccgtttcca
     1021 acatcatcgg tatgaaaggc gaaatcgtcc gcgcattggg cgaaaccttc gtgatggtcg
     1081 gcttgtccac cacattcgcc gtcgttttcg gcacgttgct gggcgtgttg ctcttcgtaa
     1141 cttccagccg ccaactgcat tacaacaagg cggtgaactt cctgctcgac aacctcgtca
     1201 acctcatgcg cgccttcccc ttcgtcattt tgatgattgc gatgataccc gccacacgcg
     1261 ccatcgtcgg cagcaccatc ggtccggttg ccgcctcgct ggtgttgagc gtgtcgggat
     1321 tgttttattt tgcccgtttg gtggaacaaa acctgcgcga agtccccaaa ggcgtgattg
     1381 aagccgccgc cgctatgggt gcgccgccgg ttgccatcat ccgcaaagtc ctcttaaacg
     1441 aagcgcgcgc gggcatggtt tccagcatta ccgtgcttgc catcgggctt ttgtcataca
     1501 gcgcggcggc agggatgata ggcggcggcg gcttgggcga cctcgccatc cgctacggct
     1561 actaccgcta ccaaaccgaa gtcatcgtct tcatcgtcgc cctcctcgtg ctgctggtca
     1621 tcctgattca aagcaccggc aacgcgttgg cgcggaaact cgacaaacgt tgaacccaaa
     1681 tgccgtctga acgccaaaac ccccaccgct atccgaaaaa tgctataaaa tccccctgtt
     1741 cgcggcaaat gccgtctgaa cgccgaatcc ggacggcagg actccctgcc cgtcattttt
     1801 gtttgaaact gccacaacat caggagaaaa tatgaaaacc ttcttcaaaa ccctttccgc
     1861 cgccgcactc gcgctcatcc tcgccgcctg cggcggtcaa aaagatagcg cgcccgccgc
     1921 atccgcttct gccgccgccg acaacggcgc ggcgaaaaaa gaaatcgtct tcggcacgac
     1981 cgtcggcgac ttcggcgata tggtcaaaga acaaatccaa cccgagctgg agaaaaaagg
     2041 ctacaccgtc aaactggtcg agtttaccga ctatgtgcgc ccgaatctgg cattggctga
     2101 gggcgagttg gacatcaacg tcttccaaca caaaccctat cttgacgact tcaaaaaaga
     2161 acacaatctg gacatcaccg aagtcttcca agtgccgacc gcgcctttgg gactgtaccc
     2221 gggcaagctg aaatcgctgg aagaagtcaa agacggcagc accgtatccg cgcccaacga
     2281 cccgtccaac ttcgcccgcg tcttggtgat gctcgacgaa ctgggttgga tcaaactcaa
     2341 agacggcatc aatccgctga ccgcatccaa agcggacatt gccgaaaacc tgaaaaacat
     2401 caaaatcgtc gagcttgaag ccgcgcaact gccgcgtagc cgcgccgacg tggattttgc
     2461 cgtcgtcaac ggcaactacg ccataagcag cggcatgaag ctgaccgaag ccctgttcca
     2521 agaaccgagc tttgcctatg tcaactggtc tgccgtcaaa accgccgaca aagacagcca
     2581 atggcttaaa gacgtaaccg aggcctataa ctccgacgcg ttcaaagcct acgcgcacaa
     2641 acgcttcgag ggctacaaat cccctgtcgc atggaatgaa ggcgcagcca aataaggcag
     2701 tcgtataaaa tgatgccgtc tgaaccgtat ccgtgttcag acggcatttt tgtcctttaa
     2761 tccgccattc cctgccattc cgccgaatcc ggcgtatcga ttccgaacag cgacaaagcg
     2821 tgtgcaacac tgtgcgccac tatgtcgtcc gccgtctgcg gtttgcggta catcgcagga
     2881 acagggggaa acaccacgcc gcccatttcc gttacccgct tcatattgtc caaatgggca
     2941 aggttcagcg gcgtttcgcg caccatcagc accagccgcc gcctttcctt caaaaccaca
     3001 tccgccgcac gcgtcaagag gttgtcgccg aagccgtgcg cgaccgaggc aagcgtccgc
     3061 atcgaacagg gggcgaccag catcccgtcc gttttaaacg taccgctggc aatgcacgcc
     3121 ccgatattgc cgatcggatg cacgaagtcc gcgagggcat atacctcgtc tctcgcataa
     3181 gccgtttccg aagcgcgcgc catctccgca cctttcgata ccacaaggtg cgtttcgata
     3241 tcttgcgcgc gtaaaagttc caaagccttc acgccgtatt ggaaaccgct cgccccactg
     3301 atgccgatta tcaaacgccg taccatcatc cgcctttccc ataaaaccgc ctgcaacggc
     3361 aaaccggcta ttatagtgaa aaaacagaaa tccgataaat acggatacaa attgtcggca
     3421 acacccaata tccgataaaa tatccgattt aacatcctat ctgaataggc acgggagggc
     3481 ggtatggcaa aagtaaaagg cggattgggg cgcggtttgg attcgctgct cgccaacggc
     3541 gcagacaaca gcagcggcga ccgattgacc acggttgcgg ttaaagatat ccggcccggc
     3601 cgctatcagg cgcgtgttca aatcgatgac gaagccttgc aggagctggc agattcgatt
     3661 aaggcgcaag gcgtgataca gcccgtcatc gtgcgcgaac acggactgtc ccgatacgaa
     3721 ctgattgcgg gggagcggcg ttggcgtgcc gcacagattg ccggcctgac cgaaatcccc
     3781 gccgttatca aaaccatcag cgacgaaacc gcgttggcaa tgggtttgat cgaaaacctc
     3841 cagcgcgaaa acctcaaccc catcgaagaa gcacaaggct tgaaacgcct tgccgacgag
     3901 ttcgggctga cccacgaaac catcgcccaa gccgtcggta aaagccgaag cgcgatttcc
     3961 aacagcctgc gccttttaag cctgcccgaa cccgtgcagg aaatgcttta ccaacgccgc
     4021 ctcgaaatgg ggcacgcccg cgcactgctg accctgcccg tcgtcgaaca gctcgaattg
     4081 gcgcaaaagg ctgtcaaaaa cggctggtcg gtgcgcgaag tcgaacgccg cagccaagcc
     4141 gcccttcaaa acaaacgtcc cgagcccaaa aaaactgccg ccgccgacat cggccgcctg
     4201 aatgatttgc tgactgaaaa actgggtgtc aacgccgaaa tcaaaaccgc caaccataaa
     4261 aaaggcagaa tcatcctgca tttcgacacg cccgaaacct tcgaccatat cttgaagcaa
     4321 ttgggcatag attaccgacc ttaattttgc gggatatacc gtctgaaata tagagaatag
     4381 ctttccagat tttaagtggg aaatataatt ctattgacat tttttctgct tcacgtaaga
     4441 atcgttttcc tgttttcatt tttaattttc gaggaaatta tgaacacacg catcatcgtt
     4501 tcggctgcgt tcgttgcgtt ggcattagca ggttgcggct caatcaataa tgtaaccgtt
     4561 tccgaccaga aacttcagga acgtgccgcg tttgccttgg gcgtcagccc aaatgccgta
     4621 aaaatcagca accgcaacaa tgaaggcata cgcatcaact ttaccgcaac tgtgggtaag
     4681 cgcgtgagcc aatgctatgt taccagtgta atcagcacaa tcggcgttac cacttccgat
     4741 gcaatttgtt tgggaggcgg aacgcacaaa ggcaaaagtc aatgcaatgc tttgcttaaa
     4801 gcggcaggcc gttgctaatc ctttattcgg aaaaggtcgt ctgaaaatat tttcagacga
     4861 ccttttattt attgagcaaa ttccccaaac tgccgacaat ccgaccgata aactgctaca
     4921 atttcgcgtt gattttttat tatttccacg taaagcaaac aaatcacgta agaacattaa
     4981 aagtgtaaag aaattgcgga caaactcctt aatatcctgt aattatatgt aatgtgtgtt
     5041 agaatgacac ctgtcgccgt tattcaggcg gcgggtgcgg cggattggga tacgttgttt
     5101 cacaaagatt aactgtcgct tgacgtaata aggcttctaa attatattag ccgcgcccca
     5161 aatccgaggc ggaaacattc cctttatgaa gcagattatc atcctacaat ccgccgtatt
     5221 gtccatatgc gccgcagttg cctttgctgt atggggtttt gccggattcc tttcggcggt
     5281 cggtggcggt ttgtcctacc tgcttcccac gtttgttgca gttttacttt taaaactttt
     5341 caggggaaac cccttcctgc aaagcaggat gttcgtcttc ggagagattt taaaagtagt
     5401 gctgtcgctg ttgtccatgc tcgccgtatt tgctgtatgg catcaatcgt tggtgttcgc
     5461 cccgtttctg atggggctgc tcggtgtcag ccatttggtt tttttagtat tgttgagagt
     5521 gaaagattat ggcaggtgaa accattaccg ctgccgacta catcaagcac cacttgcaga
     5581 gcttgaccag tttgtcggat gttactcagg gtcagggact gaaaaacatt gctgattttt
     5641 cgtttattaa ccttgatgcc gtcttttttg ccgtcctgtt gggcgtaatc ggcagcttcc
     5701 tgttgtggcg cggtgcgaaa aaagcaacgg caggcgttcc cggacgtttc caggctgccg
     5761 tggaaatctt gtttgagttt gtggacgata tgtgtaagag catcattcac aacgaaaagt
     5821 ctagaaaagc cgtcgcgccg ttgggtctga cgctgtttgt ctggattttt ctgatgaacg
     5881 cgatggatat gctgccggtc gatttgctgc cgatggtatg gcagggcatt accggcaacc
     5941 atcacgccct gctgcgcgtc gtaccgactg ccgatttgaa caccactttg gcactcgctg
     6001 tcggcgtgtt gctgatttgt atctattaca acatcaaaat caaaggattg ggtggctggt
     6061 ttcacgagtt gttcagcgcg ccgttcggtg caaaactcgc acctgcgaac tttctgttga
     6121 atttggtcga gtttctttct aaaaccgtat cccacggtat gcggttgttc ggcaatatgt
     6181 atgcgggcga attggtattc ttgctgatag ccttgcttgg cggtgcttgg gcggcttccg
     6241 gcagcgtcga agtcatggat ccgattctgt ttgtattcca cattattgcc ggtttggcat
     6301 gggcgatttt ccatattttg gtgattaccc tgcaggcgtt tattttcatg gcgttggcgt
     6361 tcgtctatat cggacaggca catgatgcgc actgattttc ctaagtagtt tgtttttgta
     6421 gtttaacctt tgtttcttta aggagtttaa aatgggtttg attgctatcg catgtggttt
     6481 gatcgttgca ttgggtgcat tgggtgcctc tatcggtatc gcaatggtcg gttctaaata
     6541 tttggagtct tctgcccgtc agcccgaact gatcggtccg ctgcaaacca aactgttcct
     6601 gattgccggt ttgattgatg ccgcattctt gattggtgtc gccattgcac tgctgttcgc
     6661 cttcgttaac ccgtttgcag gtgcataatc aggacggatt cgtccgttcc gtacaggcgc
     6721 agcctgtttg attaacccca gtacgaaggt taagtaacgt gaatatcaat gcaacattat
     6781 tcgctcaaat catcgtcttt ttcggtttgg tatggtttac catgaaattc gtgtggccgc
     6841 cgattgcaaa agctttggat gagcgtgccg caaaagtcgc cgaaggcttg gctgccgccg
     6901 agcgtggtaa aagcgatttc gagcaggctg aaaaaaaggt tgcagaactc ttggcagaag
     6961 ggcgtaatca ggtttccgaa atggttgcca acgccgaaaa acgtgccgcc aaaattgtcg
     7021 aagaagccaa agaacaggct tcttccgagg cggcgcgcat tgcagctcag gcaaaggccg
     7081 atgtggagca ggaattgttc cgcgcacgcg aatccctgcg cgagcaggtt gccgtgttgg
     7141 ctgtcaaagg tgccgaatct attttgcgca gcgaagtcga tgcttccaaa cacgcaaaac
     7201 tgctcgatac cctgaaacag gagctgtaat cttatggcag agttcgcaac gattgccaga
     7261 ccttatgcaa aggcattgtt cggtctggct caggaaaaaa accaaattga gtcttggttg
     7321 ggcggactgg aaaaacttgc ggcggttgtt caggaaggga aggtggcttc attgattgac
     7381 cgtcctgaaa cgaatgcttc agaaaaagca gatatcctca tcgatttggt cggtttgaaa
     7441 gacaaggagt tgaaaaactt tgttatcgtc ttggccgggc agaaacgttt gtcgatattg
     7501 ccggaagtat atgctcaata tcaagacttg accttatcat tcaaccatat caaatctgcc
     7561 gtcatttaca gtgcctatcc gttgaccgac aaacaggtcg gcgagttggc gcaaatgctg
     7621 aataagcgtt tcgacagcga gctgaaaatc tctgtcgaaa tcgaaccaga gctgattggc
     7681 ggcataaaag ttgaagtggg tgatcaggtt ttggatttgt ctgtacaagg caaattgagt
     7741 gccttgtaca cgactatgac gaattaggag agttttcatg cagcttaatc ctgctgaaat
     7801 tagcgatttg attaaagcca agatcgaaaa tctgtctgta aatgccgaag tgcgtacccg
     7861 tggtacggtg atttcggtaa ctgacggtat cgttcgcatc catggtttgt cagatgcaat
     7921 gcaaggtgag atgctcgaat tcccgggtaa cactttcggc ttggcgatga acctggagcg
     7981 cgactccgtc ggcgccgtag tgttgggcga gtacgaacat attaaagaag gcgacacggt
     8041 tacttgtacc ggtcgtatct tggaagtgcc ggtcggacgc gaactggtcg gacgcgtcgt
     8101 tgatgcattg ggtcgtccga ttgacggcaa aggcccgatt aatacaactt tgaccgcccc
     8161 tatcgaaaaa atcgcaccgg gcgtgattgc gcgcaaatcg gttgaccagc cgatgcaaac
     8221 cggtctgaag gcgattgact ctatggttcc tgtcggtcgc ggtcagcgtg agttgattat
     8281 tggcgaccgt cagacaggta aaacggccgt agcattggat gccatcgtca accaaaaagg
     8341 tacgggcgtt atctgtatct atgtcgctat cggtcaaaaa gcatcttcta ttgccaacgt
     8401 ggtccgcaaa ttggaagagc atggcgcgat ggaacacacc atcgtggttg ctgcaactgc
     8461 atctgaagcg gcggcattgc aatatatcgc accttactcc ggttgtacga tgggtgaatt
     8521 cttccgcgac cgcggcgaag atgccttgat tgtttatgac gatttgtcca aacaggctgt
     8581 ggcttaccgt caaatttccc tgcttttgcg ccgtccgccc ggccgcgaag cctatcccgg
     8641 cgatgtgttc tacctgcact cccgtctgtt ggaacgtgca gcacgtgtca atgaacacga
     8701 agtggagaaa ttgaccaacg gcgaagtaaa aggcaaaacc ggttctctga ccgcgtttcc
     8761 gattatcgaa acccaagcgg gcgacgtatc tgctttcgtt ccgactaacg tcatttcgat
     8821 taccgacggt cagattttct tggaaaccga cctcttcaac gccggtatcc gtcctgcaat
     8881 caatgccggt atttccgtat cccgcgtagg cggtgctgca caaaccaaag tgattaaaaa
     8941 gctgggtggc ggtatccgtt tggcgttggc gcaatatcgt gaattggcgg ctttctcgca
     9001 atttgcatcc gatttggatg aagctacgcg caaacaattg gagcatggcg aagtcgtaac
     9061 cgaactgatg aaacagaaac agttcagtac gttgaatacg gctgaaatgg ctttgaccct
     9121 ttgggcaatc aacaacggtt cgtattctga tgttccggtt gccaaagctc tggcttttga
     9181 atctgaattt ttgagctttg tccgtaccca acatccggaa gttttggaag ccgtcaatgc
     9241 ttcaggtgca atgtccgacg agagcgagaa aacgcttgaa gcagccatga aatccttcaa
     9301 atcttcttac gcctatcagg cataaggctt gaagtgaaag gagtctgaaa tggcagtagg
     9361 aaaagagatt ctcaccaaaa tccgcagtgt tcagaatacc caaaagatca ctaaagcgat
     9421 gcagatggtg tcaacctcta aaatgcggaa gactcaggaa cggatgcgtt tggcgcgtcc
     9481 gtatgccgaa aaagtgcgta tggtgatgag ccatcttgcg caaaccaata ccgatcatgg
     9541 tattccgtta ctggaatctc atcgggaaat cagacgtgtc ggttttattt tgattacgtc
     9601 tgataagggt ttgtgtggcg gtttgaacgc caacgtgctg aaaaagtttt tggcacaagt
     9661 tcaagagtat cggaatcaag gtattgaaga ggaaatcgta tgccttggca gtaaaggtct
     9721 gatggcgtgt cagagcattg gtctgaatgt ggttgccagt gccgtaaatt tgggcgatac
     9781 cccaaaaatg gaaatgctgc tcggaccttt gacagaactc ttccaacggt atgagaaaca
     9841 tgaaattgac agaatccatc tggtgtattc gggttttgtc aataccatgc gtcaagaacc
     9901 gagaatggaa gtattgctgc ctatcggtga gaacgtgatt ggcgattcag ctcccaaatc
     9961 accgttcagc tgggaatacc gctacgaacc gactgcactt gcagtgttgg aatatctggt
    10021 tcgccgctat ttagagtctg tggtttatca ggcgttgagc gacaatatgg catccgaaca
    10081 ggcagcccgc atggttgcca tgaaagctgc aacagacaat gcaggcaatg ccatcaaaga
    10141 gttgcgtttg gtatataaca aatcgcgtca agctgcgatt accacggaat tgtcagaaat
    10201 tgtagcaggt gcggcggctg tctgatgccg tctgaatcct gtacagaaat taggatacga
    10261 taatgagcca aggcaaaatc gtacaaatta tcggtgcggt tgttgacgtg gaatttccac
    10321 gcgacatgat tccgcgcgtt tacgacgctt tgaaattaga cgaaaacggt ctgactttgg
    10381 aagtccaaca gcttttgggc gacggcgtag tccgtaccat tgcgatgggc agctcggacg
    10441 gtttgaaacg cggcatgact gtgagcaata ccggtgcgcc cattactgtg ccggtaggta
    10501 aaggtacatt gggtcgtatt gtcgacgtat tgggtacgcc tgttgacgag gcaggtccga
    10561 tcgataccga caagagtcgt gccatccacc aagccgctcc taagtttgac gaactgtctt
    10621 ccacaaccga attgctcgaa acgggcatta aagtgattga cttgctgtgt ccgtttgcca
    10681 aaggcggtaa agtaggtctg ttcggcggtg ccggtgtggg taaaaccgtg aacatgatgg
    10741 aattgatcaa caacatcgcc aaagcgcaca gcggcttgtc cgtgttcgca ggcgtgggtg
    10801 agcgtacccg cgaaggtaac gacttctacc acgagatgaa agattccaac gtattggata
    10861 aagtggcaat ggtttacggt cagatgaacg aacctccggg caaccgtttg cgcgtcgcat
    10921 tgaccggttt gaccatggcg gaatacttcc gtgacgaaaa agacgaaaac ggcaaaggcc
    10981 gcgacgtatt gttcttcgtg gacaacatct accgttacac tctagctggt accgaagtat
    11041 ccgcattgtt gggccgtatg ccgtctgcag tgggctacca accgacattg gcagaagaaa
    11101 tgggtcgttt gcaggagcgt attacctcta cccaaaccgg ttctattact tctatccaag
    11161 ccgtatatgt acctgcggac gacttgaccg acccgtctcc ggcaacaacg ttcgcccact
    11221 tggacgcaac cgtcgtattg agccgcgata ttgcctcttt gggtatttac ccggcagtcg
    11281 atccgcttga ttccacttcg cgccaactgg atccgatggt cttgggtcaa gagcactacg
    11341 acgtggcgcg cggcgtacag tccaccctgc agaaatacaa agaattgcgc gacatcatcg
    11401 ccatcttggg tatggacgaa ttgtctgacg aagacaaact gaccgtaatg cgtgcgcgta
    11461 aaatccaacg cttcctgtcc caaccgttcc acgttgccga agtgtttaca ggttctccgg
    11521 gcaaatatgt cgccctgcgc gataccattg ccggcttcaa agccatcttg aacggcgaat
    11581 acgatcatct gcccgagcag gcattctata tggtcggcag cattgaagaa gcggttgaga
    11641 aagcgaaaac cttaaactga ggaggtcggc atgagcatca tgcaagttga ggtggtaagt
    11701 agcgagcaga aaatctattc aggcgaggca acatttatcg ttgttccgac tgtacagggc
    11761 gaactcggta tttatccgcg acacgagccg attatgagtt tggtgcggcc gggggctttg
    11821 cgtttgaccg ttccgggcga ggataaagag gttttggttg ctgtttccgg cggtgttttg
    11881 gaagtacagc ctgataaagt aactgtcttg gcggatgttg ccgtccgcag tgcagagatg
    11941 gatcaggcac gtgcggaaga ggcgaaaaaa gccgcagaag cgggcatttc ccaagctaaa
    12001 gacgataagg ctttggcgga ggcgcataaa gcattggctg ctgcgattgc acagcttaaa
    12061 actttggact atatccgttc gcacaagaaa taaacttgtt tggtttggaa aagcacgacc
    12121 gtttggtcgt gctttttatt ttccggattt aaattaaaga ctgtgtttgg atatggcgtt
    12181 ggcaggcata gcctgcttta aggtattcaa tagggcagcc tgccgcctaa ccgaaacata
    12241 tagcggatta acaaaaatca
//
LOCUS       DAXHTR010000058        12251 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.058, whole genome shotgun sequence.
ACCESSION   DAXHTR010000058 DAXHTR010000000
VERSION     DAXHTR010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.058"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(71..487)
                     /locus_tag="ACFNML_001079"
     CDS             complement(71..487)
                     /locus_tag="ACFNML_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392479.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            complement(484..675)
                     /locus_tag="ACFNML_001080"
     CDS             complement(484..675)
                     /locus_tag="ACFNML_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392480.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            complement(719..961)
                     /locus_tag="ACFNML_001081"
     CDS             complement(719..961)
                     /locus_tag="ACFNML_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392481.1"
                     /translation="MDTLKVNPFPNKIKEKCRLKTARMLFQTAFFVVGHQCPAICAAL
                     RLSRYGILVCIFRRNLPYIVLKYYTVVLYGFDFLEK"
     gene            1198..1365
                     /locus_tag="ACFNML_001082"
     CDS             1198..1365
                     /locus_tag="ACFNML_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392482.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            1638..1823
                     /locus_tag="ACFNML_001083"
     CDS             1638..1823
                     /locus_tag="ACFNML_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392483.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            2024..2224
                     /locus_tag="ACFNML_001084"
     CDS             2024..2224
                     /locus_tag="ACFNML_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392484.1"
                     /translation="MDSIKKAQEKYALKRVVKTVSFNSEKELDLIKFSENLDFSNWVK
                     SKIRGDMESEKFKKKAKKNIDK"
     gene            2374..2964
                     /locus_tag="ACFNML_001085"
     CDS             2374..2964
                     /locus_tag="ACFNML_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HFC6392485.1"
                     /translation="MNTQLIPTVSGQLDNQTQALVDAHDLHKFLGVETPFSKWIQRRI
                     EEYGFTQALDFIGVDKIVRTEAGFFGQRDKTVQGYYLSLDMAKELCMVERNDKGRQAR
                     RYFIEMEKQAKALPDAVLYRIDALEDAYFQAAPEMLALLRYRSMGLNLTEIGKLLDMN
                     PGAVSYRLKKLNDLGFLEYVPKPKMVVQQQSLGLEG"
     gene            2968..3183
                     /locus_tag="ACFNML_001086"
     CDS             2968..3183
                     /locus_tag="ACFNML_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392486.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(3344..3862)
                     /locus_tag="ACFNML_001087"
     CDS             complement(3344..3862)
                     /locus_tag="ACFNML_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0042262 - DNA protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFC6392487.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAQAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDAILNQKERFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(3972..4139)
                     /locus_tag="ACFNML_001088"
     CDS             complement(3972..4139)
                     /locus_tag="ACFNML_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392488.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(4132..4305)
                     /locus_tag="ACFNML_001089"
     CDS             complement(4132..4305)
                     /locus_tag="ACFNML_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392489.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATKFMVKRNREQEVAEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(4414..4680)
                     /locus_tag="ACFNML_001090"
     CDS             complement(4414..4680)
                     /locus_tag="ACFNML_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392490.1"
                     /translation="MQVLKKVDWKMFVARSFWRWVPVGLTVGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            complement(4770..5684)
                     /locus_tag="ACFNML_001091"
     CDS             complement(4770..5684)
                     /locus_tag="ACFNML_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFC6392491.1"
                     /translation="MKQINQALQQKLVEFKEKSGMNQTQLARGIDTSPASISMYLNGT
                     YADKGGNYETIETKIEAFLEMEDSKARREELVLGFVSTKTTRRIAEVMRDAHEGGETV
                     VIYGQAGLGKTQSVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGRLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKHG
                     ELVQLYSRVSVALNLGESLPDDELFEIAKAALPDADEETLLELVKHSNGNTRRMSKLM
                     RGAVRTANKNGIKMQAGIVKKYSSLIIR"
     gene            complement(5720..7696)
                     /locus_tag="ACFNML_001092"
     CDS             complement(5720..7696)
                     /locus_tag="ACFNML_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HFC6392492.1"
                     /translation="MKYMDLKSLLNFGLPNMPRTVIGLRKKAQRDGWQTRRRQGKGGG
                     VEYALPAEIRAAIMKRQSDELAEKMPKMLPQVRPGTAMSAQALAEAAKLLNEKQRSVA
                     NARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAESKLDAVTLGNLEKANDRSRTAKVG
                     ERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSAPKLAH
                     SYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPLIMQERGRKTGAAYKSLLPYVKRDW
                     GALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAESCVAV
                     SDALRIGVKHFGLPIIYYSDNGGGQTGKTIDHEITGITSRLGIRHETGIVGNPQGRGI
                     IERWWKDNLIEMARQYETFAGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQQKYLK
                     KLPSWSRFIADVVKCIDEYNNRPHGELPRHPDGGHYTPKAYREMRLEQDGIAPDMLSA
                     QELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASVVNVFDMD
                     GKFITKAQADGNTREAFPTARIDQLAENRRKGKIKRAENAIKLANAEVNPALEQAAAW
                     DELGHLGGNVIEAEYAVLPKTGTDDFVLFETDRS"
     gene            complement(7698..8027)
                     /locus_tag="ACFNML_001093"
     CDS             complement(7698..8027)
                     /locus_tag="ACFNML_001093"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6392493.1"
                     /translation="MYIIQVTVKAGIKACKVSNGFYHGGIMQANEIYKSLEEKNISAR
                     MLASALGVTNQSVSEVIRNGRGSKRIAEAIAKVIQKDLVEVFPHYKKTDCRDKKIAEL
                     KEMLGVG"
     gene            7993..8892
                     /locus_tag="ACFNML_001094"
     CDS             7993..8892
                     /locus_tag="ACFNML_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HFC6392494.1"
                     /translation="MPALTVTWMIYTKYLVYQIYLVIFCDVFMERIDLALNLVEERSR
                     LGYSRKNFAEQTGCSAESLRLYESGQVNIPADFLLAAVQLGVDIQFVFTGIHSANLNA
                     VSDDLNKKLDDAIGKSNVENQINGNVSNAVIASSGSVVHQINTQNHVIRTRVDSKPGK
                     EHITLEQASKLQQLVKQVAAAEEIAKRSPKSIRAIWASLNAHCKVPSYKLIALSDYDK
                     AETYLRKWLGRLSNTATSKNNDPDWRKKKYAYIKLNVKQLELEDWLKSYLEKNFSVES
                     LTELSDEDLQKTYAAVSTKKRKK"
     gene            9101..9316
                     /locus_tag="ACFNML_001095"
     CDS             9101..9316
                     /locus_tag="ACFNML_001095"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6392495.1"
                     /translation="MRIGNDLFVKRVQRIPGKLLVTSENPRYAPFEIDLSNTQDDIAI
                     IGRVEWYGRSIPHRKTSLCSATTAKSC"
     gene            9274..9864
                     /locus_tag="ACFNML_001096"
                     /pseudo
     CDS             9274..9864
                     /locus_tag="ACFNML_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            9968..10636
                     /gene="hda"
                     /locus_tag="ACFNML_001097"
     CDS             9968..10636
                     /gene="hda"
                     /locus_tag="ACFNML_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HFC6392496.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLQHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            10633..11301
                     /locus_tag="ACFNML_001098"
     CDS             10633..11301
                     /locus_tag="ACFNML_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6392497.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAKYIIPHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPVCHLFGITNIIGTQLETGSDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSRNDLPLLRLVSEPVAANPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         3301 a         3234 c         2477 g         3239 t
ORIGIN      
        1 aaaaatgccg tctgaaaacg agctttgcga gtttcgccaa aacgtttcag acgacctttt
       61 ttactacaat ctaatcactg tctttttcgc gccgttgctt ttccaaggtt tgcgctttac
      121 ctctgttgat tcctgcaaaa tatcccaaag ctaccgcccc gccgattttg acaatttcaa
      181 tagcgacgct gtccttatcg cgccataacg cagtaatcag cacggcggca atcagaaaag
      241 aaatagcaat cacggcattc agacggcgtt tgtgtacctc tttgaaaatc tcaccatgtt
      301 gctgttcacc ctgtttttgc gcttcgatgg tagccagtgc aatgcgttcg tgagattgga
      361 tttcgtcctt acggctttcc aactccttct gtttgactgc aatatcctgt ttttgaagtt
      421 ctaaaaactc ctgaagcatt ttctgctgct catcggccgg cagattctcg gatttttccc
      481 cgctcataaa tcaaccgcca ctaaggtttt gtaacggact ttgaatttgc cgtaaccccg
      541 agcccctatt ttcggcggca caaattccac ggacgcaata ttgcccgtct tacggtcttt
      601 acggacttct tccaaataat cggcagcatt gagcagccgt tcgcggactg ccgtatattc
      661 tttgacggta ttcatttttc aacctcgccc aaatagtgca aatattagca tatgggattt
      721 atttttcaag aaaatcaaat ccatataaaa caaccgtata atattttaat actatatatg
      781 gtaaatttct tctaaaaata cacactaaaa taccatatct tgataaccgc aacgccgcac
      841 aaatggcagg gcattgatgc ccgacaacaa aaaatgccgt ctgaaacagc attcgagctg
      901 ttttcagacg gcatttttct ttgattttgt tggggaaagg gttgactttt aacgtgtcca
      961 cgtttataat tcaattcatc ggcgtagtga gggccgatac gggaaaagcc ccaccgaagc
     1021 ggggctcaac tgggagaaag aagatgaata aacgcactat tcaaattctc atcttggtcg
     1081 cagttttcat gttggcaagt gctaatgcct actaaagact gaccagctgg gggcggtcgc
     1141 cgctgtcccc agtcccaatc ttacaaaata cggtttaaaa aatcaaggag tttgcctatg
     1201 gttgatgaaa aactggcaga aaaccgaaag cgttacgagc agaaacgggt gattaagaaa
     1261 gtctcgttca atgccgaaac ggaaaaagaa cttttggaat atgcccaaaa tatcgatttt
     1321 tcccaatggg ttaaatcaat catcaaagaa aaaatcaaaa aatagcttga ctaaattcta
     1381 gaaatactag ataatacgcc tatctgataa agcaaagccc cgaagcggca acttcggggg
     1441 cttatcggat agaaaggagg cttgtggaaa tgacaaaact ggcgaaagtt gtttttttca
     1501 tcattctgct tttaagctgt agtccggctt actaagcaag ttgattaaac acaaggggtg
     1561 gcagaacacc gcgccccttt cgccaacctt tctgcaagcc attttactac aacgatttta
     1621 aaaatcaagg attgcctatg cctgatgaaa aaacgctaaa agcaaaacaa gctgaatatc
     1681 agcgaaagta tgaccaaaag cggctgatga aaacagtttc tttccatttg gaaagagaga
     1741 aagagttggt ggattttatg caaaaagaaa ttcccgactt ttcaaactgg gttaaatcag
     1801 tcatgaagga aaaaatgaaa taagaaaatt ccgccgatgg gaaaacatcg gcggaacagg
     1861 tggaaagcag agcagcaact ctctttatgt agggcatgaa aaaaggccgt ctgaaacagg
     1921 ttttaaaccc catttcagac ggctttttgt tgtaatacat cttgattaaa atggttttta
     1981 gtattaccat aatcgtgttt taaaacctat ttaaaggaag attatggact cgattaaaaa
     2041 ggctcaagaa aaatacgcac tcaagcgagt agtgaaaaca gtatcgttca attccgaaaa
     2101 agaacttgat ttaattaaat tttctgaaaa tttagatttt tcaaactggg taaaatcgaa
     2161 aattagagga gatatggaat cggaaaaatt caaaaaaaaa gcaaaaaaga atattgacaa
     2221 atagaattac cataatttaa aatttccata tcttgtgcaa cacaagatat ggaaatcccc
     2281 gataagtgac agcttatcgg ggatagtgga aaagcaacgt tagagcgttc ttttccttgt
     2341 catctcaacc ttgaaatgaa agataatttt accatgaata cccaactcat cccaactgtt
     2401 tcaggtcaat tggacaatca aacacaagca ctggttgatg ctcatgacct gcataagttc
     2461 ttaggtgtgg aaacaccatt ttctaaatgg attcagcgtc gcattgaaga atacggattt
     2521 acacaagccc ttgattttat cggcgtggac aaaattgtcc gcaccgaagc aggtttcttt
     2581 ggacagcgtg ataaaactgt tcaggggtat tatttatccc tcgacatggc aaaagagctg
     2641 tgcatggtgg agcgcaacga caaaggacgc caagcccgcc gatattttat cgaaatggag
     2701 aaacaggcaa aagccctgcc tgatgccgtg ctataccgca tcgatgcatt ggaagacgcc
     2761 tatttccaag ccgcgcccga aatgctcgcg ctgctgcgct accgcagtat gggcttgaac
     2821 ctgaccgaaa taggcaaact cttggacatg aaccccggcg cggtttccta ccgtctcaaa
     2881 aaactcaacg atttgggttt cttggaatat gtccccaagc ccaaaatggt ggtgcaacaa
     2941 cagtctttgg gattggaggg ttaagccatg aataccacct ataccctgac tttcgaccaa
     3001 gacagcctca aagccctgaa tctgctggtg ttcacactca accatctgaa agttttggag
     3061 atggacatgg aaggaatcga agacggtttg tctgccgtga ttgaaaccgc ctccgagaaa
     3121 gccgacaagc tgtccgccgc tttctacaac gccgtgtacg agcaggaaga gaaagcagcc
     3181 tgaatctagg gcatgaaaaa aggccgtctg aaacaggttt taaaccccgt ttcagacgac
     3241 ctttaaatca tgggttaaag cctatttgaa ccggctaggc aagctcctgc tccgtaggct
     3301 caatcacgtt tcaacacact gaattgaaat caggctgttg attttataga atttgttcgc
     3361 tcggctcaat cacaaaatcc tccacgccgc tcacaatctt aatccccggc acttggccgt
     3421 ctgaaaaacg ctctttttga ttcaggatgg cgtctttgtc gatttccttt ttagtgcgga
     3481 caaactcggc aaaggcggat ttttccgaga gccacgccaa gacggcggcc acgcccgtta
     3541 ccttgacgga tggcggacgg atgcgccatt taatcaggcc ggtagtaaag tccacggttt
     3601 tggttttacc gttttccgtc agctcgtcct tatgtgcctc gcagtatgcg gccacacgtt
     3661 cggtcaggct catgatttcg gcacacatcg gcgcggcttg ggcggcatat tcttcttcga
     3721 tggccgcttt tttgtctccg gcttcggttt ccaggcgttt gacttcgcgc tgcaagtcgc
     3781 cgatttggcg gataaatgca gtaacttccg ttttgtcttg tgccgcttcg atggcgggct
     3841 gtttgattcg ggttttagcc attttctctt cctttcaggt tgggtttaac atttcggctt
     3901 actcaagcct tgttcgcgtt cgtgcttggc aagtgcttca actttcgcgc ggtttctcaa
     3961 aaacttaaac atcacacttc ctcccataaa gttatcgcct gagccaaagt ttccgcctcg
     4021 gcagtcttcc acatcccgtc cggcgcacgc gctgcaatca caaagccttc gccgtccttc
     4081 ttcatgacca tcagctcgcc acggtcttcg agccattcga ttaaatcttt ttcattcatc
     4141 tttgtttcct tccgttttca gacgaccttt gccgtcctta tcttcaaact gcgcctgata
     4201 ttcggcgact tcctgctcgc ggtttcgctt caccataaac ttcgtcgcac gcctgcggtg
     4261 ttgtccccaa gcctgccaat ccgtattacg tcgtccgaaa ctcatttcac acatcctttc
     4321 acaatcgcct tatcgccata tttcgcgcgg atttccttta ccgcccgtgc caaagcctct
     4381 tttttcgccg cagggctcaa taaaattggc ttatcactca taaacaatcc ctttcatttt
     4441 ttcctcgacg ctcattgact cgtattgccc ttcccatacc ttgacctcca aatccgcctg
     4501 tctttccatc gcctcaacct tcgtcggctc tttcgcaacc ggttcgggtt ctttcgcgca
     4561 ggaatgcaac gccattcccg ccacaaaaca ccacacgccc accgtcaaac caaccggcac
     4621 ccaacgccaa aaagagcgcg ccacaaacat cttccaatca actttcttca aaacttgcat
     4681 tttgcgtttt ccttttaaaa caacaactta ttaaaatcat agggtaaaaa aatatatagc
     4741 cctgtcagag acttaccgtt tcagacggcc tatcggataa tcagggagct gtatttctta
     4801 acgataccgg cctgcatctt gataccgttc ttgttcgccg tgcgtaccgc gccgcgcatc
     4861 aatttactca tccggcgcgt attgccgtta ctatgtttaa ccagttccaa gagcgtttct
     4921 tcgtccgcat caggcaaagc cgctttcgca atctcaaaga gttcgtcatc cggcaaagat
     4981 tcgcccaaat tcagcgcaac agacacgcgg ctataaagtt gcaccagctc gccatgctta
     5041 ccgcgcaaat tcgcaaccag tcggggcata ccgctcaaca ccaaaccgca gcccgtttca
     5101 tcatgcagtc ggcggataat ctcaagggcg cgcaagggca ggttttccgc ttcatcgacc
     5161 acaatcagac ggcccgaatc gcgcaggcgg tcagatacag actcaaacaa atcattcagg
     5221 ctgcccatcg ccgatacctt cgccgccgtc gccaacttgc gcatcagtac caaagccgta
     5281 aagctcggat tagcctcaat caagatagcc gcagggtttt tctcgcagta gtttttgacc
     5341 gactgcgtct tgcccaaacc cgcctgaccg tagatcacca ccgtttcgcc gccttcgtgc
     5401 gcatcgcgca tcacttccgc gattcggcgt gtcgtcttag tcgatacaaa tcccaacacc
     5461 agctcttcac gtcgcgcctt actgtcctcc atctccaaaa acgcctcgat tttcgtctcg
     5521 atggtttcat aattgccgcc tttatcggca taagtgccgt tcagatacat actgatggag
     5581 gccggcgaag tatcgatacc gcgtgccagt tgggtttggt tcattcccga tttttcttta
     5641 aattcaacca gtttttgttg caatgcttga ttaatttgtt tcattttttt agtcctttta
     5701 aaagaggttt aaaaccgttt taacttctat ccgtctcaaa tagcacaaaa tcgtccgtgc
     5761 ccgttttcgg caataccgca tactccgcct cgatgacgtt tccgcccaaa tgtcccagct
     5821 cgtcccaagc tgccgcctgt tccagtgccg gattgacttc cgcatttgcg agcttgattg
     5881 cattttccgc ccgcttgatt ttgccttttc ggcggttttc cgccagttgg tcgatacgcg
     5941 ccgtcgggaa agcctcgcgg gtattgccgt cggcctgcgc cttagtgata aacttgccgt
     6001 ccatatcaaa cacattgacc accgacgcat cgctcaaatc gtagctgacc cgtacctcgt
     6061 ccttgtgata ctccgccagc tcggttgaga aataagagtt gttgaacaaa tccagccaac
     6121 cgcgctgtac ctttcgcacc tcttgcggca taaacatcgt cgccagctct tgcgccgaca
     6181 acatatccgg cgcgataccg tcctgttcca gcctcatttc ccgataagcc ttcggcgtat
     6241 aatgcccgcc gtcaggatgt cggggcagct cgccgtgcgg gcggttgttg tattcgtcga
     6301 tacacttgac cacatccgcg ataaaacgcg accagctcgg cagttttttc aaatatttct
     6361 gttgttcctc cgttaaatcc ttgccttttt ccaaagcatt aaacgcactt tccatcttgc
     6421 ggtacatcag gttcttcgta ctgctgtcca ttcctgcgcc cgcaaacgtc tcatactggc
     6481 gcgccatctc aatcagattg tctttccacc atcgctcaat gatgccgcgt ccttgcgggt
     6541 tgcccacgat acccgtttca tgccggatac ccaatcggga cgtaatacct gtgatttcat
     6601 ggtctatcgt cttgccggtt tggccgccgc cgttatccga gtaatagata atcggcaaac
     6661 caaaatgctt gaccccgata cgcagagcgt ccgataccgc cacacaactt tcagcaagag
     6721 agaccgaaaa cccgaccaca aaccgcgtac aaccatcaat aatcaccgtc acttccggct
     6781 taaacggcct gccgtgtaca gggtgcgcca ccttcgcctt aaagctgtgg ccgtcgccga
     6841 tccaaacatc gttcggcttc aaagcccccc aatcacgttt cacataaggc agcagcgatt
     6901 tataagccgc ccccgttttc ctgccgcgct cctgcataat cagcggcagc ttgtcccaaa
     6961 cgcgacgcac catactcaaa ttaggcacat cattgaccgg catattttcc gcttcagccc
     7021 actgcacaaa tcggcggtaa ctgtgcgcca gcttcggagc tgaagggatg ttgtgaaact
     7081 gcataaacat cggcaaccaa ccgtagctct caatcggctt gaccgccttc gtcgtcttcg
     7141 gagccaaagc aaccaaccgc tccgtcgcgt tttccgcttt caaataagca gaaatccagc
     7201 cgtctaaagt acgttcgcca acctttgccg tccggctgcg gtcattggcc ttttccaagt
     7261 tcccaagcgt gaccgcgtcc aatttacttt ctgccagcaa gcccaaaaac tgagccaccg
     7321 cagcctttgc agagcaaccg tattggtatt taatccccaa taccgccgcc accaccgcac
     7381 atcgcgcatt cgccaccgac cgttgtttct cgttcaacag cttggccgct tcagccagtg
     7441 cctgagccga catcgccgtc cccggtctga cttggggcag cattttcggc atcttctccg
     7501 ccagctcgtc cgactgccgt ttcatgatgg ctgctcggat ttcggcaggg agggcatatt
     7561 caacaccacc acccttacct tgacgccttc tcgtttgcca accatccctt tgcgcttttt
     7621 ttctcagccc tattactgtt cttggcatat ttggcaaccc aaaattaagc aggcttttga
     7681 ggtccatata tttcatttca acccactccg agcatctctt tcaattcagc tatctttttg
     7741 tctcgacaat ctgttttctt gtaatgcgga aaaacttcaa ctaaatcttt ttgaatcact
     7801 ttcgcgattg cttccgcaat tcttttgctt cctcgtccat ttcttatgac ctcagaaaca
     7861 gattggttag ttacccctaa agccgaagcc aacattcttg ccgatatatt tttttcttct
     7921 aaggatttat agatttcgtt cgcttgcata atgcctccat gatagaatcc attacttacc
     7981 ttacaggctt taatgcctgc cttaactgtt acttggatga tatacacaaa atatttggta
     8041 taccaaatat atttggtaat attttgcgat gtttttatgg aacgcattga tttagcattg
     8101 aatttagttg aagagagaag ccgacttgga tactctcgaa agaattttgc tgagcaaact
     8161 ggatgcagtg cagaaagttt gagactgtat gaaagcgggc aggtcaacat tcctgcggac
     8221 tttttacttg ctgctgtcca attaggggtg gatattcaat ttgtctttac aggcattcat
     8281 tcggcaaatt taaatgctgt ttcagacgac cttaataaaa aattggacga cgctattggc
     8341 aaatcaaacg ttgagaatca gattaacggc aacgtcagta acgctgttat cgcctcatct
     8401 ggttcggttg ttcatcaaat taatacccaa aatcacgtca tcagaacccg tgtcgattca
     8461 aaacccggca aggaacatat aacgcttgag caagcttcaa agctgcaaca acttgttaaa
     8521 caagttgccg cagcagaaga gattgccaaa cggtcgccaa aatcaatccg tgcaatttgg
     8581 gctagtctga atgctcactg taaagttcca agttacaaac taatcgcatt gagcgattac
     8641 gacaaagcag aaacttatct cagaaaatgg ctgggtaggt tgagcaatac agcaacatca
     8701 aagaacaacg atcccgactg gcgaaaaaag aaatacgctt atatcaaatt aaatgtaaaa
     8761 cagcttgagc ttgaagattg gttgaaaagt tacctcgaaa aaaatttctc agtagaaagc
     8821 ctgactgaat taagtgatga agatttgcaa aaaacatatg cagcagtatc gactaaaaaa
     8881 cggaaaaaat aggcttaaac atgaaactcc gcctttaaat tcaagtttca tgtttagctt
     8941 atggtcttca gatgctttgt ttttttagat gtatgatatt aaaagatttt ttcaaacatg
     9001 gaactattat acaagttcca tgttcagttt ctagttaaag ccttttataa acattctaat
     9061 caaccacgcc gaaaccgagc cgcgcgacgg cctatatgtc ttgcgtatag gcaatgacct
     9121 ctttgtcaaa cgtgtgcaac gtataccggg caaactgctt gtaacgtctg aaaatccacg
     9181 ttacgcaccg tttgaaatag atttgagtaa tacccaagac gacatcgcca tcataggccg
     9241 tgttgagtgg tatggccgga gtattcccca caggaaaacg tcgctctgtt ccgccacaac
     9301 ggcgaaatcc tgttgagcgg catcaacatc aaactcaaaa acggcaactc cctactgata
     9361 cgcggcccga gcggttgcgg caaaacttcg ttgctgcgcg cgctggcggg gctttggccg
     9421 ttcggcagca gcggcaaagt cagccatccg ccgcatcaag acatcctctt cctgccgcaa
     9481 cgtccgtaca cggcacaagg cagtctgcgc gatgcgattt gttaccctgc catcgacaaa
     9541 caacatccgg aactgatcga agccatgaat acctgccgat taggttatct ggttgataaa
     9601 ttagataaaa ccgatgactg gcaacacaaa ctctccccag gcgaactgca acgtgttgcc
     9661 ttcgtccgcg ccctactctc gcagcccaaa gtcatcttgc tcgatgaagc taccgccgct
     9721 ttggacgaac cgaccgaagc catgctctac cgcgccttaa agcaaaaact gcccgacagc
     9781 atcatcatca gtatcggcca ccgcagcacg ctcaacgcgt tccacaatat gcacctcgat
     9841 gtcggcaacg ttgcctgcgg ctgaacgatg ccgtctgaag ccgttttcag acggcatcgt
     9901 caaattggaa aggcgcgcga aaacaacgta caataaacct atctgccaac ctctgaaatg
     9961 ctgcaccgtg aaccagctta tttttgattt tgccgcacac gactatccaa gtttcgacaa
    10021 attcctcggc acggaaaatg cggagctggt ctatgtcctc caacacaaac acggacagtt
    10081 tatctatgtc tggggtgaag aaggcgcggg aaaaagccac cttttgcagg catgggtcgc
    10141 acaggcactc gaagccggca aaaacgccgc ctatatcgat gccgcctcca tgccgctgac
    10201 cgatgccgcg ttcgaggcgg aataccttgc cgtcgatcaa gtcgaaaaac tgggcaacga
    10261 agaacaagcc ctgctttttt ccatcttcaa ccgcttccgc aacagcggca aaggcttttt
    10321 gctgctcggt tcggaataca cgccccagca gcttgtcatc cgcgaagatt tgcggacgcg
    10381 tatggcatac tgcctcgttt acgaagttaa gcccctaacc gaccaagaaa aaatcgacgc
    10441 gctcgccagt atggcggcgg cacgccaagt aaccgttgat tccgaaattt tcgaatacct
    10501 gctcaaacac tggcggcgcg atatggacag cctgatgatg atgctcgaca cgctggacaa
    10561 ctacgccgtc acgatgggca aacgcatcac cctgccgctt ttgcgccagc ttttgaaaca
    10621 acaggaaacc caatgaaaaa cctcgccatc ttcgacctcg acaacaccct catcaacacc
    10681 gattccgacc acgcctggcc gcaatacctc atcaaaaaag gcttggttga cgctgccgaa
    10741 accgaagcgc aaaacgaaaa attctaccgc gactaccaaa acggctgcct cgacatcgac
    10801 gcattcctca aattccacct cgccccgctc gcccgctaca gcaaagaaga gctggcggaa
    10861 tttcaccgcg aatttatggc gaaatacatc atcccccaca tctcgcctat gcagcgtatg
    10921 ctggtgcaga gccaccaaat ggcaggcgac gaaaccctcg tcatctcctc aaccaacgag
    10981 ttcatcatca cccccgtctg ccaccttttc ggcatcacca acatcatcgg cacacaactc
    11041 gaaaccggtt ctgacggccg ctacaccggc aattacatcg gcacgcccag cctcaaagaa
    11101 ggcaaaatta cccgcctgaa ccaatggctt gccgaacgcg gcgaaacgct ccaaagctac
    11161 ggcaaaacct atttttacag cgactccaga aacgacctgc cgctgctgcg cctcgtcagc
    11221 gaacccgtcg ccgccaaccc cgatgccgag ctggaaaaag aagccaaaga aaaaggctgg
    11281 ccggttttga atttcaaatg acgcaatgcc gtctgaagcc cgattcggcg ttcagacggc
    11341 attggggctg aaatcccaaa aactcaaagc cacaggaatt tatccggaaa aacaaaaaac
    11401 acaggaattc atcgggaaaa acaacaatct ttccaccgtc attcccacga aagtgggaat
    11461 cttgatccgt ccgcacggaa acttatattt cgtcatttgc acccgacata attcaatact
    11521 ttattttgca ataaattaac ggatgctgac aaattagatt ggattaatta ccctattatc
    11581 catattgggt tatcgggttg ctaattgagc agagcgtagc aaaccataaa gtgagtgttt
    11641 ccacccactc tgcatttact atgaagtggt tattaaatat gataatgaga cctattaaat
    11701 ttagtatggt aaatacgtta ttatttattg ttatatgtag ttcatttttt gatctgctcg
    11761 ttcaattatg tacaatttta tttcatagcc aaaaaatata ctttattaca ttatttttat
    11821 tatttatttt taattttgtt acaaaatcta tctatatggc aattatttat cctattttat
    11881 atttttttac gataaaaaaa tattatcctt actctaggaa agtgataatt ctattatcat
    11941 tagcattatc tatatatttt agttttatgg acttttactt tttttccata tattcagata
    12001 accttagcta tgaaacggag cctttacatt tatacatccc tattattatt aattttttct
    12061 cacttttagt ttctaatttt attttatctt ttatcaacaa gtaaatgtat atacaaacta
    12121 caatccaaaa gatcctaagc aaaccaccaa tcccacacca cccttaccgg ttcaaatccg
    12181 cacaaaaacc cacatcccgt cattcccacg aaagtgggaa tctaggacgt atagtggatt
    12241 aacaaaaatc a
//
LOCUS       DAXHTR010000059        12155 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.059, whole genome shotgun sequence.
ACCESSION   DAXHTR010000059 DAXHTR010000000
VERSION     DAXHTR010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12155
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.059"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            160..1212
                     /gene="bioB"
                     /locus_tag="ACFNML_001099"
     CDS             160..1212
                     /gene="bioB"
                     /locus_tag="ACFNML_001099"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFC6392498.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLDLIYQAA
                     EIHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLADAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(1359..1925)
                     /locus_tag="ACFNML_001100"
     CDS             complement(1359..1925)
                     /locus_tag="ACFNML_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6392499.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITNKQGKFIS
                     GTIIPITQADDKIPKIDPEKPLSGGLFI"
     gene            complement(2049..2288)
                     /locus_tag="ACFNML_001101"
     CDS             complement(2049..2288)
                     /locus_tag="ACFNML_001101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392500.1"
                     /translation="MGGCTFFRRTAFVKQRAFEVADGNVRVLQGRFDVFQNIGIGQVI
                     DRIIAAEHNVAHADNGNGIGCLFVRRILSVHIAGI"
     gene            2275..2508
                     /locus_tag="ACFNML_001102"
     CDS             2275..2508
                     /locus_tag="ACFNML_001102"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HFC6392501.1"
                     /translation="MQPPKICYAFRTPSAYGQYLADAGFDYLSFANNHSNDFGAQGIT
                     ATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            complement(2571..4430)
                     /gene="ilvD"
                     /locus_tag="ACFNML_001103"
     CDS             complement(2571..4430)
                     /gene="ilvD"
                     /locus_tag="ACFNML_001103"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFC6392502.1"
                     /translation="MPDYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQNDETVLPHSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDIAQIER"
     gene            complement(4602..6371)
                     /gene="cysI"
                     /locus_tag="ACFNML_001104"
     CDS             complement(4602..6371)
                     /gene="cysI"
                     /locus_tag="ACFNML_001104"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFC6392503.1"
                     /translation="MTVQTKTKGLEWQEKPLSNNERLKTESNFLRGTILDDLKDQLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSYYRSIRLTNRQTFQFHGVPKTKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESALHQQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGGGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDDLDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVG
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDASKAVAIARA"
     gene            complement(6398..8212)
                     /locus_tag="ACFNML_001105"
     CDS             complement(6398..8212)
                     /locus_tag="ACFNML_001105"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HFC6392504.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKAADSLKAAGIQVRLAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCQAGKDFDRRFEELGAKRLLERVDADLDFTASANAWTDNIAALLKEEAAKNRATP
                     APQAMPPADLQTVPESRYCKADPFPAALLANQKITARQSDKDVRHIELDLSGSDLSYQ
                     AGDALGVWFDNNPALVGEILDLLGIDPATEIQAGGKMMPVARALSSHFELTQNTPAFV
                     KGYAAFAHYEELDKIIADNAVLQDFVQNTPIVDVLHRFPASLTAEQFIRLLRPLAPRL
                     YSISSAQAEVGDEVHLTVGVVRFEHEGRARTGGASGFLADCVEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKEGFLHKYDFAWSRDQEEKIYVQHKIREQAAELWQWLQEGAYVYVCGDAAKMAKD
                     VEAALLDVIIQAGHLNRDDAEEYLNTLREEKRYQRDVY"
     gene            complement(8209..9495)
                     /locus_tag="ACFNML_001106"
     CDS             complement(8209..9495)
                     /locus_tag="ACFNML_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HFC6392505.1"
                     /translation="MTAQHQAPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDAHFVPVSALLGDNIVYPGGNMPWYKGEPLLSIL
                     ETLPGADEVSRTADDFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDESTYGTVAAGMIL"
     gene            complement(9534..9746)
                     /locus_tag="ACFNML_001107"
                     /pseudo
     CDS             complement(9534..9746)
                     /locus_tag="ACFNML_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase"
     gene            complement(9780..10520)
                     /locus_tag="ACFNML_001108"
     CDS             complement(9780..10520)
                     /locus_tag="ACFNML_001108"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HFC6392506.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRDIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            complement(10531..11982)
                     /gene="cysG"
                     /locus_tag="ACFNML_001109"
     CDS             complement(10531..11982)
                     /gene="cysG"
                     /locus_tag="ACFNML_001109"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFC6392507.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGAVAARKISLLLKAGAEVRVAAKH
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFHLAESCQKPVNV
                     VDDRDHCSFIFPSVIDRNPVQIAVSSSGSAPVLARLLRERLEALLPPSLGDMAEISGR
                     WRDAVKGKLKSVTERRRFWEKQFNGRFAALVKNRQNTLAERELAGQLEQSRQNDQGGS
                     VSLVGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGD
                     RTPQEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAA
                     VGATAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALI
                     AERLQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLH
                     EKLAWFGENGEGENRVGQTYPALGGLNAGQRAA"
BASE COUNT         2387 a         3281 c         3539 g         2948 t
ORIGIN      
        1 cgcggcacaa aggcggaaaa accgtttgcc ccgtattttc aaacgcgtta cacttgccgc
       61 cgctgttttc agccatttga ttacccgcaa ccgccgtcat tgcgccggcg gtttgcctgt
      121 cagcgtcatt gcgccgctgt aaatacgaaa gaacacatta tgaccgtatc ccccgtcgcc
      181 ttgcgccgta agaccgagtg caagcctcat cccaccgcgc gctattggaa aaaatgcgat
      241 gtcgaagccc tgttcggact tcccttcctc gacctcattt accaagccgc cgaaatccac
      301 cgccaaaatt tcaacccgcg cgaaatccag ctttccacgc tgttgtccat caaaaccggc
      361 ggctgtcccg aagattgcgc ctactgtccg caatcggcgc accacaacac caatctgggc
      421 aaagaacaga tgatggatgt ggatgaaatc gtcgaaaaag ccaaaatcgc caaatcgcgc
      481 ggcgcaagcc ggttttgcat gggcgcggcg tggcgcggcc ccaaacccaa agacgtggag
      541 acggtttccg caatcatcaa agccgtcaag ggcttgggta tggaaacctg cggcacgttc
      601 ggtatgctcg aagaaggtat ggcggaagac ttgaaagagg cgggcttgga ttattacaac
      661 cacaacctcg acaccgaccc cgaccgctac aacgacatca tccatacacg ccggcatgaa
      721 gaccgtatgg acaccttggg caaagtccgc aacgccggtt tgaaagtctg ctgcggcggc
      781 atcgtcggga tgaacgaaac ccgcgccgaa cgcgcagggc tgattgccag cctcgccaac
      841 ctcgacccgc agcccgaaag cgtgccgatt aaccggttgg tcaaagtgga aggtacgccg
      901 cttgccgatg ccgaggattt ggactggacg gaatttgtcc gcaccatcgc cgtggcgcgg
      961 attacgatgc cgcaaagtta tgtccgactg tcggcagggc gcagcaatat gccggaagcg
     1021 atgcaggcga tgtgctttat ggcgggcgcg aactcgattt tttacggcga caagctgctg
     1081 accacgggca atccggatga agacggcgac agaatcctga tggaaaagct caacctgtat
     1141 cccttacagt ttgagctgga aggcgaggtc gccgaagtgg aaaaagcctc tgggattaaa
     1201 gtggattatt gacgattgca aaaatgccgt ctgaaacccg aaaaaaggct ttcagacgac
     1261 atttgttcgg acggtatttc caatatcttt ttaccggcgc gtgatgttgc cgtcgggcga
     1321 gacatccagc ccgttctcgt tggggaagtc gctgcggttc agataaataa tccgcccgat
     1381 aacggttttt cagggtcgat tttggggatt ttatcgtcgg cttgggtaat agggatgata
     1441 gttccggaaa taaatttacc ctgtttgttg gtgataattt tgaaaatcgg cgcaatgccg
     1501 ctgatgccgg aggtgttgat tgcgccgtag gtggcaaagt tgccgccgct gtaagagatg
     1561 aagcggtcgc ggtaaagttc gacggcgcgt gtaacgtgcg gcccctgccc gaatacgaca
     1621 tccgcgccgg aatcgacggc aagccgtgca aactcgacga cgttgcccct gttttcccca
     1681 tagaagattt cggtatcgaa cggcaggtgt tccgcctgtt tcccttccgc gccgccgtgg
     1741 aacattacaa tgacgatgtc ggctttctgt ttggttttgg taatccgttt tctaactttg
     1801 gcataatcgt tcagtttgac ggcggcaagg ttgggggcga aggagacgaa gccggatctt
     1861 acgccgtttt tcttcaggat ggcggtttca aacctgtttt cgatgcccga atatttgatg
     1921 ttcaattcgt caaggttcgc cctttatgcc gtttccgcac cgcaaaccgc cggggtcaag
     1981 ccctcggcgc atcctgccgg aacggaatcc ccgtgctgcc gattgacggt tcaaaccccg
     2041 cgcccgtttc aaataccggc gatgtggacg gacaggatgc gcctgacgaa aagacagccg
     2101 ataccgtttc cattatcggc gtgggcgaca ttatgctcgg cagcaattat ccggtcgatt
     2161 acctgcccga taccaatatt ctgaaaaacg tcgaatctgc cttgcaagac gcggacatta
     2221 ccgtcggcaa cctcgaaggc acgctgtttg acgaaggcgg tacgccgaaa aaatgtgcaa
     2281 ccccccaaaa tatgctatgc attccgaacg ccctccgcat acgggcaata ccttgccgac
     2341 gcgggattcg actacctcag cttcgccaac aaccacagca acgacttcgg cgcgcaaggc
     2401 atcacggcaa cggcggcgag cggcagctct tttacatact cgatcgcgct aaagccgctg
     2461 ccgataacga ggccaaaatt gcggaaaata ccgccatcgc ccagataaat ttgtccatca
     2521 tcagaccttt actgttcaga cgagacagca tttgccgcac gttttggggc ttatctttcg
     2581 atttgcgcta tgtcgcgcac cgcgcctttg tcggcggaag tcgccatcgc gccgtaagct
     2641 cttaatgcgg cggagacgta gcggtcgcgg tttttaggct tccatgcttt gctgccgcgc
     2701 gcttccattt cggcacggcg tgcggcaagc tcttcatcgg aaatggcaag gtggatgctg
     2761 cggttgggga tgtcgatttc gacggtatcg ccttcgtgta ccaaaccgat cgcgccacct
     2821 tccgccgctt cgggcgaggc gtgtccgatg gacaaacctg atgtgccgcc ggagaagcgt
     2881 ccgtcggtta agagagcgca ggctttgccg aggcctttag atttcaggta ggaagtcgga
     2941 tacagcattt cctgcatgcc cgggccgcct ttcgggcctt cgtagcggat gatgacgatg
     3001 tcgccggcga cgatttggtt gcccaaaatg ccttctactg cgtcttcttg gctttcaaac
     3061 acgcgggcgc ggccggtgaa tttgaggatg ctctcgtcca cgcctgcggt ttttaccacg
     3121 cagccgcgct cggcgatgtt gccgaacaag accgccaaac cgccgtcttg cgagtaggcg
     3181 tgtgccacgt cgcggataca gcctttttcg cggtcgaggt cgagggtttt ccacatacgg
     3241 ttttgcgaga acgcttgggt ggtgcgtacg ccgccgggcg cggctttgaa gcgttcgatg
     3301 gcgcgggtgt tttcagggtt ggttacgtcc catttttcaa tcgcgtcttt cagcgtcggt
     3361 gcgtggatag tgtgcacgtc ggtgtgcagc ttgcccgctt tgtccaattc tttcaggatg
     3421 gcgaaaatgc cgccggcgcg gtgcacgtct tccatatagt agtcgtggtt gttgggtgcg
     3481 gttttgcaga tgcagggcac aacgcggctt aagcggtcaa tgtcggccat tttgaaatcg
     3541 acaccggctt cgttggcaac ggccaacagg tgcaaaatgg tattggtgct gccgcccatc
     3601 gcaatatcca tcgtcatagc gttttcaaac gcttttttgg tggcaatgct gtgcggcagc
     3661 acggtttcgt cgttttgttc gtaataacgt ttggtgattt cgacaatcat acgtcctgct
     3721 tcgaggaaca attctttacg gcctgcgtgg gtcgccaaat acgaaccgtt gccgggtagg
     3781 gacagaccga gtgcttcggt caggcagttc atcgaatttg ccgtaaacat acccgaacac
     3841 gaaccgcagg tcgggcaggc gttttgttcg acttcctcga cttgccggtt gctgacattg
     3901 tcgtccgccg attcgatcat cgcgtcaatc aagtccaaac ggcgttcggg ttggatgttt
     3961 gccacgccga taactttgcc cgcttccatc gggccaccgg aaacgaagat ggtcgggata
     4021 ttcaggcgca tggcggcaat cagcattccc ggggtgattt tgtcgcagtt ggaaatgcac
     4081 accagcgcgt cggcgcagtg ggcgttgacc atgtattcga tggagtcggc aatcaaatcg
     4141 cggctgggca gggaatacag catgccgctg tgtcccatag cgatgccgtc gtcgatggcg
     4201 atggtgttga attctttggc gatggcgccg gctttttcga tttcgcgggc aaccagctgg
     4261 cccatattgt gcaggtggac atgacctggc acgaattggg tgaacgagtt ggcaacggcg
     4321 ataatcggtt tgccgaagtc ggtttccatc acgccggtgg cgcgccacaa tgcgcgcgcg
     4381 cccgccatat tgcgaccgtg ggtggaggtt ttggagcggt agtccggcat agtgtgtttc
     4441 cttgtctgct atgccgtctg aacggcaaaa ggctttcaga cggcattttt gttgtgtttt
     4501 gaaatgaaaa aaaaggtatt ttataccaag tatcggaatt ttgcgggatt gaaacggcgt
     4561 gcggcaaaaa agaaaatccc cgcaggaatg cggggacggg ttcaggcgcg ggcaatcgcg
     4621 acggctttgg atgcgtccca aaaatcaacg ggggcattta atacgggttt gacgatgccc
     4681 gtccgtatgg cgaaatcgcc gaaaccttcg ccgatattgc gttctgccgc ccatttgccg
     4741 atcaggtcgt ccaattcggc aaggatttcg ggcagggtga tgttttcttt gtaaaggcgg
     4801 gggatgcgta cgccttcacg gtcgccgccg atatggaggt tgtagcgtcc gacggctttg
     4861 ccgaccagcc cgatttccgc caacattgcc cgtccgcagc cgttggggca gccggtaatg
     4921 cgggtaacga tatagtcgtc cgacgtgccg tgtttcgcca taatcttatc cagatcgtcg
     4981 atgaagtctg gcagcacgcg ttcggcttcc gccattgcca aggggcaggt cggaaaggaa
     5041 acgcaggaca tcgcattttc gcgcagcttg ctgacatcgt tgcggattaa tccgtatgtt
     5101 cgggcaagtt cttcgatttt tgctttgtct gcttcggcaa catttgccac gatgaggttt
     5161 tggtttgcgg taatgcggaa atcgcctttg tggattttgg cgatttccaa cacgccggtc
     5221 agaagctgtt tcccgccttc gtcaaccaaa cgcccgcttt cgatgaaaag ggtcaaatgc
     5281 cagttgccgt ctatgccttt cacccagccg atgcggtcgc cgcgcccggt aaatttgaac
     5341 gggcgtatgg gttcgaacgg catacccata cggcgttcga cttccgcgcg gaagttgtcc
     5401 aagcccatat tttgaatggt gtagcgggtg cgggcgtttt tgcggtcgct gcggttgccg
     5461 aagtcgcgct gtgtggttac caccgcttcg gcggccttca gcgcgtgttc cggaggtacg
     5521 aaacccagtt ccagtgaaat gttcggatag gttttggtgt tgccgtgttc cattgaaagc
     5581 ccaccgcctg ccaaaacatt aaaaccggca agctgtccgc cgccgtctga aatggcgaca
     5641 aaatccaagt cgttgccgta gcagtccaca tcatttaagg gcgggatgac gactgcggtt
     5701 ttgaatttgc gcggcagata ggttttgccc aaaatcggct cgtcttcttg aaggaagtcg
     5761 tcggaacttt gaactttttt gccgtccacc caaacatcca gataaccgcg cgtgcgcggc
     5821 agcaagtgtt cggaaatctt tttcgcgtat tcgtaagcct gttggtggag tgccgactcg
     5881 atgggattgg aggtgcaaag cacgttgcgg ttcatatccg ccgccgtggc gatggaatcc
     5941 aaacccagtt tgtgcaagag gcggtgcatc gtctgcaact tggttttcgg cacgccgtga
     6001 aattggaagg tttgccggtt ggtcagcctg atggatcggt aataactgtt ttcgcgggcg
     6061 aatttgtcca attctatcca ttgggacggc ttgatgatcc cacccggcag ccggcagcgc
     6121 aaaagcataa atttcaaggg ttcgagtttt gcctcggcgc gttcggcgcg gatatcgcgg
     6181 tcatcctgct catacatgcc gtggaagcgg atgagttgga aattgtcgcc cttgaagccg
     6241 cccgtgagct ggtctttcaa atcgtccaaa atcgtgccgc gtaaaaaatt gctttcggtt
     6301 ttcaggcgtt cgttgttgga tagcggtttt tcttgccact ccaaaccttt tgtcttggtc
     6361 tgtacggtca ttttgtgttc cttccgattt catttaatca ataaacatca cgctgatagc
     6421 gtttttcttc acgcagcgta tttaaatatt cttctgcgtc gtcccggttt aaatgtcctg
     6481 cttgaataat cacatccaat aaggcggctt cgacatcttt tgccattttt gccgcatcgc
     6541 cgcacacata aacatatgcg ccctcctgca accattgcca aagctctgcc gcctgttcgc
     6601 ggattttgtg ctgcacatag attttttctt cctgatcgcg ggaccaggcg aaatcgtatt
     6661 tatgcaggaa accctctttg gcaaactgct gccattcggt ctgatagagg aagtcggcgg
     6721 caaaatgcgg attgccgaaa atcagccagt ttttgccttc cgcattttct gcggcacgtt
     6781 gttggacgaa agcgcggaac ggtgcgacgc cggtgcccga gccgatcatc acaatcggct
     6841 tgcggctgtc ttcgggcagt ctgaagccgt cgttgcgctc cacaaataca cgcaccgtgc
     6901 cgtcttcttc gacgcaatcg gcaagaaaac cggatgcgcc gcccgttctg gcgcggcctt
     6961 cgtgttcaaa acgaaccacg ccgacagtta aatgcacttc atcgcccact tccgcctgtg
     7021 ctgaagaaat cgaatacaaa cggggtgcaa gcggacgcag taaacggatg aattgttctg
     7081 ccgtcaggct tgccgggaag cggtgcagca catcgacaat aggcgtgttt tgcacgaaat
     7141 cctgcaaaac ggcgttatcg gcaatgattt tatcgagttc ttcataatgg gcgaacgcgg
     7201 catagccttt gacgaaagcc ggagtgtttt gcgtgagttc gaaatgagat gaaagtgcgc
     7261 gcgcaaccgg catcatcttt ccgcctgcct gtatttccgt tgccggatcg atgccgagca
     7321 ggtctaggat ttccccgacc agtgccggat tgttgtcaaa ccaaacgccg agcgcgtcgc
     7381 ctgcctgata gcttaaatcc gaaccgctca aatcaagttc gatgtggcgc acgtctttat
     7441 cggattggcg ggcggtgatt ttctgattgg ccagcagggc ggcgggaaag gggtctgcct
     7501 tgcagtacct gctctccggt accgtctgaa ggtcggcggg gggcattgcc tgtggcgcgg
     7561 gtgttgcccg gttttttgcg gcttcttctt ttaagagtgc ggcgatattg tctgtccagg
     7621 cgtttgcaga ggcggtaaag tccaaatccg catcaacgcg ttcgagcagg cgtttcgccc
     7681 ccaattcttc aaaacgccgg tcgaaatctt tacctgcctg acagaaattc ggataggagc
     7741 tgtcgcccaa gcccagtacg gcaaattgga gtttgtccaa tttcggggct tttttgccgt
     7801 tcagcagttt gtgcagcacg acggcttctt ccggcggttc gccttcgccc tgggtggacg
     7861 taaccagcag caggcggcgt tcgtctgcga tgtttttcgc cttgtagtct ttcagttcgg
     7921 cgagcctgac ttgtatgccg gcggctttca ggctgtccgc cgctttgtcg gcaacggatt
     7981 tcgcattgcc ggtttgcgag gcggaaagga cggtaacgga aaagggttct gccgccggca
     8041 atgctgtctg aagcgcgggc agccctgcgg atgccccgtt tcctgctttt gcccaagcat
     8101 agccggacag ccacgcccat tgtgccgcgt ccagccccga caggagctgc gtgatttcgg
     8161 gcggcagagg cggtaatggc ggatttgtgt tctgcatatc gtgttcactc ataaaatcat
     8221 acctgccgca acagtgccgt atgtcgattc gtctatcagg ataaacgaac cggcggcggt
     8281 gttttccgca taaggcgttg ccgtaacggg tttttgaagg ttgatgcgga ctttggcgat
     8341 gtcgttcatc ttcaaggatt ccgcgccggc ctcttgttcc agcgtgcgga catccaaaac
     8401 gctttcaatt tccccgactt ttgccggcac ggtttgcgtg ccgtgcttga gcaggtattt
     8461 gcgcgcggtg ttgagcggac gttcgtcaaa ccagcaaagc gtggcttcca gatgtttttg
     8521 cggggcgagc ggggaatttt tatcgacaaa aaggtcgccg cgcgaaacat cgatgtcgcg
     8581 gtccagccgg agggttgccg cctcgccggc aaaagcctgc gccacttccc ctttcggcgt
     8641 gatgatttcg gacacttcgg cggtcagccc gttcggttcg atgcggacgg tttgcccgac
     8701 ggcgaccgaa ccgcgttcga tgcgcccctg atagcctcgg aaatcatcgg ccttgtcggc
     8761 atcttggcgg acgaccagtt gcacggggaa ataaaaatcg tcggcggtgc ggctgacttc
     8821 gtccgccccc ggcagggttt ccaaaatgga caataagggt tcgcctttat accaaggcat
     8881 attgccgccg gggtaaacaa tgttgtcgcc caagagtgcg gacaccggta cgaaatgcgc
     8941 gtctttcaaa ccgagctgtt cggcaagtcg gcggtatgcc tccacaatgg cgttgaattt
     9001 gtcttcgctg taatccagca ggtccatttt gttgaccgcc accacaatat gcgggcagtt
     9061 gagttggcgg aggatggcgg aatggcgttt ggtctgcggc agaagctgta agggctgcgc
     9121 gccgaaatcc agttgggatg cgtcaaccag tacgactgcc gccgaagcgg tgcttgcgcc
     9181 cgtaaccata ttgcgcgtgt attgttcgtg ccccggcgtg tcggcgatga tgaatttccg
     9241 tttcgccgtg gaaaaatagc ggtatgccac atcgatcgta atgccctgtt cgcgttcggc
     9301 ttccagtccg tcggtcagga tggagaagtc tatggtttct ttcaaacctt tgcttttgcc
     9361 ggattccaag gttttgattt ggtcggacag cagggctttg ctgtcgtaga gcagccgtcc
     9421 gatcagggtg cttttgccgt catcgacgct gccggcggta atgaagcgga gcggggcctg
     9481 gtgttgtgcc gtcatatttt cttcctcata tctgcttaaa gggtttttga aatttagaaa
     9541 tagccttctt ttttgcgttt ttccattgcc gcctcgcttg cctgatcgtc cagccgggtc
     9601 gcgctgcgtt cggaaatgtc ggcaaccgct gtttctctga taatctccgt cggcgtggac
     9661 gcggtgcttt ctaccgggca ggtgcagctg atgtcgccga cggtgcggaa gcggacatca
     9721 aggatttcgg aggtttcaga cggcattatt ccttatttct tctgtctgat atttatgaat
     9781 tatttgtgca gcccgcattc tttgctgttt ctgccttccc accaccaccg cccggcgcgg
     9841 atgtcttcgc ccgccttgac ggggcgggtg caggggtcgc agcctatgct gggaaatcct
     9901 tgccggtaca aatcgttgta aggcacattg ttggcgagga tgtatgccca cacgtcgtgt
     9961 tccgaccagt cgaaaatcgg gttgtatttg ccgatgtccc gtccggcatc gtattcggca
    10021 aacggcagtt ccgtgcgtgt ggcggactgt tcgcggcgtt gcccggtaag ccaggcgtcc
    10081 gcgcctgcaa tggcgcggtt gagcggttcg atttttcgga tgcggcagca ttcgcggcgc
    10141 gcttcaacgc tgtcgtaaaa ggcaaacctg cctttgcttt ccacataacg gtcggcatct
    10201 tctcgaaccg gccggaaacg ctttatccgc aaatggggat atgcgcgtcc gagtctgtcc
    10261 agcaggttta gggtttccgt gtggagcagc cccgtatcca aggtaaaaat gccgatattg
    10321 aggttttcgc cggcgataag gtcggtaatc accatatctt ctgccgcaag gctgctggca
    10381 aaccgtgcat cccggtggct gccgacaatc cggtgcaggc gttgtttgag ggtttccgtt
    10441 ttttccgcaa gggcggtttc gccgccggat ccgatatgcg gtatctgcca cagggcgggt
    10501 ttgaacagtg tcgtttccat ttttcccgcc ttatgccgcc cgttgtccgg cattcagtcc
    10561 gcccaatgcg ggatacgtct gcccgacccg gttttctcct tcgccgtttt caccgaacca
    10621 ggcgagtttt tcgtgcagcc ccaccacttc gcctatgaca atcaatgccg gattcggcgc
    10681 ggtttcggcg agttcggcaa ggttggcgag cgtgccggtt gcggtttttt gagccggcag
    10741 cgtgccttgg ctgatgacgg ctgctggcgt gtcgggcgag cgtccgtgct gttgcagccg
    10801 ttcggcaatc agggcggctt tgagcgcacc catataaatc accaaggtct ggcggctgcg
    10861 ggcgagggtc tgccattcga tgtcgggcgc atccgccttg cggtggccgg ttacgaaaac
    10921 cgcactttgg gcataatcgc ggtgcgtgag cgggatgccg gcataggcgg tcgcgccgac
    10981 ggcggcggta atgccgggga cgaccgaaaa cgggatctga tggcgtgcca aggtttccaa
    11041 ttcttcgccg ccgcgtccga acacgaaagg gtctccgcct ttcaaacgca ccacgcgcct
    11101 gccttcgcgg gccagcctga ccataagcgc attggtgtcc tcttgcgggg tgcggtcgcc
    11161 ccgggcgcgc ttgccgacaa aaatccgttc cgcatcgcgg cggacgaggg acagtatgcc
    11221 gtctgaaacc agcgcgtcgt aaagcaccac gtctgcctgc tggatttcct gcagcccttt
    11281 gagcgtcagc agccccgcat cgccgggacc cgcgccgacc agcgagacgg agccgccttg
    11341 atcattttga cgactttgtt ccaattggcc tgccaattcc cgttcggcaa gggtgttttg
    11401 ccggtttttg acgagggcgg cgaaacgtcc gttaaactgc ttttcccaaa agcggcggcg
    11461 ttcggtaacg gatttcagtt tgcccttgac ggcatcgcgc caccttcctg aaatttccgc
    11521 catatcgccc aaagacggcg gcagcagggc ttccagcctt tcacgcagca gtcgggcgag
    11581 gacgggcgcg ctgccggagc tggaaacggc aatctgaacc gggttgcggt cgataaccga
    11641 cgggaagatg aagctgcaat ggtcgcggtc gtccaccacg ttgaccggct tttggcagct
    11701 ttcggcaaga tggaaaacgc gccggttgag ggcttggtcg ctgcttgccg caatgatgag
    11761 gaaaaccgtg cggatgtgtt cggcacgaaa ttcttcggca agccacagga ttttgttttc
    11821 cgccgccaac gcggagagtt cggcattcag gtgttttgcg gcaaccctga cctctgcgcc
    11881 cgccttcagc agcaggctga ttttgcgtgc ggcgaccgcg ccgccgccta cgaccaatac
    11941 ggggcggccg gcgaggttgg cgaaaatagg gaaataattc actggctgac tcctttgctg
    12001 tttgcccgca ccttgtttcc gatacggtgc gtcgcggcat ttttgtcgga atgcgggtca
    12061 ttttagacaa aaggattttc ccggttaaat aataaaaagg catttgttag aagctgaaag
    12121 ctatatgagg gcggctgcgg atacggcggt tttcc
//
LOCUS       DAXHTR010000060        11949 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.060, whole genome shotgun sequence.
ACCESSION   DAXHTR010000060 DAXHTR010000000
VERSION     DAXHTR010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.060"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(19..1314)
                     /gene="tyrS"
                     /locus_tag="ACFNML_001110"
     CDS             complement(19..1314)
                     /gene="tyrS"
                     /locus_tag="ACFNML_001110"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFC6392508.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKIALYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     NQLTPFLSFEGGNAAIMANNADWFGSMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLHQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAIEAKDKASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEVLVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            complement(1377..3249)
                     /locus_tag="ACFNML_001111"
                     /pseudo
     CDS             complement(1377..3249)
                     /locus_tag="ACFNML_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
     gene            3423..3626
                     /locus_tag="ACFNML_001112"
     CDS             3423..3626
                     /locus_tag="ACFNML_001112"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392509.1"
                     /translation="MPDGFLQQRGRRFVLQESGRANRKCGRDFILMQPHSGGMETPKT
                     TKTGFRRHFWQSRLKTCRIWVTR"
     gene            complement(3616..4707)
                     /gene="ychF"
                     /locus_tag="ACFNML_001113"
     CDS             complement(3616..4707)
                     /gene="ychF"
                     /locus_tag="ACFNML_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFC6392510.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMAELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVSGKVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDRLKELAAKENAPVVAVCAAMESEIAELEDGEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYDDFVSLGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            complement(4820..6496)
                     /locus_tag="ACFNML_001114"
     CDS             complement(4820..6496)
                     /locus_tag="ACFNML_001114"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HFC6392511.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELNIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKANGAMAALLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLDALEKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RIAVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            6671..7102
                     /locus_tag="ACFNML_001115"
     CDS             6671..7102
                     /locus_tag="ACFNML_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6392512.1"
                     /translation="MGSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGHNWTFLILSGLATGASWLAYFKALQLGNASQVAPVD
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            complement(7333..8028)
                     /gene="murU"
                     /locus_tag="ACFNML_001116"
     CDS             complement(7333..8028)
                     /gene="murU"
                     /locus_tag="ACFNML_001116"
                     /EC_number="2.7.7.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate alpha-1-phosphate
                     uridylyltransferase MurU"
                     /protein_id="HFC6392513.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEEALGDGSAYGVNIAYSPEPEGGLETAGGIARALPLLGDAPF
                     LVVNGDVLTDIDFTAAFQAASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSVRPEVN
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRNEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(8046..8546)
                     /gene="hpaC"
                     /locus_tag="ACFNML_001117"
     CDS             complement(8046..8546)
                     /gene="hpaC"
                     /locus_tag="ACFNML_001117"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042537 - benzene-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HFC6392514.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVAP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTNLSPEERFAY
                     HIWHYGKTGQPEIEGALAHLHGHIVGQHEIGTHFVFYVRLDEIKNCVGKRPALLYFRR
                     QFRSLD"
     gene            complement(8661..9101)
                     /gene="nadR"
                     /locus_tag="ACFNML_001118"
     CDS             complement(8661..9101)
                     /gene="nadR"
                     /locus_tag="ACFNML_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HFC6392515.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            complement(9444..9953)
                     /locus_tag="ACFNML_001119"
     CDS             complement(9444..9953)
                     /locus_tag="ACFNML_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HFC6392516.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWIRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDKAILVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSAS
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            complement(10032..10107)
                     /locus_tag="ACFNML_001120"
     tRNA            complement(10032..10107)
                     /locus_tag="ACFNML_001120"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10072..10074),aa:Lys,seq:ttt)
     gene            10318..11397
                     /gene="apbC"
                     /locus_tag="ACFNML_001121"
     CDS             10318..11397
                     /gene="apbC"
                     /locus_tag="ACFNML_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFC6392517.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGGEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVDDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNVPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
BASE COUNT         2774 a         3280 c         3198 g         2697 t
ORIGIN      
        1 atttctgcgg caatcggatt atttccaaac caaaagcgcg tggttgcgtt tgccgcgccg
       61 aaggatggtg tatttgccga aacgtttgtg ttcgtcggtc agcaggcagg catcgtcggg
      121 gcgttcggcg gcgtggttgg ggttgttggc ttcggcaggt ttgccgttga gcaaaaccgc
      181 tttgctgttc acaaagccgc gcgcttcttt attggaggat gccaaaccgg tttttaccaa
      241 gacttcgacg acattgatgc cgtctgaaac ttcaaatgca ggcagaccgt cgagggcgag
      301 ctgctcgaag tcgctttcgg tcaggctgct ttggtcttcg gcaaacaggc tttcggaaat
      361 gcgttgcgcg gcggcaaggg cttcttcgcc gtgaatcagg cgggtcattt cttcggcgag
      421 gatgcgttgc gcttcgggct tgctgccgct tgccttgtct ttggcttcga tggcatcgat
      481 ttcttcgatg gacaggaagg taaagtattt caggaattta tacacatcgg catcggcgac
      541 tttcagccag aattggtaga actgataggg cgaggttttt ttcgcgttca gccataccgc
      601 gccgccttcg gttttgccga atttggtacc gtctgatttg gttaccaaag gcagggtcag
      661 accgaatact tgtttttggt gcaggcggcg ggtcaggtcg ataccggcgg tgatattgcc
      721 ccattggtcg gagccgccga tttccaaaac cgcgccgtgg cgtttgttca actcggcgaa
      781 gtcgtaacct tgcagcaggg aataggcgaa ctcggtgaag gaaatgcctg cgccgtcgcg
      841 gtcgatgcgc tgtttgacgg attctttgtt cagcatggcg ttgacggaga aatgcttgcc
      901 gatgtcgcgc aggaagtcaa ggcagttcat gctgccgaac cagtcggcat tgttcgccat
      961 aatggcggca tttccgcctt caaagctcaa gaaaggggtt aattggttgc ggatactttc
     1021 cacccagccg gcaacagttt cggcggaatt caagctgcgt tcggcggctt tgaagctggg
     1081 gtcgccgatc ataccggtcg cgccgcccac caaagcaatc ggcgtatgcc ccgcctgttg
     1141 gaagcggcgc aatgccaata cgggcagcag gtgtccgatg tgcaggctgt cggcggtcgg
     1201 gtcgaagccg caataaaggg caattttttg ttcgttcaac aaagcgtcta aggcttcgat
     1261 gtcggtggtt tgcgcgataa ggccgcgcga ttgcaggtct tggatgacgc tcatcggtct
     1321 ctttcaaaaa aaattagcgt ttttgcaaac cgccgattgt aacaaattta agcgaatcaa
     1381 tggttatggc gcgtatcgag aaaccgttgt ttttcggaaa aacgctttgc caattccgtg
     1441 ccgccgtaag ggttgatgtg gtctttgtcc gagtaaaccg gcaatccgcc gatttgaaaa
     1501 tctgcgggga tataggcggc ggcatcaata atatagacgt tggggtattt ggctgccaat
     1561 tccctgatgc gtgcattggc tttcagggtg ctttcgtcgt ccgggcgcag ggcttggcgg
     1621 taacccggta tgcgtgaaga caagatatag gcgcgctgga cgttgtaaga cgaggcaagg
     1681 ttgtccgcca tcaggtaaac ggcttgtttt tcggacgaga gtttatgcag catacggtcg
     1741 aatttttgga aaaaaccggc atcataggca agggagcggc tgttttcggg catttggctg
     1801 ccccagcgca tcgccaaaac cacttttgaa taccggggca ggtgttcttc ggcatagcga
     1861 taaacggcgc ggcaggctgc ccagttttgg aacacacggg acgcgtagcc ttccacatag
     1921 gcgcaagcgt cggcggaaac catagtggcg gaccattttt cttttttgcc cacggcatcg
     1981 aagaatgttt tgtaatggtc ggcgtgggag tcgcccaaaa ccagcagttc cggctgtttt
     2041 tccgtatccc cccataggca ttgtttgccg gtattgttgt ggcaggaggt gttggaacgc
     2101 gtcagcccca agcggtcgta ttgcgccata aacggcagtc tcatcgcaaa aaacgagccc
     2161 gcccccaaaa tgagcatagg caaggcataa atccataaaa cggattgtgc gaacgaacct
     2221 tgccattttt taaacggttt ttcgatgcag tggtaagaaa acagggaaag cagcaatatc
     2281 aggacgaccg ccgccgccgg cgaataaggc ggcaggttgt ccgggccgat atagcgcata
     2341 aaggccaata tcggccaatg ccacagataa agcgaatagg aaatcaaacc ggcggcaaca
     2401 gtgattttcg attggaaaaa ttttttaagc gggtgttcgt aatgattgaa ataaatcagc
     2461 gcggcaacag ccagacaggg aatcaaagcg gcggggcccg ggaaataggc ggtttgttcc
     2521 gaataggaaa acaggcaggt tgacaatatg cacacggcaa acaatgcgcc gacggcggca
     2581 cagcgtctgc cgacggcagg ttgccggcag cgcatccaca cggcggtcag cgatcctatc
     2641 agtaattcgc aggcgcgcag gtggggcagg taatatttat cgagcgcgga aggtataaag
     2701 gaggcggcaa ggcttaaggc acacagtgcg gcaaggaagc cgaactgtac gcgcaggctt
     2761 ttgcgggcga caagcagcag cagtatcgga aagacaaagt aaaattgttc ttcgaccgac
     2821 aaagaccaga tgtgcagcag gggcttttct tcctgcgcgg gatcgaaata atccttcccc
     2881 cttgcaaaat acaggttaga ggcgaaaccc aaggcggtca gcgcggattt ccacaaaaga
     2941 aagaaatcat ctttggtgaa taaaaagaag ccgcctgcca gcgttgccgc caatacggcg
     3001 aaaaatgcgg gcagaatccg cttgatgcgg cggatataaa atgccttcag ggaaaacctc
     3061 cccccccccc cccgacattt cgcggtgaag aatcgtcgtc atcaaaaagc ctgaaatcac
     3121 aaagaatata tcgacaccga gaaacccgcc cggcagccaa tccttttcga tatggaacac
     3181 gatgacggac aagacggcgg cggcgcgcaa tgtgtcgatg tccgggcggt agggtaaggc
     3241 ttggctcata atgtttttat agtggattaa atttaaacca gtacggcgtt gcctcgcctt
     3301 gccgtactat ctgtactgtc tgcggtttcg tcgccttgtc ctgatttaaa gttaatccac
     3361 tatactcgaa acgcggcggc gcaaatgccg tctgaaaggt cattttgtac gggatattcg
     3421 gaatgccgga cggatttttg caacagcggg gcaggcggtt tgttttgcag gaatcgggga
     3481 gggcaaatcg gaaatgcgga cgggatttta ttttgatgca gccgcattcc ggcggtatgg
     3541 aaacgccgaa aaccaccaaa accggcttca gacggcattt ctggcaaagc cgtctgaaaa
     3601 cctgccgcat ttgggttaca cgttaaacaa aaagtgcatc acatccccat cctgcacgac
     3661 gtattcctta ccttccacac gcattttgcc ggcttctttg gctttggctt cgccgccgag
     3721 cgagacaaag tcgtcgtaag aaatgacttg ggcgcggatg aagccgcgtt cgaagtcggt
     3781 gtgaatcacg ccggcggctt gtggggctgt gtcgcctttg tgtatcgtcc acgcgcggac
     3841 ttctttgacg ccggcagtga agtaggtttg cagccccaaa aggtcgtaac cggcgcggat
     3901 caggcggttc aggcccggtt cttccaagcc catttcggcg aggaactcgg ctttttcacc
     3961 gtcttccaac tcggcgattt cgctctccat cgcggcgcac acggcgacga cgggggcgtt
     4021 ttcttttgcc gccaattctt tcaggcggtc gaggtgcggg ttgttttcaa aaccgtcttc
     4081 ggaaacgttg ccgacgtaca tggcaggttt ggcggtcagc aggaacagcg gtttgagcat
     4141 cgcgcgttct tccgcgtcca aaccgaagga acgcacgggt ttgccttcgt ccagatgcgg
     4201 cagcagtttt ttgcacaaat cgaccagctt ttgcgcgtct ttgtcaccgg aacgggcgcg
     4261 tttttcttcg cggacgatgg ctttttccac acttgccaag tcggcaagcg ccaactctgt
     4321 accgatggtt tcgatgtcgg caatcggatc gactttgccg gaaacgtgga cgatgttgtc
     4381 gtcgtcaaag cagcgcacga cattcacaat cgcatcggtt tcgcggatgt tggcaaggaa
     4441 ctggttgccc aagccctcgc ctttgctcgc gcctgcaacc aaaccggcaa tatcgacaaa
     4501 ttcgacgatg gcaggctgca ttttttgcgg attgacgatt tttgccaatt cggccatacg
     4561 cggatcgggg acttcgacga tgccgacgtt gggttcgatg gtacagaaag gatagtttgc
     4621 cgcttcgata cccgattggg tcagcgcgtt aaaaagggtg gatttgccga cgttgggcaa
     4681 accgacgatg ccgcatttca aactcatgtt ttttcctgaa aatagagaaa tttaacggcg
     4741 gattatagca taccgccgcc cgcgttccga aaaatgccgt ctgaaacggc ttcagacagc
     4801 atccggtttc aaaaaccgtt cagaacaagc cgtgaatcac gccgtgttcg tccacatcga
     4861 ttttctcggc ggccggaact ttcggcagac ccggcatctt catcatattg ccgcacagcg
     4921 cgacgatgaa gcccgcgccg gcggaaacgg tgatgccgcg cacggcgatg cggaagcctt
     4981 cggggcagcc caacagtttg gcgttgtcac tcaaagagta ttgggttttc gccatgcaga
     5041 tcggcatttt gtccaagccc agtttttcca gtgaagcgat ttcggcagac gcttccgcgc
     5101 tgaaatcaac atcttccgcg ccgtacactt tttgggcaat cgcacggatt ttgtctttga
     5161 tgcccaactc gacatcgtag gcgaaaccga agttattggt ttgactttca atggcgttga
     5221 cgactttgcg cgccaaatcc gcgccgcccg caccaccttt gccccacact tcggtcaggg
     5281 aaacttcaac gccgtgttcg gcacaggctt tttcaatcat cgccaactcg gcatcggagt
     5341 cggacacgaa gcggttgagc gcaacgacga cgggcagtcc gaatacgttt ttcaggttgg
     5401 aaatgtgttt cagcaggttg ggcaaacctt tttccaaagc gtctaaattt tcttcgccga
     5461 ggttggcgcg ttccacgccg ccgttatatt tcaacgcgcg gacagtcgcc acgacaacag
     5521 ccgcatcagg tttcaaaccg gcaaggcggc atttgatgtc gcagaatttt tccgcgccca
     5581 agtccgcgcc gaagcctgct tcggttacgg cgtaatcggc aaggtgtttc gccagacggg
     5641 ttgcggttac ggagttgcag ccgtgggcga tgttggcgaa cgggccgccg tgtacgaagg
     5701 cgggcgtgcc ttcgatggtt tgcaccaagt tgggcttaat cgcatcttta agcaatgccg
     5761 ccatcgcgcc attcgctttc aaatctttgg cgtaaacggg gctgccgtct ttggcgtagg
     5821 cgacaaggat gttgcccaaa cgctctttca aatcgctgat gtctttggca agacagaata
     5881 ccgccatcac ttcggaagca acggtaatat cgaaaccgtc aggacgcatc acgccgtcaa
     5941 caggcttgcc catgccgtcg atgatgttgc gcaactggcg gtcgttcata tcgaccacgc
     6001 gccgccacag cacgcgtttg gggtcgatgt tcaactcgtt gccttggtag atatggttgt
     6061 cgagcatcgc ggcaagcaga ttatttgccg caccgatggc gtgaaaatct ccggtgaagt
     6121 gcaggttgat gtcttccatc ggcaaaactt gggcatagcc gccgcctgcc gcgccgcctt
     6181 tcacgccgaa caccggccct aaagaaggtt cgcgcagggc aatcacggca tctttgccga
     6241 tgtggcgcaa cgcgtccgcc aaaccgatgg ttacggtggt tttgccttcg ccggcaggag
     6301 tcgggttgat ggcggttacc aaaatcagcc tgccttgttt ttgcggcagt ttaaacgctt
     6361 cggcaggatt gattttggct ttgtaatgac cgtaaggctc aatgttgtcg gcgttcaggc
     6421 ccagcttggc ggcaatttcg ccaatcgggc gcatggtgga agactgggcg atttcggcat
     6481 cggttttgaa gctcatgatt ttcctttaga aatgaggagg gacataccgt ctgaaagcat
     6541 caggcgacaa acaggtggat tgaaaataat atcaggcata ttataacgtt atccgcacca
     6601 aacccgcagt gaaatttttg acgctgcaac aaaaataccg ttcatattgt tcacaatcca
     6661 aggagaaaac atgggcagca acgcatggct gttttgggca ttggcatcgg caggcttcgc
     6721 ctcattgacc gccgttttcg ccaaaatggg cttgcagggt atagattcag atttcgctac
     6781 cttcatccgc accttggtca tcctcgccgc tttggtactg tttttgacct acaccggcaa
     6841 atggcagggc gtgaacggct ttacggggca caactggaca ttccttatcc tatccggcct
     6901 tgctaccggc gcatcttggc ttgcctactt caaagccctg caactgggta acgcctcgca
     6961 agtcgccccc gtcgataaat tcagcctggt tctggtcgcg ctgatggcgg tggttttctt
     7021 ggacgaacgc ccgaacacgc aggaatggat aggcttgggg ctggtaacgg cgggcgtgtt
     7081 ggtgctggcg ttgaaacgtt aaaccgaatc cgccataccg cctgaaaccg ggttttgaca
     7141 atctgacggg cggcggtctg aacaaagtta cccaataaaa tccgacaccg cgccgacggc
     7201 aaaccgccgc gcttcagatg gcatttgttg gacggaaagc cccgaacgcc ctattttcac
     7261 gcctccacga aaatatgccg gacatccggt cggaaccgtt cggcagccgg ctcggcaagc
     7321 caccgtttta tttcacttcc aagcccctgc aagggcttga gcctctttca gacggcatac
     7381 cgtgccgaca tccagccaca agcccgtatg cttctgaccg ctcacgcggt tttgccgcat
     7441 ttcgttccgc aatacgggcg cgagtttcgc cacactgccc gcttcgattc cgtcaaacat
     7501 ttcaggacgg taaataccca cgccgctaaa tgtcaatccg ttgccgccat ttacttccgg
     7561 ccgcacgctg ccgtcgggca gcagggaaaa atcgccgtca ggatggtgcg ggggattttc
     7621 caccagccac agatgggcgg aaatgtgctc gggcagggac gatgccgcct gaaacgcggc
     7681 ggtaaaatcg atgtcggtca acacgtcgcc gttgaccacc aaaaacgggg catcgcccaa
     7741 cagcggcaat gcccgtgcga taccgcctgc ggtttccaag ccgccttcag gttcgggcga
     7801 ataggcgatg ttcacgccat aagccgagcc gtcgcccaaa gcctcttcta tctgccgacc
     7861 cagccaagcg tggttgatga cgatttcggt aaaccctgcc tgcttcagac ggcataggtg
     7921 ccaaccgatt agaggcttac ccgccacatc gagcagcggc ttcggagtgg tatcggtcaa
     7981 agggcgcata cgctcgccgc gtcctgccgc cagtatcatc gctttcatat atatctgtcc
     8041 gaatatcagt ctaaagatct aaactgccgt ctgaaataaa gcagcgcggg acgtttgccc
     8101 acgcaatttt taatttcgtc gagcctgacg taaaacacaa aatgtgtgcc gatttcatgt
     8161 tggccgacaa tatgcccgtg cagatgcgcc aacgcgcctt ctatttcagg ttgtccggtt
     8221 ttaccgtaat gccaaatatg ataggcaaaa cgttcttcgg gtgaaagatt ggtcaatccg
     8281 gcaaaatgtt cggccacatc ctgatgttcg tctgccaacg tattgatgca caggctgccg
     8341 ttttccgaca ggatcggaat gattcgcgca ctccggttga tgcacagcat cacggtcggc
     8401 ggctcgtcgg taaccggcgc gaccgccgtc attgtaatgc cgtaacgccc tgccgcaccg
     8461 tctgtcgtga tgacatgaac gcctgccgcg caagatgcca tcgcatcacg gaacgaagtt
     8521 tgaaaagttt tctgcaaatc cgccattttt cccctttaaa ctgtccccta tataagaatg
     8581 ctgcacacaa ggcacccccc atgtgcagca gttctgattc aaaaagccgt cggtcggaca
     8641 tttccgcgcg ttacggcgta ttacgagttc aacgcatcct cgattttggc aagttctgcc
     8701 aacaggtctt taagcagcag cattttctcg cggcccagca cttcctcgat agcgtcgtaa
     8761 cgctcgtcca cttcttcgcc gatttcctca tacagcttct cgccctcggc agtcagcttc
     8821 agaaaaacac gtcgttggtc gttggaaggt ttcaggcgga caaccaaacc cgctttttca
     8881 aggcgggtca ggataccggt caggctgggg cgcaaaatgc acgcctgatt cgccaaatct
     8941 tgaaagtcca gcgtgccgtt ttccgccaaa agacggataa tccgccattg ctgatcggta
     9001 atattcgcct gattcagaat aggcctgaat tgggtcatca gggcttccct tgcctgtatc
     9061 agactgatat tgatagacgc atgttttgat tgggtaggca ttgttcaagt ctccaagtta
     9121 tcgaaaatca aactttcaaa ccgtcgggaa agcctgtggg cgtaattttt gatgcaaccg
     9181 ttatataaca aaacgaacat atagcaacaa tacgctataa accgcatcgg acgactgggt
     9241 ataaaagact ttaattccga taatcctatc taaaaatatt ttaatagtta tatcttagtc
     9301 tattttccct acaatctcaa caaagtatta catttactgt cgaaagaact ttcttccaga
     9361 aaaaacaaaa gccgccgcat cagacgagca aggtatatgc cgtttgattc tctacatagc
     9421 gtaaagttta acaaaaacaa aagttaaccg aaaacatccg cctgaaaaat tcgtgcgcgc
     9481 aagccccaat aactgctgat tcccgtcgta tagtgaacca ttttcccatt tttgaccaaa
     9541 acgacggcag gcgttgcgac aatccgccaa gaccttgcca aacccccgtc ctcatcgttg
     9601 accgtcggaa agcccaagcc ccgctttgcc atatacgccg ccacttcgga cgcgctgccg
     9661 gaacgtaccg ccacgccgac gaccggaacg ccgtccgccg ccaaatcatc gattatcggc
     9721 gactgataac ggcacacgcc gcaccagctt ccccaaaaat acaccaaaat cgccttatcc
     9781 cggctaaact gtcccaaagt cagccgctgc cccgacagca gggtcaaagg ccgccctgcc
     9841 gcaccggccg gctcttcggg cttgcgtatc caatccaaaa acagcgacac caataaaaac
     9901 accaatgccg tctgaacggc aaatttgatg cccgaaagca gtttcttttt catacgctct
     9961 ctcaaacggt acgcccgcgc aaccggcagg caaacaaaaa gccaagtctc aaaacttggc
    10021 ttccggttat ctggtgggtc gtgagcgatt cgaacgctcg accaacggat taaaagtccg
    10081 ctgctctacc ggctgagcta acgacccgat aagccgtgca ttatacagca ccatcctacc
    10141 tcgtcaagca aattttacag gcttaattgc agaccactgt ttgcacggga tattttgaca
    10201 acggattttc acaatccgcc gcataccgtg taaaagttcg cacaaggaaa agcaaaccgc
    10261 ccgaaatcaa tgtacacttt ccgcccgttt cccaaactgc acacagaaac acacattatg
    10321 aacatacaaa acatccgcac cctcctcgac accgtcgccg ttccgaatac ggcacgcacg
    10381 ctcggcggcg aaaaggccgt ccgttcggtc gaacagcgtt cagacggcat ccatatcgcc
    10441 ctgcatttcg gcttccccgt cgcgcacatt gcctcagaaa cagccgaccg catacaggaa
    10501 atcctgatgc cgctgacagg cgacacacac atccatctgt ccatggacac tgaaatcggc
    10561 acacacaaag tccagcccgg cgttaccact attaaaggcg tgaaaaacat catcgccgtc
    10621 gcctctggca aaggcggcgt gggcaaatca accaccaccg ccaaccttgc cgccgcaatg
    10681 gcgcgcatgg gcgcgcgcgt cggcgtactc gatgccgacc tttacggccc gagccagccc
    10741 acgatgctgg gtgtggacga ccgcaaaccc gatcagaaaa accaaaaact cattcccgtc
    10801 gaatcttcag acggcataca ggtcatgtct atcggctttc tcgtcgatac cgaccaagcc
    10861 gtcgtctggc gcgggccgat ggtcagccaa gccttgcagc aactgatgtt tcaaagcgag
    10921 tgggacgaag tggattatct tttcatcgac ctgccccccg gtaccggcga catccagctc
    10981 acgctgtccc aacgcatccc cgttaccggg tccgtcatcg taaccacgcc gcaggacatc
    11041 gcgctgatag acgcgcgcaa ggctgtggat atgttccgca aggtcaacat tcccatttta
    11101 ggcgtattgg aaaatatgtc cgtccacatc tgcaccaact gcggacacag cgaagccctg
    11161 ttcggcacgg acggcggcaa agatttagcc gcacgcctca acgtccccct gctcggacag
    11221 cttcccctaa gcctgcccgt gcgcgaagca atggacggcg gcacaccggc gcaactgttc
    11281 gacgaacacc ccgccatcgc ccgaatctac accgatgccg cattccaaat cgccctgggc
    11341 attgccgaca aaggcaaaga cttcagcagc cggttcccta aaattgtcgt cgaataaagc
    11401 cgcgtccgaa accgcaacag caatgccgtc ccaagccccg cgcctgccgg cgggcttcat
    11461 agtcctctct ccccgtggga gagggctgga gagagggcaa caagccgcaa ggcttgtatt
    11521 ggggcggtta agctgttggg aaagattgcc gcaacttgga gaatgccctc tccccggccc
    11581 tcccccacag gggagggagc ggattgcggc aggttttgcg gttgcaggcg gtttgaaaaa
    11641 cagattggat ttacaaccgt tgatgcaggt cgtctgaaaa gcaaaaagca gcctgcacaa
    11701 cctgattagt ttacaaaacc cttaacccca acagcctccc cgtcctctct ccccgtggga
    11761 gagggctgga gagagggcaa caagccgaaa ggcttgtatt ggggcggttg agttgttggg
    11821 aaaggttgcc gcaacttgga aaatgccgcc tccccggccc tcccccacgg gggagggagt
    11881 gggtttcggc agatttttcg gttgcaggcg atttgaaagg caacttagat ttgcagctgt
    11941 tgtttcagg
//
LOCUS       DAXHTR010000061        11871 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.061, whole genome shotgun sequence.
ACCESSION   DAXHTR010000061 DAXHTR010000000
VERSION     DAXHTR010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11871
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.061"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..152)
                     /locus_tag="ACFNML_001122"
     CDS             complement(<1..152)
                     /locus_tag="ACFNML_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6392518.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRG"
     gene            complement(303..695)
                     /gene="rpsI"
                     /locus_tag="ACFNML_001123"
     CDS             complement(303..695)
                     /gene="rpsI"
                     /locus_tag="ACFNML_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFC6392519.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(705..1136)
                     /gene="rplM"
                     /locus_tag="ACFNML_001124"
     CDS             complement(705..1136)
                     /gene="rplM"
                     /locus_tag="ACFNML_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFC6392520.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(1314..1493)
                     /gene="ssrS"
                     /locus_tag="ACFNML_001125"
     ncRNA           complement(1314..1493)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACFNML_001125"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(1499..1804)
                     /locus_tag="ACFNML_001126"
     CDS             complement(1499..1804)
                     /locus_tag="ACFNML_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFC6392521.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMATLNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(1815..2144)
                     /locus_tag="ACFNML_001127"
     CDS             complement(1815..2144)
                     /locus_tag="ACFNML_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392522.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(2199..3188)
                     /locus_tag="ACFNML_001128"
     CDS             complement(2199..3188)
                     /locus_tag="ACFNML_001128"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFC6392523.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEERE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWIE
                     ELVPQLNTTVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(3311..6013)
                     /gene="ppc"
                     /locus_tag="ACFNML_001129"
     CDS             complement(3311..6013)
                     /gene="ppc"
                     /locus_tag="ACFNML_001129"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFC6392524.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQMVYPDVCVPNILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNSLNEEQKQAALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKESGLLA
                     VENGKPRSRINIVPLFETIEALENACPVMETMFRLDWYDALLESCGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKVIFGMIKEEYQRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            6056..6949
                     /locus_tag="ACFNML_001130"
     CDS             6056..6949
                     /locus_tag="ACFNML_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6392525.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDAFLLRYSRHILLDEIGIEGQQKLSAAHILVVGCGGLGAAALPYLAASGIGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALADRLKRINYNVDVRTVNEKLDGCRL
                     TGLVQTADIVLDCCDNYATRQAVNRVCVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPICGAER"
     gene            complement(7349..7573)
                     /locus_tag="ACFNML_001131"
     CDS             complement(7349..7573)
                     /locus_tag="ACFNML_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HFC6392526.1"
                     /translation="MDAVQELEHRITELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDTQEPYSLRDEIPPHY"
     gene            complement(7574..8962)
                     /locus_tag="ACFNML_001132"
     CDS             complement(7574..8962)
                     /locus_tag="ACFNML_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HFC6392527.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTVGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(9048..9869)
                     /gene="prmC"
                     /locus_tag="ACFNML_001133"
     CDS             complement(9048..9869)
                     /gene="prmC"
                     /locus_tag="ACFNML_001133"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6392528.1"
                     /translation="MTFDKWLGLSKLPKNEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQRRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARKNAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLSQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(10384..11868)
                     /gene="tldD"
                     /locus_tag="ACFNML_001134"
     CDS             complement(10384..11868)
                     /gene="tldD"
                     /locus_tag="ACFNML_001134"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFC6392529.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVRERLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILVGYMQDETNARLTGTQSTGNGRRESYASVPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
BASE COUNT         2462 a         3254 c         3345 g         2810 t
ORIGIN      
        1 ccgcggtggt ctcggaggta acgggttctt tgacggttca ggtattgttc gatcggttgc
       61 caatcaatca cttgatctag cttcaatagt gggaagcggt cgatgtgttt ggcaatcatg
      121 gcttgtgcgg tttgctgaaa gaaggtgctc atggaaaatc ccctaaatgg cttggtggga
      181 atttagggga ttttggggaa ttttgcaaag gtctcaatct gtaatcttta gattgccaat
      241 gggaaaccgt ctactacaaa taaaaaaccc tgcgataagc agggtttttt gaatttccaa
      301 cattaacgtt tggagaattg ttttgcacgg cgtgctttgc gcagacccgg ttttttacgt
      361 tcaacttcgc gggcatcgcg ggtaacaaaa ccagcttgag acaaggcggg tttcaacgcg
      421 gcatcgaagt cgatcagggc acgggtaatg ccgtggcgga ttgcgccgga ctggccggtt
      481 tcgccgccgc caacaacatt gactttgatg tcgaaagatt cggcgttttc agtcagaacc
      541 aagggttggc gaacaaccat tcggctggtt tcgcgtgcga agaattcgtc aacgggacgg
      601 ccgtttacga tgatttgacc tgtaccttta atcaggaata cacgagccac tgaacttttg
      661 cggcggcctg tgccgtagta gtatttaccg ttcatgtcgc gtccttattt cagttccaaa
      721 actttgggtt gttgcgcagc atgggcgtgt tccgcacccg catacacttt cagtttttta
      781 atcatggcgt aacccaaagg acctttgggc agcatacctt ttacagcttg ttccaaagcg
      841 cggcccggga attgctcttg catttcgcgg aaagtgcgtt cgtagatacc gcctgggaaa
      901 ccggaatggc ggaagtattt tttatcttcg aatttggcac cggttacacg cagtttgtcc
      961 gcattgataa caatgatgta atcgccggta tcgacgtggg gggtgtattc aggtttgtgt
     1021 ttgccacgca gacggctggc gacttcggcc gcaacgcgac ccaagacttt gtcttgggca
     1081 tcgatgacga accattcgcg cttcacctcg tggggtttcg ctgaaaaggt tttcatagtg
     1141 gaaatccaga tagatataga aagttgtaaa ttttaaagac aggattcgat tttgtcaatc
     1201 gcattaccgc gttacggaag gattttccgg atttcggcag actgcatact gctttttcgg
     1261 gcggggcggc ggccaatgtg aaaaaccgca tcgttgcgat gcggttttga atgggaatcc
     1321 ccgcgagagc cgtttcggcc gaatccgctt gaaccttgct gacaaggcgg ctgcctcggg
     1381 tagtttcggg tgcgtccgca aaaggacgct cgcgcccact actgctcccg gcaaccttaa
     1441 gcgaacttat tggttcaaag gaatatatgc cttcgcggac accgcaggga aaaaggggtt
     1501 attcctgcgc caagcgggat agtgcttttt ggcaggcgtt gtccatatcg gctattttac
     1561 gcgcaaaatc gccgattgcc aaatcgccgc cgttcaggga ggttttcaac aggtcgtgga
     1621 cgacgttgag cgtggccata atgacgattt tttcgctgtc cgcgacgcgt ccgccttcgc
     1681 ggatggcttc ggctttgccg ttgagcattc cgactgcctg caacagtgtg tctttttctt
     1741 ctgccggcgt gttgacggtc agccgggcgt gcatgacttc gatgtggact tgttcgatgt
     1801 tcatccttta atccttattg ctgcgtttcc tgccattggg ggaggcgcgc tgccagtgcg
     1861 ctgatttttt ccctgctctg ttcgagcagg ctgcggtatc gtgtattttc ttctgtcagg
     1921 ctgtcgattt tgttttgcag gtcttctttg agtttgccga cttggacgag cagggcttcg
     1981 ctgagttcgt cgacggcggt ttcgtgttcg agtttttgcc gctcgtgcgc ccgtttgagt
     2041 tcggcgacgg tttctttgag gcggcggttt tcgctgacga gggtttcgaa tttttgtacc
     2101 agcgtataaa cgctgctttc gagtttttcg atattttgtt tcataacctt acctgtccgt
     2161 atgccgtctg aaggcctcag acggcatctg tctgttgttt attcgaaacg cgcgctgcgt
     2221 tccatcagtc tttcgacgac ctgctgaggg gtcatttctt tgcggatgag ttgcagcagg
     2281 gtttgggtaa tcggcatgtc gatttggtac ttacaggcag tattgaagac ttcttctatc
     2341 gtgctgaccc cttcggaaac gtgtccgatt tcgaccagca cctgatgcag ttccttgcct
     2401 tctgccaaac ccaagccgac gcggcggttg cgtgagagtg cgccggtgca ggtcaagatg
     2461 aggtcgccga tgccagcaag ccccatcatg gttttgggct gtgcgcccat tgcggaggca
     2521 aggcgggtga tttcggccag tccgcgtgta accagtgcgg cacgggcgtt aagcccgtac
     2581 tccagaccgt cggacaatcc ggtggcaatc gccatgacgt ttttgactgc accgccgact
     2641 gccacgccga taacgtcggt actgccgtaa agcctcatga cggtcgtgtt gagctgcggt
     2701 acgagttctt caatccactc ttggttttcg gaggcgagga cgacggcgca gggcagttgt
     2761 ttggcgagtt cctgtgcaaa actcggaccg gaaagtacgc cgattttttt attgtcgggc
     2821 aatacttctt tcaatacttg aaaggtcagc agccctgtat cctgctcgaa tcctttgcag
     2881 gcggcgagga cggggaggtg tcccgcgccg tactgtttga gcaactctgc gctgcttctc
     2941 aatccggcaa cggaggttac gataaggaca agtccgctgt ctttgagcgc gtctgccaaa
     3001 tccgcacaca cttccaaggt ttcgggaaag gaaaagccgg gcagtccgcg tttgttttca
     3061 cgttcttcct gcatttggcg gacttggtct gcgttgcgcg tccacaggga tacgcggttg
     3121 ccgtgttggg aaaaatgcag ggcgagcgcc gtaccccacg aacctgcgcc gataacggta
     3181 attttcattg gtcgtctttc aacatatcac tgccgttcac tttaaaacaa tcggtgtttc
     3241 tctgcaagtg cggtcaggga aatgccgtct gaaaggcgtt cagacggcat tttgccccga
     3301 tgcggcacta tcagcctgta ttgcgcaaac cttgcgccac gccgttgatg gtcaggtgca
     3361 ccatcagaag ggcgtgcgga ttgtcgggtt ctttacgcag gcgtttgagc atggcgactt
     3421 gcaggccgtt gagggcgttc aaatatggaa tcctcaaggc aagcgaacgg gcgaggctgc
     3481 ggttgtcgcg caggagctct tcggtttgca gcaagtcgag cagtgctttg cggctgcgtt
     3541 ggtattcttc cttgatcatg ccgaagatga cttttgcttt gtcgggtgat tcgctcaagc
     3601 cggcatagtt ttccgccaag gtgatgtcgg ttttcgccat cacttgttcc atattggaga
     3661 gcatggcttg gaagaacggg ttgctttggg cgtgttcgcg cagggcggcg agcgtttcgg
     3721 gtttgtcttc gcacaaggtt tccaccgcgc tgccgaaacc gtaccaagca ggcagcatga
     3781 ggcggttttg catccaggaa aatacccacg gaatcgcgcg caagtcttga atccgcgcta
     3841 aggtcttgcg gctggcggga cggctgccga ggttgagggt ggcgatttcc tgaatcgggc
     3901 tggtttgcag gaagtagtcg atgaagtcgg ggtgggtaat cagttcgcgg tagtatttga
     3961 acgatacgtc cgacaatgcc tgcatcagtt tggcatcagg gtctttttta tccggcagga
     4021 tgctggcttc caaagtcgcg gcaaccaagg tttccaagtt gcgttgggca ttgccggggt
     4081 cggcgtattt ggcggtaatg acttcgcctt gttcggtaat gcggatttgt cccgccacgc
     4141 tgcccgccgg ttgggcgaga atggcttggt aagaagggcc gccgccgcgt ccgacgctgc
     4201 cgccgcgacc gtggaacagg cgcatgcgga catcgtattt tttgaagagt tcgaccaagc
     4261 ccaattccgc ctgatacaga caccatgagc tggtaacata gccgccgtct ttgttggagt
     4321 cggagtagcc gagcataatt tcttggatgt tgccgcagct ttcgagcagg gcatcgtacc
     4381 aatcgaggcg gaacatggtt tccatgaccg gacaggcgtt ttcgagtgct tcgatggttt
     4441 caaacagcgg cacgatattg atgcggctgc gcggtttgcc gttttccacc gccagcaggc
     4501 cgctttcttt cagcagcaat gccaaggcga gcaggtcgct gggttgttcg cagttggaaa
     4561 taatgctttg tgtaacggca tcttcgccga attcgtcttt gattttgcgc gcttcgttga
     4621 aaattgccag ttcgtggcgg gtatggtcgc tgtatgtgat gaacgggctg tacagcggac
     4681 gttgatggct caattcgcgc aacagggcgg cttgtttttg ctcttcgttc aggctgttgt
     4741 agtcttccaa gcctgcgtgt tggaaaagct cggcaaccac atcggcgtgt ttgcctgcgt
     4801 gttggcgcag gtcgagcggc atcatgtgga agccgaacac ggacacggaa cggatgaggt
     4861 ctgccaaacg gccttcagca agcaggcggc tgccgttgtc gataagggaa cgttgcaatt
     4921 ttttcaaatc atcgagaaat tcttgtgccg aagcatacgg ctcgagaaag ccgaatttgc
     4981 agcccatgcc caaaccgagc gcgcgcgctt tgcccatagc gcgtgccatg atgtaggcga
     5041 tggcgcggcg gtagggttct tcggtacggg cgatttcttc gtcaggcgag agggcggcca
     5101 gtgccattac gtcgtcgttg actttgacgc ggcggatgga gagcggcagt tcgcggtaaa
     5161 gtttgtcgag ttcgccacgg taaaaacgga acacggcatc ggcgtggcgg cggaaggcaa
     5221 aacgcaaggt ttcggcagaa acgaacggat tgccgtcgcg gtcgccgcca atccagccgc
     5281 cgattttgag gatattcgga acgcagacat cgggatagac catctgaaag tcgtgttcca
     5341 tcttgcggta gagcttgggc agggcttcga aaaagctcat cgggaagatg gacacgccgt
     5401 tgttgatttc gtcgttgacg ctgagtttgt ggcggcgcgt ttcgctggtc tgccacaagc
     5461 ccagcaggat agtgtcgatt tcgcggcgca gccgtgccag cgcgtcggca ttggtgcagc
     5521 gttcgcgttg cggcaacagt gcgcggatgc ggcggttgaa gcttaagacg gtttggcgtt
     5581 gcacttcggt cgggtgcgcg gtcaaaacgg cggtaacgga cgtattgtcc aactgccgct
     5641 gcaccgattt gccgtcggct ttccccgctt tgagcctgcg gacggtttcc gtcaggctgc
     5701 cttccgcgcc gccgcgtccg gcttcttcgt ggatttggcg gcggcgttcg tggtgcacgt
     5761 cttcggcgat gttcaaaatc tgtgcgaaca ggccgcaggc caaggttaaa tcgtgggttt
     5821 gttgttcgtc caattgcggc aatacttttt caatcaatgc cgcgctgtcg tcggaagtgg
     5881 acaagagttt gaccgtttcg acaaccaatg gcgaggcttc ttcgtgcagg aggttgaaca
     5941 gggattgttt cagaaattcc gcgtccgccg ccaaagccgc gtcctttgga ttgttcagaa
     6001 tatgcagttg catgattttt ctctcattgc cgtaaatact gtaaatgtac ctcaaatgcc
     6061 gcatccgtgc caaaccgttc acactttaac cgcccgtgtc ccgaaatgcc gtctgaagtt
     6121 gaacgccgcc cgacggcagc gttacaatcg cccacaactg ttttttccga acatcatcat
     6181 gaccacgacc gaacacgaca acgacgatgc attcctgctg cggtacagcc gccacatcct
     6241 cttggacgaa attggcatcg aagggcagca gaaactttcc gccgcgcata ttttggtcgt
     6301 cggctgcggc ggtttgggtg ccgccgccct gccctatctc gccgcttccg gcatcggcac
     6361 gctgaccata gccgattccg acacggtcga gctgcacaac ctgcaacgcc aagtcgcatt
     6421 tgacgagggc gatgtcggca agcccaaagc cgaagccttg gcagaccgcc tgaaacgcat
     6481 caactataac gtcgatgtcc gcaccgtcaa cgaaaaactc gacggctgcc gcctgaccgg
     6541 tttggttcaa accgccgaca tcgttttaga ctgctgcgac aactacgcca cgcgccaagc
     6601 cgtcaaccgt gtctgcgtac aaacgaaaac accgctggtt tcaggagcag cggtgcgctt
     6661 tgaagggcaa cttgccgtgt accgtcccga cttgcccgac tcgccgtgtt acgcctgcct
     6721 gtttgacggc ggatcggctt cagacggcat ctgttccctc ttcggcgtgt tctcgccgct
     6781 ggtcggcatc atcggcagta cccaagcggc ggaggctctg aaaatcctgc tggatgcggg
     6841 cgaaccgtcg cacggcaggc tggcggttta ccgtgccttg gaagggggct ggcaatattt
     6901 cgacctgccg cgcaaccccg aatgcccgat ttgcggcgcg gaacggtaaa accctgccgc
     6961 cgtttcagac ggtatccaaa cggatgcggc ggaacggttt taaaaattta aaaatttaca
     7021 tttctttgca aaaaaaaata aacttacctt ataattgcag ttgttttagc aatgtctgtt
     7081 tcgcagactc attgagtaag acgttttccc cgtaatgtgt ttggccgtct gtcccctttg
     7141 ggttcggacg gcttttttta ggctgtgttt gaatacccgg ttggtttcgt ctgtttgcag
     7201 gggaaaaccg cttatttccg ttcgggcgga aaacggttcc atcggataaa aggcattttg
     7261 tccgactgat taaagttaca tcttaggttt gcatcggcaa aatattcaaa cacagccttt
     7321 tttaaggaaa tccggatacg gcggcacatc aataatgcgg cggaatctcg tcgcgcaggg
     7381 aatacggctc ttgcgtgtcg ggattcctgt cctgcatttt ttgatacagc agcctcaact
     7441 gagcctgctg caaatccagc gtctgccgca attccgccac catcgcgttc aggccggcga
     7501 ttacgtcctc ctgaagcgcg gattggattt ccagctctgt gatgcggtgt tccaactctt
     7561 gaaccgcgtc catttacagc accatcgcgg caatccagcc ggcaatcagc agcgggatat
     7621 tgtagtggat gaaggtcggg ataacggaat cgcggatgtg gtcgtgctgc ccgtcggcgt
     7681 tcagccccat cgtcgggccc agcgtggaat cggacgcagg cgaaccggca tcgcccaacg
     7741 cccccgccgt gccgacaatg gcgacggtgg caagcggcga aaaacccaaa ccgacacaca
     7801 aaggcacata aatcgcggca ataatcggca aagtggaaaa ggacgaaccg atgcccatcg
     7861 ttaccaaaag ccccaccacc agcatcgcca atgccgccat acctttgctg ttgccgaata
     7921 tcgccatact gctttccacc agcggctgga tatgcccggt cgcattcatc acggcggcaa
     7981 aaccctgcgc ggcaatcata atgaagccga ccatcgccat catcttgata ccttcgccga
     8041 atacgtcgtt tgccttgtcg cggttgatga cccccaacat cataaatacg gcgaaaccga
     8101 gcatcgcgcc caacaccagc gagtcttcat acatcaactg gatggcaaaa catacggcaa
     8161 tggcggcggc ggcggccagg ctgcggtagg cggacggctg cggacggttt gccgcatcgg
     8221 cgttgcccgc cgtatcggca ttgttgcttt ggtacaggcg cggtttgcgg taatggacaa
     8281 acgccagcag gagtccggcc agcattccca acgcgggaat cgccattgcc gccatcacgt
     8341 taatgttttt cacatcaagc tgcggcgcgg cggaatggat gttgcccaac aggatttcgt
     8401 tcaaaaaaat cgcgccgaag ccgtaaggca ggaacatata agtcgtaacc agcccgaaag
     8461 tgatgacgca cgcaatcagg cggcggtcga ttttcaggcg gttgaacacc aaaagcagcg
     8521 gcggaacaat catcgggata aaggcaatgt ggatggggat gatgttctga ctcatcatgc
     8581 ccatcacaag gatgatggaa agcagcagcc atttgaccgc gccctcgccc gaacgcacgc
     8641 tgtcgggcat accgccccgg ttcagtttgc ggacgaccgc gccggcaagc tgctgcggca
     8701 ggccggaatg ggtaatcgcc atcgcaaacg caccgagcat cgcataagaa agcgcaatct
     8761 tcgcgccgcc ttccaaacct ttgttgaaca cggggataat ccccgcctga ctgacctgtc
     8821 ccgccgcatc ggcaatgttt tgcagcggca tacccgccac cgcgccgccg acaaacgcgc
     8881 cgaccgtcag gctcaatacc acgtgcacgc gcgacagcga cagcaccagc ataacgatta
     8941 cggcaactac gactgcattc attgtttatc gctccaaacc tataaatgtt tacatatcga
     9001 aacacatcat aacccaataa cggaaaaccc gccaattttg caaacaatta tttcaaatgc
     9061 ttcatatact tccccagcgt aaccctgtcc aaacccgcca agtccggcag ggtttccact
     9121 cccgaaaaac cattctccgc caacacgccg cgcaccgccg cgccctgatc gaaaccgtgt
     9181 tccagcaata aaaaaccgcc ttccgccaaa cggtcgggcg cgccttgcgc caaggtacgg
     9241 atgcagctta ggccgtctga aaagtcggtc agcgcgattt gcggctcaaa ccgcaaatcg
     9301 ccttgcgaca aatgtttatc gccgttttcg atatagggcg ggttggacac gatgatgtcc
     9361 catttccctt cagacggcat atcggtgtcg aaccacgaac cgtgtgcaaa ttcgacccgc
     9421 gcgcccaaat ccgccgcatt tttccgcgcc gtttcaaggg cgggcgggct gatgtcggat
     9481 gcgcgcacaa acgcatcggg gcgttcgagc gcgacggtta cggcaaccgc gccgctgccc
     9541 gtccccaaat cccacacgcg cccgttttcg ggcaggcgcg ccaatacggc ttcgaccaag
     9601 tgttcggttt cggggcgcgg aatcagcacg ctcggattga ctgtaaagcg tctgccataa
     9661 aattcgcgca cacctaaaat ataggcaacc ggctcgccgt tcagacggcg ttgcgccagc
     9721 ctgtccgccc gctgtcggac ttcgtccggc atttcttccc cgccccgcgt caacaactgc
     9781 acgcgcgtat attccgaaac atattgcagc agcattcttg cttcattttt aggcagtttt
     9841 gacaagccca accatttatc aaacgtcatt tttatcccgt ctgccgctga tgcggctttt
     9901 ctttccttat tctttccggc aaacgtaccg atggtggcaa ccgcaaatgc ggcataccac
     9961 aaataaaatc ctgcaccgta gcgcacaata tccgatgtat tccctgcttc atcgacgtat
    10021 acggctttca cactgaaagc caccaacgcc aagccccaaa gtgccgcatg gacaggcacg
    10081 accttcttcc gcaacgccag caaaacaatg gccgccaacc aaacataatt cgcatagacc
    10141 gcacaatacc tgatatccaa agaagcaaat atcgacccca aaatcaaaac ggtcaaaccc
    10201 tccatgcttc catgattgcc caaataaaat gcaacattgg ataaagacgc tatccacagg
    10261 gcaaccgaca ccagcaacat cactatggga aaacttgatt tccgattctg ttcctgcatg
    10321 gttttatcct aatgtaaaag gccgcctgaa aacctttcag acggcatcgt gccggattcc
    10381 gcgtcagatt gcgctgccgc cgacggtcag cccggcatcg atacgcaggg tcggctgccc
    10441 cacgccgacg gggacgctct gcccttcttt gccgcacacg ccgacaccgc tgtccaacgc
    10501 ggtatcgttg cctatcatcg aaacgtgttt cagcacttcg ggcccgttgc cgataatggt
    10561 cgcgcctttg acggggtatt gcagcctgcc gccttccacc caccatgctt cggacgcgct
    10621 gaacacaaat ttgccgctgg taatgtccac ctgtccgccg ccaaagttga cggcgtaaat
    10681 gcctttgtcg atggacgcga tgatttcttc cggctcatag ctgccatttt ccataaaggt
    10741 attggtcatg cgcggcatcg ggacggaggc gtagctttcg cggcggccgt tgccggtgga
    10801 ttgcgtaccc gtcaggcggg cattggtttc gtcctgcata tagccgacta aaatgccgtc
    10861 ttcaatcaat acggtgcggc gggtttcgtt gccttcgtcg tcgatgttga gcgaaccgcg
    10921 ccggccggca atatcgccct gatcgacgac ggtaacgcct ttggcggcga cgcgctcgcc
    10981 tattctgccg gaaaagacgc tggtcccctt gcggttgaaa tcgccttcca aaccgtgtcc
    11041 gaccgcttcg tgcagcaaca cgcccggcca gccgttgccc aaaacgacgg tcatttcgcc
    11101 agcgggcgcg gggcgggatt cgaggttggt gagtgcctgt ttgacggcgg catcgacaaa
    11161 ctgccgcacc aagttttcat cgaaataagc caagtcgtag cgtccgccgc cgcccgcgct
    11221 gccctgttcg cggcgttcgc cctgtttggc gataacggta acgttcaggc gcaccatcgg
    11281 gcggatgtcg gcggcgtgtt tgccgtccag acgggcgagg taaaccatat cgtattcgca
    11341 ggtcaaaccg gccatcactt gcacgatgcg cggatcggcg gctttggcga ttgcttccac
    11401 tttgttcaac agcgcgactt tggcggcgga atcgaggccg gcaatggggt cggacgcgga
    11461 acaaaccggc ttgccgcgcg tttcagacgg cattttggcg gacaccttgc cgcctgccgc
    11521 cccaatcgcg cggacggcgc gggcggaacg gtttatcgaa tcgatgcaca ggctgtcggc
    11581 gtaggcaaag gcggttttat cgcccgaaac ggcgcgcacg cccacgccct gattgatttg
    11641 gaagctgccc gatttgacga tgccctcttc caaatgccag ctttcataag cggtgcgctg
    11701 gcagtagatg tcggcataat cgacgtggtg cgcgccgatg atgcacaggc ttttggcgag
    11761 cagttcgggg gaaaggcggt tggcttcgag cagccgctct cgtacggcgg aataggtagg
    11821 atgcatagtg tcggcgcata aaaaatcagg ggcttgatta tacggcattt g
//
LOCUS       DAXHTR010000062        11866 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.062, whole genome shotgun sequence.
ACCESSION   DAXHTR010000062 DAXHTR010000000
VERSION     DAXHTR010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11866
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.062"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            84..445
                     /locus_tag="ACFNML_001135"
                     /pseudo
     CDS             84..445
                     /locus_tag="ACFNML_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239093.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            442..1098
                     /locus_tag="ACFNML_001136"
     CDS             442..1098
                     /locus_tag="ACFNML_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HFC6392530.1"
                     /translation="MILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANAWTK
                     IVRREIIIKNNLFFPKGYIHEDFPYSLQLARFIKTFAFYDNPFYQYRVLGGSISHNIK
                     YKNFSDVLTHLDRGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNIILIY
                     RKYFSFKEKCRKIFGAKTIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKFFSE
                     "
     gene            complement(1077..1211)
                     /locus_tag="ACFNML_001137"
     CDS             complement(1077..1211)
                     /locus_tag="ACFNML_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392531.1"
                     /translation="MPSEAGFVASDGIAAILAGRGLFVPTSRALLIWGLPEAYSEKNF
                     "
     gene            1320..2657
                     /gene="ribBA"
                     /locus_tag="ACFNML_001138"
     CDS             1320..2657
                     /gene="ribBA"
                     /locus_tag="ACFNML_001138"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HFC6392532.1"
                     /translation="MTDTAGLRRRNLRQWIAEHYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPPEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERIQQAESGVVILLHRTEDGAALLDRTLPKGA
                     NQAYKWDSKSYGIGAQILVGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(2817..2933)
                     /locus_tag="ACFNML_001139"
     CDS             complement(2817..2933)
                     /locus_tag="ACFNML_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HFC6392533.1"
                     /translation="MPSERLSDGILFGLENTYAVLSLKERIQKTKATGIYRK"
     gene            complement(2965..4566)
                     /locus_tag="ACFNML_001140"
     CDS             complement(2965..4566)
                     /locus_tag="ACFNML_001140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC6392534.1"
                     /translation="MTEQHFTEQTKSLIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKQIRKENPDEPIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCHALISKLAGFSFEA
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPKEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPAHKDTSGRLKKFDFIVSNPPFKLDFSDFREQLEGEENRERFFAGIPKIKAKD
                     TDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSSIDKKIREYLVENKMLAGMVS
                     MPSNIFATTGTNVSILFIDKANKDNVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVVGYDEIKAKNYSLSAGQYFEVKIDYVDISADEFAQKMAGFSADL
                     DKLFAESAKLEKEIKDRLAMLEFNS"
     gene            complement(4614..5807)
                     /locus_tag="ACFNML_001141"
     CDS             complement(4614..5807)
                     /locus_tag="ACFNML_001141"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6392535.1"
                     /translation="MKRQITIGEIAEINANSLTQKDMFQEIMYLDTGNITRNEIDNIQ
                     VLNVMMDKIPSRAKRKVKDKTIIYSTVRPNQEHYGFLENPGDNFIISTGFSTIDVYDE
                     NTDEKFIYYLLTQKHITDYLHTIGENSVSSYPSINPDDIANLKFAVPDLKTQQSIAAV
                     LSALDKKIALNKQINARLEDMAKTLYDYWFVQFDFPDANGKPYKSSGGEIVFDETLKR
                     EIPKGWGSIELQSCLAKIPNTTKILNKDIKDFGKYPVVDQSQDFICGFTNDEKSILNP
                     QDAHIIFGDHTRIVKLVNFQYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYA
                     RHFKFLKEFKIILPSKDISQKYNEIANTFFVKVRNNIKQNHQLTQLRDFLLPMLMNGQ
                     VSVAE"
     gene            complement(5934..8939)
                     /locus_tag="ACFNML_001142"
     CDS             complement(5934..8939)
                     /locus_tag="ACFNML_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HFC6392536.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREAISSQYK
                     AQLQAALAQLEVEKGSFDRKEIYAHPHFVRPMLDYILGDFAKFRKTNQDESLGAMVVC
                     DSAEQARQLFEHFQTASDHNFTAALILHDVGTKDERDQWVKDFKAGKTDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSQLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIEDKK
                     QLLALKKALQTSKELYNALRLQGSHEFLVHLDFDKLNLLYRETAARLDTLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQDDPEYISLR
                     EELERIFKKKNLAEVGQEEMQANIATLQIVYAKIKELNRQNDLLRHKYGGDAKYARTH
                     KRLMENAAFYGDKLKVFNALNGVKADADQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPQDIQAINRLLVREYLKESGRM"
     gene            complement(9053..10363)
                     /locus_tag="ACFNML_001143"
     CDS             complement(9053..10363)
                     /locus_tag="ACFNML_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HFC6392537.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLARILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRHFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            10675..11096
                     /locus_tag="ACFNML_001144"
                     /pseudo
     CDS             10675..11096
                     /locus_tag="ACFNML_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11784..11866
                     /locus_tag="ACFNML_001145"
                     /pseudo
     CDS             11784..11866
                     /locus_tag="ACFNML_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
BASE COUNT         3012 a         2766 c         2814 g         3274 t
ORIGIN      
        1 gttacagggt gcggatgcaa acgcattgcg agcgcgggtt tgaggcatac gcgcaaacat
       61 cttaatataa tggattgata tttatgattt tctccatcat cgtccctatt tacaatgtgg
      121 aaaaatacct ccgctgctgc gtggattccg tgcttgccga aaattttgcc gattatgaaa
      181 tgattttggt cgatgacggt tcgccggacg gctgcgggaa gatttgcgac gaatatgcag
      241 gcaaatatcc gcatataaaa gtgattcatc aagaaaacgg cgggctgtcg gatgcccgca
      301 acgccggtat ccgggcggca aaaggcgatt acctaatctt tttggacagc gacgactatt
      361 gggccgatac caaccgttca aaaaacgcgg ggggggggga ttctctttga tttacaacaa
      421 cttgcagaca aaaaggttga tttgatcctg catccctcgt ccctcaatta ccacgtcatc
      481 cccaaagggg cggacttttc ggataatgat tttgtccgcc attttgaaac gctggtggag
      541 gggcggtact atatcgccaa cgcgtggaca aaaatcgtca ggcgggaaat aatcatcaaa
      601 aacaatctgt ttttcccaaa aggatacatt cacgaggatt tcccgtacag tttgcaattg
      661 gcgcgtttta tcaagacttt tgccttttac gataaccctt tttaccagta ccgcgttctc
      721 ggcggctcta tcagccacaa catcaaatac aaaaatttca gcgatgtgct gacgcatctc
      781 gaccggggtg tggatttttt agtcgaaaac aaaaattccc ccatctacgg cggtttgcaa
      841 aaatttgtct tcgacaatat cggctatctg aggtctatat tggtaaggct ttatttttcc
      901 aaaaacatta tcctcatcta ccggaaatat ttttcattta aagaaaaatg cagaaagata
      961 ttcggcgcga agacaatccg tccggttttt atcgggaaga ccgcattcat cataggattg
     1021 ccgatattgc gcctgctcgt accgcctatg ctgtacccgg caatcaaggc cgtttatcag
     1081 aaattttttt cggaataagc ttccggcaaa ccccaaatca gaagtgcgcg ggaagttggg
     1141 acaaacagcc cccggccggc aaggattgcg gcaatgccgt ctgaagccac gaatccggct
     1201 tcagacggca tctgtttacc aaaaagcaaa taattcggtt tggcgaaaaa aacagatttg
     1261 ctttttggta aatacgcgat tacaatccgc tacatccgat ttctacaaag gatgaaacga
     1321 tgaccgacac agccggtctg cgccgccgca acctgcggca atggatagca gaacactacg
     1381 gcggtttgca aacccgtttt gccgaagccg ttgccctcaa cacaggcgaa ctctccgccc
     1441 ttttgaaaaa caaatccttc ggcgagaaaa aagcccgtaa aatcgaacag gcggcaaaaa
     1501 tgcccgcctt ctggctcgat accgaacaca ccgcccgccc gcccgaacac acaggaaaac
     1561 acaccatgtc ccatatttcc cccatccccg aaatcctagc cgacatcaaa gccggcaaaa
     1621 tggtcatcat caccgatgcc gaagaccgag aaaacgaagg cgacctgctg atggcggcgc
     1681 aattcgtcac gcccgaagcc atcaacttca tgatcaaaca cgcgcgcggc ttggtctgcc
     1741 tgccgatgga cggcgaaatg gtcgaaaaac tcgggctgcc gatgatgacc caaaaaaacg
     1801 gcgcgcaata cggcaccaac tttaccgtct ccatcgaagc cgcacacggc attaccaccg
     1861 gcatttccgc cgccgaccgc gccctgacta ttcaaaccgc cgtttccccg accgctaaac
     1921 ccgaagacat cgtccaaccc ggtcatatct ttccgcttcg cgcccaaaaa ggcggcgtac
     1981 tcgtccgcgc cggacacacc gaagccggcg tcgacctggc gcaaatgaac gggctgattc
     2041 ctgcctccgt tatttgcgaa atcatcaacg acgacggcac gatggcgcgt atgcccgaac
     2101 tgatgaaatt cgccgaagaa cacaagctca aaatcggcac gattgccgac ctcatcgaat
     2161 accgcagccg taccgaaagc ctgcttgaag acatgggcaa tgcgcctgta caaaccccgt
     2221 ggggcgagtt ccaacaacac gtttacgtcg acaaactctc cggcgaaacc cacctcgccc
     2281 tcgtcaaagg cacgccctcc gccgacaccg aaaccctcgt ccgcgtccac gaacccttca
     2341 gcgtcatgga cttcatccaa gccaacccgc gccattcatg gtcgctgccc aaagcactcg
     2401 agcgcatcca acaggccgaa agcggcgtag tcatccttct gcaccgcacc gaagacggcg
     2461 ccgccctgct cgaccgcacc ctgcccaaag gtgcaaacca agcctacaaa tgggacagca
     2521 aaagctacgg catcggcgca caaatcctcg tcggcctcaa cgtcaaaaaa ctgcgcgtcc
     2581 tcgggcagcc ctcatctttc accggcctga ccggcttcgg tttggaagtc gtcggctttg
     2641 aagaagcgga aaaataatat agtaaattca aatactttat atttgcttta tttattgcat
     2701 tatttccgtg caaacgaaaa cccggtctgt tgggttggat tttgtttttg caaatttcgg
     2761 gtaacttcta attcgtcatt cccgcgcagg cgggaatccg gttcgtcggg tttttgtcat
     2821 ttccgataaa ttcctgtggc tttggttttt tggattctct ctttcaggga aagaacggca
     2881 taagtatttt ccaaaccaaa caaaatgccg tctgaaagcc tttcagacgg cattttaagt
     2941 ttgaccggtt tcattcatcg gtatttacga attgaattcc aacattgcca gcctatcctt
     3001 aatctccttt tccaatttgg cagattcggc gaaaagttta tccaaatccg ctgaaaatcc
     3061 cgccattttt tgcgcaaatt cgtcggcgga aatatccaca taatcaatct ttacctcgaa
     3121 atactgcccc gccgacaggc tgtaattctt ggctttgatt tcatcgtagc cgaccaccac
     3181 gctgaaatct tccactgcct gtttgtgcgt gaaagtattg caaatttttt gttcttcttc
     3241 gcgggagagt acggtttttt ggtttttgcc gtctttgatt ttttcgccca agcccgatgc
     3301 gtcgattaat accacgttgt ctttattggc tttatcgata aacaggatgg aaacgttggt
     3361 tcccgtggtg gcgaaaatat tggaaggcat ggaaaccatg ccggcgagca ttttgttttc
     3421 taccagatat tcgcggattt ttttgtcgat actcgattgg gcggtgataa agcccgtcgg
     3481 caacacaatc gccgctttgc cgttttcttt caggctaaac aagatatgct ggataaaaag
     3541 ctggtaaatt tccatcttat ccgtatcttt tgccttgatt tttgggatac cggcaaaaaa
     3601 gcgttcgcgg ttttcctcgc cttccaattg ctcgcgaaaa tcgctgaaat ccaatttaaa
     3661 cggtggattg gaaacaatga aatcaaattt tttcagacga cctgaagtgt ctttatgggc
     3721 gggcgacaaa atcgtattac cctgaaccac attattcaaa gaatgcacca aattgttcaa
     3781 aatcagattc agccgcaaaa gattggacga tttttgcgaa atatcctgcg tataaatcat
     3841 acatttgtct tcaccgatgg cgtgcgccac gttcatcagc agcgttcctg aaccggctga
     3901 cggatcgtaa acgtccacgc tgcggatttg cccacgtacc tcctttggca ccaaaatatc
     3961 cgccataatc cgcgctactg cgtgcggcgt gtagtattcg gcatatttgc cgcccgaatt
     4021 actgttgtag tctttaatca ggtactcaaa aatcgtggcg aaaaaatcaa atttttgcgc
     4081 aaaaatcgcc tcgaagctga aacccgccag tttggaaatc agcgcgtggc aaaaatcgtc
     4141 gcggcgacct tcatccgtaa tatattgact gatacgctcg aacaacacga ttttcgcccc
     4201 gccttcggtt tttaccgaaa acagttccgc attatgtgcg gcaatatcgg tcagcgtatc
     4261 gtcaaagagt ttggcaaagt ccgcgccgtt ttgtcgctct gataaatact tgatggaatg
     4321 ttcaggcttc aaaacggctg tttttccctc aatatccata ttgatgaatt cgattggctc
     4381 gtcgggattt tctttgcgaa tttgtttaac ttcgaaatcg tatttgtcgt ttaggaattt
     4441 atacaaaaat gcctgactga tgattttgaa ctcgttgccg tcattgccca aaccatagtt
     4501 ggcgcaaatg gtttttaagc tgtcgattag ggatttggtt tgttcggtaa agtgttgttc
     4561 ggtcattttt ttctcgtttg ttttattttg taggtctgat tcttgaattt ggcttattcc
     4621 gccacagaaa cctgcccgtt catcagcatg ggaagtagga aatcgcggag ttgggttagt
     4681 tggtggtttt gttttatatt atttcgaact ttaacaaaga aagtatttgc tatttcatta
     4741 tatttttgag aaatatcttt actaggtaaa attattttaa attcttttaa gaatttgaaa
     4801 tgtctagcat atccataact tgataaatct atttgattta tgatttggta aaacaaatag
     4861 tttggcattc gttcattatt gcttaatatt acttgtgtac catctgctcc ccgcgcatat
     4921 tgaaaattaa ctaacttaac aattcttgta tgatcaccaa aaataatatg agcatcttgc
     4981 gggtttaata tagatttttc atcattggta aatccacaaa taaaatcttg actttggtca
     5041 acaacaggat attttccaaa atccttaata tctttattta atatttttgt agtatttggg
     5101 atttttgcta agcaagattg taattctata cttccccacc ccttcggaat ttcccgtttc
     5161 aaggtttcat caaacacaat ttcgccgcct gaggatttgt agggcttgcc gttggcgtca
     5221 gggaagtcga actgcacgaa ccaatagtcg tacagggttt tcgccatatc ttccaggcgc
     5281 gcattgattt gtttattcag ggcgattttt ttgtccaaag cggataggac ggcggcgatg
     5341 gattgttggg ttttgaggtc aggaactgca aattttagat ttgcaatatc atcaggattt
     5401 atcgatggat atgatgaaac actgttctcg ccaattgtat gtaaataatc agtgatatgc
     5461 ttttgagtca ataaatagta aataaatttt tcatccgtat tctcatcata aacatcaatt
     5521 gtactaaacc ctgttgatat aatgaaatta tcaccaggat tttccaaaaa accgtaatgc
     5581 tcctgatttg ggcgaactgt agaatagata atagtcttat cttttacttt tctttttgca
     5641 cggctaggta ttttatccat catgacattc aaaacttgga tattatctat ttcatttcta
     5701 gtaatattgc cagtatccaa atacatgatt tcttgaaaca tgtctttttg agtgagggag
     5761 ttggcattta tttctgcgat ttcaccaatt gtaatttgtc gtttcatctt aacttaatct
     5821 ctcgaaactc ttcaaaggcc gtctgaaaaa aatttcaaaa tccaaccgca cttttattgt
     5881 ccacttcgaa cgtatgcaat acgcccataa aggctaccta aaaaaccgct ttctcacatc
     5941 cgcccgcttt cttttaaata ttcccgcacc aacaggcggt tgatggcttg gatgtcttgg
     6001 ggttgaacag gaaattgttg ctccgttcta aagcgtttta atacgatgcc ctgcatttgt
     6061 ttttcaaagt aattttgatt gtccaaaatc tgctccatat ccagcacttt ttggtcggca
     6121 tcggctttga cgccgtttag agcgttaaaa actttgagtt tgtcgccgta gaaggcggcg
     6181 ttttccatca gccgcttgtg ggtgcgggca tacttagcgt cgccgccgta tttgtgccgc
     6241 aaaaggtcgt tttggcggtt cagctctttg attttggcat agacgatttg gagtgtggcg
     6301 atgtttgcct gcatttcttc ttgcccgact tcggcgaggt tctttttctt gaagatgcgt
     6361 tccagctctt cgcgcaggct gatgtattcg gggtcgtctt ggtcgaagtt gcccgccagc
     6421 ccttcacgga ctttgcgcat gatgtctttt aaatcgtcgg caagtttgag ttccgcctcg
     6481 ccgattttga cgaattgaaa gtaaacgtct tccagcgctt cattgagcaa atgggcggtg
     6541 tcgccttgct gtaatttttc ggcaaggttt aaggtatcca gacgggcggc agtttcgcgg
     6601 tacagaaggt tgagcttgtc aaaatccaga tgcacaagaa attcgtggct gccttgcagg
     6661 cgcagagcgt tgtatagctc tttggaagtt tgcagggctt tttttagggc gagaagctgt
     6721 tttttgtctt ctatttggct aatttggctg caaaattctt cggcgttttc ggtatcgaaa
     6781 tcaaacaggg cgtttttaat gtctgcaatt tcctgttcga tttcttcggc ggttttgaat
     6841 agctggctgt aactgccgat ttcgtcgccc aattcgttag aaagttcgtc ccaataggcg
     6901 cggttggttt tgtcaaattc gcgttcgatg tcggcaaaat cgacaacata gccgtaacgg
     6961 taagatttgt aggtgcggtt gacgcgggtc agggtttgta aaaggttatg ggctttgatg
     7021 agtctgccca aatagagctt tttcaggcgc ggcgcgtcaa agccggtcaa aagcatgttg
     7081 tacacaaaca ggatgtcggt tttgcctgct ttgaaatctt tgacccattg gtcgcgttcg
     7141 tctttggtgc cgacgtcgtg caatatcagt gcggcggtga aattatggtc tgaggccgtc
     7201 tgaaaatgct caaaaagctg gcgcgcctgt tctgcgctat cgcaaacgac catggcgccg
     7261 aggctctcgt cttggttggt ttttctgaat ttggcgaagt cgcccaagat gtaatcgagc
     7321 atcgggcgga cgaaatgcgg atgggcgtag atttctttgc ggtcaaagct gcctttttcg
     7381 acttcaagct gcgccagtgc agcctgcaat tgggctttgt attggctgct gatggcttcg
     7441 cgaatcaggc gcagggtgta gccgtcggcg atggaggcgt tgtaatagta tttatggatg
     7501 tagtcgccga agagttcgcg ggtattgacg ttgcctgcgg ttacgccgat aagcggcgtg
     7561 ccagtcagcc cgattttgac ggcgtttacg tcggactggt tgaggttggc aagaaacgag
     7621 cctttggggt tgtagctgcg gtgtacttcg tcgagaaaat agacgcgctg aatggcgagg
     7681 tcgtagtcgt tacgagcgac gacatcgggg tcgtcttgga atttttggat attaacaaca
     7741 gtgatttccg ctttgcctgc gtggttgtgc aaagtttggg cggatttgat gtcggcagca
     7801 aaggcttcgc ggctgtcgat ggtatggacg actaaatcgc gggcagtaaa ttcgcgctgc
     7861 gcctgtttca ataaatcaag cctgtccacg atgaaataga atttcggcac gatgccctgt
     7921 ttggcataat agtgggtcag gtagcgggtg ttgtaatagg caagcgcggt tttgccagag
     7981 ccttgggtgt gccaaatcac gccttttttg ccgccgtttg ccaaatgttt ttcaatggcg
     8041 agagtggcaa atagctgcgg ataccgcatg atgtgttttt gcaccaagcc ttgacttgct
     8101 ttgacgtaag tcagcccgtg ttgcagaaga aaagaaagac gttcgcggca aagcagcgaa
     8161 gtcaggatgc ggttggtcgg cgtatcgggc gatttattgc tgataaattc ggggctgtgt
     8221 ttgataacgg gcaggttgtt gtcttgcagg acgttgtttt cgaggccgtc tgaaagcgtg
     8281 ttcaataatt cggcaagatt taatttatgt tcttcgcgga agtagttaaa cacgggctta
     8341 ccgtaagcag acgaagcgta aaacgcgcct tgtgccggtt cggttgcgcc gtcgtcgtat
     8401 tccatgttgt tggaaaaaat catgaattgg gtgatgttga tgaaacggcg aaatttggaa
     8461 tttttcgcac gtttgcccat gcgctcccgc tcttcgccga ttccgccttt gttgttgggc
     8521 tttttgactt cgataaaaac caaaggcatg ccgtttacca gcagggtaat gtcggggcgg
     8581 aattcttcat cgccgttgat acagggcaat tcggttacaa cgtggaagct gttgttatca
     8641 aaattttgga agtcgatcag tttcttgccg ccggattgat tggtaaggcg ttcgtaaaac
     8701 ttttgcccca agtcttcgtt gtccagttct aagcgtatgt cggtcagcaa acggcgtgca
     8761 tcgtccggcg acaaatcggg attgatgcgg cagaggcttt ctataaagat tttgggaaag
     8821 atgttggttg gtttatccca attttcgttt ttgagcgaga ggtagtcgta gcccaagcgc
     8881 atcaggtgca aaacggcggg gattttgacg cgggaatttt cgttgtgagt ttgggacata
     8941 aacgtaatga ttcatcaaaa tttattgaaa cggattataa cacctgaaaa acccgatacg
     9001 cctttttggt gtaccggctt tgccataccg ttctgcttca gacggcattg cttcattttg
     9061 cctttaatgc ctcttcatcc agcgatttga gccacgccag cttttcgccg attttgattt
     9121 ccaacccgcg cgggacgggt tggtagaagt ccggttcgtc caagccgtcg ggcatatagc
     9181 tttcgccggc ggcgtaggcg ttcggttcgt cgtgggcgta gcggtattcg cgtccgtagc
     9241 ccaattcctt catcagcttg gtcggggcgt tgcgcaggtg gacgggcact tcgtcgctag
     9301 cgttttcttt gacgaagtgg cgcatttggt tgtatgcctt gtagcccgcg ttggatttcg
     9361 cggcggcggc aagatacaat accgcttgcg ccaaagccag ttcgccttcg ggcgagccta
     9421 agcgttcgaa ggtggcggcg gcatcgttgg cgatttggaa ggcgcgcggg tcggcaagcc
     9481 cgatgtcttc ccaagcgata cgcacgatgc ggcgggcgag gtagcgcggg tcggtgccgc
     9541 cgtcgagcat acggcagaac caatacagcg cggcgttcgg atgcgaaccg cgcacggatt
     9601 tgtgcagggc ggagatttgg ttgtagaaac tctcgccgcc tttgtcgaaa cggcggattt
     9661 gcgccccgag actgtcggcg agaaattcgg cggttaagtt tttcagacga cgtgtatcgg
     9721 cggcgcgtaa aagttgttcc aacaaattca acaatctgcg cgcatcaccg tcggcggtat
     9781 tcacgagtaa tttttgcgca tccgtttcaa tcgtaaactc ttggtattca ggcaaagcca
     9841 ataccttggc aatcagcttt ttcaggtcgt ctgaagacaa gggttgcaaa acatacacct
     9901 gagcgcggct caacagcgcg ggattgactt caaacgacgg attttccgtc gtcgcaccaa
     9961 taaaggttag caaaccgctt tcgacatgcg gcaaaaacgc gtcctgctgc gccttgttga
    10021 agcggtggac ttcatcgaca aacaaaatcg tcgcgcgtcc ctgctgcaaa gcgatttcgg
    10081 ctttatcgat tgcctcgcgg atgtccttca cgccggaaaa tacggcggaa acaggcaaaa
    10141 actgggcgtt gaaactctgc gccaaaatcc gcgccaacgt cgtcttgccc acgcccggcg
    10201 gcccccacag caacatagaa tgcggcttgc cgccttctac cgccacgcgc aaaggtttac
    10261 cttcgccgat gaggtgttcc tgccccacca cgtcgtcaag cgtatgcgga cgcaatcgtt
    10321 cggcaagcgg cgcgtcgggt tctcgggcaa acaaatcggt cataacggct ccgtcaacag
    10381 gttttcaaac aatatgatta tacggcaggg aacggcggcg tgccgcatac ggattccgcc
    10441 cctccgtttg ccttaagccg atattaggcg catactggaa aagacgagag acttcacaca
    10501 atatatccgg cacggagacc gattccgcat cggcatgaca atacccaaat cagcgtttca
    10561 attaaacatt aaggagacta aaatagaaaa tttgctttat ctaccattgc tttgttgatt
    10621 caatcggcat tatgttttga ggcggaagcc catgaatata ctaatattca agagatggaa
    10681 tgggtgtctt tattttctga tccgcaaaga gacgatgata gtcttataac ccttaaagat
    10741 gaaaaaatca ctgtaaaaaa ctatattgtg ccttggtgga aaaaaggtga aaactttaga
    10801 aaattagaac ttattaccac tggtaaaaat gataatggaa atttaacggt aaatggaatt
    10861 tacggcatcc aatcttttgc agactctcaa ttgggatata cgcctgaatt attggtaaaa
    10921 agcaataatg atattctatt taaaaataat ctccaatctg ttgacgtgaa gccccattcc
    10981 ggaaatatcc atttggatgc caaaggaaaa attcaattta atttgaatgt tcccgatgtt
    11041 gatggggtac aagatgtagc cagtgaaaaa aataccgaca aacaatagtg ccttagttcg
    11101 gtatgtcaac actatcagcc ccccgatgaa aatgacaaag gcagcatatc ctttcattca
    11161 aaaacaggca atttgttcaa tatgaacgtt aaagttcata aagacacaca ccatttcggt
    11221 attcacaacc tcaacaatac cgatgttcat ctgactgccg aagacagcga taatgtgatt
    11281 gagattaaag gggaactcgc cgaccctatg ccgacaggaa atgttgagag tacaagtttt
    11341 ttaaggggca ttgaaacggc ttttaaatcc aataccgtat taacggggaa taacaataaa
    11401 atatccatcg ccactgaggg caagggcttg ggattgggcg taaatggtct ccagtccaac
    11461 tccccctatt ccggacacac gacgggcatt ttcatcaata atcaatcaag cgaccttacc
    11521 caaccttccc ttgatggcat cagcaccatc aaactcaaag ccgtcaaggg aaataatgaa
    11581 gttgatttaa atgtaaaaaa ccacgcctcc gtaaaaggca ttatcgcgtc gcattctgca
    11641 aaagcaacgc ttgaagccga aggggacaat attgtccagg ttaaaaatcc ggacaccaat
    11701 acggctttaa tcgatgcttt gaaaaaacaa taccccaatg cttcgaaaga actatacagg
    11761 gtgggggttc aatccacgac tggaacagta cgcatttcgc caaacggcaa aatcgcatta
    11821 acggaattga aaacttttgg aattgtacaa aacggcattt gcgtca
//
LOCUS       DAXHTR010000063        11614 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.063, whole genome shotgun sequence.
ACCESSION   DAXHTR010000063 DAXHTR010000000
VERSION     DAXHTR010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11614
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.063"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            46..285
                     /locus_tag="ACFNML_001146"
     CDS             46..285
                     /locus_tag="ACFNML_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392538.1"
                     /translation="MNVWDIWGRMVEIYHKYKKPCLVLAVDFVMGMVFIEPNEELCIG
                     RCYAPMSESPDFANAVAMAVAMICIVWIAVLSMTD"
     gene            499..1800
                     /gene="bioA"
                     /locus_tag="ACFNML_001147"
     CDS             499..1800
                     /gene="bioA"
                     /locus_tag="ACFNML_001147"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFC6392539.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
     gene            1797..2444
                     /gene="bioD"
                     /locus_tag="ACFNML_001148"
     CDS             1797..2444
                     /gene="bioD"
                     /locus_tag="ACFNML_001148"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFC6392540.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGKPMQEADKQGLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTENLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLSYLKCRLKADFSEAEWMELAKTDAV"
     gene            2411..2641
                     /locus_tag="ACFNML_001149"
     CDS             2411..2641
                     /locus_tag="ACFNML_001149"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392541.1"
                     /translation="MDGVGKNRRGIKIGKNMEHLYDEYGNTTPNRRTTNKKDETISSK
                     VTVAAIPVAAPFAIADIVDQDFIQAIMKITGH"
     gene            2665..3051
                     /locus_tag="ACFNML_001150"
     CDS             2665..3051
                     /locus_tag="ACFNML_001150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392542.1"
                     /translation="MKCYIKVVIIVFIMVMTTSCNPCFFATSSCDYSFRKNYPINSDD
                     EYYVKNNTSKETRIADLRYCISYACPEVYQKYQLPDENGDYREIIRRKYKDDYKKGYK
                     NADDVMTCYGKIGECMKNNKGYSTKW"
     gene            3045..3488
                     /locus_tag="ACFNML_001151"
     CDS             3045..3488
                     /locus_tag="ACFNML_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392543.1"
                     /translation="MVMFRLLSTALVLLIMQGCYYTTIDNEKCFVAPQSVHCPVSSDI
                     ENVIRKNPNKSINQVRSDIKYCANMYFSNIRELNIPQDFDNYIFMLKEKGIPRNDKEL
                     AYNAVRNMHIICLSSLGGYDYYQKTAEFQKIDYKKEMEKLLNENH"
     gene            3559..3942
                     /locus_tag="ACFNML_001152"
     CDS             3559..3942
                     /locus_tag="ACFNML_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392544.1"
                     /translation="MITRCFKVVIIVFIMVMTTSCNPCFFATSSCNYPFDDMTIRANN
                     DIEYFVKNNTTNEQRIDDFRSCLSVACPEIYKLYQLPDEKGNYQYIIKKYYDNKYNDN
                     AVKSTNCIGDNARCMLNNKGYSTKW"
     gene            3945..4421
                     /locus_tag="ACFNML_001153"
     CDS             3945..4421
                     /locus_tag="ACFNML_001153"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFC6392545.1"
                     /translation="MGYGFEEWLPDLPAPVSDGIGLPMSRLLKARSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            4451..5341
                     /gene="ubiA"
                     /locus_tag="ACFNML_001154"
     CDS             4451..5341
                     /gene="ubiA"
                     /locus_tag="ACFNML_001154"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFC6392546.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVGKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFTAIFAHTFFAK"
     gene            5526..5975
                     /gene="ptsN"
                     /locus_tag="ACFNML_001155"
     CDS             5526..5975
                     /gene="ptsN"
                     /locus_tag="ACFNML_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFC6392547.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLSHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVKQATGAFIRTREPVGFDAPDGKPVSLIFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            5977..6939
                     /gene="hprK"
                     /locus_tag="ACFNML_001156"
     CDS             5977..6939
                     /gene="hprK"
                     /locus_tag="ACFNML_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HFC6392548.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGDLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            6920..7774
                     /gene="rapZ"
                     /locus_tag="ACFNML_001157"
     CDS             6920..7774
                     /gene="rapZ"
                     /locus_tag="ACFNML_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFC6392549.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIASLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIERFVTHWLPRLEDESRSYVTVAIGCTGGQHRSVYIVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            7855..8796
                     /gene="ylqF"
                     /locus_tag="ACFNML_001158"
     CDS             7855..8796
                     /gene="ylqF"
                     /locus_tag="ACFNML_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HFC6392550.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKVWLDHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEESGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGKKPSEA"
     gene            8897..9499
                     /locus_tag="ACFNML_001159"
     CDS             8897..9499
                     /locus_tag="ACFNML_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HFC6392551.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            9683..11356
                     /gene="recN"
                     /locus_tag="ACFNML_001160"
     CDS             9683..11356
                     /gene="recN"
                     /locus_tag="ACFNML_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFC6392552.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRVEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            11466..11542
                     /locus_tag="ACFNML_001161"
     tRNA            11466..11542
                     /locus_tag="ACFNML_001161"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11500..11502,aa:Arg,seq:acg)
BASE COUNT         3005 a         2733 c         3033 g         2843 t
ORIGIN      
        1 ggctgtaacc ttcaatcgga atttgttgcc tgcgggatat gcggtatgaa tgtttgggat
       61 atatggggca ggatggtgga aatctatcat aagtataaga agccgtgcct ggttttggcg
      121 gtggattttg tgatgggtat ggtattcata gagccgaatg aggagctgtg catcggtaga
      181 tgctatgcgc ctatgtcgga gtcccctgat tttgctaacg ctgttgcgat ggctgttgct
      241 atgatctgta tcgtatggat tgccgttttg tctatgactg attgaagcgt tgggcggagg
      301 ctgtgtgaaa caatattggg cgttgggtcg tctgattcca atcgggcttg gggaatgcga
      361 aacggtgtgc gcttatactg cggacgattt gttgcgtggt tttacgcccg aaacggatgg
      421 aaaggtgtgg gaagcggtct gtcggagtag aatacgcgtt ttgcgtttga atgcagcaat
      481 aagaaaagag agaaacttat gccgtctgaa catcaacaca catcatcatt gcttgatttc
      541 gaccgtaccc atctgctcca tccctatact tccatgaccg atccgcttcc cgtttatcct
      601 gtcaaacgtg cagaaggggt gtttatcgaa ttggcggacg gcacgcggct gattgacggg
      661 atgtcgtctt ggtggtgtgc gatacacggt tacaatcatc ccgttttgaa tcaggcggtt
      721 gagacgcaga tgaaacagat gtcgcatgtg atgttcggcg gtttgacgca tgagccggcg
      781 gtggagctgg gcaaattgtt ggtcgggatt ttgccgcagg ggctggatcg tattttttat
      841 gcggattcgg gttcggtttc ggtggaagtc gcgttgaaga tggcggtgca ataccagcag
      901 gcgcggggtt tgcgtgcaaa acaaaatatg gcgacggtgc ggcgcgggta tcacggcgat
      961 acttggaacg cgatgtccgt ctgcgatccg gaaacgggga tgcaccatat tttcggcagc
     1021 gcgttgccgc agcgttattt tgtcgataac ctgacaagcc gtttcgacga tgaatgggac
     1081 gaggcggatt tgcagcctgt ccgcgccttg tttgaagcgc atcatacgga cattgccgcc
     1141 tttattttgg aaccggtcgt gcagggcgcg ggcggtatgt atttttatca tccgcagtat
     1201 cttcgcggat tgcgcgattt gtgcgacgag tttgatatcg ttttgatttt tgatgaaatc
     1261 gccaccggat tcgggcgcac gggcaagatg tttgcctgcg aacacgcgga ggtcgtgccg
     1321 gatattatgt gcatcggcaa gggtttgagc ggcggctata tgacgctggc ggcagcaatc
     1381 acttcgcaaa aagttaccga aacgatttcg cgcggagaag cgggcgtgtt catgcacggc
     1441 ccgacattta tggcgaaccc gctggcgtgt gccgttgcct gcgcttcggt caaactgctt
     1501 ttgtcccaag attggcaggc aaatatccgc cgcatcgaaa gcatcttaaa atgccgtctg
     1561 aaaaccgcgt gggacattcg tggtgtgaaa gacgtgcgcg ttttgggcgc catcggcgtg
     1621 atcgagttgg aaaaaggcgt ggatatggcg cgttttcaag cggattgcgt ggcgcagggc
     1681 atttgggtgc gcccgttcgg caggttggtg tatctgatgc cgccttacat catttcagac
     1741 ggcgttttga ccaaacttgc cgacaaaacc gtgcaaatct tgaaggaaca cagcaaatga
     1801 aaggcgttta cttcgtcagc ggcatagaca cggacatcgg caaaaccgtc gccaccggcg
     1861 tgttggcaaa acaattgttg cagcagggca aaagcgtgat tacgcaaaag cccgtgcaaa
     1921 ccggttgcca aaacattgcc gacgacatcg ccgtccatcg taaaatcatg ggtaaaccca
     1981 tgcaggaagc cgacaaacaa gggctgacca tgcctgaaat cttcagttat ccagcttcgc
     2041 ctcatctcgc tgcccgtctt gacggcaggg ctttggactt ggacaaaatc cgcaccgcca
     2101 cacaagaatt ggcggcgcag tacgaagtcg ttttggtcga aggcgcgggc ggattgatgg
     2161 ttccgctgac ggaaaacctg ttaaccattg attatatccg tcagcaaggc tatcccgtca
     2221 tcctcgttac cagcggacgg cttggcagta tcaaccacac tttactcagt ttcgcagcgc
     2281 tcaaacaata cggcattcgc ttgtacagcc tcgtgttcaa ccacatccac gacagccgcg
     2341 acgcacacat cgcccaagac agcctgagct acttgaaatg ccgtctgaaa gccgattttt
     2401 ccgaagcgga atggatggag ttggcaaaaa cagacgcggt ataaagattg ggaaaaatat
     2461 ggaacaccta tatgatgaat atggaaatac aacgccaaat agaagaacaa caaataaaaa
     2521 agatgaaact atttcaagta aagtaactgt tgcagcgata ccagtagccg ctccatttgc
     2581 tattgctgat atagtcgatc aggattttat ccaagcgatt atgaaaatta ctggtcattg
     2641 attatctata tattggtggt tgttatgaaa tgctatatta aagttgtcat tattgttttt
     2701 ataatggtaa tgacaacttc ttgtaatcct tgcttttttg caacttcttc ttgtgattat
     2761 tcttttagaa aaaactatcc tataaatagc gacgatgaat attatgttaa gaataatacg
     2821 agcaaagaaa caagaattgc ggatttacgc tattgtattt cttatgcatg tccagaagtt
     2881 tatcaaaaat atcaactacc agatgaaaat ggagattata gggagattat tcgtagaaaa
     2941 tataaagatg attataaaaa gggctataag aatgcagatg atgtcatgac atgctacgga
     3001 aagattggag aatgcatgaa aaataataaa ggatattcaa caaaatggta atgttccgct
     3061 tattatcaac agcccttgta ttgttgataa tgcagggctg ttattacaca actattgata
     3121 atgaaaaatg ctttgttgcc ccacaaagcg ttcattgtcc tgtaagttct gatattgaaa
     3181 atgttattag aaaaaatcct aataaaagca taaatcaagt taggagtgat attaaatatt
     3241 gtgctaatat gtatttttcc aatatacggg aattaaatat tccgcaagat tttgataatt
     3301 acattttcat gcttaaagaa aaaggtattc caagaaatga taaggaattg gcttataatg
     3361 cagttagaaa tatgcacatt atttgtttga gttctttggg tggttatgat tattatcaaa
     3421 aaaccgcaga atttcagaaa atagattata aaaaagaaat ggagaaattg ttaaatgaaa
     3481 accattaaat ttgctattgc cgatatagtt gatagcgact ttatccaagt gattatgaaa
     3541 ttaagcgggc attgatttat gattacgcgt tgttttaaag ttgtcattat tgtttttata
     3601 atggtaatga caacttcttg taatccttgc ttttttgcaa cttcgtcttg taattatcct
     3661 tttgatgata tgacgataag agcaaacaac gatattgaat attttgttaa aaataatacg
     3721 actaacgaac aacgaattga tgatttcaga agttgccttt ccgttgcttg tcctgaaata
     3781 tataaattat atcaattacc tgatgaaaag ggtaattatc aatatattat aaaaaaatat
     3841 tatgataata aatacaatga taatgcagtc aaatcgacaa attgtattgg ggataatgca
     3901 agatgtatgc ttaataataa aggatattca acaaaatggt aataatgggt tacggctttg
     3961 aggaatggct gcccgacctg cccgcccctg tttcagacgg catcggcctg ccgatgagcc
     4021 ggttattgaa agcccggtcg ctgaccgccg cattgcgcgc cttgccgcat acattttcgg
     4081 tggaactgct gaaactgggc gaattggaga cggaatgcgg agggaggctg gtgcgcgaag
     4141 ttttgttgaa gctggacggt atccctgttg tcgcggcaag gagcgagtgc cgtatcggtt
     4201 cggcgttttg gcaaaacatt ttggactgcg gcacgcgtcc tttgggcgag cgtctgtttc
     4261 aagccgattt ggaaggggcg cgttcggcgt ttgagtttgc cgtttccggc gaaggatgcg
     4321 gacggtactt tgccgcgcgg cgttctcggt tttcccgtca cggcgaggaa atgctgctga
     4381 ccgagtattt tctgcccgaa ctgaaacgct ttatcggata aaataccgtt ttttcaagct
     4441 gtgcggcaat atgaatccta aatccccttt atttttacga ctgtccgatc gtttggatgt
     4501 gtacctgcgc ctgatgcggg cggacaagcc cattgggacg ctgcttttgc tgtggccgac
     4561 ctactgggca ttgtggctgg cttcagacgg cattcccgat ttggcggtat tggcggcgtt
     4621 tacaatcggc acgtttttaa tgcggagtgc cggttgcgtc atcaacgatt ttgccgaccg
     4681 cgattttgac ggtgctgtcg agcgcaccaa aaaccgtccg ttcgcacagg gtcgggtcgg
     4741 aaaaaaagaa gcgctgctgc tgacggcatt tttgtgcctg cttgccgcat tgtgcctgat
     4801 tccgctgaat catctgacat ggctgatgag cctgcccgcg ctgtttcttg cgctgactta
     4861 cccgtttacc aaacgctttt ttccgattcc ccaactctat ctcgggcttg ccttttcctt
     4921 cggtatcccg atggcgtttg ccgccgttgc cggaaacgtg ccgcctcaag cgtggatact
     4981 ctttgccgcc aatgtgttat ggactctggc gtatgacacg gtttatgcaa tggcggacaa
     5041 agaagacgat ttgaaaatcg gcatcaaaac ctccgccgtc acgttcgggc gttacgacat
     5101 cgccgccgtt atgctgtgtc atggcggctt taccctgctg atggcagtat tgggtgcggt
     5161 tatcggtgcg gcatgggcat attggacggc aatccccatc gtcctgctgc tgcaataccg
     5221 ccaatatgcc gccatcaaaa gccgcgtccg gcaaatctgt tttgaaacgt ttttggcaaa
     5281 caacagaatt ggttgggtgt ggtttaccgc catttttgcc catacgtttt tcgcgaaata
     5341 aggcagggca atgccgtctg aagagccgta aactgctttg gacggcattt ctatctgtgc
     5401 cgaaaagcgt taaaatatgt ttttaaaacg ctgtgttatg tcagcccgta ccgtatgcgg
     5461 gattgagatt tgccctagca gtcggtacaa tctttctgtt ttgcgatgtc tgaaaagaga
     5521 agcttatgag ccttatcggc gaaattttgc ctttgtccca tattgttttg gatatggagg
     5581 tgggcagtaa aaaaaggctg tttgaggaag cgggcctgct tttggaacgc gaatcctcat
     5641 tgtcccatgc tgatgttttc gaatgtcttt ttgcccgtga aaaactcggt tcgaccggtt
     5701 tggggcaggg cgttgccatc ccgcacggcc gtcatgccgg cgtgaagcag gcgacgggcg
     5761 cgttcatccg cacgcgcgaa cccgtcggat ttgacgcacc ggacggcaag ccggtttccc
     5821 tgatttttat cttgctggtg ccggaaaacg caactggcga acatttggaa gtcttatcca
     5881 aactggccgg caagttttcc caaaaaagca tccgcgaatc gctgatgacg gtttcttctg
     5941 cggaagaagc ccgcgccatt ttgactgaag aataatatgc ccagtatttc cgtccgccga
     6001 ctgtttgacg acaaccaata caagttacaa cttgcctggg ccgccggcaa ttcgggtgcg
     6061 gacaaccgta tcggcgtaga ggcggacaag cccgtcctcg ctttggtcgg acacctgaat
     6121 ttcatccacc ccaaccaaat ccaagtggtc ggtttggcgg aatcggaata tctgaaccgt
     6181 ctcgaatcgg gggaaacggg ttatcagttc ggcgacctgt tcgacatttc tatgtctttg
     6241 gttattgtgg caaacggctt gccggtttcc ccggggctgc gcgactattg ccataaaaac
     6301 gatattccac tgctgacttc caaactcgaa agcccctatc tgatggacgt gttgcggatt
     6361 tacctgcaac gcaccctggc ggcatcgtcc gtcaaacacg gcgtatttct cgatgtgttt
     6421 gaaatcggcg tgctgattac cggacattcc ggcttgggca agagcgaatt ggcgttggaa
     6481 ctgatttcgc gcggccacag cctgattgcc gacgatgcgg tcgagctgtt ccgcatcggt
     6541 ccggaaacat tggaagggcg ttgttcgcct atgctgcgtg attttttgga agtgcgcggc
     6601 ttggggatac tcaatatccg ccatattttc ggcgaaactt ccatccgccc caaaaaaatc
     6661 ctgcaactca ttatcaattt ggtcgaggca gacgacgagt atatgaagca gctcgaccgg
     6721 ttgagtatcc gcaccgaaac cgaatccatc ctcaacgtca acgtccgttc ggttacgctg
     6781 cccgtcgccg tcggacgcaa cctcgccgtt ttggttgagg cggcggtacg caattacatt
     6841 ttgcagttgc gcggtaagga cagtacgcgc gaatttttgg aacgccatca gacgcaactt
     6901 aaagaaaacg aacagaatca tgaaaatcgt cctgattagc gggttgtccg gttcgggcaa
     6961 atccgtcgca ctgcgccaaa tggaagattc gggttatttc tgcgtggaca acctgccctt
     7021 ggaaatgttg cccgcgctgg tgtcgtatca tatcgagcgt gcggacgaaa ccgaattggc
     7081 ggtcagcgtc gatgtacgtt ccggcattga catcggacag gcgcgggaac agattgcctc
     7141 tctgcgcgga ctggggcaca gggttgaagt tttgtttgtc gaggcggaag aaagcgtgtt
     7201 ggtccgccgg ttttccgaaa ccaggcgagg acatcctctg agcaatcagg atatgaccct
     7261 gttggaaagc ttaaagaaag aacgggaatg gctgttcccg cttaaagaaa tcgcctattg
     7321 tatcgacact tccaagatga atgcccaaca gctccgccat gcagtccggc agtggctgaa
     7381 ggtcgaacgt accgggctgc tggtgatttt ggagtccttc gggttcaaat acggtgtgcc
     7441 gaacaacgcg gattttatgt tcgatatgcg cagtctgccc aacccgtatt acgatcccga
     7501 gttgaggcct tacaccggta tggacaagcc cgtttgggat tatttggacg gacagccgct
     7561 tgtgcaggaa atggttgacg acatcgaaag gtttgttacg cattggttac cgcgtttgga
     7621 ggatgaaagc aggagctacg ttaccgtcgc catcggttgc acgggaggac agcaccgttc
     7681 ggtctatatt gtcgaaaaac tcgcccgaag gttgaaaggg cgttatgaat tgctgatacg
     7741 gcacagacag gcgcaaaacc tgccgagccg ctaatcccgt tgaacgatga tgccgtctga
     7801 aacccgattt tctgtttcag acggcatttc aaatattgag aagaaaggaa agacatggca
     7861 atccaatggt ttcccggcca tatgaacaaa gcgaaaaaag ccatcgccga gcgcgccaaa
     7921 agcgtagaca tggtgattga aatgctggat gcgcgaatgc ccgcctccag cgaaaacccc
     7981 ctgcttgccc agctttccaa aggcaaaccc aaacttaaaa tcctaaacaa acaagatctt
     8041 gccgaccccg aacgcacaaa ggtctggctt gaccactaca acagccgccc cgacacgtgc
     8101 gccattgccc tcgattcctc cgaaaccggc gcacacggca aaatcaccca agcctgccgc
     8161 gcgatgattc cccatcggca aggcatagac aaacccttgc gcgtcttgat ttgcggcatt
     8221 cccaacgtcg gcaaatccac cctcatcaac ggcatgatag gcaaaaaatc cgccaaaacc
     8281 ggcaacgaac ccggcatcac caaagccgaa caacgccttt tccttgccga cgacttctgg
     8341 ctgtacgaca cccccggaat gctgtggccg aaaatcattg tcgaagaaag cggctacaac
     8401 ctcgccgccg gcggcgcagt cggacgcaac gcattggacg aagaagaagt cgccctcgaa
     8461 cttttagact acctccgccg ccactacctc cctatgttgc aagaacgcta ccaagccgac
     8521 aaagacccca gcagccactg ggacgaaaac gtttggctcg aatggatagc caaaaaacgc
     8581 ggcgcagtcc tcagcggcgg acggatcaac taccaaaaag ccgccgaaaa catcctcacc
     8641 gacttccgtg aaggcaaaat cggcagaatc accctcgaaa cgccgaacca atgggaaact
     8701 tggctcaaaa aagcccgtca gaaagaagcc gaactcaaag ccatacgcga agccagaaaa
     8761 gcagagcgta aagggaagaa gccttcggaa gcataaagaa tgccgtctga aaaatatttt
     8821 tcaggcagct tctctctact ccaaccgatt tcagacggca tatccaaacc catgccgttt
     8881 cagcacggat acccgtatga ccgacaaaat ttctcccgac gcgctgattg aagccgcact
     8941 gctgacccaa accgaaccgc tgaccgaaaa atctatgcgc gaattgtgtg tgccgccact
     9001 gtcgcaggac aaactgattg atgtgttggc gcagttgaaa acgcgttggc aggatagggc
     9061 gttgcaactg gtgcatacgc aagagggctg gcgttttcag attgttcaga cggcattcga
     9121 gcggctgggc agcctgcaag aacagcgtgc gccgcgctac tcccgcgccg tgatggaaac
     9181 actggcgatt atcgcctacc agcagcccgt aacgcgcggc gacatcgaag gcatacgcgg
     9241 tgtggcggtg tcgcagaacg tgatgcagac cttgcaggat cgggggtgga ttgaagtcat
     9301 cggacatcgg gacacattgg gaaaacccgc attgtgggcg acaacggcaa cttttctcag
     9361 cgatttgcgc ttggacggtc tggaagaact tccgccgctg accgaactgg gcgaactggt
     9421 tttacccgat ttaatggaaa tgccgcctac ggatgaagaa gagccggaag ccgtaccgtc
     9481 cgataccctg cccaactgaa attccaaatg ccgtctgaaa cgcacattgc ttcagacggc
     9541 atttcaacaa ataagcagat aaaaacaagc actaagcaaa attaaggaaa aacttatttt
     9601 taatttaaaa aatcttagtt ataattcgta tatctaaagt tgatattgct tttgtcggta
     9661 gaattgctaa ggaatcctca cgatgcttct aacactttct ttgcgtgatt ttgtcattgt
     9721 cgaaaatctg aatctggatt ttcaaagcgg ctttaccgta ttgaccggag aaactggcgc
     9781 gggcaagtcc attactttgg atgcgattgg tctgctgttg ggcgataaag ccgattacag
     9841 ccaagtccgc agcggcgcaa aagaagcgca gttgtcggcg ttgtttgata tttcccattt
     9901 acctgtttta aaagcagaat tgtatgaaca ggggctttta aacgacggag aagaagaact
     9961 cagtatccgc cgcattatcg atgccaaagg caaaagccgc agctttatca acaatcaggc
    10021 cgctaccttg gcgcaactca aagccgtcgg cagccagctt atcgacatcc atgggcaaaa
    10081 cgcccatcat tcgcttaatc aggaagccgc ccagcgcgaa ttgttggacg catttgcggg
    10141 cagcagggcg caggcggaaa ccgtcaggca gctttatcaa aactgggcaa actcaaaaaa
    10201 agcattgcag gaagcgcagg aacaggcaga aaacattgca ctcgaacaag agcgtctgga
    10261 atggcagttt aacgaattga atcagttgga cattaaacaa ggcgaatggg aagccctcag
    10321 ccaaagccac gacagccttg cccattctgc cgagctgttg caggctgccg aagaagtcgg
    10381 aagcaagatt gacggcgaca acggcatcca acgccatatc tatcaatgtc aaaaactatt
    10441 ggccaatctg caaaacatcg agccgcgctt tgccgagagc ctgaatatgt tggcaagcat
    10501 cgaagccgaa ttgggcgaaa tcagtgccaa tatgcgcgat gtggcaggtc gcagcgacat
    10561 caatcccaac gaacttgccg cacaagagca gcgtatgggc gagctgatgg ggatggcgcg
    10621 gaaataccgg atcgagcctg aagagttgcc tgccaagttg gcagaaatcg aagaacgcct
    10681 gcaaagcctg caagctgccg ccgatttgga cgcgctcgag cataatgttg cccacaattt
    10741 tgccgaatat caggaagctg cccacatcct ttctgccatg cgccatcagg cggcagagcg
    10801 tttgagcggc gaaacgaccg agcatatgca acaccttgcc atgaaaggtg cacgtttcga
    10861 catcgtcctg ttgccctcgt cgccgacggc acacggtttg gagcaggttc aatttcaagt
    10921 tgccgccaac aaaggcaatc cgccccgtcc gctgaataaa gttgcctccg gcggcgaatt
    10981 ggcgcgtatc agccttgcct tacaggttgt tgccagccaa tatacccaag ttcccaccct
    11041 gatttttgat gaggtcgata ccggtattgg agggggagtg gctgaaatgg tcggcaaggc
    11101 attacgtgcg ttgggcagaa aacatcaggt gcttgccgtt acccatcttc cgcaagtcgc
    11161 atcctgcggc gaaaaccact ggcgagtgcg caagcacagc gagggagagc aaaccgtcag
    11221 cgaaatcagt atattggatg aaatccaacg ggtcgaagag gttgcccgta tgttgggcgg
    11281 agaagtcatt accgatacga cgcggcaaca tgcggcagaa ttgctgcaac ttgcgtcgaa
    11341 aaatagttta ttttaaaatc aatcagttaa aaaataacta aaaataaaag tctaaaacaa
    11401 tagacagaac tcagataaat ccgtattatt acgctttctt aatcacttga acaagtgatt
    11461 gtgctgcacc cgtagctcag ttggatagag tatctggcta cgaaccagag ggtcgggcgt
    11521 tcgaatcgct ccgggtgcgc cagtaagaaa atacaatacg cgcccatcgt ctagcggtta
    11581 ggacatcgcc ctttcacggc ggtaaccggg gttc
//
LOCUS       DAXHTR010000064        11607 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.064, whole genome shotgun sequence.
ACCESSION   DAXHTR010000064 DAXHTR010000000
VERSION     DAXHTR010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11607)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11607)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11607
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.064"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..338
                     /locus_tag="ACFNML_001162"
     CDS             <1..338
                     /locus_tag="ACFNML_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFC6392553.1"
                     /translation="FFDTEPDRGKIEHCACLAMIHEEISAMPMGYETLIGDMGSALSG
                     GQKQRIVLARALYCEPKILFLDAATSHLDIANEKAVNANLNGLSIIKIMAAHRKETVE
                     SADRKMSLG"
     gene            complement(359..1585)
                     /locus_tag="ACFNML_001163"
     CDS             complement(359..1585)
                     /locus_tag="ACFNML_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HFC6392554.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RYGYIYSGRQGWVLKTGADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            1740..1926
                     /locus_tag="ACFNML_001164"
                     /pseudo
     CDS             1740..1926
                     /locus_tag="ACFNML_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779592.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
     gene            2130..2789
                     /gene="queC"
                     /locus_tag="ACFNML_001165"
     CDS             2130..2789
                     /gene="queC"
                     /locus_tag="ACFNML_001165"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFC6392555.1"
                     /translation="MSNQKALVIFSGGQDSTTCLIQAIQIYGRENVQAITFQYGQRHA
                     VELERASWIAQDLGVKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGCRECPSCILRERGLAEYLESKKA
                     V"
     gene            2817..3335
                     /locus_tag="ACFNML_001166"
     CDS             2817..3335
                     /locus_tag="ACFNML_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HFC6392556.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQVVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            3342..3764
                     /gene="queD"
                     /locus_tag="ACFNML_001167"
     CDS             3342..3764
                     /gene="queD"
                     /locus_tag="ACFNML_001167"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFC6392557.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDGIIKGG
                     AKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNSRECQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGE"
     gene            3873..4229
                     /locus_tag="ACFNML_001168"
     CDS             3873..4229
                     /locus_tag="ACFNML_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392558.1"
                     /translation="MNTTSSMIQEEGIIAESIDVINKFVQFLLKLYDDFGIDGMHDLV
                     DPDLDTLESIVKNLQQEVDKLPISPNDFSLENKKISLAQGLLYAQSMITNVRNKDTEE
                     CSRNRSMLKNNQSSLY"
     gene            4249..4374
                     /locus_tag="ACFNML_001169"
     CDS             4249..4374
                     /locus_tag="ACFNML_001169"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392559.1"
                     /translation="MKSMDIRFARIAALTKQLAAIQRQIEKRAYKAKAATMKKVA"
     gene            4479..4850
                     /locus_tag="ACFNML_001170"
     CDS             4479..4850
                     /locus_tag="ACFNML_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HFC6392560.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAETFKLPYEFME
                     QNRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGVTVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            4847..5485
                     /locus_tag="ACFNML_001171"
     CDS             4847..5485
                     /locus_tag="ACFNML_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFC6392561.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIYDTIRQIGILNSRPDASVHWQLSVQTHKWAGIE"
     gene            complement(5820..5896)
                     /locus_tag="ACFNML_001172"
     tRNA            complement(5820..5896)
                     /locus_tag="ACFNML_001172"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5860..5862),aa:Pro,seq:cgg)
     gene            complement(5977..7062)
                     /gene="nagZ"
                     /locus_tag="ACFNML_001173"
     CDS             complement(5977..7062)
                     /gene="nagZ"
                     /locus_tag="ACFNML_001173"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFC6392562.1"
                     /translation="MTVPHIPRGPVMADIAAFRLTGEEKQRLLDPAVGGIILFRRNFQ
                     NIEQLKTLTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHSNPEAVTRLALAL
                     QKGLAKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLDELEAADLIPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELRDDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTAD
                     FQAAQAFVAALASPQDTAGGVKVGEAF"
     gene            complement(7119..8639)
                     /locus_tag="ACFNML_001174"
     CDS             complement(7119..8639)
                     /locus_tag="ACFNML_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HFC6392563.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETTVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            complement(8841..10340)
                     /locus_tag="ACFNML_001175"
     CDS             complement(8841..10340)
                     /locus_tag="ACFNML_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HFC6392564.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIKHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSSNAENDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLRAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            complement(10478..11107)
                     /gene="nth"
                     /locus_tag="ACFNML_001176"
     CDS             complement(10478..11107)
                     /gene="nth"
                     /locus_tag="ACFNML_001176"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFC6392565.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            complement(11153..11566)
                     /locus_tag="ACFNML_001177"
     CDS             complement(11153..11566)
                     /locus_tag="ACFNML_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFC6392566.1"
                     /translation="MMPAYAYAALAFSAFTSATLLPGTSEAAFALFVHRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLAFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
BASE COUNT         2792 a         3036 c         3194 g         2585 t
ORIGIN      
        1 gttttttcga tacggaaccg gatagaggaa aaatcgaaca ttgcgcctgc cttgctatga
       61 ttcacgaaga aatctccgcc atgcctatgg gctatgaaac cttgatcggc gatatgggca
      121 gcgcactgtc aggcggacaa aaacaacgca tcgtattggc gcgggcctta tattgcgaac
      181 cgaaaatcct atttttagat gcagcgacca gccatttgga tattgccaat gaaaaagcag
      241 tcaatgcaaa cttgaatggc ttgtctatca taaaaattat ggcggcacac agaaaggaaa
      301 cggtggaatc agcagatagg aaaatgtctt taggataaaa atacagtttc aaaaatactc
      361 aagattgctg ctgttttttc gcctgagcgt caaactctgc cagcgtcatg ttcaaagtct
      421 gcaaacacgg catcattact gcatcgacag cttggttcac atgatccctt tccacaggca
      481 acggacggta aacgaagagc ttgaagagtt cgttcaactc aatcgaatcc gcccccgttt
      541 tcaacaccca accctgtctg ccggaataga tatagccgta ccgcgccagc ttttccaaaa
      601 gctcgcccaa ctcgtcgtag cccatattga tatgccgtct gaactcctga acaggcaagg
      661 ctttgccttc tttttgcgcc gcatccagaa gcagcaggat tttcaacacg tcgtcaaacc
      721 gtccgcgcga atcgaagccc ctgcggaacg cttctccctg ccagtaggag agtgaagaag
      781 tcagcaccgc gccgcccaag accagcgtcc acaacaggtt cagccacaac agaaaaaacg
      841 gcacggcggc aaacgcgccg taaatcgagc ggtagccgtc gaaattgccc atataccaag
      901 tgaagaggga gcgcgcggtt tccagacaaa acgctgttgc caaagccccg acaaacgcct
      961 gccgcgcggg aacgaagcgg tttggcacga agcggtacag cccccacagc aaaagcgtca
     1021 tgaaggtcag cgtcgccgcc gttcgcaacg cgcccgacca ctgcggcgca cctgaggcaa
     1081 gcgcggcatc ctgtaccgag ccgaccataa aggaaatgcc cacgcccaaa gacagcggcc
     1141 cgaacgtcag taaagcccaa tagacgagaa actgcatcat ccacggacgc tgggaattga
     1201 cccgccagat gcggttgaac gtattgtcta tcgtccgaat cagcatcagc gaggtaacga
     1261 ccagcatcac gctgccgatt gccgtcagcc ggttcgcctg ctcgcggaac gcattgatat
     1321 agtcgaacac catgtccgcg ccctgcggca caatggtttg gttgacgaag gagacgaacg
     1381 aatccgacca gcggtcgaac acggggaaaa tcgaagcgac cgccaccatc acggtcagca
     1441 cggggacgag tgccagcagc gtcgtaaacg tcatgcttgc cgccgcctgc ggtacgcgtt
     1501 cttcatcaaa gcggcggacg acgaaccatg caaacgcaca gattttattg tctgccaaac
     1561 cttgcaaacg ttgtaaaaag gtcataattt cttgcccggt cagtaagttg ggcattgatg
     1621 cccgatgtta tagccaattt tgccgtcagg aacaaatgcc tgaactgcgg ctgtttcaga
     1681 cggcatcgga acaactgtta tgccgtctga agaccgaacc attttaacgg aatccgccca
     1741 tgaacccaaa tccccctcaa aatcctcgtc ctctactatt tccaaaacgg cagcacccgc
     1801 aatcccgcac tccgaatcgc tcgcggcatc gacagcgttg aaggttgcga agccgtattg
     1861 cgcaccgtcc ccaaagtctc cgccgtctgc gaagccgtca aaaaagatat tcccgacagc
     1921 ggctcccgtc ctgaccgccg aagaaaacaa tatcgccttc gcacaaagca aacgcttggc
     1981 ggaactcgcc gtcaagtcgg cataagccgc gtgttcagac ggcatggcgt tcagatgccg
     2041 tctgaacgcg tttgcctgta taatccgcgt ctttactgtc caacttcgcg gttcgcaaac
     2101 ctcccgcgtt accaaaacta ggattcgata tgtcaaacca aaaagccttg gtcatctttt
     2161 cgggcggtca ggattcgacc acctgcctga ttcaggcaat ccaaatctac gggcgcgaaa
     2221 acgtccaagc cattactttc caatacgggc aacgccatgc cgtcgagctg gaacgtgcca
     2281 gctggattgc gcaggatttg ggcgtcaaac aaaccgtact cgacttgagc ctgatgcggc
     2341 agattacgca caatgccctg atggacgaca ccgccgccat cgaaactgcc gaaaacggcg
     2401 ttccgaatac ctttgtagac ggccgcaacg cgcttttcct gctctatgct gcgatttacg
     2461 ccaaagggca ggggatacgg cacatcatcg cgggcgtgtg cgaaaccgac ttctccggct
     2521 atcctgactg ccgcgacgtg ttcgtcaaat ccatgaacgt tacccttaat ctggcgatgg
     2581 actatgattt ccaaatccac acgccgctga tgtatctgac caaggcgcaa acttgggcgt
     2641 tggcggacga aatgggcgcg ctggactata tccgcgagca aacccacacc tgctataacg
     2701 gcatcgtcgg cggctgccgc gaatgcccga gctgtatctt gcgcgaacgc gggctggcgg
     2761 aatatctgga aagtaaaaag gccgtctgaa cacgcgcaaa ccataaggaa tacgatatgc
     2821 ccaagctcca tatgttttac ctcggcggca atgccggcag gtcgaatatc gaagtgcacg
     2881 acatccaatt tgccgtgtgc gacgactacc gtgaagccgt ccccgcactc aaagccgcgt
     2941 ggttcggcga tgcggacaaa atccacatcg acggctggca ggttgtcgaa tgggcggacg
     3001 gttacgacat cgccgtatcc gaaacgccca aaacgaaaat gccgtctgaa cacgccccgc
     3061 gcctgtattt cgccaatgtc ggcggttatc gcgcagggca gctcgccgaa gcgcacgctt
     3121 ttgggctgtt cgccgccgcc acgcctgccg aagccaaaca aaaagccctg caaaccctgt
     3181 tgaccgacag ctatgttcag caacataaag acaacttaaa agacgtggac aacctgcttg
     3241 cgctcgaccg catcggcaat ttccatatcc gcctgacccc gaatccgcac ggcaaacccg
     3301 ccgaaatcgg ctttcaaggc tatttgccca tttgagaacc catgaaaatc accaaaatct
     3361 tcaccttcga ctcctcgcat atgctcgacg ggcatgacgg caaatgccaa aacctgcacg
     3421 gacataccta caaactcgaa atcaccgttt cagacggcat tatcaaaggc ggcgcgaaag
     3481 acggtatggt gatggacttt accgacttga aagccattgt caaacaacac attaccgacc
     3541 ccttcgacca cgccttcatc taccacggcg gcaacagccg cgaatgccaa atcgccgcgc
     3601 ttttggaggg ctggaacatg aaaaccctgc gcctgccctg ccgcaccact gccgaaaata
     3661 tggcggtcga aatgtacgac cgtctgaaaa acgcggggct gaacgtgtgc cgcgtcaaat
     3721 tgtgggaaac gccgacatcg tgtgcggagt atgaagggga gtaatattga aacaaatttt
     3781 aaagatttgt tttgacttgt catataatca attgtaaaat tccatacttt ctaaaaaatc
     3841 gaccgtaata gaaaatttca ggcataataa ccatgaatac aactagtagt atgattcaag
     3901 aggaaggcat tattgccgag agcatagatg taattaataa gttcgtacaa tttcttttga
     3961 aactgtacga tgattttggg attgatggta tgcatgattt agttgatcct gatttggata
     4021 ctttggaatc cattgtgaaa aatttacaac aagaggttga taaacttccg atcagtccca
     4081 atgatttttc tttggaaaac aaaaaaatta gtttggctca aggacttctc tacgcacaat
     4141 ctatgattac taacgttaga aataaagata ctgaggaatg ttctcggaat cgttctatgc
     4201 tcaaaaataa tcagtcgagc ctttattaac aacagcaagg agttatttat gaaatcaatg
     4261 gatatccgtt tcgctcgcat tgctgcatta acgaagcaac ttgcagctat tcaaagacaa
     4321 attgaaaaac gagcttataa agcaaaagct gcaaccatga aaaaagtagc atgaaaagtt
     4381 ttggtagaga agccatccat aatgggtggc ttttttgtta aataaaattg gtaaagtttg
     4441 ttaaatataa atcttagtgc aaattaaggt gcttacccat gaaactcctc ttcatcctcc
     4501 tagtcctctt cgtcgccgtc gaacatttct acatcgcctg gcttgaaatg acacagattc
     4561 ccagcgaaaa agcggcggaa acgttcaagc tgccttatga atttatggaa caaaatcgcg
     4621 tgcagaccct gttcggcaac caagggctgt ataacggctt tctcggcatc gggctggtgt
     4681 ggtcgcggtt tgctgcgccg gataacgcgg tgtacggcgt gacggtactg tttctcggct
     4741 tcgtcctgat tgccgccgcg tggggcgcgt tctcttccgg caacaaaggc atactcgtca
     4801 aacaaggttt gcccgcattt ttggcagcgg cggcggtgtt ggcggtatga aaaaaatcaa
     4861 tgttgccccc gaaaatcccc aataccgcat cgtcgaaatc tttgaaagcc tgcaaggcga
     4921 aggctggaac acgggcatgc ccgccgtttt cgtccgcttg ggcaaatgca acctcgcctg
     4981 cggctggtgt gataccgatt atttgacatt cggcatgatg agcctgtccg acatcttagg
     5041 ctgtctgaaa acctatgccg cccgcaacat catcatcacc ggcggcgaac cgaccataca
     5101 gccgcatctc gatatgctgc tggacacgct caaggcggaa ggctatttcc tctgtctcga
     5161 aaccaacgga ctcaatcccg cgccgccgca aatcgactac gtcgccacca gccccaaagc
     5221 ctgctacgcc gccaaatatg aaaatagctg tatcgaaaca gccgacgaag tgcggattgt
     5281 tgccgatggt gatgtccttg cgttctgcga aaacatggaa cgcaaaatcc gcgcacatca
     5341 ttactacctt tcgccctgtg agcaagacgg tgcgatgaac atctacgaca ccatccgcca
     5401 aatcggtatt ttaaacagtc gccccgacgc atccgtgcat tggcagttga gcgtgcagac
     5461 gcacaaatgg gcgggaatag agtagtttaa gcagtgtaac tcaaagggcc ggcgtaccgt
     5521 tttaccgatg tttgacatac ggggaaagtg tgccgcttct gcgtggaaat gccggtattt
     5581 ccaccgccca atcaggacgg agccttactg aataagatgc tgccgttggg tacaagcccg
     5641 gcttcctaaa ttccgatggt cttttgaacc ttgccgatac cctttgtcag tgcacgcaaa
     5701 tagaagggtt agggaaaacg aaatgccgtc tgaaacagaa tgctgtttca gacggcattt
     5761 ttctgttgac gccaaaagga aaaaccgctc cggcaatgga tgccgaggcg gtttggattt
     5821 ggtcggaatg agaggattcg aacctccgac cccttcgtcc cgaacgaagt gcgctaccgg
     5881 gctgcgctac attccgaatt aagtaaggcg tgattatagc gcaaaaaata cggcgtgcct
     5941 ataccgtttt gcctttttgc cgcgtgtcgg gcggatttaa aaggcttcgc cgacttttac
     6001 gccgcccgct gtatcctgcg gcgaggcaag ggcggcaaca aaggcttgcg ccgcttggaa
     6061 atccgccgtc tgcataacgg cttgcgcggc ggcgctgccg agcgtgttgg ccatgtattg
     6121 ccaacgttgc gccaaagcgg gattgtcggg aatgcggaaa tcatcgcgca gttcgtccac
     6181 aaggtcggga cggttgcaga cgaggacaat gtcgcaacct gcctcaaagg aaatgcgggc
     6241 gcgttctttg atgccgcctg ccccgcacgc gccctccata gtcaaatcgt ccgagaaaat
     6301 cacgcctttg aacccgatgt cgcggcgcaa aatctgtttc agccagattt cggaaaaccc
     6361 ggcgggcttt gtgtccactt gtggataaac gacgtgggcg ggcataaccg ccgccatacc
     6421 ttcgcggctc ataatgcgga aaggaatgag gtcggcggct tcgagttcgt cgaggctgcg
     6481 cccgtcttcg ggtagcacca aatggctgtc cccttcgaca aacccgtgtc cgggaaaatg
     6541 tttgccgcag gatttcatac cgccttttgc caaacctttt tgaagggcga gggcgaggcg
     6601 ggttacggct tcggggttac tgtggaaact gcggttgccg atgacggggc agtttcccca
     6661 gtccaaatct aagacgggcg tgaaggacaa gtcgatgccg caggcggaaa gctcggttgc
     6721 caaaacccgg ccgacttgtc cggcggcggt ttcggcggcg gacgcgccgt ctttgtccca
     6781 aatctcgccg agcgtactca ttgcgggcag gcgggtgaag ccttcgatga aacgttgcac
     6841 ccttccgccc tcgtgatcga cggcgatgat gagttcgggt gtgcggaggg ctttgatttc
     6901 ggcggtgagt gttttgagct gttcgatgtt ttggaagttg cggcggaaga ggatgatgcc
     6961 gcctacggcg ggatcgagca ggcgttgttt ttcttctccg gtcaggcgga aggcggcaat
     7021 gtctgccatg acggggccgc gcggaatatg ggggacggtc attgcggttt gctccaaaaa
     7081 gcttcagacg gcatatgccg tctgaacagg gaaaggggtc aggcgttggc gcgtttttta
     7141 tctttcaaca gaaaaatcag caccgccaat acaatgcctg tcgtgccaaa acccaacagc
     7201 gcggattttg tcagacccaa tgcgaggtag cccgatgcgg cggcggcggc aacggttaag
     7261 gcgtaaggca gttgcgaggt aacgtggtcg atgtggttgc agcgcgctcc ggtggacgac
     7321 aggatggtcg tgtcggaaat gggcgagcag tggtcgccgc ataccgcccc cgccattact
     7381 gcggacatac acgggataat cagcgcgggt tcgactttga ccgccatggc ggcggcaatc
     7441 ggcagcataa tgccgaacgt cccccagctt gtgcctgtgg caaacgccat cacgctggcg
     7501 agcaggaaga ggatgacggg caggaagccg ggatggatgt tgcccgcaac cagcgtggag
     7561 aggtaatcgc cggtgtgcat ttcgccgaca accgtactga tgagccaagc gaggattaaa
     7621 atggcgattg cgccgaacat agatttcgca ccctgccaaa cggctttggg atagtcggcg
     7681 gttttaatcg tgccgagcgt gcagagaacg acggcaagga cgccgcaagt gccgccgaat
     7741 accagcgaag tgtttacgtc ggtattttca aatgccccca aaatgctgaa ggtttcgctt
     7801 gcctgcgcgc cggtgtagat catggcggaa accgttgagg cgattaaggc caaaacggga
     7861 ataatcagtg cgtaaacacg acctttggta gcgtctgaaa cggtagtttc atcgtgggct
     7921 tcgttcaacg cggcttgttc gaaacgtgcc atcgagccga tgtcgaagga aaaccatgcg
     7981 acgacgaaca ccataatcag ggcaaacagt gcgtaatagt tcatcaggct catggcgaca
     8041 aacgtcccca tcggcgtgta ttcggtgatt ttgtaggtaa cgagcagtcc ggcaagcgtg
     8101 gcgataatcg acgcgcccca gcttgaaacg ggcatcagca cgcacatagg cgcggcagtg
     8161 gagtcgagga tgtaggcgag tttggcgcgg gaaactttaa acttgtcggt aacggggcgg
     8221 gcaatcgcac cgacggcgag actgtggaaa tagtcgtcga taaaggttac aaacacgagg
     8281 caggcggtca gcattttcgc gccgcgccgg tttttaatgt gccgttttgc ccagtcggca
     8341 aacgcctgat tgctgccgga gtaggtcagc agggaagtaa aaatacccaa aagtatcagg
     8401 aaaaccaaga tttttggttt gcccagcgac caatcgccgt ctgaccaagc caagccgacg
     8461 accatgtctt tcaggtgtgt cagaccgtcg acggggttgc cgccgaccaa aaaggcaacg
     8521 ccgaccagaa taccgatgcc taaagacagc agtacgcggc gggtaatgac ggcaagtgcc
     8581 agtgccaaaa agggtggcac aaccgagaaa aatgaatgtg aatagtcgat cagctgcatg
     8641 gttatggggg tattaagcgt ccggatggga gcgtatctgt ccgcctccgg tttgggtttt
     8701 gttggcaaaa tgggcggaaa tattttttgt cgtaaaaaat atttgtttaa aatcaaccaa
     8761 ctgatttttg taaaatgccc gttaatcggt attgacgggc attttatcat ttaaaaaata
     8821 ttttggttaa attatgtggg ttattgcagg tttaatgcga taaacagcgt gttgccacgg
     8881 cgcatgacca gcagggggac gtttttgcct gccttgtcca tagctttgcg gaaaccggct
     8941 tcgtcattga cggggacttg cccgacggca agaatttcgt cgccgcgcct caagcctgcg
     9001 cgttctgccg cgtcggaaac ccgtacgacg acgaggtgtc cgccgctgct gtcggtatgt
     9061 gtctgaaggg taatgcctgc ggattcgacc gagaacgtac cggattgctg ttcggtgtag
     9121 ggggcttcat ctgttttgga tgatgcgccg atatgctcgg cggcgttgcc cagcttgact
     9181 ttgattgtga tttcttcgcc tttgcgccat acgccgaggc tgacttcttt tcccggcgta
     9241 atggcgccga ccataacggg aaggtcgccg gaagaacgta tttctccgcc gtcgaggctg
     9301 aggacgatgt cgcccgcccg caggccggca cgttctgcgg ggctgccggg caggattttg
     9361 gcaatcagtg cgccgccggc tttgtccaaa ccgaacgatt gtgccaaacc gtaggatact
     9421 tcttgaataa tcacgcccag ttgtccgcgt tggactttgc cggtgttttt cagctgttcg
     9481 gcgacattca tggcaacgtc aatcgggatg gcgaaggaaa tgcccatgaa tccgccgctg
     9541 cggctgtata tttgcgagtt gatgccgacg acctgtcctt ttaagttgaa cagcgggccg
     9601 ccggagttgc ccggattgat ggcaacgtcg gtttggatga agggtgtgta gctttcgttg
     9661 ggcaggcttc tgcctttggc ggacacgatg ccggcggtca cgctgttgtc gaagccgaag
     9721 ggcgcgccga tggcggcgac ccattcgccc ggtttcaaat ctttgggatt gccgattttg
     9781 acgacgggca gctcttccgt tgcgtcgatt ttcagaaggg cgacatcgga ttggacatcc
     9841 gaaccgatga gtttggcggt atattcgcgc ttgtcgttga gcaggacttt gatactgccc
     9901 atgccggtaa cgacgtgcgt attggtcaga atatagccgt ctttgctgat gatgaagccc
     9961 gaaccgaagt tcaatccgcc gtcatctgct tcttcttggg ggatttcggg catgttcggg
    10021 acgaggcgtt tgaaaaattc gtagaacggg tcgctgtcgg caagcgggtc ggaatcgttt
    10081 tcggcattgc tgctgccgtt ttgggtgcgc ggggcggggg ctgcctgaat attgacgact
    10141 gccgggcctt cgctttgaac cagttggaca aagtcgggca gcagcatact gacgctgccg
    10201 tcgtctttgg tgtgtttgat gcgttctacg aaggatgctt cttttttgtc cgcaccgaaa
    10261 aagctgccgg ctttgtcgca gcctgccagc gaggcggcac acagtgctgc caaagcgagg
    10321 tattggtatt ttttgaacac gttttgtcct ttgtcggatg ccggtaccgg ctttaatgcc
    10381 gtctgaagcg cattttgtcg gcttcagacg gcataggttg aaattctaca acgtccgtcc
    10441 gaattttcaa gcgtttcatt ttgaagggcg gcggcggtca ggctttggcg ggatattcgc
    10501 acaaatcgtt gatgatgcag gtttggcatt gcggtttgag tgccttgcag gtgtagcgtc
    10561 cgtgcaaaat cagccagtgg tgcgcgtcca tcagaaattc tttaggaatg aagcgcatca
    10621 gtttgtcttc gacttcgcgc acatctttcc cgggggcgat tttggttcgg ttggacacgc
    10681 ggaaaatatg cgtatcgacc gccataacgg ggtgtccgaa cgctgtgttc aataccacgt
    10741 ttgccgtttt gcgccccacg cccggcaacg attccaaagc ctcgcggtct tccggcactt
    10801 cgccattgta tttttccagc aggatgcggc aggtttgcat gatgtgcttg gacttggttt
    10861 tatacagccc gatggttttc gtgtattcca tcacgccgtc caaacccaaa tccagcatcg
    10921 cctgcggcgt atcggcaacc ggaaacagct tcgccgtcgc tttgtttacg ccgacgtcgg
    10981 ttgcctgtgc cgacagtaaa acggcgatta aaagctcgaa aggggagttg aagttgagtt
    11041 ctgtggtcgg atgggggttg gcggcgcgga agcgttcgaa gatttcttgg cggatgtgtc
    11101 tgttcatttt tttatacggt gggtttgtgt gttcggcatt ataacgtatg gttcaggcgg
    11161 cgtaatattg cattccccac agaatgaagg cgtaacgcgc cgttttgccg ataaccagca
    11221 tcagcccgct tgtccacgga ttcaaccgca gccagccggc ggcaagcggc agtgcgtcgc
    11281 cgacgacggg cagccaggca aacgcaagca gccaaatacc gaaacgccgc atcagattca
    11341 gtgttttttc agacggcatt tttcgggagg gcagcaaacg ccccatccaa taggaaacca
    11401 tactgcccaa tccgttggca aggccggcgc acagcaacgc gccgtatgcg tgttcgggaa
    11461 agcggtggac gaacagggca aaggcggctt cggatgtgcc gggcaggagg gtggcggaag
    11521 tgaaggcgga aaaagcgagc gcggcgtagg cgtaggcggg catcattgca aacagtctca
    11581 aacaggcaaa aatcggcgac ggattgt
//
LOCUS       DAXHTR010000065        11537 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.065, whole genome shotgun sequence.
ACCESSION   DAXHTR010000065 DAXHTR010000000
VERSION     DAXHTR010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11537
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.065"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            207..1640
                     /gene="lpdA"
                     /locus_tag="ACFNML_001178"
     CDS             207..1640
                     /gene="lpdA"
                     /locus_tag="ACFNML_001178"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFC6392567.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHEFAEHGITVGDVKFDVAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGAEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELLTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            1735..2022
                     /locus_tag="ACFNML_001179"
     CDS             1735..2022
                     /locus_tag="ACFNML_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392568.1"
                     /translation="MNKEIVGIFFIPMGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAAMYLLARMWLPFSK"
     gene            2076..3287
                     /gene="sucC"
                     /locus_tag="ACFNML_001180"
     CDS             2076..3287
                     /gene="sucC"
                     /locus_tag="ACFNML_001180"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFC6392569.1"
                     /translation="MEANSGHYTNLKEKSMNLHEYQAKELLAGYGLPVQGGILAHNGE
                     EAAAAYDKLGGKFAVVKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTD
                     ANGQPVNSVLVCEDMYPVQTELYLGAVVDRSTRRVTFMASTEGGVEIEKVAAETPEKI
                     FKVTVDPLVGLQPCQAREVAFQLGLKDKQINEFVKLMTGAYKAFVENDFALFEVNPLA
                     VRENGALACVDGKIGIDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIG
                     CMVNGAGLAMATMDIIKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVQGVLINI
                     FGGIVRCDMIAEAIVAAVKEINVDVPVVVRLEGNNAELGAKILNESGLKLTSADGLND
                     AAEKIVAAVNA"
     gene            3298..4188
                     /gene="sucD"
                     /locus_tag="ACFNML_001181"
     CDS             3298..4188
                     /gene="sucD"
                     /locus_tag="ACFNML_001181"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFC6392570.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(4428..4751)
                     /locus_tag="ACFNML_001182"
                     /pseudo
     CDS             complement(4428..4751)
                     /locus_tag="ACFNML_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4723..4866)
                     /locus_tag="ACFNML_001183"
     CDS             complement(4723..4866)
                     /locus_tag="ACFNML_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392571.1"
                     /translation="MARYRRNFIAGGTFFFTVKLADPKSRLLVEHIGFAYGLYGCAKT
                     ISL"
     gene            complement(5152..6354)
                     /locus_tag="ACFNML_001184"
     CDS             complement(5152..6354)
                     /locus_tag="ACFNML_001184"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6392572.1"
                     /translation="MLIPQTSLFDQENNRIISQRKLLEQSLLQGIPLNELIRQNQMSY
                     DDLIYIHHDKKPFEKRKNLVLSDFQKKENHIPIISFFSGAGGLDLGFEAAGFQHSMLI
                     EKIPLFCDTLRLNRPNWSVFNADVSETEEMVELIRNNIGSLSFDGVFIGGPPCQPFSI
                     ASNQRFSKTDKNFKRVGFEHKQNGNLLFNYVELIKIFRPKVFLIENVSGLMDIDNGKQ
                     LASVYCDLQQQGYSLYKPQIINSEYFHVPQQRIRLFIIGYLGDKKFSPLQPTNTPLAC
                     GNTFEIPIINQHNHITREHSAESILRYMELAYGQRDKKGRVDRLDPSKPSKTIIAGGT
                     KGGGRSHLHPYIPRTLSVRESARLQTFPDDYIFTGAVARQFTQVGNAVPPILAAQLAT
                     AIKHSYFE"
     gene            complement(6367..7551)
                     /locus_tag="ACFNML_001185"
     CDS             complement(6367..7551)
                     /locus_tag="ACFNML_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6392573.1"
                     /translation="MIEWRFDQGRLDYFRFDEIKKIAKGLYGINQCPKPRAKDIDIVR
                     LSLTQETNLPFAPNSSQYPVWRNYGRVFKCLLLATEHNGLICTTDLCADLVQNDEISC
                     DDYLLHFATHFSYPSPAFSGYQPSENRVFPVLAIIRALISKYKETGVNFLDMDEIIYK
                     IASNNSITGIASFSFSQLIMNKEAIKYDPRQIRELVKFISQFSFLSWYENKLFLDITD
                     FSELHLLENQFIADTQPQERLDSLEIMRLGKTNNNFLLMNEIVSLSIDDQAFSEGGKI
                     KVTHLITERNSILRKIYFSKTQNAQICRMCGLDTQRSYEWSEHIIEVHHLLPLSSPVR
                     VEDKTTSLKDLVGICPTCHRATHKYYTFWLNLHKKKDFVSYEEAYHVYEEAKSKVKRI
                     YK"
     gene            complement(7593..10442)
                     /gene="uvrA"
                     /locus_tag="ACFNML_001186"
     CDS             complement(7593..10442)
                     /gene="uvrA"
                     /locus_tag="ACFNML_001186"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFC6392574.1"
                     /translation="MCNHHPQHSHDNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHNLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKANIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDSEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSITGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDSGGRIIAQGSPEEVAKVKGSYTGKYLKVVLG"
     gene            10597..11394
                     /locus_tag="ACFNML_001187"
     CDS             10597..11394
                     /locus_tag="ACFNML_001187"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HFC6392575.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVTGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAAAAKIQAAVQDVLKD"
BASE COUNT         2978 a         2620 c         2909 g         3030 t
ORIGIN      
        1 tagatgtagc gtaatgtagt atcgtgctac aataggctca acaaacgatt gagaccgtct
       61 gaaacatttg attcgaatga atcggcagat atggactttc agacggcttt ttcttaaaac
      121 catcaaaacg cagtcattca aaataaaaaa gaaacgaaaa gtatcgtttt tattttgaga
      181 tactgctaaa agcaaaggat gacacgatgt ctcaatatga tgtagtagtg attggtgcag
      241 gcccgggcgg atacgttgcc gccatccgtg ccgcacaact gggtttcaaa actgcctgtg
      301 tcgatgcagg cgttaacaaa gcaggcaatg cccctgcatt gggcggtact tgcttgaacg
      361 taggttgtat cccttctaaa gccctgttgc aatccagcga acatttccac gcagcgcaac
      421 acgagtttgc cgaacacggt atcactgtcg gcgacgtaaa attcgacgtg gccaaaatga
      481 ttgagcgcaa agatgccatc gtaaccaaac tgaccggcgg cgtgaaattc ctgttccaga
      541 aaaacaaagt aaccagcctg ttcggtacgg cttcctttgc cggcaaaaat ggcgatgctt
      601 accaaatcga agtcgataac aaaggtgaga aaaccgttat cgaagccaaa cacgtcatcg
      661 tagcgaccgg ttccgtaccg cgtccgctgc cacaagtcgc tatcgacaat gtgaacgtat
      721 tggacaacga aggcgcattg aacctgaccg aagtacctgc caaactcggc gtgatcggtt
      781 ccggtgtgat tggtttggaa atgggttccg tatggaaccg cgtgggtgcg gaagttacca
      841 ttcttgaagc cgcgccgacc ttcctggctg ccgccgacca acaaatcgcc aaagaagcct
      901 tcaaatactt caccaaagag caaggtctga gcatcgaatt gggcgtgaaa atcggcgaca
      961 tcaagtctga aggcaaaggt gtttccgttg cttacgaaac tgctgccggc gaagccaaaa
     1021 ccgaagtatt cgacaaactg atcgttgcca tcggccgtat tccaaacacc aaaggcctga
     1081 acgccgaagc cgtaggcttg gaaaaagacg agcgcggctt tatcaaagta gatggcgaat
     1141 gccgtaccaa cctgcctaac gtatgggcaa tcggcgacgt ggttcgcggc ccgatgttgg
     1201 cacacaaagc cagcgacgaa ggcgttgccg ttgccgaacg cattgccggt caaaaaccgc
     1261 atatcgactt caacaacgta ccgttcgtga tttacaccga tcctgaaatc gcttgggtgg
     1321 gtaaaaccga agagcagctc aaagccgaag gcgtggagta caaaaaaggt acttcaggtt
     1381 ttggtgcgaa tggtcgcgca ttggcaatgg gcaaagccaa aggtacggtt aaagtgttgg
     1441 cagacgccaa aaccgaccgc atcttgggcg tacacatgat tggtccggtt gtcagcgaat
     1501 tgcttaccga aggcgtgact gcgctcgaat tcttcgccag cagcgaagac atcgcccgca
     1561 ttatccatgc ccacccaacc ttgtccgaag tggttcacga agctgcattg gcggccgaca
     1621 aacgcgcttt gcacggttga tagacattaa ggcctccgac aaattgaatg ttccgagagc
     1681 ttcgttttct gatttataat tccgtcagac aaacaaacag catttacatt cgttatgaac
     1741 aaagaaatag tcggtatttt ctttataccg atgggcatca tcagcatgtg tatggccgca
     1801 ttgtggcaga tgtatgtgat gatgaccgaa acttatacgc tcaaccgttt caaagataaa
     1861 gaattggttt ggcgcgtggc attgttgttt atcagtttca gccttgccgt ttatctgctc
     1921 tgtccgaatt cgcgtaaaaa aggcattgtc ttttttattc tcgggggagg cggtgcagcc
     1981 atgtatctgc tggcgcggat gtggttgcct ttcagcaagt gaaacgacga ttttccgacc
     2041 gccgaaaggt agtctgaaac gcacgggctt gccatttgga ggcaaactcg gggcattaca
     2101 ctaatctaaa ggagaaatcc atgaatttac acgagtatca ggctaaagaa ctgctggctg
     2161 gctacggttt gcccgtacaa ggcggtattt tggcacacaa cggcgaagaa gccgctgcag
     2221 cttacgacaa attgggcggc aaattcgctg ttgtcaaagc acaagtacac gccggcggcc
     2281 gcggtaaagc gggcggcgta aaagtcgtta aaagccgcga agaagctaaa gaagtggctg
     2341 aaagcctgat tggcaccaac ttggtaactt accaaaccga tgccaacggt caacctgtca
     2401 acagcgtttt ggtttgcgaa gatatgtatc cggttcaaac cgagctgtac ttgggcgcag
     2461 tggttgaccg ttctacccgc cgcgttacat tcatggcttc taccgaaggc ggtgtggaaa
     2521 tcgaaaaagt tgctgccgaa actccagaaa aaatcttcaa agtaaccgtt gatccgctgg
     2581 tcggcctgca accttgccaa gctcgcgaag ttgccttcca actgggcttg aaagacaaac
     2641 aaatcaacga gttcgtcaaa ctgatgaccg gcgcgtacaa agcgtttgtc gaaaacgact
     2701 tcgccctgtt tgaagtcaac ccgctggcag ttcgcgaaaa cggcgcactg gcttgcgtgg
     2761 acggcaaaat cggcatcgac agcaacgcgc tctaccgcct gccgaaaatc gccgaattgc
     2821 gcgacaaatc tcaagaaaac gaacgcgagc tgaaagcttc cgaattcgac ctgaactatg
     2881 ttgccctgga aggtaatatc ggctgtatgg ttaacggtgc cggtttggcg atggccacca
     2941 tggacatcat caaactgaaa ggcggccaac ctgccaactt cttggacgtt ggcggcggcg
     3001 caaccaaaga ccgcgtggtt gaagcgttca aactgattct ggaagacaaa tccgttcaag
     3061 gcgtattgat caacatcttc ggcggtatcg tacgttgcga catgattgcg gaagctatcg
     3121 tggcagccgt taaagaaatc aacgtcgacg ttcctgtcgt tgttcgtttg gaaggcaaca
     3181 acgccgaact cggcgcgaaa atcctgaacg aatcaggcct gaaactgact tctgcagacg
     3241 gcctgaatga cgcagccgaa aaaattgttg cagccgtaaa cgcctaagga gaaaagaatg
     3301 agcgtattga ttaataaaga caccaaagta ttggttcaag gtttcaccgg taaaaacggt
     3361 actttccact ccgaacaagc tctggcttac ggcactaaag ttgtcggcgg cgttaccccg
     3421 ggcaaaggcg gtcaaaccca cctgaacctg cctgtattca acaccatgaa agaagctgtt
     3481 aaagaaaccg gcgcggatgc atccgtaatt tacgttcccg ctccgtttgt gttggattct
     3541 atcgttgaag ccgttgattc aggcgtaggc ttggtcgttg tgattaccga aggcgtacct
     3601 actttggaca tgctcaaagc caaacgttat ttggaaacca acggcaacgg tacccgtttg
     3661 gtcggcccta actgcccggg cgtgattact ccgggcgagt gcaaaatcgg tattatgccg
     3721 ggccacatcc acactccggg ccgcatcggt attatttccc gttccggtac attgacttac
     3781 gaagccgtgg cacaaaccac caaactgggc ttgggtcaat caacctgtat tggtatcggc
     3841 ggtgacccga ttcctggtat gaaccaaatc gacgcactga aacttttcca agaagaccca
     3901 gataccgacg ccatcatcat gatcggtgaa atcggcggta ctgcggaaga agaagcagcc
     3961 gaatacatcc aatccaacgt aaccaaacct gttgtaggtt acattgctgg tgttaccgct
     4021 cctaaaggca aacgtatggg ccacgccggt gcgattattt ccggcggcaa aggtactgcc
     4081 gaggaaaaat tcgccgcttt cgaaaaagcc ggtatcgctt acacccgcag ccctgccgag
     4141 ttgggcacga cgatgctgga agtgttgaag gcaaaaggtt tggcataatc aggtttgaca
     4201 actgattaaa catcaaatgc cgtctgaaac cggaaatcgg gtttcagacg gcattttgtt
     4261 tgtcatttca aaaagaggca gcctcaacat acccacatta ttttttgccc tttgaggcag
     4321 tcagaatatt tatcggtcaa acaggagcaa acccgtaagt caggttgcac atattttttt
     4381 gaagtcaggg aatgataagt atcgcatttg aaaagactga tattattttt gcaggattct
     4441 gttttagcac gcgacattct ttgtttgata ggattagaat ggatatagtc cgcgtaacac
     4501 tgcaaatccg tttcatcgcg cacggtgtgt tcgtaaaaac gccgttgcca aataccgcgt
     4561 tcgttccgcc gctgtttgcc ggcggataag tttttagtat aaggggaata tgcggagaat
     4621 ttggttttaa tcagccgccg gagtagggaa taatccgcat cgtcgggcgg cagcgtccaa
     4681 atggcgtgga tatggttcgg cagcacgcat acggcgacgg tttcaaaggg atattgtttt
     4741 tgcacatcca tataagccgt acgcgaagcc gatatgttcg acaagcaggc gcgatttcgg
     4801 gtcggcgagt ttgacggtga aaaagaatgt gccgccggca atgaagtttc tgcgataacg
     4861 cgccatagga tttttgtttg tggtttgggt gttggagaag gaatggtttg ttgtttaagg
     4921 ttttaacttt gttggagctg atgttttcag acgacattgg aggccgtctg aaagcgctgg
     4981 cttcaacgaa gttaaaagtt ggaacggcaa accgttaatc aaccaaccgc gtacgtaccg
     5041 cacacacact gcacgttggc tttgaagttt cgtagactca caggtaggtg tggcgcaacc
     5101 acgcatacgg tgggttgggt ataaaggcta caacttgcta tagcttgctt gctattcaaa
     5161 ataagaatgc ttaattgctg ttgctaattg agcagcaagg atgggaggaa cggcattacc
     5221 aacctgcgtg aattgccgcg caaccgctcc tgtaaaaata taatcatcag gaaacgtttg
     5281 taatcgtgca ctttcacgaa cagataacgt tcttgggata tatggatgta agtgagacct
     5341 tcctccacct ttagtgccac cagcaataat agttttagat ggtttgcttg gatctaacct
     5401 atctacacgt ccctttttat cacgttgccc ataagctaat tccatatagc gtaaaatcga
     5461 ttcagccgaa tgttctcttg taatatggtt atgttggtta ataattggaa tttcaaatgt
     5521 attaccacat gccaatgggg tattggttgg ttgtagcgga ctaaattttt tatctcctaa
     5581 ataaccaatt ataaatagcc taattctctg ttgaggtaca tgaaaatatt cagaattaat
     5641 tatttgaggt ttatagagtg agtagccttg ctgctgtaaa tcacaatata cactagctaa
     5701 ctgtttacca ttatctatat ccatcaatcc agagacattt tcaataagaa atactttagg
     5761 acgaaaaatc ttaatcaatt caacgtaatt aaataataga ttaccatttt gcttatgctc
     5821 aaagccgaca cgtttaaaat ttttatcagt ttttgagaat ctctggtttg aggctatgga
     5881 gaaaggctga cagggaggac ctccaataaa tacaccatca aaacttaaag acccaatatt
     5941 atttctgatt agttcaacca tttcttctgt ttcagaaaca tcagcattaa acacagacca
     6001 attagggcga ttaagacgca atgtatcaca aaataaaggt attttttcaa tcagcataga
     6061 atgttgaaag cctgctgctt caaaccctaa gtctaatcca ccggctcctg aaaaaaaact
     6121 aattatcgga atgtgatttt cctttttttg aaaatcactt agaaccaaat tctttctctt
     6181 ttcaaaaggc tttttatcat gatgaatata aattaaatca tcatagctca tttgattttg
     6241 gcgtattagt tcattcaatg gaataccttg taataaactt tgttctaata attttctttg
     6301 tgaaataatt ctattatttt cttggtcaaa taaagaggtt tgcggtatta acatagatac
     6361 tcctatctat ttataaattc ttttaacttt acttttagct tcttcatata catgatatgc
     6421 ttcttcataa ctaacaaaat cttttttctt gtgcaaatta agccaaaaag tatagtactt
     6481 gtgtgtggca cgatgacagg ttggacagat accaaccaaa tcttttaatg aagtagtttt
     6541 atcttctacc cttacaggag aagatagtgg taatagatgg tgtacttcaa tgatatgttc
     6601 gctccattca tagctccttt gcgtatctaa tccacacatg cgacatattt gtgcattttg
     6661 ggtcttgcta aaatagattt ttcttaaaat actattacgt tctgtaatta aatgggtgac
     6721 tttaatttta ccaccctcac taaacgcctg atcatcaata gataatgaaa cgatttcatt
     6781 catcaataag aaattattat ttgtcttacc caatctcatt atctctaaac tatctaatct
     6841 ttcttgtggt tgtgtatctg caataaattg gttttctaat aaatgtagtt cactaaaatc
     6901 tgtaatatct aaaaacaact tattttcata ccaagacaaa aaagagaatt gactaataaa
     6961 ctttaccaac tctctaattt gacgaggatc atatttaatt gcttctttat tcataattaa
     7021 ctgtgagaat gaaaaagaag caatacctgt aatactatta ttactagcga ttttgtaaat
     7081 aatttcatcc atgtccaaaa aattaactcc tgtttcctta tattttgaaa ttaaagctct
     7141 aataattgcc aaaacaggga aaacccgatt ttcagatggt tgatatcctg aaaaagctgg
     7201 tgatgggtag gaaaaatgtg ttgcaaaatg tagtaaataa tcatcgcaac taatttcatc
     7261 attctgaact aaatcggcac ataaatctgt agtacagatt aacccattat gctccgttgc
     7321 gagtaacaaa catttaaaaa cacgaccata attacgccaa actggatatt gtgaactgtt
     7381 aggggcaaaa ggtaagtttg tctcttgagt caggcttaat cttacaatat caatatcttt
     7441 tgcacgtggc tttggacatt gattaattcc atataatcct tttgcaattt ttttaatttc
     7501 atcaaatcta aaataatcta atctaccttg atcaaaacgc cattcaatca tttttaacct
     7561 ctatgaaata tcatttttca gtaatctgaa aattatccta aaaccacctt caaatacttc
     7621 ccagtataac tccccttaac cttcgccact tcctcagggc taccttgggc aataatcctc
     7681 cccccgctat cgccaccttc cggtcctaaa tctacaatcc aatccgcagt tttaatcaca
     7741 tccagattat gctcaataat cacaatcgag ttgcctttgc ctttcagacg gcctatgact
     7801 tccagcagca gggcgatgtc ggcgaagtgc aggccggtgg tgggttcgtc gaggatgtag
     7861 agcgttctgc cggtgtcgcg tttggagagt tccaaggcga gtttgacgcg ctgggcttcg
     7921 ccgccggaga gggtggtggc ggactggccg aggcggatgt agccgagacc tacgtccatc
     7981 agggtttgca gtttgcgcga tacggtgggg acggcgtcga aaaattcgcg ggcttcttcg
     8041 acggtcatgt cgaggacttg gctgatgttt ttgcctttgt attggatttc gagggtttcg
     8101 cggttgtagc gtttgccgtg gcagacttcg caggggacgt acacgtcggg caggaagtgc
     8161 atttcgactt taatcacgcc gtcgccttgg caggcttcgc agcggccgcc tttgacattg
     8221 aaggagaatc tgccgacgtt gtagccgcgt tcgcgcgaga gggggacgcc ggcgaagagt
     8281 tcgcggatgg gggtgaacag gccggtgtag gtggcggggt tggagcgcgg ggtgcggccg
     8341 atgggggatt ggtcgacgtt gatgactttg tcgaggtgtt cgaggccgcg gatgtcgtcg
     8401 tatggggcgg gttcttcttg ggcgcggttg agttcgcggg cggtgatttt ggcgagggtg
     8461 tcgttaatca gggtggattt gccgctgccg gatacgccgg tgatgcaggt aatcaaaccg
     8521 agcggcagtt cgagggtaac gtttttgagg ttgttgccgc gcgcgccttt gaggacgagc
     8581 atccggtcgg gattgacggg tgtgcgttca gacggcacgg caatggattt tttgccgctg
     8641 aggtattgtc cggtaatgga gttttcgcat ttggcgacgt tttcgggtgt gtcggcaatc
     8701 agtacgttgc cgccgtgttc gccggcgccc gggcccatat cgacgacgaa atcggcttcg
     8761 cggatggcat cttcgtcgtg ttcgaccaca atcacgctgt tgcctaaatc gcgcaggcgt
     8821 ttgagggtgg cgagcaggcg gtcgttgtcg cgctggtgca ggccgatgga gggttcgtcc
     8881 aaaacgtaca ttacgccggt caggccgctg ccgatttggc tggcgaggcg gatgcgctgg
     8941 gcttcgccgc cggaaagggt ttcggcggaa cgggagagat tcaggtaatc cagcccgaca
     9001 ttaatcaaaa agccgagacg ttcggtgatt tctttgagga ttttttcggc gatttgtttt
     9061 ttgttgccgt ctaaatccag tgtttcaaag aattggtggg ttttggtgag cggccaggcg
     9121 gaaacttcgt gcaacggctc accgctgacg taaacgtagc gggcttcttt gcgcaaacgt
     9181 gcgccgccgc agctcgggca ggcgcggtgg ttttggtatt cgcgcagttt ttcgcgcacg
     9241 gtttcgctgt cggtttcgcg gtagcggcgt tcgagattgg ggatgatgcc ttcgaaggcg
     9301 tggctgcggt tgaaggtggt gccgcgttcg gacaggtaag tgaaatcaat gacttccttg
     9361 cccgagccgt gcaacacgac ttttttgatt tttgcaggta gggtttccca agcagcctgc
     9421 acgtcgaaac cgtaatgccg cgccagcgac tgaatcattt ggaaatagaa ctggttgcgc
     9481 ttgtcccagc cgtcaatcgc gcccgaagcc agcgacaatt cgggatgcgc gaccactttt
     9541 tcggagtcga agaaattggt gttgcccaag ccgtcgcaag tcgggcagga gcccatcggg
     9601 ttgttgaacg aaaagaggcg cggctccaat tcgggcaggc tgtacgaaca tacggggcag
     9661 gcgaaacgcg cggaaaacca atgctcttcg ccgctgtcca tttccatcgc cagcgcgcgc
     9721 tcgttgccgt ggcgcagcgc ggtttcaaaa ctttccgcca gccgctgctt gatgttcgcc
     9781 ttcactttca cgcggtcgat gaccacgtcg atattgtgtt tgatgttttt ttccagcttc
     9841 ggcacttcgt caagctgata gacctcgccg tccacgcgca cacgggcaaa gccttgcgcc
     9901 tgcaagtcag caaagaagtc gacaaactcg cccttacgct cacgaactgc cggcgcaaga
     9961 atcatcacgc gcgtgtcttc cggcagtttt aatacggcat cgaccatctg agatacggtt
    10021 tggctggata gcggcagatt gtgttcgggg caatacggcg tgccgacgcg tgcgtacaaa
    10081 aggcgcaggt aatcgtggat ttccgtaacc gtgccgacgg tggaacgcgg attgtggctg
    10141 gtggatttct gctcgatgga aatcgcgggc gacagaccct cgatcaaatc gacatcgggt
    10201 ttgtccatca tctgcaaaaa ctgccgcgca taggcggaca ggctctcgac ataacgccgc
    10261 tgtccttcgg catacagcgt gtcgaacgcc agcgatgatt tgccgctgcc tgacaatcct
    10321 gttaccacca ccagcttgtg gcgcggaatg tctaaatcga cgtttttcaa attatgcgtg
    10381 cgcgcgccgc gtatgcggat ggtgtcgttg tcgtgcgaat gttggggatg atggttgcac
    10441 ataatggatg ccgcctgaaa aataaaggaa aactggtatt gtagcacttt ctcggatgcc
    10501 gtctgaagcc gcgttcagac ggcatttgtc ggcggagtac ggcagattcc gctataatgt
    10561 cggcaatttt aacccgcttg aacaaaagga tgacaaatga accgtcttta cccccacccg
    10621 attatcgccc gtgagggctg gccgattatt ggcggcggtt tggctttgag cctgctggtg
    10681 tcgatgtgtt gcggctggtg gtctttgccg ttttgggtgt ttaccgtatt tgccctgcag
    10741 tttttccgcg accctgcgcg tgagattccg caaaatcctg aagcagtgtt gagtccggtt
    10801 gacggccgta tcgtggtggt cgagcgcgca cgcgatccgt atcgtgatgt cgatgctttg
    10861 aaaatcagta tttttatgaa cgtgttcaac gtgcattcgc aaaaatcgcc tgccgattgt
    10921 acggtaacga aagtggtcta taacaaaggc aaattcgtga atgcggattt ggacaaagcc
    10981 agcacggaaa acgaacgtaa tgcggtgttg gcgactacgg cttccggtcg tgaaattact
    11041 tttgttcaag tggccggttt ggtggcgcgc cgtattttgt gctacaccca agcaggtgcg
    11101 aaactgtccc gcggcgaacg ttatggcttt atccgcttcg gttcgcgcgt ggatatgtat
    11161 ctgcctgtcg atgcgcaggc gcaagtggcg attggcgata aagtaactgg cgttagcact
    11221 gtattggcgc gtttgccgct gactgcgccg caaatcgaat ccgagcctga gtctgagcct
    11281 gctttacaaa ctgctccggt tgaaacagcg gcaaacccat ctgccgaaca acggcaaatc
    11341 gaggcagcgg cggctaagat tcaggcggct gtgcaagatg tgttgaaaga ttaattttgc
    11401 gaactgaaat agaaaatatc agtaccatca ttcacacgaa tgaggaagtt tggttttttg
    11461 aatttttgct aatgttcaca ccgttatgtt cacgaaaatg ggaatctgga aatttaacgt
    11521 tacggtgatt tatcaga
//
LOCUS       DAXHTR010000066        11251 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.066, whole genome shotgun sequence.
ACCESSION   DAXHTR010000066 DAXHTR010000000
VERSION     DAXHTR010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11251
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.066"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..226)
                     /locus_tag="ACFNML_001188"
     CDS             complement(<1..226)
                     /locus_tag="ACFNML_001188"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6392576.1"
                     /translation="MFDLKNGYTPSKSNKEYWENGSIPWFRMEDIRENGRILDNSLKH
                     ISKSAVKGGKLFPAKSIMMSTTATIGEHALI"
     gene            complement(280..873)
                     /locus_tag="ACFNML_001189"
     CDS             complement(280..873)
                     /locus_tag="ACFNML_001189"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HFC6392577.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTELEAELALRKRQYRYYRDFLLDFDNQIGGG"
     gene            complement(1039..2049)
                     /locus_tag="ACFNML_001190"
     CDS             complement(1039..2049)
                     /locus_tag="ACFNML_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HFC6392578.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTAAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(2046..3590)
                     /locus_tag="ACFNML_001191"
     CDS             complement(2046..3590)
                     /locus_tag="ACFNML_001191"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HFC6392579.1"
                     /translation="MMTEMQQRAQLHRQIWKIADEVRGAVDGWDFKQYVLGTLFYRFI
                     SENFTDYMQAGDSSIDYAAMPDSIITPEIKDDAVKVKGYFIYPGQLFCNIAAEAHQNE
                     ELNTKLKEIFTAIESSASGYPSEQDIKGLFDDFDTTSSRLGSTVADKNKRLAAVLKGV
                     AELDFGSFEDHHIDLFGDAYEYLISNYAANAGKSGGEFFTPQSVSKLIARLAVHGQEK
                     VNKIYDPACGSGSLLLQAKKQFDEHIIEEGFFGQEINHTTYNLARMNMFLHNVNYNKF
                     HIELGDTLTNPKLKDSKPFDAVVSNPPYSINWIGSGDPTLINDDRFAPAGVLAPKSKA
                     DFAFILHALNYLSGRGRAAIVSFPGIFYRGGAEQKIRQYLVEGNYVETVIALAPNLFY
                     GTGIAVNILVLSKHKDNTDIQFIDAGGFFKKETNNNVLTEEHIAEIVKLFADKADVPH
                     IAQNAAQQTVKDNGYNLAVSSYVEPEDTREIIDIKQLNAEISETVAKIERLRREIDEV
                     IAEIEA"
     gene            complement(3663..4667)
                     /gene="rfaD"
                     /locus_tag="ACFNML_001192"
     CDS             complement(3663..4667)
                     /gene="rfaD"
                     /locus_tag="ACFNML_001192"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFC6392580.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMENNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(4708..5827)
                     /locus_tag="ACFNML_001193"
                     /pseudo
     CDS             complement(4708..5827)
                     /locus_tag="ACFNML_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(5829..7064)
                     /locus_tag="ACFNML_001194"
     CDS             complement(5829..7064)
                     /locus_tag="ACFNML_001194"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFC6392581.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDARKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(7101..8072)
                     /gene="hldA"
                     /locus_tag="ACFNML_001195"
     CDS             complement(7101..8072)
                     /gene="hldA"
                     /locus_tag="ACFNML_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HFC6392582.1"
                     /translation="MSAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGNDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNCEVLEQIKQKYREILPEYDAIIFSDYGK
                     GGLSHISDMIDWAKHAGKTVLIDPKGDDYEKYVGATLITPNRAELKEVVGSWKNESEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELIKALSGQSTM"
     gene            complement(8127..8867)
                     /gene="pyrF"
                     /locus_tag="ACFNML_001196"
     CDS             complement(8127..8867)
                     /gene="pyrF"
                     /locus_tag="ACFNML_001196"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFC6392583.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFSNEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRNLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNTAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAASTYL
                     VMGRPVTQAADPVAVLREVNRVANLEAN"
     gene            complement(9393..10040)
                     /gene="adk"
                     /locus_tag="ACFNML_001197"
     CDS             complement(9393..10040)
                     /gene="adk"
                     /locus_tag="ACFNML_001197"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFC6392584.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            10255..11094
                     /gene="htpX"
                     /locus_tag="ACFNML_001198"
     CDS             10255..11094
                     /gene="htpX"
                     /locus_tag="ACFNML_001198"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFC6392585.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FSGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
BASE COUNT         2653 a         2626 c         2671 g         3301 t
ORIGIN      
        1 ttattaacgc atgctctcct atggttgccg ttgtcgacat cattattgat ttagcaggaa
       61 acagcttgcc gcccttaact gcgctttttg atatatgctt caatgaatta tctaaaatac
      121 ggccattttc cctaatatct tccattctaa accaagggat tgagccattt tcccaatatt
      181 ctttatttga tttggaaggg gtatagccat tcttcaagtc aaacacctcc cccaacgtct
      241 tccaaaccac atctttcaga cggcctttat agccatcagc tatccccccc cgatttgatt
      301 gtcaaaatct aaaagaaagt cgcggtaata ccggtattgg cgtttgcgca gggctaattc
      361 agcttccagc tcggtgaatt tgtcaagtat ttttacaatt ttttgttggg tttccagggg
      421 tgggataggg attaataatt ttttcaagtt actggcattt agagcaggaa tacctgtaaa
      481 tgtacataag gcatgaatct cttgttcaaa ttcaagtaat atatgataga gaaaacgaac
      541 atccaattct gttctattct taatagtaac tgcataattt aagttgccac gaaaatatct
      601 tccttcttgc cacgttattg agccgactcc cgctcctctt gttgtgatgc cgatagggtc
      661 attttcagtg ttccattcat caatatagcc aagcggctct ttgccactgt taataactgg
      721 ataactacca atattatttg agattttctg cttacttaca gcttgtcctg tttttatgat
      781 agctatccca ttttctccac ccaacggctt ccactccacc ggtgccgtct gaatcatctc
      841 aatcaatttt ttcgctttgc tttgcatatc catggttgtt ttccgtttct ttcccgctgt
      901 ggcgggatgt ggggcggttg tggggtgctt cggctcactc tgtccgtttg ccgttatttc
      961 tgcgggctga agtccgtcct gctttcaggc tgcctgcaat ggtgtgcagg ctgctttttc
     1021 aggtagcctg aatggtagtt attttgtgcg gtcttttttc tgtttgatct gttgcacggc
     1081 tgttttttcc aacgctttaa cgctttccag ataggcttgc tccaccggcg acaggatgcg
     1141 cgcttcatag gcgcggtatt cgcgttcggc tttctgctct gcctgtttgc ggctgatgct
     1201 gcctgcaccc tgtaatgtgc cttgtccgta cagcccggaa aatttgtcca attcggctat
     1261 ccagtcgcgc atatacatgg ggctttgctc ctgcgctttg atttccgcta ggtcgaagaa
     1321 ggcggaaacc agacggttca ggcggaacag ttcgtcttcg gtcagatagt ttttggcgat
     1381 tttggcttca ttcagcgtgg ggattgcgcc ttgaaaggtg gtcagcccca taaagtcttt
     1441 gctgctgtcg gcacggctgt atatcagctc agctgcggtt tgccggctgg cggcatagtg
     1501 cagtttgttt tgaacggcgg caaaaaaggt ttggctttcg ctgcttttgg ggttgtagtc
     1561 ttggctggtg gcatataaat caagcacttg ccggtatagg gctttttcgc tgctgcggat
     1621 gtcgcgaatg cggttgagca gttctttcca atagtcgccg ccgcctgtgc ctttcaggcg
     1681 ttcgtcgtct atggcaaagc ctttggtcag atattcgtcc agccgttcgg ttgcccattg
     1741 gcggaattgg atgccccgcg cggaacggac gcggtagccg acggcaataa tcatgggcag
     1801 ggaataatgg gcgattttgc ggtttacctt gcgcccgttt tcattttgaa ctgtcaactg
     1861 gaagttgaca gttgccttct cttccaattc ttgctctgca agaatggttt taatgtgttt
     1921 gctgatattt tgtttggtgg tttggtacag ttctgccatg tccgcctgcg tcagccaaag
     1981 ctgtccgcca aattcctgca aggcaaattg ggcagtgccg tcgttggcgg tgtataggat
     2041 gatgctcatg cttcaatctc tgcaatcact tcgtcaattt cacgccgcag ccgttcgatt
     2101 ttggcaacgg tttcgctgat ttcggcgtta agctgtttga tgtcgataat ttcgcgggtg
     2161 tcttcgggtt caacatagct gctgacggcg aggttgtagc cgttgtcttt gacggtttgc
     2221 tgggcggcgt tttgggcgat atgcggcaca tcggctttat cggcgaagag tttgacgatt
     2281 tcggcaatgt gttcttcggt taagacgttg ttgttggttt cttttttaaa gaagccgcct
     2341 gcgtcgatga attggatgtc ggtattgtct ttgtgtttgg acaaaaccag tatattgacg
     2401 gcgatgccgg tgccgtaaaa gagattgggc gcaagggcga tgacggtttc cacgtagttg
     2461 ccctccacca gatattggcg gattttctgc tctgcgccgc cgcgatagaa aatgccgggg
     2521 aatgagacga tggcggcgcg gcctctgccg gaaaggtagt tcagtgcgtg cagaatgaag
     2581 gcaaaatcgg ctttggattt cggggcgagt acgcctgcag gggcaaagcg gtcgtcgttg
     2641 attaaggtgg ggtcgccgct gcctatccag ttgatggaat agggcggatt ggaaacgacg
     2701 gcatcaaagg gtttgctgtc tttgagtttg ggattggtca gtgtgtcgcc caattcgatg
     2761 tggaatttgt tgtaattgac gttgtgcaga aacatattca tgcgggcgag gttgtaggtg
     2821 gtgtggttga tttcctgccc gaagaagcct tcttcgatga tgtgctcgtc aaactgtttt
     2881 ttcgcctgca agagcaggct gcccgagccg caagctgggt cgtagatttt gtttactttc
     2941 tcctgcccgt gcaccgccag ccgcgcaatc agcttggata cgctttgcgg ggtgaaaaat
     3001 tcgccgccgg atttgcctgc gttggcagcg tagttggaaa tcaggtattc gtaggcatcg
     3061 ccgaaaaggt cgatgtggtg gtcttcaaaa ctgccgaaat cgagttccgc cacgcctttt
     3121 aggacggcgg caaggcgttt gttcttgtcg gcaacggtgc tgccgagccg gctgctggtg
     3181 gtgtcgaagt cgtcaaacag gcctttaatg tcttgttcgg acggatagcc ggaggcggag
     3241 ctttcaatcg cggtaaaaat ttctttcagc ttggtgttga gctcttcgtt ttgatgggct
     3301 tcggcggcaa tattgcaaaa aagctggccg gggtagatga aatagccttt gactttgacg
     3361 gcatcgtctt tgatttcggg cgtgatgatg ctgtccggca tagcggcgta atcaatactg
     3421 ctgtcgcctg cctgcatata gtcggtaaag ttttcgctga taaagcggta gaaaagtgtg
     3481 ccgagaacgt attgtttaaa gtcccagccg tccaccgcgc cgcgtacttc gtcggcaatt
     3541 ttccaaattt ggcggtgcag ttgggcgcgt tgttgcattt cggtcatcat cgaaatccat
     3601 ataaaagtta aacaaatcga aatcgcctga tattttcaga cgatttttta cgggcattca
     3661 aattaagcca aattttccag catccatttg acgtagcgct cgacgcctga tttgacatcg
     3721 aaaaattctt ccgtataacc tgcctcacgc aatttggtga tgtcggcttg agtgaagctt
     3781 tggtatttgc ctttgagcgc atcgggaaat gggatgtagc ggataagttc ttcttctacc
     3841 agctctttca agctcatttc gggtttgcct tcggcggcgc ggcaggcgtt gacggtggcg
     3901 gcggcgagtt cgttgaactg ttggctgcgg ccggtgccga ggttgtagat gccggaaagt
     3961 tcgggatggt cgaagaagta gaggttgact ttggcaacgt cttcgacgct gacaaagtcg
     4021 cgggtttgtt cgccgttgcc gtagccgtcg ttgctgccga acaggttgac gtaaccgtgt
     4081 tcgcggtatt ggtggaaatg gtggaaggcg acggatgcca tacggccttt gtgttgttcg
     4141 tgttgtccgt acacgttgaa gtagcggaag ccgacgactt gggcggtcaa gccttctttc
     4201 atgcggcggc gcaatacttg gtcaaacagg aatttggaat agccgtacac attgagcggt
     4261 ttttcgagtt cgcgctcttc acggaagatt tcgcctttac catacacggc ggcactggag
     4321 gcataaagga agggaatgcg ttcgtcctga caccagtcca acaaatccag cgtgtactgg
     4381 tagttgtttt ccatcatata caaaccgtcg tgattcatgg tgtcggaaca cgcgccttga
     4441 tggaaaacgg cttcgatgtt ttgataaggt aaaatgtgtt ccctcacttg gcggatgaat
     4501 tcatgtttgt cgagataatg ggcgatttcg cactcggcaa ggtttttgaa tttttcgcct
     4561 ttgctcaaat tatcgacggc aacaatgtca gtaataccgc gttgattaag tgctttgacg
     4621 atgttgctgc cgataaagcc ggccgcgcct gttacgatga tggtcatatt cggtttcctt
     4681 tgttttgagt aaaattaata aacttagtta caagaaatca tcgatattgt cctgaaacac
     4741 aaatttgcga tgatattcca aatatttttc tcttccttcg ggatatattc tcatcttaat
     4801 tttttgcatt tggattgata ttaagttttt cccatgtgta acggcttagt cgagtaccgc
     4861 aaatcaaacg accatcatcc aagaaagtaa tataaccttg atcgaacaac caatcataag
     4921 tcggcgttaa agctaaccca tttaaataat ctaatgcctc tttctcattt ttctcagtaa
     4981 tacaaaccat ataaggttta atatgactag cccttaacaa aatctcatct gtaattaatg
     5041 taaatggaca ttgcggcata tgatttataa catccttgcg atatttctgc tgcccttttc
     5101 tacttttctg tttaatgatt tcttgctcta cgaattgatt aaattctgaa gccggtattt
     5161 ctaatttttc tcttgataag agttgcgggt gcacgataga gcaaaattga taatccaaaa
     5221 atattcggaa ataaaataat ggttctgaat cttctatatc tttgacaggc aatagtttaa
     5281 gaattgataa ataactaatt ttgggcaata caatcttacg ccaaattgac catatatcat
     5341 cctccgaacg gatataaccc ctacggtaac ctttagaatt ttccaaatgg tctgatacat
     5401 catggattga aaagaaaata ttttctgtag gcagattatt aatacttaaa acgtaatcat
     5461 cataaaacct tgaaatatca tgatgatatt tctgttcttg tttgtgatat tcaacttttg
     5521 catcaaccaa ataacgctgt aaatttttct tattaaaaaa acaatttttc tgaatgaaag
     5581 gatattctac cctccttcca ttttttgtct cagtaatccc ccaattttca tactcaaaaa
     5641 atgtacttag ttttcgacca ctatcatcaa tataactacc tacatatttg cgtgcttccc
     5701 cattaccacc aaaaacagat aacttgttat tcctgtgaat aaaactatct tccgcacgta
     5761 aattttgaat actatcgaca ataaaatact ctatgttttc aatagtaatt ctttgaaaat
     5821 ccggcatttt actctccata aagcgtaata tccatttgtt ttaatatagc gattaataca
     5881 tcaacaacaa tactgtttcc cgcttggcga tacatttgag tatcactaac taaaatttta
     5941 aaatcatctc taaaacccat tagccgcaaa cattccctag gtgttaattt acgaatacgt
     6001 ccacgattat gagtaacata attatcaacc ccggcacgat gcattttatg catagtttgc
     6061 aataatgggc gagctacagg caaatcagtt tccgtactgg ttttaaaatt ttttgttcct
     6121 cctgccaaaa cataattttt gattttttca gaaagataat atttttcctc aacgttattt
     6181 acatcaaaaa taaagtcatc aaattcagat tcgcgggctg cctgaaaaat aaaatcacca
     6241 tgccaattaa attgttgatt tgctttttgg cataaggcaa tttctccatt aatctgtgta
     6301 taacgttttt gtctattttt agaactggta acaaatttca cacccttttc acgtaaaaaa
     6361 tatttgctat cagtatagtc ctccaaaaaa tcttgcatag tatgttctaa ttcaatcttt
     6421 tcaggaaact gaaaaccatt tataggaggg gtatgaaagc caacaacaaa aatacgctca
     6481 cgatgttgag gaatcccata atccttacta ttcattattt ggaaatataa gtcataacca
     6541 agtgaataaa aaacactttt tacaactttc caagtttttc cattatcatg attgagcaag
     6601 ccctttacat tttcataaat aaatatcttt ggttggactt catccacaac gcgagcaaat
     6661 tcatagaata atgttcctcg tgtatcttct aatcctgcac gtttgccaac catggaaaat
     6721 gcttggcaag gactgcctcc aactaaaata tcaacttgat ttctaaactt tctcgcatca
     6781 aattgagtaa tgtcgttatg ccaaaaatct tcatttaatt tatagtttcc aagataactt
     6841 tttttaacgt atggatcaat atctcctgca aaaacaatgg tatggtttaa atttaatcgg
     6901 tggaatgcct gttcaaccgc accaatcccg ctgaatacgg ttgctaatct aatatgtgaa
     6961 tcaggtttaa gaaaagtttt agatttccaa ccttgttgac tgggaaagag caaagttttt
     7021 tgtaatcgag tatcgtgtgt ctgtgccatt gtcgaaatag tcatacttat atcgttctgt
     7081 ttatcttatc aatatgaaaa ctacattgtt gattgccctg acaatgcctt gatcaattcg
     7141 gcaaacgagc aaaccgccgt accgagtttc gccacgacaa ccccggccgc agtattggca
     7201 aggtacatgg cttcgggcat ggtgcagcct gccgccaaac ccaagcccat tccggcaatg
     7261 acggtgtcgc ccgcaccgga tacgtcgtaa acttcttggg cgcgggtggg ctggtaaatc
     7321 ggttcgcctt cgctgaacaa ggtcatgcct tcttcgcttc gggtcagtaa aacggcggtc
     7381 aggtcgaggt ggcggcgcag gttttgcgct ttttcggtca gctcgctttc gtttttccaa
     7441 ctgccgacca cttctttcaa ttcggcgcgg ttaggcgtaa tcagagttgc accgacatat
     7501 ttttcgtaat cgtcgccttt ggggtcgatt aagacggttt tgccggcgtg tttcgcccaa
     7561 tcgatcatat cggagatatg cgacaggccg cctttgccgt agtctgaaaa aatgattgcg
     7621 tcgtattcgg gcaagatttc gcggtatttc tgcttgattt gttccaacac ttcgcagttg
     7681 ggatgttctt caaaatcaag acggataagc tgctggttgc gggcgacgac gcgcagtttg
     7741 acggtggtgg cgatttgttt gtcgcgcatc agataggagg cgacgccgtc ctgcaccatc
     7801 agcgcatcga gcgcgtcggc ggcttcgtcg ttgccggtta cggacaacag ccctgccctg
     7861 ccgcccaacg aagcgatgtt gcgcgcgaca tttgccgctc cgcccgcgcg ttggtcgatt
     7921 cgtccgattt tcgccaccgg cacgggggct tcgggcgaaa tacgggacac atcgccgaac
     7981 caatagcggt cgagcatcac gtcgccgaca accaggactt tggcttgcgc gaaacgggat
     8041 ttgagggttt cttgttggaa cttggcggac atattgcctc cgttgtattt tttcagacgg
     8101 cctcagcctg taaggccgtc tgaaaatcag tttgcttcaa ggtttgccac gcggttcact
     8161 tcgcgcaata cggctaccgg atcggcagct tgggttacag gacgacccat taccaaataa
     8221 gtcgaagcgg cagccaaggc ttcggccggt gtcatgatgc ggcgctggtc atcattattg
     8281 ccggcaacgt ccaagcggat gccgggcgtg accaagacaa aatcttgtcc caactcgcgg
     8341 cgcagcggtg cggcttcttg ggcggaacag accacgccgt ccaagcccga actttgcgcc
     8401 agttttgcca agcggatgac ttgttcttca ggggcggtgt tcaaaccgat ttccgccaaa
     8461 tcgctttgtt ccatgctggt caacacggtt acgccgatta agagcggctt tgtaccgtat
     8521 ccggcaacgg cttctgctgc ggcttccatc atacggcggc cgcccgatgc gtgcatatcg
     8581 accatccaaa cgcccatatc ggcagcgact ttgcaggctt gcgcgacggt gtggggaata
     8641 tcgtggtatt tcaaatcgag gaaaagtttg aaaccttgat tgattaagct ttctgccaaa
     8701 ttgcgtcccg tcgcggtaaa cagctctttg ccgattttga tttgacacaa tgtcgggtca
     8761 aggttgcgga cgaatccgag cgtgtctttt tcgttggaaa aatcaagggc gacgatgacg
     8821 ggggtgcgtt gttgcggagt ttggaagtcg gagattaaag gattcatggt gttttcgttt
     8881 cgggttttca gacgaccttt atcgtctttt tcgcagtata gtggattaac tttaaaccgg
     8941 tacggcgttg tctcgcctta gttcaaagag aacgcttctc taaggtgctg aagcacaaag
     9001 tgaatcggtt ccgtactgtt tgtactgcct gcggcttcgt cgccttgtcc tgatttttgt
     9061 taatccacta taacaacgta aattcaaacc gtaggtcggg cattgatgtc cgacctacgc
     9121 ggactcaagc ctccgacaaa ctgtttcgga taaatttgaa ccggtacatc agccgtcatt
     9181 cccgcgcagg cgggaatcca ttgataaaac tcagttcatc agatttaaac agcgattgcc
     9241 caaattcaga aatggattcc cgcctgcgcg ggaatgacgc caaatgatta tttttcggtt
     9301 gcaacacgct gtttatcaaa aacaggtcgt ctgaaaacct gattttctgt tttcagagtg
     9361 cgaagcctgc ccgtgggtac gaccttttcg atttatttgc ccaatgcgcc caatacttcg
     9421 gctttcacgg cttctaccgg ctgagtgccg tcaactttga tgtatttagg cgcgtgttcg
     9481 ccttccagtt tgctgtaaaa atcgaccaaa acttcggttt gctcgtggta aacggcaagg
     9541 cgttttttca cggtttcttc tttgtcgtcg tcgcgctgaa tcaaatcttc gccggttacg
     9601 tcgtctttgc cttcaacttt gggcgggttg taggtaacgt ggtaagtacg gccggaagcc
     9661 aaatgcacgc ggcggccgct catgcggtcg acaatcacgc tgtcaggcac atcgatttca
     9721 acgactgcat ccaaatccac gcctgcttca accatcgctt cggcttgtgc caatgtgcgc
     9781 gggaaaccgt caaacaggaa accgtttttg cagtcgtctt gcgcgatgcg ttctttgacc
     9841 atgccgataa tgatgtcgtc gcgcaccaag ccgccttcgt caatgatttt tttcgcttcc
     9901 aaacccaagg gcgtgcctgc cttaatcgcg gcacggagca tgtcgccggt agagatttgc
     9961 ggaatgccga acgctgcggt gatgaattgc gcctgagtgc ctttgcccgc gcccggtgcg
    10021 cctaaaagta atgctttcat gaagtgatcc ttttaaatgt gtttattgga atcaaacaga
    10081 tggtttacga ttctacacgg cttggcacaa tagttcaaaa accaactgca caaactgcca
    10141 tttgcaagca aatgttttgc atacctttgc cgagcctgat attcacaagg cggcaacttt
    10201 ttgggacaat ccccaaaatt tcaaaacatc acaccctacc aaaaaggaac atccgtgaaa
    10261 cgcatttttc tgtttttggc taccaatatc gctgttttgg tcgtaatcaa cattgttttg
    10321 gcggttctgg gcatcaacag ccggggcggc gcgggcagcc tgttggcgta ttccgccgtc
    10381 gtcggcttca gcggctcgat tatttcgctg ctgatgtcca aattcatcgc caaacaatcg
    10441 gtcggtgcgg aagtcatcga cacgccgcgc accgaagaag aagcctggct tttgaacact
    10501 gtcgaagccc aagcgcggca atggaacctg aaaacgcccg aagtcgccat ctaccactcc
    10561 cccgaaccca atgcctttgc cacgggcgca tcgagaaaca gctccctgat cgccgtcagc
    10621 accggtttgc tcgaccatat gacgcgtgac gaagtggaag ccgtattggc gcacgaaatg
    10681 gcacacgtcg gcaacggcga tatggttacg ctgacgctga ttcaaggcgt ggtcaatacc
    10741 tttgtcgtgt tcctgtcgcg cattattgcc aacctgattg cccgaaacaa cgacggcagc
    10801 cagtcccagg gaacttattt cctggtcagc atggtattcc aaatcctgtt cggcttcctt
    10861 gccagcttaa ttgtcatgtg gttcagccga caacgcgaat accgcgccga cgcgggcgcg
    10921 gcaaaactgg tcggcgcgcc gaaaatgatt tccgccctgc aaaggcttaa aggcaacccg
    10981 gtcgatttgc ccgaagaaat gaacgcaatg ggcatcgccg gagatacgcg cgactccctg
    11041 ctcagcaccc acccttcgct ggacaaccga atcgcccgcc tcaaatcgct ttaaaccgat
    11101 ttgaaacggc aaaaaccgta cgcgcaagca gtacggtttt ttgtatatgc tgtctgaaaa
    11161 catcaggttt ccataccgac accaaccgta tccatttccg caccggatac gccgcattga
    11221 tataccctga taaaccgtat ccggacagaa a
//
LOCUS       DAXHTR010000067        10686 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.067, whole genome shotgun sequence.
ACCESSION   DAXHTR010000067 DAXHTR010000000
VERSION     DAXHTR010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.067"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(64..1206)
                     /locus_tag="ACFNML_001199"
     CDS             complement(64..1206)
                     /locus_tag="ACFNML_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HFC6392586.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELESLGCTDVQVFDGGVSCRGGL
                     EQVYAANLHSRTASRILLRLTKGTYRNERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLDERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLSAGYDGTQPFQDPFCGSGTIAIEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWADLRRRAEAQTRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFGVADAQSVRPNGENGIMVSNPPYGVRLEEVHALQALYPQLGTWLKKHYAG
                     WRVGMFTGDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(1255..1677)
                     /locus_tag="ACFNML_001200"
     CDS             complement(1255..1677)
                     /locus_tag="ACFNML_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392587.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPAGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            complement(1670..2479)
                     /locus_tag="ACFNML_001201"
     CDS             complement(1670..2479)
                     /locus_tag="ACFNML_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6392588.1"
                     /translation="MIQDTRPLIRVVAGILLNRDGDYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALRREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQEWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEGSGANVLMEAAQWQDRPEHADSVWMVVQTREQWRQAQEKGADAVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            complement(2561..2938)
                     /gene="acpS"
                     /locus_tag="ACFNML_001202"
     CDS             complement(2561..2938)
                     /gene="acpS"
                     /locus_tag="ACFNML_001202"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFC6392589.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAVRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            complement(3138..3905)
                     /locus_tag="ACFNML_001203"
     CDS             complement(3138..3905)
                     /locus_tag="ACFNML_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6392590.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMILFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIAKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            complement(3915..4694)
                     /locus_tag="ACFNML_001204"
     CDS             complement(3915..4694)
                     /locus_tag="ACFNML_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HFC6392591.1"
                     /translation="MNQKYILSANNNSLIEEIHNTVQRIGYCIVRGLNLNHLDDSRRN
                     KKLFDFLSQLGMLTNHKDDGFKSIFWDIKYRGDDYVINNDITFSEDVGECPLHSDSSF
                     SENPESYLVMYVVKSANDGGNSLFLSSSDIVNQLSKTETGKKHLKTLTGNLYPFKTPA
                     SFDKKQGVRWGNILSVNTQMIRFRSDCIYKGIEENRNKVSKEMVLALDYLINVIKNAS
                     DIQEFSAQDDGLIIIDNVNGLHARTDYTDKNRHYIRARITV"
     gene            complement(4741..5469)
                     /gene="pdxJ"
                     /locus_tag="ACFNML_001205"
     CDS             complement(4741..5469)
                     /gene="pdxJ"
                     /locus_tag="ACFNML_001205"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFC6392592.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERIQNGAHFASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLISQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            complement(5493..6284)
                     /gene="recO"
                     /locus_tag="ACFNML_001206"
     CDS             complement(5493..6284)
                     /gene="recO"
                     /locus_tag="ACFNML_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFC6392593.1"
                     /translation="MSEHRVNHEPVFLLASSPWRESSLRVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSASWYGSQELKTLHRAEWIGGWPQPQGRALFSGLYVNELMLK
                     LTVREDPLPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            complement(6318..7445)
                     /gene="pheA"
                     /locus_tag="ACFNML_001207"
     CDS             complement(6318..7445)
                     /gene="pheA"
                     /locus_tag="ACFNML_001207"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HFC6392594.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDNCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEVVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            complement(7484..8716)
                     /locus_tag="ACFNML_001208"
     CDS             complement(7484..8716)
                     /locus_tag="ACFNML_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HFC6392595.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFASSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQHFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHNGSATVMAVTMTASTSCGIAL
                     LWLCSHRAWKENEQSEYL"
     gene            complement(9236..10090)
                     /locus_tag="ACFNML_001209"
     CDS             complement(9236..10090)
                     /locus_tag="ACFNML_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HFC6392596.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
     gene            10348..10686
                     /locus_tag="ACFNML_001210"
                     /pseudo
     CDS             10348..10686
                     /locus_tag="ACFNML_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581361.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
BASE COUNT         2581 a         2905 c         2754 g         2446 t
ORIGIN      
        1 atcggccgga tattaaaaaa agccgtctga acatttttca gacggcattt ttgtacactt
       61 tcctcaacgg ttcgatccct gcaccatatc aatcaggaac aggcggcaat cgaggttgcc
      121 gttataaagc ggaattttgc gttttggcga caggcgcatg aatttgggca tttccctgtc
      181 gccggtaaac atgccgaccc gccagccggc atagtgtttt ttcaaccacg tacccaactg
      241 cggataaagc gcctgcaaag cgtggacttc ctcaaggcgc acgccgtagg gcggattgga
      301 caccataatg ccgttttcgc cgttcggtcg gacggactgc gcgtcggcaa cgccgaagga
      361 aacgatgtcg tccacttcgg cactgcgtgc gttgtccaat gccgtctgaa cgatgcggcg
      421 gtcgctgtcg ctgcctgcaa tcggggcgcg gacggggcgg gtttgcgctt cggcgcggcg
      481 gcgcaaatcc gcccacagcg ttttatcgaa attttgcagt ttttcaaaac cgaagcggcg
      541 catcatgccc ggtgcgcggc ctgtggcaat ccaagcggct tcgatagcaa tcgtgccgct
      601 gccgcaaaac gggtcttgaa acggctgcgt gccgtcgtag cctgccgaga gcagcagtcc
      661 ggcggcaagg ttttcgcgca gcggggcttc gccggtatcg agacggtagc cgcgtttgaa
      721 cagggcttcg cccgaagtgt cgatgaagat ttcgacattg cgttcgtcca aaaaggcgtg
      781 gatgcgaaca tcgggcgcgg ctttgtccac gctcggacgt gcgtcgtaaa tgtcgcggaa
      841 agcgtcgcag acggcatctt tgacggtcag tccgacaaat tggatgctct taacgttggc
      901 acgctttgcc tcgactttga ctttgaacgt ctgctgtaaa gtaaaccaat taaaccagtt
      961 gatatttttg gcgagtttgt agatgtcgcg ctcattgcgg tatgtccctt tggtcaggcg
     1021 cagcaggata cggctggcag tacgcgaatg caggttggcg gcgtaaacct gttccaatcc
     1081 gccccggcag gaaacgccgc cgtcaaacac ttgtacatcg gtacagccga ggctttcgag
     1141 ttcttgagat aaaacggtct ccaagccgcg cgggcaggtg gcgaaaagtg tatataaagt
     1201 attcataacg tgtcctttcc ggacggatgt tccggcaatg gcggattgaa aggtttattc
     1261 ggcggtaaat gccgtctgaa atgtccgact gccgattgta atgtccgccg taaaatcgcg
     1321 cctgccttcg acacagatgg gcaggcggat gcgtactgcc tgccaagtcc ccgacggttg
     1381 ccgctcgaac atatagcggt tgaaacccat atccatattt ttcatactga agctgatgct
     1441 gacctgttcc gtgccggcgg gcgcgtgttc gatataaata tcaaacggtt ttttggttga
     1501 aacggcggcg gcgcggacgc ggcttccgtc cggcagcgtg caaccctcgg tcaaatcgca
     1561 ttgcgccgcc acagcttgcg gctgctgcgc ctgccaccat tgcaacaaaa cgagcttgac
     1621 ggcggcaaag gcaatcagca gcagtgcggc aagcagtaat ttacggtttt tattcatgat
     1681 tcttccctat tgtttcatcc cttaccacca cgtgcgcccc caatccgagc catagttttc
     1741 cataacgtgc aaccgtctgt ccgtttgctg caagtacgag cggcacggat acgccctgcc
     1801 gcagggcttc tgccgcctct tgtgcctgaa catcatcgca cacgcgccaa acgaccgcat
     1861 ccgcgccctt ttcctgcgcc tgccgccatt gttcgcgggt ctgcaccacc atccacacgc
     1921 tgtcggcgtg ttcgggtctg tcctgccatt gcgccgcctc catcaaaacg ttcgcaccgc
     1981 ttccctctgc cgaacccaaa ggcaggacgc ggtacgcgcc catactgttc tccccgtgca
     2041 aacccgtttt caggctgccg tacaaacggc gcggaacgga cagcgaacgc aaaagcgcgc
     2101 cgttggcggg cagcatgggg gcaacggtaa aatcacccgc cttctgccaa gaccattcct
     2161 gcccttcgcg ggattgcggt tcgcccgtcc attggccggg gttgacccat aggaatttca
     2221 ggcagacgcg ggcgtgttcg taggaatgga ttttggtcag ccaaggcgtg gcggcgagga
     2281 tgcggatgcc gagttcttct tcaaactcgc gccgcagggc ttggaagtcg gtttcgcccg
     2341 cttcgacctt gccgccggca aattcccaat atccggcata gggtttgcct tcggggcggg
     2401 aactgagcag gtagtcgccg tctcggttga gcaggatgcc ggcaacgacg cggataaggg
     2461 gtcgggtgtc ttgaatcatg gcgggtacgc ggttggtcgg tcggagtgca aatgagggac
     2521 gggcaatgcc gtcaacgggt ttgccgcatt tcagacggca ttatttttcg gcaacgacaa
     2581 acgccaatac ggtgtcttct tcgtcgctca tgctgaggct gacgcggctg atgccttgtt
     2641 cctccagcca tttggacagg gcggggccgt agaaaaattc gggcttgccc aatgcgtcat
     2701 gcccgatgcc gatgttgcgg aaggaaaccg cgccgcgtat gcccgtgccg acggctttgg
     2761 caaaggcttc tttggcggca aagcgtttgg caaggtagtt gacgggtttg cccgcctgag
     2821 gaaattcaag cagctcttcc ggagtgagga tacgcacggc aaacgcctgt ccgaattttt
     2881 tgtttaagcg gatgatgcgc ttgagggaaa cgatgtctgt accgatgccg taaatcatat
     2941 ttgcgctcct tcgcccttgg ttgcgggtag tgatgaatgt gatgatgaat gccgtcaatt
     3001 tcttggggcg ttaaaatggc gtgaatatcc actttcgggg ttttgtcggt aacaattttt
     3061 attgtaacct tttgcatttg aaattctaaa acatcgtgcc aattatttaa taaggagtta
     3121 aggcatttaa taatgaatta aagtttgatt aaacccgaat gcccgatttt cagagccaat
     3181 accaataaaa caataaaaat taacatttta aaaaaatttg ggctaatctt agtccttaac
     3241 cgaattccaa catacaatcc aataacagac aatacggaca gtaaaaatat taaaccgtat
     3301 tcactcttat ttaataacca atactggtct cttagcatat atatttgaac aattttcgcc
     3361 aaaagatagc atagattgct tgattttgcg atacgatttt tattctctgt ttcgctaagc
     3421 aaaaatatta acaatatggg agacatggca ttggttgaac cgccgatgat gcctgccaaa
     3481 aacccaaaaa gaatcatatt cttattattg gcaactactt gaatattttt tgcttttgca
     3541 catacattta aaataccatt aacagaataa tacaatgtaa tgattgccat cagtaaaagc
     3601 agccacgaca ctggaagtat caaaagcaac ttcaccccca aaatgctgcc aacgacgctg
     3661 ccgatagcaa gcaatttata ggtttttaaa taataaacaa tctcttgcca aaaacccttt
     3721 ttgttattgc tgcatagaac caacaagctc attaacaggc ttggtaatgc caccaaggca
     3781 acaaccttag acaatggcat gataaaagcc aatgcggttg taccgagcat cggaaatccc
     3841 atgcctgtaa ttccgtgcag tattgcggca gcaacaaaaa cgatagattg cattatttct
     3901 tgcataaccg tcctttatac agtaattctt gctctaatat aatgcctgtt tttatccgta
     3961 taatcagttc tggcatgcaa gccattgaca ttgtcaataa taatcaaacc atcatcttgt
     4021 gcagaaaatt cttgaatatc actcgcattt tttataacat ttataagata atcaagtgca
     4081 agtaccattt cctttgatac tttatttcta ttttcttcaa tacctttata gatacaatca
     4141 cttctaaatc taatcatttg agtattgacc gataagatat taccccatct cacaccttgt
     4201 tttttatcaa atgatgctgg tgttttaaat ggatataaat tgcccgttaa tgtttttaag
     4261 tgttttttac cggtttctgt tttagataac tgattgacaa tatctgatga acttaaaaat
     4321 agggaattac ctccatcatt ggctgatttt actacataca taaccaaata actttccggg
     4381 ttttcactaa aagatgaatc actatgaagt ggacattctc caacatcttc cgagaaagtt
     4441 atatcattat ttattacata gtcatcgccg cgatatttaa tatcccaaaa tatagattta
     4501 aaaccatcgt ctttgtggtt tgtcagcatt cctaattgag atagaaagtc aaataatttc
     4561 ttgtttctcc ggctgtcatc aagatggttt agattaagac cgcgaacaat acaataccca
     4621 atacgctgta ctgtattgtg aatttcttct atcaaactat tattgtttgc agataaaata
     4681 tacttttgat tcataacata ctcctgtcgt gaaatgccgt ctgaaacggt ttgcctgccc
     4741 ttacggcagc agccttgccc tgaacatcgc ctccttcatt tggcgcacgg cctcgggcaa
     4801 tccgaggaaa agtgcctgcg aaatcagcga atgcccgatg ttcagttcgc ggatggcgag
     4861 gatttgggcg atgggggtaa cgttgtgtat ggtcagtccg tgtccggcgt tgacgaccaa
     4921 gcccaaatcg ctggcaaaat gcgcgccgtt ttggatgcgc tcgaactgcc tgatttgttc
     4981 ggcgtggctg cgcgcgtcgg catacgcgcc tgtgtgcagc tcgacaacgg gcgcgccgac
     5041 atcacgggcg gcttggattt gcctgtcgtc ggcatcgata aacaaggaca cgcgtatgcc
     5101 cgcgtcggtc aggattttgg tgaattcggc aattttgtct tgttgcgcca atacgtccaa
     5161 gccgccttcg gtcgtgattt cctgacgttt ttccggcacg atgcacacgt cttccggcat
     5221 cactttaagc gcgttttcga gcatttcttc cgtcaacgcc atttcaaggt tcaggcgcgt
     5281 gcggatggcg tttttgacag caaacacgtc cgcgtctttg atgtggcggc ggtcttcgcg
     5341 caggtgcatg gtaatcaggt cggcaccgtg cgtttcggca accagtgccg cctccacggg
     5401 gctgggataa gtcgtgccgc gcgcattgcg gacggtagcg atgtggtcga tgttgacacc
     5461 taaaagcata atctttcctt ttatttctgc cttcagacgg catttgaagc cgtgccgtcc
     5521 gaagtcggga cggtttcccg ggcggtttct ttgcggtcaa actgccgtat ctgttccaac
     5581 acctgccgcg atttcagccc ctcgggcaac aggtggcgga taaaaagccg tgtgattttc
     5641 aatgcctgtt gcaggctttc ggcagtgcgg aaactgcctt cgcgcaaatc gatcaggctc
     5701 tgcccggggg cgacaacgcc ggcggcgtgc ggcggtacgg caaatccttt tccgacgggg
     5761 aagacggctg tttccgggcg gacaaggtat gtgccgcctg ccgcaatcgt cccgccgtcc
     5821 ccgtcgcggt tcaaatcggg ggcaacgccc aacaggttca gcagccgcca ctcgaaacgg
     5881 cgcaagtcgt cgatataagc ggctttgcag cacaccgcct ccatcacttc cgccaacgcg
     5941 tcgtataact cgggcaacgg atcttcgcgg acggtcaact tcagcatcaa ctcgttcaca
     6001 tacaatccgc tgaacaacgc cctaccctgc ggctgcggcc acccgcctat ccattcggcg
     6061 cgatgtaggg tcttcaactc ttgcgaacca taccaagacg cactcaccgg cacaaacggc
     6121 accaacacgc cgcgcaattc gctctgccgt ttgcgcgcgc ttcttgccaa aagtgccaca
     6181 cgcccgtaac ggcggctgaa cgcttcgacc cgcaggctgc tttcgcgcca gggcgaggaa
     6241 gccaacagga agacgggttc gtggttgact cggtgttcgg acatcatgcc tgaaatgccg
     6301 tctgaacgct gccgccgcta caaaacggcg gtcgggtacg aaccgatgac tttgacgaac
     6361 gaagcgcgtt cgcccaagcg ttccaatgcc gtctgaatct gcgcgtcccg gcggtgtcct
     6421 tcgatgtcga tgaagaacag gtattcccac aaaacggatt tactcggacg gctctcaaac
     6481 ttggtcatgg aaatacccga ctcagtcagc ggttgcagca gcgaggcaac cgcgcctgcc
     6541 cggttgggcg cggaaacggc cagcgaagtc ttgtcgctgc cgcttgcacc ggtttcgtga
     6601 tgtcccatca ccaagaagcg cgtggtgttg ttcggttcgt cttcgatgca ctcggcaacc
     6661 atatcgagtc cgtagatttc cgccgccgtg cgtccggcga tggcggcaac cgtaccgtcg
     6721 tccgattcgg caaccagcct tgcggcttcg gcattgctgg acacggcaat ccgttcggcg
     6781 ttgggcaggt gtctgcccaa ccagtcgttg cactgcgcca acgcctgcgc gtgggaaaag
     6841 actttggcaa tgccttcggt gctgccgttg tttttacgca aaaggttgtg gtggatgcgc
     6901 aaaacgactt cgccgcacgc ctgcaacgcg gtaacggcaa gcaaatccaa cgtgcgcccg
     6961 accgagcctt cggtcgagtt ttccacgggg gcgaccagat agtctgcctg acgggtttca
     7021 acctgcttga agcagttgtc tatggtcgga cacgccatgg tgtgcgcggc gtgtccgaaa
     7081 tgcttaatcg ccgcctgctg ggtaaacgtg ccctgcggcc ccagataggc gatggtcagc
     7141 gggcgttcga cggcgaggca ctcgctcatc acttcccgaa acaggcgtgc taccgattcg
     7201 tcgggcagcg ggcctttgtt caaatcctga atgcggcgca acacggcgac ctcgcgctcc
     7261 ggacggtata ctgcgcccgt gcctttcagt tcgccgatgg cgtgggcgtg ttgcgcacgt
     7321 tcgttgagca ggcgcaggat ttcggcatcg atggtgtcga tggcgttgcg gtggggaagg
     7381 aggagttcgt cgatagtttg ggacataatt tcgcctgaac agtaatttat cgtgcattct
     7441 aacatcaaat gccatctgaa acggtttcag acggcatttg atgttaaagg tattcgctct
     7501 gttcgttttc tttccacgca cgatgtgagc agagccacag aagcgcaatg ccgcaagagg
     7561 tggacgcggt catggttacc gccatcacgg ttgccgaacc gttgtgcaag aaggtcgccg
     7621 ccatacccac gcccgcgccg attaaggact ggaacacgcc caacacggcg ttcgcgctgc
     7681 cgccctcttc tttgaaataa gacataaagc atgcctgcgt gttcgcgccg accaagccct
     7741 gcgtgccgac ggaaaacatc acgcacgcaa ccagcagcca aaacgggggc aacccgaaaa
     7801 acagcacggc ggcgagttgg gacaggttgg cggcaaattg gacgacaatc ccccatagca
     7861 ggatgctttg cggatgcacg ccggttttga gccgccacgc ggtaacgcgg ttgaaaaaca
     7921 tcatcgtgat gatgttgagt gcaaacgccc aagcgtattg atgaggcgta acacggtaaa
     7981 gctgctggta cacgaaggaa gattcggtca gaaaggcgaa catcgaaccg aagctgaatg
     8041 cctgaaaaaa cagataaccc atcgcggcac gggttttcaa tacgcgcttg aaccgccccg
     8101 ccaccagccc gaacacgtcc cgtccgattt tgccgccgac ggcaggcttg ggcaggaaat
     8161 gctgcaccaa accgagcagc accagcgaat acgccgccag aaaaacaaaa atcgcctgcc
     8221 agccgcccaa gccctgcaac aatgcgccga ccatgggtgc gaccagcggc acaaccatca
     8281 aaatgatgcc gataagggca aacatctggg cggctttgcg tccggaataa taatcgcgca
     8341 ccatcgcgcc gacgatgacc acagtcatgc ccgcaccgaa tgcctgcacg acacgcaggt
     8401 tgaggagctg ttcggcactc gaagcaaata cgatggcggc aacggcaagg caatatacaa
     8461 tcagaccggt cagggcgacg ggtttgcgcc ctttgatgtc ggacaccgaa ccgccgacca
     8521 cctgtccgaa cgccgtgccg aacataaaca aactcaggct ctgttcgatg cggtggacat
     8581 ccgcgttcag cgactgcgcc atttcgggaa tcgcgggcag gtaggcatcg atggaaaacg
     8641 gcatcagcgt aaccagcatc gccatcaaaa ccgccatcag tttttcgctc atttcaggat
     8701 aatgggcaga aggcataaat tctcgtccga ttcgcaaaca aaccgcccgg cggcggaaaa
     8761 cacaatacgc ggaaacggaa aattttaaca caccggcaca aacaggcggc aacaaaagcc
     8821 ccggcaaaac cggacgaacc ggaacgatat agtggattaa caaaaaccag tacagcgttg
     8881 ccttgcctta gctcaaagag aacgattctc taaggtgctt aagcacccag tgaatcggtt
     8941 ccgtactatc tgtactgtct gcggcttcgt cgtcttgtcc tgatttttgt taatccgcta
     9001 taaaatcccg tcattcctgc gcaggcggga atccggacat ccaacgctgc agcaatttgt
     9061 cggaaataac cgaaactcaa aaaaccggat gcccgcctgc gcgggaatga cgatgcaaag
     9121 atggtcgtta tttcggataa atatccgtct tttcgttccg gatacccgct ttcgcaggaa
     9181 tggagatgga aagatttcag cctattcggc aatcatcaaa taccgcttcc caactttatt
     9241 tttgatgcga cagccggccg gcaaccaagg tggcggcgat gacgacaaac acgcccaagg
     9301 cggacacggg cgacaggtgt tcgcccaaaa tcaaaaccgc cagcagcacg ccgacgacgg
     9361 gttcgagcga aatcaacagt cccgaaacgt tggcaggaac acggctcatc cccttgttcc
     9421 acagccaata ggcgtaccag ccgcacccca aacccaaata cagcagcgac aataccatcc
     9481 cgacgctcca gtccacggta taactttgcg ccaaagcaag cgaaaacggc aggcacatca
     9541 acgatgcggc ggcaatggaa acagatgtga atgccggtgc gccgatgcgt gcaatcagcc
     9601 tttgcgtcgg acgcatagcg gcacaaaagc ccgcgcccgc caacaacacc agcaggcagc
     9661 cgaaccagcc gacttcgccg ccctcttccg caccgcccgc catcagcagc gcgacaccgg
     9721 caaatgccgc cgcgccgcat atccagtggt aggcacgcgc tttgtcgttg aagaaaaagt
     9781 gtccgacaaa caccatcagc agtggctcga gtccgacaat gaccgatgcg ctggcggcgg
     9841 aagtgtattt caacccgaca aactgaagca gcagggtcag cacatagttg acgaacgaca
     9901 caatcagcaa cggcttccat tcctcacgcg gaatcttgcc gacatgacgg cggcaggcgg
     9961 gcagtgcagg cagcgcggca attagcaggc gcacgccgac catcaatgcg ggatcgatgc
    10021 cgccatagac atatttggcg gcaataaacg agctgctcca gataatcagg gcaaggattt
    10081 ggtaaaacat agtgagtgat aaagtcagat ggtcatacaa aagtatagcg gatgaacaaa
    10141 aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacgaaaccg attcacttgg
    10201 tgcttcagca ccttagagaa tcgttctctt ttttgttcat ccgctatatt gtgttgaaac
    10261 atcgccacaa acctgatata gtccgctcct gcaacatcag tgaaaatcgt tctttttaat
    10321 cagttaaaac cgaatacgga gtcgaaaatg aatccagccc ccaaaaaact ttctcttctc
    10381 ttctcttctc ttctcttctc ttctcttctc ttctcttctc ttccgcagcg caggcggcaa
    10441 gtgaagacgg cagccgcagc ccgtattatg tgcaggcgga tttagcctat gccgccgaac
    10501 gcattaccca caattatccg gaaccaaccg gtgcagacaa agacaaaata agcacagtaa
    10561 gcgattattt cagaaacatc cgtgcgcatt ccatccaccc tcgggtgtcg gtcggctacg
    10621 acttcggcga ctggagaata gcggcagatt atgccagtta cagaaaatgg aaagaaagta
    10681 attctt
//
LOCUS       DAXHTR010000068        10608 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.068, whole genome shotgun sequence.
ACCESSION   DAXHTR010000068 DAXHTR010000000
VERSION     DAXHTR010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.068"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(119..1336)
                     /locus_tag="ACFNML_001211"
     CDS             complement(119..1336)
                     /locus_tag="ACFNML_001211"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HFC6392597.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAVS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(1538..2266)
                     /gene="rph"
                     /locus_tag="ACFNML_001212"
     CDS             complement(1538..2266)
                     /gene="rph"
                     /locus_tag="ACFNML_001212"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFC6392598.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHTDGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(2348..2812)
                     /locus_tag="ACFNML_001213"
     CDS             complement(2348..2812)
                     /locus_tag="ACFNML_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HFC6392599.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNGARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3029..3805
                     /locus_tag="ACFNML_001214"
     CDS             3029..3805
                     /locus_tag="ACFNML_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HFC6392600.1"
                     /translation="MSDNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKANMRVPCVYSTENIVPPQSREAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIADKK"
     gene            3943..4470
                     /locus_tag="ACFNML_001215"
     CDS             3943..4470
                     /locus_tag="ACFNML_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392601.1"
                     /translation="MEFVNNLVIFSFLLLMLIPIFFVVYGIYHKIRYRKIYILRISFT
                     LLVVILCSMYYIYCRYLDQQKVAYYCIDEQCISIVHLYKDYGINSPTYARIYAGKILF
                     RFQVRAKNYAELLMEDDISISKKILGNKFIIYGSLPVIYGNVDNIEVKEATGYIDRAS
                     TDYIVSRNLKFRHLY"
     gene            4641..5495
                     /locus_tag="ACFNML_001216"
     CDS             4641..5495
                     /locus_tag="ACFNML_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HFC6392602.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5536..6744
                     /gene="pncB"
                     /locus_tag="ACFNML_001217"
     CDS             5536..6744
                     /gene="pncB"
                     /locus_tag="ACFNML_001217"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFC6392603.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCRLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEVGTD
                     DKGRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAARLKEI
                     AAAQNPDEPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYVWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6836..8554
                     /gene="argS"
                     /locus_tag="ACFNML_001218"
     CDS             6836..8554
                     /gene="argS"
                     /locus_tag="ACFNML_001218"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFC6392604.1"
                     /translation="MNLHQTVEREAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVADALAGNAVIESAEVAGPGFINLRLRPEFLAQNIQTALNDA
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVSEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEDPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKTGEWDADAPTVLTEPLEKQLAAELLKFEDVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9093..10511
                     /locus_tag="ACFNML_001219"
     CDS             9093..10511
                     /locus_tag="ACFNML_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFC6392605.1"
                     /translation="MNIHTLLSKQWTLPPFLPKRLLLSLLILLAPNAVFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIVGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPAPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVVAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGKM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHSKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGTEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2526 a         3013 c         2610 g         2459 t
ORIGIN      
        1 ttaatccact atattttttg ttccaaagtc aaaatatgcc gtccgaacat tcgggcggca
       61 gacaaaacgg cactgcccga taaaggcagt gccgttgtcc gtttcaaacc gtgaaacatc
      121 agcccaaatt aaaggcttta tgcaataccc tggttgccag ttccatgtat ttttcatcaa
      181 tcaatacgga aactttgatt tcggaggtgg aaatcatttg gatgttgata ccctcttcgg
      241 cgagcgtgcg gaagattttg gcggctacac cgacgtgcga acgcataccc aaaccgactg
      301 cagagacttt gcatacggtg tcgtcgccat caatagaaac cgcgccgata ctgtcttggc
      361 gttccgacag gatttccaaa gtctgcttgt aatcgccgcg cggtacggta aaggaaaaat
      421 cggttgtgcc ttcgctgccg acattttgga taatcatatc gacttcgatg ttggcatcgg
      481 caaccgcgcc taaaatctga taggcgacac cgggtttgtc gggtacgccg cgcacgttga
      541 tgcgggcttg gtttttatcg aatgcgatac cggttacggc agctctttcc atgttgtcgt
      601 cctcttcaaa ggtaattaag gtgccattgc cgccgtcttg caggctgctc agtacgcgca
      661 ggcgcacttt gtattttccg gcgaattcta ctgaacggat ttgcaaaact ttcgaaccga
      721 ggcttgccag ttcgatcatt tcttcaaatg taaccgtatc catgcggcgc gcttcgggta
      781 cgacgcgggg gtcggttgtg taaacgccgt ctacgtcggt atagatttgg cactcgtcgg
      841 ctttgagggc tgcggcaagc gcaacggcgg aagtgtcgga accgccgcgt ccgagcgtgg
      901 aaatatcgcc ttcactgctg atgccttgga agccggcaac gatgacgact ttgcctgcat
      961 cgaggtcggc acgcattttt tcgtcatcaa tgctttcgat gcgggctttg gtgtgggcgg
     1021 tatcggtttt gagggcgacc tgccagcctg tgtagctttt ggcatccacg ccgatgtctt
     1081 tcaatgccat cgccaaaagg ccgatggtta cttgttcgcc ggtggacaaa accacgtcca
     1141 attcgcgcgg atcgggatgc tcctgcattt cgtgcgccaa cgcgaccagg cggttggttt
     1201 caccgctcat ggcggatacg acgacgacga tgtcgtgtcc ttcggcgcgg gctttggcga
     1261 cacgtttggc cacgttttta atgcgttcgg gcgagcctac ggatgtgccg ccgtatttgt
     1321 gtacgattaa cgccatgttt cgtgctttct tgtgtgggat tggtcgggca gcatggtttg
     1381 ctggaaaagc ggctattatt actatttttt acatgaagtt caagaacgga ctgcgctttc
     1441 ccgcctgccg tttgacagcg gtcagcgaaa aacctgttct ttcagattgt tgacaaaatg
     1501 ccgtctgaac ggttttcaga cggcatccgg acgacaatca ggcggcggac aacgcatttt
     1561 gctggtgttg cagcagttcg cctatgcctt tttgcgccag tgcaaccagt ttgcccaatt
     1621 cgtccaaact gaacggcgcg tcttccgccg tcccctgtat ttcgatgatt tttcccgatg
     1681 cggtcatgac gatattcaca tcactgtcgc aaccggagtc ttcgggataa tccaaatcca
     1741 aaagcggcac gccgttcact acgcctactg acacagcggc aacggcttcg cggatggggt
     1801 tttcactcaa aatgccgtct gaaaccagtt tgccgacggc gatttgcagc gcgacaaacg
     1861 caccggtaat cgaagccgtg cgcgtaccgc catctgcctg aatcacatcg cagtcaatca
     1921 agatttgtcg ttcgccgagt ttttccatat ccacgaccgc gcgcagcgaa cgcccgataa
     1981 ggcgttggat ttcttgtgtg cgcccggact gtttgcccgc cgaagcttcg cggcgcatcc
     2041 gggaagcagt tgaggcaggc agcatcccgt attccgccgt tacccagcct tggtttttac
     2101 cgcgcagaaa cggcgggacg ttttcatcta tggaagcggt acaaatcact ttggtattgc
     2161 cgcattcaat aaggcacgaa ccgtccgtat gcggcaggaa atgaggggtg attttgatat
     2221 cgcgcaggct gtccgcggcg cgcgagatgc ggatgtaatc aggcatactg ccctcccgtt
     2281 aaaaacagat aaattaaaaa gccttaaata tgaaaaatca catttaaggc cttcaaactg
     2341 aaaatttcta cgcctcttcg gctttgctgc ggataatcaa aagcggcagg tggctttggc
     2401 gcattaccgt ttcggcaaaa ctgcccatta aaaggtgcat cagtccggta cgtccgtgcg
     2461 tgcccaacac cagcaggtcg gcaccgtttt catcggcata atcaaccaaa tcctgcgcca
     2521 tttcacgcgc acccttattg gcaaccagca ggtgtttgac ggtattttcc acacccagtt
     2581 cccgggcggt gcgctcggcg gcatccaaaa cttcgttgcc ttgcgcgacg gcggcggctt
     2641 cgtagctttc gtgttgcaaa aattcggggg cgagcgccat atattcggca ggattggcaa
     2701 cgtgtaccaa agtcagacgc gcaccgttga ccccggcaag ctcggcggca tgtttcaggg
     2761 cattgatgga cgtttcactg ccgtcaacgg caacaaccaa atgtttgtac atatcgtatt
     2821 ctccttttgc accgcctcgc ggtgccctct tgtcggatgg gcgcagggac agtttgcgct
     2881 gtttcattat agacccgccg tcgggcttta tacaacagcc gaacagcccg accgctttcc
     2941 agtataatat gccgcttccg tgcagtcagg cattttttgc cggctttcgt tcactttttg
     3001 atttgacgca atcttgcagg attcgaccat gtccgacaac gctttgacct cttcgcgacg
     3061 cttcggcggc atcgccagac tctacggaga ctctgccttg gcgcactttt cacaggcaca
     3121 cgtctgcgta gtcggcgtgg gcggtgtcgg ctcgtgggcg gtcgaggctt tggcgcggac
     3181 gggcatcgga cgtttgactt tgattgattt ggacaacgtt gccgaatcga atgtcaaccg
     3241 ccagctgcac gccctgaccg gagacttcgg caaagcaaaa gttaccgcct tgcgcgaacg
     3301 cgtcgcccaa atcaacccag aatgccaagt gtttgaaatt gaagatttcg ttaccgaaga
     3361 caatttgccg gaatacttcg gaaaaggttt tgatttcgtc atcgacgcga tcgaccaagt
     3421 gcgcgtcaaa gcagcaatgg cggcttattt tgtggaacgc aaacaaccgt ttgtcctcag
     3481 cggcggcgcg ggcggacaaa aaaatccggc gttaatccaa accgccgatt tgagccgcgt
     3541 aacccacgac ccgctgcttg ccaacctgcg ctacaccttg cggaaacgct acggattcag
     3601 ccgcgatacg aaagcaaata tgcgcgtgcc ttgcgtgtat tcgaccgaaa atatcgtgcc
     3661 gccgcagtct agggaggctt gttcggcaga tgccgctccg caaggcttgt cgtgcgccgg
     3721 ctacggtgca agcatgctcg ttaccgcttc gttcgggcta tattgcgcac aggcggcggt
     3781 ggaacacatc gcagacaaaa aataagcaat gccgtctgaa acaggattca gacggcattt
     3841 gaacaaacta tggttatgat ttaagacaac aaaggatacg gataaaaaat aacataaaat
     3901 atatgattcc taataatata ccaagtatcg gagagctatt taatggaatt cgttaataat
     3961 ttagttattt tttcattttt attactaatg cttattccga tattttttgt agtatatggt
     4021 atataccata agatacgtta tcgcaaaata tatatcctaa gaataagttt tacattatta
     4081 gtggtaatac tttgcagtat gtattacata tattgccgtt atcttgacca acaaaaagta
     4141 gcttattatt gcatagatga acaatgtatt tctattgttc atctatacaa agattatggt
     4201 ataaactctc ccacatatgc gagaatttac gcaggaaaaa tattgtttag atttcaagta
     4261 agagctaaaa attacgctga attacttatg gaagatgata tatcaattag taaaaaaatt
     4321 ttggggaata aatttatcat ttatgggtcg ctacctgtaa tatacggtaa tgtagataat
     4381 attgaagtaa aagaagctac tggttatata gatagagcca gtactgatta tattgtctca
     4441 agaaacttaa aattcagaca tttatattaa ttaagaggtt ttagcaagac cgtattcaca
     4501 acctgctcct tttcaaaaca tttgcattta aaagccgtta taatgccgtc tgaacatctg
     4561 cccgaccaca ttatgcgtga atgccggcag attgttttct tttgtaaact tatattaaaa
     4621 tccacttacc gattcacgcc atgccgccca tccctgcccc atccgcacca tccgagcaca
     4681 ctgtcgcatg ggtattcggc caacccgtta ccgatttgcc ccaggatttg tttattccgc
     4741 ccgatgcatt gaaagtcgta ttgggcagtt ttcaaggccc tttggatcta ctgctgtatc
     4801 tgatccgcaa gcagaacatc gatgttctcg atattccgat ggtgaagatt accgagcagt
     4861 atctgcacta catcgcccaa atagaaacct atcagtttga tttggcggcg gaatatcttt
     4921 tgatggcagc aatgctgatt gaaatcaaat cgcgcctgct gctgccgcgt accgaagccg
     4981 tcgaagacga agaagccgac ccgcgtgccg agttggtgcg ccgcctgttg gcttatgaac
     5041 agatgaagct ggcggcacaa gggttggatg cgcttcctcg tgcgggccgg gatttcgcgt
     5101 gggcgtacct gcctctggaa attgccgtcg aagccaagct gcccgaagtc tatattaccg
     5161 acttgacgca agcgtggctg ggtattttgt ctcgggcaaa acacacgcgc agccacgaag
     5221 taatcaaaga aaccatctcc gtgcgcgcgc aaatgacggc aatcctgcgc cgtttgaacg
     5281 gacacgggat atgcaggttt cacgacctgt tcaatcccga acagggcgcg gcttacgtgg
     5341 tcgtcaactt catcgcactg ttggagcttg ccaaagaagg tttggtcgga atcgtacagg
     5401 aagtcggttt cggagaaatc cgaatcagcc tcaatcatga gggggcacat tcagacggca
     5461 tttccggcac acggggcggg cgcgatgtgt tctaatacgc cccaagccgc caccaaaaat
     5521 cgggagacac gccatatgac cggcatcata cattcgctgc ttgacaccga cctctacaaa
     5581 ttcactatgc tgcaagtagt cctgcaccag tttccgcaga cgcacagcct ttacgaattc
     5641 cgctgccgca acgcctcgac cgtctatccg cttgccgaca tcaaagaaga cttggaagtc
     5701 gaactcgacg cgctctgccg gctgcgcttc acccacgacg aactcggcta tctgcgctcc
     5761 ctgcgtttca tcaaaagcga ctttgtcgat tatctcgaac tcttccagct ccaacgccgc
     5821 tttgtcgaag tcggcacaga cgataaaggc cgtctgaaca tccgcatcga aggtccgatg
     5881 atacaggcga tgttctttga aatcttcatc ctcgccattg tcaacgaact ttacttccgc
     5941 cgcctggaaa cccctgcagt catagaagaa ggcgaacgcc gccttcaagc caaagccgcg
     6001 cgcctcaaag aaatcgccgc cgcacaaaac cccgacgaac cgcccttcct gatttccgac
     6061 ttcggcacgc gccgccgcta caagctcgcg tggcaggaac acgtcatccg caccctgctc
     6121 gaagccgccc caagcatcgt gcgcggcacc agcaatgtct ttctcgccaa aaaactcggc
     6181 atcaccccca tcggcaccat ggcgcacgaa ttcctccaag ccttccaggc cctcgacgta
     6241 cgcctgcgga atttccaaaa ggccgcgctc gaaagctggg tgcacgaata ccggggcgat
     6301 ttgggcgttg ccctgaccga cgtggtcggt atggatgcct tcctgcgcga tttcgacctc
     6361 tatttcgcca aacttttcga cgggctgcgc cacgacagcg gcgaccctta cgtttggggc
     6421 gacaaagcct acgcccacta tcaaaagctc aaaatcgaca gccgcaccaa aatgctgacc
     6481 ttctccgacg ggctggacat cgaacgctct tgggcattac accaatattt caaagaccgc
     6541 ttcaaaaccg gcttcggcat cggcaccaac ctcaccaacg atatggggca tacgcctttg
     6601 aatatcgtgt tgaaactggt cgaatgcaac gggcagtccg tcgccaagct gtccgactct
     6661 ccgggcaaaa ccatgaccaa caacagcacc ttcctcgcct acctgcgcca agtgttcgac
     6721 gtacccgaac ccgaaacgcc gtaaaccggc agaaaaagcg cacaattcct gtttctgccg
     6781 cataaaatct tttaaaatac cgcctgattt gaatttaacc gaaagaccga acttcatgaa
     6841 cctacatcaa accgtcgaac gcgaagccgc cgccgccttt gccgccgcag gcatcgccga
     6901 cagtcccgtt gttttgcagc cgaccaaaaa tgccgaacac ggcgatttcc aaatcaacgg
     6961 cgtgatgggt gcggcgaaaa aagccaaaca aaacccgcgc gaactggcgc aaaaagtcgc
     7021 cgacgcattg gcaggcaacg ccgtcatcga aagcgcggaa gtcgccggtc cgggctttat
     7081 caacctgcgc ctgcgccccg aatttctcgc gcaaaacatt cagacggcct tgaacgacgc
     7141 acgtttcggc gtggcaaaaa ccgacaaacc gcaaaccgtc gttatcgact attcttcgcc
     7201 caatctggca aaagaaatgc acgtcggcca tctgcgttcc agcatcatcg gcgacagcat
     7261 ttcgcgcgtg tcagaattta tgggcaatac cgttatccgt caaaaccacg tcggcgactg
     7321 gggtacgcag tttggcatgt tggtcgctta tttggtcgag cagcaaaaag acaatgccgc
     7381 attcgagctg gcggatttgg agcagtttta ccgcgccgcc aaagtgcgct ttgacgaaga
     7441 ccccgccttt gccgacaccg cgcgcgaata cgttgtgaag ctgcaaggcg gcgatgaaac
     7501 cgttttggca ttgtggaaac agtttgtcga tatttcgctc tcgcacgccc aagccgttta
     7561 cgacacgctg ggcttgaagc tgcgccccga agacgtggcg ggcgaatcga aatacaacga
     7621 cgatttgcag cccgtagtcg atgatttggt tcaaaaaggt ctggcagtcg aagacgacgg
     7681 cgcaaaagtc gtgttcttgg acgagtttaa aaacaaagag ggcgaacctg ccgcatttat
     7741 cgtgcaaaaa caaggcggcg gcttccttta tgcttccacc gacttggcgt gtttacgcta
     7801 ccgtgtcggc acgcttcatg ccgaccgcct gctgtacgtc gtcgaccacc gccaagccct
     7861 gcactttgag caactcttta ccacttcacg caaagcaggc tatctgccgg aaaacgtcgg
     7921 cgcggcattt gtcggcttcg gcacgatgat gggcaaagac ggcaaaccgt tcaaaacacg
     7981 cagcggcgac actgtaaaac tggtcgatct gctgaccgaa gccgtcgaac gcgccaccgc
     8041 tttggtgaaa gagaaaaatc ccgaattggg cgcggacgaa gccgccaaaa tcggcaaaac
     8101 cgtcggcatc ggcgcggtca aatacgccga cttgagcaaa aaccgcacca gcgattatgt
     8161 gttcgactgg gacgctatgc tctcgtttga aggcaacacc gccccctatc tgcaatacgc
     8221 ctacacccgc gtgcaaagcg tgttccgcaa aacaggcgaa tgggatgcgg acgcgccaac
     8281 cgttttgacc gaaccgctgg aaaaacagct tgccgccgag ctgctgaaat ttgaagacgt
     8341 actgcaaagc gtggcggaca cggcgtatcc gcactacctc gccgcctacc tctatcaaat
     8401 tgcgaccctg ttcagccgct tctacgaagc ctgtccgata ctcaaagccg aaggcgcaag
     8461 ccgcaacagc cgcctgcaac tggcaaaact caccggcgac acgctgaaac aaggcttgga
     8521 tttgctgggc atcgatgtgt tggacgtaat gtaaaaccgc accgcccgat tgcggacaac
     8581 agcctcgcca tccttatccg aatctgaaaa aagcggcgcg atacaccgta tccgccgccc
     8641 ctcccaaaat gcgaaacaaa caaacgccaa gcaagcaagc aagcaagcaa gcaagcaagc
     8701 aaaaaattat aacccccttc ctgccgacgc acgcactttc cgcgcggcgc attccccttt
     8761 ttccgcccct caaacccgcc ttttcttcag gcagggtttc agcccgcccc ctttccctgt
     8821 tttcctttcc ccgacacgcg cgcgcgaccc ctgccgcatc gtgccgcact ttcgcgcccg
     8881 gacggcatcg ttccgccatc cggttctctg tttcaaatac ccctgtttca gaaagaaatt
     8941 ctagatgctt caacacacag gacgacatat aaagcgtcgc cctatatgtt gccctgattt
     9001 ggaaggggtt acgcctccca aataaagtct gatcctgccg ccccgaagga cagatgtccg
     9061 agtggcgaag tttcaaccga aaaggaaata cgatgaatat tcacaccctg ctctccaaac
     9121 aatggacgct gccgccattc ctgccgaaac ggctgctgct gtccctgctg atactgcttg
     9181 cccccaatgc ggtgttttgg gttttggcac tgctgaccgc caccgcccgc ccgattgtca
     9241 atttggacta ccttcccgcc gcgctgctga tcgccctgcc ttggcgtttc gtcaaaattg
     9301 tcggcgtatt ggcgttttgg ctggcggttt tgtttgacgg gctgatgatg gtgatccagc
     9361 ttttcccttt tatggacctc atcggcgcca tcaacctcgt ccccttcatc ctgaccgccc
     9421 ccgcccctta tcagataatg accgggctgt tgctgctgta tatgctggcg atgccgtttg
     9481 tgttgcagaa agccgccgcc aaaaccgact tccggcacat tgccgtctgc gccgccgtcg
     9541 tggtggcagc cggctatttc accggccatt tgagttacta cgaccggggg cggatggcca
     9601 atatcttcgg cgcaaacaac ttctattacg ccaaaagtca ggcgatgctc tacaccgtca
     9661 gccagaatgc cgactttatt accgccggcc tggtcgatcc cgtcttcctc cccttgggca
     9721 atcagcagcg tgccgccacg catctgaacg agccgaaatc tcaaaaaatc ctctttatcg
     9781 tcgccgaatc ttgggggctg ccggccaatc ccgagcttca aaacgccact tttgccaaac
     9841 tgctggcgca aaaagaccgt ttttcggttt gggaaagcgg cagttttccc tttatcggcg
     9901 cgacggtcga aggcaaaatg cgcgaactgt gcgcctacgg cggtttgcgc gggtttgcac
     9961 tgcgccgcgc gcccgacgaa aaatttgccc gctgcctccc caaccgtttg aaacaagaag
    10021 gttacgccac ctttgcgatg cacggcgcgg gcagttcgct ttacgaccgc ttcagctggt
    10081 atccgagggc gggctttcaa gaaatcaaaa ccgccgaaaa cctgatcggt aaaaaaacct
    10141 gcgccatttt cggcggcgtg tgcgacagcg agctgttcgg cgaagtgtcg gcatttttca
    10201 aaaaacacag caaaggattg ttttactgga tgacgctgac cagccacgcc gactatcccg
    10261 aatccgacat tttcaaccac aggctcaaat gcaccgaata cggcctgccc gccgaaaccg
    10321 acctctgccg caacttcagc ctgcacaccc aatttttcga ccaactggcg gatttaatcc
    10381 aacgccccga aatgaaaggc acggaagtca tcatcgtcgg cgaccatccg ccgcccgtcg
    10441 gcaacctcaa tgaaaccttc cgctatctca aacaggggca cgtcgcctgg ctgcacttca
    10501 aaatcaaata acaacaatgc cgtctgaacg caccaacagc cttcagacgg cattttgcag
    10561 acagaccgac ccctcaagcc cactttttca tcatttccga taaattgc
//
LOCUS       DAXHTR010000069        10599 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.069, whole genome shotgun sequence.
ACCESSION   DAXHTR010000069 DAXHTR010000000
VERSION     DAXHTR010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.069"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(271..2679)
                     /locus_tag="ACFNML_001220"
     CDS             complement(271..2679)
                     /locus_tag="ACFNML_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HFC6392606.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVRGSGEASIPEDYTRIVADKVEGQSQ
                     VQVRAEGNVVVERNRTTLNTDWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEQGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPLFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLYATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GATFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKDDAVMLDGSVGKKPRNRS
                     DWVAFASGSIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            2749..3753
                     /gene="amgK"
                     /locus_tag="ACFNML_001221"
     CDS             2749..3753
                     /gene="amgK"
                     /locus_tag="ACFNML_001221"
                     /EC_number="2.7.1.221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramate/N-acetylglucosamine kinase
                     AmgK"
                     /protein_id="HFC6392607.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGETAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARAAGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(3810..4508)
                     /locus_tag="ACFNML_001222"
     CDS             complement(3810..4508)
                     /locus_tag="ACFNML_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6392608.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRTEH
                     VVWQKEMLTLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQEPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMHTID
                     LLADKVREEQKAAQ"
     gene            4535..4726
                     /locus_tag="ACFNML_001223"
     CDS             4535..4726
                     /locus_tag="ACFNML_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392609.1"
                     /translation="MLEINRNPAILLHFRVLIQFAAGKGSFRFGKPLQTASNPMPSEA
                     VSVVQYAPLPQTGTTVEEQ"
     gene            4726..6264
                     /gene="murJ"
                     /locus_tag="ACFNML_001224"
     CDS             4726..6264
                     /gene="murJ"
                     /locus_tag="ACFNML_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFC6392610.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFGIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LSFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPG
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIAVGGGLYFASLAALGFRPRHFK
                     RVES"
     gene            6397..7353
                     /locus_tag="ACFNML_001225"
     CDS             6397..7353
                     /locus_tag="ACFNML_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HFC6392611.1"
                     /translation="MILTPPDTPFFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRSRGWHGAVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTLAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAANDPFLPPEALPTPDEVSEAV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            7404..8186
                     /gene="trpC"
                     /locus_tag="ACFNML_001226"
     CDS             7404..8186
                     /gene="trpC"
                     /locus_tag="ACFNML_001226"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HFC6392612.1"
                     /translation="MTDILNKILTAKAQEVAAQKAAVPLEHIRALAAEAAPVRSFIGS
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEREQLERFEAVA
                     HELGMTVLLELHDESELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRARGVHTFLIGETFMRADDIEAEVGKLF"
     gene            8251..10551
                     /gene="recQ"
                     /locus_tag="ACFNML_001227"
     CDS             8251..10551
                     /gene="recQ"
                     /locus_tag="ACFNML_001227"
                     /EC_number="5.6.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HFC6392613.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGRQEAVINTLAGGGSLTVLMPTGG
                     GKSLCYQIPALMCEGVAVVVSPLIALMNDQVANLHAAGIEAAAVNSGTSADGAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAASWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEESEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTAGFSRDAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLAATPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKRKRRLMRALIQWCGETAKHEQSEPYRILSKAALRAIAAKQPKGLAELA
                     AVYGVGEEKTARYGAAVLAVLGQNAV"
BASE COUNT         2144 a         3003 c         3060 g         2392 t
ORIGIN      
        1 ctttggcagc tttggcagct ttggcagctt tggcagcttt ggcagctttg gcagctttgg
       61 cagctttggc agctttggcg gatgcggttt ttgccttttg cggtgcggcg tggacatcgg
      121 ctgctgccag caatgcggcg gcaatcatca gggctttgat tttcatcatt ctttccttat
      181 cgtttgaatc ctgcctttga tggcgggttt ggggttgtcg gtggaatgcc gtcttgtgcg
      241 ggaacggtgc tcctgccggg tttccgacgg tcagggccgt ttgttgcgtc cggcggaaag
      301 agagtgggcg gggatgtagc cgggaacggc gacatccatc ctgtctgcgg ggtttctgcc
      361 gacactgctg aggtctttca actgaagtga gaaaaagaca gcgtttttgt aggtgttttc
      421 gccggtaacg tagcgttggg cgtacacgcc cgcgccccag cagccgcaac tgcttttgta
      481 ttccgcaccc gccagcacct ctatcggttt tttggcttca aaaccgtagt tgtaacggac
      541 gacggccgac aggttgcgcg tcagcggcca ttgtgcggac aggtcgagct ggctgagttt
      601 gtcgtaaaaa taggaaccgt cggacttcag gtagattttt tcgttgcgcc cgtatttgta
      661 gcgggcgttc agcactttgc cctgtgcggg acggtagctt gcaccgacgg cgtagttctc
      721 ggcgcgtttg tcgttttggt tgtagtggat gctgctgtcg aggatgaagc ggctgccgat
      781 gctgccggag gcaaatgcca cccagtcgga acggttgcgc ggttttttgc cgacgctgcc
      841 gtcaagcatc accgcatcat ccttgaaata gaatttctga ccgatgccgg cgcggaaacg
      901 ctcttccccc gtcgcgccgt ccaaaatacg gctttgcacg gcggcggaaa ggctgtttgc
      961 ggtgttaatc ctgtcgttgc cgtaatagag gttttcgcga aagagctgcc cgtagccgaa
     1021 gctgctttcc gacgaatcga aattgggcag gtcgttttgg gatttggcag gaatatagtt
     1081 gtagaacagg cgcggctcga gggtttgcag gactccgccg ccgaacatcc gcgtattccg
     1141 ctcaaaagtt gcgccgctgt cgatgttgac aatgggcaga gtgcggctga cgcgtcgggc
     1201 ttcttggctg ccgaagcggt tgaggctgta ataggtggcg tacagtccga gtttgggacg
     1261 gacgtaaccc cagctgttgc tgaaatccca tttgatgtcg ggatagacga cgaggcggct
     1321 gccgtcttgg cggctgtcgt ggctgaagcg ggtaaattgt gcggacacgc cgatttgcgc
     1381 cctgccggtg tttttgcgcc aatcggcgga aaggcgcggc atcagggcat acggtttgtc
     1441 tttgtagccg ctttggtttg ccagcgtctg gtatttcaga accgaaaggc cggcattcag
     1501 gctgccgccc gccgccctgc cgccataatc cagccataca cggcggttga ggttgacgtt
     1561 gccggcgatt tctttgttgc cgtaaaagtc gcggtagtag ccgctgtcgg agacttggtt
     1621 gaaatcgaca cccgcctgaa gcgtgtcgga aatgtcgtgc cggtgctgcc atttcgcctg
     1681 atagcggtta ttcctgccgc tttttttgtc gtgcggcagc caggtcaggt cggactggcc
     1741 ggcataatcc ggccgcaggt agcgtacctg cccgtcaaag accgcgccgc gttcgccgat
     1801 cacgccgggc gcgaacgtgg catcgagatt gggggcaagg ttgaaataat agggaacgga
     1861 aagggaaacg ccgtccgaac cggcggacag tgagggaaca agcaggccgc ttttgcggtt
     1921 gccgtcaagc gggaagtccg cccaaggcgt atagaaaagg ggaacgccgc cgaacacgaa
     1981 ggcggcgtgt ttggcaacgc ctatgccttt ttcccgatcg gcttcgacgg aagcggcctt
     2041 gacataccag ccggcatcgc cggcggaaca ggtgttgaat tgggtttccg tcagtttgta
     2101 atgcccttcg cccaacattt cggcggtgcg gctgacgctt tgcagccgcc gtccgccttg
     2161 ttcggtttcc atacggacgt tgtgcgcttc cccggtctgc tgctcgagat tgtaggtcag
     2221 ggtttcgccc cgaatcagcg taccgtcctg ttggagggcg aaccggtcgc ctgcggtaac
     2281 ggtgtcgccc gactggtcgt aatccgccca atcggtattg agggtcgtcc ggttgcgttc
     2341 gacgacgacg ttgccttcgg cacgcacctg cacctgcgac tgtccttcca ccttgtcggc
     2401 aacaatgcgc gtatagtctt cggggatgga tgcttcgccg ctgcctcgga cggcggcttc
     2461 ggttctctcg gggctgccgc tttcgttact gcaaaacagg caggtcgaac cgaggctcag
     2521 gctggtaggc tgtatgggtt cggacacgct ccgaacgctt cctccggcgg tcggattgtc
     2581 cgtttcctcc gccgcaacgg catcggcggc ggcgcaatgc gtgccgaagc agaggcccaa
     2641 tgccagcacc agtggtttga gtgaaaataa acgagccaaa atcgcccctc aagtcggttt
     2701 gccagttaga atagcgttta ttgtaacctg aaatgcttta gtactgttat gcaacgacaa
     2761 atcaaactga aaaattggct tcagaccgtt tatcccgaac gggacttcga tctgactttt
     2821 gcggcggcgg atgctgattt ccgccgctat ttccgtgcaa cgttttcaga cggcggcagc
     2881 gtcgtctgta tggatgcgcc gcccgacaag atgagtgtcg cgccttattt gaaggtgcag
     2941 aaactgtttg atatggtcaa tgtgccgcag gtattgcacg cggacacgga tttggggttt
     3001 gtggtattga acgacttggg caatacgacg tttttgaccg cgatgcttca ggaacagggc
     3061 gaaacggcgc acaaagccct gcttttggag gccatcggcg agttggtcga attgcagaag
     3121 gcaagccgtg aaggggtttt gcccgaatat gaccgtgaaa cgatgctgcg cgaaatcaac
     3181 ctgttcccgg aatggtttgt cgcaaaagaa ttggggcgcg aattaacatt caaacaacgc
     3241 caactttggc agcaaaccgt cgatacgctg ctgccgcccc tgttggcgca gcccaaagtc
     3301 tatgtgcacc gcgactttat cgtccgcaac ctgatgctga cgcgcggcag gccgggcgtt
     3361 ttagacttcc aagacgcgct ttacggcccg atttcctacg atttggtgtc gctgttgcgc
     3421 gatgccttta tcgaatggga agaagaattt gtcttggact tggttatccg ctactgggaa
     3481 aaggcgcggg ctgccggctt gcccgtcccc gaagcgtttg acgagtttta ccgctggttc
     3541 gaatggatgg gcgtgcagcg gcacttgaag gttgcaggca tcttcgcacg cctgtactac
     3601 cgcgacggca aagacaaata ccgtccggaa atcccgcgtt tcttaaacta tctgcgccgc
     3661 gtatcgcgcc gttatgccga actcgccccg ctctacgcgc tcttggtcga actggtcggc
     3721 gatgaagaac tggaaacggg ctttacgttt taaacccaat caaaatgccg tctgaaaacc
     3781 aagtttcaga cggcattttt caaacgggct tactgcgcgg ctttttgttc ttcgcgtact
     3841 ttgtccgcca aaaggtcgat ggtgtgcata ccggactccc agtcggcaaa ttcgacttta
     3901 tatttgccgc cgacgataac cgtgggcgta ccgtcgattt ggaaggtttc ggtcagctcc
     3961 tgcattttgt cggcgcgcgc ctggctttcg ggggactcgt aggcggcaag gacttttttg
     4021 ccgtcaaagg cggtttgttc gcccagccat tttttgagga cttccggctc ttgcagcttg
     4081 attttttggt tgaccatcgc atcgaaaata tggctgttcg ccacatcttt gctgtcggcg
     4141 gcagccatat cgacggcggc ggcgaggcgt gcgagcgtca gcatttcttt ctgccagacg
     4201 acgtgttcgg tacgcaggta catatcgtct ttaaaagact tggcgtgttt gcttaaaaca
     4261 ggttcgaggt gggcgcagtg cggacagaaa tagccgaaaa attcaaggac ttcgactttg
     4321 cctgcctgct gttggggaat cgggttggca aggacggtat agttttgccc ttcgaccagc
     4381 cctgccgggg cggcggctgc cgaagcggca ggcgcgctgt cggcggggac gctggtttgg
     4441 actttgctgt cgcacgcggc aagggcgaac agggcggcaa cgccgagggc gaggtgtctg
     4501 gatttcatac ggctctccgt gatgttggaa acagatgttg gaaatcaatc ggaatccggc
     4561 tattttattg catttccgcg tgttgataca gtttgccgcc ggaaaaggaa gctttcgttt
     4621 cgggaaaccg cttcagacgg catcaaaccc gatgccgtct gaagcggttt ctgtcgtaca
     4681 atacgcgccg ttgccccaaa cgggtacgac tgttgaggaa caatgatgaa tatgctggga
     4741 gctttggcaa aagtcggcag cctgacgatg gtgtcgcgcg ttttgggatt tgtgcgcgat
     4801 acggtcattg cgcgcgcatt cggcgcgggc atggcgacgg atgcgttttt tgtcgcgttc
     4861 aaactgccca acctgcttcg ccgcgtgttt gcggaggggg cgtttgccca agcgtttgtg
     4921 ccgattttgg cggaatataa ggaaacgcgt tctaaagagg cgacggaggc ttttatccgc
     4981 catgtggcgg ggatgctgtc gtttgtgctg gtcatcgtta ccgcgctggg catacttgct
     5041 gcgccttggg tgatttatgt ttccgcaccc ggttttgcca aagatgccga caaatttcag
     5101 ctctccatcg atttgctgcg gattacgttt ccttatatct tattgatttc actttcctct
     5161 tttgtcggct cggtactcaa ttcttatcat aagttcggca ttccggcgtt tacgcccacg
     5221 ttcctgaacg tgtcgtttat cgtattcgcg ctgtttttcg tgccgtattt cgatcctccc
     5281 gttaccgcgc tggcttgggc ggtctttgtc ggcggcattt tgcagctcgg cttccaactg
     5341 ccctggctgg cgaaactggg ttttttgaaa ctgcccaaac tgagtttcaa agatgcggcg
     5401 gtcaaccgcg tgatgaaaca gatggcgcct gcgattttgg gcgtgagcgt ggcgcagatt
     5461 tctttggtga tcaacacgat tttcgcgtct tatctgcaat cgggcagcgt ttcatggatg
     5521 tattacgccg accgcatgat ggaactgccc ggcggcgtgc tgggggcggc actcggtacg
     5581 attttgctgc cgactttgtc caaacactcg gcaaaccaag atacggaaca gttttccgcc
     5641 ctgctcgact ggggtttgcg cctgtgtatg ctgctgacgc tgccggcggc ggtcggactg
     5701 gcggtgttgt cgttcccgct ggtggcgacg ctgtttatgt accgcgaatt cacgctgttt
     5761 gacgcgcaga tgacgcaaca cgcgctgatt gcctattctt tcggtttaat cggtttgatc
     5821 atgattaaag tgttggcacc cggcttctat gcgcggcaaa acatcaaaac gcccgtcaaa
     5881 atcgccatct tcacgctcat ctgcacgcag ttgatgaacc ttgcctttat cggcccactg
     5941 aaacacgtcg gactttcgct tgccatcggt ctgggcgcgt gtatcaatgc cggattgttg
     6001 ttttacctgt tgcgcagaca cggtatttac caacctggca agggttgggc ggcgttctta
     6061 gcaaaaatgc tgctctcgct cgccgtgatg ggaggcggcc tgtatgccgc ccaaatctgg
     6121 ctgccgttcg actgggcaca cgccggtgga atgcaaaagg ccgcccggct cttcatcctg
     6181 attgccgtcg gcggcggact gtatttcgca tctctggcgg ctttgggctt ccgtccgcgc
     6241 catttcaaac gggtggaaag ctgaccgatg ccgatatcct ttttcggaaa acgctttttc
     6301 cgcgcctata ttcgggcgcg catcccccgc gtccgcacca gccggacggg ctgccgcatc
     6361 atcaatgccg tctgaaaaca gaaaaaccga taccttatga ttttgacacc gccggacaca
     6421 ccgtttttcc tccgcaacgg caatgccgat acgattgccg ccaaattcct gcaacgcccc
     6481 gcacccgcat accgccgcga gctgcttccc gacagcacgg gtaaaaccaa agtcgcctac
     6541 gacttttcag acggcatttc gcccgatgcg ccgctggtcg tgctgtttca cggtttagaa
     6601 ggaagcagcc gcagccatta cgcggtcgaa ctgatgctcg cggtacgcag ccgcggttgg
     6661 cacggcgcag tcgtccattt ccgcagctgc ggcggcattg ccaacaccgc tccggtgttc
     6721 taccacttgg gcgataccgc cgaaatcgcc tttactttgg acacgcttgc cgcgcgttac
     6781 cgtgaaatat acgccgtcgg cgtatcgctg ggcggcaacg cgctggcaaa atatttgggc
     6841 gaacagggcg aaaacgcgct gccgcaagcc gccgccgtca tctccgcacc cgtcgatgca
     6901 gaggcggcag gcaaccgctt cgacagcggc atcacacggc tgctctacac gcgctacttc
     6961 ctccgcacac tgatacccaa agcacggtcg ctccaaggtt ttcagacggc atttgccgca
     7021 gggtgcaaaa cactgggcga gtttgacgac cgcttcaccg caccgctgca cggctttgcc
     7081 gaccggcacg actactaccg ccaaacttcc tgcaaaccgc tgctcaaaca cgttgccaaa
     7141 ccgctgctcc tgctcaatgc cgccaacgac cccttcctgc cgcccgaagc gctgccgaca
     7201 ccggacgaag tgtccgaagc cgttaccctg ttccagccgg cacacggtgg tcatgtcggc
     7261 tttgtcagca gtaccggcgg caggctgcac ctgcaatggc tgccgcagac cgtcctgtcc
     7321 tatttcgaca gcttccgcac aaacaggcgt taacggtttg atgctaatat tccccctttc
     7381 cccggacgaa tacggaacac aatatgaccg acatcctcaa taaaatcctt accgccaagg
     7441 cacaggaagt tgccgcccaa aaagccgccg ttcccctcga acacatccgc gcacttgccg
     7501 cagaagccgc gcccgtccga agcttcatcg gttcgatacg cggcaaacac cgcctaaacc
     7561 tgcccgccgt cattgccgaa atcaaaaagg caagcccgag caaaggcctg atccgtccgg
     7621 acttccgacc ggccgagatt gcacgcgcct atgaaaacgc cggagcggcg tgtttgtccg
     7681 tactgaccga cgaaccctat ttccaaggtt cgcccgaata cctcaaacag gcgcgcgaag
     7741 ccgtatcgct gcccgtcttg cgtaaagact tcatcatcga cgaatatcag gtttatcagg
     7801 cgcgcgcctg gggggcggat gccgtcctgc tgattgccgc cgcactggaa cgggaacaat
     7861 tggaacgctt tgaagcggtg gcgcacgaat tgggtatgac cgtcctgctc gagctgcacg
     7921 atgaaagcga attggaaaaa tgccgtaacc tgaccacgcc gttgtggggc gtaaacaacc
     7981 gcaacctgag gacttttgaa gtctccctcg accaaaccct gtcgctgctg cccgcactgg
     8041 aaggcaaaac cgtcgttacc gaaagcggca ttacgggtaa ggcggatgtg gaatttatgc
     8101 gggcgcgcgg cgtgcacacc ttcctgatcg gcgaaacgtt catgcgtgcc gacgatattg
     8161 aggcggaagt gggcaaactc ttctaaatcc cgatttcaga cggcatattg ccgccaaccg
     8221 accaacaccc ttaccgagca cggaaccatc atgcaccgac cgaccgccaa acagattctg
     8281 cacgaagtat ttggttatcc cgaatttcgc ggcaggcagg aggctgtcat caatactttg
     8341 gcaggcggcg ggagtttgac ggtgctgatg ccgacgggcg gcggcaagtc tttgtgttac
     8401 cagattcccg cgctgatgtg cgaaggcgtg gcggttgtcg tatcgccgct gattgcgttg
     8461 atgaacgacc aagtggcaaa cctgcacgcc gccggcatcg aagcggcggc agtcaacagc
     8521 ggcacatcgg cagatggggc gcgcgagatt gccgacaagc ttgcccaagg ccgtctgaaa
     8581 ctgctttatg tcgcaccgga acgcctagtt accgaccgct ttttgcgttt tctcgaccaa
     8641 caaaccgtca gcctgttcgc cattgacgaa gcgcactgcg tcagccagtg gggacacgat
     8701 ttccgccccg aataccaaca gctcggcatc cttgccgaac gctatccgaa cgtcccgcgc
     8761 atcgccctga ccgctaccgc cgatgccgcc acgcgcgccg acatcaagca ttatctgcac
     8821 ttggacaatg cgcccgaatt tgtctccagc ttcgaccgtc cgaatattta ttatcaggtt
     8881 atcgaaaaaa acaacggcaa aaaacaattg ctggatttca tccgcaaaga aatgacgggg
     8941 caaagcggca ttgtgtattg cctaagccgc aaaaaggttg aagatgtggc gcagtttttg
     9001 cgtgaaaacg gattaaacgc gattccgtat catgctggtt tgagcatgga cgtgcgcgag
     9061 gaaaaccaac gccgctttac gcatgaagac aatattatcg tggtggcgac cgtggcgttc
     9121 ggcatgggca tagacaaacc cgacgtgcgc tttgtcgccc atctcgatat gccccagagt
     9181 gtcgaacatt tctatcagga atcggggcgt gccggccggg acgggctgcc tgccgcgagc
     9241 tggctgtgtt acggtttgaa cgattgggtg ttgctgcgcg aacggattgc cgaaggcaac
     9301 agcgacgagg tgcaaaagca aatcgaaatg caaaaactcg atgccatgct ttccgtctgc
     9361 gaaaccgccg cctgccgccg cgtactgctg ctcaaacatt tcggcgaaga atccgaaccc
     9421 tgcggccatt gcgacaactg cctgcatccg cccgtacggt ttgacggcac ggtgttggtg
     9481 caaaaattac tcagctgcgt gtaccgcgcc ggacaacgtt ttgccgccgg ttacatcacc
     9541 aaccttttgc gcggcaaaag cgacgattgg atacgcggca accggcacga acaactgtcc
     9601 accttcggca tcggtacgga gttgtccgac aaagaatggc gcagcgtcat ccgccagtgt
     9661 atcagcctcg gctacctcac cgtcaacatt acccgatatc aggcattgca actgaccgaa
     9721 gccgccaaaa aagtcctcaa aggcgaaacc gaagtgatgc tgcgtccgct caagcgcgac
     9781 aagcccgccg cccgcaccct caaagacaac tggctgcgta ccgaacgcga agaacgcctg
     9841 tggcaggcat tgcgcgtttg gcgtatgaaa caggcagaag ccgaagtcat ccccgcctat
     9901 atgattttcg gcgacaaaac cctgcgcgac cttgtcgaaa aaatgccgca agacctcaac
     9961 ggactgcacg acatctacgg tttgggcgaa gccaaaatcg accgtttcgg acacggcatc
    10021 ctcgaagtct gccgcaacac cgccggcttc agtcgcgacg cggtcatccg tccgcaaacc
    10081 gaacgcgaac aacaactgcg tcaaaaactc gaggcctggc ggtatgaaca ggcaagggcg
    10141 gaaaactgcg ccctgcatgc cgtcctctcc gacgaaagcc ttgccgatat gcttgccgcc
    10201 acgcccgaaa ccgaaaccga cctcgaaggc gtacacggct tgggcagcgt acgcgccgcc
    10261 aaatacggac gggacatcct cgccgtctgc cgtccgtttt cagacggcat cgatgaagcc
    10321 gccaaacgca aacgccgcct gatgcgcgcc ctgatccaat ggtgcggcga aacggcaaaa
    10381 cacgaacagt ccgaacccta ccgcattctc agcaaagccg ccctgcgcgc cattgccgcc
    10441 aaacagccga agggtttggc ggagcttgcc gccgtatacg gcgtgggcga agaaaaaacc
    10501 gcacgttacg gtgcggcggt gttggcggtg ttgggacaaa atgccgtctg aaaaccgtta
    10561 ccaagtttca gacggcattg cctctattta aaaattcct
//
LOCUS       DAXHTR010000070        10490 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.070, whole genome shotgun sequence.
ACCESSION   DAXHTR010000070 DAXHTR010000000
VERSION     DAXHTR010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10490
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.070"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(61..828)
                     /locus_tag="ACFNML_001228"
     CDS             complement(61..828)
                     /locus_tag="ACFNML_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HFC6392614.1"
                     /translation="MSSNKASFFTRLRRLCRLAVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGVLAALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
     gene            complement(916..1743)
                     /gene="mutM"
                     /locus_tag="ACFNML_001229"
     CDS             complement(916..1743)
                     /gene="mutM"
                     /locus_tag="ACFNML_001229"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HFC6392615.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCADYLYARLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPYRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFVDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            complement(1795..2460)
                     /locus_tag="ACFNML_001230"
     CDS             complement(1795..2460)
                     /locus_tag="ACFNML_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFC6392616.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPCGRL
                     MLTGFNPYSLWGFSRWFDGERLPEKRFCLPLPELKRRLADAGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYALVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            2639..4615
                     /locus_tag="ACFNML_001231"
     CDS             2639..4615
                     /locus_tag="ACFNML_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HFC6392617.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASRSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIIPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRARLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            4683..5375
                     /locus_tag="ACFNML_001232"
     CDS             4683..5375
                     /locus_tag="ACFNML_001232"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase"
                     /protein_id="HFC6392618.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYILLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLESPSALLLIITEGKYHQVKRMVAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIDCPKL"
     gene            complement(5576..5682)
                     /locus_tag="ACFNML_001233"
                     /pseudo
     CDS             complement(5576..5682)
                     /locus_tag="ACFNML_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6005..7320
                     /locus_tag="ACFNML_001234"
                     /pseudo
     CDS             6005..7320
                     /locus_tag="ACFNML_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519747.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            7564..8220
                     /gene="cmk"
                     /locus_tag="ACFNML_001235"
     CDS             7564..8220
                     /gene="cmk"
                     /locus_tag="ACFNML_001235"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFC6392619.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSKLAKKLPAVFSGSRILLDGEDVSDGIRTEAIGMGASAVAQLP
                     KVRASLLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            8375..10060
                     /gene="rpsA"
                     /locus_tag="ACFNML_001236"
     CDS             8375..10060
                     /gene="rpsA"
                     /locus_tag="ACFNML_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFC6392620.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVVALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            10071..10385
                     /locus_tag="ACFNML_001237"
     CDS             10071..10385
                     /locus_tag="ACFNML_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFC6392621.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
BASE COUNT         2743 a         3083 c         2359 g         2305 t
ORIGIN      
        1 aatttaatcc actatacatc cacaaaagcg aatcatactg ccacattccg acaaccggtt
       61 tcagacagcg atatccgcat cgtcggcgac aacggcacgg atgctttctt cgattttatc
      121 ttttaaagca taacggtctt cgctttccgc cgcatccgcc acgcaaacga aatcgacttt
      181 tatcgtcaat tttttcatag acacgatgcg ccacaggcag gtcggcaaac cgacatcggc
      241 atatgaggga cgagccgtcc tttttcccgt ttcgtcataa taacgcagcg cgaccgccaa
      301 aacctttgcc cccgcatcga tggcggattg gaacagcgcg gctttgaacg gcaaaagccc
      361 caatccggag gaagtccgcg cttcggggaa aaaactgacg ttttgaccgc gttgcaaggt
      421 ttcgcagacg gcgcggttaa tcggttcgat gtcgcgccgc gaattgcggt tgatgaacac
      481 cgttcccgcg ttctgcccca tcttgcccaa taccggccag cttttgattt cctgcttggc
      541 gataaagctg ctcggataaa ccgcgctcat cgcgaaaata tccagccagg acacgtggtt
      601 ggcggcaacc aagacaccgt tcggatgttc gggtgcgggt ctgcccacct ccaatccgat
      661 atccaaagcc gccaaaaccc ccctgcccaa ctcgattacc gcccgattgc gcgactcggg
      721 gcaaccgccg tcaataccgc gcaggttttt cccggttttg aacagccaga ccgccaaacg
      781 gcacaagcgg cgcagacgtg taaaaaatga agctttattt gaagacatga gcaattcttt
      841 cccgataatc ggtgtttgtt gttgaaacgt tcggacggca taccgataaa atgccgtctg
      901 aaaccgtttt cagtcctatt tctgacagtt cgggcaataa aacgtgccgc gctgccccaa
      961 agtttctttc acaaccaaac cgccgcactg ggggcacggc tgattgtgcc gcccgtacac
     1021 cgtatattcc tgttggaagt agccgctttt gccgtcgctg tccacaaaat ccctcaaggt
     1081 actgccgccc gtttcaatgg cgcgccgcaa caccgctttg acggtttcaa ccaaaagcgc
     1141 gcactctttc tttttcaggc ggttggcagg acggtagggc gaaatgcccg ccctaaacag
     1201 gctctcgttg gcataaatgt tgcccacacc gaccacgacc gcattgtcca tcagggcaag
     1261 tttgaccgcg cgcttctgcg ccttcagcct tgcatacaga taatccgcac aaaatgcctc
     1321 cgacaaaggc tccggcccca gtttttccaa cagcggatga tgttcttcga ttccctcata
     1381 ccaaagtatc gcgccgaact ttctcggatc gcggtaacgc atgaccgtgc cgtctgaaaa
     1441 cacaatatcg acgtgatcgt gtctgtccgg cctgccgata cgtccgtccg acggcgtaaa
     1501 aatccgcaag ctgcccgaca tccccaagtg aatcagcagc acgcccgttt gaaagcggat
     1561 aagcaggtat ttcgccctcc tgccgcagga caacacctgc cggccggaca aaatctcccc
     1621 caaatcggga ttaatctgcc agcgcagctt caattggcgc aataccacgg cttccaccgt
     1681 tttcccttca atatgcggcg cgatgccgcg caacgtcgtt tccacttccg gcaattcagg
     1741 cataacccct cccgacattt tttctgacag atgccgtctg aaagacggcc gtccctaatc
     1801 cgcaaccctt gccgcacccg ccgcaagggc tttgccgccc aaatacccgt cccacgcggg
     1861 caggggcgtt acgcccgccg cccttttgac caaaaccaaa gcgtacactg cggcacattg
     1921 cggccaccaa cggtcgcccg ccttttccat aaaccgccaa aagcgtattt gcccgagcga
     1981 cgaaaccggc ggcagataca ccataaattt cccaaattca atatcgaaac cggcatcggc
     2041 aagccgcctt ttcaactcgg gcagcggcag acaaaaccgt ttttccggca ggcgttcgcc
     2101 gtcaaaccaa cggctgaatc cccagagcga atacggattg aaacccgtca gcatcaagcg
     2161 tccgcacggt ttcaatatcc ggtgcgcttc cgacaggatt tgcgaaggaa caccgccttc
     2221 aagcgtatgc ggaaaaagca gcatatccgc agaaacatcc gccaaagcca tattctccgc
     2281 cgacatcgac atatctcgcg gcacacagac aacatcttca gacaggctca gccacctgcc
     2341 gcccacctga accgcacaca ttcccgaaaa acggtatgaa tccagatacc gcccgaagaa
     2401 atcctgttcc aattttgcaa cataccgccc catcgccgta tcttcaaacc atgcatccat
     2461 attgcgtccg tttcaaacag attgcctgcc gatattctat tccaaacagg atttctgtca
     2521 aaaaacacat cggccgccca tttccgaatc cgcataaagt tcctgtaaaa cttgacgctt
     2581 tttcagtcaa acagtaccat cggacgataa aatatgttta ttccgccgaa tataaatcat
     2641 gtccaaactc aaaaccatcg ctctgaccgc atcaggtctg tccgtttgtc cgggtttcct
     2701 atacgcccaa aacacctcat cacaccaaat cggtttggcg attatgcgct taaactcttc
     2761 aatactcgac ctgcccccga caaaacaata tttccaatcc ggcagcctgt ggagcgagct
     2821 gcgccaaggc ttccggatgg gcgaagtcaa tcccgaactg gtacgccgcc acgaaagcaa
     2881 attcatcgca agccgcagct atttcaacag ggtcatcaac cggagtagac cctatatgta
     2941 ccatatcgcc aacgaagtca aaaaacgcaa tatgcccgcc gaagccgccc tgcttccctt
     3001 catcgaaagc gcgttcgtca ccaaagccaa atcacacgtc ggcgcatcag gattatggca
     3061 gtttatgccc gctaccggca ggcattacgg cctggaaaaa acaccggttt acgacggcag
     3121 gcacgacgtt tacgccgcca ccgatgccgc actcaactat ctgcaatacc tctatggact
     3181 gttcggcgac tggccgcttg cctttgccgc ctacaactgg ggtgaaggca acgtcggacg
     3241 cgccatcaac cgcgcccgcg cccaagggct cgaaccgacc tacgaaaacc tgcgtatgcc
     3301 caacgaaacg cgcaactatg tccccaagct gctcgccgtg cgcaacatta ttgccactcc
     3361 ccaatctttc ggcatgaata tcagcgacat agacaacaaa ccctattttc aggcagtcga
     3421 accggatcgt ccgctcgaca acgaagccat cgcccggctt gccggcatca cgcaaagcga
     3481 gctgctcgcc ctaaaccccg cattcaacgt ccccgcgttt atccccaaaa gcaaacgcaa
     3541 actgctgctt cctgtcgcgt ccgtacaaac cttccaaagc aactacctca acgccgcacc
     3601 cgacagcctg ttttcatggg aagtctatac gcctgccgcc aaaaccagcc tgtccgacat
     3661 ctcgacggca accggcatga gcattgccga catcaaacgc ctcaacaacc tgaacggcaa
     3721 ccttgtcaac gcaggacgca gcatccttgt cgccaagaac ggcaaaaccc ttcagacggc
     3781 atcggaatcc gtcgtttcca tcgacatcga caatacaccc gacacctacc gttccaatat
     3841 gccggcaggc acggtgaacg tcggcattgc ccgaatccga cccgccgccg cacagacagc
     3901 ggacattacc gtcgcacctt tgccgcagaa aaccgtccgt acggaacccg atccccttgt
     3961 ccgtattgcc gaacctgccc ttgcgacagc cgcagcgcaa cctcaaaccg aaaaacagac
     4021 cgccatgccg tctgaaaccc aaaccgcaac actcgcgcag atcatccccc aaaacgacat
     4081 gcaggcggca gacgaactca tgcagcttgt tgcccgaaac aacctgcgcc gacaggctga
     4141 agaaaccatc tccgccgtca tcggcacgcc tgacacagtt gccgaacaca acatttcctc
     4201 atctccgcaa cataccgttg ccgccgacgg caaacgccgg gcacgtttgg aaacgcgcgt
     4261 agccaaagct gccgacggcg aagccgaaat ctccccgctc catgccagca tccaccgcgt
     4321 tgtagaaggc gacaccctgt tcaacattgc caaacgctac aacgtcagcg tagccgacct
     4381 gattgtcgcc aacaacatca aaggcaacac catccaaaaa ggacaggtac tccgcctggc
     4441 gcaggcagcc cctgcccaaa cccgtattga aaaagtatcc tacaccgcgc gcaaaggcga
     4501 caccttcaaa agtatcgccg cgcgcttcaa tatccatatc gacgacatcc gccggctcaa
     4561 tcccaacctg aacaccatca atccgggaca gagggtcaaa ctgattggaa gctgattcgg
     4621 atacggcaca tgacaggact ttctcagtcc tgtctttttc atcccacatt tcccatacca
     4681 tcatgaaact catcaaatac ctgcaatatc aaggcatagg aagccgcaag cagtgccaat
     4741 ggctgattgc cggcggttat gttttcatca acggaacctg tatggacgac accgatgcag
     4801 acatcgattc ctcatctgtc gaaacgttgg atattgacgg ggaagcagta accgtcgttc
     4861 ccgaacccta tttctacatc ctgctcaaca agcccgaaga ttacgaaact tcgcacaaac
     4921 cgaaacacta ccgcagcgta ttcagcctgt tccccgacaa tatgcggaac atcgatatgc
     4981 aggcggtcgg caggctggat gcggatacga ccggcgtatt gctgattacc aacgacggca
     5041 aactgaacca caacctgact tcgccgagca gaaaaattcc caagctatac gaagtaacgc
     5101 tcaaacaccc cacaggagaa acgctctgcg aaaccctgaa aaacggtgtc ctgctccacg
     5161 acgaaaacga aacggtttgc gccgccgatg ccgtgttgga aagcccgtcc gcattgctcc
     5221 tgatcattac cgaaggcaaa taccaccaag tcaaacggat ggtcgccgcc gcaggcaacc
     5281 gcgtgcagca tcttcaccgc cggcgattcg cacatttgga aacagaaaac ctgaaacccg
     5341 gcgaatggaa atttatcgat tgtccgaaac tctaaaataa catccaagaa ttccatttat
     5401 attcatccac atactcatta aatatatggt ttaacccaat ttaacgcaaa aaatcaattt
     5461 acgatataat ccatttgtct gagtaacgcc tgttcaagca ggcttatgag taagacgttt
     5521 tccccgtaat gtgttttgcc atctatactt ctccccttgt atagatggtt ttttatagtg
     5581 gattaaattt aaaccagtac agccttgcct cgccttgccg tactatttgt actgtctgcg
     5641 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttcaaattcc ctctctttca
     5701 tcattattta aatgctatca ttaatataat taacaaaatt tcatttccat acaccgatgt
     5761 agaagaataa caaattaatc tatttataaa ctccgattct tcctcatcgg gttatactca
     5821 tcagaaatac tcactgaaat atatgccttt tacatggaaa atcactagac agttgaaaaa
     5881 caattagtca gttcctgtta aaaaacgcgt gtagaatttc cgctttatct caatcggaca
     5941 cggtttcaat atgtcttccc cttcaaatac caatcgtcaa acctgatcca gccgattaac
     6001 ctatatcctg accgttgccg gcgcgactgt cggcttcggc gcgacgtggc gttttcccta
     6061 ccttgtcggc gaaaacggcg gcggcgcgta tgtgttttta ttctgtatcg cgatgctggt
     6121 tatcggcatc ccgatgattt tggtggaaaa tgtcatcgga cggcgcaaag gcgtgaacgc
     6181 gctggatgcg ttcggcggcc cgatgaacgg caaacccatt gccaaaattt ggaaactggt
     6241 cggctggatg ggctgctcgg cgcgttcggc atcatggctt attacatggt gctcggcggc
     6301 tgggttatca gctacattgt taatatcatc ggcggtaatt tggacatttc cagtcctgtt
     6361 tccagcgaag tcaccaaaag tttctttacc gaacacatcg aaaacagtcc ttgggaaatt
     6421 gctttctaca ccttcctatt tgtggttgtg aaccaatgga tcttggtaaa aggcgtgatc
     6481 ggcggtattg aaaaagcggc aaaatacctg atgcctttgc tgtttttatt cctgattgcc
     6541 atggttgtgc gcaacgttac cctgccgggc gcaatggaag gcattacctt ctatctgaaa
     6601 cctgacttca gcaaaattac agccgaactg ttcgtcttcg ttttaggaca agtgttcttt
     6661 accttgagct tgggtttcgg cgtgatgatt accttgtcca gctatctgga taagaacgaa
     6721 aatctggtgc aaaccgcggt catcacggcc attaccaata ccatcatcgc cgtactggcc
     6781 ggctttatga ttttcccgtc gctcttcagc ttcggcgtcg cgcccaattc tggcccgact
     6841 ttggtattcc aaagtttgcc gattgtgttc tcacatatgt gggcaggacc tgtgttcgct
     6901 gtcattttct tctctctgct cctgattgcc gcgctgacaa cttcgctgac gatttatgaa
     6961 gtgttgatta cgaccattca ggaaaaaacc aaaatccgcc gtaccgccgc aatcacgatt
     7021 gtattgtccg ccatcttcat cttcggcaat attccgtcca ttctgagcta tggcccgtgg
     7081 aaagatgttt ccatatttgg caaaaatatt tttgacgctt ttgactacat cagcggcaac
     7141 atcttgttta tgctgaccgc gctcggctcc gcgctgtttg tcggttttgt gatgaaggat
     7201 gaagcgaagg acgaattgct ttataaaggc aaccatataa cggtcaatat ttggtttgcc
     7261 tatgtgaaat accttgtgcc gctggtgatt ttgctgattt tcgtcagcaa cctgttctaa
     7321 tccgcagcaa tcgatgccgt ctgaaagtca tacctctttc agacggcatt gcgttgatgc
     7381 ccgccacaat acccgcaacg ccagaatccg aaccggcttg ccggcttctg tcggatattt
     7441 ccgggcaggc ggcgttccgt ctgcttagac aatcgtcctt taaaacaggt agaatctgcc
     7501 ccaacgggaa acacatcctt cagacggcaa aacccatacc ccaaaccatc aggaatcccc
     7561 cttatgaaca gacaaaaagt catcgccatc gacggcccgg gcgcatcggg caaaggcacg
     7621 gttgccgccc gcgttgccgc cgcattggga tacgattatc tcgataccgg cgcactctac
     7681 cgcctgaccg ccctatatgc acaaaaacaa ggcgtggaat ggcacgatga agaaaacgtt
     7741 tccaaactgg caaaaaaact gcccgccgta ttttcaggca gccgcatcct gctcgacggc
     7801 gaagacgttt cagacggcat ccggacagaa gccatcggca tgggcgcatc cgcagtcgca
     7861 cagttgccta aagtccgcgc ctccctgctg caacgccaac gcgattttct gaccgaaaaa
     7921 ggactggtcg ccgacggacg ggacaccgga tcggtcgtct tcccccaggc cgaactcaaa
     7981 atttttctga cagccgaatc caaaatcagg gccgaacgcc gcgccaaaca aatcggcatc
     8041 ccctgcgaag gtttggcatt cgagcgcatc ctgtccgaca tcgaagccag agacgaggca
     8101 gaccgaaacc gcaaggttgc ccccctgaaa caacaacccg atgccctgct tttggatacg
     8161 agccgcctga ctatagaaga aactgtaaaa aaagtgcttg attggtatcg taaagtttaa
     8221 attttcaggt ataatcgccc gcattgcgtt tcagacagca tgaatttccc catatgccgt
     8281 ctgaaacctt atttacccgt ctgccctgcc aaggacggca gacacccacc aacccacccc
     8341 gcaccccttg gcggtgtacc gaaaagagtt atatatgtct atggaaaatt ttgctcagct
     8401 gttggaagaa agctttaccc tgcaagaaat gaacccgggt gaggtgatta ccgctgaagt
     8461 agtggcaatc gaccaaaact tcgttaccgt aaacgcaggt ctgaaatcag aatccctgat
     8521 tgatgtagct gaattcaaaa acgctcaagg cgaaattgaa gttaaagtcg gcgacttcgt
     8581 taccgttacc atcgaatccg tcgaaaacgg cttcggcgaa accaaactgt cccgcgaaaa
     8641 agccaaacgc gctgccgatt ggattgccct ggaagaagcc atggaaaacg gcaacatcct
     8701 gtccggcatc atcaacggca aagtcaaagg cggcctgacc gttatgatta gcagcatccg
     8761 cgcattcctg ccgggttctt tggtcgacgt acgtcctgta aaagacactt ctcacttcga
     8821 aggcaaagag atcgaattca aagtgatcaa actggacaaa aaacgcaaca acgtcgttgt
     8881 ttcccgccgc gccgttctgg aagccacttt gggtgaagaa cgcaaagccc tgctggaaaa
     8941 cctgcaagaa ggctccgtca tcaaaggcat cgttaaaaac attaccgatt acggtgcatt
     9001 cgttgacttg ggcggcatcg acggtctgtt gcacatcacc gatttggcat ggcggcgcgt
     9061 gaaacacccg agtgaagtct tggaagtcgg tcaggaagtt gaagccaaag tattgaaatt
     9121 cgaccaagaa aaacaacgcg tttccttggg tatgaaacaa ctgggcgaag atccttggag
     9181 cggtctgacc cgccgttatc ctcaaggcac ccgcctgttc ggcaaagtat ccaacctgac
     9241 cgactacggc gcattcgtcg aaatcgaaca aggcatcgaa ggtttggtac acgtctccga
     9301 aatggactgg accaacaaaa acgtacaccc gagcaaagtc gtacaactgg gcgacgaagt
     9361 cgaagtcatg attttggaaa tcgacgaagg ccgccgccgt atctctttgg gtatgaaaca
     9421 atgccaagcc aatccttggg aagaatttgc cgccaaccac aacaaaggcg acaaaatctc
     9481 cggcgcggtt aaatccatta ccgatttcgg cgtattcgtc ggcctgcccg gcggcatcga
     9541 cggtttggtt cacctgtccg acctgtcctg gaccgaatcc ggcgaagaag ccgtacgcaa
     9601 atacaaaaaa ggcgaagaag tcgaagccgt cgtattggca atcgacgtgg aaaaagaacg
     9661 catctccttg ggtatcaaac aactggaagg cgatccgttc ggcaacttca tcagcgtgaa
     9721 cgacaaaggt tctttggtta aaggttccgt gaaatctgtc gatgcgaaag gcgccgttgt
     9781 cgccctgtct gacgaagtag aaggctacct gcctgcttcc gaatttgcag ccgaccgcgt
     9841 tgaagatttg accaccaaac tgaaagaagg cgacgaagtt gaagccgtca tcgttaccgt
     9901 tgaccgcaaa aaccgcagca tcaaactttc cgttaaagcc aaagatgcca aagaaagccg
     9961 cgaagcactg aactccgtca atgccgccgc caatgcgaat gccggcacca ccagcttggg
    10021 cgacctgctg aaagccaaac tctccggcga acaagaataa ggttgcagac atgacaaagt
    10081 ctgagttaat ggttcgtttg gcagaagtgt ttgccgccaa aaacggcacg catcttctgg
    10141 caaaagacgt agagtacagc gtaaaagtct tggttgacac catgactaga tcgcttgccc
    10201 gaggtcaacg catcgaaatc cgcggtttcg gcagcttcga tttgaaccat cgtcctgccc
    10261 gcatcggtcg caatcccaaa accggcgagc gtgtggaagt acctgaaaaa catgtacccc
    10321 acttcaagcc cggtaaagaa ttgcgcgagc gggtcgactt ggctttaaaa gaaaatgcca
    10381 attaaacctt agcatcaaaa cgccgctgtt acgcggcgtt ttttctgtgg tttaacttca
    10441 tccgctgctt caataccttg agccaagcaa gcaacggatt agagcgtgga
//
LOCUS       DAXHTR010000071        10431 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.071, whole genome shotgun sequence.
ACCESSION   DAXHTR010000071 DAXHTR010000000
VERSION     DAXHTR010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10431
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.071"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            176..1360
                     /gene="coaBC"
                     /locus_tag="ACFNML_001238"
     CDS             176..1360
                     /gene="coaBC"
                     /locus_tag="ACFNML_001238"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFC6392622.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTSLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILRGKKILITAGATFEAIDPVRGITNTSSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAEDMHRAVHRLIEKQDAFISVAAVSD
                     YKVKNKSTQKFKKDKNAKPLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAGYL
                     NK"
     gene            complement(1427..3583)
                     /locus_tag="ACFNML_001239"
     CDS             complement(1427..3583)
                     /locus_tag="ACFNML_001239"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6392623.1"
                     /translation="MPVPQPTAPYDPLTAEARALLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAAVF
                     GNTVAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGENLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGKHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDTCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3676..3882)
                     /gene="rpoZ"
                     /locus_tag="ACFNML_001240"
     CDS             complement(3676..3882)
                     /gene="rpoZ"
                     /locus_tag="ACFNML_001240"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFC6392624.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDVRNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3941..4558)
                     /gene="gmk"
                     /locus_tag="ACFNML_001241"
     CDS             complement(3941..4558)
                     /gene="gmk"
                     /locus_tag="ACFNML_001241"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFC6392625.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRNALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4686..5285
                     /locus_tag="ACFNML_001242"
     CDS             4686..5285
                     /locus_tag="ACFNML_001242"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFC6392626.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5331..6119)
                     /locus_tag="ACFNML_001243"
     CDS             complement(5331..6119)
                     /locus_tag="ACFNML_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HFC6392627.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALQHIASDFFADKDYFSSKPVYQMLVFAEENEMPP
                     WSDIVEREGLKTVRWHEEAVDLLPAGMSKTDGIRSVVGALGLEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6444..7799
                     /gene="yegQ"
                     /locus_tag="ACFNML_001244"
     CDS             6444..7799
                     /gene="yegQ"
                     /locus_tag="ACFNML_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFC6392628.1"
                     /translation="MKAPELLLPAGGLERMRAAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQQCPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRSIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLTGHSIAKQSQYVGHVTEIDAEGWATVEVKNR
                     FAIGDSLEIIHPSGNQTIKLEQMARKGQPVDVAPGNGIQVKIPNMRGKEKALIARVLN
                     P"
     gene            7933..8409
                     /locus_tag="ACFNML_001245"
     CDS             7933..8409
                     /locus_tag="ACFNML_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HFC6392629.1"
                     /translation="MLAACQSGSAPKNAHTASPGTPAGESCKVIRGDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPVANGRNLVTRQAANGIGKSDSEAYERA
                     LINAARKFQQTAGKLGGRGVTGFHSYFDKQPLQGGQYDCQAGSFHVRVVMRANVVR"
     gene            complement(8739..8924)
                     /locus_tag="ACFNML_001246"
                     /pseudo
     CDS             complement(8739..8924)
                     /locus_tag="ACFNML_001246"
                     /inference="COORDINATES: protein motif:HMM:NF013466.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
     gene            complement(8940..9548)
                     /locus_tag="ACFNML_001247"
     CDS             complement(8940..9548)
                     /locus_tag="ACFNML_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6392630.1"
                     /translation="MSNSSFKLSALAIACGLALSACSSGGGDAPSVKPTPTVPGASNP
                     APANPEPSAPAASPLKTGELGEKYFYGEGEITKLVINGETINLTVADEQLSRRVKLWT
                     DFDKIITSQGFENLIYGAAKLADGSYALFVQGKLSDSLPGDTVTYSGDVLHFRSRNLT
                     EGNWFNEGNSFRAAGSFTATVNFAGKSLNGKINSGDGLYMGT"
     gene            complement(9742..10371)
                     /locus_tag="ACFNML_001248"
     CDS             complement(9742..10371)
                     /locus_tag="ACFNML_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HFC6392631.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLKDLGEEPYKFDKKLPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
BASE COUNT         2617 a         2834 c         2463 g         2517 t
ORIGIN      
        1 ctgcgccgcc tccaacaatc ctttcctccc cctcctctga ctggtgcgcc tttgtgaata
       61 tgccgtctga aactcggggc ttcagacggc attttgtatc caaacggtat ctaatgtatc
      121 cacactttgt tatagaatgg ctgctgtttt ttcttcgtaa ttagaaattg tcaaaatggg
      181 caaacatatt cttttaggtg taacgggcag tattgcggcg tataagtctt gcgagttggt
      241 tcgtctgctg aaaaaacagg ggcattcggt tacggtggtt atgagccgct cggcaactga
      301 atttgtttct ccgctgactt ttcaggcttt aagcggcaat cctgtcctga ccgacacgca
      361 cggcggcaac ggttcaaacg gtatggaaca tatcaacctg acccggaatg cggatgtttt
      421 tctgattgcg ccggcaagta tgaataccgt ggcaaaaatc tgtaacggcg tggcagataa
      481 cctactgacc agtctggcag ccgcacggaa atgtccgctt gccatcgctc ccgcgatgaa
      541 tgtggaaatg tggctcaacc ctgccaacca acggaatatc gcacaactgg tttcagacgg
      601 cattactgtc tatatgccgg gcttgggcga acaggcttgc ggagaaaatg gcatgggaag
      661 gatgccggaa cctgccgaat tgctggattt gcttccggac ttgtggacac cgaaaatttt
      721 aaggggcaag aaaatcttga ttaccgcagg tgcgacattt gaagccattg accctgtcag
      781 gggcatcaca aatacctcca gcggaaaaat gggggtagct ttggcgcggg cgtgccgtgc
      841 cgccggtgcg gaagtcagcc tgattcacgg acagcttcaa accgcgctgc ctttcggcat
      901 atccgatacg gttcaagccg tcagcgctga agatatgcat cgcgcagtgc atcgtttgat
      961 tgaaaaacaa gatgctttta tttctgttgc cgccgtctca gactataagg ttaaaaacaa
     1021 gagtacgcaa aaattcaaaa aagataaaaa tgccaaaccg ttatccatcg aattggatga
     1081 gaaccccgat attttggctt ctattgcctc attgccgaac ccgccgttct gcatcggttt
     1141 tgccgctgaa acggagaatg taatggcata tgcacgagaa aaacgtatta agaaaaaaat
     1201 accggtgata gttgccaatg atgtttcaat cgcgatgggc aaaccgacca accggattac
     1261 cattatcggg gacgacgggg aactatcttt tcccgaaaca agtaaagatg aagcggcgat
     1321 gcagattgtt gaaaggcttg ccggatattt gaacaaataa gcaattgaac ggataaacca
     1381 taaagcgggt tgcctgttaa tcaaaaggca acccgtttta cctgcttcaa cttctgatga
     1441 ctttgcggat atatggaata ctatgcagat tttgaataat ctgattcaat tgattcagat
     1501 tcttgacttt caataagaat ttgaattcga caaaaccttc cgttcccgac tgggatttag
     1561 acggtgtttc gaccgactca atgtccgcac cggaatcgga aatcgcttgc gccattaatg
     1621 ccaacaggcc gtggctgtct tccgattgga cttgaagccc gacacggtag ttctgcccgt
     1681 tcatattttc ccagtctgca tccagctgct gttcgggatc ggacttcaac aacgtcgggc
     1741 aggtatctct gtggataatc atgccttttc ccttaaccaa cagcaaacgg atggaatcgc
     1801 cgggaacagg gtggcagcac tctgcaaaat gaatatgccc gctttcctgc ccatcgactt
     1861 taatggaact gagcctgacc tcactaccga aatgctttcc tgccaattcg gcaatatgca
     1921 tagcgacata aacaggcagg gtatgcccca taccgacgtt gtacagcact tcctcaaatg
     1981 aagtctgttt atcgttgaga tcggcaaggt atttttcttt gatgccgtct gaaagcagga
     2041 catctttggg cagcaaactg gacagggctt tttgtaagag gttctctccc aaaacgaccg
     2101 catcgtgccg gttaaggttt ttaatatatt ggcgtatggc actgcgcgcc ctgcctgaca
     2161 cggcgaaatt caaccacgcg ggattgggtt tggcgtgttc ggatgtaata atttcgacgg
     2221 aatcaccggt tttgagcttc gtacgcaacg gcatcatgat attgttgata cgtgcggcaa
     2281 cggttttgtg cccgatatcg gtatgcaccg cataagcaaa atcgacaggc gttgcccctt
     2341 tgggcaaagt taggattttt ccttttggcg taaggatgta gatttcgttc ggaaacaaat
     2401 cgactttgac gtgttcgaga aactcaatgg cattggcact gcttgcctgc aaatctaaga
     2461 tatttttcag ccaccggttt gtgtgaagca ccgcctgatc gaccgtctta gaatatgatt
     2521 tatagctcca atgtccggcg attccacctt cggcaacagc atccatttcc ttggtacgta
     2581 tctgaacttc aatcggcaag ccgtaaggac cgaccaaagt cgtatgcaga ctttgatacc
     2641 cgttgctttt cggaatggcg atatagtctt tgaaccgccc gggcttgggc tgatagaggg
     2701 tgtgcaatgc gccgagtgcg gcataacagg ctggaatgct gttgacaatg acgcggaaac
     2761 cgtaaatatc cataacctcg gcaaagcgca gctttttcgc catcattttc tgatggatgc
     2821 cgtacaggtt tttttccctg cctttgattt tggcctctat attcgcgcct accagccgct
     2881 ggccgaatgc gcgcaagact ttgccgacaa cgtcctgccg gttcttccgg ctcttgtcca
     2941 tcgctttttt taaagtctcg tagcggttgg gatgcaggtt ttggaacgat aaatcctgaa
     3001 gctcttgata tgcgttattc aaacctatgc ggttggcaat ctgtgcatag atttcaaggg
     3061 tttcccttgc aatccggcgg cgtttgtccg ggcgcatcga accgagtgtc cgcatattat
     3121 gcagacggtc ggcaagtttg acgacgatga cgcgtacatc tttggtcatt gccaaaatca
     3181 gtttgcggaa actctccgcc tgatgctctg catgatcttc aaatttgagt ttttcgagtt
     3241 tggacagccc gtccaccatc tcggcaaccg tattgccgaa caccgccgcc atttcccctt
     3301 ttgtcacgcc cgtatcttcc aatacgtcgt gcatcacgcc tgcacaaaga ccctgtatgt
     3361 ccatatgcca aagggcgagc tgcgtcgcaa cggcaatcgg atgcgtgatg tagggctccc
     3421 cgcttttgcg ggtttgcccg tcgtgggcgc gaaacgcata ggcgacagct ttttcaagct
     3481 ccgcctgttc ctcgggcttg aggtaggagg cggtatggaa aagcagggca cgcgcttcgg
     3541 cggtcagggg gtcgtaaggt gcggtgggtt gagggacagg catttcagac ggctttcgga
     3601 acatatttta tttttcgttt caaaccgtcg gactgcacgg cggcagaatg tgccgacgtg
     3661 cgtttccggc agaatttatt tattgcgcgt caacagttcc gtaccgatat gcccggcggc
     3721 gatttccctt aaggcggtaa cggtcggttt gttattgcgg acatcgtcca caagcggcgt
     3781 attgccattt tcaagctggc gggcccgacg ggccgctacc aatgtcaggt caaaatggtt
     3841 ggaaattttt ccggtacagt cttcggtggt aatacgtgcc atattatttg ctttctttca
     3901 aaaatattta aaataggaaa ctgggtattt tcgcggtttt ttaggaattt tccaacaaat
     3961 ctgcaataaa tcccagttgc cgcgaccttt tcagacggca ggcattcaca atatggcgca
     4021 aatcctcctc cgcccgcgcc aagtcatcat tgaccacgac aaaatcgaac aatacggact
     4081 gttcgatttc atgccttgcc ttcgacagcc tcctttggat aacttcccga ctgtccgtcc
     4141 cgcgtccgtt cagacgtgca gcaagcacat cgaaagacgg cggcaggata aagatgccga
     4201 cggcttccgg cagcgcgttg cgaacctgcg ccgcgccctg aacgtcgatt tccaaaatta
     4261 cgtcatagcc tgccgccgcc aaagcattca cgccctccgc gcttgtgccg taatagttgc
     4321 cgaacacgtc tgcgtattcc aaaaaagctt cctgcgcgat aagcgactca aactcttctt
     4381 tagaaacaaa atgataatgt acgccgttta cttcgccttc acgcggcgga cgcgtcgtgt
     4441 gcgatacgga aacgcgcaaa ccgttatggt ttgccaacag ccgcgacacc agcgtggttt
     4501 tgcccgtgcc ggaagcggcc gaaatgataa agatgttgcc ttttcgataa gcggacatat
     4561 tttttacctg tatattttcc agccgattgt atcacaacgg acactcggtt tcctatttgc
     4621 cgatgcccat attttgccgc tattgttttg attgatttgg caagcggcag gctgacggct
     4681 acaatatggc gttaaaaaca tcaaacttgg aacacgcaat gctggttcat cccgaagcta
     4741 tgagtgtcgg cgcgcttgcc gacaaaatcc gcaaaatcga aaactggccg caaaaaggca
     4801 tcttattcca cgacatcacg cccgtcctgc aaagcgcgga atacttccga cttttggttg
     4861 atttattggt ttaccgctat atggatcaga aaatcgacat cgttgccggt ttggacgcgc
     4921 gcggcttcat tatcggcgcg gcactcgcct accagctcaa cgtcggtttc gtccccatcc
     4981 gcaaaaaagg caagctgcct tttgaaaccg tatcgcaaag ctacgcgctc gaatacgggg
     5041 aagctgcggt ggaaatccac accgatgccg tcaaacccgg ttcgcgcgtg ctgctggtcg
     5101 atgatttggt tgccacgggc ggcacgatgc ttgccggact ggagctgatc cgcaaactcg
     5161 gcggggaaat tgtcgaagcc gccgccattt tggaatttac cgaccttcaa ggcggcaaga
     5221 atatccgtgc aagcggcgcg cccttattta ccctgcttca aaacgaaggc tgtatgaagg
     5281 gctgaaaacc gaccctgccg tctgaaaccg gcagggttgt tattatgcgt tcaaatcacg
     5341 cccaaatctt gcaagcccct caacacaccg tcctcatcaa cgctggggca aacatatttc
     5401 gccgcctctt tcgccgcctg ttccccgttg cccattgcca cgccgaaccc gacttctgac
     5461 agcatttcca catcgttcaa accgtcgccg aacgccatca cgtctgccat ttccaaaccc
     5521 aatgccccaa ccacgcttct gatgccgtct gttttcgaca ttcccgcagg cagcagatcg
     5581 accgcttcct cgtgccagcg caccgttttc aagccttccc gttccacaat atccgaccag
     5641 ggcggcattt cgttttcctc cgcaaacacc agcatctgat acaccggttt gcttgaaaaa
     5701 taatccttat cggcaaaaaa atcgctggcg atatgctgca aggcgcggca cacgcattcc
     5761 gacagcgcgg acacggcgat cccctctccg ccgacaaacg cataatccat gcccaagcca
     5821 tccaaatgcg cgcaaaccct gcccatcaaa ccggcatcca tcggtacttc gcgcacagtt
     5881 ttaccgtgca gcagcgcaaa ctgtccgttt atcgttacca cggcatccat acccgtttcc
     5941 gccatcatgt ccctgacctt ttcgggaatc gtcgccaaag accgccccgt tgccaacgcc
     6001 gtcaatatgc ctttgccgcg caaagccgcc accgccgttt tcacggaagg acgcaaggta
     6061 tccgtatatt tgcggtacag cgtatcgtca atatcgaaaa acacgatttt aggattcatc
     6121 acattctctc tcgcattcaa actgccgcat tgtatcccaa tcaggcaaat acttgataaa
     6181 tccttataaa tttcccggca aaattgaccg cgaacacgaa aaggcggata atccgcccat
     6241 cctcaaaccc ttttcagacg gcatttgcag caatgccgtc tgaaacattt ttacaaagca
     6301 cacaaatcat gtttcaacac acaggacgac acataaagcg tcgccctata tgttgccctg
     6361 attcggaaag ggttacaccc ctcccaaata aagtctgatt ctaccgccct aaagggcggg
     6421 gtttcaaccg aaaaggaaac acgatgaaag cacccgaact cttattgccc gccggcggat
     6481 tggaaagaat gcgcgccgcc tacgattacg gcgcagatgc cgtttacgcc ggcagcccgc
     6541 gttactcact gcgcgcccgc aacaacgaat ttgccaaact cgacgttctc gaacaaggca
     6601 ttaaagaagc gcacgagcgc aacaaaaaat tcttccttac cgtcaatacg ctgccgcaca
     6661 actccaaact caaaaccttc gtctccgata tggagccctt gattgcgatg aagcccgacg
     6721 cgctgattat ggcggatccg ggtttgatta tgaccgtgcg cgaaaaatgg ccggaaatgc
     6781 cgatccacct gtccgtacaa gccaacacca ccaactactg gggcgtgaaa ttctggcaga
     6841 atatcggcgt cgaacgcatc atcctgtcgc gcgaattgag tatggaagaa atcgccgaaa
     6901 tccgccaaca atgccccgac atcgaactcg aagtcttcat tcacggcgca ttgtgcatcg
     6961 cctactccgg ccgttgccta ctctccggct acttcaacca ccgcgacccc aaccaaggca
     7021 cctgcaccaa ctcctgccgt tgggactaca aagtccacaa cgctacggaa agcgatgcgg
     7081 gcgatgccca gcttctgcaa ggtttcaact ttgaaaaagc ccaagaagaa gccaaccaaa
     7141 actttgaagg catcaacggt caaaaacgcc atccctacgc cgacaaagtt ttcctgattg
     7201 aagagtccaa ccgcccgggc gagatgatgc cgattatgga agacgaacac ggcacctaca
     7261 tcatgaactc caaagacctt cgcagtattg aagtggttga gaaactcgcc aaaatcggcg
     7321 tggacagcct caaagtcgaa ggccgtacca agtcgctcta ctacgttgca cgcgtggctc
     7381 aatcctaccg caaagcgatt gacgatgccg ttgcaggccg tccgtttgat tacagcctgt
     7441 tgagcgaact cgaaggcctc gccaaccgcg gctacaccag cggcttcctc gaacgccacc
     7501 aaacccaaga ctatcaaaac tacctgaccg gccactccat cgccaaacaa agccaatatg
     7561 tcggccacgt taccgaaatc gacgctgaag gttgggcaac ggtggaagtt aaaaaccgct
     7621 ttgccatcgg cgattcgctc gaaatcatcc acccgagcgg caaccaaacc atcaaattgg
     7681 aacaaatggc ccgaaaaggc cagcctgtcg atgttgcacc cggcaacggc atccaggtca
     7741 aaatccccaa tatgcggggc aaagaaaaag ccctcatcgc acgcgtattg aacccctgag
     7801 ccacaatgcc gtccgaaccc cgttttcaga cggcattttg ttgaatctta cggtacaatg
     7861 gcaatgattt cccaccgcaa gcaaggatat ccgacatgaa aaaaaatcct catactctcc
     7921 gccgccgccc tgatgctggc agcctgccaa tccggcagcg cacctaaaaa cgcgcacacg
     7981 gcatcccccg gcacaccggc cggagaatca tgcaaagtca tccggggcga caccgcagga
     8041 agcggcaaaa acatcattta ccgttgctgc aacggtcagg cggcaataaa tgcagccatt
     8101 gcagacggcg tattggaatc agggattccc gtcagcttcg gcggcggggg cgtaccggtt
     8161 gcaaacggca ggaatctagt tacccgtcag gcggcaaacg gcataggcaa atccgactca
     8221 gaagcctacg aacgcgccct aatcaatgcc gcccgcaaat tccaacagac cgcaggcaaa
     8281 ctcggcggac gcggcgttac cggcttccac agctactttg acaagcaacc gctgcaaggc
     8341 gggcaatacg actgtcaggc aggctcgttc cacgtccgcg tcgtcatgcg cgccaatgtc
     8401 gtccgttaac atatcggcaa tcaaaaaatg ccgtctgaaa cccgttttca gacggcattt
     8461 tttcaggctt taccggcaat tccttcaaca tcccactccg gctgtgactg taacactgta
     8521 gcagcaaggt atacggatat atagtggatt aacaaaaatc aggacaaggc ggcgagccgc
     8581 agacagtaca aatagtacag caagacgagc caacgctgta ctggtttaaa tttaattcac
     8641 tatattatca ctttccgtca tcctaaccat ataatatcaa acaaaagccc cggtctatcc
     8701 gcaccggggc tttcgtcaaa tcaaggattc aaacgggatt attgggtttt cttaccacca
     8761 aatacgccga agctgttatt tgtatatgtc ttggcctgtt ttggttcatc cttaacatag
     8821 ctgtacctgc ctgccatttc tgcagccttg gcaccataga atcctccaga gatactacct
     8881 ttctcaccgt ttttccaagt accggtaaag cggttgccat caagacttcc ctcgaattcc
     8941 taggtaccca tatacaatcc gtcaccactg ttaatcttac cgttcaggga tttaccggca
     9001 aaattgacag tagcagtaaa cgaaccggct gcacggaatg aattaccttc attaaaccaa
     9061 ttaccttcgg tcaagttgcg cgaacggaaa tgcaatacat cgccgctata agtaaccgta
     9121 tcgccgggca gactgtcgct caactttcct tgcacaaaga gtgcgtatga accatctgcc
     9181 aatttagccg caccataaat aagattttca aaaccttggg aagtaataat cttatcgaag
     9241 tctgtccaaa gcttcaccct ccttgacagc tgttcatcag ccactgtcaa attaatagtt
     9301 tcgccattga tgaccagctt cgtgatttca ccttccccgt aaaaatattt ttcacccaac
     9361 tcaccggttt tcaatggtga ggcagccggt gctgaaggtt cgggattggc aggcgccggg
     9421 tttgatgcac ccggaacagt cggggtaggc ttaacacttg gcgcgtctcc gcctcccgaa
     9481 ctacaagctg aaagagctag accgcaggcg atggcaagtg cactcaattt aaatgacgaa
     9541 ttcgacataa cattctccta atgacatgga taacaaaaat aagttcatcc tcttttacga
     9601 aaaggcatga tgcttattca taaatttatt gaaaaaaaat ttcaagtttt catagtcggt
     9661 tgcaaatatg ttccggcaaa cagctgcgta tcatataaag cgcaactcca taaaaacacc
     9721 gatacctgat ttttgcatta ctcaatgcct gtgcggcatc attccttccg gcgcttcggc
     9781 atcagcggcc aagccaaagg tttcgcgcaa cacgactttg taaaatgcaa aggcttcgcg
     9841 cgcgccttgg atggcttccg cttcggcttc gggagtcagg ttcaaagcgt tcagatgctc
     9901 gacgaaggcg cgccagtgtt tgccgcgacc gtcgggatgg ggagcgaggt ggcgcgcgcc
     9961 gtgttcgccg ttgtaatcga gtttttgggc gtgtttgaac aaaaacgccg cgcccaaatt
    10021 ggaaccttcg gcgcaataaa gccagccgat tgctttgttg ccggtttcgt gtggcaattt
    10081 tttgtcgaat ttgtaaggct cttcgcccaa gtctttcaag tcttgcgtta cggcatcgta
    10141 tcgcgccata tattccagct cgggaatggc tttgtttaat tcggcatctt tatagatgtg
    10201 gtcgacggcc ttgtggaaaa cggattggag tttcaaaaat ttaatgtagt tttctttgct
    10261 gacaaacggt tggacagaca taacgaggtt atccacgctg tcgtgaaccg ccgtggtatc
    10321 cgccttcaga cgtttggcaa acgtcagggc ttgattttcg gtttcactca taagttttcc
    10381 tttgtcggta agggataagg atgcggggcg ggcaaaatcc gcacttgccc g
//
LOCUS       DAXHTR010000072        10423 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.072, whole genome shotgun sequence.
ACCESSION   DAXHTR010000072 DAXHTR010000000
VERSION     DAXHTR010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10423
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.072"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            123..305
                     /locus_tag="ACFNML_001249"
     CDS             123..305
                     /locus_tag="ACFNML_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392632.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNVGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(367..1368)
                     /locus_tag="ACFNML_001250"
     CDS             complement(367..1368)
                     /locus_tag="ACFNML_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase"
                     /protein_id="HFC6392633.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1445..5278)
                     /locus_tag="ACFNML_001251"
     CDS             complement(1445..5278)
                     /locus_tag="ACFNML_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3683 domain-containing protein"
                     /protein_id="HFC6392634.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALIREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWQMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGLNIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITGVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKVRYGSA
                     VHAAPEAKDNESCFTAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLGGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSIKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     AMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGKA
                     PIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5539..5635
                     /gene="ffs"
                     /locus_tag="ACFNML_001252"
     ncRNA           5539..5635
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACFNML_001252"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            5803..7494
                     /gene="dld"
                     /locus_tag="ACFNML_001253"
     CDS             5803..7494
                     /gene="dld"
                     /locus_tag="ACFNML_001253"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFC6392635.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDIKQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPPEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            7626..7733
                     /locus_tag="ACFNML_001254"
                     /pseudo
     CDS             7626..7733
                     /locus_tag="ACFNML_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            7789..8160
                     /locus_tag="ACFNML_001255"
     CDS             7789..8160
                     /locus_tag="ACFNML_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HFC6392636.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVEAANPQAALAQYFGRGGR"
     gene            8315..8863
                     /locus_tag="ACFNML_001256"
     CDS             8315..8863
                     /locus_tag="ACFNML_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6392637.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            8997..10088
                     /locus_tag="ACFNML_001257"
     CDS             8997..10088
                     /locus_tag="ACFNML_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HFC6392638.1"
                     /translation="MNAQTLIANVAEFVKTKLKPIVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNRYLDDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLTTLKLREAAAVLTLAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
BASE COUNT         2420 a         2876 c         2813 g         2314 t
ORIGIN      
        1 atttgtactg tcttcggctc gccgccttgt ccttatttaa atttaattca ctatatttgc
       61 ccgtgcattt gtgataaatt cggcactgta tttgatatga ttttaacaaa tcgggagggg
      121 caatgttttg gtatgtaatc ggcttttgct cctttgtcgt cgcgctgctg tcgctgtggg
      181 tcaatgtcgg cgcattcggt atgcaggaag acgatacccc gcaatcggat tatgaaaaac
      241 gtttgggctt gggtgcgaag ctgaaaaaca aaaatacgcc ggatacggaa gaaaagcagc
      301 aataaagata tgaaatgccg tctgaaagat tttcagacgg catttgcttt gaagcatggg
      361 agacagtcaa acatttatct catgctcaaa tgcgttcagg attcggcgga cttcatccgc
      421 atccttaacc gttcggacgc ggtcaaacag cgtttgcgcc gggtcgaacg tttttttcat
      481 catgcccaac cattgcttaa gacgggcgat cgggtattta ttgtttgcct cttttgtcag
      541 acacagctcg aaaaactgcc gtatccattt ggaaacttcg gcaaaatccg tgtctttgac
      601 cggcccgccg ttctcgtatt gcttgatttg ccgcgccaaa tcggggcgga tgaccgcgcc
      661 gcgaccgagc atcacgctgt tgcagccgct gattgttttg atgccgatat agtcttgcag
      721 gctgaaaacg tcgccgttgg cggtaacggg aatattgacg ctgtctcgga ttttccttat
      781 ccattcccaa tgcgccggcg gttcgtaacc ctcggctttg gtacgcgcgt gtacggtcag
      841 tccgcatgcg cccccttcgg caatcgcaca ggcgcattcc aaagcccggc ttttgtcttc
      901 gtaaccgagc cgcatttttg cggtgagcgg aatatgtgcg ggcaaacgtc cgcgcagcgt
      961 tttgacgatg tggaatatca gttccggctc ttttaaaagg attgcgccgc ctttgtgttt
     1021 gttgacggtg ggcgcggggc agccgaagtt caaatcgatt ttgttcgcac cgaagcggac
     1081 ggcttccagc gcattcgccg ccatattgtc cgcatcgctg cccaaaagtt ggacggtgca
     1141 gggcgtgccg gaaaacgttt tgtttccgtt ggcgatttcg gggacatatt ttaaccatat
     1201 ggatcgggaa tgcacggtat gggtaatgcg tacaaattcg ctgacgcatt cgtcgtagcc
     1261 gccaatacgc gtcagcaggt cgcgcatcac gtcatcgacc agaccctgca taggggcaag
     1321 gatgattcgg gttttttgtt tgggttcggt tgtttgtccg tcaatcatga tttgggtagg
     1381 tattgggttt aaacaatgcc gtctgaaggc tttcggcgtt cagacgacat ttccgtgtcc
     1441 gttgttacag caatactttt tccacgccgc cgttgttggc ttttttcaca aactcgtcta
     1501 gccagttttc gccgagaatg tgtttcgcca tttcgatgac gatgtagtcg gcaggcatat
     1561 tgttgtcgtc ggcgtagcgg ctcaaacctt gcaggcaggc ggggcaggaa gtcagcattt
     1621 tgacgggttc gccctgcggc agctctttga ggtttttctc gatttcctct tgtttgcgga
     1681 acttgacctg agtggcgatg tctggccgtt tgacggcaaa cataccggat tcgccgcagc
     1741 agcggtcgct taaaacgact ttctgtccca tcaggctgct ggccatttgg gtggcgttca
     1801 tggttttaat cggggtgtgg caggggtcgt ggtagaggta ttgctgacct ttcacgccgt
     1861 cgagtttcac gcctttttcg agcaggtatt cgtggatgtc gatgatgcgg cagccaggga
     1921 agatttcctc gaagcggtat ttttcgagct ggtcgtaaca agtgccgcaa ctgacaacga
     1981 cggttttgat gtcgaggtag ttgagggtgt tcgccatacg gtggaaagcc acgcggttgt
     2041 tggtgctcat ttcttcggct ttagccttgt tgccgcccgc gtcttgcgga tagccgcaac
     2101 acatatagcc gggcggcagg acggtttgta cgccgacgtg ccagagcatg gcttgaacgg
     2161 caagtccgat ttggctgaac aggcgttccg aaccgcaacc cgggaagtag aacacggctt
     2221 cggcatcttc gggcgcggcg gggttgcgga tgatggggat gcttttgccg tcttcgatgc
     2281 ccaataagga gcgcggtgtt ttagcgggta cgcttttggg cagtgggcgg ttgatgaaat
     2341 ggataacctg ttctttaatc ggggctttgc cgacggttgc cttgggttcg gctttttgct
     2401 ttttcgtgcc gatcggaaga agcttgccga ttttgtaggc aaagttttgc gcggggaagc
     2461 cggtttgtat catggcggcg cgaagggctt tgatggtttt cgggccggtg gcgttcaaaa
     2521 acgccatacc catagctgcg gcaggcgcaa atcgtttgtg gccggaatcg gcaaggtagt
     2581 tgcggacggc tacggttacg tcgccgaagt cgatgttgac ggggcagggt ttgacgcagc
     2641 ggtggcacac ggtgcagtgg tcgccgatgt ccatgagttc ttcaaagtgt ttgatggaaa
     2701 cgccgcgacg ggtttgttct tcgtataaga aggcctcggt cagcaaaccc acgccgagga
     2761 ttttgttgcg ggggctgtac agcaggttgg cacgcggaac gtgggtggag cagacgggtt
     2821 tgcatttgcc gcagcgcagg cagtctttga cggaatcggc aatcgtgccg aggtctgatt
     2881 tttccataat cagcgattcc gcgcccaaaa gctcgaaaga cggcgtgtag gcgttgcgta
     2941 agtccgagcc tttcatcagt ttgtgacggt tgaaggtgtg tttgggatcg acttggtttt
     3001 tgtagtccca aaacggctgc atttcttcgt cgcttaaaaa ttcgagcttg gtgatgccga
     3061 tgccgtgttc gcccgaaatc acgcctccga gcgaacgggc gattttcata atgcgttcga
     3121 ctgagcggta tgccgtctga agcatttcgg catcgtctga gttaaccgga atattggtgt
     3181 gaacgttacc atcgccggcg tgcatatgca gggcgacaaa gacgcggctg cgtacggttt
     3241 ttgcgtggat ttttcccaag ccttggataa ttttggtgtc agttttgccg ctgaagattt
     3301 cagaaagcgg cttcattacg tccgctttga cagacacgcg caggcggaaa tcgcggaatg
     3361 cggtaaagca gctttcattg tctttggctt cgggcgcggc gtggacggct gagccatagc
     3421 gtactttata gtcggcaagc ggcgcgtcta agttagcaag cagccattcc caacgcgctt
     3481 tgacggcgga aacgtgggca agggcgtgtt tgccgcgttc gcccaaaagt tcggcggtcg
     3541 gcaggtcggt tcccattttg tcgatgggga gtttgccgga cagatattgt tccaaggcgg
     3601 cacagagttt gagtttgttt tggatggaaa gctcaatgtt aatgcgttcg atgccgtctg
     3661 aatactcacc gagcctttcg agtgggatga ccacgtcttc gttaatttta aaggcgttgg
     3721 tgtgtttggc gatggcggcg gtgcggctgc ggtcgagcca gaaggttttg cgcgcttcgg
     3781 gcgatacggc gataaagcct tcgccgtcgc gggcgcgggc aagttcacag atgtgctcgg
     3841 cggctgcctc tacggcggct tcgtcgtctg aaaccacgtc cgccagtaag accattttcg
     3901 gccggccttt gcctgccgct ttggtggcgt agccgacggc gcggacataa cgccagtcca
     3961 aatgttccaa acctgccagc cgaacgctgt cgtgggcgag caggaaatcg cggatttcga
     4021 cgatagaagg cgtagcggtg gcgaccgtgc cgaaaaactc catacacaca gtgcgcgtgt
     4081 atttcggcat tttgtgcaac acgaaagcaa cgccggtgat gatgccgtcc gtgccttctt
     4141 tctgtacgcc gggcaggccg ctcaagaatt tgtcggtaac gtctttgccc aaaccgactt
     4201 tgcgaaattt gtggccggga atttccaaac gttcggtttt aacgatattg aggccgtctg
     4261 aatccagcgt gtgaacgtcg aacacggcgg tttcttcgtc gtggattttg ccgaaattgt
     4321 ggcgcacgcg ctcgatgcgc agccattcgc cctgcgggtt gaccatctgc cagtaggcga
     4381 ggttgtccaa cgccgtcccc cacagcacgg cttttttacc gcccgcgttc atcgccacat
     4441 taccgcccac acaggacgca tcggcggaag tcggatcgac ggcgaacacc aaacctgcct
     4501 gatgcgcggt ttcttccacc cgccgcgtaa ccacgcccgc gccgcaccgg ataatcggat
     4561 gcttgccgtc caagcctgcc agctcaacgt attcaacacc acgatgcttg tcgagttttt
     4621 cggtattgat gactgcgctg tttgcgtcca aaggaatcgc gccgccggta taacccgtgc
     4681 cgccgccgcg cggaataatg accaaatcca gctcgattaa ggcgcgcacc aaaggcgcga
     4741 tttccgcctc cgtgtcggga ttgacgacga caaacggata ctccacgcgc cagtcggttg
     4801 catcggtaac gtgcgttacc cgcgccaatc cgtcaaacat aatattgtgc ggcttggtga
     4861 ttttgctcaa acgctccaaa atctgccgcc gcttttggct ggtttcatca aaactgctat
     4921 caaaacgctc gactgctttt tctgctgcgg caaccaagac atccacttgc cgattatcgt
     4981 cgcggcgttt gcggatttcg ttcaggcggt ggcgcatttc gcgtatcaac gcggcgcggc
     5041 gtttcggatg ctccagcaaa tcatcgacca gatacggatt gcgcacgacc acccaaatat
     5101 cgcccaacac ttcaaacagc atccgcgccg aacgtccagt tttacgctgt ccgcgcaagt
     5161 cttgcagaat ctgccacgcc tcgtcgccca ataatcggat gacgatttcg cggtcggtgt
     5221 aggaagtata gttgtagggg atttcccgaa tacgctgagg ggcggtagtc gtggtcatgg
     5281 tgtgtccgtg tttggcagtt tttatttgaa tgccgtctga aaagtcggtt tcaggcgagt
     5341 ttgaaaaggc gataatgtag ttcaattttt gatacttttc aatgccgaaa caggaaatac
     5401 ccttatagaa acaaaataac gctttgagaa attgttgaca atattatcaa atataaattt
     5461 taggtggtgt ttccggcgta tgaccgtatc aaaacggttg agtttttcag aaaacccgtt
     5521 ataatgccgt ttctctgagg cgggtctccc cgcatggcaa atcggaacac cgggtcaggg
     5581 gcggaagcca gcagcccact ccgatgcgcc agtgccgggg gtttggcccg ccgccctatt
     5641 tgaaacgccg gagcttcgat gttccggcgt tcccgtatcg caccgatgcc gtctgaaagc
     5701 cgttcagacg gcatttcctt tactttgcca taacgcataa ctacaatggg aaaacaattt
     5761 caatgtgcat atgcacctgc cgacaatttt aaggaaactg ccatgagcgc atcacaatta
     5821 ctgtcaagac tgacccaaac cgtaggcgaa aaatacatca tcaccgaccc cgcgaaaacc
     5881 gaacaatacc gccaaggcta ccgcttcggc gagggtaagg cgttggcggt agttcgcccc
     5941 ggaagcattc tggaaatgtg gaaaattctg caggcctgcg tcgaagcgga cgtgattgtg
     6001 attacgcagg cggcgaatac cggtttgacg ggcggctcga cccccgacgg caacgattac
     6061 gaccgcgaca tcgtgattgt gaacaccatg cggatgaaca tcatccaaac catcaacaac
     6121 aacgaacaag tcgtctgcct gcccggttcg accctgaacc agcttgaatt gctgctgaaa
     6181 cctttggggc gcgaaccgca ttcggtcatc ggctcatcct gtatcggtgc gtccgtcttg
     6241 ggcggcgtgt gcaacaactc cggcggcgcg ttggtacagc gcggcccggc ctacaccgaa
     6301 atggcgttgt tcgcccaaat caacgaagaa ggcaggttgg aactggtcaa ccacttgggc
     6361 atagacttgg gcgacacgcc cgaagaaatc ctgaccaacc tgcaaggtca tcattatcaa
     6421 aacaaagaca tcaaacaaga cgcgggcaaa gggcacgacc acgcctattg cgaacacgtc
     6481 cgccaagtgg acgaaccgac cgccgcgcgt ttcaatgccg accccgcccg ccattacgaa
     6541 gcctcgggct gcgcgggcaa actgatggtt tttgccgtcc gtttggacac cttcccgcaa
     6601 gaaaaacaaa ccgccgtgtt ctacatcggc acgaacgaca tcaacgagct gaccgacatc
     6661 cgccgcgccg ccttgggcga atttgaaagc ctgcctgttt ccggcgaata catccaccgc
     6721 cacgctttcg acattgccga cgtgtacggc aaagacacgt tttacgtcat caaaaaattc
     6781 ggtacgcacc aactgccgaa attatttgac ttgaaggcgc gcgtggacag gttcggcaaa
     6841 aaagccagct tcctgcccaa acatttttcc gacaaggcca tgcagttcgt cagcaaatac
     6901 ctgcccgacc acctgcccaa atccatgcgc gattaccgcg acaaatacga acaccacctg
     6961 attctgaaaa tgggcgggaa aggcgtggat gaggcgcgcg cgttcttaaa agaatatttt
     7021 gcacaccaca gcggcgcgtt tttcgagtgc aacgccgaag aaacccaagc cgcgatgctg
     7081 caccgtttcg ccgtcgcctc cgccgccatc cgctaccgcg ccgtgcatga tgacgaagtg
     7141 gaagacttgg tcgcgcttga catcgccctg cgccgcgacg accgcgactg gtttgaaaaa
     7201 ctgccgcccg aaatcgacaa taaaatcatc cacaaactct actacggaca tttcatgtgc
     7261 cacgttttcc atcaggatta catcatcaaa aaaggcaacg actgcatggc gttggaacac
     7321 gaaatgctgc atctcttaga ccaacgcggc gcgcaatatc ccgccgaaca caacgtcggt
     7381 catttgtatg aagccaagcc cgcgctcaaa cagttttacc gcaaactcga cccgaccaac
     7441 agcttcaacc cgggtatcgg caaaaccagc aaaaagaaaa actgggcgga ataagcgcgt
     7501 ccgctttgaa ggcaggcaat gccgtctgaa ggcaaaacgc cgttcagacg gcattgtcgg
     7561 gtttgaagtt ccccgtcggg taatcgcttt caaaccgtat gccgacacac gggcgcgccc
     7621 ctcccaaata tagtggatta aatttaaatc aggacaaggc aacgaagccg cagacagtac
     7681 agatagtacg gcaaggcgag gtaacgccgt actggtttaa atttaatcca ctataaagtc
     7741 tgatcctacc ttcccaaagg gcagggtttc aaccgaaaag gaaacacgat gaaaccatac
     7801 aaaatctaca cccatcccgc cctgccgccc caagccgtca aacaaggctg gtcatggccg
     7861 gggctcttgt tcggcacgct gtgggcgtgt ttcaaaagaa tgtggggttt ggggctgggg
     7921 ctgacgggcg cgatattcgt gctggctgtg tttgcccaac tggtttacgg cgacacgccc
     7981 gccacagact cggcgtttaa cgtattgggc ttggcggttt ccgtctggtt cggcgcaaag
     8041 ggcaacagcc tttatgcacg ccacctttta tcgcgcggct ataccgaatt gcccgaaacg
     8101 gttgaggcag ccaatcccca agccgcattg gcgcaatatt tcgggcgcgg gggccgataa
     8161 gcgcgttttc ttttccatca tgccgtctga aaaatgttca gacggcattt cttataacct
     8221 tttgtttgca gcacataacc aatcagtctt tcctattccg ttccgaaatg cataccatgc
     8281 gcccgtaccc aatatttgcg aacaaggaaa gaaaatggca cgtttaaccg tacacaccct
     8341 cgaaaccgcc cccgaagccg ccaaagcgcg cgtcgaggcg gtacttcaaa acaacggctt
     8401 tatccccaac cttatcggcg tattatcaaa cgcccccgaa gccttggcgt tttaccaaga
     8461 agtcggcaag ctcaacgccg ccaacagcct gaccgccggc gaagtcgaag tcatccagat
     8521 catcgccgcc cgtaccaacc aatgcggttt ttgcgtggca gggcacacca aactcgcaac
     8581 cctgaaaaaa ctcctgtccg aacaatccgt caaagccgcg cgcgctttgg cagcaggcga
     8641 atttgacgat gccaaactcg gcgcgctcgc cgcctttacc caagccgtaa tggcgaaaaa
     8701 aggcgcggta tccgacgagg aactcaaagc atttttcgat gcgggctaca accagcagca
     8761 ggcagtcgaa gtcgtgatgg gcgtagcctt ggcaaccctg tgcaactacg tcaacaacct
     8821 cggacaaacc gaaatcaacc ccgaattgca ggcttacgcc tgatacggca aaccgcccga
     8881 atatcgggcg gtttttcaaa aacaccccct caaacaaaaa caagccgcca agcggcaggg
     8941 aagcagccgc gcatcaggcg cgcccccgat tcccccgcaa accttaagga acaaagatga
     9001 acgcccaaac cctgattgcc aacgttgccg aattcgtcaa aaccaagctc aaacccatag
     9061 tggacgacat cgaccgcaaa ggatactacc ccgaagcatt tatgcgcgaa ctcggcgcaa
     9121 tcggtggatt cggcgcagtc ggtacagaag ccgaaggcgg caacggcttg ggtttggcaa
     9181 cgcaaatcgc cgtattgcgc gaaatcggca aagaatgcgg cgcaacctct ttcagtgcgt
     9241 ggtgtcaggc ggcttgcgcg tggtatctgc accaaacgcc caaccgggcc gtcaaagaca
     9301 aataccttgc cgacatcctg caaggcaaag tattggcggg tacgggcatg tccaataccg
     9361 tcaaacacct tgccgacatc gaaaaacaca acctccaagc cgagcgcgtg gagggcggct
     9421 acacagtcaa cggcgcgctg ccgtgggtgt ccaacatcgg cgaagaccac atttgggcga
     9481 ataccgccca aatcggcggc ggctacgtca tgttcatcac aggcggacaa tgggaaggcg
     9541 taagcctgca aaactgcccc gaattttgcg ccctcgaagg cacgcgcacc ttcagcctga
     9601 acttcaaaga cgtatttatc cccgacgaag acatcatcgc cgcgcccgaa cagtttgccg
     9661 actacatcca aagcatcaaa gcaggcttta tcctcctgca aatcggcatc ggcgcgggcg
     9721 tgatagacgg cagcctcggc atcatccgcc tcgccaacgt cgtcaacgcc gaagtcaacc
     9781 gctatctcga cgacggctac gacagcctca aaacaaggct ggacggcgcg tgggcagaaa
     9841 ccgaacggct cgccggattg gcttggagcg gcacgcccga caaccttacc accctcaagc
     9901 tgcgcgaagc cgccgccgta ctgacacttg ccgccgccca atccgccgcc ctgcattccg
     9961 gcgcaaaagg ctacctgatg cggagtcccg cccaaaggcg cgtccgcgaa gcaatgtttg
    10021 tcgccatcgt aactcccgcg atcaaacacc tccgcaaaga aatagcggca atcgaagccg
    10081 caaaataaac aggcggaaaa aaccgaaacc cccatcgcgt cattcccgcg aaagcgggaa
    10141 tccagccccc aacgtaacag gaatccatcg gaaaaaacag aaaccctcaa accgtcattc
    10201 ccgcaaaagc gggaatccag ccccccctaa cgcaacagga attcatcgaa aaaaccgaaa
    10261 cctccaaacc gtcattccca cggaagtggg aatccagtaa ccgaaaaacc acaggaatct
    10321 atcgaaaaaa aacccaaccc ccaaaaaaac cgggcggata cctcaccatc ctcccaaacc
    10381 ctgatttata gcggatgaac aaaaatcagg gcaagacggc gaa
//
LOCUS       DAXHTR010000073        10142 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.073, whole genome shotgun sequence.
ACCESSION   DAXHTR010000073 DAXHTR010000000
VERSION     DAXHTR010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.073"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFNML_001258"
     CDS             complement(108..2711)
                     /gene="pepN"
                     /locus_tag="ACFNML_001258"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFC6392639.1"
                     /translation="MSKIVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSAKLLSVKINGAAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASAGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GEYSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPASYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGRL
                     KNNSFELTIKQTVPPTPDMADKQPMMIPVKVGLLNRNGEAVAFDYQGKRATEAVLLLT
                     EAEQIFLLEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIELPKHEKLLAAVEKVISDDLLDNAFKALLLGVPSEAELWDGA
                     ENIDPLRYHQAREALLDTLAVRFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALIGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHAED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(2847..3641)
                     /locus_tag="ACFNML_001259"
     CDS             complement(2847..3641)
                     /locus_tag="ACFNML_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HFC6392640.1"
                     /translation="MTTILAFDIETVPDVQGIRTLYELPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWAEFVQAWK"
     gene            complement(3757..3891)
                     /locus_tag="ACFNML_001260"
     CDS             complement(3757..3891)
                     /locus_tag="ACFNML_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392641.1"
                     /translation="MPSERIFRSVLRLVGVSSETVKQKFAGVELSYAGKAAGESLYCL
                     "
     gene            complement(4391..5259)
                     /locus_tag="ACFNML_001261"
                     /pseudo
     CDS             complement(4391..5259)
                     /locus_tag="ACFNML_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
     gene            complement(5310..5846)
                     /gene="ruvC"
                     /locus_tag="ACFNML_001262"
     CDS             complement(5310..5846)
                     /gene="ruvC"
                     /locus_tag="ACFNML_001262"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFC6392642.1"
                     /translation="MSATVRILGIDPGSRVTGFGIIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVTVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQADAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(5849..6088)
                     /locus_tag="ACFNML_001263"
     CDS             complement(5849..6088)
                     /locus_tag="ACFNML_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HFC6392643.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6118..7128)
                     /gene="dusB"
                     /locus_tag="ACFNML_001264"
     CDS             complement(6118..7128)
                     /gene="dusB"
                     /locus_tag="ACFNML_001264"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFC6392644.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVRAAGVPVTLKTRLGWHDDHQNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEARYELIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECAAAILNHIRAIHAFYGDTAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
     gene            7494..8867
                     /locus_tag="ACFNML_001265"
     CDS             7494..8867
                     /locus_tag="ACFNML_001265"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6392645.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQGY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            9104..>10142
                     /locus_tag="ACFNML_001266"
     CDS             9104..>10142
                     /locus_tag="ACFNML_001266"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6392646.1"
                     /translation="MVLFELRRGNAVLVFVNPLYQVCVLEKQHFPRFVIGESLLPHCM
                     EMLEEAGFADAVRAEPGFQLKNGAAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDK
                     ILIEEAAKQGVEVRFGHGVTAFDNSGDFVRLNIETDTGKRYELTAKFVLDASGYGRVL
                     PRLLNLETPSHLPPRQAHFTHIDDNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNR
                     CSVGVVGTPDKLAGESETVLKKFVYECPMLNEILDKAVWENDFPFRSIQGYSANVKSL
                     HGRHFALLGNAAEFLDPVFSSGVTIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMI
                     GVDAFRTYSGLN"
BASE COUNT         2344 a         2802 c         2619 g         2377 t
ORIGIN      
        1 atatgacttt gcggccgttt tgccattgcg taataaaacg atggggaagt gatgataaaa
       61 cgtgtgtgta actatatcag acggcattgt ttttctgttt gacggcctca atccaaaatt
      121 ttgccgacga tttcgcccac gtctttcgac aatccttcct gcgcccgaat gcgctgcaat
      181 gcttgtttca ccaagttttt gcggtgcggc tcgagcttgt tgcagaggtt gaacgcctgc
      241 accaaacggg cggcgacctg cgggttgaag cggtcgattt cgatgacttt gtcggcgatg
      301 aagcggtagc cgctgccgtc ttctgcgtgg aaatgcggga cgttgcggct gaagctgccg
      361 atgagcgaac gggctttgtt ggggttttcg aggctgaatt tcggatgctg caaggcggtt
      421 tgaacctgct gcagggtgtc gctgcggcgg cttgagccga taagggcgaa atatttgtcc
      481 atcaccagcg cgtcgtctga aaacttgtcg gcaaactgcg ccagcaggcg gttgcgcgta
      541 tcgctttcgt tgccgttgac ggcggacagg atgccccatt cgtgggtcat gttttgcgcc
      601 atttcgccgt atttttcggc aacggtttcg atgtgcgcgg ggtcggcgcg caggacgaag
      661 gcgcggcaga cgttgcgcag cgtgcgccag ccggcagctt cggggctgta ctcgtagctt
      721 tggttttcct gcttcgccgc ctgacggttc aattcgtgcc atttcggcag gaagcggacg
      781 gcaagcgtgt ccaacaaggc ttcgcgcgcc tgatggtagc gcagcgggtc gatgttttcc
      841 gcgccgtccc acagctcggc ttcagacggc acgcccaaaa gcagggcttt gaaggcgttg
      901 tctaagaggt cgtctgaaat gactttttcg acggcggcaa gcagtttttc gtgtttcggc
      961 agttcaatac cgtctgaaag tgcggcaagg ttggcggcga cggcacggcg gtagagcgtt
     1021 tgtgcggctt cccagcgcgt gaaggcgtcg ctgtcatggg cgagcagaag cagcaggtcg
     1081 tcgtcgctgt acggatagtt cagatgcacc ggcgcgctga acccgcgcag cagcgaggga
     1141 acgacggctt cggttacgcc ttcgagcagg aagatctgtt cggcttcggt cagcagcaat
     1201 acggcttcgg tcgcgcgttt gccctgataa tcgaatgcca ccgcttcgcc gttgcggttc
     1261 agcagcccga ctttgacggg aatcatcatc ggctgcttgt ccgccatatc gggcgtgggc
     1321 ggcacggttt gtttgatggt taactcgaaa ctattgtttt tcagacgacc ttccgcttcc
     1381 aagacgggcg tacccgcctg gctgtaccac aaggcgaact ggtcgagatt gatgccgttc
     1441 gcgtccgcca tcgccgcgcg gaaatcgtcg caggtcacgg cctgcccgtc gtgtcgttgg
     1501 aaatagagct tcatgccttt ttggaagccc tcttcgccga gcaaggtgtg atacatccgc
     1561 accacttccg cgcctttttc ataaacggtc atggtgtaga aattgttcat ctcctcatag
     1621 ctggcggggc gcaccggatg ggcggtcggg cctgcgtctt cggggaactg gtgctggcgc
     1681 agcaggcgga tgttctcgat gcggcgcacg gcgcggctgg cgcggtcgcc ggaaaattct
     1741 tggtcgcgga acacggtcaa cccttctttc agcgaaagct ggaaccaatc gcggcaggta
     1801 acgcggttgc ctgtccagtt gtggaaatac tcgtgtccga ccacggattc gatgccttca
     1861 aaatcggtat cggtggcggt acggctgtcg gcaaggacga acttggtatt aaaaatgttc
     1921 agccccttgt tttccatcgc gcccatattg aaatcgccca cggcgacgac catgaaaata
     1981 tccaagtcgt attccaaacc gaagcgcgtt tcgtcccact tcattgcgtt tttcagcgat
     2041 tccacggcaa agccgacctt gggcttgtcc gcttcggtgg tgtaaaactc gattttgacg
     2101 tttctgccgc tcatggtggt gaaatagtct tccgtgaccg ccaaatcgcc cgcgaccaaa
     2161 gcaaacagat aactcggttt ggcaaacggg tcttcccatt tcacccaatg gcggccgtct
     2221 gaatactcgc cgccgtcgat tttgttgccg ttggagagca aaacgggata gcgttttttg
     2281 tccgcgacga tggtggtcgt gaacttggac atgacatccg gacggtcgat atagaacgtg
     2341 attttgcgga agccctccgg ctcgcactgg gtaaacaggt taccggcgga cgcatacagc
     2401 cccatcagcg atttgttttc cgccggcagg atttcggttt ccacttcgac ggtgaagcgt
     2461 tcggacggca cgtctgcaat cgtcagcgtc tcaccttcca acacataatc cgccgccgcc
     2521 ccgttgattt tgacggacaa gagtttcgcc gaaccgtcca acaccagcgg ctcccctacc
     2581 ctctgcggct caaccgtcaa acgcgacttc acgacggttt gcggttcggc aatatcaaaa
     2641 tgcaggtcgg tttcaaggat gcggtaggca ggcgtttggt aatctttcag ataacggacg
     2701 attttgctca tttttttctt tcaatattgt ttggtttgac tggaaaaggc ttcagacggc
     2761 ataggcaaac cctgcaccga tatgccgtat gaaaccgaaa catcagtgcg gacgcaccgt
     2821 caaacaaccg gttttaattt caatctttat ttccacgctt ggacaaattc tgcccaatgc
     2881 ggtttgtctt ctgtttgggc ggaaagatag tttctgatcc gcttgatttc catttcgtat
     2941 tcgtccgcgt cgaatctgcc gctgaccaga cagaaacgca gatacatcaa atacgtattc
     3001 acggcatcgg tttcacaata attgcggatt tccttcagcc tgcccgcgtg gaacgcatcc
     3061 cacaccttgc tgccgtccat acccagcttg cctggaaaac cgcacagttt cgccatatcg
     3121 tccagcggca cgtttgccct cggctggtaa agcgcgagca aatccatcaa atcgcaatga
     3181 cgttggtggt agcggctgat gtagttgttc cacttgaaat cgcggctgtc gccgaaatcg
     3241 ccgtcaccca tatcccaata gcgcgcggcg ttgatgccgt gtagcagcga acggtaatgc
     3301 agaaccggca ggtcgaaacc gccgccgttc caactgacca gttgcggcgt atgtttttca
     3361 atcaactcga aaaatttggc aatcacgact tcttcgccat cgtccatctc gccgattgta
     3421 ccgacatgga ctttatcctg cccccaacgc atgcagcacg aaatcgccac aatctgatga
     3481 agatgatgct gcataaaatc cccacccgtc tgagcacggc gtttttgctg ggcaaacagc
     3541 accacttcat cgtcgggcag cgaggacggc agctcgtaca atgttcggat accctgcaca
     3601 tcgggtacgg tttcaatatc gaaagccaaa atcgtggtca tgacagcacc ttgtatttaa
     3661 aacagacttg cgcctattgt gtcattaaaa ggcggataaa aaaagaggca accccccaca
     3721 ggattgcccc aatacctcaa atcagagatt tacgcttcac aaacaataca ggctttcgcc
     3781 tgcggctttg cccgcgtagc tcaactctac gccggcaaac ttttgtttca ccgtttccga
     3841 tgaaaccccg accaatcgca agactgaccg gaaaatcctt tcagacggca tttcctgcct
     3901 gtcgtgtaat tccatgtagc gaaatgtacg ccattttcta cgctttgcca agcatttttt
     3961 acaatataaa tgtcaaaaca ttaattttat aaaattgctg aaaatattaa atatatggat
     4021 ttttattttt tatatttcaa taaatataaa tttaattttg atttatattt aaatttaagc
     4081 ataaaatgtc aaatattaaa ggaaacatga aaggcatata ttaaatattt atttataacg
     4141 ctatgttttt aaagaaaatt aattttaata tattaactag attgtctgca tatattcata
     4201 ggtttgcggt atttcttcca aaacctgctt cgaatttccc gaccaagtct taaaaatatt
     4261 gttttttgag atacttaaat agcagcgatt atcaaatgaa atctgttcat ataatctgcc
     4321 attttgcatt taaaaaaaca atcaggagtt tcgactcgaa acgcctgata tgttttgtaa
     4381 ttttacgtag tcagtaaaaa tcggggctgc cttccggacg ggttttaaaa cgcttgtgca
     4441 gccaaaaata ttgttccgga tgttcgcgca ccctgtcttc gataaaacgg ttcatgcgct
     4501 gcgcgtcggc tttcgcgtct tcacccggaa aggatttcca agcagggtag aaatgcaatg
     4561 taaccgtatt gtctgcctcg cggacgggaa tggcgggtat cacttttgca tttgcaagcg
     4621 cggcaatgcg gctcaatccg gtaatcgttg ccgtctgaat accgaaaaaa tccacaaaaa
     4681 ccgaatcgtt gcgtccgaaa tcctgatcgg gcagatacag aaacggcgcg ctgcttttgc
     4741 ggaactgttt gacgagggcg cgcagccctt cggtgcgccc gataaggaag acgttgtgat
     4801 agcggttgcg gcctttcaaa atctgttcgt ccaatatctt gttttttgat gggaatacat
     4861 actgatcagc gggatatcct gattaagcgc gtacaccgcc atctcgaacg cggtgaagtg
     4921 cggatacagg atgatgactt tttcccccgc cgccagcgcg tcgtccaaat aatgcttatt
     4981 gcggtagcgc accagcgatt tcaaacgtcc ggcaggcgcg taccaatata aaccgtattc
     5041 caacatcagt ttcgccatgt gtttgaaatg ctgtttcaac acggttttac gcttttcctc
     5101 actccattcg gaaaaacatt ttgccaaatt gatttcgccg atacggcggc gcggtttgac
     5161 cagaaggtag gcaagcaaac ccgtcaggtc ggcaatcttg tgcagcagcg caaacggcag
     5221 aaactgcaaa acatacagta caaaaaatat aaatttcatc tcgatacaca ttttcttttc
     5281 agacggcaaa atacaaatgc cgtctgaaac tattgaaacc ttccgcgctt gacctgcatc
     5341 cctgaaggat tgagtttggc ggcaagcccg tggttgcgta aggcgtgggt cagcgcgacg
     5401 gcaagaccgt ccgccgcatc cgcctgcggt gttcccgaaa gccccagcat ctgcaccacc
     5461 atatgctgca cctgttcttt cgctgcctta cccttgccga ccaccgcctg tttgacctgc
     5521 aaggccgtgt attccgaaac gggcagctta tggctgacca atgccgccaa tgccgcgccc
     5581 cgcgcctgac cgagcatcag cgtcgatgca ggattgacgt tgacgaacac ctgttccacc
     5641 gccgcctgtt gaggcttgta aacggtaacg acttcgccga tgtgccggac gatgacggca
     5701 atcctgtctg ccagaggcgc atcggcaggc gttttgatgc agcctgaggc aacgtaaaaa
     5761 tgatcgcgcc ccctgacatc gatgatgccg aaacccgtta cgcggctgcc cgggtcaatg
     5821 cccaagatac ggacggttgc cgacatattc acagcaaacc gtgttgaatc agttttttac
     5881 gcaaggtatt gcggttcagt cccaacatga cggaggcttt ggactgatta ccgccgcatt
     5941 gctccatcac gcacaccagc agcggctttt ccacctgatg caataccata tcgtacacgc
     6001 cgcaaggttc ggtaccgttc aggtctttga aatattgttc taaattttgt ctgatgcatt
     6061 gggaaatatc gggaagggta tggggcatga ttgcactttc aaaggataat caagtgttca
     6121 gaaggcattt gggcggtagg cgcacgccca actgtcggtt ttttcggcaa gtctttcaag
     6181 ataacctgca agcatgtcgt attgcgccgc cgcactgtcc aagcggttga tttcacgacg
     6241 tgtctgttcg ccgtcgggca tttcgtcgat gtaccagcct atgtgtttgc gtgcgatgcg
     6301 cacaccggcg gtgtcgccgt aaaacgcgtg tatggcgcgg atgtggttca aaatagcggc
     6361 ggcgcattct gccaaactca aggcaggcgg caaaacaccg tgttcggcat aatgtttcaa
     6421 atcgcggaag aaccacggcc tgccttgcgc gccgcgccct atcataatgc cgtcggcggc
     6481 ggtttgtttg aggacggctt gggctttttg cggcgaagta atgtcgccgt tgacccagac
     6541 cgggatgttc agacggcatt tggtttcggc gatgagttcg taacgcgctt cgcctttgta
     6601 catttgcgta cgcgtgcgtc cgtggacggc aagggcggcg atgccgcaat cttcggcgat
     6661 tttggcgatg acgggcaggt tttgatggtc gtcgtgccaa cccaaacggg ttttgagggt
     6721 aacgggtacg cctgccgcac ggacgacggc ttccaaaatg gcggcaacca gcggctcgtt
     6781 ctgcatcagc gcgctaccgg cttggacatt gcagactttt ttagcgggac agcccatgtt
     6841 gatgtcgata agctgcgccc caaggctgac gttgtaacgc gcggcatccg ccatctgctg
     6901 cggatcgctt ccggcaatct gcacggcaac aatgccgcct tcatcggcaa aatcgctgcg
     6961 gtgcaaggtt tttctagtat ttctgagcgt cgggtcgctg gtcagcattt cgcacaccgc
     7021 ccaacctgcg ccaaaatctc ggcaaagtcg gcggaacggt ttgtcggtaa tgcccgccat
     7081 cggcgcaagt gcgatggggt tgtcgataaa atagccgccg atgtgcataa tggatccgcg
     7141 tttcaaaaaa gtacgccatt gtacattttt taagcaggat ttccaatctc cggacgcgcc
     7201 cgcgattggg tcggacaccg ttttatggca taatccgcac acagattccc tgccccgcca
     7261 ctcacaggcg ggcagtttat agtggattaa caaaaaccag tacggcgttg cctcgcctta
     7321 gctcaaagag aacgattctc taaggtgctg aagcaccaag tgaatcggtt tcgtgctatt
     7381 tgtactgtct gcggctcgcc gccttgtcct gatttttgtt aatccactat atttccccgt
     7441 cctatcggtt tcccgtttca gacgacataa ggtctgaaag aaagactaca attatgagta
     7501 atccattttc ctctttaggt ttgggtacgg aactcgtttc cgcactgacc gcgcaaggtt
     7561 acgaaaaccc gacgcccatc caagccgccg ccattcccaa agcactcgcc ggtcatgatt
     7621 tgctagccgc cgcgcaaacc ggcacaggca aaaccgccgc ctttatgctg cccagtctgg
     7681 aacgcctcaa acgttacgcc accgccagca cctcgcccgc gatgcacccc gtgcgtatgc
     7741 tcgtcctcac ccccacgcgc gaacttgccg accaaatcga ccaaaacgtg cagggctaca
     7801 tcaaaaacct gccgctgcgc cacaccgtct tgttcggcgg tatgaatatg gacaaacaga
     7861 ccgccgacct gcgtgccggc tgcgaaatcg tcgtcgccac cgtcggacgg ctgctcgacc
     7921 acgtgaaaca gaaaaacatc catttgaaca aagtcgaaat cgtcgttttg gacgaagccg
     7981 accgtatgct ggatatgggt tttatcgacg acatccgcaa aatcatgcag atgctgcccc
     8041 gccaacgcca aaccctgctc ttttccgcca ccttctccgc cccgatacgc aaactggcgc
     8101 aagacttcat gaacgcgccc gaaaccgtcg aagtcgccgc gcaaaacacc accaacgcca
     8161 acgtcgagca gcacatcatc gccgtcgata ccattcagaa gcgcaacctg ctcgaacggc
     8221 tgattgtcga tttgcatatg aaccaggtca tcgtgttctg caaaaccaaa caaagcgtcg
     8281 accgcgtaac gcgcgaactg gtgcgccgca acctgtccgc acaggcgata cacggcgacc
     8341 gttcccaaca aagccggctc gaaacactca acgccttcaa agacggcaac ctgcgcgtcc
     8401 tcgtcgccac cgatatcgcc gcgcgggggc tggatattgc cgaactgccc ttcgtcatca
     8461 attacgaaat gcccgcccag cccgaagact acgtccaccg catcgggcgc acggggcgcg
     8521 cgggcgcgga cggcgtggcg atttccctga tggacgaatc cgaacagaaa atgtttgaat
     8581 ccattaaaga gctgaccggc aacaagctgc tcatcgagcg catcgagggc ttcgagccgc
     8641 aatggtggga acagggcggc gcaaaaccgg aaaaacccga aatgcgcgaa ccaagacaac
     8701 gcaaccgcta cgaatccgcc aaagcgcaac gcgaaaaaaa cacccggccg gaaaatgcgg
     8761 caaacgatgc gggcgcggct tgcggaaaaa ttgccggacg cagccgccga agccgccggg
     8821 aacaccggac gtgcgccctg ctccaaccgc gttacggcgt aaaataaccc tgaaaatcaa
     8881 atgccgtctg aacatttccc gtttcagacg gcatttttca aaccggactg acgcattggg
     8941 cgcaaccgcc cgcaccggat aaatttctgc cgcaaacagt ttcagacggc atttgccgcc
     9001 tgtacaatat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
     9061 atagtacgga accgattcac ttggtgcttc agcaccctag agaatcgttc tctttgagct
     9121 aaggcgaggc aacgccgtac tggtttttgt taatccacta tatcaagtct gcgtgttgga
     9181 aaaacagcac tttccgcgct tcgtcatcgg cgaaagcctg ctgccgcact gtatggaaat
     9241 gctggaagaa gccggatttg ccgatgccgt gcgcgccgag cccggctttc agttgaaaaa
     9301 cggcgcggcg ttttcgtggg gcagccgtta taccgaattc gatttcaccg ataaattttc
     9361 agacggcccc ggcacgactt atcaagtgcg ccgcgccgtg ttcgataaaa tcctgattga
     9421 agaagccgcc aaacaaggcg ttgaagtacg tttcgggcac ggcgtaaccg cgttcgacaa
     9481 cagcggcgat tttgtccgcc tgaacatcga aaccgacacc ggcaagcgct atgaactgac
     9541 cgcgaaattc gtcttggacg caagcggtta cggacgcgtg ctgccgcgcc tgctaaactt
     9601 ggaaacgccc tcgcacctgc cgccgcgcca agcgcatttc acgcacatcg acgacaacat
     9661 caccaacccg aaattcgacc gcaacaaaat cctgattacc acctatccgc aacaccgcga
     9721 cgtgtggatt tggctgattc ccttcggcga caaccgctgt tccgtcggcg tggtcggcac
     9781 gcccgacaaa cttgccggcg aatcggaaac agtgttgaaa aaatttgttt acgaatgccc
     9841 gatgctgaac gaaattttgg acaaagccgt ttgggaaaac gattttccgt tccgctccat
     9901 ccaaggctat tccgccaatg tgaaatcgct gcacggcagg catttcgcgc tattgggcaa
     9961 tgccgccgag ttcctcgatc ccgtgttctc gtcgggcgta accatcgcgc tgcactccgc
    10021 caaacttgcc gccgatctgt tggcaaaaca actcaaaggc gaagccgccg attggcaaac
    10081 cgaatttgcc gaacccttga tgatcggcgt ggacgcgttc cgcacctata gtggattaaa
    10141 tt
//
LOCUS       DAXHTR010000074        10076 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.074, whole genome shotgun sequence.
ACCESSION   DAXHTR010000074 DAXHTR010000000
VERSION     DAXHTR010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10076
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.074"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..410)
                     /locus_tag="ACFNML_001267"
     CDS             complement(<1..410)
                     /locus_tag="ACFNML_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6392647.1"
                     /translation="MKTVIQTELTQALLSHEKVWANEEKTILAKNILLDLVEKTDPTI
                     IGLLLNNDELKRHFFVEVNGVLVFKLQDLRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRS"
     gene            complement(627..1550)
                     /gene="truB"
                     /locus_tag="ACFNML_001268"
     CDS             complement(627..1550)
                     /gene="truB"
                     /locus_tag="ACFNML_001268"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFC6392648.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFAQYLLDADKAYTATLKLGEASSTGDAEGEIVATARAD
                     ISLAEFQTACKALTGDIRQVPPMFSALKHEGKPLYEYARKGIVIERKARYITVYAIDI
                     AEFDAPKAVIDVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLNETERDSLLLSCDVLVSHFPQTVLNDYAVHMLRCGQRPRFEEDLPSGTPVRVYT
                     EIGEFVGLAEYQKEICRLKALRLMNTAASAA"
     gene            complement(1583..1954)
                     /gene="rbfA"
                     /locus_tag="ACFNML_001269"
     CDS             complement(1583..1954)
                     /gene="rbfA"
                     /locus_tag="ACFNML_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFC6392649.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGMNLSALIDQVAAEKPVED"
     gene            2144..3388
                     /gene="clpX"
                     /locus_tag="ACFNML_001270"
     CDS             2144..3388
                     /gene="clpX"
                     /locus_tag="ACFNML_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HFC6392650.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIAWQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGKEPELVFES"
     gene            complement(3453..4646)
                     /locus_tag="ACFNML_001271"
     CDS             complement(3453..4646)
                     /locus_tag="ACFNML_001271"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine/succinyldiaminopimelate
                     transaminase"
                     /protein_id="HFC6392651.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALADALNTQMHKLWHISNIYTTRPAQELAQKLVKNSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGVVPDILSSAKALGCGFPIGAMLATETIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVCSQGQKLQTALRSLGEQTGVFKQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            5050..5550
                     /locus_tag="ACFNML_001272"
     CDS             5050..5550
                     /locus_tag="ACFNML_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392652.1"
                     /translation="MNAMPQTAAQNETLHICSIIVDDARDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5635..6189
                     /locus_tag="ACFNML_001273"
     CDS             5635..6189
                     /locus_tag="ACFNML_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392653.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            6203..6403
                     /locus_tag="ACFNML_001274"
     CDS             6203..6403
                     /locus_tag="ACFNML_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392654.1"
                     /translation="MPSEGLSDGIFAFVRDIVMLPFDFQTAFCFQAFDVGMAILIYVR
                     WQGGIVMPPLGFQTASYRTVKP"
     gene            complement(6678..8066)
                     /locus_tag="ACFNML_001275"
     CDS             complement(6678..8066)
                     /locus_tag="ACFNML_001275"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFC6392655.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEIIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
     gene            8395..9342
                     /locus_tag="ACFNML_001276"
     CDS             8395..9342
                     /locus_tag="ACFNML_001276"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6392656.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAV
                     DVYGDQIHLQEYDTGWLMQPEEYEAWLAEVLEAVAFVTGFAPEQIRLKRRERQKGLQQ
                     YEKTGKAGEDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWARRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRQFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHKS"
     gene            complement(9395..9727)
                     /gene="trxA"
                     /locus_tag="ACFNML_001277"
     CDS             complement(9395..9727)
                     /gene="trxA"
                     /locus_tag="ACFNML_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFC6392657.1"
                     /translation="MSSELIVHTSDAAFEKDVLNADIPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGEVVATKVGALAKGQLT
                     AFVEASIA"
BASE COUNT         2377 a         2620 c         2671 g         2408 t
ORIGIN      
        1 ttactgcgtt taaaataaat ctcttcgcct tcttcggtgc tttgtccgcc attcaatacg
       61 caatctttaa acggaaaatc caacacaata tccgaattgt ctttcaaaaa gcggttgccg
      121 tccgtcaaac caatgcggtt ggcgtatttt gtgtaggaat tattgacgct gtgtttgtcc
      181 aagaaaaaac gtaaatcctg caatttaaac accaaaacac cattcacttc cacaaagaaa
      241 tggcgtttta actcatcatt attcaataac aaaccgataa tggtcgggtc ggttttttcc
      301 accaaatcca acagaatgtt tttggctaaa atggtttttt cttcgttcgc ccatactttt
      361 tcgtgtgata gcagggcttg ggttaattcg gtttgaatga ctgtcttcat cgttataaat
      421 cctcatcagt cattttaatg tgccattctt gtgtaacggc aaatggcttt gcaaataaat
      481 tctaataaaa tcaatccttt gatgtctttt ctgtttcggg gggcgttcgc aaagttgcga
      541 taagcgggga gggtgtccga gtgtgtcggg attttgcgga aatatcaaca cattattcaa
      601 acacagcccg tatttttaac cgtcgttcag gcggcggatg ccgccgtgtt catcaggcgc
      661 aaggctttca gacggcatat ttccttttga tattctgcca aaccgacaaa ttctccaatt
      721 tcagtgtaaa cgcgtaccgg cgtgccggaa ggtaggtctt cctcgaaacg cggacgttgt
      781 ccgcagcgga gcatatggac ggcataatcg tttaaaacgg tttggggaaa gtgtgaaacc
      841 aatacgtcgc aggatagcag caagctgtcg cgttctgttt cgttcaaatt tgccaaggcc
      901 tcaagcgtgt ggctttgggc gatggtaaag ccggcggttt cggtgcggcg caaggcggtc
      961 aggtgggcga acgtgccgat gtgtttggcg atgtcttcgc tgagggtgcg gatgtaggtg
     1021 cctttgctgc aacgtacgtc gatgacggct ttgggcgcgt caaattcggc aatatcgatg
     1081 gcgtaaacgg taatgtagcg cgctttgcgt tcgatgacga tgcctttgcg ggcgtattcg
     1141 tacagcggtt tgccttcgtg cttgagcgcg gaaaacattg gcggcacttg gcggatgtcg
     1201 cccgttaatg ctttgcaggc cgtctgaaat tcggctaagg aaatatcggc acgggcggtg
     1261 gcaacgattt cgccttcggc atcacccgtg ctgctggctt cgccgagttt cagcgtggcg
     1321 gtgtaggctt tgtcggcatc cagcaggtat tgggcgaact tggtcgcttc gccgaagcag
     1381 acgggcaaaa gtccggttgc caaaggatcg agcacgccgg tatgtccggc tttttcggca
     1441 cggaacaaac gccgcgcttt ttgcagcgcg gtgttgctgg aaaggccttc gggtttgtcc
     1501 aagagcaaaa caccgttgac ggcacgtttt tggggtttgc ctgtattcat ggttcgatgt
     1561 tcggacggca tgggcatatc cgtcagtctt caaccggttt ttccgccgct acttggtcga
     1621 taagggcgga caggttcata ccgcgttcca aggattcgtc gtatttgaaa tgcagctcgg
     1681 ggattttgaa cagcttgatg cgtttggcca attcgctgcg gaggtgtccg cgcgcgtgtt
     1741 ccagcacttc ttccgtaatt tcgcgcgcgt cttggtttaa aatggtgtag aacacggtgg
     1801 cgtggctgta atcgcgggta acttcgactt cgttgacggt aatgaagccg gcgcgcgggt
     1861 ctttcagtcc ggtacggacg agttcggcaa gctcgcgcat aatttgttct ttgacacggt
     1921 cttggcgggc atagccgcgt tggggttttc tcatgaatat cctcagattt cagatttggt
     1981 ttcagacggc atttttgccg ccggggcggt tgcgaaaggg cgtattatag ccgaatcggc
     2041 gggattttaa ggtaatacat tgataagaac ggatatagtt attatacttg gcaacccgaa
     2101 cggcgcgtgc cgtttctgta acgcatacat tgtaaagaaa gccatgtcca acgaaaaccg
     2161 tacctgttcc ttttgcggaa aatccaaatc acacgtcaaa catttaattg agggcgaaaa
     2221 cgcctttatc tgtgacgaat gcgtctcaaa ctgtattgaa atattgcatg aagacggaaa
     2281 tgacggcacg ccttcggaaa gtgccggagg ggagccggaa gaatccggca agctccccac
     2341 gcccgccgaa atcgtcgcca acctcaacga ccacgttatc ggtcaggaac aagccaagaa
     2401 agccttggcg gtgtcggtgt acaaccatta caaacgcctg cgccacccga aagccggagc
     2461 taatgtcgaa ttgtcgaaat ccaacatcct gcttatcggc ccgaccggat cgggtaaaac
     2521 cctgctggcg caatctttgg cgcgcaaact ggatgtgccg ttcgtgatgg cagatgcaac
     2581 cacgctgacc gaagcaggtt atgtcggcga agatgtcgag caaatcatca ccaaactctt
     2641 gggcaaatgt gatttcgatg tcgaaaaagc ccaacgcggc atcgtctata tcgacgaaat
     2701 cgataagatt tcacgcaaaa gcgacaatcc gtccatcacg cgtgacgtgt ccggcgaagg
     2761 cgtgcaacaa gccttgctga aactgattga aggtacggtg gccagcgttc cgccccaagg
     2821 cggccgcaag catccgaatc aggaatttat caacgtcgat accaccaaca tcctgtttat
     2881 ctgcggcggc gcgtttgcag gcttggaaaa agtcatccgc cagcgtactg aaaaaggcgg
     2941 tatcggtttc ggtgcgtccg ttcacagcaa ggacgaaaat gccgacatta ccaagctgtt
     3001 cggcatcgtc gaaccggaag atttaatcaa attcggtctg attccggaat taatcggacg
     3061 tttgcctgtg attgcaacgt tagaagaact ggatgaggac gcgctgatta atattttgac
     3121 cgagccgaaa aacgctttgg tcaagcagta tcaagccttg ttcggtatgg aaaatgtcga
     3181 gttggaattt gaagaaggcg cattgcgttc cattgcgtgg caggcaatgg agcgcaaaac
     3241 cggcgcgcgc ggcctgcgtt ccatcgtcga acgctgcctg ctcgatacga tgtaccgcct
     3301 gcctgatttg caaggcttga aaaaagtggt ggtcggtaag gcagtcatcg aagagggcaa
     3361 ggaaccggaa ttggtgttcg agtcctgatg gcaaacaaaa ccgcttcgga acgttgaacc
     3421 acgtttccga agcggttttt tgtttttgat gcctaggggc ggtttgcggc ggcaaattcc
     3481 gtcagcacgt gttccaaacg ccgcaaacct tccgccgtat cctcatcgtc caacagcagc
     3541 gaaggcgcga aacgcaatac gtccgcaccc gcaaccaaaa tcatcacgcc gtgtttcaag
     3601 gcggcggcgg ttatttcgga tgcgcgtccg cgataaggcg cgtccaacac gcagccgagc
     3661 agcagcccca tgccgcgaac ttgcttaaac acgcccgttt gttctcccaa agaccgcaat
     3721 gccgtctgaa gtttctgccc ctgactgcag acgttgtgca aggtttccgg cgcgttgatg
     3781 atgtcgaagg cgcggctgcc gaccgcacac gccatcgggt tgccgccgaa agtcgagcca
     3841 tgcgtgcccg gttggaaggc ggcggcaatc gtttctgtcg ccagcatcgc gccgatcgga
     3901 aagccgcagc ccaaggcttt tgccgaactc aaaatatcgg gaacaacgcc gtaatgttca
     3961 taggcaaaca gcctgcccgt atgccccatc ccggtttgaa cttcgtccaa aatcaacaac
     4021 gcattgtgcc ggtcgcacag acggcgcgcg gtttgcaaat attccgcagt ggcgggcagg
     4081 atgccgcttt cgccctgtat cggctcgatg atgaccgcgc aggtctgttc gccgacggca
     4141 gcttccagcg cggcaatatc gttgaacgga acgtgcgtaa tgccttgcgg caggggtgca
     4201 taatccttgc tgtatttcgg ctgaccgccg acggacacgg taaacagcgt gcgtccgtgg
     4261 aaactgttga tacaggcgac gatttcgctt tttcctccgc cgaaacggtc gcgcgcatat
     4321 ttccttgcca gcttcaacgc cgcctcattc gcttccgcgc ccgagttgca gaaaaaaacc
     4381 ttgtcggcaa aactgttctt caccaatttt tgcgccaatt cctgcgccgg acgcgtcgta
     4441 tagatattgg aaatgtgcca cagcttgtgc atctgcgtgt ttaaagcatc ggcaagggca
     4501 gggtggcagt gtcccagcgc attgacggcg atgccgcctg aaaagtcaat atattcacgc
     4561 ccttccgtat cccaaacgcg gctgcctgaa gcgcgttcgg gaatcatcgg tgcaaaggcg
     4621 aaattggggg tcagatagtt ttgcataatt gctcctgatt gcggttttgc tcgggattat
     4681 gcaccgtttt tcaaagcaga taaagggaaa ttgccgacaa tatcgggcag cggtaaaaat
     4741 aaaccgaaat attttcaatc ggatagatga aaaatcaaaa aaatttcgat aaaatgcttg
     4801 aggcgcggca gcgttttgat ttagaatctc cccaatattt ttatcttcag acggcatttc
     4861 atcatgcaag gcaaaaaatt tacaaccgct tattgctatt ggtaccacac agacgtgcgg
     4921 tacttttttg cgcttgcttg aaatccatcc catacgcaaa acaggccgct cgaaaatggg
     4981 gcggcttttt tgttaccttc acacaggtcg cccgattcgg caacacacga cagaatcagg
     5041 aaaatgacca tgaatgcaat gccccaaacc gccgcacaaa acgaaaccct gcacatttgc
     5101 agcattattg tcgatgatgc acgggatttt tcccccatgc ttctcgatgc agggcgggac
     5161 gacacactca tcagcgaatc gatgattccg caaatccgca ctgttgccgc cctgatagcc
     5221 gccgaacggc acgacttcag ccgcagcagc cccgccgagt ttaccgatgc cgccgatttt
     5281 tttgccgcac ggattctcgt attgggcgtg cgacgcttcc atctggacgt atcgctgctt
     5341 cagattttga aaaccgccaa cagccgcgcc cgccgctttg ccgaaaaaca ccgcctgttt
     5401 ttcacacccg cacaagccga attgagcctg aacaaacaca aaaaccgccg cctgctgacc
     5461 gtagaaaccg aacacgaagt cgaaaacaaa ggcaacccag tcgccaacag cctcgcattt
     5521 gtccgcaaat tgcacaccct gcccctgtaa acatcacgat ttgccggcag aatgcttgcc
     5581 aaaattcccg acggcgatac aataaaaccg attttccacc caaacaggac acacatgaaa
     5641 aaaatcatcg cctccgcgct tatcgcaaca ttcgcactcg ccgcctgcca agacgacacg
     5701 caggcgcggc tcgaacagca gcagaaacag attgaagccc tgcaacagca gctcgcacag
     5761 caggcagacg atacggttta ccaactgact cccgaagcag tcaaagacac cattcctgcc
     5821 gaagcacagg caaacggcaa caacgggcaa cccgttaccg gtaaagacgg gcagcagtat
     5881 atttacgacc aatcgacagg aagctggctg ctgcaaagcc tggtcggcgc ggcggcaggc
     5941 gcgtttatcg gcaacgcgct ggcaaacaaa ttcacacggg caggcaacca agacagtccc
     6001 gtcgcccgac gcgcgcgtgc agcctaccat cagtccgcac atcccaatgc gcgcaccagc
     6061 agggatttga acacgcgcag cctccgtgca aaacaacagg cggcgcaggc gcagcgttac
     6121 cgcccgacaa cgcgcccgcc cgccaattac cgccgccccg ctatgcgcgg tttcggcagg
     6181 aggcggtaaa cccggcgtgc caatgccgtc tgaagggctt tcagacggca tttttgcatt
     6241 tgttagggac attgttatgt tgccgtttga ttttcagacg gcattttgtt tccaagcgtt
     6301 tgatgtcggg atggcaattc tgatttacgt gcgttggcag ggtggcattg tcatgccgcc
     6361 gttgggtttt cagacggcat catatcgaac agtcaagccg tagggcgagc attcttgccc
     6421 accgttgcaa gggcgggatt gggtgggcag gaatgcccac cctgcatctg aaacccttcc
     6481 gtgtcattcc cgtgcaggaa ggaatctaga tatttgaatt tacggaaatt ttcaatcgtt
     6541 gccaaagcgg aaaagggttt ttattacagc ctaatccttt gggtatcaaa gccgggcggt
     6601 gttgaaacat tgcaaaaatg ccgtctgaaa cccgaaatcg gtttcagacg gcattggttt
     6661 atgcggcagg ttggggctta gcggtctttg taacggcgtt cgcctctgcc tgcaaaagaa
     6721 tcgccggctt tacgtttgcc tttgaagcct tcgccggttt ttttgaaacc gtctttctta
     6781 aagccttctt tcttgaaacc ttcgccgcgc gttttgccgc cgaagccttc tttgcccggt
     6841 ttatgatcgc cgcgccggcc gccggatttc ctatcgcccc agccgccttt gccttttggc
     6901 ttaccgcctg cggatttgcg tttgcgggtc ggttccatgc cttcgatggt cagttcgggc
     6961 agtttgcggt taatgtattt ttcgattttg tggactttga cgtattcgtt cacttcggca
     7021 aacgtaatcg caatacccgt gcggcctgcg cggccggtgc gcccgatgcg gtggacgtag
     7081 tcttccgcct gtttcggcag gtcgtagttg atgacgtggg taatggtcgg tacgtcgata
     7141 ccgcgtgcgg caacgtcggt ggcaaccaaa attttgcagc ggcctttgcg taaatccatc
     7201 agcgtgcggt tgcgccagcc ctgcggcatg tcgccgtgca ggcagttggc ggcgaaacct
     7261 ttttcgtaca attcatccgc gatgacttcg gtcatcgctt tggtggacgt gaaaatcacg
     7321 cattgatcga tgttggcatc acgcaggatg tggtcgagca ggcggttttt gtgacgcata
     7381 tcgtcgcagt acagcagttg ttcttcgatt ttgccttggt cgtccacgcg ttcgacttcg
     7441 atgatttcag ggtctttggt cagtttgcgc gccagtttgc cgaccgcgcc gtcccaagtg
     7501 gcggagaaca acaaagtctg acggtcgctc ggcgttgctt ccacgatggt ttcgatgtcg
     7561 tcgataaagc ccatgtctaa catgcggtcg gcttcgtcca aaatcagcac ttccaaacgt
     7621 tcaaaatcaa ctttgccgct ttgcatcagg tccatcagac ggcccggcgt ggcgacaatc
     7681 agatcgaccg gtttgctcag ggcgcgggtt tggtagccga acgatgcgcc gccgacgatg
     7741 ctgacggtac ggaaccaacg catatttttg gcatacgcca gcgcgttttt ctcgacttga
     7801 gccgccaatt cgcgggtcgg ggtcaacacc aaagcacgcg ggcctttgcc cggtttttcg
     7861 ctgcgtttgg tcagtttttg caaagtcggt aacagaaagg cggcggtttt gccggagccg
     7921 gtttgcgccg aagccatgat gtcgcggcct tccaaagcaa acggaatcgc ctgcgcctga
     7981 atcggcgtcg ggctttcgta accctcgctg ctgacggcgg acaagatgtt tttatcgagg
     8041 ttcaaatcgg caaatttaat agacatggct atccttggaa gacaacaacg cccgcgtccg
     8101 aaaggttcag acggcctgaa gcagggaagg gtaacgatac ggggaatgtg aagttacagg
     8161 gttgtcgcaa gcggcttgct tgtggtttac atcgacgaca acctgcacag aaaggcttgg
     8221 caaatatgca agcaacaaaa gaaacgcgcc cgtaagggcg gtttaggttg gaaggcgatg
     8281 gcttcgtatg acaagataag cggtggatgg tatagggttt gcccgaaacg gtcaagcaat
     8341 cgggcgttaa ttacagtaaa attgccgttt tccactttat tccgcctgaa accaatgacc
     8401 gacatcacac cgtttgccaa ccgcttgggc aaaaacatca aacaccttat gaaatgggcg
     8461 aaacgcaacg gcatcgaagc ctggcgcatt tacgaccgcg acatcccaca attccccttc
     8521 gccgtcgatg tttacggcga ccaaatccac cttcaggaat acgataccgg ctggctgatg
     8581 cagcccgaag aatacgaagc gtggcttgcc gaagtcttgg aagccgtcgc cttcgttacc
     8641 ggttttgcgc ccgaacaaat ccgcctcaaa cgccgcgaac gccaaaaagg tttgcagcag
     8701 tacgagaaaa ccggcaaggc gggcgaagat ttcgtcatca ccgaaaacgg ccgcaagttt
     8761 tgggttaacc ttgataagta tttggatacg gggctttttt tggatcatcg aaacacgcgc
     8821 aaaaaagtcg gcgaaacggc ggcgggcaaa cgttttttaa acctgttttc ctacactggc
     8881 agcttcaccg tctatgccgc caccggcggc gcggcatcca gcgaaactgt cgatttgtcg
     8941 aacacttatc tcgattgggc gagacgcaat tttgaattga acggcatcga tacggaacgg
     9001 cacaaaatcg tccgcgccga cgtgttccaa tatcttcaga cggcatatgg ggaaggcagg
     9061 cagttcgacc tcattgtgat ggatccgccc agtttttcca acagcaagaa aatgtccgac
     9121 atcctcgaca ttcagcgcga tcacaaaaag ctgattgacg gcgcggtgaa gctgctcgct
     9181 tcagacggca ttttgtactt ttccaacaac ctgcgcagct tcgtcttaga cgacttggta
     9241 tcggaacaat acgccgtcaa agacatttcc aaacaatccg ttcccgaaga tttccgcaac
     9301 aaaaaaatcc ataggtgctg ggaaatccgc cacaaatctt aagggtgcga aaaagccgcc
     9361 ggaatcttcc ggcggctttt tcgattgcgc tttatcaggc gatagaggct tcgacaaagg
     9421 cggtcagctg accttttgcc aatgcgccga ctttggtggc gacgacttcg ccgtttttga
     9481 acaccatcag ggtcggaatg ccgcgcacgc cgaaacggga cggggtggct tcgttgtcgt
     9541 cgatgttgat tttgaccact ttcagacggc cttcaaattc ggcggcaatg tcgtccaaaa
     9601 tcggggcaat cattttgcag gggccgcacc acggagccca aaagtccagc aggacgggga
     9661 tatctgcatt taaaacgtct ttttcaaaag cggcatcgct ggtgtgtaca attaattcgc
     9721 tgctcattat ttttcctttt cggttgggat tggcacgata gcgtgcagga tagggcttgg
     9781 gcataaaaat ttcaagtggg cgggcgggat aatagttttt ctgtaccgcc ccaatcgcct
     9841 ttgcaaattc taaaaaaacg gttcggacag tattttattt ctgaaaatta cttttattat
     9901 caaaatgtaa ggaatgtttt tgaagctgta cagaaacgcg cgggcggtgt atgctccgtt
     9961 taaatagatt cttaaagtat gaatgttcaa aaccaaaccc gaaaccctat aaaaggaaca
    10021 ctgcgatgac tgatttttcc gttgggaaac cgcccccttt ggcgcaaccg tcgatc
//
LOCUS       DAXHTR010000075        10031 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.075, whole genome shotgun sequence.
ACCESSION   DAXHTR010000075 DAXHTR010000000
VERSION     DAXHTR010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.075"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(597..830)
                     /locus_tag="ACFNML_001278"
     CDS             complement(597..830)
                     /locus_tag="ACFNML_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HFC6392658.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(996..1196)
                     /locus_tag="ACFNML_001279"
     CDS             complement(996..1196)
                     /locus_tag="ACFNML_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFC6392659.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(1368..2243)
                     /gene="xerD"
                     /locus_tag="ACFNML_001280"
     CDS             complement(1368..2243)
                     /gene="xerD"
                     /locus_tag="ACFNML_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFC6392660.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRT
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTCLLKPPKIDK
                     NIPTLITEQQISRLLAAPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGNVDLDRG
                     CITALGKGDKQRMVPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(2295..2735)
                     /locus_tag="ACFNML_001281"
     CDS             complement(2295..2735)
                     /locus_tag="ACFNML_001281"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HFC6392661.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            3056..3994
                     /locus_tag="ACFNML_001282"
     CDS             3056..3994
                     /locus_tag="ACFNML_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HFC6392662.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKELLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            4123..4416
                     /gene="hfq"
                     /locus_tag="ACFNML_001283"
     CDS             4123..4416
                     /gene="hfq"
                     /locus_tag="ACFNML_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFC6392663.1"
                     /translation="MTAKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            4501..5067
                     /locus_tag="ACFNML_001284"
     CDS             4501..5067
                     /locus_tag="ACFNML_001284"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6392664.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            5121..5495
                     /locus_tag="ACFNML_001285"
     CDS             5121..5495
                     /locus_tag="ACFNML_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HFC6392665.1"
                     /translation="MQYLIVKYSHQIFVTVTILLFNIRFFLLWKNPEKPLAGLWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSVKFYAV
                     YLLAICCVACIVYLAKTKVLPF"
     gene            5505..5999
                     /gene="folK"
                     /locus_tag="ACFNML_001286"
     CDS             5505..5999
                     /gene="folK"
                     /locus_tag="ACFNML_001286"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFC6392666.1"
                     /translation="MNNRHFAVIALGSNLDNPAQQIRGALDALSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTDMDGISLLSLLNRIEADFGRERSFRNAPRTLDLDII
                     DFDGLSSDDPRLTLPHPRAHERSFVIRPLAEILPDFVLGKHGKVAELSKQLGNQGIRL
                     LPDK"
     gene            complement(6126..6605)
                     /locus_tag="ACFNML_001287"
     CDS             complement(6126..6605)
                     /locus_tag="ACFNML_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HFC6392667.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(6825..7562)
                     /gene="ubiE"
                     /locus_tag="ACFNML_001288"
     CDS             complement(6825..7562)
                     /gene="ubiE"
                     /locus_tag="ACFNML_001288"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFC6392668.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDAALKEMYRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDADSYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(7605..7973)
                     /locus_tag="ACFNML_001289"
     CDS             complement(7605..7973)
                     /locus_tag="ACFNML_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HFC6392669.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKQSDC"
     gene            8093..9712
                     /gene="mnmC"
                     /locus_tag="ACFNML_001290"
     CDS             8093..9712
                     /gene="mnmC"
                     /locus_tag="ACFNML_001290"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016645 - oxidoreductase activity, acting
                     on the CH-NH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFC6392670.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASHGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHDTEQTELLLAGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITPAEAEKIAGIPLNTPYAEPLCGLFWQYGVW
                     LNPPAFVRTLLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSPR
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAHLNPALSESLFAANPNPQKHQGHAAIRCDSPDHLPLVGAL
                     GDIAAMRQTYTKLALDKNYRIDTPCPYLPNAYVNTAHGTRGLATAPICAAAIAAQILG
                     LPHPFSQRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(9826..9943)
                     /locus_tag="ACFNML_001291"
                     /pseudo
     CDS             complement(9826..9943)
                     /locus_tag="ACFNML_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain protein"
BASE COUNT         2683 a         3050 c         2014 g         2284 t
ORIGIN      
        1 tgctcgccag cctactaatg aaaaactgaa ataacaaggg gtggcaaatt accgcgcccc
       61 tttcgccaac ctttcttcga gccattttac tacaatggtt ttaaaaatca aggattgcct
      121 gatgccgccc gcccctctgt ttcctccgtc aagcaccgcc ttggcggttt tcttatatag
      181 tggattaaca aaaaccagta ctgcgttgcc tcgccttgcc gtactatttg tactgtctgc
      241 ggcttcgtcg ccttgtcctg atttttgtta atccactata aaatctaccg attacacaaa
      301 cacatatcat ctttacacaa tcatgcttcc atcaacagta aaacatgata tgattgccaa
      361 caataacatc tcacaataaa ttttctaatt tttattgaaa aaatcaataa ataagaattc
      421 tcccaaccga caaataaaat aaagaaaggg ttaatatgca acatcgaaga agactagcaa
      481 tttaccaagc atccaaacgt gcttccttta ccggcaggtc atcagccccg caaaaacgta
      541 aagaacgtta atttgaaaaa aatgccgtct gaagtcctgc ttcagacggc atttttttac
      601 cgttcgagaa actgtttcaa cctgtcctca tccaaaaacc agtggcagat ttcctcatct
      661 cccgccaaca aaacgggaac cagctcattg tatttttctt ccaaaacagg attttcatcc
      721 acatcgacca cttccagccc gaacccgtat tcattttgaa aaggtttgag tgcatcgcgc
      781 attttgtggc acaagctgca atattcacga aacatcaagg tcaatttcat ccgcgtttcc
      841 ttatctgtca atttgcacac gccaaagcct tagacgcagc agaatcatgg tctatttggg
      901 aaaaaacaat gttttcaagg aagatgatac tcaagtcctg ccaaaaacag caaaaatgcc
      961 gtctgaacag ttcagacggc atttccaaaa ccgttttacg cttgaacatt gatacccgcc
     1021 gtaaccgtcg gttgcgcatt gtgcgccgtc agaaacgaag cggcaagctc cccacagcag
     1081 ccgcagcagc tcgctacgcc caattgcgac tgcaaatcgc ccattgtggt cgcgccggcg
     1141 gcgatggttt ccttgatttg atggtcggta acggcattgc agatgcagac aaacattttg
     1201 tgctccgtgt gttctcaaac tatcgtaccg atagcggctt ttattatcgt atgcgaatat
     1261 aaataaaaac ggttcgcatt gcaaggtcgg tatacacggt ttgtcttggt aattttttat
     1321 caagtttgca ttttcgattg atttcttatt aaaccaaagt agaagcctca gtttcgggaa
     1381 tggtgttcct tcactacacc ctgcaaccat acgttggcaa catgggtata aatctgcgtc
     1441 gtattcaaat cggcatgtcc caacatatcc tgaaccacgc gcaaatccaa gccgtgccgc
     1501 accagatgcg tggcaaaggc gtggcgcagg ctgtgcgggc tgatgtgccc gatgcctgcc
     1561 tgacttgcat attctttgac aatcatccat gccaactgac gggaaatgcc cgtctttttc
     1621 tgactgacaa acaatgcgtc gcaattcctg cctttcagca gaagtgggcg tgcctccgta
     1681 taatagcgtt ccacccaata cgccgactcc tgccccatcg ggaccatcct ctgcttatca
     1741 ccctttccca gcgcggtaat acagcccctg tccaaatcca cattgccgaa gttcagcccg
     1801 accgcctcgc tgacgcgcaa gccggtcgcg tacatcaatt cgagcaaagc cttatcccgc
     1861 aaaccgtgcg gcgtgtcggt atccggggcg gcaagcagtc gggaaatctg ctgctcggtg
     1921 atcagggtcg gaatattctt gtcgattttg ggcggtttca gcaggcaggt gggattgtcc
     1981 gtccttatgc cttcacgctc catccatata tacaggcgtt tgcacgccga taatgcgcgc
     2041 gcctgcgaac tccgttgctc tccgtcaaca taaaccgccg ccgccaaatc cgcttcgtcc
     2101 gcatccttca gcgttctgcc cgattgggac aggcggcggg cgattttttc caaatcgcgc
     2161 cggtaaccgt ttaaagtatt ctgactgagc cgcctgtcca accacagcgt ttcaagcagc
     2221 ctgtcgatca aaccttcttc cataccgttc caaacaaatg ccgtctgaat cttcttcaga
     2281 cggcatggtt cacattatcg ggaaagcgtt tccaatactt cctgcgcgtg acccgccact
     2341 ttgactttcc gccattcatg ggcgatttct ccatccttat tcaagacgaa cgtactgcgc
     2401 tcgataccta acgactcttt cccgtacagt ttcttcaatt tgatgacatc aaacaggcgg
     2461 cacactgttt catccttgtc gctcaacagc tcgaaccgga aaccctgctt ggcgcaaaaa
     2521 ttctgatgcg cctttacgcc gtcgcgggaa ataccgacca cggtataacc caatgcctca
     2581 aactgttcca aacgcgcatt gaaatccaag ccttccgtcg tacagccagg cgtactgtct
     2641 ttcggataaa aatacacgac caaaggcaga tgttctgccg aatgaaaatc cgcaccgctg
     2701 ctcgaaggca gggtaaattc atatttcaca tccatagtcc tactcccgat attcccatta
     2761 ttcaaaacgg cacgcagacg caccgccgca attgccaaac caaccccgat tctaccgccc
     2821 caaaggacaa ggattcaacc gccggaaaca tccaaaccga cacacgacgg catgaaaaat
     2881 atccatgtca aaccacaaaa tatgttccga tttaaaaaca gaatgttata aaacccaatc
     2941 cccaaaacac aacaagaccg cccgcctacg ggcagtctcc tgtcagacga catactttac
     3001 agatggctgt tttttcaaca aaataacgcc aatactcaaa aatatggaat caaaaatgtc
     3061 catccatact ctgaaacgcc tgccctcatc gctgctgctc ggtctctgcc tttccctgcc
     3121 gtcagcccac ctttttgccg acaacgacat tttagggcaa tttttagaac agaacatgct
     3181 tacctcctcc gatccgatag aaatattcgc cgaaagcaca atacacccca ccaacaccca
     3241 agccattaca ggcggtctga ttctctcctc acagtctgcc ctggtcgtca acaacaaaac
     3301 cggacagata ctgtatcaga aaaacgccga caggattatg cccatcgcct ccatttccaa
     3361 actgatgagc gcgatggtcg ttttggatgc aaacttggac atgaacgaaa ccgttaccat
     3421 tacgcccgac gaaatcgacc gcatcaaagg gaccggcagc cgtcttgcca taggtacggc
     3481 acttacacgc aaagaactgc tgcacctgag cctgatgagc agcgaaaacc gcgccaccca
     3541 tgcattgggc agaacctacc ccggcggcat gggcgcattt gtcgccgcca tgaaccgcaa
     3601 agcccaaagc ctcggtatgt acggcagccg cttttacgaa ccgaccggac tcaacttcca
     3661 aaacgtttcc accgccaaag acctgagcct tatggtcaac gccgccgccc aatatccgca
     3721 aatccgcacc aactcgactt ccaactacgc ctcggtacag accaaaaacg ggcagcagaa
     3781 ctacaaaaac tccaatgccc tggtcagaga aggcatgtgg aacatcgaat tgcagaaaac
     3841 cggctacata cgcgaagcag gcaggtctat ggttgtcaaa gccaacattc aaaatcaacc
     3901 cgttaccatc gtattgctga actcgcccac atccgccaca cgcgtcaacg acgcccgcaa
     3961 aatcgaatcg tggatgctgc agcaacgctc ctgacataca aatgcccggc ggaaaaccgc
     4021 ccaaacacaa aaaaaattgg taatttttcc tattcaaggc tacaattcga cacgcacagg
     4081 gcgtctgccc gatttcacaa tcataataac ggagttaaaa atatgacagc taaaggacaa
     4141 atgttgcaag atcccttcct gaacgccctg cgtaaagagc acgttccggt ttcgatttac
     4201 ttagttaacg gtatcaaatt acaaggtcag gttgagtctt tcgatcaata cgttgttctc
     4261 ctgagaaaca cttccgtcac ccaaatggtt tacaaacacg ccatttccac catcgtaccg
     4321 gcacgctccg tcaacctaca acatgaaaac agaccccaag ccgcaccgac ttcgaccctc
     4381 gtccaagtgg aaaccgtcca gcagcctgcc gaataatccg cacgaagcat gacgtgtcat
     4441 atctttcaat accttaccgg acaacggtaa ggtattttta ttttcagaca gcatttaaaa
     4501 atgttattgc aaaacatcct tccattcgcc cattgccttt tgcggaaggc acttcccgaa
     4561 ggtggcaatg ctttggacgg caccgccggc aacggacacg acaccctttt cctcgcacaa
     4621 accgcaggca tccgggggaa agtgtgggca ttcgacatcc agccgcaagc cctgagccgc
     4681 actcgatgcc gtctgaaaga agcgagatac gagaatgtgc aactcatctt agacggacac
     4741 gaaaacctga aacaatatgt ttcagaacca ctaaatgccg ccattttcaa ctttggctgg
     4801 ctgcccggcg gagataaaag ccttaccacc catacggaaa ccagcattcc cgccctttgc
     4861 gcaaccctct ccctgctgaa agaaggcggt atgcttattg ccgtcctcta tccgggacac
     4921 gaaaacggca aacaggaggc agaagcaatc gaacaatggg caaaaaacct gcctcaagaa
     4981 cagtttgccg ttttgcgtta cggctttacc aaccggaaaa acaacccgcc ctatctgttg
     5041 gcatttgaaa agttacgtca aaaataaatg tttgcggtaa aataagccga ttccaaccct
     5101 tattttgata agagccgact atgcagtatc tgattgtcaa atacagccac caaatcttcg
     5161 ttaccgttac cattttgttg ttcaacatcc gctttttcct gctttggaaa aatcccgaaa
     5221 aacctttggc gggcttgtgg aaggcactgc cccacctcaa cgacacgatg ctgctgttta
     5281 cgggattgtg gctgatgaaa atcacccatt tctccccgtt caacgcgcct tggctcggca
     5341 caaaaatcct gcttctgctc gcctatatcg cattgggtat gatgatgatg cgcgcccgcc
     5401 cccgctcggt caaattctat gccgtttacc tgcttgccat ttgttgcgtc gcctgtatcg
     5461 tttaccttgc caaaaccaaa gtcctgccat tctgaaacac tgccatgaac aacagacatt
     5521 ttgccgtcat cgccttgggc agcaaccttg acaaccccgc acaacaaata cgcggcgcat
     5581 tggacgcgct ctcgtcccac cccgacatcc ggcttgaaca ggcttcctca ctgtatatga
     5641 ccgcgcccgt cggttacgac aatcagcccg atttcgtcaa tgccgtctgc accgtctcca
     5701 ccgatatgga cggcatctcg ctgctctctt tgctcaaccg tatcgaggcg gatttcgggc
     5761 gcgaacgcag tttccgaaac gcgccgcgca cattggattt ggacatcatc gactttgacg
     5821 gcctatccag cgacgacccc cgcctcaccc tgccgcatcc gcgcgcacac gaacgcagtt
     5881 tcgtcatacg ccctttggca gaaatcctcc ctgattttgt tttaggaaaa cacggaaaag
     5941 ttgccgaatt gtcaaaacaa ctgggcaatc aaggtatccg tcttttaccg gataagtaat
     6001 tccgcacgcg gatgccgtct gaatgccggt ttcagacggc atttttcctt tgccgccaac
     6061 acgcgtgcaa aaaaatcgcc ccttggaaaa ggggcgcaaa aggaacacaa accactacca
     6121 aaactttaaa tctgcaacat tgcctgccgc atactgtatc cgacaggata taaagccctc
     6181 actaaatcgt ttcgagaaat ccaaacttct tcatcgccga cagaaaatct gcctttctcc
     6241 ggcaccagct ccaacaaaaa cggttgaacc gccgtatgca gcctgtcctt acaccgcccc
     6301 agctttctgg acaaggcgga cagggcacgc ttatagacac tatccttgcg gtcaagttcc
     6361 cgcgcactgc cgacccagct caaacgaagg ttgcggcttt catcctcaat cagcaaaatg
     6421 cctgttttga cttcccccac ctctggctcg catgaaagcg aaatataata tttgccgtcg
     6481 atgttgacat acgccgcctc atgcacctga cagttgcagc cggaagtggc aagcagcgaa
     6541 taaacagaat cccgatgcag tttcaaaata tcaagggaaa tctgtttgaa aatactttcc
     6601 ggcatttttc cctcctttcg tctgctgcgt tgtttactgg attgactgca ttatacagac
     6661 atctcccaat cttgcaaatg ataattgttc ctatttttta aaaaatttat aaattattga
     6721 tagatatgtg aattaatttt aagatttaaa atctctttct caaaaaaaca caaaaatgcc
     6781 gtctgaaacg tgttcagacg gcattggctg cacaggcaat ccgtttaaaa tttcacgccc
     6841 ttatgcagcg caacgatccc cgcactcata ttatgatagt ccacactgtc aaatccggca
     6901 tccagcatca tttgctttaa agtttcctga tcggggtgca tacggatgga ttcggcgaga
     6961 tactggtagc tgtccgcatc tttggcaatc agtttgccca tgaccggcag cagcttgaac
     7021 gaatagaaat catacgcgcc ttcgagcggc ttgtagattt tggaaaactc caacaccagc
     7081 aacgtgccgc caggtttcaa aacacgatac atctctttca gcgcggcatc tttatgcgtc
     7141 atgttccgca agccgaacgc cacggaaact aagttgaaat aattgtcagg gaaaggcagt
     7201 ttttccgcat cggcaagcga cacgggcaaa atcatgcctt cgttcaacag acggtcgcgc
     7261 ccgacggtca gcatagagga attaatatcg gtcagccaaa cctcgccttc cttgccgacc
     7321 cgtttcgccc aaccgcgcga caaatcgccc gtaccgcccg caatgtccaa cactttatcg
     7381 ccttttttca ggtgcgccgt gttgatggtg aaatgcttcc acaccctgtg cagccctgcc
     7441 gacatgacat cattcataat gtcgtagttt ttggcgacgg agtggaacac ttccgccact
     7501 ttgccggctt tttcatcttc gttgaccgtg ctaaacccga aatgcgtttt ttgtccgccc
     7561 atattctaac cacctccctg ataccgccct acggcatccc aaacttaaca atctgattgc
     7621 ttttcaaaac gcgacgcgcc cgccgccgcc aatttgtcca aatattcctg ccacatcgca
     7681 tcgtatccgt atgccagtct gtgcagatac gcccaatcgt aaagaccgct gtcgtgcccg
     7741 tccgaaaaac tgattttcag cgcgtactgt ccgacaggct gcaaatccgt aatttgcaca
     7801 tctgccttac cggtctgcaa aacatcctgt cccacaccgt gtccgcgcac ttccgcactc
     7861 ggcgaataga cgcgtaaaaa ttcggcaggc agatttttag gttcgtcccc ataagtcaaa
     7921 accaacacgg tacggttttt caacaaacgg atttcaacag gaatattatt caaaaaacac
     7981 ctcggttcgg catacaatat ccggcaggcg caggcgtgcc ttcaaacggc gcgtatttta
     8041 acagctttat cccgataccg atattcaaac ccacacttta aacggcattg atatggacaa
     8101 tctcgtatgg gacggcgttc ccgacatccg cacactcgac caaaccatcc gcaaacacgc
     8161 acacccgctc aacctgattg tctgcctccc cgataatcag attcccgatt ttcaaaccgc
     8221 acaagatgct tcggacgcgg aatgccgtct gaagcaccgt ttggatcagg caacgcagtg
     8281 cctccagttc gacagcatca acctcatcga acacatcctg cccgatgtcc gcttctggct
     8341 ggttccccct tcacgcaccc gccgcctgca cgaacacttc caccacattt cctggcagac
     8401 cgaagccatc ccgcaaaccg aaagcaaacc cgacaaaccc tggtttgcac ttccacaaac
     8461 atccgaacgg caaaaaccgg aacacatcct cgttatcggc gcgggcatat ccggcgcggc
     8521 aaccgcccac gccttagcat cacacggcat ttccgttacc gtattggaag cccgaaaagc
     8581 cgcccaagcc gccagcggca accgccaagg gctgctctac gccaaaatct cgccgcacga
     8641 caccgaacaa accgaactgc tgcttgccgg ctacggctac accaaacgcc tgctcggaca
     8701 catcctgccc gaatccgaaa cctggggcgg caacggcatc atccacctca attacagccg
     8761 caccgaacaa caacgcaatc acgaattggg tttgcaaaaa caccataacc acctctaccg
     8821 cagcatcacg ccggcagaag ccgaaaaaat cgccggcatc ccgctgaaca cgccctacgc
     8881 cgaaccatta tgcggactgt tttggcagta cggcgtatgg ctcaatccac ccgcattcgt
     8941 ccgcaccctc ctcaaccatc cgctcattgg actacacgaa gacacaccgt taaccgacat
     9001 ttcccacgac ggggaaaagt ggattgcaag cacgccaaac ggcatatttt ccgccacaca
     9061 catcatctac tgcaccggcg cgcacagccc tcgcctgccc gaaaccaacc tcgccgccct
     9121 gcctctcagg caaatacgcg gacaaaccgg cctcacaccg tccaccccgt tttccgaaca
     9181 actgcgttgc gccgtttcag gcgaaagcta catcagcccg tcgtggcacg gactgcactg
     9241 ctacggcgcg agttttattc ccaacagcag ccataccgga tggaacgaag ccgaagaagc
     9301 ctcaaaccgc caagcattgg cacaccttaa ccccgccctt tccgaatcat tgtttgccgc
     9361 caacccaaac ccccaaaaac accaagggca cgccgccata cgctgcgaca gccccgacca
     9421 ccttccccta gtcggcgcac tcggcgacat tgccgccatg cggcagacct acaccaaact
     9481 cgcgctggac aaaaactacc gcatcgacac cccatgccca tacctgccta atgcctacgt
     9541 caacaccgcg cacggcaccc gcggactcgc caccgccccc atctgcgccg ccgccattgc
     9601 agcccaaatc ctaggcttgc cccatccctt ttcacaacgc ctgcgccacg ccctacaccc
     9661 caaccgcacc atcatccgcg ccatcgtcag aaggaaggat ctaacccctt aagcctttgc
     9721 cgcatgccgt cccgaccaca gaaaatcata ttcaaataaa cataccaatc ctgacggtaa
     9781 aatatcgaaa acacccataa cgttttcttc ccaaaataag gagtaaggaa tgcctaacaa
     9841 attgcctttg ctgtccaaga tgaatgttcc gtaacattcg aagacttcct taaccctgcc
     9901 agccgtttcg gtttctgtac cccctgttag ataaggaagg aatgaaaaag ccctgttgat
     9961 tgtatgaagt aaagggcgtt aatttttgtt aatcttccct tctcaggaac tcaatatata
    10021 aggatgttaa t
//
LOCUS       DAXHTR010000076         9891 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.076, whole genome shotgun sequence.
ACCESSION   DAXHTR010000076 DAXHTR010000000
VERSION     DAXHTR010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9891
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.076"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(129..1175)
                     /gene="adhP"
                     /locus_tag="ACFNML_001292"
     CDS             complement(129..1175)
                     /gene="adhP"
                     /locus_tag="ACFNML_001292"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFC6392671.1"
                     /translation="MKMQAVVVNKNVAGDVEVIEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(1458..1847)
                     /locus_tag="ACFNML_001293"
     CDS             complement(1458..1847)
                     /locus_tag="ACFNML_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HFC6392672.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLTEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFKITLSEVSPDHFTLQAAPDPMTNE
                     GETCVVTLNDGGTIAASGTNQSCPGFD"
     gene            2073..3251
                     /locus_tag="ACFNML_001294"
     CDS             2073..3251
                     /locus_tag="ACFNML_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HFC6392673.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDAFAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            3317..5251
                     /locus_tag="ACFNML_001295"
     CDS             3317..5251
                     /locus_tag="ACFNML_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HFC6392674.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDDIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARAEKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(5664..6446)
                     /locus_tag="ACFNML_001296"
     CDS             complement(5664..6446)
                     /locus_tag="ACFNML_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFC6392675.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAVKAKSAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            6585..8774
                     /locus_tag="ACFNML_001297"
     CDS             6585..8774
                     /locus_tag="ACFNML_001297"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFC6392676.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILSIQTTFLDEPPLPESWRDLLSFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGGMTMAALKQVNAQA
                     AKLIEDWAEQGWIETTEAAKPVLRSCNGQASHSEFVLNADQQKASDAIQTALGRFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVGNRFADVPTAVLH
                     SQMAAGRRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQGGCPIILGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPYKCPDCGNQDLTAVGHGTQRVEETLRTFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNKIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFAMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            complement(8820..>9891)
                     /locus_tag="ACFNML_001298"
     CDS             complement(8820..>9891)
                     /locus_tag="ACFNML_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6392677.1"
                     /translation="RPFFVGAAVLAILGALVFFINPGAIVLHRQIFLELMLPAAYGGF
                     LTAALLDWTGFSGNLKPVATLMAALLLAASAILPFSPQTASFFVAAYWLVLLLFCARL
                     IWLDRNTDNFALLMLLAAFTVFQTAYAVSGDLNLLRAQVHLNMAAVMFVSVRVSILLG
                     AEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLPAQTAGFTSLAVGFILLAKL
                     RELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGSVM
                     MVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRTFLMNVNPIFFITIPAILTAVV
                     FTLYLFTFVPIFRANAFTGDPE"
BASE COUNT         2293 a         2563 c         2895 g         2140 t
ORIGIN      
        1 tgacgtgttg cgatacgggt tttcagacgg tatttcaaac agaacagatt aaaatcaaat
       61 ccaaatccat aaaaaatgcc gtctgaacag cgttcagacg gcatttcgat gtgtatcgcc
      121 acgtcaaatc agtggtgatg accgcagccg cattcttttt tcatatcgat caccatacgg
      181 ccggtgattt tgccttcgcg catttcttgg aaaatggcgg gtgcttcatc caaagcacgc
      241 agttggactt tcggcacaac caaaccttcc gcgccgaatt ggaaggcttc ttccaaatct
      301 ttgcgcgtgc cgaccaaaga gccgaccact tcgatgccgt ccaaaaccaa acgcgggatg
      361 gacaaatcca tcgattccgg cggcagtccg atggcaacca cacgtccgcc cgcgcggacg
      421 caattcacgg cagagttgaa tgcggcagca gatacggcgg ttacgaccgc agcgtgtgcg
      481 ccgcctgttt tttcctgaat cactttggca gcgtcctctt tggcggcgtt gacaaccaaa
      541 tccgcgccgg tttctttggc aaacgccagt ttgtcgtcgt tgatgtcgat ggcgacaacg
      601 tgcgcgccga atactttttt cgcgtattgg acccccaagt tgcccaaacc gcccgcgccg
      661 tagatggcaa tccactgtcc cggacgaaca ctggaaactt taatggcttt ataagtggtc
      721 acgccggcac aagtaatgct ggaagcttgc gcaggatcca aaccttctgg gactttgacc
      781 gcgtaatcgg cactcacgat gcagtaggtc gccataccgc cgtcggcggt gtagcccgcg
      841 ttcaatacag agcggcacag ggtttcgcgg ccggtattgc agtattcgca cgagccgcag
      901 ctttggaaca gccaggcgat gctgacgcgg tcgccgactt tcagattttt cacaccgtcg
      961 gcaacttctt taaccaaacc gatgccctcg tgtcccagca cgcggcccgg tttttcgccg
     1021 tagtcgcctg ccgcaacgtg caggtcggtg tggcacacgc cgcaatattc gacttcgacc
     1081 aatgcctcgc cgtattccaa cgggcgaacc tcgcgttcga ttacttctac atcacccgct
     1141 acatttttat tcacaacaac tgcctgcatt ttcatgattg cgctccttag ttacggcaaa
     1201 aaaacctgta aacggaatgt tgtccgatat aagggtaagc atacgcttcc gcatctcaca
     1261 ggtcaagtgg tatgttgttg aaaaatatag attatatgtt atattataac atcttggaaa
     1321 ggcacggcat cggggcggtt gccggatgag gggcggcagg tttcaagttt gaaaaaccgg
     1381 acggcaaacc cgtaaagata ccgtctgaag ctgtgtccgg acggcatctt tacgggtttg
     1441 cgggcttcgg cggaggatta gtcgaagccg gggcaggatt ggtttgtacc ggaagcggca
     1501 atggtaccgc cgtcgttgag cgtaacgacg caggtttcgc cttcgttggt catcggatcg
     1561 ggggctgcct gaagggtgaa gtggtcgggg ctgacttcgc ttaaggtaat cttgaaatat
     1621 tcgttttgtt tcagtttgtt tttgtcgtag gttttaaacg tccctttttg gcggtagtaa
     1681 cgttccatgg tctgcgcgtt gtgcagcagg gtcgtcctga cttccgtcag gcggacgcgc
     1741 cggatgtagg ttttatagga agggtaggtg atgagcgtca ggatgccgag gatggcgacg
     1801 gcaatcatca gctcgagcag cgtaaagcct ttttgaacgt ttttcatagc aatgtgtttc
     1861 catttgtttg tcggtcggcg gcattataac gccgtatcgg aaatggcgga atatgtaaac
     1921 ggattgaaat tttcgggaaa gcagattgta taagccattt aaaacaaatg gttattttta
     1981 ttgtcggcag tttgccgcct tggatggggc agggacttgc ggtagaatcc gcttccgatt
     2041 tatgggattg acgcatacag agaattgaaa acatggcaaa aatgatgaaa tgggcggctg
     2101 ttgcggcggt cgcggcggca gcggtttggg gcggatggtc ttatctgaag cccgagccgc
     2161 aggctgctta tattacggaa acggtcaggc gcggcgacat cagccggacg gtttctgcaa
     2221 caggggagat ttcgccgtcc aacctggtat cggtcggcgc gcaggcatcg gggcagatta
     2281 agaaacttta tgtcaaactc gggcaacagg ttaaaaaggg cgatttgatt gcggaaatca
     2341 attcgacctc gcagaccaat acgctcaata cggaaaaatc caagttggaa acgtatcagg
     2401 cgaagctggt gtcggcacag attgcattgg gcagtgcgga gaagaaatat aagcgtcagg
     2461 cggcgttgtg gaaggatgat gcgacttcca aagaggattt ggaaagcgcg caggatgcgt
     2521 ttgccgccgc caaagccaat gttgccgagc tgaaggctct aatcagacag agcaaaattt
     2581 ccatcaatac cgccgagtcg gaattgggct acacgcgcat taccgcaacg atggacggca
     2641 cggtggtggc gattctcgtg gaagaggggc agactgtgaa cgcggcgcag tctacgccga
     2701 cgattgtcca attggcgaat ctggatatga tgttgaacaa aatgcagatt gccgagggcg
     2761 atattaccaa ggtgaaggcg gggcaggata tttcgtttac gattttgtcc gaaccggata
     2821 cgccgattaa ggcgaagctc gacagcgtcg accccgggct gaccacgatg tcgtcgggcg
     2881 gttacaacag cagtacggat acggcttcca atgcggtcta ctattatgcc cgttcgtttg
     2941 tgccgaatcc ggacggcaaa ctcgccacgg ggatgacgac gcagaatacg gttgaaatcg
     3001 acggcgtgaa aaatgtgctg attattccgt cgctgaccgt gaaaaatcgc ggcggcaagg
     3061 cgtttgtgcg cgtgttgggt gcggacggca aggcggcgga acgcgaaatc cggaccggta
     3121 tgagagacag tatgaatacc gaagtaaaaa gcgggttgaa agagggggac aaagtggtca
     3181 tctccgaaat aaccgccgcc gagcagcagg aaagcggcga acgcgcccta ggcggcccgc
     3241 cgcgccgata aacgaatatg ccgtctgaac acggaaacgg tttcagacgg catttgttat
     3301 tgatttacgg aatattatga gcttgatcga atgtaaaaac atcaaccgct atttcggcag
     3361 cggcgagaac cgcgtccata ttttgaaaga catcagcctg tcgatagaga agggcgattt
     3421 tgtcgccatc atcgggcagt ccggttcggg caagtccacg ctgatgaaca tactcggctg
     3481 tttggatacc gccggttccg gttcgtaccg aatcgacgac atcgaaactg ccaaaatgca
     3541 gcctgacgag ctggcggcat tgcgccgcga acgcttcggt ttcatcttcc aacgctacaa
     3601 cctcttaagc tcgctgaccg caagggacaa cgtcgcgctg ccagccgtct atatgggcgc
     3661 gggcggcaaa gagcgttccg cgcgtgcgga gaagttgttg caggatttgg gtttggcaag
     3721 caaagagggc aacaagcccg gcgaactctc gggcggacag cagcagcgcg tctccatcgc
     3781 ccgcgccctg atgaacggcg gagaaatcat cttcgccgac gagccgaccg gcgcactcga
     3841 taccgccagc ggcaaaaacg tgatggaaat catccacaag ctgcacgaag ccgggcatac
     3901 tgtcattatg gtcacgcacg accccggcat cgccgccaat gccaaccgcg tcatcgaaat
     3961 ccgggacggc gaaatcattt ccgacacctc gaaaaatccc gaaatccccg caagcaatgt
     4021 cgggaggatt cgggaaaaag cctcgtggtc gttttattac gaccagtttg tcgaagcctt
     4081 cagaatgtcg gtgcaagcag tattggcgca caaaatgcgt tcgcttctga cgatgctcgg
     4141 catcatcatc ggcatcgcgt cggtggtttc cgtcgtcgca ttgggcaacg gttcgcagaa
     4201 aaaaatcctt gaagacatca gttcgatagg gacgaacacc atcagcatct tcccggggcg
     4261 cggcttcggc gacaggcgca gcggcaggat taaaaccctg accatagacg acgcaaaaat
     4321 catcgccaaa caaagctacg ttgcttccgc cacgcccatg acttcgagcg gcggcacgct
     4381 gacttaccgc aataccgacc tgaccgcttc tttgtacggc gtgggcgaac aatatttcga
     4441 cgtgcgcgga ctgaagctgg aaacggggcg gctgtttgac gaaaacgatg tgaaagaaga
     4501 cgcgcaggtc gtcgtcatcg accaaaatgt caaagacaaa ctctttgcgg actcggatcc
     4561 gttgggtaaa accattttgt tcaggaaacg ccccttgacc gtcatcggcg tgatgaaaaa
     4621 agacgaaaac gctttcggca attccgacgt gctgatgctt tggtcgccct atacgacggt
     4681 gatgcaccaa atcacaggcg agagccacac caactccatc accgtcaaaa tcaaagacaa
     4741 tgccaatacc caggttgccg aaaaagggct gaccgatctg ctcaaagcgc ggcacggcac
     4801 ggaagatttc ttcatgaaca acagcgacag catcaggcag atagtcgaaa gcaccaccgg
     4861 tacgatgaag ctgctgattt cctccatcgc cctgatttca ttggtagtcg gcggcatcgg
     4921 cgtgatgaac atcatgctgg tgtccgttac cgagcgcacc aaagaaatcg gcatacggat
     4981 ggcaatcggc gcgcggcgcg gcaatatttt gcagcagttt ttgattgagg cggtgttaat
     5041 ctgcgtcatc ggcggtttgg tcggcgtggg tttgtccgcc gccgtcagcc tcgtgttcaa
     5101 tcattttgta accgacttcc cgatggacat ttcggcggca tccgttatcg gggcggtcgc
     5161 ctgctcgacc ggaatcggca tcgcgttcgg ctttatgcct gccaataaag cagccaaact
     5221 caatccgata gacgcattgg cacaggattg aggttggaca aagatgccgc ctgaagctgc
     5281 agaaccggtc attttggagc agaaacttat tggacaaaaa cggtttctta gattctacgt
     5341 tccaaattcc cacttgcgtg ggaatgacgg cggcgggggt tcgatgattg cacacaacgc
     5401 tcgagtcccg tcattcccgt aaagacggga attcggttcg ttcggctttg cttgtttcgg
     5461 ataaatcacg gtaactcaat attccagatt cccgcccgcg tgggaacggc ggcggggctt
     5521 cgtattgttc aatttattat tttcaatcat tcaatgggtt aggatgtgtt tgttggcgtg
     5581 ctaactttca gggcggattg gttttcaggc ggcatttccc gcaaaaaagg cttggaattt
     5641 tccaagcctt ttttgcggat ggattattga tttttgcgga tgatttcctc cagttggggc
     5701 atcgggctgt agccgctttg gctgcgcccg ttggggaaga cgagggtcgg cgtgccgttg
     5761 aagccgaatt gttcgcccaa ggaagtggtt tccgcgacgg gattgtcgca gatgctgccg
     5821 ccgaccggga atttgccttt acgcatccaa tccgtccacg ctttggcgcg gtcgggctga
     5881 caccataaga tttgcgcctt gcgcgcggca tcagggtgca ggccggcaat gggcatcata
     5941 aagctgtaaa ccgtcacgtc ggtcattttt tcaaactcgt gttccaagcg tttgcagaac
     6001 ggacaatcgg ggtcggagaa gacggcgact ttcagcttgc cgttgccgcg cacttctttg
     6061 atggctttgt ccaaaggcag ggaggcgaag tcgattttgt tcaaatcggc ggcgcgttct
     6121 tcggtcaggt ttttgcgcgt gtcgatgttg atgagttcgc cgacgaacat atagccgcct
     6181 tcggcatcgg tgtagataat ctgcctgccg ctgacgacga cttcgtaaat gcctttgacc
     6241 ggtgtttcgc tgacgctcaa cactttcaaa tcttgggcgg aataggtttt ttccaaacgc
     6301 gctttcaaag aggcggcaac ggatttgccg gcggacttgg ctttgacggc gggttcggcg
     6361 ttggcattgg aaacgggcgt ttgcccgcaa gccagcagcg ggaggacggt aaagggggtc
     6421 aagattttga ttaacttggt tttcatataa agatgattgc gcgtgttgga aaagcggaat
     6481 tgtatcaaat ctctgttgcg cctgcattgc gcctaggctc aatttatcgt ctgaaaatag
     6541 cttccgcctg ttaaaatacg caaaaaaatg atttgcctgc ctgtatgatc taccaccgca
     6601 tcgccgtaaa cgtgccgctt tcagacggcc ttttgactta ttcccattcc gatccgcttc
     6661 ctccgggaac gcgggtgctt gtgcctttcc gcaataaaac cgtggtcggg atggtgtggg
     6721 aaacggatat tgcgccggat atggatacgg cgcggatttt gagtattcag acgacctttt
     6781 tagacgagcc gccgttgcct gaaagctggc gtgatttgct ctcgtttacg tcgcgttatt
     6841 accactatcc gacggggcag gcggtgtttg ccgcgctgcc gcagggtttg aaggaaacgc
     6901 gcgcggtgga aatgccgcag ccgccgttgt tttatgcgtt gaacgaagcg ggcagggcgc
     6961 aaacgccgcc gccggcgcgg ttcaacaaaa aagcggcttt gtgggacgcg ctgctgtcgg
     7021 gcgggatgac gatggcggcg ttgaagcagg tgaacgcgca ggcggcgaaa ttaatcgaag
     7081 attgggcgga gcagggctgg attgaaacga cggaagcggc gaagcccgtt ttaaggtcgt
     7141 gtaatgggca ggcttcgcac tctgaatttg tattaaatgc tgaccagcag aaggcttccg
     7201 atgccattca gacggcattg ggacgcttcc agccgtttct gctgtacggc atcaccggca
     7261 gcggcaagac cgaagtgtat ttcgatgcga tggcgaaagt gttggctcag gggcggcagg
     7321 tgttgtttct gttgcccgaa atcaacctca cgccgcagct tttgaagcgg gtgggaaatc
     7381 gttttgccga cgtgccgacc gccgtgttgc acagtcagat ggcggcaggc aggcgcacgc
     7441 aggattattt gcgcgcgatg ttggggcagg cgaaattggt catcggcacg cggctggcgg
     7501 tgtttacgcc gatggatgat gtcgggctga ttgtagtcga tgaggaacac gacggctcgt
     7561 tcaaacaaga caacgaattg cgctaccacg cccgcgattt ggcggtgtgg cgggcgaagc
     7621 agggcggctg ccccatcata ttgggcagtg ccacgcccag cttggaaagc tggcacaagg
     7681 cgcaaagcgg cgcgtaccgc ctgctgcaac tgaccgaacg cgcccatacc gccgcgcaac
     7741 tgccgcaagt ggacattctc aacgtaggcc gtctgaaact cgacaacggt ttctcgccgc
     7801 aagccttgca gcttttgaaa cagaattttg aagcgggcgg tatgtcgctg gtgtacctca
     7861 accggcgcgg cttcgcaccc gcgctgtttt gcggcgactg cggccatacc ttcggctgcc
     7921 caaactgctc cgccaaaatg gtactgcacc aacgcgcccg ccaactgcgc tgtcaccact
     7981 gcgaccaccg cgaacccatc ccgtacaaat gccccgactg cggcaaccaa gacctgaccg
     8041 ccgtcggcca cggcacgcag cgcgtcgaag aaaccctgcg caccttcctg cccaaggcag
     8101 ccgtcgtccg tgttgacagg gacagcaccg cgcacaaaaa tgactgggcg gatttgtacc
     8161 gccgcatcgc cgacaacaaa atcgacattt tggtcggcac gcagatgctt gccaaaggcc
     8221 atgatttcgc gcggctcaac ctcgttatcg tattgaacgc cgacggcagc ctgtacagcg
     8281 cggactttcg cgcgccggaa aggctgtttg ccgagctgat gcaagtgtca ggcagggcgg
     8341 ggcgcgccga caaacccggc aaggtgttga tacagaccca actgcccgaa catcctgtct
     8401 tcgccgccgt caaagcgcaa gactatgccg tgtttgccga aaacgaattg aacgagcggc
     8461 aaatgttcgc catgccgccc ttcggtttcc agaccgccgt ccgcgccgat gcgccgcgcg
     8521 ttgccgatgc gatggaattt ctcaatgccg ccaaagaaac cctcgccccg ctgttgcccg
     8581 aaagcgtttc ccagttcggc gcggccccga tgctgatggt gcgcctcgcc gaacgcgaac
     8641 gcgcgcaaat cttcctcgaa tcgacatccc gacaggattt gcaccgtgcc gtgagtttgt
     8701 gggtgcaggt gttgcagcaa aaccgcgacg gcaaaatcag atggtcggtg gatgtcgatc
     8761 cgcaggaggc ttgattatcg gcaatacgat gccgtctgaa accgtttcag acggcatttt
     8821 tattccgggt cgcccgtaaa cgcattcgcc cgaaaaatcg gaacaaacgt gaaaagataa
     8881 agcgtgaaca cgacggcggt cagaatcgcg gggatggtga tgaagaatat cgggttcacg
     8941 ttcatcaaga aagtgcgcga gacggcggcg gcgaagagga tggggacggc aatgcggcag
     9001 agtttggggt agtcgagttt ggtaaagccg ctgtgccaca gtccggcggt cagccacacc
     9061 atcatcacgc tgcccatcat gccaccgagg gtaatcaggt gcaggggcgc ggaggcgggc
     9121 aggttttgta atttcgccgc gcctgtccac aaatagcctg cggcggcaaa gagttggagc
     9181 aggtaataag tgcggacgta gtgtttgcgc aggagttcgt gatggtgaag ctcacgcagc
     9241 ttggcaagca ggataaagcc gacggcgagc gaggtaaaac cggcggtttg cgcaggcagc
     9301 caaagttcgg cggcggcgtg caggagcagg aaggtaatgg cgatgttttt atagacgaca
     9361 ttggggatga atactgggtc tttcagacgg cattctttca gggcttccgc gcccaaaaga
     9421 atactgacgc gcacggatac gaacatcacc gccgccatat ttagatgcac ttgcgcgcgc
     9481 aacaggttca aatcgccgct gacggcatat gccgtctgaa aaacagtgaa cgcggcaagt
     9541 aacattagca gggcgaagtt gtcggtgttt cggtctagcc aaatcagccg ggcgcagaac
     9601 agcagcaaca ccagccaata ggcggcgacg aaaaacgagg cagtttgcgg cgaaaagggc
     9661 agtatagcgg atgcggcgag caataatgcc gccatcaaag tcgcgacagg tttcaggtta
     9721 cccgaaaaac ccgtccagtc caacaaagcc gcagtcaaaa aaccgccgta tgccgccggc
     9781 agcataagtt ccaagaaaat ttggcggtgc aggacgatgg caccggggtt gatgaaaaac
     9841 accagcgcac cgagtatggc aagcaccgcc gcgccgacga aaaacggccg c
//
LOCUS       DAXHTR010000077         9798 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.077, whole genome shotgun sequence.
ACCESSION   DAXHTR010000077 DAXHTR010000000
VERSION     DAXHTR010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.077"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(12..1307)
                     /locus_tag="ACFNML_001299"
     CDS             complement(12..1307)
                     /locus_tag="ACFNML_001299"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFC6392678.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMGEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAVRPQHWEELQQWHEHAQRHYGASHYQL
                     WDKAELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGVAGAAAEAGAQIFEQSPMTRIEP
                     HQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFQPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     DDVKIEYSWGGECDITANLVPHFGRLAANVFYAQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1433..2320)
                     /locus_tag="ACFNML_001300"
     CDS             complement(1433..2320)
                     /locus_tag="ACFNML_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6392679.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGTLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2320..3285)
                     /locus_tag="ACFNML_001301"
     CDS             complement(2320..3285)
                     /locus_tag="ACFNML_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFC6392680.1"
                     /translation="MNLNKLKNKLFRRPGQRAVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTTIDEDLGRLNIAVSYQNYADIFQNFWSTLNPFGDGENSNIYLMTY
                     WSSIKTALTTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3301..4425)
                     /gene="potA"
                     /locus_tag="ACFNML_001302"
     CDS             complement(3301..4425)
                     /gene="potA"
                     /locus_tag="ACFNML_001302"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HFC6392681.1"
                     /translation="MTATTASSTKPYLQIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLSNG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4621..4728)
                     /locus_tag="ACFNML_001303"
                     /pseudo
     CDS             complement(4621..4728)
                     /locus_tag="ACFNML_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="ACFNML_001304"
     CDS             complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="ACFNML_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFC6392682.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5299..6234)
                     /gene="secF"
                     /locus_tag="ACFNML_001305"
     CDS             complement(5299..6234)
                     /gene="secF"
                     /locus_tag="ACFNML_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFC6392683.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGTALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6238..8091)
                     /gene="secD"
                     /locus_tag="ACFNML_001306"
     CDS             complement(6238..8091)
                     /gene="secD"
                     /locus_tag="ACFNML_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFC6392684.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALKNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="ACFNML_001307"
     CDS             complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="ACFNML_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFC6392685.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8697..>9798
                     /locus_tag="ACFNML_001308"
     CDS             8697..>9798
                     /locus_tag="ACFNML_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HFC6392686.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACAGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALVHSSKYLNRLESCLPQKGKISRL
                     DNDTAISTGSLSAARFAAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGVMHAVAEYRLNRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRAFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRY"
BASE COUNT         2495 a         2609 c         2439 g         2255 t
ORIGIN      
        1 gcctgcgacg cttaacggct gtctttccac tgataatatt tcgagccgag gaaagaaccg
       61 agtttgcgca aaaacggttg caggataata ttcggctggc gcaaccgctc aaacggcttc
      121 agacggcatt cgtcccctaa aattgcttcg gcaaccgcca gacctgcaat gcctgttatc
      181 gccatcccgt gtccggaata accttgtgca taaaaaacat ttgcggccaa acgtccgaaa
      241 tgcgggacaa ggttggcggt aatgtcgcac tccccgcccc acgaatattc gattttgaca
      301 tcgtcaagct gcggaaaaac tttaagcata tcttggcgga caagctcggt catacgcgca
      361 ggattgtcga taaactcgtt atccttaccg ccgaaaagca gtctgccgtc cgcgctgagg
      421 cggtaataat ccaaaatatg gcggttgtcg catactgcca tattgttacg gataagccct
      481 tttgcgcgcg cccccaaggg ttcggtcgca ataataaagg tgctaacggc aatcgccttg
      541 cgttccaaag gctggaatat cgggttcaaa cctgcatagg tattgacggc atacaccaga
      601 tttttgcatt cgacgctgcc ttcgggcgtg taaaccagcc aaccgttttg atgcggttcg
      661 atgcgcgtca tcggggactg ctcgaaaatc tgcgcgccgg cttcggcagc ggcaccggca
      721 acacccaacg tgtaattgag cggatgcagg tgtccggata aggggtcgaa ttgtgcccct
      781 tggtacatat cgctgtcaag ctgctgtttc aactcggctt tatcccaaag ttgataatga
      841 ctcgcaccgt aatgccgttg ggcgtgttca tgccactgct gcaactcttc ccaatgctgc
      901 ggacggacgg caaccgtggc ataaccgcgc tgccaatcgc aatcaatggc atgtttgcgg
      961 acgcgttcgt ccaccagttc gaccgcctgc aaagactgtt gccaaaacca ttgcgcctgc
     1021 tccaagccga cctgtttttc aatttccccc ataccgcagg cgtaatcgct gataacctgc
     1081 ccgccactcc gtcccgacgc gccgaaaccg atacgcgcgg cttccaacac aaccgtttca
     1141 tgtccctgct ccgccaaggg caatgcagtg cacaaaccac tcaatccgcc gccgatgata
     1201 caggtatcgg ttttcagacg gcattgaagt ttcggataaa cagtatgagg attaaccgaa
     1261 ctgaaataat aagaaggcag atattcttga aaatcagggc gaatcattgt gtttgcttta
     1321 tcaggtgtat tttcggacgg aatgatacag gctgtcgggc catatcgtcc aaacagaaaa
     1381 tcggttgaag aaaacaggct gacccagtca tgcggtcagc ctgccttatt aattaatttg
     1441 ctttctcggc agccaatttt tcttggcggt aggcttctgc cgcttctcgg tcacgcttgg
     1501 ttgcctgcct catcatccaa taattgacga tgatgaccaa tgttccgatg atgccgatta
     1561 ggatggtcgc caagacattc atctgcggat cgagacccaa cttgattttg gagaaaatca
     1621 cctgcggcaa tgtggatgaa ccggggccgg agaggaatga ggtaatcacc aaatcatcca
     1681 aagacagggt aatgccgagc agaaagcctg aagcgatggc aggggcaatc aaaggcaaag
     1741 tgatgacaaa aaagattttc agcggacgcg cgcccaaatc cattgcggct tcttcgagcg
     1801 actggtcaag ctcaaccaga cgcgaacgga taacaacggt aatgtacgcc atacacagcg
     1861 tcgtatgtcc gaggaagatg gtgaaaaagc cacgatcgaa gtagagatgt tgtaaccatt
     1921 cgctgccctg caaaaatatc tgtacctgaa taatcagcag cagcatagac agaccggtaa
     1981 tcacgtcggg catcaccata ggtgcggaaa tcataccggc gaacaaggta ctgccgcgaa
     2041 aacgtttaat ccgcgccatc gcatagcctg ccagcgtgcc caaaacgacg gcagcaagcg
     2101 aagacacaac ggcaatccgc agcgacagcc aagcggcttc caagatggtg tcgttttcca
     2161 gcaatgcgcc gtaccacttg gtcgaaaagc cgccccaaac ggttaccagc ttggattcgt
     2221 taaacgaata gatgaccaaa acaaccagcg ggatatacag aaacgccagc gacagtgcca
     2281 acatcagttt caagaaccaa gataatttgg atttctgcat tatttggctc cttcttccaa
     2341 ttcgcggttt tcataatgct gaaacagggc aatcggcacg accagcagcg cgaccatcac
     2401 gacggcgacg gcggaagcca gcggccagtt gttttgatcg aagaacgcct gccacaagac
     2461 tttaccaatc atcaggtttt ccgaaccgcc gaccagctcg ggaatgacga actcgccgac
     2521 agcagggacg aaaaccagca tggagcctgc aataatgccg gttttcgaca aaggcagggt
     2581 aatcgtcaag aacgatttga ccggccccgc gcccaaatcg gaagccgctt caagcaggcg
     2641 gttgtcgagt ttcaccagtt gcgtgtatag cggcagaatc ataaacggca gataggcgta
     2701 aaccatcacc aaattgagcg aaaaggcatt gtagaacaaa tccaaaggct cgctgataat
     2761 acccatttta atcaacaggt tgtttacaat gccgttatgc ccgagcagac ccatccacgc
     2821 atagacgcgc aacaggaacg atgtccaaaa gggcagcata atggcaagca gcaaaccatt
     2881 gcggacagaa ggattggcac gagaaatcgc ataggcggtc ggataaccga ccaacagaca
     2941 aattaccgtc gtagtcagcg cagtcttaat tgaagaccaa taagtcatca gatagatatt
     3001 gctgttttca ccgtcgccga acggattgag cgtactccaa aaattttgga agatgtctgc
     3061 ataattttgg tagctgacag caatattcag acgacccaaa tcctcatcta tcgtcgttaa
     3121 aggagtaaac ggcgggatgg cgatttcttg ttcggcaaag ctgattttca gcacgatggc
     3181 gaacggaatc agaaacagca ccaaaagcca aatatacggt acggcaatca ccgcacgctg
     3241 ccccggacgg cggaacagtt tgtttttcag tttattaagg ttcattgcat tccccttaaa
     3301 tcaacggaac agtggcgtag gctggttttc cggccagctg atatagacgg tttcgtccca
     3361 agttggcggt gtaatgttgc gcacatacca gtaaggggcg gggacttggc tcttgacgac
     3421 gcgcccgttg gagagtttga tatggtatat ggcgaagctg cccaaatagg cgatttcttt
     3481 taccgtgcct ttcgcccagt tgtagtcgcc caaatattcg ggtttttctt tatataaatc
     3541 aatatcctct ggtcgaatac taacccaaag gtcctgctcg ctcggaccac ccaaaccgtg
     3601 gtcgatgcgg acgtggtttt ccaaaccttc gcattcgata acggcatagt cggcatgatc
     3661 ttcaatcacc acaccgtcaa agatgttggt ttcgccgata aactcggcag tgaagcggct
     3721 gttgggatag tcgtacacgt cgctgggtgt gccgacttgc tgcaactgac cgtcggacat
     3781 aatggcgatg cgggtcgcca tcgtcatcgc ctcttcttgg tcgtgtgtaa ccataataca
     3841 ggttacgccg acttgttcca gcgtattgac caactcaagc tgggtttgct ggcgcagttt
     3901 tttgtccaac gcgccgaggg gctcatccag cagtagaatt ttcggacgtt ttgccagact
     3961 gcgtgccaaa gcaatgcgct gctgctgacc gccggacaat tggtgcggtt tgcgtttagc
     4021 aaatttggtc atctgaacca ggcggagcat ttcttcgacg cgcgcggcga tttcgccttt
     4081 aggcattttg tcctgtttca gaccgaaggc aatgttttgt tctacggtca tatgcggaaa
     4141 aagcgcgtaa ctttggaaca tcatattgat ggggcgatca tagggtgcaa gtttggtaat
     4201 atcctgacca tcaaggataa tttttccctg attgggactt tccatacccg ccagcatacg
     4261 cagcagcgtg gattttccgc tgccggaact gcccaaaaga gcgaaaattt cgtgttgata
     4321 aatgtccaag tcgatgttat cgacagcgta attgtcacca aactttttca ccaaaccttg
     4381 gatttgcaga taaggcttgg ttgaagacgc agtggttgcg gtcataatgg caatactcca
     4441 ataaaaagac gagtaccggc aaaacggatt ttcgaatggg tgataaaaag ctgtttgatt
     4501 gctggcggga gtcgcaacgt ctgatgccgt ctgaaactct tgtgaagcgc acgggcagca
     4561 tgaaatggaa caagattcca aagaacttta tattatatta gtttatgcgg ttttcgggca
     4621 atatagtgga ttaaatttaa accagtacag cgttgccttg ccttgccgta ctatttgtac
     4681 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactataaa tatggatttg
     4741 agcttgtcgg aaagcaacag aaaagaaaac accgcccatt tttctgggcg gtgtcggaaa
     4801 gcgtaattat ttacgcagac ccaagcgggt aatcaacgcg cgatacgtat cgggctgggt
     4861 acggcgcaag taggccagca ggcggcggcg ttggctgacc attttcaaca ggccgcgacg
     4921 gctgtggtgg tcttttgggt tggctttgaa gtggggggtc aggtcgttga tgcggaaagt
     4981 caacagagcg acttgtactt cggaagagcc ggtgtcgcct tctttgcgtt ggaaatcttt
     5041 aacgatttgt gctttttgtt ctacggtcag tgccataatg aaaactccaa aaatataaga
     5101 atccccatag ggattcagac aagtttgcca agcctgaaga caacggcaaa ctccctatgc
     5161 ccaagatagg acaacgtggc attatgacac aattcccgca tttctgcaca atattttaag
     5221 accgtggaag taaccgttaa aaatgccgtc tgaagcattg tctgcttgag acggcaatgt
     5281 tcagacggca tatgcgcttc aaaccacgat ttcttctttc ttcttcggtt ctttaccgat
     5341 attgtcgcgg ctcaaaccga acattagcag aagcgggctg gcaaccaata cggaagaata
     5401 aatgccgaac acgatgccga tggtcaacgc catagaaaag ccgtgcaagg ccgtaccgcc
     5461 gaacaccagc atggatacga ccatcgcctc ggtcgaaccg tgggtaatga tggtgcggct
     5521 catcgttgcg gtaatcgcgt tgtcgatgac ttccggcacg gcatgtccgc gcatcgccgg
     5581 cttgcggaag ttttcacgga tacggtcgaa gacgacgacg gattcgttca cagaatagcc
     5641 caatacggca aggatacccg ccaagacggt cagcgaaaat tcccattgga agaaggcaaa
     5701 gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata cggcaaaacg
     5761 ccattcgaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac ctaaagccat
     5821 caatccatta cttaccaatt cctcaccaac ttgcgggccg ataaattcga cttggcgcaa
     5881 agtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca actgtgcgga
     5941 agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac ccaatgcctg
     6001 aacctgtaca tcacctattt tcagcgtatc gaggcgttcg cgcatcttat tgacatccgc
     6061 accctgctga tattggactt ccattaccgt gccgccggta aattcgacag agaaattcag
     6121 acctctggta accaaaaaga atacggcggc aataaaggta accaacgaaa taaaggtcgt
     6181 cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt ccatagctta
     6241 ctccttgcct cctgccattt cggctttcgg cttccacacc gaaccgatgg aaatattctg
     6301 caatttgcgt ctgcgtccat accacagatt gaccaacgca cggaatacga cgacggacga
     6361 atacatcgaa gtcagaatac ccaaacagtg tacgaccgca aaaccgcgta ccgggccgga
     6421 accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttggaat cgacaatggt
     6481 cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc cggcacgcaa
     6541 ttcttcgcgg atacgttcgt taatcaagac gttggagtcg attgccatac ccaaagtcaa
     6601 cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca aaataccgat
     6661 taggaacagt atgttggcac tcaatgcaat ggtagaaaag aaacccatca gacgatagta
     6721 aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga agcctttttc
     6781 gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct gcatcggcgc
     6841 ggcaagagaa ccggcacgca acagcaaaga cgtatcattg gcttcggctg tcgtcatgct
     6901 tccggaaatt tccacgcgtc cgccggtaat ggcagtacgg ataaccggcg cggttacaac
     6961 ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat ttgcggcagt
     7021 cagttcgcca aaaatgctgc cacccgcgct gtccaagctc agactgacag caggtgcgcc
     7081 catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg cccgtcagct cgacctgttt
     7141 gctgattagc agagtttcgg gacgatctcc gccgcttgaa agcagctcat aaccgctcgg
     7201 cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca tacgcaattc
     7261 caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcctgaa cgcccggaag
     7321 ctgcacgacg atacggtcgg caccggactg ctggatgacg ggttcggcca cgcccaactc
     7381 gttcacacgg ttgtgcaggg tagtgatgtt ctgtttgacc gcatcggaac gcactttatt
     7441 gaccgcctct tccgaaagcg tcaagacgat attgctgccg tctgaattca gcgttgcttc
     7501 aggaaacagc ttgcgcaact gcggcagtgc cttttgcaca tcacctgcat cctgcaaagg
     7561 gacggtcagg ctgtttccag cctgacgcac cgtgccgctg cggatttttt cgcggcgcag
     7621 ttcgcggcgg atgtcgcccg aataacgttc aaacgttttc tgcatcgccg ctttcatatc
     7681 gacctgcatg gtgaaatgca cgccgccgcg caggtccaaa cccaaaaaca tcggattggc
     7741 tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg taatataccc
     7801 ttcgcccaaa gtgttttcga tgacgtcgcg cgctttaagc tgcgtttctg tgtctttgaa
     7861 acgcactttc agtgaattgt ccacaacaaa catcccgtcg gtctgaatac ctgcgttttt
     7921 cagcgcggca tccactttgg attgagtctg ttcgttgatg atgatggctt gtcggttggt
     7981 cgatacctgc acggcgggtg tttcgccgaa taggttgggc agcgaataca ctgcggcaac
     8041 cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca tcattgttcc
     8101 ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc agaagaggca
     8161 aaaacccttc ccagccaggc aagaccggaa ggcggcatct tgaatatgcc gccctgcgtg
     8221 tcggaacatg gtcaagcctt cggttggaat tcaaaacaaa gtgccgcatt cgggctttcc
     8281 agatgcggct tgtcggcaca aatcaatcga cttttgcggc aatcgcattg cgttctactt
     8341 cgacctcgat ttttgtaccc tgtccgatat ccacggtaaa aaactgttcg ccgactctgg
     8401 ttaccttacc cttgaaacct gccgccaaga ccactttgtc gccgactttc aaggcggcaa
     8461 gcattgcctg atgcgctttg aatttctttt gctgcggacg catgatcagg aagtagaaca
     8521 ccaccataat caacactaaa ggagcaaatt gtgcaacagc ttgattcata atttatccgt
     8581 tctttctaat atggttgaaa atcgagaggg gtatataata acataagacc gtaaacaata
     8641 tatcgggttt gccgtccgta ccgaccgtat accgcagcct gcccgtccac aaacccatgt
     8701 ccttgacccg cctaatcttg aaattctatg cactgttgcg cctttttttg ggcaaaaacg
     8761 cccgcaccgc atggatttcg catcccgcct gtgccgggca cgaacccggc gcaaaccatc
     8821 ccgattcgcc cgaccgcatc ctctgcatcg agcaggcatt gcgccgcgcc ggtatttggc
     8881 agcacctcca aaccatagag gcggaagaaa tcagcgatac gcgcctcgca cttgtccact
     8941 cgagcaaata tctgaaccgt ttggaatctt gcctgcccca aaaaggcaag atttcccgcc
     9001 tggataacga cactgcaatc agcacaggct cgctgtctgc cgcacgcttt gccgccggtt
     9061 cggcagttca ggcagtcgac atggtcatga accgtaaagc atggcatgcc ttttgcgccg
     9121 cccgcccgcc cggacaccat gcgggcagcg gcaaagccgg cggattctgc ctgctgaaca
     9181 acgttgccgc cggcgtcatg catgccgttg ccgaataccg gctgaaccgc atcgccgtca
     9241 tcgatttcga tgtacactac ggcgacggta cggcagaaat attcaaagac gatccgcgca
     9301 tcctgttttt caacctgttt gaaaccgacc ttttcccctt ccccgaaaac aacgatatgc
     9361 ccgacggcgg caatatggtg cacctgccct tgccgccggg aacgggcagc cgcgcattcc
     9421 gcgaagccgt ccgcagacag tggctgcccc ggcttgccgc attcaaaccc gaactggtgc
     9481 tgctgtcggc aggattcgac gcacaccgtc tggacgaatc gggcaggctt aacctgcacg
     9541 aggcggattt tgcctggctg acacacaaaa tcattcagac ggcatcgggc tgccctggca
     9601 aaatcgtatc cgtactcgaa ggcggctaca cccttgaacc tctggcacaa tctgctgccg
     9661 agcatatccg cgtgctggca gggctgggca aatccgatgc cgcaaccgcc tatcagaaaa
     9721 cactggaccc aacaaaaaaa cggtttgcca aaccgaaaac cggacaggtg cgccaaccta
     9781 cccaatccga ccgttacg
//
LOCUS       DAXHTR010000078         9433 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.078, whole genome shotgun sequence.
ACCESSION   DAXHTR010000078 DAXHTR010000000
VERSION     DAXHTR010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9433
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.078"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(138..1580)
                     /gene="aldA"
                     /locus_tag="ACFNML_001309"
     CDS             complement(138..1580)
                     /gene="aldA"
                     /locus_tag="ACFNML_001309"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFC6392687.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEETIAREPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRGRADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1615..1815)
                     /locus_tag="ACFNML_001310"
     CDS             complement(1615..1815)
                     /locus_tag="ACFNML_001310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392688.1"
                     /translation="MDTVGSLAIGYATGGNAGAFTDANADWNNRQLHSSEYKLAEQYA
                     DIVASRLNISKETCTAIYLGIN"
     gene            complement(1824..2213)
                     /locus_tag="ACFNML_001311"
     CDS             complement(1824..2213)
                     /locus_tag="ACFNML_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392689.1"
                     /translation="MLYKCKIKKLDNTIEEAVLIEINGYELHCFISYCPFKIFENQFY
                     EVELSYEIFNDYILDKSTSNIGFRKIDDSFKYEITAELKNNHLTLQNIQFEEDEYLVN
                     YSYLDNCLVTLTADRISVSFIREIQAA"
     gene            complement(2422..3339)
                     /locus_tag="ACFNML_001312"
     CDS             complement(2422..3339)
                     /locus_tag="ACFNML_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6392690.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYMEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLNFGINRSRFQIDADGGKVILISVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYACFTPILYREPVRIWNLSPNLRGLAASCLQAVPK
                     GHLGETLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLADALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            3512..4312
                     /locus_tag="ACFNML_001313"
     CDS             3512..4312
                     /locus_tag="ACFNML_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6392691.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILNDINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLSGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTQLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            4360..5136
                     /gene="mlaE"
                     /locus_tag="ACFNML_001314"
     CDS             4360..5136
                     /gene="mlaE"
                     /locus_tag="ACFNML_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFC6392692.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLGLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            5187..5681
                     /gene="mlaD"
                     /locus_tag="ACFNML_001315"
     CDS             5187..5681
                     /gene="mlaD"
                     /locus_tag="ACFNML_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFC6392693.1"
                     /translation="MKKNILEFWVGLFVLLGVAAVGFLAFRVAGGAAFGNSGKTYTVY
                     ADFSDIGGLKANAPIKSAGVLVGRVGSIELDPKSYQAKVRLDLDSKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            5718..6308
                     /locus_tag="ACFNML_001316"
     CDS             5718..6308
                     /locus_tag="ACFNML_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFC6392694.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVSQIRQNATQVLSILKN
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDTQKQALAKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGGK"
     gene            6359..6637
                     /locus_tag="ACFNML_001317"
     CDS             6359..6637
                     /locus_tag="ACFNML_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFC6392695.1"
                     /translation="MHTELKNGILHIGGDITVKTLTAAAFTRFRQQCRLKDTCAVDLS
                     GVGRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            6634..7461
                     /locus_tag="ACFNML_001318"
     CDS             6634..7461
                     /locus_tag="ACFNML_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HFC6392696.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            7586..7948
                     /locus_tag="ACFNML_001319"
     CDS             7586..7948
                     /locus_tag="ACFNML_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HFC6392697.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7948..8331
                     /locus_tag="ACFNML_001320"
     CDS             7948..8331
                     /locus_tag="ACFNML_001320"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFC6392698.1"
                     /translation="MKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELAG
                     LILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITLM
                     PVHAATQRTVSLPATLARALEALSE"
     gene            8328..8816
                     /locus_tag="ACFNML_001321"
     CDS             8328..8816
                     /locus_tag="ACFNML_001321"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HFC6392699.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLPDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8899..9027)
                     /locus_tag="ACFNML_001322"
     CDS             complement(8899..9027)
                     /locus_tag="ACFNML_001322"
                     /inference="COORDINATES: protein motif:HMM:NF025953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFC6392700.1"
                     /translation="MAGVTVGRGSVVGAGAVVTKDIPPYSLAAGNPAVVKKNLPEG"
     gene            complement(9033..9413)
                     /locus_tag="ACFNML_001323"
     CDS             complement(9033..9413)
                     /locus_tag="ACFNML_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HFC6392701.1"
                     /translation="MGSKFFFLLLRFAGSGLPPSHMRGIGIVGRRVRGFLARRVSPHI
                     GRGVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRS
                     KNALRATRKSVRLRWRTMSGRGTG"
BASE COUNT         2192 a         2624 c         2360 g         2257 t
ORIGIN      
        1 cgtttaaatt taatccgcta tacaaagaca aaaacatcga cctcggtcat gactgattgc
       61 cggtgaagca ataaaaatgc cgtctgaacc tgaaaacggg ttcagacggc attttctatc
      121 ggggttttaa agcggcatta aatgtcggtt tccagataaa cgacttgggt ttgcagatat
      181 tcttccaaac cgtgtttgcc gtccgcgccg ccgatgccgg attttttcca accggcgtgg
      241 aagccctgca tcgcttcaaa gttttcgcgg ttgatgtagg tttcgccgaa ttgcaggcgg
      301 cgggtaacgt agaaggcttc gtttaaatta gtcgtataaa cagaactggt cagaccaaac
      361 tcgcaatcgt tagccaaggc gatgacttgg tcgagcgtgt cgaaagcgga aacgggcaga
      421 acggggccga aggtttcttc tttcataatg tccatactgt tgtcggtgtc ggtcagcagg
      481 gtaggctcga agaaataacc gcgtccttcg gcgcgtttgc cgccgcaaac caatttcgca
      541 ccttgtttga ctgcccgttc cactttttcg gcaacggctt tgacggcgcg ctcttcaatc
      601 agcgggccca tttccagcgc gcctgcttcg gcttcggcag ggttgccgta gcgtacgcct
      661 ttcatggcgg cggtcatttt ttcaatgaat gcgtctttca gactgctgtg gacataaacg
      721 cgctcggcgc agttgcagat ttgaccagtg ttgccgacgc gcgaagccaa gatggatttc
      781 actgccaagt ccaaatccgc gtctttcaaa acgatggcgg gggctttgcc gccgagttcc
      841 agcgagactt tggtgatgtt ggcggaagcg gcttccatca cttggcggcc cgcttcgacg
      901 gagccggtca ggctgaccat atcgacttgc ggatgggcgg acaaggcatt gccgatttcc
      961 gcgccggggc cgttcaccac gttgaacacg cctgcgggca gtcccacggc atcgacgatt
     1021 tcggcgaaga tgtggcagtt gatcggggtt acactgctgg gtttgacgac gatggtgttg
     1081 cccgttacca aagcggggcc cattttgcgg gcaatcagga agaaggggaa gttccacggc
     1141 aaaatgccgg cgattacgcc cagcggacgt ttgaacaata aaatattttc acgcgggcgg
     1201 tcgctttgga tgatttcgcc ttcgtaacgg cgcgcccatt cggcttggta atcgagatag
     1261 tcggcggtaa acatgacttc cacgcgcgcc aagtctttgg ttttgccgcc ttcggcaacg
     1321 atggtgtcgg tcagctcgtc ggcgcgtccg cgtatgcctt gggcgatttt gcgcaaatac
     1381 gcgccgcgtt cgaccgcagg caggcgttcc caagccggtt gcgccgcgcg tgccgccgcc
     1441 acagctcggt caacgtctgc ttttccgcct ttgggttcgc gggcgatggt ttcttcggtg
     1501 gatgggttca atacgtcgcg ccattcgccg ttgaaatcgt tttcaaagcg tccgttgatg
     1561 tacatggcca attgtttcat ttcgggttct ccagttttgt agtcagatgt agtcttagtt
     1621 tattcccaaa taaattgccg tacaagtttc ttttgaaatg tttagacgag aagctactat
     1681 gtctgcatat tgttctgcta atttatattc actcgaatgc aactgccgat tattccaatc
     1741 tgcattcgca tcagtaaaag ccccagcatt gccgcctgtg gcataaccaa tcgctaaact
     1801 gccaacggta tccacaacag gtttcaggct gcctgaattt ctctaataaa tgaaacactt
     1861 attctatcag ccgttaatgt aaccaaacaa ttatcaagat aagagtagtt tactaaatac
     1921 tcatcttctt caaattgtat attttgtagg gttagatgat tattttttaa ttctgccgtt
     1981 atttcatact tgaaactatc atctattttt ctaaatccaa tatttgaagt tgatttatcc
     2041 agtatgtaat cattaaagat ttcataggat aattccactt cataaaattg attttcaaat
     2101 attttgaatg ggcagtatga tataaaacaa tgtagttcat aaccatttat ttcaatcaat
     2161 actgcctctt caatggtgtt atctaacttt ttgattttac atttatataa cattttatct
     2221 tgcctttaga taaattgttt tgttttttgg attgttattc aaagttctcc aaaagtcttt
     2281 tcctgatttt gtatgtatcg tggtaacact accattttca ttggttccaa caaaaataaa
     2341 tttagattga cctttggaat cagtacccaa aaactgaaca tatccagaca tgcggatttc
     2401 gggttttaaa tcaaatgcgg cttaccggtt ttcttttttg gtttccgcag gcgtgctgcc
     2461 gaaatattgc ctgaatgcct gagtaaagct ggaaacgtgg cggtagccgc acaaatacgc
     2521 ggtttcgcct atgcttttgc ccccgttttg caacaggtag agcgcgtgct gcatttgttt
     2581 gtggtgcagc cactcgcttg cggtaatgcc gaaatggtcg cgcatacggc gttgcagcgt
     2641 cctttcgctg atgttcagcg cgtctgccag ccggttgact tggtgtgcgc cgtcggcaaa
     2701 cgcggcattt agggtgcggc tgaagtcttc agacggcata gcgtctgctt ccgccgtttg
     2761 ccccgccgcc ggcccgatgc cgtctgaaac cgtgtcccac aaatccgaca gcagccgcaa
     2821 aacgtccgcc tcgcggcgca atgtttcgcc caaatgcccc tttgggacgg cttgcaggca
     2881 ggatgccgcc aaaccgcgca ggtttgggga caaattccat attctgaccg gttcgcggta
     2941 aagtatgggt gtaaaacacg catactccgg acgcagcagc cattgctcca tacctttaat
     3001 tgtcattttg accgttttgc cgcctcgata aagatagcgg ctaaacagga cttcttcccc
     3061 gactgaaatc aggatgacct tgccgccgtc cgcatcgatt tggaatcggc tccggttgat
     3121 gccgaagttc aaagagcctt ccagcaaaag cacgaacgac acataaggtt cggcgaggcg
     3181 gctgctgcaa aaatcgcacc gtgccgttac cgtgccgccg tggatggaaa tgccgttgga
     3241 caaagtatcg aaacggtagc tgccctccat atagctgccg ccgccgcttt tgaccagatt
     3301 gacgaaccgg ctgctgttca aatgttctgc tttttccata gttttctgat aaaggttatc
     3361 atttgaaaga taacattttt cgccacagca aacaatccgc cgccgacaac ggcattttca
     3421 caacacttac cgtccgtatg ccgtctgaaa attgcccgtc ttcccgataa gccttaaaat
     3481 acacgccgtc aaacctaccg tccgtccgta catgagccca tcccccttta tcgaaatgaa
     3541 agacgtcgcc ttcgcgtatg gcgaccgccc gattctgaac gacatcaatt tcagtattcc
     3601 gcaaggtaat tttgccgccg tgatgggcgg ttcgggcagc ggcaaaacca cgctgatgcg
     3661 actgattaca ggacagattc gtccgcagtc cgggcaggtt ttgattgaag gacgtgattt
     3721 gtcgggcttt tcggctgacg agttgtatga acaccgccgc cgtatgggcg tattgttcca
     3781 acacggcgcg ctgtttaccg atttgtctgt atttgacaat attgcttttc cgatgcgcga
     3841 actgacgcaa ctgccggaag cggtgattcg agatttggtt ttgttgaaac tgaacgcggt
     3901 cggtctgcgc ggtgtagaaa acctgatgcc gtccgagttg tccggcggga tgtcgcgccg
     3961 cgtcgcgctt gcccgcacga ttgcgctcga ccccgaaatt atgttgtacg acgaaccgtt
     4021 taccggtctc gatccgattt ccttgggcgt gattgcccac ttgatcagcc gcgtcaacaa
     4081 ggctttgcgt tcgaccagta ttatggtcac gcacgacatt gaaaaatctt tggaaatcgt
     4141 cgatcaggtg attttcttgg cgcacggcga aattatgttc tccggctcgc cgcaggaaat
     4201 gcgcgaactg gattcgcctt gggtgcgcca gtttgtcggc ggcttggcag acggccccgt
     4261 ggcataccgt tatccggcgc aaacgtcgtt gcagcaggat ttgctcgggt aaatgagccg
     4321 gtgccgtctg aaaccgttga actttacata tagaaattta tgaactttat ccgttccgtc
     4381 ggggcgaaaa ccctcggcct tattcaatcc ctcggcagta ttacgctgtt tctgctgaac
     4441 atcttggcga aatccggtac ggctttcgtc cgtccgcgcc tgagcgtgcg ccaagtgtat
     4501 ttcgccggcg tgctgtcggt gctgattgtt gccgtttcgg ggctgttcgt cggtatggtt
     4561 ttgggtttgc agggttatac gcagttgtcg aaattcaaat ccgccgatat tttgggctat
     4621 atggtcgcgg cttcgctatt gcgcgagctg ggtccggtat tggcagcgat tttgtttgcc
     4681 agcagcgcgg gcggtgcgat gaccagcgaa atcggtctga tgaaaacgac cgaacagctc
     4741 gaagcgatga atgtgatggc ggtaaaccct gtcgcccgcg tggttgcgcc gcgcttttgg
     4801 gcgggcgtgt tttccatgcc gcttttggct tcgattttca acgtggcggg cattttcggt
     4861 gcgtatttgg tcggtgtaag ctggctgggc ttggacagcg gcattttctg gtcgcaaatg
     4921 cagaacaaca tcacgataca ttacgatgta atcaacggtc tgattaaatc cgccgcgttc
     4981 ggcgtggcgg taacgctgat tgccgtgcat cagggcttcc attgcgtccc gacttcggaa
     5041 ggcattttgc gcgccagcac gcgcacggtg gtttcgtccg ccctgacgat tttggcggtc
     5101 gattttatat tgaccgcgtg gatgtttaca gattgacagg ccgtctgaag acgaaacgcg
     5161 gaacatcgga tattcaagga actttaatga aaaagaacat attggaattt tgggtcggac
     5221 tgtttgtcct gctcggcgtg gcagcggtcg gctttctcgc tttccgcgtg gcaggcggcg
     5281 cggccttcgg caattccggc aagacttata ccgtatatgc cgatttcagc gacatcggcg
     5341 gactgaaggc caacgcgccg atcaaatccg caggcgtatt ggtcggacgc gttggttcca
     5401 tcgagcttga cccgaaatcc tatcaggcga aagtccgtct cgatttggac agcaaatatc
     5461 agttcagtag cgacgtttcc gcacaaatcc tgacttcggg acttttgggc gagcagtaca
     5521 tcgggctgca gcagggcggc gacacggaaa accttgctgc cggcgacacc atctccgtaa
     5581 ccagttctgc aatggttctg gaaaacctta tcggcaaatt catgacgagt tttgccgaga
     5641 aaaatgccga cggcggcaat gcggaaaaag ccgccgaata aacgtatgcc atcatattca
     5701 aactaggaca cttaatcatg aaaaaatcct ccctcatcag cgcattgggc atcggtattt
     5761 tgagcatcgg catggcattt gccgcccctg ccgacgcggt aagccaaatc cgtcaaaacg
     5821 ccactcaagt attgagcatc ttaaaaaacg gcgatgccaa caccgcccgc caaaaagccg
     5881 aagcctatgc gattccctat ttcgatttcc aacgtatgac cgcattggcg gtcggcaacc
     5941 cttggcgcac cgcgtccgac acgcaaaaac aagcgttggc caaagaattt caaaccctgc
     6001 tgatccgcac ctattccggc acgatgctga aattaaaaaa cgccaacgtc aacgtcaaag
     6061 acaatcccat cgtcaataaa ggcggcaaag aaatcatcgt ccgcgccgaa gtcggcgtac
     6121 ccgggcaaaa acccgtcaac atggacttca ccacctacca aagcggcggt aaataccgta
     6181 cctacaacgt cgccatcgaa ggcgcgagcc tggttaccgt gtaccgcaac caattcggcg
     6241 aaattatcaa agcgaaaggc gtggacggac tgattgccga gttgaaagcc aaaaacggcg
     6301 gcaaataacc gacaaaccat accgtccgag ggcggtatgg gttttccgag caaacgctat
     6361 gcatacagaa ctcaaaaacg gaatattgca catcggcggc gacatcaccg tcaaaaccct
     6421 gaccgccgcc gcttttaccc gcttcagaca gcaatgtcgt ctgaaagaca cctgcgcggt
     6481 tgatttgagc ggcgtcggac gcgccgattc cgcctgcgtg tcgctgctgc tcgaagtgct
     6541 gcgcggttgc aaaggcagcg tcaggctgac cggcattccc gaatccgtgc gcgcgctgtc
     6601 cgaactgtac gaaatcaaag actggctgaa atcatgaaaa aaaccgccta tgccttcctc
     6661 ctgctgatcg ggttcgcttc cgcccctgca tttgccgaaa cccgccccgc cgacccttat
     6721 gaaggctaca accgcgccgt tttcaaattc aacgaccaag ccgaccgcta cattttcgcc
     6781 cctgccgcgc gcggctaccg caaagttgcg ccgaaacccg tccgcgccgg cgtgtccaat
     6841 ttttttaaca acctgtgcga cgtggtcagc ttcggtagca atatcttgcg cttggacatc
     6901 aaacgcgcaa gcgaagacct tgtccgcgtc ggcatcaaca ccactttcgg tttgggcggg
     6961 cttatcgaca tcgccggcgc gggcggcatt cccgacaata aaaacacctt gggcgacacg
     7021 tttgcttcgt ggggatggaa aaacagcaat tatttcgtgt tgcccgtctt agggccgtcc
     7081 accgtccgcg acgcgctcgg cacgggtatt acctccgttt attcgcccaa gaatatcgtc
     7141 ttccgcaccc ctgtcggacg ctggggcacg actgccgtat ccgccgtcag tacgcgcgaa
     7201 ggtctgctcg atttgaccga cagtctggac gaagccgcca tcgacaaata cagctacacg
     7261 cgcgacctct atatgaaagt ccgtgcgcgg cagaccggtg caacacctgc cgaaggtacg
     7321 gaagataaca tcgacatcga cgaattggtc gaaagtgccg aaaccggcgc ggcggaaact
     7381 gccgttcaag aagattccgt atccgaaaca caggcagaag cagcagggga agccgaaacg
     7441 caacctggaa cacaacctta aagccatgcc gtctgaaaat ccgtgttcgg acggcattcc
     7501 cttctgtcgg aacggacagt ccgaaaccac agattgggct ataatctctt ttttgcgcat
     7561 accgcgcgac agcaaaggaa aaaccatgta cgaagtcaac cgcagcgtat tcgtcctcat
     7621 ccctttagaa cctttttgga actggctgca aaccctgccg ggcaaccacc tcgacggact
     7681 gactcttgaa gacatccaag ccgacgccaa ttcctacctt gtccgcccgt gcgaaaccgc
     7741 cgacgaagta tgggacgaaa tcgaagcccg ttttgaagac attttcgccg ccgaacttgc
     7801 cgactggtgc gaagacgaac gcgagtggcc tgcgctcgat gccgacattt tcaacgaatg
     7861 gttcgacatc cagctttcta ccgtcatcac cgacctcgaa cacgaaccgc ttgcccgcga
     7921 agccttccaa cccatcaacc tgaactgatg aagctgaccg tccgcaacta ccatctcgac
     7981 ggctacggcc atgtcaacaa tgcgcgctac ctcgaatttt tcgaagaagc gcgctgggcg
     8041 ttttttgaag aacgcggact gctgcacgag ttggcaggcc tcatactgat tgtcgcccgc
     8101 atcgacatcc gatacagccg ccccgccgtc gaaggcgacg tattgcagtt ttcatgccgc
     8161 ctgaaaaccc ccggcacgcg ccgtattgtg ctgacccaga cgattacact gccaaacggc
     8221 aaaaccgccg cagaagccga catcaccctg atgcccgtcc acgccgccac acaacgcacc
     8281 gtcagcctgc ccgccaccct tgcccgcgca ttggaagcct tgtccgaatg aaaaaaatac
     8341 tcaccgccgc cgtcgtcgca ctgatcggca tcctccttgc catcgtcctc atccccgaca
     8401 gcaaaaccgc gcccgccttc tccctgcccg acctgcacgg aaaaaccgtt tccaacgccg
     8461 acctgcaagg caaagtaacc ctgattaatt tttggtttcc ctcctgtccg ggttgtgtga
     8521 gcgaaatgcc caaaatcatt aaaacggcaa atgactataa aaacaaaaac ttccaagtcc
     8581 ttgccgttgc acaacctatc gatccgatag aaagcgtccg ccaatatgtc aaagactacg
     8641 gtttgccgtt taccgtcatg tatgatgcgg acaaagctgt cggacaggcg ttcggcacac
     8701 aggtttatcc gacttctgtc ctcatcggca aaaaaggcga aatcctcaaa acttatgtcg
     8761 gcgaacccga tttcggcaaa ctctaccaag aaatcgatac cgcgctggca caatagcgat
     8821 gccgtctgaa gcgcaaaaaa aacagaaacg atgttcctgt tttttcagat catccgcccc
     8881 gacacgttca gacggcattc aaccttccgg cagattcttt ttcaccactg ccggattgcc
     8941 tgccgccaaa gagtagggcg gaatgtcttt tgtaaccacc gcgcctgcgc ccacgaccga
     9001 accgcgtccg acggttacgc ccgccattac aatcaccctg tgccccggcc agacatcgtc
     9061 ctccaacgta atcggacgga tttccgtgta gccctcaaag cgttttttga acggtcaaac
     9121 ttgtggttat ttgaataaaa cagacattcc ggctccatca tcacattttt gccgaccacc
     9181 agcccacggc agatttcaca gtttgccccg atgcccgagc cgtcgcccaa aaccgtatcc
     9241 ggaaacacat acgccccgcg ttcgatattg accccgcgtc cgatatgcgg agaaacccgc
     9301 cgcgccaaaa aaccgcgcac ccgtctgccg acgatgccga tgccgcgcat atgtgacggc
     9361 ggcaaccccg aaccggcaaa acgcagcagc aggaaaaaga attttgatcc catcaaacct
     9421 ccctcaaaag aac
//
LOCUS       DAXHTR010000079         9299 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.079, whole genome shotgun sequence.
ACCESSION   DAXHTR010000079 DAXHTR010000000
VERSION     DAXHTR010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9299
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.079"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(91..1371)
                     /locus_tag="ACFNML_001324"
     CDS             complement(91..1371)
                     /locus_tag="ACFNML_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HFC6392702.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADLEAYAAKLSEWKL"
     gene            complement(1593..2213)
                     /gene="tmk"
                     /locus_tag="ACFNML_001325"
     CDS             complement(1593..2213)
                     /gene="tmk"
                     /locus_tag="ACFNML_001325"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFC6392703.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQSREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="ACFNML_001326"
     CDS             complement(2273..3268)
                     /gene="mltG"
                     /locus_tag="ACFNML_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HFC6392704.1"
                     /translation="MLRKLLKWSAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLRIYQIAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLIEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            3352..3924
                     /gene="ampD"
                     /locus_tag="ACFNML_001327"
     CDS             3352..3924
                     /gene="ampD"
                     /locus_tag="ACFNML_001327"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFC6392705.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFEYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            4118..5404
                     /locus_tag="ACFNML_001328"
     CDS             4118..5404
                     /locus_tag="ACFNML_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HFC6392706.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGSVMMPKPQPAVKKTAKLQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPAPVPQTPAKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQSEMLKVGIEPGGKTALRLFS"
     gene            5552..8077
                     /gene="ligA"
                     /locus_tag="ACFNML_001329"
     CDS             5552..8077
                     /gene="ligA"
                     /locus_tag="ACFNML_001329"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFC6392707.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHICRLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALELNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGNPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARRQGGFVAEEHIQELAYF
                     QALGFSLPNGNFGCFKNIDEVLAFYEHMQQKRPELPYEIDGMVVKVNSLAQQRELGFI
                     SRAPRWAIAHKFPAEEALTVVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMQETAVAVSDGIGHRQDDLFAEA
                     PAANQTQSVPLYKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVARSIAHFFAQAEQQAMIDELLAAGVAPQAQAVSLPA
                     AQYAEPQRWIARLPGFKISENKAQALWELAGKSIEGLQTDKSLPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGINKAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            8134..>9299
                     /gene="hemW"
                     /locus_tag="ACFNML_001330"
     CDS             8134..>9299
                     /gene="hemW"
                     /locus_tag="ACFNML_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFC6392708.1"
                     /translation="MHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDFNSHSLKNG
                     LPEAAYIDALLTDLQLELPNIWGRPVETIFFGGGTPSLFQAESIDRLLSGVRSLLRLQ
                     PEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNDDMLSRLGRVHNGKEALTAI
                     DTALKLFDKVNIDLMYALPNQTVQTALDDVQTAIATGATHISAYHLTMEPNTPFGHTP
                     PKGLPQDEAALDIEDAVHGALEGAGFVHYETSAFAKPAMQCRHNLNYWQFGDYLGIGA
                     GAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEFMMNALRLT
                     DGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLNDLLQ"
BASE COUNT         2180 a         2806 c         2348 g         1965 t
ORIGIN      
        1 ttgtcctgat ttttgttaat ccactatatc aatctgacag caaaatgccg cctgaaaaca
       61 gttcagacgg cattttaaac cgcaaacggc ttacagcttc cattcgctca gcttggcagc
      121 gtaagcttcc aaatctgcaa tcggacgggt tgccacgccg ctttccatcg ctgctttggc
      181 ggcagccgta gcaacgcgcg gcagcaggcg ggaatcgaac ggagtcggaa tcaggtattc
      241 cgcgccgaat tcaaatttct taccgtaagc ggcaaccact tcttcggtta cttcttccat
      301 cgccaaatcc gccaaagcat acacgcaggc gcgtttcatt tcttcgttga tggtcgtcgc
      361 gccgacatcc aacgcaccgc ggaagatgaa cgggaagcac aatacattgt tcacttggtt
      421 cgggaagtcg gagcggccgg taccgataac cacgtccgga cgggtttctt tcgccagcgg
      481 cggcaggatt tccggattcg ggttggccat ggcgaacacg atgggttttt cgttcatcgt
      541 gttcaacatt tcgggggtca gcaggttcgc gccggagagg cccaagaaga tgtctttacc
      601 tttgacggca tcggcaagta cgcgctggcc attgtcttca acggcgtaga attttttgga
      661 ttcgtccatg cggtctttgt cttcgcgggt ttggtaaatc acgcctttgg agtcgcaaac
      721 ggtcacgttt tcgcgtttca agcccaaatc cagcaattgg ttcaagcagg caatcgcggc
      781 cgcacctgcg ccggaacaca ccaaagtcgc ttcttcgatt ttacggccgg taaaacgcag
      841 ggcgttcaat acggcggcgg cggtaatgat ggccgtgccg tgctggtcgt cgtggaatac
      901 ggggattttg cagcgtttgc gtaattcgcg ttcgatgtag aaacactcgg gtgctttgat
      961 gtcttcgagg ttgatgccgc cgaaggtcgg ctctaaagcg gcgatgatgt ccacgagttt
     1021 ttgcgggtct ttttcatcga tttcgatgtc gaacacgtcc acaccggcga attttttgaa
     1081 caatacgcct ttaccttcca tcacgggttt gcccgccagc gcgccgatgt cgcccaagcc
     1141 caaaacggcc gtaccgttgg aaatgacggc gaccaagttg cctttggcgg tgtatttgta
     1201 ggcattttgc ggatcggcat ggatttccat acaaggagcg gctacgcccg gagagtacgc
     1261 caacgccaaa tctttgtcgg tcgccaaaga tttggtcggg gtaacggaaa ttttgcccgg
     1321 aacgggtaat tcgtggaact tgagggcggc ttctttcaat gagttttcca tgtgtcaatc
     1381 ctgttgcgag aaaaagattt gaatcggact gtccgaaacg ctttcgggtc atcatgttac
     1441 gggcggttcg ttaatattgc cgacgcatcc tgccttgtaa ccgagcgtaa ttctatcatg
     1501 ccgaaagttt ggcgcatacc gccattttca cctgcggcac tgccgtttga ttttcggcgc
     1561 gggtggatgc gcttgtttca atatttgccg cattaacagc cgaaatgtcc gtccaacact
     1621 ttttctatgt tgtttctgac ttcatccaag ctgcggttac tgtcgataat ggcgtaccgt
     1681 tcgggacagg cggcggcacg gttcagataa acgctgcgca cacgcatgaa gaaatctgcc
     1741 tgttcctgct cgaaccggtc tttctcgcgc gactgtccga tacgcgccat cgacacttcc
     1801 agcggcacat ccaacagcag ggtcaaatcg gggcgcaaac cgccctgcac ccaatgttcc
     1861 aaaatttcaa tgtcttcaga cggcatcccc cgcccgccgc cctgataggc gaaggttgcg
     1921 tcggtaaaac ggtcggacac gacatggatg ccgtctgaaa gtgcaggcaa tatcacgtct
     1981 tcaatatgct gcattctggc ggcgaacatc atcagtgttt ccgcacgcaa accggctttg
     2041 gtttcagggt tgagcaggat ttcgcgcaag gcctcaccga ccggcgttcc gcccggttcg
     2101 cgcgtgaaca gcacgggcag ccccctccgt tcaaaccatg ccttgatgac ggcaaggttg
     2161 gtggatttgc cggcaccgtc tatgccgtcc aaagtgatga attgcggttt catgcttgca
     2221 gttccggtaa gggtatgccg tccgaaaaca caaacttttc agacggcatg gtttattttt
     2281 tcaaaatata tttgcgaacg gcggcgttgt gttcggtcaa atcatggctg aactggctca
     2341 agcccgtacc gtccattttg gacacgaaat acaggtattt ttcaccggac ggatgggcgg
     2401 cggcatcgag tgccgccttg ccgggcagcg cgatcggggt tggcggcaga ccgccgcgcg
     2461 tgtaggtgtt gtacggcgtg tcgcggcgca ggtcggcttt acggattttg cccttgtatg
     2521 ccgcacccat gccgtaaatc acggacgggt cggtttgcag gcgcataccg attttcaggc
     2581 ggttgacgaa gacggaagcg acatggtcgc ggtcggcttc atgccctgtt tccttttcga
     2641 tcaggctcgc cataatcagc atttcataag ggtttttata aggcagcccg tcctgcctgc
     2701 tttcccatgc ctcattcagt cggcgttgca tcgccttgta ggcgatttgg taaatccgta
     2761 aatcgctgcc gcccgcatcg atttcgtagc tgtcggggaa aaactgccct tcaggattgc
     2821 cgctgaaggc atcaggggca acttccgcca tcagtttttc attgctccag cctttggtgt
     2881 cgtgttcgat gtcgggcgtt gcgtcgatga ctttcctcat atgcgaaaaa cgcgaacctt
     2941 cgataatctg cacggtaacg gaatccggcc tgccgccgcg cattttctgc aagatatccc
     3001 aagcagacac ttccgaaggc agtctgtacg tccccgtatg cagcctgttg tgcacaccca
     3061 aaacgtaggc cgccgccgtc aaaacatgcc tgctgaacac gatgcggtct tcggcaagtt
     3121 tcctgccgac cgacgaaata ccctggtttt tggcaatttt aatcctgtat gccctgccgt
     3181 tgtctttagg gacgaaaagc agcgcggcga aaacggctgc cgatacggtc aaaaaaacgg
     3241 cagaccattt caacaatttt ctcaacatgg taggattccc agcattcgga atacgtctta
     3301 aaattcgggc gacagtataa cccaaattgc ccgccggaca ggaagaacat catggacaat
     3361 cacgccgaag cacactggca aaacggctgg cttcaaagca tacgccatac cccgtcgccc
     3421 aatttcagcc cgagggaaac gggggaaacg gtttccctga tcgtgttgca caacatttcg
     3481 ctgccgccgt tcgaatatgg tacggatgcg gtggaaaagc tgtttgccaa ccggctcgac
     3541 cccgacggac atccgttctt cagcctgata cacactttgc gcgtatccag ccatttttta
     3601 atcaaacgcg acggcgaaac ggtacagttc gtttcctgcg acaatatggc ataccatgcc
     3661 ggcgtatcct cgtttcgcgg acgggaaaaa tgcaacgcat tttccatcgg catcgaactg
     3721 gaaggctgcg atttcgaacc ctttaccgaa gcgcaatacc gttcgctcga agccttgttg
     3781 gacgcaatct gccgccgcta ccccatcacg gcggtaacgg ggcaccaaga catcgcgccc
     3841 ggccgcaaaa ccgaccccgg tcattttttc gactggcggc ggatacgggg gaaagggttt
     3901 cccgtggaca gaaatgccgt ctgaaaacca cagattttag acggcatttc ggaaaatttc
     3961 cgcttatttc gacacactgc tgtcagaccc tgcaattttc gttataattc aaacgttata
     4021 accggccgtt ccatcagact gccggaaacg caagcccgat ttaaccgcag tacccgcact
     4081 tcgcgcatcc tgcatcccga aatttaaagt gattgccatg atttacatcg tactgttcct
     4141 cgccgccgtc ctcgccgttg tcgcctacaa tatgtatcag gaaaaccaat accgcaaaaa
     4201 agtgcgcgac cagttcggac actccgacaa agatgccctg ctcaacagca aaaccagcca
     4261 tgtccgcgac ggcaaaccgt ccggcgggtc agtcatgatg ccgaaacccc aaccggcggt
     4321 caaaaaaacg gcaaaactcc aagaccctgc tatgcgcaac ctgcaagaac aggatgccgt
     4381 ctacatcgcc aagcagaaac aggcaaaagc ctccccgttc aaaaccgaaa tcgaaaccgc
     4441 cttggaagaa agcggcatta tcggcaactc cgcccacacc gtttccgaac cccaaaccgg
     4501 acattccgca ccgaaacctg ccgacgcgcc ggcaaaacct gcacccgttc cgcaaacacc
     4561 tgcaaaaccg ctgattacgc tcaaagaact gtcaaaagtc gaattaccct ggtttgacgt
     4621 gcgcttcgac ttcatctcct atatcgcgct gaccgaagcc aaagaactgc acgcactgcc
     4681 acgcctttcc aaccgctgcc gctaccagat tgtcggctgc accatggacg accatttcca
     4741 gattgccgaa cccatcccgg gcatccgcta tcaggcattt atcgtgggta ttcaggcagt
     4801 cagccgcaac ggacttgcct cgcaggaaga actctccgca ttcaaccgcc aggtggacgc
     4861 attcgcacaa agcatgggcg gtcagacgct gcacaccgac cttgccgcct ttatcgaagt
     4921 ggcttccgca ctggacgcat tctgcgcgcg cgtcgaccag accatcgcca tccatttggt
     4981 ttccccgacc agcatcagcg gcgtagaact gcgttccgcc gtaacgggcg tgggtttcgt
     5041 tttggaagac gacggcgcgt tccactatac cgacacgtcg ggctcgacca tgttctccat
     5101 ctgctcgctc aacaacgagc cgtttaccaa cgcccttttg gacaaccagt cctacaaagg
     5161 cttcagtatg ctgctcgaca tcccgcactc tccggcaggc gaaaaaacct tcgacgattt
     5221 gtttatggat ttggcggtac gcctgtccgg ccagttgaac ctgaatctgg tcaacgacaa
     5281 aatggaagaa gtttcgaccc aatggctcaa agacgtgcgc acttatgtat tggcgcgtca
     5341 gtccgagatg ctcaaagtcg gtatcgaacc gggcggcaaa accgcattgc gcctgttctc
     5401 ctaaaatcga atgcaatcaa agcggatatg tcgttacggc atatccgctt tttttcgggt
     5461 aaaattcagc tgttttcaac ctatcatttt tcagacgacc tttgcccgtt tcatcccgta
     5521 tcagacaaag gtcgtctgaa aaccttctct catgaatcca aactcaaaac ataacactaa
     5581 ctttaccaat cttcttaaac cgtctgatag tgatataaaa caatttgccg cacaacacat
     5641 ctgccgcctc accgacctcc tcaaccgcta cgcctacgaa tactacaccc tcgacgcgcc
     5701 cagcgtaccc gatgccgaat acgacaaatt gttccgcgaa ctcgaagcgt tggagttaaa
     5761 ccatcccgag ctcaaactgc ccgacagccc gacccagcgc gtcggcggcg agcctttggc
     5821 gggatttgcc gaagtacgcc acgaagtgcc gatgctgtcg ctgaccaacg ctttttcccc
     5881 gcaagatgaa aacggcgtgt tcgaccatgc cgaaatgtac gccttcgacc aacgcgtgcg
     5941 cgacggcttg gacggcggca atcccgaata cgttatcgaa cccaaattcg acggcctcgc
     6001 catcagcctg ctctaccgcg acggcgtatt ggtgcaggcg gcaacgcgcg gcgacggcac
     6061 gacgggcgaa gacgttaccc aaaacatcaa aaccgtatcc aacatcccct tgcggctgca
     6121 cggcgaaaat acgcccgaac tcatcgaagt acgcggcgaa gtactgatgc aaaaagccga
     6181 ttttgccgcc ctcaataaaa gacaagccga aaacgggcaa aaaccctttg ccaatccgcg
     6241 caacgccgcc gccggcagcc tacgccaact cgattcgcgc atcactgcgc aacgcaaact
     6301 gcactttttc ccctactccg tcgcccgccg gcaaggcggt ttcgtcgcgg aagaacacat
     6361 ccaagaactc gcctatttcc aagcactcgg cttcagtctg cccaatggca atttcggctg
     6421 tttcaaaaat atcgacgaag tattggcgtt ttacgaacac atgcagcaaa aacgccccga
     6481 actgccctac gaaatcgacg gcatggtggt taaagtcaac agcctggcgc aacagcgcga
     6541 actcggcttc atctcccgcg cgccgcgctg ggcgattgcc cacaagttcc ctgccgaaga
     6601 agccctgacc gtcgtcgaag cgattgacgt gcaaatcggc aggacgggcg cggtaacgcc
     6661 ggttgcccga ttacaacccg tattcgtcgg cggcgtaacc gttaccaacg ccaccctgca
     6721 caatcaggac gaagtttccc gaaaagacgt gcgcgtcggc gataccgtcg tcgtgcgccg
     6781 cgccggagac gtgattcccg aagtcgtgcg tgtgattttt gaacgccgcc cgatgcagga
     6841 aaccgccgtt gctgtttcag acggcatcgg gcatcggcag gacgatttgt ttgccgaagc
     6901 accggctgca aaccaaaccc aatccgttcc gctctacaag ccctaccgcc tgccgaccca
     6961 ttgccccatc tgccgcagcg aaatcgaacg cgaagaaggc gaagccgtcg cccgatgcag
     7021 cggcggtatg ctttgtcagg cacaacgcgc gcaaggctta atccacttcg cctcgcgcaa
     7081 agccatggac atcgacggct tgggtgaaaa acaaatcgaa cagcttgtcg cccaagacct
     7141 cgtccgccac ttcgccgacc tctaccgcct cgatattccg accctgcaaa aaatgaagga
     7201 aacggcggac aaaaccgttg ccgaatcgga tcaaatgccg tctgaaggca gttcggtcgg
     7261 tgcgtcgggc aaacataaaa aacaaccgac caagtgggcg gaaaacatcc tcgcaggcat
     7321 agaatccggc aaaacgcccg aactcgcgcg cttcctgttc gcgctcggca tccgccacgt
     7381 cggcgaacgc accgccaaaa cgctggcaca ggcattcggc acattggaac gcgtccgccg
     7441 cgcccccgaa cccgtcctcg cctgcctgcc cgacatcggt acggtagtgg cgcgttccat
     7501 tgcccacttc ttcgcccaag ccgaacagca ggcgatgata gacgagctgc tcgccgcagg
     7561 cgttgccccg caagcccaag ccgtcagcct gcctgccgca caatatgccg aaccgcaacg
     7621 ctggattgcc cgcctgcccg gtttcaaaat tagcgaaaac aaagcccaag ccttatggga
     7681 actcgccggc aaaagtatag agggtctgca aaccgacaaa tccctccccg ccgactggca
     7741 agcatggcgc agcgaaccac aaaacgccgc cctactcgaa aacctgaaaa ccttcttcgc
     7801 gcaaatgccg tctgaagacg aggcggcgca aggttcagac ggcatcaata aagccgtagc
     7861 gggcaaaacc ttcgtgttaa ctggcacact gcccaccctc aaacgcgacc aagcccaatc
     7921 cctaatcgaa gccgcaggcg gcaaagtttc cggcagcgtg tccaaaaaaa ccgattacgt
     7981 cgtcgccgga gaagccgcgg gcagcaagct ggaaaaagcc aatgccttgg gcgtttccgt
     8041 cctcagcgaa gccgaactgc ttaccctgct cggctgaacc catgccgtct gaaccgcctt
     8101 cagacggcat tgcccacttt tccgaaccac accatgcaca ccatttcctt tccaaaccgc
     8161 acccgactca ccgccctgcc gcccctgtcg ctttacatcc acatcccgtg gtgcatcaaa
     8221 aaatgcccgt attgcgactt caattcccac agcctgaaaa acggattgcc cgaagccgcc
     8281 tatatcgacg cgctgctgac cgatttgcag cttgaattgc ccaatatttg gggcaggccg
     8341 gtggaaacga tctttttcgg cggcggtacg cccagcctgt ttcaggcgga atcgattgac
     8401 cgtttgttaa gcggcgtgcg ttcgctgttg cgcttgcagc cggaagcgga aattaccttg
     8461 gaagccaatc cggggacatt tgaaatcgag aagtttcaag gatttaaaga cgcgggtatt
     8521 acgcggcttt ctatcggcgt acagagtttc aacgacgata tgctctcaag gctggggcgc
     8581 gtccacaacg gtaaggaagc cttaaccgct atcgataccg ccttgaaatt atttgataaa
     8641 gtcaatatcg acttgatgta tgccctgccg aaccaaaccg ttcagacggc attggacgac
     8701 gtacaaaccg ctatcgctac gggcgcgacc cacatcagcg cgtatcatct gacgatggaa
     8761 ccgaacacgc cgttcggtca tacgccgccg aaaggtttgc cgcaagacga agccgccctc
     8821 gacatcgaag acgcggtaca cggcgcgctg gaaggcgcgg gttttgtcca ctacgaaaca
     8881 tcggcttttg cgaaaccagc catgcagtgc cgccacaatt tgaactactg gcagttcggc
     8941 gattatttag gcataggcgc gggcgcgcac ggcaaaattt cctatcccga ccgcatcgag
     9001 cgcaccgtcc gccgccgcca ccccaacgac tacctcgcct taatgcaaag ccaaccgagt
     9061 gaagccgtcg aacgcaaaac cgtcgctgcc gaagatttgc cgttcgaatt catgatgaac
     9121 gccctgcgcc tgaccgacgg cgtacccgcc gcgatgttgc aggagcgcac gggcgtaccg
     9181 agtgccaaaa tcatggcgca aatcgaaacg gcaaggcaaa aaggcctgct ggaaaccgac
     9241 cctgccgtat tccgcccgac cgaaaaagga cgcttgtttt taaacgattt gctgcagtg
//
LOCUS       DAXHTR010000080         9271 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.080, whole genome shotgun sequence.
ACCESSION   DAXHTR010000080 DAXHTR010000000
VERSION     DAXHTR010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.080"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(354..2466)
                     /locus_tag="ACFNML_001331"
                     /pseudo
     CDS             complement(354..2466)
                     /locus_tag="ACFNML_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(2652..3257)
                     /locus_tag="ACFNML_001332"
     CDS             complement(2652..3257)
                     /locus_tag="ACFNML_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HFC6392709.1"
                     /translation="MTRQSLQQAAESRRSVYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAVVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(3306..3704)
                     /locus_tag="ACFNML_001333"
     CDS             complement(3306..3704)
                     /locus_tag="ACFNML_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6392710.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIFGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            3812..4723
                     /locus_tag="ACFNML_001334"
     CDS             3812..4723
                     /locus_tag="ACFNML_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFC6392711.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAAGALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGTLVRLLPGYAVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            4713..6014
                     /locus_tag="ACFNML_001335"
     CDS             4713..6014
                     /locus_tag="ACFNML_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
                     /protein_id="HFC6392712.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPFYTAASPAGLVRLAVRLNTRAV
                     IADESHTFADKLTDNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAYADFDRYRE
                     VWLDRFSKQPPAGSDLFAAYRQPFPENLSAPQPAVLSDGIFLPRNGGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKNTSLMGAYLSAGCISPRLLARKSLERRLNAWTDNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGRTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTCPDIAAAAHKTDPDGT
                     FVRRHIPELAHLSADTVHTPWRFACSVDTHGYPAHPVAGIP"
     gene            complement(6082..6252)
                     /locus_tag="ACFNML_001336"
     CDS             complement(6082..6252)
                     /locus_tag="ACFNML_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392713.1"
                     /translation="MLLYRLLGGAEIECGRCPVPPMTDWQHFLPAMGTVSAWVAVIWA
                     YLMIESEKNGRY"
     gene            6409..8559
                     /gene="dinG"
                     /locus_tag="ACFNML_001337"
     CDS             6409..8559
                     /gene="dinG"
                     /locus_tag="ACFNML_001337"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFC6392714.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNMAVWTLEKLPQLTGKIAALTDKAELANLADEAASSLLDSLHEWQFHLAE
                     EPSLSLGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDGALLDRLTGDFGIFIARIEQISAVWDLLSTVPIEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLMLRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAVIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            8595..9209
                     /locus_tag="ACFNML_001338"
     CDS             8595..9209
                     /locus_tag="ACFNML_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFC6392715.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGVASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGQATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAERRTIEQESGTDPETAAWLAAEYRLPASIGDIATAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         2167 a         2737 c         2422 g         1945 t
ORIGIN      
        1 gcatttttat gcgtcaaatt tcgttaacag actatttttg caaaggtctc aaatcagcaa
       61 ttctttttgc atcgtgtgga ttttgagaca ggcctaatga ccgtacccgc ttaataaaaa
      121 atgcaccgtc aatcaaaatg gcggttttca tattgcttcc cctatatttg tcaaagatat
      181 aaaaaagccc ttgggatcgg catacccgct atgatttaat gggtaatgcg tactgccaag
      241 ggcgctattt ctttttggat tatactgatg tagataaagc ctgtcaaaaa gttcgtgtgc
      301 tgaatttacg gattatcagg attttcagac ggcattctgt tgcacgaacg ggattagaaa
      361 ctgtaattaa cccgcgccgt ataagtgcgc ggcgcgccgg gcatggcatc cgaacgccaa
      421 tatttttgat tgagcagatt ggctgcggca aaggtaatgt taacattttt atggttccag
      481 ccaagcatgg catcaactcg ggcaaagcct ggaagcgtag tcacttcttt aatttcttga
      541 gttgtaaccg tagcgtttgc ctgtaccggt tacgccgatt tcgccgtaga ggttttcggt
      601 cggggtataa cggaaaaaca ggttgccggt aacgttgctg gtgttattca aatggatgcc
      661 cactcggtcg ggattttctt tgtcttcaac gactttcgcc tgaatcacgc ccaacgaacc
      721 gcgcagatag agttttttgg ggatgatttg cccgatggcg gacaattcca cgccacgcga
      781 acggtgtttg ccgccgactg cccaagtgta gggattattt tgtgcgtcgg ggcggtagcg
      841 gatattgaag cgttcgattt ggtaggcgga caatgtggtg ctgaggcggt cgtccagcca
      901 actgctttta acgccggttt cgtattggcg ggtgtactcg gggtcagcgt tgaacacggc
      961 agaagatgac gtatcgatgc tcaaatagcc gccgcgtccg ccataaggcg cgaaaccttt
     1021 gttatacgag gcgtaaagtg tgtgaacggg gttgatgttc cacactgcgc cgatgttggg
     1081 gctgaacgag tggccgctgt actggcggct gctgccggtg agtttgtttt cggaattaaa
     1141 ggtgtatttg tcgtaacggc cgccgaggac gaatttcaaa tcgggcgtgg cggagaagat
     1201 gttttgcaca aagatgccgt aggagtcggc tttgtggcgg ttttgggtga ggataggctg
     1261 caatctgccc gaagccggcc agcttgctcg gtcgtatgga tcgatggaag cagtaaaggc
     1321 gcggctgtaa cctaaggtcg ggttgcggtg ttcgcggctg taatccatgc ctacggtcaa
     1381 gtggttttca aaacggccga tggtgtagtc gccgtttagc gtgaaattgg acgacagggt
     1441 tttgttgtcg gtctgctgcc aggcgtagtt acgtttgatt aagttgccat tttcgctgcc
     1501 tgcatagaaa tgatcaaaat cctgcgccgc cgtgcggtgg gcgagctgcc attgggcacg
     1561 ccatttgtcg ttgaaggcgt attcaaggtc ggagcgccaa acttgcagct tgtctttgac
     1621 aaaatcgttc cggtgggcga accccatgcg gtaaggcagt ccgaagcggt cgtacacgga
     1681 cttggtcgga ctgcggtcgg gcgtgcgctc cacattgtcg taggtgtatt gccccgtcca
     1741 cttcaagccg ttgtcgagtt tgacggtaat gctgggcgaa accatgacat ttttgctgtc
     1801 tatgccgctg cggaacgaat tggcgcgccc gacttcgccg gtgagacgga tggcgacgtt
     1861 tttgttcaac acttcgttaa tgtccatatt caggctgcgg tttgcccacg aaccgtaaac
     1921 cgcaccgatg ttgcggcttt gtttgaagtt ggcgtatttg ctgaccatgt tgatgacgcc
     1981 gccgccgttg gtgcggccgt aaagcacgga agacgggcct ttcaggattt ccacgcgctc
     2041 gatgttggca gtactgcggc gcacttgtcc gctttcgcgc acgccgtcgc ggtaaatatc
     2101 ggatgcgtcg gcttgaaaac cgcgcaggaa aatgctttcg ccgcgcatat cgtaggcagc
     2161 gtcgatgccg gcattgcctt cgaggatgga actcaaatcg ttcgtaccgt aatttttgtt
     2221 tttctggata ttgagcgtat cgatggtttg cggcgtttct ttgatgagct gtccgttgcg
     2281 ggtaacggcg gcttcgtcgt agttgatgta gcctttgagt acgctggtgt cggactgtcc
     2341 gaccacggaa acggtgggca gagtggcggt gtaatgttca ccattgtcct gcgtatcggc
     2401 ggcagcaaca gggaaggaag caataatcag cgtgggtaat aaagctaaat gaaatgatat
     2461 tttcattttt atactcaatt taacaaaaca accgaattat attgcctcac ggaggaaatg
     2521 agaataattt cttttaacta tattgaacat gatatttgta aacaaaggtc tcagaatgcg
     2581 gaaaactcgc cgcctgatac tgaaaaatgc cgtctgaaca gggttcagac ggcatttttt
     2641 tgaccgcgaa attatgcgcc gaacactttc aaacgttctg caacgggttc aaaggtcttt
     2701 tcacctgccg ccccttcaat accgccgata accatttgtg cgcgcaacaa ccagttttcg
     2761 gggatattcc acgctttggc aatcgccgca tcgggcaagg gattgtaatg ttgcaggttt
     2821 gcacctacgc cgaccgcggc aagtgtcgtc caaacggcat actgcaccat agcgttcgcc
     2881 tggtccgccc aaacgggaaa gttggcggca taagcaggga actgctcctg caaacctttg
     2941 acaacatttt gatcttcata aaacaaaata gttgccgcac ccgccttaaa caggttcaat
     3001 ttttgcgcgg tcggttcaaa actgtcggca ggcacgacgg cacgcagcgc gtcttcgaca
     3061 aattgccaca ccttatcatg ctcttcgcca aacagcacga ccacgcgggc agattgggaa
     3121 ttgaacgaag aaggtgtgtg caaaacggcg tgttcgacga tttggacaac ttcatctttg
     3181 ccgacgggca ggtttttatt taacgaataa acggaacggc ggctttcggc agcctgttgc
     3241 agagattgac gggtcatttc ttttcctcaa aaacattcaa caaaatacac tttaggcagt
     3301 ccggttcagg ctttacgctt gaaaaacagc ctgtccagcg accatgctcc gccgcccgct
     3361 gccgcgatat agaggaatac gaagcagaac agcactgcgg actcgccgcc gttggcaatc
     3421 gggaacaaag catttccgga agcgtgcgcc ataaaatagg caaccgccat ctgaccggac
     3481 aaaacaaacg cggcagggcg cgcaaacagg cccaacacca gcaaaatgcc gccgaaaatt
     3541 tctaaaatac cggcaagcag caacagcccg ccgggcgaac cgctgcccat ttcaatgggg
     3601 aaggcgaaga ttttcgacgt accgtgcaac aaaaacaggt aggcggttac gatacgcaaa
     3661 acagaaagca aaaccggttg gatacggttg cagcaatcgg acataaaagc tcctttgata
     3721 tttgtcttca tttcaggcgg tagagtatag atgtttcccc ttttcaaata tatactgctt
     3781 ttattgatac aacttttcca cacaagaaaa tatggacacc ctgttcagcc tcaaggtttt
     3841 ccgccaagtc gtccaaagcg gcggcttcac ccgcgccgcc ggcgcgctcg gcatctccac
     3901 cgcaatggca agcaaacacg tcagccactt ggaaaacacc gtccaagcca aactcctgca
     3961 ccgcaacagc cgcaacctca gcctgaccga agccggggaa gaatactacc ggcaatgcag
     4021 ttacgcgctc gacacgctcg acgatgccgc gcaaaaagcc gccgggggga cggaaaaacc
     4081 gcaggggctg ctgcgcgtaa cgatgccact gtggtttgcc ggcagccaga tatgcaactg
     4141 gctggcggaa taccgcgaac gttatcccga agtgacatta gaactgattt tggacaaccg
     4201 ccacgtcgat ttgattgccg aaggcgtgga tttggcgttg cgcgtttccc aaaccctgtc
     4261 cccgtcactg attgcgcgcc cactggcgga aatcgaattt gccctgcttg cctcgcccga
     4321 cttcctgagg cgcaacggcg tgccggaaac gccggaagaa gtggcggggc tgcccgccgt
     4381 cctgccgacc tacaccaacc agcagaaact cgacctcacc cgcaaatcgg acggcaaaaa
     4441 ataccggctt gaactgaccc ccgtcatccg taccgacaac acgctgatga tgcgcgaaat
     4501 gattaaggcg ggcgcgtgca tcggttatca gccgctttgg gcggcggaac acgatttgcg
     4561 ctgcggcacg ctggtgaggc tgctgcccgg atacgccgtc ccgaccgacc ggctgaatgc
     4621 cgtttatgca gacagggcat tcttaagcgc gaaagtccgc agcttcatcg attttctgaa
     4681 cgaaaaaatc gccagcagga aaggctgccg aaatgccgtc tgaaaccgcc cgcccccttt
     4741 atgcggacac gtgcgccgca cacacgctcg tctggttccg ccaaaacctc cgcatccgcg
     4801 acaacgccgc cttatgcgcc gccgttgccg aaggtttgcc cgttatcggt atttggattg
     4861 acgatgccga aacagacaac cctcgccgcg ccgcgttcta ccgccaatcc gccgccgaac
     4921 tcgcccaagg gcttgcaggg cgcggcattc cgttctacac ggcggcatcg cctgccgggc
     4981 ttgtccggct cgccgtccgc ctcaataccc gcgccgtcat cgccgacgaa tcccatactt
     5041 ttgccgacaa actcaccgac aacgcccttt ggcatgagct ggacaaacac ggcatcgcgt
     5101 taaccttcgt caacgaccgc gccgttttcg gcaaaaccga cctgataccc gacggcggca
     5161 cggcatatgc cgatttcgac cgctaccgcg aagtatggct cgaccgcttt tccaagcagc
     5221 cccccgccgg ttcggaccta ttcgcggcat accgccaacc cttccccgaa aacctttccg
     5281 ccccgcagcc tgccgtgctt tcagacggca tcttcctgcc gcgaaacggc ggtgaaacgg
     5341 cggcttggcg gcagtggcgg cggtttctcg aacaggcgga ttcctactcc gtcttaaagg
     5401 atttcccctc gcgcaaaaac acttcgctga tgggcgcgta tttgagtgcc ggctgcatct
     5461 cgccgcgcct gctcgcgcgg aaaagcctcg aacgccgtct gaacgcgtgg acggacaaca
     5521 tcatccgccg cgattttttc cttcaacttg ccttgcagca cacggatgac gacccttcag
     5581 acggcaatcc tgaacacacc ctgcgcctga cgctttggca gcagggccgg accggcattc
     5641 cgattatcga tgccgcgatg cgctgtttgc acaaaaccgg cagcctccac cccgccctga
     5701 gacgcttgag cgcggatttt ttctgccacg ttttaaacct cccccgccgc gaaggcgaga
     5761 tatggtttgc ccggcagctg accgatttcg atgcagcaat caaccaaggc aactggcggc
     5821 ttgccgcctc acggcacacc tgccccgaca ttgccgccgc cgcacacaaa accgaccccg
     5881 acggcacctt tgtcagacgg cacattcccg agcttgccca cctgtccgcc gacaccgtcc
     5941 acacgccttg gcggtttgcc tgttcggtcg atacccacgg ctatcccgcc catcctgtcg
     6001 ccggtattcc ctaagcggca cggcaaccta aaacaaatgc cgtctgaact tccgtttcag
     6061 acggcattgc gtccgaatga ctcaatatct tccgtttttt tcactttcaa tcatcaggta
     6121 tgcccaaatc accgccaccc aagccgacac cgttcccatc gccggcaaaa aatgctgcca
     6181 atccgtcatc ggcggcacag ggcaacggcc gcattcgatt tccgcgccgc ccaacaaccg
     6241 gtacagcaac atatccgcaa tccaaagcaa caacgggtat ttcaaacgct gcaaccaaaa
     6301 aaccaaccac gtccgcacga ttcacctcac cccccattcc gacaacgata ttgtaccgct
     6361 tccgtgcaat gttaaaataa gcccacttct accgccgtac aaaacgccat gctcaccgat
     6421 ttagaaaaaa acgccatccg cgaccattac caaaacatcg gcaaaaacct gcccggtttc
     6481 cgtccgcgcg cttcgcagcg ggaaatgatt gcggcggttg ccaacgcttt ttcgcggacg
     6541 ttggcgcgcg aagaaggcgg cgagccgccc aagcgcgagg gcgagagcat cgccgtaatc
     6601 gaagggccga ccggcgtggg caaatcgctt gcctacctgc tggcgggcgg catcatggcg
     6661 cagacgcgcg gcaagcggct gattgtgagc agcgcaacgg ttgccttgca ggagcagttg
     6721 gtagaccgcg acctgccgtt tttggtcgaa aaaagcggtt tggaactgag cttcgcgctt
     6781 gccaaagggc gcggccgcta tctctgcccc tacaaactct atcaactgac gcaaagcaat
     6841 gcccagcaaa acctgctcgg ctttgaagcc cccgccgtct tgtgggacag caaacccaag
     6901 cccgaagaat tgaagctgct gcgcgacatt gccgacgaat tttctgcccg acggttcaat
     6961 ggcgaccgcg acgcctggcc ggaaaaaatc gacgacgcga tttggctcaa agtgaccaac
     7021 gaccgccacg gctgcctgaa aaccgcctgt cccaaccgtc cggaatgtcc gttttacctg
     7081 gcacgcgatg tcttggaaac cgtcgatgtc gtcgttgcca accacgatct tctgcttgcc
     7141 gacatcagta tgggcggcgg cgtgattctg cctgcgcccg aaaacagttt ctattgcata
     7201 gacgaagcgc accacctgcc caaaaaagcc ctcagccgtt ttgccgccga acattcatgg
     7261 aatatggccg tttggacgct ggaaaaactg ccgcagctga ccggcaagat tgccgcgctg
     7321 accgataaag ccgaacttgc caaccttgcc gacgaagccg cctcatcctt actcgacagc
     7381 ctgcacgaat ggcagttcca tttggcggaa gagccgtctt tgagcttggg gccgtctgaa
     7441 aacgacaggc ggaccaacag cgaaccgact tggctgtggg aagacggtaa aatccccgaa
     7501 ggcctcgaaa ccaccgtttc caatacggcc attgctgcgc gcagcctgct caaacacgtt
     7561 gtcgggctga acgatgcgct ttccgccgcg cgccgcgaaa aagaacagga cggcgcgctc
     7621 ctcgaccgcc tgaccggcga tttcggcatc ttcatcgccc gtatcgaaca aatcagcgcg
     7681 gtttgggatt tgctctctac cgttcccatt gagggcgaag agccgttggc gaaatggata
     7741 acccgccgcg ccgacgacaa aaacgactac attttcaacg ccagccccat cagcagcgcg
     7801 tcccacctcg ccaacagcct gtggcggcgt gcggcaggcg cggtgttgac ctccgccacc
     7861 ctgcaatcct tgggcaactt taacctgatg ctgcgccaaa ccgggctgca atggctgcct
     7921 gaaaccacca ccctcgccct caaaagcccc tttgactttg aaaaacaggg cgaactctac
     7981 atccccccca tatacgccag ccccaaagac cccgaagcac acaccgccgc cgtcatcgag
     8041 tggctgccca agcttatttc gcccaccgaa gccatcggca cactcgtcct gttttcctcg
     8101 cgcaaacaaa tgcaggatgt cgccctgcgc ctgcccggag actacctgcc gctcctgctc
     8161 gtacaaggcg aattgcccaa agccgtcctc ctgcaaaaac accaccgggc catagaagaa
     8221 ggcaaagcca gcatcatctt cggactcgac agctttgccg aaggactcga cctgcccggc
     8281 accgcctgcg tgcaagtcat catcgccaaa ctccccttcg ccatgcccga caaccccatc
     8341 gaaaaaaccc aaaaccgctg gatagaacag cgcggcggca accccttcat cgaaatcacc
     8401 gtccccgaag ccggcatcaa actcatccag gctgtcggcc gcctcatccg caccgaacaa
     8461 gactacggcc gcgtaaccat cctcgacaac cgcatcaaaa cacagcggta cggccaacaa
     8521 ttattagccg gcctgccacc gtttaaaagg atagggtaaa cctaccgccc ttcccgcaac
     8581 agaaaggaaa agtcatgaac ttcacccgac tgctcaacca agtcttaagc acggttcaaa
     8641 aaaaaggcaa cacattctcc gacagcccgc tcaattcatt cggcggaggc gcgctggttg
     8701 ccggtgtcgc ctccatgcta ctgaacggta aaaaccgcaa aaccatcacc aaaatcggtt
     8761 cgaccgccgc tttgggctac ctcgcctacc ggggctatca gatgtggcag caaaacaaag
     8821 ggcaggcaac cgtaacacaa agcgatttcc aacctgccgg agaaactgaa gaaacataca
     8881 gccgcaccgt attgcgcacc atgatagccg ccgccgcttc agacggcatg atagacgaag
     8941 ccgaacgccg gactatcgaa caggaaagcg gcacagaccc cgaaactgcc gcatggctcg
     9001 ccgccgaata ccgcctgccc gcaagcatcg gggacatcgc caccgccgtc ggcaacgatg
     9061 aggcgttggc ggcggaggcc tatctggcgg caaggttggt ctgtgccgat ttgtcgcgga
     9121 aagaaaccgt cttcctcgcc cgcctgtcgc aggctttgaa actggatgac aatctggtgg
     9181 agagtttgga aaggcaattg gggttttgat gacacagctg gggagggaaa atcatcaaaa
     9241 aacggttatt ccgaccccgg cctcaaaacg g
//
LOCUS       DAXHTR010000081         9142 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.081, whole genome shotgun sequence.
ACCESSION   DAXHTR010000081 DAXHTR010000000
VERSION     DAXHTR010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9142
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.081"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..1938
                     /locus_tag="ACFNML_001339"
     CDS             <1..1938
                     /locus_tag="ACFNML_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HFC6392716.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDAAEIIHANSLRTPWQAADYIFGNPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRAAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
     gene            complement(2773..3456)
                     /locus_tag="ACFNML_001340"
     CDS             complement(2773..3456)
                     /locus_tag="ACFNML_001340"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFC6392717.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGRLQGLDKKQTAE
                     QYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVIPDGENLQVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVIEKFYL"
     gene            3752..6055
                     /gene="parC"
                     /locus_tag="ACFNML_001341"
     CDS             3752..6055
                     /gene="parC"
                     /locus_tag="ACFNML_001341"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFC6392718.1"
                     /translation="MNMQPHAPHTDSNTLMLGRYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEINQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPSHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKTDLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLSDENEKRKLIVKEMQADMKQFGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTI
                     LPVVILDSSGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLGDMVGRVKAGKVVMTADSGETVLPPVAVYASSFINPDCKIIAATNQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            6075..7651
                     /locus_tag="ACFNML_001342"
                     /pseudo
     CDS             6075..7651
                     /locus_tag="ACFNML_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
     gene            7644..8111
                     /locus_tag="ACFNML_001343"
     CDS             7644..8111
                     /locus_tag="ACFNML_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6392719.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDGSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            8215..8967
                     /locus_tag="ACFNML_001344"
     CDS             8215..8967
                     /locus_tag="ACFNML_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFC6392720.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAARITLHFGNAAVQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
BASE COUNT         2271 a         2857 c         2203 g         1811 t
ORIGIN      
        1 atcagtcccg aaatcttcgg cacgctcttc caatcagtca tggacgcaca agagcggcgc
       61 gaagcgggcg cacactacac cgaaaccgcc aacatcgaca aagtcatcaa cggccttttt
      121 ttagaaaacc tgcgcgccga atttgaagcc gtcaaagccc tcaaacgcga caaagacaaa
      181 aaactcgccg ccttctacca aaaaatccaa aacctgcaat tcctcgaccc ggcctgcggc
      241 tgcggcaact tccttatcgt cgcctacgac cgtatccgcg ccctcgaaga cgacatcatc
      301 gccgaagcgc tcaaagacaa aacaggcggc ttattcgaca gcccgtccgt ccaatgccgt
      361 ctgaaacagt ttcacggcat cgaaatagac gaatttgccg tcctcatcgc ccgcaccgcc
      421 atgtggctca aaaaccacca atgcaacatc cgcacgcaaa tccgcttcga cggcgaagtc
      481 gcctgccata cgctgccgct cgaagacgcc gccgaaatca tccacgccaa cagcctccgc
      541 acaccttggc aggcggcgga ctacatcttc ggcaatccgc cctttatcgg ctcgacctac
      601 caaaccaaag agcagaaaaa cgacctcgaa agcatctgcg gccatatcaa aggctacggc
      661 ctgttggatt acgtctgcaa ctggtatgtc aaagccgccg gtatcatggc gcagcatccc
      721 caagttcaga cggcatttgt ttccaccaat tccatctgcc aaggccagca ggtcgaaatc
      781 ctttggggca gccttttgaa gcaagacatc gaaatccact ttgcccaccg cacctttcaa
      841 tggacgagcc aagccgcagg caaagccgcc gtccactgca tcatcgtcgg cttccgccaa
      901 aagccgcaaa tgccgtctga aaaaaccctc tacgactatc ccgacatcaa aggcgaaccc
      961 gtaaaacacg ccgcggccaa catcaatccg tatctgattg acgcgcccga tgtgattatt
     1021 gccaagcgca gccgtcccgt acattgcgaa cctgacatgg tcaacggaag caaaccgacc
     1081 gaaggcggca accttatcct ttcaaccgat gaaaaagaag ccctgattgc cgccgaaccc
     1141 ttggcggaac aatacatccg tccctttgtc ggcgcagagg aatttatcaa cggcaaaacc
     1201 cgttggtgcc tgtggtttca cggcgtatcc gacgttaaac gcaaccacga cctgaaacag
     1261 atgccccaag ttcaagcccg tattcaggca gtcaaaacca tgcgcgaagc cagcagcgac
     1321 aaacaaactc aaaaagatgc agcaaccccg tggctttttc aaaaaatccg ccagccttca
     1381 gacggcaatt ttctgattat cccacgcgtc tcatccgaaa gccgccgttt cgtccccatc
     1441 ggttatctat catatgaaac agttgtcagc gatttggtat ttacccttcc aaacgccagc
     1501 ctctaccact tcggcatcct cagctccacc atgcacaacg cctttatgcg taccgttgcc
     1561 ggccgtctgg aaagtcgtta ccgctattcc aataccgttg tgtacaacaa cttccccttc
     1621 cccgaaagct gccggatgcc gtctgaaaac gaccgccaag acccgctccg cgccgccgtc
     1681 gaaaccgccg cccaagccgt cctcgacgcg cgcggacaat accgccgaga agcgcgggaa
     1741 gccggtttgc ccgagccgac cctcgccgaa ctctatgcgc ccgacgcagg ctataccgcc
     1801 ctagacaaag cccacgccgc cctcgacaag gcagtcgata aagcctacgg ctacaaaaca
     1861 ggcaaaaaca ccgacgacga agccgaacgc accgccttcc tgttcgagct gtaccgcaag
     1921 gcggcggcaa ttgcgtagta gttgcgtagt agttgcgtag tagttgcgta gtagttgcgt
     1981 agtagttgcg taggatagtt gcgtagggtg ggcttcagcc caccaactcc caccaactcc
     2041 caccatcttc accctaaaac cccgtcatct ccgcaaaagc aattgcgtag ggtgggcttc
     2101 agcccaccgt ttttcttcat tttcggttgt tgattggcag gctgaagccc accctgcggt
     2161 tacaaaaatc ccgtcattcc cgcgcaggcg ggaatccaga cctttcgggg cagaaactta
     2221 tcggataaaa cgatttcccc tgcatcctgg attcccactt tcatgggaat gacgggatat
     2281 aggtttccct acggacgtgt tcagattccc gccttcgcgg gaatgacggc ggagaagttt
     2341 ctgtctttcc gataacttac cgtaacttaa aatcccgtca ttaccgcaaa agcagttgct
     2401 acggttacaa aaatcccgtc atccccacga aagcgaaaat cctgcctgtc ggatttcggt
     2461 tttttcgggc gtttcgggaa cttataaatc gtcattcccg cgcaggcggg aatccagaac
     2521 gcgggacggc ggcaatattc aaaggttgtc tgaaaattca gaggttctag attcccgcct
     2581 gcgcgggaat gacggcggag cggtttctgc ttttccgata aatgaccgca acctaaaacc
     2641 catccttccc acgaaaacag aaaaacaaaa acctaaaatc ccgtcgttct cgcgaaagca
     2701 gttgctacgt ttaccgtcaa aaatgccgtc tgaacgctca agccttcaga cggcattttt
     2761 tatttcacac ccttacaggt agaatttttc gatgactttc aaattgtcgt ccaatttgta
     2821 caccaacggc tgaccggtcg ggatttccaa gcccatgatg tcttcgtcgg aaatgccctc
     2881 gatgtgtttt gccagcgcgc gcagggagtt gccgtgcgcc gccaccaaga cgcgtttgcc
     2941 gctcaaaatc gcgggggcga tttggtcttc ccaaaacggc aatacgcgct ccagcgttac
     3001 ttgcaggttt tcgccgtcgg ggataacatc ggcaggcaga tgggcatagc ggcggtcttt
     3061 gtgtgcggaa aactcatcgt ctttgtccaa aagcggcggc agggtgtcgt agctgcgccg
     3121 ccagatgcgg acttgctcgt cgccgtattg ttcggcggtt tgttttttgt ccaggccttg
     3181 cagtcggccg tagtggcgtt cgttcagccg ccatgttttg atttgcggta cgaacagttg
     3241 gtcggattct tccaaaacga tgttgcaggt cttaatcgcg cgggtcagga cggatgtgaa
     3301 ggcgatgtcg aactcatagc cgttttcttt cagtttttta ccggcggcag cagcctcggc
     3361 aagcccctgc tcgctcagct tcacgtcgcg ccagcctgta aacaggtttt tcgcgttcca
     3421 ttcgctttgt ccgtggcgga taaataccaa ttccatatcg tctccaatgt gtgaaagtgg
     3481 gaaagcctta tttataacat attttcacat tcccgtattt gattcagatt cagacacgcg
     3541 cccactatgg tttgccgttt tgatttacaa taatgccctt tgctttacat tccgcataca
     3601 caatgtttca acacacagga cgacacataa agcaccgccc tatgtgttgc cctgatttgg
     3661 aaggggttgc cccctcccaa ataaagtctg attctaccgc cccgaaggac agatgtccaa
     3721 gtggcggggg ttcaaccgaa aaggaaatac gataaatatg caaccgcacg ctccacatac
     3781 cgattccaat acgctgatgc tcggccgata cgccgaacgc gcctatctcg aatacgccat
     3841 gagcgtggtc aaaggccgcg cgctgcctga agtttcagac ggccaaaagc ccgttcagcg
     3901 gcgcattttg tttgccatgc gcgatatggg tttgacggcg ggggcgaagc cggtgaaatc
     3961 ggcgcgcgtg gtcggcgaga ttttgggtaa ataccacccg cacggcgaca gttccgccta
     4021 tgaggcgatg gtgcggatgg cgcaggattt taccttgcgc tatcccttaa tcgacggcat
     4081 cggcaacttc ggttcgcgcg acggcgacgg tgcggcggcg atgcgttaca ccgaagctcg
     4141 gctgacgccg attgcggaat tgctgttgtc cgaaatcaat cagggaacgg tggatttcgt
     4201 gccgaactac gacggcgcgt ttgacgagcc gctgcacctg cccgcccgcc tgcctatggt
     4261 gttgctcaac ggcgcgtcag gcatcgcggt gggcatggcg accgagattc cgtcgcacaa
     4321 tttgaacgaa gtgacgcagg cagcgattgc gctgttgaag aagccgacgc tggaaaccgc
     4381 cgacctgatg caatatattc ctgcgcccga ttttgccggc ggcggtcaaa tcatcacgcc
     4441 ggcggacgaa ttgcgccgga tttacgaaac cggcaaaggc agcgtgcgcg tgcgtgcgcg
     4501 ttatgaaatc gagaagctgg cgcgcggaca gtggcgcgtc atcgtaaccg agctgccgcc
     4561 gaacgccaat tccgccaaaa tccttgccga aatcgaagaa caaaccaacc cgaaaccgaa
     4621 agcgggtaag aaacagctca accaagacca gctcaacacc aaaaagctga tgctggattt
     4681 aatcgaccgc gtgcgcgacg agtccgacgg cgaacatccc gtgcgactgg tgttcgaacc
     4741 gaaatccagc cgcatcgata ccgatacctt catcaacacg ctgatggcgc aaacttcgct
     4801 ggaaggcaat gtgtccatga acttggtgat gatggggctg gacaaccgcc ccgcgcagaa
     4861 aaacctgaaa acgattttgc aggaatggct ggatttccgc accgtaaccg taacacgccg
     4921 tctgaaattc cgtttgaacc aagtggaaaa acggctgcac atcctcgaag gccgtctgaa
     4981 agtctttctg cacatcgacg aagtgattaa agtcatccgc gaatcagatg acccgaaaac
     5041 cgatttgatg gcggcgttcg ggctgaccga aatccaagcc gaagacattt tggaaatccg
     5101 cctgcgtcag ttggcgcgtt tggaaggttt caaactcgaa aaagaattga acgaattgcg
     5161 cgaagaacaa ggccgtctga acatcctttt gagcgacgaa aacgaaaaac gcaagctgat
     5221 tgtcaaagag atgcaggcgg acatgaaaca gttcggcgac gcgcgccgca cgctggtgga
     5281 agaagccgga cgcgccgtgc tgacacaaac caccgccgac gaacccatca cactgattct
     5341 gtcggaaaaa ggctggatac gcagccgcgc cggacacaat ctcgatttga gccaaaccgc
     5401 gttcaaagaa ggcgaccgcc tcaaacaaac cctcgaaggc agaacgattt tacccgtcgt
     5461 cattctcgat tcatcgggca gaacctacac ccttgatgcc gccgaaatcc ccggcgggcg
     5521 cggcgacggc gtgccggttt catccttgat cgagctgcaa aacggcgcga aacccgttgc
     5581 gatgttgaca ggattgccgg aacaacatta tttattatca agcagcggcg gctacggctt
     5641 catcgccaaa ctgggcgata tggtcgggcg cgtgaaagcg ggcaaagtgg tgatgaccgc
     5701 agacagcggc gaaaccgttt tgccgccggt tgccgtctat gcctcctcgt tcatcaaccc
     5761 cgactgcaaa atcattgccg ccaccaatca aaaccgcgcc ctcgccttcc ccatcggcga
     5821 attgaaaatt atggcgaaag gcaaagggct gcaaatcatc ggattaaacg ccggcgaatc
     5881 gatgacgcat accgccgttt cttccgagcc ggaaatcctg attgaaagcg aaggcaggcg
     5941 cggcgcagcg cacaaagacc gcatccccat ctccctgctt gaggcaaaac gcggcaaaaa
     6001 aggcaaacta ttacccatat cgggcagcct gaaacagctt tcttccccga aataaacccg
     6061 actctgcaca tactatggta atttccaata cccgcgaact tgagaaactc aaagaccgga
     6121 ttcccaatct gatcaacatc gcccgcatcg ccatcgtctt ccccctgctg attatgcata
     6181 tcttcgggtt ggaggtcagc ggacatgaaa acctgcacgc ttcgtggacg gcgtgggcat
     6241 tttatgtttg gctcgccatt tcctgctggc tgattttctt ttccatcatc catccgcatt
     6301 ggcaatggca gtcgctgaaa atgccgcgtt tcagcgcggt ggcggacatc acgatgatcg
     6361 gcgtgctgac ctacctgttc ggcggcatcg attccggctt cggcatcctg attctgccct
     6421 tcgtcgtctg ctcctgcctg ctcagctacg ggcgttaccc cctgctctat gccagctacg
     6481 ccgccatcct gctgatattc aacgccattg ccgacggcga tatcggcaaa tatccgcaca
     6541 tatcggatgc ccgaaccgcc tcggcaacct tcatcctcgt cgccgcctcc tatctttccg
     6601 ccatcttcac ctcactgtcg gtcagataca tcgacagcgc cggcaaactt gcccatgaaa
     6661 accacgccgc ctaccgccgc atcaggggct tgaaccaaat cgtactcaac cgcgttcagg
     6721 aagcggtcgt cgtcatcaac gtcgagcatc agaccatact gttcaataaa aaggcaaaag
     6781 atctgctccc catgcttgaa atcggacagc ataccgccct attcgaccct attaccgttt
     6841 tatgggacaa ggcctcttcg cgtactttcg aacgcaatat cgacacgccc gccctgaccg
     6901 cccgcatccg cgccgtgccg atgaacaaag agcaaaacaa actgctcatc ctctacatcc
     6961 gcccgcaaag cgaaattcag gcagaagccc tgtccgtcaa acttgccgca ctcggacaac
     7021 tgaccgccaa cctcacccac gaaatccgca acccgatgtc cgccatccgc cacgccaacg
     7081 gcctgctgcg cgaaaatatg gaagcggggg cggcagatcc gttcaacgcc aaattgtgca
     7141 aaatcatcga cggcaacgtc tgccgtatca acaaaatgct cgaagatatt tcctcgctca
     7201 acaagcgcaa caaaaccgaa cgcgaagcca tcggcctgat accgttttgg gaagaattca
     7261 aacaagagtt cctgctcggc catcccgatg ccgccggctg catccgccta gatatgcagg
     7321 gcaatcacct gaccgcctat ttcgatcccg cccacctgcg gcaaattatg tggaacctcg
     7381 ccaacaacgc gtggcggcac agccgcaaac agcccggctc gatttccgtc accatccgcc
     7441 ccgcgcaaaa aaacaccgtc tgtatcctct ttgccgaccg cccgaagtgc aggaacacct
     7501 gttcgaaccc ttttacacca cggcggaaaa cggcaccggc ctcgggctgt atgtcgcccg
     7561 cgaactggcg cacgccaatt tcggcgattt gacctaccta ccggaagcca aatgtttcga
     7621 actcacatta ccggaaaaaa ccaatgactg aactgcaaca ccccgtcctc gtcgtcgatg
     7681 acgaaaccga cattctcgac ctgatggaaa tgaccctgat gaaaatgggc ttgcgcgtcc
     7741 ataccgcgtc aggcgttgcc gaagccaaaa acaagctcga cagccaacgc tattcgctcg
     7801 tcctgaccga tatgcgtatg ccggacggct cggggctgga agtcgtccaa cacatcaaca
     7861 gccgcctgct cgatacgccg gttgccgtca tcaccgcctt cggcaacgcc gatcaggcac
     7921 aggaagcgtt gcgttgcggc gcgttcgacc ccgataccat gcagatacag gactatctcg
     7981 accaaatcga acgcgacatt atcgaacaaa ccctcaaaca aaccgaaggc aaccgcacgc
     8041 aggccgccaa acgcttgggc atcagcttcc gttccatgcg ctaccgtatg gaacgcctca
     8101 acatcggctg acgacaaaac ggcatccgca ccatctccgc ccacccgaaa aaatgccgtc
     8161 tgaaacggca cgggaaagcg ggttcgcccc acgcccgaac ggacacaaaa caccatgacc
     8221 gacatcctta ttgacaacac cgccaccgaa accgtccgca ccctgatacg ggcattcccc
     8281 cttgtgcccg tttcccaacc gcccgaacaa ggcagttacc tccttgccga acacgatacc
     8341 gtcagcctca ggcttgtcgg ggaaaaaagc agcgtcatcg tcgattttgc ctccggcgcg
     8401 gcacaatacc ggcgcacaaa aggcgggggc gaactcatcg ccaaagccgt caaccacacc
     8461 gcgcacccca ccgtttggga cgcaaccgca ggattggggc gcgacagctt cgtcctcgcc
     8521 tcgctcgggc tggccgttac cgccttcgag caacatcccg ccgtcgcctg cctgctttca
     8581 gacggcatcc gccgcgccct cctcaatccc gaaacgcaag acaccgccgc gcgcatcacc
     8641 ctgcatttcg gcaacgccgc agtacaaatg cccgcacttg ttcaaacaca aggcaaaccc
     8701 gacatcgtct atctcgaccc catgtatccc gaacgccgca aaagtgccgc cgtcaaaaaa
     8761 gaaatgacct acttccaccg gctcgtcggt gaagcgcaag atgaggccgc cctcctccat
     8821 accgcacgcc aaacggcaaa aaaacgcgtc gtcgtcaaac gcccccgcct cggcgaacac
     8881 cttgccggac aagcccctgc ctaccaatac accggcaaaa gcacccgctt cgacgtttac
     8941 ctgccctacg ggacggacaa gggataacgc ccataaaaca agacaccgaa aaatttgccg
     9001 ttcttatgca aacgagaaac cggtttttgc gtttcgactg ttttggataa atcatcacac
     9061 cttaaagttt gtcattccca cggaagtggg aatccagaaa tgaaaagcaa caggaattta
     9121 tcgtaaatga ccgaaaccga ac
//
LOCUS       DAXHTR010000082         9103 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.082, whole genome shotgun sequence.
ACCESSION   DAXHTR010000082 DAXHTR010000000
VERSION     DAXHTR010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9103
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.082"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(60..1316)
                     /locus_tag="ACFNML_001345"
     CDS             complement(60..1316)
                     /locus_tag="ACFNML_001345"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HFC6392721.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLFDEEETAFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWREEEILAFGRSINQPAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAVGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDSRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSILPEENELQVERFLSEHPEFEPVNCAELLAGLKIDL
                     DTGKYLRLNSARHQTDGFFAAVLQRK"
     gene            complement(1664..2005)
                     /locus_tag="ACFNML_001346"
     CDS             complement(1664..2005)
                     /locus_tag="ACFNML_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392722.1"
                     /translation="MKNNVKNWTTKEVKQSLDKFNNILIKNTFLLQYLKKEFSASSAY
                     CLSMLPEEEDIYEILVNGNIIIDLEFNKHTNETVVINVTDVDEYLKTLTNESGRVFFT
                     LAKEIGKQKNI"
     gene            complement(2208..3065)
                     /locus_tag="ACFNML_001347"
     CDS             complement(2208..3065)
                     /locus_tag="ACFNML_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HFC6392723.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADPAPLTEHLTRRAPFGHRLGIYAEELLAFWFTNAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPQGFHAFEPPLNPYGWRDVYIQD
                     WAEYGFERQEVRYHLLDRMAYLAPARAAETETLNETEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(3126..3941)
                     /locus_tag="ACFNML_001348"
     CDS             complement(3126..3941)
                     /locus_tag="ACFNML_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HFC6392724.1"
                     /translation="MTTLKPALPDYLGNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            4005..4175
                     /locus_tag="ACFNML_001349"
     CDS             4005..4175
                     /locus_tag="ACFNML_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392725.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCTSEPAVCHP"
     gene            4185..4970
                     /locus_tag="ACFNML_001350"
     CDS             4185..4970
                     /locus_tag="ACFNML_001350"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6392726.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLAKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDGWLESGDVVAANPKVLAQMLKIISAHV"
     gene            5240..7335
                     /locus_tag="ACFNML_001351"
                     /pseudo
     CDS             5240..7335
                     /locus_tag="ACFNML_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            7459..9009
                     /locus_tag="ACFNML_001352"
     CDS             7459..9009
                     /locus_tag="ACFNML_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFC6392727.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQETGFL
                     TVESHQYERGWFTSMETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAHIETEFKYAPETEKVLERFFGKQAPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGSFINSEGQFRFDTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLITAVKGEASGLFTHNPVLNIKTFRFTLPSGKIDVGGKIMFK
                     DMKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEARASIAD
                     INETLRLMVDSTVQSMAREKYLTLDGNQIDTVISLKNNALKLNGKTLQNEPDPDFDEG
                     GMVSEPQQ"
BASE COUNT         2282 a         2159 c         2410 g         2252 t
ORIGIN      
        1 gatgaaatgc cgtctgaacg ctttgaaaag ggttcagacg gcattttgtt taaacagggt
       61 tatttgcgtt gcaatacggc ggcgaagaag ccgtcggttt ggtgtcgggc ggagttgagg
      121 cgcaggtatt tgccggtatc caaatcgatt ttcaaaccgg caagcagttc ggcgcagttg
      181 acgggttcaa attcgggatg ttcggacagg aaacgttcga cttgcagctc gttttcttcg
      241 ggcaggatgc tgcaagtggc gtaaaccaag cggccttgcg gttttaccag tttggaggcg
      301 gcatcgagga tgctgtgttg ctgttccaaa aggttggcca cggtttcggc ggattggcgg
      361 tatttgaggt cgggattgcg gcgtaaagta cccaaaccgg agcagggcgc gtccaccaac
      421 acacggtcgg ctttgcctgc cagtcgggcg atacggctgt cgtgttcgct gctgatgcgt
      481 tcggggtgga tattggtcag tccggcgcgg gtcatacgcg gtttgaggtt ggcaaggcgt
      541 ttttcggcga tgtcgaaggc gtagattctg cctttattgg ccatttgcgc accgacagcc
      601 aaggttttgc cgccggcacc ggcacagaaa tcgacaatga tttcgccgcg ttttgcgccc
      661 accaataaag caagcagctg gctgccttcg tcttggactt ccagtgtgcc gtctaaaaac
      721 agttcgtgtt tgttaagcgc gattttgttt ttcaggcgga tgccccaagg cgaataaggc
      781 gttgcctctg catcggcact ttcggcttgc aacagcggca gcactttatc gcgtttgcct
      841 ttcaaagtgt tgacgcggat gtcgagcggg gcaggctggt tgatgctgcg gccgaaggcg
      901 aggatttctt cttcacgcca atgctgttgc agttgttcga ccagccattg cggcaattct
      961 gcggcggtat tcaggccgtc tgaaaactcg gttttacggg ctttcaaatt gccgaggaac
     1021 gctgtttctt cttcatcaaa caggtctttg atttggctga tgttggtgct tctgccgaga
     1081 accagtgcgg cgagtgcggc tttgcgcggc tgtgcgtgcg gacggcgcag ggcagtgctg
     1141 attttttgat agtggcgcag cgcggcaaag gcggtttcgg cgatttcgtg gcggtcttgg
     1201 cggccgagct ttttatgttc gcggaaatag gcggagagga cggcatcggc aggctgtttg
     1261 aaagtcagca tttcagccaa aactttggcg gtatggtcga gttgtgcggc gttcataatg
     1321 taatgggttc tcttactgtg ggttattcat aatcaatcta acgtccttaa aataacaata
     1381 gcatctttat cattacttag tattctatcc gcaataagaa agatgtgtta aaagtgcgca
     1441 atcgggattt gtctaaattt ctccgttaac tacaaaggca gcctgaaaaa agttctattt
     1501 tcaggtcgct tgaacagcat ccgatataat cacatcagcg atttcatttt gtaatcactc
     1561 cccaaaacat gaggaaaaaa acaaaattta ttttcttcgt tcagtatatc tatcctaaat
     1621 atgccatcac tccattgagt ttttaatttg tatttcgctc ttgttaaatg tttttctgtt
     1681 tgccgatttc ttttgctaat gtaaaaaata ctctaccact ctcattggtt aaagttttca
     1741 agtattcatc aacatcagta acattaataa caactgtttc atttgtatgt ttattaaatt
     1801 ctaaatcaat aataatattc ccattaacca atatttcata tatatcttct tcttcaggga
     1861 gcatagacaa acaataagca cttgaagctg aaaactcttt tttcagatac tgaagaagaa
     1921 aagtattttt aattaaaata ttattaaatt tatctaatga ttgcttgact tctttagttg
     1981 tccaattttt aacattattc ttcattgtat tctacctggg tcgtagttat taaattacca
     2041 tacttatttg tttggatcgt cataatatta gttggcatgt tgttgttata tttatcatat
     2101 ccaatatcct tattaactcg gatgactctt tcataaattg tatgggaaat gagatttcca
     2161 tcactaagcc tctcaataga tatacgcgtt aatgttggga aagggtttca gacggccttc
     2221 ataatgcgtt cgatttcgtg ccaaaagccg tccggccgac attccaaaac ggcaatcaag
     2281 ccttggtcga tacggcggat ttcggtttcg ttcaatgttt cggtttcggc ggcacgcgca
     2341 ggtgcgaggt aggccatgcg gtcgagcaga tggtagcgga cttcttggcg ttcaaacccg
     2401 tattccgccc aatcttgaat atagacgtcg cgccagccgt atggattaag cggcggctca
     2461 aaagcatgga aaccttgcgg aaaaaatccg atgccgcgca cgatggaaac gggttttacg
     2521 tcaagcggca gaccgtgttg ccgcaaggtt cggatgccgt ctgaagtgtg cggcagaccg
     2581 agttgggcgg tcagtttggc ggcttttccc aacagcgtgt ctttggggtc gagtccgcgc
     2641 atgtcttcga gcctgcccgt gccgccgccg taatatttgc aggtcagctc gatatggtag
     2701 ggcttgccgt taagccttac cacaaaatcc gccgcgcctt gcgtattgcc gtccgaaccg
     2761 gaaaccgtga ggttgtgcgc gagcagttcg gcgtgtggcg cgttggtaaa ccaaaatgcc
     2821 agcagttctt cggcgtaaat gccgaggcgg tgtccgaacg gggcgcgtcg ggtgaggtgt
     2881 tccgtcagcg gcgcgggatc ggcatccaat gccaaaagat agcggaaacc gcgttccccc
     2941 aatagttcgc gcacgcccaa ttcgcaaccg ctttgccaca aaggcggcgc agtcagcagc
     3001 gaggcgaggt cgcggacggg ttggctggtg agcttccacc ataatgcgtc tagggcgtaa
     3061 ttcatgctta tccgaatatc agtcatgccg tctgaaaagc tggaaatgtt ttcagacggc
     3121 cttaatcagt cttttttatg gatacgatgg ctgacggtat tgaaaaaacc gcgcaggata
     3181 tcgatgtctt cggtttgcgt gttggcgcgt ccgaacaggc tctgcatacg gcgcatcaga
     3241 cgctcgccgt tgcggcggtt gaaaaagccg atgtcgttca tcacgctttc catgtgggcg
     3301 accatgcctt tgatttgctc gtgggtcgca gcgtggtctt cttgttgaag atgggtcatg
     3361 ggcgaaccgg tttggctgaa gatttcgtag cacacgacct gcacggcttg ggcgaggttg
     3421 agcgagaaat agtcgggatt gccgttgatg gtcatcagtc ggttgcaggc ttggacttct
     3481 tcgatgctca agccgaaagt ctcgttgccg aaaaccagtg ccactttctc gcctcggttt
     3541 gcggtctgca gtaattcgga taccaaatcg cgcggggttt gcagcggcgc agtaatttcg
     3601 cggcggcggc tggtcagggc gcaggcgatg gtggtgtcgg caagggcttc gtccaaagaa
     3661 gcggcaatgg tggcattttc caaaacgtct gccgcgccgg aagcgaggat gaagctttct
     3721 tccggtaatt taaacgattg aggatgctcc gggtcaaaca cgggcgggtt ttccgtcatc
     3781 ggcgttgcca tcagatttgg ggcgacgatg gtcagtttgt gcagacccat tgttttcatc
     3841 gcgcgcgcgg cagagccgat gttggcggga tggctggtgc gcgtgaggat aatgcggatg
     3901 ttgccgagat agtcgggcag ggcgggtttg agagtagtca tgttcggttt tcggtcggaa
     3961 tggaataggg tataatgcgc cgtctttttc gttcggacgg tcaaatgccg tctgaaatgt
     4021 tctttaacaa cgtcggaaat ttaacgttgc gcctgaagca gacacgcgtc gggcggtatt
     4081 ttacccgatt tggcaggatt taccccaacc aaataaactc cgattttgcc gctccgaagg
     4141 actgcacgtc cgaaccggcg gtatgccacc cttaaggaaa tctgatgaat ccgtttttga
     4201 atacagcctt taaagccgcc cgccgtgccg gtcagatgat gatacgcgcc gcgggcaatc
     4261 tcgatgccgt caaaaccgac agcaaagcat tcaacgattt tgtttccgat gttgaccgca
     4321 attccgaaat catcctggtt gaggctttga aagaagccta tccgcaccac aaaatcactt
     4381 gcgaagaaag cggctcccac ggtaaagccg cagccgaata cgaatggatt atcgatccgc
     4441 tcgacggcac gaccaacttc cttcacggcc atccccaata cgccatctct atggcactcc
     4501 tgcacaaagg cgtgttgcaa gaagctttgg tgtacgcccc cgaacgcaac gacgtttaca
     4561 tggcttcgcg cggcaaaggc gcgttgctca atgaccgccg catccgcgtt tccaaccgca
     4621 tcgaattgaa ccgctgcctg atcggtaccg gctttcctgt tgtcgatcaa agcatgatgg
     4681 acaagtatct ggcgattttg aaagatttct tggcaaaaac cgccggcggc cgtcgtgaag
     4741 gcgcggcttc tttggatttg tgtgctgtgg cgactggtcg ttttgacggc tttttcgagt
     4801 tcaacctcaa accttgggat attgccgcag gcgcattgat tgttcaggaa gcgggcggta
     4861 tcgtaaccga tatgacgggt gaagacggct ggctggaaag cggcgatgtt gtcgctgcca
     4921 atcccaaagt actggcgcaa atgttgaaaa tcatttccgc acacgtttaa atttcagccc
     4981 gctcccggga atgcgggaaa acgggcgggt atgccgtctg aagtatcgcg ccgatatttc
     5041 agacggcatt tttactggca gcgggggcag gaaggtggaa acggctaaat gcccgaaact
     5101 gccgaagatg gaaacccata tctgattaaa cggagaaccc tgtacgattt gtttatagtg
     5161 gattaacaaa aaccagtacg gtgttgcctc gccttagctc aaagagaacg attctctaag
     5221 gtgctgaagc accaagtgaa tgggcaaagc caacgccttt accgcaccga ttaccgtcgt
     5281 caattacgac gaacaagccc tcaataacac cgaagcgcgt actttggtgg atgccgtagc
     5341 gaaaaaagac gcttccgttt ggcagttcgg cggcgaaagc aacacgctga ccggtctgta
     5401 tttcagaggt tatcagcttg actcgcgcca attcagcgtc aacggtttgg caggtatgta
     5461 cggcacgcaa ggcacggcca gcgtgcaagt cggctcggca caactgatta aaggcgcgtc
     5521 caccgccgta aacggcatgg accctgaagg cgcggtatcc ggttccgtca atattgaaac
     5581 caaaaaagcc gccgacgaag acaaccgcaa aatcggtttg ggctggttta gcaacaaccg
     5641 cgcccaaggt acgttcgact tgggtcaacg cttcggcgaa aacaaagaat tcggcgtgcg
     5701 cgccaacggc aaactgcgcc atggcgacac cccgcgccac ggttacagcg aagacaacaa
     5761 agaatttgca ttgaatgctg attatcgcgg cgaaaaagtg cgcgtggcgt tcgactccat
     5821 ctacgcgaaa cgcaaaacca acggcggccg cgcgcgtatg caggatattc aaaacgccgg
     5881 cggccgcttg tttgacgcgc cagacggcaa aatcaacctg ctgctttcgt ggaactggca
     5941 aaacaccgtc ggcgaaacca atatgctgac ctttgaatgg gatgcgtttg acaatgccca
     6001 aattaccggc ggtatcggct acaacaaggc gcgttattac ggcacactga tttccccgac
     6061 cgtttgcggt acaagcggtg cgagcgacca aaccgctacc tgcacaagtg ccaagcaata
     6121 ccacaccggc acggcgagcc tgaccgacca atatttccgc acattgagta tgaacctgac
     6181 cgcacgcggc gaatttgaaa ccggcccggt aacacataac tggagtatgg ctttcgaccg
     6241 cgttatccgc cagcggaaaa ccataaacgg cagcgggaac ggcaacagca agatagaagt
     6301 gaaggcaaac gaaaatattg cgaatcaatt ggcatcgttt acagccgact atcccaattc
     6361 ttgggagaac tctgccaatt tggatgccaa tatcaaagtc aacagcctgg ctttgtccga
     6421 caccttgggc ttcgtggata acaaataccg cctgactttg ggcggacgtt tccaaacagt
     6481 cgaatatacc gacaaaaaat cacagagcgg cgatgccaaa cgtttcagcc cgatgctgat
     6541 ggcggcgtgg gtgccgcaac ctgatttggt tgtgtacggc aactatatgg aagacttgga
     6601 gcctgccgac atcaaaaccg atgattccgg cgaaacgaca atggcgaaac cgcgtgtcag
     6661 ccgccagttt gaagtgggtg tgcgtaaaaa ctggggcaat ttcgtaacca cgttaaacgc
     6721 gttccaaatc aaacgcccgg ctactggcgc ggtcatacaa ctaccaagac aacaaaaggg
     6781 gtaacaacaa cgctgaccta tggcaacaat tccgatttcg cccgctataa ggcgcaaggc
     6841 ggcgtagccg gcgacgagca aggtatggaa cgcaatcgcg gtatcgagtt caatgcctat
     6901 gctaacctgt taaacaaaac cctgcgtccg actttgggcc tgatgtatct gcaatcgacc
     6961 gtgaaagact acccaaattc acgcgatatg ctggttaacg gcgtacaagt cgccaatccg
     7021 cgcgtaattg ccaaggcagg tttggaatgg gatacaccgt ttgccaaagg cttgacttta
     7081 aacagcaatg tttcgtattt cggcaagtct taccaagaca cgcaaaaaca atacgccttc
     7141 ccatcctata ccttggttga tgtaggcgcg cgctacaaaa ccaagctcgg caaaaatacg
     7201 ttgaccgtca gcagctcggt ggaaaatctg ttcaacaaaa actactggca ggtacagcgc
     7261 ggccaatacg accgcagctt cgccgtcgtc ggtctgccgc gtacttactg gctgaaggcg
     7321 gaattggatt tctgatttct ccgcatctgg aaaatgccgt ccgaacaggt ttcagacggc
     7381 atttctttta aagagatatg gcgggatacg cttaaataat gctattctag cgtctttgaa
     7441 acctgcttgt ttgaagtgat gaaaaaacct ttgatttcgg ttgcggcagc attgctcggc
     7501 gttgctttgg gcacgcctta ttatttgggt gtcaaagccg aagaaagctt gacgcagcag
     7561 caaaaaatat tgcaggaaac gggcttcttg accgtcgaat cgcaccaata tgagcgcggc
     7621 tggtttacct ctatggaaac gacggtcatc cgtctgaaac ccgagttgct gaataatgcc
     7681 cgaaaatacc tgccggataa cctgaaaaca gtgttggaac agccggttac gctggttaac
     7741 catatcacgc acggcccttt tgccggcgga tttggcacgc aggcgcacat tgaaaccgag
     7801 ttcaaatacg cgcctgaaac ggaaaaagtt ttggaacgct tttttgggaa acaagctccg
     7861 gtttcccttg ccaataccgt ttattttaac ggcagcggta aaatggaagt cagtgttccc
     7921 gccttcgatt atgaagagct gtcgggcatc aggctgcatt gggaaggatt gacgggaaaa
     7981 acggtttatc aaaaaggttt caaaagctac cgcaacagct atgatgcgcc cttgttcaaa
     8041 atcaagctgg cagacaaagg cgatgccgcg tttgaaaaag cgcatttcga ttcggaaact
     8101 tcagacggta tcaatccgct tgctttgggc agcagtaatc tgaccttgga aaaattctcc
     8161 ttagaatgga aagagggtgt cgattacaac gtcaaattga acgaactggt caatctcgtt
     8221 accgatttgc agattggcgc gtttatcaat cccaacggca gcatcgcacc ttccaaaatc
     8281 gaagtcggca aactggcttt ttcaaccaag accggggaat caggatcatt tatcaacagt
     8341 gaagggcagt tccgttttga cacactggtt tacggcgatg aaaaatatgg cccgctggat
     8401 atccatatcg ctgccgaaca tctcgatgct tctgccttaa ccgtattgaa acgcaagttt
     8461 gcacaaattt ctgccaaaaa aatgactgaa gaacaaatcc gcaatgattt gattactgca
     8521 gtcaaaggcg aggcttccgg attatttacc cataacccgg tgctaaatat caaaactttc
     8581 cgtttcacgc tgccgtcggg aaaaatcgat gtgggcggaa aaatcatgtt taaagacatg
     8641 aagaaggaag atttgaacca attgggactg atgttgaaga aaaccgaggc agacatcaga
     8701 atgagtattc ctcaaaaaat gttggaagat ttggcggtaa gtcaggccgg aaatattttc
     8761 agtgtaaatg ccgaagatga ggcggaagcc agagcaagca ttgcagatat taatgaaaca
     8821 ttgcgcctga tggtggacag tacggttcaa agtatggcaa gggaaaaata tcttacttta
     8881 gacggtaatc agattgatac ggttatttcc cttaaaaaca acgccctgaa gttaaacggg
     8941 aaaacgctgc aaaatgaacc tgatcccgat tttgatgagg gcggtatggt ttcagagccg
     9001 cagcagtaat gggtcaaatg ccgtctgaaa tgttcaggcg gcattttgtt gtattggaaa
     9061 ctgccgtttt caaccattcc tttagataag ccgtgtcgga ttc
//
LOCUS       DAXHTR010000083         9088 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.083, whole genome shotgun sequence.
ACCESSION   DAXHTR010000083 DAXHTR010000000
VERSION     DAXHTR010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.083"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(146..481)
                     /locus_tag="ACFNML_001353"
     CDS             complement(146..481)
                     /locus_tag="ACFNML_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFC6392728.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAATKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            610..1515
                     /gene="mtrA"
                     /locus_tag="ACFNML_001354"
     CDS             610..1515
                     /gene="mtrA"
                     /locus_tag="ACFNML_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HFC6392729.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     MVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1577..2065)
                     /locus_tag="ACFNML_001355"
     CDS             complement(1577..2065)
                     /locus_tag="ACFNML_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6392730.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2212..3113
                     /locus_tag="ACFNML_001356"
                     /pseudo
     CDS             2212..3113
                     /locus_tag="ACFNML_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(3154..4284)
                     /locus_tag="ACFNML_001357"
     CDS             complement(3154..4284)
                     /locus_tag="ACFNML_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFC6392731.1"
                     /translation="MTKHLPLAVLTALLLAACGGSDKPPAEKPAPAENQNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4489..7113
                     /gene="alaS"
                     /locus_tag="ACFNML_001358"
     CDS             4489..7113
                     /gene="alaS"
                     /locus_tag="ACFNML_001358"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFC6392732.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYASDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERIAAVMQHVHSNYEIDLFQDLLKAVARETGAPFSME
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGGAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALVKGGKTLGGEI
                     IFKLYDTYGFPYDLTADICRERNIEPDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVNELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGRHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGEEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELARAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7175..7906
                     /locus_tag="ACFNML_001359"
     CDS             7175..7906
                     /locus_tag="ACFNML_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392733.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8213..>9088
                     /locus_tag="ACFNML_001360"
     CDS             8213..>9088
                     /locus_tag="ACFNML_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IIL restriction-modification enzyme MmeI"
                     /protein_id="HFC6392734.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGVENQWQFPLEALPEYITRGVFDFMFGIEAKVRQI
                     QEEADIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LESCKETDTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWRN"
BASE COUNT         2323 a         2388 c         2170 g         2207 t
ORIGIN      
        1 aatccactat atttcacgct taccccttgt ttctcaaatg ccgtctgaaa taagcggctt
       61 aatatattgt ttacagtatt gggaagcata acagacaaaa tgccgtctga aatattttca
      121 gacggcattt cttatccgaa acggattatt tttgcgtttc aaccgcttcc aatgcacgca
      181 gggcataagt gtaagcggca cccgcattca gggcaatggc ggttgccaat gcacctgcga
      241 tttcgctgtc ggtcgcaccg gctttggtgg cggcggcggc gtgaacactg atgcagctct
      301 cacaacgtgt agtaatggca acggcgatgg caatcagttc gcgtgtttta gcatcaagtg
      361 cctctgcagc tgccgcttgt tccaatgcgc cgtaggcctg cagcatttta ggatgcgcct
      421 tacccagctc gccgaacgat tttttaacca atgcggtatg ttctttccaa tctttaaaca
      481 ttttcttttc ctttctcttg cgtttaaccc tgatacgcgc ttgcgtatct gttttcgatg
      541 tgcgtattat tgcaattatt cagttgtgtt tctcgtttaa tcatctcatt ttatggttca
      601 aaaagattta tggacattct ggacaaactg gtcgatttcg cccaattgac gggcagtgtg
      661 gatgtgcagt gccttttggg cggacaatgg tcggtacggc atgaaacctt gcaacgcgaa
      721 ggattggtac acattgttac atcgggcagc ggctatctct gcatcgacgg cgaaacttcc
      781 ccgcgtccgg tcagtacagg ggatattgta tttttcccgc gcggcttggg tcatgtgttg
      841 agccacgacg gaaaatgcgg agaaagttta caaccggata tgcggcagca cggtgcgttt
      901 acggtcaagc agtgcggcaa cggacaggat atgagcctgt tttgcgcccg tttccgctac
      961 gacacccacg ccgatttgat gaacgggctg cctgaaaccg tttttctgaa cattgcccat
     1021 ccgagtttac agtatgtggt ttcaatgctg caactggaaa gcaaaaaacc tttgacgggg
     1081 acggtttcca tggtcaacgc attgtcgtcc gtcctgctgg tgcttatcct gcgcgcctat
     1141 ctcgaacagg ataaggatgt cgaactctcg ggcgtattga aaggttggca ggacaaacgt
     1201 ttgggacatt taatccaaaa ggtgatagac aaaccggaag acgaatggaa tgtcgacaaa
     1261 atggtggcgg ctgccaatat gtcgcgcgcg caactgatgc gccgtttcaa aagccgggtc
     1321 ggactcagcc cgcacgcctt tgtgaaccat atccgcctgc aaaaaggcgc gttgctgctg
     1381 aaaaaaaacc cggattcggt tttgtcggtc gcactgtcgg taggctttca gtcggaaacg
     1441 cacttcggca aggcgttcaa acggcaatat cacgtttcgc cgggtcaata ccggaaagaa
     1501 ggcgggcaaa aataaatcgg ggcttcaaac gcaaatgccg tctgaaaagg ctttcataca
     1561 gcatttgcgt accgcgtcat ttcaagggct gcatcttcat cacttccatc aaaaagttgg
     1621 taaatgcggg gttgttgggt ttgacatcca tatttttcca acgctgctgc cagccgcgca
     1681 aggcattctg gatatacagc ttggactgtt ccgtattgat tgcgccccgc tggctgtcta
     1741 tcgccgaacg caggtagatt tcatacatac tgtcatcgac ggcattgcgt ccgaccaggc
     1801 gttttctgaa gttgttcaga tattgcgccg cctgaacctt ggtcatttta ccgataccca
     1861 cctgatagcc caagcgcgtc gcttcatcgc tgattttggc aacatccgtc caatgcgaag
     1921 aggcaaggcg gaaacctttt gcaggtgctt ccgttttgac ggtattgata ggattcacgg
     1981 ggatttccgt caatgtgggc acataaatag actggcagcc ggaaagaact gccgcaatgg
     2041 aaagagggat aaggtatttt ttcatgcccc cattataatc aagtttgcct tgagaaaaca
     2101 aattgttcgg caagaaaaat aaaatttcgg catcagaagc aggcaaaaac acattccaca
     2161 agccttgccg caaggtttac aatccgaccg tccttatcgc aacgaccgtt tatggatacc
     2221 gcaaaaaaag acattttagg atcgggctgg atgctggtgg cggcggcctg ctttaccatt
     2281 atgaacgtat tgattaaaga ggcatcggca aaatttgccc tcggcagcgg cgaattggtc
     2341 ttttggcgca tgctgttttc aaccgttgcg ctcggggctg ccgccgtatt gcgtcgggac
     2401 accttccgca cgccccattg gaaaaaccac ttaaaccgca gtatggtcgg gacgggggcg
     2461 atgctgctgc tgttttacgc ggtaacgcat ctgcctttgg ccactggcgt taccctgagt
     2521 tacacctcgt cgattttttg gcggtatttt ccttcctgat tttgaaagaa cggatttccg
     2581 tttacacgca ggcggtgctg ctccttggtt ttgccggcgt ggtattgctg cttaatccct
     2641 cgttccgcag cggtcaggaa acggcggcac tcgccgggct ggcgggcggc gcgatgtccg
     2701 gctgggcgta tttgaaagtg cgcgaactgt ctttggcggg cgaacccggc tggcgcgtcg
     2761 tgttttacct ttccgtgaca ggtgtggcga tgtcgtcggt ttgggcgacg ctgaccggct
     2821 ggcacaccct gtcctttcca tcggcagttt atctgtcgtg catcggcgtg tccgcgctga
     2881 ttgcccaact gtcgatgacg cgcgcctaca aagtcggcga caaattcacg gttgcctcgc
     2941 tttcctatat gaccgtcgtt ttttccgctc tgtctgccgc attttttctg ggcgaagagc
     3001 ttttctggca ggaaatactc ggtatgtgca tcatcatcct cagcggtatt ttgagcagca
     3061 tccgccccac tgccttcaaa cagcggctgc aatccctgtt ccgccaaaga taaaaaatgc
     3121 cgtccgaaca tccttcagac ggcatatcgg gctttatttc cccgccttca catcctgcca
     3181 ctggcgcacc ataaacttca atgccgccgg ctggataggc accatgataa agctgttttt
     3241 caaatcctcc tcggttggga aaatcgtatt gtcgttttta aattcgtctt ccatcagctc
     3301 acgcgcaggc ttgctcgaag gcgcgtaagt aacgaaattg ccgtttttcg ccgacacttc
     3361 cgggtcgagg aagtcgttga tgtatttgtg cgcgttggcg acgtttttcg catctttcgg
     3421 aatcacgaaa gaatccaccc aaatccccac gccctctttg ggcatcatca cgcggatttt
     3481 ttccttgccg cccgcttctt cggcacggcg tttggcgatg ttcaaatcgc cgccgaaacc
     3541 gattgttacg caggtatcgc cgcgcgccaa atcatcgata aagccggacg aagtaaagcg
     3601 tttgatattg gggcggtttt tcttgagtag ggcggttgcc tccctgatgt cttccgtatt
     3661 gctgctgttc gggtttttac ccaaatagtt caacaccata ggatagattt ccgccgcgct
     3721 gtccaaatag ctgatgccgc attgcttgag tttggacgtg tattcggggt cgaacaccaa
     3781 atcccactgg ttgtccggca gcttgtccgt acccaaagcc tttttcacgc gttcggtatt
     3841 gatggcgaag gtatttgtcc cccaataaaa cggcacggcg tattcgtggc cgggatcgac
     3901 cccgtccatc agcctcatca tttcggggtt gaggtgttta taattgggaa tcagcgactt
     3961 atcgattttc tgatacgcac ctgccttaat ctgcctgccc acaaacgcat tggacggcgc
     4021 gacaatgtcg taaccagatt ttccggtcag caccttgctt tccagcgttt catcgctgtc
     4081 gtacacatca taagtaacct tgatgccgtt tttcttttca aaatcggcaa cggtttccgg
     4141 atcgacatat tccgaccagt tgtaaatttt caatacgttt tggttttccg ccggtgccgg
     4201 tttttcggca ggcggtttgt ccgaaccgcc gcacgctgca agcagcaaag cagtcaggac
     4261 ggccaggggc agatgtttgg tcattatcat tccttgcata tcgggttgga gaaagcggcc
     4321 attatagccg atattggcaa cagggcttca gacggcattc aaaatcccgc cacactcttc
     4381 cgaaaaccgc cgcttccata gctagaaaca gggatttgcg gtaagatacc gccgttcgtt
     4441 ttccctgctt ttaccatgac aagacatttg agagacattg aaaaaattat gaaaacctcc
     4501 gaactgcgcc aaaaattcct aaaatttttt gaaaccaaag gccacaccgt cgtccgctct
     4561 tccagcctcg tgccgcacga cgacccgacc ctgctgttta ccaacgcggg catgaaccag
     4621 tttaaagacg tattcttagg tttcgacaaa cgcccgtaca gccgcgccac caccgcgcaa
     4681 aaatgcgtac gcgcaggcgg caaacacaac gacttggaaa acgtcggcta caccgcccgc
     4741 caccacacct tctttgaaat gatgggcaac ttctccttcg gcgactactt caaacgcgac
     4801 gccatccact tcgcttggga atttctgact tcccccgaat ggctcaacat ccctaaagac
     4861 aaactgttgg cgaccgttta cgcggaagac gacgaagcct acaacatctg gttgaacgaa
     4921 atcggtatgc cgtccgagcg catcgtccgc atcggcgaca acaaaggcgc gaaatacgca
     4981 tccgacaact tctggcaaat gggcgacacc ggcccttgcg gcccctgctc cgaaattttc
     5041 tacgaccacg gcgaagaaat ctggggcggc attcccggca gtcccgaaga agacggcgac
     5101 cgctggatcg aaatttggaa ctgcgtattt atgcagttca accgcgacga acaaggcaat
     5161 atgaacccgc ttcccaaacc ttccgtcgat accggtatgg gcttggaacg catagccgcc
     5221 gtcatgcagc atgttcacag caactacgaa atcgacttgt tccaagacct gctcaaagcc
     5281 gttgcccgcg aaaccggcgc gccgttcagt atggaagaac ccagcctgaa agtcatcgcc
     5341 gaccacatcc gctcctgctc gttcctgatt gcagacggcg tcttgccttc caacgaaggc
     5401 cgcggctacg tattgcgccg cattatccgc cgcgccgtgc gccacggtta caaactgggt
     5461 caaagcaaac cgttcttcca caaactcgtt gccgatttgg tcaaagagat gggcggtgcc
     5521 taccctgaat tgaaagaaaa acaagcccaa atcgaagaag cattgaaaaa cgaagaaagc
     5581 cgttttgccc aaacgctgga aaccggtatg gctttgttgg aaaacgcgct ggtcaaaggc
     5641 ggcaaaacac tcggcggcga aatcatcttc aaactctacg atacctacgg tttcccatac
     5701 gacttgactg ccgacatctg ccgcgaacgc aatatcgaac cggacgaagc aggcttcgag
     5761 cgcgaaatgg aagcccaacg cgcacgcgca cgcgccgccc aaagcttcaa agccaacgcc
     5821 caactgcctt atgacggtca agacaccgag tttaaaggtt atagcgaacg ccaaaccgaa
     5881 tccaaagtcc tcgccctcta caaagacggc gagcaagtca acgaattgaa cgaaggcgac
     5941 agcggcgcag tcgtcatcga ctttaccccg ttctatgcag aatccggcgg ccaagtcggc
     6001 gatgtcggct atatcttctc aggcgaaaac cgctttgaag tacgcgatac ccaaaaaatc
     6061 aaagcggccg tattcggtca attcggcgta caaacttcag gccgtctgaa agtcggcgac
     6121 agcgttaccg ccaaagtgga cgacgaaatc cgcaatgcca atatgcgcaa ccacagcgca
     6181 acccacttga tgcacaaagc cctgcgcgat gtattgggca gacacgtcga acaaaaaggc
     6241 tctttggtta ccgccgaatc cacccgtttc gacatttccc atccccaagc ggtaactgcc
     6301 gaagaaattg ccgaagtaga acgccgcgtc aacgaagcca ttttggcgaa cgttgccgtc
     6361 aatgcagcca ttatgagcat ggaagacgcg caaaaaaccg gcgcgatgat gctcttcggc
     6421 gaaaaatacg gcgaagaagt gcgcgtactg caaatgggcg gtttctctac cgaattgtgc
     6481 ggcggcacac acgtttcacg caccggcgac atcggcctct tcaaaatcat cagcgaaggc
     6541 ggtattgccg caggcgtgcg ccgtatcgaa gccatcaccg gcctgaacgc actcaaatgg
     6601 gcgcaagagc aagagcgttt ggtgaaagac attattgccg aaaccaaagc ccaaaccgaa
     6661 aaagacgtac tggcaaaaat ccaagcaggc gcggcacacg ccaaagcatt ggaaaaagaa
     6721 ttggcacgcg ccaaagccga actcgccgtc cacgcaggcg ccaaactctt ggacgatgca
     6781 aaagacttgg gcgcagccaa actcgttgcc gcccaaatcg aagccgacgc agccgccctg
     6841 cgcgaaatcg ttaccgattt aaccggtaaa tccgacaacg ccgtgattct tttagcggca
     6901 gtaaacgacg gcaaagtctc cctgtgcgcc ggcgtatcca aaccgttgac cggcaaagtg
     6961 aaagcaggcg atctggttaa atttgcagcc gaacaagtcg gcggcaaagg aggcggccgt
     7021 cccgacttgg cgcaggccgg cggcacggat gccggcaaat tgcccgaaat gctggtaagc
     7081 gtggaaagct ggctttgtca gaaattaagt taaatttaaa tcttaataag ccatttgagt
     7141 cttgttcaaa tggcttatat tttggattgt tagaatgaat actaaaacag agttacaaaa
     7201 acttttagaa gaggatatat caactttaac agaaacacta atttgtgcag atgctcttcc
     7261 tccaagatat gtacgtagca ttgcaactcc tattgtccgt cgatggttaa ttgacaaaca
     7321 attaaatatt ctcgcaaaag aaataggttt aactatagaa ttgcccatcc ttgatacaag
     7381 tttggtattt gaaaaattgt caaccttaga gaataaagtt aatttttata tggctggtgg
     7441 agtttattta ggcggagaat ttattagctc tatatatcat tcatcacaag aattttctgg
     7501 tgaaccaata atctatgctg aaccaaatat aattttatgt cctgctgaaa aatttttaac
     7561 tttaaaacga gttttccata atggaaatat ttttaatatg aatcagataa ttactttttt
     7621 atccaataaa caaggaggag tacattttga taaaaattac gataaatata aaacttggca
     7681 agttgctata gaaaaagcag caaatttttt aaaactcggt aatccatata atgaagataa
     7741 attatctctt agtgaagaac atgatactat tttagtagtt ttaccattag aaaaaggata
     7801 tgaatggaat tgtcttgaga tagaagtact atccgctgct caatctctag caaatatcta
     7861 ttgcaacaaa gttcgcctca tagatggtca tgtttggaag gaataaaaga aaaagcatag
     7921 ttggtaattt ttagttacgt agggtgggct tcagcccacc gttttttcat ttttgattgt
     7981 tgattggtgg gctaaagccc accctacggt tactaaaatc ccgtcattac ctcgcaggcg
     8041 ggaatccggt ttgttcggtt tcggtttttt agggacagtg ccgcaacatt aaatttccag
     8101 attcccactt tcgcacaaat aacagcgcaa atagggcaaa gataaggata tgggtttttt
     8161 tctttgaatt tactatacaa ttcgcccgtc cccgttatag aaacccaagc cgatgaaaac
     8221 cctgctccaa ctccaaaccg ccgcacaaaa cttcgccgcc tactacaaag accaaaccga
     8281 cgaacgccgc gagaaagaca ccttctggaa cgaatttttc gccattttcg gcatcgaccg
     8341 caaaaacgtc gcccacttcg aataccccgt caaagaccct gccgacaaca cccaattcgt
     8401 cgatatattt tgggagggca tcttccttgc cgaacacaaa tccgccaaca aaaacctgac
     8461 caaggccaaa gagcaggcgg agcgttattt gcaggaaatc gggcgcacca agccctctgc
     8521 gctgcccgaa tattacgccg tcagcgattt tgcccatttc cacctttatc gccgcgtacc
     8581 tgaagaaggc gtggaaaacc aatggcagtt ccctttggaa gcattgcccg aatacatcac
     8641 gcgcggcgtt ttcgacttca tgttcggcat cgaagccaaa gtccgccaaa ttcaagaaga
     8701 agccgatatt caagcggcgg cggccatcgg caggctgcac gacgcgctca aagaagaagg
     8761 catttacgaa gaacacgagc tgcgcctctt catcacgcgc ctgcttttcc tcttttttgc
     8821 cgacgacagc gccgttttcc agcgcaacta tctctttcaa gactttttag aaagctgcaa
     8881 agaaaccgac acgctcggcg acaagctcaa ccaactcttt gaattcctca acacacccga
     8941 ccaaaagcgc agcaagaccc aaagcgaaaa atttaaaggt ttcgaatacg tcaacggcgg
     9001 cctgtttaaa gaacgcctgc gcaccttcga cttcaccgcc aagcagcacc gcgccttaat
     9061 cgactgcggc aatttcgact ggcgcaac
//
LOCUS       DAXHTR010000084         9087 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.084, whole genome shotgun sequence.
ACCESSION   DAXHTR010000084 DAXHTR010000000
VERSION     DAXHTR010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9087
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.084"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            216..332
                     /locus_tag="ACFNML_001361"
                     /pseudo
     CDS             216..332
                     /locus_tag="ACFNML_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            545..2080
                     /locus_tag="ACFNML_001362"
     CDS             545..2080
                     /locus_tag="ACFNML_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6392735.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLNIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     DEK"
     gene            2077..2169
                     /locus_tag="ACFNML_001363"
     CDS             2077..2169
                     /locus_tag="ACFNML_001363"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HFC6392736.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(2229..3449)
                     /gene="lysA"
                     /locus_tag="ACFNML_001364"
     CDS             complement(2229..3449)
                     /gene="lysA"
                     /locus_tag="ACFNML_001364"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFC6392737.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAELS
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEAE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTAPVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDTLVA
                     EGIVLEHLDLGGGVGIVYQDESVPDLGAYAQAVQKLIGTRRLKLILEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKNIATLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGGGYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(3460..3630)
                     /locus_tag="ACFNML_001365"
     CDS             complement(3460..3630)
                     /locus_tag="ACFNML_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFC6392738.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            3701..4024
                     /gene="cyaY"
                     /locus_tag="ACFNML_001366"
     CDS             3701..4024
                     /gene="cyaY"
                     /locus_tag="ACFNML_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFC6392739.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAGD
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            4051..5067
                     /locus_tag="ACFNML_001367"
     CDS             4051..5067
                     /locus_tag="ACFNML_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFC6392740.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTVLLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAEPVTKAESHKVSVAIAVV
                     VIFGTLAIFTYPLFYTWSQHLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSNGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTQASAIKKAGLKPFVLGILTYLWLVIGGFLVNYGI
                     SKLI"
     gene            5093..5512
                     /locus_tag="ACFNML_001368"
     CDS             5093..5512
                     /locus_tag="ACFNML_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HFC6392741.1"
                     /translation="MAQLPLYRTAEIGNFTVGTPKVLESFSKHIPYGVVFEDDGDTGY
                     FYAASKDGILDALHIYNVEDVSDKHIPNHVLILWDDACTLAALCINDYIHAVYDFVAQ
                     AGYCRNGFPETGGEWVKVENRVLDDELLDKILSRKST"
     gene            5534..6061
                     /gene="luxS"
                     /locus_tag="ACFNML_001369"
     CDS             5534..6061
                     /gene="luxS"
                     /locus_tag="ACFNML_001369"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFC6392742.1"
                     /translation="MPLLDSFKVDHTRMHAPAVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     TWLASMQDVLNIKDQSKIPELNEYQCGTYQMHSLAEAQQIAQNVLARKVAVNKNEELT
                     LDEGLLNAQSAKNAV"
     gene            6186..9002
                     /gene="polA"
                     /locus_tag="ACFNML_001370"
     CDS             6186..9002
                     /gene="polA"
                     /locus_tag="ACFNML_001370"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFC6392743.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLSAPDGAPTGAMYGVLNM
                     LRRLRADYVHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLMG
                     WPVLVIGQVEADDVIGTLAKQGAEHGLRVIVSTGDKDMAQLVDERVTLVNTMSSETLD
                     IEGVKAKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLQGVIEHAS
                     EIKGKVGENLQAALPQLPLSYDLVTIKTDVDLHAELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESNMNTGSTDDLFGSDSIGEQAALNAEMPFEKQAEKATAPEKLDYQA
                     VTTEAQFAALLDKLSRADTIGIDTETTSLDAMNASLVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYGIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQISFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDAKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPWDNRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGFVETLFGRRLYLPDIHNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVSRWLSECEASPWDELLQSKLIMQVHDELVLEVVETELDFVKEKLPQIMAK
                     VGGGLLDVPLVAEVGVGENWEEAH"
BASE COUNT         2172 a         2323 c         2368 g         2224 t
ORIGIN      
        1 tcagaaccac attaacggca ttgagaactt ttggaatcag tcaaaacgcg tcttgcgaaa
       61 atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt ataaatagta
      121 cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
      181 agacaacgct gtactggttt aaatttaatc cactatacaa cggaatcgat tgcaaatctt
      241 tcccactgtt cttgaaagaa tgcgaatttc gatttaactt cggcacaccg tctcaacagc
      301 ttaaaatcct gcgggattgg tgtgggattt agggctaatc tagtacagcc cctaaaattt
      361 ttcgttttca aaccttcacc gcttgccatc agcgttaaat ttttttacga taagcacata
      421 gattgtaaac aatcggccac aagccggttt gttttttcag aagacattat ccctgtcaga
      481 cgctgtttct atatatttcg cctataatgg cttgttttta ataaataatt caagaggtat
      541 caacgtgtct gattccaaga cgaaagaacg cgccacattc ggcacgcgcc gcgcgtttat
      601 gattgccgcc atcgggtccg ccgtcggctt gggcaatatt tggcgtttcc cctatattgc
      661 ttttgaaaac ggcggcggcg cgttcatcct gccctatctg gtcgcgcttc tgacggcggg
      721 catcccgctg ctgctgctcg attatgccat cggccaccgt taccgtggtt ctgcgccctt
      781 ggctttccgc cgcctcggac gatggtttga gccggtcggc tggtggaacg tgatgaccaa
      841 tatcgtcatc tgcatctatt acgcggtaat tatcggttgg gcggcaagct atacctatta
      901 ttcggtcaac gccgcctggg gtgcggatcc gcagggtttt ttctttaagg acttcctgca
      961 aatggcgggc ccggaagcct tgggtttgga ttttgtcggc aaagtcgccg gtcctttggc
     1021 gggcgtgtgg gtttttaccg ccgccattat ggcgttgggc gtgcaaaagg gcgtggcgcg
     1081 cgcctcgtcg ttctttatgc cgctgctttt ggtgatgttt ttgattatgg tcggcatttc
     1141 actaaccctg ccgggtgcgg caaagggctt ggacgcattg tttacgcccg actggtcgaa
     1201 actcgccgat tccaaggtct gggtggcggc atacgggcag attttctttt cgctttccat
     1261 ctgcttcggc attatggtta cctattcttc ttatttgaag aaaaaaaccg acttgggcgg
     1321 aacggggctg gtggtcggtt ttgccaacag cagctttgaa ctgctcgcgg gcatcggcgt
     1381 gtttgccgca ttgggcttta tggcgcaggc gggcggtaag gcggtcaacg aggttgcctc
     1441 aggcggcatc ggtttggcgt ttatcgcctt tccgaccatt atcaaccagg caccgatggg
     1501 ctggctgatc ggcatattgt ttttcggttc gctggtattc gccggcgtta cgtcgatgat
     1561 ttccatcctt gaagtgattg tggcggcgat tcaggacaag ctgaacatcg ggcgcgtcaa
     1621 cgccacgctg ctggtctgca ttccgatggg cattgtttcc acgctgctgt tcggtacggc
     1681 gacggggctg ccggttttgg acgtgatgga caaattcgtc aacacctacg gcattgttgc
     1741 cgccggcttt gtttatgttg ccgccatcat catcagcggc aggctgccgg aattacgcaa
     1801 gcacctgaac gccttgtcct ccatccgcat cggcggcttg tggacggtct gcgtcgtggt
     1861 taccgtcgtg atgctcggct atatgctgtt taaagatacc agcggcctga tggagaaaaa
     1921 ttacgaaggt tatccggatg gtttcctcaa tattttcggt tgggggatgt cggcggcgtt
     1981 ggtcgtgttc gggctgctgc tgtcgttgct gccttggaaa cacggtcagg atttcaacgt
     2041 caaagacgaa cacgaacatg aacaaggaga cgaaaaatga gtacttccgc cattgtgatg
     2101 atgattgtct caatcgtgat aatctgggga gggctgctgc tttccctgtt aaggctgccg
     2161 aacgagtaag cctttagagc gttaaaaatg ccgtctgaac tgtttcagac ggcattttga
     2221 tgttcggctt acaggcaggc gagttcgttt gccatttgct gttccaaggt ttcgcgccgg
     2281 cggatgagtc ggtatccgcc gccgtcaacc aatacctccg ccgcacggtt gcgcgcgttg
     2341 taattgctcg ccatgctggc accgtatgcg cccgcgctgc ggataagcag caaatcgcct
     2401 tcttcgcagg cgatggtgcg gtctttgccg aggaagtcgc cggtttcaca aatcgggccg
     2461 acgatgttgg cggtcagcgt cgcgatgttt ttggtttcga ccgcttcgat gtggtggtag
     2521 gcatcataaa gtgccggacg cattaaatcg ttcatcgccg catcgaccat cacaaagttt
     2581 ttctcttcgc cgtgtttgac gaattcaacg cgcgtcaata acgcacctgc gttaccgacc
     2641 aagctacgac ctggttcgag gatgagtttc agacggcgtg tgccgatcag tttttgaacc
     2701 gcttgggcat atgcgcccaa atcggggacg ctttcgtctt gataaacgat gccgacaccg
     2761 ccgcccaagt ccaaatgttc caaaacaatg ccttcggcga caagagtgtc aaccaaaatc
     2821 aaaatgcgtt cgcaggcttc gaccagcggg cttaagtcgg ttagttggga gccgatgtgg
     2881 cagtcgatgc cgatgatttt caaattgggc tgttgtgcgg catggcggta ggcttcgagc
     2941 gcgtcggcgt aggcgatacc gaatttgttg gctttcaaac ctgtggagat gtaaggatgg
     3001 gttttcgcat cgacatcggg attgacgcgc agggagacgg gcgcggtttt acccaaacgc
     3061 gcggcaactt tctgaatacg gtcgatttcg gggatgcttt ccatattgaa gcatttcacg
     3121 cctgcattca gcgcgaactc gatttccgcc tcgcttttgc ctacgcctga aaatatggtt
     3181 tttgccgcgt cgccgcctgc cgccaaaacg cgtgccaatt cgccgccgga cacaatgtca
     3241 aaaccgctcc ccaaagaagc aaagtgtttg atgatgctca ggttgccatt ggctttaacc
     3301 gcatagcaga cgagcgggga gagctcggca aaggcagttt ggtagtgttc aaatgcttcg
     3361 gtcagcgcgg attggctgta cacataaagc ggtgtgccga atgcttcggc aaggcggggg
     3421 taggggactt gttcgcaaaa tagggtcata ttttcgtttt catttttggg ttggaggggc
     3481 ggattgcggt ttgccttgaa gctgcaaacc ggtttggatt acgccgaaac gcgccttgtc
     3541 gccttctttg ggcaggtaga ggtcgccttt gtaaccgcag gccgagagca ggagggcggt
     3601 tgccgccgca aaaaatacgc cgtatttcat cggtaaactt ccttcataag cgcgaatgtg
     3661 gcaagattcg gcatcttaaa caaaaaacac gcaaaaagct atgatgaccg aaagcgagtt
     3721 tatccgcgcg agcgaagcat tatttgaaca catcgaagac caaatcgacg aaaacggctg
     3781 ggatttcgac tgccggtttg ccggaaacgt cctgaccatc gaagccggag acggcacgca
     3841 aatcatcgtc aaccgccaca cgcccaacca agaattgtgg attgccgcaa aaagcggcgg
     3901 ctaccatttc gccgaacaaa acggcaaatg gctggcaaca cgcgacagcc gcgatttcta
     3961 cgacgtttta aacgaagcac tgagcgcggc ttcgggcgaa gcggtagaga ttgaggaatt
     4021 gtgatttggg tgttatcacg gagagaaaaa atgaacacac gtccctttta tttcggactt
     4081 atatttatcg cgattatcgc tatacttgct aactatttag gaaacactga tttttcccat
     4141 cattatcata tcagtgcttt aattattgct atcttgctgg gaatggcaat cggcaatacc
     4201 atttatccgc aattttcaac acaagtggaa aaaggcgttt tgtttgccaa aggcgcgctt
     4261 cttcgcactg gcattgtgct gtatggtttc cgcctcactt ttggcgatat tgccgatgta
     4321 ggattaaatg ctgtcgttac cgatgcaatc atgctaattt caaccttctt ttttaccgta
     4381 cttttaggca ttcgttatct aaaaatggat aaacaattgg tttatctcac tggggcaggt
     4441 tgcagcattt gcggtgcggc agcagtgatg gcggcagagc ctgttaccaa agcagaatcc
     4501 cataaagttt cagtggcgat tgccgtagtg gtcattttcg ggacgcttgc tatttttact
     4561 taccccttgt tctacacgtg gtcacaacat ttaattaacg cccatcaatt cggtatttat
     4621 gttggttcta gtgtacacga agtggctcaa gtttatgcga ttggggaaaa tattgatcct
     4681 atcgtggcga atactgccgt catttccaaa atgatccgag tgatgatgct cgcccccttt
     4741 ttattaatgc tttcttggtt attaacacgt agtaatggag tatcagaaaa tacatcacac
     4801 aaaattacaa ttccttggtt tgctgtactt tttattggtg ttgccatttt taattctttt
     4861 gatttattac caaaagaact cgtgaaatta ttcgttgaaa tcgattcttt cttattaatt
     4921 tcatcaatgg ctgcgcttgg cttaacgacg caagcaagcg caatcaaaaa ggcaggatta
     4981 aaaccgtttg ttttaggaat actaacttat ttatggctag tgattggtgg atttttagtg
     5041 aactatggaa tatcaaaatt aatataaaat tcactaaaga gagcgttacc caatggcaca
     5101 attaccgcta tatcggactg ccgaaatcgg aaattttact gtcggcacgc ctaaagtttt
     5161 agaatcattt tccaaacata tcccttatgg cgtcgtcttt gaagacgacg gcgacacagg
     5221 ttacttttat gccgcctcga aagatgggat tttggatgct ttgcatatct acaatgtcga
     5281 agatgtatcc gacaaacata tccccaatca tgtcttgatc ttatgggacg atgcctgcac
     5341 tttggcagct ttgtgcatca acgactacat ccatgccgtc tatgattttg tcgcgcaggc
     5401 aggatattgc cgcaacggct tccctgaaac aggcggcgaa tgggtgaaag tcgaaaaccg
     5461 cgtcttggac gatgaattgc tggacaaaat cttatcccga aaatctacat aacctactca
     5521 aaaggataac aaaatgcccc tgttagacag tttcaaagtc gaccacaccc gtatgcacgc
     5581 ccctgccgta cgcgtggcga aaaccatgac cacgcccaaa ggcgatacca ttaccgtatt
     5641 cgacctgcgc ttttgcgttc ctaacaaaga aatcctgcct gaaaaaggca tacacacgct
     5701 ggagcatttg ttcgcaggct ttatgcgcga ccacttgaac ggcaacggcg tggaaatcat
     5761 cgacatttcc ccgatgggct gccgcaccgg tttctacatg agccttatcg gcacgccttc
     5821 cgaacagcag gtcgccgata cgtggctggc ttcgatgcag gatgttttga atatcaaaga
     5881 ccaaagcaaa atccccgagt tgaacgaata ccaatgcggc acttatcaaa tgcactcgct
     5941 cgccgaagcg cagcaaatcg cgcaaaacgt gttggcgcgc aaagtggcgg tgaacaaaaa
     6001 tgaagagttg acgctggatg aagggctgct gaacgcccaa tccgccaaaa atgccgtctg
     6061 aacaagggtt tcagacggca tttgcctttt ccgttataat ccggggttgt gcgggggcgg
     6121 gttttaagcc ggcatcgtcc ttccctattt ttttctgtcc cttatcggtt ttaagcgggt
     6181 tttttatgtc caacagacct acactcctcc tcgttgacgg ctcttcctat ctctaccgcg
     6241 cgtatcacgc gatggggcag aatttgtctg cgcctgacgg tgcgccgacg ggggcgatgt
     6301 atggcgtgtt gaacatgctg cgtcggttgc gggcggatta tgtgcatgat tattgcgcgg
     6361 tggtttttga tgcgaaaggc aaaaatttcc gccatcaaat gtttgaagaa tacaaggcga
     6421 cgcgcccgcc aatgccggac gatttgcgtc ctcaagcgga agccttgccg gatttggtgc
     6481 ggttgatggg ctggccggta ttggtgattg ggcaggtgga ggcggacgat gtgatcggca
     6541 cgctggcgaa acagggggcg gaacatggtt tgcgagtcat tgtttcgacc ggcgataagg
     6601 acatggcgca gttggtggat gagcgcgtta cgctggtgaa cacgatgagc agcgaaacgc
     6661 tggacattga aggcgtgaag gcaaaattcg gcgtgcgccc cgaccaaatc cgcgattatc
     6721 tcgcgctgat gggcgacaag gtggacaacg tgccgggcgt ggaaaaatgc ggcccgaaaa
     6781 cggcggtgaa gtggctggaa gcctacggct cgttgcaagg cgtgatagaa cacgcttcgg
     6841 aaatcaaggg caaagtgggc gaaaacctgc aagccgcgct gccccaactg ccgctgtcgt
     6901 atgatttggt cacgattaaa accgatgtgg acttgcacgc cgagctttca gacggcatcg
     6961 aaagcctgcg ccgtactacg ccgaaatggg cgcagctggt tgtcgatttc aaacgctggg
     7021 gcttccgcac ctggctgaaa gaagcggaat caaacatgaa taccggctcg accgatgatt
     7081 tgttcggcag cgacagcatc ggcgagcagg cggctttgaa tgcggaaatg ccgtttgaaa
     7141 aacaagccga aaaagccacc gcccccgaaa aactggatta tcaagccgtt accaccgaag
     7201 cgcagtttgc cgctttgttg gacaaactgt cgcgggcgga cacaatcggc atcgatacgg
     7261 aaaccacgtc attagacgcg atgaacgcct cgctggtcgg catcagcatc gctttccaag
     7321 caggcgaagc ggtttacatc cccgtaggac acagcctgac cgccgcgccc gaacagcttg
     7381 atttgcaaga cgtattaggc cgtctgaaac cgcatttgga aaaccccgcc ctgaagaaaa
     7441 tcgggcaaaa cctcaaatac gaccaacacg ttttcgctaa ctacggcatc gccctgaacg
     7501 gcattgccgg cgacgctatg ctcgcttcct acatcatcga aagccatctt ggacacggct
     7561 tggacgaatt gtccgaacgt tggctgggct tggaaaccat tacctacgaa tcattgtgcg
     7621 gtaaaggcgc gaagcaaatc agttttgccg atgtcgccat cgggcaggcg accgaatacg
     7681 ccgcccaaga cgccgatttc gccttgcgcc tcgaagcgca cctgcgcgcg caaatggacg
     7741 ccaaacagct tgaaatgtat gaaaaaatgg agctgcccgt cgcgcaagta ttgtttgaaa
     7801 tggaacgcaa cggtgtgcaa atcgaccgcg ccgaactcgc ccgccaaagc gcggaactcg
     7861 gcgccgagct gatgaagctc gaacaacaag cctacgccgc cgcaggccag ccgttcaacc
     7921 tcaattcgcc caaacagctg caagaaatcc tgttcgacaa aatgggcatc ccgacaaaag
     7981 ggctgaaaaa aactgccaaa ggcggcattt ccaccaacga agccgtgctc gaacagctcg
     8041 cgcccgacta ccccctgccc aaaatcatcc tgcaaaaccg cagcctggcg aaactcaaat
     8101 ccacctacac cgacaaactg cccgaaatga tttctccctg ggataaccgc gtgcatacca
     8161 cctacgccca agccgtcgcc attacaggac gcttggcaag caacaacccc aacctgcaaa
     8221 acatccccat ccgcaccgcc gaaggccgcc gcgtacgtcg cgcctttacc gcaccgcaag
     8281 gcagcgtcat cgtttccgcc gactattccc aaatcgagct gcgcatcatg gcgcacctct
     8341 ctggcgacaa aaccctcatc gctgcgttcc aaaacggcga agacgtacac cgccgcaccg
     8401 ccgccgaagt gttcggcatc gcccccgaaa acgtctcgtc cgaacaacgc cgctacgcca
     8461 aaaccatcaa cttcggcctc atttacggca tgggccaata cggcctcgcc aaatcattgg
     8521 gcatcgacaa catctccgcc aaaaacttta tcgaccgcta cttcgcccgc tatcccggcg
     8581 tcgccgaata catgcagcgc accaaagaac aagccgccgc acaaggcttt gtcgaaaccc
     8641 tgttcggcag aaggctctac ctgcccgaca tccacaacaa aaacgccaac gcccgcgccg
     8701 gagccgaacg cgccgccatc aacgccccca tgcaaggcac cgcctccgac ctcatcaaac
     8761 gcgccatgat agacgtgtcc cgctggcttt cagagtgcga agcctccccg tgggacgaac
     8821 tcttacaaag caaactgatt atgcaggtgc atgacgaact ggtgctggaa gtcgttgaaa
     8881 ccgaactgga ttttgtcaaa gaaaaactgc cgcagattat ggcgaaagtg ggcggcggat
     8941 tattggatgt accgctggtg gctgaggttg gcgtagggga gaattgggaa gaggcacatt
     9001 gattgaaagg tgttatatgc tatctttatt taaataaaat ttaatttttg gtatattttt
     9061 tctaaatgtt cctatagtat agtggat
//
LOCUS       DAXHTR010000085         9037 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.085, whole genome shotgun sequence.
ACCESSION   DAXHTR010000085 DAXHTR010000000
VERSION     DAXHTR010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.085"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..132)
                     /locus_tag="ACFNML_001371"
     CDS             complement(<1..132)
                     /locus_tag="ACFNML_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HFC6392744.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     "
     gene            371..1270
                     /gene="crgA"
                     /locus_tag="ACFNML_001372"
     CDS             371..1270
                     /gene="crgA"
                     /locus_tag="ACFNML_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HFC6392745.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAMP
                     MVLHLLAPLATKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(1498..4713)
                     /gene="carB"
                     /locus_tag="ACFNML_001373"
     CDS             complement(1498..4713)
                     /gene="carB"
                     /locus_tag="ACFNML_001373"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFC6392746.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAMNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTLEEIHEI
                     CAIDPWFLAQIEDLMKEEKAVSDGILSDLDFAALRRLKRKGFSDKRLAQLLNVSEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECEARPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEQLQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARCMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(4753..5364)
                     /locus_tag="ACFNML_001374"
     CDS             complement(4753..5364)
                     /locus_tag="ACFNML_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392747.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(6212..6601)
                     /locus_tag="ACFNML_001375"
     CDS             complement(6212..6601)
                     /locus_tag="ACFNML_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFC6392748.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSEAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(7174..7299)
                     /locus_tag="ACFNML_001376"
                     /pseudo
     CDS             complement(7174..7299)
                     /locus_tag="ACFNML_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687733.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(7345..8478)
                     /gene="carA"
                     /locus_tag="ACFNML_001377"
     CDS             complement(7345..8478)
                     /gene="carA"
                     /locus_tag="ACFNML_001377"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFC6392749.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATIEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAEDVLALNPDGVFLSNGPGDPEPCTYAIKAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         1992 a         2292 c         2393 g         2360 t
ORIGIN      
        1 atcaatcacg gtttcttgaa cattgtgtcc gagcgcggtc aaaacttctt tcgctttttt
       61 gtgaagcgtg cggtttaacc cgccgtgaga atgtccgaac gctttgccgc cgtctaataa
      121 taaaatattc atttgttacc tcgtttgtga attgatgaat gtattgtgcc gttttacttt
      181 ataatttaaa agtgcaaaaa taagaaaaca cttttgcgtc aaatgaaata atcagatgtt
      241 ccaacacacg ggatggcaca taaagcaccg ccctgtgtgc cgtcctgatt cggaaggggt
      301 tacacccctc ccaaataaag tctgatcctg ccgccctaaa gggcggggtt ccaaccgaaa
      361 aggaaacacg atgaaaacca attcagaaga actgaccgta tttgttcaag tggtggaaag
      421 cggcagcttc agccgtgcgg cggagcagtt ggcgatggca aattctgccg taagccgcat
      481 cgtcaaacgg ctggaggaaa agttgggtgt gaacctgctc aaccgcacca cgcggcaact
      541 cagtctgacg gaagaaggcg cgcaatattt ccgccgcgcg cagagaatcc tgcaagaaat
      601 ggcagcggcg gaaaccgaaa tgctggcagt gcacgaaata ccgcaaggcg tgttgcgcgt
      661 ggattccgcg atgccgatgg tgctgcatct gctggcgccg ctggcaacaa aattcaacga
      721 acgctatccg catatccgac tttcgctcgt ttcttccgaa ggctatatca atctgattga
      781 acgcaaagtc gatattgcct tacgggccgg agaattggac gattccgggc tgcgtgcacg
      841 ccatctgttt gacagccgct tccgcgtaat cgccagtcct gaatacctgg caaaacacgg
      901 cacgccgcaa tctacagaag agcttgccgg ccaccaatgt ttaggcttca ccgaacccgg
      961 ttctctaaat acatgggcgg ttttagatgc gcagggaaat ccctataaga tttcaccgca
     1021 ctttaccgcc agcagcggtg aaatcttacg ctcgttgtgc ctttcaggtt gcggtattgc
     1081 ttgcttatca gattttttgg ttgacaacga catcgctgaa ggaaagttaa ttcccctgct
     1141 cgccgaacaa acctccgata aaacacaccc ctttaatgct gtttattaca gcgataaagc
     1201 cgtcaatctc cgcttacgcg tatttttgga ttttttagtg gaggaactgg gaaacaatct
     1261 ctgtggataa ttctataaaa acctgccata aaattgtttt tactcactta ataactttga
     1321 gaaaaaataa ataagatgat gtatagccgc ggcaatggca gtctatatgg ggagcgtgat
     1381 agggctgttc cttatgtacg gcttgtgtgt ggcaacatcg gctttccaaa tgacaaaatg
     1441 ccgtctgaaa ccgtaaaaaa aaaggtttca gacggcattt tcaacctgat tcaggcatca
     1501 gttgcggttt ttcaaacgcc cgtgcagttc ttgaacgctg tacacgccca agtggtcgcg
     1561 gcttttcgcg ccttcgctca tcgcttcgcc gccggcggtg gtggtgtatt gcggcacgcg
     1621 ttgctgcaat gcgctttggc ggatgatgtg gctgtcggac acggattgcg gatcgctgga
     1681 aacggtattc acgaccagtg cgatttcacc gtttttcagc gcgtcgccga tgtgcgggcg
     1741 gccttcgggg actttattga tggtttggac aatcagcccg tgttcggtca ggtattgcgc
     1801 cgtgccgcgc gtggcgcaga tgccgtagcc taaggcttgg aagtttttgg cggttttaat
     1861 gacgcgttct ttgtcttctt cgcgcacgga gaggaagatt ttgccggtcg ggttgaggcg
     1921 ttcgcctgcg ccgagttggg ctttgtagta ggcttcgccg aagcttgcgc ccacgcccat
     1981 gacttcgccg gtggagcgca tttccggacc caaaatcgta tccacgcccg ggaatttgat
     2041 gaaggggaac acggcttctt taacggcata gaaatcaggg acgacttctt tttccacgcc
     2101 ttgttctttc agggaaatgc ctgccataca gcgtgcgccg actttggcga gcggcacgcc
     2161 ggtggctttg gagacgaagg ggacggtgcg gctggcgcgc gggttcactt ccaatacgaa
     2221 cactacgccg tcctgcacgg caaactgcac gttcatcagt ccgaccacgc ccagcgcgta
     2281 cgccatcgct ttggtttggc ggcggatttc gtcttggatt tcttcgctta aggagtaggg
     2341 cggcagcgag cagccggagt cgccggagtg gatgcccgcc tgttcgacgt gctgcatgat
     2401 gccgccgata accacatctt tgccgtctga aacgcagtcc acatcgactt caatcgcgtt
     2461 gttcaggaag aagtcgagca acacggggct gtcttcggaa acctgcacgg cttcgcgcat
     2521 atatttttgc aactgctcgg cagagtggac aatctgcatc gcgcgtccgc ccaaaacata
     2581 agacgggcgc acgaccagcg gataaccgat ttcttcggct ttgacgagtg cttcttcttc
     2641 gttgtgggcg atgcggttgg gcggctggcg caggcctaag tcgttcaaca ctttttggaa
     2701 gcgttcgcgg tcttcggcgg catcgatgct gtcggcggac gtgccgatga tgttcacgcc
     2761 gttttcaacc agcgcgttgg cgagtttgag cggggtttga ccgccgtaat gcacaatcac
     2821 gccccacggg ttttcggtgc ggacgatttc caacacgtct tccaatgtca gcggctcgaa
     2881 atagaggcgg tcgctggtgt cgaagtcggt ggacacggtt tctgggttgc agttgaccat
     2941 aatcgtttca aaaccggatt cgcgcagggc gagcgcggcg tgaacgcagc agtagtcaaa
     3001 ctcgatgccc tgaccgatgc ggttcgggcc gccgccgaga atcatcactt ttttgcggtc
     3061 ggaaggacgc gcttcgcatt cctcttcgta agtggaatag aggtaggcgg tttcggtggc
     3121 gaactcggcg gcacaggtgt cgacgcgttt gtaaaccgga tgcagcttca gcgcgtagcg
     3181 gtgttcgcga acttcttttt cgcttacgtt caacaattgt gccaaacgtt tgtcggagaa
     3241 gcctttgcgt ttcagacggc gtagggcggc gaaatccaaa tcactcaaaa tgccgtctga
     3301 aaccgctttt tcttccttca tcaagtcttc gatttgcgcc aggaaccaag ggtcgatggc
     3361 gcagatttcg tggatttctt ccagcgtgaa gccggcgcgg aacgcgtctg ccacaaacag
     3421 catacgctca gggccggggt tcgccagttc gcggcggatt tccgctttgt cggagcttct
     3481 cggattgaag ccgcacaagc cggtttccaa gccgcgcaag gctttttgga agctttcctg
     3541 aatcgtgcgg cccatcgcca tcacttcacc caccgatttc atctgtgtgg tcaggcggtc
     3601 gtctgcggca gggaattttt caaacgcgaa acgcgggatt ttggttacga catagtcgat
     3661 ggacggctcg aacgacgcgg gggttttgcc gccggtgatg tcgttgcgca actcgtccag
     3721 cgtaaagccg accgccagct tggccgccac cttcgcaatc gggaagcccg tcgctttgga
     3781 agccagcgcg gacgaacggc tcacgcgcgg gttcatctca atcacaatca tctcgccgtt
     3841 ttcagggttc atcgcaaact gcacgtttga gccgcccgtg tccacgccga tttcgcgcaa
     3901 taccgccaac gaagcgttgc gcatgatttg gtattccttg tccgtcagcg tttgcgccgg
     3961 cgcgaccgta atcgagtcgc ccgtatgcac gcccatcggg tcgaagtttt caatcgagca
     4021 gatgatgatg cagttgtcgt tcttgtcgcg cactacttcc atctcgtact ctttccagcc
     4081 gagaacggac tgctcaatca gcagctcgtg cgtgggcgag gcatcgaaac cgcgttcgca
     4141 aatcgccaaa aactcgtctt tattgtaggc aatgccgccg cccgaaccgc ccatcgtgaa
     4201 agaaggacga atcagggtag ggaagccgac ctgttcctgc gccgccaaag cttcgttcat
     4261 tgtgtggcag acaaaagatt tcgggcaaga caaaccgatt ttttccatcg cctctttaaa
     4321 gcggccgcgg tcttctgctt tgtcgatggc gtcttccgtc gcgccgatta attcaacatt
     4381 gtatttcgcc agcacgccgt tgcgcgccaa atccagcgca cagttcagcg cagtttgacc
     4441 gcccatggta ggcagaatcg catcgggccg ctctttggca ataatttttt ccaccgtctg
     4501 ccacataatc ggctcgatgt aggtaacatc cgccatttcg gggtcggtca taatcgtggc
     4561 ggggttggaa ttcaccaaaa tgactttata gccttcttca cgcaaggctt tgcaggcctg
     4621 tgcgcccgaa tagtcaaatt cgcaggcctg accgataacg atagggccgg cgccgatgat
     4681 aaggatggat tttaggtcgg tacgtttggg catgatgggt ctttctatga aatcaaatgt
     4741 ttagctaaaa atttattgct gattttgatt ggcttttaat tgctctacga cagtattggc
     4801 aatctctttt cctgatgggg ctgtaattgt gtaataaaag ctaatactgc ctatgaagaa
     4861 actgataatg gctatgatat aggctatata actttgtgtt ttcatttttt tgtgatcatg
     4921 tttgatctga tttaattctt ttaattcaga aataattgga gttaataaat gtttttgact
     4981 ttcagatcca tgcgtttcca aaagttcatt tattcctatt agtgttggtt ttagttcgtc
     5041 tgagcagcca tcaaaaggtt cttcttgttg gatttccttt tgtaactgtc taactaaatt
     5101 taaatttaat tcatcagtgt aatatagttg cttaataatt tttctggcag caaaattatt
     5161 aaggtttact gcttctgtca tttgttttat atcattaaga tctaggttat tgtgattatt
     5221 ttttaatata tcaattattc tagatttaaa tttctttttc tttatattct ctaaatatag
     5281 ctgaattgga ataaaaacaa ttataaagag caaaagaaat atatatgtta gtgccatagc
     5341 ccatgtggca attatattta tcatttatca atatcttcca tttggtttat atattgtctc
     5401 ataataagca agccgtagct cgcatgcagg gtgtgtgcgg tacgcacgcg ttctccattt
     5461 tccctgcaac cccaacccac cgtgtgcgtg ccttgcggca cacaccctac cgatgggttc
     5521 aaaggtcgtc tgaaaaaacg ctcgcggcgt ttgttccatc tgccttgcga taaaggctgc
     5581 ctgaaaaatt cccgctgttg atatttcggg cattctttct caaaccgcct gcaaccgcaa
     5641 aatccgctgc aacctgcccc ctcccccgtg ggggagggtt ggggagaggg cattccccga
     5701 atttcggcaa cctttcccaa caactcaacc accgaaatac aagccttgtg gcttgttgcc
     5761 ctctctctag ctctctccca cgggagagag gacggagtgg ctgtagggct aagggttctg
     5821 caaggaaaca ggttgtgcag gttgcttttt gcttttcaga cgacctgaaa tcaacggtaa
     5881 atccaagtcg cttttcaaac cgcctgcaac cgcaaaatct gctgaaacct actccctccc
     5941 ccgtggggga gggctgggga gagggcattc tccgaatttc ggcaaccttt cctaacactc
     6001 taaccgccaa gacacaagcc ttgcggcttg ttgccctctc tctagctctc tcccacggga
     6061 gagaggatgg ggtggctgtt ggggctaagg tttctgtaaa ctaatcaggt tatgcaggct
     6121 gctttttgtt tttcagacgg cctttataaa tgctcaattc agtcggcttt atcctattat
     6181 tttcaataat ccaatcaaaa tcagccatct gctatcgtgc agcttgcttt tccaattctt
     6241 gcaacacgag caggatttct gtcagcacat cgtttgtctg ctgtaaaatc tcgtgattcc
     6301 aaaaccgcag tacggtaaag cccaagctgt tgagatatgc cgtccgcgcg tggtcgtatg
     6361 cggcttgttc tgtgtgctgc cctccgtctg cttcgacaat caacttgggc gttacgcaca
     6421 taaaatcaac aatatagttt cccatcggct gctggcggcg gaatttatag ccgttcagac
     6481 ggcctgcccg caggtgctgc cacaattttg cttccgcctc gctcatttct tggcgcatgg
     6541 ctttggcgcg ttggcgcaag atggggttgt cggtggtcaa gagtttctcg tgcgggttca
     6601 ttttgttgtc gtgctgtttg ctgccctctc tctaactctc tcccacgggg agagaggacg
     6661 gggcggttgt tggggttaaa ggggctgtaa ggaaaacaag tcgttcaggc cactttttac
     6721 ttttcagacg acctgcaatc aacggttgta aatccaagtt gcttttcaaa ccgcctgtaa
     6781 ccgcaaaact tgctgcaacc taccccctcc cccgtggggg agggctgggg agagggcatt
     6841 ctccgaatcg cggcaatctt tccctaattc cctaactgcc tcaatacaag cctttcggct
     6901 tgttgccctc tctctaacgc tttcccacgg gagagaggac ggtgcggctg tagtgattaa
     6961 gggttttgca aggaaaacaa ggaaaacaag ttgtacaggc tgttttttat ttttcaagcg
     7021 acctgaaatc aacgcttgta aatccaagtc gcttttcaaa ctgcctgcaa ccgcaaaatc
     7081 tgctgcaacc taccccctcc cccgtggggg agggctgggg agagggcatt ttccaagttg
     7141 cggcaatctt tcccaaatcc cttagtcttc taaaatacaa gccttacggt tgttgccaaa
     7201 tgaaacttct tccataccca aaacttgcgt gtaaaacgca atggttcggt caatgtcggc
     7261 aacggtcagt accagatggt cgagtgcgct aatcttcatt tcccgctttc tttttcaggc
     7321 ggcggcagtg ccgtctgaaa gccattatgc ccgttttgcc gctttcatat tgccaatgaa
     7381 tttgtcaaac aaatagccga catcttgcgg accggggctg gcttcggggt ggccttggaa
     7441 gcagaacaca ggtttgtcgg tcagctcgat gccttgcaaa gtgttgtcaa acaaggattt
     7501 gtgggtaatg cgtgcgttag cgggcagggt gtcggcatca acggcaaaac cgtggttttg
     7561 gctggtgatg acgactttgc cgctgtccaa atcttgcaca gggtggttcg caccgtggtg
     7621 gctgaagcgc attttcaggg ttttcgcgcc gatggcgagg ctgatgagct ggtgtcccaa
     7681 gcaaatgccg aaaatcggtt tgccgctttc catcagtttt tgtacggctt tgatggcgta
     7741 ggtgcaaggc tcggggtcgc cggggccgtt ggataggaat acgccgtcag ggttgagtgc
     7801 caacacgtct tccgcgctcg tttgggcggg gacgacggtc aggcggcagc cgcgcgaggc
     7861 gagcatacgc aggatgttgg ttttcacgcc gaaatcgtag gcgacgacgt ggtaaggctg
     7921 ttcgtcaggg gtaacgaaac ccttgcccaa tgcccattcg ccttccgtcc attcgtaagt
     7981 ttccgtgcag gaaacttctt ttgccaagtc tttgccgacc atactgccga acgcggcgat
     8041 gagttcttgc gctttttcga ttgtggcatc cgcaccggtc agaatcgcgc cgccttgcgc
     8101 gcctttttcg cgcaacagcg tggtcaggcg gcgggtatcg atgtcggcga tggcgacggt
     8161 tttgttgcgt accagatagt cgtgcaggct ttcggaggcg cggaagttgc tgtgcaagag
     8221 cggcaggtcg cggataatca ggccggcggc ataaacgctg cggctttctt catcttcggc
     8281 gttggtgccg gtgttgccga tgtgtgggta ggtgagggta acgatttgtt tgcagtagga
     8341 cgggtcggtc aggatttcct gatagccagt catcgaagta ttgaacacga cttcgccgga
     8401 agtcgaacct tcgtaaccga ttgatgtgcc gtggaatacg ctgccgtcag cgaggacgag
     8461 gagggcgggg gtgctcatga tgggaatcct gtttttaata aaattcttga caatgccgtc
     8521 tgaagggact tcggcagggg gcatccggtc ggaaaaatcc ggtcaaaaaa aaacacgccg
     8581 cgcaagatag acgcgtaacg tgcttttcgg gcaagtgcct ataccctgaa agcagcatat
     8641 tttaagacga aacatacccg atttcaagcg caaacgcggc aggcgggctg atatgttcgc
     8701 gcgaaaacgg aaatccgtgg cgtaaaaccg cccgctccgc gcccactatg cccgttacgc
     8761 atttccccac gtccgtccgg cgaaactatg ggaatacccg aatcgtcagg aatacctgaa
     8821 tcgtcaggaa tacccgaacc gtcattcccg cgcaggcggg aatccagacc tgtcggcacg
     8881 gaaacttatc gggtacaacg gtttcttgag attttacgtc ctagattccc gcctgcgcgg
     8941 gaatgacgcg tttcatttgc cgcccctccc gaaaaacgca aaaaatgccg tccgaaaacc
     9001 tttcggacgg cattttcgcg tgcaaatcag tagaaga
//
LOCUS       DAXHTR010000086         9036 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.086, whole genome shotgun sequence.
ACCESSION   DAXHTR010000086 DAXHTR010000000
VERSION     DAXHTR010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.086"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            173..952
                     /gene="pgeF"
                     /locus_tag="ACFNML_001378"
     CDS             173..952
                     /gene="pgeF"
                     /locus_tag="ACFNML_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HFC6392750.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGTHVGDDPEAVRRNREIVQQQVGLPVAYLNQIHSTIVVNAAEALGGTPDADAS
                     VDDTGKAACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEM
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            1004..1483
                     /gene="lptE"
                     /locus_tag="ACFNML_001379"
     CDS             1004..1483
                     /gene="lptE"
                     /locus_tag="ACFNML_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFC6392751.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            1485..2483
                     /gene="holA"
                     /locus_tag="ACFNML_001380"
     CDS             1485..2483
                     /gene="holA"
                     /locus_tag="ACFNML_001380"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFC6392752.1"
                     /translation="MAAHIGHIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREVYTADNAFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLALLYPKGHTVNIDEAQTAVANVARFDVFQLAGA
                     WMKGDVPRVCRLLDGLQEEGEEPVLLLWAVAEDVRTLIRLAAALKQGQSIQSVRNGLR
                     LWGDKQTLAPLAVKRISVVRLLDALKTCAQIDRIIKGAEEGDAWTVFKRLVVSLAE"
     gene            2604..3023
                     /locus_tag="ACFNML_001381"
     CDS             2604..3023
                     /locus_tag="ACFNML_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392753.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            3099..3614
                     /locus_tag="ACFNML_001382"
     CDS             3099..3614
                     /locus_tag="ACFNML_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HFC6392754.1"
                     /translation="MMTLKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLYLLRLRF
                     IWACIFIVFIPMLAYAGLRHLLGISPKDAFGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(3698..4582)
                     /locus_tag="ACFNML_001383"
     CDS             complement(3698..4582)
                     /locus_tag="ACFNML_001383"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFC6392755.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDIRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDTENSRQSLELNSDLVNQLAEINKNLR
                     KHHDLAKLGVADILRFPGVLASQRENTEELAKSITELTEKALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLVEAVGDINNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIDEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIAAECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5066..5929)
                     /gene="rpoH"
                     /locus_tag="ACFNML_001384"
     CDS             complement(5066..5929)
                     /gene="rpoH"
                     /locus_tag="ACFNML_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFC6392756.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            complement(6193..7731)
                     /gene="lnt"
                     /locus_tag="ACFNML_001385"
     CDS             complement(6193..7731)
                     /gene="lnt"
                     /locus_tag="ACFNML_001385"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFC6392757.1"
                     /translation="MFSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFSLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTARSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQVGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            complement(7800..8526)
                     /locus_tag="ACFNML_001386"
                     /pseudo
     CDS             complement(7800..8526)
                     /locus_tag="ACFNML_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688345.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            8722..>9036
                     /locus_tag="ACFNML_001387"
     CDS             8722..>9036
                     /locus_tag="ACFNML_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HFC6392758.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLPARLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYSLREKAYSRIEMQSDTVLQAGIWRGTSCPT
                     ARS"
BASE COUNT         1993 a         2244 c         2637 g         2162 t
ORIGIN      
        1 taagggcatc attcccgcac ctgcaagaat ccgaatccga acgtttgaac aattgaaaac
       61 aatcccgaat ctccgaattc ccgcctgtgc gggaatgacg aaaaaacaaa catccatttg
      121 ccccgaaggc agttaatcaa ccctttccgc cacacaccta ttccaatatc caatgaaaac
      181 catcacagaa accctaaatc tcgccccgaa aggcaaaaac ttcctgaccg ccgattggcc
      241 cgcgcccgcc aatgtgaaaa ccctgattac cacgcgcaac ggcggcgtga gccaaggtgc
      301 gtatcagagt ttgaacctcg gtacgcacgt cggcgacgac cctgaagccg tgcgccgtaa
      361 ccgcgaaatc gtgcaacagc aggtcggatt gcccgttgcc tacctcaatc aaatccacag
      421 caccatcgtc gtcaacgctg ccgaagcgtt aggcggcacg cccgatgccg acgcttccgt
      481 ggacgacacg ggcaaggccg cctgtgccgt gatgaccgca gactgtctgc ccgttctatt
      541 ttgcgacagg gcgggtacgg cggttgccgc cgcacacgcg ggctggcgcg gtttggcggg
      601 cggcgtattg caaaacacca ttgctgcgat gaaggttccg cctgtcgaaa tgatggcgta
      661 tctcggcccc gccatcagtg cggatgcgtt tgaagtcgga caggatgtgt ttgatgcgtt
      721 ctgcacgccc atgcccgaag ccgccgccgc atttgaaggc ataggcggcg gcaaattcct
      781 tgccgacctt tacgcgctcg cccgcctgat tctgaagcgt gaaggcgtgg gcggcgtata
      841 tggcggcacg cattgtacgg ttttggaacg ggacactttc ttttcctacc gccgcgacgg
      901 agcgacaggg cgtatggcga gcctgatttg gctggacggc aatgccgtct gaacacgccg
      961 ctgatataat ctaccgactt tgtgcttttg agaaaggcaa gccatgaaca aactgtttct
     1021 tactgccgca gtgctgatgc tgggcgcgtg cggtttccac ctgaaaggtg cagacggcat
     1081 ttctccgccg ctgacctacc ggagctggca catcgaaggc ggacaggcat tgcagtttcc
     1141 tttggaaacc gcgctgtatc aggcttcggg cagggtggac gatgctgccg gcgcgcagat
     1201 gaccctgcgt atagacagcg tttcccaaaa caaggaaacc tacaccgtta cccgtgcggc
     1261 agttatcaac gaatatcttt taatcttgac ggttgaagcg caggtattga aacgcggcga
     1321 gccggtcggc aaaccgatga ccgtgtccgt ccgccgcgtc cttgcttatg ccgacaacga
     1381 gatcttgggc aaacaggaag aggaagcggc attgtgggcg gaaatgcggc aggatgccgc
     1441 cgaacagatt gtccgccgcc tgacctttct gaaggcggaa tgacgtggcg gcacatatcg
     1501 gacacattga tacggacgcg cctttgaaac ccctgtacgt catccacggc gaggaagaac
     1561 tgttgcgtat cgaggcattg gacgcattga gggcggcggc gaagaaacaa ggttacctta
     1621 atcgggaagt ttatacggca gacaatgcct tcgattggaa cgaactgttg caaaccgcag
     1681 gcagtgcggg tctgtttgcc gatttgaagc tgttggaact gcatatcccc aacggaaaac
     1741 ccggcaaaac cggcggcgag gcgttgcagg attttgccgc ccgcctgccg gaagatacgg
     1801 taacgctggt tttgctgccc aaactggaga aaacccagct ccagtccaaa tggtttgccg
     1861 cattggcggc aaagggggaa gtgtgggaag ccaaaccggt cggcgcggcg gccttgcccc
     1921 aatggatacg cggacggctg gacaaaatcg gtttgggtat cgaggcagac gcattggcac
     1981 tgtttgccga gcgcgtggaa ggcaatttgt tggcggcgcg tcaggaaatc gacaagctcg
     2041 cgctgctgta tccgaaaggg cataccgtca atatcgatga ggcgcaaacc gccgttgcca
     2101 atgttgcccg ctttgacgtt tttcagcttg ccggcgcatg gatgaagggc gatgtcccgc
     2161 gcgtatgcag gcttttggac ggattgcagg aggaaggcga agaaccggtg ctgcttctgt
     2221 gggcggttgc cgaagacgtg cggacgctga tccggcttgc cgccgccttg aagcaggggc
     2281 agagcatcca atccgtccgc aacggcctca ggctttgggg cgacaagcag acgctcgcac
     2341 cgcttgcagt caagcggatt tccgtcgtcc gcctgcttga cgcgctcaaa acctgtgccc
     2401 aaatcgaccg aatcatcaaa ggcgcggaag agggcgacgc atggacggta ttcaaacggc
     2461 ttgtcgtgtc gctggcggaa taaagcggta atccccaaaa tccgaaaata ctgtaaaata
     2521 ccgttaatcc tgaaaagtat tcaccaatcc gtccgaaaac atttcagacg gcacgaccac
     2581 ctcaataaag gaacattaac cctatggaca ataagaccaa actgcgcttg ggcggcctga
     2641 ttttactgac caccgccgtt ttaagcctca ttatcgtatt gattgtcgac tcctggccgc
     2701 ttgccatcct gcttgccgcc gtcatcgtcg ccgccgctgc gggcggtttt gtttggacat
     2761 cccgccgaca gcaacgccag tttatcgaac gcctgaaaaa attcgacatc gatcccgaaa
     2821 aaggcagaat caacgaggca aacctgcgcc gtatgtacca cagcggcgga caacaccaga
     2881 aagatgcgat taccctgatc tgcctgtcgc aaaaatgttc ggtggacgag gcgcacgcta
     2941 tgttcaaaaa acgcccgaca cgtcaggaaa tcaatcaaat ggcggcaaaa cagtcgcgcg
     3001 gtcagaaacg tccgcaccgt taaccgccgc aaggcatctt tgcataaatg ccgtctgaag
     3061 cctgttggcg tttcagacgg catattctga ttgaaaagat gatgacactg aaaaccgccc
     3121 cgctcaaacg ccgctttgcc gccatgctgt acgaaatgct gctggtcggt gcggcaacct
     3181 gtttggcagc attgtttgcc ggtattgccg ccatttttct gaatcccgtt tctatcgcgg
     3241 tttctgcatt ggtaacaagt atcctgataa tgggatcatg gtggctttat tttcgcgcca
     3301 actggcacgg tcaggggcag accttggcga tgaggacatg gaaaatcggc ttgtgcgacc
     3361 ttaacggcat acagccgtct ttgtacctgc tgcgcctgcg ctttatttgg gcgtgcatat
     3421 tcatcgtctt cattcctatg ttagcctatg ccggattacg ccacctgctc ggtatttcac
     3481 ccaaggatgc attcggtgcg gcattgattt ggctgatttt accgtggggg tttgccctgc
     3541 tgaatcccga tcggcagttt ctgtatgatt ttcttgcagg aacaagattg gtggcggtca
     3601 aaggaaagcc ttaagccttt ataccgcaaa ggtttcaacc tgaaaaaatg ccgtctgaaa
     3661 gggctttcag acggcatttg gttatcgggg aaaccgatta ttcgatattc tgcacttgtt
     3721 cccgcatctg ctcgattaag actttcagtt cgaccgaggc ttgggtgcat tcggcggcaa
     3781 tggatttgct gcccaaagtg ttggcttcgc ggtttaattc ctgcatcagg aaatccaagc
     3841 gttttccgct gctgcctttg tgttcggtaa cgatgcggcg tacttcgtcg atatgcgtgc
     3901 gcaggcggct gaattcttcg tcgatgtcgg atttttgaat aaagagggca aattcctgtt
     3961 gcagcctgtc attattgatg tcgccgactg cttcgacgag gcgggcgcgg attttttctt
     4021 tatgtgtttc cagcagggta ggaaagagtt cgctcaatgc atctatgatt tcttccatgc
     4081 tttcgaggcg ttgtaacagg tgctcgccca attttttccc ttcgcgccgg cgtgcggcag
     4141 taaagtcttt taacgctttt tcggtcagtt cggtaatgct ttttgccaat tcctccgtat
     4201 tttccctttg gcttgccaat acgccgggga aacgcaggat gtcggcaacg cccagttttg
     4261 ccaaatcgtg atgcttgcgg aggtttttgt tgatttcggc aagctggttc accaaatccg
     4321 agttgagttc caaggactgt ctgctgtttt ccgtatcttg aatttggatt ttgcattcga
     4381 ctttgccgcg cgcgatatgg gatgaaattt tctcgcggat accgctttcc aaatagcgca
     4441 aatcatccgg catcctgatt tgaatgtcca aaaagcggtg gttgacggcg cggatgtcga
     4501 gattgatgcg tttgctgccg cactctgccg ccgcgttggc aaatccggtc atgctgtgga
     4561 tgtggatatt tccgctgctc atgtcgttct ccgaagcccg ttaaaatgga atcaatatat
     4621 cacatctgta tggcggcaag cgttttcggg tgtgaaaaat tgaagatttt gcagcggcag
     4681 attggaatca cgcgcttttg ttgctgcaag gaagggaaat gtatagtgga ttaaccaaaa
     4741 ccagtacggc gttgcctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
     4801 caagcgaatc ggttccgtac gatttgtact gtctgcggct tcgccgcctt gtcctgattt
     4861 ttgttaatcc actatatcaa tttcgccaat ctgtcggaaa agcagctgat gcggcagtgt
     4921 ctggtgcatg tctgcttttt gatttcggcg attgcgatgg cttggacgga taaaatcgtg
     4981 tacggcacga cgcacaaacc gcattgatgt ttaccaaata aaatacccga caaaacaatt
     5041 tgtcgggtat tttattgcgt atatttcaaa ccgcttcggc ttcttcggtc aggaaaccac
     5101 gcagtttctg catggctttc gcttcaatct ggcggatgcg ctcggcagag acgccgtatt
     5161 ctgcagcaag ctggtgcagc gtcagaccgc cgtcatcttg aagccagcgg ctttccacaa
     5221 tacggcggct cctgtcatcc agttgcgcca aagcgttttg taaaccttct gtttgcaggg
     5281 cgtaatgcgc ctgtttcgat agttgtcggc tcggttcgga atcgtggtcg gcaagccagt
     5341 cgatgggggc gaaactgtcc tcgtcgtcgc tgttgtctgc catgatggcg atgtcgtgtc
     5401 ccgtcatgcg ctgttccatt tccagaactt cggaaagttt gacacccaaa tcgtcggcga
     5461 tgtcttgtgc ctctttggga gacagggcgt tgaggttttt acgcatactg cgtaaattga
     5521 aaaacagctt gcgttgcggt ttggtggtgg caacgcgaac caaacgccag tttctcaaaa
     5581 taaactcgtg gatttcggct ttaatccagt gtacggcaaa tgaaaacaga cgcgcgcctc
     5641 gacctggctc gtagcgtttg accgccttca tcagtccgat attgccttcc tgaatcaggt
     5701 ctgcctgatt cagcccgtag ccgtcatagc cgcgcgcgat ggaaacgacg acgcgcaggt
     5761 gggacaggat gagttgtttg gcggcgttga ggtcgccctt tatccggcgt tcggcgaggc
     5821 gggtttcttc ttcttgggac agcatcggga tgctgttgac ggtgtggatg tattgttcga
     5881 ggctgccgtt gccgctttgg atggcgggta atgcgaaagc gttattcatt tggggcattt
     5941 cctttcggct gaaactgcgt atcggcggtt tgctgtgttg ggatgcagta tatcactgct
     6001 tggcttgtat tttgtatgtt tggcaggaga tatgcgctaa ggtttgaaag acaggaaaat
     6061 tttgtaaggc aggtttgatt gattttgtaa acctgatggc tcaattcgat ttcagaatta
     6121 tattacatac gtaattgtat gtaaatagcc gttttgaaaa aagacagccc gtccggacgg
     6181 gctgtgcagg tatcagtgtt ctttgtttcg gaagatgaaa agaatcagtg cggctagggt
     6241 caatatgccc atcaaccacc atgaactgcc ggttttcata tagggcgttt cgccgacata
     6301 gcctttgatg tgtccttcca atacggtttc cgtatcgggt tgggcttggg cgatgatgtt
     6361 gcctttgggg gagatgatgg cggttgcgcc ggtgttggtg gcacggacca tatagcgtcc
     6421 gagttccata gcccgcgcct gcgattgttg gaggtgctgg tacatggcgt tggattttcc
     6481 gtaccacgcc atattgctgg cattggcaag cagggtggca tctttggcgg cggcaatcag
     6541 ttcgtcgccg aatccgtctt cgtaacagat gttgaaggcg attttttggt ttttcatcag
     6601 cagggcggat tgcttaccgc cgcctttgcg gaagtcggaa aggggcatat tcatcatttt
     6661 gtaaagcggc gtggtcagga agggcagcgg tttgtattcg ccgaagggga cgaggtggtt
     6721 tttggcgtag taggggatgc cgtcctgatt gttttcctga taaccggtca ggttgatgac
     6781 ggcgttttcg taaccgttgc cgtccgaagt gtattggctg atgccgacgg cgagcgcgct
     6841 gccgttgttt tgcgcctgtt cggcaaattt tgccagtatg ttttccggca ggttttggcg
     6901 cataacgggg atggcggttt cgggcaggat gacgatgtcg gcagtggttt tgccgacctg
     6961 ttcgtaatat ttctgtatgg tcgggatgac ttggtcttca cgccatttga gggtttggtc
     7021 gatgttgcct tgaagcaggg cgacggtgct gcggctgccg tcggggcggg tgaagtcggt
     7081 ttgtcgggcg gtgtagcctg cggcaagcag ggcggcaatc aggataattg gaagcaggcg
     7141 tttgcccgaa cgtgcggtgt tatcactcgc caaaaccagc cagacaccga gaaaggcggt
     7201 tgccagtgta accatgtgga tgccgcccaa tggggcaaag ccggcgagcg ggctgtccgg
     7261 ggtgatttgg gagtagccga ttgcgcccca gccgaatccg gtcaggaaac gttcgcgggc
     7321 aaactcggtc agtgtccaca ggatgggcag taccaaaccg atttttatgc cccgaggcag
     7381 ggtaaatttt ttccacagcc agaaacacag tgccggataa agggcaaggt aggcggggag
     7441 taggaaagtc agcggtacgg catagaggtc gggcaggccg gaaacgtcgt gcagggcggt
     7501 gtgtatccag tagaactgtg tcgtgtatgc ggtcaggctg aacaggtagg cggaagagac
     7561 agcaaaacgc ggacgcagtt cgatgaggcg gacgaaggca ccgaaaatca agggcatcag
     7621 ccaaaagtgg tagtagggtg cgaaggtaaa gggggtggcg gcggcaaaaa ggatgagcaa
     7681 aggccagtag agggcggggt gctgccagta tttgtccagt ttggaaaaca tattcatctg
     7741 tctgttcgga agataccgtc tgaacatctt tcaaacggca tcggtatttg aaaaagaaat
     7801 caatgcctgc cgaaacgatt catcagcggc aaggcggggg cgcaggcaat cgaaccggaa
     7861 agcagcccga caacgaggtt ggcgaagtac tccgcccagc cagcgtccca gtgttgcgcg
     7921 tgcaggaaat cgtgcagaag gccgaggttg tgggcgacca gtacgccgcc gacaagaaac
     7981 atggcaagcg tgccgaccac gctcaaaccg cgcataaagc aaggcataaa ggcagtcagc
     8041 atttgcccca aactgcgcga aaaggtttgt gggcggcgca tcagcagcat gcctaagttg
     8101 tcgagtttga cgatgacggc aacgattccg tacaccaaaa cagtcatgcc gatgccgatt
     8161 gccgccatta cgagcatgcg cgtcatgtcg gtgcagaaac ttgtgcagca gcttttctac
     8221 gccgtcaaag cacagataaa tgccgcctgc cgtcaaaagc ggcgtaatga gttgcggcag
     8281 gaaggcggaa agcagcaggg ccgcaggcac caaaaccggc ttgttggaaa aagaaccttt
     8341 cgccatcgac caaacaatcg gcaactcgcg ttctgccgat acgcccgtaa cccggttggc
     8401 attgggtgcc aaatcgtcgc cgaccacgcc ggcggttttc tttgcggcgg ctttggtcat
     8461 cagggcaaca tcgtccaaaa cggcggtgat gtcgtccggc agggtaaata gtgaggcaaa
     8521 tgccattaaa gaatcctgaa atgcggcgca aagtccgaca ttatatagga gaacgcggat
     8581 ttgggcggtt tcaggcggca tgaaacagga aaatgccgtc tgaacgctgt ggcggacgtg
     8641 aagtaaagtt tcgtgaaaag aaaatgccgt gttacagtct ttcgatttta atttcatgaa
     8701 ttttaaggga gaatcgttag cgtggattgg atgggcagtc tgttcctgcc gggtggcgca
     8761 ctgttgtttc tgagcgtggt ttcgaccact ttacccgcac gtttgggaat gcctttgctg
     8821 ctggtttctc ccgccggcgt gttggacggg gcggcggaag ccttggcgat tgcggcgttc
     8881 ctgatgctgg tcgcgcgtcc gccggcagtg ttcggcggtt tgtggaaatt caattacagc
     8941 ctgcgtgaaa aggcgtatag ccgaatcgaa atgcagtccg acaccgtgct tcaggcgggg
     9001 atttggcgtg gtacatcctg cccgacggca aggtcg
//
LOCUS       DAXHTR010000087         9031 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.087, whole genome shotgun sequence.
ACCESSION   DAXHTR010000087 DAXHTR010000000
VERSION     DAXHTR010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9031
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.087"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            33..1925
                     /gene="mafB"
                     /locus_tag="ACFNML_001388"
     CDS             33..1925
                     /gene="mafB"
                     /locus_tag="ACFNML_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HFC6392759.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGNIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGAMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISESSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPIKGIGAVRGKYGLGGI
                     TAHPVKRSQMGEIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLANKRHPKTKVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQSSQFKAHAQREWENKTGLDFNHF
                     IGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATVEIKKPDGSWEMKKGRGG
                     KPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQKWRGTSKSGVEIEGFTEPNRTAY
                     PLYNSK"
     gene            1952..2371
                     /gene="mafI"
                     /locus_tag="ACFNML_001389"
     CDS             1952..2371
                     /gene="mafI"
                     /locus_tag="ACFNML_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MafI"
                     /protein_id="HFC6392760.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            2461..2532
                     /locus_tag="ACFNML_001390"
                     /pseudo
     CDS             2461..2532
                     /locus_tag="ACFNML_001390"
                     /inference="COORDINATES: protein motif:HMM:NF026862.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 17 domain-containing
                     protein"
     gene            2529..2771
                     /gene="imm31"
                     /locus_tag="ACFNML_001391"
     CDS             2529..2771
                     /gene="imm31"
                     /locus_tag="ACFNML_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm31 family immunity protein"
                     /protein_id="HFC6392761.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            2849..3094
                     /locus_tag="ACFNML_001392"
     CDS             2849..3094
                     /locus_tag="ACFNML_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFC6392762.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PNYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            3097..3486
                     /locus_tag="ACFNML_001393"
     CDS             3097..3486
                     /locus_tag="ACFNML_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392763.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDGGIADIDNIVGFVSSCSLSKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALEKYPEIYQKFQHFFPFVELPPL"
     gene            3534..3704
                     /locus_tag="ACFNML_001394"
                     /pseudo
     CDS             3534..3704
                     /locus_tag="ACFNML_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3707..4105
                     /locus_tag="ACFNML_001395"
     CDS             3707..4105
                     /locus_tag="ACFNML_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392764.1"
                     /translation="MLGLTRNNCDDLGFAILCYERNAADKKEFLDWLYGIVRTVPAES
                     FPRYLIDLIDCLESDDCSINIYNIIGFVPTTGMTKKEKDAVYGITYIRNQEFYDIPIT
                     REKALKALKKHPEIYQKFRHFFPFVELPPL"
     gene            4206..4595
                     /locus_tag="ACFNML_001396"
     CDS             4206..4595
                     /locus_tag="ACFNML_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392765.1"
                     /translation="MWKIIKEDSNDLGFAIKCLFSQSIDLNEFKLWIEQVIRDMPIED
                     IPFYIFDLADFDVGIGDIGNIVGFAPSSSLPKSKKNALTGIAFLRGIDVYDPPVSKEK
                     ALKALKKHPEIYQKFQHFFPFVELPPL"
     gene            4743..5132
                     /locus_tag="ACFNML_001397"
     CDS             4743..5132
                     /locus_tag="ACFNML_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392766.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLF"
     gene            complement(5173..6630)
                     /locus_tag="ACFNML_001398"
     CDS             complement(5173..6630)
                     /locus_tag="ACFNML_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HFC6392767.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMGWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFANHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILAAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(6645..7475)
                     /locus_tag="ACFNML_001399"
     CDS             complement(6645..7475)
                     /locus_tag="ACFNML_001399"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HFC6392768.1"
                     /translation="MAHYAIGDIQGCFDELTSLLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRIAKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLEGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(7553..7942)
                     /locus_tag="ACFNML_001400"
     CDS             complement(7553..7942)
                     /locus_tag="ACFNML_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFC6392769.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAVAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(8078..8602)
                     /gene="nspA"
                     /locus_tag="ACFNML_001401"
     CDS             complement(8078..8602)
                     /gene="nspA"
                     /locus_tag="ACFNML_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HFC6392770.1"
                     /translation="MKKALAALIALALPAAALAEGASSFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSTGLGVLAGVSYAVTPNVDLDAGYRYNYIGKVNTVKN
                     VRSGELSAGMRVKF"
     gene            complement(8772..>9031)
                     /locus_tag="ACFNML_001402"
     CDS             complement(8772..>9031)
                     /locus_tag="ACFNML_001402"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392771.1"
                     /translation="QTNRRPPGKTRPERVRKICLSMENAAASPAGRFRRHSRRRKCPT
                     LKAQTHTKPYACTCNNPTDTAVFSKPFASFTHPPRDAAATI"
BASE COUNT         2450 a         1833 c         2486 g         2262 t
ORIGIN      
        1 tttcattccg taccatctta aggaacatca aattgggcat ttcccgcaaa atatccctta
       61 ttctgtccat actggcagtg tgcctgccga tgcatgcaca cgcctcagat ttggcaaacg
      121 attcttttat ccggcaggtt ctcgaccgtc agcatttcga acccgacggg aaataccacc
      181 tattcggcag caggggggaa cttgccgagc gcagcggtca tatcggattg ggaaacatac
      241 aaagccatca gttgggcaac ctgatgattc aacaggcggc cattaaagga aatatcggct
      301 acattgtccg cttttccgat cacgggcacg aagtccattc ccccttcgac aaccatgcct
      361 cacattccga ttctgatgaa gccggtagtc ccgttgacgg atttagcctt taccgcatcc
      421 attgggacgg atacgaacac catcccgctg acggctatga cgggccacag ggcggcggct
      481 atcccgctcc caaaggcgcg agggatatat acagctacga cataaaaggc gttgcccaaa
      541 atatccgcct caacctgacc gacaaccgca gcaccggaca acggcttgcc gaccgtttcc
      601 acaatgccgg cgctatgctg acgcaaggag taggcgacgg attcaaacgc gccacccgat
      661 acagccccga gctggacaga tcaggcaatg ccgccgaagc cttcaacggc actgcagata
      721 tcgtcaaaaa catcatcggc gcggcaggag aaattgtcgg cgcaggcgat gccgtgcagg
      781 gtataagcga aagctcaaac attgctgtta tgcacggctt gggtctgctt tccaccgaaa
      841 acaagatggc gcgcatcaac gatttggcag atatggcgca actcaaagac tatgccgcag
      901 cagccatccg cgattgggca gtccaaaacc ccaatgccgc acaaggcata gaagccgtca
      961 gcaatatctt tacggcagtc atccccatca aagggattgg agctgttcgg ggaaaatacg
     1021 gcttgggcgg catcacggca catcctgtca agcggtcgca gatgggcgag atcgcattgc
     1081 cgaaagggaa atccgccgtc agcgacaatt ttgccgatgc ggcatacgcc aaatacccgt
     1141 ccccttacca ttcccgaaat atccgttcaa acttggagca gcgttacggc aaagaaaaca
     1201 tcacctcctc aaccgtgccg ccgtcaaacg gaaagaatgt gaaactggca aacaaacgcc
     1261 acccgaagac caaagtgccg tttgacggta aagggtttcc gaattttgag aagcacgtga
     1321 aatatgatac gaagctcgat attcaagaat tatcgggggg cggtatacct aaggctaagc
     1381 ctgtgtttga tgcgaaaccg agatgggagg ttgataggaa gcttaataaa ttgacaactc
     1441 gtgagcaggt ggagaaaaat gttcaggaaa cgagaagaag gagtcagagt agtcagttta
     1501 aagcccatgc gcaacgagaa tgggaaaata aaacagggtt agattttaat cattttatag
     1561 gtggtgatat caataagaaa ggcacagtaa caggagggca tagtctaacc cgtggtgatg
     1621 tacgggtgat acaacaaacc tcggcacctg ataaacatgg ggtttatcaa gcgacagtgg
     1681 aaattaaaaa gcctgatgga agttgggaaa tgaaaaaagg aaggggcgga aaacctatga
     1741 caaatcattc tatgttccca aaagattggg atgaggctag aattagggct gaagttactt
     1801 cggcttggga gaaaagaaag gaattagaag gtcaaaaatg gagaggtaca agtaaatctg
     1861 gtgttgaaat agaaggattt acggaaccta atcgaacagc ttatccactt tataatagta
     1921 aatgaagtat tgattaaaaa ggtgttttta aatgtataaa ataaaatgtt tttatgatca
     1981 gtttagttct ggtgaattat ttaactactg ccagttttta gataattctt ctaatcaaaa
     2041 gataaataca cgaggaacgg tgtatcaata tattatttat gttttaacag gagaccttta
     2101 tctacaaaaa gacatagatg aaaatttaga atttatccat caagcagaaa ataatcccaa
     2161 tgaagtttat tcaggaggtg gacaaggttt ttgctgggat atttctgctg aaaaagtagt
     2221 tttctatcac aacgaatttg atgaagaaga cggctggcct gatttaagct gttcgctgca
     2281 tacttttaaa actgctttaa ttgcttggaa tgcttttttg caattgccta aaagtattca
     2341 ttcggtggtg gagactgtga ttgaggaata agcataatta gcttaatgaa tagaatcagc
     2401 gatatagatt ggactgcaaa tccacgctta tacgctgtgc catgattaac aggaagagag
     2461 caacttggat gggctagtag ggatggtaag catttaaata tatcaattga tggaaagatt
     2521 acacacaaat gactaattta aaattagatt tctactctga agttattata aaagattctt
     2581 gccctaatga tttgttagaa aatggtgaga ctattaaagg aaaaaaagga gtggtattag
     2641 gtataagcga agaagatggt ataatctatg gctatactat tttacttttt gatataaaat
     2701 attgtatata tatagataag aaatatatta taccgactgg aaaaaaattc tctcgcgatg
     2761 atttttatta gctaatttaa gtaatttaag caagtttttt agatactgaa actggtaatt
     2821 atgtttatgt aagcataagg agttcagatt gaaggattct acagggcaaa tgaggctggc
     2881 aaccaaggat ttggcggaag ccattaaacg aggagaagta cgtagttctg cttttacaac
     2941 aaagcaacta aaggcaatcg aaaaaggaga atctaaaata ccaaattaca cttggcatca
     3001 tcatcaagac actggaagga tgcaattgat taatgaagac ttgcatcatg ataccggcca
     3061 tatcggttgg agagcgatga gtaaaggaaa gtaactatgt ggaaaatcat aaaagaggat
     3121 agtaatgatt taggatttgc aattaaatgc ttattctctc agtctattga tttaaatgaa
     3181 ttcaaattat ggattgaaca agtaatacga gatatgccca tcgaggacat cccgttttat
     3241 atttttgatt tggcggattt tgatggggga attgccgata ttgacaatat tgtaggtttt
     3301 gtttcgagtt gcagcctatc aaaatcgaaa aaaaatgcct tgacgggcat tgccttctta
     3361 aggggtatag atgtctatga ccctcccgtt tcaaaagaaa aagcattaaa agccttagag
     3421 aaatatcctg aaatttatca gaagtttcag catttctttc cgtttgtaga gctgcctccg
     3481 ctttaaacag tcaaaatgcc gtctgaaaca atattcggct ttcagacggc atttttgata
     3541 caaagcaggt aaccaaaaga gtgtttgacg gaaaaggaga agctaaaata ccgagctata
     3601 cttggcatca tcatcaggat acgggacgaa tgcagttaat atccgaaaga aagcatacgc
     3661 aaacagggca tataggtggt gaagcaatgg ataaaggaaa atgattatgt tggggcttac
     3721 acggaacaat tgtgatgatt tgggatttgc tatattgtgt tatgagcgca atgcagccga
     3781 caaaaaagaa ttcttggatt ggttatatgg aattgtcagg actgtccctg ccgaaagttt
     3841 tccaaggtat ctgattgatt taatagattg tttggaatca gatgattgtt ccataaatat
     3901 atataatatt attggctttg ttccaactac cggtatgacg aaaaaggaaa aagatgccgt
     3961 atatggcatt acctatatac ggaatcaaga gttttacgat attccgatta ctagggagaa
     4021 ggcgttaaaa gccttaaaaa aacatcctga aatttatcag aagtttcggc atttctttcc
     4081 gtttgtagaa ctgcccccgc tttaaacagt caaaatgccg tctgaaacga tattcggctt
     4141 tcagacggta tttttgatat aaagcgggta actaaaagag cgtttgacgg caaaggaaga
     4201 taattatgtg gaaaattata aaagaggata gtaatgattt aggattcgca attaaatgct
     4261 tattctctca atctattgat ttaaatgaat tcaagttatg gattgaacaa gtaatacgag
     4321 atatgcccat tgaggacatc cctttttata tttttgattt ggcggatttc gatgtaggaa
     4381 ttggcgatat tggcaatatt gtaggttttg ctccgagttc cagcctgccg aaatcgaaaa
     4441 aaaatgcctt gaccggcatt gccttcttaa gggggataga tgtctatgac cctcccgttt
     4501 caaaagaaaa agcattaaaa gccttaaaga aacatcctga aatttatcag aaatttcagc
     4561 atttctttcc gtttgtagag ctgcctccgc tttaaacagt caaaatgccg tctgaaagac
     4621 cgtccgccgt tcagacggca tttttgatat aaagcggata actaaaagag cgtttgacgg
     4681 caaaggagaa tctaaaatac cggatgtatc ggttgggaag caatggataa aggtaaataa
     4741 ttatgtggaa aattagtaaa gaaaattgtg aagatttagg atttgcaata gtctgtatgt
     4801 tctatgatgc tattaatctt tctgaattta aattatggtt ggatatagtt gtcagagata
     4861 ctcctattga tacaattcca ttgtatattt ttgatttgat tgattttgat aagagtatag
     4921 gggaaattta tgatgtaatt ggagtcgtta attatggtta catttcaaat gatcaaaaaa
     4981 atgcattaac gggcattgcc ttcttaaggg ggatagatgt ctatgatccg cctatttcaa
     5041 aagaaaaagc attaaaagcc ttagagaaat atcctgaaat ttatcagagg tttcagcatt
     5101 tctttccttt tgtagagctt ccgctttttt aaaagacaat atgccgtctg aaaagttttc
     5161 agacggcatt ttttatttct tccagtaggc gggggtgaag aggatgaaga cggtgaagat
     5221 ttccagcctg cccaagagca tggcggtaac gcagatccat ttctgcatca cgtccaaacc
     5281 ggcgtaattg ccggcgggcc cgacttcgcc caggccgggg ccggcgttgg tgatgcaggc
     5341 gatgacggcg gtgaaggcgg tggtaaattc cataccgctc gccatcagca ggaagctgaa
     5401 gaggacgacg gtcataaagt agatgaagat gaaggacata acggtcagcg cgaggcggtc
     5461 gggtatggcc ttgccgctga ttttgacggt gcggacggct ttggggtgca gcagcaccat
     5521 catttcgcgc aggctgaatt tgaacaggac gagggcgcgt atggttttga tgccgccgcc
     5581 ggtcgagccg gagttggcga ggatgttggc gaggaaaaac atccacaggg aaatcaggag
     5641 cggccattgt gcgaagtcgg tgttggccag cccgtttgcc agtccgatgg agacgaagtt
     5701 gaaggcggtg tagcgcaggg attcggtaaa accggcgtaa tagccggtgt gccacaggta
     5761 cagggcggcg gcaaggatgc tgccggagag cagcagcagc atcgtccggc attcttcgtc
     5821 tttccaatag gttttgaggc tgcggctgtt gagggcggcg aaatggttgg caaaattgat
     5881 gccgccgaca atggtgaaaa cgatgatgac cgcttcgatg aggggggagt tgtaataagc
     5941 tatgctggca tcgtgggtgg aaaacccgcc cagcgagagg gtcgccatcg cgtgacagac
     6001 ggcatcgaac cagcccatcc cggcaaaatg caggcaggct gccgcgagga tggtgatcag
     6061 ggtgtagccg aaccagagtt ttttcgccac ttgggaaatg cgcggcgaca ttttgctttc
     6121 tttgtcaatg ccggggattt cggctttgaa taattgcgtg ccgcctacgc cgagcatagg
     6181 caggatggcg acggcaagga cgatgatgcc catcccgccc agccagttga gcatatgccg
     6241 ccaaaagttg acggaggggg cgagcccgtc gacgtggggg atgacggtcg cgccggtggt
     6301 ggtcagtccc gacatcgatt caaaaaatgc gtcggtaaag cccatattcg ggaaatacag
     6361 gtacatcggc atcgcagcca tagcggcaaa cgccagccac aacatcagga cgagggtgaa
     6421 gccgtcgcgc gggcgcagtt cgcgcctgaa ccggagggtg gcgagccgga cgatgtacga
     6481 gccggaaagg gtaacggtcg cggtggtggc gaaggcggtg tacgcgccgt ccgaaaaggc
     6541 gtaggagagg gcggcgggta tcagcaggat aaaggaaaac agcataccca gtcgggagag
     6601 gacgtgggcg atgggcagga ttttgtgcat agtggggcgg tccgttattt tgcgaagctt
     6661 ttccagtcta tgccgccggc ggcttggact tgggtaattt cttccgtttc gaggttgacg
     6721 gcggtcagct gtccgcccca cagcgcgccg gtgtccagcg agatgacgtt gtcggcattc
     6781 gtgtagccca gcgaggacca gtgtccgaag atgatggtgc ggtcgaggtt ttgccggtcg
     6841 ggggctttga accacgggcg caggtaaggc ggcatttttt tcactgtgga tttgtagtcg
     6901 aaatccagtt cgtttttaaa ggtcagggcg cgcatccgcg tgaaggcgtt gacgatgaag
     6961 cgcaggcggg catagccttc caaaccttcg tcccacgcgg ccggtttgtt gccgtacatt
     7021 ttggagaaga atttgacgta ttttttgccg cgcagttcgg cttcggcttc tctagcgagc
     7081 gattcggctt tggcgatgcg ccattgcggc aggatgccgg cgtgtaccat cacgcggctg
     7141 ccctcgcgta tcaacagcgg ttgcgcacgc agccagtcga gcattttttt tccgtcgggg
     7201 tgtttgagta tgggttcgat tgtgtcgctg cgtttgggcg cgccttcgcc gcagccgaca
     7261 gcgagcaggt gcaggtcgtg gttgccgagg acgatttgca cgctgttttc gtgccggatg
     7321 cagaattgca gcgtttcgag ggatttcggg ccgcggttga cgatatcacc cgtcagccag
     7381 agggtgtccg tgccgtggtt gaagccgatt ttgccgagca gtgaggtcag ttcgtcgaaa
     7441 cagccttgta tgtcgccgat tgcgtaatgt gccattgcag atgttgtgaa gtgggaaagt
     7501 gttgcggttc ggacggcatg gttttgaaat atcatgcagt ccgaacgtgg aattatgcgt
     7561 tcaaaacgag gacggcttcg gcttcgacct gcacgccttt gggcagcgag gcaacgccga
     7621 cggcggcgcg ggcggggaag ggctcggcga taaattccgc catgacttcg ttgaagacgg
     7681 caaaattgcc caagtcggtc aggtaggcgt tgagtttgac gatgtcggcc agcgtgccgc
     7741 ctgccgcttc ggcgacggct tgcaggtttt ggaacacttg gcgcgcttcg gcgcggaaat
     7801 cgccgttgcc gacgacggtc atcgtggcgg gatcgagggg gatttgaccg ctcatgtaaa
     7861 cggtgtcgcc tgctcggacg gcttggctgt acgcgccgat ggcggcgggg gctttgtcgg
     7921 tgtggatgat ggttttggac atttcggatt cctcaaaaaa tagggcggca gaagccgcag
     7981 cattcgggat tatcgtacaa aaccgccggc ttgtgtagtt gcggtggcag aaaacaaaac
     8041 cgccgaaggc tcggcggttt gcagaataag gcgcatatca gaatttgacg cgcataccgg
     8101 cggacagttc gccggaacgg acgtttttga cagtgttgac tttgccgatg tagttgtagc
     8161 ggtagccggc atccaaatcg acattcgggg taacggcata gcttacgccc gccaatacgc
     8221 cgaggccggt ggaggtttgg ctgaagctgt cgctgccgcc caagtcgacg gaggcgcggt
     8281 tgaggctcaa gcgcgcgccg agatacggtt tgacgggcga ttgggtgtcg aagtcgtaaa
     8341 tggcggacgc gccgatgctg taaagtttga aatcggtgga tggggcttta tagtttttgt
     8401 agcgcgtgta atcgacggcg aagcggaggt cgttgatgcg gtagcctgcg gagatgcgcg
     8461 ggctgaagcc tttggcagaa cctaaagagc ttgaggcttt tgcgtgtgcg gcatcggctt
     8521 ggacgtaaaa gctggatgcg ccttccgcca gtgcggcggc cgggagagcg agggcaatca
     8581 gtgcggcaag tgcttttttc atattttggt tcctttatgg tcagttagaa aaattgttaa
     8641 gaatccgtta aagaatcctg ctgtattata cttaaatttt cttttgcatc gtaatatttt
     8701 caatacttca agatacgtag cggtatccgg ctgctttgcc gacggcaaag ccgttaaccc
     8761 gcgcgttgcc tttaaatggt ggcggcggca tcacgcggcg gatgggtgaa acttgcaaac
     8821 ggtttggaaa aaacagcggt atctgtcgga ttgttgcagg tgcaggcata cggttttgtg
     8881 tgcgtctgtg ccttaagcgt cggacatttc cggcggcggc tgtgccgtct gaaacgcccg
     8941 gcgggggatg cggctgcgtt ttccatcgat aagcatattt tccggacgcg ttcggggcgg
     9001 gttttcccgg gcggccgccg atttgtttgc g
//
LOCUS       DAXHTR010000088         8853 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.088, whole genome shotgun sequence.
ACCESSION   DAXHTR010000088 DAXHTR010000000
VERSION     DAXHTR010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8853
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.088"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(53..247)
                     /locus_tag="ACFNML_001403"
     CDS             complement(53..247)
                     /locus_tag="ACFNML_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFC6392772.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(237..2318)
                     /locus_tag="ACFNML_001404"
     CDS             complement(237..2318)
                     /locus_tag="ACFNML_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HFC6392773.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANRVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPTLQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            2794..3174
                     /locus_tag="ACFNML_001405"
     CDS             2794..3174
                     /locus_tag="ACFNML_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HFC6392774.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDGGGRGHMPPVQNQAGTDDF
                     RAFSCENGLSVHVRRLDGGRIALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3307..3378)
                     /locus_tag="ACFNML_001406"
                     /pseudo
     CDS             complement(3307..3378)
                     /locus_tag="ACFNML_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713843.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
     gene            3829..5262
                     /locus_tag="ACFNML_001407"
     CDS             3829..5262
                     /locus_tag="ACFNML_001407"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFC6392775.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFLIKENKEALARLMTMEQGKGLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALAVLAYEAGVPQDLLPVVSGSASEIGREFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGTLASKF
                     RNSGQTCVCTNRVYAQSGIYEEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALSKGAVCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            5371..6159
                     /locus_tag="ACFNML_001408"
     CDS             5371..6159
                     /locus_tag="ACFNML_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HFC6392776.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALAYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDRWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            6527..8083
                     /locus_tag="ACFNML_001409"
     CDS             6527..8083
                     /locus_tag="ACFNML_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HFC6392777.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDSTTALGAIELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELAPQLNLPQQQEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8100..8846
                     /gene="surE"
                     /locus_tag="ACFNML_001410"
     CDS             8100..8846
                     /gene="surE"
                     /locus_tag="ACFNML_001410"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFC6392778.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEQALWTLLAHFFKNPPQSPILWNIN
                     IPAVEPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAVFWHAD"
BASE COUNT         2069 a         2225 c         2558 g         2001 t
ORIGIN      
        1 tataaaaatt ccgtctgaag cggcgttcag acggaatttg tttcaagcag gcttaacagc
       61 agcgtccgcc gtttgcgccg cagcggcgtt tgcggcaata gtcttgaaac tgcagcttgg
      121 tcatcacggg ggcgttggga ttatgtttgc gctgctgtgc aacgtagttt tcataatcgg
      181 gcacgcctgc catcaagttt gccgtcagct tgatggtttt ccaccaagac gcgagcttat
      241 gcttcacttt gtgcctccgg ctgtttgccg tcgcggtaca ccgccgggat ttctttggcg
      301 gtcggccagc cgactttgcg tgctttgagg gcggtacgca aaccgtacgc ggcgacaatc
      361 acgacaaccg acaagaagag gatggtcaga ccggcattaa tcttgtcgtt gaagatgatt
      421 tgcgccattt cgccgatgtc tttggcaggc gcaaggactt cgtttttagc caatgcgtcg
      481 ctgtatttgc cggcgtgggc aaggaagctg atgcgcgggt cgctgtggaa cagtttttgc
      541 aggccggcgt agcaggttac gaacagtacg ccgacggcgg gaacgagtac cacccagaca
      601 taacggtcgc gtttcatctt aatcagcacc acggcgcaca taatcaaggc tacgcctgcc
      661 agcatttggt tggcgatgcc gaacaaaggc cagagcgagt tgatgccgcc cagcgggtcg
      721 gttacgccgg tgtagagaaa gtagccccac aatgccacgg cgaagaaggt cgcaatcagg
      781 ttggcgggga tggagtcggt gttgccgaaa ggtttgtaga agatgctgcc caagtcttga
      841 atcataaaac gtgcgacgcg cgtaccggca tcgacggcgg tcaggatgaa caaggcttca
      901 aacaataggg cgaagtgata ccagaacgcc atcatcgcct cgcccggaat caggcggctc
      961 ataatgtgcg ccataccgac tgcgagggtg ggcgcaccgc ctgcacggga aaggatggtg
     1021 ttttcgccca cttccttagc ggtgtgcaac agggttgcag catcgacagg gaattgcagc
     1081 ttggtggtaa tcacttcggc ggcggtattg gcatccgtac cgatcagggc ggcagggctg
     1141 ttcatggcga agtacacgcc gggatcaagc gatgcggcgg cggcaagtgc cataatggct
     1201 acgaaacttt ccatcaacat accgccgtaa ccaatcatac ggacgtgggt ttcgttttcc
     1261 agcattttcg gcgtggtgcc ggaggaaatc agcgcgtgga agcccgaaac cgcaccgcag
     1321 gcgatggtga tgaagaggaa cgggaacaat gcgcctgaga ataccgggcc ggaaccgtcg
     1381 ataaagtggg ttacggcagg catttgcaaa gtgggattga cgatgacgat gcccaaagcc
     1441 aaggccgcaa tcgtaccgat tttcaggaag gtggagagat agtcgcgcgg agtgagcagc
     1501 agccatacgg gcaatacgga ggcgacaaag ccgtaaatca taatcgccca agtgagctgg
     1561 atgccgtcaa ggtcgaacca atgtccgatg gaacttttcg ccacatcttc gccgtaaatt
     1621 accgccagca tcagcaaaat aaagccgacg atggaaatct cgccgatttt gcccggacgg
     1681 atatagcgcg tgtaaatacc cataaacagg gcaatcggca tagtggcggc gatggtaaac
     1741 gtaccccaag ggctgtgaac cagtgctttt acgacaatca acgccaacac cgccataatg
     1801 atgaccataa tcatcaaaat accgatggag gcaatcacgc cggggacagt gccgagttcc
     1861 tgtttcacaa tatcgcccaa agacttaccg tcgcggcgca tagagacgaa caagaccatc
     1921 atatcctgta ccgcgccggc aaacaccacg ccgaagataa tccacaaagt accgggcagg
     1981 taacccattt gcgccgccaa aaccgggccg accaaagggc ccgcgcccgc aatcgcggca
     2041 aagtggtgtc cgaacaatac gcctttgtgc gtcggaacgt agtccaagcc gtcgttgtgg
     2101 cgttctgccg gagtcaggcg gttaggatcg agccgcatta cgcggttggc gatgtagagg
     2161 ctgtaaaaac ggtaggcgat gcagtaaacg gatatggcgg cggtgaccat ccataccgcg
     2221 ctgacctgtt cgcctcggct gagggccaga gtggtaaagg atgctaagcc gaccagtacc
     2281 actatgcccc aaatgaggaa ggttttgagt gatttcatcg aataaatcct tatctcacac
     2341 tgtcggaata tgcctgaacg gcgggaaacg gatgaccgcc tgccggcacg ggcacaaacc
     2401 ggagtctgaa tgattgacga agtatgaaac agtgcgcttg tcgggatttg tgccttatgc
     2461 ccgcatcaaa cagtgcaggg atgcgggcgc acaaaacgtt aaacgccgaa taggatttta
     2521 acgcaaatta gcacactgat agcggttttt acttggaatt tggaaaaaat ttacattcct
     2581 tcgggcggac aggcgggttt ggacggcatc gtcaggcaaa aggcggtgcc ggaagacggg
     2641 taaagaaggg gcgcgcaatc cggattattg attcatcgcg ggtaaattcc ggttatcggg
     2701 cttgtgtgtt tgcgcgtccg tttatagtat ggcgttgccg cagcttggaa tcagggcggt
     2761 tgtttcatat cttattttta ttggggagct tttatgaata tcaggttttt cgcgctgacc
     2821 gtaccggttt tgtctttggc ggcctgtgcc gtgccggaag cgtatgatgg cggcggacgc
     2881 gggcatatgc cgcctgttca aaaccaagcc ggcacggacg attttcgggc gttttcctgc
     2941 gagaacggtt tgtctgtgca cgtccgccgt ttggacggcg gcagaatcgc gttgcggttg
     3001 gacggcaggc gtgccgtcct ctcttccgac gttgccgcat ccggcgaacg ctataccgcc
     3061 gaacacggtt tgttcggaaa cgcaaccgag tggcaccaga aaggcggcga agcctttttc
     3121 ggctttaccg atgcctacgg caattcggtc gaaacttcct gccgcgcccg ctaaacgggg
     3181 cagcctgttt gaaccgccgt ccggattttt cgggcggttt tttgtgtcgg atttgttttg
     3241 gctgtcggct ggtttcggcg gttttcgggc gtggcggatt atttttcgtt tcgttccggg
     3301 tttatcctat cggcagcggc tcaatgccaa ctttaaacct gctccgattt cttcagggct
     3361 gttatccaat gataaaatat agtggattaa caaaaatcag gacaaggcga cgaagccgca
     3421 gacagtacag atagtacgga accgattcac ttggtgctta agcaccttag agaatcgttc
     3481 tctttgagct aaggcaaggc aacgctgtac tggtttttgt taatccacta taatgtttgg
     3541 atttttgcca tactctacca cacgttgcaa ctgcaatctt tgttccttat atattattaa
     3601 agggctgcat tacttttctt tctaaattac cattgtataa aaagtatttt tcttaaacga
     3661 cagggattgc gcccgctcca tattcagaca ccgcccggat gttgcgctgc ccgatcggat
     3721 gcttcagacg gcatcggaag ggtttgcagt tttggaatat gaagatgata atgtccgcga
     3781 gattgacggc atttgaaagc ggttattccg cataccggag gatacgaaat gaacgaatat
     3841 tcccaattaa tcaagcatcc cgatatttcc cttcccccgg tttcagacgg catcaaagtc
     3901 gataatccgg caacgggcga gactttggcg tttgtccgca agacggattc ggacaagctg
     3961 aaaaacctga ttcaaaaggc ggcggcagcg caaaaattat gggcggcaaa aaccgcgttg
     4021 gaacgcgccg atgtgttgtg gcgttggtat tttttgataa aggaaaacaa agaagcactg
     4081 gcgcgtctca tgacgatgga gcagggcaaa ggcctgaccg aggcgcgcgg cgaaatcgat
     4141 tatgcggctt cgtttgtgcg ctggtttgcc gaagaggcgc ggcggattga cggcgatgtg
     4201 ctgacgagtg tgaaagcgtc gcaaaaactg gtcgtgttga aacagcctgt cggtgttacc
     4261 gccgcgatta cgccgtggaa ctttccgtcc gcgatgattg cgcgcaaggc tgcgccagct
     4321 ttggcagtgg gttgcgcgat gatagtcaaa cccgcgtcgc tcacgccttt gagcgcgtat
     4381 gcgttggccg tgttggctta cgaagcgggc gtgccgcagg atttgctgcc tgttgtcagc
     4441 ggcagtgctt cggaaatcgg ccgtgaattt gccacaaatc cgattatccg caaaatcagt
     4501 ttcaccggct ctaccgaagt cggcgcgaaa atttttgccg acagcgcggc agacattaaa
     4561 aaactcagct tggaactggg cggcaacgcg ccgtttatcg tgtttgacga tgccgatttg
     4621 gacaaagctg tcgaaggtac acttgccagc aagttccgta atagcggtca gacctgcgtc
     4681 tgcaccaacc gcgtttacgc tcaatccggc atttacgagg aattttgccg caaattgagt
     4741 gaaaaagcag ccgcgctcaa attgggcaac ggcttggagg atggcgtaaa ccaagggccg
     4801 ctgattgagg aaaaagcggt agaaaaagtc gagcagcaca tcgccgacgc gctctcaaaa
     4861 ggcgcggtct gtctgaccgg cggcaaacgc agcgcgttgg gcggaacgtt ttttgagccg
     4921 accgtcttaa gcggtgtaac ggcgcaaatg gcggtagctc gcgaggaaac tttcgggcct
     4981 ttgtgtccgg tattccgttt tgaaaccgaa gccgaggtca tcgaggctgc gaacaatacg
     5041 gaatacggtt tggcagctta ccttttcacc gccgacaccg cccgccaatg gcgcgtcggc
     5101 gaagccttgg aatacggcat ggtcggcatc aatacgggct taatcagcaa tgaagcggca
     5161 ccgttcggcg gcgtgaaacg cagcggtttg ggacgtgaag gcagcaaata cggtgcggac
     5221 gaatatctag aattgaaata tttgtgtatg gatgtcgggt gagggatgcc atccgaacgg
     5281 cagttcgccg gactgtgccg cattttaagc agtctctatc tgttgtacaa tgcgccctgt
     5341 ttttacggtt atttttgatt tgaacaagat atgatggaaa acggaaaaac agtcccgaga
     5401 tggcgttttg ccttgaaaag tgcgggctgg cacctcttaa tcagcctatc ggttgcagga
     5461 ctggcggcat tgctggtttt taaggtttgg tatccttatc cttatgccga gctgacggga
     5521 gggctgtcgc tttatcagct ggtggtggct gtcgatatcg tatgcggccc gctgctgact
     5581 ttaattttgg caagcccgaa gaaaaagaca aaggcacgca tggtcgattt ttccatggtc
     5641 ggcatcatcc agctggcggc tttggcgtac ggtctgcaca gcgtttcgct ggcgcgtcct
     5701 gtggtggaag cgtttgaaca ggatcgtatg accattgtta cggcggcgga agtcgtggtc
     5761 gaagatttgc acaaagcccc cgaagggctg caaagcctgt cgtggttcgg catccgccgc
     5821 attgcattga aagaacctga ggatgcggat gagaagaaca agacgctgga tttgtccctg
     5881 aaaggtatcg agccgagtat gcgtcccgac cggtggctgc cgtattccga caaggaagca
     5941 gaagaaatcc gcaaacatct gaaaccgctg aaagtcttgg cggatgcgag aaaaacgacg
     6001 gttgcggaca ttctgaaaca ggcaggtctc gccgaagggg aggagctgta ttacctgccg
     6061 tttaccagca gcaggcagaa agagtggata gtcattaccg ataaagaggg caacaccaaa
     6121 ggctacgcgc cgatagacgg cttcatcatc accccttaag cgttgggact ccgtccgcac
     6181 tcgaacatcc gttcttcgcg gcggtagaat cagactgtat ttgagagagg cgcaacccct
     6241 caatcagggc aacacatagg ggaaggcttt atgtgccgtc cgtgtgttga aacatttcaa
     6301 aataagctca tgccgtccga acatcctttc agacggtatg gcgttttgcc attgccccga
     6361 taagctgtta aactatttaa attatttcga ccgaaggtac acgcacatgc aagaccccga
     6421 gcagagcagc aagcccgccc gccgtttttt gtgcgtaccg tctgttcagg cagcttgagc
     6481 gcttcaggct gccttttcgc gtttgaactt gaggaatacg aaaatcatgg atttcagttg
     6541 gttggcagaa ccgcatacct ggataggttt tgccacgctt ttggtgttgg aagtcgtatt
     6601 ggggatagac aatcttgtct ttgtggcgat tttggcaaac aaggtccagc ccgcacggcg
     6661 cgaccgcgca cggattatcg ggctggggct ggcagtcgtc atccgcatca ttatgcttgc
     6721 ttttatggcg cacatcatca cgctgaccga gccgctgttc caaatcggcg gcctcgccgt
     6781 ttccggcaag gacatgatta tgctcgcggg cggtattttc ctgctttaca aagccaccac
     6841 cgaactgcat gaacgcctcg aagggcacaa ccgttttacc gttgccgaca gccaaaaaaa
     6901 acacgcgccg ttttggggcg tggtcgcgca aatcctgata ctggatgccg tgttttccat
     6961 cgattcggtc attactgcgg tggcgatggt cgatcatatc gtcgtggcga tgggtgcggt
     7021 cgtcgtcgca atggctgtaa tgatttctgc cagcaaactc ttgaccgaat ttgtcgacag
     7081 acaccctacc gtcgtgatgc tctgccttgg ttttttgttg atgatcggtt tcagcctgat
     7141 tgccgaagcc ttccatttcc acattcccaa aggctacctc tacgccgcca tcggcttctc
     7201 gattttaatc gaactgttta accagatttc ccagcgcaac agccgcaaaa acgactacat
     7261 cggcagctcg tggcgcaaac ggactgctga aaatgtcttg ggtatgatgg gcatacgcga
     7321 aagcgtgctt gccgatgcgg gcggcgaatc tggggacgac gcgcattttg aagaaaacga
     7381 aaaatcgatg atacgcagtg tgctgacgct tgccgaacgc ccgattatgg gggtgatgat
     7441 cccacgccgc gacatcgaac ggctggacat ttcccaaagc cgcgaagaac agtatgccca
     7501 actgcaaaac acgccttaca gccgcctgct cgttgtcgga aaggcgggcg tggacgaacc
     7561 tttgggctac atcaacaaaa aagacctgct gtcccaactg ctggaaacag gcggtctcga
     7621 cattcagacg gcattgcgcc agccgctcgt tttgcccgac agcactaccg cgctgggcgc
     7681 aatcgaactc ttccgccaaa gcagcgcgga ttatgctttg gtggtggacg agttcggcgc
     7741 ggtattgggc atggtaacca tgaaagacct gctcgaaacc atcgcaggcg agttccccga
     7801 agaatttgag cgcgaagaag aaccagccgt tcaggggaat cccgatgaaa gcctgacggt
     7861 ggaaggcgcg ttggaatatg tggaactcgc accgcaactc aacctgccgc agcagcagga
     7921 agatgccgat ttccatacgg ttgccgggct gattatggaa gaattgcaaa ccatccccga
     7981 tgtcggcgat tttgccgatt tccacggctg gcggtttgaa gtggtcgaaa aagaagggca
     8041 gcgcatcgag cgggtcaaaa tcaccaaatt gcccgaagaa taagcattca ggatagaaaa
     8101 tgaacgtttt gatttccaac gacgacggct acctctccga aggtattgcc gttttggcgc
     8161 gcgttacggc ggaatttgcc aacgtcaggg tggttgcgcc cgaacgcgac aggagcgggg
     8221 tcagcaattc gctgacgctg gaacgccctt tgcagttgaa acaggcgcaa aacgggttct
     8281 actatgtcaa cggcacgccg accgactgta tccacatcgg gcagtctgta ttttcggatt
     8341 ttcaggccga ttttgtcttt tcgggcatca accggggcgc gaatatgggg gacgacacgc
     8401 tttattcggg gacggttgcg gcggcaaccg aagcctacct tatgggcata cccgctgtgg
     8461 cgttttcctt aaacgacgct tccggacgct attgggcgac cgcagaacag gcactgtgga
     8521 cattgttggc gcattttttc aaaaaccccc cgcagtcccc tattttgtgg aacatcaata
     8581 tccccgccgt cgagccggaa gatgtgcggg gcattaaaat cgcccgtttg ggcaggaggc
     8641 atcacggtca gaacgtcatc cccgcgcgca atccgcgcgg cgaacagatt tattggatag
     8701 gcccggtagg cagggtttcc gacaaagaag agggaacgga tttcggtgaa tgcggcgcag
     8761 gtttcattac cgtaacgccg ctgcaaatcg acctgaccgc ctatccggac atggcggaaa
     8821 cagcggtgtt ctggcatgcg gactgaccgt ttc
//
LOCUS       DAXHTR010000089         8726 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.089, whole genome shotgun sequence.
ACCESSION   DAXHTR010000089 DAXHTR010000000
VERSION     DAXHTR010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.089"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(241..3093)
                     /gene="gcvP"
                     /locus_tag="ACFNML_001411"
     CDS             complement(241..3093)
                     /gene="gcvP"
                     /locus_tag="ACFNML_001411"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFC6392779.1"
                     /translation="MKLSELFNPDEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNMINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAQADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVSADIMSLVLLKPPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDAFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIAKRIHALASAFADALVSDGIKVVHKVFFDTVTVDFGSK
                     EKTDQVFAAALESGYNLRRVNDTQVAAAFHETSTREDLVDLYRAFTGKDTFAFADDVK
                     GRLNAELLRQNDILQHPVFNSYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLADMENSLKAITGFDAISFQPNSGAQGE
                     YSGMLSIRRYQEANGEGHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAKVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHTLTDTHSASADQT
                     AVAAAAYGSASILPITWMYLTMMGKQGMEQATRWALLNANYVAKRLGEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKSIRREVQKVIDGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPFVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(3124..3483)
                     /locus_tag="ACFNML_001412"
     CDS             complement(3124..3483)
                     /locus_tag="ACFNML_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392780.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRCANLMAVLPL
                     FVVILVLLNIVFPLPAHPLAWLVPAGFMVLVGGFPLSLPLAVLLVLTCCILAHCPPLS
                     RLLCYPCPNHPMSKNSA"
     gene            3482..3706
                     /locus_tag="ACFNML_001413"
     CDS             3482..3706
                     /locus_tag="ACFNML_001413"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HFC6392781.1"
                     /translation="MPSEPAGRLFQKMPYRCNFMRLYGVGFALQTAYASRSSLVSQSV
                     HDYSGLNLNQYGVASPCRTICTVCGFVALS"
     gene            complement(3758..4399)
                     /locus_tag="ACFNML_001414"
     CDS             complement(3758..4399)
                     /locus_tag="ACFNML_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HFC6392782.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARTHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(4389..4940)
                     /locus_tag="ACFNML_001415"
     CDS             complement(4389..4940)
                     /locus_tag="ACFNML_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFC6392783.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIEDDNELFERLVLEI
                     NQAGLSWTLMLKKRQAFQTAFEGFDIDTVAAFGEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            complement(4944..5462)
                     /locus_tag="ACFNML_001416"
     CDS             complement(4944..5462)
                     /locus_tag="ACFNML_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFC6392784.1"
                     /translation="MRLFPIAAALTLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            complement(5462..7123)
                     /gene="pqiB"
                     /locus_tag="ACFNML_001417"
     CDS             complement(5462..7123)
                     /gene="pqiB"
                     /locus_tag="ACFNML_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFC6392785.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWVKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     DLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSASPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            complement(7116..8576)
                     /locus_tag="ACFNML_001418"
     CDS             complement(7116..8576)
                     /locus_tag="ACFNML_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiA/YebS family transporter subunit"
                     /protein_id="HFC6392786.1"
                     /translation="MGSAVGGQPLEKIFLPDDKGLFILPNLPVLHPDWVYRLFSFIMP
                     PLIFATFPMKPFAENIPHSLRGNCRDEALPPHTVDCPECGCRADVPRLDSGEAAFCPR
                     CGYKLFRVGRHPFSAPPAYAAASLILMAFAYGMTYIEVGIPGAASVLSLPEMMRLMVF
                     QDYGFLAEVMFVLTFGAPVLFLLLCLYVYAALIRKRVYPALRLATRVMVRLRQAMMVD
                     VFFVSTLVAYIKLSSVAEVRFGSAFYLMFALSVMLIRTSVSVPQHWVYFQIGRLTGDN
                     AVQTASEGKTCCSRCLYFRDGAESPCGVCGAELYRRRPKSLSISSAFLTAAVILYFPA
                     NILPIMISSNPAATEVNTILNGIAYMWDEGDRLIAAVIFSASILVPVLKIAAMSVLIA
                     SARFALPAGAKKLSHLYRITEAVGRWSMIDIFVIIILMCSFHTYAARVIPGSAAVYFC
                     LVVILTMLSAYYFDPRLLWDKRASDGMAFNETEKHD"
BASE COUNT         1947 a         2229 c         2474 g         2076 t
ORIGIN      
        1 gtttgtccgt gtggaaatga catcaaaggg tgtgaatagg cttttcatct ttgaaatctg
       61 tctgcccgat cggatgaata aaaagtagga tgataaaaaa aactgatgta tggatactgg
      121 tttaacccat ttgttttaaa gttatagtcg agatacgtgt atcggtgcaa cgagtctgcc
      181 gtcgacgaaa atgccgtctg aaagtgtttc agacggcatt tgtctttggg gtttcaacgg
      241 tcagtcttca taattttcca tcggtgggca gctgcacacg agattgcggt cgccgtacac
      301 gtcgtccacg cggttgacga agggccagaa tttgtgttcg cgcacgaagg gcagcgggaa
      361 gacggcttct tcgcgagagt acggatgcgc ccattcgccg gttacgtcgg aggcggtatg
      421 cggagcattc accagtgggt tgtcctcttt cggccattcg ccgtcgatga ctttctgcac
      481 ttcgcggcga atggatttca gggcggcgat gaagcggtcg agttcggctt tgctttcgct
      541 ctcggtcggc tcgatcatca gcgtaccggc aacagggaag gaaaccgtcg gcgcgtggaa
      601 gccgtagtcc ataaggcgtt tggcgatgtc ggtttcggtg atgccgcttt cggctttgag
      661 cggacgcaaa tcgacgatgc attcgtgcgc gacgcggccg tttttgccgg tgtagagaat
      721 cggatagtct tcgcctagac gtttggcgac gtagttggcg ttgagcaatg cccaacgtgt
      781 cgcctgctcc ataccttgtt tgcccatcat ggtcaggtac atccaagtaa tcggcaggat
      841 ggatgcagaa ccatatgctg cggcagccac ggcagtttga tcggcacttg cgctgtgggt
      901 gtcggtcaag gtatgacccg gggcaaacgg ggcgaggtgg gctttcagac cgatgggacc
      961 catgcccgga ccgccgccgc cgtgggggat gcagaaggtt ttgtgcaggt tcatgtgcaa
     1021 cacgtctgcg ccgactttgg caggctgcat gatgccgatt tgggcgttga ggttggcgcc
     1081 gtccatgtaa acctgtccgc cgttttcgtg gacgatgcgg cagatgtcgc ggatgccttc
     1141 ttcgtacacg ccgtgggtgg acggataggt aatcatgatg gcggacaaag cgtcgcggtg
     1201 ttgctcggct ttggctttca aatcgtcgat attgacgttg ccgtgttcgt cggtatcgac
     1261 aacgacgact ttcaagccca gcatggccgc agtcgcaggg ttggtaccgt gggcggattt
     1321 gggaatcaga cagatgttgc ggtgtccctc gccgttggct tcctgatagc ggcggatgga
     1381 gagcataccg ctgtattcgc cctgcgcgcc ggagttgggc tggaaggaaa tcgcgtcaaa
     1441 gccggtgatg gctttcaggc tgttttccat gtcggcaagc aattcgcggt agccggcggt
     1501 ttgcgcttcg ggagcgtaag ggtggatgtc ggagaactcc gcccaagtaa tcggcaacat
     1561 ttccgcagtg gcattgagtt tcatggtgca actgcccagc gaaatcatac tgcggttcat
     1621 cgccaaatcg cggtcttcga gttttttcag gtagcgcaac atttcgtgtt cggtgtggta
     1681 actgttgaac acaggatgtt gcagaatgtc gttttgacgc agtaattcgg cgttcagacg
     1741 gcctttgaca tcatcggcaa atgcgaacgt atccttgccg gtaaacgcgc ggtacaaatc
     1801 aaccaaatct tcgcgtgtcg aagtttcatg gaaggctgct gcgacttgag tatcgttgac
     1861 gcggcgcagg ttgtaacccg attccaaagc agcggcaaac acttggtctg ttttctcttt
     1921 actgccgaaa tcgacggtaa cggtatcgaa gaagactttg tgaaccactt taatgccgtc
     1981 tgaaaccagc gcgtcggcaa aggcagaagc cagcgcgtga atgcgcttgg caatacgttt
     2041 cacaccttcg gggccgtggt aaacggcata catacccgcc aagttcgcca gcaatgcctg
     2101 tgccgtacaa atattggatg tcgctttttc gcggcggatg tgttgctcgc gggtggacaa
     2161 cgccatgcgc aaggcaggtt tgcccgatgc gtctttggat acgccgatga tgcggcccgg
     2221 ggcggaacgt ttgaacgcgt ctttaaacgc gaaataagca gcgtgcggac caccgaagcc
     2281 catcggtacg ccgaagcgtt gcgtgttgcc caaagcaata tccgcgccca attcggcagg
     2341 cggtttcagc aaaaccaagc tcataatgtc ggcggaaacg gcaacgattg tgccttttgc
     2401 tttcagacgg ccgataacgt cctgcaagtc ttgcacatcg ccgtctttgc cgacgtattg
     2461 gaacagcgcg ccgaagtatt cgccctcgtc ggcttgagca aaatcgccga ccaccagctc
     2521 gaagccgaaa tacttggcac gggttttcat cacgtccaaa gtttgcggat acacgcgctc
     2581 gtccacaaag aaacgctcgg attttacctt gcccacgcgg tgcgccatcg ccatcgcttc
     2641 ggcggcggcg gtcgcttcgt ccagcaaaga cgcgcccgcc acagggaaac cggtcaaatc
     2701 gatacacact tgttggaagt tcaacaaagc ttccaaacga ccctgcgcga tttccgcctg
     2761 atacggcgtg taggcggtgt accaacccgg attttccaat acgttacgca aaatcacgtt
     2821 cggcacgcgg gtcgggtaat agcctaaacc gatataggat ttgttgatca tgtttttcga
     2881 cgcaatgcct ttcaattttg ccaacgcgtc cgcttcggtc agggcatcgg gcaaatcgag
     2941 ttcagacggc atacggatgc tttgcggcac ggtgttgccg acaaaatcgt ccatactttt
     3001 ctcgccgaca gcggcaagca acgccgcttc gtcgccaaaa ctcaaatgcc gcgcggcaaa
     3061 ttcgtcgggg ttgaacagtt cggacagttt catttctact ccttcgggtt tgtagggctg
     3121 ccatcaggca gaatttttag acatcggatg attcgggcaa gggtagcaca aaagacggga
     3181 taatggcgga caatgcgcca gaatgcagca ggtcaggaca agcagcaccg caagcggcag
     3241 cgataaggga aagccgccga ccaaaaccat gaaacctgca ggcaccagcc aagctaaggg
     3301 atgcgccgga agcggaaaaa caatattgag cagaaccaaa atcacgacaa acagcggcag
     3361 caccgccatc aggttggcac acctgtatgt tgcgtacggg tcggacgctt cttcaggttc
     3421 gcaatattcc atgatttcag cctgtccggg ctttatccac agcaggctcg gacggtcggt
     3481 catgccgtct gaaccggcag gccgtctttt tcaaaaaatg ccataccgct gcaacttcat
     3541 gcggctgtat ggcgtaggtt ttgcacttca gacggcatat gcgtcccgaa gcagtttggt
     3601 gtcccagtcg gttcatgatt atagtggatt aaatttaaac cagtacggcg ttgcctcgcc
     3661 ttgccgtact atttgtactg tttgcggctt cgtcgccttg tcctgatttt tgttaatcca
     3721 ctatatataa acaacttggg tgcggataaa cctttgttta tctaaacccc tgttttctca
     3781 aaaattgatt caaatcttcg gcaaatttcc gatagccttt gccgttggcg tggatttggt
     3841 cggatttcag attattatcg cccaaaattt ccgcccacgc gccgccgaac agcggaatgc
     3901 cgtattcctc ggacaaatcc tcatacagcg gatgatcgct caaatgcccg aacaacgcgc
     3961 ccaaggtaat gtgcggcacg ccgacgagga cggcggggat gttttccttc tgcacggttt
     4021 cgatgatttt cgcgatattg gcgcgggtct gctccttggg aactttgcgc agaaagtcgt
     4081 tgccgcctat gccgacaatc acaagcttgg gtttgcgtgc caacagcgcg ggcaggcgcg
     4141 acagggcttg ggcggatgta tcgcccgata cgccgccgtt gacaatattc caacccgtca
     4201 gtttttgcag ttgcgcgggg taggattcgc cggggtttgc gccgtagccg aaggtaagcg
     4261 aatcgcccaa ggcaagtacg gtgcttcctt cgggaatttt ggcgtgggtt cgggcggatt
     4321 ttctgccgca ggcggtaagg agcaacgcgc ctgcgccgag gaggaaggtt cttctgttca
     4381 ttggtttttc agatggcatc gagccatttc gggtggtatt tcagggtttc acggtaaacc
     4441 ggacagcttt cggcgtgcgc gcctttgagg tagccggtac tcatcagaaa ttcgccgacg
     4501 atttcgccgc cgacgaattt gaaatgtttt ttaaagagtt taacccattc gtctttgctt
     4561 cgcggatggt gcgcgtcgag ccagttcttg aacgagccgt attcttgttg caacgcttgg
     4621 atttgccgtg cattgaaaat ggcggcatcg attttcaggc ggttgcggac aatacccgca
     4681 tcggcaagca gccgctcaat atcggcttcg ccaaatgcgg caacagtatc gatgtcgaac
     4741 ccttcaaatg ccgtctgaaa cgcctgccgc ttcttcagca tcagcgtcca gctcaatcct
     4801 gcctgattga tttccaacac cagccgctca aacaattcat tgtcgtcctc aatcggaaaa
     4861 ccgtattggg tgtcgtggta atgtttgttc gggttgtcgg tgttttcggg aagtgaggcg
     4921 gcaaattcgc aatagttcac ggtttactcg accatctgtt gcgccgcctg tttcagtccc
     4981 tgttcgagtg cggcggtcat ggcggcgtag ccgtcaccct gctgttcggt ttcgatatgg
     5041 aagggtctgt tcgtaccgtc gggtaggacg gcgtagccgc tgatgagggt tttgcccgtg
     5101 tagctgcctt ggaatgcgtc gatatagacc gtccattttt cggtactgcc gctgcgtgag
     5161 gcaggaacaa agatgcgtgt gctgtccaaa cggttgaatg cattgctcaa cgccgcttcg
     5221 agcatatcgt ccaaggtgtc tgcccagacg tggttttgtg cggtgttgag gcggtagggg
     5281 tcggtttgat agaccagtcc gccgcgtttg agcggttcgg caagacggac ttcgacggca
     5341 gtttcgccgc cttgcgttgc aggacggatg tagcggctgt cgggcaacac gaaatattgt
     5401 gtgctttgca cagtaccgca ggcggcaagc gtcagggcgg cggcaatcgg gaagaggcgc
     5461 attatcggct tcctttcggg atagggtctt tgctgctgct gttgaaaatc agcgcgttgg
     5521 gtttttcttt caaagtgtta atgacgggtt gaacgtcttt taaggttttg tccaaacttt
     5581 gcagcgtatt ttgtacgtcg ccgtagatag gcgattgagg cgatacgcct tgcagggttg
     5641 tgcgcaactc tttcagggtt tggttcagtt cgttcggaat gttttgtgtc tgcggtttgc
     5701 cgaccagttt gtcgatggag cttagggcgg cattggcaga tttgagtgtg gatttgagct
     5761 cggcaagcga accgttcaat tcggcaaccg tcttatctaa aggcagtttg tcgaacttgt
     5821 ccagcaaatc cgccaatttg acctgcaaat cgtccaaacc gccgccctgg gtcgcgataa
     5881 cggtatcgcc tgcataaacg gtatgcggtc gcagcttagg tgatgcggaa ggctgatcgt
     5941 tcaactcaat cattttgctt ccggtcagca ggttgttgct ggagatggtg gcggtcaggc
     6001 ctttgtttaa ggccgtctga aattgttgtt tccaatgttc tttgctttgt tcgtcggcat
     6061 tgatttccaa acgggaaggt tcaatgcgga tgcgtacggg tatccagccg ttttcaaaca
     6121 ggtgcaggct gtcgttgcgg tcgaaataag gaacgtcgga aaccacgccg acattcagcc
     6181 ctttgtactc gacgggcgaa ccgacggtca ggccgcgcac ggattgtttg aaaaacgcgg
     6241 tgtagtacag cgagcggtcg tcaggcaggt tggcgacttc gctgcggctg tcgtaaagcg
     6301 tgaagctgtc ttcgcttttg acgtttttac tgtttttggt tttcggcgaa tcaaatgaaa
     6361 tcgcgcccga cagcagggca ggcagagggg cggaattgag tttgatgccg ctgcctgtgg
     6421 tttcgatatt gatgccgctt tccagccaaa aacggctggc ggaatgaatc agtttgtcgt
     6481 tggggctttg gatgaagatg gtgtaatgca cgctttggtc ggacgggtcg aaatgcgcgc
     6541 tttcgacttg cccgaccata aagttttcat acaaaacagg gctgttgacg ttgaggatgc
     6601 ggtcgttttt accaatcaaa ttcaagcgca gcccgctttg cccgatggcg gtaacgggcg
     6661 gaatgtcctg cacctggaac acgtcttttg cctcgtcgct tttgccgggt gtaaaggcga
     6721 tgtacgaacc cgaaagcagc gtacccaaac cggttacgcc gctttggtcg atacgcggct
     6781 tgaccaccca aaactgggta tcgctgcgga tgaggtcgga tacgtccgca ttgagttggg
     6841 cggttacttc cacgcctttt tggtcgtcgc gcagtttgat tcgggtaacg cgtccgacat
     6901 cgatgctcaa tactttgatg accgtattgt tgacctcaat gccttccgcg ctgtccatca
     6961 agagcgtaac cacaggcccc ctgttgcgga tttccttaac ccaaagccag ccgccggcaa
     7021 tcagcgcgat cagcggaacc agccagacgg cggagagcaa ggtgttgttt ttgcggacgc
     7081 gtgcttgggc gtgtccgttt ggaggagggc tgttgtcagt catgtttttc cgtttcgtta
     7141 aaagccatgc cgtctgaagc gcgtttgtcc caaagcaggc gcgggtcgaa ataataggcg
     7201 gacagcatcg tcagaatcac gaccaggcag aaatagactg ccgcactgcc cggaatgacg
     7261 cgcgcggcat aagtgtggaa cgaacacatc aaaataataa tcacaaaaat atcaatcatc
     7321 gaccagcgac cgactgcttc ggtgatgcgg tagaggtgcg acaatttctt tgcgcccgcc
     7381 ggcaaagcga agcgggcgga cgcaatcaaa accgacattg ccgcaatctt cagcaccggc
     7441 accaaaatac tcgcgctgaa aataaccgcc gcaatcagcc tgtcgccctc gtcccacata
     7501 taagcgatgc cgttaaggat ggtattgacc tccgtggcgg caggattgga tgaaatcata
     7561 atcggcagga tattggcagg gaaatacaaa ataaccgccg ccgtcagaaa cgccgacgaa
     7621 atactcagac ttttcggccg tcggcggtac agttccgcac cgcacacgcc gcagggggat
     7681 tcggcaccgt ctcggaaata caggcagcgg ctgcaacagg ttttgccttc tgatgccgtc
     7741 tgaaccgcat tatcccccgt cagccgcccg atttgaaaat acacccaatg ctggggaacc
     7801 gataccgaag tccgaatcag cataaccgac agcgcgaaca tcagataaaa cgccgacccg
     7861 aagcgaacct ctgccacaga cgagagcttg atatacgcca ccagcgtgga aacaaaaaac
     7921 acatccacca tcatcgcctg cctcaagcgc accatcacac gcgttgccaa acgcagcgca
     7981 ggatacaccc gtttccgtat cagcgcggca tagacataca ggcacagcag cagaaacaga
     8041 accggcgcgc cgaaggtcag cacaaacatc acttcggcca aaaaaccata atcctgaaac
     8101 accatcaggc gcatcatctc gggcagcgaa aggacggatg ccgcacccgg tatcccgacc
     8161 tcgatatacg tcataccgta agcaaacgcc atcaaaatca gcgaagccgc cgcataagcg
     8221 ggcggggcgg aaaaaggatg cctgcccacc ctgaagagct tatagccgca gcgcgggcag
     8281 aacgccgctt ctccactgtc caaccggggt acatccgcgc ggcagccgca ttccggacaa
     8341 tctaccgtat gcggcggtag ggcttcgtcg cggcagttgc cgcgaaggct gtgggggata
     8401 ttttcggcaa acggcttcat cgggaaagtg gcaaatataa gtggcggcat tataaaggaa
     8461 aaaaggcgat acacccaatc aggatgcaaa accggcaggt tcggcagtat gaacaaaccc
     8521 ttgtcgtccg gcaaaaatat tttttcgagc ggctgtccgc cgactgccga acccatttga
     8581 taaaatcccg attctgtaaa gaatagtaaa atacctgctt aaaaacatca cgaaaatgat
     8641 attttttcca aaaatatgtg ataataaatt tcgtttatga ttctttatta tttttatagt
     8701 ggattaacaa aaatcaggac aaggcg
//
LOCUS       DAXHTR010000090         8542 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.090, whole genome shotgun sequence.
ACCESSION   DAXHTR010000090 DAXHTR010000000
VERSION     DAXHTR010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8542
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.090"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(1..277)
                     /locus_tag="ACFNML_001419"
                     /pseudo
     CDS             complement(1..277)
                     /locus_tag="ACFNML_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(556..855)
                     /locus_tag="ACFNML_001420"
                     /pseudo
     CDS             complement(556..855)
                     /locus_tag="ACFNML_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349130.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1072..1383)
                     /locus_tag="ACFNML_001421"
                     /pseudo
     CDS             complement(1072..1383)
                     /locus_tag="ACFNML_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1554..1757)
                     /locus_tag="ACFNML_001422"
                     /pseudo
     CDS             complement(1554..1757)
                     /locus_tag="ACFNML_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(1786..2115)
                     /locus_tag="ACFNML_001423"
     CDS             complement(1786..2115)
                     /locus_tag="ACFNML_001423"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFC6392787.1"
                     /translation="MGGLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLAIPSEMGYGAHGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(2193..2411)
                     /locus_tag="ACFNML_001424"
     CDS             complement(2193..2411)
                     /locus_tag="ACFNML_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HFC6392788.1"
                     /translation="MLKTLTFAALHFSTAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVQQTQPFQLHRCS"
     gene            2533..3486
                     /locus_tag="ACFNML_001425"
     CDS             2533..3486
                     /locus_tag="ACFNML_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HFC6392789.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIIITNKVMISADIIAHAPQLELIAVSATGVNNVDIEAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLARYAQAFGMRVVFAEHKHAPVVREGYVSFEDAVQTADVLSLHCPLNAQTENMIGEN
                     ELQQMKPGAVLINCGRGGLVDENALVAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(3561..5618)
                     /gene="metG"
                     /locus_tag="ACFNML_001426"
     CDS             complement(3561..5618)
                     /gene="metG"
                     /locus_tag="ACFNML_001426"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFC6392790.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECY
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTGFNIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLDECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVSGSALLAKLAAESDTIAEQYENREYARALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPILPKVAEN
                     AAKFLNLEAITWANTRETLGEHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAAPAA
                     EESQYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYLNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(5733..7571)
                     /gene="glmS"
                     /locus_tag="ACFNML_001427"
     CDS             complement(5733..7571)
                     /gene="glmS"
                     /locus_tag="ACFNML_001427"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFC6392791.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKNGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPTDVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSGEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGKLSPIVHTIPVQLLAYHTALARGTDVDKPRNLAKSVTVE"
     gene            7853..8371
                     /locus_tag="ACFNML_001428"
     CDS             7853..8371
                     /locus_tag="ACFNML_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392792.1"
                     /translation="MKQMLLAVGVVAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVFTGKEESMLLSEKDGALSINTGIGEIPIKLSDDGKELYVERRQYVKT
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQHQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
BASE COUNT         1768 a         2016 c         2538 g         2220 t
ORIGIN      
        1 cgcgcgtaac cggctgtccg cagaaccatt ttaccgaacc ggcttgacgc ttggcccaca
       61 gggagagttt tttgcctttg atttcattgt ttacgttgct tgaagccatt gtggcggtaa
      121 tgacgccgtt gttgacttca actttctgaa catatttgcc tttgatgtcg gcggaggatg
      181 ccacgccggc ggcactattg tcggatggcc atatgccgtg attcaggtaa tactctgtga
      241 cggctgattt ttgaccttcg accaaaagga tagcttcgtc attcccgcgc aggcgggaat
      301 ccagccttgt cggtacggaa acttatcggg taaaacggtt tctttagatt ttgcgttctg
      361 gattcccact ttcgtgggaa tgacgggatt aaagtttcaa aatttattct aaataactga
      421 aactcaacga actagattcc cgcttttgcg ggaatgacga agtttttcgg ccatgtgccg
      481 tgattcgggc aatactcggt aacggctgat tttttgaaaa tgtttgaaat cggcgcgtgg
      541 tgttcttgtg caaatggcag atgccgcatc gcggcaggtt gacggcaggt gtttggtgtt
      601 gattccgttg ttgctgttgt cgttggcggc ggtgacggcg tctttggcgg tgttgttggc
      661 ggcgcgcgta accggctgtc cgcagaacca ttttaccgaa ccgttttgac gcttggccca
      721 cagggagagt tttttgcctt ggatttcttt gtttacgccg cttgatttca tttcggcggt
      781 aacgacgccg tttttgactt caactttctc aacatatttg cctttgatgt cggaggcgga
      841 tgccacgccg gcggcatcat taaatcccgt cattcccacg aaagtgggaa tctagaactc
      901 agggccggag aaaccttttt acccgataag tttccgcacc gacaaaccta gattcccgcc
      961 tgcgcgggaa tgacgaagtt tttcggccat gtgccgtgat tcgggcaata ctcggtgacg
     1021 gctgattttt tggaagtgtt tgaagtcggc gcgtggtgtt tctatgcaaa cggcagatga
     1081 tgcgtcgcgg caggttgacg gcaggtgctt ggtgtcgatt ttgtcggtgc ccttgccggt
     1141 ggcggcggtg acttcgtcgt tgttggcgcc gttggcgttg gcgcgctgaa ccggctgtcc
     1201 gcagaaccat tttaccgaac cgtcttgacg cttggcccac agggagagtt ttttgtcttt
     1261 gatttctttg tttacgttgc ttgaagccat ttgggcggta atgacgccgt ttgcgactgt
     1321 aacgctttga acatatttgc ctttgatgtc ggagggggag gatgccacgc cggcggcatc
     1381 attaaatccc gtcattccca cgaaagtggg aatccagaac gcaaaatctc aagaaaccgt
     1441 tttacccgat aagtttccgt accgacagac ctggattccc gcctgcgcgg gaatgacgaa
     1501 gtttttcggc catgtgccgt gattcgggca atactcgggt ggtttgtgca accggcagtt
     1561 gattcatcgc ggcaggttga cggcaggtgc ttggggtcga tctgcttgtc gctggcggcg
     1621 gtaacggcgg tgtcggtgtc ggtgtcggcg cgcgtaaccg gctgtccgca gaaccatttt
     1681 accgaaccgt cttgacgctt ggcccacagg gagagttttt tgtctttgat ttcgttgttt
     1741 acgccgcttg aagccattat gtcagacggt attgcccggg cagctttatt cgtacacttt
     1801 cagcagctcg acttcaaata tcaaagtggc gtgcggggga atcacgccgc ccgcgccgtg
     1861 tgcgccgtag cccatttccg aagggatggc cagcttgcgt ttgccgcctt ccttcatgcc
     1921 gccgaagcct tcgtcccagc ctttgatgac ttgtccgaca ccgagcgtga tggtcagcgg
     1981 ctggcggcgg tcgaggctgg agtcgaattt ggttccgttt tccagccaac ctgtgtaatg
     2041 cacggtaatc tctttgcctt taactgcttc ttttccgaag ccttcttgca agtcttcaat
     2101 aatcaggccg cccatatttg tcctttcgtt gcttgttggt caaaacggca agggtaacat
     2161 accgtccgtc gaagtcaaat gccgctcaaa cgtcagctgc atcggtgcag ctgaaacggc
     2221 tgtgtttgtt ggactgtttt atttttttcg taaaggttcc atgctttttc atggaaatag
     2281 aaaacgacgg tgttgattag gggctcgacc agcgcgaccg cccccgatac gcctatactg
     2341 cccgtcagta cataggttac actgaaggcg gtgctgaaat gcagtgcggc aaaagtcagg
     2401 gttttaagca tcatcctctc ccgaattgga cattgacgga ggaatgataa aggttatcat
     2461 aaggttgcgc ggtttaaatt tgctatttgt tgttagtgta aataaatcat ttttcaaatg
     2521 gggatagaaa ttatgaatca taaaaagatc gttgttttgg atgcggatac tttacccggc
     2581 cgggtttttc attttgattt tccgcacgag cttgcggttt acggtacgac aggtgcggat
     2641 gaaacggcag aacgggtgcg cgacgcgcat attatcatca ccaataaagt gatgatttca
     2701 gcggatatca ttgcacacgc tccgcaactt gaattgattg ccgtcagtgc gaccggtgtg
     2761 aacaatgtcg atattgaggc ggcgaaggcg gcgggcgttg cggtatgcaa tgtccgcgcc
     2821 tacggaaacg aatcggttgc ggaacacgcc tttatgctga tgattgcgtt aatgcggaat
     2881 ttgcccgcct atcagcgtga tgttgcggca ggattatggg aaaagtcgcc gtttttctgc
     2941 cattatggcg cgccgattcg ggatttgaac ggtaaaacgc tggcggtttt cggacgcggc
     3001 aatataggac ggacgcttgc ccgatatgca caggcattcg gtatgagggt agtgtttgcc
     3061 gaacacaaac acgcgcccgt tgtgcgtgaa ggctatgttt cctttgaaga tgcggtacag
     3121 actgccgacg tgttgtcgct gcactgtccg ctgaacgccc aaactgaaaa tatgataggc
     3181 gaaaacgaat tacagcagat gaagcccggt gcggttttga tcaattgcgg gcgcggtggg
     3241 ctggtggatg aaaatgcgct ggttgccgca ctcaaatacg ggcagattgg cggggcaggt
     3301 gtcgatgttt tgacggagga accgcctagg ggcggcaatc ccttgctgaa tgcacgactt
     3361 cccaatctga ttgttacgcc gcataccgcg tgggcaagtc gtgaagcgtt ggacaggctg
     3421 ttcgatatat tgttggcgaa cattcacgcc tttgtgaaag gcgaggcgca aaaccgcgtg
     3481 gtttgaacct gtcgggattg cggaaaaaaa tgccgtctga acgcctcaag ggttcagacg
     3541 gcattttttg agatttctgc ttaaccgact ttgtcgcccg gttgcgcgcc tgcatccaca
     3601 tccaagagct tcagtttccc gtctgccgtg gcggcagaga gaatcatgcc ttcggatacg
     3661 ccgaattttg ccattttgcg cggggcgaag ttggcgacgg cgatgaccat ccttccgttt
     3721 aactcggcag ggttcaggta agacgcggcg atgccggaga agatgatgcg tttttcaaaa
     3781 ccgaaatcga ggtcgaattt caaaagtttg gtgctgcctt cgacagcttc gcagttcaat
     3841 actttggcaa cgcgcatatc gattttcata aagtcgtcga agcttgcctg ttcggcaact
     3901 ttttcgtatt gactttcttc ggcggcaggt gcggctgcgg cggcgatgct ttgtttgttg
     3961 gcttcgatta aatcgtccac ttgtttttgc tccactcgtt gcatgagatg ttcgtatttg
     4021 ttgatggcgt gttcgcccaa ggtttcgcgt gtgtttgccc aagtgatggc ttccaaattc
     4081 aggaatttgg cggcgttttc ggccactttc ggcaagatgg gggcaaggta ggcggtcagc
     4141 atggtaaagg cgttgatgag ttcgctgcat acttcgtgca ggcgttcgtc ttggccttct
     4201 tgtttcgcca gttcccaagg tttgttggca tcgacgtatt cgttgacaat gtctgccaag
     4261 gccatgatgt cgcgcagggc gcgggcgtat tcgcggtttt cgtattgctc ggcgatggtg
     4321 tcgctttcgg cggcaagttt tgccagcaat gcgctgcctg aaacgtcttt cagacggcct
     4381 tcaaagcgtt tggcgatgaa acctgatgcg cgggcggcga tgttgacgta tttgccgacg
     4441 aggtcgctgt ttacgcgcga aataaagtct tgcaggttca aatcgatgtc ttcgattttg
     4501 ctgttgagtt ttgcggcgat gtagtagcgc atccactcgg ggttcaggcc ttgttccaaa
     4561 taagatttgg cagtgatgaa ggtgccgcgc gatttggaca ttttttgtcc gtctacggtc
     4621 aaaaagccgt gtgcgtacac gccggtcggg gcgcggtggc cggagaaatg cagcatggca
     4681 ggccagaaca gggcgtggaa atagaggatg tctttgccga tgaagtggta catctcggtt
     4741 tggctgtcgg ctttgaagta ttcgtcaaaa tcgacgccga tgcggtcgca caggttttta
     4801 aacgacgcca tgtagccgac gggcgcgtcc agccagacgt agaagtattt gcccggcgcg
     4861 tcggggattt caaaaccgaa atacggtgcg tcgcgggaaa tgtcccagtc ggacagggtg
     4921 gtttcttcgc cttcgcccag ccattctttc attttgttga gggcttcggg ttgcagatgg
     4981 ggcttgccgt cgtgcggatt gttgccggaa gtccatgctt tgaggaagtc ggcgcattcg
     5041 tccagtttga agaagaagtg ttcggattcg cgcaattcgg gtttggcacc ggaaacggcg
     5101 gaatacggat taatcagttc ggtcggggaa taggtcgtgc cgcagacttc gcagttgtcg
     5161 ccgtattggt cttgggcgtg gcatttcggg cattcgcctt taacgaaacg gtcgggcagg
     5221 aacatttgtt tttcagggtc gaaaagctgc tcgatgacgc ggctttcgat tttgccgttg
     5281 gctttcagcg cgcggtaaat gtcttgggaa aactgtttgt tttcagggga atgggtgctg
     5341 taataattgt cgtagccgat gttgaaaccg gtaaagtcgg cgaggtgctc ttcgcgcact
     5401 ttggcaatca tgtcttcggg cgcgatgcct tgtttttgcg cggcaagcat cacgggcgtg
     5461 ccgtgggtgt cgtcggcgca gcagtagtag cattcgtggc cgcgcagttt ttggaagcgc
     5521 acccaaacgt cggtttggat gtgttcgacc atgtggccga ggtggatgct gccgttggca
     5581 tagggcaggg cggaggtaac taagattttg cgtgtcatat tgtgctttgc aaacaatggg
     5641 taaaggcgga ttataccgca aatcaaacgg ggaaatgccg tctgaagcct gaaaaatcgg
     5701 gcttcagacg gcattttgcc aatcggcggg atttattcga cggttacgga tttcgccaga
     5761 ttgcgcggct tgtccacatc cgtgccgcgt gcgagggcgg tgtggtaggc gaggagctgc
     5821 acggggatag tatgcacgat gggggacagt ttgccgacgt ggcgcggtgc gcggataacg
     5881 tgcacacctt cggtggcatt aaaattgctg tcgaggtcgg caaagacgaa aagttcgccg
     5941 ccgcgcgcgc cgacttcctg catattggct ttgactttgt ccaacaggct gtcgttgggt
     6001 gcgatgacga cgacgggcat gttttcgtcc accaaggcaa gcggcccgtg cttcagttcg
     6061 ccggcaggat aggcttcggc gtggatgtag gtgatttcct tcagttttaa cgcgccttcg
     6121 agggcaatcg ggtaatggat gccgcgcccc aaaaacagcg cgctggtttt cttggcaaac
     6181 tgttgcgccc aagcggcaat ttgcggttcg aggtttaaag cgtgctgcac gctgccggga
     6241 agctggcgga gttcttcggt ataacgtgct tcatcttcgc cggataccaa gccgcgtacc
     6301 ttcgccagcg ttaccgccaa accgaacagt gcgaccagct gcgtggtaaa tgctttggtc
     6361 gaggcgacgc cgatttccgc accggcacgg gtataaagca cgaggctgct ttcgcgcggc
     6421 agggcggatt ccatcacgtt gcaaatggag aggctgtggc ggtgtcccaa agatttggcg
     6481 tatttcaaag cctccatcgt gtccagcgtt tcgccggatt gggaaatggt aatgaccagt
     6541 tggtccggat cggcaatcac gctgcggtat cggtattcgc tggcgatttc gacgtcggtc
     6601 ggaattttgg cgatggattc gagccagtat ttggcggtca gcgcggcgta ataggacgtg
     6661 ccgcaggcaa ggattttgac gctgcggatg ctttcaaaca cgcttttggc gtttttgccg
     6721 aagttttcgg ggatgaagcc gccgtcgagg aaaacctccg ccgtgtctgc aatcgcgcgg
     6781 ggctgctcgt ggatttcctt ttgcataaag tggctgtacg gccccagctc caaagaggcg
     6841 agcgagagtt cggatacctt gactttgcgt tcggcaggca ggccgttttt atcggtcagc
     6901 cttttgatgc cgtctgaagc cagcagtgcg atgtcgccgt cttcaaggta cgccacgcgg
     6961 cgcgtaaagg cgatgacggc ggacacgtcc gaagcgataa aggtttcatc gtcgcccaaa
     7021 gcgaccaaaa gcgggcagcc catacgcgcc acaactaatt catcaggctt gtcttgggca
     7081 ataaccgcga tggcgtatgc gccgtggaaa cgtttgaccg cttcctgcac ggcttcaaac
     7141 agcctgccgc cgttttgcgc gtattcgtga ttgatgctgt gggcgatgac ttcggtatcc
     7201 gtttgcgatt caaaccggta tcccaaacct tccaagcgtt tgcgttcgct ttcaaagttt
     7261 tcgatgatgc cgttgtggac gacggctatc atcccaccgg aaatatgcgg atgggcattc
     7321 ggctcggtaa cgccgccgtg tgtcgcccaa cgcgtatgcc cgatgccgat gccgccgctg
     7381 atgccttttt cgcgtgctgc gtcttccata agctgcacgc gtccgacgcg gcgcacgcgt
     7441 ttgattttgc cgtcggtgtt gacggcaatg cctgatgagt cataaccccg gtattcgagg
     7501 cgtttgaggc cgtcggtcag aaaatcgacg acgttgtgat gggcgcggat ggcgccgacg
     7561 ataccgcaca taactgttcc ttagtatccg gttgaaaaaa aacaggcgcg gacggctccc
     7621 gtgccgcacc ttcctcttcg gattataaac cgcctcccgc gccgaaaaac agcaaaatgc
     7681 cgtctgaagg cttgggcttg ctcaaaaaag ggagggattt ccccgcctga ccgggatggg
     7741 cgttcagacg gcattgcctg ccgccggttt atagttttta caaatcaata ttgacaagct
     7801 gaaaaaaaaa aacaatatac tcgctcggtc ttaatgttaa cggagtatgg aaatgaaaca
     7861 aatgctttta gccgtcggcg tggtggcggt gttggcgggc tgcggcaagg atgccggcgg
     7921 ttacgagggt tattggcgcg aaaaatcgga caaaaaagag ggtatgattg ccgtcaaaaa
     7981 agaaaaaggc aattacttcc ttaataaaat caacgtgttt acaggtaagg aagaatctat
     8041 gcttttgtct gaaaaagacg gcgcgctttc gataaacacg gggatagggg aaatcccgat
     8101 caaactttcc gatgacggga aagagctgta tgtcgaacgc aggcagtatg tcaaaaccga
     8161 tgcggcgatg aaggacaaaa tcatcgccca tcagaaaaag tgcggacaaa cggcacaggc
     8221 atatctcgac gcgcgaaatg cgttgccgtc aaaccagacg tatcagcagc atcaggcggc
     8281 gatcgagcag ttgaaacggc ggtttgaagc cgagtttgac gaattggaaa aagaaatcaa
     8341 atgcaacggc aaaccgacat tgttgtttta gcgtaggaca accgggagga tgccgccgtc
     8401 cgaatcggac gtgcggtttc tgtaccggta cgggcgggca ggaatgtccg ctttttttgt
     8461 tcggacgcgt ttgaataccc gtttgattcc gtccgtttgc aaagggtatt tttgttcggg
     8521 cggaaattat agtggattaa ca
//
LOCUS       DAXHTR010000091         8516 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.091, whole genome shotgun sequence.
ACCESSION   DAXHTR010000091 DAXHTR010000000
VERSION     DAXHTR010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.091"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="ACFNML_001429"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="ACFNML_001429"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFC6392793.1"
                     /translation="MESENIISAADKARILAEALPYIRRFSGSVAVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGEFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRVPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1160..2290
                     /locus_tag="ACFNML_001430"
     CDS             1160..2290
                     /locus_tag="ACFNML_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HFC6392794.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDHQDATARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTLEKSPEVFFVREKMLETGVDLFLDIHGDEGLPFIFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANMTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNIIGDLR"
     gene            2361..3212
                     /gene="lgt"
                     /locus_tag="ACFNML_001431"
     CDS             2361..3212
                     /gene="lgt"
                     /locus_tag="ACFNML_001431"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFC6392795.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDLEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3295..3957)
                     /locus_tag="ACFNML_001432"
     CDS             complement(3295..3957)
                     /locus_tag="ACFNML_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HFC6392796.1"
                     /translation="MDSRKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEDNGTIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4052..5845)
                     /locus_tag="ACFNML_001433"
     CDS             complement(4052..5845)
                     /locus_tag="ACFNML_001433"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFC6392797.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRSLDLNAFLQKNESLERTEMTQTNRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6091..7200
                     /gene="proB"
                     /locus_tag="ACFNML_001434"
     CDS             6091..7200
                     /gene="proB"
                     /locus_tag="ACFNML_001434"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFC6392798.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGSALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVPVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     GVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7213..8475
                     /locus_tag="ACFNML_001435"
     CDS             7213..8475
                     /locus_tag="ACFNML_001435"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFC6392799.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALFAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVAALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KTGLAQTRIDPDALQLIEDTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYVDKDADLEKAIRIVRNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIETHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1909 a         2425 c         2227 g         1955 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccgg acaaacgggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaccctgcc
      181 gtcgatgatg tgcgtggctt tcacgccgtt gacggcggct tcgaccgcgg aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 aagtttttcg gcgttcaatt cttccgccaa tttgcctgcc accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcaatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctt acgccgaccg cgtgtccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacatgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaactcac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgccg tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatcgcg
      841 ggcaaaccct tctttcaagg caggttcggt catcgcgttg ccgccgtatt tgatgacggc
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagcgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt cagactccat aacggctccc tattgttaac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca gactttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gactttccca tgattaaaat cagcacccgc ttcgatgccg gctcggtcgt
     1201 cgtcaaagac ctgaccgatc cttccaacat ccgcctcgcc ctgcgtcccg acaacgcctc
     1261 cgattttgca caatggttct acttccggct gcaaggcgcg gcatatcaaa actgcatcat
     1321 gcattttgaa aacgcggcag aagccgccta cccgaaaggc tgggaaggct atcaggcgtg
     1381 tgcctcatac gaccgccgca actggttccg cgtaccgact tcctacgaaa acggcgtact
     1441 gaccgtcaac cataccccgc tgtccaatag cgtgtactac gcctatttcg aaccctattc
     1501 cgaagaacag cacctcaacc tcctcggcga cgcgcaaggc agcggcctgt gccgcatcga
     1561 cgacttgggc agcaccgtgc aagggcgcga catcaatctg ctgaccatag gcaatcaggt
     1621 cgaaagcgat ttgaaaatct ggattaccgc ccgccagcat ccaggcgaaa ccatggcgga
     1681 atggtttgtc gaaggactgc tcggcaggct gctcgaccat caggatgcaa ccgcgcgcac
     1741 cctgctcgat cgcgccacgt tctatatcgt ccccaacatg aaccccgacg gttcggcact
     1801 gggcaacctt cgcaccaacg ccgccggtgc gaacctcaac cgcgaatggg aaaatccgac
     1861 tttggaaaaa agccccgaag tgttcttcgt gcgcgaaaaa atgctggaaa ccggcgtgga
     1921 tttgttcttg gacatccacg gcgacgaagg gctgcccttt atctttgttg caggaactga
     1981 aggcgtgcca aactacaatc cgcgcatttc cgcgttggaa acacagttca aaaccgccct
     2041 tttgaacgcc agccccgatt tccaagacga atacggttac gaaaaagatg cgcccggtca
     2101 ggcaaacatg accttggcaa caaactgggt cggcaaccgt ttcaactgcc ttgcctatac
     2161 gttggaaatg ccctttaaag acaatgccaa cctgcccgac gacgatttcg gatggaacgg
     2221 tcaacggtcg ttgcgcttgg gcgaagccgc gttgtccgcc atcctcaaca tcatcggcga
     2281 tttgcgctga aacccttatt gcagaaatgc cgtctgaagc atcatgcctt tcagacggca
     2341 tttgaaaacg aaagcctgtt atgattatcc atccccaatt cgatcccgtc ctcatcagta
     2401 tcggcccgct tgccgtccgc tggtatgccc taagctacat cctcggattt attcttttta
     2461 cctttctcgg cagaaggcgc atcgcgcaag gcttgtccgt ttttaccaaa gaatcgcttg
     2521 acgacttcct cacttggggc attttgggcg taatttttgg tgggcgtttg ggttacgtcc
     2581 tgttttacaa gttttccgac tacctcgccc atccgcttga tattttcaag gtatgggaag
     2641 gcggaatgtc gttccacggc ggctttttgg gtgtagttat tgccatatgg ttgttcggcc
     2701 gcaaacacgg catcggcttc ctcaaactga tggacacggt cgcaccgctc gttccgctgg
     2761 gtctcgcttc gggacgtatc ggcaacttca tcaacggcga actttgggga cgcgttaccg
     2821 acatcaacgc attttgggca atgggcttcc cgcaggcgcg ttacgaagac ctcgaagccg
     2881 ccgcgcacaa tccgctttgg gcagaatggc tgcaacaata cggtatgctg ccgcgtcatc
     2941 cctcgcagct ttatcagttt gcacttgaag gcatctgcct gttcgccgtc gtttggctgt
     3001 tctctaaaaa acagcggccg accggacaag tcgcctcgct cttcctcggc ggctacggca
     3061 tattccgctt cattgccgaa tttgcacgcc aacccgacga ctatctcggg ctgctgacct
     3121 tggggctgtc gatggggcaa tggttgagcg tcccgatgat tgttttgggt atcgtcggct
     3181 ttgtccggtt cggcatgaaa aaacagcact gaccgcctgt gaaaaatacc gtctgaaaca
     3241 tggaaatccg gtttcagacg gcatttttac gtttggttca tggaaacaaa cgcttcaatt
     3301 cttaccgcaa cacgccttgt attttctgcc gctgccgcac ggacagggat cgttcctgcc
     3361 tgttttttcg ccttccctgc ggacggtttg cggcttgttg atgaccgcct gccagtagcg
     3421 gtagatatcc gccaatgcat aaggcaactc ggattccagt tccgccagct cgccttctgt
     3481 gaattgcaga cggatagtgc cgttgtcctc ttcgtcgtaa ataccgccca atgccatgat
     3541 gggataaaac aactcttcaa acgcttcatc atcgacggct tcaaaccaat cggtcggcac
     3601 aatatccaaa ccgtaaagat aagcattgca ccatgtgtaa aaatcgctgc cgccgtcttc
     3661 gttttcatac agccacaaat cgggcagttt tttatccgac atcgcggcgg ttgtttccat
     3721 cgccattgcc aaaaccagcc gttcgatttc ggaacgttcg gcggcggtaa attgcgattc
     3781 gtcgcccaac acttcgggca gccagtcgag cggtgtcaat ttgtccggcc cgctcaacag
     3841 cgccgtcata aaaccttgaa cctcgtcgca acgcatcgtg ttgccttgtt cgcttttggc
     3901 atccaacaat tcgctcaacc gccgtttgga tgcttcggta aattttcggg aatccatcat
     3961 tttccttttc aaatgggttt tgcgccctat tatcgccgca atgccgtctg aagccttttt
     4021 atgcttcaga cggcatcgtc tgttttcacg ctcaaatcgt accgctgcct tgcgctttcg
     4081 gtttggcggc actaaattcc agtttgctca aagcggaaag gtaggctttg gcggtggcga
     4141 ccaaaacgtc ggtatccgcg ccctgaccgt tgacgacgcg gttgccgcgc gccagacgga
     4201 cgctggtttc gccctggctt tccgtacctt gcgtgacggc gttgacggaa taaatctgca
     4261 aagccgcgcc gctttgcgcc acgctttcaa tcgctttgaa aatcgcatcc acggggccgg
     4321 agcctgtggc ggaagcgcgt ttttcttcgc ctttgatgct gaacacgatg tcggcgcgcg
     4381 gctcttcgcc ggtttcggtg ctgatttttt gggagatgaa tttgtagctc tcggcattca
     4441 tgctgcccat ttcgtcggat accagtgcgt gcaggtcttc atcgaagatt tcgcgttttt
     4501 tgtcggcgag ttctttgaag cgggcaaacg cggcgttcag tgcctcttcg ctttccaact
     4561 cgatgcccaa atccgccagc ttggttttga aggcgttgcg gccggacaat ttgcccaagc
     4621 tcaaacggtt tgttgcccag ccgaccgatt cggcggacat aatctcgtaa gtttcgcggt
     4681 gtttcagcac cccatcctga tggatgcccg attcatgcga aaaggcattg gcaccgacaa
     4741 tggctttgtt gggctgcacg ggatagcccg taatggtgga caccagtttg gacgaaggca
     4801 cgatttgcgt ggtatcgatg ccggtttcca agccgaacaa gtcgtggcgc actttcaacg
     4861 ccatcacgat ttcttcaacc gaagcattgc ctgcacgttc gcccaagccg ttgacagtac
     4921 attccacctg acgcgcgccg ccttttaatg cggcaagcga attggcaacc gccaagccca
     4981 aatcgttgtg gcagtgtgcc gaccaaacga ctttgccgcc gttgggcgtt ttggcaatca
     5041 gttcgcggaa aaattcttcg gttttgtacg ggattgaata gccgacggta tcgggaatat
     5101 tgatggtggt cgcgcccgct tcaatcaccg cgccgcagat ttcggcaagg aaatcgattt
     5161 ccgaacgcaa cgcgtcttcg caggaaaatt ccacatcgtc ggtgtattca cgagcgattt
     5221 tcaccgcttt gaccgccgcc tcaatcacct gcttcggctt catcttcaat ttgtactcca
     5281 tatggatggg gctggtggcg atgaaggtgt ggatgcgttt tttcggcgcg ggcgcaacgg
     5341 cctcacccgc ctgacggatg tcccgctcga tggcgcggga caatgaacag accgttgatt
     5401 tggtaatggt tttcgcaatc gcattgaccg cctcgaaatc gcccgggctg gcagcggcaa
     5461 aacccgcttc gatgatgtcc acacccaatt tttccagctg gcgggcgacg cggatttttt
     5521 cctctttggt catagcggcg ccgggcgact gctcgccgtc gcgcagggtg gtatcaaaaa
     5581 taattacgcg gttggtctgt gtcatttctg ttctctccag agattcgttt ttttgtaaaa
     5641 aagcattcaa atccagcgac ctgccttgcg cttcagccaa cgcggtcagc ttttgcagtt
     5701 gcgccagggg cagcgacccg cgcgtgcgcc atttatagat ggcggcggtc gtcgcggcat
     5761 tttcgggatc gtgctgtttc aacgcttcgg caagcgcgtt cacgccgccg aaataagcaa
     5821 ccaagcggtc tatatctaat tgcatggtag tccttgtcta aaataaatca gcctacagca
     5881 gaaatatgga caggattata cgcattttgg acaaaacggc aatataaatt tgaaaaatct
     5941 caataagccg ccatagattt tattaatttt atacattttg tctattttat cgcagacgcg
     6001 gcgtgattgt tgacaattgc ggcagaacag ggcaaattcg gcggcacaag gcggcaaacg
     6061 agacaggaca aaccaacaag gacggaaaca atgaaataca aaagaatcgt atttaaagtc
     6121 ggcacatctt cgattaccca ttcggacggc agtctctcgc gcggcaaaat ccaaaccatc
     6181 acccgccagc ttgccgcatt gcatcatgcg ggacacgagc tggtcttggt gtcttccggc
     6241 gcggttgcgg cagggttcgg tgcgctgggt ttcaaaaaac gtccggtcaa aatcgccgac
     6301 aaacaggctt ccgccgccgt cgggcagggg ctgctgatgg aagaatatac ggcaaacctg
     6361 tcttcagacg gcatcgtgtc cgcacaaatc ctgctcagcc gcgccgactt tgccgacaaa
     6421 cgccgctacc aaaatgccgg cagcgcactt tccgtgctgc tgcaacgccg cgccgtcccc
     6481 atcatcaatg aaaacgatac ggtttcggtt gaggaattga aaatcggcga caacgacaca
     6541 ttgagtgcgc aagtggcggc gatgatacag gcagacctct tggtgctgct gaccgacata
     6601 gacggtcttt acacgggcaa cccgaacagc aatcccgatg ccgtacggct ggacaaaatc
     6661 gaacacatca accatgaaat catcgaaatg gcgggcggct cgggttcggc aaacggcaca
     6721 ggcggtatgc tgactaaaat caaagcggcg acgattgcga ccgagtccgg cgtaccggtc
     6781 tatatctgtt cctcgctcaa acccgatgca cttgccgaag cggcagataa tcaggcggac
     6841 ggctcgtttt tcgtcccccg tgccaaaggt ttgcggacgc agaagcaatg gctggcgttc
     6901 tattccgaaa gccggggcgg cgtttatgtg gacgaaggtg cggaacacgc tttgtccgaa
     6961 cagggaaaaa gcctgctgat gtcgggcatt gccggaatcg aagggcattt ttcccgtatg
     7021 gacaccgtaa ccgtgtacag caaggcaacc aaacagcccc tgggcaaagg gcgcgtcctg
     7081 ttcggttctg ccgccgccga agacctgctc aaatcgcgta aggcgaaagg cgtgttcatc
     7141 catcgggacg actggatttc catcacgccc gaaatacgcc tgcttctgac cgaattttag
     7201 aaaaggaaac ccatgtcaaa cacacaaaaa cagcttgccc ttgccaaagc ggcaaaaaaa
     7261 tccgtcaaca cggcggatac ggaagaaaaa aaccgcgcgc tgtttgctat ggcggacagc
     7321 ttggaagcgg cggcggcaga tattttggcg gcaaaccgtc aagacttgga agccgcggca
     7381 ggcaacattc ccgaaagcat gaccgaccgc cttttgttgg acggcaaacg catttgcgcg
     7441 atggcggacg gcatcagggc ggttgccgcg ctgcccgacc ccgtgggcga aatactggaa
     7501 acctcgactt tgcccaacgg cttggaaatc gtcaaaaagc gcgtggcgat gggcgttatc
     7561 ggcattattt acgaaagccg cccgaacgtt acttccgatg cggcggcttt ggcactgaaa
     7621 agcggcagcg cggtcgtact ccgcagcggc aaagatgcat tccaatccgc acgcgccatc
     7681 gttgccgccc tgaaaacggg gttggcacaa acccgcatcg accccgacgc gctccagttg
     7741 attgaagaca ccgggcgcga gggcagttac gaaatgatga gggcgaaaga ttatctagac
     7801 ctgctgattc cgcgcggcgg ggcggggctg atacgggcgg tggttgaaaa tgccgtcgtg
     7861 ccggtcattg aaacgggaac gggcatcgtc cacatctacg tcgataagga tgcggactta
     7921 gaaaaggcaa tccgcattgt ccgcaacgcc aaaaccagcc gtccgtccgt gtgcaactcg
     7981 atggaagtgc tgctggtaca tgaagacatt gctgccgact tcctgcccaa gctcgaacgg
     8041 ctgttggttc gcgaccgtat agaagccgga ctgccgcccg tccgcttccg tttggatccg
     8101 caggcggcgc ggcatatcgg cggcgaagcg gcgggtgcag acgatttcga taccgagttt
     8161 ttagactaca tcctcgccgt gaaaaccgtc gcttcggtcg aagaggcggt cgggcacatc
     8221 gaaacccacg gcacgcacca ttcagacggc atcgttaccg aaaaccgcca cgctgcggac
     8281 tatttcacga cccatatcga ttctgccgcc gtgtatgtca acgcgtccac gcgctttacc
     8341 gacggtggcg aattcggctt gggttgcgaa atgggcatct ccacgcaaaa actccacgcc
     8401 cgaggcccga tgggcttgaa agagctaacg agttataaat acatcgtaca aggcacggga
     8461 caggtcaggg aataatccga attcaaacgt ctgatatagt ggattaactt taaacc
//
LOCUS       DAXHTR010000092         8432 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.092, whole genome shotgun sequence.
ACCESSION   DAXHTR010000092 DAXHTR010000000
VERSION     DAXHTR010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.092"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            148..1317
                     /gene="prpF"
                     /locus_tag="ACFNML_001436"
     CDS             148..1317
                     /gene="prpF"
                     /locus_tag="ACFNML_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HFC6392800.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFAIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDPADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTATKAVMSRSARVMMEGWVRVPEDCF"
     gene            1731..2930
                     /locus_tag="ACFNML_001437"
     CDS             1731..2930
                     /locus_tag="ACFNML_001437"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFC6392801.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSIVLSCLGERLTTPEAVI
                     TFSKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAIGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMDKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            3288..3908
                     /locus_tag="ACFNML_001438"
     CDS             3288..3908
                     /locus_tag="ACFNML_001438"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6392802.1"
                     /translation="MAQFFAVHPDNPQERLIKQAVEIVNQGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKEVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVVDMTDGTELVRQGCGDTAVFGL"
     gene            complement(4035..4478)
                     /locus_tag="ACFNML_001439"
     CDS             complement(4035..4478)
                     /locus_tag="ACFNML_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HFC6392803.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAVTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            4652..5020
                     /locus_tag="ACFNML_001440"
     CDS             4652..5020
                     /locus_tag="ACFNML_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HFC6392804.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFA
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAAEPFLPA"
     gene            5065..6219
                     /locus_tag="ACFNML_001441"
     CDS             5065..6219
                     /locus_tag="ACFNML_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HFC6392805.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTHELSGF
                     YWHAHEMIWGYAGLVVVAFLLTAVATWTGQPPTRGGVLVGLTIFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(6276..7148)
                     /locus_tag="ACFNML_001442"
     CDS             complement(6276..7148)
                     /locus_tag="ACFNML_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HFC6392806.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHRCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALIENLWRAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            7233..8045
                     /locus_tag="ACFNML_001443"
     CDS             7233..8045
                     /locus_tag="ACFNML_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HFC6392807.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSPEAGKVMNTLL
                     VYGRDGKRTGLYHKMHLFGFSGLGERYAEADTIRAGGEVPHLSAEGVPVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGVVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         1827 a         2326 c         2436 g         1843 t
ORIGIN      
        1 taataatctg ccgtatcaca acacagtagc cgtagattgg ggcgaacccc gacagtttgc
       61 ggaatcaaac ggctttggtc ggagtggcag cctaatccac tataaaaatc gtgggcagag
      121 cccacgctac ataaggagaa tctagaaatg ccgcaaatta aaattcccgc cgtttactac
      181 cgtggcggta catcaaaagg cgtgtttttc aaacgttccg acctgcccga ggcggcgcgg
      241 gaagcgggaa gcgcacgcga caaaatcctc ttgcgcgtac tcggcagccc ggatccctac
      301 ggcaagcaga tagacggttt gggcaacgcc agctcgtcca ccagcaaggc ggtgattttg
      361 gacaagtccg aacgcgccga tcacgatgtc gattaccttt tcgggcaagt ttccatcgac
      421 aaaccttttg tcgattggag tggcaactgc ggcaacctca ccgccgccgt gggcgcattt
      481 gccatcgagc aaggcttggt cgataaaggc aagattcctt cagacggcat ctgcacagtc
      541 aaaatctggc agaaaaacat cggcaaaacc attattgccc atgtaccgat gcaaaacggc
      601 gcagttttgg aaacaggcga ttttgagctc gacggcgtaa cgttcccggc agccgaagta
      661 caaatcgaat ttcttgatcc agccgacggc gaaggcagta tgttcccaac cggcaatttg
      721 gtcgatgaaa ttgatgtgcc gaatataggc cgtttgaaag ccacgctcat caacgcgggc
      781 attccgaccg ttttcctgaa tgccgccgac ttgggctaca cgggcaaaga gttgcaagac
      841 gacatcaaca acgatgccgc agctttggaa aaattcgaga aaatccgcgc ttacggtgcg
      901 ctgaaaatgg gtctaatcag cgacgtatcc gaagctgccg cccgcgcgca cacgccgaaa
      961 gtcgccttcg tcgcgcccgc cgccgattac accgcctcca gtggcaaaac cgtgaatgcc
     1021 gccgacatcg atttgctggt acgcgccctg agcatgggca aattgcacca cgcgatgatg
     1081 ggtaccgcct ctgttgccat tgcgaccgcc gccgccgtgc ccggtacgct ggtcaacctt
     1141 gccgcaggcg gcggaacgcg taaagaagtg cgcttcgggc atccttccgg cacattgcgc
     1201 gtcggtgcag ccgccgaatg tcaggacgga caatggacgg ccaccaaagc ggttatgagc
     1261 cgcagcgcac gcgtgatgat ggaaggttgg gtcagggtgc cggaagattg tttttaaatt
     1321 gacgtagcat gggtttgccc gcgagccata aacaggtcgt ctgaaaaaca agtaaacatc
     1381 aaatcactga ccattccttt cccttgccct gtggcggaag gcggcaaatc acaaggaaga
     1441 acacggaaac cccgataaaa gacagcttcc cgtattaccg tcattcccgc gcaggcggga
     1501 atctagacct gtcaatatgg aggattggta ggggaaaaca ggtttcgtga gttctacatt
     1561 ctggattccc gccacagcct gtcctcgcgt aggcggggac ggaataacga tagaaaatgc
     1621 ggcatacgct ttgcccaaag aggccgtctg aaacaccttg cgcctgatgt ctgccttttt
     1681 cagacgaccc cacaccaaaa aaacaaccac aaactacaag gagaaacatc atgtccgacc
     1741 aactcatcct cgttctgaac tgcggcagtt catcgctcaa aggcgccgtt atcgaccgca
     1801 aaagcggcag catcgtccta agctgcctcg gcgaacgcct gaccacgccc gaagccgtca
     1861 ttacgttcag caaagacggc aacaaacgcc aagttcccct gagcggccga aattgccacg
     1921 ccggcgcggt gggtatgctt ttgaacgaac tggaaaaaca cggtctgcac gaccgcatca
     1981 aagccatcgg ccaccgcatc gcccacggcg gcgaaaaata cagcgagtct gttttgatcg
     2041 accaggccgt aatggacgaa ctcaatgcct gcattccgct tgcgccgctg cacaaccccg
     2101 ccaacatcag cggcatcctc gccgcgcagg aacatttccc cggtctgccc aatgtcggcg
     2161 tgatggatac ttcgttccac caaaccatgc cggagcgtgc ctacacttat gccgtgccgc
     2221 gcgagttgcg taaaaaatac gctttccgcc gctacggttt ccacggcacc agtatgcgtt
     2281 acgttgcccc tgaagccgca cgcatcttgg gcaaacctct ggaagacatc cgcatgatta
     2341 ttgcccactt aggcaacggc gcatccatta ccgccatcaa aaacggcaaa tccgtcgata
     2401 ccagtatggg tttcacgccg atcgaaggtt tggtaatggg tacacgttgc ggcgacatcg
     2461 atccgggcgt atacagctat ctgacttccc acgccgggat ggatgttgcc caagtggatg
     2521 aaatgctgaa caaaaaatca ggtttgctcg gtatttccga actttccaac gactgccgca
     2581 ccctcgaaat cgccgccgac gaaggccacg aaggcgcgcg cctcgccctc gaagtcatga
     2641 cctaccgcct cgccaaatac atcgcttcga tggctgtggc ctgcggcggc gttgacgcac
     2701 tcgtgttcac cggcggtatt ggcgaaaact cacgtaatat ccgagctaaa accgtttcct
     2761 atcttgattt cttgggtctg cacatcgaca ctaaagccaa tatggacaaa cgctacggca
     2821 attcgggtat tatcagcccg accgattctt ctccggctgt tttggttgtc ccgaccaatg
     2881 aagaactgat gattgcctgc gacactgccg aacttgtcgg catcttgtag ccaaaaaagg
     2941 gacgagtccg cacaatgccg tctgaaaccc caaacgcccg attaggctga tgaggatttt
     3001 agacggcatt gttcattttt ttgttatctt gcatttttgt gcggacggtg gaatttcatc
     3061 ctgtaaacat aaatatttgt cggaaaacag aaaccctccg ccgccatttc tacgaaagca
     3121 ggaaaccagc aacgcaaagc gacagggatt tgttggaaat gaccgaaacc gaacgaaccg
     3181 gattcccgcc tgcgcgggaa tgacgggatt ttctgttttt gtggaaatga cgggattttg
     3241 aatttcgggc gtacaatacg gaaaacatga cgataaggac acaaaccatg gcacagtttt
     3301 tcgccgttca tcccgacaat ccccaagaac gcctcatcaa gcaggcggtt gaaatcgtca
     3361 atcaaggcgg cgtggtggtt tatccgaccg attcgtgtta cgccttgggt tgcaaactcg
     3421 gcgataaggc ggcgatggaa cgcatactct ccatccgcaa aatcgatttg aaacaccacc
     3481 tgaccctgat gtgcgccgat ttgagcgagt tgggcacgta cgccaaaatt gacaacgtac
     3541 agtttcgtca gcttaaagcc gccacgcccg ggccttatac ctttatcttg caggcgacga
     3601 aggaagtgcc ggcgcgcacg ctgcacccga aacgcaaaac catcgggctg cgtattcccg
     3661 ataatgccat tgcacaagcc ctgctggggg aattgggcga gccgctttta agctgcaccc
     3721 tgatgctgcc cgaagacggc gaaccattga ccgatcctta tgaaatccgc gagcgtttgg
     3781 aacacgccgt cgatttggtg attgacggcg gctggtgcgg aaccgagccg accaccgtcg
     3841 tcgatatgac cgacggcacg gaattggtgc gccaaggttg cggcgatacg gcggtgttcg
     3901 gtttgtaggg aaaccgatgc cgtctgaagc atcggctgtt cagacggcat tgcgcgcctt
     3961 gccggcggca gtccgaaatg ccggcgcgta tcgcgctcgg tcggaatatc cgtttgaaac
     4021 ggcattttga tgcattactg caccgcaatc ggaattctcg gttcgtagag caggtcgtag
     4081 gtcggcttgt tgagcaggtc ttggagcgtg aaaccgtcca gatacgtgaa aaatgacttc
     4141 atcgcgccgc cgagtatgcc cgtcagccgg caggacggtg taatcaggca ttcgttgttc
     4201 tcgcccatgc actcgaccag ctgcatcggt tcgaggtggc ggacaaccga gccgatgttg
     4261 atgcggtcgg gcggtgcggc aagccgcaga ccgccgcctt ttccgcgcac actgtggagg
     4321 aagccgcctt tgaccagcgc ggtaacgacc ttcatcagat ggcttttgga aatgccgtag
     4381 gttacggcga tggtactgat gttgaccagc gcatcgtcgt tgatggcagt gtagataagg
     4441 acgcgcagcc cgtagtccgt atgttgtgtc aaatacatga ttttctcggt atggatcgtt
     4501 attcttatcg gtacggttta agtttcacgg aaaatacctt aatggttgaa accctgtccg
     4561 tcggggcggt agaatgcagc ctgtctgcgg cggtatgccg tctgaaacat ccgcgctacc
     4621 gtttgataat ttgttattgt aactcaaaat catgaaaccg ttgaaacgac atcccgccct
     4681 tatcgggctt tcgcgtgacc accaccattc gctttccctg tgcgtgcgtc tgttgcggac
     4741 gccggaagaa aggcatcggg acgaactcga accgcatttt gccgaattgg aaacccattt
     4801 tcgcgaagaa gaagccaagt ttgccccaat ttggcagaat gtcgcccccg aattgaaaca
     4861 acgtttcgag aaagaccacg cccgactgcg gcagatgatg gcaagccccg aatacggtaa
     4921 cgcggcgtgg aataccgctt ttgccacaac cctgcgcgac cacgcgcgct ttgaagaacg
     4981 cgagctgttt cccgccgccg aaccgttttt gccggcatga ttccgttttg cggcaaatat
     5041 attaatgata aacaaggaac acacatgaaa tttaccaagc accccgtctg ggcaatggcg
     5101 ttccgcccgt tttattcgct ggcggctctg tacggcgcat tgtccgtatt gctgtggggt
     5161 ttcggctaca cgggaacgca cgagctgtcc ggtttctatt ggcacgcgca tgagatgatt
     5221 tggggttatg ccggactggt cgtcgtcgcc ttcctgctga ccgccgtcgc cacttggacg
     5281 gggcagccgc ccacgcgggg cggcgttctg gtcggcttga ctatcttttg gctggctgcg
     5341 cggattgccg cctttatccc gggttggggt gcgtcggcaa gcggcatact cggtacgctg
     5401 tttttctggt acggcgcggt gtgcatggct ttgcccgtta tccgttcaca gaatcaacgc
     5461 aactatgttg ccgtgttcgc gctgttcgtc ttgggcggca cgcatgcggc gttccacgtc
     5521 cagctgcaca acggcaacct aggcggactc ttgagcggat tacagtcggg cttggtgatg
     5581 gtgtcgggtt ttatcggtct gattggtacg cggattattt cgttttttac gtccaaacgg
     5641 ttgaatgtgc cgcagattcc cagtccgaaa tgggtggcgc aggcttcgct gtggctgccc
     5701 atgctgaccg ccatgctgat ggcgcacggc gtgatgcctt ggctgtcggc ggctttcgcg
     5761 tttgcggcag gcgtgatctt taccgtgcag gtgtaccgct ggtggtataa acccgtgttg
     5821 aaagagccga tgctgtggat tctgtttgcc ggctatctgt ttaccggatt ggggctgatt
     5881 gcggtcggcg cgtcttattt caaacccgct ttcctcaatc tgggtgtgca tctgatcggg
     5941 gtcggcggta tcggcgtgct gactttgggc atgatggcgc gtaccgcgct cggtcatacg
     6001 ggcaatccga tttatccgcc gcccaaagcc gttcccgttg cgttttggct gatgatggcg
     6061 gcaaccgccg tccgtatggt tgccgtattt tcttccggca ctgcctacac gcacagcata
     6121 cgcacctctt cggttttgtt tgcactcgcg cttttggtgt atgcgtggaa gtatattcct
     6181 tggctgattc gtccgcgttc ggacggcagg cccggttgag acaaaccgcc gcagatttcg
     6241 ggtctgggcg gtttgctttt cagacggcag ggcggtcagt tgccgtccag ccagcggtcg
     6301 cgtgtggttt tggcttcttc aaaatagcgg tacagggctt cgcggtcgtc ggtggtcagg
     6361 atgtttgcca aaacgtccaa ctgtttgccc aagccttgaa ccagttgcag caggctgtct
     6421 ttgttggcaa ggcagatgtc cgcccacacg gcgggatgac cggaggcgat gcgggtgaag
     6481 tcccgaaagc ccgtggcggc gaatttcaga tattcctgtc cgtcggggtg gtcgagaatc
     6541 tggtggacat aggcgaaggc ggtcaggtgg ggcatatggg agacggcggc gaaaaccgcg
     6601 tcgtggcgtt gcgcgtccat cgtataaatt tccgccccga ccgcgcgcca caggttttct
     6661 atcaaggcaa tgccgtctga atgttcgccg ccgtgtggcg tgatgatgag ttttctgtgg
     6721 cggaacagcc cgaactgcgc ggcttgcgca ccgcttctgt ccgaaccggc aatcgggtgg
     6781 gcggcgatgc agcggtgcag gcggtcgggc agacagcggc ggaaggcttc gatgaccgaa
     6841 gatttggtgc tgccgacatc ggaaatccaa gtgtgttccg gcaaaacggg gcgcagcgcg
     6901 gtcaaaatgg cgggaacggt ggcgacgggc gtggcaatca gtaccaagtc cgcaccgccg
     6961 atgctgtccg cgtcgatggc aacggaagcc cggtcaatca cgccgcgttc caatgcacgt
     7021 tcgaggttgt cgcggtcggt gtcgataccg gtaacggtgc ggacgagtcc ctgccttttg
     7081 aggtcgagaa cgaacgaacc gccgatcagc cctacaccga tgagggcaat atggttcaaa
     7141 atgggcattt gtgtaaacgg ttttcgcaaa gtaccgtcat ggtagcctat cggcggaata
     7201 tgccgcaagg tcggcaggaa aaaggagaag aaatggacaa aatcagagtt gccgccgtgc
     7261 agatggtgtc gggcgtgtcg ccggaaacca acgtcgccgc catgaaacgc ctggtcgcac
     7321 gggcggcgga gcagggtgcg gattgggtgc tgctgcccga atattgggtg ctgatgggcg
     7381 caaacgatac cgacaaactc gcgcttgccg agcctttggg cggcggacgc tttcagacgg
     7441 cattgagcga aacggcgaaa gaatgcggcg tggtgctgtt tggcggaacc gtgccgctgc
     7501 aaagccccga ggcgggcaag gtcatgaata ctttattggt gtacgggcgc gacggcaaac
     7561 ggacggggct gtaccacaaa atgcacctct tcggcttttc cggtttgggc gaacgctatg
     7621 ccgaagccga taccatccgc gcgggcgggg aagtgccgca cttgtcggca gaaggcgtgc
     7681 cggtggcggc aggtatttgt tacgatgtcc gctttcccga atttttccgc cgccagctgc
     7741 cgtttgacgt attgatgctg cccgctgcgt ttacgcacac gacgggcaag gcgcattggg
     7801 agctgctgct gcgcgcgcgt gccgtcgaaa accaatgtta cgtcgtggcg gcggcacagg
     7861 gcggtttgca cgaaaacgga cggcgcacgt tcggacacag catgattgtc gatccgtggg
     7921 gcgacgtgtt ggacgtattg cccgaaggcg aaggcgttgt tacggcagac atcgatgcca
     7981 accgcctgaa cagcgtccgc aaccgcctgc ccgccttgaa ataccgggtt ttggatgccg
     8041 tctgaaggtt cagacggcat cggtgccggg gaatcagcgg gctgtaggat gggcttcagc
     8101 ctgccatttc catcaatccc acatttctac cgttttcatc gaatccatcg aatccgcccc
     8161 tttcgacgcc ccagtcctac gcaactgaac cgtcattccc acggaagtgg gaatctagaa
     8221 tctcgggggt ttcagtcatt tccgatagat tcccgccgcg tcggaggtct ggattcccgc
     8281 ctgtgcggga atgacgaatc catccatacg ggaacctgca ccacgtcatt cccacgaaag
     8341 tgggaatcta gaatctcggg gtttcagtca tttccgatag attcccgccg cgtcaggggt
     8401 ctagattccc gcctgtgcgg gaatgacgaa tt
//
LOCUS       DAXHTR010000093         8325 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.093, whole genome shotgun sequence.
ACCESSION   DAXHTR010000093 DAXHTR010000000
VERSION     DAXHTR010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8325
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.093"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(151..1083)
                     /gene="cysK"
                     /locus_tag="ACFNML_001444"
     CDS             complement(151..1083)
                     /gene="cysK"
                     /locus_tag="ACFNML_001444"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFC6392808.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVSNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1277..1882)
                     /locus_tag="ACFNML_001445"
     CDS             complement(1277..1882)
                     /locus_tag="ACFNML_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392809.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIMLWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMAVAYVH
                     RCGIDRQPPSTFGGSQLRLGGLTAALMQVSVLVLLLSEIGR"
     gene            complement(1879..2730)
                     /gene="dapF"
                     /locus_tag="ACFNML_001446"
     CDS             complement(1879..2730)
                     /gene="dapF"
                     /locus_tag="ACFNML_001446"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFC6392810.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFIPEHAPIAAWADRFRGIG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     AKGIIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAQVRETGSLIEPHRQFPERVNVGFMQIVSRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2868..3164
                     /locus_tag="ACFNML_001447"
     CDS             2868..3164
                     /locus_tag="ACFNML_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392811.1"
                     /translation="MFRILLLLFPCAILVACTRNIPANPTVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3178..3846
                     /locus_tag="ACFNML_001448"
     CDS             3178..3846
                     /locus_tag="ACFNML_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFC6392812.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3919..6039)
                     /gene="pnp"
                     /locus_tag="ACFNML_001449"
     CDS             complement(3919..6039)
                     /gene="pnp"
                     /locus_tag="ACFNML_001449"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFC6392813.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6276..7139)
                     /locus_tag="ACFNML_001450"
     CDS             complement(6276..7139)
                     /locus_tag="ACFNML_001450"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFC6392814.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYEVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7338..8213
                     /gene="rfbD"
                     /locus_tag="ACFNML_001451"
     CDS             7338..8213
                     /gene="rfbD"
                     /locus_tag="ACFNML_001451"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HFC6392815.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLAAAIIRLLQQTSPVRGIYHYAGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFGIKPSDWQ
                     KALAQVVSKLLSSGY"
BASE COUNT         2020 a         2380 c         1924 g         2001 t
ORIGIN      
        1 aaatatttcc ctcaagcaat gcggcggcac caatgcctga ttaaaacaca accggcgttt
       61 tcggcatata tgcaaaaacg cattttccgg cacggcgtta caccgatacc gaaaaatgcg
      121 tctgaatgtt tcgacaatcc gattaaagca ttatgccaaa tctgcaaaaa gtggcgtaga
      181 gagatagcgt tcgccataag aaggcagcag cacgactatc agcttgcctt cgttttcagg
      241 ctgtttggca agctgcaacg cactccaaac cgccgcaccg gaagaaatac ccaccaaaat
      301 gccttctttt tccgctattg cgcgggcggt ttcaaaagcc gcttcgttcg acactttggt
      361 aatgctgtcg taaattttgg tattcaaaac ggtcggaata aagcctgcgc cgatgccttg
      421 aatcgggtgc gggccttttt cgccgccgct caatacgggt gaagcttcag gctcgacggc
      481 aaccacttta acttcgggtt tgtatttttt caacacttcg cccacgccgg taatcgtacc
      541 gcccgtgccg acgccggcaa cgaagacatc gactttgccg tccgtatccc gccaaatttc
      601 ctcggcggtt gttttgcggt ggacttcggg gtttgcctca ttgtcgaact ggcgcggcat
      661 aaaataagtg tcgggatgcg cgtccaccaa ggatttcgct ttggcaatcg cgcccgccat
      721 accttcggcg gcaggggtca gaatcagctc cgcaccaaac gcgcgcaaca gcattttgcg
      781 ctccttactc atgctttccg gcatggtaat cgccagcttg tagccgcgtg cggcacatac
      841 cattgccaaa ccgacacccg tattgccgct ggttgcttcg acaatgacgg tgtttttgtt
      901 gattttgccc gctttttcgg caccctcgat cattgcttcg gcaatgcggt ctttgacgct
      961 gctgcctgga ttgaaaaatt ccagtttcac ggcaacctcc gccttcaaac cttcggtcag
     1021 acggttcagt ttgaccaaag gcgtgttgcc gattagttcg gtgatgctgt ttgcaatttt
     1081 catcttctgc tcctgaataa taaggtttcg acagtaaggc gcaatcccca taggcatcct
     1141 gtccgattgc aggggatttg cgccgaaaca taaacaaatt tcggatatgg ctttatccta
     1201 aacctatgcc gcctaaaaaa ccaatatcca aaatttcttt ctttataact ttttgttccg
     1261 atacggccgc accgtattat cttccaattt ctgaaagcag cagcaccagt accgagacct
     1321 gcatcaacgc tgccgtcaac ccgccgagtc gcagctgcga gccgccgaac gttgacggcg
     1381 gctgccggtc tataccgcag cggtggacat aggcaaccgc catcagcaca aactgcaaca
     1441 cgaaccaata aaccggcttc agcgcataaa gtttgagcag tgccgcaaac acaaacactt
     1501 gaaccgttcc cgaaaagcaa taatgcacca acgcacatat tccgacaaaa gcaagcgtca
     1561 tcgatacatt actgacggca aaaagcccga gcagcggatg ttcggggtct gcctgccatc
     1621 cgtttccatc cgttttccgg ttccaatgcc cgatgaaaaa aaatatgctg cccaaagtca
     1681 ggtaaaaatg ggggatgaac aagggcgcgg cgcgggtcat tccccatatg ccgggcatca
     1741 gctttgcccc caaaaccgat atgcccagcc acagcataat actcgcccaa aaccattgca
     1801 acattcctgc gcggaatatc agaaacagaa aaaccgccgc atacagtaag gcagacaaaa
     1861 aatccaaatg cagcaaaatc atgaatacgc caactcgcct tcaaacaccg tttccgcagg
     1921 gccggtcatc atcacatcgc cgccgcaggc ccattcgata tataaagttc cgcccggcaa
     1981 aatcacctct accgttttcc cttcatccaa caaccccaga cggatacccg ccaccacagc
     2041 cgcacacgcg cccgtaccgc acgcttgggt ttcgcccacg ccgcgctcga acacgcgcaa
     2101 acgaatcgcg gttcggctga caatctgcat aaagccgacg ttgacgcgtt cgggaaactg
     2161 cctgtgcggt tcgataagcg aaccggtttc gcgcacctgc gcgcattcta cgtcatcgac
     2221 cacaatcacc gcatgggggt tgcccatatt gacgcagctg acaggctgaa tgccggattc
     2281 gagatgcacc ccgtaaatac aggcatcatc cccctcgccc gattcgggga caaacggtat
     2341 ttcagacggc ataaacttcg gtttgcccat attgaccgta accatgccgt tatcggacaa
     2401 tttcggaaaa ataatgccct tggccgtttc aacacaaatt tctttcttat cggtcaaacc
     2461 cttgtctgca acaaaacggg caaaacaacg cgctccgttg ccgcattgcc cgacctcgct
     2521 gccgtcggca ttgaaaatac ggtaacggaa atccacacct tcggtttccg aacgcccgac
     2581 caccaaaagc tggtcgaacc cgatgccgcg gaaacggtca gcccatgctg caatcggtgc
     2641 atgctcagga ataaaatcct gactgaccgc gtcaatcacc ataaaatcgt tgcccaaacc
     2701 gtgcatttta gtaaatttca aagtgttcat attattctga aatcccgtat tatctgttgc
     2761 ttccgaattc cgaaatcccc cgcaccgaag ggaaatgctt ccatctcccg ccgcctgagg
     2821 attaaaatca gccattatac tgtttgttac cccttacaag aaacaccatg ttccgcatat
     2881 tactgttgct tttcccttgt gcaatccttg tcgcctgcac ccgaaacata ccggcaaacc
     2941 cgaccgtttc aaattgcgcg catcttcaaa accgcaccat cctgtatttt gacgcacacg
     3001 gcaaacccgc cgacggcgca gtaaagggcg gattttacaa aattatcgaa atacagggtg
     3061 caaactgcat ccttattcaa gactttaaca gcacgggcaa tgccctcggc ggatcctatg
     3121 tgccggactg ccgcaaatca tcccttttcc cgaaaaatat ttgacttcaa actgcctatg
     3181 aaccacgaca tcactttcct caccctgttc ctgctcggct tcttcggcgg aacgcactgc
     3241 atcggcatgt gcggcggatt aagcagcgcg tttgcgctcc aactcccccc gcatatcaac
     3301 cgcttttggc tgatcctgct gcttaacaca ggacgggtaa gcagctatac ggcaatcggc
     3361 ctgatactcg gattaatcgg acaggtcggc gtttcactcg accaaacccg cgtcctgcag
     3421 aatattttat acacggccgc caacctcctg ctgctctttt taggcttata cttgagcggt
     3481 atttcttcct tggcggcaaa aatcgagaaa atcggcaaac cgatatggcg gaacctgaac
     3541 ccgatactca accggctgtt acccataaaa tccatacccg cctgccttgc ggtcggaata
     3601 ttatggggct ggctgccgtg cggactggtt tacagcgcgt cgctttacgc gctgggaagc
     3661 ggtagtgcgg caacgggcgg gttatatatg cttgcctttg cactgggtac gctgcccaat
     3721 cttttggcaa tcggcatttt ttccctacaa ctgaaaaaaa tcatgcaaaa ccgatatatc
     3781 cgcctgtgta cgggattatc cgtatcatta tgggcattat ggaaacttgc cgtcctgtgg
     3841 ctgtaagccc cattcattcc cgatacccgt aaaaaatacc gtctgaaacc tgttcagacg
     3901 gcactttcac cctaagcgtt actcggcggc attttcctca cgggcaggcg cgtccagcag
     3961 ggctttgatg gacaggcgca cacggcctct gtcatccact tccaatgctt tcacgttcac
     4021 cacctgaccg acttgcaggt agtcaccgac attgcgtacg cgctcgtggg cgatttggct
     4081 gatgtgtacc aaaccgtctt tgcccggcat cacgctgaca atcgcgccga cgttgttgtc
     4141 gaggattttc accacagtgc cttcatacac tttgcccact tccacttcgg cagtaatctg
     4201 ctcgatacgt tttttcgccg catcgccggc ttcttgagtg gtcgcagcaa tggtaatcgt
     4261 accgtcttcg gcaatattga tttccgtacc ggtttcagcg gtaatcgaac ggatggtttc
     4321 gccgccctta ccgataactt cgcggatttt gtcttggttg attttcatcg taaacaaacg
     4381 cggcgcgtgt gcggacaact cttgcgggcc cgcaacggcg gctttcatct gatccaagat
     4441 gtgcagacgc gcttctttgg cctgtgccaa agcgatttgc ataatttctt tggtaatgcc
     4501 ttggattttg atgtccattt gcagcgcggt aacgccttcg gtcgtaccgg ccactttaaa
     4561 gtccatatcg cccaagtggt cttcgtcgcc caaaatgtcg gtcaggacgg caaatttgtt
     4621 gccttccaga atcagaccca tcgcgatacc ggcaacgtgt gctttcaaag gcacgccggc
     4681 agacagcagg ctcaggcagc cgccgcagac ggaagccata gaggaagagc cgttggattc
     4741 ggtaatttcg gagaccacgc gcatggtgta gctgaaatct tcaggtttcg gcaatacggc
     4801 caacaatgca cgtttagcca aacggccgtg accgatttca cggcgtttcg gcgcacctac
     4861 gcgacccact tctccggtag agtacggcgg aaagttgtag tgcagcatga agcggtcggt
     4921 gtattcgccg gacagcgcgt caatgatttg ctcgtcgcgc gaagtaccca aagttgcaac
     4981 cgccaaagct tgggtttcgc cacgggtaaa caatgcggaa ccgtgtgtac gcggcaatac
     5041 gccggtttgg atgttcaaag gacggacggt gcgggtgtcg cggccgtcga tgcgcggttg
     5101 accgtccaaa atttggctgc ggacaacatc ggcttccaag tgtttgaaaa tacctttgat
     5161 ttcgttggcg gccaaagtat cggtttcttc ggtaatcaag gcttctttta ccgcattcca
     5221 agcctcgtcc aatttggcgg aacgcgcttg tttttgacgg attttgaacg cttctttaat
     5281 ggtttcgccg gcaatcccgc ggactttggc aactaattct tcattggttt caggtgcttt
     5341 ccaatcccaa acttccggat tgacttcgtc ggcaaattca ttgatggcgt taatcgccgc
     5401 ctgcatttga tcgtggccgt aaaccaccgc gcccaacatc acgtcttcgg gcaggatttt
     5461 ggcttcggat tccaccatca acacggcttt ggacgtaccg gcgaccacca agtccaattg
     5521 cgatttagcc aattcggctt tagtcgggtt caaaacgtac acgccgttta tataacccac
     5581 gcgtgccgca ccaatcggac cggcaaacgg tacgccgctc aacaccagcg cggcagacgc
     5641 gcccaacatt gcaggaatgt cggaatcgat ttcgggatcg acggacacga ccatcgctac
     5701 gatttggatg tcgtggtaga aaccttcggg gaacagcgga cgaatcgggc ggtcgatcag
     5761 acggctggtc aggatttctt tttcgctttg tttgccttcg cgtttgaaga aaccgccggg
     5821 gattttacct gcggcgtaag tgcgttccaa ataatcgacg gtcaggggga agaagtcttg
     5881 accttctttc acttctttgt tggtggtaac ggcaaccaaa acaacggtgt cgcccataga
     5941 gactttaacg gcagcggcgg cttggcgggc aatttcgccg gtttccaaag taacggtgtg
     6001 gttgccgtat tggaaagttt taacgtgttt gtcgaacatt attgttcctt tcaaaatacc
     6061 gcactgctaa aacactaata atgcacacta aaatccgaat gtgcatagtt agggtttcag
     6121 accgtgcggc aggttgtaaa caagctttca gacgacattt cggacgctga aagcaattgc
     6181 ggattataaa ggcaaccatc cttaaaatcc agtattgata caataaaaag gccgtctgaa
     6241 acatatctct ttcagacggc cttcaaactt aaaaatcaat cctgagtcag caaagtcaat
     6301 gcttcccgat acttctcgac agttttctga atcacatcgg caggcacttt cggcgcaggg
     6361 gcttttttgt tccaaccgct ttgttccagc cagtcgcgga cgaattgttt gtcaaaagac
     6421 ggcggattgg tgccgacttc atattgatcc gccggccaga agcggctcga atcgggagtt
     6481 aatacctcat ccatcagcgt cagcgtaccg tttgtatcca aaccgaattc aaatttggta
     6541 tcgcaaataa taataccgcg cgatttggca tattccgccg cttcggtgta aagccgaacc
     6601 gccttggcgc gcacttcttc cgccaattct ttgccgataa tgcgtccgca ttcttcaaag
     6661 ctgatgtttt catcgtgatc gccgactgcg gctttggtcg atggcgtaaa aatcacttca
     6721 ggcagttgtt gcgcttcctg cataccttcg ggcagccgaa taccgcaaac cgagccggtt
     6781 ttttgataat ctttccaacc gctgcctgcc agataaccgc gcacaatcgc ctctactttc
     6841 accggagtga gctttttagc cacgacggcg cgtttttcta aaactttggc ttcgttttca
     6901 ggcaaaacat cgtaaaccgt ttgaccggta aagtggttgg gcataatatg cgccagtttt
     6961 ttaaaccaaa aattggaaat ctgcgtcaga atctcccctt tgctcggaat cgggtcgtcc
     7021 aaaatcacat caaacgcgga caggcggtcg gaagcgacca tcagcatacg tttatcgtcg
     7081 atttcatata aatcgcgcac ttttccagaa tagatcttta ccaaaccaat ctcactcatt
     7141 tcgccccccc tgaaaatatc ttgaaaatac cgacccgaca cccgacaggt ttgaatcgca
     7201 aaccgatatt ctagccgaag tcggcgcaaa acaataccca tggcacaaaa agccaacccg
     7261 tcaaccgtcg gcaaagtttt ggcactataa taccgacagc aagtcctaca atacactttt
     7321 accaaaggaa atacctcatg agaatcctat tgacaggctc gaaaagccaa ctggcacgct
     7381 gcctgcgcga ccgtcttccg gaagactggg aaaccattgc gacggattcc gcatccctag
     7441 acattaccga tgccgatgcc gtctgcaaca tggtcaaaag tttccaaccc gacgccattg
     7501 tcaacacggc tgcctatact gccgtcgaca aagcggaagg cgatgcggca gcggcatttg
     7561 ccgtcaatgc ttccgccgtt tacaaccttg ccttggcagc acatcgcgcc catgcccgat
     7621 tcatccacat ctcaaccgac tatatctttg acggtaaagg gaaaaggccc tatcaggaaa
     7681 gcgactttac caatccttcc aatgtatacg gacaatccaa aaccgccggc gaactgctcg
     7741 cactgtctgc caatcccgac agccttatcc tgcggacttc ttggctgttt agcgaatacg
     7801 gggacaactt tgtccgcacc atgctgaacc ttgcgcggga acgttccccg ctgtccgccg
     7861 tccacaacca aatcggctgc ccgacctatg ccggcgacct ggctgccgcc atcatccgcc
     7921 ttttgcagca aacgtcgccc gtgcgcggca tttaccacta cgccggcggc aaatccgtat
     7981 cctggtacga atttgcccaa catattttcc aagcggcatc gcaacagcag acatccttcc
     8041 ccgttcccga attgactgcc gtttcagaca aggaatatcc gaccgccgcc cccaggcccg
     8101 catacagcat tttggactgc cgcaaaatcg aaaacgactt cggcatcaaa ccgtcagact
     8161 ggcagaaagc ccttgcacag gtcgtttcca agctgctctc tagtgggtac tagatataat
     8221 gcagcatccg ataaagtaaa gccccgaagt ggcagcttcg gggcttatcg gatagaaagg
     8281 aggcttgaag aaatggcgaa agttatttta ttcctgtttc ttttg
//
LOCUS       DAXHTR010000094         8280 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.094, whole genome shotgun sequence.
ACCESSION   DAXHTR010000094 DAXHTR010000000
VERSION     DAXHTR010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8280
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.094"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(60..1505)
                     /gene="zwf"
                     /locus_tag="ACFNML_001452"
     CDS             complement(60..1505)
                     /gene="zwf"
                     /locus_tag="ACFNML_001452"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFC6392816.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWANNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            2021..3856
                     /gene="edd"
                     /locus_tag="ACFNML_001453"
     CDS             2021..3856
                     /gene="edd"
                     /locus_tag="ACFNML_001453"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFC6392817.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAVGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALMGGNIAKIRTGDLIRFDSVTGELNVLINEAE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            4037..4675
                     /locus_tag="ACFNML_001454"
     CDS             4037..4675
                     /locus_tag="ACFNML_001454"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016833 - oxo-acid-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFC6392818.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSHALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPDYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            4690..4908
                     /locus_tag="ACFNML_001455"
     CDS             4690..4908
                     /locus_tag="ACFNML_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392819.1"
                     /translation="MPSEALSSLRRHFAAWRRSRHTRQHHSDILPLKCPSAFRIIKIT
                     FSSFDSSRTAHTTRLKKCRLKRIQTASA"
     gene            complement(4932..5877)
                     /locus_tag="ACFNML_001456"
                     /pseudo
     CDS             complement(4932..5877)
                     /locus_tag="ACFNML_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            6040..7080
                     /gene="mutY"
                     /locus_tag="ACFNML_001457"
     CDS             6040..7080
                     /gene="mutY"
                     /locus_tag="ACFNML_001457"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFC6392820.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVRQFGG
                     TFPSERKDLETLCGVGRSTAAAISAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIVRNRDGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPAHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            7497..8057
                     /gene="sodC"
                     /locus_tag="ACFNML_001458"
     CDS             7497..8057
                     /gene="sodC"
                     /locus_tag="ACFNML_001458"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFC6392821.1"
                     /translation="MNMKTLLALAVSAVCSVGVAQAHEHNTIPKGASIEVKVQQLDPA
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         2089 a         2295 c         1961 g         1935 t
ORIGIN      
        1 tgctttactt gcgttcagac ggcatttcaa accccatgcc gtctgaacgc attattgtat
       61 tactgctctt cgtgccactt gtgtccgtcg cgcgccaata gttcgcgcgc ggcttcaggc
      121 ccccacgagt gtgcgccgta gccgtgcggc ggcgtggtgt tatttgccca gttttccaaa
      181 atcggcatca catattccca cgcggcttcg agttcgtcgc ggcggttgaa caaagcgagt
      241 ttgccgttaa tcacatccag cagcaggcgc tcgtaagctt ccgcgcggcg gccttccaat
      301 gctttgccca aatcggttgc cagcggcacg gtttcgacct tatttcctgc ccccggggtt
      361 ttcatctgcg tatagaggcg cacggattca tatggttgca actcgataac gagccggttg
      421 ggcgcggtgc gactgccttc aaaaatatgg ctgttcaaat ctttgaagtt caaaacgatt
      481 tccgccactt tgcccgccat gcgcttgccg gtacgcaggt agaagggaac gcccttccag
      541 cgttcgtttt cgatttcggc tttaatggcg acgtaggttt cggtaaagct gtcttgcgga
      601 acgttgattt cttcaagata gccgttcatg cctttggcgg cggtatattg tccgcgcacg
      661 acattttcat tgacagattc gacggtcagc ggcttcaatg acttgatgac tttgactttt
      721 tcatcgcgca ccgcgtcggc atccaagctg gcgggggctt ccatcgcagt catgcacagc
      781 atctgcatca aatggttttg caccatatcg cgcaacgcgc cggtaatgtc gtaaaactcg
      841 ccgcgctctt ccacaccgag ctgttcggcg atggtcaact gcacgctttc gatgtattta
      901 ttgttccaca gcggctcgaa cattacattg gcaaaacgca gcgcaagcag gttttgcagg
      961 ctttctttgc caaggtagtg gtcgatgcgg taaatttgcc cttctttgaa ataacgcgca
     1021 acatcggtat tgatttgctg ggaagaagcc aaatccgtac ccaacggttt ttccaaaacc
     1081 acacgcacgt tgtcggcgtt caaaccgatt ttggcaaggt tttcacatgt ttgcgcgaag
     1141 aatttcggcg cggtggaaag atagatgacg acattgtcgg tttctttgcg cgctttgacc
     1201 aaatcgccca aagcggcaaa atcgtccggc tgcgtaacat cgactttgag atatgcgaga
     1261 cgttcgacaa acgatgccca agcctcatcg gaaaaatttt ctttcacatg gattttggaa
     1321 ctggtttcca ccttcgccag aaaaccttcg gtatccaact cgctgcggct gacccctaaa
     1381 atacgccctt cgggatgaag cagaccggca acatgcgcct ggtacagaca gggcaacagc
     1441 ttgcgcatcg ccaaatcgcc ggtcgcaccg aacaacacca aatcaaaatt tgtttgtgta
     1501 ctcatcgtat tatctcgtca ggaaagaatt tttcgatgcc gtctgaaacc tgtttccccc
     1561 atcacgctgc atcgcaatat cggaaacaaa ggcaggcggc ataatgagta gtaatactac
     1621 acaccgctac actttttgtc tattcccatt tttacaattt atttgaccta gtccaaaaat
     1681 cgggcaggtt tcccctattc cgttacaaca atcggaagat tctgcgattt aaatcaaatt
     1741 tcttttcgat gtctgatttt tttgtaacaa aattacaaat tttgtactat aatagcaccc
     1801 gcttcccact ttcagacggc atacctttta aaatatagtg gattaacaaa aatcaggaca
     1861 aggcgacgaa gccgcagaca gtacagataa tacggcaagg cgaggcaacg ccgtactggt
     1921 ttttgttaat ccactatact caccgtctga atacccgata caaaaatcag aaacgcacaa
     1981 acaaatcccc aatacccccc gttccgacag gagaccgacc gtgaacacta ctcctatcca
     2041 ctccaaactc gccgaaatca ccgggcgcat tattgaacgc agccgtccga cgcgtgaaaa
     2101 atatctggcg aagatccgca gtgccaaaca aatgggacgc ttagagcgca accagctcgg
     2161 ttgctccaac ttggcgcacg gctatgctgc catgcctaaa agtatcaaaa tcgaaatgct
     2221 tcaggaaacc gtccccaact taggcatcat caccgcctac aacgacatgg tttccgcaca
     2281 ccagccgttt aaagacttcc ctgaccaaat caaagacgaa gcgcagaaaa acggcgcgac
     2341 cgcccaagtc gccggcggca cacccgccat gtgcgacggc atcacgcaag gctacgccgg
     2401 tatggaactg tcgctgttct cccgcgacgt gattgccatg agtaccgccg tcgggctgtc
     2461 gcatcaaatg tttgacggca gcctgtttat gggcgtatgc gacaaaatcg ttccgggttt
     2521 gatgataggc gcgctttcgt tcggtcatat tccgggtatc ttcgtccccg caggcccgat
     2581 gtccagcggt atcggcaaca aagaaaaagc ccgcacccgc cagcttttcg ccgaaggcaa
     2641 ggtcggacgc aacgaacttt tgaaaagcga aatgggttct taccacagcc cgggcacctg
     2701 cactttctac ggcacggcaa actccaacca aatgatgatg gaaatgatgg gcgtgcacct
     2761 gcctgccgcc gccttcgtcc acccttacac cgacctgcgc gaagccctaa cccgctacgc
     2821 cgccggacac ctcgcgcgcg gcatcaaaaa cggcacgatt aaacctttgg gcgaaatgtt
     2881 gaccgaaaaa tcctttatca acgccttgat tggcctgatg gcaaccggcg gttcgaccaa
     2941 ccacaccatg cacctcgttg ccatggcacg cgccgccggc gtgattttga actgggacga
     3001 cttcgacgaa atttcctcca tcatcccgct gctcattcgc gtttatccga acggcaaggc
     3061 cgacgtgaac cactttaccg cagcgggcgg actgcctttc gttatccgcg aattgctgaa
     3121 tgcaggcctg ttgcacgacg atgtcgatac cgtcgtcgga cacggtatgc gccactacac
     3181 caaagagcct ttcctcatcg acggcaaact cgaatggcgc gaagcccccg aaaccagcgg
     3241 caacgacgac atcctgcgta aagctgacaa cccgttctcc cccgacggcg gtctgcgcct
     3301 gatgaaaggc aacatcggac gtggcgtgat taaagtgtcc gccgtgcgcg aaggctgccg
     3361 cattattgaa gcgcctgcca tcgtgttcaa cgaccaacgc gaagtgttgg ctgcgtttga
     3421 acgcggcgag ttggaacgcg attttgtgtg cgtcgtccgc taccaaggcc cgcgtgccaa
     3481 cggtatgccc gaattgcaca aactgacccc gcctttaggc atcctgcaag accgcggctt
     3541 caaagtggcg ctgctgaccg acggccgtat gtccggcgcg tccggcaaag ttccagcctc
     3601 catccacatg acacccgaag ccctgatggg cggcaacatc gccaaaatcc gtaccggcga
     3661 cctgatccgc ttcgactccg ttaccggcga actcaacgtc ctgattaacg aagccgaatg
     3721 gaacgcccga gaagtcgaaa gcatcgactt gggcgcgaac caacaaggct gcggccgcga
     3781 acttttcgcc aacttccgca gcatgaccag cagcgccgaa accggcgcga tgagcttcgg
     3841 cggcgaattt gcctgatgcg cgtttcagac ggccttttca gaccgaaggc cgtctgaaaa
     3901 attattcaag tgttttaaga tagacgtagg tcggattctc gaatccgaca cagccgtcca
     3961 agatgtcggt ttcttgaatc cgacctacaa cctgtcccat cttaataaaa taccccatcc
     4021 cacccggaga accgaaatgt ccaaactgac cccccgcgaa atcctgaccg ccggcgcagt
     4081 tgtaccggta atggcgattg acgacttgag tactgccgtc gatttgtccc acgcccttgt
     4141 cgaaggcggc atccccaccc tcgaaatcac cctgcgcacc cctgtcggcc tcgatgccat
     4201 ccgcctgatt gccaaagaag tgcccaacgc catcgtcggc gcgggtacgg ttaccaaccc
     4261 tgaacagctc aaagccgtcg aagacgcagg cgcggttttc gccatcagcc cgggtttgca
     4321 cgaatccctc gccaaagccg gccacaacag cggcatcccc ctgattcccg gtgttgccac
     4381 cccgggcgaa atccaactgg ctttggaaca cggcatcgac accctcaaac tcttccctgc
     4441 cgaagtcgtt ggcggcaaag ccatgctcaa agccctgtac ggcccttacg ccgatgttcg
     4501 cttctgcccg acaggcggca tcagcctcgc caccgcgccc gattacttgg cactgcccaa
     4561 cgtcctgtgc gtcggcggct catggctgac gccgaaagaa gccgtgaaaa acaaagactg
     4621 ggacaccatc acccgtctcg ccaaagaagc ggcggcattg aaacccaaag cctgaaccgc
     4681 atcgtaaaaa tgccgtctga agccctttcc agtctcagac ggcattttgc cgcttggcgg
     4741 cgcagtcggc atacacggca gcaccactcc gacatactgc ccctaaaatg cccatccgcc
     4801 ttccgcataa taaaaataac gttcagttca ttcgacagca gccggacagc ccatactacg
     4861 cggctgaaaa aatgccgtct gaaacgcatt cagacggcat ccgcttaaaa aaacaactga
     4921 ttcaaggccg attaatccgc ttccaaaacc actttcatcg cttgattttc ggcggcgtgt
     4981 ttgaacacgt cgtaggcttt ttccaattcg ctgaatttga aacggtgggt caacattttg
     5041 gtgtaatcga cggagctgct ggaaattgcc ttcatcagca tttcggtggt attggcgttt
     5101 accaggccgg tagtgatggc aagcttttta atccacagtt tttccagttt gaaatcaacg
     5161 gattgaccgt gtacgccgac aacggcgata tggccgccgg gtttcacaat gtcttggcac
     5221 atattccaag ttgcagggat accgacggct tcgatcgcgc agtccacgcc gtcttcgccg
     5281 acgatagcga caacttgctt gcttacatcg ccggaagcag ggttaatggt atgggttgcg
     5341 cccaactctt tcgccagttt caaacggttt tcgtccatat cgcaaacaat gatggcggcg
     5401 gggctgtaca gttgggcggt cggcaaggcg tcgctcaaca acagggcgac ttcttcgttg
     5461 acgttgtcgg gcagcggcac gaggctgttg tcggcataag gcgtgcggac gtattctgcc
     5521 tgcgtgccgt caatcatgta gcccaaaatc cagccgccgt tgcggcaatg cgaataaagt
     5581 tggattttgc agttgtcgca agtacagcat ttgctgacgc atgaaataat gactttatcg
     5641 ccgactttga tgttttttac agcctcgccg acttcttcta caataccgat gccttcatga
     5701 ccgagaatac ggccgtcggc gacttcgggt ttttttgcct ttccaaatac ccaaatcggt
     5761 accgcaaatc gtggttttga cgattttcac caccgcatcg gtcggatcga taatctgcgg
     5821 acggggtttt tcttcaaaac ggatgtcgtt tgcgccgtga taaaccattg ctttcatgct
     5881 gatactcctt gcttgttgat aaataatttc aataccgcaa taaagtttcc ttatatgagt
     5941 tatatgcccc tacaaaaaat aagtcaataa gaattatttt cacaatgtta tacaataaca
     6001 tactatttta aatataaata aaaccaccga ttgatattaa tgaacacacc catccccttc
     6061 tccgaacggc tcatccgctg gcaaaaacaa cacggtcgcc accacctccc ttggcaggtc
     6121 aaaaaccctt attgcgtctg gctttccgaa atcatgctcc agcaaacgca agtcgccacc
     6181 gtgttggact actatccgcg cttcttagaa aaattcccga ccgttcagac gcttgccgcc
     6241 gcgccgcaag acgaagtgtt gtcgttgtgg gcgggcttgg gctattacag ccgcgcgcgc
     6301 aacctgcaca aagccgcgca acaagtcgtc aggcaattcg gcggcacgtt tccgtcggag
     6361 cgcaaagact tggaaaccct ctgcggcgta ggcagaagca ccgccgccgc catttctgcc
     6421 ttctccttca accgccgcga aaccattttg gacggcaacg tcaaacgcgt actctgccgc
     6481 gtgttcgccc gcgacggcaa tccgcaggac aaaaaatttg aaaactcgct ctggacactt
     6541 gccgaaagcc tgctgccgtc tgaaaacgcc gatatgcctg cctatacaca aggtttgatg
     6601 gatttgggcg cgaccgtgtg caaacggacg aaacccttgt gccaccaatg cccgatggcg
     6661 gacatctgcg aagcaaaaaa gcaaaaccgc accgccgagc tgccgcgcaa aaaaaccgcc
     6721 gccgaagtac cgaccctgcc gctttactgg ctgattgtcc gcaaccggga cggcgcgatt
     6781 ttgctggaaa aacgccccgc caaaggcatt tggggcgggc tgtattgcgt gccgtgtttt
     6841 gaaagtttga acgggctttc cgactttgcc gccaaattct ccctgaccat ggcagatatg
     6901 gacgaacaaa ccgccctgac ccaccgtctg acgcaccggc tgctgatgat tacgccgttt
     6961 gaaggacaaa tgccgtctga aagcccttca gacggcattt ggataaagcc ggcgcatttg
     7021 aaagattacg gcctgcccaa gcctttggaa atttatttaa acggtaatag gttagaataa
     7081 acaaaataaa cccattgaac tgttgtttgc aggtatcgca gcaagaacaa ccgatgaatt
     7141 tgggtcgtat tttaggcggt gggataatgt tcaaatggga catttggaac ggaagaagtc
     7201 ggcaatttaa aaaggattta aaaagcaaag aaggtcaaaa acatgaacac aaacttaaat
     7261 gacaaagaca aagccatgga taccgcaatc aggtttcaga aaaggatgag gattccgaaa
     7321 tttttctttt taattctctg aatcacaatg gttttggcat ttatccaaga cgtgataacg
     7381 ggttctaatt ttctgcaaat aacaattaat gtaaaatttt cgtaaaaatt tatcggcttt
     7441 taaaacaaaa ttgactaaaa tagtcgcgag tttttactgc aataaaggag attgcaatga
     7501 atatgaaaac cttattagca ctagcggtta gtgcagtatg ttcagttggt gttgcgcaag
     7561 cacacgagca taatacgata cctaaaggtg cttctattga agtgaaagtg caacaacttg
     7621 atccagcaaa cggtaacaaa gatgtgggta cagtgactat tactgaatct aactatggtc
     7681 ttgtgtttac ccctgattta caaggattaa gcgaaggctt acatggtttc cacatccatg
     7741 aaaacccaag ctgtgagcca aaagaaaaag aaggtaaatt gacagctggt ttaggcgcag
     7801 gcggtcactg ggatcctaaa ggtgcaaaac aacatggtta cccatggcaa gatgatgcac
     7861 acttaggtga tttacctgca ttaactgtat tgcatgatgg cacagcaaca aatcctgttt
     7921 tagcaccacg tcttaaacat ttagatgatg ttcgcggtca ctctattatg atccacacgg
     7981 gtggtgataa tcactccgat catccagctc cacttggcgg tggcggccca cgtatggcat
     8041 gtggcgtgat taaataattc gattgttcga aacgaaaagt gcggtgaatt ttgaccgcac
     8101 ttttttgcta gatatttagc atttaattca cgacttcagc agtatagtga attaaattta
     8161 aaccagtaca gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctgaagc
     8221 accaagtgaa tcgattccgt actatttgag acctttgcaa aattcctttc cctcccgaca
//
LOCUS       DAXHTR010000095         8097 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.095, whole genome shotgun sequence.
ACCESSION   DAXHTR010000095 DAXHTR010000000
VERSION     DAXHTR010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.095"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(265..1650)
                     /gene="pntB"
                     /locus_tag="ACFNML_001459"
     CDS             complement(265..1650)
                     /gene="pntB"
                     /locus_tag="ACFNML_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFC6392822.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIEPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALAVSFVLLLVFVGINGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TLSSGSQEIGEYREVKAADIAEMLKGANNVIITPGYGMAVAQAQYPVAEITELLRKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(1716..2099)
                     /locus_tag="ACFNML_001460"
     CDS             complement(1716..2099)
                     /locus_tag="ACFNML_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFC6392823.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLARTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAGQATVNTLAGLFILFRLAFIWCYIA
                     DKAALRSLMWAGGFACTVGLFVAAA"
     gene            complement(2103..3644)
                     /locus_tag="ACFNML_001461"
     CDS             complement(2103..3644)
                     /locus_tag="ACFNML_001461"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFC6392824.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKDVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQISQGNGFVTLLAFIAILIASINIFGGFFVTRRMLN
                     MFRKG"
     gene            3934..4767
                     /gene="serB"
                     /locus_tag="ACFNML_001462"
     CDS             3934..4767
                     /gene="serB"
                     /locus_tag="ACFNML_001462"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HFC6392825.1"
                     /translation="MPHALVLQFPSAETLPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SEKDAALLGSRQIDHAVLSDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLREYR
                     SRLGLQPHQVLAMGDGANDIPMLKEAGIGVAYRAKPKVQAAADACINFGGLERVRGLF
                     G"
     gene            4831..6054
                     /locus_tag="ACFNML_001463"
     CDS             4831..6054
                     /locus_tag="ACFNML_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HFC6392826.1"
                     /translation="MGRRKLWFALAAAGVIGGLVGIVLTELMHFIQHTAYGYGTDGVS
                     ISFREGVAQASGMRRVAVLTLCGAVAGSGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGATAVFFQRTAQKFPFIKRDNIKIIPLAVCMFALIGVIAVWFP
                     EILGNGKAGNQLTFGGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6215..7795
                     /gene="purH"
                     /locus_tag="ACFNML_001464"
     CDS             6215..7795
                     /gene="purH"
                     /locus_tag="ACFNML_001464"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFC6392827.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDILSGTPEIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDIDPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYVTDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDICR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRITARKAQDANFDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTGIRHFRH"
BASE COUNT         1794 a         2189 c         2312 g         1802 t
ORIGIN      
        1 caagtggaaa aatacttggc agattatcag gcacacttgg aaaaagtgtt cggctaatta
       61 aaaatccatc ttcaacacgg agatggattt tgtttgtttc gttgattttg tgtcagtttc
      121 agatgtaggt gcttattcgg acgggccgtc tgaaaatgtt tgccccaatg caaaaaaatc
      181 actgcaaacc ttcataaacg gggtttgcag tgattttttc aaatcaaaca gattgaaaac
      241 ctgcgccgaa ttgttcagac ggcattattt tttcagttcg gacagaatgt catctacggt
      301 tttcttcgca tctccgaaac acatcacgct gttttcgttg aagaacagtg ggttttgtac
      361 acctgcgtag ccggtattca tcgagcgttt gaagacgacg acttcttttg ccttccacac
      421 ttccaacacg ggcatacccg caatcgggct gttcgggtcg gtttgggcgg cggggttgac
      481 ggtgtcgttc gcaccgatga ccaagaccac atcggtttcg gggaagtcgt cgttgatttc
      541 gtccatttcc aaaacgatgt cgtaggggac tttggcttcg gcgagcagta cgttcatatg
      601 accgggcagg cggccggcga cggggtggat gccgaagcgt acttcggtgc cgtttttacg
      661 taaaagctcg gtgatttcgg caacggggta ttgcgcttgt gcgactgcca taccgtagcc
      721 cggggtaatg atgacattgt ttgcgccttt cagcatttcg gcaatatcgg cagctttgac
      781 ttctcggtat tcccctatct cttggctgcc ggaagataat gtgccgctgt cgctgccgaa
      841 accgccggca attaccgaga caaacgagcg gttcatggct ttgcacataa tgtaggacag
      901 aatcgcgccg cttgagccga ccagcgcgcc ggtaacgatg agcaggtcgt tggagagcat
      961 gaagcctgcc gctgcggccg cccagccgga gtaggagttg agcatggaca cgaccacggg
     1021 catatctgcg ccgccgatgg aggcaaccaa gtgccagccg aatgcgaggg caatcagggt
     1081 cataatcagc aggatgaagc cgctaccgtt aatgccgacg aatacgagca gcaacacaaa
     1141 cgatacggcg agcgccagtg cgttgagctt gtgtttggcg ggcagttgca gcgggctgct
     1201 gctgattttg ccgttgagtt tgccgaatgc gaccagcgag ccggtaaagg ttaccgcgcc
     1261 gatgaaaatg cccaaataga cttcgaccag atggatggtg tgcatatcgt gcgaaacgtt
     1321 gcccggctcg atatagctgt tgaagccgac caaaaccgcc gctaggccga cgaagctgtg
     1381 cagcagggca atcagttcgg gcatttcggt catttccacc tttttggctt tgtagatgcc
     1441 gattgccgcg ccgatgagca tggcgatgat gatccagccc agtccgtggg tattgtcgga
     1501 aaaaacagtt acaaaaaggg cgaccgccat accggcgata ccggaatagc agccctgttt
     1561 ggcggtttcc tgtttggaca gccccgccag tgagaagatg aataaaattg cggcaacgat
     1621 atacgccgct gttacgagtc ctgaagacat ggaaattctc cgattttcga tgatttgttt
     1681 ttaatgccgt ctgaactgtt cagacggcat ctgtttcaag cagccgcgac aaacagcccg
     1741 acggtgcagg caaatccgcc cgcccacatc agcgagcgca atgctgcttt gtctgcgatg
     1801 tagcaccaga taaaggcgag gcggaacagg atgaacaggc cggcaagcgt gttgacggtt
     1861 gcttgtccgg cattgccggt tgcgtgtgcc gtcaaaacgg cggcggcaaa cggtgcaaag
     1921 gcttcaaaac cgttttgctg cgcggcgtgg gcacgggcgg ctgtgccttg cgtgcgcgcc
     1981 agaaaatcgc gcggattgtg gttgtcttta aaccggaatc cgcccgcttt tttggcatat
     2041 gccgcacaaa aaagcggcaa taagcaggca atcaggatgc accaataggc aaaagtcatg
     2101 ctttatcctt tcctaaacat attcaacatg cggcgcgtta cgaagaagcc gccgaagata
     2161 ttgatgctgg caatcaggat ggcgatgaag gcaagcaggg taacgaaacc gttgccttgg
     2221 ctgatttgca gcagcgcgcc gacgacgatg atgccggaga tggcgttggt taccgacatc
     2281 agcggtgtgt gcagcgagtg gctgacgttc caaacgacat agtagccgat gacgcaggcg
     2341 aggacgaaga cgataaagtg gttcaggaat gctgcgggtg cgaccgcgcc gacccacagt
     2401 accaacacgg cggcgatgac ggcgggcgcg agttttttcc acaggggaac gggtttcggt
     2461 tcgggcttgg cggcaggcgc ggctttttca gacggcgttt gctgcggttg ggcggaaact
     2521 tgaatcggcg gaggcgggaa ggtgatttcg ccgtcgcggg taacggtcat gttgcggata
     2581 atcacgtctt caaaatccag cgtgatttcg ccgtctttgt tcgggcttaa caacttggtc
     2641 aggttgacca ggttggtggc gtaaagctgg gaagactgtc cggcaaggcg gtttgccatg
     2701 tcggtgtagc cgatgatttt cacgccgttg ccggttacgg acaattcgcc cggtcgggtg
     2761 agttcgcagt tgccgcccgt cgccgccgcc aaatcgacga tgacggagcc ggatttcatg
     2821 ctttccacca tttctttggt aatcagcttg ggcgcgggtt tgcccggaat ggcggcggtg
     2881 gtgatgatga tgtccacgtc tttcgcctgc tcggcaaaga gcttcatctc ggcggcgata
     2941 aattcgtcgc tcatcacttt ggcatagccg tcgccgctgc cgcccgattc ttgcgggaag
     3001 tcgagtttca aaaacttgcc gcccatcgat tcgatttgtt ccgccacttc caagcgggta
     3061 tcgaacgcgc gcaccactgc gccgagcgag tttgccgtac cgatcgccgc caaacctgcc
     3121 acacctgcgc caatcaccaa aacctgcgcg ggcggcactt tgccggcggc ggtaatttga
     3181 ccggtgaaga aacggccgaa ggcgttggcg gcttcaatta cggcgcggta gccgctgatg
     3241 tttgccatcg aagacaaagc gtccaaagcc tgcgcgcgcg aaatgcgggg caccatatcc
     3301 atcgccagcg cgttcacttt cttggcgcgc aaggcttcga ccaaagcctc gttttggcgc
     3361 ggccacagga agctgacgat ggtttgacct tcgttcaaaa gcggcagttc ctgttcggac
     3421 ggcgcgttga ccttataaat caaagggcag acccaaaccg ccgctttgtc ggcaacggtt
     3481 gcgcctgctg tttggtaagc ggcatcgtcc aaacttgccg ccaaacctgc accgctttcg
     3541 acaacggttt caaagcccag tttgcccagc agggcgacgg tggcgggcgt gcaggcgacg
     3601 cgggtttcgc cggataatga ctcgcgtggg ataccgattt tcatctctga atcctttttc
     3661 gggtagttta tatgtatcgt gggttaaatt taaatcgggg cagggcggag caacgccgta
     3721 ccggtttaaa accgattcac ttcaaatgtt aatatatttt agataatccc cttataacga
     3781 attttcatca ggctggcaat agttgcggca ttttcccgtg ttgtccgaca catactgccg
     3841 tttgcgtttg aagtcatttt ccgattgcag tcccgcccgc cgtaacattc tttgccgttt
     3901 cactatataa tccgcatttt ttgagccgcc gttatgccgc acgccctcgt cctccaattt
     3961 ccctccgccg aaaccctgcc ttccgacttc ccctcacgcc tgcccgaacc tgattatgct
     4021 gatgaaaagc gtatgcgttt tatcgttgaa gaagggtttt ctttaagcga aaaagacgcg
     4081 gcgttacttg gcagccgtca aatcgaccac gccgtgttgt cggatatgga ttttggcgaa
     4141 ctcggtttga ttgtcagcga tatggattcg acgctgatta ccatcgaatg cgtcgatgaa
     4201 attgcggcag gcgtgggttt aaaagacaag gtagcggaaa ttaccgagcg ttcgatgcgc
     4261 ggagagttgg atttcgagca gtcgttgcgc agccgcgttg cgctgttggc gggattggac
     4321 gaacagattt tggcggacgt ttatgaaaac gttttgaagc tctcgcccgg tgcggaattc
     4381 ttgttggacg aatgcaaaag gcacgatgtg aaattcctgc tggtgtccgg cgggttcaca
     4441 ttttttaccg aaaggctgca acaacgactc ggcttcgaat accaacacgc caatgttttg
     4501 gaaattgaaa acggcaggtt gaccggtcgt ctgaaaggca gaatcatcga cgcgcaggca
     4561 aaggcggatt tattgcgcga ataccgcagc cgcctcggat tgcagccgca tcaggtgttg
     4621 gcgatgggcg acggtgcgaa cgatattccg atgctcaaag aagcgggcat aggcgtggct
     4681 taccgtgcca aaccgaaagt gcaggcagcc gccgatgcct gtatcaactt cggcggtttg
     4741 gagcgtgtac gcggcctgtt cggataggcg gataggaaac ggatgccgtc tgaaaggttt
     4801 tcagacggca tttgaacggc aggaacgaca gtgggacgca gaaaactttg gtttgccctg
     4861 gcagcagcgg gcgttatcgg cggtttggtc ggcattgtgc tgacggaact gatgcacttc
     4921 atacaacata cggcatacgg ttatggcacg gacggcgtgt ccatctcgtt ccgcgaaggt
     4981 gtggcacagg cttccggtat gcggcgcgtt gccgtgctga cgctgtgcgg cgcggtcgca
     5041 ggcagcggct ggtggttgct gaaacgtttc ggcaagccgc aaatcgaaat caaagccgcc
     5101 ttgaaacagc cgttgcaggg gctgccgttt ctgacgacgg ttttccatgt tctgctgcaa
     5161 atcataacgg tcggactcgg ttcaccgctc ggacgggaag tcgccccgcg tgaaatgact
     5221 gccgcgttcg cttttgccgg cggcaggcgg ttgggtttgg atgaagacga aatgcggctg
     5281 ctgattgctt gcgcttcggg tgcgggtttg gcggccgtgt ataacgtgcc gctcgcctcc
     5341 acacttttta tcctcgaagc catgctgggc gtgtggacgc agcaagccgt cgccgccgca
     5401 ttgctgactt ccgtcatcgc caccgccgtc gcgcgcatcg gcttaggcga cgtgcagcaa
     5461 tatcatcccg ccaaccttgc cgtaaacact tcattacttt ggttttccgc cgtcatcggc
     5521 ccgatactgg gcgcaaccgc cgtctttttc cagcgcaccg cccaaaaatt ccccttcatc
     5581 aagcgcgaca acatcaaaat cattcccttg gcagtctgca tgtttgcgct catcggcgtg
     5641 attgccgttt ggtttccaga aattttgggc aacggcaaag caggcaacca actgaccttt
     5701 ggcggtttga ccggttggca acacagcctt gagctgaccg ccgtcaaatg gctggttgtc
     5761 ttgatggcgt tggcggcagg cgcatacggc ggactgatta cgccgtctat gatgctcggc
     5821 agcaccattt cttttgcagc cgccgccgcg tggaacagcg ttttccccga aatgccgtct
     5881 ggaagcgcgg cagtcgtcgg agcggcggtg tttctcggcg tatcgctcaa aatgccgctg
     5941 accgccattg tgttcgtatt ggaactgacc tacgcgccgc tgtccctgct gctgcctttg
     6001 tgcatcggca tggcgggggc attgggaaca tcgggaaaaa tgggcttcaa ataaatgccg
     6061 acaaatgccg tctgaaaggt tttcagacgg catttctttg taaattgtca gcaatatctg
     6121 caaaaaccta ctgccaaaaa actagaaata gcggataata ccgccccgaa aaagttcccc
     6181 tttccccatc caactgtcag aaaaggaagc cccaatgtcc gtcatcaaac gcgccctgat
     6241 cagcctgtcc gacaaggcag gcgcggtcga atttgcccaa accctgacca agctcggtgt
     6301 cgaaatcctc tccaccggcg gcacggcgaa actgcttgcc gatgcgggcg ttcccgtgat
     6361 tgaagtcgcc gactataccg gcttccccga aatgctcgac ggccgcgtga aaacgctgca
     6421 tccgaaaatc cacggcggta ttttgggtcg gcgcgatttg ggcgaacacg tcgccaagat
     6481 ggaagaacac ggcatcggca atatcgacct tgtgtgcgtc aacctttatc ccttcgccgc
     6541 caccatcgcc aaaccaaact gcacactgga agacgcgatt gaaaacatcg acatcggcgg
     6601 cccgaccatg gtgcgttctg ccgcaaaaaa ctggaaacac gtcgccatcg ttaccgacac
     6661 cgccgacttc cccgccatcg ccgccgaact cgaagccaac aacggcgcgt tgagcgacaa
     6721 aacccgcttc aacctgtcgc gcaaagcgtt cagccacacc gcccaatacg acggcatgat
     6781 ttccaactac ctgacctcgc tttcagacga catcttgagc ggcacgcccg aaatcggtga
     6841 attcccgagc cagttcaacc aaagctggat taaagtgcaa gacatgcgct acggcgaaaa
     6901 cccgcatcag cgcgccgcgt tctaccgcga tattgacccc gccgcaggca gcctttccgc
     6961 ctacaaacaa ctgcaaggca aagaattgtc ttacaacaac atcgccgatg ccgatgccgc
     7021 atgggaagcc gtcaaatcct ttgacgcgcc agcctgcgtg atcgtcaaac acgccaatcc
     7081 gtgcggcgtc gccgttgcag ccgatacatt gaccgcctac aaactcgcct acgtcaccga
     7141 caccaccagc gcgttcggcg gcatcatcgc cttcaaccgc gaagtggacg gcgaaaccgt
     7201 caaacagatt accgacaacc agtttatgga agtcttgatg gcgccgaagt ttaccgccga
     7261 agccctcgaa atcgccgccg ccaagaaaaa cgtgcgcgta ttggaagtgc cgcttgaggc
     7321 aggcgcaaac cgcttcgaac tcaaacgcgt cggcggcgga ctgttggtac aaacgcccga
     7381 catctgccgc atcagccgcg ccgatttgaa agtcgtctcc aaacgccaac cgaccgagca
     7441 ggaatggaac gatttgctgt tcgtctggaa cgtcgcaaaa tacgtcaaat ccaacgccat
     7501 cgtgttcggc aaaggcggac aaacctacgg catcggtgca ggccaaatga gccgcgtgga
     7561 cagcacccgc atcaccgccc gcaaagcgca ggacgccaat ttcgacctca acggcgcgtg
     7621 tgccgcctcc gatgccttct tccccttccg cgacggcgtg gatgtgattg ccgaacaggg
     7681 cattaaagcc atcatccatc cggcaggttc gatgcgcgat caagaagtct tcgacgcggc
     7741 agacgaacac ggcattgcga tggtggtaac cggcatccgc cacttccgcc attaaggcag
     7801 atgaacaagg caatgccgtc tgaagggctt tcagacggca ttttgcgcta ttttgtgaag
     7861 gtagggatga cggttcgggt attcctgaca gggtggattt tcaaggtgtt gtatagggtg
     7921 taggaggatt cgtaaaaggt gggatgcagg gtgtgcttca gcccgctgca tcaaaaattt
     7981 ttggagaacc ggcgggagtc ggcggttttg gtttcggcgg ggacggtgga aatgggtaac
     8041 attgacggaa tcgacggaag cggtgggctg aagcccaccc ttgtatattg gaatcac
//
LOCUS       DAXHTR010000096         8096 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.096, whole genome shotgun sequence.
ACCESSION   DAXHTR010000096 DAXHTR010000000
VERSION     DAXHTR010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8096
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.096"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..573
                     /locus_tag="ACFNML_001465"
     CDS             <1..573
                     /locus_tag="ACFNML_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224706.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
                     /protein_id="HFC6392828.1"
                     /translation="DLGKLSPEAQLAAASLLQDSAFAVKDGINSARQWADAHPNITAT
                     AQTALAVAEAAGTVWRGKKVELNPTKWDWVKNNGYKTPAARPMQTLDGEMAGGNKPVV
                     KSIRPTTRDELRQALQEQGFRRTGSDEAQYETWKGPDGVKIDIRPNGEVIRTQRVPRT
                     DGVQGKYPQRQNYEGNPLPNNRHHSGYFIK"
     gene            570..887
                     /locus_tag="ACFNML_001466"
     CDS             570..887
                     /locus_tag="ACFNML_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392829.1"
                     /translation="MKKNIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNI
                     LNFKLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEP
                     LGK"
     gene            957..1250
                     /locus_tag="ACFNML_001467"
                     /pseudo
     CDS             957..1250
                     /locus_tag="ACFNML_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            1245..1697
                     /locus_tag="ACFNML_001468"
     CDS             1245..1697
                     /locus_tag="ACFNML_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392830.1"
                     /translation="MQTKALGTVDEIGDTVQQVGKQASGQKTSGGNPAIDSDPYSPSS
                     VAARIETGKVRSDLQIKDILSNTTQRSKTKGPAVQYDKVGDYNDALNDFNSLNVRNVQ
                     TRPNGTITGNLPDRRAVNARNDSSGGEPTLEITISNNRKIKIRYGNTR"
     gene            1701..2102
                     /locus_tag="ACFNML_001469"
     CDS             1701..2102
                     /locus_tag="ACFNML_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392831.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            2261..2603
                     /locus_tag="ACFNML_001470"
                     /pseudo
     CDS             2261..2603
                     /locus_tag="ACFNML_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2637..2738
                     /locus_tag="ACFNML_001471"
     CDS             2637..2738
                     /locus_tag="ACFNML_001471"
                     /inference="COORDINATES: protein motif:HMM:NF018014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392832.1"
                     /translation="MDDIRGIIQGAVNPLIYKVSKQKIIYNKLISGT"
     gene            2743..3099
                     /locus_tag="ACFNML_001472"
     CDS             2743..3099
                     /locus_tag="ACFNML_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMP12 family DNA mimic protein"
                     /protein_id="HFC6392833.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNKKTVIFHF"
     gene            3272..3646
                     /locus_tag="ACFNML_001473"
                     /pseudo
     CDS             3272..3646
                     /locus_tag="ACFNML_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3684..3946)
                     /locus_tag="ACFNML_001474"
                     /pseudo
     CDS             complement(3684..3946)
                     /locus_tag="ACFNML_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4026..4553)
                     /locus_tag="ACFNML_001475"
                     /pseudo
     CDS             complement(4026..4553)
                     /locus_tag="ACFNML_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684692.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            complement(4602..5588)
                     /locus_tag="ACFNML_001476"
     CDS             complement(4602..5588)
                     /locus_tag="ACFNML_001476"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFC6392834.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            complement(5628..6428)
                     /locus_tag="ACFNML_001477"
     CDS             complement(5628..6428)
                     /locus_tag="ACFNML_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HFC6392835.1"
                     /translation="MNAFNLIIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAAAALDLPVVRHPEAAKFIEG
                     ITQKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLNTYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            6553..7896
                     /gene="argG"
                     /locus_tag="ACFNML_001478"
     CDS             6553..7896
                     /gene="argG"
                     /locus_tag="ACFNML_001478"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFC6392836.1"
                     /translation="MNQNHTILQNLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIEPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLVTGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLSLGEGSVLPQLGNKK"
     gene            7971..>8096
                     /locus_tag="ACFNML_001479"
     CDS             7971..>8096
                     /locus_tag="ACFNML_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6392837.1"
                     /translation="MKEYKVVIYQESLLSSLFFGATKVNPVNFSAFLNKQTPEGWR"
BASE COUNT         2329 a         1759 c         1842 g         2166 t
ORIGIN      
        1 gatttaggaa aattaagtcc ggaagcacaa cttgctgccg cgagcctatt acaggacagt
       61 gcttttgcgg taaaagacgg catcaattcc gccagacaat gggctgatgc ccatccgaac
      121 attacagcta ctgcccaaac tgcccttgcc gtagcagagg ccgcaggtac ggtttggaga
      181 ggtaaaaaag tagaacttaa cccgactaaa tgggattggg ttaaaaataa cggctataaa
      241 acacctgctg cccgccctat gcagacgttg gacggtgaga tggcaggagg aaacaagcca
      301 gttgttaaat ctatcagacc aactacgcga gatgaattac gtcaagcatt gcaagaacaa
      361 ggttttagac gtactggttc agatgaggct caatatgaaa catggaaagg tcctgatggc
      421 gtgaaaatag atattcgtcc aaatggagag gttataagaa cccaaagagt gccgcgaacc
      481 gatggtgtac agggaaaata tccgcaacga caaaattatg aaggcaatcc attgccaaat
      541 aatcgtcatc attctggata ttttatcaaa tgaaaaaaaa tatttttcac aatgtaagcc
      601 tttatgaaat aatcttttcc gataatggaa atacccttac attatctttt acagatacaa
      661 ttgaaggtaa ttatttcgga tatatcaaat gcagtaatat tttgaatttt aaattagata
      721 caaataattt cgtagattat gaggataagg aagatagctt gtttcccttg tttatacccg
      781 aaatagagct atataaatac caattttata gtgaaattat tattgatgta gggattatta
      841 taaaaatatc tgctgaaaca attaattttg agccactggg aaaatagtaa ctgctttccc
      901 agcagccgta gcaactgtat ttttacccga cggggtaaaa atacagttgc tacatctctg
      961 cgcagcagac tctacaaggt attaataatt taggaaaatt aagcccggaa gcacaacttg
     1021 ctgccgcgag catattacag gacagtgctt ttgcggtaaa agacggcatc aattccgcca
     1081 gacaatgggc tgatgcccat ccgaatataa cagcaacagc ccaaactgcc cttgccgtag
     1141 cagaggccgc aggtacggtt tggagaggta aaaaagtaga acttaacccg accaaatggg
     1201 attgggttaa aaataccggc tataaaaaac ctgctgttcg ccatatgcag actaaggcgt
     1261 taggtacggt agatgaaatt ggcgatacag tacagcaggt tgggaaacag gctagcggac
     1321 aaaaaaccag cggtggtaat cctgcgattg atagcgaccc ctatagcccg agtagtgtgg
     1381 cagctcgcat agaaaccggt aaggtgcgca gtgatttaca aatcaaagac attttgagca
     1441 atactactca aaggagtaaa acaaaaggtc ccgctgttca gtatgataaa gtgggggatt
     1501 acaatgacgc actaaatgat tttaatagtc tgaatgttcg aaatgtacaa acacgtccta
     1561 atggaacgat aacgggcaat ttacctgata ggcgtgcggt taatgctcgt aatgatagta
     1621 gtggtggaga accaacactt gaaataacaa ttagtaataa ccgaaaaata aaaatcagat
     1681 atggaaatac acgataaatt atgaaattaa aaagcttaga tttcccaact ggctatttct
     1741 attttgataa tgcagcaata aactctgata aagtagaagt tatagcagtt ggttatagaa
     1801 atacggataa aaccataaaa atttttattg aagatgttat tcattttagg gttgttgatg
     1861 aatcgtattt tatagatact tttatggatt taatttcgga agatgcagat agagctttgc
     1921 ttcatgaaaa tggtggtcaa tctttttttg aacttcttga tgagtgttat gcggaatgga
     1981 tattgaaaga aagttatttt cctttgaata gagaattctt taaatactat atttttatgt
     2041 ttgagcaaac attcatagaa ataattggtt ctagtgcaac gtattcaatt attgagggct
     2101 agcgtaagat gagtaataag ttgcctatct ttctttcagg cagcctgaaa ataaaactac
     2161 ccaagttgat ggtgtacctg tatcagtgaa gggaaatttt gttgatggta aatttcgcat
     2221 tggtacggca acaatgaaat cattttaaat tgagctagaa atgaacctag aaaattatga
     2281 aaacatttta ataaaattac ttttttatca taacaactta gtaaatgaat attcttattt
     2341 tattgaaaat aaaaatttat ttaaaatagt atctcgaact aaaacgagta agggcttttt
     2401 ttcactatag aaaaaccatt aaattttcta agcaaaaaaa cttattttga gtttaatttt
     2461 aaatatttac actcagggaa agaacgcttt ggttcgttta tgtgctggat aaatactaat
     2521 ttaatggaat ttgagggggt ttttttaacg acctgctccc tgataatatg ataataaata
     2581 acttttttga aataaatgat taacgataca ccaataaaaa ttggtggggt aaccgtatgg
     2641 atgatattcg cggcatcatc caaggtgcgg ttaatccttt aatttacaag gtaagcaagc
     2701 aaaagattat ttacaacaaa ctcatatcag gaacttagac aaatgaatga acacaacctg
     2761 ttaattttct gtttaaaaga caatgtttca attagtgaat atactgaaat gattgattgg
     2821 gcttataaaa atattcaatc tgaaacagtt gtagaaatta cggaaagtca aattattgaa
     2881 tatcaaaatc gtggattatg gagacttgtt tctgaaatta ccgataattg gttatttgga
     2941 ccaagtgagg gggattggct aatagataag gaaagtattt tggctgtaaa agaaaaatta
     3001 caaaattcag atttttctac agagccctta gtgaaaaata ttattcatgt acttgaatat
     3061 gctataaaaa ataaaaaaac agtaattttt catttttgag actaatccaa tttttagtaa
     3121 tattgatgca gagcaagcag cattagatgc cgcaaacatg gggagaagct attcaattta
     3181 gaattaaaaa acaaattgaa aatgaactag caccaccaaa ttggtctacc cagtttccta
     3241 atggtagtat ttatgatcct aaggtaacga aatgattatt caaaatgaat ttaatttata
     3301 tcctagtaat atgcttcctg aaaggttttg ttatcctgaa aagtatgttc gtatctctaa
     3361 cgatacatct ttaatacctt atattcagcc acataatttt cactggtagt ttgagaatta
     3421 tggaacagaa ggggcagaag tagcttatat atttagaaat tctatcctgc ctgatttaaa
     3481 tcttatccca ttcgctagta atggagaatg ggaagcttat tttgatggta atgatgtaac
     3541 aggaaattct agggttattg tcattaattt agataatata gaaaaccatg aattttttaa
     3601 tagttttgaa gattggcttg aattagcaat taaggatact tggtaagcag ctatctataa
     3661 agagatgagg ctgccttgga caactaggat aaactcgatt ttactaattg ttttaaaatg
     3721 gaacaagaac ttttatttca ctgttgttaa aacgccattc gcactccttt aaatacagct
     3781 caaaatgcgc tttgggaatg ccgttaaact tgcgtaaatg acgttttgct tgattccaaa
     3841 agttctcagt tccattaata tggttttgtc gttcggcaaa atgtgtgctg tgattgatac
     3901 ggaaatggct aaattcgccc gcatccaata catcatagcc acgataacaa aatgagttta
     3961 ttttgtttat accgtcttag acgactttct ctcataggga taattctaac ttaatttgaa
     4021 tttccctagt gatctagggc agcccctaaa ttaataaagc agcacaactc cttttgccga
     4081 tgttccggac tgtcaaacga ctgttcctca tgccacatct ccatcaaggt acggataacc
     4141 cgctccgcct taccgttggt ctgcggacaa gcaaatcggg caagcctcca accaatccca
     4201 ttattataac aagctgcacc gaaagcatgt tggacggctc tttatattac ctatcattgt
     4261 cagagtaaac gtactcaatc aggtacaagc aggggtcagc cagatgttcg gtcagaaact
     4321 tggcagcact gtctgcggtt ttgtccggca aaatggcaga gtataaaaat cgtcaatagc
     4381 gacaaacagg taatctcgtt tatcggcggc cttctgtcct ttgagcaaca acaaccgatc
     4441 ggtatcagga tgaacaaaac ctcccgggac aacctgcctt ttacggcttt aagtgcacgg
     4501 taaatagtga cgcggctgac ttagtggcag catactgggg aggtgagtgt ttttgtgtat
     4561 atttttattt tggtattccc ttagaaatac tgtaaacaac gctaccggac ggcctgcagg
     4621 gcttcgcgca cgcttgcttt gagttctgcg ccgaagcgtc tgcccaagat tctgccgaaa
     4681 tcgtccttcg gagtgtaatc caccacatcg ggggctttga ccacgtctcg cgccacgctg
     4741 taaatattgc cgagtccgtc caccagcccg actttcagcg catccgcgcc tgtgtacacg
     4801 cgaccgctga acacgtcggg atattgtcgg aatttgaggc ggccgccgcg tccggttttg
     4861 acggctttga tgaactcacc gtgtatgccg gtcagcattt cttcccagat ttttgactgt
     4921 tcgggcgttt cgggcgaaaa cggatcgccc atgcctttgt tgctgcctgc aattttaacc
     4981 ctgcgtttca cgccgatttt ttccatcagg ccggtcgcgt cgaaactgct gccgataacg
     5041 ccgatgctgc cgacgatgct ggacgggtcg gcatagattt tgtccgccgc cgccgcgatg
     5101 tagtagcagc cggacgcgca catatcttcc gccacgagat aaacgggaat gccggggtgc
     5161 tgcgccttca gacggcgtat ttcttcaaaa gcggtgttgg agacgacggg cgaaccgccg
     5221 gggctgttgg cgcggatgac gatggctttt gcctgcgggt ttttgtaggc ggcctccata
     5281 ccgtctttga gttttttgac ctggtcttct acaccgttgc cgatttcgcc gtacaggttg
     5341 acgactgcgg tatgcggcgt gttgcccgcc aactgcaatg cggcttcgtc ttttcggaaa
     5401 atgcctgcaa tcagggcaac cagaatcagg gtgctgacgg cgcgccagat gtttttccac
     5461 atccgctccc tgcgcctgtc ctgataggcg gacagcagca cttcgcgcat gatgtcgcgc
     5521 tcccataagg tttcccccgc attttttgct tcgggtgctt cgttttctct tctgattcgg
     5581 tattgcatgg ttttccttaa atattgtccg atttgggcaa acggttttca gtttacccga
     5641 tttttcagct ctgctcccaa tccgtccaag ctgtgcaaca cttccgccca cgccgcgtcc
     5701 aaaaggttga cggcttctcc ttcggctttg atgccgaact caatgtgcgg tttgacctgc
     5761 gtgccgtctg aatgcgtcca accgacgctg ggcaggctgt acgaacgcac accgggataa
     5821 gtttgctcga tatgctccat aagcggcgta atgcgcgatt cgggctgctc aaacacatac
     5881 acgctgcggc tgccgcgttc ggtttggttg aagcggtcgg cgtaataagt attcaaaacc
     5941 cattccgcca tcgggtgcgc catcacagga aagccgggga agaaataatg ctcgcggatg
     6001 gaaaatccgg cgatgttgtt gaacgggttg ggcacaagct ctgcgccctc ggggaaatcc
     6061 gccattttca ggcgttgggc gtgttccggc gaatcaagcg gctcgccgcg tttctgggtt
     6121 atgccttcga taaacttggc ggcttcagga tggcggacga caggcaaatc caaagcagca
     6181 gccgcggctt ggcgcgtgtg gtcgtcgggc gtggcgccga tgccgccggt aacaaacgtc
     6241 ggtatgccgt ctgaaaagct gcggcgcagt tgcctgacca gtaaatcggg ttcgtcgggt
     6301 agatattgca cctgattgag cttcagccct ttggattcga gcagggattt gaaaaaggcg
     6361 aaatgcttgt cttggcggct gccgtgcagg atttcgtcgc cgatgatgat gaggttgaac
     6421 gcgttcataa tgttttcggt tttaaatacc gcctgaaacg gcggaatacc cgttcagacg
     6481 gcatgatggc tgacaatatt tcctatttat aagataatgg ctttgatttt tcaagctacc
     6541 gaggattttt tcatgaacca aaaccatact attttacaaa atctccccgt cggtcagaaa
     6601 gtcggcatcg ccttctccgg cggacttgat acctccgccg cgctgttgtg gatgaaactc
     6661 aaaggcgcgc tgccttatgc ctacactgcc aacctcggcc agcccgacga agacgattac
     6721 aacgccattc ccaaaaaagc gatggaatac ggcgcggaaa acgcccgctt aatcgactgc
     6781 cgcgcgcagt tggcacatga aggcatcgcc gccatccaat gcggcgcgtt ccacgtttcc
     6841 accggcggca tcgcctattt caacaccacg cctctgggcc gcgccgtgac cggcaccatg
     6901 ctcgtttccg caatgaaaga agacgatgtg aatatttggg gcgacggcag cacctacaaa
     6961 ggcaacgaca tcgagcgttt ctaccgctac ggtttgctca ccaatcccgc gctgaaaatc
     7021 tacaaaccct ggctcgatca gcaatttatc gacgaactcg gcggccgtca cgaaatgagc
     7081 gaatttctga ttgccaacgg cttcaactac aaaatgtcgg tggaaaaagc ctactccacc
     7141 gattccaata tgttgggtgc cacccacgaa gccaaagact tggaattttt gaactcgggc
     7201 atcaaaatcg tcaaacccat tatgggcgtt gccttttggg acgaaaacgt cgaaatcgaa
     7261 cccgaagaag tcagcgtacg ctttgaagaa ggcgtgccgg ttgcactgaa cggcaaagaa
     7321 tacgctgatc ccgtcgaact cttcctcgaa gccaaccgca tcggcggccg ccacggtctg
     7381 ggtatgagcg accaaatcga aaaccgcatc atcgaagcca aatcgcgcgg catctacgaa
     7441 gccccgggaa tggcgttgtt ccacatcgcc tacgaacgct tggtaaccgg catccacaac
     7501 gaggacacca tcgaacaata ccgcatcaac ggcctgcgcc tcggacgttt gctctaccaa
     7561 ggccgctggt tcgacagcca agccctgatg ttgcgcgaaa ccgcccaacg ctgggtcgct
     7621 aaagccgtta ccggcgaagt taccctcgaa ctgcggcgcg gcaacgacta ctcgattctg
     7681 aacaccgaat cgcccaatct gacctaccaa cccgaacgcc tgagtatgga aaaagtcgaa
     7741 ggtgcggcgt ttaccccgct agaccgcatc ggacagctca cgatgcgcaa ccttgacatc
     7801 accgacaccc gcgccaaact gggtatctac tcgcaaagcg gtttgctctc gctgggcgaa
     7861 ggctcggtat tgccgcagtt gggcaataag aaataaggtt tgctgttttg catcattagc
     7921 aacttaaggg gtcgtctgaa aagatgatcc cttatgttaa aaggaatcct atgaaagaat
     7981 acaaagtcgt catttatcag gaaagcctgt tgtccagcct gtttttcggc gcgacaaagg
     8041 tcaaccccgt caatttcagc gcgttcctca acaaacaaac ccccgaaggc tggcgg
//
LOCUS       DAXHTR010000097         8038 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.097, whole genome shotgun sequence.
ACCESSION   DAXHTR010000097 DAXHTR010000000
VERSION     DAXHTR010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.097"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            205..1017
                     /locus_tag="ACFNML_001480"
     CDS             205..1017
                     /locus_tag="ACFNML_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HFC6392838.1"
                     /translation="MASKPFLKWAGGKHKLVPFIEHNLPTPTRKRLIEPFCGSAALSL
                     ALDFEQYLLNDINADLIGLFRILKEEKSGFIDYARSFFISENNSDSRFYELREQFNFS
                     KNLHERSALFIYLNRHAFNGLCRYNSKGVFNVPFGRYKSPYFPQQEMEGFIQKSDRVE
                     LMCGDFQMILSQTDNTDTVYCDPPYAPLSPTASFTTYAKEGFNLDNQIRLAQSAQQIA
                     PDSQGVLISNHDTEFTRDIYSFAILKTIEVQRNIAAKGSSRKKVGELLAIYD"
     gene            1010..1819
                     /locus_tag="ACFNML_001481"
     CDS             1010..1819
                     /locus_tag="ACFNML_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFC6392839.1"
                     /translation="MIDFDKPAEEDAIYQSRLKKIVSDDLVLYNENSLNVMRKILEKY
                     PNGCFDMIFADPPYFLSNDGFSCQNGQMVSVNKGNWDKSKGMAADLEFYEEWLRLCYA
                     LLKPNGTIWVCGTFHNIYLIGYLMQTIGYHILNNITWEKPNPPPNLSCRFFTHSTETI
                     LWAKKNKKAKHTFHYEMMKAQNDGKQMKCVWTFPPPNKAEKTFGKHPTQKPLPLLERC
                     ILSASNIGDLIFDPFMGSGTTGVAALKHGRKFCGCELEEDFFELAKKRLEK"
     gene            1816..2370
                     /locus_tag="ACFNML_001482"
     CDS             1816..2370
                     /locus_tag="ACFNML_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392840.1"
                     /translation="MIIGGIGGARTQTGLRFEERTDLRKLFEEIPGYDLRKTYDNAGY
                     EVWFNGELKAYCFKKYEFYRFLKRPGYSINWKDHLSKRLLPDNGLFIIIRDTLFIIEI
                     KFQQTPGSVDEKLQTCDFKRKQYTKLVHSLGWRVEYVYVLNDWFTKPEYKDVLDYIIS
                     VNCHYQFNTIPLRWFGLPDGETNE"
     gene            complement(3245..3556)
                     /locus_tag="ACFNML_001483"
     CDS             complement(3245..3556)
                     /locus_tag="ACFNML_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6392841.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(3553..3920)
                     /locus_tag="ACFNML_001484"
                     /pseudo
     CDS             complement(3553..3920)
                     /locus_tag="ACFNML_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(4736..4821)
                     /locus_tag="ACFNML_001485"
     tRNA            complement(4736..4821)
                     /locus_tag="ACFNML_001485"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4785..4787),aa:Leu,seq:gag)
     gene            complement(4835..5185)
                     /gene="secG"
                     /locus_tag="ACFNML_001486"
     CDS             complement(4835..5185)
                     /gene="secG"
                     /locus_tag="ACFNML_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFC6392842.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(5192..5965)
                     /gene="tpiA"
                     /locus_tag="ACFNML_001487"
     CDS             complement(5192..5965)
                     /gene="tpiA"
                     /locus_tag="ACFNML_001487"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFC6392843.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            6125..6652
                     /locus_tag="ACFNML_001488"
     CDS             6125..6652
                     /locus_tag="ACFNML_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HFC6392844.1"
                     /translation="MYATIGDVMHALHFSASDKAALYREVLPQIESVVADETDWVANL
                     ANTAAVLKEAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGT
                     VVVGDVDAHPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGEL
                     AKILEKRFEASRQAA"
     gene            6754..7410
                     /locus_tag="ACFNML_001489"
     CDS             6754..7410
                     /locus_tag="ACFNML_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFC6392845.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALEEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLSLENIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRRGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            7489..7812
                     /locus_tag="ACFNML_001490"
     CDS             7489..7812
                     /locus_tag="ACFNML_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFC6392846.1"
                     /translation="MDIVQLGSAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAAEIDG
                     NMMRY"
BASE COUNT         1930 a         1775 c         2072 g         2261 t
ORIGIN      
        1 ggccgtctga aacttggaaa cagggtttca gacggccttt ttaaggcaga tggagttgat
       61 aagtccaata acatattgga atggaaaggt tctattggat ttagactcac tctacacacg
      121 cccccaaata taaatccatt gtattcctca cgatgttttg gcacaattcc aagccaatca
      181 accacgcttg tactcggcat cctaatggct tcaaaacctt ttctaaaatg ggcaggcggc
      241 aaacataaac ttgtcccctt tatcgaacac aatctaccta ctcccacacg aaaacgcctg
      301 attgaaccct tttgcggttc tgccgctttg tctcttgcat tggatttcga acaatatctg
      361 ctcaatgata ttaacgctga cttaatcggc ttattccgta tcctaaaaga agaaaaatcc
      421 ggattcatcg actacgcccg ctctttcttt atttccgaaa acaatagcga ttcaagattc
      481 tatgaattgc gggaacaatt caacttcagc aaaaatctgc acgaacgctc ggcactattt
      541 atttatttga accgtcatgc ttttaacggt ttatgccgtt acaacagcaa aggggtattc
      601 aatgtgccgt tcggacgcta caaatcgccg tactttcccc aacaagaaat ggaaggtttc
      661 attcagaaat ctgacagagt tgaattaatg tgcggagatt ttcaaatgat tctatctcag
      721 acagacaata ccgatacggt ttattgcgat cccccttacg cgcctttaag cccaaccgct
      781 tcatttacca cttatgccaa agaaggtttc aatttagaca atcaaatccg tcttgcccag
      841 tcagcccaac aaattgcgcc cgattcccaa ggcgttttga tttcaaatca tgatactgaa
      901 ttcacaaggg atatttattc ttttgctatt ctgaaaacaa ttgaagtaca acgcaatatt
      961 gccgcaaaag gcagcagccg taaaaaagtc ggagaattat tggcaattta tgattgattt
     1021 tgacaaaccg gctgaagaag acgccattta tcaaagtcgt ttgaaaaaaa tcgtttcaga
     1081 cgaccttgtc ctctacaacg aaaattccct taacgtcatg cggaagatat tggaaaaata
     1141 tccaaacggc tgttttgata tgattttcgc agacccgcct tactttcttt ccaatgacgg
     1201 cttcagttgt caaaacggac aaatggtttc cgtcaataag ggcaactggg ataaatccaa
     1261 aggaatggcg gcagatttgg aattctacga agaatggctc cgactgtgtt acgccttatt
     1321 aaaaccaaac ggcacaattt gggtttgcgg cacattccat aatatttatt taatcggcta
     1381 cctgatgcaa actatcggat accatatttt gaacaatatt acttgggaaa aacccaatcc
     1441 tccccctaat ttgtcctgcc gtttttttac ccattcgaca gaaacaatct tatgggcaaa
     1501 gaaaaataaa aaagccaaac acacgtttca ttatgaaatg atgaaagcac aaaatgatgg
     1561 caaacagatg aaatgtgttt ggacatttcc tcctccaaac aaagccgaaa aaacattcgg
     1621 caaacatccg acacaaaaac cactcccctt gcttgaacgc tgcatactct cagcttcaaa
     1681 catcggagat ttaatttttg accctttcat gggcagcggc acaacaggcg ttgccgcctt
     1741 aaaacatggt cggaaatttt gtggttgcga actggaagaa gatttttttg aattagcaaa
     1801 aaaaaggtta gaaaaatgat tattggcgga atcggtggtg caaggacaca aacaggactc
     1861 agattcgaag aacgtacaga cttacgaaag ttgtttgaag aaattcccgg gtacgactta
     1921 agaaaaacat atgataatgc gggttatgaa gtttggttca atggagaatt gaaggcttat
     1981 tgttttaaaa aatatgagtt ttaccgattt ttgaaaagac cgggatacag tattaattgg
     2041 aaagaccatc tgtctaaaag attactaccc gacaatggct tatttatcat catccgtgat
     2101 accttattta ttattgagat caaattccaa caaactcccg gttcagtaga tgagaaatta
     2161 caaacttgcg attttaagag aaaacaatat acaaaattgg ttcactcttt aggttggcgg
     2221 gttgaatatg tctatgtttt gaatgattgg tttacaaagc cggaatataa agatgtttta
     2281 gattatatta tcagtgttaa ctgccattac caatttaaca cgattccctt aaggtggttt
     2341 ggactgcctg atggtgaaac aaatgaataa ataccatatt taaaacacag ccgttcaagg
     2401 tcgtctgaat atcatttcag acgacctttc accaatcccc gccgttttcc atctttctgc
     2461 tattgtgata aagtagcccg accttttgtt caaaatacgc ggattccgcg acactgaatg
     2521 cagcatctgt caaagattat ttgaaaacgg tcgagttaga caagtctgcc gacaatgtcg
     2581 ataccacatc caaaactatc cgcagggttt aggcggtatt ccagagtacg gtgtcttatg
     2641 ctgatgctgt aaacaatgcc cgtatagaaa atctaaaacg gttgttgccg accgttatgc
     2701 cggtttatga gcaaagtgtc agaaataagg ggcgcgttaa tatagtggat taaatttaaa
     2761 tcaggacaag gcgacgaagc cgcagacagt acagatagta cggcaaggcg aggcaacgct
     2821 gtactggttt ttgttaatcc actataaaaa acgtcggcgt taagggaagg ggatcgagaa
     2881 tttgagccgt tgtttcaaaa tgccgtctga aatcgtttga gattcagacg gcatttaaac
     2941 agttctgcgc ccgcccgttt tgccttcaag cccgcgccgc ttaaatccaa tcccccctgc
     3001 aacgggcgca aaaaaatctc cgtgcctcgc ggtcggggat gtttcgggtt tcacgcccgt
     3061 ctgcgccgtg ccttgctgtc gggctatgcc gtctgcaccg ccgccgtttc ggcttggttt
     3121 gatgcggtcg ggcatttatg cggctacgcc ctgatattca gttcggcggt cttgcctgtt
     3181 ttggcaggca tattcagcag cgcgtctatt gtgaacttgc tgattttgcc gtttgccaag
     3241 tcggctacgc aatggcttcg atacccttgt ttttgacgat ggtcagcagc ttcaacgccg
     3301 cgccgctggg ctttttaacg ccccgctccc aagccgaaac gtgatttttt cccacgttga
     3361 gatagatggc gaaagcggct tgcgatagtg cctccttctc cctgattgcc ttgatgtctc
     3421 cgccgctcaa cggtttgatt tcggtcaggc aggacttgtc aaagccgcgc atagtctttt
     3481 tgtcgattgc gccgatgttg taagcccctt ccatcatttc atgaatggcg gctaatgcct
     3541 cgcttttgta tttcatgatt ctatctccgt gataatgccg tcttctttca aagccgccag
     3601 ctctgcccgc gtgtatttca gataatatgc ggcggctttt cggtaaacgt ccagttcttt
     3661 atccgaaatg ttttccctgt cgttcttggc aaaggcgtaa acgaaaatgc cctatctgcc
     3721 tgtttgaaca atatcaggct gcgatagccg ccgcttctgc cttgtccttg ccttgctatg
     3781 cgctgcttaa tcacaccgcc gcctaaatct gcgtctatca gcccgttatc cgcccgctct
     3841 accgcttcca gcagctcgga atcgttgatt ttgtgcttct tggcaaattt cactatccat
     3901 tggtttttga atatccgcat tgtgtagccg ttgattagtg atacttggaa ttataccaga
     3961 gacagacaac acggcaagct gcctttttta tgccccgcaa tttctccgtt tatgccgcct
     4021 gaaacactgg cggctaacct gattattttt taatcaatcg cttttaatgg ctcaaattgc
     4081 gtttttagcg cgtttcgctg tcttcctatg tccccatata taaccaagtg gtttaaccgc
     4141 cattcctgct caaaataggc gcggttccgt gcgttttggc ttttcattcc gctacgctcc
     4201 cactgccaag acggtgaaaa accggaaaac cccaaaagtt gttaccgatt cgttaccatt
     4261 tgttaccgca tttgttaccg catttgttac caatttttta tctttttctt attttatagt
     4321 gaattaaatt taaaccggta cggcaaggcg aggcaacgcc gtaccggttt aaatttaatc
     4381 cactatagta taggtttttt ccaaaaaccc aaaaaaaaac cgctctgcat cttggacggt
     4441 ttccccctca ttccctacgg tgcgtcagta ttccccgcaa gctatttccc gcttggcatt
     4501 cctgatttgg tggtcgttca gctttatttt tacggtattt tctcgcggta ttttcggatg
     4561 ctgtaaaaaa tcaggttagt tcaattaggg tggattggac gggattgtac ggtcaggaag
     4621 ggggacggca ggataacaat cagcctgaaa gccttgtatt gattgggatt ggttgacggc
     4681 attagacagg attaaacgaa aaaaaacgct tagaatttct aagcgttttg tgtgttggtg
     4741 ccgacagcga gatttgaact cgcacagcct acggccacta ccccctcaag atagcgtgtc
     4801 taccaatttc accatgtcgg catttgaaaa actgttattt ctgctgctga ggaacagggg
     4861 cagaaggttc ggtattgctt acgggtttgg gtgcttgctg agtttgttgt acgttgctga
     4921 agtccaaacc gtgttttgtt gtgtgggtgt gaatatacac catagccatg caggttgcaa
     4981 agaaaaatgt tgctgcaacg gcggtcgagc ggctgaggaa gttagcgttg ccggcagagc
     5041 cgaatacgcc ttgcgcgctg ccgcttcccg atccgaaagt cgcgccggca tccgcgcctt
     5101 tgccgtgttg gagcaatact aacacgatga cggccaaagc ggaaattata ttaacaatcc
     5161 aaattagggt tttgaaggct tccatatttt tctacgcatt ttgtgcggca ctgatgatgg
     5221 cggtaaagga gtcgtacgac aatgacgcgc cgccgacgag tgcgccgtcc acataaggta
     5281 ctgcgaagat gtcggccgcg ttgtccgctt tcacacttcc gccgtaaagg acgcggattt
     5341 taacatcgct tccgcacaaa gacaagattt ctttgtagat gaatgcgtgc atatcggcaa
     5401 tctgttcgac ggtggcgact ttgccggtgc cgatcgccca gacgggttcg taggcgacgg
     5461 cgatgttttt ggtatccagc ccttgcagga tggaaagctg atgggcgatg acttcgtgtt
     5521 ctttgccggc ttcgcgctct tcgaggcttt cgccgacgca caataacggg atgagtccga
     5581 cgttgaggac gttttccatt ttgcggcgtt ggatttcgtt tttttcgccg aaataaaggc
     5641 tgcgttcgga gtgtccgatg aggacgatgt ctgtgccggt gtcggcgagc atttcggcgg
     5701 acacttcgcc ggtgtacgcg ccgttgtcgg ggaagcggct cacgtcttgg gcgcaggtga
     5761 ggatgcggtt gtttaagacg atttgcatgg cgttgtgcag ttgcagcagg taaacggtcg
     5821 gggcggcgag tccgatgagg acgcgttcgg cggtggggag gatgcggaag cggtgcatga
     5881 gcgaattgtt gttctggagc cggccgttca ttttccagtt gccgatgacc catttttgat
     5941 cccacattcc gatttggtga tacatctttt ttgctccgtg tcgtgttttt tctgtctgcc
     6001 gcgtgtggcg cgttgcaatg tgaagtttag tggatatgcg gcgggttcgc aacttggggc
     6061 gggcggctgc gggggcggtt tggaatgttg tttcgggcag gttgttttat aatggccgcc
     6121 tgatatgtat gcaactatag gagatgtgat gcacgcgctt catttttcgg cttcggacaa
     6181 ggccgcgctt tatcgggagg tgttgccgca gattgagtct gtggtggcgg acgagacgga
     6241 ttgggtggcg aatttggcga acacggcggc ggttttgaag gaggcgttcg gttggttttg
     6301 ggtgggtttt tatttggtcg atacgcgttc ggacgaattg gttttggcac cgtttcaggg
     6361 gcctttggcg tgtacgcgga ttccgttcgg tcgcggggtg tgcggtcagg cttgggcgaa
     6421 gggcgggacg gtggtcgtcg gggatgtgga cgcgcatccc gaccatattg cctgttcgtc
     6481 tttgtcgcgt tcggagattg tcgtgccgct gttttcagac ggccgctgta tcggcgtgtt
     6541 ggacgcggac agcgaacatt tggcgcagtt tgatgagaca gatgctttgt atttgggcga
     6601 actggcgaag attttggaga agcggtttga ggcttcgcgc caggcggctt gagtgatgga
     6661 aaggggcggg cggcgcgtgc cgaagttggc gcggcgggag tgtggtttta taatgcctgc
     6721 cattgttgaa acaattattt gacggagcac taaatggatt ttgaaaaagc gcggttcaat
     6781 atggtcgaac agcaaatccg tccttgggat gtattggatt ttgacgtttt ggacgctttg
     6841 gaggagattc cgcgcgagct ttttgtcgat gaggatttgc agggtttggc gtatgcggat
     6901 atggcgctgc cgcttgccaa cggtcataag atgctggagc cgaaagtcgt ggcgcggctg
     6961 gcgcagggct tgaagctgac gaaaaacgat acggttttgg aaatcggcac gggttcgggc
     7021 tatgcgaccg cgcttttggc aaaattggct ggtcgtgtgg tgtcggacga catcgatgcc
     7081 gaacagcaaa accgcgccaa agcggtgttg gacggcttgt ctttggaaaa cattgattat
     7141 gtgcaaaaca acgggttgac cgaactttcc gcaggtgcgc cgtttgatgc ggtttatgtc
     7201 ggcggtgcgg taaaccttgt gcctgaagtg ttgaaagaac agttgaaaga tggcggacgc
     7261 atggtggtta tcgtaggacg caagcctgtt caacgcgcgc ttttgattac gcgcaggggc
     7321 gatgtgtttg aagagaaggt gctgttcgat accttggtgg cgcatttgga tgataaggat
     7381 gccgatccgt ttggtggttt tcagttttga tgttcggatt gcgatgccgt ctgaaagcgg
     7441 gtttggggct tcagacggca ttttgcttgg ttttttttgg ggggtgcgat ggatattgtg
     7501 caacttgggt cggcggaatt gaaggcgtgg atggacgaag ggcggatgtt ttgtttgttg
     7561 gacgtgcgta cggatgaaga aacggcggtt tgttcgctgc caaatgcgct gcatatcccg
     7621 atgaatctga ttccgctgcg gcagaacgag ctgccggatg atgtgccgct tgtggtgtat
     7681 tgccaccacg gtatccgcag cctgcatacg gcgatgtatc tggcggacgc gggttttgaa
     7741 aacctgtaca accttcaggg cggcatcgat gcatgggcgg ccgagattga cggcaacatg
     7801 atgcgttatt gaggcaggca tttcagacgg cattccttaa atgcgtgtac ctgtggtgtt
     7861 ccaaaaggtc gtctgaaagt gcagcttctg cgaagctaaa gcgcggtatc aacgaagtta
     7921 aaatttgcct gaaccttaaa ggcagcctgc accccaatcc cctctcccgt gggagagggc
     7981 taggacgagg ctggcaaact gaaagtttgc ttgggcggca ttttcaatgt gcaggctg
//
LOCUS       DAXHTR010000098         8034 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.098, whole genome shotgun sequence.
ACCESSION   DAXHTR010000098 DAXHTR010000000
VERSION     DAXHTR010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8034
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.098"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(113..691)
                     /gene="grpE"
                     /locus_tag="ACFNML_001491"
     CDS             complement(113..691)
                     /gene="grpE"
                     /locus_tag="ACFNML_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFC6392847.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            875..1693
                     /gene="cysE"
                     /locus_tag="ACFNML_001492"
     CDS             875..1693
                     /gene="cysE"
                     /locus_tag="ACFNML_001492"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFC6392848.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAVSAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            complement(2050..3561)
                     /gene="ubiB"
                     /locus_tag="ACFNML_001493"
     CDS             complement(2050..3561)
                     /gene="ubiB"
                     /locus_tag="ACFNML_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFC6392849.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQSIEKLYAEFETEPIASASIAQVHKARLHSGERVAVKVLRPNLLPLIEQ
                     DLSLMRFGAAWVERLFSDGRRLKPLEVVAEFDKYLHDELDLMREAANAGQLGRNFQNS
                     NMLIVPKVFYDYCTSDVLTIEWMDGTPVADIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWMNGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
     gene            complement(3596..4060)
                     /locus_tag="ACFNML_001494"
     CDS             complement(3596..4060)
                     /locus_tag="ACFNML_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HFC6392850.1"
                     /translation="MIEMSKDYRNDLYDVYVSYPPQVDRGLIRECLKENLGEEKAEGL
                     IESLDSKPQVLVEEKCTWAKREELHDYFSYLGLDIITRRYMELETVVPPEEGEGEGEG
                     ADGEMPEYLELHGGREDDISAPSQPEPPSRNIKLLVFGLLIAFLGYLLGKIF"
     gene            complement(4057..4203)
                     /locus_tag="ACFNML_001495"
     CDS             complement(4057..4203)
                     /locus_tag="ACFNML_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392851.1"
                     /translation="MPSERSDGIFTFRKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            complement(4220..4558)
                     /gene="erpA"
                     /locus_tag="ACFNML_001496"
     CDS             complement(4220..4558)
                     /gene="erpA"
                     /locus_tag="ACFNML_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFC6392852.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            complement(4690..5376)
                     /locus_tag="ACFNML_001497"
     CDS             complement(4690..5376)
                     /locus_tag="ACFNML_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFC6392853.1"
                     /translation="METFKDRLVFLWKSEARQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            5564..5836
                     /locus_tag="ACFNML_001498"
     CDS             5564..5836
                     /locus_tag="ACFNML_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392854.1"
                     /translation="MKKTGKYLIYTAAFTAFCFAFQENRSEAKQPDITLSASLCEQFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            6044..7972
                     /gene="dnaK"
                     /locus_tag="ACFNML_001499"
     CDS             6044..7972
                     /gene="dnaK"
                     /locus_tag="ACFNML_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFC6392855.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKDFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKTEALGAASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
BASE COUNT         2204 a         2220 c         1789 g         1821 t
ORIGIN      
        1 aatgccgtct gaagcccgac aggcgggttc agacggcatt gccgctttac ttcgatcggt
       61 ttatacgcaa aacgcgcttc aggaaataaa tcagattatt ccccagacgc cttcaggctt
      121 ccttctgcgc caccgtaacc atagccgggc gcaacacgcg gtcggacagc gtataaccct
      181 tcttcatcac acccaccacg gtattcggct cctgttcgct tgccaccgcc tgcatcgcct
      241 gatggatatt cggatcgagc ttatcgcccg ctttagggtt gatttccttg atttgcgtag
      301 catcaaatgc tttctgcaac tcgttcaaag tcatctgcac gcccattttc agcgcatcga
      361 aattgccgct ctgatccaaa agcgccattt ccagataatc cttgaccggc agcatttcca
      421 cggcaaactt ctgtccggcg aacttgtgcg tatccgcaat ttcctgctgg tggcggcggc
      481 gcaggttttg ctcgtttgcc aaagcgcgca gctgctcgtc tttcaactgc gcttccagct
      541 cggcaatccg cgcctgcaaa tcctcataag ccggctctgc ggcagcctgt tcctgcacac
      601 cgtccgcatt tcctactgtc tcgacggttt ccaccgcctc cacattttca accgcttctt
      661 cactgttttg ctgctgtgtc tgttcgctca tatcgtatcc cttaaaacaa attggaaatc
      721 aaaatccagt tattacccgc gcaagataag gacattttca agaaacttca agcaaaagca
      781 gagaatttca aattcatttt caaatcccct accgaaaaaa taatatagac ggtaagatac
      841 tgccgaccat atcgattttc aaacgtcatc actcatgaaa aaagaccatt taaacacaac
      901 cggtttcgac ctctggcaca ccatccgcga agaaaccgcg gccgcggtct ccgccgaacc
      961 gatgctggca agttttttgc accaaaccgt gttgcgccac gagtccctcg gctccgtcct
     1021 tgcctaccac ctttccagca aactcggcag cccgattatg gacgtgcgcg cgctgtttga
     1081 aatctaccag caggcgttgg gcagcgacac ccaaatcggc aaatgcgtcg aagcagactt
     1141 aaaagccatc tacgaacgcg atcccgcctg cgacgaatat tcgctgccgc ttttatattt
     1201 caaaggcttc cacgcgattc aggcacaccg catcaaccat cggctgtatc tcgacgggcg
     1261 caaaacgctg gcgtatttcc tgcaaaaccg tatgtccgaa gtattcggcg tggacatcca
     1321 tcccgccgcc cgtttcggat acgggctgat gctcgaccac gccaccggct ttgttgccgg
     1381 cgaaaccgcc gtgttgggca acaatatttc gattttgcac ggcgtaacgc tcggcggttc
     1441 gggcaaagaa ggcggcgacc gccaccctaa aatcggcgac ggcgtgatga tcggcgcaaa
     1501 cgcctcgata ttgggcaata tccgcatcgg cagcaatgcc aaaatcgggg cgggcagcgt
     1561 cgtggtttca gacgtgccgc cgtccatcac ggttgtcggc gtacccgcca aacccgtggc
     1621 gcgatcgctc aaaacaccgt cggcggatat ggatcaaaat atccagttta ccgaaatcga
     1681 ctttatgatt tgaatatccg caccaatgcc gtctgaaacg ccaaacgggc ttcagacgac
     1741 attgcccgct tcaagcctgc gccttgatat gccgccgatg tcggcacttg ccagtcggac
     1801 aaaatgcctt ttattcaata cacccgattc ccacgcaacc ggaaatatag tggattaaca
     1861 aaaatcagga caaggcgacg aagccgcaga cagtacggat agtacggaat cgattcactt
     1921 ggtgcttcag caccttagag aatcgttctc tttgagctaa ggcgagacaa cgccgtactg
     1981 gtttttgtta atccactata accgatttcc ttataaacgg gcggagtcgg gcgatgattc
     2041 ggacaaagcc tatttcaaaa gcaaaatcag cagcaaaaca accgcaatca cagccagcca
     2101 caggctttgc cgctgctgca ctttgaccaa atggatatag gcatcacgca tttcctgctg
     2161 gcggttttca tcaatcaacg cactgatttt gcgcggcaat gaagggatga tttgcgccca
     2221 gtcgggggct tcgtttttga ggttgcgcca aagggcttta gggccgacct gcccgttcat
     2281 ccatttcacc aaaaacggtt tggcggtttt ccacaagtcc aaatcgggat cgagctggcg
     2341 gcccaagcct tcgatgttga gcagcgtttt ttgcagcagc acaagctgcg gttggatttc
     2401 gacattgaag cggcggctga cttcaaacag gcgcatcagc accaagccga aggaaatctg
     2461 cgaaatcggt ttgttgaaca ccggttcgca cacggcgcgg acggcggctt ccaattcttc
     2521 cgcacgcgtg tcggcaggca cccagcccga ttcgatgtgg gcggtggcga cgcgccggta
     2581 atcgcggttg aaaaaggcga ggaaattgat ggcgagataa cgtttgtcgt aatcggtcag
     2641 cgtgccgacg atgccgaaat cgagggcgat gtagcggttg tcggcggcaa ccaaaatatt
     2701 gccggggtgc atatccgcgt ggaaaaagcc gtcgcggaag acctgcgtga aaaagatttc
     2761 cacgccgtaa tcggcgagtt tgtgcaaatc gatgccgtct gctttgagtt tggcgatgtc
     2821 ggcaaccggc gtgccgtcca tccattcgat tgtcagcacg tcgctggtgc agtagtcgta
     2881 aaacaccttg ggcacaatca gcatattgct gttttggaaa ttgcgcccga gctgtccggc
     2941 attggcggct tcgcgcatca aatccaactc gtcgtgcagg tatttgtcga actccgccac
     3001 cacttcgagc ggcttcagac gcctgccgtc tgaaaacaag cgttcgaccc aggccgcgcc
     3061 gaaacgcatc agcgacaaat cctgttcgat aaggggcaaa aggttggggc gcaaaacttt
     3121 aaccgccact cgttcgcccg aatgcaggcg ggctttgtgt acttgggcga tggacgcgct
     3181 ggcgatgggc tcggtttcaa attccgcata cagcttttcg atggactgac ccaacgattt
     3241 ttcgatttgt tcacgcgaaa gccgcgcgtc aaaaggcggc actttgtctt gcagctttgc
     3301 cagttcgacg gcgtaatcgt gcggaatcaa atcggggcgt gtggacaaaa cctgcccgaa
     3361 cttgatgaaa atcggcccca ggctttccaa ggcaaggcgc agacggacag caggcggttc
     3421 gtttttcaat ttggacgact gcggcatcat tttcagcaga gcgcatatcc aaccgctacc
     3481 catcagcgaa gcacacagac ctgccagccg atagcggtaa aaagtcccga caataaccgt
     3541 caggcgtttc aaccatttca tatataaaat cccgaataca gcatttatcg gacaatcaaa
     3601 aaatcttacc gagcagatag cccaaaaagg caatcagcag cccgaaaacc agcagtttga
     3661 tattgcggga cggcggttcg ggttgcgaag gtgcggaaat atcatcttcc cgcccgccgt
     3721 gaagttcaag atattcgggc atttccccat ccgcgccttc tccttcgccc tccccttcct
     3781 ccggcggcac gaccgtttcc aactccatat atctccgggt aataatatcc aaacccaaat
     3841 agctgaaata atcatgcaac tcttcccgtt tcgcccaagt gcatttttcc tcaaccagca
     3901 cttgaggttt ggaatcgagc gattcgatca atccttccgc cttttcctcg ccgagattct
     3961 ccttaaggca ctcccggata agcccgcgat ccacttgggg cgggtaagaa acatatacat
     4021 cgtacaaatc gtttcggtaa tctttactca tctcaatcat aagggaacaa atacgcctga
     4081 taccgttccg cccgttcctt tcgggaaacc cgattcatct tcaaacaatc ggattatatg
     4141 tgaattcatc ccgataatat attttctaaa agtaaaaatg ccgtctgaac gttcagacgg
     4201 catcacacaa accaagcggt catacggaaa acgacgatcc gcaaccgcag gttgtttccg
     4261 cattcggatt gcggatgacg aattgcgaac cctgcaaact ttccgtatag tcgatttccg
     4321 caccgaccag atattgatag ctcatcggat cgaccaaaaa gaccaaaccg tttttctcaa
     4381 tttcaaaatc gtcgtcgttt ttgatttcgt caaaagtaaa tccgtactgg aaacccgaac
     4441 agccgccgcc attgacaaaa acccgcaatt tcaaatcggg attgttttct tcggcaatca
     4501 aatcggcaac tttggcacag cagctgtcag taaaaataat agggctttca tccgacatgg
     4561 tgttattcct taatataata ttttctgcat tctctctcaa acccccttca aaggcaagca
     4621 ggcttcgcct ttgaaaaagg aattgttcca atagataagt ttgaccgcct gcaatttcaa
     4681 gccccaaact caaatcgtcc tgccgaacca ctcgacacgc ccgataatct ccacatcatc
     4741 ggtcaaatcg ttcaaattga tttcaaaagc aggataagcc tcgtttgcag aaatcacatt
     4801 gataatcccg cccggtacaa tctgtaaacg tttaaccagc agattttcat taatccgcaa
     4861 cacatacaga ccgtccctcg gcgtattttc accatgattg accaaaatcg aatcgccgtc
     4921 attcaaaacc ccctccatcg aatccccctt gacggaaatt acagacaggt ttttcgtatc
     4981 gcgggtaaca taattctcaa tccaatgccg tctgaacgcc attgtaaata ccggttcctc
     5041 atgatcgaca aactgcccgt atcccgcagc cgcccgaata tcatatctcg gcacgaagac
     5101 aaactcgtcc gtatcgactt cattgcccaa agtatcgtaa gcaagggatt tttttggggc
     5161 ttcatccgga aacggattac cctccccggt cagcagccaa tcgatactac accccttcaa
     5221 ctgcttgatt tttttcaatg tttcagactt tggcagaccg ccttcattcc atatcctgct
     5281 gaagcccgca atcgtcattt caatatcgga tgcgattttt gcctgcctcg cttcgctttt
     5341 ccataaaaaa accagtctgt ctttaaaggt ttccataaat atccttccac tccccttcat
     5401 tcaatcatct aaagtaaaca tgaaatatat tttaccttag ctatccaaaa aacggaacaa
     5461 ttatgataaa ctaaatttaa attatgattt ttcttactta atacataatt tttcagattt
     5521 ttccataggt ttaatcctga attgacagaa aaaggataga aaaatgaaga aaaccggcaa
     5581 atatcttatc tatactgcgg catttaccgc attctgcttt gccttccaag aaaaccgttc
     5641 tgaagccaaa cagcccgaca tcactttatc cgcatccctg tgcgaacaat tcaacatact
     5701 gaacgccaaa gatatggatg cagaacaagt ctccctttcc aaagaatgcg acatcatcga
     5761 gtcttcacac gactgggaaa aagagtacgg caacttgaac gaacaggaaa tgctcgccgg
     5821 catcgtctac gaataaacct gcctgccatt tgaaatatta tgcttgaatg cattggagcc
     5881 aaatgtatta aatcaaatat aaaaccaata tattcataaa gttatatact tatagccatg
     5941 ctgtagcttg aaacagcccg ccgcccgccc tatttacagc ccatcgggac aaaatgttcc
     6001 aacatatttc tcaaaataag caaaatcaaa taggattatc cacatggcaa aagtaatcgg
     6061 tatcgactta ggtacaacca actcttgttt ggccatttcc gaaaacggtc aaaccaaagt
     6121 tatcgaaaac gcagaaggcg cacgcaccac gccgtccgtt atcgcttatt tggacggcgg
     6181 cgaaatcctc gtcggcgcgc ctgccaaacg ccaagcggta accaacgcca aaaacactat
     6241 ttacgccgcc aaacgtttga tcggccacaa atttgaagac aaagaagtcc aacgcgacat
     6301 cgaatctatg cctttcgaaa tcatcaaagc caacaacggc gacgcatggg taaaagcaca
     6361 aggcaaagag ctgtctcctc ctcaaatttc cgcagaagtc ctgcgtaaaa tgaaagaagc
     6421 cgccgaagct tacttgggcg aaaaagtaac cgaagccgtg attaccgttc cggcctactt
     6481 caacgacagc caacgtcaag ccaccaaaga cgcaggccgt atcgccggtt tggacgtaaa
     6541 acgcatcatc aacgagccga ccgcagccgc tttggcattc ggtatggaca aaggcgacaa
     6601 caaagaccgc aaagtagccg tatatgactt gggcggcggt actttcgata tttccatcat
     6661 cgaaatcgcc aacctcgacg gcgacaaaca atttgaagta ttggctacca acggcgatac
     6721 tttcttgggc ggtgaagact tcgaccaacg cttgatcgac tacatcattg acgagtttaa
     6781 aaaagaacaa ggcattgatt tgaaacaaga cgtcatggct ctgcaacgtc tgaaagaagc
     6841 tgccgaaaaa gccaaaatcg aattgtccag cggccagcaa accgaaatca acctgccgta
     6901 catcaccatg gacgcaaccg gcccgaaaca cttggcgatg aaaattaccc gcgccaaatt
     6961 cgaaagccta gttgaagacc tgattgcccg ctctatcgag ccttgccgca ccgcattgaa
     7021 agatgccggt ctgagcactg gcgacatcga cgacgtgatt ttggtcggtg gtcaaagccg
     7081 tatgccgaaa gtacaagaag ccgttaaaga cttcttcggc aaagagccgc gcaaagacgt
     7141 gaaccctgac gaagctgtcg ccgtaggcgc agcgattcaa ggcgaagtgt taagcggcgg
     7201 ccgcagcgac gtattgctgc tggacgtaac ccctctgtcc ttgggtatcg aaaccatggg
     7261 cggcgtgatg accaagctga ttcaaaagaa caccaccatc ccgaccaaag cgtcgcaagt
     7321 gttctctacc gccgaagaca accaaagcgc agtaaccatc cacgtactgc aaggcgaacg
     7381 cgaacgcgct tctgccaaca aatctttggg tcagttcaac ttgggcgaca tcgcacccgc
     7441 accgcgcggt atgccgcaaa tcgaagtcac cttcgatatt gacgccaacg gtatcctgca
     7501 cgtttccgcc aaagacaaag gcactggcaa agcagccaac atcaccatcc aaggttcttc
     7561 aggtttgagc gaagaagaaa tcgaacgcat ggtgaaagat gccgaagcca atgccgagga
     7621 agataaaaaa ctgactgaat tggtcgcttc ccgcaaccaa gccgaagccc tgattcactc
     7681 tgtgaaaaaa tctttggccg actacggcga caaactcgac gcagccgaga aagaaaaaat
     7741 cgaagccgcg ctgaaagaag ccgaagaagc agttaaaggc gacgacaaag ccgctatcga
     7801 tgccaaaacc gaggcgctgg gcgcagccag ccaaaaactg ggggaaatgg tttacgctca
     7861 agcacaagct gaagcccaag caggcgaaag cgaacaagcc aatgcttctg caaagaaaga
     7921 cgatgatgtc gtagatgccg actttgaaga agtaaaagac gacaaaaaat aattagggcg
     7981 tgtccctaat ttgaataagc ccaaaagagc cctattttaa ccctatattc caac
//
LOCUS       DAXHTR010000099         7993 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.099, whole genome shotgun sequence.
ACCESSION   DAXHTR010000099 DAXHTR010000000
VERSION     DAXHTR010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7993
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.099"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(263..2953)
                     /gene="glnE"
                     /locus_tag="ACFNML_001500"
     CDS             complement(263..2953)
                     /gene="glnE"
                     /locus_tag="ACFNML_001500"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFC6392856.1"
                     /translation="MSDNRLDTARRHSLFLARQLDNGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDKIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQISS
                     EVSKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLKKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQQQLLAESMGFDSYAAFSDDL
                     NAHRTKVNQLFNEILSEPEEQTLDTSEWQWAWQEKPDEEGRRCRLKAHGFDAETVAAR
                     LDQIRHGHKYRHLSAHAQPRFDAVVPLFVQAAAEQSNPTDTLMRLLDFLENISRRSAY
                     LAFLNEHPQTLAQLAQIMGQSSWVAAYLNKYPILLDELISAQLLDTAFDWQALAAALS
                     DDLKACGGDTEAQMDTLRRFQHTQVFRLAVQDLAGLWTVESLSDQLSALADTILAAAL
                     LCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPDAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLAHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEMLTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARQYPQLLDNYGNIALLNIAADCSLIDKNLAEQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVTGELLAHYGNVRKLWRDVFGEEAATA"
     gene            complement(3151..3486)
                     /locus_tag="ACFNML_001501"
     CDS             complement(3151..3486)
                     /locus_tag="ACFNML_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392857.1"
                     /translation="MPSEALSGLQTAFCFPTVYKLSLFLDRNNDLIYLKRLEDMIMGF
                     WNGVAKAAKAVGEGMIEAGNEHKALKMEYAEKSSEELHEIVKSDGFFKNSTREKSAAY
                     AILKERGEV"
     gene            complement(3500..3973)
                     /locus_tag="ACFNML_001502"
     CDS             complement(3500..3973)
                     /locus_tag="ACFNML_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HFC6392858.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFEDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4026..4307)
                     /locus_tag="ACFNML_001503"
     CDS             complement(4026..4307)
                     /locus_tag="ACFNML_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392859.1"
                     /translation="MENGQRNNRFPLEKRIFYLEHSGRYLMICALSDYSQNKHTVVMA
                     NFIYPDEKTDWRNLDDLFNELVLEELQASFMDWHPTVEEAISRHLEDFS"
     gene            complement(4394..4717)
                     /locus_tag="ACFNML_001504"
     CDS             complement(4394..4717)
                     /locus_tag="ACFNML_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392860.1"
                     /translation="MFKDELNEFIRLISDPESELDEWYLSDFKDEHIWEMQSYEAFSC
                     LREAVPYLFAYPRYGYELLEIISALKETSDTTELFYEPGIVPLLIDLYKEDSYLVNMV
                     KRIFK"
     gene            complement(4856..5035)
                     /locus_tag="ACFNML_001505"
     CDS             complement(4856..5035)
                     /locus_tag="ACFNML_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392861.1"
                     /translation="MPSERTFCRSDGIFISFSCLPPFENPLRAALFETKQTPAANRTI
                     LIRSQSKSGGKLLNI"
     gene            complement(5051..6058)
                     /locus_tag="ACFNML_001506"
     CDS             complement(5051..6058)
                     /locus_tag="ACFNML_001506"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFC6392862.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALNMVYKLGLIPVTGNRTKPV
                     KLMGLDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPNPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDAMIQNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     Q"
     gene            6290..6526
                     /gene="acpP"
                     /locus_tag="ACFNML_001507"
     CDS             6290..6526
                     /gene="acpP"
                     /locus_tag="ACFNML_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFC6392863.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            6682..7929
                     /gene="fabF"
                     /locus_tag="ACFNML_001508"
     CDS             6682..7929
                     /gene="fabF"
                     /locus_tag="ACFNML_001508"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFC6392864.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
BASE COUNT         1846 a         2129 c         2064 g         1954 t
ORIGIN      
        1 tagaatgcgc tattttaatg gaaacggcgg ggttttaaac ggttcttatt tgtttgtttt
       61 gatttttgct tgggtaaaaa atcccatcat tcccacaaaa acagaagcct gaaatcccgt
      121 cattcccgcg aaagaggaat ccggtttttg ggtttcagcc attcccgata aatcgcttta
      181 gctctgccgc aaccttaaaa cggcacggta cgaaaatacc gtctgaaacc cagattgtca
      241 ggcttcagac ggcatttttt gttcaggcgg ttgccgcttc ttcgccgaac acgtcccgcc
      301 acaatttcct gacattgccg taatgtgcca acagttcgcc ggttacttcg gtttttgccg
      361 cgtcgcgcag tttggtgttg tgctgctgcc ggcggtagaa gcggtaggcg gtgcggcttt
      421 gttcggcgag gtttttgtcg ataaggctgc agtcggcggc gatgtttaag agggcgatgt
      481 tgccgtagtt gtccaagagt tgcggatact ggcgggcatg ggcaagtatc agatattgga
      541 cgataaattc gacatcgacc acgccaccgc gcgcgtattt gacgttgctg tcggcaggcg
      601 gatgggtggg gaacattttt tcgcgcattt cgataatttc gcctgctaag gcggtttggt
      661 cgcgttcggc agtcagcatt tcggtgcgga tgcggtcgaa ggccgtctga atttcgggtg
      721 tgccgcagat gaagcgggcg cgggtgaggg attggtgttc ccacgtccag gcgttttcgc
      781 gctggtattt ttcaaaggcg gcgatgctgt gggcgaggaa accggcgtcg ccattagggc
      841 gcaggcgcag gtcggtttcg tagaggctgc ctgcgccggt ggcggcggaa agccagttgg
      901 tcaggcgacg ggcgaggcgg ctgtacacgt cgcctgcgtc ggggtgggga tcgtcgtaga
      961 gatagacgag gtcgaggtcg gaggcgtagc cgagttcttt gccgccgagt ttgccgtaac
     1021 caacgacggc gaattgcggg gtgtcgcggt gttttttggg catgtccgcc cacgcgcaca
     1081 gcagggcggc ggcgaggatg gtgtcggcga gggcggagag ttggtcggag agggattcta
     1141 ccgtccacag tccggcgagg tcttggacgg cgagacggaa gacttgggtg tgctggaagc
     1201 ggcgcagggt gtccatttgc gcttcagtat cgccgccgca ggctttgagg tcgtctgaaa
     1261 gggcggcggc gagtgcctgc caatcaaacg cggtatccaa aagctgcgcg ctgatgagtt
     1321 cgtccaacaa aatcggatat ttgttcagat acgccgccac ccaagaactt tggcccataa
     1381 tctgcgccag ttgcgccaag gtttgcggat gttcgttgag gaaggcgaga taggcggatc
     1441 ggcggctgat gttttcgaga aaatccaaca gccgcatcaa tgtatcggtc gggttgcttt
     1501 gctctgccgc cgcctgtacg aacagcggca caaccgcatc gaaacgcggc tgggcgtgtg
     1561 cggaaagatg gcggtattta tggccgtggc ggatttggtc gagccttgcg gcgacggttt
     1621 cggcatcgaa cccgtgcgcc ttcagacggc atcgccgccc ttcttcgtcg ggcttttcct
     1681 gccatgccca ttgccattcg ctggtgtcta gggtttgctc ttcgggttcg ctcaaaattt
     1741 cgttgaacaa ctgattgact ttggtgcggt gggcattgag gtcgtctgaa aaagcggcgt
     1801 agctgtcgaa acccatactt tcggcaagca gttgctgctg ttcgggcgaa gccggcaggg
     1861 tttgggtttg ccggtcatcc cagtattgca ggcggtgttc gacatcgcgc aggaatcggt
     1921 aggcggcaag cagggtttcg acgttttcag acggcatgat cccggtttcg gcaagtttct
     1981 tcagcgtttc ctgcgtgcct ttcagttgca gcgcacgcat ttggccgccg cgtatcatct
     2041 ggaaaatctg ggcgataaat tcgacttcgc ggatgccgcc cgcgccgagc ttgatgttgt
     2101 ccgccatgcc ttttttgctg acttcgctgc tgatttggcg gtgcagctta cgcatcgcct
     2161 catacgcgcc gtaatccaga tatttgcgga acacgaaggg gcgcaccagc gatttgattc
     2221 cgttcggata cggcgtaacc acgcggcctt tgcaccacgc gtagcgttcc cattcgcgcc
     2281 cctgcgtaat caggtattgc tccagcgcgg tttcgctcaa caccaacgcg cccgaatcac
     2341 cgtccggccg cagccgcata tcgacgcgga acacctgccc gtcggcggtg atgtcgttca
     2401 acagcgcaat cagtttctgc ccgactttgg tgaaaaactc ctgattgccg cgttcgcgcc
     2461 tgccgtcggt gtcgcccgat tcgggataga cgaaaatcaa atcgatgtcg gaagacacgt
     2521 tcaactcata gccgcccgcc ttgcccatcg ccaccacgct caaatgctgc ggcgatttgg
     2581 tataacgccc gatcggcgtg ccgtacatgt cccgataata ggcgtaggca aaatccagcg
     2641 cggtattgac ggcaaaatcg gcaaacagcg taatcgtgcg ggttacttcg ttcaaatcgc
     2701 tgatgcggtt tatatcgcgc acgataatct gcgacaccac ataacggcgc aacccgcgca
     2761 actgccgcgc caattcttcc tcgttttctt ccgcacggat tttgtcccag tcggcaaagg
     2821 cttggaaatc cgcttcggtc aaaaccttgt ccagcatagg caggaaaatt tcgggcttga
     2881 gtttgccgtt gtcgagctgg cgggcgagga agagggaatg gcggcgggcg gtgtcgaggc
     2941 ggttgtcgga catttcggat tccgtttgaa aaaatggcag gaagatgatg gcggattcac
     3001 ttcaaatcag gacagggcga ggggatgccg taccggttta aagtcaagcc actatatcat
     3061 agcaaccttc gatgccgtct gaaaccttgc cccgacatcc agacggatgc ctgccgataa
     3121 aaaacagcgg caaatgccgc cgtttcctgt tcacacctcg ccacgctctt ttaaaatagc
     3181 ataagccgca cttttctccc gtgtggaatt tttaaaaaaa ccatcactct tgacgatttc
     3241 atgcagctcc tcacttgatt tctccgcata ttccattttc aacgccttat gctcattgcc
     3301 ggcttcaatc attccctctc ccactgcttt tgctgctttt gccacaccat tccaaaaacc
     3361 cataatcatg tcctccaatc gtttcaaata aataaggtcg ttgtttctgt caagaaacaa
     3421 cgacagttta taaactgtcg gaaaacaaaa tgccgtctga aggccggata atgcttcaga
     3481 cggcatcgga ttgccggttt taaccctgcc ctccgccgtt gttcaaatag tgttcccagt
     3541 gagaatataa ccgggcaata ttttcttcat aggtgtgggc agaaggaata taaccgtctt
     3601 tgggcggcat atacctgaca aatgattcta tttcttccgg ataatcaaaa tctgcataaa
     3661 ttttttcaac ttcccccaaa ggatcctcaa attcttcttt tctatggaat acatcgctta
     3721 agaggacgta cagccaattt ttaggggaat aatccctcat ttccgaagcc aaatcttcca
     3781 gaacaggctt taattcaaga atattggatt tgttgattaa agatagtttg aacacacgtt
     3841 catcatgatc tgaaagcgtc atttttcggg cataagctgc cacatcagcc caacccaagt
     3901 atttattccc atacccccac agaatatcct tccaagaaag acggactttt tgcgcggtta
     3961 aatcaaggtt cattttttac tccgattgcc tgccccgtca ttccgcccct tccgattgac
     4021 gcctgttacg aaaagtcttc caaatgacgg ctgattgcct cttcaacagt cggatgccaa
     4081 tccataaatg aagcctgcaa ttcctctaaa accaactcat tgaataaatc atccaaattc
     4141 cgccaatccg ttttttcatc cggatagatg aaatttgcca taacgacagt atgtttgttt
     4201 tgggaataat ccgataaggc gcaaatcatc agataccgcc ccgaatgctc cagataaaaa
     4261 attcgttttt ccaaaggaaa ccggttattc cgttgcccgt tttccatttt tcatcctttt
     4321 taacgtatcg gcttccgcgt ccttcaaatt gagttacctg ccttccgcca agcaaacgcc
     4381 cgcgcccctt tcatcatttg aatatccgtt tgaccatatt taccagataa ctatcttctt
     4441 tatataaatc aatcagcaaa ggcacgatac ccggctcata aaataattct gtcgtatcag
     4501 atgtttcttt taacgcggaa attatctcca acagttcata cccgtatcgg ggataagcaa
     4561 acaaataggg gactgcttcc cgcaaacatg aaaatgcttc gtaactttgc atttcccata
     4621 tatgttcatc tttaaaatca ctcaaatacc attcatccaa ttcactttcc ggatcggata
     4681 tcaatcggat aaattcattt agttcgtcct taaacatatc aaatccttaa atataaaatc
     4741 ttttgattat ccgccgtatc cttattttcc acatcatgaa tttcaacgcc gttcatattt
     4801 tgcgttttct caaaccgtcc accacggcat aaaattattt tttcatatca attatttata
     4861 tgtttaacaa cttaccgccg gacttgcttt ggctgcgtat caagattgtc cggttcgcgg
     4921 cgggcgtttg ctttgtttca aacagggcgg cgcgtaaggg attttcaaag ggcggcaggc
     4981 aggaaaagga aataaaaatg ccgtctgaac ggcaaaacgt gcgttcagac ggcattttgt
     5041 gcgggacgcg tcattgcgcc aaagccttca aacattcttt gaccaatgcc ggacctttgt
     5101 atatcaatcc gctgtacacc tggacggcag ttgcgcccaa gcggattttc tctgccgcgt
     5161 ccctgccttc cataatgccg cctacgccga taatcggcag cttgccgtcg atgtggtctg
     5221 ccaacagctt caacacccga ttactttttt catgaacggg cagcccgctc aaaccgccct
     5281 gctcgcctgc gagcggatgg ctgccgagac ttgatttgtc gatggtggta ttggtagcga
     5341 tgatgccgtc catttcgacg gatttgacaa cgtgggcgat gtcttcgatt tgtgcttcat
     5401 ccaaatcggg ggcgattttg acggcgagcg ggacgtattt cccgtgtaca gaggcaagct
     5461 gtgcctgttt gtttttcaaa gcctcaagca atgcgctcaa ctcgtcgcca ccttgcagcg
     5521 cgcggaggtt tttagtgttg ggcgaggaaa tattgacggt aatgtaactt gcgtgtgcgt
     5581 aggctttttc gaggcagatt aaataatcat cggcagcgtt ttcaatcggt gttacagcgt
     5641 ttttgccgat gttgatgccc aatacgccgc tgaatttact tttttcgatg ttttgaatca
     5701 tggcatcgat gccgtggttg ttgaagccca tgcgattgat gatgccttga tattcgggaa
     5761 cgcggaagag gcgcggctgc gggttgccgg gctgcgggtt gggcgttacc gtgccgattt
     5821 cgatgaaacc gaagccgagc gcgcccaatg cgtctatata ttcgccgttt ttatcgagtc
     5881 cggcggcaag cccgacaggg ttgggcaaat ccaaacccat cagttttaca ggtttggtac
     5941 ggttgccggt tacgggaatc agacccaatt tataaaccat gttgagcgcg tcgagcgtga
     6001 agtggtgggc tttctcggca tcgagtgcaa acaggatgcg gcgggcaagt ggatacatga
     6061 cagggctttc tgcttcagat ggaaatgccg tcattttaac cgaaatcccg tcaaacatac
     6121 ggcacacagg tcaaacttga acgacaaaac ccgccgcacc caaaaatgta caaatcggca
     6181 aatattgata taatagtcat tagatagaaa actctattaa aagtcggtac aataccaccg
     6241 atttatttcc cttaaacaac ctgccgcaag gcataaagga acgactgata tgtcaaacat
     6301 cgaacaacaa gttaaaaaaa ttgttgctga acaactgggc gtaaacgaag ccgacgtgaa
     6361 aaacgaatct tctttccaag acgacttggg cgcggattct ttggacaccg tggagctggt
     6421 tatggctttg gaagaagcat tcggctgcga aatccccgac gaagatgccg aaaaaatcac
     6481 caccgtccaa ctggctatcg actacatcaa tgcccacaac ggctaaccgg tcgtcgcccg
     6541 acacaacagc ctctgctgca ccgcgcaata gaggcttttc ccttatatgg caaactgttt
     6601 gaacccctgc cgcaaattac agacggcatc tgcacgacag ccgcatatgc ccccattccg
     6661 acaaccaaca gcgaggttat catgagtcag agaagagtag tcattacagg cttaggtcag
     6721 gtttcccctg tcggcaacac tgtcgcagag gcttgggaca ccctgctcgc cggcaaaagc
     6781 ggcatcggcg cgattacccg ctttgacgca tccgacatca acagccgtgt cgccggcgaa
     6841 gtgcgcggtt tcgacatcgg acaatacatc agcgcgaaag aagcgcgccg gatggacgta
     6901 ttcatccact acggcattgc cgccgcattg caggcaatcg ccgattcggg cttggacgat
     6961 gtggaaaacc tcgacaaaga ccgcatcggc gtgaacatcg gttccggcat cggcggactg
     7021 cccagcatcg aggtcaccgg caaagccgta atcgaaggcg gcgcgcgcaa aatcaaccct
     7081 ttctttatcc ccggttcgct gatcaacctg atttccggac acgttaccat cctcaaaggc
     7141 taccgcggcc cgagctacgg gatggtttcc gcctgtacca ccggcgcgca cgccatcggc
     7201 aattccgccc gactgatcaa atacggcgac gcggacataa tggttgccgg cggcgcggaa
     7261 ggcgcaatca gcactttggg cgtgggcggt tttgccgcga tgaaagccct ctccacccgc
     7321 aacgacgacc ccgccaccgc ttcccgtccg tgggacaaag gccgcgacgg cttcgttatc
     7381 ggcgaaggcg cgggcatatt ggtgttggaa gaattggaac acgccaaaaa acgcggcgcg
     7441 aaaatctacg ccgaaatcgt cggcttcggc atgagttccg atgcttacca tatcaccgcg
     7501 ccgaacgaag aaggccccgc ccttgccgtt acccgcgcgc tgaaagatgc cggcatcaat
     7561 cccgaagacg tggattacgt caacgcgcac ggcacgtcca cccccttggg cgatgccaac
     7621 gaaaccaaag ccctcaaacg cgcgttcggc gaacacgcct acaaaaccgt cgtcagctcg
     7681 accaaatcca tgaccggcca cctgctcggc gcggcgggcg gcgtggaggc cgtgtacagc
     7741 attttggcga tacacgacgg caaaatcccg ccgaccatca acatttttga acaagacgtt
     7801 gaagccggct gcgatttgga ctactgcgcc aacgaagcgc gcgacgcgga aatcgacgtt
     7861 gccatttcca actccttcgg cttcggcggc accaacggca cgctggtctt caaacgcttc
     7921 aaaggctgat tccgcaaagc cgccgccgac atcgaaatgc cgtctgaaac cgtttcagac
     7981 ggcattttta tag
//
LOCUS       DAXHTR010000100         7923 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.100, whole genome shotgun sequence.
ACCESSION   DAXHTR010000100 DAXHTR010000000
VERSION     DAXHTR010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.100"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            331..1644
                     /locus_tag="ACFNML_001509"
     CDS             331..1644
                     /locus_tag="ACFNML_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HFC6392865.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILMPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1865..2022
                     /locus_tag="ACFNML_001510"
                     /pseudo
     CDS             1865..2022
                     /locus_tag="ACFNML_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003824008.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            2035..3285
                     /locus_tag="ACFNML_001511"
     CDS             2035..3285
                     /locus_tag="ACFNML_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6392866.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTERGRIAV
                     MQESEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQSAVLQQSIRRNKHLLEQLLALARSQSDETPLAKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVGGDVEIFADETEIYTLVKTFVDNAIRYTPSEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERVRVLDPFYRILGTEQQGAGLGLSIADTLAKKYGGYL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3320..4813)
                     /gene="rng"
                     /locus_tag="ACFNML_001512"
     CDS             complement(3320..4813)
                     /gene="rng"
                     /locus_tag="ACFNML_001512"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFC6392867.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEKTQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNIEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            5026..5283
                     /gene="grxC"
                     /locus_tag="ACFNML_001513"
     CDS             5026..5283
                     /gene="grxC"
                     /locus_tag="ACFNML_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFC6392868.1"
                     /translation="MQTVTMYTGPFCPYCAMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5304..5747
                     /gene="secB"
                     /locus_tag="ACFNML_001514"
     CDS             5304..5747
                     /gene="secB"
                     /locus_tag="ACFNML_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFC6392869.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDVQLLLGVACP
                     NILFPYAREAVSSTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5833..7875
                     /gene="recG"
                     /locus_tag="ACFNML_001515"
     CDS             5833..7875
                     /gene="recG"
                     /locus_tag="ACFNML_001515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFC6392870.1"
                     /translation="MMSPETQKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLQHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQARETAPMLIEQNPEIVEAHLARWLSGREGYLGV"
BASE COUNT         1658 a         2045 c         2353 g         1867 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta taaaatccca cgtgtacggc acgccccgcg
       61 ctccgcaatg ataggggcgg tttcgtttga atcggcgtac cgggatgaat gcccccgcgc
      121 cgtcccgata ccggtttgcc ggttgttccg tatcggttgc cgccgtgaaa tgacggacgg
      181 atacaggaat ggcggcaggc gtaaatggga ggaaatgacc gattgacttg tttcaaaata
      241 aaaaagtaga atgcaaggct tatcccgcgt ctgtgggcgg ttttgcctgc acggtcggtt
      301 ttctgtttaa aataaaataa aggagaaatg atgctgacgt ttatcggact gctgattatc
      361 ggggtcatcg tatggttgct gctgacggaa aaagtgtcgc ccatcatcgc attaatcttg
      421 atgccgctgt ttggggcgtt gctggcgggg tttgatgtat cccaattaaa agaattttat
      481 tcgggcggca ccaaatcggt gatgcagatt gtgattatgt ttatgttttc cattttgttt
      541 tttggaatca tgaacgatgt ggggctgttc cgtccgatga taggcggttt gattaagctg
      601 actcggggta atatcgtggc agtgagtgtg gggacggtct tggtgtcggt ggtggcgcag
      661 ttggacgggg cgggtgcgac gacgttttta ttggtcgtcc ccgccctttt gccgctttac
      721 aagcgtctgc atatgaatcc ttacctgctg tttttgctgc tgacttccag tgcgggattg
      781 attaaccttc tgccgtgggg cgggccgact gggcgggttg caagcgtgtt gggcgcagat
      841 gtgggcgaat tgtataaacc tttgttgacg gtgcaaatta tcggtgtagt gtttatcctt
      901 gcgctgtccc tgcttttggg tgtgcgtgaa aaaaggcgga ttgtccggga gttgggcgcg
      961 ttgcccgccg tggcggattt gataaagccg gtgcctttgt cggaagaaga acaaaaattg
     1021 gcgcgtccga aactgttttg gtggaatgtc ctgctgtttt tggcggcgat gagcctgctt
     1081 ttttcgggta tcttcccgcc gggttatgta tttatgctgg ctgcaacggc ggcgttgctt
     1141 ttgaattacc gcagcccgca ggaacagatg gagcggattt atgcccacgc cggcggcgcg
     1201 gtgatgatgg cgtccattat tttggcggca ggtacgtttt tggggatttt gaagggtgcg
     1261 gggatgttgg acgcgatttc caaagacatt gtgcatatcc tgccggacgc gctgctgcct
     1321 tatctgcata ttgccatcgg tgtgttgggc attccgcttg agttggtttt gagtacggac
     1381 gcttattatt tcggactgtt tccgattgtg gagcagatta cctcgcaggc gggcgtggcg
     1441 cccgaagcag caggttatgc gatgttgatc ggcagtatcg tcggcacttt tgttacgccg
     1501 ctttcgccgg ctttgtggat gggcttgggt ttggcgaaat tgtcgatggg caaacacatc
     1561 cgttattcgt ttttttgggc gtggggtttg tcgctggcga tattggccag ttcgatagcg
     1621 gcaggaatcg tgcctctgcc gtaaacggcg aagccgtctg aagcccgaat cgttcctgtg
     1681 gtctgtcgca ggctgcctgc ctgcccgtgt gttgcaatcg cgccccaatg ccgtctgaag
     1741 gcagttgccg gcgcggtttt tttgtgctgc agttttgaaa aataccggca agcgtaaagg
     1801 ccgctaaatt gcggctaatt ttggggtgtt agaataggca cggtttgtct gaaagggaaa
     1861 gttgatgcgt gttttgctgg tggaagacga tgcgatgatt gcggaggcgg tgtcggcaag
     1921 tttgaaagac ggcggctatg cggtggattg ggtcaaaaac ggcgcgcggc tgccgctgcc
     1981 cagccttatg acttgatgct gctggatttg ggtttgccgg ggcggacggt ttggatgttt
     2041 gttccgctgg caatgcttgc aggtatgttt tcctactacg aaaccttcca cgaaacggaa
     2101 gcgttgcagg acgacctgct ccgtcaggcg gcattgtacg tcgcccccga ttccaaaccc
     2161 gaaactttgc ccgagggcga cggcgatacg cgtattttgg tacagatgcc gcagcaggaa
     2221 gaccctgttg tcagcctgcc tgcgcatctg gcggacggtc tgcacacgct tcaggcggac
     2281 ggggacgacg attattaccg cgtctatatc cgcacgacgg agcgggggcg gattgccgtg
     2341 atgcaggaaa gcgaataccg ggaagatttg gcggcggatg cggcacggca aagcgtgttg
     2401 cccctgctgg cggcactgcc gctgatgata ctgctgaccg tgtggattac gcacaaagcc
     2461 atgcgccccg tccgcaaatt gtcgcaaagt ctcgaacaac gccgaatcaa tgacctgtct
     2521 gccttgagtg tggacaatat tcccagtgaa atcagagggt tcgtaaccgc catcaacttg
     2581 cttttgaaac gtgcggatga agacatccgc caccgtcagc ggtttgtcgc cgacgcggca
     2641 cacgaattgc gtacgccgat gacagccctt tcccttcagg cggaacggct caacaatatg
     2701 tctctgccac ccgatgcggc gcggcagtct gccgttttgc agcaaagcat acggcgcaac
     2761 aaacacctgc tcgagcagct tttggcactg gcgcgttcgc agtcggacga aacccctttg
     2821 gcgaaaacga cattcggtct gcaaagccgt ttccgccaag tgttgcagga actgatgccg
     2881 cttgccttgg aaaaacgtca ggacattggt gtggcggtcg gaggtgatgt cgaaatattt
     2941 gccgatgaaa ctgaaatcta taccttggtt aaaacgtttg ttgataacgc aatacgttat
     3001 accccgtccg aaggcaggat agatttgggt ttcacagacg aagggaaata tcttgccgtg
     3061 tgggtggaag ataacgggaa cggcattccg gaatccgaac gcgtccgcgt cctcgatccg
     3121 ttttaccgta ttttgggaac ggagcagcag ggggcggggc tggggctgtc gattgccgac
     3181 acgctggcca aaaaatacgg cggatatttg gaactgaccg acagccgacg tttcggacac
     3241 gggctgttga tacgcgcgct gttggacaag gaaaccctga aatagacaga tgccgtctga
     3301 aggcttcaga cggcattttt tacatcagca caatatcgta ctgttcctgc gtgtaagcgg
     3361 tttcgaccgc caaagaaatc ggtttgccga tgaaatctat cagcattgcc aaggattgcg
     3421 attcttcgtc caaaaacaaa tcgatgacgt tgggggcggc gaggatgcgg aaactttcgg
     3481 catcgtaacg gcgcgcttcg cggacgattt cgcgctggat ttcgtagcac acggtttgcg
     3541 gcgttttcag gcggcccctg ccttggcagg aagggcaggg ttcgcagagg acttggttta
     3601 agttttcgcg cgagcgtttg cgcgtcagct cgaccagccc caggctggta aaaccgtgca
     3661 gggtaacgcg ggtacggtcg aaggcgaggg ctttggcaag ctcctgcaac acggcttcgc
     3721 ggtggctttc ctgcgccata tcgatgaagt cgatgatgat gatgccgcca aggttgcgca
     3781 gccttaattc gcgggcgatg gtgtggcagg cttcgaggtt ggtgcggaag atggtttcgt
     3841 cgaaattgcg cgcgccgacg aagccgccgg tgttgacatc gatggtggtc atggcttcgg
     3901 tggactcgat aatcaggtag ctgccgaagt tgaggttgac gcgcggttgc agggcgcggc
     3961 tgatttcctg ttcgatgttg tgggtttcaa acagcgggcg ttcgcctttg aacaattcta
     4021 tcctgcccaa tgcgccgtgg acgtattgtt cggcaaaacg cgtcatgcgc ccgtggttta
     4081 cggtggaatc gacgaggatt ttttgcgtgt cgcagccgac catatcgcgc aatacgcgca
     4141 aactcaaggg caaatcctga taaagcaggg tttccggcgg ccggattttc gcctgttctt
     4201 ggatgtgttc ccacactttg gtcaggtagt cgatgtcgga ctggagctgt tcgtcggtgg
     4261 cgttttcggc gttggtgcgg atgatgtagc ctcggcaggc attttccggc aggagcttgt
     4321 cgaggcgttc gcgcaggctg ctgcgttcgg catcgtcttc gatgcgttgg gacacgccga
     4381 tgtggtcttc ttgcggaaga tggacgagga aacgccccgc cagcgagatt tgggtggaaa
     4441 gccgcgcgcc tttggtgttg atcgggtctt tgatgacctg caccaaaaca gactgccctt
     4501 caaacagcat atgttcgatg cgctgggttt tttcggggtt gcggcgttgt tcgaggacat
     4561 cgacgatgtg taaaaacgcc gcgcgttcca agccgatgtc gataaacgcg ctctgcatcc
     4621 cgggcagcac gcggcgcacc acgcccagat agatattgcc gaccaggctg tgttcgctgt
     4681 tgcgctcgat gtgcagctcg cagatattgt tttcctccaa caccgctacg cgcgtttcct
     4741 gcggcgtgat gttgaccaat atcgtttcgg gcgggcgcgc gatgtctttg gggatgggga
     4801 gtcctgacaa catggttttt cctgaaaatg taataaaaat attttgcatc cgttccgccg
     4861 cgcagcgcgg gtgcaaaaat atttttcaca atgtctatca tactttaaaa aagaaacttt
     4921 gacacattcc gcacatgccg tttgaacgtg catctgttcc gcacttgcca aacaggggaa
     4981 atgcccctat attgattatc attgcaaaac tttcggaaac ccaatatgca gaccgttacc
     5041 atgtacacag gtccgttttg tccctactgc gcgatggcga aaaggctgct gcacgcggca
     5101 ggtgtcggac atatcgacga aatccgtgtc gatgcaagcc ccgaagcctt tgccgaaatg
     5161 cagcagcttt cgggacagcg cagcgtgccg cagattttca tcggcgaaac gcacgtcggc
     5221 ggatttaccg acctctaccg cctccagcag gaaggcgggc tggacggact gctgaaccct
     5281 taacccccaa ctaggaaaac aaaatgagcg aagaactgca acccgtattc agcatcgagc
     5341 gactgtatgt caaagacttg tctttggaag tgccgcacgc gccgcaaatc tttttggaac
     5401 agggcgagcc cgaagtggat atgcgcgttt ccaccggcag ccaaaagctg gaagacggct
     5461 actacaacgt ggatgtaacc gttaccgtaa ccgccaaatt ggataacgag cgcacgatgt
     5521 ttttgaacga agtaacccaa agcggcatct tccgtttgga aaacatcccc gaagaagatg
     5581 tgcagctgct gctgggcgtg gcgtgtccga acatcctctt cccttacgcg cgcgaagccg
     5641 tttccagtac ggtaacgcgc gccggcttcc cgcccgtcct gctcgcgccg attaattttg
     5701 aggcgattta ccaacaacag caggaagccg aagccgccgg ggcttgattc ctgcctgccg
     5761 atgccgtctg aatccgtttc agacggtatt tttgttttcg gataaaatag gtgcgttcaa
     5821 aggaatctgc ctatgatgtc gcccgaaacc cagaaacagc tcaaaatcac cgatgtttcc
     5881 gccaagaagc tcgacaaact caacctccat accgcgtggg atttggtgtt gcacctgccg
     5941 ctgcgttacg aggacgagac gcacattatg ccgattaagg acgcgccgat tggcgtgccg
     6001 tgccaggtcg agggcgaggt tatccatcag gaagtaacgt tcaaaccgcg caagcagttg
     6061 attgtccaaa tcgccgacgg ttccggcagc gtcctttttc tgcgcttcat ccacttttac
     6121 gccagccatc agaaacagac ggcggtcggc aaacgcatcc gcgccgtggg cgaaatcaaa
     6181 cacggctttt acggcgacga gatgattcat cccaaaatcc gcgatgccga gggcggcggt
     6241 ttggcggaaa gcctcacgcc ggtttacccg accgtaaacg gtttgaacca gcccactttg
     6301 cgccgtatta ttcagacggc gttggacgtt acgccgctgc acgacacgct gcccgatgcc
     6361 ctgctgtgcc gtctgaagct gcaacacctc gccgaaagcc tgcgtctttt gcattcgccg
     6421 ccgccgagtt tcacgattca tcagctttca gacggcacgc tgcctgcatg gcaacggctc
     6481 aaattcgatg aacttttggc gcaacagctt tcgatgcgct tggcgcgaca gaagcgtatc
     6541 ggcggcacgg cggcggcatt gggcggcgac ggcacattga cccaagccct gcgccaagcc
     6601 ctgccgtttg ccctgaccga tgcgcaagaa aaagttgttt ccgaaatctg ccgcgatatg
     6661 gcgcaaacct acccgatgca ccgcctgctg cagggcgatg tcggcagcgg caaaaccatt
     6721 gtggctgctt tgtctgcttt gacggctatc gaatccggtg cgcaggtggc tgtaatggcg
     6781 cccactgaaa tccttgccga acagcatttt attaaattta aacaatggct cgaacctttg
     6841 ggcattgaag ttgtccgcct ttttggcagt ttgcgtaaaa aagccaaaga cgaagccaaa
     6901 gccaaactgg ctgacggcag cgtcaaaatc gccgtcggca cgcacgccct gttttcagac
     6961 ggcgtggcgt ttcacaattt gggcttgacc attgtggacg aacagcaccg tttcggcgtt
     7021 gcccaacgcc tcgccctcaa aaacaaaggg cgcgaagtcc atcagctgat gatgtccgcc
     7081 acgcccatcc cgcgcacgct tgcgatgagt tttttcgccg atttggacgt gtccgtcatc
     7141 gacgaattgc cgcccgggcg cacaccgatt aaaacgcgcc tcgtcaacaa cgtccgccgc
     7201 gccgaagtcg aaggcttcgt cctcggcact tgccgaaaag ggcggcaggc gtattgggtc
     7261 tgcccattga ttgaagaaag cgaaaccctg caactgcaaa ccgccgccga aaccctcgcc
     7321 cggcttcaga cggcattgcc cgaactcaat atcggactgg tacacgggcg catgaaggcc
     7381 gccgaaaagg ccgaagtgat ggcgcggttt tcttcggggg ggctgaacgt cttggtcgcc
     7441 accaccgtca tcgaagtcgg cgtagatgtg cccaatgccg ccctgatggt catcgaacac
     7501 gccgagcgca tgggcttggc gcagcttcac caattacgcg gacgggtagg gcgcggcgcg
     7561 gcggaaagcg tgtgcgtcct cctgtttgcc gaacccttgg gcgaactcgc caaagcgcgg
     7621 ctgaaagtca tctacgaaca caccgacggc ttcgaaatcg cccgccaaga cctcaatatc
     7681 cgcggcccgg gcgagtttct cggcgcgcgc caaagcggcg tgcctatgct gcgtttcgcc
     7741 aaactcgaag aagacctgca ccttttggaa caggcgcgcg aaaccgcccc gatgctgatt
     7801 gagcaaaacc ccgaaatcgt cgaagcgcat ttggcaaggt ggctttccgg cagggaaggg
     7861 tatttgggcg tgtgagcaaa atgccgtctg aaatattaga aaaaaaagtg taacggaaat
     7921 ctg
//
LOCUS       DAXHTR010000101         7897 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.101, whole genome shotgun sequence.
ACCESSION   DAXHTR010000101 DAXHTR010000000
VERSION     DAXHTR010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7897
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.101"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..108
                     /locus_tag="ACFNML_001516"
     CDS             <1..108
                     /locus_tag="ACFNML_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HFC6392871.1"
                     /translation="VPKKAETISNKLFFTHHPKRQTEFAVRQIRQARAE"
     gene            234..1529
                     /gene="hisS"
                     /locus_tag="ACFNML_001517"
     CDS             234..1529
                     /gene="hisS"
                     /locus_tag="ACFNML_001517"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFC6392872.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVNRWTRAYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLLLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRAQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAEDLTHTLQQWKNA"
     gene            1530..2159
                     /locus_tag="ACFNML_001518"
     CDS             1530..2159
                     /locus_tag="ACFNML_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HFC6392873.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RAASQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISAAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFS
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            2313..3770
                     /gene="der"
                     /locus_tag="ACFNML_001519"
     CDS             2313..3770
                     /gene="der"
                     /locus_tag="ACFNML_001519"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFC6392874.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEKEEEEAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            3801..4700
                     /locus_tag="ACFNML_001520"
     CDS             3801..4700
                     /locus_tag="ACFNML_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFC6392875.1"
                     /translation="MWFKQISFYPLNKEKLPEADVLADKLAEAEFTHCQGLEWFSEGF
                     AAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAASLAFASQ
                     ILMAESVSTMLEELVSYLGGWQD"
     gene            4752..5168
                     /locus_tag="ACFNML_001521"
     CDS             4752..5168
                     /locus_tag="ACFNML_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MORN repeat-containing protein"
                     /protein_id="HFC6392876.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYDGGVGKDRLPS
                     GKGIWRCRDGRGYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            5234..5800
                     /gene="dcd"
                     /locus_tag="ACFNML_001522"
     CDS             5234..5800
                     /gene="dcd"
                     /locus_tag="ACFNML_001522"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFC6392877.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICETSYKDRNGKYMGQTGVTLPKA"
     gene            complement(5864..6568)
                     /locus_tag="ACFNML_001523"
     CDS             complement(5864..6568)
                     /locus_tag="ACFNML_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HFC6392878.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEAVQDTMSA
                     RLQVAAEGRDKNAVNAEFVKKFNKFIRKSKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDELNRFIADVQADAALEYTDFHVSRERRNEVIDQVSKDAILRFK
                     ARADKLSGILGTSGYKIVKLNLGHIGSHIAGEGAAQAKMLRAMPMAASYGVEGTDSAA
                     PGVEEISISVNGTVQF"
     gene            6714..7897
                     /locus_tag="ACFNML_001524"
                     /pseudo
     CDS             6714..7897
                     /locus_tag="ACFNML_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678130.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         2099 a         2175 c         1897 g         1726 t
ORIGIN      
        1 gtgccgaaaa aagccgagac aatttcaaat aaattgtttt tcacacatca tcccaagcgg
       61 cagacagagt ttgcagtccg acaaatcagg caagcacgtg cagagtaagt caaatgccgt
      121 ctgaaaactg tttcagacgg cattttaccc gtccgcaaac cacccccaac ctcacaaacc
      181 cttaaaaatc cgctataatc gcccgcaatt tgatttaagc attcttactt gaaatggcac
      241 aaaaaatcca atccgtcaaa ggcatgaacg accttctgcc tgtcgagcaa aaagatttca
      301 aactgacggc tgcgttttgg caggcgtttg aggatacggt aaaccgctgg acgcgcgctt
      361 acggctatca gcaaatccgt acgccgattg tcgagcaaac cggtttgttt gtccgctcca
      421 tcggcgagga aaccgatgtg gtcggcaagg aaatgtacac cttttccgat tcaaacgatt
      481 ctttgagttt gagcctgcgt ccggaaggta cggcttcttg tctgcgtgcg gtggtcgaac
      541 acaaccttct gtacaacagc ccgcaaaagc tgtggtatat ggggccgatg ttccgccgcg
      601 agcgtccgca aaaaggccgt tatcgtcaat tccatcaggt cggtatcgag gctttgggtt
      661 ttgaagggcc ggatatcgat gcggaaatca tcgcgatgtc tgccgacttg tgggaaaaat
      721 tgggtatccg cgaatacctg actttggaaa tcaacagctt aggcaaccgt gaggagcgag
      781 cggcacaccg tgcggcgttg gttgaatatt tgacacgcta tgaagataaa ttggacgaag
      841 acagcaaacg ccgtctgaaa accaatcctt tgcgtgtttt agatacgaaa aaccctgatt
      901 tgcaggaaat ctgcaacgcc gcgccgcgtt tggtggatta tttgggcgag gcttcgcaaa
      961 accactatgc acgcttcaag gcgatgttgg acggtttggg tatccaatat attgaaaatc
     1021 cgcgcttggt tcgcggtttg gattattaca atcagacggt ttttgagtgg acgaccgaca
     1081 aactcggcgc gcaggcaact gtgtgcggcg gcggccgtta cgatggtttg attgaggaac
     1141 ttggcggcaa acctgcgccg tccatcggct ttgcgatggg catcgaacgg ctgctgcttt
     1201 tggtgagcga atacggctcg ctggaagtga acgctgcgcc tgatgtctat gcaatgcacc
     1261 aaggcgaaag ggcggacttg caggtgatga aatacgcgca agccttgcgc gcgcaaggtt
     1321 tcaatgtaat gcagcattcc ggctatcaaa gcctgaaagc gcaaatgaaa aaagccgaca
     1381 acagcggcgc gcgctttgcc ctgattgtcg cgcaagacga actggcgaac ggtacggtta
     1441 cgctcaaaga catgaacggc gcacacggtc agcaaactgt cgccgccgag gatttaaccc
     1501 atactttaca acaatggaag aacgcataaa tggcagccca tctcgaagaa caacaagagt
     1561 tagacaactt taaatatttt tggaaaacca cgggcaaatg gctgtttgcc ctgctgattt
     1621 tggcagcact cggctacttg ggatacacgg tttaccaaaa ccgtgcggct tcccaaaatc
     1681 aggaagcggc ggcggtgctg gcaaacatcg tggaaaaggc gcaaaacaaa gccccgcaaa
     1741 gcgaaattaa tgccgaattg accaaactcc aacaaagcta cccgcattct atttccgccg
     1801 cccaagccac gctgatggcg gcggcaaccg aatttgacgc gcagcgttac gatgttgccg
     1861 aaggccattt gaaatgggtg ttgtccaacc aaaaagacag cctgattcaa gcgttggcgg
     1921 cgcagcgtct gggcgttgtg ttgttgcaac aaaaaaaata cgatgccgcg cttgccgcgc
     1981 tcgacacgcc ggttgaagcg gacttctccc ccctgctgat ggaaaccaaa ggcgatgtct
     2041 atgccgcaca gggaaaaagc caggaagcct taaaaaacta cggtcaggct ttagaaaaaa
     2101 tgcctcaaga ttctgtcggt cgtgaattgg ttcaaatgaa actcgattcg ctgaaataaa
     2161 tgccgtctga aatgccgtcc attatttatc agacggcatt ttaccctccc tctttatggc
     2221 ctcttgccgt gaagaaccta tttaccgtgt ccaacggaca cctgctcgtg ttcagttctc
     2281 ccgaacggca cttgacacag aaagactcaa tcatgaaacc aaccatcgcg cttgtcggcc
     2341 gccccaacgt cggcaaatct actttattca accgtttgac gcgcaccaaa gacgcactcg
     2401 tacacgacct gcccggcctg acccgcgacc gccattacgg acacggcaaa gtcggcagca
     2461 aaccttattt ggtcatcgat accggcggtt tcgagccggt tgtggacagc ggcattttgc
     2521 acgaaatggc aaaacaaacc ttacaggctg tcgatgaagc cgatgcggtt gtgtttttgg
     2581 tggacggccg taccggtctg acgccgcaag ataaaatcat tgccgaccgt ttgcgccaaa
     2641 gcccgcgccc cgtttatttg gccgtgaata aaggagaagg cggcaataga gccgtgcttg
     2701 ccgccgagtt ctacgaactt gctttgggcg acccttatgt tatttcaggt gcgcacggtg
     2761 acggcgtgta ttatctgatt gaagatattt tggaaacctt ccctgagcct gaaaaagaag
     2821 aggaagaggc gaaacatcct gtttttgccg ttatcggtcg tccaaatgtc ggtaaatcaa
     2881 ccttggtaaa cgccattctc ggcgaagagc gcgtcatcgc cttcgatatg gcaggtacga
     2941 cgcgcgacag tatccatatc gatttcgagc gcgaaggcaa accgtttacc atcatcgata
     3001 ccgcaggtgt gcgccgtcgc ggcaaagtgg atgaagcagt ggaaaaattc tccgttatca
     3061 aagcgatgca ggcggttgaa gcggcaaacg tcgctgtttt ggtattggac gcgcagcagg
     3121 acatcgccga ccaagatgcg acgattgcag gtttcgcttt ggaggcaggc cgtgctttgg
     3181 tggtggcggt caataaatgg gacggcatca gcgaagaacg tcgcgagcaa gtgaaacgcg
     3241 atatcaaccg caaactgtat ttcctcgatt ttgccaagtt ccactttatt tccgcattga
     3301 aagagcgcgg tatagacggt ttgtttgaca gcattcaggc tgcctacaac gcggcgatga
     3361 ttaagatgcc gacgccgaaa atcacgcgcg tattacaaag cgcgatcgag cgtcagcaac
     3421 cgccgcgtgc cggcttggtg cgtccgaaaa tgcgttatgc ccaccaaggc ggcatgaacc
     3481 ctcccgtaat tgtggtacac ggcaattcgc tgcacgcgat ttccgacagc tatacgcgct
     3541 atctgaccca aacgttccgc aaagccttca atctgcaagg cacgccgctc agaattcaat
     3601 acaatgtttc ggaaaacccg tatgaaaatg cggaagacaa accgaagaaa aaaccgctgc
     3661 gccgcgtcag cctgagcaac cgtattgaga aacgcgaagg ccgcaaagaa gagaaaaacc
     3721 gcttcaagaa gaaaaccaaa gtcagtgtga aaaaacaatt cagcaaataa ttcccgacat
     3781 ttcaaacaat aggaaacatt atgtggttca agcagattag tttttatccg ctcaacaaag
     3841 aaaagctgcc tgaggcagac gtacttgccg acaaacttgc tgaagctgaa tttacccatt
     3901 gccaaggctt agagtggttc agcgaaggct ttgccgcacc ggtttcgttc tctcccgaac
     3961 tcgttttccc tgccgacttt accttgcgcg tcgccctgaa aaaagaagaa aaagtcctgc
     4021 ctgccggcgt catccgcgat attttggaag agaaggtggc ggaaatccaa aacaatgaag
     4081 cccgcaatgt cggtcgcaaa gaaaaacaag agcttaaaga gcaaattaca gacgacctgc
     4141 tgccccgagc gtttacccgc agcagccgta cagaagcggt gtttaacacc cgccacggct
     4201 acctgctcgt caataacgcg gcttccgcca aagcagaaaa catcctgacc aagctgcgcg
     4261 aagctttggg cggtttggaa gcctcgctgc cgaataccaa acaatcgccc tcttctctga
     4321 tgaccggctg gctgttgcaa gggcattgcg aaggcggttt tgaattggac agcgattgcg
     4381 aactcaaagg tacgggcgat attgttcccg tcgtcaaagt atccaaacaa gatttaaccg
     4441 ccgacgaagt ggttcaacac gtcaaaaacg gtaaaaccgt aacccagctc ggtttggttt
     4501 ggcgcgaaca aatcgccttt atcctcactc aagacttcac actcaagcgc atccaatacc
     4561 tcgacgtatt gcaggaagaa gccgaaagca acggcgacga tgccgccagc cttgccttcg
     4621 cctcgcaaat cctgatggct gaatccgtca gcaccatgtt ggaagagctg gtttcctatt
     4681 tgggcggctg gcaagattga tttttcagac ggcttttata ccgtcagcca aaatccaatg
     4741 gaataaccct tatgcttaaa catctcgcat tcctactgcc cgccatgatg ttcgccctcc
     4801 ccgccgcgtc cgccgttctg acttcctatc aagaacccgg ctgcacctac gacggcggag
     4861 tcggaaaaga caggcttcct tcaggcaaag gcatatggcg ttgccgggac gggcgcggtt
     4921 ataccggttc gtttaaaaat ggcaaattcg acggacaagg cgtttatacc gttgccgcca
     4981 accgcgaaat atttatcgaa ccgttcaatt ccgacagtac caaattccgc aacatggtac
     5041 tctcgggcac gttcaaaaaa ggcttggcac acggcagatt taccgtctcg caaaacggcg
     5101 aaaccctctt cattatgaaa tgcgaaaacg gcatgattaa agaagtgaag ctgcccaaaa
     5161 acaaataagc cccgttttca gacggcatcc cccaatgctt tgtccgaaca ctgcaagaaa
     5221 caaggaaaac attatgagca tcaagtccga caaatggata cgccgcatga gcgaagaatt
     5281 cggcatgatc gaccctttcg agccgaacca aatcaaagaa gccgacggca aacgcatcat
     5341 ctcctacggt acgtccagct acggctacga catccgctgc gccaacgaat ttaaaatctt
     5401 caccaacatc aacagcacca tcgtcgatcc caaaaacttc gacccaaaaa acttcgttac
     5461 cgttgaagac gactgctgca tcatcccgcc taattccttc gcactggcgc gcacggtcga
     5521 atatttccgc atcccgcgca acgtcctgac cgtctgcttg ggcaaatcca cctacgcccg
     5581 ctgcggcatc atcgtcaacg ttaccccgtt cgaaccggaa tgggaaggct acgttaccct
     5641 cgaattttcc aacaccaccc ccctgcccgc caaaatctac gccggcgaag gcgtggcgca
     5701 agtcctcttc ttcgagagcg acgaaatctg cgaaacctca tacaaagacc gcaacggcaa
     5761 atacatggga caaaccggcg taaccctgcc caaagcctga aatataaatg ccgtctgaac
     5821 ccttatcgtc gggtttcaga cggcatttgc cgttcacctc aaatcagaac tgaaccgtcc
     5881 cattgacgct gatgctgatt tcctccacac ccggcgcggc ggaatccgta ccctccacgc
     5941 cgtaacttgc cgccatcggc atggcacgaa gcatttttgc ctgagcagct ccttcacccg
     6001 cgatatggct gccgatgtgt cccaaattta atttgacgat tttataaccg gacgtaccca
     6061 atatgcccga caacttgtct gcacgcgcct tgaaacgcaa aatagcatct ttactgacct
     6121 gatcgatgac ctcgtttcgg cgttcgcgcg acacatggaa atccgtatat tccaatgcgg
     6181 catctgcctg aacatcggca ataaaacggt ttaactcatc aaaatccctc ccttcgacct
     6241 taaattccgc acgctcctcc caacctgttt gaatgcgtct gccgttggta tattgatagc
     6301 gcggcatcgc actgcgcgat accaattcgg ttttaaagct accatttttc gattttctga
     6361 tgaacttgtt gaatttttta acaaactcgg cattgacggc atttttgtcc cgtccttccg
     6421 ccgccacctg taaacgcgcg gacattgtat cctgaaccgc ctcgacaccg gccgattcgg
     6481 aaaattcgac aatattgtaa ttcaatgctt cagccgctgc cggaaaagat accgccaata
     6541 gggaagccgc caaaatagaa cgcagcatat ccgtatcctt acttataaaa ccgccaatct
     6601 agcatatcgg cgcgctaaat tgcgttagcg taaggcaaca atttaaatat tcggtctgcg
     6661 tctgtcttat aataaccgcc tttccgatac ggaatcccac tatgcccaaa cccttgcacg
     6721 tcctcgtcat cccctcatgg tatccgcaat ccgaacagga tgtggacggg atttttttca
     6781 aaatcaggca ctggcattgc agaggaaagg catcaaaacc gccgtgcttg caccgatgtt
     6841 ccgctacttg cggaaagaaa cagcaagcat cctgaccgac cttacggttt tgcaaaatat
     6901 cggcaaagcg gtttggacat ctatgcatgg cacggcatgt atttcttccc ccgctttccg
     6961 ttcatcgaca tcgaccgcat ccgctgggtg cgcgtgggtt tgaaagcctt caaacactac
     7021 atccgcgaaa acgggctgcc cgacctgatt cacgcccacc gcatgaacta tgccggcata
     7081 ctcgcccaaa aaatttccca aaaatacggc atcccctatg tcgtcacgaa acacagcagc
     7141 accattacgc gcggtctgat ccgtccccac caatggcagc ctatgaaaaa tgcggcggcg
     7201 cacgccgccg cacgtctcgc catcagccgc catttcgcac acgtcctgca acacaaatac
     7261 ggctgcgaat ggcagtacct ccccaacata ctgggcggaa tattcaaccg accgtttgaa
     7321 caaagagaaa aaaacaacaa accgcatttc gtgttctgca ccgtctcgca ccttcgccgt
     7381 ctcaaaggac acgatgtcct gctcactgcc tttgcccggg cgttggcaca atgcccgcaa
     7441 ctgcgtctaa acatcggcgg cagcggacag gaagaacagc ggctgaaaca gcaggcggca
     7501 gacttgggca ttacccatgc cgttacattt ttgggcgcac tgcaacccga agcagtcttg
     7561 gatttgatga agaacagcga cgcatttgtc cttgccagcc gcaccgaaac cttcggcgtg
     7621 gtctatatcg aagcactgtc ccaaggattg cccgtcattg caacacgctg cggcggtgcg
     7681 gaatctattg tttcagacgg caacggatat ttggttcctg ttgacgacga cgatgccctt
     7741 gccgacgcac tcatcaaaat gtatgaacac cactctgatt tcgaacctgc ccgacttagg
     7801 gaaaactgtc tgaacgaatt tggcgaaaat gccgttatag gcaggttgat cggcattttc
     7861 cgacaggcaa tcgcagaata cggtaagaaa ataccgg
//
LOCUS       DAXHTR010000102         7890 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.102, whole genome shotgun sequence.
ACCESSION   DAXHTR010000102 DAXHTR010000000
VERSION     DAXHTR010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.102"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(94..774)
                     /gene="tsaA"
                     /locus_tag="ACFNML_001525"
     CDS             complement(94..774)
                     /gene="tsaA"
                     /locus_tag="ACFNML_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFC6392879.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLVSAAEVCIELNPKFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDGTPIVDIKPYIPFVESKPDAASGFVSGKPVELEVV
                     WQENIGAENLSANTKNLISQSIAQDPRPAYQNIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            888..1523
                     /locus_tag="ACFNML_001526"
     CDS             888..1523
                     /locus_tag="ACFNML_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HFC6392880.1"
                     /translation="MIVSIDVDAQKTFTPLCPDELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHMVAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSTKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIIGGLATDYCVKTTVLQL
                     LKGGRWQVVVNEAACRGIAPETIETAWQEMHSAGAIILKNADEINKYINNQ"
     gene            1560..2528
                     /gene="waaC"
                     /locus_tag="ACFNML_001527"
     CDS             1560..2528
                     /gene="waaC"
                     /locus_tag="ACFNML_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HFC6392881.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPDVELHWLCEAGFADI
                     ARLHPFVKKIHVMKWRQWRKHLFRAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVETYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTAPEAGRLKNLEQPYYAALHATSRDSKLWPVENWRALLQKLNEEQQCNIYLPWGS
                     EVEKTRAEQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQTLMDCVAADKG"
     gene            2754..3503
                     /locus_tag="ACFNML_001528"
     CDS             2754..3503
                     /locus_tag="ACFNML_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFC6392882.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDTKLESLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVHIEGGEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKTLEKLTPDDLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            3514..4449
                     /locus_tag="ACFNML_001529"
     CDS             3514..4449
                     /locus_tag="ACFNML_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFC6392883.1"
                     /translation="MSVLIIVEHDNKQLNPTTLHAVTAAAKLGKIDLLVAGSGASAVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYCYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQADSEKLVLTFRATVFDAVAAQGGN
                     AEVINVEATPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            4512..5123
                     /locus_tag="ACFNML_001530"
     CDS             4512..5123
                     /locus_tag="ACFNML_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HFC6392884.1"
                     /translation="MITTYKTITSSTQAEFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGMQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVPGLIHAYKEATAQALAVAEVVEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     VLERDLQLDCRLTVSLSLVNYEACVAAWKSTRQIEVNTEKPFE"
     gene            complement(5175..5492)
                     /locus_tag="ACFNML_001531"
     CDS             complement(5175..5492)
                     /locus_tag="ACFNML_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HFC6392885.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKHAVALIRDMDNFYT
                     HEYQACHRAIEDGAEVDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            5650..6921
                     /gene="purD"
                     /locus_tag="ACFNML_001532"
     CDS             5650..6921
                     /gene="purD"
                     /locus_tag="ACFNML_001532"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFC6392886.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVASGNAGTAIEPKLQ
                     NIDLTAHQDLIEFCRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARIVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPHTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGKLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            6955..7557
                     /locus_tag="ACFNML_001533"
     CDS             6955..7557
                     /locus_tag="ACFNML_001533"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFC6392887.1"
                     /translation="MTNVLQTVFYIMLFPRIIAASAQRKLSVYLKKGGLVAYPTESCY
                     GLGCLPTLAKALGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPA
                     PKTFLLSAKSCVLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRAC
                     RTEREVRRQFGRDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(7628..>7890)
                     /locus_tag="ACFNML_001534"
     CDS             complement(7628..>7890)
                     /locus_tag="ACFNML_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6392888.1"
                     /translation="DSLSSCDKLDVSGFIYYRINHSKEFADRQNHINGIENFWNQAKR
                     VLRKYNGIDRKSFPLFLKECEFRFNFGTPSRQLKILQDWCGI"
BASE COUNT         2089 a         1559 c         2112 g         2130 t
ORIGIN      
        1 ttaatccgct ataacttgat attgcggctt atggcgaaca aacaacagcc gtttgttcaa
       61 ctgctatgcg gcaaaaccgg atttttgccc gatttaaagc ggggttgggg aaagatcaat
      121 aacggttgca cggttttcct cgatttgaaa tctgacttcg taatctgcaa tattcatcac
      181 ataaatccgt tcgggaatat tctgataggc ggggcgcgga tcttgggcaa tgctttggct
      241 gataaggttt ttggtgtttg cagataaatt ttccgcgccg atgttttcct gccaaacgac
      301 ttccaactct acgggtttgc cgctgacgaa accggatgcg gcatcgggtt tggattcgac
      361 aaaggggata taaggtttga tgtccacaat cggtgtgccg tccagcaggt ctgcgccgct
      421 gcaatagagg cggacgggtt tgccggtttc gatgcgttcg agtttcagga gcgagagtcc
      481 gagatggttg gggcggtggg ggctgcgcgt ggcgaacacg cccatttttt gtttgccgcc
      541 gagccgtggc gggcgcacca tttgcgccca gccttcatcc aatacgccgt gaaaaataaa
      601 acttatccac acataatcga aatcttccag cccgcgcacg ctgtctgcgg tgaatttggg
      661 attcagctcg atgcatactt ctgcggcaga gaccaaaccg ggctggcggg cgatgccgaa
      721 tttctgtttg tagggcgagc gggcggtgcc gatgggggta atggtataag tcataaggtt
      781 gtggataagt tttttggaaa aaccttatct tatcacaatc ggttttaccg cttttgggca
      841 gtaatttgtt ataatacagg gcattttaat cgcgttgaaa taatgagatg attgtttcca
      901 ttgatgttga tgcacaaaaa acgtttacgc cactgtgtcc tgacgaactg cccgtgaacg
      961 aggggcattt gattgtcgag gagttgaatg cgcaagccgc tttggcggat ttgcgcgtga
     1021 tgacgaaaga tgcgcatcat atggtagcga aatggcttgt ggataaccct gttgatatgt
     1081 tgaagccgac aggtttgcct gatgcggatt tgacttgggt ggctcatgcg atggtcggta
     1141 cgcgcggcta tgaattattg gacggactgc cttctaccaa agaatacgat tattgcgttt
     1201 ggaaaggtgt tgatcctgaa ttgcatcctt acggcgcgtg ttttcacgat attgaggaaa
     1261 aactaagcac agggctgatt gaatggctgc gttgtcaaaa tacggatacg gttattatcg
     1321 gtgggttagc tacggattat tgtgttaaaa caacggtttt gcagttactc aaaggcggtc
     1381 gttggcaggt tgtcgtcaat gaagcggctt gtcggggtat tgcgccggaa accatcgaaa
     1441 cagcatggca ggaaatgcat tctgctgggg caatcatctt aaaaaacgct gatgaaataa
     1501 ataaatatat taataatcaa taagttaaaa ttgtttcacg tgaaaccccc ttcctaaata
     1561 tgaaaatttt gcttgtccgc ttgtctagta tgggcgattt aattcacact ttgcccgcaa
     1621 tcgaagattt ggcgcgacaa tgtcctgatg tagaactgca ttggctatgt gaggctggat
     1681 ttgcagatat tgcgcgcctg catccgtttg taaaaaaaat ccatgtgatg aaatggcggc
     1741 aatggcgcaa acatctcttt cgggctgaaa cttggcggga aatgggtcat ctgaaacaga
     1801 ctttgcggca ggaagtattt gatttcgtat tggacagtca aggtctgatt aaaagcgcgt
     1861 gtttcgccaa aatggcaaaa tccccgattt gtggtttgga taaaaacagt gcgcgcgagg
     1921 gatgggctgc tttggcgtat gtagaaacat acgctgtacc gaagggaaaa aatgccgttt
     1981 ggcgcaaccg tgaactgttt gcccaagtat ttgggtatgt aatgcctgaa acgcaggtat
     2041 ttggcttgac tgctcctgag gcaggtcgtc tgaaaaattt agagcagccg tattatgcgg
     2101 ctttgcacgc gactagccga gacagtaaat tatggcctgt ggaaaactgg cgggcgttac
     2161 tgcaaaagtt gaatgaagaa cagcaatgca atatttacct gccttgggga agtgaagttg
     2221 aaaaaacgcg tgccgaacag attgcagacg gactgccgtt tgctattgtg tgcgacaaaa
     2281 taaatttatt gcaggcagcg tatctgctga aatacgcggt cggaattgtc ggcgtggata
     2341 ccggtttgct gcatttggca aatgccttgg aaaaacctgt ggtcggcatt tataccgata
     2401 ccgatccgat taaaacaggc gttcaggttt cgccaattgc aaaaaatttg ggcaatatcg
     2461 ggcagattcc gaccgcagat ttggtttatc aaacgttgat ggattgtgtt gcagcagaca
     2521 aaggctaaag ggttgtctga aactgatata gcacctgata tttttcagtt tgttaagcat
     2581 aagaatgtat atctaccagc ctgagtaaat gctttaaaaa tggtataatc ggcaagcatt
     2641 atccgaaaga tttttgaatg aattcgtttt taaggaaaac ggatttggtt ttatagggaa
     2701 gaaggtttgg tttcgctgga tggtgaaagc agcgttcttt ttaggagggt tcaatgaaag
     2761 cactggtcgc agtaaagcgc gtagtggact acaacgtcaa agttcgtgta aaagccgatg
     2821 gttcggatgt ggatatcggc aatgtcaaaa tgtcgatgaa tccgtttgac gaaatcgctg
     2881 tggaagaagc tgtccgtttg aaagaagccg gaaaagtaag cgaaatcgta gcggtttctt
     2941 tgggtgagaa aaaatgcgaa gaaaccttgc gtacagcttt ggcgatgggt gccgaccgtg
     3001 ccattcatgt tgaaaccgat actaaactgg agtctctggc cgttgctaag ttgctgaaag
     3061 ccgttgcgga caaagaaaat ccgcaaattt tctttttggg caaacaagcg attgatgatg
     3121 atgccaatca agtggcgcaa atgctggcag ctttgctgaa tgcggcgcaa ggtacgtttg
     3181 cttcaaaagt acacattgaa ggtggtgaag tacagattgt gcgcgaaatt gatggcggcg
     3241 aagaaaccat agcattaaaa ctgcctgctg ttatcagtgc tgatttgcgt ttgaacgagc
     3301 cgcgctttgt caaactcccc aatattatgg cggcaaagaa aaaaactttg gaaaaactga
     3361 ctcctgacga tttggttgcc gacatttcac ctcgtttgaa aacggtgaaa tttgccgaac
     3421 ctaaggcgcg tcaggcaggc gtaaaagtag caagcgttgc cgaattggtt gaaaaattga
     3481 aaaacgaagc caaagtgatt tgaggaggct gaaatgagcg tattgattat tgtcgaacac
     3541 gacaacaaac agttgaatcc taccactttg catgctgtta ccgctgccgc taagctgggt
     3601 aaaatcgatt tattggttgc cggaagcggt gcatctgctg tagtagagtt cgcaaaacaa
     3661 gtggcgggtg tggaaaaagt tttggttgca gatgctgccc attatgccga aggtttggct
     3721 gaagagttgg ctccgctggt tgtcaaattg gcagcggatt actgctatgt tgcagcaacg
     3781 gcaactacat tcggtaaaaa ccttttgcct cgcgtagcag ccttattaga cgtaccgcaa
     3841 atttctgatt tgaccgaaat tgtggataac acgacttttg tgcgccccat ttatgcgggt
     3901 aatgcatttg aaaccgtgca agctgattca gaaaaattgg tgctgacctt ccgtgcgacg
     3961 gtttttgacg cagtggcggc gcaaggtggt aatgctgagg taattaatgt tgaagcaacc
     4021 cctgcccaaa acctgagccg ttttgttaac cgtcagcttt cccattccga tcgtcctgaa
     4081 ctgactcagg caaaagtgat tgtttccggt ggtcgtgcgt tgggtagtgc ggaaaaattc
     4141 aatgaagtgc tgacaccgtt ggcggatgtt ttaggtgcgg caatcggtgc atcccgtgcg
     4201 gcggttgatg ccgaatatgc gccaaacgat gctcaagtcg gacaaaccgg taaagtggtt
     4261 gcgccgcaac tctattttgc aatcggtatt tcaggtgcga ttcaacacgt tgccggtatg
     4321 caagacagta aggtgattgt tgcaatcaat aaagatgctg atgcgccgat tttcaatgta
     4381 gccgattacg gattggttgg cgatttattt gaaatcgttc cacaattaac agaagcattg
     4441 aaaaattaat gtttcacgtg aaacgttcag acgaccttgt tattaatgag gtcgtctgaa
     4501 aacatttcag catgattacg acttataaaa ccattacttc ctcgacgcag gctgagttta
     4561 aagataaagg cagccgtttt attgcatttg cctatccgat tcggacattg gctgatgtga
     4621 aaaaatacct cgatccgtta aaggaagagc atcataaagc acgacactgg tgctatgcct
     4681 atcgtttggg tgtggatggc atgcaatttc gtgccaacga tgatggagag ccatcaggaa
     4741 gtgctggacg accaattttg ggacagattg attcggtggg tattaccgac gttttggtcg
     4801 tggtcgtccg ctatttcggt ggtactttgt taggtgttcc aggtctgata catgcgtaca
     4861 aagaggcgac ggctcaagcg ttggcagttg cagaagtagt tgaaaagaat attgaaaaaa
     4921 ctgtttggct gaaatgtgaa tatccgtttt tgaatgaagc gatacgcatt gctaaacaat
     4981 atcaggcgga tgtattggag cgggatttgc agttggattg ccgattgacg gtcagtttgt
     5041 cgttggtaaa ttatgaggcc tgtgttgcgg cttggaagag tacacggcag attgaagtaa
     5101 atacggaaaa gccttttgaa tgaaaggtcg tctgaaatgc tttgtgatgg tatttcagac
     5161 gacctcttcg ctgactaatc ttcttgtgta tatttgtcta aggcatcgac gctggagcgc
     5221 aaatataacc aagccagttg atggaattcg cccagcgcgt tccatagtgc atcttcactc
     5281 ataatgctgt attcgccttc tgtgcgcaaa tccacctccg ctccatcttc aatagcccga
     5341 tgacaagcct gatattcgtg ggtataaaaa ttgtccatat cgcgaatcaa agcgacggca
     5401 tgtttccaac ggcggatgtc ttcttccaat tgcggtagta aatgacacca ttcgcggtat
     5461 ttgctttgga tttcatcaat acgtttttgc atggttgctt gtcctttttt aatttatgaa
     5521 taagttgaat aggttgcaga gtggaatgaa gcggtctttt gtttgattct actaaaattt
     5581 cttcagatgg gataaaagtc cctttgtatc gagttttcgt taaaagtaca tatttgtcaa
     5641 ggagatggca tgaaactgct ggttatcggt aatggcggtc gcgaacacgc gctggcttgg
     5701 aaattggcgc agtcgcctaa agtggaaaca gtatttgttg cgtctggtaa tgccggtaca
     5761 gcgattgaac ccaagctgca aaacatcgac ttgactgcgc atcaggattt gattgaattc
     5821 tgtcgtaaag aaaatattgt ttttaccgtc gtcggccctg aagcgccttt ggcggcaggt
     5881 gttgtggatg atttccgtgc tgcaggacta aaaatattcg gtccgacaca atatgcggcg
     5941 cagttggaaa gttccaaaga tttcgccaaa gcatttatgg cgaaatacaa tattccgacc
     6001 gcgcaatatc aaacctttga aaacgccgat gccgcacatg attacgtcaa tcagaaaggt
     6061 gcgcctatcg ttatcaaagc cgatggtttg gcggcaggta aaggcgtgat tgtggcgatg
     6121 actttggatg aagcgcatgc tgcgattgat gatatgctgc tggacaacaa aatgggcaat
     6181 gccggcgcgc gtattgtgat tgaagatttc ctgcaaggcg aagaagcgag ttttatcgtc
     6241 atggttgatg gcaataatgt actgcctatg gcaaccagcc aagaccacaa gcgtctttta
     6301 gacggcgaca aaggtctgaa tacgggcggt atgggtgcgt acagccctgc gcctgtggta
     6361 acgcctgttg tgtacgaacg cgcgatgaat gaaattattt tgccgaccgt agcagggatg
     6421 aaagcggagg ggcatgagtt caccggtttc ctgtacgcag gtttgatgat tgatcaaagc
     6481 ggtgcacccc atacgattga gtttaactgc cgtttcggcg atcctgaaac ccagccgatt
     6541 atgagccgac tgaacagcga cttatcggat ttggttgaag cggcaataga cggcaaactc
     6601 gatagcgtaa ccgcggaatg gagcccgcaa actgccgtgg gcgtggtact ggcggcgcaa
     6661 aattaccccg aaacgcccaa aaaaggcgat gttatttccg gtctggatgc tgccaaccaa
     6721 gtaggcaaag ttttccatgc aggtacaacg gcaaacgaga aaggtgacgt attgaccaac
     6781 ggcggacgcg tattgtgtgt tgtgggattg ggcgataatg tggcgcaagc gaaagctaag
     6841 gcatatggcg cgttggaaaa aatcagtttc gacgggatgc aataccgtaa agacattgcc
     6901 gataaagcga ttaaccgtta attagaaaga ggatgatgcc gtctgaagcc aagtatgact
     6961 aatgtgcttc agacggtatt ttatattatg ttatttccga gaattatcgc agcatcagcg
     7021 caacgtaagt taagcgttta tttgaaaaaa ggtggtttgg tcgcttatcc gaccgaatct
     7081 tgttatggct tggggtgtct tccgacatta gccaaagcac tcggtaagtt ggcgcatttg
     7141 aaaaaaagac cgcaacacaa gggtatgatt gtcatcggga atcaatttga acaattgcag
     7201 cctttacttc aaatgccgtc tgaaaatctt caggatatgc ttagaaaaga atggcccgcc
     7261 cctaaaacgt tcctattatc cgccaaatcc tgtgtcttac ccgagttacg cgggaaacag
     7321 cgcagcaagt tggcggtcag ggttcccgcc catgtaggtg cgcgccgctt gtgtcaggct
     7381 ttgcaaacgc ctttggtctc gacttcgtgc aaccgcgcag gcaaacgcgc gtgtcggacg
     7441 gagagggaag tcagaaggca attcggacgc gatgtgtgga ttgttggtgg gagaatcggc
     7501 aggcagaaat cgccgagtca gattattgac ggggaaacag gtaaacgttt gcgttagata
     7561 tgggtacttg aatgaaaact tttttgcata cacaaagaaa aaattttggg gctgtactag
     7621 attagcccta aatcccacac caatcctgta ggatttttag ctgccgggac ggtgtgccga
     7681 agttaaatcg aaattcgcat tctttcaaga acagcgggaa agatttgcga tcgattccat
     7741 tgtattttcg caagacacgt tttgcctgat tccaaaaatt ctcaatgccg ttaatgtggt
     7801 tctgacggtc tgcaaattcc ttggaatggt tgatgcggta ataaatgaaa ccgctcacgt
     7861 ccaacttgtc gcagctgctc agactatcgg
//
LOCUS       DAXHTR010000103         7792 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.103, whole genome shotgun sequence.
ACCESSION   DAXHTR010000103 DAXHTR010000000
VERSION     DAXHTR010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.103"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            533..2041
                     /gene="nadB"
                     /locus_tag="ACFNML_001535"
     CDS             533..2041
                     /gene="nadB"
                     /locus_tag="ACFNML_001535"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFC6392889.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEAGAGLCDEAAVRTILSQGKLAIEWLLAQGVAFDQ
                     NNNGLHLTREGGHTCRRIAHVADYTGEAVMQSLTVQIRCRPNIRVCEQQMALDIQTES
                     GAACGLTVLDCRTQATYSIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPYYDRRAELAPR
                     DIVARAIAAEIAKQTQDFVSLDISHQPAAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYSLGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSESLSSEAGIFSDGIQSAFSRPVLQAFNQRHLGILRNDTGLRC
                     AIAQLRLWKQNQTEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(2219..2884)
                     /gene="pgmB"
                     /locus_tag="ACFNML_001536"
     CDS             complement(2219..2884)
                     /gene="pgmB"
                     /locus_tag="ACFNML_001536"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFC6392890.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYLEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(2897..5155)
                     /locus_tag="ACFNML_001537"
     CDS             complement(2897..5155)
                     /locus_tag="ACFNML_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HFC6392891.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALSGSQTDWQVSNSFEVEVGGTPKTFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVVFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDEGYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPYDEELGVFVQHDGFLDKGIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPGAWTGYAFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLDGSHAAALEK"
     gene            complement(5389..6399)
                     /locus_tag="ACFNML_001538"
     CDS             complement(5389..6399)
                     /locus_tag="ACFNML_001538"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HFC6392892.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     TYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDDLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRRRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLNWPEFGNIRLNKGDTREATIAYGIES
                     LS"
     gene            complement(6403..7758)
                     /locus_tag="ACFNML_001539"
     CDS             complement(6403..7758)
                     /locus_tag="ACFNML_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HFC6392893.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLLYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKGY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLVITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMLGGLQATMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
BASE COUNT         1735 a         2225 c         2163 g         1669 t
ORIGIN      
        1 tgccgcgacg catcatctgc cagctaaggt aaattatacc ataaatttta aataaatcaa
       61 gcggtaagtg attttccacg gccactcgga ttaatccggg tggcttcctt tttaaaggtt
      121 tgcaaggcag gcaggggcgt ccgttttggc tgaaaataat atatcgattg cgcttcaagg
      181 ctctgcgcgt acctcattgc cgcccgttta aacacgtggt ttatctgaca ggcgcgcaat
      241 ccgccctctc atttaccgaa caagcggact agattcccgc ctgcgcggga atgacggcaa
      301 ccggccggtt acgtatcaaa aaataaagta attcggctag atatagtgga ttaacaaaaa
      361 tcaggacaag gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcacttggtg
      421 cttcaacacc ttagagaatc gttctctttg agctaaggcg aggcaacgcc gtactggttt
      481 aaatttaatc cactataccg tttcagacga tctctcaaac aaggacaaca ccatgcaaac
      541 cgattgcgac gtattgattg ccggaaacgg gctggcggca ctgacgctcg ccctgtcgct
      601 gcctgaatcg ttccgcatcg tcattttgtg caaaaaccgg ctggacgaca ctgccagccg
      661 tcatgcgcaa ggcgggattg cggcggcgtg gtcgggagag gacgacatcg aaaaacacgt
      721 tgccgatacc ttggaggcgg gcgcgggttt gtgcgacgaa gccgccgtcc gcaccatcct
      781 gtcgcagggc aaactggcaa tcgaatggct gctggcgcag ggcgtggcgt tcgaccaaaa
      841 taataacggc ctgcacctga cgcgcgaagg cgggcacacc tgccgccgaa tcgcccacgt
      901 cgccgactac acgggcgaag ccgtcatgca gagcctgact gtccaaatac gctgccgccc
      961 gaacatccgc gtttgcgagc agcagatggc gttggacatc caaaccgaat caggtgcggc
     1021 gtgcggactg accgtcctcg actgccgaac gcaagcaacc tacagcatcc gcgcccgcca
     1081 taccgtactc gcaggcggcg gcttgggaca gatttacgcc gccaccacca cgccgcccga
     1141 atgcacgggc gacgccatcg ccatggcgat acgcgcaggc tgcgcagtcg aaaacctcga
     1201 atttatccaa ttccacccca caggcttggc aagatcgtct gaaaacggcc gcaccttcct
     1261 gatttccgaa gccgtgcgcg gcgaaggcgg catcctgacc aaccaagcgg gcgaacggtt
     1321 tatgccgtat tacgaccgcc gcgccgaact cgcgccgcgc gacatcgttg cccgcgccat
     1381 cgccgccgaa atcgccaaac aaacgcaaga cttcgtctca ctcgatatca gccatcaacc
     1441 cgcagcgttc gtccgccggc atttcccgtc catccatcgg cactgtttat cccaatgcgg
     1501 cctggatatc acgcgccaag ccatccccgt ccgccccgtg caacactaca cctgcggcgg
     1561 catccaaacc gacccctgcg gcagaacttc cctgccgcag ctctactcct taggcgaaac
     1621 cgcctgcacc ggcttgcacg gagccaaccg ccttgccagc aactccctgc tcgaatgtgt
     1681 cgttaccgcc agacttgccg cccaaaccat cgcagacgga caagcattcc aagccaaaac
     1741 gtctccaagg ccgtctgaaa gcctctcctc cgaagcaggc atcttttcag acggcatcca
     1801 aagcgcattc agccgccccg tcctgcaagc gttcaaccaa cgccatctgg gcatcctccg
     1861 caacgatacc ggtctgcgct gcgccatcgc ccagctgcgg ctttggaaac aaaaccaaac
     1921 cgaaccgcac accgcgtccg aatacgaaaa ccgcaacctg ctcgaatgca gcctcgccgt
     1981 cgcccaagcc gcatacaggc ggagacagaa catcggcgcg cattttaata gtgattgtta
     2041 aggttggctg gttagaaaaa aaatcgttca aataaatttg aatagcatgg ttaagaaatt
     2101 ggcatttagt ttttagggtt gcgtaagttg ggttatcaat ctgttctaaa agcagaaagg
     2161 ccgtctgaaa cactgttcag acggcctttt acttccttga cactttatct gacgcgtttt
     2221 acctgcccga ctgtgcccac acgttttgca ggtaggcgta ggtcagctcg gcggtatcgg
     2281 acaccagcgc gatgtcgctg cccaagtctt cggctttgcc cacgccgata ggcaaggcgc
     2341 cggcggcttt gatggcggcg acacctgcgg cagcgtcttc aatgccgatg cagcggcgga
     2401 tgtccgcgcc cacgccctcg gcggcggcga ggaagatgtc gggggcgggt ttggaatgcg
     2461 cgacggcggc agggtcggcg acggtgtcga agaagtgggt cagccccatg cgttcgagca
     2521 ggaaggggcc gtttttactg gcggacgcaa gggcgatttt tttgccgttt gcttttaatg
     2581 cttccagcag cggcaaaatg ccaagataca cgtcttcggg tttgactgcc tgtatcatct
     2641 ccaagtagtt gtcgtttttg cggcgggtca gttcggcgaa ctcggcttcg ccgacggttt
     2701 taccgccgtg cgcgaggatg cgtttgagcg aatcgtcgcg cgacacgcct ttgagctgct
     2761 cgttaaactt gcggtcgatg ccgatgccca gttcttcggc gagttttttc cacgcgcggt
     2821 agtggtattc ggcggtgtcg gtgatgacac cgtcgagatc gaagaggact gcggtaaacg
     2881 tcattttgca ccctccttat ttttccaacg cggcggcgtg gctgccgtcg agcgtgatgt
     2941 ctttgccata cacctgcaaa tcgagcggct cgcctttgag cagggtgaag acgacgtttt
     3001 ctttgccgac ggcgacttta atcagacggc ctcggtagtt gatatggaag gcgtagcctg
     3061 tccatgcgcc cggcaggaac ggtgcgaagc tgagtttacc gccccaagtt ttcatttggg
     3121 cgaaaccttg gacgatggcg agccacgagc cggtcataga agtaatgtgc aggccgtctt
     3181 cggtgtcgtt gttgtagttg tccaagtcca ggcgggcggt gcgctggtac atttccacgg
     3241 ctttttcttc tttgcccagt tcggcggcga gaatagagtg gatacaaggc gacaacgagc
     3301 tttcatgcac ggtcatcggt tcgtagaagt cgaagttgcg gcgtttttcg tcgatattga
     3361 aacggtcgcc gaagaaatag atgccttgca atacgtccgc ctgtttgata aagggcgaac
     3421 gcaggatttt gtcccacgac catttctggt tgagcggcaa atcgtcgggc gaaagcgcgg
     3481 acacggggcg gatgcctttg tcgaggaagc cgtcgtgttg cacgaatacg ccgagttctt
     3541 cgtcatacgg acggtacata ttcgcgctga tgtccgccca tttttccaac tcggcggcgc
     3601 tcacgttcaa atccggacgc gggtatttcg ccagggcttc gcgggtgtag tccaataccc
     3661 atgcggcgag ggtgttggtg taccagttgt tgttgatgtt gttttcgtat tcgttcgggc
     3721 cggttacgcc gtgaatcatg tatttgccgc tgcgtttgga gaagtggacg cggtccgccc
     3781 agaagcggga cacttcgacc aagacttcca agccttcttt ggcaagatag ccctcgtcgc
     3841 cggtgtagtt ggtgtagttg tagatggcgt aaggaatcgc gccgttgcgg tggatttcct
     3901 cgaaggtgat ttcccattcg ttgtggcact cgatgcccgt aaacgttacc atcggataga
     3961 gtgcgcccgc caagccctgt tcgcgcgcgt tgtgttgcgc ctgtggcagt tggttgcggc
     4021 ggtattgcag caggttgcgg gtaacttcgg gttccgccag tgcgaggtag agcggcacgg
     4081 cgtaggcttc ggtgtcccaa taggtcgcgc cgccgtattt ttctccggta aagcctttcg
     4141 ggccgatgtt cagacgcgcg tcttcgccgt agtaggtgga gaacagttgg aacagattga
     4201 aacggatgcc ttgctgcgct tcgtcgttgc cttcgatgac cacgtcggcg atttcccaac
     4261 ggtgcagcca gcctgctttg tgcgcgtcca gcaaggtttc aaacacaacg cctgcgactt
     4321 tttccgacaa ggcgcggcct gcggctttca ctgcttccaa gccctgataa tcgcggctgg
     4381 tggtaacaat aacccgtttt tcaaaggttt tgggcgtgcc gccgacttcg acctcgaaag
     4441 aattggagac ttgccagtcg gtttggctgc cgctgagggc tttgaagctg ccggcgaaag
     4501 tttgctcggc gttgacgata aattgttcca cgccgaaagg gttggcaacg gtttgggcgg
     4561 caatgtagga gagactgtct gaaacgcctt tgtccaatac ctgccagaat ttttcttcgt
     4621 agttggagtc ttcgtttttc acgtcggcat cgatgatgga atcgatgcgg acttggtggg
     4681 ttttgccgtc aacggatacg gcttcccagc ggataaccgc cagctctttt tgcgcgacgg
     4741 acaggaattt gcacacattg aaacgcacac cgaatacggt gaacgagcgg cgcaacacgc
     4801 cgtgctgcat atcgagttcg acggagaagc cggcaacatc gtttttcgcc aagtccacct
     4861 cttgtccgtc gacaaagatt ttgactttgc tgaaattgag cgcgttgatg gctttaccga
     4921 aatatttggg gtagccgttt ttccaccagc cgacgcgggt tttgtcggga aaccacacac
     4981 cggcgatgta ggtacccaag tggctgtcgg cggaataggt ttcctcaaag ctgccgcgca
     5041 tacccatata gccgttgccc aaactggtca ggctctcttg cagccgtttg tgttcttttt
     5101 ccagctttgc cgaacgcagc gtccaagggc tgatttccat aattcttgtg tacatttatg
     5161 aagctcctgt ttggattgat ttgagggaat ggtgaaatct tatagtggat taacaaaaat
     5221 caggacaagg cgacgaagcc gcagacagta cagatagtac ggaaccgatt tacttggtgc
     5281 ttcagcacct tagagaatcg ttctctttga gctaaggcga ggcaacgctg taccggtttt
     5341 tgttaatcca ctataaaaag gccgtctgca accggtgtta ggaagctcct aagaaaggga
     5401 ttcgatgccg taagcaatcg tcgcctccct agtatcaccc ttgttcagac gaatattgcc
     5461 gaactcgggc caattcaggc tgtcgggcag cgtctgcgcc tcggtcgcca gcgcgtcgta
     5521 aacgcccgca tcgtgccgcg cgaaatcctg cggggcggcg gtaaagatga ccaagccgtt
     5581 gcggtcgctg tatatgctga tacgacggcg gcgtccggct tgcaacacag cggcgggacg
     5641 gcctatatcg gacggcacgc ggtaagcgtc gtcaaaaccg gcccgacccg tttcgcggcg
     5701 cagggcggca acggcggcat ccagcggctt gggccggctg aaatcaaata cttcgaggtc
     5761 gtctgaaacc gttgagacgg gcagtttttc ggcatcggcc ggaatatgtc cgccctgcgg
     5821 aatatgcaga accgcatcgt gcaggcccgc gtccagccgc cagtaaatgt gcagcgtcgg
     5881 gtcgaacacc gtgtcgccga gcgcggtggc gcgataggta acggtaagcc ggtcgtcctc
     5941 gtccaagcgg taggaaatat ccaaatccaa atcgttggga taaccgtcgg ccgactgttg
     6001 caggcggctg cgcagcacca ccgaacggcc gtctgccgcc accgcgttga aacgggtaac
     6061 ggccagcccg tgcgaaccgc cgtgcagcgc gttcctgcct tcgttggcct ccacgcggta
     6121 agtcctgccg ttgatgtcga acgccgcacc gcggatgcgt ccggccacgc gccctatctg
     6181 cttgttaatc tgaaacggat tgtccgcata ggaagccgca tcgtcgaacg acaccacgag
     6241 gttttcgcgc acgccgtctg ccaaaacgga aaattcctgc acaatcccgc ccaagtccag
     6301 cacgcagaca cgcgtaccac gccggttgga cagcacatag ccggttacgg cacgcccgtc
     6361 gatcaggccg aaatcgcggg tagcgggggt atcgctcatc gctcaaaccc cgccgtgtgt
     6421 ttctttaatc aggaacacgg aaaacgcgcc cagcagcagg acgacgcccc ctaccaagaa
     6481 catagtggcc tgcaagccgc ccagcatagg gaaaagcacg aaactcaaca gcgaagcgac
     6541 gatttgcggc atacagatag agccgttaaa caggcccaag taagtgccca tatgcttgcc
     6601 cgacaaggcg ttggttacaa tcgtcagcgg ataagtgata atgcccgccc aagcgatgcc
     6661 gattaaggta taagacaaca ccagcgcgta ttggttgccg atgaagaaaa cggagaaaaa
     6721 gccgagcgcg cccaaagcca aacagccgaa ataacccgcc ttatggtatt tattcggcac
     6781 tttcgccaat acaaacgaac aaatcaccgc cgcaaccgac tgcaccgccg ccaaaacgcc
     6841 gtaccagttg cccgcctcct gataacctac ggaagacgca tcggtggtgt gccagacgtt
     6901 ttccgcaatc gcgcctgccg agtaagtcca catatattgg aaggcgaacc agcagaaaaa
     6961 ctgtaccaga gtaaccgtcc aaaacgcttt aggcgcggtt tttaagagtt cgaaccagtt
     7021 ggctttttcc tgattcgcgg cgacatcgat gccgtggtaa cgggcgtagg tttccggatc
     7081 gtattccttc actttgaaaa tcgtgaacgc gctggtaatc accagcaacg ccgcacccac
     7141 ataaaacgcc acgaccacgg tctgcggcac aacgcctttc tcggcggtgt tcgccaaacc
     7201 gatatacgca aacacaaacg gcagaatcgc cgccacgacc gcgcccgtat tcgctaagaa
     7261 actttgaatc ccgtaggcgt agcctttctg ctcctcgttg accatgtcgc cgaccatcat
     7321 cttaaacggc tgcatcgcca tatttgacga cacgtccaac agcgcaatca tcagcgcgcc
     7381 gaacgacaaa gccgccagcg acgcatagcc gaaaccgaag ctgcccgagt tcggcatcaa
     7441 aatcatcaca ataaccgcaa tcagcgtgcc ataaagcaga tacggcagac ggcggccgcc
     7501 caaacgcggc ttccaagtgc ggtcggagta atggccgaca atcggctgca ccagcatccc
     7561 cgccagcggc ggcaggatga aaaaccagcc caaattgtgc gggtctgcgc ctagcgtttg
     7621 aaaaatgcgg ctcatttgcg agctttgcag ggtaaaggcc gtctgaacgc cgagaaagcc
     7681 gaaactgagc atccaaatcg tgctttttgc cagcgcgggc aaaccttgtt ttgctgtttg
     7741 aggcgtatat tccgacataa ggtaaatcct tttttgattt gaaaagtata gt
//
LOCUS       DAXHTR010000104         7688 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.104, whole genome shotgun sequence.
ACCESSION   DAXHTR010000104 DAXHTR010000000
VERSION     DAXHTR010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7688
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.104"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(190..468)
                     /locus_tag="ACFNML_001540"
     CDS             complement(190..468)
                     /locus_tag="ACFNML_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392894.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSPKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
     gene            complement(475..693)
                     /locus_tag="ACFNML_001541"
     CDS             complement(475..693)
                     /locus_tag="ACFNML_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFC6392895.1"
                     /translation="MKFINTCRKYGAKLAVVTAAPLALAAHANATLPDTAKNALEAAK
                     ADGMEAGWIVVGVFAALFVFSIVKRVMK"
     gene            complement(767..964)
                     /locus_tag="ACFNML_001542"
     CDS             complement(767..964)
                     /locus_tag="ACFNML_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392896.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
     gene            complement(969..1258)
                     /locus_tag="ACFNML_001543"
                     /pseudo
     CDS             complement(969..1258)
                     /locus_tag="ACFNML_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1303..2646)
                     /locus_tag="ACFNML_001544"
     CDS             complement(1303..2646)
                     /locus_tag="ACFNML_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HFC6392897.1"
                     /translation="MEDKQGMTKAVVGVMTDALADGRKPATASNLPPLSNRGGTETET
                     AGRVQEVFECYETYITDGKGNLLGVPLRRGVSDSAFIDQISFSFHEKTFFDKYGVRVS
                     LLEDEDFIRAASMLAEEVFGFGIYKESKGSGGRFYERCWLMGSEDVLYGRVHFGGQNN
                     TILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDIAKDFFNGEYSPNQAREDRN
                     KGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSRESSKYVRVYEKGKQLGDKT
                     STWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERFVQKATRIHAVKEDKVISAD
                     RYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHKLPKSLAPEAYDCAFLKAQA
                     IHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESYNNFIYDKFARLPISWA"
     gene            complement(2731..3243)
                     /locus_tag="ACFNML_001545"
     CDS             complement(2731..3243)
                     /locus_tag="ACFNML_001545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392898.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPINQILNHIHPARYAVTFENNVDSKLRRHWVAG
                     ATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAILCNKERLTSSEPLSDFVL
                     SVLKPYIFRP"
     gene            complement(3234..3587)
                     /locus_tag="ACFNML_001546"
     CDS             complement(3234..3587)
                     /locus_tag="ACFNML_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392899.1"
                     /translation="MFTTNDLRHFLEGLAILFSIGYWGTMLLLLWFLVRFAYKKPKRN
                     PGKILSGSLTAAAILMLFVWIIPKQFGPIKEEIQAQEEWDRKYKEAEAVFNEQCKTAG
                     ERFTRRRTMWKGLCC"
     gene            3823..5250
                     /locus_tag="ACFNML_001547"
     CDS             3823..5250
                     /locus_tag="ACFNML_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HFC6392900.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     AVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQSRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            5319..6722
                     /locus_tag="ACFNML_001548"
     CDS             5319..6722
                     /locus_tag="ACFNML_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HFC6392901.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTDLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(6886..7673)
                     /locus_tag="ACFNML_001549"
     CDS             complement(join(6886..7418,7418..7673))
                     /locus_tag="ACFNML_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HFC6392902.1"
                     /translation="MSQTAATFNLSRNTLYLWIRLKKQTGSLKHQVTGLNAVKSDRQK
                     PAQYIGQHQDAYLHEIAKHFDCTAATICYALKQMGITRKKRPTTYKEQDPAKVTHYLT
                     QPAEFSDYQRVYLDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQVGNRL
                     IAPMVYQNTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVL
                     PLAPYSPELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1913 a         1827 c         1917 g         2031 t
ORIGIN      
        1 ttgctagacg gctgatattt ataaagtgcc aactgcgcct gagtgataaa cggcttgttc
       61 atggttctgc ctttcaatga ttgttttgaa agcctgattt tgacaccata acttcatgcg
      121 ctcaattctt aaacagaacc gccccgatta atacgggtac ggaaacgccg agataaaaat
      181 aaaaatccat catttcaaaa cctttttcag cagggaaaca aagtaaacgg acgcgaggat
      241 gccgaatact atccaacctg tttcaagacc gctttgcagg ttgtctttcg gactgcattc
      301 cgccaataaa agccttagcg gctgaccgtc cgacatcttc cacaggctgc cgttatattc
      361 cggcctgaca atctgtccgt tttctttgat tcttggtact accaagctga aataaaggtt
      421 ttcagcctgg tgcttctcaa gacatttatt tccgacttgg tagtacatgc cgtcttactt
      481 catcactctc ttaacgatgg aaaacacaaa aagcgcggcg aaaacgccaa cgacaatcca
      541 accggcttcc ataccgtccg cttttgcggc ttccaaagcg ttttttgccg tatcgggcaa
      601 cgttgcattt gcatgtgcgg ccaaagccag gggagcagct gttacaacag ccagttttgc
      661 gccgtattta cggcaggtgt taataaattt catgatattt tcctttaaaa agtgtttggc
      721 ggtaatggat ggagcgtttt tcagacgacc gccgaacatc cgaaaatcag tctttcaaaa
      781 atccgaatac gacaaattcg tattggttgc cgatttcttc caaacctgcg ttaatcgctt
      841 cttcgaagtc gtagaaataa tcggcattgg tgattaattt ggtatgtccg atgtcgcccg
      901 tttcaggaga gagatacaga aagtcccctg ttgatacgga ctggacaaca tagactttct
      961 gcattcaatc agcctttctt aacgggttga aaaccgatga ctttcagttt ttgggttttg
     1021 cccgtagtga cgatttctac gttcaggttt gcttcgatcg gaaattgggc gtttcggaac
     1081 tgctcgaaat tggcagagcc gccgaaatcg tattcagtag tagagctgcc caatgcgttg
     1141 ccttgggagc tgtctaaggg tgtggcgaca atcaggcggc aatagtcgaa gctcttgcct
     1201 tcgatttgtc cgttgaattt ttaacgccga cgatgtggcc ttgaagttgg atgttcattt
     1261 tttggtttcc ttgtgtgatt aaacgtcttt cgggcagaca ctttaagccc atgaaatcgg
     1321 tagtcttgcg aatttgtcgt aaatgaagtt gttatagctt tcttcattgt tgacgtgttt
     1381 ttgctgttca agctgttttt caagattctc gtaatattcg tacatatagt aagggtcttt
     1441 gtacggtttg aatgcgggct gttcatgaat ggcttgagct ttcaaaaagg cgcagtcgta
     1501 ggcttcggga gccaaagact tgggcagctt gtgatgactc ggctcaatca gttcaaacag
     1561 tttggctttg tccaattcgg gaaaaatgaa tttcagaccg tttgccgcac gtccaaactg
     1621 tttttttacc cattcaaggt agcggtcggc tgaaatgacc ttatcttcct taaccgcgtg
     1681 tatgcgcgtt gccttttgga cgaatcgttc gcaaatcgga tatgcgccgc cgaaatattc
     1741 gcccggattc tgcaaaactt caaaagggat aacgatgtct tttgctttga attcaatttc
     1801 aaagcgcgtc catgtgcttg ttttatcgcc caactgcttg cctttttcat agacgcggac
     1861 atatttggac gattcacggg agccgatacc ataggtcttg cctttggtca ttttggcttc
     1921 atcgtcttct tcccaatctg accccaaaca ttcgcctttt ggtttgacgt gatgacaggt
     1981 aaacatacct ttatttcggt cttcacgggc ttggttcggg ctgtattcgc cgttgaaaaa
     2041 gtcttttgcg atgtcaacgc gtgtgatttt tgggcggatt gcattagtca ggaatgcgaa
     2101 aagtcgtgat tcccagcctt cttttgcgac gccgcaaccg gtgccggtca gttcgaaaag
     2161 gatggtattg ttttgtccgc caaaatggac gcgaccgtat aggacgtctt ccgaacccat
     2221 caaccaacag cgctcataaa aacgaccgcc cgagcctttg gactctttgt agataccgaa
     2281 accgaaaact tcttcggcga gcatggacgc ggcgcgaatg aaatcttcgt cttccaaaag
     2341 acttacacga acgccgtatt tatcgaaaaa ggttttttca tgaaatgaaa agctaatttg
     2401 atcaatgaaa gccgaatctg atacaccgcg ccgaagagga acgcctaaca ggtttccttt
     2461 accgtccgtt atgtacgttt cgtaacattc gaagacttcc tgaaccctgc ctgccgtttc
     2521 ggtttctgta ccccccctgt tagataaggg gggaagattt gaagcggttg ccggcttcct
     2581 gccgtccgct aacgcgtccg tcatcacgcc gacaaccgcc tttgtcatcc cttgcttatc
     2641 ttccatggtg cgaatcctca aaaacgggca aaaaaaagcc ctgttacttg tagaaagtaa
     2701 agggcgttaa tttttgttaa tcgtcccttc ttagggacga aatatataag gttttaaaac
     2761 gctaagaaca aaatccgata acggctctga agaagtaagt ctttctttat tgcacaagat
     2821 agcaaacttc cacggcattc tcgccccacc cgtgccgtcc aagccttttt caaagacata
     2881 gatggttttc ttggcaatca cttcgtcagt ttggcggtcg ataatccgta tggtcgcacc
     2941 tgccacccag tgcctgcgca gcttggaatc gacattgttt tcgaacgtta ccgcataacg
     3001 ggcggggtgt atatgattaa gtatttgatt tatcggaaaa tctttacctg aataccgaat
     3061 aatatcggaa tggttgggtt gcaggacatc cacatagcgg tattccccat cgctaaaaaa
     3121 tcctaggaaa cgagcaataa aatttacgcc ttcgctggtc tgtaaagccg cattgtccca
     3181 catcgggtct ctggtttttg catctgccga aacggtacgc tcaggtacta ccttcaacag
     3241 cataatccct tccacattgt ccgccgtctg gtaaatcttt cccccgccgt tttgcattgt
     3301 tcgttaaaca cggcttcggc ttctttgtat tttctgtccc actcttcttg tgcctgtatt
     3361 tcttctttga tcgggccgaa ttgtttggga ataatccaaa caaacagcat caggatagcg
     3421 gcggcggtca ggctgcctga aaggattttg ccggggttcc gtttgggctt tttataggca
     3481 aagcggacga gaaaccaaag caacagcagc atggtgcccc aatagccgat tgagaatagg
     3541 atggccaaac cttctaggaa atggcgtaaa tcgtttgtgg taaacatggg ttgttcctgt
     3601 ggttaaatgt gcaggctgct ttttgccgaa ccttgccgca tctcaaaagc agcctgcgct
     3661 tcagcgttgc gttacgcagt aaaataatga atatttgtaa cgacttgggt attttttgtc
     3721 aatattcccg cctttccctt aacagctgcc gcgctttccg ttaaaattcc tttacatatt
     3781 tatattgttt cctgtttcta tattgccaag gttatacccg ttatgttttt ctccgccctg
     3841 aaatcctttc tttctcgata cattactgta tggcgcaatg tttgggcggt gcgcgaccag
     3901 ttggaaccgc ccaaacgcac ggcggaagaa caggcgtttt tgcccgcgca tttggaactg
     3961 accgatacgc cggtctctgc cgctccgaaa tgggcggcgc gttttattat ggcgtttgcg
     4021 cttttggctt tgttgtggtc ctggttcggc aaaatcgata ttgtggcggc ggcttcgggc
     4081 aaaacggtgt cgggcgggcg cagcaaaacc atccagccgc tggaaacggc ggtggttaag
     4141 gcggtacatg tgcgcgacgg gcagcatgtg aaacagggag aaacgctggc ggaactggag
     4201 gctgtgggaa cagacagcga tgtggtgcag tcggagcagg ctttgcaggc tgcccaattg
     4261 tccaaactgc gttatgaagc ggtattggcg gcattggaaa gccgtaccgt gccgcatatc
     4321 gatatggcgc aagcacggtc tttaggtctc tccgatgccg atgtgcaatc ggcgcaggtg
     4381 ttggcgcagc accagtatca ggcatgggcg gcgcaggatg cgcaattgca gtcggctttg
     4441 cgcggccatc aggcggaatt gcagtcggcc aaggcgcagg agcagaagct ggtttcggtg
     4501 ggggcgatcg agcagcagaa aacagcagac taccgccgtt tgcgggccga caattttatt
     4561 tcggaacatg cgtttttgga gcagcagagc aaatcggtca gcaattggaa cgatttggaa
     4621 agtacgcgcg gtcagatgag gcagattcag gcggccattg cacaggcgga gcagagtcgg
     4681 gtgctgaata cgcagaacct gaaacgcgat acgctggatg cgctgcgcca ggcaaacgaa
     4741 cagattgacc aataccgcgg ccaaacggat aaggcaaagc agcggcagca gctgatgaca
     4801 atacagtcgc ctgcggacgg cacggtgcag gaattggcca cctatacggt gggcggtgtg
     4861 gtgcaggctg cccaaaaaat gatggtggtt gcgcccgatg acgacaaaat ggacgtggaa
     4921 gttttggtat tgaacaaaga catcggtttt gtggaacagg gacaggatgc ggtggtgaag
     4981 attgagagtt ttccctatac gcgctacggt tatctgacgg gcaaggtgaa aagtgtcagc
     5041 catgatgcgg taagccacga acagttgggc ttggtttata cggcggtggt gtcgctggac
     5101 aaacatacct tgaatattga cggcaaagca gtgaatctga cggcgggcat gaatgtcacg
     5161 gcggagatta aaacgggtaa acggcgggtg ctggattatc tgttaagccc gctgcaaacc
     5221 aaattggacg aaagctttag ggagcgatag gcggatccgt actgggcatt tgttatccgc
     5281 cggttcggac atgcagactg cctgaaacca ttgcccggat gacattgctc aatctaatga
     5341 taatgcaaga ttacggtatt tccgtttgcc tgacactgac gccctatttg caacatgaac
     5401 tattttcggc tatgaaatcc tatttttcca aatatatcct acccgtttca ctttttacct
     5461 tgccactatc cctttcccca tccgtttcgg cttttacgct gcctgaagca tggcgggcgg
     5521 cgcagcaaca ttcggctgat tttcaagcgt cccattacca gcgtgatgca gtgcgcgcac
     5581 ggcaacaaca agccaaggcc gcattccttc cccatgtatc cgccaatgcc agctaccagc
     5641 gccagccgcc atcgatttct tccacccgcg aaacacaggg atggagcgtg caggtgggac
     5701 aaaccttatt tgacgctgcc aaatttgcac aataccgcca aagcaggttc gatacgcagg
     5761 ctgcagaaca gcgtttcgat gcggcacgcg aagaattgct gttgaaagtt gccgaaagtt
     5821 atttcaacgt tttactcagc cgagacaccg ttgccgccca tgcggcggaa aaagaggctt
     5881 atgcccagca ggtaaggcag gcgcaggctt tattcaataa aggtgctgcc accgcgctgg
     5941 atattcacga agccaaagcc ggttacgaca atgccctggc ccaagaaatc gccgtattgg
     6001 ctgagaaaca aacctatgaa aaccagttga acgactacac cgacctggat agcaaacaaa
     6061 tcgaggccat agataccgcc aacctgttgg cacgctatct gcccaagctg gaacgttaca
     6121 gtctggatga atggcagcgc attgccttat ccaacaatca tgaataccgg atgcagcagc
     6181 ttgccctgca aagcagcgga caggcgcttc gggcagcaca gaacagccgc tatcccaccg
     6241 tttctgccca tgtcggctat cagaataacc tctacacttc atctgcgcag aataatgact
     6301 accactatcg gggcaaaggg atgagcgtcg gcgtacagtt gaatttgccg ctttataccg
     6361 gcggagaatt gtcgggcaaa atccatgaag ccgaagcgca atacggggcc gccgaagcac
     6421 agctgaccgc aaccgagcgg cacatcaaac tcgccgtacg ccaggcttat accgaaagcg
     6481 gtgcggcgcg ttaccaaatc atggcgcaag aacgggtttt ggaaagcagc cgtttgaaac
     6541 tgaaatcgac cgaaaccggc caacaatacg gcatccgcaa ccggctggaa gtaatacggg
     6601 cgcggcagga agtcgcccaa gcagaacaga aactggctca agcacggtat aaattcatgc
     6661 tggcttattt gcgcttggtg aaagagagcg ggttagggtt ggaaacggta tttgcggaat
     6721 aaagcaggct gaaacggtta tgaaattccc aaagcagcct gcaccccgtt tcgaaagtgc
     6781 aggctgcttt gggattgatc cgatactttc acattctcat tgcctttaaa taaagcacag
     6841 ttaaaaacac atttaaatta agtgcgaatt aattcaaatg tatattcaat taaaatcaaa
     6901 ataggacagt agtgcatcgt caaatcgggc gtaatcagac aaaacggttc gcagataccg
     6961 cttaatattc gcccacactt tctcaatcgg gttgagctca ggtgaataag gtgcaagagg
     7021 caatacctta tgtcccaatt tttccgccat ttcccgtaag acacccatac ggtgaaatcg
     7081 cgcattatct aaaataatca ccgatttttg agtcaatgcg ggcagtaggc attgctgaaa
     7141 ccacgcttca aaaaagactc cggtcatcgt attttgataa accatcggag caatcagccg
     7201 gttgccgact tgtgcggaca ccaaagataa gcgttggtat ctttttccac ttatctgcgc
     7261 tttcactatt tgccctttca ggctgcgggc atagggacgg aacaggtagc ggtcaaatcc
     7321 tgtttcatcc aaataaacac gttggtagtc ggaaaattcg gccggctgtg tcaaataatg
     7381 cgttactttg gccgggtctt gttctttgta agtggtggtc tttttttgcg cgttatcccc
     7441 atctgtttga gtgcatagca aatggtggct gccgtacaat caaaatgttt ggcgatttca
     7501 tgcagatagg catcctggtg ttgcccaata tattgagccg gtttttgcct atccgatttg
     7561 acggcattta gaccggtaac ttgatgtttt aggctgcctg tttgtttttt aaggcgaatc
     7621 cacaggtaaa gcgtgtttct tgacaagtta aacgttgctg cggtttggct gatgtttttg
     7681 cattgttc
//
LOCUS       DAXHTR010000105         7686 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.105, whole genome shotgun sequence.
ACCESSION   DAXHTR010000105 DAXHTR010000000
VERSION     DAXHTR010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.105"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(45..461)
                     /gene="dksA"
                     /locus_tag="ACFNML_001550"
     CDS             complement(45..461)
                     /gene="dksA"
                     /locus_tag="ACFNML_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFC6392903.1"
                     /translation="MAKLTEQDILNWSGPEDDYMNDDHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(608..1417)
                     /gene="proC"
                     /locus_tag="ACFNML_001551"
     CDS             complement(608..1417)
                     /gene="proC"
                     /locus_tag="ACFNML_001551"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFC6392904.1"
                     /translation="MNRVEIMNVYFLGGGNMAAAVAGGLVKQGGYRIHIANRGAEKRE
                     RLQKELGVETSETLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLPVDTL
                     SRYLGGTRRIVRVMPNTPGKIGLGVSGMYAEAEVSETDRRIADRIMKSVGLTVWLEDE
                     AQMHSITGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVTLAEQTGE
                     DFEKLQKNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1448..1855)
                     /locus_tag="ACFNML_001552"
     CDS             complement(1448..1855)
                     /locus_tag="ACFNML_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HFC6392905.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCQLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQGGFDCLEKQGLRRNGLSERVRW"
     gene            complement(1855..2541)
                     /locus_tag="ACFNML_001553"
     CDS             complement(1855..2541)
                     /locus_tag="ACFNML_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HFC6392906.1"
                     /translation="MTVLQERYREVSDRIGKLVLQAGRKPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAVKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLADLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2661..3704
                     /gene="pilT"
                     /locus_tag="ACFNML_001554"
     CDS             2661..3704
                     /gene="pilT"
                     /locus_tag="ACFNML_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFC6392907.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNTGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3867..5093
                     /locus_tag="ACFNML_001555"
     CDS             3867..5093
                     /locus_tag="ACFNML_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HFC6392908.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVAPRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5379..7529
                     /gene="yccS"
                     /locus_tag="ACFNML_001556"
     CDS             5379..7529
                     /gene="yccS"
                     /locus_tag="ACFNML_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFC6392909.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQENVANAYEALGSYLEAKADFFDPDEAEWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKIAERLKSGETADDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1884 a         2551 c         1683 g         1568 t
ORIGIN      
        1 ctgtctgaaa cttagggctt cagacggcat tttgtttgcc gccatcagtc ggcaaactgt
       61 tttttcatcc tctctcggcg ttcttgggac tcaacagata aagtggctgt cgggcgtgcc
      121 agcagccgct tcagaccgat aggctctccc gtatcggcac agaatccata atccccttca
      181 tcaatattgc ggatggtcgc ctgtatttta ctgagaagtt ttcgttcccg atcgcgggta
      241 cggagttcca atgcgtactc ttcttcctgt gtggcacggt cggcaggatc gggggctgat
      301 tcgtgttctt ggagatgccc tgtcgtagcg gaagcatttt cgatgagttc gtcttgcatt
      361 tttaccagca attcgcggaa aaaagccaaa tggtcgtcat tcatataatc gtcttccggc
      421 ccgctccaat tcaaaatatc ttgttctgtc agctttgcca taatgtatat cctctcggtt
      481 caccgaaaaa aacaaagcac cacaaagtgg ctttattcct ttgattttta taaggcttat
      541 tgcctaaggc gtttttgaaa acgcgcatca taacacgttt tggattggtt tttttatttt
      601 ctttacatta ttgatattgc cgttccattt cctgcgaacg gcgcacacag gcacaaacgc
      661 cctcacttat ggcttcggcg acacgatgcc gcctgaaggc ttccacggct tcgtgggttg
      721 tcccgccttt tgacgttaca tttttttgaa gcttctcgaa atcttcaccc gtctgctcgg
      781 caagggtaac cgctccttta aacgttgcca gactgagcgc gcgtgcttct gccatatcaa
      841 acccttgtcg gatggcggca ttttgcaatg cgtccagcag ataaaacaca tatgccggcc
      901 cgctgccgct gataccggta atgctgtgca tttgcgcttc atcttccaac caaacagtca
      961 aaccgactga tttcatgatt cgatcggcaa tcctgcggtc tgtttccgat acttccgctt
     1021 cggcatacat accggatacg cccagcccga tttttccggg tgtattcggc ataacccgga
     1081 caatgcggcg tgttcccccg aggtaacggc tgagcgtatc gaccggcaat ccggctgcga
     1141 cagaaagcac caatgcgccg ttggtgcgga tatttttgca cgcggcttcc atatcctgcg
     1201 gcttgacggc aaggattaaa acatcgtcgg aatgaagctc cggcaaggtt tcagaagttt
     1261 cgacccccaa ctctttttgc aaacgttcgc gcttttccgc accccggttg gcaatatgga
     1321 tgcggtaacc gccttgtttg accaatccgc ccgcaacggc agccgccata ttgccgccgc
     1381 cgagaaaata aacattcatt atttctaccc tattcaaaat tacctgacta aattcattcc
     1441 caatttttta ccatcggacg cgctcggaca gaccgttgcg ccgcaacccc tgcttttcca
     1501 agcagtcaaa tcctccctgt ttgaacgtct caatcaaagg tttgccgtca cgcgtacgcc
     1561 aatccagccc gcaggcttca aacttcccta ttttcctgta aaagtaagat tgttgtgcgg
     1621 attcatattc gtttgccctg acggaacgat tgccgtctga aagcggtatc tcgtagtcct
     1681 ctaaagacgg cggactctcg ccgccaatgt cccaaaaatc gatggcagcc ggttttctcg
     1741 gcttacacca gccggacagg gacgaacact cataccaacc cgccaactgg cagcctgcaa
     1801 ccgtaaatgc aagcaacgca gccattgccg tatattttat tgcccgaatt tccatcagcc
     1861 ccttttcccg aaaatcgcgc tgccgatacg gacgtgtgtc gcaccgcact caatggcggc
     1921 aggcatatcg tccgacatcc ccatagacag cacgtctgcc ttaacgccag ccgtattgag
     1981 gtcggcaagc agtttccgca tcgtttgaaa ttgcaccttc aactccgttt cactgctgtt
     2041 ggctttggca acacacatca gtccgcgtac gacgatattc ggcagcttcg ccacttccac
     2101 agcaagcgcg actgcttctt cgggcgcgac accgtgcttc accgcctcgc ccgcaatatt
     2161 cacctcgata cacacctgca agggcggcat tgaggaagga cgctgttcgc tcagccggac
     2221 agcggttttc agacggcata cggtatgcac ccaatgcgcg cgttcggcga caaacttggt
     2281 tttgttggac tgcacatcgc cgatgacgtg ccacacgatg tcggtcaaat ccgccaactc
     2341 ttccgttttg ccgtaccact cctgaatata gttctcgccg aaatcacgct gtccggcggc
     2401 gtaaacttcg cggatgccgt ctgaagggaa agtcttaccg acggcaatca ggctgacgga
     2461 atgcggcttc cttcccgcct gcagaaccaa ttttccaata cggtcggaca cctcccgata
     2521 acgttcttgc aacaccgtca tagattatcc cctaattaaa aatgattaaa cagttgaaac
     2581 ccctccagtc agggcggtac aatcaaggtt gttagaacca ttccaaccaa tcgaaacatt
     2641 atactaaaca gagccgcatt atgcagatta ccgacttact cgccttcggc gctaaaaaca
     2701 aagcatccga ccttcacctg agttcgggca tatcccctat gattcgggtt cacggcgata
     2761 tgcggcgcat caaccttccc gaaatgagcg cggaagaggt cggtaatatg gtaacttcgg
     2821 tgatgaacga ccaccagcgg aaaatctacc agcaaaactt ggaagtcgac ttctcgttcg
     2881 aactgcccaa cgtcgcccga ttccgcgtca acgccttcaa caccggccgc ggtcccgccg
     2941 ccgtattccg caccattccc agcaccgtct tatcgctgga agaattgaaa gccccgagca
     3001 ttttccaaaa aatcgcagaa tcgccgcgcg gcatggtatt ggttaccggc cctaccggtt
     3061 cgggcaaatc gaccacgctt gccgcgatga tcaactacat caacgaaacc cagccggcac
     3121 acatcctgac catcgaagac ccgatcgaat tcgtccacca aagcaaaaaa tccctgatta
     3181 accagcgcga gctgcaccag cacaccctca gcttcgccaa cgcgctgcgt tccgcattgc
     3241 gcgaagaccc cgacgttatc cttgtcggcg agatgcgcga ccccgaaacc atcggcttgg
     3301 cactgaccgc cgccgaaacc ggacacttgg ttttcggcac gctgcacacg accggcgcag
     3361 caaaaaccgt cgaccgtatc gtggacgtat tcccggcggg agaaaaagaa atggtgcgct
     3421 ctatgctgtc cgaatcgctg accgccgtca tctcccaaaa cctgctgaaa acgcacgacg
     3481 gcaacggccg tgtcgcctcg cacgaaatcc tgattgccaa ccccgccgtc cgcaacctca
     3541 tccgcgaaaa caaaatcacg cagattaact ccgtcctgca aaccgggcag gcgagcggta
     3601 tgcagacaat ggaccaatcg ctgcaatcgc tggtgcgcca agggctgatc gcaccggaag
     3661 tcgcacgcag acgcgcgcaa aacagcgaaa gtatgagttt ctgacacaca accgccttcc
     3721 ggccataccg gcgggaaaac aaggcgcaaa cacgcggggc gggacgcagc atcccgcccg
     3781 gctacctttc cgaacaagaa gcgtccgcct tcctgttgaa acctgccgcc gcaaactgca
     3841 aggcttaaac cgaaaagaag ctaacgatga ataccgataa cctgcacgac atcttggacg
     3901 aaatggttca agtgtattct caaaaaaaac aaagccgatc cgaaaccccg gccgaaatcg
     3961 gcgcacactt ccacccgctg ctcgaccgcc tgtgcgaaac cgcagaagca caaaacgcgt
     4021 ccgacatcct tatcagcaaa ggattcccgc cctcgttgaa aatcaacagc gcattaaccc
     4081 cgcagccgca aaaggcgctg acgggcgagg aaaccgccgc catcgccgca tcgacgatga
     4141 acgccgaaca atcggaaata ttccggcgcg acggcgaaat caactactcc gtccagtcgc
     4201 gcagcggcac gcgctaccgc gccaacgcct accacagcca aggcagcgca ggtttggttt
     4261 tgcggcgcat caaccacgtc atcccgcaaa tgcaggaatt gggcctgccc gaaaaactca
     4321 aagacctcgc cgtcgcaccg cgcgggctgc tgattatcgt cgggcctacc ggttcgggca
     4381 aatccaccac gatggcgact atgctcgaac accgcaacaa aaccctgccc agccatatcg
     4441 ttaccatcga agacccgatt gaatttatct acaaaccgcg ccgctgcatc tttacccagc
     4501 gcgaaatcgg cgtcgacacc ataaactggc agacggcggt acaaaacgct atgcgccaat
     4561 cccccgacgt ggtctgcatc ggcgaagtcc gcagcaggga aagtatggaa tacgcgatgc
     4621 agctcgccca aaccggccac ctgtgcattt ttacgctcca cgccaacacc gcgccgcagt
     4681 cgctcgaacg catactcaac ttctacccca aagaacagca caaccaaata ctgatcgaca
     4741 tcgccctcaa cctgaccggc atcatctgcc aacgcctcgc cctcaaacaa gacaaaacgg
     4801 gcaggacggc ggttgtcgac ttgctcatca acacgcccgc catccaagac ttcatcctga
     4861 agggcgacct gatgaacatc agtaaaatca tggaaaccgc caaaaccgac ggaatgcaga
     4921 cgatggatca aaaccttttc gaactgtacc gtcacggcat catcagttac gaagaagccc
     4981 tgcgccagtc cgtttccgcc aacaacctgc gattgcacat ccaactgcac aaagaaggca
     5041 aaacgcccga actcctttac gacagggtca acggtctcaa cctcatttcc tgatccgcaa
     5101 aacccaatgc cgtctgaaaa ccgcatcccc gttttcagac ggcatgattt tatccgcccc
     5161 attcatgtgc tacactttat agtggattaa atttatagtg gattaacaaa aatcaggaca
     5221 aggcgacgaa gccgcagaca gcacaaatag tacggaaccg attcacttgg tgcttcagca
     5281 ccttagagaa tcgttctctt tcagctaagg cgaggcaacg ccgtactggt ttttgttaat
     5341 ccactatatt cacttcataa caataaaccg gtaaaaccat gaaaacccca ctcctcaagc
     5401 ctctgctcat tacctcgctt cccgttttcg ccagtgtttt taccgccgcc tccatcgtct
     5461 ggcagctagg cgaacccaag ctcgccatgc ccttcgtact cggcatcatc gccggcggcc
     5521 ttgtcgattt ggacaaccgc ctgaccggac ggctgaaaaa catcatcgcc accgtcgccc
     5581 tgtttaccct ctcctcgctc acggcacaaa gcaccctcgg cacagggctg cccttcatcc
     5641 tcgccatgac cctgatgacc ttcggcttta ccattttagg cgcggtcggg ctgaaatacc
     5701 gcaccttcgc cttcggcgca ctcgccgtcg ccacctacac cacacttacc tacacccccg
     5761 aaacctactg gctgaccaac cccttcatga ttttatgcgg caccgtactg tacagcaccg
     5821 ccatcatcct tttccaaatc atcctgcccc accgccccgt tcaagaaaac gtcgccaacg
     5881 cctacgaagc gctcggcagc tacctcgaag ctaaagccga ctttttcgat cccgacgaag
     5941 ccgaatggat aggcaaccgc cacatcgacc tcgccatgag caacaccggc gtcatcaccg
     6001 ccttcaacca atgccgttcc gccctgtttt accgccttcg cggcaaacac cgccacccgc
     6061 gcaccgccaa aatgctgcgt tactactttg ccgcccaaga catacacgaa cgcatcagct
     6121 ccgcccacgt cgattatcag gaaatgtccg aaaaattcaa aaacaccgac atcatcttcc
     6181 gcatccaccg cctgctcgaa atgcagggac aagcctgccg caacaccgcc caagccctgc
     6241 gcgcaagcaa agactacgtt tacagcaaac gcctcggccg cgccatcgaa ggctgccgcc
     6301 aatcgctgcg tctcctttcc gacggcaacg acagccccga catccgccac ctgcgccgtc
     6361 ttctcgacaa cctcggcagc gtcgaccaac aattccgcca actccagcac aacggcctgc
     6421 aggcagaaaa cgaccgcatg ggcgacaccc gcatcgccgc cctcgaaacc ggcagcctca
     6481 aaaacacctg gcaggcaatc cgtccgcagc taaacctcga atcaggcgta ttccgccatg
     6541 ccgtccgcct gtccctcgtc gttgccgccg cctgcaccat cgtcgaagcc ctcaacctca
     6601 acctcggcta ctggatactg ctgaccgccc ttttcgtctg ccagcctaac tacaccgcca
     6661 ccaaaagccg cgtccgccag cgcattgccg gtaccgtact cggcgtaatc gtcggctcgc
     6721 tcgtccccta cttcaccccg tctgtcgaaa ccaaactctg gattgtcatc gccagtacca
     6781 ccctcttttt catgacccgc acctacaaat acagtttctc caccttcttc attaccattc
     6841 aagccctgac cagcctctcc ctcgcaggtt tggacgtata cgccgccatg cccgtacgca
     6901 tcatcgacac cattatcggc gcatcccttg cctgggcggc ggtcagctac ctgtggcccg
     6961 actggaaata cctcacgctc gaacgcactg ccgcccttgc cgtatgcagc aacggcgcct
     7021 atctcgaaaa aatcgccgaa cgcctcaaaa gcggcgaaac ggccgacgac gtcgaatacc
     7081 gcgccacccg ccgccgcgcc cacgaacaca ccgccgccct cagcagcacc ctttccgaca
     7141 tgagcagcga acccgcaaaa ttcgccgaca gcctgcaacc cggctttacc ctgctcaaaa
     7201 ccggctacgc cctgaccggc tacatctccg ccctcggcgc ataccgcagc gaaatgcacg
     7261 aagaatgcag ccccgacttt accgcacagt tccacctcgc cgccgaacac accgcccaca
     7321 tcttccaaca cctgcccgaa accgaacccg acgactttca gacagcactg gatacactgc
     7381 gcggcgaact cgacaccctc cgcacccaca gcagcggaac acaaagccac atcctcctcc
     7441 aacagctcca actcatcgct cggcagctcg aaccctacta ccgcgcctac cgacaaattc
     7501 cgcacaggca gccccaaaac gcagcctgaa aaagtttcgg cattttgtaa gagagggcag
     7561 attgtcagac aggttgcaag atagtggata aagctttgcg cagggtaaat gcgtagcaac
     7621 tgaaccgtca ttcccacgaa aacagaaaac caaaaacaga aacctaaaat cccgtcattt
     7681 ccacga
//
LOCUS       DAXHTR010000106         7621 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.106, whole genome shotgun sequence.
ACCESSION   DAXHTR010000106 DAXHTR010000000
VERSION     DAXHTR010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.106"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(274..2310)
                     /locus_tag="ACFNML_001557"
     CDS             complement(274..2310)
                     /locus_tag="ACFNML_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HFC6392910.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVERLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELSDDGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFADGTLQLPTAYLVCNFAPPVG
                     DKEARLSHDEILILFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YDVLAQMSAHEETGVPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKKVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRGAAESFKAFRGREPRIDALLRHSGFDNAA"
     gene            complement(2398..3270)
                     /locus_tag="ACFNML_001558"
     CDS             complement(2398..3270)
                     /locus_tag="ACFNML_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HFC6392911.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAVVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            3540..5930
                     /gene="gyrB"
                     /locus_tag="ACFNML_001559"
     CDS             3540..5930
                     /gene="gyrB"
                     /locus_tag="ACFNML_001559"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004283954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFC6392912.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASAETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINSYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            6027..7412
                     /locus_tag="ACFNML_001560"
     CDS             6027..7412
                     /locus_tag="ACFNML_001560"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFC6392913.1"
                     /translation="MISIFDIFKIGIGPSSSHTVGPMKAAAAFAAGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNSLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRVAGVADVMEGCIK
                     RGLAADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTALTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKSTYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
BASE COUNT         1721 a         2120 c         2167 g         1613 t
ORIGIN      
        1 ccgtctgaag cgttgtttga ccgtttcaga cggcattttt atcaaattca aaaatattcc
       61 gtcattcccg cgaaagcggg aatctagaac gtaaaatcta aagaaaccgt tttatccgat
      121 aagtttccgc accgacagac ctggattccc gcctgcgcgg gaatgacggg attttggctt
      181 actgtttttg tgaaaatggc gaaaccgtgc aaatgctgat gccgtctgaa acactgtttg
      241 attgtttcag acggcatttt ttgttcacat cgatcaagcc gcgttgtcga aaccgctgtg
      301 gcgcaagagt gcgtctatgc gcggttcgcg tccgcggaag gctttgaagg attctgccgc
      361 gccgcgcgat ccgccgacgg cgaggatttc ctgccaaaag cgtttgcctg tggcggcgac
      421 atcgtcgctt tcttcaaagg cggcgtatgc gtccgcactc aatacttccg cccacgcgta
      481 gctgtaatag cctgcggaat agccgcctgc gaagatgtgg ccgaagctca aggcgaagcg
      541 gttgtattcg ggcggttgga tgacggcgac ttttttgcgc acgctgtcta aaacctgttg
      601 ccagtttttc agacggcctt cgtcgtcttc gctgtaaatc atcatatcga agagggcaaa
      661 ctccatttgg cggacgagga acataccgcg ttggaagttt ttggcggcga gcattttgtc
      721 gaagagttct ttcggcaggg gaacgccggt ttcttcgtgg gctgacattt gtgccaagac
      781 gtcgtattcc cagacgaaat tttccataaa ctggctgggc agttcgaccg cgtcccattc
      841 tacgccgttg atgccggata cgcccagttc gtccacttgg gtaagcaggt ggtgcagccc
      901 gtgtccggtt tcgtggaaga ggatgaggat ttcgtcgtgg ctcaagcggg cttctttgtc
      961 gccgacgggc ggggcgaagt tgcagacgag gtaggcggtg ggcagttgca gcgtgccgtc
     1021 ggcgaagcgg cggcggcctt tgtagtcgtt catccacgcg ccgccgcgtt tgccttcgcg
     1081 tgcgtacaaa tccatataaa cgccgcctat ggtttcgccg ttttgttgca attcaaaata
     1141 gcgcacgtct ttgtgccaga cggggacggt tttttcggta aatccgatgc cgtagagttt
     1201 tttgatttgg gcgaacagtc cgtttaatac tttgccgacg gggaagtatt ttttgacttc
     1261 ggtttcgctg aacgcgtatt tggcttcgcg cagtttttcg ctggcgtagc ccaagtccca
     1321 cggttgcaaa tcggcgaggt tcaggctttc gcgggcgaag gctttgactt cggcgaggtc
     1381 tttttcggcg tagggtttgg cgcggcgggc gaggtcgtgc aggaagttta aaacttgttc
     1441 gggcgtgtcc gccattttgg ttgccagcga caattcggcg tagtttttga agccgagcag
     1501 tttggcggtt tgcagggcgt ttgcgagcgt gcggtcgatg ttggcggtgt tgtcgaattt
     1561 gccgtcgtct gaaagttcgc tggcgcgggt aacgtaggcg cggtagattt gttcgcgcag
     1621 ttcgcggttg tcggcgtatt ggatgacggc gaggtagtgc ggaatctgca aaccgatttt
     1681 gtagcctgtt ttgccttcgc tttgcgcggc ggcggcaaac atggcgagcg cgtcttcggg
     1741 aatgccggca agcggtgcgg catcgtcaaa gtaaatgccg aacgcgtcgg tcgcgtctag
     1801 gacgttttgg gagaatttgg cggaaagttg cgcgccttcg gtttgcagtt ttgccagttc
     1861 tgcctgctgt tcgggcggca gttccgcgcc gctgaggacg aaatcgcgca gatcgtggtt
     1921 gagtttggtt ttttgcgcgg gggagagggt gtcgaactcg ggggagtttt tgatggtttt
     1981 gaagcggttg tacagctcga tgtcttgtcc gatttcggtg aagaagacgg tgatttcggg
     2041 catcagttcg ttatagacgg cgcgcagttc gggcgtgtcg gcgacggagt tgaggtgcga
     2101 caccacgccc caaatcctgc cgacgcgctc ggtgatgccg gtcagacgct cgacggtgtt
     2161 cgcccagccg gtgtgcgttt gggctttgat ggcggcgatt tgttcgcgcg cttcggcaat
     2221 ggcggtttgc agggcgggtt tgatgtcttc ggttttgatt tgatcaaaac ggggttcttc
     2281 gcccaaatgg agcagtgcgt tgtcagtcat aagatgggtt tcctttgcgt gttgtgcttc
     2341 agacagcata tcggcaatat gccgtctgaa gccggaaatg gggtcggtcg gggaaagtta
     2401 aagtgtttcg gagccggcgg ggtaatttaa caggcggtat tgtacgcgca aaaattcgtc
     2461 cataaccgcc tgttggattt ccagccgctt tgaaacgggg gaggcgaagc ggacgatgat
     2521 gcggtatgcc ttgtcatcgt acggcacgcg ggtaacgcgc ggtctggcgg cgggcgtgat
     2581 aaacagtttt tccgcctgca cgttttccaa atgccgttgg atggcgggga tgtagggcgc
     2641 gcacaagggc tcgagtacgg ctttcagacg gcatacggct tcatccgaat ccaaatggat
     2701 ggggacgggg atttcgaccg tatggatgac gtagtcgccc aaaatattgt cgcggcgcac
     2761 gggatggctc aacaacaggc tgttggggaa agaaacggtg gttcccgcaa gctgtccgac
     2821 cagggggttc ggaccgacct gcatcatcag cgtgttcaac aggttgatgt cgaccacgcg
     2881 cccgcgcagg ccgttgattt cgatatagtc gccgaccgag tattgctggg tggcagacct
     2941 taaaatactg cccgacagac acataatcag ttcctttgtc gccacgacga ccgccgccgc
     3001 caccgcaaac atcgacaaag ccagcgtttg gatttgcgcc gaccagataa atgccagcga
     3061 aaacagcacc aaaagcagcg ttatattgcg gctggcaacc aaaaaccgcc gcttgctttc
     3121 gatgccgaaa tccggatgcc gtttgaagtg gatattcaac agaagggcgc gcgccagcag
     3181 caaagccgcg accaccgcca cggattcgac cgcctccgca cgtatcggga cggcaccgag
     3241 ccaagtgtcc aacatattcc atatttccat ttccgcgccc ctgtccgaaa agtatagggt
     3301 gtagatttta gtggcaaaaa aacgcttttg ccactttacg ggacaatccc cgacctgatg
     3361 ccggaggcaa acgtaccgtt tcatcttttc ccggcggcac actgatgcgg ataacgctga
     3421 attaggcggc aaaatcggct ataatacaca attatatatg ccgtcccgcc cgctttttct
     3481 acaaggtgcg gacttgtttt ggcggcatcg caaaatcttt tcaaaatccg gcaaaaaata
     3541 tgactgaaca aaaacacgaa gaatacggcg ccgacagcat tcaggtgctc gaaggcttgg
     3601 aagcggtacg caaacgcccc ggtatgtaca tcggcgacac gcaggacggc agcggcctgc
     3661 accacatggt gttcgaagta ttggacaacg ccattgacga agcactcgcc ggacattgcg
     3721 acaaaatcac cgttaccatc cacgccgacc attccgtcag cgtcgccgac aacgggcgcg
     3781 gtatgcccac cggcatccac ccgaaagaag gacgctccgc cgccgaagtc atcatgaccg
     3841 tattgcacgc gggcggtaaa ttcgacaaca acagctacaa aatctccggc ggcctgcacg
     3901 gcgtgggcgt gtccgtcgtc aacgcgctgt ccgactgggt aacgctgacc atctaccgcg
     3961 acggcaaaga acacttcgtc cgcttcgtgc gcggcgaaac cgaagagccg ttgaaggttg
     4021 tcggcgattc cgataaaaaa ggcacgaccg tgcgcttcct cgccagtgcg gaaaccttcg
     4081 gcaacgtcga atacagcttc gacatcctcg ccaaacgcat ccgcgaactt tcgttcctga
     4141 acaacggcgt ggacatcgaa ttgaccgacg agcgcgacgg caaacacgaa agcttcgccc
     4201 tttccggcgg cgtggctgga ttcgtgcaat acatgaaccg caaaaaaacg cccttgcacg
     4261 aaaaaatctt ctacgcgttc ggcgagaaag acggcatgag cgtcgaatgc gcaatgcaat
     4321 ggaacgacag ctatcaggaa agcgtgcagt gcttcaccaa caacatccct cagcgcgacg
     4381 gcggtacgca cctgaccgcg ttgcgccaag tgatgacgcg caccatcaac agctacatcg
     4441 aagccaacga agtcgccaaa aaagccaaag tggaaaccgc cggcgacgat atgcgcgaag
     4501 gtttgacctg cgtgttgtcc gtcaaactgc ccgaccccaa attttcgtcc caaaccaaag
     4561 acaaactggt ttccggcgaa atcggtcccg tcgtcaacga agtcatcagc caagccctga
     4621 ccgacttcct cgaagaaaac cctgccgaag ccaaaatcat caccggcaaa atcgtcgatg
     4681 ccgcccgcgc gcgcgaagcc gcccgcaaag cccgcgaaat cacccgccgc aaaggcgtaa
     4741 tggacggctt ggggctaccc ggtaaactcg ccgactgcca agaaaaagac cccgcattgt
     4801 cagaacttta tctggtcgag ggcgattctg caggcggttc cgccatgcag gggcgcgacc
     4861 gcaaattcca agcgattttg ccgcttaagg gcaagatttt gaacgtcgaa aaagcgcgct
     4921 ttgaaaaaat gctcgccagt caggaagtag ccgctttgat taccgcgttg ggcgcgggca
     4981 tcggcaaaga agaattcaat gccgagaagc tgcgttacca ccgcatcatt atcatgaccg
     5041 atgccgatgt ggacggcgcg catatccgca ccttgctttt gaccttcttc taccgccaaa
     5101 tgcccgaact ggtcgagcgc ggctacatct atatcgccca gccgcctttg tataaagcga
     5161 aatacggcaa acaggaacgt tacctcaagg acgagttgga aaaagaccaa tggctgctcg
     5221 gtcttgcctt ggaaaaagcc aaaatcattt cagacggccg caccatcgaa ggcgcagaac
     5281 ttgccgacac cgccaaacaa ttcctgttgg caaaaaccgt catcgaacag gaaagccgct
     5341 tcgtagacga actcgtcctg cgcgccatgc tacacgcgtc gcccattgat ttgacgtcgt
     5401 ctgaaaacgc cgataaagcc gttgccgaac tttccggtct gcttgacgaa aaagaagtcg
     5461 ccctcgaacg catcgaaggt catgaaggac accggttcat caaaatcacg cgcaagctgc
     5521 acggcaacgt catggtcagc tacatcgaac ccaagttcct caacagcaaa gcctaccaaa
     5581 ccctcaccca aaccgccgcc gcgctcaaag gcatggtcgg cgagggtgcc aagctttaca
     5641 aaggcgaaaa cgggtacgac gcggacagct ttgaaaccgc tttggacatc ttgatgagcg
     5701 ttgcccaaaa aggtatgtcc atccaacgat acaaaggctt gggcgagatg aaccccgagc
     5761 agctgtggga aaccacgatg gatcccgccg tgcgccgcct gttgaaagtg cgcatcgaag
     5821 atgccattgc cgccgacgaa gtgttcgtta cgctgatggg cgacgaggtc gagccgcgcc
     5881 gtgcctttat cgaaaacaac gcgctgattg cccaaaatat cgacgcataa gtgccgtttt
     5941 aaaaaaggag acgggcatcg tgccgcgtct cctttttggt tggtcaaacg gaacctgtgc
     6001 cgtctgaaaa accgtcggag caaaatatga tcagcatttt cgatattttc aaaatcggta
     6061 tcgggccttc cagttcgcat acggtcggcc cgatgaaggc agccgccgcc tttgcggcag
     6121 gtttggatgc acaggctgtt cgcatcgtca tcgacattta cggctcgctc gcactgaccg
     6181 gatacggaca cggtacattt gacgcgctga tgctcggttt ggaaggcagc ctgccgcacg
     6241 acatcccgct tgccggcatt cccgaacgcc tcgaacgcat ccgcacgcag cacatcctcc
     6301 ggctcaacgg gcaagaaatc cgcttcatcc ccgaccgcga cctgaacata ctcggcaatc
     6361 aagtgctgcc caaacacccc aacagcctgc gttttaccgc ctatgcttca gacggcacgg
     6421 tattgaatga acaggtttat tattcggtcg gcggcggctt tgtcgttacc gaagaagatt
     6481 ttgaccggca ggcggaaacg gaaaaagccg ttccctatcc ctataccagt tgcgccgaac
     6541 tgcttgcccg atgccgtctg aaccggctcg acatctccga agtcgtgttg gcaaacgaag
     6601 ccgcgcttgc cggatgcggc gaagccgaaa tccgccgccg cgttgccggc gttgccgatg
     6661 tgatggaagg ctgcatcaaa cgcggtttgg cggcagacgg cgagctgccc ggcggattga
     6721 acgtccgccg ccgcgccccg cagcttgccg ccaagctcaa agtcctgcgc gaaaccgaaa
     6781 tcgtcaacac ccagctctgg ccgatggtgt acgccatggc ggtcaacgaa gaaaacgccg
     6841 ccggcggacg cgtcgttacc gcactgacca acggcgcggc aggcatcatt cccgccgtat
     6901 tgcactattt ccgcaagttc aatccgcacg ccacacagga acgcgtcgaa aacttcctgc
     6961 tcaccgcagg cgcaatcggc atcctctaca agaccaacgc ctccatttcc ggtgcggatg
     7021 tcggctgtca gggcgaagtc ggcgtagcgt gttcgatggc ggcgggcgca tacgccgaag
     7081 tcatcggcgg cacgcccaaa caagtggaaa acgccgccga aatggcgatg gaacaccatt
     7141 tggggctgac ttgcgacccc gtcggcggac tggtgcaaat cccctgcatc gagcgcaacg
     7201 gcatcgccgc cgaaaaagcc ctcaaactcg gcacgctcgc gcttttggaa gacggcacgg
     7261 acaaaaaagt ctcgctcgac gaagtcatcc gaaccatgtt acagacaggg cgcgacatga
     7321 agtccaccta caaagaaacc tcgcttgccg gactcgccgc cacgctccgg aaaaaagccg
     7381 tccccgtatc cgtgcgcgtg gtcgagtgct gaggatggac agaaacgaaa atgccgtctg
     7441 aaaacagttt tttagacggc atttcattta tttcttgcgg ctttaccgtt tcagtgccag
     7501 cgtcaacacg cccgccgtaa ccagccccaa gcctatccat tcctgcgtgt tcgggcgttc
     7561 gtctaagaaa accaccgcca tcaggaatgc aaaccggcgt tgtttgcagc atccggcaag
     7621 a
//
LOCUS       DAXHTR010000107         7415 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.107, whole genome shotgun sequence.
ACCESSION   DAXHTR010000107 DAXHTR010000000
VERSION     DAXHTR010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.107"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(232..987)
                     /locus_tag="ACFNML_001561"
     CDS             complement(232..987)
                     /locus_tag="ACFNML_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HFC6392914.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGE
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(1167..2339)
                     /gene="nirK"
                     /locus_tag="ACFNML_001562"
     CDS             complement(1167..2339)
                     /gene="nirK"
                     /locus_tag="ACFNML_001562"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HFC6392915.1"
                     /translation="MKRQALAAMIASLFALAACGGEPAAQAPAETPAASAEAASSAAQ
                     TAAETPSGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWT
                     FDGDVPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTST
                     FSFKALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKG
                     KKGAQGLQPFDMDKAVAEQPEYVVFNGHVGSIAGDNALKAKAGETVRMYVGNGGPNLV
                     SSFHVIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRA
                     FNKGALGQLKVEGAENPEIMTQKLSDTAYAGNGAASAASAPAASAPAASASEKSVY"
     gene            2711..4966
                     /locus_tag="ACFNML_001563"
     CDS             2711..4966
                     /locus_tag="ACFNML_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HFC6392916.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRVKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPKLQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPGEVFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEIPAEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IVISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLIFIGGAICVAI
                     QATKIVFGRDK"
     gene            5145..5678
                     /locus_tag="ACFNML_001564"
     CDS             5145..5678
                     /locus_tag="ACFNML_001564"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HFC6392917.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(5741..6925)
                     /locus_tag="ACFNML_001565"
     CDS             complement(5741..6925)
                     /locus_tag="ACFNML_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HFC6392918.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDSQRAADFQDVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDVGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDPSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLNASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(6965..7369)
                     /locus_tag="ACFNML_001566"
     CDS             complement(6965..7369)
                     /locus_tag="ACFNML_001566"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HFC6392919.1"
                     /translation="MTAQPCPICTAQDEDILLQTPNLRVIAIHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFQPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1669 a         1791 c         2088 g         1867 t
ORIGIN      
        1 aaagttgcgg gaatgacgaa aagttgcggg aatgacgaaa agttgcggga atgacggaaa
       61 gttgcgggaa tgacggaaag tggcgggaat gacggttcgg gcattcctta aattacccgt
      121 gtatcgctgt aaatcttaga gatggcggaa tatagtggat taacaaaaac cagtacggca
      181 aggcgaggca acgccgtact ggtttttgtt aatccactat aattgaaggg gttatcggct
      241 tgtgcaacgg aagcccaagt tgtgcaagac atatttggat tgcaggctgg tacggatgcc
      301 gtagcggagg aaggcggcat agttggacga gtcgctcgac ccgatagacg cgccgctgca
      361 aaacatttgc gcgttggcat tgccggaaga aagcaggctg ctgttgaaat cttccgtcca
      421 ttcccaaatc agcccgtgca tatcataaac gccccagtag ttcgggcggc ctttgccgac
      481 atcgtgcagg cctttccgtc cgccgtcggc ataccaatcg agaatagtgc ggttgtagcc
      541 gggttcgttt gagccgtttt tctgcgtggc ggaagcaagt ccggcaaatt cccattcgtc
      601 aatggtcggc aggcgtttgc cttgtgcggc gcaataggcg ttggcggcaa accaggaaac
      661 attggttacc ggttgtttta attcgcccgc cttcggcgca tagctgcggc tgccgttttt
      721 catccaatgc ttcaggtaag cgggttctgc ctgtttggaa ccgatcctgc ctttttgcca
      781 ttgggggtgg ctgttgacaa attcggcaaa ctcggcattg gtaacgggat atttatccag
      841 tttgaacggt ttgactttaa tcaggccggt atcttttttc agataaagcg ggcggtagct
      901 gccgccttcg atttgaacca tttcggcagc cgccgcttga gtgccggcga gtgccgcaca
      961 gagaaagagt aaccgggcaa acttcatatc atgcctcctg acaggcagtt aaaattgatt
     1021 tttcagacgg cattttaaac aaaaagccgc gaatgccgtc tgaagaatca atcttccgaa
     1081 aggaaagatt ggttgttaaa aaaccactgc cgtgcgtaat taagtacggc ggcagtggtt
     1141 tgttccgcta atgacgggta tccgatttaa taaacgcttt tttcggatgc agaggctgcc
     1201 ggggcggaag ctgcgggagc ggaagcggca gaagccgcac cattgccggc gtaagcggta
     1261 tcactcaatt tttgagtcat aatttcaggg ttttccgcac cctctacttt caattggccc
     1321 aacgcgcctt tgttgaatgc gcggaagatg gagtggtcga ccaaagtgta gctgcccggg
     1381 atgtcgactt tgaattcgac aatggcagag ccgccggcag gaacgatggt gctttgtacg
     1441 ttttcgttaa tcagcttgcc gccttcaaca taaactttgt cgaagatttc gccgatgacg
     1501 tggaaggagg ataccaggtt cgggccgccg ttaccgacgt acatacgcac ggtttcgcct
     1561 gctttggctt tcagcgcgtt gtcgccggcg atagaaccta cgtggccgtt gaatacgacg
     1621 tattcaggct gttcggcgac ggctttgtcc atatcgaacg gttgcaggcc ttgcgcgcct
     1681 tttttgcctt tggtgtagaa gtcgccttgg acgatgtaga actctttatc cactttcggc
     1741 agaccttctt taggctcgac caaaatcaga ccgtacatac cgttggcgat gtgcataccg
     1801 accggcgcga cggcgcagtg gtagatatac aggcccggtt gcagggcttt gaagctgaat
     1861 gtggaagtac ggcccggggc ggtaaaggtt gcagccgcgc cgccgccctg gccggtagcc
     1921 gcgtggaagt cgacgttatg gggaacggta gaagaaggat tgttggaaaa ttccacttca
     1981 accgtatcac cttcgcgtac gcgaatcata cgacccggaa cgtcgccgtc aaatgtccag
     2041 tagcggtatt ccacaccgtc gtccattttc atggtttttt cgacggtttc catttttacg
     2101 cggactttgg cagggtagtc gcggtcgatt gcaggaggca cttcgggagc gtgcgtggta
     2161 accgcatcga taacgggcag ttcgccggac ggcgtttcgg cagcagtttg tgcggcggaa
     2221 cttgcggctt cggcggaagc ggcaggggtt tcggcagggg cttgcgcggc aggttcgccg
     2281 ccgcaggcgg ctaaggcgaa taaggaagca atcattgcgg ctaatgcttg gcgtttcata
     2341 atgttttcct tttgtaagaa aagtagggag attggttaag aactgacgtg attatgcggt
     2401 tgcttactta tgtatatttt gatttaggtc aaataattca ataaatatga acttaaaatg
     2461 acacttctaa tactttataa ctactaaaaa ttacaataaa taagctgatt taccgggtgc
     2521 ttcggcaaat caggcaaagg cagggcagtt tttgagtaaa gaataatgat agttattatc
     2581 atatatgtat ttgttttata tgtgagtttg tttgaacaaa tatttatttg cacgacaaac
     2641 cgtgctacaa tctttaacat tcatattctg tgaattttaa acaactgctt tattaaaagg
     2701 agctctcaaa atgggacagt acaagaagct gtggtacttg ctgttcgccg ttctggcggt
     2761 atgctttacc attctcggct atatgggcag cgaggtttat aagaaagccc cgccttatcc
     2821 cgaacaggtc gtttccgcat caggcaaagt gctgatgacg aaagacgata ttttggcagg
     2881 tcagtctgca tggcagagta ccggcggtat ggaagtcggt tcgattttgg gtcacggcgc
     2941 atatcaggct ccggactgga cggccgactg gctgcatcgt gagttgtctg cctggttgga
     3001 tttgaccgcg caacagactt acggcaaaaa atttgatgaa gtttcccctg aagaacaagc
     3061 cgttctgaaa acccgcctgg ctgacgaata ccgcaaccaa agccgtgtga aagaagacgg
     3121 cagcgtcgtc atcagcgata cgcgcgtgaa agccatcgaa agcatcctgc cttactatca
     3181 cggcgtttac ggcgacgatc ccaagctgca aaccacccgc gaacactttg caatgaaaaa
     3241 caacacattg ccaagccaag aagcgcgtga aaaactgttc gacttcttct tttggacttc
     3301 ttggtctgct tcgaccaacc gtcccggcga ggtctttacc tacaccaaca actggccgca
     3361 cgagcctttg atcaacaatg tgccgaccac tgaaaactat atgtggtcgt ttaccagcgt
     3421 tgttcttctg ctgatgggca tcggtttgct gatgtggggt tattccttcc tgaccaaaca
     3481 cgaggaagtg gaaatcccgg ctgaagatcc gatttccaaa atccaactga ccccttcgca
     3541 aaaagcattg ggcaaatacg tcttcctgac ggtcgccctg ttcgtggtac aagtattgtt
     3601 gggcggcctg accgcgcact ataccgtcga aggtcagggt ttctacggca tcgatgaggc
     3661 gttgggcttt gaaatgtccg actggttccc ctatgccctg acccgtactt ggcacatcca
     3721 atccgccatc ttctggattg caaccggctt tttgacggca ggtttgttcc tcgcgccgat
     3781 tgtcaacggc ggcaaagatc ccaagttcca acgtgcaggc gtgaacttcc tctatatcgc
     3841 cctgtttatc gtggtcggcg gttcttacgc gggcaacttc ttcgcgttga cgcacatcct
     3901 tccccccgaa tttaacttct ggttcggaca ccaaggttac gaatacctcg atttgggacg
     3961 tttctggcaa ctcctgttga tggtcggact gctgttgtgg ctgttcctga tgttgcgctg
     4021 cacggtttcc gccttcaaag aaaaaggcgt ggacaaaaac ctgctggcaa tctttgtcgc
     4081 ttctatggtc ggtgtgggcg tgttctacgc accgggtctg ttctatggcg aaaaatcccc
     4141 gattgctgta atggaatact ggcgttggtg ggtggttcac ctgtgggtgg aaggcttctt
     4201 cgaagtattt gccactgccg cctttgcctt tgtcttctac aatatgggct ttgtccgccg
     4261 cagtaccgcc acggcctcca ctttggccgc tgccgccatc tttatgttgg gcggcgttcc
     4321 gggtacgctg caccacctgt acttctccgg ctctacctcc gcctctatgg caatcggcgc
     4381 ctgcttctct gctttggaag tcgtgccgct ggtgctgctg ggccgtgaag catacgagca
     4441 ttggtcttac caacacctgt ccgactgggc gaaacgcctg cgttggccgc tgatgtgctt
     4501 cgtggcagtc gccttttgga atatgatcgg tgccggcgta ttcggcttcc tgatcaatcc
     4561 gccgatttcc ctgttctata tccaaggttt gaacacttct gccgttcacg cgcacgccgc
     4621 cttgttcggt gtgtacggtt tcttggcatt gggcttcgta ttgctggttg cccgttattt
     4681 gaaaccgaac gcgcagtttg acgacaaact gatgacttgg ggcttctggt tgctcaacgg
     4741 cggcttggtc ggtatgattg ttatcagcct gctgcctgtc ggcgcgattc aggcatacgc
     4801 ctccatcacg cacggtctgt ggtatgcccg cagcgaggaa ttcctgcaaa tggaaatcct
     4861 cgacaccctg cgctgggttc gtactgccgc cgacttgatc tttatcggcg gtgcaatctg
     4921 tgtcgccatc caagccacta aaatcgtatt cggccgggac aaataagttt tgcggtatga
     4981 aatgagcaac tgcacccgtt ttcgggtgca gttttttatt gcggattggg acgggaatgg
     5041 aaaaggatgt ggatttttta ttttaatcgg ctatacaatt gctgctttca tgccgtctga
     5101 agcacgtttc agatggcatc ggtttacatc agataaggat aaaaatgggt atttacgatt
     5161 ttcaaatgaa agatgcggaa ggcaatgcgg ttgatttgtc aggctatcgc ggcaaggttc
     5221 tgctgattgt caacacggca acgcgttgcg gtctgacccc gcaatacgag gctttgcaga
     5281 agctgtatgc acaatatacc gcagaaggct tggagatttt ggattttccg tgcaaccagt
     5341 tccgcgaaca ggctcccgaa agcagcggag aaattgccca agtgtgtatg atgaagtttg
     5401 gcacgaagtt taagattttc gacaaaatcg aagtcaatgg agcaaatacc gcgcccttgt
     5461 atgcctacct gaaatccgtc aaaccgcaag ataaaggcaa tcatctgttt aaagatttcg
     5521 tgttgaagct cgcagctttg ggagagaagc gcgacgaagg cgacatcaaa tggaacttta
     5581 ccaaattcct cgtcaaccgc gacggcgaag tggtcgagcg ttttgcgcct agtgttacgc
     5641 ccgaagaaat cgaagccgat attcgggcat tgctgtaatc tgccgccgtc ttgggaaatg
     5701 ccgtctgaag gcttcagacg gcatttgtcc gtttgcgggt ttattccgaa atatcggcaa
     5761 caccgctttc ggggaagatg ggcaaatccg gcggtgggag cagggtatcc aaagtcaggg
     5821 cggacgcatt gagtgtcggg tagccttcaa acgcgaccga ataaccgctg tttccgttca
     5881 tacggattcg ggcgtgcgcg cctagaggga aagtgatttt gtcggcaatg tgtccgaacg
     5941 ggaagcccgt cagcacgggg attttcgccg tgcgcgaaac atggttggca acggcggaaa
     6001 aatcataaga cggatcatag acatctcgaa ttttttccat acggaaattg ccgaacacga
     6061 tggcgcgctg tttcttcaaa atacccgaaa gatacagcgt attgagcata cgttcgatgc
     6121 ggtagggctg ttcgccgaca tcttcgagga acaaaatgcc gccgtcgatg tcgggcatat
     6181 aaggcgtacc ggcgagcgag gcgaggacgc ttaagttgcc gccccacaac gtgccttcgg
     6241 tttcgacatc ggcgcgttgg atataaggaa catcaacggt caggcggttt tgggttgcac
     6301 ccttgataaa ggcatccatc gtaaacgcac cgggggcggg tttgccaaaa tcgctataag
     6361 ccatcgggcc ggcaaaactc atcatattgc cttttgccaa caatgccagc tggacggcgc
     6421 atacgtcgct gaatccgaaa aacagcgtgc cgtgttcgcg catccttgcg ccgagcgaag
     6481 caaaatcgat atgcggcaga atgcgtgccg caccataacc gccgcgcaag cccatcagca
     6541 ctttaggtgt ggcaacgcga ccggaagcga catcttggaa atcggcggca cgttgtgagt
     6601 ccgtgccggc aaaccgttgg aaacggcggc tgcccgcctg ttggttggtt acggtaaaac
     6661 cgacattgta aaggcgggtt aaggctgtgt tgacgcggtt ggtgtcttcg gcaaatcctg
     6721 acgaagcgac aacgcgcaga aggttgccgt ccgaactttg acggcgtggc gtttggagag
     6781 gcacggttcg ggctttcaca acggaatggg aagagggaag gggcggaacg gatgtggcgg
     6841 atgtgccgca agcctgaagc agccccgcgc cggcagcggc ggtgcaggtt ttcagaaaac
     6901 ggcggcggga ggtcggttcg gtcataattt ccctttcata cggtttcaga cggcatcggc
     6961 gggcttaaag cagcttttta agctgttccg tccaatcttg cggcagggtc ataccgtgtt
     7021 tccggacggg gtttgcccaa atcggcgcgg ggaaggacgc atcgttttca aagcgggcga
     7081 taatatgcca gtgcaggtgc ggcacgacat tgcccaagct ggcgaggtta atcttggcag
     7141 gttgaaacac ttggcgcata gcggcttcga ctttgtacac catttccatc aattcgccgc
     7201 gttccgctgc cgaaaggtcg gtcatttcgg cgatgtgctt acgccaaatg acgcggcaga
     7261 atgcaggcga accgctgtcg ttatggatgg cgatgacgcg gaggttgggg gtttgcagca
     7321 aaatatcttc atcttgtgcc gtgcagatgg ggcagggttg ggcggtcatt tttccttatt
     7381 cctgaaagtg tttaaaaaac ggtaggataa tttct
//
LOCUS       DAXHTR010000108         7243 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.108, whole genome shotgun sequence.
ACCESSION   DAXHTR010000108 DAXHTR010000000
VERSION     DAXHTR010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7243
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.108"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(93..452)
                     /gene="rnpB"
                     /locus_tag="ACFNML_001567"
     ncRNA           complement(93..452)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACFNML_001567"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(531..1634)
                     /gene="prfB"
                     /locus_tag="ACFNML_001568"
     CDS             complement(531..1634)
                     /gene="prfB"
                     /locus_tag="ACFNML_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFC6392920.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVVGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1737..2630)
                     /locus_tag="ACFNML_001569"
     CDS             complement(1737..2630)
                     /locus_tag="ACFNML_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HFC6392921.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIRL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2972..3421
                     /locus_tag="ACFNML_001570"
     CDS             2972..3421
                     /locus_tag="ACFNML_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFC6392922.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3495..3803
                     /locus_tag="ACFNML_001571"
     CDS             3495..3803
                     /locus_tag="ACFNML_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392923.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3919..4761
                     /locus_tag="ACFNML_001572"
     CDS             3919..4761
                     /locus_tag="ACFNML_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HFC6392924.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLKNVDADRVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4751..5497
                     /locus_tag="ACFNML_001573"
     CDS             4751..5497
                     /locus_tag="ACFNML_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HFC6392925.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLHEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLST"
     gene            5519..6106
                     /locus_tag="ACFNML_001574"
     CDS             5519..6106
                     /locus_tag="ACFNML_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HFC6392926.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6112..6291
                     /locus_tag="ACFNML_001575"
     CDS             6112..6291
                     /locus_tag="ACFNML_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HFC6392927.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPHCREYKRQL
                     QTIKISLAKTTRTSK"
     gene            6523..6963
                     /locus_tag="ACFNML_001576"
     CDS             6523..6963
                     /locus_tag="ACFNML_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-adhesin E family protein"
                     /protein_id="HFC6392928.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
BASE COUNT         1850 a         1929 c         1636 g         1828 t
ORIGIN      
        1 ggtttttgtt aatccactat aaaaacacaa cctaaataaa aatgccgtct gaaccatatt
       61 tcaggtttca gacggcattt gcgtgtcgga tgcacaccgg acgggcggta agccgggttc
      121 tgtctcggac agtcattcct ctaggcatac cgttaccggt atgctcaagc aacctacccg
      181 aacgctcggc gggcagcgtc attgcgttct gtttggtctt gctccgaatg gggtttggcc
      241 tgccgcatat tgttaccaaa tgcgcggtgc gcccttaccg caccttttca cccttgcctg
      301 tgctgccaaa gcagccatcg gcggttttgc tttctgttcc actttccgtc gcgttaccgc
      361 gcccggccgt taaccggcat tctaccctgc ggagcccgga ctttcctccc cgtatgcctt
      421 acgcgatacg cggcgactgt ctgcccgtcc cgtgtgcggc gcggattata acacgaaacg
      481 caaaaatgcc gtctgaaacg gtacaggttt cagacggcat acagcctaaa ctacacgccc
      541 tgtttcaggc tggcttcgat aaagccgtcc aaatcgccat ccaatacggc tttggtgttg
      601 ccgacttcgt agcctgtacg caagtctttg atacgtgagg aatccaaaac atacgaacgg
      661 atttggctgc cccaacctac atcggattta ccttcttcca acgcctgttt ctcttcattg
      721 cgtttgcgca tttccaattc atacagtttg gacttcaaca tttccatcgc ggcggctttg
      781 ttggcgtgtt gcgaacggtc gttttgacat tgcaccacaa tccccgtcgg ctcgtgggta
      841 atgcgcacgg cggagtcggt tttattgatg tgctgaccgc ccgcacccga tgcgcgatag
      901 gtgtcgatgc gcaaatcggc ggggttgatt tcgatttcga tggaatcgtc gatttcggga
      961 tagacgaaca cggaggcaaa cgaggtatgg cgtttgttgt tcgagtcaaa cggagagtaa
     1021 cgcaccaagc ggtgaacacc tgtttccgtg cgcagcaagc cgtaggcata ttcgccttcc
     1081 actcggatag tcgcgcggtt gatgcccgcg atttcgccgt cgtcttcttc gaggatttcg
     1141 attttgaagc ctttgcgctc ggcgtagcgg ctgtacatgc ggaacagcat acccgcccag
     1201 tcttccgctt ccgtaccgcc tgcgcctgcg gtaatgtcga taaagcagtt gttcggatcg
     1261 gcgggctggt tgaacatccg tttgaactcc aaatccgcca tctgtttttc cagccccgcc
     1321 acatcttcct gcacggcggc aaaaccttct tcgtcgtttt cttcgacggt catttcaatc
     1381 agcatgcggt tgtcttcgat gcccgaagcg atgttgtcga gcgtcaacac gatgccttcg
     1441 aggattttgc gctctttgcc gatttcttgg gcgcgtttcg ggtcgttcca aagttcgggg
     1501 tcttcggaaa gaccgacaac ttcttccaat cggtctttct taccctgata atccatataa
     1561 actcggatgt cttcgctgcg cttttccaaa tcattcaggg tattgttgag ctggttgatt
     1621 acttcggctt ccatgattct tttgttcttt caaaatttta ggggcgtatt gtacgggatt
     1681 cgggtatttt tttctatgga taaagccttc tggaaacacg ttcggacggc atagcgtcaa
     1741 taacggtatg ccgccagttt gcgtttgatt tcaggcaatg cggcacgtgc tgcctcctca
     1801 cccaaccgga tggcgcgttt tttctgatcg aatccgccga ctgcacccaa atccaaaacc
     1861 tgcggtttga taaccacatc cgcctgcccc aactcatttt gcaacgcgga aacgctcatt
     1921 acgttcagcg tctgatcgag ataagagaag aagccttggc tgatgttttt gcccggacgg
     1981 gcggaaatat cgacggcaat cacgaaattc gccccctgcc gccgggcggc actgacgggc
     2041 acgggctgcg acagaccgcc gtcaacatat gtatgcctgc cgatgataac gggttggaac
     2101 acattgggaa tggcggcgga agcgcgcaca gcctgcccgg cattcccttg attgaaagcg
     2161 acggccttgc cggtttcaaa atcagtagca acagcggcaa atttgatggg aaactgctga
     2221 atctgcctgc cgccgacttt tcggttgatg taattttgca gcttttcgcc tttgataaaa
     2281 ccactggtgg acaaggttaa atcgactaaa tcggttttgc ctaaaatttc ggcttccaat
     2341 tcgaggcggt cgggcgacat acccgatgca aaaaggctgc cgactatcga acctgccgat
     2401 gtgccggtaa ccaccttcac aggaataccg ttttctttca aaaccttaat aatacctaca
     2461 tgggcaaatc ctttagatgc gccgccaccg agtgccaaac cgaccactgc ggcgggtttg
     2521 gcggtttgca ccggcttgcg ggcagcatta tttcccgccg tgccgcaggc ggcaagcaac
     2581 gcggcggcgg cgattgccaa aagcggtctg atttttgaaa acgttaccat attttccatt
     2641 cctttatata tcgcaccccg tcaaaaagag ggattgcttt tcttaacaac cccctttgac
     2701 agccaagcaa atgggggctt tgttaagtca tcatcaaaat taatatttct ttttttcctt
     2761 tacggaaatt atatttgaag gcatactatc caaggcggga attatctcac aacaccgccg
     2821 ttatccaaat atcccgcctt tttcctttcc tttccatcaa aatactttct ttttatattc
     2881 attaacttgt taaatcattg gctgccgggt gtcagttttt ccgacaaaat ccgtctaatg
     2941 gggtatcaac agaaccaaaa caggaacact tatgaaaatc ggaacaactt ggcagacggc
     3001 atccgctatg ctggttttgc gtctgtttgc cgcatatgaa tttttggaat cgggtttgca
     3061 aaaatggaac ggggagaatt ggttttccga aatcaacgat cagtttccat tcccgttcaa
     3121 cttgctgccg gacgcgttaa actggaatct cgccatgtat gcggagcttt tactgcccgt
     3181 actgttgctt ttgggtttgg caacgcgtct gtcggcatta gggctgatgg tcgttaccgc
     3241 cgtcgcttgg gctgcggttc acgccggttc gggttacaat gtctgcgaca acggttataa
     3301 aatggcttta atttatatcg tggtattaat cccgctgctt ttccagggtg cgggcggatg
     3361 gtcgctggat acgctgctga aaaaacggtt ttgcccccaa tgccgtctga agcaagattg
     3421 attcagtcgt ggaatctgac tttaaacatt ccaaccttat ctcgttaact tgatattctg
     3481 aaaaggaaat agaaatgaac aaaaacattg ctgccgcact cgccggtgct ttatccctgt
     3541 ctttggccgc cggtgcagtt gctgccaaca aaccggcaag caacgcaaca ggcgttcata
     3601 aatccgccca tggctcttgc ggcgcgtcca aatctgccga aggttcgtgc ggcgcggctg
     3661 gttctaaagc aggcgaaggc aaatgcggcg agggcaaatg cggtgcgacc gtaaaaaaaa
     3721 cccacaaaca caccaaagca tctaaagcca aggccaaatc tgccgaaggc aaatgcggcg
     3781 aaggcaaatg cggttctaaa taatcccccc ccttcaaacc aagccgcgtt tttcagtaaa
     3841 atgcggcttt tttaacggca aacaaagatt ttttaacaag cacatcattc ttttgtgcca
     3901 tccgaaccgg gtaaaaatat gattcaacac gcaggcttgg gctaccgccg cgacttggcg
     3961 gaagactttc tctcgctttc cgaaaacagc ccgatatgct ttatcgaagc cgcaccggaa
     4021 aactggctga aaatgggcgg ctgggcgcgc aaacagtttg accgtgtggc ggaacggctg
     4081 ccgctggcgt tgcacggatt gtctatgtcg ctgggcgggc aagcaccgct ggatactgat
     4141 ttgatagacg gcatcaaaga aatgatgcgc cgttacgatt gcacgttttt ctccgaccat
     4201 ttgagctact gccacgacgg cggtcatctt tacgatttgt tgccgctgcc cttcaccgag
     4261 gaaatggtgc atcatacggc gcggcgtatc cgtgaagtgc aagaccgttt gggctgccgc
     4321 atcgccgtgg aaaacacgtc ctactatctg cattccccgc ttgccgagat gaacgaggtc
     4381 gagttcctca acgccgtcgc acgtgaggcc gattgcggca ttcatctgga tgtgaacaat
     4441 atctacgtca acgccgtcaa tcacggcctg ttgtcgccgg aggctttttt gaaaaacgtt
     4501 gacgcagatc gcgtgtgcta tatccatatt gccggacatg acgtggaaac gccggaattg
     4561 ttgattgata cacatggcgc ggcagttttg ccgactgttt gggacttgct cgaacttgcc
     4621 tatgccaagc tgccgacgat tccgcccacc ctgttggaac gcgattttaa tttcccgcct
     4681 tttgccgaac tcgaagccga agtcgccaaa atcgccgatt atcaaacgcg tgccggaaag
     4741 gaataccgcc gtgcagcctg aaacctccgc ccaataccag caccgtttcg cccaagccat
     4801 acgcgggggc gaagccgcag acggtctgcc gcaagaccga ctgaacgtct atatccgcct
     4861 gatacgcaac aatatccaca gctttatcga ccgctgctat accgaaacgc ggcaatactt
     4921 tgacagcgaa gaatggagcc gtctgaaaga aggtttcgtc cgcgacgcgc acgcccaaac
     4981 gccctatttt caagaaatcc ccggcgagtt cctacaatat tgccaaagcc tgccgctttc
     5041 agacggcatt ttggcactga tggattttga atatacccaa ttgctggcag aagttgctca
     5101 aattccggat attcccgaca ttcattattc aaatgacagc aaatacacac cctcccctgc
     5161 ggcctttatc cggcaatatc gatatgatgt taccgatgat ttgcatgaag cggaaacagc
     5221 cttgttaata tggcgaaacg ccgaagatga tgtgatgtac caaacattgg acggcttcga
     5281 tatgatgctg ctagaaataa tgggttcctc cgcgctttcg tttgacaccc tcgcccaaac
     5341 ccttgtcgaa tttatgcctg aggacgataa ttggaaaaat attttgcttg ggaaatggtc
     5401 aggctggact gaacaaagga ttatcatccc ctccttgtcc gccatatccg aaaatatgga
     5461 aggcaattcc ccgggccaaa accatctatc cacataaaat taccttgttc ccgatactat
     5521 gccgctaccc gacctgaccg atgccgaatt aatagagtcg cgtaaactgc ttctgcattt
     5581 tgcgcggctt cagttgcccg accaccctga tttggctgaa gatttagtgc aggaaacatt
     5641 gctgtccgca tacagcgcag gcgacagttt tcaaggcagg gcacttgtca acagctggct
     5701 ttttgccata ttgaaaaaca aaattattga cgcattacgt caaatcggaa ggcagaggaa
     5761 agtctttacc acactggatg acgagctact ggatgaagca tttgaaagcc atttttccca
     5821 aaacgggcat tggacgccgg aagggcagcc gcaacattgg aacactccgg aaaaatcatt
     5881 aaacaacaac gaattccaaa aaattctgca aagctgccta tacaacttgc ctgaaaacac
     5941 cgcacgggta tttaccctga aggaaatact cggtttttca tccgacgaaa tacaacaaat
     6001 gtgcggtatc agcacgtcca actaccacac cattatgcac cgcgcccgag aatcattgcg
     6061 ccaatgcctg caaatcaaat ggttcaacca agaaaacccg aagtaaacgt tatgaaaaaa
     6121 tgccgcgata tcgccctgct tctttccaaa catcaggacc gggaaaccac cccgggcgag
     6181 aagatttcca tatacacaca cctgctgttc tgcccacatt gccgagaata taaaagacag
     6241 cttcaaacca tcaaaatatc actggcaaaa acaaccagaa cttcaaaata aatgccgtct
     6301 gaaaaggctt cagacggcat aagctgacgg aaaaaaatca aaccgattta ctgttatcgg
     6361 cagttcatcc ataatacacg cttcaaaagc agcatattcc tccatacgga atatataaat
     6421 acgcaaaaca cgaaggctgc atcaatttgc tttatttgcc ttatttcaca gacggcgcta
     6481 cccctcccgc ccaacccgtt ctttctgaat gagcagattt caatgattaa ggaaacccta
     6541 atgcgcccaa tcttcctatc tttcgtttta ctccctattt tgataaccgc ctgcagcaca
     6601 ccggacaagt ctgcccgatg ggaaaatatc ggcacaatct caaacggcaa tattcataca
     6661 tatatcaata aagacagcgt gagaaaaaac ggaaatctga tgattttcca agataaaaaa
     6721 gttgttacca atctaaaaca agaacgtttt gccaacaccc ccgcatacaa gactgccatt
     6781 gccgagtggg aaatccactg caacaacaaa acataccgct taagttcgct acagttgttt
     6841 gatacaaaaa acacggaaat ttccacacaa aactacacag cctcttccct ccgcccgatg
     6901 agcatcctgt ccgggacatt aaccgaaaaa caatatgaaa ccgtatgcgg aaaaaaactc
     6961 tgattgcaac ttatacacaa acttacccac aaaccttatc ataaaaatgc cgtctgaaat
     7021 actgaaatat cagcatttcg gacggcattt tgccatttct ccgaaaagtt atcctcgaag
     7081 ttatccacat tattttttta aaccagcttc catccgaaat ataaacctgc tatacaattt
     7141 cattatcaaa acgtaaattg ttccattgat acacaaaact gcttaccccc ataattttga
     7201 taaagcattt cttacattcc cggctccgtc ccgtaatcaa cac
//
LOCUS       DAXHTR010000109         7148 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.109, whole genome shotgun sequence.
ACCESSION   DAXHTR010000109 DAXHTR010000000
VERSION     DAXHTR010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7148
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.109"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            197..499
                     /locus_tag="ACFNML_001577"
     CDS             197..499
                     /locus_tag="ACFNML_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392929.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            783..935
                     /locus_tag="ACFNML_001578"
                     /pseudo
     CDS             783..935
                     /locus_tag="ACFNML_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
     gene            1092..2834
                     /gene="hrpB"
                     /locus_tag="ACFNML_001579"
     CDS             1092..2834
                     /gene="hrpB"
                     /locus_tag="ACFNML_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HFC6392930.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLSSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETASGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRFHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     SYETAIHAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLSGERGFYWQNTLGWHFHPRH
                     QFYLGADYGRVSGESAQYASGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            2927..>7148
                     /gene="hrpA"
                     /locus_tag="ACFNML_001580"
     CDS             2927..>7148
                     /gene="hrpA"
                     /locus_tag="ACFNML_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion exoprotein HrpA"
                     /protein_id="HFC6392931.1"
                     /translation="MNKGLHRTVFSKKHNTMVAVAETANSQGKGKQAGTSAPASPQIS
                     DGLYSKLKTTLKTLVCSLVSVSMVLPAHAQIITDKTAPKNQQAVILKTNTGAPLVNIQ
                     TPNARGLSHNRYTQFDVDAKGAVLNNDRNNNPFLAKGSAQLILNEVRGAASKLNGIVT
                     VGGQKADVIIANPNGITVNGGGFKNVGRGILTTGTPQIGKDGALTGFDVRQGTLTVGT
                     SGWNDKGGADYTEVLARAVALQGKLQGKNLAVSTGAQKVDYASGEISAGTAAGTKPTV
                     ALDTAALGGMYADSITLIANEKGVGVKNAGTLEAAKQLIVTSSGRIENSGRIATTADS
                     TEASPTYLSIETTETGAAGAFISNGGRIESKGLLVIDTGEDIGLRNGAVVQNNGNRPA
                     ATVLNAGRNLVIESKANVNNAKGSTNLSTGGRTVIKEASIQTGTTVYSSSKGNAELGN
                     NTRITGADVTVLSNGTISSSAVIDAKDTAHIEAGKPLSLEASTVTSDIRLNGGSIKGG
                     KQLALLADDNITAKTTNLNTPGNLYVHTGKDLNLNVDKDLSAASIHLKSDNAAHITGT
                     SKTLTASKDMGVEAGSLNVTNTNLRTNSGNLHIQAAKGNIQLRNTKLNAAKALETTAL
                     QGNIVSDGLHAVSADGHVSLLANGNADFTGHNTLTAKADVNAGSVGKGRLKADNTNIT
                     SSSGDITLVAGNGIQLGDGKQRNSINGKHISIKNNGGNADLKNLNVHAKSGALNIHSD
                     RALSIENTKLESTHNTHLNAQHERVTLNQVDAYAHRHLSITGSQIWQNDKLPSANKLV
                     ANGVLALNARYSQIADNTTLRAGAINLTAGTALVKRGNINWSTVSTKTLEDNAELKPL
                     AGRLNIEAGSGTLTIEPANRISAHTDLSIKTGGKLLLSAKGGNAGAPSAQVSSLEAKG
                     NIRLVTGETDLRGSKITAGKNLVVATTKGKLNIEAVNNSFSNYFPTQKAAELNQKSKE
                     LEQQIAQLKKSSPKSKLIPTLQEERDRLAFYIQAINKEVKGKKPKGKEYLQAKLSAQN
                     IDLISAQGIEISGSDITASKKLNLHAAGVLPKAADSEAAAILIDGITDQYEIGKPTYK
                     SHYDKAALNKPSRLTGRTGVSIHAAAALDDARIIIGASEIKAPSGSIDIKAHSDIVLE
                     AGQNDAYTFLKTKGKSGKIIRKTKFTSTRDHLIMPAPVELTANGITLQAGGNIEANTT
                     RFNAPAGKVTLVAGEELQLLAEEGIHKHELDVQKSRRFIGIKVGKSNYSKNELNETKL
                     PVRVVAQTAATRSGWDTVLEGTEFKTTLAGADIQAGVGEKARADAKIILKGIVNRIQS
                     EEKLETNSTVWQKQAGRGSTIETLKLPSFESPTPPKLSAPGGYIVDIPKGNLKTEIEK
                     LSKQPEYAYLKQLQVAKNINWNQVQLAYD"
BASE COUNT         2096 a         1759 c         1651 g         1642 t
ORIGIN      
        1 tttgtaaaag caaggatagg gcatctgccc ttttgattgt ttgattaacg atacaaggag
       61 tttcaaaatg agagttttat agtggattaa atttaaacca gtacagcgtt gcctcgcctt
      121 gccgtaccat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac
      181 tatatgtgtt catgaaatga cttgggtcgg aggctcaggt aatgcgcaac aaagttcata
      241 ttattgcgaa atttgcgaat ctgcagggct taacgatacg ggaaatcctg ataaatcttt
      301 aggattgcca aacaatacgt tcagtaatcc gcctggttgg ggagctacaa tcggagcttt
      361 agcaggtagc cgcataggga tgcctgaatt tggtacgttt gcgagccatg ccattgaaaa
      421 tttcgactgg tcatggtatc gacgttatag ggaaattgcc gaaacgattg aacgagaata
      481 ttcaggcggt ttgccttaat agttgaggag gtcatgatgt ttgccaaaca ttatcaattc
      541 atcgcactcg gcatcatgct gcttctttat atgttgattc tctatacgac cgatttttcc
      601 aatctgacgt attggatgct gttttttatc tgttttatta caggaaaaat attagctcgt
      661 ttgttagaga aaagctttaa ataaaatagc agctagtcgc aaaaggtcgt ctgaaacctt
      721 ttcaggcggc ctttctaaaa tacatccaac ttcctaatcc ctatttttca aaaaggaaat
      781 ctatgcccca tctgcaaaac ctgtctttgg gcttaaagaa aaagctgcct gttatcctgc
      841 aaacagaaat atcagaatgc ggcttggcat gtctggcggc tgtggcggga tttcatggtt
      901 tccatacgga tttacgcgca ctgcgttcaa aatactgtcc gagacctttg caaaattccc
      961 caaaatcccc taaatgtctt ggtgggaatt ttggggattt tggggaattt tgcaaaggtc
     1021 tcattctata actgtaaata cttttaaatt tatgacagaa tagtaaatat tgctaaaata
     1081 atattaatgt catgaaattt tttcctgctc catgtctgtt ggttatcctg gctgtcatac
     1141 cccttaaaac cttagctgcc gatgaaaacg atgcagaact tatccgttcc atgcagcgtc
     1201 agcagcacat agatgctgaa ttgttaactg atgcaaatgt ccgtttcgag caaccattgg
     1261 agaagaacaa ttatgtcctg agtgaagatg aaacaccgtg tactcgggta aattacatta
     1321 gtttagatga taagacggcg cgcaaatttt cttttcttcc ttctgtgctc atgaaagaaa
     1381 cagcttttaa aactgggatg tgtttaggtt ccaataattt gagcaggcta caaaaagccg
     1441 cgcaacagat actgattgtg cgtggctacc tcacttccca agctattatc caaccacaga
     1501 atatggattc gggaattctg aaattacggg tatcagcagg cgaaataggg gatatccgct
     1561 atgaagaaaa acgggatggg aagtctgccg agggcagtat tagtgcattc aataacaaat
     1621 ttcccttata taggaacaaa attctcaatc ttcgcgatgt agagcagggc ttggaaaacc
     1681 tgcgtcgttt gtcgagtgtt aaaacagata ttcagattat accgtccgaa gaagaaggca
     1741 aaagcgattt acagatcaaa tggcagcaga ataaacccat acggttcagt atcggtatag
     1801 atgatgcggg cggcaaaacg accggcaaat atcaaggaaa tgtcgcttta tcgttcgata
     1861 accctttggg cttaagcgat ttgttttatg tttcatatgg acgcggtttg gcgcacaaaa
     1921 cggacttgac tgatgccacc ggtacggaaa ctgcaagcgg atccagaagt tacagcgtgc
     1981 attattcggt gcccgtaaaa aaatggctgt tttcttttaa tcacaatgga catcgttacc
     2041 acgaagcaac cgaaggctat tccgtcaatt acgattacaa cggcaaacaa tatcagagca
     2101 gcctggccgc cgagcgcatg ctttggcgta acaggtttca taaaacttca gtcggaatga
     2161 aattatggac acgccaaacc tataaataca tcgacgatgc cgaaatcgaa gtgcaacgcc
     2221 gccgctctgc aggctgggaa gccgaattgc gccaccgtgc ttacctcaac cgctggcaac
     2281 tggacggcaa gttatcttac aaacggggta ccggtatgcg ccaaagtatg cccgcacctg
     2341 aagaaaacgg cggcggtact attccaggca catcccgtat gaaaatcata accgccggat
     2401 tggatgcagc ggccccgttt atgttgggca aacagcagtt ttcctacgaa accgctatcc
     2461 atgcacaatg gaacaaaacg cctttggttg cccaagacaa gttgtctatc ggcagccgct
     2521 acaccgttcg aggatttgac ggtgaacaga gcctttccgg agagcgtggt ttctactggc
     2581 agaacacttt gggctggcat ttccatccgc gccatcaatt ctatctcggc gcggactatg
     2641 gtcgggtatc gggcgaaagt gcccaatatg cctcgggcaa gcggctggcg ggtgcagtgg
     2701 ccggcttcag aggcagccat aaagtaggcg gtatatttgc ttatgactta tttgccggca
     2761 agccgctgca caagcccgaa ggttttcaga cggccaacac cgtttacggc ttcaatttga
     2821 attacagttt ctaaccctct cctgcatttt ttaccgacat tcagacagtc tttcatcatt
     2881 tacaggccgt ctgaagcgat tacaaacatc atttaaaggg ataaatatga acaaaggtct
     2941 gcatcgtacc gttttcagca aaaagcacaa cactatggtt gcagttgccg aaaccgccaa
     3001 cagtcaggga aaaggcaaac aggcaggaac ttccgctccg gcttcgccac aaatttcaga
     3061 cggcctttac agtaaactca aaaccacgct taaaaccttg gtctgctctt tggtttccgt
     3121 cagtatggta ttgcctgccc acgcacaaat cattaccgat aaaaccgcgc ccaaaaacca
     3181 gcaggccgtt attcttaaaa ccaataccgg tgcgccgttg gtcaacatcc aaactcctaa
     3241 tgcccgtgga ttgagccaca accgctatac ccagtttgat gtcgacgcta aaggtgctgt
     3301 actgaacaac gatcgcaaca acaacccgtt tctggccaaa ggcagtgcgc agttgatttt
     3361 gaacgaggtg cgcggtgcgg ccagcaaact caatggcatc gttaccgtag gtggtcaaaa
     3421 ggccgatgtg attatcgcca accccaacgg cattaccgtg aacggcggcg gctttaaaaa
     3481 cgtcggccgc ggtatcttaa ccaccggtac gcctcaaatc ggcaaagacg gtgccttgac
     3541 gggatttgac gtgcgccaag gcacattgac cgtcggtacg tcaggttgga acgacaaagg
     3601 cggagccgat tacaccgaag tgcttgcccg tgcggttgct ttgcagggga aattgcaggg
     3661 taaaaacctg gcggtttcga ccggcgcaca gaaagtagat tacgccagcg gtgaaatcag
     3721 cgcaggtacg gcagcgggta cgaaaccgac cgttgccctc gatactgccg cattgggtgg
     3781 tatgtacgcc gacagcatta ccctgattgc caatgaaaaa ggcgtgggtg tcaaaaatgc
     3841 gggcacactc gaagcggcca agcaattgat cgttacttcc tccggccgta ttgaaaacag
     3901 cggccgcatc gccaccactg ccgacagcac tgaagcatca ccgacttatc tctccatcga
     3961 aaccaccgaa acaggcgcag caggcgcatt tatctccaat ggcggccgga tagagagcaa
     4021 aggcttattg gttattgata ccggggagga catcggtttg cgtaacggag cggtcgttca
     4081 aaacaacggc aaccgccctg ctgctacggt attaaatgcc ggccgtaatt tggttatcga
     4141 aagcaaagca aatgtgaaca atgctaaagg ttcgaccaat ctgtcgaccg gcggccgtac
     4201 cgtcatcaag gaggccagta ttcagactgg cactaccgta tacagttcca gcaaaggcaa
     4261 cgccgaatta ggcaataaca cacgcattac cggggcagat gttaccgtat tatccaacgg
     4321 caccatcagc agttccgccg taatagatgc caaagacacc gcacacatcg aagcaggcaa
     4381 accgctttct ttggaagctt caacagttac ctccgatatc cgcttaaacg gaggcagtat
     4441 caagggcggc aagcagcttg ctttactggc agacgataac attactgcca aaactaccaa
     4501 tctgaatact cccggcaatc tgtatgttca tacaggtaaa gatctgaatt tgaatgttga
     4561 taaagatttg tctgccgcca gcatccattt gaaatcggat aacgctgccc atattaccgg
     4621 caccagtaaa accctcactg cctcaaaaga catgggtgtg gaggcaggct cgctgaatgt
     4681 taccaatacc aatctgcgta ccaactcggg taatctgcac attcaggcag ccaaaggcaa
     4741 tattcagctt cgcaatacca agctgaacgc agccaaggct ctcgaaacca ccgcattgca
     4801 gggcaatatc gtttcagacg gccttcatgc tgtttctgca gacggtcatg tatccttatt
     4861 ggccaacggt aatgccgact ttaccggtca caataccctg acagccaagg ccgatgtcaa
     4921 tgcaggatcg gttggtaaag gccgtctgaa agcagacaat accaatatca cttcatcttc
     4981 aggagatatt acgttggttg ccggcaacgg tattcagctt ggtgacggaa aacaacgcaa
     5041 ttcaatcaac ggaaaacaca tcagcatcaa aaacaacggt ggtaatgccg acttaaaaaa
     5101 ccttaacgtc catgccaaaa gcggggcatt gaacattcat tccgaccggg cattgagcat
     5161 agaaaatacc aagctggagt ctacccataa tacgcatctt aatgcacaac acgagcgggt
     5221 aacgctcaac caagtagatg cctacgcaca ccgtcatcta agcattaccg gcagccagat
     5281 ttggcaaaac gacaaactgc cttctgccaa caagctggtg gctaacggtg tattggcact
     5341 caatgcgcgc tattcccaaa ttgccgacaa caccacgctg agagcgggtg caatcaacct
     5401 tactgccggt accgccctag tcaagcgcgg caacatcaat tggagtaccg tttcgaccaa
     5461 gactttggaa gataatgccg aattaaaacc attggccgga cggctgaata ttgaagcagg
     5521 tagcggcaca ttaaccatcg aacctgccaa ccgcatcagt gcgcataccg acctgagcat
     5581 caaaacaggc ggaaaattgc tgttgtctgc aaaaggagga aatgcaggtg cgcctagtgc
     5641 tcaagtttcc tcattggaag caaaaggcaa tatccgtctg gttacaggag aaacagattt
     5701 aagaggttct aaaattacag ccggtaaaaa cttggttgtc gccaccacca aaggcaagtt
     5761 gaatatcgaa gccgtaaaca actcattcag caattatttt cctacacaaa aagcggctga
     5821 actcaaccaa aaatccaaag aattggaaca gcagattgcg cagttgaaaa aaagctcgcc
     5881 taaaagcaag ctgattccaa ccctgcaaga agaacgcgac cgtctcgctt tctatattca
     5941 agccatcaac aaggaagtta aaggtaaaaa acccaaaggc aaagaatacc tgcaagccaa
     6001 gctttctgca caaaatattg acttgatttc cgcacaaggc atcgaaatca gcggttccga
     6061 tattaccgct tccaaaaaac tgaaccttca cgccgcaggc gtattgccaa aggcagcaga
     6121 ttcagaggcg gctgctattc tgattgacgg cataaccgac caatatgaaa ttggcaagcc
     6181 cacctacaag agtcactacg acaaagctgc tctgaacaag ccttcacgtt tgaccggacg
     6241 tacgggggta agtattcatg cagctgcggc actcgatgat gcacgtatta ttatcggtgc
     6301 atccgaaatc aaagctccct caggcagcat agacatcaaa gcccatagtg atattgtact
     6361 ggaggctgga caaaacgatg cctatacctt cttaaaaacc aaaggtaaaa gcggcaaaat
     6421 catcagaaaa accaagttta ccagcacccg cgaccacctg attatgccag cccccgtcga
     6481 gctgaccgcc aacggtatca cgcttcaggc aggcggcaac atcgaagcta ataccacccg
     6541 cttcaatgcc cctgcaggta aagttaccct ggttgcgggt gaagagttgc aactgctggc
     6601 agaagaaggc atccacaagc acgagttgga tgtccaaaaa agccgccgct ttatcggcat
     6661 caaggtaggc aagagcaatt acagtaaaaa cgaactgaac gaaaccaaat tgcctgtccg
     6721 cgtcgtcgcc caaactgcag ccacccgttc aggctgggat accgtgctcg aaggtaccga
     6781 attcaaaacc acgctggccg gtgcggacat tcaggcaggt gtaggcgaaa aagcccgtgc
     6841 cgatgcgaaa attatcctca aaggcattgt gaaccgtatc cagtcggaag aaaaattaga
     6901 aaccaactca accgtatggc agaaacaggc cggacgcggc agcactatcg aaacgctaaa
     6961 actgcccagc ttcgaaagcc ctactccgcc caaattgtcc gcacccggcg gctatatcgt
     7021 cgacattccg aaaggcaatc tgaaaaccga aatcgaaaag ctgtccaaac agcccgagta
     7081 tgcctatctg aaacagctcc aagtagcgaa aaacatcaac tggaatcagg tgcagcttgc
     7141 ttacgaca
//
LOCUS       DAXHTR010000110         7024 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.110, whole genome shotgun sequence.
ACCESSION   DAXHTR010000110 DAXHTR010000000
VERSION     DAXHTR010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7024
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.110"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            124..753
                     /locus_tag="ACFNML_001581"
     CDS             124..753
                     /locus_tag="ACFNML_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HFC6392932.1"
                     /translation="MIQNVVTSIILYSGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNKNLIFLVAMITFASCGADNIGVFVPYFTTLNLANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGVYILIENNSFDMLWTVSGQEKIL"
     gene            899..2560
                     /locus_tag="ACFNML_001582"
     CDS             899..2560
                     /locus_tag="ACFNML_001582"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HFC6392933.1"
                     /translation="MLTYTPPDARPPAKTHEKPWLLLLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGIVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIGIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVLLPQNADAPQGWQTVWQGARPRNKDSKFALIRKIGENI"
     gene            2677..3282
                     /locus_tag="ACFNML_001583"
     CDS             2677..3282
                     /locus_tag="ACFNML_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HFC6392934.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LDHYDVKLGKSAAPLLKYAERIFQREAFIEALTPAEKAMRK"
     gene            3354..3746
                     /locus_tag="ACFNML_001584"
     CDS             3354..3746
                     /locus_tag="ACFNML_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFC6392935.1"
                     /translation="MPTSTKPYVLRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     EYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(3775..3924)
                     /locus_tag="ACFNML_001585"
     CDS             complement(3775..3924)
                     /locus_tag="ACFNML_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392936.1"
                     /translation="MPSEARIAGSDSIFNVPISNRQGQHGFNTPKQVLPINGFRLRPI
                     GQNAV"
     gene            complement(3945..4391)
                     /locus_tag="ACFNML_001586"
     CDS             complement(3945..4391)
                     /locus_tag="ACFNML_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HFC6392937.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSVKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTA
                     VDAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(4523..4735)
                     /gene="rpsU"
                     /locus_tag="ACFNML_001587"
     CDS             complement(4523..4735)
                     /gene="rpsU"
                     /locus_tag="ACFNML_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011484193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFC6392938.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(5024..6871)
                     /gene="ltgA"
                     /locus_tag="ACFNML_001588"
     CDS             complement(5024..6871)
                     /gene="ltgA"
                     /locus_tag="ACFNML_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase LtgA"
                     /protein_id="HFC6392939.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTHAPSSKTLADNIETAGLSAS
                     VPTRPAEPEGKTLADYSSYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKSL
                     GARRQWTLFAQEYAKLKPEGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLLE
                     QAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIGK
                     EARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQLT
                     DDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKLY
                     KQAVATGRNFYAVLAGEELGRKIDTRNNVPDAGKNSVLRMAEDGAIKRALVLFQNSRT
                     AGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTLR
                     YISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSHVGAQGLMQVMPATAREIA
                     GKIGMDAAQFYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTPL
                     EGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
BASE COUNT         1398 a         1868 c         1919 g         1839 t
ORIGIN      
        1 ctaaggcgag gcaacgccgt actggttttt gttaatccac tatattttga tggggttgaa
       61 gttgacatct acaaagttta atgtttgaat acattaaaaa aatatatttg aatgagatgt
      121 tttatgattc aaaatgttgt tacttcaata atcctgtatt ctgggacagc cgtagactta
      181 cttattatcc taatgttatt ttttgccaaa agaaaaagca gaaaagacat cattaacatc
      241 tatttaggac aatttctagg ctctgttagc ctgatattgc taagtttgct ttttgcattt
      301 gtcttagatt atattcctag taaagagatt ttaggtttgc tcggcttgat tccaattttc
      361 ctaggcatca aagttttgct gttaggagat tctgatggag aggctattgc caaagagggt
      421 ttgcgcaaag ataataaaaa cctgattttt ctagtcgcta tgattacttt tgcaagttgt
      481 ggtgctgaca atattggtgt ctttgttcca tattttacta ccttaaattt agcgaatttg
      541 atagtggctt tacttacctt tctagtcatg atttatctct tggttttttc tgcccaaaaa
      601 ttagcacaag tcccttctgt tggagaaact ttggaaaaat atagcagatg gtttgttgct
      661 gttgtttatt tgggattggg ggtatatatc ctgattgaaa acaacagttt tgatatgcta
      721 tggactgtgt cgggccagga aaaaatatta tgaaaaagat agtatctgca aagactgcca
      781 tgtgcagtct tttttgttgc cggtcttttt gtgtctgatg ccgtctgaag cagtctctgc
      841 acgacctttg tgcgaatatt tgctacactt ggcaatccgt ccatttcttc tcacgcttat
      901 gctgacctac actccgcccg atgcccgccc gcccgccaaa acccacgaaa agccgtggct
      961 gctgctgttg atggcgtttg cctggttgtg gcccggcgtg ttttcccacg atttgtggaa
     1021 tcctgccgaa cctgccgtct ataccgccgt cgaagcactg gcaggcagcc ccaccccttt
     1081 ggttgcccat ctgttcggtc aaaccgattt cggcataccg cccgtgtatc tttgggttgc
     1141 cgccgcgttc aaacatttgc tgtcgccgtg ggcagccgac ccgtatgatg ccgcacgctt
     1201 tgccggcgtg tttttcgccg ttgtcggact gacttcctgc ggctttgccg gtttcaactt
     1261 tttgggcaga caccacgggc gcagcgtcgt cctgattctc atcggctgta tcgggctgat
     1321 tccagttgcc catttcctca accccactgc cgccgccttt gccgccgccg gactggtgct
     1381 gcacggttat tctttggctc gccggcgcgt gattgccgcc tcttttctgc tcggtacggg
     1441 ctggacgctg atgtcgttgg cagcagctta tccggcagca tttgccctga tgctgccctt
     1501 gcccgtactg atgtttttcc gtccgtggca aagcaggcgt ttgatgttga cggcagtcgc
     1561 ctcacttgcc tttgccctgc cgcttatgac cgtttacccg ctgctcttgg caaaaacgca
     1621 gcccgcgctg ttcgcgcaat ggctcgacga tcacgttttc ggtacgttcg gcggcgtgcg
     1681 gcacgttcag acggcattca gtttgtttta ctatctgaaa aacctgcttt ggtttgcatt
     1741 gcctgcgctg ccgctggcgg tttggacggt ttgccgcacg cgcctgtttt cgaccgactg
     1801 ggggattttg ggcattgtgt ggatgcttgc cgttttggtg ctgcttgccg tcaatccgca
     1861 gcgttttcag gataacctcg tctggctgct tccgccgctt gccctgttcg gcgcggcgca
     1921 actggacagc ctgaggcgcg gcgcggcggc gtttgtcaac tggttcggca ttatggcgtt
     1981 cggactgttt gccgtgttcc tgtggacggg ctttttcgcc atgaattacg gctggcccgc
     2041 caagcttgcc gaacgcgccg cctacttcag cccgtattac gttcccgaca tcgatcccat
     2101 tccgatggcg gttgccgtac tgttcacacc cttgtggctg tgggcgatta cccgcaaaaa
     2161 catacgcggc aggcaggcgg ttaccaactg ggcggcaggc gttaccctga cctgggcttt
     2221 gctgatgacg ctgttcctgc cgtggctgga cgcggcgaaa agccacgcgc cggtcgtccg
     2281 gagtatggag gcatcgcttt ccccggaatt aaaacgggag ctttcagacg gcatcgagtg
     2341 tatcggcata ggcggcggcg acctgcacac gcggattgtt tggacgcagt acggcacatt
     2401 gccgcaccgc gtcggcgatg tacaatgccg ctaccgcatc gtcctcctgc cccaaaatgc
     2461 ggatgcgccg caaggctggc agacggtttg gcagggtgcg cgtccgcgca acaaagacag
     2521 taagttcgca ctgatacgga aaatcgggga aaatatataa aaaacaacag attgagccga
     2581 atttctggat taagtgccgg aaatcgcgta taattgcgcg attaaacctt tataaatcag
     2641 ccgttttaca ggcatcacac aggagcgaca attattatga tgaccctcta ttccggcatt
     2701 acctgcccct tcagccaccg ctgccgcttc gttttgtacg aaaaaggcat ggattttgaa
     2761 atcaaagacg tcgatattta caacaagccc gaagacctcg ccgtcatgaa cccgtataac
     2821 caagttcccg tgctggtcga gcgcgatttg gtgctgcacg agtccaacat catcaacgaa
     2881 tacatcgacg aacgcttccc ccatccgcag cttatgcccg gtgatcccgt tatgcgcggc
     2941 aggggccggc tggtgctgta ccgtatggaa aaagaattgt tcaaccacgt ccaagttttg
     3001 gaaaaccccg ccgccaccaa caaggaacag gcaaaagcgc gcgaagccat cggcaacggt
     3061 ctgaccatgc ttgccccttc gttcagcaaa agcaaataca tcctcggcga agatttttct
     3121 atgattgatg tcgcccttgc tccgctgctg tggcggctcg accactacga tgtcaaactg
     3181 ggcaaaagtg ccgcgccgct gctcaaatac gccgagcgca tcttccagcg cgaagccttt
     3241 atcgaagcac tgacacccgc cgaaaaagcc atgcgcaaat aagtccgaaa tgcttgcaaa
     3301 acccaccgtt ttgcaggcat tttcctattt tggcgtacaa cacggaaccc atcatgccca
     3361 cctcaaccaa accctacgtc ctccgcgctc tctgcgaatg gtgcagcgac aacagcctca
     3421 caccgcacat ccttgtttgg gtcaacgaac acacgcgcgt ccccatgcag tatgtccgcg
     3481 acaatgaaat tatgctcaac atcggtgcga ccgccacgca aaaccttcaa atcgacaacg
     3541 attggatcag cttttccgcc cgtttcggcg gacaggcgca cgacatatgg atacctgtcg
     3601 gacacgtcct cagccttttc gcacgggaga ccggcgaagg tatggggttt gaattggagg
     3661 agtaccgccc cgacacgccg cctgaaaaca cctctgccga aaccgcgccc cgacccgcca
     3721 aaaaaggctt gaaattggtc aaataaatct atgccgtctg aacggaatcg tgtttcagac
     3781 ggcattttgt ccgatggggc gcaaacggaa tccgtttatc ggcaaaacct gtttcggcgt
     3841 attgaaaccg tgttgcccct gccgattcga tattgggaca ttgaaaatgc tgtctgaacc
     3901 tgcgatacgg gcttcagacg gcattttgtc cgatattcgg gcaatcaggc ggtcagcacg
     3961 gctttcagga ttttgttgac ttcgcccata tcggctttgc cggcaagtcg ggttttcaat
     4021 acgcccatca ctttgcccat atccgccata cctgccgcgc cggtttcggc aacggcggca
     4081 tcgaccgcag tacggatttc gccggcggag agcatttgcg gcaggtagcg gtgcagtacc
     4141 tcgatttcgg cgttttcttt gtctgccaaa tcctgacggc cggcttcagt gtagattttc
     4201 acgctgtctt tgcgctgttt gaccattttg gtcaggatgg cggtgatttt ggcatcgtcg
     4261 gcttcggtgc gttcgtccac ttcaaactgt ttgacggcgg cgttgatgag gcggatggtg
     4321 ccgagggaaa cttggtcttt ggcgcgcatc gcggttttca tgtcttcggt aaggcggatt
     4381 ttcaggctca taatgttctc gctgggatgt cggatggaaa cggcgggttt cgatgccgtc
     4441 tgaaaagcaa aacacaccgc agagcaggtc ttgcggtgtg tcctcatatc acagggctga
     4501 cctgtaatct gtacttgaac gtttagtaca ttttgggcgg cagttgttgg ctgcgcaggc
     4561 gtttttgcag gcgttttacg gctgccgctt ttttgcgttt gcgttcggta gtcggttttt
     4621 cgtaggcttc gcgggcgcgc agctcggtca gcaggccggt tttttctacg gcgcgtttga
     4681 aacggcgcat agcgacttca aagggttcgt tttcttttac gcggattgca ggcattttat
     4741 ttcctttaat aaattctgtt gttgcatctg cccatcatat cggtggaaag ggtagggcag
     4801 acggtgttga aggtttaccg tatcgctgtg ccttgcggcg gcgggatacg cgccgtgacg
     4861 gaaacatcac ctcctaacgg gtcggcttgt gccgtgtcaa gctgcttgaa aaagcaggaa
     4921 aaacaatttt cggattgtct tatatttatg gttcgccgtc aatgccgttc gggataaaaa
     4981 tgccgtctga aagaccgggc gggtttcaga cggcatcggt acgtcagcgt gcaggaacaa
     5041 tgcccatacg ctgtttgagc gggatgtgcg gcgcgccgaa gagggcggcg tagtaggcgg
     5101 cattggtcat cacttttttg acatagtcgc gcgtttcgga aaacgggatg gtttcggcat
     5161 ataccgcgcc ttcgaggggc gtgtccgcct gccatcggcg cgccctgccg ggaccggcgt
     5221 tatagcctgc ggtggcgagg acttcgttgt tttgcaggcg gcgtttggtg tccgccatat
     5281 accacgtccc catacggata ttgccgtcgg cggtgtaaaa ttgtgcggca tccataccga
     5341 ttttgccggc gatttcgcgc gcggtggcag gcataacctg catcagcccc tgcgcgccta
     5401 cgtgggattg cgcgcctata acgaagcggc tttcctgacg aatcagaccg taaacccaag
     5461 ccggatcgac attaacattt tgcgcgtggc ggattaccgt gtctttaaac ggcgaaatat
     5521 agcgcaaggt gtagttgagt ttgcggtcgg tgcgttccgc gctgttgacc gccatatcgt
     5581 aaaaaccgtg gtcgaacgcg gtttgcgcgg cggtcagcag cttgtcttcg tcaaagccgc
     5641 gtgtggcaaa acgccattcc gcctgagcct gacggcgcat tttcgcatcg ccggcggttc
     5701 ggctgttttg gaacagtacc agcgcgcgct taatcgcgcc gtcttccgcc atacggagga
     5761 cgctgttttt gccggcatcg ggaacattgt tgcgcgtatc gattttgcgc cccaactctt
     5821 cccctgccag tacggcataa aaattcctgc ccgtcgctac cgcctgtttg tagagttttt
     5881 ccgcctcttg cgtgttgccc gttgcggcgc ggctgcgtgc cagccagtag agccaggtcg
     5941 ggcttttttg cagtttttcg ggcatatgcg agattacgga agccagctcg tcccaacgtc
     6001 gggcgcgcaa ggcggcgcgg gcgtaccact cgatttggtc gtcggtcagt tggcggcggt
     6061 cggcaacctt gccgtaatag tccaaggcgg caggcacatt gaggttttgc gactgataat
     6121 gccccaatac gccccacgcg aaactgcgtt gttcgaggct taaaccgctt tccatttcgg
     6181 acagcagggc ggcggcattc ggcgatttgc gtgcttcttt gccgatgacg ttcaacaggg
     6241 catattcgcg cgaaccttgt gtaccgccgt caaacgggct gcccaatgcg gcggcaaggt
     6301 tgcgtgcgtc tgtggtttgg cggccggcca gcagtccgcg cacgcgcctc caggcgtcgt
     6361 tgccgtccaa caagccggat gcggctgcct gttccaacag tttggtgcag cccgaaggca
     6421 gtttgcccgt atttttgacc agttcggcgg cacgcgtata gtcgttgcgg ctcgaatcgg
     6481 cgtagcattc gacttcttgg gcgcgccctt caggtttgag tttggcatat tcctgtgcaa
     6541 acagcgtcca ctgtctgcgt gcgcccaaag acttcagcca ctcgttgcgg acattttccg
     6601 ccatcgcgct gtcgccggcg ttttccaaat aggcggcgac ggcggcatcg tttttctgtt
     6661 tcactgcatc cagtgcggaa gggtagctgc tgtaatctgc cagcgttttt ccttcgggtt
     6721 cggcagggcg ggtgggaacg cttgccgaaa gaccggcagt ttctatattg tctgccaggg
     6781 tcttgctgct gggcgcgtgt gtcgaagaac acgcggcaag caccagggcc gccagcagcg
     6841 gcagggaatg cttcatagag ggtaggtaca tcggatttcc ttaagaatcg gaaccctgaa
     6901 cggtcagggt tggaaaagac aaaatgccgt ctgaacaggc gtttgcccga attatatgcc
     6961 gaaactgcac cgcctttgga atgtttccga cataatttat agtggattaa atttaaacca
     7021 gtac
//
LOCUS       DAXHTR010000111         6951 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.111, whole genome shotgun sequence.
ACCESSION   DAXHTR010000111 DAXHTR010000000
VERSION     DAXHTR010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6951
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.111"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="ACFNML_001589"
     CDS             complement(78..1424)
                     /gene="mnmE"
                     /locus_tag="ACFNML_001589"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFC6392940.1"
                     /translation="MSDNVPTIAAVATAPGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGVNDKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGEGEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1555..1710
                     /locus_tag="ACFNML_001590"
     CDS             1555..1710
                     /locus_tag="ACFNML_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392941.1"
                     /translation="MEKRNTYAFLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1707..2138
                     /locus_tag="ACFNML_001591"
     CDS             1707..2138
                     /locus_tag="ACFNML_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFC6392942.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGS
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2272..6642
                     /gene="app"
                     /locus_tag="ACFNML_001592"
     CDS             2272..6642
                     /gene="app"
                     /locus_tag="ACFNML_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HFC6392943.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFTYKIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASAESHFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAGKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDSALAKQREAETQPATTAFP
                     RARRARRDLPQPQPQPQPQPQPQRDLISRYANSGLSEFSATLNSVFAVQDELDRVFAE
                     DRRNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTF
                     DDGIGNSARLAHGAVFGQYGIGRFDIGISTGAGFSSGSLSDGIGSKIRRRVLHYGIQA
                     RYRAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQ
                     HISITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAA
                     AKGPQLEAQHSAGIKLGYRW"
BASE COUNT         1856 a         2007 c         1684 g         1404 t
ORIGIN      
        1 accaaacgga tcggattccc gtttttatgg gataacggaa tgttcagccg gacaccgcca
       61 cgatgctttc gatccgttta ttttccgata caaaacctcg aaaaaatcac gccgagcagg
      121 tcgtccgccg taaactcgcc cgtgatttcg ccgcatgcga cctgcgccaa gcgcaagtgt
      181 tcggcaaaca gctcgatttg atggttgccg cacaatgccg ccagcgacaa ttcttcctgc
      241 gctgctttga gtgcgttgac gtgccgcgtc cgcgccaaaa acagcccttc gccctcgccc
      301 tgccatccgg cctcgcgcaa caacgtccgt ttcagcgcgt ccaagccgtc gccggttttc
      361 gccgacagcg cgatgacggt ttccgcgccc gtaccgaacc cgcctgccgc gtgtgcgtgc
      421 aaatcggatt tgctgtggat ttcgatgcgt ttcaactccg gcggcaacgc gtccaaaatc
      481 gcccgtgtct tgtcgttcac gccttcgcgc ggatcgacca acaccagcgc gacatcggct
      541 tcggatacgg ctttgcggct gcgttcgatg ccgatgcgtt cgaccacgtc gtccgtctcg
      601 cgcaaacctg ccgtatcgac aatatgcacc ggaacgccgt caatcaggat acgttccctg
      661 accgcgtcgc gcgtcgttcc ggcaatatcg gtaacaatcg ccacttcgtc gcccgccagc
      721 gcgttcaaca ggctggattt gccgacattc ggcgcgccga ccaatacgac attcagacct
      781 tcgcgcaaaa tcgcgccctg ctgcgcgttg gcaagcacat catccacggc gcggcgcaag
      841 ccgtccagtt tgccgcgtgc gtctgccgct tcgagaaaat caatgtcttc ctcgggaaaa
      901 tctaacgtcg cttcgaccag catccgcaag gtaatcaagt cttcgaccag accgtgtatc
      961 cgccgcgaaa aatcgccttt gagcgagcgc aacgccagac gcgccgccga acggctggat
     1021 gcgtcaatca aatccgccac gccttccgcc tgtgccaagt ccagtttgtc gtttaaaaac
     1081 gcacgcttcg taaactcgcc cggttcggca aggcgcgcgc ccaattccaa acagcggttc
     1141 agcagcatat ccatcactac cggcccgccg tgtccctgaa gctcgataac atcttcgccc
     1201 gtaaaacttg ccggtgcggc aaaaaaaagc aaaagcccgc tgtcgattgc ctgaccgtcc
     1261 gcgtccgtaa aatcagcata ggttgccacg cgcggcttgg gcgttttccc acacaaagcc
     1321 tctgccatcg gcagcaggtt tttccccgat atgcgtatca cgcccacgcc gccgcgcccg
     1381 ggtgcggtag cgactgccgc aatcgttgga acgttatccg acataaaacc cccgaaaatt
     1441 caaaacagcc gcgattatag caaatgccgt ctgaagtccg acggtttggc tttcagacgg
     1501 cataaaaccg caaaaatgct tgataaatcc gtccgcctga cctaatataa ccatatggaa
     1561 aaacgaaaca catacgcctt cctgctcggt ataggctcgc tgctgggtct gttccatccc
     1621 gcaaaaaccg ccatccgccc caatcccgcc gacgatctca aaaacatcgg cggcgatttt
     1681 caacgcgcca tagagaaagc gcgcaaatga ccgaaaacgc acaggacaag gcgcggcagg
     1741 ctgtcgaaac cgtcgtcaaa tccccggagc ttgtcgagca aatcctgtcc gacgagtacg
     1801 tgcaaataat gatagcccgg cgtttccatt cgggatcgtt gccgccgccg tccgacttgg
     1861 cgcaatacaa cgacattatc agcaacgggg cagaccgcat tatggcaatg gcggaaaaag
     1921 aacaagccgt ccggcacgaa accatacggc aagaccaaac cttcaacagg cgcgggcaac
     1981 tgtacggctt catcagcgtc atcctgatac tgctttttgc cgtcttcctc gtatggagcg
     2041 gctaccccgc aaccgccgcc tcccttgccg gcggcacagt ggttgccttg gcgggtgctt
     2101 tcgtgattgg aagaagccga gaccaaggca aaaattaatg acaaatccta gggcgtgctt
     2161 catatccgcc cgaacgccga accgcacata taggcacatc ccgcgcgccg cccggaagcg
     2221 gaagccgcgc cctcccaaac aaacccgaat cccgtcagat aaggaaaaat aatgaaaaca
     2281 accgacaaac ggacaaccga aacacaccgc aaagccccga aaaccggccg catccgcttc
     2341 tcgcctgctt acttagccat atgcctgtcg ttcggcattc ttccccaagc ttgggcggga
     2401 cacacttatt tcggcatcaa ctaccaatac tatcgcgact ttgccgaaaa taaaggcaag
     2461 tttgcagtcg gggcgaaaga tattgaggtt tacaacaaaa aaggggagtt ggtcggcaaa
     2521 tcaatgacaa aagccccgat gattgatttt tctgtggtgt cgcgtaacgg cgtggcggca
     2581 ttggtgggcg atcaatatat tgtgagcgtg gcacataacg gcggctataa caacgttgat
     2641 tttggtgcgg aaggaagcaa tcccgatcaa catcgtttta cttataaaat tgtgaaacgg
     2701 aataattata aagcagggac taacggccat ccttatggcg gcgattatca tatgccgcgt
     2761 ttacataaat ttgtaaccga tgcagaacct gttgaaatga ccagttatat ggatgggcgg
     2821 aaatatatcg atcaaaataa ttaccctgac cgtgttcgta ttggggcagg caggcaatat
     2881 tggcgatctg atgaagatga gcccaataac cgcgaaagtt catatcatat tgcaagtgcg
     2941 tattcttggc tcgttggtgg caataccttt gcacaaaatg gatcaggtgg tggcacagtc
     3001 aacttaggta gtgaaaaaat taaacatagc ccatatggtt ttttaccaac aggaggctca
     3061 tttggcgaca gtggctcacc aatgtttatc tatgatgccc aaaagcaaaa gtggttaatt
     3121 aatggggtat tgcaaacggg caacccctat ataggaaaaa gcaatggctt ccagctggtt
     3181 cgtaaagatt ggttctatga tgaaatcttt gctggagata cccattcagt attctacgaa
     3241 ccacgtcaaa atgggaaata ctcttttcac gacaataata atggcacagg aaaaatcgac
     3301 gccaaacatg aacacaattc tctgcctaat agattaaaaa cacgaaccgt tcaattgttt
     3361 aatgtttctt tatccgagac agcacaagaa cctgtttatc atgctgcagg tggtgtcaac
     3421 agttatcgac ccagactgaa taatggagaa aatatttcct ttattgacga aggaaaaggc
     3481 gaattgatac ttaccagtaa catcaaccaa ggtgctggag gattatattt ccaaggagat
     3541 tttacggtct cgcctgaaaa caacgaaacg tggcaaggcg cgggcgttca tatcagtgaa
     3601 gacagtaccg ttacttggaa agtcaatggc gtggcaaacg accgcctgtc caaaatcggc
     3661 aaaggcacgc tgcacgttca agccaaaggg gaaaaccaag gctcgatcag cgtgggcgac
     3721 ggtaaagtta ttttagatca acaagcagat gaaaataata aaaaacaagc ctttagtgaa
     3781 atcggcttgg tcagcggcag gggtacggtg caactgaatg ccgataatca gttcaacccc
     3841 gacaaactct atttcggctt tcgcggcgga cgtttggatt tgaacgggca ttcactttcg
     3901 ttccaccgta ttcaaaatac cgatgaaggg gcgatgattg tcaaccacaa tcaagacaaa
     3961 gaatccaccg ttaccattac aggcaataaa gatattgcta caaccggcaa taacaacagc
     4021 ttggatagca aaaaagaaat tgcctacaac ggttggtttg gcgagaaaga tacgaccaaa
     4081 acgaacgggc ggctcaacct tgtttaccag cccgctgcag aagaccgcac cctgctgctt
     4141 tccggcggaa caaatttaaa cggcaacatc acgcaaacaa acggcaaact gtttttcagc
     4201 ggcagaccaa caccgcacgc ctacaatcat ttaaacgacc attggtcgca aaaagagggc
     4261 attcctcgcg gggaaatcgt gtgggacaac gactggatca accgcacatt taaagcggaa
     4321 aacttccaaa ttaaaggcgg acaggcggtg gtttcccgca atgttgccaa agtgaaaggc
     4381 gattggcatt tgagcaatca cgcccaagca gtttttggtg tcgcaccgca tcaaagccac
     4441 acaatctgta cacgttcgga ctggacgggt ctgacaaatt gtgtcgaaaa aaccattacc
     4501 gacgataaag tgattgcttc attgaccaat accgacatca gcggcaatgt cagccttgcc
     4561 gatcacgctc atttaaatct cacagggctt gccacactca acggcaatct tagtgcaaat
     4621 ggcgatacac gttatacagt cagccacaac gccacccaaa acggcgacct tagcctcgtg
     4681 ggcaatgccc aagcaacatt taatcaagcc acattaaacg gcaacacatc ggcttcgggc
     4741 aatgcttcat ttaatctaag cgaccacgcc gtacaaaacg gcagtctgac gctttccggc
     4801 aacgctaagg caaacgtaag ccattccgca ctcaacggta atgtctccct agccgataag
     4861 gcagtattcc attttgaaag cagccgcttt accggacaaa tcagcggcag caaggatacg
     4921 gcattacact taaaagacag cgaatggacg ctgccgtcag gcacggaatt aggcaattta
     4981 aaccttgaca acgccaccat tacactcaat tccgcctatc gccacgatgc ggcaggggcg
     5041 caaaccggca gtgcgacaga tgcgccgcgc cgccgttcgc gccgttccct attatccgtt
     5101 acacctccgg cttcggcaga atcccatttc aacacgctga cggtaaacgg caaattgaac
     5161 ggtcagggaa cattccgctt tatgtcggaa ctcttcggct accgcagcga caaattgaag
     5221 ctggcggaaa gttccgaagg cacttacacc ttggctgtca acaataccgg caacgaacct
     5281 gcaagcctcg aacaattgac ggtagtggaa gggaaagaca acaaaccgct gtccgaaaac
     5341 cttaatttca ccctgcaaaa cgaacacgtc gatgccggcg cgtggcgtta ccaactcatc
     5401 cgcaaagacg gcgagttccg cctgcataat ccggtcaaag aacaagagct ttccgacaaa
     5461 ctcggcaagg cagaagccaa aaaacaggcg ggaaaagaca acgcgcaaag ccttgacgcg
     5521 ctgattgcgg ccgggcgcga tgccgtcgaa aagacagaaa gcgttgccga accggcccgg
     5581 caggcaggcg gggaaaatgt cggcattatg caggcggagg aagagaaaaa acgggtgcag
     5641 gcggataaag acagcgcctt ggcgaaacag cgcgaagcgg aaacccagcc ggctaccacc
     5701 gccttccccc gcgcccgccg cgcccgccgg gatttgccgc aaccgcagcc ccaaccgcaa
     5761 cctcaacccc aaccgcagcg cgacctgatc agccgttatg ccaatagcgg tttgagtgaa
     5821 ttttccgcca cgctcaacag cgttttcgcc gtacaggacg aattggaccg cgtgtttgcc
     5881 gaagaccgcc gcaacgccgt ttggacaagc ggcatccggg acaccaaaca ctaccgttcg
     5941 caagatttcc gcgcctaccg ccaacaaacc gaccttcgcc aaatcggtat gcagaaaaac
     6001 ctcggcagcg ggcgcgtcgg catcctgttt tcgcacaacc ggaccgaaaa caccttcgac
     6061 gacggcatcg gcaactcggc acggcttgcc cacggcgccg ttttcgggca atacggcatc
     6121 ggcaggttcg acatcggcat cagcacgggc gcgggtttta gcagcggcag tctttcagac
     6181 ggcatcggaa gcaaaatccg ccgccgcgtg ctgcattacg gcattcaggc acgataccgc
     6241 gccggtttcg gcggattcgg catcgaaccg cacatcggcg caacgcgcta tttcgtccaa
     6301 aaagcggatt accgctacga aaacgtcaat atcgccaccc ctggccttgc gttcaaccgc
     6361 taccgcgcgg gcattaaggc agattattca ttcaaaccgg cgcaacacat ttccatcacg
     6421 ccttatttga gcctgtccta taccgatgcc gcttcgggca aagtccgaac gcgcgtcaat
     6481 accgccgtat tggctcagga tttcggcaaa acccgcagtg cggaatgggg cgtaaacgcc
     6541 gaaatcaaag gtttcacgct gtccctccac gctgccgccg ccaaaggccc gcaactggaa
     6601 gcgcaacaca gcgcgggcat caaattaggc taccgctggt aaccgccgga tatgccgaaa
     6661 ggggtctgac gatgccaccg tgcgctgtca aacccttttt ctgccgccgc cttgtgcctg
     6721 ctccattgtc tgataaccgt caaattgatt tttagcccat gtttggtggg tcggggaaat
     6781 ctatatcttc gtccgtgccg aaatagtctg agacctttgc aaaattcctt tccctcccga
     6841 cagccgaaac ccaaacacag gttttcgtct attttcgccc caaatacctc ctaattctac
     6901 ccaaataccc ccttaatcct ccccggacac ctgataatca ggcatccgtg c
//
LOCUS       DAXHTR010000112         6929 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.112, whole genome shotgun sequence.
ACCESSION   DAXHTR010000112 DAXHTR010000000
VERSION     DAXHTR010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6929
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.112"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..266
                     /locus_tag="ACFNML_001593"
     CDS             <1..266
                     /locus_tag="ACFNML_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC6392944.1"
                     /translation="CPIKPKPTNHAQQRKEEAKNDSHRSVGDSNRVVREGKQYLDSDT
                     GNHVYVKGDKVVILTPDGRQVTQFKNSKANTSKRVKNGKWTPK"
     gene            244..525
                     /locus_tag="ACFNML_001594"
     CDS             244..525
                     /locus_tag="ACFNML_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392945.1"
                     /translation="MGNGHQNNRSPLEKRIFYLEHSGQYLMICALSDYSQNKHTVVMA
                     NFLYPNEKMDWRNLDDLFNELVLEELQSSFMDWYPTIEEAISHHLEDFS"
     gene            904..2037
                     /locus_tag="ACFNML_001595"
     CDS             904..2037
                     /locus_tag="ACFNML_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392946.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NTNAFVAKLARLFRNADRAVVIVKESMRTEESLAGTVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGEEVWLDYYIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGYGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDSVFYQNMRELMTRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQSFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     VREEKQGDRLPDFPLNLEDLDKEVRRYAEAAARRSGGRRDLSH"
     gene            complement(2124..2399)
                     /locus_tag="ACFNML_001596"
     CDS             complement(2124..2399)
                     /locus_tag="ACFNML_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392947.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDTNLNGLNLWMKKGVENLWDDTVGKKARLMCISVFWIVVFNL"
     gene            complement(2416..3189)
                     /locus_tag="ACFNML_001597"
     CDS             complement(2416..3189)
                     /locus_tag="ACFNML_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6392948.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEQTSFNNPEPMTGFE
                     HTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINLIEISVYYKKTDQGW
                     VLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERLLTYGVKKMPSAYPE
                     YEAYEDKRHIPENPYFHEFYYIKKGENPAIITHWNNRVNQAEEDNYSTSVGSCINGFT
                     VQYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNSSKK"
     gene            complement(3575..3850)
                     /locus_tag="ACFNML_001598"
     CDS             complement(3575..3850)
                     /locus_tag="ACFNML_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6392949.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALKQEIAVKKLTAAQKRKLWEQAEKMPSET"
     gene            complement(3847..5130)
                     /locus_tag="ACFNML_001599"
     CDS             complement(3847..5130)
                     /locus_tag="ACFNML_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6392950.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFASGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRVAAL
                     IPGSLSLVLADRMPWPEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFILALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(5286..6704)
                     /gene="glnA"
                     /locus_tag="ACFNML_001600"
     CDS             complement(5286..6704)
                     /gene="glnA"
                     /locus_tag="ACFNML_001600"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HFC6392951.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDATVVLTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGETAYFGPEPEFFVFDGIEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPTVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
BASE COUNT         1872 a         1640 c         1674 g         1743 t
ORIGIN      
        1 cgtgtccgat caaaccaaaa ccaaccaatc atgcccaaca aagaaaagaa gaagctaaaa
       61 acgattctca tcgaagtgtg ggagattcca atcgtgtcgt tcgcgaagga aagcaatatt
      121 tagattccga cacaggaaac catgtttatg taaaaggaga taaagtggtt attctaactc
      181 ctgatggaag acaggtaact caatttaaga actcgaaagc caatacgtca aaaagggtaa
      241 aaaatgggaa atggacacca aaataatcga tctcccttag aaaaaagaat tttttatctg
      301 gagcattcgg ggcagtatct gatgatttgc gccctatcag attactccca aaataaacat
      361 actgtcgtta tggcgaattt tctctatcca aatgaaaaaa tggattggag aaatttagat
      421 gatttgttca atgagttggt tttagaggaa ttacagtctt catttatgga ttggtatccg
      481 actattgaag aggcaattag ccatcattta gaagactttt cgtaacaagc ataaattaaa
      541 ggttgtctga aactatgttc gattttcgac gacattttcc tcattggagg gggcggcaat
      601 ttatggaaaa tagatttgaa aaaatcgtta aggatatttt tagttttacc caatttgttc
      661 cgaagcagaa aatgagattc ccaccccatc ctcccgttat gacgactgtt ggtgggaatt
      721 ggaaatcttg aatgcctctg catttgaatt agtcaaaaaa ttgaatcaaa gcaaacaaga
      781 gtaacaagct gcagcctgca taggggtttt taaagtacgg cagcaaccgt agattaaatt
      841 aaaaatcaaa caatcaaagc tcgtctgata taatcgatat attctgttaa acgaaaaata
      901 aatatgttta aaaaattcaa accggtactg ttgtcatttt ttgcacttgt atttgccttt
      961 tggctgggga cgggtattgc ctatgagatt aatccgcgtt ggtttttgag cgatacggca
     1021 actgaaatac ctgaaaatac gaatgctttt gtggcgaaac ttgcccgcct gttccgaaat
     1081 gccgacaggg cggttgtcat cgtgaaggaa tcgatgagga cggaggaaag tcttgccgga
     1141 actgtggatg acggtccgtt gcagtcggag aaggattatc tcgcgctcgc tatccggctc
     1201 agtcgtttga aagaaaaggc gaaatggttt cacgtaacgg agcaggaaca tggggaagag
     1261 gtttggctgg attactatat cggcgagggc ggtttggttg cggtttcgct ttcgcaacgc
     1321 tcgccggaag cgtttgttaa tgccgaatat ctgtatcgga acgatcgtcc gttttctgta
     1381 aatgtgtacg gcggaacggc tcacggagaa aattatgaaa cgacaggaga atatcgggtt
     1441 gtttggcaac cggacggttc ggtatttgat gcgtcgggat acgggaaaat cggggaagat
     1501 gtttatgagc attgcctcgg gtgttatcag atggcccagg tatatttggc gaaataccgg
     1561 gatgtcgcga atgatgagca gaaggtttgg gacttccgcg aagagagcaa ccggattgcg
     1621 tcggactcgc gcgattctgt gttttatcag aatatgcggg aattgatgac ccgggggatg
     1681 aaggcgaaca gtcttgtggt cggctatgat gcggacggtc tgccgcaaaa agtctattgg
     1741 agtttcgaca atgggaaaaa acgccagagt ttcgaatatt atttgaaaaa cggaaatctt
     1801 tttattgcac aatcttcgac ggtagcattg aaagcggatg gcgtaacggc ggatatacag
     1861 acctatcatg cgcaacagac gtggtatttg gatggcgggc ggattgtccg cgaagagaaa
     1921 cagggagaca gactgcctga ttttcctttg aacttggaag atttggacaa agaagtgcgc
     1981 cgttatgcgg aggctgcggc gagacgttcg ggcggcaggc gcgacctttc tcactgaacg
     2041 caaatgccgt ctgaaaccgg ttttcagacg gcattggaga gtcaaacgct ttttgcaata
     2101 ccgtatatgc cgaatccgat tcgctacaaa ttgaaaacca caatccaaaa aacggaaata
     2161 cacattaaac gggccttttt accgaccgta tcatcccata ggttttccac accttttttc
     2221 atccataaat tcaaaccatt taagtttgta tccttaccga caattgcagc agcatcatcc
     2281 gcataacggt cacccaaatt agccaaataa cgccaaccgc cataggtatc gccacggaaa
     2341 gcataaccac gcaaggtttg gatttgttct ggtgttaaaa caacttcacc ctcattcata
     2401 ataagatcct ttaaattatt ttttacttga attgtttaca aaactctgta ccaattgctc
     2461 tacttgttgg tgataaccta ccaactcctg ctgtgtgagc tgctgctttt cccgaataaa
     2521 cgggtaatac tgtaccgtga aaccgttaat acaggaacct acgctagtgc tataattatc
     2581 ttcttcagcc tggtttactc gattattcca atgagtaata atcgccggat tttctccttt
     2641 tttaatatag taaaattcat gaaaatatgg attttcagga atatgtcttt tatcttcata
     2701 agcttcatat tcaggatagg cagatggcat ttttttaacc ccgtaagtaa gcaatctttc
     2761 tcccatagtc gtacttaaac tacacgcatc ttttcggata acaatatcgt ccacgctatc
     2821 caaaccatcg cgtagaaatt ggataaagtg tgctttgttt tgctggttgt atggctcaag
     2881 aacccagcct tggtcggttt ttttgtaata gacgctaatc tctatcaaat taatggagta
     2941 agcatcctgc ccgggcgtgt cggaaagcca ttttgtggca ttgtcttgcg tataccgtgc
     3001 aagatagcca taagggataa ccattttggt gccctgaaaa tcaaatgtaa ccgtatgttc
     3061 aaatcctgtc attggctcgg gattgttgaa actggtttgt tcattaacag gttcacatga
     3121 ggtcatagta aaaacactcc caataaacaa aataaaaagt tgataaatgg tagtagcata
     3181 tggtctcata atttcaagcc tagaaattag ttaaagaata tattatggta gatactaact
     3241 ctttaagttg acatattgtg cgatgtcgag ggctgtacca tattttccca tcgtattttc
     3301 ccgttcttta tatgagcggc aggcatcctc ataaaagaag ggataccgat acggaaaaat
     3361 accccggcat aaaaaatata ctgattaatc aatacaaatc caacaaacag ttcatcgtat
     3421 tcttttctgt tggcgtataa aaaatactga tatatgcctt cactttcata gggcgacgct
     3481 ttatgtaccg tcctgtgtgt tgaaacgtcg gttttcctta ccgcctgccc cgcttttccc
     3541 tgattgctgc caactgccgt ccgaacctga accgtcaggt ttcagacggc attttttccg
     3601 cctgttccca caatttccgt ttttgtgcgg cggtcagttt tttgacggcg atttcctgtt
     3661 tgagcgccgt gcctttatcc atcccgcctg caacgatacg catcgccacc ggtttgaata
     3721 cgcgggtata tttcgcgcct ttaccggttg tgtgggcggc aagccgctgt tgcggattcg
     3781 ggctgatgcc gcaatagaac gcgctgtttt cacacaatat caggtaaacg ctccagtttg
     3841 acgcgttcat ctgcctgcat cctgagtttt ttccccgttc caaggcgcaa ctttcagcag
     3901 cagcagcata cccggcaggg caagtatgaa acacagctgg aaaaacggta cataaccgag
     3961 ccattcgatc agataaccgg caaaggaatt gatgaccgtg cgcgggacgg cggacaggct
     4021 ggtaaacagc gcaagctgcg ttgcggtaaa tgcgggattg gtttcacgcg ccatatacga
     4081 tacgaacgcc gccgtcccca accccacgcc gaccgcttcc gcgccgataa ctgccgccag
     4141 catcagcctc tcgcctgtgc cgaccgtgtc gaaaggtccg aaccctgcca gccatacaaa
     4201 ccccaaaacg gttacagcct gcaccgcgcc gaatagccac aaggctttgt ttacgccgat
     4261 tttcagcatc cacacgccgc ccaagatacc tgccgccact gccggccaca gtcctgcatt
     4321 ttttgcaatc aaaccgatgt ctgtcttact gaaacccata tccagataaa acggcgttgc
     4381 caacgcggtt gccatactgt cgccgagttt gtaaaggaag ataaacagca gcacgcacac
     4441 cgccgaagcg atgcccttgc gcgtaaaaaa ttctttaaac ggctctacca cggtctgctt
     4501 caacgtttta ggaacggcag gcggcaacac gggttcgcgc gcaagaaaca gcgtcatcag
     4561 aagaccgggc agcataaaca gcgaagtgat gacaaacacc tccggccacg gcatcctgtc
     4621 tgccagcacc aaactcaatg aaccgggaat cagggcggca acccgatagg cgttcacatg
     4681 aaccgagttg cccaaaccca attcctcatc cgacaaaatc tcgcgcctga acgcatccaa
     4741 tacaatgtcc tgactggcgg aaaaaaaagc gacaagcacc gacaagccgg caatcagcgg
     4801 cagatgatta cgagggttta aaaaggcata agccgccaaa gccgccagca aacctgcctg
     4861 cgtcagcagc atccacccgc gccgccgccc caaaacgggc agcctgaccg cgtccatcag
     4921 cggcgaccac aaaaatttcc aagtaaacgg cagaccgatt aacgccatca gcccgatgct
     4981 cttcaaatcc acctgctcgc tgcgtaacca cgccggaatc aggttaatca gaaagtacag
     5041 cggcagcccc gaggcaaacc ccgtaaaaat acagacaagc atattgcggg aaaagatttg
     5101 cccgataaaa cctgatttcg atgcagtcat cgttcgtgca gcccaaaaaa aacaaaagcc
     5161 tattgtagca aatatcgccc gcctgctgaa aaacgccgcc ccgcgccgcc ggtttgccgt
     5221 tcaaatgcaa aatgccgtct gaaactgtgt tcagacggca ttgcttttct gaaaaccaga
     5281 cgtgcttaca ggctgtaata catttcaaat tccagcgggt gcggcgccat gcggatgcgg
     5341 cgtacgtctt cttctttgaa agcgatgtag ctgtcgatcc agtctttgct gaacacgccg
     5401 ccgcgcagga ggaattcgtg gtcggctttg agggcggcca gtgcttcttc caaagaagcg
     5461 caaacggtcg gcaccaatgc atcttcttcc ggcggcagat cgtacaggtt tttatcggca
     5521 gggtcgcccg gatggatttt gttttgaatg ccgtccaaac ccgccatcaa cagggcggca
     5581 aatgccaaat acgggttggc ggtcggatcg gggaaacgcg cttcgatgcg gcgcgccttg
     5641 ctgctgttca cggacggaat gcggatggaa gcggaacggt ttttggcgga gtatgccagt
     5701 ttggtcggcg cttcaaagtg cggcacgagg cgtttgtagg agttggtgga cggattggta
     5761 atcgcgttca aggctttggc gtgtttgatg atgccgccga tgtagtagag cgcggtgtcg
     5821 ctcaagccgg catagccgtc gcctgcgaac aggttttgac cgtctttcca aatggattgg
     5881 tgaacgtgca taccgctgcc gttgtcgccc ataatgggtt tgggcatgaa agtggcggtt
     5941 ttgccgaagt tgtgggcaac gttttgaatc acatatttca tgtcttgggt ttggtcggcg
     6001 cgtttgacca aagtagcaaa gcgcgtgccg atttccattt ggctgccggt gccgacttcg
     6061 ctgtggtgca cttccacttc aataccgagt tcttccaaaa tgtttaccat cgccgaacgc
     6121 aaatcctgac cgcagtcaat cggtgcaaca ggtgcgtaac cgcctttgac ggtcgggcgg
     6181 tggccggtgt tttgaccgtc catatgcaga ccgcttgccc acgcgccgct ttcggacgtg
     6241 atttcgtaac gggttttgtg catatcggtt tcaaattcta tgccgtcgaa tacgaaaaac
     6301 tcgggttcgg gaccgaaata ggcggtctcg ccgataccgg aagatttcaa ataggcttcg
     6361 gctcggcggg cgatggagcg cgggtcgcgg tcgtaaccct gaccgtcggc gggatcgata
     6421 acgtcgcaag tcaacacaac agtcgcatca tcataaaaag gatcgacgaa ggctgtagac
     6481 gcatcgggac gcaactgcat atcggaagcc tgaatgcctt tccagccgcc gatagacgaa
     6541 ccgtcaaacg cctgaccgtt ttcgaaccac tcttcggggt cttccaacac gatgcgcgca
     6601 ggcacggtaa agtggtgctg cttgcctttg gtatcggtaa agcgcaaatc gacaaagcgg
     6661 gcttcgcttt cttcaatcaa ttttacggcg tttttaatag acatttttca gctcctggaa
     6721 aagggaaata gcaaaatata cggaacgcgg cagattagcc caaagccagc atgattctac
     6781 cacgtccatg ccgtctgaaa ctcgccctgc gtcaaatttc aagctggaaa atatagtttc
     6841 agacggcaag attgtcaaca atatgcttcg gtttgtctgt tcaaaagcag aatacccgcc
     6901 cttttgtccg aatgccgaca aaacagatg
//
LOCUS       DAXHTR010000113         6892 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.113, whole genome shotgun sequence.
ACCESSION   DAXHTR010000113 DAXHTR010000000
VERSION     DAXHTR010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.113"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..848)
                     /gene="mafB"
                     /locus_tag="ACFNML_001601"
     CDS             complement(<1..848)
                     /gene="mafB"
                     /locus_tag="ACFNML_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HFC6392952.1"
                     /translation="MNLPIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENAGAASGFFSRADEAGKLIWESDPNKNW
                     WGNRMDDIRGIIQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAA"
     gene            complement(888..1850)
                     /gene="mafA"
                     /locus_tag="ACFNML_001602"
     CDS             complement(888..1850)
                     /gene="mafA"
                     /locus_tag="ACFNML_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HFC6392953.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPARSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVETDNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(2114..2263)
                     /locus_tag="ACFNML_001603"
                     /pseudo
     CDS             complement(2114..2263)
                     /locus_tag="ACFNML_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2489..3208)
                     /gene="pyrH"
                     /locus_tag="ACFNML_001604"
     CDS             complement(2489..3208)
                     /gene="pyrH"
                     /locus_tag="ACFNML_001604"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFC6392954.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDPSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(3452..4306)
                     /gene="tsf"
                     /locus_tag="ACFNML_001605"
     CDS             complement(3452..4306)
                     /gene="tsf"
                     /locus_tag="ACFNML_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFC6392955.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPATVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKKAVDYAA
                     EVAAAAKV"
     gene            complement(4435..5163)
                     /gene="rpsB"
                     /locus_tag="ACFNML_001606"
     CDS             complement(4435..5163)
                     /gene="rpsB"
                     /locus_tag="ACFNML_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFC6392956.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQEAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAEGGFNKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(5336..5569)
                     /locus_tag="ACFNML_001607"
     CDS             complement(5336..5569)
                     /locus_tag="ACFNML_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392957.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFT
                     ITVRRRNHLMRQQEQAESEQQRAQRQKDSGTKP"
     gene            5647..6228
                     /locus_tag="ACFNML_001608"
     CDS             5647..6228
                     /locus_tag="ACFNML_001608"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFC6392958.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGVKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     AKLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVGTLPREAHDLPLDGFVSEAGILCF"
     gene            6289..6879
                     /locus_tag="ACFNML_001609"
     CDS             6289..6879
                     /locus_tag="ACFNML_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HFC6392959.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
BASE COUNT         1553 a         1583 c         1886 g         1870 t
ORIGIN      
        1 gccgcaagtt gtgcttccgg acttaaattt cctaaattgt gaataccctg taaagttttc
       61 cgtgctgccg cataggttac cgggtttacc gcactgtctg taattgcccc aactcccaat
      121 ccctgaaaac ccgttagaaa aggattggct gcaccttgga tgatgccgcg aatatcatcc
      181 atacggttac cccaccaatt tttattgggg tcgctttccc atatcagttt tcctgcttca
      241 tccgcacggc tgaaaaaacc agaggcggca ccggcatttt cttttagcca acggtctgaa
      301 aatggggctt gagggacaat attagtcttt gtttttgttg aagttccttt gacatagtag
      361 ctgtatatat cccttgctcc tccggggggc ggataatcgc tgccttgcgg tccgtcatat
      421 ccatcctccg gatggatttc cgaccctgtc cgatgaagtt ggtaaacagt aaaaccgccg
      481 tctacaccgc cgctgaaatc agaagtgctt tttgaatcat gatgatcgaa cggactgtgt
      541 acttcatgtc cgtgccctga aaaatgggtt tcatagccga taacaccttc aaaccctgtc
      601 cgttcgtgtg taataggcag tatggggccg accgcagttg catcaaatgt ttggacggcg
      661 taaacccgat ttttaacact gccgcgacca tcaccgaaca gatggtattt gccacccggt
      721 tcgtagtgtt ttgcctgcat atcatcgcgc aaacgggcat ccaaaccgtt cgcatgacta
      781 atggggattt gcagcaacga tattgctgct gcaaacagca tcatgaattt ttgaataggc
      841 aaattcattt tgttttcctt ggtagcaagc ggttatggta gtgtgaatca aggttgccct
      901 tgtctatgtt gtcgcactac ttcatcgctg tatccatacc cctcatgact gttatcagtc
      961 tctacggatg gggcggagtt acccgtatga ttgccgtatg gtcggatatc ggagaaatcg
     1021 accattaatc cttccgtcgg tttaattcct ttgcttactt tatacggccc catccacaat
     1081 gcgtaatttt ctttataggc agcttcaaac gcattggttt tgggtttgat gagcaatttt
     1141 ttattggttc tgtctactgc gaaatattcc agttttgttt gggctttcag tgtttcggca
     1201 ttgtataggt gcatttcggt tctgttgcgt atcgttccga atacgtcgat gttaataaac
     1261 acatctgtat cggcattggc aggagaaaca acgtctatgc cgcgcaggaa aaatacggtc
     1321 tgtaccaagt gggaaagaaa ggcagtgtcg cgcgggttag tcgtcaaggt ttcatttcga
     1381 taatccccca tcccgccaat atttaagccc agactgcttt tacttccgct accgtctgat
     1441 tgggtgcgag agcgtgcagg ggcattaagt gtagataaag aagtggttaa acctgtcaaa
     1501 ccgcctgatg ttgtttcagc ggtggtttcg taacgtggat aggtgtaatc ggtacggacg
     1561 gcagggctgt ttatgtattc gccacgaatc agtgcatcaa tggagtagcg accccctgtc
     1621 aaactgcctg aaccttggtc gcccatagtt gcaatgtaca atgcaacttt tcgtccgtgt
     1681 aatgcctgta aatccatgtc tttaacggca gctctggcag aagcggccac aagttcttgt
     1741 tcgaccgcaa agcgtttacc tccgccatgc gatggaatac ctgtcagtgt cccgcaggcg
     1801 gataaaataa aaactgaaaa aagaataggt atcagcagcc gtgcttgcat agattttctc
     1861 ctttgatgaa aaacaaattg tatcaaaatt gtaaacaaat tataaattaa ataaaatatt
     1921 tctttactgg ttttgtggtt tggttttcaa taggctgccc gcacatagtg ggatttacgg
     1981 ttgcaatggc tgcggcgtat tttatagtgg attaaatttt aatcaggaca aggcgacgaa
     2041 gccgcagaca gtacaaatag tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa
     2101 tccactatag gcttttttcg gtcagttgcc ggttaatcag tcccggcagt tcggacaggg
     2161 tgttgtcttg cgccaacctg ttgcggtagc ggcataaggt gctgtaatcg gggatgcttg
     2221 gtttgtcaaa acggcaaaac aggttgaaat cggtgcgggt gatgggtacg gcgttgccct
     2281 tatgccgatt ttgtccgcct gctgcaaggc ggtttgtcta atgaaaggaa aaatgccgtc
     2341 tgaacattgt tcagacggca ttttcaaatg gaaggcaata tagtcggcat atgaaaaatt
     2401 gatgtataag gcaggatggg tttaggcttg ttaatttcca tataataaat ggccgtatga
     2461 agcccatatg cgactatatc tgccggaatc agcagtgaac cagtgtaccc tcgttttcgc
     2521 cggttaccac gcgtttgagc gagccttctt tagcgatacc gaatactacg atattgagct
     2581 tgcgttcgcg gcagagggcg aaagcggtgg catccatcac tttaaggttt ttcaacaagg
     2641 cttcgtcaaa agtaatggtt tcgtagcgtg ttgcagatgg gtcttttttc gggtctgcgg
     2701 tgtacacacc gtcaacgttg gtggctttga gcatcacgtc gcagttcatt tctgcaccgc
     2761 gcaatgcggc ggcagtgtcg gtcgtgaaga acggattacc ggtaccggcg gcgaaaatca
     2821 cgactttgcc ttcttccaaa tattgaatgg ctttaggacg ggcgtaagtt tcggcgattt
     2881 gctgcataga cagtgcggat tgtacgcgcg ctttgatgcc caaagtttca aatgcgtctt
     2941 tgagtgccaa cgcgttcatt acggttgcca tcatgcccat gtagtcggcg gtggcgcggt
     3001 ccatactgcc tgcttgtgca gatacgccac ggaaaatgtt accgccgccg acaacgatac
     3061 cgacctgcac gcccatttta acgacttcgg caatttcgcc gacagtttga acgatggtat
     3121 cgtgattgat gccgaacgga tcggaaccca tcagggattc gccggagagt ttcagtaata
     3181 cgcgtttgta tttgatttgc tgtgtcatgg gatattctgc tttctcgttg gggatgttgc
     3241 cggcttccgg gcgggatgcc gtctgaaagg tgtatgcctt ggcagggcgt atgcggcgtg
     3301 ccggcggtgt gaaaacgggc tgctgaattt gccgccgctg ttttcgggcg gggcgggcat
     3361 ttgaaccctg ccgccggaaa atatgggcct ttcccaaaaa aaagcaccct gattcgtttg
     3421 gaatccaggt gctttctttt tcataagtgc cttacacttt ggcggcagcg gctacttctg
     3481 ccgcgtaatc gacggctttt ttctcgatac cgtcgcctac tttgtagcgg acgaagctga
     3541 ccacttcagt gccgttttct ttagcgaatt gggcaacagt ttggtcgggg ttcatcacga
     3601 acgcttggcc gttcagagtg atttcagcca agaatttgcg gatacggcct tcaaccattt
     3661 tagcggcgat gtcggcaggt ttgccggaag cgatagcttg ttcagtgtag atgtggcgtt
     3721 ctttttcaac ggtttcagca tctacttcgg cttcacttac gcattgtggt ttagcggcaa
     3781 cgatgtgcat accgattttg cgtgctacgt cttcagagcc tttgtactca accaatacgc
     3841 cttcggtagc caaagcaccg tggatgtagg caaccagttg gttggcagtg tcgatcactt
     3901 ggaagcggcg aacagacatg ttctcgccca atttagcgat gatggcttta cgttctgctt
     3961 caaccagttc gctcagttct tcaacggtag ccggtttttt ctcggcagcg gttttagcaa
     4021 cgaagttggc aaattctacg aagcccgcgt ctttggcaac gaagtcggtt tcgcagttta
     4081 cttcgaccaa tgcgccgaca ttgccgttga tggcgtaagc taatacgcct tcggcagcgg
     4141 tacgaccggc cagtttaccg gctttcgcgc cggatttgat gcgcaggatt tcttcggctt
     4201 tgtcgaagtt gccttcagct tcaaccaagg cttttttgca ttccatcatg cccaagccgg
     4261 tagcggcgcg cagatcggca accatttttg cagtaatttc tgccattttg aatctcctag
     4321 atttttggga aatacggaaa ggattttccg cattcgggaa tcgtgtgccg cgaacggacg
     4381 ttcagacggc atatttgaga aaaggggcat aacgcccctc ttctgtacac cgaattactc
     4441 ggcagcagct tcttgggcag cggctacagt ttcttgcagc gcttggtttt tgccttccaa
     4501 aactgcgtcg gcgatgccgc ggcagtacag gcggatggct ttggcggagt cgtcgttgcc
     4561 ggggataacg tatttcacgc cgtcagggct gttgttggta tcgactacgg cgataacggg
     4621 gatgcccaat ttctcagctt cgaccagagt acctttttgg tagccggtat cgataacgaa
     4681 aatcgcgtca ggcaggcctt tcatgttttt gataccgccc aaagaacgtt ccagtttttc
     4741 aacgtcgcgt tgcatttcca gaatttcttt tttgttgaaa ccgccttcag cagcgttttc
     4801 cagggcagca gttttttctt ccaggcgttt gatggattgc ttaacggttt tgtagttggt
     4861 cagcatacca cccaaccagc ggtaatcaac gaaaggcata ccggcacggg tagcttcttc
     4921 gcggatgatg tcgcgggctt ggcgtttggt acctacgaac aatactgtac ctttgttggc
     4981 aaccagacga cgtacggctt cttgcgcctc ttggaacatc ggcagggttt tttccaagtt
     5041 tacgatatgg attttgttgc gcgcaccgaa aatgtattgt gccattttcg ggttccaaaa
     5101 acgggtttgg tggccgaagt gaacaccggc ttcaatcatc tgacgcatag taatttgaga
     5161 cattttattt ccttgaaagg gttaagagca cacattcaat cgcatagaac ttgataggtc
     5221 acgcctattg tcaagcaccc ttcggcgaaa gtgggcataa gttttaaaaa atagtgtgtt
     5281 tccgaaatgg aaaactgacg gattatagcg gataagatgc cgtctgaaaa gggattcagg
     5341 gttttgtgcc gctgtctttt tgccgttgtg cgcgctgctg ttcggattcc gcctgttcct
     5401 gctgcctcat caggtggttg cggcggcgga cggtaatggt gaaaaccgtg aacacggtgg
     5461 gcagcaccgc ccaaaaaacc aaataaatca aggcgcgcgc aatactcggc tgcgcggcgg
     5521 aatacataac ggcaacaaaa agatagccga tggcgacgat gtgccacatt gtgtgcgtcc
     5581 tgtttgaatg ttaacaaaat cgttataatc gcaatattct accgcaatcc tgaaaggccg
     5641 tctgaaatga ggaacgagga aaaacgcgcc ctgcgccgcg aattgcgcgg gcggcgttcg
     5701 caaatggggc gggacgtgcg ggcggcggca acggtaaaaa tcaaccacct gctcaaacgt
     5761 tatattaaaa aagggcggaa aatcggcgtg tattggccga tgggcaagga attgcgtttg
     5821 gacggctttg tccgcgcggc gcaaaaacgc ggtgtaaaac tttatctgcc ttatatcgaa
     5881 ccgcgttcgc ggcggatgtg gtttacgccg tatcctgaaa gcggaatgga acgggagcgc
     5941 atacggggca gggcgaagtt gaacgtgccg cagtttgcag ggcgcaaaat ccgcgtgcac
     6001 ggtttgtcgg tattgctcgt cccgcttgtc ggcatagacc gcgaaggcta ccgcttgggg
     6061 caggcaggcg gctattacga tgcgacgctt gcagcgatga aataccgttt gcaggcaaaa
     6121 accgtgggcg tgggctttgc ctgccaactg gtcggcacgc tgccgcgcga ggcgcacgac
     6181 ctgccgctgg acggttttgt gtcggaggcg gggatattgt gtttttagcc gttcagacgg
     6241 catttcgggc ggaaatgccg tctgaaggcg gaatattggg agaaaaggat gtcggaaaag
     6301 ataatactgc cggtcttgtc attgggcggc gttcggctcg aaccgttgga tgtgcatcat
     6361 gaaacgggtt tgcgcgaggc ggtttgcgat ggggaggttt ggaagctggg ggtaacgtcc
     6421 gcgccccatc cggatagggt ggcagactat atcgggacag cattggcaac gcgtttggcg
     6481 tttgcggttg tcgatgaaga ggcgggcagg gtggtcggga caacggcgta ttatcatttt
     6541 gaaccgcaga ttccgcgttt ggatatcgga ttcacatggt atgcggcatc ggcacggcgg
     6601 acacggataa acacctgttg caaaatcatg ctgttggatt acgcatttga tgttttggct
     6661 tgccgttgcg tgggatggcg cacggatatt ctgaaccttg cctcgcaacg tgccatcgaa
     6721 cggctgggtg cggaaaaaga cggcgtgctg cgtatgcata tgctccgtaa agacggcagt
     6781 gtgcgcgata cggtcgtgta cagtatgctg agggaagatt ggtgcaaaaa cagggaaatc
     6841 ctgaccggca ggttggcagg gtatggcgtg caggtgtgag gcagtatttt ca
//
LOCUS       DAXHTR010000114         6709 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.114, whole genome shotgun sequence.
ACCESSION   DAXHTR010000114 DAXHTR010000000
VERSION     DAXHTR010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6709
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.114"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            127..1512
                     /locus_tag="ACFNML_001610"
     CDS             127..1512
                     /locus_tag="ACFNML_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HFC6392960.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            1532..2050
                     /locus_tag="ACFNML_001611"
     CDS             1532..2050
                     /locus_tag="ACFNML_001611"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HFC6392961.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(2389..3306)
                     /gene="thrB"
                     /locus_tag="ACFNML_001612"
     CDS             complement(2389..3306)
                     /gene="thrB"
                     /locus_tag="ACFNML_001612"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFC6392962.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACTRLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWARTADNKLDEALKKVFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLD"
     gene            complement(3343..4071)
                     /gene="ubiG"
                     /locus_tag="ACFNML_001613"
     CDS             complement(3343..4071)
                     /gene="ubiG"
                     /locus_tag="ACFNML_001613"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFC6392963.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLCGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACAKLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            4223..5464
                     /locus_tag="ACFNML_001614"
     CDS             4223..5464
                     /locus_tag="ACFNML_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter"
                     /protein_id="HFC6392964.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRGWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSALGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKMPVLFDTQAPVGTNYWIYAATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRQKFGAGKTYKVYGGLWLMVWVFLF
                     GIINIAAQVLSQMELVPVFKG"
     gene            5614..6669
                     /locus_tag="ACFNML_001615"
     CDS             5614..6669
                     /locus_tag="ACFNML_001615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6392965.1"
                     /translation="MKLKIDIATNNFKHGGGTERYTLDLVKGLNRQNITPAVYATKFD
                     HGIPEYAMIEPHLVDQHRTLKKLRSFLFSSRLAQTRKNSAAKLIACHHADYADLLICG
                     GTHLGYLHHMAQKPNLLDRLAIRRNRSNYATAKLIMAHSHMMQRELVGLYGVPPERIQ
                     VAPPPADTERFFPQPRETAALRAKYGFADHETVFLFPSTGHTRKGLELLADFFEHTDL
                     PVKLAVVGSPLPRPMKNVVGLGFCTDMPELYRAADFTIMASLYEPFGLVGVESVLCGT
                     RVVLSENMACTEVMNEEAGFFFSRQNPETLAQAVAQAVSLKKQGGHRLSDPMRALNYN
                     PSLSHHIDRLTDILASV"
BASE COUNT         1532 a         2121 c         1618 g         1438 t
ORIGIN      
        1 atcgttcaaa taaacaggaa tataacttca gacaaacaac ttaccgcccg atttgtgcta
       61 tcgttttcgc acaacttaaa aaaacctgac aattttgtac ttttattaca gagaaaggct
      121 ttacaaatgg acggctggac acagacgctg tccgcgcaaa ccctgttggg catttcggcg
      181 gcggcaatca tcctcattct gattttaatc gtcaaattcc gcatccacgc gctgctgaca
      241 ctggtcatcg tcagcctgct gacggctttg gcaaccggtt tgcccacagg cagcattgtc
      301 aacgacgtac tggtcaaaaa cttcggcggc acgctcggcg gcgtggcgct tctggtcggc
      361 ctgggcgcga tgctcggacg tttggtcgaa acatccggcg gcgcacagtc gctggcggac
      421 gcgctgatcc ggatgttcgg cgaaaaacgc gcaccgttcg cgctgggcgt tgcctcgctg
      481 attttcggct tcccgatttt cttcgatgcc ggactaatcg tcatgctgcc catcgtgttc
      541 gccaccgcac ggcgcatgaa acaggacgta ctgcccttcg cgcttgcctc catcggcgca
      601 ttttccgtca tgcacgtctt cctgccgccc catccgggcc cgattgccgc ttccgaattt
      661 tacggcgcga acatcggcca agttttgatt ttgggtctgc cgaccgcctt catcacatgg
      721 tatttcagcg gctatatgct cggcaaagtg ttggggcgca ccatccatgt tcccgttccc
      781 gaactgctca gcggcggcac gcaagacaac gacctgccga aagaacctgc caaagcagga
      841 acggtcgtcg ccatcatgct gattcccatg ctgctgattt tcctgaatac cggcgtatcg
      901 gccctcatca gcgaaaaact cgtaagtgcg gacgaaacct gggttcagac ggcaaaaata
      961 atcggttcga caccgatcgc ccttctgatt tccgtattgg tcgcactgtt tgtcttggga
     1021 cgcaaacgcg gcgaaagcgg cagcgcgttg gaaaaaaccg tggacggcgc actcgccccc
     1081 gtctgttccg tgattctgat taccggcgcg ggcggtatgt tcggcggcgt tttgcgcgct
     1141 tccggcatcg gcaaggcact cgccgacagc atggcggatt tgggcattcc cgtccttttg
     1201 ggctgtttcc ttgtcgcctt ggcactgcgt atcgcgcaag gttcggcaac cgtcgccctg
     1261 accaccgccg ccgcgctgat ggctcctgcc gttgccgccg ccggctttac cgactggcag
     1321 ctcgcctgta tcgtattggc aacggcggca ggttcggtcg gttgcagcca cttcaacgac
     1381 tccggcttct ggctggtcgg ccgcctcttg gacatggacg taccgaccac gctgaaaacc
     1441 tggacggtca accaaaccct catcgcactc atcggctttg ccttgtccgc actgctgttc
     1501 gccatcgtct gacagacgga aaggatagta aatgactacg cattttgtcg ttatgggcgt
     1561 atgcggctgc ggcaagacca ccgccgcgct gtccctgcag aaacacctcg gtcaatgtcc
     1621 ctatgccgaa ggcgacgagt tccacaccca agccaaccgc gacaagatgg gcgcgggtat
     1681 tccgctgacc gatgaagacc gctatccgtg gttgggcaat ctgcgcgact ggatgacgca
     1741 acaggcgcaa aacggtgcga accacaccat cgtaacctgt tccgccctca aacgcggcta
     1801 ccgcgacatt ctgcgcggag ccgaaggcaa agctgccttc atccacctca gtccgccgca
     1861 agacatcaac ctcgagcgca tgatgtcgcg caaaggacat tacatgaaag cagggatgct
     1921 cgattcgcaa ctggaaatcc tcgaggaact gggcgaaggc gaatacgggg tcaaaatcgc
     1981 caaccccggc acgcccgaag cggtcgaagc cgatattctg aactgggttg cctcggaaaa
     2041 cctgctttga agcaatatgc cgtctgaagc ccgacacagg atgggtttca gacggcataa
     2101 acatcgggaa cagaatggat tacattgatt tatagtggat taacaaaaac cagtacagcg
     2161 ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc gagtgaatcg
     2221 gttccgtact atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
     2281 ctataaaatg gaaaataccc ggctatcgtc tcattttcgt tttaatcagc cataaaaatg
     2341 ccgtctgaaa ccctttcaga cggcatttct gtcaaacgcc ggacgcactc aatccaaact
     2401 caacagcagg ttgcggaacg cgttcgggtc tttgataaac gtcatctcgc ccgcctgcgg
     2461 aaaatgaaag tccaacaggc gcgacaccca aaaacggatg cagccggcac gttgggcggt
     2521 cgggaaatac gccttttctt cggcactcaa ggggcgcacg ccctcataac cgccgataaa
     2581 cacctttttc aacgcctcat ccaacttatt gtccgccgtc cttgcccaat cgttgaccgc
     2641 aatcgccaag tcatacataa aattgccccg gcaggcataa tagaaatcga tgaaacccga
     2701 tacctgaccg ccgtcaagca acacattgtc tttaaacaaa tcggcatgga tgatgcccga
     2761 aggcagatga ttgccgagat tgtcctttaa cgcatcgatt tcggaacaca gcagcgcggc
     2821 atcgtcttgc gacaggacgg gcagcagccg ggtgcacgcc tccgtccacc acgcatcgta
     2881 acgcgggttt tccatttcca aagggaaatc ggcggcggca aggtgcattt tggccagcat
     2941 cgcaccggta tggaaacact gctccgccgt cggcagcgcg gtatccgaac ctttcaggca
     3001 ggcaaccaaa caagccggct tgcccgccaa aacggaatca agccggccgt ctttgcgcgc
     3061 aaccggcgcg gcaaccgcca cgcccttcat actcaaatgc cggttaagct ccagaaaaaa
     3121 cggcagctct tcctgtttca acacttcaaa cacggtcagc acataacgtc ccgaagtcgt
     3181 cgtcagaaaa taattgctgt tggtaatccc ctgcgcgatg ccctgcaggg aaacaaattc
     3241 ccccaaatcg taaccgctca ggaagccgcg catttcatca tcggaaacac tggtatagac
     3301 agacatatca actcacttgc tcaaaaacgg gcaaacccgc cgtcagaacg ccggacggca
     3361 ggcaaacata taattcacat cggtcgaatc gcacagggca taagtttgcg acaacacatg
     3421 gtaagtcata cccttcgtat ccgccacatc caagcctgcc tgacggcaca ttcgcgccag
     3481 ctcggcaggt gcgatgaatt ttttccagtc gtgcgtgcct ttggggacaa acttcaacag
     3541 atattccgcc gccacaatca gatgcaggta cgatttcggg tttttattga tggtggaaaa
     3601 aaacaccatg ccgtccggct tgaccagctt ggcacaagca cgcacgatgg cggcgggatc
     3661 ggggacgtgt tccatcattt ccatgcacgt taccacatcg aacgagtgcg gttccgcctc
     3721 ggcaaggtct tccacgcgga tacattcgta ttcgatatcg gcgacattgt tcaaagccgc
     3781 gtgcaggcgg gcggtttcca acgactgctc cgccatgtcg atgcccttta caaacgccgc
     3841 gccgcgccgt gccatacttt ccgccaagat gccgccgccg cagcccacgt ccaaaacccg
     3901 tttgccgcac aaatccgcgt gcccgtcgat ataatccagc cgcagcggat tgatgtcgtg
     3961 caaggttttg aactcgcccg acttgtccca ccatttgtcg gcaatccggc tgaatttggc
     4021 gatttccccc tcatcgacat tatatttttt gtcggacatt ttccctccca tctgacgaac
     4081 cgcccactcc aaaacccaag attatactat ggaacacaat gccgtctgaa agccttttcg
     4141 ggcggcgcgg cgcaaatgcc ttacaaatcc ttacacttta cggcataatg gcggctcgct
     4201 ttttctggca gaaagacaaa atatgcccac caaaacccct tcactgttcg gcggcgcgat
     4261 gattatcgcc ggcacggtca tcggcgcagg tatgctcgcc aacccgaccg ccacatccgg
     4321 cgtatggttt accggctcgc tggccgtgtt gctgtacacc tggttttcta tgctttccag
     4381 cggcctgatg attttggaag tcaacaccca ctatccccac ggcgcaagtt tcgacacgat
     4441 ggtcaaagac ctgctcggac gcggctggaa catcatcaac ggcatcgccg tcgccttcgt
     4501 tttatacctg ctcacctacg cctacatctt cgttggcggc gacctgaccg ccaaaggctt
     4561 aggcagcgcc ttgggcggca atgtttcact caccgtcgga caactcgtct tcttcggcat
     4621 cctcgccttt tgcgtatggg catccgcacg cttggtcgac cgcttcacca gcgtcctcat
     4681 cggcggcatg gtattaacct ttatttgggc aaccggcggc ctgattgccg atgccaaaat
     4741 gcccgtcctg ttcgacaccc aagcccccgt cggcaccaac tactggattt acgccgccac
     4801 cgccctgccc gtctgccttg cgtcattcgg tttccacggc aacgtctcca gcctgctcaa
     4861 atactttaaa ggcgacgcgc ccaaagtggc taaatccatc tggacgggca cactgattgc
     4921 gctggtaatt tacgtcctct ggcaaaccgc catccaaggc aacctgccgc gcaacgagtt
     4981 cgcccccgtg attgccgccg aagggcaagt ctccgtcctc atcgaaaccc tgtccaaatt
     5041 cgcccaaacc ggcaatatgg ataaaatatt gtccctgttt tcctatatgg cgatcgccac
     5101 ctcgttttta ggcgtaacct taggtctgtt cgactacatc gccgacatct tcaaatggaa
     5161 cgacagcgtg tccggccgca ccaaaaccgc cgcgctgacc ttcctgccgc ctctaatttc
     5221 ctgcctgctc ttccccaccg gcttcgttac cgccatcggc tacgtcggcc tggcggcaac
     5281 cgtctggaca ggcatcatcc ctgccatgct gctctaccgc tcgcgccaaa aattcggcgc
     5341 aggcaaaacc tataaagttt acggcggctt gtggctgatg gtttgggtct tccttttcgg
     5401 catcatcaac atcgccgcac aggtattgag ccaaatggaa ctcgtccccg tatttaaagg
     5461 ataaaggcaa aatgccgttt gaagcccgcc ggcggcttca gacggcattg ccgcaacaaa
     5521 cggcaaccgt attccggcac acagcgcatt accctgcccc tcacgcacaa atcccgcccc
     5581 gacaaaccgg gacgcaacca taaaggaaca atgatgaagc tcaaaataga tattgcaacc
     5641 aacaacttca aacacggcgg cggcacggaa cgctacacat tggatttggt aaagggtctg
     5701 aacagacaaa acatcacacc ggccgtttat gcgacgaaat ttgatcacgg cattcctgaa
     5761 tacgccatga tcgaacccca tcttgtcgat caacaccgga cgctgaaaaa actacgctca
     5821 ttcctctttt caagccggct cgctcaaacc agaaaaaaca gtgccgccaa actgattgcc
     5881 tgccaccacg ccgattacgc cgacctcctc atctgcggcg gcacacactt gggctacctg
     5941 caccatatgg cgcaaaaacc gaacctgctc gaccgcctcg ccatacgccg caaccgcagc
     6001 aactacgcca ccgccaaact gattatggcg cattcccata tgatgcagcg cgaactggtc
     6061 ggactatacg gcgttccccc cgaaagaatc caagtcgccc ccccccccgc agatacggaa
     6121 cgcttctttc cacaacctag agaaactgcc gctctgcgcg ccaaatacgg ttttgccgac
     6181 catgaaaccg ttttcctatt tccatcgacc ggccacacgc gcaaaggcct ggaactgctt
     6241 gccgactttt tcgaacatac cgacctgccc gtcaagcttg ccgttgtcgg ctccccgctc
     6301 ccccgcccta tgaaaaacgt cgtcggactg ggcttctgca ccgatatgcc cgaactctac
     6361 cgcgccgccg actttaccat tatggcctcc ctgtatgaac ccttcgggct ggtcggtgtc
     6421 gaatccgtcc tatgcggcac acgcgtcgtc ctctccgaaa acatggcatg tacagaggtc
     6481 atgaacgaag aagccggatt ctttttctca cgccaaaacc cggaaaccct agcgcaagcc
     6541 gttgcccaag ccgtcagcct taaaaaacag ggcggacacc gcctgtccga cccgatgcgt
     6601 gcactgaact acaacccgtc cctgtcgcac cacatcgacc gactgaccga catactggca
     6661 tccgtataac aaaatgccgt ctgaacgagt tttcagacgg cattgctgc
//
LOCUS       DAXHTR010000115         6660 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.115, whole genome shotgun sequence.
ACCESSION   DAXHTR010000115 DAXHTR010000000
VERSION     DAXHTR010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.115"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            285..2132
                     /locus_tag="ACFNML_001616"
     CDS             285..2132
                     /locus_tag="ACFNML_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HFC6392966.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAP
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNTLENWQQPVGSDFDQDSWENSKTSVLGAVTRKGYPLAKLGNTRAAVNPDTATVDL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDVDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGTFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNADVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLTGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMELKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            2187..6356
                     /locus_tag="ACFNML_001617"
     CDS             2187..6356
                     /locus_tag="ACFNML_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HFC6392967.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMRRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGSAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADCNGIPVRQVLGGFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTEKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRINPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDRKTGISAKGGARGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     HFGRINADLDIGNAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNVAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTRKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNGKGK"
BASE COUNT         1571 a         2135 c         1748 g         1206 t
ORIGIN      
        1 aaggttgcgt tgatttgccc tatgcagtgc cggacaggct ttgcttgatc attcggcgca
       61 acggtttaat ttattgaacg aaaataaatt tatttaatcc tgcctatttt ccggcactat
      121 tccgaaacgc agcctgtttt ccatatgcgg attagaaaca aaatacctta aaacaagcag
      181 atacatttcc ggcgggccgc aacctccgaa ataccggcgg cagtatgccg tctgaagcgt
      241 cccgccccgt ccgaacagtg ttaaaatcga aatccgccac accgatgcac gacacccgta
      301 ccatgatgat caaaccgacc gccctgctcc tgccggcttt atttttcttt ccgcacgcat
      361 acgcgcctgc cgccgacctt tccgaaaaca aggcggcggg tttcgcattg ttcaaaaaca
      421 aaagccccga caccgaatca gttaaattaa aacccaaatt ccccgtccgc atcgacacgc
      481 aggatagtga aatcaaagat atggtcgaag aacacctgcc gctcatcacg cagcagcagg
      541 aagaagtatt ggacaaggaa cagacgggct tcctcgccga agaagcaccg gacaacgtta
      601 aaaccatgct ccgcagcaaa ggctatttca gcagcaaagt cagcctgacg gaaaaagacg
      661 gagcttatac ggtacacatc acaccgggcc cgcgcaccaa aatcgccaac gtcggtgtcg
      721 ccatcctcgg cgacatcctt tcagacggca acctcgccga atactaccgc aacacgctgg
      781 aaaactggca gcagccggta ggcagtgatt tcgatcagga cagttgggaa aacagcaaaa
      841 cttccgtcct cggcgcggta acgcgcaaag gctacccgct tgccaagctc ggcaacaccc
      901 gggcggccgt caaccccgat accgccaccg tcgatttgaa cgtcgtcgtg gacagcggcc
      961 gccccatcgc cttcggcgac tttgaaatca ccggcacaca gcgttacccc gaacaaatcg
     1021 tctccggcct ggcgcgcttc caaccgggca cgccctacga cctcgacctg ctgctcgact
     1081 tccaacaggc actcgaacaa aacgggcatt attccggcgc gtccgtacaa gccgacttcg
     1141 accgtctcca aggcgaccgc gtccccgtca aagtcagcgt aaccgaggtc aaacgccaca
     1201 agctcgaaac cggcatccgc ctcgattcgg aatacggttt gggcggcaaa atcgcctacg
     1261 actattacaa cctcttcaac aaaggctata tcggctcggt cgtctgggat atggacaaat
     1321 acgaaaccac gcttgccgcc ggcatcagcc agccgcgcaa ctatcggggc aactactgga
     1381 caagcaacgt ttcctacaac cgttcgacca cccaaaacct cgaaaaacgc gccttctccg
     1441 gcggcatctg gtatgtgcgc gaccgcgcgg gcatcgatgc caggctgggg gcagagtttc
     1501 tcgcagaagg ccggaaaatc cccggctcgg atgtcgattt gggcaacagc cacgccacga
     1561 tgctgaccgc ctcttggaaa cgccagctgc tcaacaacgt gctgcacccc gaaaacggcc
     1621 attacctcga cggcaaaatc ggtacgactt tgggcacatt cctgtcctcc accgcgctga
     1681 tccgcacctc tgcccgcgca ggttatttct tcacgcccga aaacaaaaaa ctcggcacgt
     1741 tcatcatacg cggacaagca ggttacaccg ttgcccgcga caatgccgac gttccttcgg
     1801 ggttgatgtt ccgcagcggc ggcgcgtctt ccgtgcgcgg ttacgaactc gacagcatcg
     1861 ggcttgccgg cccgaacgga tcggtcctgc ccgaacgcgc cctcttgacg ggcagcctgg
     1921 aataccaact gccgtttacg cgcacccttt ccggcgcggt attccacgat atgggcgatg
     1981 ccgccgccaa tttcaaacgt atggagctga aacacggttc gggactgggc gtgcgctggt
     2041 tcagcccgct cgcgccgttt tccttcgaca tcgcctacgg gcacagcgat aagaaaatcc
     2101 gctggcacat cagcttggga acacgcttct aaaccgatac ggccgcttca gacggcattg
     2161 cagcaaacta ttttgaaaca gacattatga ccgataccgc accgacagat accgatccga
     2221 ccgaaaacgg cacgcgcaaa atgccgtctg aacaccgccc caccccgccg gcaaaaaaac
     2281 gccgcccgct gctgaagctg tcggcggcac tgctgtctgt cctgattttg gcagtatgtt
     2341 tcctcggctg gctcgccggc acggaagcgg gtttgcgctt cgggctgtac caaatcccgt
     2401 cttggttcgg cgtaaacatt tcctcccaaa acctcaaagg cacgctgctc gacggcttcg
     2461 acggcgacaa ctggtcgata gaaaccgagg gggcagacct taaaatcagc cgcttccgct
     2521 tcgcgtggaa accgtccgaa ctgatgcgcc gcagcctgca cattaccgaa atttccgccg
     2581 gcgacatcgc catcgttacc aaaccgactc cgcctaaaga agaacgcccg ccgctcagcc
     2641 ttcccgacag catagacctg cctgccgccg tctatctcga ccgcttcgag acgggcaaaa
     2701 tcagcatggg caaagccttt gacaaacaaa ccgtctatct cgaacggctg gatgcttcat
     2761 accgttacga ccgcaaagga caccgccttg acctgaaggc cgccgacacg ccgtggagca
     2821 gttcgtcggg gtcagcctcg gtcggcttga aaaaaccgtt tgccctcgat accgccattt
     2881 acaccaaagg cggactcgaa ggcaaaacca tacacagtac ggctcggctg agcggcagcc
     2941 tgaaggatgt gcgcgccgaa ctggcgatcg acggcggcaa tatccgcctc tcgggaaaat
     3001 ccgtcatcca cccgtttgcc gaatcattgg ataaaacatt ggaagaagta ctggtcaaag
     3061 ggttcaacat caatccggcc gccttcgtgc cttccctgcc cgatgccgga ctgaatttcg
     3121 acctgaccgc catcccgtcg ttttcagacg gcatcgcgct ggaaggttcg ctcgatttgg
     3181 aaaacaccaa agccggcttt gccgactgca acggcatccc cgtccgtcag gttttaggcg
     3241 gctttgtcat ccggcaggac ggcacggtgc atatcggcaa tacgtccgcc gccctgctcg
     3301 gacggggcgg catcaggctg tcgggcaaaa tcgacaccga aaaagacatc ctcgatttaa
     3361 atataggcat caactccgtc ggcgcggaag acgtactgca aaccgcgttc aaaggcaggt
     3421 tggacggcag catcggcatc ggtggcacga ccgcctcgcc caaaatctct tggcaactcg
     3481 gcatcggcac ggcgcgcacg gacggcagcc tcgccattgc aagcgacccc gcaaacggac
     3541 agcggaaact ggtgctcgac accgtcaaca tcgccgccgg gcaaggcagc ctgaccgcgc
     3601 aaggctatct cgagctgttt aaagaccgcc tgctcaagct ggacatccgt tcccgcgcat
     3661 tcgacccttc gcgcatcaat ccgcaacttc cggcaggcaa tatcaacggc tcaataaacc
     3721 ttgccggcga actggcaaaa gagaaattca caggcaaaat gcggttttta cccggcacgt
     3781 tcaacggcgt accgattgcc ggcagtgccg acattgttta cgagtcccgc caccttccgc
     3841 gtgccgccgt cgatttgcgg ctggggcgga acatcatcaa aacagacggc ggcttcggca
     3901 aaaaaggcga ccggcttaac ctcaatatca ccgcacccga tttatcccgt ttcggtttcg
     3961 gactcgcggg gtctttaaat gtacgcggac acctttccgg cgatttggac ggcggcatcc
     4021 gaacctttga aaccgacctt tccggcgcgg cgcgcaacct gcacatcggc aaggcggcag
     4081 acatccgttc gctcgatttc accctcaaag gttcgcccga cacaagccgc ccgatacgcg
     4141 ccgacatcaa aggcagccgc ctttcgctgt cgggcggagc ggcggttgtc gataccgccg
     4201 acctgatgct ggacggcacg ggcgtgcagc accgcatccg cacacacgcc gccatgacgc
     4261 tggatggcaa accgttcaaa ttcgatttgg acgcttcagg cggcatcaac agggaactta
     4321 cccgatggaa gggcagcatc ggcatcctcg acatcggcgg cgcattcaac ctcaagctgc
     4381 aaaaccgtat gacgctcgaa gccggtgcgg aacgcgtggc ggcaagtgcg gcaaattggc
     4441 aggcaatggg cggcagcctc aacctgcaac acttttcctg ggacaggaaa accggcatat
     4501 cggcaaaagg cggcgcacgc ggtctgcata tcgccgagtt gcacaatttc ttcaaaccgc
     4561 ccttcgaaca caatctggtt ttaaacggcg actgggatgt cgcctacggg cgcaacgcgc
     4621 gcggctacct caatatcagc cggcaaagcg gcgatgccgt attgcccggc gggcaggctt
     4681 tgggtttgaa cgcattttcc ctgaaaacgc gctttcaaaa cgaccgcatc ggaatcctgc
     4741 ttgacggcgg cgcgcatttc gggcggatta acgccgattt ggacatcggc aacgccttcg
     4801 gcggcaatat ggcaaatgca ccgctcggcg gcaggattac cgcctccctt cccgacttgg
     4861 gcgcattgaa gccctttctg cccgccgccg cgcaaaacat taccggcagc ctgaatgccg
     4921 ccgcgcaaat cggcggacgg gtcggctctc cgtccgtcaa tgtcgccgtc aacggcagca
     4981 gcaactacgg gaaaatcaac ggcaacatca ccgtcgggca aagccgctct ttcgataccg
     5041 cgcctttggg cggcaggctc aacctgaccg ttgccgatgc cgaagtattc cgcaacttcc
     5101 tgcccgtcgg acaaaccgtc aaaggcagcc tgaatgccgc cgtaaccctc ggcggcagca
     5161 tcgccgaccc gcacttgggc ggcagcatca acggcgacaa gctctattac cgcaaccaaa
     5221 cccaaggcat catcttggac aacggttcgc tgcgttcgca tatcgcaggc aggaaatggg
     5281 taatcgacag cctgaaattc cgtcacgaag ggacggcgga actctccggt acggtcggta
     5341 tggaaaacag cggacccgat gtcgatatcg gcgcggtgtt cgacaaatac cgcatcctgt
     5401 cccgccccaa ccgccgcctg acggtttccg gcaacacccg cctgcgctat tcgccgcaaa
     5461 aaggcatatc cgttaccggg atgattaaaa cggatcaggg gctgttcggt tcgcaaaaat
     5521 cctcgatgcc gtccgtcggc gacgatgtcg tcgtattagg cgaagtcaaa aaagaggcgg
     5581 cggcaccgct ccccgtcaat atgaacctga ctttagacct caatgacggc atccgcttcg
     5641 ccggctacgg cgcggacgtt accataggcg gcaaactgac cctgaccgcc caatcgggcg
     5701 gaagcgtgcg gggcgtgggc acggtccgcg tcatcaaagg gcgttataag gcatacgggc
     5761 aggatttgga cattaccaaa ggcacggtct cctttgtcgg cccgctcaac gaccccaacc
     5821 tcaacatccg cgccgaacgc cgcctttccc ccgtcggtgc gggcgtggaa atattgggca
     5881 gcctcaacag cccgcgcatt acgctgacgg caaacgaacc gatgagtgaa aaagacaagc
     5941 tctcttggct catcctcaac cgcgccggca gcggcagcag cggcgacaat gccgccctgt
     6001 ccgcagccgc aggcgcgctg cttgccgggc aaatcaacga ccgcatcggg ctggtggatg
     6061 atttgggctt tacccgcaag cgcagccgca acgcgcaaac cggcgaactc aaccccgccg
     6121 aacaggtgct gaccgtcggc aaacaactga ccggcaaact ctacatcggc tacgaataca
     6181 gcatctccag cgcggaacag tccgtcaaac tgatttaccg gctgacccgc gccatacagg
     6241 cggttgcccg tatcggcagc cgttcgtcgg gcggcgagct gacatacacc atacgtttcg
     6301 accgcttctc cggttcggac aaaaaagact ccgccggaaa cggcaaagga aaataagcgg
     6361 ttttcagacg gcgcgccgcc aaaccggaca tttgaaaacc tgcttttcca ccgtccgccg
     6421 ccgccgtccg cctgcaaggg aacagaatcg atatagtgaa ttaacaaaaa tcaggataag
     6481 gcgacgaagc cgcagacagt acaaatagta cggaaccgat tcactcggtg cttgagcacc
     6541 ttagagaatc gttctctttg agccaaggcg aggcaacgcc gtaccggttt ttgttaatcc
     6601 gctatattcc gccatctcta agatttacag cgatacacag gtaatttaag gaatgcccga
//
LOCUS       DAXHTR010000116         6603 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.116, whole genome shotgun sequence.
ACCESSION   DAXHTR010000116 DAXHTR010000000
VERSION     DAXHTR010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6603
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.116"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            95..1122
                     /locus_tag="ACFNML_001618"
                     /pseudo
     CDS             95..1122
                     /locus_tag="ACFNML_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Slam-dependent surface lipoprotein"
     gene            1153..3585
                     /gene="hupB"
                     /locus_tag="ACFNML_001619"
     CDS             1153..3585
                     /gene="hupB"
                     /locus_tag="ACFNML_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemoglobin-haptoglobin-utilization protein HupB"
                     /protein_id="HFC6392968.1"
                     /translation="MHIPFKPVLAAAAIAQAFPAFAADPAPQSAQTLNEITVTGTHKT
                     QKLGEEKIRRKTLDKLLVNDEHDLVRYDPGISVVEGGRAGSNGFTIRGVDKDRVAINV
                     DGLAQAESRSSEAFQELFGAYGNFNANRNTSEPENFSEVTITKGADSLKSGSGALGGA
                     VNYQTKSASDYVSEDKPYHLGIKGGSVGKNSQKFSSITAAGRLFGLDALLVYTRRFGK
                     ETKNRSTEGNVEIKNDGYVYNPTDTGGPSKYLTYVATGVARSQPDPQEWVNKSTLFKL
                     GYNFNDQNRIGWIFEDSRTDRFTNELSNLWTGTTTSPATGDYRHRQDVSYRRRSGVEY
                     KNELEHGPWDSLKLRYDKQRIDMNTWTWDIPKNYDTNGINGEVYHSFRHIRQNTAQWT
                     ADFEKQLDFSKAVWAAQYGLGGGRGDNANSDYSYFAKLYAPKILASNQAKITMLIENR
                     SKYKFAYWNNAFHLGGNDRFRLNAGIRYDKNSSSAKDDPKYTTAIRGQIPHLGSERAH
                     AGFSYGTGFDWRFTKHLHLLAKYSTGFRAPTSDETWLLFPHPDFYLKANPNLKAEKAK
                     NWELGLAGSGKAGNFKLSGFKTKYRDFIELTYMGVSSDNPNKPTYAPLSDGTALVSSP
                     VWQNQNRTAAWVKGIEFNGTWNLDSIGLPKGLHTGLNVSYIKGKATQNNGKETPINAL
                     SPWTAVYNLGYDAPSKRWGINAYATRTAAKKPSDTVHSNDDLNNPWPYAKHSKAYTLF
                     DLSAYLNIGKQVTLRAAAYNITNKQYYTWESLRSIREFGTVNRVDNKTHAGIQRFTSP
                     GRSYNFTIEAKF"
     gene            complement(4174..5808)
                     /gene="groL"
                     /locus_tag="ACFNML_001620"
     CDS             complement(4174..5808)
                     /gene="groL"
                     /locus_tag="ACFNML_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFC6392969.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAGLDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVISEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAIESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEEKPAMPDMGGMGGMGGMM"
     gene            complement(5902..6189)
                     /locus_tag="ACFNML_001621"
     CDS             complement(5902..6189)
                     /locus_tag="ACFNML_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFC6392970.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVE"
     gene            6418..>6603
                     /locus_tag="ACFNML_001622"
     CDS             6418..>6603
                     /locus_tag="ACFNML_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6392971.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLALAADEV"
BASE COUNT         1630 a         1923 c         1588 g         1462 t
ORIGIN      
        1 tttttgcacc gacccacaaa accagttgac atcatataac atttaaactg ataatcaaaa
       61 ctattattaa cacatcatca agattgagta tgcgatgaaa tacaaagccc tgtccttact
      121 gccgcttgcc gccgcccttg ccgcctgtgc cggggggggg tagccgaacc gcacgtcccc
      181 gtgtccatcc ccaccgccac gccgctgccc aaagacgagg taacgttatc aagcgataac
      241 ggcaaaatcg aaaacatcaa caccaccagc accggaagca catccggcat ttccattcag
      301 cagcgggaat atcaagtaac caattacggc gtcgaatcaa cagcaaaagc attcatcttc
      361 aaaacacctg gcggcgcgca atacaccctt tcatcctact ccgaccccat tgtccccagc
      421 tccttctccc ccgactacaa aattcctgac cgccacgcag ggcagcggct tgccgacggc
      481 agtcgcatct ttatctgctg cagcgactcc ggggcaaccg atgaagcgga aattaccaaa
      541 caagattata tgaaattcgg ggcgtggata ggccccaacg gcgaaatcga cctcttcgcc
      601 ggcggcttcc ccgtcggcaa aacgccacct cctgcgttct cctacggcag cagcacaccc
      661 gaaaccaaag gcacaggcaa aatcacttat caggtttggg gcatccgcgt cagaaacggg
      721 caatttgtta cttcctctta tactccgccg aaaagcggtt cttattatgg gacacttgcc
      781 aatacccccg tcctttcctt tatcaccgcc aatttcaaca gcaacaaact ggcaggcaaa
      841 atcctcggca acagcgatta cgggccggat gtggacattc aaaacgcaac gattaccggt
      901 ctgacctttt ctggcgatgc cacgtccggc ggcaaaaacg gcaacttgga aggcaagttt
      961 tttggaaaat tcgacagcag ctggggcaat gcagacacca gcataggcgg caaaatcact
     1021 tttaaagacg accgctccct cgataccgta ttcggcggtg tgagttatga gagaaaactt
     1081 gatgacacca gtgatatgag taccgaccat ctcaaaaaat aataacccat ccgcttttaa
     1141 aaaggttttc ttatgcacat ccctttcaag cccgtattgg ctgccgccgc catcgcccaa
     1201 gcgtttcccg cctttgcggc agaccccgcg ccgcagtccg cccaaacgct aaacgaaatc
     1261 accgttaccg gcacgcacaa aacccaaaaa ctcggcgaag aaaaaatccg ccgcaaaacc
     1321 ttagacaagc tcttggtcaa cgacgaacac gacctggtgc gctacgaccc cggcatttcc
     1381 gtcgtcgaag gcggcagggc gggttccaac ggctttacca tacgcggcgt ggacaaagac
     1441 cgcgtcgcca tcaacgttga cgggctggcg caggcagaaa gccgctcttc cgaagccttc
     1501 caagaattgt tcggcgcgta cggcaacttc aacgccaacc gcaacacttc cgagccggaa
     1561 aatttctcgg aagtaaccat caccaaaggc gcggactcgc tcaaatccgg cagcggcgca
     1621 ttgggcggcg cagtcaatta ccaaaccaaa tccgcaagcg attatgtttc cgaagacaag
     1681 ccctaccatt tggggataaa gggcggcagc gtcggcaaaa acagccaaaa attcagcagc
     1741 atcaccgccg ccggcaggct ctttggtttg gatgccttat tggtttatac ccgccgcttc
     1801 ggcaaagaaa ccaaaaaccg ctcgaccgag ggcaatgtcg aaattaaaaa cgacggatat
     1861 gtctataacc cgaccgatac aggcggaccc agcaagtacc tgacctatgt agccacaggg
     1921 gttgcgcgct cccaacccga cccacaggaa tgggtaaaca aaagcaccct gttcaagctg
     1981 ggctacaact tcaacgatca aaaccgtatc ggctggattt ttgaagactc ccgcaccgac
     2041 cgttttacca acgagctgtc taatttgtgg acgggtacga ccacatctcc cgcaacgggc
     2101 gactaccgcc accgccaaga cgtgagctac cgccgccgct ccggcgtcga atacaaaaac
     2161 gaattggaac acggcccgtg ggacagcctc aagctgcgct acgacaagca gcgcatcgat
     2221 atgaacactt ggacttggga catcccgaaa aattacgata caaacggcat caacggcgag
     2281 gtttaccatt cgttccggca tatccgccaa aacaccgcgc aatggactgc cgattttgaa
     2341 aaacaactcg acttttccaa agccgtttgg gcggcgcaat acggcttggg cggcggcaga
     2401 ggggacaatg ccaactcgga ttacagttat ttcgcaaaac tgtacgcccc caaaatcctc
     2461 gcttccaacc aagccaaaat cacgatgctg atcgaaaacc ggtcgaaata taaatttgcc
     2521 tattggaaca atgcgtttca cttgggcggc aacgaccgct tccgcctgaa tgcgggcata
     2581 cgctacgaca aaaacagcag cagcgcgaaa gacgatccga aatacaccac cgccatccga
     2641 gggcagattc cccatttggg ttcggaacgc gcgcacgccg gcttcagcta cggcacggga
     2701 tttgactggc ggtttaccaa gcatctgcac ttgttggcaa aatacagcac cggcttccgc
     2761 gcaccgactt cggacgaaac ctggctgctg ttcccacacc ccgatttcta cctgaaagcc
     2821 aacccaaacc tgaaagccga aaaagccaaa aactgggaat tgggtctggc gggcagcggc
     2881 aaagcgggca acttcaagct ctcgggcttc aaaaccaaat accgcgactt tatcgaattg
     2941 acgtatatgg gcgtttcgtc agacaaccct aacaagccca cctacgcccc gctttcagac
     3001 ggtacggcat tggtcagctc gcccgtttgg caaaaccaaa accgcaccgc cgcctgggtg
     3061 aaaggcatag agtttaacgg cacttggaac ctcgacagca tcgggctgcc caaagggctg
     3121 cacaccggcc tcaacgtcag ctacatcaag ggcaaggcaa cgcaaaacaa cggcaaagaa
     3181 acgcccatca acgcgctttc gccgtggacg gcggtttaca atttgggcta cgatgctccc
     3241 tccaaacgct ggggtatcaa cgcctacgcc acgcgcaccg ccgccaaaaa gccgtccgac
     3301 accgtccaca gcaacgacga cttgaacaac ccgtggcctt atgccaaaca cagcaaagcc
     3361 tatacgctgt tcgacctttc cgcctacctc aacatcggca aacaggttac gctccgcgcc
     3421 gccgcgtaca acattaccaa caagcagtac tacacttggg aatctttacg cagcatccgc
     3481 gagttcggca cggtcaaccg cgttgacaac aaaacccacg ccggcatcca acgctttacc
     3541 tcgccgggca ggagctacaa tttcaccatc gaagcgaagt tctaagctcc tgatgtgcca
     3601 atgccgtctg aaacccgatg atttgggttt cagacggcat tttttacggt cgcaccgccg
     3661 ccccctttca ggcttgtacc aagcccaatc ccgcaactct ccgtcattcc cgcgactctc
     3721 cgtcattccc acggaagtgg gaatctagaa acgcaaagct gcaagaattt atcggaaatg
     3781 actgaaactc aacgaacctg gattcccgcc tgcgcgggaa tgacgaagct atccgcacag
     3841 aaacctgcac cacgtcattc ccacgaaagt gggaatccag aacgtaaaat ctgaagaaac
     3901 cgttttatcc gatacgtttc cgcaccgaca gacctagatt cccgctttcg cgggaatgac
     3961 ggcggaaagg ttgctgtttt tccgataaat tcctgccgct cttcgttttt gggatggcgg
     4021 gaaataaaac aaaagcgcgc gtatcaaaaa acaaaaatgc aaagaacggc gttggcaatt
     4081 ttaaggtttg cgccttagcc gcacttattc gcaaaaaaaa ccgcacggcg ttgaccgtgc
     4141 ggcttgttgt ctgaaagctt cagacggcat ttcttacatc atgccgccca taccgcccat
     4201 gccgcccatg tcaggcatag caggtttttc ttcagggatt tcagcgatca tgcagtctgt
     4261 ggtcagcatc agaccggcga tagatgcggc gtgttgcagc gcggaacggg ttactttggc
     4321 aggatcgagt acgcccattt caatcatatc gccgtattcg ccgctgccag cgttgtaacc
     4381 gtagttgcct ttgccttcca acactttgtt cacgactacg ctgggttcgc cgcctgcgtt
     4441 ggcaacgatt tggcgcagcg gagactcaat ggcgcgcaag acgatttgta cgcctgcgtc
     4501 ttgatcggca ttgccggtgt gcaggttttc cagagcggca cgggcgcgca acagggctac
     4561 gccgccgcct gcaaccacgc cttcttcaac ggctgcgcgg gtagcatgca gcgcgtcttc
     4621 tacgcggtct tttttctctt tcatttcgac ttcggtagcg gcaccgactt tgattactgc
     4681 cacgccgcct gccagtttgg ccacgcgctc ttgcagtttt tctttgtcgt aatcgctggt
     4741 tgcggtttcg atttgttggc ggatttcggc aacacgcgct tcgatttggg ctgcgtcgcc
     4801 gaagccgtcg atgatggtgg tgttttcttt accgatttcg atgcgtttgg cttgacccaa
     4861 gtcgtccaaa gtcgcttttt ccagagacag gccgacttct tcggaaatca ccgtgccgcc
     4921 ggtcaggatg gcgatgtctt gcagcatcgc tttgcggcgg tcgccaaagc ccggagcttt
     4981 aacggcaacg gttttcagga tgccgcgaat gttgtttacc accaaagtcg ccaaggcttc
     5041 gccttctacg tcttcagcga taatcaagag cggacggctg gcttttgcca cttgttccaa
     5101 aacaggcaac aagtcgcgga tgttgctgat ttttttgtcg aacagcaata caaacggatt
     5161 gtccaagccg gcgatttgtt tttccgcatc gttgatgaag taaggagaca ggtagccgcg
     5221 gtcgaattgc ataccttcaa ccacgtccag ctcgttttcc aaagatttgc cgtcttcaac
     5281 ggtaatcacg ccttctttgc cgactttttc catcgcttcg gcaataatcg cgccgacttg
     5341 ttcgtcggag ttggcggaaa tcgagccgac ttgggcgatt tctttagaag tgtcgcaagg
     5401 tttggcgatg tttttcagct cttcaaccaa agcggcgacg gctttgtcga taccgcgttt
     5461 caggtcggtc gggttcatac cggcggtaac gtatttcata ccttcggcaa cgatggattg
     5521 cgccagtacg gtggcggtag tcgtaccgtc gcccgccacg tcgttggttt tggacgcgac
     5581 ttcttttacc atttgcgcgc ccatattttc aaacttgtct ttcagttcga tttctttagc
     5641 gacggttacg ccgtctttgg taatgtgcgg gccgccgaaa gcgcggtcaa ccaccacgtt
     5701 gcggcctttg ggacccaggg ttacgcgcac ggcgtttgcc agaatgttca cgccgtttac
     5761 cattttttga cggacttcat tgccgaattg tacgtctttt gctgccattt caattctcca
     5821 aaaatcatta aaactgtctg ataaaaccgt ttatgccgtc tgaagacggt ttgccgtttc
     5881 agacggcatc atcattcgca cttattcaac gatgccgaaa atatcttctt cgcgcattac
     5941 caacagctct tcgccgtcgg cttttacggt ttggccgctg tatttgccga aaatgatttt
     6001 gtcgccgact ttgacatcca gcggacggcg cgcgccgtct ttaccgattt tgcccgcgcc
     6061 cacggcgatg acttcgccca tatcgggttt ttcggcggcc gcacccggca aaacgatgcc
     6121 cgatgcggtt ttctcttcag cttccaagcg tttgacgacg acgcggtcgt gtaaaggacg
     6181 gatggtcata tctatgctcc gataaaatgg tttgaaaaca atcatctgcc cgaacggttc
     6241 ggacagatta aggatgaaaa ccggacagcc gtcaggcggc tgcccgtata agtcggcaaa
     6301 attagggtgt gtgcgggcaa attcaagtga ggcggaaaaa atttatttcc gccgtttttt
     6361 atagtggact aaatttaagg ggctgtacta gattagcaga tatgttaccc tcgaaatatg
     6421 aagataacgc actgcaaatt aaagaaaaaa gtacagaaag agctgctccg tttttttgta
     6481 ctcgaagtta ccgcccgttc tgccgccgat attttgggta tccatcccaa ttcggcagca
     6541 ctgttctacc gtaaaatccg cacggttatc aaccatcatt tggccctggc tgccgatgag
     6601 gtt
//
LOCUS       DAXHTR010000117         6578 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.117, whole genome shotgun sequence.
ACCESSION   DAXHTR010000117 DAXHTR010000000
VERSION     DAXHTR010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6578
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.117"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            129..1034
                     /gene="glyQ"
                     /locus_tag="ACFNML_001623"
     CDS             129..1034
                     /gene="glyQ"
                     /locus_tag="ACFNML_001623"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFC6392972.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
     gene            1111..1521
                     /locus_tag="ACFNML_001624"
     CDS             1111..1521
                     /locus_tag="ACFNML_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding protein YggL"
                     /protein_id="HFC6392973.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQTLIFGVQGSLHQAE
                     DDAEAFDRFMDELIAYLEVNDFGMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLHTTPLVDGFYGDDGWENYSETYKQAD"
     gene            1542..3605
                     /gene="glyS"
                     /locus_tag="ACFNML_001625"
     CDS             1542..3605
                     /gene="glyS"
                     /locus_tag="ACFNML_001625"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFC6392974.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAMKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGELLEDIINQAVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLSSVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEKTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDLTAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAALGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMAEDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            3622..4668
                     /locus_tag="ACFNML_001626"
     CDS             3622..4668
                     /locus_tag="ACFNML_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFC6392975.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWRNLDILIVDDGSTDGTLAIARRFQEQDGRIRILAQPRNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHRHG
                     KIWKKPTRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSVRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTLPAGAWLDFAADGRMRRLFTL
                     RQYFGILHRLLKNR"
     gene            4710..5537
                     /locus_tag="ACFNML_001627"
     CDS             4710..5537
                     /locus_tag="ACFNML_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6392976.1"
                     /translation="MQNHVISLASAAERRAHITDTFGVRGIPFQFFDALMPSERLEQV
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYITVFEDDVLLGEGAEKFLA
                     EDAWLQERFDPDSAFIVRLETMFMHVLTSPSGVADYGGRAFPLLESEHWGTAGYIISK
                     KAIRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            5549..5644
                     /locus_tag="ACFNML_001628"
                     /pseudo
     CDS             5549..5644
                     /locus_tag="ACFNML_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
     gene            5686..6528
                     /locus_tag="ACFNML_001629"
     CDS             5686..6528
                     /locus_tag="ACFNML_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HFC6392977.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHAVLWKQALDEGLPYVAVFEDDVLLGKDAEKFLA
                     EDTWLEERFDKDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
BASE COUNT         1685 a         1768 c         1732 g         1393 t
ORIGIN      
        1 actggtttaa atttaatcca ctatatcatg tatgccgtgt tttcaaatca gtctgtcttt
       61 gagataaacc tatccaaaca gcaatggaaa cagtaaaata aaccctttat tttatcgaat
      121 cataagccat gcttaccttc caacaaatca tcttcaaact gcaaaccttc tgggcagaca
      181 aaggctgcac cgtcatccaa cccttcgaca tggaagtcgg tgccggcaca tcccatcccg
      241 ccacctgcct gcgcgcgctc gggcccgagc cttggtttgc cgcctacgtc caacccagcc
      301 gccgccccaa agacggccgc tacggcgaca accccaaccg cctgcaacac tattaccaat
      361 tccaagtcgc cctcaaaccc gcccccgcca atatccaaga cctttatctc gactccctgc
      421 gcgaattggg catcgacccc aaagtgcacg acatccgctt cgtcgaagac gactgggaaa
      481 accccaccct cggcgcatgg ggcttgggct gggaagtttg gctcaacggc atggaagtaa
      541 cccagtttac ctatttccaa caagtcggcg gcatcgactg cacgcccgta ctcggcgaaa
      601 tcacctacgg catcgaacgc ttggcgatgt acctgcaagg cgtggaaaac gtctatgacc
      661 tcgtttgggc aaaaacgccg gacggcaaca ccgtaagcta cggcgacgtg taccaccaaa
      721 acgaagtcga acaatccacc tacaacttcg aatacagcga tgccgattgg ctgctgcgcc
      781 agttcaacga ctacgaagcg caagccaaac gcctgcttgc cgaagaaaac gccgccctcg
      841 ccttgcctgc ctacgagctg gttctcaaag cggggcatac gttcaatctt ttagacgcac
      901 gcggcgcgat ttccgtaacc gagcgggcaa cttatattgg acgtattcgt gcattgagcc
      961 gcgccgtggc gcaaaaatat gttgaaagcc gcgaaaaact gggcttccct ttaatgaagg
     1021 caaatgctgc ctaagggggc gacgattttt tacgttcggg cggcacgata aggcagccgt
     1081 ccgtaaacca ttatatattt taggaaaatc atgcctgtct atttccgcac cccaagcagg
     1141 aaacgcctgc acaaaatgaa ccgccgccag cataaaaagc tgcatttggg cgagtttcaa
     1201 acattgattt tcggcgttca aggcagtttg caccaagctg aggacgatgc cgaagcgttc
     1261 gaccgcttta tggacgagct gattgcgtat cttgaggtaa acgactttgg aatggcgggc
     1321 gggggcgatt gcggcgattt ttcgtgcctg tttcaaaaat ccggtgcccg aagcttgggc
     1381 gaaaccgaga aaaaagccat tatctcttgg ctggcaggcc gcgaagatgt cgccactttg
     1441 cacaccaccc cgctggttga cggtttttac ggggatgacg gctgggaaaa ttacagtgaa
     1501 acctataaac aggcagatta accaaattta aagaaagccg aatgatgacc caaacccttt
     1561 taatcgaact ccttaccgaa gaactcccgc ccaaagcact gaataatctg ggcaaccatt
     1621 ttgccgccgc cgttgccgaa ggcttggaaa aagcgcaact ggttgacggt gcagccgaat
     1681 ttacggctta tgcctcgccg cgccgtttgg cggttcaagt caaaaacgtt aaagccgttc
     1741 aggccgacca aaaaatcgtg aaaaaaggcc ctgccgtggc gaatgccatg aaagacggtg
     1801 cgccgaccaa ggctttggaa ggtttcgcac gcggtgcggg cgcgaaaatc gaagacttga
     1861 ccatcgtcca cgacggcaaa caggatgtgt acgcctacga atacgtccaa accggcaaac
     1921 cgctgggcga acttttggaa gacattatca atcaagccgt taagaaactg ccgattccga
     1981 aagtgatgcg ttggggcagc agcacgttta ccttcgtgcg ccctgttcac gggctgattg
     2041 tgctgcacgg cggcgacatc gtaaacgtca gcgttttggg tctgcaaagc agcaatcaaa
     2101 ccttgggaca ccgcttcttg tccaacggcg aaatcaccat tgaaaacgcc gacagctacg
     2161 ccgaacaaat gcgcgggcaa ggcaaagtcg tcgcttcgtt tgccgagcgc aaagccgcga
     2221 ttcagacggc attggaaggg caggcgcgca gtctgaacgc cgccgtcgcc gccgatgaag
     2281 cattgttgga cgaagtaacc gcgctggtcg aatggcctgt tgttttggaa gccggttttg
     2341 aggaacattt cctcgccgtg ccgcaggaat gcttgatttt aacgatgcag caaaaccaaa
     2401 aatactttcc gctgctcgac caaaacggca agctgatgaa ccgcttcctg ctggtttcca
     2461 acctgcaaac cgaagacccg tcgcacatca tccgaggcaa cgaacgcgtt ttgcgcgcgc
     2521 gcctgtctga tgccgagttc ttctacaaac aagaccaaaa agcgactttg gaaagccgcc
     2581 tgccgaaatt gtccagcgtg gtgtaccaca acaaaatcgg ctcgcaagcc gaacgcatcg
     2641 aacgcctgca aagcattgcc gcccacatcg ccaaagcatt gggcgcggat gccgccgctg
     2701 ccgaacgcgc cgcgcgtctt gccaaagccg acttggtaac cgaaatggtt ggcgagttcc
     2761 ccgaactgca aggcacgatg ggcaaatact atgcacgttt ggacggcgaa accgaagaaa
     2821 tcgccgaagc catcgagcag cactatcagc cgcgttttgc cggcgacaag ctgcccgaaa
     2881 gcaaaattgc cgccgccgtg gcactggcgg acaagctgga aaccctggtc ggcatttggg
     2941 gcatcggtct gattccgacc ggcgacaaag acccctacgc cctgcgccgc gccgccttgg
     3001 gtattttgcg gatgctgatg cagtacggtt tggacgtgaa cgaactgatt cagacggcat
     3061 tcgacagctt ccccaaaggt ttgctcaacg aaaaaacgcc gtctgaaacc gccgacttta
     3121 tgcaggcgcg ccttgccgtg ttgctgcaaa acgattatcc gcaagacatc gtcgccgccg
     3181 tactcgccaa acagccgcgc cgtttggacg atttgaccgc caaactgcaa gccgttgccg
     3241 cgttcaaaca gctgcccgaa gccgccgcgc tcgccgccgc caacaaacgc gtgcaaaacc
     3301 tgctgaaaaa agccgatgcc gcgttgggcg cggttaacga aagcctgttg caacaggacg
     3361 aagaaaaagc cctctttgcc gccgcgcaag gcttgcagcc gaaaatcgcc gccgccgtcg
     3421 ccgaaggcaa tttccaaacc gccttgtccg aactggcttc cgtcaaaccg caagtcgatg
     3481 cattctttga cggcgtgatg gtaatggcgg aagatgccgc cgtaaaacaa aaccgcctga
     3541 acctgctgaa ccgcttggca gagcaaatga acgcggtagc cgacatcgcg cttttgggcg
     3601 agtaaccgtt gtacagtcca aatgccgtct gaagccttca gacggcatcg tgcctatcgg
     3661 gagaataaat tgcagccttt agtcagcgta ttgatttgcg cctacaacgt agaaaaatat
     3721 ttcgcccaat cattagccgc cgtcgtgaat cagacttggc gcaacttgga tattttgatt
     3781 gttgatgacg gctcgacgga cggcacactt gccattgccc ggcgtttcca agaacaggac
     3841 ggccgcatcc gcatcctcgc gcagccgcgc aattccggcc tgattccctc tttaaacatc
     3901 gggctggacg aattggcaaa gtcaggaatg ggggaatata ttgcacgcac cgatgccgac
     3961 gatattgccg cccccgactg gattgagaaa atcgtgggcg agatggaaaa agaccgcagc
     4021 atcatcgcga tgggcgcgtg gctggaagtt ttgtcggaag aaaaggacgg caaccggctg
     4081 gcgcggcatc acaggcacgg caaaatttgg aaaaagccga cccgacacga agatattgcc
     4141 gactttttcc ctttcggcaa ccccatacac aacaacacga tgattatgag gcgcagcgtc
     4201 attgacggcg gtttgcgtta caacaccgag cgggattggg cggaagatta ccaattttgg
     4261 tacgatgtca gcaaattggg caggctggct tattatcccg aagccttggt caaataccgc
     4321 cttcacgcca atcaggtttc atccaaatac agcgtccgcc aacacgaaat cgcgcaaggc
     4381 atccaaaaaa ccgccagaaa cgattttttg cagtctatgg gttttaaaac ccggttcgac
     4441 agccttgaat accgccaaat aaaagcagta gcgtatgaat tgctggagaa acatttgccg
     4501 gaagaagatt ttgaacgcgc ccgccggttt ttgtaccaat gcttcaaacg gacggacacg
     4561 ctgcccgccg gcgcgtggct ggattttgcg gcagacggca ggatgcggcg gctgtttacc
     4621 ttgaggcaat acttcggcat tttgcaccga ttgctgaaaa accgttgaaa aacgccgctt
     4681 tatccaacag acaaaaaaca ggataaatta tgcaaaacca cgttatcagc ttagcttccg
     4741 ccgcagaacg cagggcgcac attaccgata ccttcggcgt gcgcggcatc ccgtttcagt
     4801 ttttcgacgc actgatgccg tctgaaaggc tggaacaggt aatggcggaa ctcgtacccg
     4861 gtttgtcggc gcacccctat ttgagcggag tggaaaaagc ctgctttatg agccacgccg
     4921 tattgtggaa gcaggcattg gacgaaggtc tgccgtatat caccgtattt gaggacgacg
     4981 ttttactcgg cgaaggcgcg gagaaattcc ttgccgaaga cgcttggctg caagaacgct
     5041 ttgaccccga ttccgccttt atcgtccgct tggaaacgat gtttatgcac gtcctgacct
     5101 cgccctccgg cgtggcggac tacggcgggc gtgcctttcc gctgttggaa agcgaacact
     5161 gggggacggc gggctatatc atttccaaaa aagcgatacg gtttttcctg gacaggtttg
     5221 ccgccctgcc gcccgaaggg ctgcaccccg tcgatctgat gatgttcagc gattttttcg
     5281 acagggaagg agtgccggtt tgccagctca atcccgcctt gtgcgcccaa gagctgcatt
     5341 atgccaagtt tcacgaccaa aacagcgcat tgggcagcct gatcgaacac gaccgcctcc
     5401 tgaaccgcaa acagcaatgg cgcgattccc ccgccaacac attcaaacgc cgcctgatcc
     5461 gcgccttgac caaaatcagc agggaaaggg aaaaacgccg gcaaaggcgc gaacagttca
     5521 ttgtgccttt ccaataaaag gaggagagat ggacatcgta tttgcagcag acggcaggat
     5581 gcggcggctg tttaccttga ggcaatactt cggcattttg cgccgattgc tgaaaaaccg
     5641 ttgaaaaacg ccgctttatc caacagacaa aaaacaggat aaattatgca aaaccatgtt
     5701 atcagcttag cttccgccgc agaacgcagg gcgcacattg ccgcaacctt cggcgcgcgc
     5761 ggcatcccgt ttcagttttt cgacgcactg atgccgtctg aagaactgaa tcggatgatg
     5821 gcggaattgg tgccgggatt ggcaaaacag cacctgttga gcgaagtgga aaaagcctgc
     5881 tttatgagcc acgccgtatt gtggaaacag gcattggatg aaggtctgcc ctatgttgcc
     5941 gtatttgagg atgatgtcct gcttggcaaa gacgcagaaa agttccttgc cgaagatact
     6001 tggttggaag agcgttttga taaggattct gcctttatcg ttcgtttgga aacgatgttt
     6061 gcgaaagtta ttgtcagacc ggataaagtc ctgaattatg agaaccggtc atttcctttg
     6121 ctggagagcg aacattgggg gacggctggc tatatcattt cgcgtgaggc gatgcggttt
     6181 ttcttggaaa ggtttgccgt tttgccggca gagtggatta aagcggtaga ttggatgatg
     6241 tttacttatt tctttgataa ggaggggatg cctgtttatc aggttaatcc cgccttatgt
     6301 acccaagaat tgcattatgc caagttcctc agtaaaaaca gtatgttggg tagcgatttg
     6361 gaaaaagata gggaacaaga aagaagacac cgccgttcgt tgaaggtgat gtttgacttg
     6421 aagcgtgctt tgggtaaatt cggtagggaa aagaagaaaa gaatggagcg tcaaaggcag
     6481 gcggagcttg agaaagctta cggcaggcgg gtcatatcgt tcaaatagtt tgtgtaaaat
     6541 atagcggatt aaaattagaa atggatacac tgtcattc
//
LOCUS       DAXHTR010000118         6493 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.118, whole genome shotgun sequence.
ACCESSION   DAXHTR010000118 DAXHTR010000000
VERSION     DAXHTR010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6493
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.118"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..243)
                     /locus_tag="ACFNML_001630"
     CDS             complement(<1..243)
                     /locus_tag="ACFNML_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392978.1"
                     /translation="MPSETFPFQTAWFYSNLNRADRSQKHTFAIPAQALPLYAPNPKP
                     PHSRRHYSGLNFRGCTRLADMLPSKYEDNALQIKEKS"
     gene            complement(268..2325)
                     /gene="ppk1"
                     /locus_tag="ACFNML_001631"
     CDS             complement(268..2325)
                     /gene="ppk1"
                     /locus_tag="ACFNML_001631"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFC6392979.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDVQNLRAAIQNELHDREYGDGVRLEVADTCPSYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRATPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(2494..2937)
                     /locus_tag="ACFNML_001632"
     CDS             complement(2494..2937)
                     /locus_tag="ACFNML_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HFC6392980.1"
                     /translation="MPFRLFLGILCDLSMVAPVYAQGQPDAVNAYIQKKKVIVNTSKA
                     ELCFADDRQCHPVLIGVATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(2944..3459)
                     /locus_tag="ACFNML_001633"
     CDS             complement(2944..3459)
                     /locus_tag="ACFNML_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFC6392981.1"
                     /translation="MKIKQIVKPGLAVLAAGILSACATKSNVKADGATDNPVFPKPYS
                     VTLDNKRGTFPTYDELDQMRPGLTKDDIYKILGRPHYDEGMYGVHEWDYLFHFHTPGV
                     GIDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            3726..6356
                     /gene="leuS"
                     /locus_tag="ACFNML_001634"
     CDS             3726..6356
                     /gene="leuS"
                     /locus_tag="ACFNML_001634"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFC6392982.1"
                     /translation="MQEQYQPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKLLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYAKFLQVYTTRPDTLMGATYVAVAAEHPL
                     AAAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFAAKYNLPKKQVIAVGDNAFDANRWQEWYADKENGV
                     LVNSGDLDGLDFQTAFDAVAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     KCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFKRLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGSQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLEAAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPTVDEASLVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
BASE COUNT         1419 a         1762 c         1894 g         1418 t
ORIGIN      
        1 acttttttct ttaatttgca gtgcgttatc ttcatatttc gagggtaaca tatctgctaa
       61 tctagtacag cccctaaaat ttaatccact ataatgccgt ctggaatgcg gcggcttggg
      121 gttgggagcg tataggggaa gtgcttgtgc aggaatggcg aaggtgtgtt tttgactccg
      181 gtccgccctg tttagattgc tgtaaaacca tgccgtctga aacggaaagg tttcagacgg
      241 catggttttt gattggggcg gtgcgggtca gcctccgagc agtgtccaca aatcgttttg
      301 caggtcggct tcgctttcgc cctcggcggg cgtggcgcgg atatagccgc cgtcgggctg
      361 catcagccac gcgtgcgtat tgtctgccaa tgccatttcc aaaccttcgc ggatgacgcg
      421 ttttttgagt tcgggcgcgg taatcggcgc ggcggtttcg atgcggcgga agaagttgcg
      481 ccccatccaa tccgcgctgg agataaaggt atcgtccgcg ccgttgttgt ggaagcaata
      541 cacgcgcgcg tgttcgagct gcctgccgat gatggagcgg acgcggatgt tttcggacaa
      601 gccttttaca cccgggcgca aggtgcacat accgcgcaca atcaaatcga tttgtacgcc
      661 tgccgcgctt gcccgataca gggcttcgat gacggtcggt tcgatgagcg aattcatctt
      721 ggcggtaatc cgcgccggtt tgccggcttt tgcgtgttcg gtttcgcgtg cgatgcggtc
      781 gataaccatt ttgtgcaggg taaacgggct ttggtagagt ttgttcagcc gcccgggttt
      841 gcccaagcct gtgatttcca taaacaatgt gttcacatcg gcggtgattt gttcgtcggc
      901 ggtaatgatg ccgaagtcgg tgtagatgcg cgatgtgcct tggtggtagt tacccgtgcc
      961 gaggtgggcg taacgcttga gtacgccgtc ttcgcggcgg atgaccagtg ccatcttggc
     1021 gtggactttg tagccgaaca cgccgtacac gacgtgcgcg cccgcctctt cgagctgctt
     1081 cgcccagttg acgttgttgg cttcgtcaaa acgcgccatc agttcgacga cgacggttac
     1141 ttgtttgccg gcgagtgccg ccttcatcag ggcgcggacg agttcggaac gcgtgccggt
     1201 gcggtaaatc gtcattttga cggcaagcac gtcggggtcg gcggcagctt cgcgcatcat
     1261 ttcgacaacg gggtcgaacg attggtaggg gtggtgcagc aggatgggcg attggcgcac
     1321 caaatcgaat atggacgcgg ttttgcccaa ggctttcagt ctgcccggcg tgtgtgtggg
     1381 aaatttcaaa tcggggcggt tgactaggtc ggggacggcg ttgaggcgca cgaggttgac
     1441 cgggcctttg acctgataga gttcggcggc ggtcagtttg aattgcgcga gcagaaagtc
     1501 gcggatgtag gagggacagg tgtcggcgac ttcgagccgc acgccgtcgc cgtattcgcg
     1561 gtcgtgcagt tcgttttgaa tggcggcgcg gaggttttgt acgtcttctt cgtcaacggt
     1621 caagtcgctg tcgcgcgtga ggcggaactg gtggcagcct ttgacgttca tgcccgggaa
     1681 gagtttgccg acgtgggcgt gcaggatgga ggagaggaag acgaagccgt gtccgccgcc
     1741 acacagttcg gacggcaggg gaacaacgcg cggcaggatg cgcggtgcct gcacaatcgc
     1801 catccccgaa ggcctgccga acgcgtccgt gccgtcgagt tcgacggcga agttgagcga
     1861 tttgttcagc ggtcgcggga aggggtggga cgggtcgagt ccgatggggg tcagaatcgg
     1921 cagcaattcg cggtcgaaat agctttcgat ccactttttc tgcgcgcccg tccagttgcg
     1981 gcggcggtaa aaatggatgc cttcttgtgc cagctcgggc tgaaggacgt tgttgaacag
     2041 gtcgtactgg tgccgtatca gggagcgcgc cgcttcggta acgtcggcga tggtttcaga
     2101 cggcattttg ccgttgtcca gcctgcgccg ggggtgcagc ttgttttcac gcttgagcca
     2161 cgccatgcgg acttcgaaaa actcgtcaag attggacgac acgatgcaca ggaagcgcag
     2221 gcgttccaaa agggggacgt ttttgtcttc cgcctgtgcc aacacgcggc ggttgaatgc
     2281 cagcaggctt agttcgcggc agaggatgcg gttttgttcg ggcataaggt tctcccaaag
     2341 atggttgttg ttcggtcgga gcgggcaggc aatgccgtct gaaggcttgg cggcatctgc
     2401 gccggtttca gacggcatga cgtggcggac agtctgtttc aggcatcggg gcaatgtgaa
     2461 aacagatacc ggcgcgtgcg gtatccgtct gtttcaaatc acttccaaca caaaataatg
     2521 acgcagtttt tcgtacaccg catcgctgac gttgatgcag ccgttggtca taatcctgtc
     2581 ggacacggac ggggaggcga tgcgttcgtt ccgcctttcc gacggtatct gattccaaac
     2641 gcggtgcagg gcgaaaagaa aatcaccctc ctgcttgaaa ccgatgactt cgccgccgta
     2701 tccaggcttg tcggtactgt tcagcgtcag cccgaacgtc cccttgggcg tggcaacacc
     2761 gatgaggacg gggtggcact gcctgtcatc ggcaaaacag agttccgctt tggatgtgtt
     2821 tacgatgact tttttctttt gaatataagc gttgaccgca tccggttgcc cctgtgcata
     2881 tacgggggca accatcgata ggtcgcacaa tatccctaaa aacaggcgga acggcatgtc
     2941 ggattattgg cggatacgtt tcggttttgc cggcacgatt tcgcggatga tgacttgcgg
     3001 ctcggctttg ggtgcgggcg gcggacaggc ggcatctttc gggaagacgg ggttccagta
     3061 gaagctgcgg gcaaatttgt ctttatcgaa aatcactttg tattggcagg tagtaacatc
     3121 ttctacgccg gacgtgtttt cagggtcgat acctacgccc ggggtatgga agtggaacag
     3181 gtaatcccat tcgtgtacgc cgtacatgcc ttcgtcgtaa tgcgggcgac ccaggatttt
     3241 gtagatgtcg tctttggtca ggccggggcg catctgatcc agttcgtcat aggtcgggaa
     3301 tgtgccgcgc ttgttgtcga gcgttacgga atagggtttc gggaaaaccg gattgtcggt
     3361 cgctccgtcg gctttgacgt tgcttttggt tgcgcaggca gacagaatgc ccgccgccaa
     3421 tactgccaag cccggtttga cgatttgttt gattttcata tgcagaatcc ttttttaccc
     3481 gatgcccgtc tgccttttgt tcggaagagc cgcatcagga gagttttaaa catttgattt
     3541 tggtttgtaa tattagcata aaaaacgtgc gtatcagtaa acgcggtgta tttgtacggc
     3601 atacggaatg atgcgcgtgc gaatttacgc atcctgccgg caatttgccg attcgccgac
     3661 atcggcaacc tgttataatt cctcctttaa attcctaacg ttttcaagcg aaaaacagaa
     3721 tgactatgca agaacaatat caacccgccg ccatcgagcc tgcggcgcag aaaaaatggg
     3781 acgacgcccg tattttcaac gtctccgaag acgcttccaa acccaaatac tactgcctct
     3841 ccatgttccc ctaccccagc ggcaagctgc atatggggca tgtgcgcaac tacaccatcg
     3901 gcgacgtatt gagccgcttc aaacttttaa acggcttcaa cgtcatgcag cctatgggtt
     3961 gggacgcgtt cggcatgccg gcggaaaacg cggcgatgaa aaacaacgtc gcccctgccg
     4021 cttggacgta cgataacatc gaatacatga aaacccagct caaaagcctg ggttttgcga
     4081 ttgactgggc gcgcgaaacc gcaacctgca aacccgaata ctaccgctgg gaacaatggc
     4141 tgtttaccaa gctgtttgaa aaaggcatcg tttatcgcaa aaacggcacg gtaaactggg
     4201 acccggtcga ccaaaccgtc cttgccaacg agcaagttat cgacggacgc ggttggcgtt
     4261 cgggcgcgtt gatcgaaaaa cgcgaaatcc cgatgtatta cttcaaaatc accgattacg
     4321 ccgaagagct gctcaacgac ttggacaagc tggaacactg gcctgaacaa gtcaaaacca
     4381 tgcagcgcaa ctggatcggc aaatctcgcg gcatgaccgt gcgctttgct gtttcagacg
     4441 acagcaaaca aggcttggaa ggcgattacg cgaaattcct gcaagtttat accacccgcc
     4501 ccgacacgct gatgggtgcg acttatgttg ccgttgccgc cgagcatccg ctggcagccg
     4561 ccgcagccgc cgacaaaccc gaattgcagg catttattgc cgaatgcaaa gccggttcgg
     4621 ttgccgaagc cgatatggcg acgatggaga aaaaaggcgt gccgaccggt cgctacgtcg
     4681 tcaacccgct caacggcgac aagctggaag tgtggattgc caactatgta ttgtggggct
     4741 acggcgacgg cgcagtgatg gcggttccgg cgcacgacga acgcgatttt gagttcgccg
     4801 ccaaatacaa tctgcctaaa aaacaagtca ttgccgtcgg cgacaacgca ttcgacgcaa
     4861 accgatggca agaatggtat gccgacaaag aaaacggcgt attggtcaac agcggcgact
     4921 tggacggctt ggattttcag acggcattcg atgccgttgc cgccaagctg caaagccaag
     4981 gcgcgggcga accgaaaacc caataccgcc tgcgcgactg gggcatttcg cgccaacgct
     5041 actggggctg tccaattccc atcgtccatt gtgaaaaatg cggcgacgta cccgtcccag
     5101 ccgaccaact gcccgtcgtt ctgcccgaaa acgtcgtacc cgacggcatg ggttcgccgc
     5161 tggcaaaaat gcccgagttt tacgaaactg cctgcccgtg ctgcggcggc gcggcgaaac
     5221 gcgaaaccga caccatggac accttcatgg aatcaagctg gtatttcttc cgctatatgt
     5281 cgcccaagtt ttcagacggc atggtatcgg cagaagccgc gaaatactgg ggcgcggtcg
     5341 accaatacat cggcggtatc gaacacgcga ttttgcatct cttgtacgcg cgtttcttca
     5401 ccaaactgat gcgcgacgaa ggtttggtca atgttgacga accgttcaaa cgcctgctca
     5461 cgcaaggcat ggtcgtctgc gaaacctact accgcgaaaa cgacaaaggc ggcaaagact
     5521 ggatcaaccc cgccgatgtc gagctgacgt tcgacgacaa aggccgcccc gtttccgccg
     5581 tcctcaaagc cgacggcctg cccgtcgtca tcagcggcac ggaaaaaatg tccaagtcca
     5641 aaaacaacgg cgtcgatccg caagaactga ttaacgccta cggcgcggac accgcccgcc
     5701 tgttcatgat gtttgccgca ccgcccgaac agtccctcga atggagcgac agcggcgtcg
     5761 aaggcgcaca ccgcttcctg cgccgcctgt ggcgcaccgt ttacgaatac ctgaaacaag
     5821 gcggcgcggt caaagcgttc gcaggcagcc aagatggttt gtctaaagaa ctcaaagacc
     5881 tgcgccacaa actgcattcc actatcgcca aagtcagcga cgactacggc cgccgccagc
     5941 agttcaacac cgccatcgcc gccgtgatgg aattgctcaa ccaatacgac aaaaccgaca
     6001 ccggcagcga acaaggccgc gccgtcgccc aagaagtatt ggaagccgcc gtacgtctgc
     6061 tgtggcccat cgtgccgcac atctgcgaaa ccctgtggag cgaattgaac ggcgcgaaat
     6121 tgtgggaagc aggctggccg acagtcgacg aagcctcctt ggtcaaatcc gaaatcgaag
     6181 tcatggttca agtcaacggc aaactgcgcg gaaaaatcac cgtcgccgcc gacgcatcca
     6241 aagccgacct cgaagccgcc gcactcgcca ccgagggcgc agtgaaattc atggaaggca
     6301 agcccgccaa gaaaatcatc gtcgtgccgg gcagactggt gaacatcgtc gtctaaccgc
     6361 ttttaaggtt tagccacacg gataaaggcc gagacctttg caaaattccc ttccctcccg
     6421 acagccgaaa cccaaacaca gattttcggc tgttttcgcc ccaaatacct cctaattcta
     6481 cccaaacgcc ccc
//
LOCUS       DAXHTR010000119         6466 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.119, whole genome shotgun sequence.
ACCESSION   DAXHTR010000119 DAXHTR010000000
VERSION     DAXHTR010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6466
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.119"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(90..1886)
                     /locus_tag="ACFNML_001635"
     CDS             complement(90..1886)
                     /locus_tag="ACFNML_001635"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HFC6392983.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFIH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSFLL
                     IGKDNAVLFTEQCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGTNPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFVSLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVAAPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
     gene            1942..2283
                     /locus_tag="ACFNML_001636"
     CDS             1942..2283
                     /locus_tag="ACFNML_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6392984.1"
                     /translation="MPSEKHNHLIKSPACCKTDGLIGWCVFARKKRCKVVCCKILGIK
                     YRFMPFFGSNMENIVKFEHEVLIVCVGRYLDYVTIRVFIKISLPSGILFLKTVGFGFV
                     WGQSRPNWSNR"
     gene            2344..3597
                     /locus_tag="ACFNML_001637"
     CDS             2344..3597
                     /locus_tag="ACFNML_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NRAMP family divalent metal transporter"
                     /protein_id="HFC6392985.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCIISATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQPDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            3752..5263
                     /gene="lysS"
                     /locus_tag="ACFNML_001638"
     CDS             3752..5263
                     /gene="lysS"
                     /locus_tag="ACFNML_001638"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFC6392986.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHTQYGEIGKEELDPQGIPVKIAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEILDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANGESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASRTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIIDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKSQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(5340..5528)
                     /locus_tag="ACFNML_001639"
                     /pseudo
     CDS             complement(5340..5528)
                     /locus_tag="ACFNML_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311460.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5532..6410)
                     /locus_tag="ACFNML_001640"
     CDS             complement(5532..6410)
                     /locus_tag="ACFNML_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HFC6392987.1"
                     /translation="MARRSKTFEEAAAEVEERFGHRGIKLVEFEGTAKPCVINCPKHG
                     NQTCSRYSNMFIGSSWGCPSCGNEQAAKAGIATLRKNHIALEMLKQAVTGMTKQERIT
                     TQAYNEMTKSVAGSNSIVLNDVQGDTTINNHHTHTHNHSDADGKALSMRLTPRPLLSD
                     RQAAAFARTGKLTGSFDLFASVVAPSQYTFAVAMPDTSMSPVIEKGDLVVVEPRMRPA
                     DEDIVLIELSDKRLVVAHLVIDIAGRMLIYQTGRPSEAFDLPEGSTILGVVLEVKKRF
                     MSAAQARRRVDSDYHP"
BASE COUNT         1396 a         1679 c         1852 g         1539 t
ORIGIN      
        1 cttgcggcaa gatgaaaacg gtgcgggatg ttttgggaaa ccaatgccgt ctgaagggct
       61 ttcagacggc attttttgcg ccgtgctgtt taacgcgcca gcggttcggt gcgtttgatc
      121 agccacgctt ttgccgcgcc ttcggtcagc ggctcgaggc ggcggcggac ttcggcgtgg
      181 tagcggttga cccagtcgat ttcgccgtcg gtcatgaggg cggtgtccat caggcgggtg
      241 tcgatggggc agagggtcag ggtttcaaaa cagaggaagc tgccgaattc ggtttcttga
      301 ggggcggcga cggcttggtt ggcggcaagg ttttcaatgc ggatgcccca ttttcccggg
      361 cggtagagtc cgggttcgat ggaggtaacc atgccttttt tcatggcggt ttcgggcgtg
      421 gcgggggcgg cgaaggcgat gcgctgcggg ccttcgtgga cgttgaggaa atagcctacg
      481 ccgtgtccgg tgccgtggcc gtagtcgcat tgcgcctgcc acaggggttt gcggcaaatc
      541 gcatcaatca gcggcgaggg gatgttttct gggaacacgg cttcggcaag cgcgatatgg
      601 gctttgagaa cgagggtgtt gtcgcgtttt tgttcggcgg tgggtgtgcc gacagggacg
      661 acgcgggtga tgtcggtcgt gccgcctttg tattgcgcgc cggagtcgat gagcagcagc
      721 ccgttgccgc tgatggtgct gtggctttcg ggcgttgcgc tgtaatgcgg cagggcgccg
      781 ttggcgttga agcctgcgat ggtgtcgaaa ctcaatgaca caaaacccgg gcgcacgctg
      841 cggtggcggt aaagcatggt gtccacgtcg atttcggtca ggctgccgcc gttgccgatg
      901 atgtcttcaa actcggcgaa gaaaccgcac aacgccgcgc cgtcgtgttc catcgcttcg
      961 cggatgcggg cgatgtcggc ttcggatttg caggatttga acagcgtgga cgggttggtg
     1021 ccttcgatta ggcgtacgct ttcgggcagg cgcacgagcg tactgacggc ggttttgttc
     1081 ggttcgataa gcagcgcgcc gccgatttgc gcgagcttgt cggcaacttg ggcgtaaggt
     1141 tcgaccgtga tgccggcggt ttgcagcgcg gcggcggctt cggcgttcag acggcattgt
     1201 tcggtaaaca ggacggcgtt gtctttgcca atcagcagga aggacacgaa aacgggattg
     1261 aaaggcacgt cgctgccgcg caggttggtc agccaagcga tgtcgtcaag cgatgaaacc
     1321 aagtggtaat ccgcgccttt ttccgccatc acggcgcgca cgcgggcgag tttttcggcg
     1381 gcggtttcgg agacgtaggc ggggtcgtgg atgaacacgg tttcggcggg gatagcggga
     1441 cggcttgtcc acacttggtc aagcagatcg tccgggtgtt cgatgcggat gtttttggcg
     1501 gtcagggatt gcgccaaagt gcgtttgccg gtgagcgaga ccatatcgga agggatgccg
     1561 acggcggcgt tttcgggcag gtttgccgcg agccattcgt tatacggcgg cacttgcccg
     1621 cttttttgca gttcgatgcc gctgcccgca agctgcttgg cggcttgttc ccaatagcgg
     1681 ctgtccaccc atacgcccgc ttcatcggtg gtcaggacaa acgtgccgac cgagccggta
     1741 aagcccgata attcgcggcg cgcctgccaa tgctcgggca ggtattcgga caggtggggg
     1801 tcggcggaag ggatgacgag cgcgtccaag ccttgcgcct tcatggcttc gcgcaatgcg
     1861 gacaggtaat tcgatacggt attcataaca gttcctccaa tcgggttttg cggcttcaga
     1921 cggcatggac ggaaatctga aatgccgtct gaaaaacata accatcttat caaatcgcca
     1981 gcctgctgca aaacggacgg gctgatcgga tggtgcgtgt ttgcgcgaaa aaagcgatgt
     2041 aaggttgttt gttgtaaaat tttaggcata aaatatcgtt ttatgccttt ttttggaagt
     2101 aatatggaaa atatagtcaa gtttgagcat gaagttctaa tagtgtgtgt tggcaggtat
     2161 ttggattacg ttacaatacg tgttttcatc aaaatttccc taccgagcgg tatcctgttt
     2221 ttgaagacgg taggatttgg gtttgtttgg gggcaatccc gcccaaattg gagcaataga
     2281 tagggctgtg tccgaatatg cggctctgtg tgttgaaaca cagttaaaaa tacggaagac
     2341 tttatgtccg aacaacatat ttcgacttgg aaaagtaaaa tcaacgcatt gggtccgggg
     2401 atcatgatgg cttcggcggc ggtcggcggt tcgcacctga ttgcctcgac gcaggcgggc
     2461 gcgctttacg gctggcagat cgcgctcatc atcatcctga ccaacctctt caaatacccg
     2521 tttttccgct tcagcgcgca ttacacgctg gacacgggca agagcctgat tgaaggttat
     2581 gccgagaaaa gccgtgttta tttgtgggtt ttcctgattt tgtgcatcat ttccgccacg
     2641 attaacgctg gcgcggtcgc catcgtaacc gccgccatcg tcaaaatggc gattccctcg
     2701 ctgatgtttg atgccggcac ggttgccgcc ttgattatgg catcctgcct gattattttg
     2761 gtgagcggac gttaccgcgc tttggatcgc gtttccaaaa tcatcatcgt tactttgagt
     2821 atcgccacgc ttgccgccgc cggcatcgct atgtcgcgcg gtatgcagat gcagccggac
     2881 tttatcgagc cgacaccgtg gacgcttgcc ggtttgggct tcctgatcgc gctgatgggc
     2941 tggatgcccg cgccgattga aatttccgcc atcaattctt tgtgggtaac cgaaaaacaa
     3001 cgcatcaatc cttccgaata ccgcgacggg atttttgatt tcaacgtcgg ttatatcgcc
     3061 agtgcggttt tggctttggt tttccttgca ctgggcgcgt ttgtgcaata cggcaacggc
     3121 gaagcagtgc agatggcggg cggcaaatat atcgggcaat tgatcaatat gtacgccgtt
     3181 accatcggcg gctggtcgcg cccgctggtg gcgtttatcg cttttgcctg catgtacggc
     3241 acgacgatta ccgttgtgga cggctatgcc cgtgccattg ccgaacccgt gcgcctgctg
     3301 cgcggaaaag acaaaacggg caacgccgaa ttctttgcct ggaatatttg ggtggcgggc
     3361 agcggtttgg cggtgatttt ttggtttgac ggcgtaatgg cgaatctgct caaatttgcg
     3421 atgattgccg cttttgtgtc cgcccctgtg tttgcctggc tgaattaccg tttggtcaaa
     3481 ggtgatgaaa aacacaaact cacatcaggt atgaatgccc ttgcattggc aggcttgatt
     3541 tatctgaccg gctttaccgt tttgttccta ttgaatttgg cgggaatgtt caaatgataa
     3601 cgatgccgtc tgaaaccgca aaccgctttc agacggcatt gccgcgttta tggaaaaaaa
     3661 caccgacatc gcgtaaaata tgcgcaaatt ttgtgattcg gtcaggtcgt ctgaaacaga
     3721 ttgcgcctga tttatttttt cggaaaaccc tatgagcgaa caaaaccatc cgcaaaccga
     3781 gccgcagttg gacgaaaacc aaatcatcgc cctgcgccgc gaaaaactgc acaacatccg
     3841 ccaacagcgc aacgcctacc ctaacgattt caaacgcgac agctttgccg ccgacctgca
     3901 tacccaatac ggtgaaatcg gcaaagaaga actcgacccg caaggcattc ccgtcaaaat
     3961 tgccggccgc atgatgctca aacggcaaat gggcaaggcg agctttgcca ccattcaaga
     4021 cgtgtccggg caaatccagc tttatctgaa caacaaaggc gtgagccaag aaattttgga
     4081 cgacttcaac cattgggact tgggcgacat cgtcggtgcg gaaggcactt tgttcaaaac
     4141 caaccacggc gaactgaccg tacgcgtgtc cggcatccgc ctgctgtcca aatccctgcg
     4201 tccgctgccc gacaaacaca aaggtttgag cgatcaggaa accaaatacc gccaacgcta
     4261 tgttgatttg attgccaacg gagaatcgcg caataccttc atcaaacgca gccaaatcat
     4321 ccaatccgtg cgtaatttta tggtgggcga gcattatctc gaagtcgaaa ccccgatgat
     4381 gcacccgatt cccggcggcg cgacggcaaa acccttcgtt acccaccaca atgccttgga
     4441 cattccgctc tacctgcgca tcgcccccga gctgtatctg aaacgcttgg ttgtcggcgg
     4501 tttggagcgc gtgtttgaaa tcaatcgcag cttccgcaac gaaggtatgt ccgtgcgcca
     4561 caatcccgaa ttcaccatga tcgaattcta cgaagccttc tccgactacg aacgtatgat
     4621 gcagatggca gaagacatca tccgcaacgc atcgcgcaca gtaaacggta cggcaaacat
     4681 cacttacaat ggcaaagaag tcgatttgga aagcccgttc gagcgtctga ccatcctcga
     4741 agccatcaaa aaatacaatc cgcactacac cgacgagcag ttgaacgatg cggaatggct
     4801 gaaaaaagaa atcgtcaaac acggtgaaag cctgccgccg tccccgggca tcggcagcct
     4861 gcaactcgcg ctgtttgaag gttgcgccga gggcaagctg tggaacccga ccttcatcat
     4921 cgattacccg gtcgaagttt ccccgttggc gcgcgcttcg gataccaaac aaggtctgac
     4981 cgaacgtttc gaattgttcg ttgtcggccg cgaactggca aacggctatt cagaattgaa
     5041 tgaccctgaa gaccaagccg aacgcttcaa atcgcaagtc gcacaaaaag acgcgggcga
     5101 cgacgaagcc atgcattacg atgccgacta catccgcgcg atggaatttg gtttgccgcc
     5161 gaccggcggt tgcggcatcg gtatcgaccg cttggtgatg ttgctgaccg attcgcaaac
     5221 catccgcgat gtgattctgt ttccgcagat gcgccccgag tagtcataaa aacagttgaa
     5281 atggcaatgc cgtctgaacc cgattggatt cagacggcat tttgtatggc ggtacggatt
     5341 tattcgcttt ctaactgacc gacccattcg gacaaggcag tcaggcgttg ttcggatttc
     5401 gccacgaacg ggttttcggt atcccacgcg tagcctgcca aaatcgaaga aagcatacgg
     5461 ctgatttccg ggctgcggtc gggcgcgtac acgacgtttt ggaagcggaa atcgtaccaa
     5521 ccgtccacac atcaggggtg gtaatccgaa tcaacacgcc ttctcgcctg tgcggcggac
     5581 ataaaccgtt ttttgacctc cagcaccaca cctaaaatcg tgctgccttc gggcaggtca
     5641 aaggcttcag acggcctgcc cgtctgataa atcagcatcc tgcccgcaat atcgataaca
     5701 aggtgcgcga cgaccagccg cttgtcggac agttcaatca gcacgatgtc ctcgtccgca
     5761 gggcgcatac gcggctcgac caccaccaaa tccccttttt cgataaccgg cgacatggac
     5821 gtgtcgggca tggcaacggc aaacgtgtac tgcgaggggg cgaccaccga agcaaacaag
     5881 tcgaaactgc ctgtgagttt gcctgtacgg gcgaaagccg ccgcctgacg gtctgacaac
     5941 agggggcgtg gtgtgagcct catcgacagt gctttgccgt cggcatcgct gtggttgtgc
     6001 gtatgcgtat gatggttgtt gatggtcgta tcgccttgga catcgttaag gactatgctg
     6061 tttgaacctg ccacggattt ggtcatctca ttgtaggctt gcgtcgtgat gcgctcttgc
     6121 ttggtcatac ctgttacagc ctgtttcagc atttctaacg ctatgtgatt cttcctaagg
     6181 gtcgctatac cggcttttgc agcttgctca ttaccacaag aggggcaacc ccagctactt
     6241 cctatgaaca tattggagta cctcgaacag gtttggtttc catgtttagg gcagttgatt
     6301 acacacggct tggctgtacc ctcaaactcg accaacttaa tgccacgatg accgaaacgt
     6361 tcctcaacct cagcagcagc ttcttcaaat gtttttgacc ttcttgccat attgacctcc
     6421 ggattattga ttattttggc tatgctttaa cgagggattg gaatga
//
LOCUS       DAXHTR010000120         6461 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.120, whole genome shotgun sequence.
ACCESSION   DAXHTR010000120 DAXHTR010000000
VERSION     DAXHTR010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6461
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.120"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            150..1691
                     /locus_tag="ACFNML_001641"
     CDS             150..1691
                     /locus_tag="ACFNML_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFC6392988.1"
                     /translation="MDGRRWVVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFLDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLSAFALGIFLLLDNGEGGK
                     QTETL"
     gene            1720..2319
                     /locus_tag="ACFNML_001642"
     CDS             1720..2319
                     /locus_tag="ACFNML_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class carbonic anhydrase"
                     /protein_id="HFC6392989.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            2316..2909
                     /gene="nadD"
                     /locus_tag="ACFNML_001643"
     CDS             2316..2909
                     /gene="nadD"
                     /locus_tag="ACFNML_001643"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFC6392990.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPAGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVREGATYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGKSLQDGSVRIL
                     SAPMHNVSSTEIRRAGVSDGIPPAAARYIREHGLYEK"
     gene            2968..3354
                     /gene="rsfS"
                     /locus_tag="ACFNML_001644"
     CDS             2968..3354
                     /gene="rsfS"
                     /locus_tag="ACFNML_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFC6392991.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            3408..3878
                     /gene="rlmH"
                     /locus_tag="ACFNML_001645"
     CDS             3408..3878
                     /gene="rlmH"
                     /locus_tag="ACFNML_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFC6392992.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARMMMRLSSLTLPHGMVRVFLTEQLYRAVSILHNHPYHRE"
     gene            3951..4217
                     /locus_tag="ACFNML_001646"
     CDS             3951..4217
                     /locus_tag="ACFNML_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFC6392993.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            4374..5057
                     /locus_tag="ACFNML_001647"
     CDS             4374..5057
                     /locus_tag="ACFNML_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFC6392994.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPASAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            5181..5693
                     /gene="coaD"
                     /locus_tag="ACFNML_001648"
     CDS             5181..5693
                     /gene="coaD"
                     /locus_tag="ACFNML_001648"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFC6392995.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            5725..6378
                     /locus_tag="ACFNML_001649"
     CDS             5725..6378
                     /locus_tag="ACFNML_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HFC6392996.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRCGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWKIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
BASE COUNT         1402 a         1701 c         1858 g         1500 t
ORIGIN      
        1 gcttcgtcgc cttgtcctga tttttgttaa tccactatat ttcagacggc gggaggaagg
       61 cggatacggt atgtagtcgg attgaaatat acggtatgtg ggggcttttg ggtacaatcg
      121 caacatcagt gtttcaagtg gggaaggcga tggatggacg gcgttgggtg gtatggggtg
      181 cttttgccct gctgccttcg gcttttttgg cggtaatggt cgttgcgcct ttgtgggcgg
      241 tggcggcgta tgacggtttg gcgtggcgcg cggtgctgtc ggatgcctat atgctcaaac
      301 gtttggcgtg gacggtattt caggcagcgg caacctgtgt gctggtgctg cctttgggcg
      361 tgcctgtcgc gtgggtgctg gcgcggctgg cgtttccggg gcgggctttg gtgctgcgcc
      421 tgctgatgct gccttttgtg atgcccacgt tggtggcggg cgtgggcgtg ctggccctgt
      481 tcggggcgga cgggctgttg tggcgcggca ggcaggatac gccgtatctg ttgttgtacg
      541 gcaatgtgtt tttcaacctt cctgtgttgg tcagggcggc gtatcagggg tttgtgcaag
      601 tgcctgcggc acggcttcag acggcacgga cgttgggcgc gggggcgtgg cggcggtttt
      661 tggacattga aatgcccgtt ttgcgcccgt ggcttgccgg cggcgtgtgc cttgtcttcc
      721 tgtattgttt ttcggggttc gggctggcat tgctgctggg cggcagccgt tatgccacgg
      781 tcgaagtgga aatttaccag ttggtcatgt tcgaactcga tatggcggtt gcttcggtgc
      841 tggtgtggct ggtgttgggg gtaacggcgg cggcagggtt gctgtatgcg tggttcggca
      901 ggcgcgcggt ttcggataag gcggtttccc ctgtgatgcc gtcgccgccg cagtcggtcg
      961 gggaatatgt gctgctggcg tttgcggcgg cggtgttgtc tgtgtgctgc ctgtttcctt
     1021 tgttggcaat tgttgtgaaa gcgtggtcgg ccggcgaatc gtggcgtgtg ttaatggaaa
     1081 gtgaaacgtg gcaggcggtg tggaatactt tgcgcttctc ggcggcggcg gtgtatgcgg
     1141 cggcggtttt gggtgtggtg tatgcggcgg cggcgcggcg gtcggcgtgg atgcgcgggc
     1201 tgatgttttt gccgtttatg gtgtcgccgg tttgtgtttc ggcgggcgtg ctgctgcttt
     1261 atccgcagtg gacggcttcg ttgccgctgc tgctggcgat gtatgcgctg ctggcgtatc
     1321 cgtttgtggc aaaagatgtt ttatcagcct gggatgcact gccgccggat tacggcaggg
     1381 cggcggcggg tttgggtgca aacggctttc agacggcatg ccgcatcacg ttccccctct
     1441 tgaaaccggc gttgcggcgc ggtctgactt tggcggcggc aacctgcgtg ggcgaatttg
     1501 cggcaacctt gttcctgtcg cgtcccgagt ggcagacgct gacgactttg atttatgcct
     1561 atttggggcg tgcgggtgag gataattatg cgcgggcaat ggtgttgaca ttgctgttgt
     1621 cggcgttcgc gctgggcatt ttcctgctgt tggacaacgg cgaaggcgga aaacagacgg
     1681 aaacgttata atgtgaaccc tttttcagag gacggcaaaa tgagcgagtt ggataacatc
     1741 cttgcccata accggcagtt tgtcgagtcg ggcgaatatg aaaaatactt taccgacaaa
     1801 taccccgaac gcgggctggc agttttgtcc tgtatggatg cgcggattat cgggctgctg
     1861 cccgacgcgt tgggtttgaa aaacggcgat gccaagctga ttaaaaatgc cggcgcgctg
     1921 gttacgcacc cgtggggttc ggtgatgcgg agtcttttgg ttgccgtgtt tgaattgaag
     1981 gtcagagaga ttatggtcat cgcccatcac gattgcggta tgcaggggct gaatgccgaa
     2041 gaattcctcg ggcgcgtccg ggaaagccag attcccgaag accgtatcga aaccctgcgt
     2101 tatgccggta tcgacctcga cggctggctg accggtttcg acaacgtcga agacagcgtg
     2161 cgccacacgg tggaccttat ccgtaaccat ccgctgatgc cgcgccatat cgccgttcac
     2221 ggactggtca tccatcccgt taccggcaaa ctgacgctgg ttgtggacgg cagtgtttca
     2281 gacggcatgg acttatcgga aggaatggaa acatcatgaa gaaaatcgga ctgttcggcg
     2341 gcactttcga cccgatacac aacgggcatc tccacatcgc ccgcgccttt gccgacgaaa
     2401 tcggcttgga cgcggttgtt ttcctgccgg caggcggccc gtatcacaaa gacgcagcct
     2461 ccgcttccgc cgccgaccgc cttgccatgg tcgaactggc gacggcagaa gacgcgcgtt
     2521 ttgccgtcag cgattgcgac atcgtccgag aaggtgcaac ctatactttt gataccgtcc
     2581 aaatcttccg ccagcagttc ccatccgcgc aactctggtg gctgatgggc agcgacagcc
     2641 tgatgaagct gcacacatgg aaaaaatggc agatgctcgt gcgcgaaacc aatatcgccg
     2701 tcgccatgcg gcagggcgac agcctgcacc aaaccccgcg cgaactgcac gcgtggctgg
     2761 gcaagtccct tcaggacggc agcgtccgca tcttgtccgc cccgatgcat aatgtgtcgt
     2821 caacggaaat ccgccgcgcc ggtgtttcag acggcatccc acctgccgcc gcacgctaca
     2881 tccgcgaaca cggtttgtat gaaaaataaa gtcaaatcag taagaaacgg ctataatgcc
     2941 gtctgaaaac atcccgttag gaaaataatg aacgaacaag aactgcaaga cctgcaaaaa
     3001 atggtcgggg tcgccgtcaa cgccctcgaa gacatcaaag ccaaagacat ttccgttctc
     3061 gaaacgcaag acaaaacttc gctgtttgcc agaatgatta tcgccagcgg cgacagtacg
     3121 cgccaagtca aagcactggc caacaacgtt gccgtcgatt tgaaagaagc cggttttgaa
     3181 atcctcagta ccgaaggaga cagcggcgaa tggacgctcg ttgatgcagg agacctcgtc
     3241 gtccacgtta tgctccccgc cgtgcgcgac ttctatgata tcgacaccct ttggggcggc
     3301 gagaaaccga gttttcacgc cggaatgcag aagccgtggc acgcggcaga ctgattcccg
     3361 atgccgtctg aatgttcaga cggcattttc ctgtaaggga gaaagcattg aacattaccg
     3421 ttttggcagt cggcaccaaa atgccgcgct gggttgatga ggccgtcgcc gaatacgcca
     3481 aacgcttcgg acgcgacgtc gcctacgcac tcaaagaaat caaacccgaa aaacgcggcg
     3541 cgggcgtgaa tgccgcccaa ggtatggcgg cggaagaaaa acgcatcctc gaagccgttc
     3601 cgcaaggcgc gttcctcgtc gttcttgacg aacgcggcaa agcgccgacc tccgtcgagc
     3661 tggcggaaca cctcaaaagc tggcggcaaa acggcgaaca cgtctgcttc gtcatcggcg
     3721 gcgcggacgg catgaccgac cgcctcaaac aacaagcccg catgatgatg cgcctgtcca
     3781 gcctcaccct gccgcacggc atggtgcgcg tctttctgac cgagcagctc taccgcgccg
     3841 tttccatcct gcacaaccat ccctatcatc gggaataaga aggcttttgt ccgtatccct
     3901 aatcggttaa aatcaccccg ttatttctga acacagcaaa ggaatccgct atggcacgca
     3961 tggtattctg cgtcaagctc aacaaagaag ccgaaggcat gaaatttccg ccgcttccca
     4021 acgaattggg caagcgcatt tttgaaaacg tatcgcaaga agcgtgggcg gcgtggacgc
     4081 gccaccaaac gatgctgatt aacgaaaacc gtttaagcct cgccgatccg cgcgcgcgcg
     4141 aatacctggc tcagcagatg gagcagtatt tcttcggcga cggcgcggat gccgttcagg
     4201 gatacgttcc gcaataacgg ttttccgttt tgaacacagg ctgtccgaaa ctgcttcaga
     4261 cggcctttaa aatacgccgg acaactttat cttcacgcaa ccggacaaat tttgacattg
     4321 ggcaatgtta aaatccccca ttattcccaa ccgcgctttc aggagcagat gatatgcaac
     4381 acgacgttta cgactacacc gcgcatacgg tttctaaaaa caccgtcctg cagaaaacct
     4441 accgcctgct cggattttca tttattccgg cttccgcagg cgcggcactt gccgccaatg
     4501 ccggtttcaa tttttacgcc gccttcggtt cgcgctggat agggtttgcc gtcgtgttgg
     4561 cgtttttcta cggtatgatc cacttcatcg agaaaaaccg ttacagcaat accggcgtta
     4621 ccctgctgat ggtattcaca ttcggtatgg gcgtattgat cggccccgtg ctgcaatacg
     4681 cgctccatat tgccgacggt gcgaaaatcg tcggcattgc cgccgcgatg accgccgccg
     4741 tctttttaac gatgtccgcc ttggcgcgcc gaacccggct cgatatgaac gcgctcggac
     4801 gcttcctgac cgtaggtgcg gtcattctga tggtcgccgt ggttgccaat ctgtttttgg
     4861 gtattcccgc actcgccctg accatttccg ccggttttgt cttgttcagt tccttaatga
     4921 ttatgtggca ggtacgcacc gtcatcgacg gcggcgaaga cagccacatc agcgcggcac
     4981 tgacactgtt tatctcgctt tacaacatct tcagcagcct gctgaacatc ctgttgtctt
     5041 taaacggcga ggattgatgc cggtaaaacg ccgtccgact atgccgtctg aaactgcttc
     5101 agacggcatt ttactttggg catacaatac ggatgcacac gcaccaacac acagccgact
     5161 atgaagccgg gaggaatcaa atgttaccga atacaccgcg ccgcgccgtt tatgccggca
     5221 gtttcgatcc gcccacattg gggcatctgt ggatgatacg gcaggcgcaa tctatgtttg
     5281 acgaactcat cgtcgccatc ggcattaacc ccgacaaacg cagcacctat accgtcgctg
     5341 aaaggcagga tatgttgtgc gatattacta aaatgtttcc caacgtcaga accgatgtat
     5401 ttgaaaaccg atttttggtg cattacgccc gtgaggtaga tgcaggattc atcgtgcgcg
     5461 gcatccgttc tgcttcggat tacgaatacg aacgttccat gcgccatatc aacagcgacc
     5521 tcgcccccga aatatccacc gtattcctca tgccgccgcg cgaaatcgcc gaagtgtcgt
     5581 ccactatggt caaaggactg gtcgggccgg aaggctggac ggaaacgatc caccgctacg
     5641 tgccgcaagc tgtgtacgaa aaaatccttg ccgaacatca acacgaaaat tgaacccttc
     5701 tatttttgca aggcatatat ggatatgttg gaaattttgt tccgccatca agacttcgtc
     5761 gccatcaaca aaccgggcgg catatccgtc caccaagaca gcggcgagac cggactggcc
     5821 cgaacactcg ccatacagtt gggcgtagag cgtgtgtggc tgctgcaccg attggacaaa
     5881 cagaccagcg gcattttact gtttgccctc aacaaagaaa gcgcgtccgc ccttagcgga
     5941 cagtttgccg gcaaaagcat caaaaaaacc tatttggcac tgtccgaccg caagccgtcc
     6001 aaaaagcagg gttggattaa aggcggaatg gaaaaatcca gatgtggaat gtggaagttg
     6061 acgcgcaata cagaaaatat cgccgttacc cgattccaca gcatcagtat cgccgaaaaa
     6121 ctgcggctgt tcatcctaga accgcatacg ggcaaaacgc accaattgag agtggcgatg
     6181 aaaagtttgg gcagtccgat tctgggcgac agcttatacg gcggaacaga atccgaaacc
     6241 atgtttctgt atgcatggaa aatacaattt gcctaccaaa accgacaaat cgaaattgtt
     6301 gcacccttaa aaaacgaatg gcagactgaa aatatctctc atgcgctgga agaattttat
     6361 atggcaaaag caggttaata aatataaatt aatttataaa caatatgata aatattttta
     6421 taaaaatttt tcaagaaaac ggttgactgc aaatctcttc a
//
LOCUS       DAXHTR010000121         6382 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.121, whole genome shotgun sequence.
ACCESSION   DAXHTR010000121 DAXHTR010000000
VERSION     DAXHTR010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.121"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            176..1234
                     /gene="alr"
                     /locus_tag="ACFNML_001650"
     CDS             176..1234
                     /gene="alr"
                     /locus_tag="ACFNML_001650"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFC6392997.1"
                     /translation="MRPLNVQIRLGNLRHNYRILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGSDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1298..2653)
                     /locus_tag="ACFNML_001651"
     CDS             complement(1298..2653)
                     /locus_tag="ACFNML_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HFC6392998.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISSDINVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(2668..2940)
                     /locus_tag="ACFNML_001652"
     CDS             complement(2668..2940)
                     /locus_tag="ACFNML_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HFC6392999.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENRINILNISQQLMDDFFT
                     MIILVDTSKCPKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3066..3803)
                     /locus_tag="ACFNML_001653"
     CDS             complement(3066..3803)
                     /locus_tag="ACFNML_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HFC6393000.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="ACFNML_001654"
     CDS             complement(4084..4983)
                     /gene="prmB"
                     /locus_tag="ACFNML_001654"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFC6393001.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGDGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5128..5721
                     /locus_tag="ACFNML_001655"
     CDS             5128..5721
                     /locus_tag="ACFNML_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393002.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIMTKLDDLLRYAKYEGSYSDRKGHPNIIAAH
                     KYRAVAKVGNESLNIGVIVREFPDDHKHYDHFILKDE"
     gene            5833..>6382
                     /locus_tag="ACFNML_001656"
     CDS             5833..>6382
                     /locus_tag="ACFNML_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFC6393003.1"
                     /translation="MTKLYAEIAKMETQDDDTVKVWGYASSEEIDSDGEVIATAAMKA
                     AIPDYMKFGEGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVAKVKTGVYKGFS
                     IGGSVTARDDLNKSQITGLKLTEISLVDRPANPDAVFTCFKADKGAEAVNNDTEHNAT
                     YFSHFPSKQKSTGGSRCPFLYRF"
BASE COUNT         1742 a         1603 c         1467 g         1570 t
ORIGIN      
        1 atttgggaaa gcgattatat caggaaaagc aaaccgcctt cctacctgaa aactgctgct
       61 tcggcttgaa gacacaaggg tctttaatat tttaaaagcc ttgccgttgg attataattc
      121 cctaattgat ttcttaattt tgctaataaa cacttgcttg gtaaggaatg aatttatgcg
      181 ccctttgaac gtgcagatca ggttgggcaa ccttaggcac aattatcgga ttttaaagga
      241 aatgcacgga ggcaagctgt tggcggtagt gaaggccgac gcatacggac acggtgcggt
      301 cagatgtgct ttcgcgctgg cagacttggc agacggcttt gccgtggcga caatcgatga
      361 aggaatcagg ctgcgggaga gcggcattac ccatccgatt gtccttttgg aaggcgtatt
      421 tgaagcatca gagtacgaag cggtcgaaca atactcgctc tggccggcag tcggaaacca
      481 atggcaactt gaggctttgc tttcccgcca ttggaaaaaa cctgtcaagg tctggttgaa
      541 aatggattcg ggaatgcacc gtaccggatt tttccctcat gattacgctt cggcatatgc
      601 ggcattgaaa cagtcggaat atgtggacag tattgtcaaa ttctcgcatt tctcctgtgc
      661 ggacgaaccc gaaagcggta tgacggaaat acagatggaa gcattcgatt tgggtacgga
      721 agggctggaa ggcgaagaaa gccttgccaa ttcggcggct attttgaatg ttcccgaagc
      781 acgcagggat tgggggcgcg cgggcttggc gttatacggc atttccccgt tcggaggcag
      841 cgatgacagg ctgaaacccg tgatgaggct ttcaacccgt atttttggcg aacgcgtttt
      901 acagccgcac tcccctatcg gttatggcgc aacattttat accagcaaat ctacgcgcgt
      961 cggtctgatt gcctgcggtt atgcggacgg ttatccgcgc cgcgctccaa gcaattcccc
     1021 cgtcgccgtc gacggtaaat tgacccgggt catcggcagg gtctctatgg atatgatgac
     1081 catcgagctg gacgcttcgc aagaaggttt gggacacgag gtcgaactgt ggggcgatac
     1141 ggtcaacatc aatactgttg ccgaagcggc cggaaccatc ccttacgaat tgatgtgcaa
     1201 tatcaaacgt gcaaaattca cttatatcga gtaatcgagt ccaaacaaaa atgccgtctg
     1261 aagcctttca gacggcattt ccccatcaaa accgcaatca gtttttcatt gattgaaccg
     1321 gagccgggat tctgccgccc cgtttgacga acacttcgca cgaaccttct ttgaccggca
     1381 ttacaggcgc gtagcctaac aggccgccga actcgacact gtcgccgacg gttttaccgg
     1441 ttaccggaat aatgcgcacg gcagtggttt tgctgttgat catgccgatg gcggcttcgt
     1501 cggcaatgat gccggaaatg gtgtgcgcgg gtgtgtcgcc gggaacggca atcatatcca
     1561 agccgaccga acaaacggcg gtcatcgctt cgagtttatc caacgtcagc acgcctgctt
     1621 cggcggcggc aatcatacct tcgtcttcgg aaacggggat aaacgcgcca ctcaaaccgc
     1681 caaccgcgct cgaagccatc atgccgccct ttttcacggc atcgttcagc aatgccaaag
     1741 ctgctgttgt gccgtgcgta ccgcagacgc tcaaacccat ttcttcaaga atgcgcgcca
     1801 ccgagtcgcc gacggcaggg gtcggtgcca gcgacaagtc gagaatacca aacgggatat
     1861 tcagcatttt tgaggcttcg cggccgatga gttcgcccac gcgggtaatt ttgaaggcgg
     1921 ttttcttcac aacttcggca acttcggtca atgtcgttgc atccgaattt tccaacgcgg
     1981 cttttacgac acctgggccg gatacgccga cattaatcac agcatccgct tcgcccgatc
     2041 cgtgaaacgc acccgccata aacggattgt cttccaccgc attgcagaac acgacgattt
     2101 tcgcgcagcc gaaaccttcg ggcgtaattt cagccgtgcg tttgatggtt tcgcctgcca
     2161 gcttgaccgc atccatattg ataccggcgc gcgtactgcc gacattgatg gagctgcaca
     2221 cgatgtcggt ggtcttcatc gcttcgggaa tggaacggat taacacctcg tctgaaggcg
     2281 acatgccttt ttgcaccaac gcggaaaagc cgccgataaa ggacacgccg atggctttgg
     2341 cagccttatc caaagtttgc gccacgctga cataggaatc ggcatgggta gccgccgcga
     2401 tttgggcaat cggcgtaacg gaaatgcgct gattcacaat cggcacgccg tatttggcag
     2461 acagatattt tgccgtcgcc accaagtctt tgccgaccgt ggtaattttg ttgtaaatgt
     2521 tttggttcaa cacattgata tcgctgctga tgcagtcgtg cagatcaatg ccgatggtaa
     2581 tggtgcggac atcaaaattc tggtcggcaa ccattttgac ggtttctaaa atttcgccgg
     2641 attggatact catcacattc ctccgactca aatgcggtgc atcgcttgga agatttcttc
     2701 attttgcata cggatatcaa gcgcgagttt tttgctctct tccgcaaaca aatccaaaac
     2761 ctcctgccga gatttggggc atttcgaggt atccaccaag ataatcatgg taaaaaaatc
     2821 atccatcagc tgttggctga tattgaggat gttgattcgg ttttccgcca aaattttgga
     2881 aacatcgtac acgatgccga cgcggtcttt accgatgacg gtgatgactg aattgttcac
     2941 aggcttactc cttgcagata tccgttaaag tccgaaatta taccaccgtt ggattttgaa
     3001 gaaatattgt caacaatata tacatacaaa atgccgtctg aaactatttc agacagcatc
     3061 aagattcagg gttcgattaa ataaccatcc ttatcccact gggttttcct gaccaacttg
     3121 tcatcctgat aaacagcttc actcttttta gaaccatctt cataccactc taagactact
     3181 ccattacgtt tatgatgatg gatagacagt tccgagagta atcgaccgct ttcatcccat
     3241 gtcagaatct tggccggctc atcattgacc ataaccattt ccgttttgat attgccatca
     3301 gcataccatt gtttccatac gccgtttgcc ttattttgtt taaactggat ttcgctttcc
     3361 ttgctgccat tacggtaata gcggtacccc gtaccttcac tcaaaccatt tttataaggc
     3421 ataaccgcag attttttacc gttcggatac cagttgaccc actcaccgtc cggcttaccc
     3481 ttgctgaagc cccccgccat ttttttctga ccattaaaat gccacaaaat caacataccg
     3541 ttttgcaggg taggcacaaa agatttgatt tgcgttgaag caacgatata aggttcggaa
     3601 tatttcttca tcgacggata ataaaaatcc tgcgcgtacg caataccctc cgccacacta
     3661 tattgcctga tataagcggc agaagacatc gtcgccgtca gtttcccgtt ctgattaaaa
     3721 taaacagaat aggtctgtgc cgataaagaa gccgaaaaac ccaacaggac aattgaaaat
     3781 acaatccgag ataatttttt cattgcaata gcgatataaa aacaaggctg tgttttagta
     3841 atctgttgat ttcaattatt tgcaagggaa aagacaatta ttttccggtt aggaataaac
     3901 ctattctact gaatatattg aagccaagta cgcctatcaa cactatatta aaacactgcc
     3961 aaaaacaatt aacttataaa caatatggta aggatttctc tgccaagcat caaacccgag
     4021 acaacgtatc gtaaaaatgc cgtctgaaaa caaatcgtct tcagacggca tttccccttc
     4081 aactcactct tcacccaata actgctcgcg cgtcaagagg aaaacaaaac cgtcgccccc
     4141 gctggtttcc aaccaagtaa aaggcaactc cggatacgct gcttccaata catccctgtt
     4201 atgcccgatt tccaccagca atacaccttt gggattcaga aactttgccg cattcagaag
     4261 aatctgcctg gtggcatcca acccgtccgc cccgctgccc aatgccaatt ccggttcgtg
     4321 caaatactct tcaggcaata actcaaccga ttccgcatcc acataaggcg gattggaaac
     4381 aatcaaatca taagtgcctt ccaatccttc aaacaaatcc gtatgaataa gccggatgcg
     4441 ttcttccaaa ccataatctt cgacattaat ccctgccact tccaaagcat ccaagctcac
     4501 atcaaccgca tcaatttggg catcaggata atgatgcgcc atctgaatgg caaggcaacc
     4561 gcttccggtg caaagatcca aagcattatg caccaactca tcgtattcta tccaaggacg
     4621 aagtccgtca cccaacaatt cataaataaa agaacgaggt atgattacgc gctcatccac
     4681 atagaaatca aactctccct gccatgcctg gtgtgtcaaa taagcggctg gaatgtgttc
     4741 gacagcacga cgctcaataa ccgtcagcac ttcctctttt tcagcttcca agagttttgc
     4801 atcaagatat ggggcaagca tatccaaagg caaattcaaa gtatgcagaa tcaaataagc
     4861 tgcttcatca tgcgcattat ccgttccatg accaaaaaag agccctgcct cattaaaacg
     4921 gctgactgca aaacgtaaaa catcgcggat agtcgtcaat tcttgtgctg cctgattaaa
     4981 cataatatga accattctgc gtatagatac ttttaattat aacagaaaca acaagcaaac
     5041 cttttcatat cgccaaataa ccacccaatc tacccataca actacataaa tgcccgcgcg
     5101 aaaaccatcg cccgaacgga aacgacaatg gccgacggta tgggcaacct gattggctgg
     5161 gaaaaaacgg ggcttgttgc cggtaagcag tggataaccg caaaagacga caaggtgtcc
     5221 gatgtctgca atgccaacgg cgagatgggc gtaatcgggc tttacgagcc tttctcacac
     5281 ggcgcattga cgatacccgg tcatccgaac tgccgatgcg aggttgtttc cgtatcgggt
     5341 ggcgaattgg gggaatttgc cgaaaaaaag gagcttcgta aagcggctat gcagtatgcg
     5401 cgggataact ttatcggcaa aagctatgtc aatgaaaaca gcgggcatga actgaaggta
     5461 acttggcaag gtgtgaaaca cgctgcgtca aaggcaaatc aggcggaatt atccatcatg
     5521 acaaaacttg atgacttatt gcgctacgca aaatatgagg gttcttattc ggataggaaa
     5581 ggtcatccta atattattgc agcacataag tatcgtgccg ttgccaaggt tgggaatgag
     5641 tctttaaata tcggtgtgat tgtaagggaa tttccagacg accataaaca ttacgaccat
     5701 ttcatcttga aggatgaata aagccctttt gcagtgtcgt tctggagcgg atagcgttaa
     5761 ggcaagtaca cttccagcct tgaaaaaggg ctttaaattc agcatgccat ttatacaggc
     5821 aggagtaaac ccatgacaaa gttatacgca gaaatcgcca agatggagac gcaggacgac
     5881 gacacggtca aggtttgggg ttacgcttca agcgaggaaa tcgattcgga cggcgaagtc
     5941 atcgcgacgg cagctatgaa ggcggcgatt cccgattata tgaagtttgg cgaggggcgc
     6001 gagatgcacg gctcaaacgc tgcgggaacg gcaattgaaa tcaacgttga agacgacggc
     6061 ataacctttt tcggtgctca tatcatcgac cccgttgtcg tggcgaaagt caaaacaggc
     6121 gtttacaaag gcttttccat cggcggcagc gttaccgccc gcgatgattt gaacaagtcc
     6181 caaatcacgg gcttgaagct gacggaaatc agccttgtcg accgccctgc caatcccgat
     6241 gcggtgttta cctgctttaa ggcggacaaa ggtgcggaag cggtaaacaa cgatacagaa
     6301 cataatgcta catattttag ccatttccct tccaaacaaa aaagcaccgg cggcagccga
     6361 tgccccttcc tttacaggtt cc
//
LOCUS       DAXHTR010000122         6170 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.122, whole genome shotgun sequence.
ACCESSION   DAXHTR010000122 DAXHTR010000000
VERSION     DAXHTR010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.122"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            239..685
                     /gene="yciA"
                     /locus_tag="ACFNML_001657"
     CDS             239..685
                     /gene="yciA"
                     /locus_tag="ACFNML_001657"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFC6393004.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKIEVWVKTL
                     MNDCVTEDRYLVTEAVFTYVAIDAEGNPRPIPKEGNPKLSGLLPTP"
     gene            complement(736..1557)
                     /locus_tag="ACFNML_001658"
     CDS             complement(736..1557)
                     /locus_tag="ACFNML_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFC6393005.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQVLADEL
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLTFLTRKILPQMVERGGGYVMNLGSIAGNYAYAGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGDTEFSNVRFKGDDERAAGVYESVEFIRPEDIAETSLW
                     LYRRPAHMNVNTIEIMPVAQTFAGMKVIKKAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            1676..2134
                     /locus_tag="ACFNML_001659"
     CDS             1676..2134
                     /locus_tag="ACFNML_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HFC6393006.1"
                     /translation="MKTQISLAAAAITLLLSACGGSGIPSQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFESDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2298..3413)
                     /gene="lst"
                     /locus_tag="ACFNML_001660"
     CDS             complement(2298..3413)
                     /gene="lst"
                     /locus_tag="ACFNML_001660"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HFC6393007.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNQGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFHLPYGLNKSFNFIPTMAELKVKAMLLPKVKRIYLASLEKVSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKNFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPKDYWLKPVYALFTQSGIPILTFDDKD"
     gene            3640..3780
                     /locus_tag="ACFNML_001661"
     CDS             3640..3780
                     /locus_tag="ACFNML_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/arginine repetitive matrix 2"
                     /protein_id="HFC6393008.1"
                     /translation="MPPHIRHSTSDTAAKSGKTISPFLSRRRVQTRQDRTKREQSRQN
                     MP"
     gene            3847..6072
                     /locus_tag="ACFNML_001662"
     CDS             3847..6072
                     /locus_tag="ACFNML_001662"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFC6393009.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTADIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELRAPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGGKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTNGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     TKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1643 a         1806 c         1380 g         1341 t
ORIGIN      
        1 agattcccgc ctgcgcggga atgacggttc agttgcttag ggtaggattt ggcgggattg
       61 gtgggctgaa gcccacccta cggccccgcc ctacgcatcc gccctacaat gcgccttgcg
      121 aatctgccct acgcccgccc ttgtatgtct caaatgggtt aaactacgca cttcccgact
      181 tcccgtttca ggcaggggtg caggtccgat ttatatttca ataaaacaag gaaactttat
      241 gcagcacgaa gaaggcaacc gccaacgccc tcaaggcgaa ctgctcctgc gtaccgtcgc
      301 tatgccgcgc gataccaatc ccaaccaaga cattttcggc ggctggatta tgtcgcagat
      361 ggatttgggc ggcggcatat tggcggcgga aatcgcgcgg ggacgcatcg ttaccgtcgc
      421 cgttcaggaa atgaacttta tccgcccggt caaggtcggc aacgtcgtct gctgctacgg
      481 gcattgcgtc cgcgtgggca atacttccct ccagcttaaa atcgaagtct gggtgaaaac
      541 tttgatgaac gattgcgtta ccgaagaccg ctacctcgta accgaggcgg tgttcactta
      601 tgttgccatc gatgcggaag gcaatccgcg cccgattccg aaagaaggca accccaaatt
      661 gtcgggctta ttgcctactc cgtaaaaata cccgtaaaaa tgccgtctga aactgtcttc
      721 agacggcatt ccgcatcatt tgaaccagga cctgatgcgg gaaaacagcg acatactctg
      781 tttttcaaag tcttcccgca cttcgggcac ggcttttttt atcactttca ttcctgcaaa
      841 agtctgcgcc acgggcataa tttcaatcgt gttcacattc atatgcgccg gccggcggta
      901 cagccacaat gaggtttccg caatatcttc ggggcggata aattccacac tctcatacac
      961 gcccgccgcc ctctcgtcat cgcctttgaa gcgcacgttg gaaaactccg tatcgccgca
     1021 caaacccggc tcgatattgg taacgcggat atttttatcc gccaactccg cgcgcaaatt
     1081 caggctgaac tggcgcacaa acgccttggt cgccccgtaa acgttgctgc cggcataagc
     1141 ataattaccc gcaatcgaac ccaaattcat cacataaccg ccgccgcgtt ccaccatttg
     1201 cggcaaaatc ttgcgcgtca ggaacgtcaa acccaaaaca ttggtttgaa tcattgtttc
     1261 ccaatcttca aaatccgcct tgtccgccgt gtccaaaccc aaagccagcc cggcattgtt
     1321 gatgaggcag tcgatgtcgg aaaattcatc ggggatgccg tttaaggcat tttccaccga
     1381 ctcgcgtcgc gacacgtcca tttccaaagg gtaaaacaaa gcacccaatt catccgccaa
     1441 aacctgaagc ctgtccgcac ggcgcgccgc accgataacg cggtatccag ccccgacaaa
     1501 cgcgcggcac atcgcttcgc cgaatcctgc cgaagcaccg gtaattaaaa cagccattgt
     1561 ttttcctttc tttctcgttt cccaaacctg tttcaggtat cataacaccc ttcagacggc
     1621 atcgcgccga ctgaaaatga caacaaccat accaccacga caggacacat tcaatatgaa
     1681 aacccaaatc agccttgctg ctgccgccat cacactgctc ctttccgcct gcggaggcag
     1741 cggaatacct tcccaaccca aaggcgaaat ttccgaaaac cgcaccgccg cgttcaaatc
     1801 tatgatgccc gacttctcgc gtatgggcaa aatggtcaaa ggcgaagagc cttacgatgt
     1861 cgaaaaattc aaacaggctg ccgcggcgtt tgccgaaagc agcaagaaac cgttcacact
     1921 ttttgagtcc gatccgcaag gcaacggccg cgccctgcca gccgtttggt cggatggtgc
     1981 aaaatttgaa gccgaaaaaa caaaattcgc cgccgccgtc gaaaaactca atgccgcagc
     2041 acaaaccggc aaactggatg aaatcaaagc cgcctacggc gaaaccggcg caagctgcaa
     2101 atcctgccac gacagtttcc gcgcacccga ataacatccg aaacagaaca atgccgtctg
     2161 aagccttcag acggcatttt ctttgccatt tcagcacacg caaagcaatc agaaattatc
     2221 gtcaattcaa aaaatacgcc atatcctgat ttttgcctta cttactccgc cattttctat
     2281 tttgatttgc tatgcgatta atctttatcg tcaaatgtca aaatcgggat gccggattgg
     2341 gtaaacaggg catacaccgg cttgagccaa taatctttcg gaagggaagc cggtttcaat
     2401 gcgtaaacgt gcacattggg aaaatccttc atcgtcaacg ccgcgccgct gaaaaaggta
     2461 taaatttcat acctcgtatg cgggtttttc ttaatctcgc gcaaaatata gtcttcgatg
     2521 acatagggcg aatttaatgt ggttacgccg gaaagcccgt aggtttggcg ggggtgcggt
     2581 gcgacatatt gtatgttgaa gttttttgcc gccttttccg aaatttcctt catctccttg
     2641 tcgggcgaac ccaaaagtat ccgcaccgtg ccgcccgttt catcccccgc tttcagttcg
     2701 gacgcatcga acagcggcag gtaagtcatc ttgcggcggc cgtcgtccat aatgtttttc
     2761 aaacccttga atatcgtgta atgctcgtcg gaagcattac gggttttggc gatactccaa
     2821 tctccgatca tcatccgggc aaaattccgc ttgatcgtcc cgtttacaga aaactcatcg
     2881 cccaaatagc tgctgctttg aattaaattg cctgtcccgt cgtcaaaggt tttgatttcc
     2941 gcatccgggt aagtgctcaa aaaggcggca atgctgactt tttccaaact tgccaaataa
     3001 atccgcttga ccttcggcag cagcattgcc ttcaccttca gctccgccat cgtcggaatg
     3061 aaattaaacg atttgttcaa accgtagggc aggtggaaaa aatacgcccg ctccgcctta
     3121 tcctttatct gattgaaata ataatcgtat ttttcattcc tgttttcaga catcagcacc
     3181 acataaaacc gctcccccgg atgctgcgcc ataatccttt ccgccacctt catctgcaat
     3241 atggtatagc agaaaatcag attgaccggt tccccctctt cattgaagag tttgtccttc
     3301 agcagggaaa ccgcattcct ttccccctga tttacccggt caaatgtata aaatatcccg
     3361 aaacaaaaaa caatcagaca caacacggtc aaacaagcct ttttcaagcc catatcccta
     3421 aaactccatt ccgacaaatt aaacatacgc cccgagtgtc ccgggcggaa aaatgcggaa
     3481 ttttagcaga tattccgaca gttaccgaat attatagtgg tttaaattta aatcaggaca
     3541 aggcgacgaa gccgcagaca gtacaagcag tacgacaagg cgaggcaacg ctgtactggt
     3601 ttaaatttaa accactataa aatcaatttc accgaaaata tgccgccgca catcaggcac
     3661 agcacctccg acaccgccgc caaaagcggg aaaaccattt cccccttcct ttcacgccgc
     3721 cgcgttcaaa cacgccaaga tcgaacaaag cgcgaacaaa gccgccaaaa tatgccataa
     3781 tagccacgca cattccccgc gcaatgtgtt ccaaaagaaa aaaccaacct acaagagaat
     3841 aatcctatga ctcaaaaatc caccattgtt tatacccata ccgacgaagc ccccgcgctg
     3901 gcgacccaat cgctgctgcc gattgtgcag gcttttgccc gccacgccga tattgatgtc
     3961 aaaactgccg acatttccct gtccggccgt attttggcgg cattccccga atatctgacc
     4021 gaggcgcagc gcgtacccga cgcgcttgcc gaattgggcg aactggtaaa acaacccgat
     4081 gcaaacgtaa tcaaactgcc gaacatcagc gcatccgtac ctcaactgac cgccgcgatt
     4141 aaagaattgc agtctaaagg ctttgccgtt cctgactatc ctgccgaccc tcaaaccgat
     4201 gaagaaaaag ccgtacgcga acgctacgac cgcatcaaag gcagcgcggt aaaccctgtc
     4261 ctgcgtgaag gcaactccga ccgtcgcgcc cccaaagcgg tgaaaaactt tgccaaaaaa
     4321 aatccgcaca gcatgggcgc atggacaaaa gactccaaaa cccacgttgc caccatgcaa
     4381 agcggcgact ttttccataa cgaacaatcc gttaccgtac ctgaagcgac ttccgtatcc
     4441 atcgtgttca ccgacaaaca aggcaataaa aaagagctgc gcgcgcctgt tgccctgaaa
     4501 gccggcgaaa tcatcgacgc aaccgtgatg agcaaaaaag ccctgctcgc cttccttgcc
     4561 gaacaagtga aagacgcgaa ggcaaaaggc gtattgttct cactgcatat gaaagccacg
     4621 atgatgaaag tgtccgaccc gattatcttc ggacacgccg tcaaagtgtt cttcgcgcct
     4681 gtatttgaaa aattcggcgg caaactggct gccgcaggtg tcaacgtcaa caacggcttc
     4741 ggcaacctgc tggccaatct ggacaaactg gatgcggaca cccgcgccgc cgtcgaagct
     4801 gaaatcgccg ccgtttacgc cgccaaccct gatttggcga tggttgattc cgataaaggc
     4861 attaccaacc tgcacgttcc cagcgatgtc atcgttgatg cttctatgcc tgcgatgatt
     4921 cgtaactccg gccgtatgtg ggataaagac ggcaaagcgc aagacaccaa agccgtcatt
     4981 ccggacagca gctatgccgg tgtttaccaa gcaaccatcg acttctgccg cgaacacggc
     5041 gcattcgatc cgacaacaat gggtactgtg cccaatgtcg gtttgatggc gcaagcggcg
     5101 gaagaatacg gctcacacaa caaaaccttt gaaatcgaag ccgacggcca agtccaagtc
     5161 attgatgcgg caggaaaagt cctgatgcag catgacgttg aagcaggcga catttggcgt
     5221 atgtgccaaa ccaaagacgc tccggttaaa gactgggtgc aacttgccgt caaccgcgcc
     5281 cgtctgagca acacgcctgc cgtgttctgg ctcgacgaaa accgtccgca cgacaagagc
     5341 ctgctcgcca aggttaaagc ctaccttgcc gaactggata ccaatggcct cgacatccgc
     5401 gtcctcgctc ctgaagaagc cgccaagttc agcttgggtc gtctgaaaaa cggcgaagat
     5461 actatctccg taaccggtaa cgtcctgcgc gactacctga ccgacttgtt cccgattttg
     5521 gaactcggca caagcgcgaa aatgctgtct atcgttccat tgatgaacgg cggcggtatg
     5581 tttgaaaccg gcgcgggcgg ttctgcaccg aaacatgttc aacaattcct cgaagaaaac
     5641 cacctgcgtt gggactcttt gggcgaattt ctcgcactcg ccgtatcgtt tgaacatctg
     5701 gcgcaaaaaa ccggcaacac caaagcccaa gtcctcgccg acactttgga tgcagccacc
     5761 gaaaaactgc tgttgaacga caaatcgcct aaacgtaaag ccggcgaact cgacaaccgc
     5821 ggcagccatt tctacctcac cctctactgg gcgcaagaat tggcggcgca agacaaagat
     5881 gccgaactga aagccgcatt tacgccattg gcagccgctt tgaccgccga cgaagcgaaa
     5941 atcgttgccg agctttccgc cgtacaaggc aaagcggccg acatcggcgg ctactacgcc
     6001 gccaatcctg aaaaagccgc acaagtgatg cgtccgagcg taacctttaa tcaggcacta
     6061 aacgccttat aagcacaaag gtaaaaatgc cgtctgaaca ttcgtcgttc agacggcatt
     6121 agttatccct atctgcctga ttatgattaa acgccgcacc cattggatat
//
LOCUS       DAXHTR010000123         6088 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.123, whole genome shotgun sequence.
ACCESSION   DAXHTR010000123 DAXHTR010000000
VERSION     DAXHTR010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.123"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            57..1610
                     /locus_tag="ACFNML_001663"
     CDS             57..1610
                     /locus_tag="ACFNML_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HFC6393010.1"
                     /translation="MNRTYANFYEMLAAACRKNGNGTAVFDGKEKTAYRALKQEAEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAISAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFLFASVGLSKELAGLKAQTPIEKIIWTGKSRPVSETAEGDAFFEDVRRFPEKPDL
                     GRQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQTLLKRATVFLGIPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEAKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(1701..3728)
                     /locus_tag="ACFNML_001664"
     CDS             complement(1701..3728)
                     /locus_tag="ACFNML_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HFC6393011.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPNQVQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTVIWLNDGVAGGMLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            4017..6023
                     /locus_tag="ACFNML_001665"
     CDS             4017..6023
                     /locus_tag="ACFNML_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HFC6393012.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGAGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGIIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHMLHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLYRQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
BASE COUNT         1553 a         1856 c         1420 g         1259 t
ORIGIN      
        1 cggcatattg ttttcaaatg agggcgttcg ccgtccgcaa ccataaagga agtttcatga
       61 accggactta tgccaatttc tacgaaatgc tcgccgccgc ctgccgcaaa aacggcaacg
      121 gcacggcagt gttcgacggc aaggaaaaaa ccgcctaccg cgcgctcaag caggaggccg
      181 aagccgttgc ggcgtatctg caaaatatcg gcgtgaagtt cggcgacacg gtcgcgctgg
      241 cggtttccaa ttccacagaa tttattaccg cctatttcgc catctccgcc atcggcgcgg
      301 tcgccgtacc gatgaacaca tttttgaaaa acagcgaata cgcgtatatc ctgaacgact
      361 gcaaggcgcg cttcctgttc gcctcggtcg gcctgtcaaa agaattggcg ggcttgaagg
      421 cgcaaacgcc catcgaaaaa atcatttgga cgggcaaaag ccggccggtc agcgaaacgg
      481 cggaaggcga tgcctttttt gaagacgtgc gccgcttccc cgaaaaaccc gacttgggcc
      541 gacaaccccg gataaatgat ttggcacaca tcatctacac ctccggcacg acggggcatc
      601 ccaaaggcgc gctaatcagt tacgccaacc tgttcgccaa cctgaacggc atcgaacgca
      661 tctttaaaat ttccaaacgc gaccgcttta tcgttttcct gccgatgttc cacagcttca
      721 cgctgacggc tatggtgctg ctgccgattt atatggcgtg ttcgattatt ttggtcaaat
      781 ccgttttccc gttttccaat gttttgaaac agaccctgct caaacgcgcg accgtgtttt
      841 tgggcatacc cgcgatttac accgcgatga gcaaggcgaa aatcccttgg tatttcagat
      901 ggttcaaccg cattcgcctg tttatcagcg gcggcgcgcc tttggcggaa caaaccatcc
      961 tcgatttcaa agccaagttc ccccgcgcca aattgctgga aggctacgga ctgagcgaag
     1021 cctctcccgt cgtcgccgtc aatacgcccg agaggcaaaa agcccgcagc gtcggcatcc
     1081 ccctgcccgg tttggaagcc aaagccgtcg atgaagaatt ggtcgaagtg ccgcgcggcg
     1141 aagtgggcga actgattgtc aggggcggtt cggtgatgcg gggctacctc aatatgcctg
     1201 ccgccaccga tgaaaccatc gtcaacggct ggttgaaaac gggcgatttc gttaccatag
     1261 acgaagacgg cttcatcttt atcgtcgacc gcaaaaaaga tttgattatt tccaaaggtc
     1321 aaaatgtcta tccgcgcgag atcgaagagg aaatctacaa actcgatgcc gtcgaagccg
     1381 ccgccgtcat cggcgtgaaa gaccgttatg ccgacgagga aatcgtcgcc ttcgtccaat
     1441 tgaaggaagg tatggatttg ggcgagaacg aaatccgccg ccacctgcgt accgtgctgg
     1501 caaatttcaa aatccccaaa caaatccact ttaaagacgg gctgccgcgc aacgctacgg
     1561 gcaaggtatt gaaacgggtg ttgaaggagc agtttgacgg aaacaaatga acgccgtgcc
     1621 gtccgaagcc ccgtccggca aaaaaaatgc ggtgaatctg cttcaccgca tttttttggc
     1681 aaacgccgga gcgaggatat ttactctgcc aattccacct gctcgtgcgc catcagttcc
     1741 tgaccgacat catccaacaa catcaaataa cgttcgtagt ttttcaaaat gtcggcaatc
     1801 agctcgtcct tgttcatata ctgcacatcg tacccgacgc gcccgtcgaa aaaataagcg
     1861 tagggtttgt aagttgtctg atgccggata tgcggcagct tgccgtcgtt aatcaactgg
     1921 tcggatacat cctgcccgac agacttaatc ccgtacataa aatcgcgcat cgtctctttc
     1981 cgaatgacga actcgattgc gggctcgtcc tgatgaaaca tcttatcgac ccggacgctc
     2041 aagccgtatt cttccgaaag ctcccgttgc aactcgtgca tagcgggcga tgcggtatgt
     2101 ttgaggaatt ttaaaatatc ctgctcctgc gtctggctca ttatctgcac cagccgttct
     2161 ttccacttgc cgcccgtcca aaatacactg gtagggttaa cccgggtctc aaaatatttc
     2221 ttatccgcac tcaatccttt ccacaggctg aaacacatta tcagcatcag cagggcaaac
     2281 ggcagggaaa caatcagggt catagactgc aggttgccga gtccgcccga gcgcatcagc
     2341 aaaacggcaa cggcagacat cagcacgccc cacataaccg cctgccaccg tggcgcgctc
     2401 aagcctttgt cccgagaggt aatattgttc aggacataaa tcccggaatc ggcagaagtt
     2461 acaaaaaaca gagaaatgac cagcaggctg acgatgctcg tcaattcggg cagggggagg
     2521 taattaaaga atttaaaaag cagcgtttcc ggagaggagg tcatcttttc gagcattccc
     2581 cccgcaaccc cgtcattcag ccaaatcacc gtattgccga agacggtaaa ccacaaaacg
     2641 ccgaacaggc cggggatgag caaaaccccg aagacaaact cgcggatggt gcgccccttt
     2701 gaaatgcgcg cgataaacaa acccacaaac ggcgcccaag aacaccacca cgcccaataa
     2761 agcaccgtcc aagattcaaa ccacggcttg tgttcccgtt cgtacgcata agttttaaaa
     2821 ctgaggcgca ccagatttcc gaggtagttc cctatgttgt cgccgaatgc cgacaacagg
     2881 taaacagtgg gacccgccgc caaaacaaaa aacagcagca aaaacgcaag gctcaggttc
     2941 aactcgctca acaccttcac gcccttcccc acgccggata ttgccgaaac gacggcgagg
     3001 gacatgacgg cggcgataat caaaacctgc acgctgaagc tgttttcggc aatccagccc
     3061 atttcctgca atccggcgcc cagttgcgaa gccccgaacc ccaatgtggt gatgatgccg
     3121 aaaaaagtag caagcaacgc cataatatca atggcatcgc cgaaccttcc ggaaattttt
     3181 tctttcaaca ggggataaaa acaagaacgc agggcaagcg gcagcttgta gcggaaaccg
     3241 aaataagcca aagccaatgc aatcgtaccg tacaccgacc aagcgtgaac gccccaatgg
     3301 aacaccgtgt gcagcaatgc ctgctgctgc ctgtgttccg gcgtgccggc cgtaatgtcc
     3361 gaaaaataat gcatcaacgg ctctgccacg ccgaaaaaca tcagacccac gcccatcccg
     3421 gccgcaaaca gcatcgccag ccacgacagg aagccgaatt ccggcacatc ttcatcccgt
     3481 ccgagcctga tgtttcccaa actgctgacc gagagtatca gcaggaaacc cagaaaaatg
     3541 gaaaacgtta aaacataaaa ccagctgaac tcggtaaaaa tgacttcttt tgcccgatcg
     3601 agccacatct gcacctgatt cggcacggtt aaaaccaata ccaccaaaac acacacaaaa
     3661 aacaaagtcg tcaaaataac catcggatta aatgacgttc ggcgttctat aaattcagac
     3721 agggacaaac cttctcactc ctttgttaaa aacagacaaa cccggtcatc gggcaaagcg
     3781 gtcaaaacct gccggtaaaa taccggcttc cggatgacga aatgcacaaa ccggccgaaa
     3841 ttgttataat cgaaaaaatt aaaaatcaat acggattatt ctatcagatt gatttatcga
     3901 tatttattat atcattaata tagtttgatt tcaaaccggc ggaaaatgcg ccgcccaccg
     3961 caggcgcgcc ctccccgcta tcggggcgtt taacgccgga ttgccgatgc ggtacaatgg
     4021 ctgatatgaa gaaaattacc cctcaaaacc tgcgccccct gctttcggaa agcttgggac
     4081 ataccgattt tgtcaacgtc ctcaacgcac tgattaaatt tttgcgccgt ggcggcaaaa
     4141 aatgtgcggg ggaacgtttc gacctgatta tcgacacatt caaacaagac agggaattac
     4201 tgtcccgctt cagccggtgt ttttacatct ggctcgcgca aatacacatt tatccggcac
     4261 tcatcaaact cggcatcttc tcgcgccaca gctttgcccg ggaaatgggc atacgcatct
     4321 acgaacgctt cagcccgtca tataaagatt ttgccaactt gggcgaagtc ttcctttatc
     4381 ttttccattc cgaaaacgac gacaaatggc tgcaaacgct caatatccgc caatggctgg
     4441 ttttatacga actcatccgg agccacgccg agccgtccaa attgcagacg gcgggcatcc
     4501 gccttgccga tgcgcgtttg cgcgccatcg aaatgctgtc tgtctggacg gcatccgaag
     4561 ccatcgaacc cgacctcatc cgcatcgccc cgcgcctgct ggaagccgat tcttccttcg
     4621 tcgccctcca acgcgaaacc gccaaactgg tcgaacacta ccgcaacggc accacgcctt
     4681 acgacaccgc ccacctcgaa gtgatgttcg accaatgttt cagccagatt gactatttgc
     4741 gccgcaaagg gacgggcgcc ggctccggtt cgtcggtcaa agtcgcccac ctgctcgaac
     4801 ggctccggca gaccgtagac cgtctaaagc tgctcaccga catccaaacc ggtgccggca
     4861 acagcaaccg cctgaccatc gcgctgatga actccctcat ctacgcggcg gtcgaacaat
     4921 acagcacccg ccacctgcgc cgcagcagca tccgtatgct cgcccgcagc attaccgaaa
     4981 acaaaagcca ccacggcgaa cactacatca cccgcaaccg caaagaatat ttcaaaatgt
     5041 tctactcggc ggcaggcggc ggcatcatca tcgccctgat ggcgctgctc aaaatccgca
     5101 tcggcacact cggcctcagc cccttcctga cttccttgtc ggctgggttc aactacggca
     5161 tcggctttat gatcatccat atgctgcact gcaccgtcgc caccaaacag cccgcgatga
     5221 ctgccgccag ctttgccgaa caggtcgatc tcaacgaagg cggcaaagcg gtggacaaca
     5281 aactcgccaa gctcctcatc gacgtatgcc gttcccaaag tgtcgccgtt ttcggcaacg
     5341 tttccatcgc catccttttg gcgtgcgcca tatcgttcgg ctatgcccat ctgtaccggc
     5401 agcccatact cgatgcccac accgccgcct accagttcaa atccatagac atcatcgctt
     5461 acccgacgct gtggtatgcc gccattgcag gtctgtggct gttctgctcc ggcatcatcg
     5521 caggtttttt cgacaaccgc gccgactacc tcaacctgcg ccaacgcctg cccttcaacc
     5581 ccttgctgcg taaaatcatg cgccccgggc cccgccgcgt cctcgccgcc tacatccaca
     5641 aacactacgg ctcgttggca ggcaacttca tcttcgggat gctcttgggt atgaccggct
     5701 atttcggaca cctcctcggg ctgccgctgg acatccgcca cgtcgccttt tcctccgcca
     5761 acctcggcta tgccgccgtc agcggcaacg tcggtttggg cacgttcgta ctcggcattt
     5821 tcagcgtcct cgccatcggc ctggtcaacc tctgcgtcag cttcagcctc gccctcttcg
     5881 tcgccctgcg ctcgcgcggc acgaaaatcg gcagcatccg caatctgatt aagagttttt
     5941 ggaatcagat taaaagcaat ccctgcatac ttttcctccc gcccgccaaa gaacagggac
     6001 atcctccttc ggacaagcct tgaccggcaa tgccgtctga agcgggattc gccccgaata
     6061 ccgccctgat gcgggaaatc cccataaa
//
LOCUS       DAXHTR010000124         6022 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.124, whole genome shotgun sequence.
ACCESSION   DAXHTR010000124 DAXHTR010000000
VERSION     DAXHTR010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6022
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.124"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFNML_001666"
     CDS             complement(57..1460)
                     /gene="mtrE"
                     /locus_tag="ACFNML_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HFC6393013.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAALNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPEDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGTVGTGSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDVAKLRQQAQIVAYESAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYAAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(1514..4717)
                     /gene="mtrD"
                     /locus_tag="ACFNML_001667"
     CDS             complement(1514..4717)
                     /gene="mtrD"
                     /locus_tag="ACFNML_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HFC6393014.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LHATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADIGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSFEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(4729..5967)
                     /gene="mtrC"
                     /locus_tag="ACFNML_001668"
     CDS             complement(4729..5967)
                     /gene="mtrC"
                     /locus_tag="ACFNML_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HFC6393015.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEANLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGINLNRSRITAPISGFIGQSKVSEGTLLNAGDTTVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPTVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIVGITGAKKVTPKEWAPSENQA
                     ASPQSGVQTASEAKPASEAK"
BASE COUNT         1352 a         1706 c         1620 g         1344 t
ORIGIN      
        1 aaagaaatgc cgtctgaaac cttatcgttt cagacggcat tttattgccc gaccggttat
       61 ttgtcggttt gggtatcccg tttcaatccg ccgccgagtg ccttgtacaa atcggcaagg
      121 ttttcggcgc gggtcagttg tgccgacaaa gccgcaccct ccgccgcata gctgctgcgt
      181 tccgcatcga gcaaatcgag cgcgccggat acgccgtgct tgtaacgcag gccgaccaag
      241 cgcaacgctt ctttagaggc gcggctttgt ttgcttaaag cgtcataggc tttatccagc
      301 tgctcgcgcg ccgccaatgc gtttgccacg tcttgaaatg cggattggac ggcggattca
      361 taggcaacga tttgtgcctg ttggcgcagc ttggctacat caaggttcgc cttgttcgta
      421 ccccaggtaa aaatcggcag ggtaatagac ggcgcgaacg accaaacgcc cgtgccgctt
      481 ttgaacaacc cacccaattc ggcagaaccc gtaccgacgg ttccggtcag gcggatggat
      541 gggaaaaagg cggcgcgtgc cgcaccgata ttggcgtttg cctgtttgag cgcgtgttcg
      601 gcagcacgga tatcgggacg gtcgagcaat acttcggaac tcaaaccggc cggcagtttt
      661 tcaacaaaaa actgcttgtc cagcggcaaa ccggcaggca ggtcttcggg tatcggttgg
      721 ttaatcaagg ttgccaaggc attgcgcgcc tgttcgcggc tgcgcgcggc atgggcataa
      781 tcggctttgg cagattcgat cagggcttcc tgctgacgta gggcgacggc ggaaatcacg
      841 cctgccttgt aacgtaattc ggacagcttg taggtttcct cgcgcgtttt caaaacacgt
      901 tgcgccaaag acatcgcttc ttcggcgtaa cgttcgttga aataggcttt ggcaacggtg
      961 gcaatcaggc tcaaatgtgc cgcatcgcgg ttggcggtgc tggcgaaata gccttgcagt
     1021 gccgcctcgc tgctgctgcg tacacgcccg aacagatcga gttcgtaaga tgccgcaccc
     1081 agtccgactt tgtagctgct gcttacattg ccgccgctca agctgccttg gcgcgagtcg
     1141 ttcgcattgg cggcaagcgt gggcaggagg ttgttgcgct caatcatgta ttgtttgcgg
     1201 tagatttcgc tgttcaatgc ggcggtacgc aaactggtat tgcgctcgag tgcgatgtcg
     1261 atcagctttt gcaggcgcgg gtcggcaaaa tagtcatgcc aacctaaatc gaccgcacgg
     1321 atgccgctgt cggcggtatc gtttttgaac gtttcggcaa cttcgacttt gggctgctcg
     1381 tattggggaa tcatggtgca ggcggataat gcgaaggctg ctgcaacaga agtcaaggtg
     1441 gttttcaatg tagtatccat aaaaagtcct gatgccgtct gaaaacccgt gggcgttcag
     1501 acggcatggt tgcttaatgt tggctgtcgt ccgaaccggt gatgcccgct tcggcggcgt
     1561 gttttactgc catttcgtgt tcgtgcgcgg tttctttgaa gaatttgcgc accaccacat
     1621 agaaaagcgg aacaaggaac acggacaaga gcgtgccgat gagcatcccc cagaatacgg
     1681 tcgtgccgat ggcgcgctgg ctggcagaac ttgcaccacc ggcaatatac aggggaacca
     1741 cgcccaaaat aaaggcgaac gaggtcatga taatcggacg gaaacgcagg cgggcggctt
     1801 ccaaagcggc ttcgaccgcg cttttccctt gcgcttgaag gtctttggca aactcgataa
     1861 tcaaaatcgc gtttttcgca ctcaaaccca tcacggtaac gaaaccgact tgaaagtaga
     1921 tgtcgttggc gaacgaggga acgctgccca acagtccttc aaacaggttg cgcccggtta
     1981 cgcccgcagc cgcaccgatc aaacccaacg gaatcacaag gatgaccgcc agcggaatcg
     2041 accagctttc ataaagcgcg gcaagtacca aaaatacggc tgcaaccgcc aaaccgtaca
     2101 aaatcagggt ttgcgagccg ccttttgcct cttcgcgcga ctgtccgccc cattcgaagc
     2161 tgtaaccgcc gcccaattcg tcaaccattt tttgaaccgc cgccatagcc tgcccggtgg
     2221 aaacgccggt tgcaggcgaa gcggacagct tcatcgaagg ataaccgttg aagcgtacgc
     2281 tctgttccgt accgttttcc caagaaacag tagcaatggt ggaaagcggt acggcgacgc
     2341 cggatttgtt cggcacggtc aggttcaaaa tatcggcagg ctgcatacgg gcatcctcgt
     2401 cggcctgcac catcacgcgt tgcagacggc cttggttcgg gaagtcgctg acataagacg
     2461 aactcagcgc acttgccaat gcggtgcgga tgtcggcaaa cgaaatgcct tgcgccgccg
     2521 cggcggcacg gttgatgtcg attttcaact gcggcgagtc ttccaaaccg ccagcacgga
     2581 cggtgctggg gtcaaacaaa ccgctggcac gcattttctg aatcaactcg ttgcgcttcg
     2641 ccagcaatgc ggtatggccg gtattattgc ggtcttgcag gttgatgctc aggcccgaac
     2701 cattgcccaa ctccagaatc ggaggcggga cgacggcgat gccaaaaccg tctttaagcg
     2761 tccccatcat catacccgtc agcttgccgg caatcgcaac ggcatcgctg ccgggcgcgg
     2821 tacgctcgtt ccaatctttc aatatggcaa aacccatcgc catattctga ccgctgcccg
     2881 aaaagctgaa gccggaaacg gtaatgatgt tttctatttc aggaatgctt ttcgccagtt
     2941 gggtaacttg cgccaaagtc gcattggtgc gctcttgggt cgctcctgca ggcagttgca
     3001 cgctgaccat gacgaagcct tggtcttcgg tcggcaggaa tgaagtcggc aggcgcataa
     3061 acaggaacac gcccacaacc gccaagccga tatagacaac catcatgcgg aaagtcttac
     3121 gcagcacttt ggcaacccgg ccttcgtaac cgtgcgtcca actgttgaat ttcttgttaa
     3181 accagccgaa gaaacctttt ttctcttcgt gatgcccttt cgggattgtc ttcaacattg
     3241 tggcacacaa agcaggggta agggtcagcg caaggaaggc ggagaatgcg attgatgacg
     3301 ccatcgtcag ggcaaactgt ttgtaaatat tgcccgtcgc cccgctgaac atcgccaacg
     3361 gtacgaacac ggaaatcaga acggcggtaa taccgatgac cgcgcccgaa atctgaccca
     3421 tcgctttttt ggtcgcttct ttgggcggca agccttcacc cgccataatg cgctcgacgt
     3481 tttcaaccac cacaatcgcg tcatcgacca cgatgccgat gaccaaaacc atcgcaaaca
     3541 tggtcagtac gttaatcgac atgcccatat aagagatgaa ggcgaaaccg cccaacagcg
     3601 aaatcggtac gacgatggtc ggaatcagcg tataacggat gttttgcagg aagagataca
     3661 ttacgacaaa caccagcacc atcgcttcga ttaaagtgtg aatcactttt tcaatcgaaa
     3721 tttcgacgaa tttggaagta tcgtaagggg ttttccagct cataccctga ggaaagtatt
     3781 tttccaacgt cgccatgcgt tctttaaccg cctttgccgt cgccatcgca ttgccgctgt
     3841 tggacagcat caccgccata ccggtggtat ttacaccgtt cagacgggtt gaggaagaat
     3901 agtcttccat acccagtccg acccttgcca catccttcag gtaaacatta gaaccgtcgg
     3961 tattggcgcg gaggatgacg ttgccgaatt cttctgccgt acccaactgc ccttgcgccg
     4021 ttacggtagc cgtaaccgtc tgtccgcgaa cggcgggaag cgaaccgata gaacccgctg
     4081 aaatctggac gttctgggcg gacagcgcgc tgccgatatc ggcaaacgac aaattgtagt
     4141 tttgcagttt cttaggatca acccaaatcc gcatcgcgcg ttgcgcgccg aacaggcgta
     4201 cctgccccac gccttcgata cgctgcaact cgggaacgat attacgctgc gcgtagtcgt
     4261 tcatctcttc ggttgactgc acatccgacg aaagcatcac aatcatcagg aaattggaac
     4321 gcgccttgga tacggttacg ccgtattgct ggacggttgc tggcagcgtg ctcaatactt
     4381 cggaaagctt gttctgcact tccacctgcg ccaggttctc gtcggtatcg ggcgtaaagg
     4441 tcaggctcac gctgccgctg ccgctcgaat cggcagaagt ggacatataa tccaaacctt
     4501 ccacgccgtt catattccgc tcgatcacgg aaagcacgct gtcttccatt acctgcgcgg
     4561 acgcacccgg ataagtggcg tgcaaagtaa tggtcggggc ggcgacggac ggatattgcg
     4621 aaaccggcag gcttttgatg ccgaaaatac ccgccgcaat aatgaaaatc gaaataaccc
     4681 acgcaaaaat agggcggtcg ataaaaaatt tagccatcga tgccttcctt atttcgcttc
     4741 agaagcaggt ttggcttcag atgccgtctg aacgccggat tgaggcgagg cggcttggtt
     4801 ttcagacggc gcccattctt taggcgttac ctttttcgca cccgttatgc cgacgatact
     4861 gatgccttcc acaaccacct tgtccccgtc cttcagaccc gacgtaacaa tccaattcgt
     4921 accctgctgt tgcgcaaccg ttacctcgcg gggttccata ccgccttggg cattcacaat
     4981 catcacggta tctttcgcac cgcgcgttac cgcctgctgc ggcacaacaa atgcgttatc
     5041 caccgccact tggtccatca gcacgcgcac atacaggccg ggcatcagga tattctgatc
     5101 gttcggtacg gcggcgcgca gggtaatctg accggtcgat tcgttgacgg tcggatcggc
     5161 aaacagcagg cggccttttt cagggtaaac tgtgccgtcg tcaaatttga tgccgaccgc
     5221 aatcacacca tccgccgcca gcagtttgcc ttcggctatc tgacggcgca atttcatcac
     5281 ttcggatgca gactgggtaa cgttcacata catcggattg gtttggcgga tggttgccag
     5341 cacggtcgta tcgcccgcat tcaacagcgt accttcggaa actttggact gaccgataaa
     5401 gccggaaatc ggcgcggtaa tgcgcgaacg gttcagattg atgccggcag atttgatcgc
     5461 cgcctgtgct gctttgacac ctgcctcggc agaacgtttc gccgttaccg cagcatcgta
     5521 ttcctgccgg ctgacggctt cggcggcaac caaaggcttg tatcgcgcca aatccgcatc
     5581 cgctttggca agcgttgcct gagccgttgc cagttgcgcg cgcgcgcttt ccagatttgc
     5641 ttcataagtg gaactgtcga tctgatacag cggctgtccg gcacggacat aactgccttc
     5701 ttggaacagg cgtttttgga taatgccgcc aacttgcgca cggacatcgg cggtacgcag
     5761 cgattccaaa cgtcccggca actcgacggt caacgcaacg gtttgcggat ggacggttac
     5821 gacaccgacg acgggagccg gtgcttcccg acccgcaggc tgcccgccct gcgccgcgtc
     5881 tccgccttta ccgcaagacg acagtaccaa tgcaacggcg gcagccaacg cggccgcacg
     5941 catcgcctta aaagcataaa aagccattat ttatcctatc tgtctggttt gatgtaaagg
     6001 gttttgccaa tcaacaggca tt
//
LOCUS       DAXHTR010000125         5992 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.125, whole genome shotgun sequence.
ACCESSION   DAXHTR010000125 DAXHTR010000000
VERSION     DAXHTR010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.125"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..413)
                     /locus_tag="ACFNML_001669"
     CDS             complement(<1..413)
                     /locus_tag="ACFNML_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HFC6393016.1"
                     /translation="MLCPEKMSGMAGQYPYGVRSGLRRNGLKLWDIHFRMTRFIVARC
                     GLLFATLKGKTMKKMFVLFCMLFSCAFSLAAVNINAASQQELEALPGIGPAKAKAIAE
                     YRAQNGAFKSVDDLTKVKGIGPAVLAKLKDQASVGA"
     gene            complement(449..562)
                     /gene="rrf"
                     /locus_tag="ACFNML_001670"
     rRNA            complement(449..562)
                     /gene="rrf"
                     /locus_tag="ACFNML_001670"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(659..3550)
                     /locus_tag="ACFNML_001671"
     rRNA            complement(659..3550)
                     /locus_tag="ACFNML_001671"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3956..4031)
                     /locus_tag="ACFNML_001672"
     tRNA            complement(3956..4031)
                     /locus_tag="ACFNML_001672"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3996..3998),aa:Ala,seq:tgc)
     gene            complement(4037..4113)
                     /locus_tag="ACFNML_001673"
     tRNA            complement(4037..4113)
                     /locus_tag="ACFNML_001673"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4077..4079),aa:Ile,seq:gat)
     gene            complement(4215..5755)
                     /locus_tag="ACFNML_001674"
     rRNA            complement(4215..5755)
                     /locus_tag="ACFNML_001674"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1304 a         1758 c         1283 g         1647 t
ORIGIN      
        1 gcgccgaccg aagcctgatc cttcagcttc gccagcaccg cagggccgat gccctttacc
       61 ttggtcaaat cgtccacaga cttgaacgca ccgttttgcg cacggtattc cgcaatggcc
      121 ttcgccttcg cagggcctat gcccggcagc gcctccaact cctgctgcga agccgcattg
      181 atgtttaccg ccgcaaggga gaaggcgcag gagaacagca tacagaacag cacgaacatt
      241 ttcttcatgg tttttccttt aagggttgca aacaataaac cgcatcttgc gacgataaaa
      301 cgagtcattc taaaatgaat atcccaaagt ttcaagccgt tcctccgcaa acccgaccgg
      361 acgccgtacg gatactgtcc cgccatcccc gacatttttt ccgggcaaag caaaaacccc
      421 cgaataatcg ggggttttct gaaagggtgt ttggcagtga cctactttcg catggaagaa
      481 ccacactatc atcggcgctg agtcgtttca cggtcctgtt cgggatggga aggcgtggga
      541 ccaactcgct atggccgcca aacttaaact gttacaaatc ggtaaagcct taatcaatat
      601 attcggtaat gactgaatca gtcagtaagc ttttatctct tgaagttctt caaatgatag
      661 agtcaagcct cacgagcaat tagtatgggt tagcttcacg cgttaccgcg cttccacacc
      721 ccacctatca acgtcctggt ctcgaacgac tctttagtgc ggttaaaccg caagggaagt
      781 ctcatcttca ggcgagtttc gcgcttagat gctttcagcg cttatctctt ccgaacttag
      841 ctacccggct atgcaactgg cgttacaacc ggtacaccag aggttcgtcc actccggtcc
      901 tctcgtacta ggagcagccc ccgtcaaact tccaacgccc actgcagata gggaccaaac
      961 tgtctcacga cgttttaaac ccagctcacg taccacttta aatggcgaac agccataccc
     1021 ttgggaccga ctacagcccc aggatgtgat gagccgacat cgaggtgcca aactccgccg
     1081 tcgatatgaa ctcttgggcg gaatcagcct gttatccccg gagtaccttt tatccgttga
     1141 gcgatggccc ttccatacag aaccaccgga tcactatgtc ctgctttcgc acctgctcga
     1201 cttgtcggtc tcgcagttaa gctacctttt gccattgcac tatcagtccg atttccgacc
     1261 ggacctaggt aaccttcgaa ctcctccgtt acgctttggg aggagaccgc cccagtcaaa
     1321 ctgcctacca tgcacggtcc ccgacccgga tgacgggtct gggttagaac ctcaaagaca
     1381 ccagggtggt atttcaagga cggctccaca gagactggcg tctctgcttc aaagcctccc
     1441 acctatccta cacaagtgac ttcaaagtcc aatgcaaagc tacagtaaag gttcacgggg
     1501 tctttccgtc tagcagcggg tagattgcat cttcacaacc acttcaactt cgctgagtct
     1561 caggaggaga cagtgtggcc atcgttacgc cattcgtgcg ggtcggaact tacccgacaa
     1621 ggaatttcgc taccttagga ccgttatagt tacggccgcc gtttactggg gcttcgatcc
     1681 gatgctctca catcttcaat taaccttcca gcaccgggca ggcgtcacac cctatacgtc
     1741 cactttcgtg ttagcagagt gctgtgtttt taataaacag tcgcagccac ctattctctg
     1801 cgaccctcca gggcttacgg agcaagtcct taaccttaga gggcatacct tctcccgaag
     1861 ttacggtatc aatttgccga gttccttctc ctgagttctc tcaagcgcct tagaattctc
     1921 atcctgccca cctgtgtcgg tttgcggtac ggttcaattc aaactgaagc ttagtggctt
     1981 ttcctggaag cgtggtatcg gttacttcat gtccgtagac actcgtcatc acttctcggt
     2041 gttaagaaga cccggatttg cctaagtctt ccacctaccg gcttaaacaa gctattccaa
     2101 cagcttgcca acctaacctt ctccgtcccc acatcgcatt tgaatcaagt acaggaatat
     2161 taacctgttt cccatcgact acgcatttct gcctcgcctt aggggccgac tcaccctacg
     2221 ccgatgaacg ttgcgtagga aaccttgggc tttcggcgag cgggcttttc acccgcttta
     2281 tcgctactca tgtcaacatt cgcacttctg atacctccag cacactttac aatgcacctt
     2341 catcggccta cagaacgctc ccctaccatg ccagtaaact ggcatccgca gcttcggtta
     2401 tagatttgag ccccgttaca tcttccgcgc aggacgactc gaccagtgag ctattacgct
     2461 ttctttaaat gatggctgct tctaagccaa catcctggct gtctgggcct tcccacttcg
     2521 tttaccactt aatctatcat tggggacctt agctggcggt ctgggttgtt tccctcttga
     2581 caacggacgt tagcacccgc tgtctgtctc ccgaggaacc acttgatggt attctgagtt
     2641 tgccatgggt tggtaagttg caataacccc ctagccataa cagtgcttta cccccatcag
     2701 tgtcttgctc gaggcactac ctaaatagtt ttcggggaga accagctatc tccgagtttg
     2761 tttagccttt cacccctatc cacagctcat ccccgcattt tgcaacatgc gtgggttcgg
     2821 tcctccagta cctgttacgg caccttcaac ctggccatgg atagatcact cggtttcggg
     2881 tctacaccca gcaactcatc gccctattaa gactcggttt ccctacgcct cccctattcg
     2941 gttaagctcg ctactgaatg taagtcgttg acccattata caaaaggtac gcagtcacac
     3001 ctctagggcg ctcccactgt ttgtatgcat caggtttcag gttctgtttc actcccctcc
     3061 cggggttctt ttcgcctttc cctcacggta ctggttcact atcggtcgat gatgagtatt
     3121 tagccttgga ggatggtccc cccatattca gacaggattt cacgtgtccc gccctacttt
     3181 tcgtacgctt agtaccgctg ttgagatttc gaatacggga ctgtcaccca ctatggtcaa
     3241 gcttcccagc ttgttcttct atctcgacag ttattacgta caggctcctc cgcgttcgct
     3301 cgccactact tgcggaatct cggttgattt cttttcctcc gggtacttag atggttcagt
     3361 tctccgggtt cgcttctcta agtctatgta ttcaacttag gatactgcac agaatgcagt
     3421 gggtttcccc attcggacat cgcgggatca ttgctttatt gccagctccc ccgcgctttt
     3481 cgcaggctta cacgtccttc gtcgcctatc atcgccaagg catccacctg atgcacttat
     3541 tcacttgact ctatcatttc aagaacttct ttgactttgc ctaacattcc gttgactaga
     3601 acatcagact tgaatttcct actttgataa agcttactgc tttgttgtgt cttaatcctg
     3661 ccttttgtgt ttcaggatta agtcgataca atcatcaccc aaatactgtg tttgttttct
     3721 tttctctcgc gagagatttt tatcctttgc aaagaataaa aaatcaaaac aaacgctttg
     3781 tctttgtttg ttgatttcgg ctttccaatt tgttaaagat cgatgcgttc gatattgcta
     3841 tctactgtgc aaatcaaaac gagctgatta ttatatcagc attttgttct tggtcaagtg
     3901 tgacgtcgcc ctgaatggat tctgttccat tcttccgttt tgatttgtac agtattggtg
     3961 gaggcaaacg ggatcgaacc gatgaccccc tgcttgcaaa gcaggtgctc taccaactga
     4021 gctatgcccc cgttcttggt gggtctggga ggacttgaac ctccgacccc acgcttatca
     4081 agcgtgtgct ctaaccagct gagctacaaa cccggattct cttcttaagc gaatcttgcc
     4141 ttcactcaag cttcttccgc atctttttca gtttaccgat aagtgtgaat gcctaaagcc
     4201 tcttctttct ctagaaagga ggtgatccag ccgcaggttc ccctacggct accttgttac
     4261 gacttcaccc cagtcatgaa gcataccgtg gtaagcggac cccttgcggt taccctacct
     4321 acttctggta tcccccactc ccatggtgtg acgggcggtg tgtacaagac ccgggaacgt
     4381 attcaccgca gtatgctgac ctgcgattac tagcgattcc gacttcatgc actcgagttg
     4441 cagagtgcaa tccggactac gatcggtttt gtgagattgg ctccgcctcg cggcttggct
     4501 accctctgta ccgaccattg tatgacgtgt gaagccctgg tcataagggc catgaggact
     4561 tgacgtcatc cccaccttcc tccggcttgt caccggcagt ctcattagag tgcccaactg
     4621 aatgatggca actaatgaca agggttgcgc tcgttgcggg acttaaccca acatctcacg
     4681 acacgagctg acgacagcca tgcagcacct gtgttacggc tcccgaaggc actcctccgt
     4741 ctctggagga ttccgtacat gtcaagacca ggtaaggttc ttcgcgttgc atcgaattaa
     4801 tccacatcat ccaccgcttg tgcgggtccc cgtcaattcc tttgagtttt aatcttgcga
     4861 ccgtactccc caggcggtca atttcacgcg ttagctacgc tactaagcaa tcaagttgcc
     4921 caacagctaa ttgacatcgt ttagggcgtg gactaccagg gtatctaatc ctgtttgcta
     4981 cccacgcttt cgggcatgaa cgtcagtgtt gtcccaggag gctgccttcg ccatcggtat
     5041 tcctccacat ctctacgcat ttcactgcta cacgtggaat tctacctccc tctgacacac
     5101 tcgagtcacc cagttcagaa cgcagttccc gggttgagcc cggggatttc acatcctgct
     5161 taagtaaccg tctgcgcccg ctttacgccc agtaattccg attaacgctc gcaccctacg
     5221 tattaccgcg gctgctggca cgtagttagc cggtgcttat tcttcaggta ccgtcatcag
     5281 ccgctggtat tagcaacagc cttttcttcc ctgacaaaag tcctttacaa cccgaaggcc
     5341 ttcttcagac acgcggcatg gctggatcag gcttgcgccc attgtccaaa attccccact
     5401 gctgcctccc gtaggagtct gggccgtgtc tcagtcccag tgtggcggat catcctctca
     5461 gacccgctac tgatcgtcgc cttggtaggc ctttacccca ccaactagct aatcagatat
     5521 cggccgctcg aatagcgcaa ggcccaaagg tcccctgctt tctctctcaa gacgtatgcg
     5581 gtattagctg atctttcgat cagttatccc ccactactcg gtacgttccg atatgttact
     5641 cacccgttcg ccactcgcca cccgagaagc aagcttctct gtgctgccgt ccgacttgca
     5701 tgtgtaaagc atgccgccag cgttcaatct gagccaggat caaactctta tgttcaatct
     5761 ctaacttttt aacttctggt ctgcttcaaa gaaaccaaca ggacaatgtt caaaacatta
     5821 tcttgtctgt ctttcaaaca gtgtgaggct caaggcactc acacttatcg gtaatctgtt
     5881 ttgttaaaga gcgttgcgaa ttataaagta tctcttccgc ctgtcaagat atctctcgat
     5941 atccccaaca ttctgtgcta tacttttcag ttcgtccgcc gcttctgcag ca
//
LOCUS       DAXHTR010000126         5992 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.126, whole genome shotgun sequence.
ACCESSION   DAXHTR010000126 DAXHTR010000000
VERSION     DAXHTR010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.126"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(141..728)
                     /locus_tag="ACFNML_001675"
     CDS             complement(141..728)
                     /locus_tag="ACFNML_001675"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HFC6393017.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFNACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRFAALS"
     gene            891..2297
                     /gene="dnaB"
                     /locus_tag="ACFNML_001676"
     CDS             891..2297
                     /gene="dnaB"
                     /locus_tag="ACFNML_001676"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFC6393018.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
     gene            2599..3267
                     /locus_tag="ACFNML_001677"
     CDS             2599..3267
                     /locus_tag="ACFNML_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HFC6393019.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKGGAPNNKCDFGKKGQGMLAFGDKNGNK
                     TYDGDTADVLLRSVVLNDDINDKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYST
                     NQDLTNTSRFVYSDGYIQIVLTDARAVSDADKKFRSAVVLINSSGRVEVCPRNDRRTV
                     CQYK"
     gene            3297..3917
                     /gene="pilV"
                     /locus_tag="ACFNML_001678"
     CDS             3297..3917
                     /gene="pilV"
                     /locus_tag="ACFNML_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HFC6393020.1"
                     /translation="MKNNDCFRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMGNNTLSHTLSAVDGEFA
                     IDAIKTKTQLAEAQLKRFSYELKNALPDAAAIHYAVCKDSSGNAPTFSGNAFSSNCDN
                     KANGDTLIKVLWVNDSAGDSDISRTNLEVSGDNIVYTYQARVGGRE"
     gene            3914..4867
                     /locus_tag="ACFNML_001679"
     CDS             3914..4867
                     /locus_tag="ACFNML_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HFC6393021.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAIQQDLRNAATLIVRDARMAGSFGCFNMSEHIEQDVVSDVTQKDSL
                     FSLKRNSTRNSTNKLIPIAESSNIGYQGFIQRLNALIFQYGIDDVNASADTTVVSSCA
                     AISKPGKQILNLEDVKKELKIVSQDKERNGNIARQRHVVNAYAVGRIAGEEGLFRFQL
                     NEKGEWGNPQLLVKKINKMDIRYIYVSGCPEDDDAGKEETFRYTDKFNSAQDAVTPAG
                     VEVLLSSGTDTKIAASSDNHIYAYRIDATIRGGNVCANRTL"
     gene            4846..5439
                     /locus_tag="ACFNML_001680"
     CDS             4846..5439
                     /locus_tag="ACFNML_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HFC6393022.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGKPTVEAVKRSCPANSTNLCIDNKGTKYKKGTQSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            5444..5917
                     /locus_tag="ACFNML_001681"
     CDS             5444..5917
                     /locus_tag="ACFNML_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX family type IV pilin"
                     /protein_id="HFC6393023.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSGYKMNPKIAEKYNVSVDFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDAASAQAHLETLSSDAGCEAFSNRKK"
BASE COUNT         1652 a         1312 c         1536 g         1492 t
ORIGIN      
        1 tgacagtaac cgtagcaact gaaccgtcat tcccacggaa gtgggaatct agaaataaaa
       61 agcagcaggc atttatcgga aataactgaa attcaatacc gcaaaaatct acccgaaatg
      121 atatagcgga ttaacaaaaa tcaggacaag gcggcaaaac gtttggcgac ttcgtcccag
      181 ttgacgattt cccagaaacc tttcaggtag ttaggacggc tgttgcggta gtcgatgtag
      241 taggcgtgtt cccacacgtc gcaggtcagc agcggcgtgt tttcagtggt cagcggcgta
      301 gcggcgttgg aagtggaaac caaatccaat ccgccggcag gggtttttac cagccacgcc
      361 caaccggagc cgaaagtacc ggccgcgcag gcattgaacg cttcttggaa tttctcgaag
      421 ctgccccatt tcgcgtcgat ggcggcggcc agttcgccgg aaggtttgcc ttgacctttg
      481 ggcgtgaaac ccagccagta gaaagtgtgg ttccaagttt gggctgcgtt gttgaacacg
      541 ccgcccgaag attttttcac aatctcttcc aaaggcaggt tttcaaattc ggtgcctttg
      601 atttgattgt tcaggttggt gatgtaggtt tgatggtgtt tgccgtagtg gaattccaaa
      661 gtttctttgc tcagatgcgg ggacaatgcg tccagttcat aaggcagttg cggcagctta
      721 tgttccattt tgtgctccta atattgtttt aaatgttgta ttttggcagt gttgctgcaa
      781 atagctcagc agcgcacata ttttacctgt tttgagggat ggaaaccaat taaacctgct
      841 ttacgctata atagaaggtt gcaatttcgg cacgacagat aggatgtacc atgaacgatt
      901 acacagctat gccgtctgaa gaccgtgagg tcggcgcatt gtcgctgcct ccacattcga
      961 tggaggcgga acaatccgtt ttgggcggtt tgatgctgga aaatccggct tgggacagga
     1021 ttgccgatgt ggtttccggt gaagacttct accgccacga acaccgcctg attttccgat
     1081 ccattgctaa attaatcaat gagagccgtc ccgccgatgt gattacggtt caggaagatt
     1141 tgcagcggaa cgaagaattg gaagcggcag gcggattcga atatctgatt acgctggcgc
     1201 aaaacacccc gtctgccgcc aacatccgcc gctacgccga aatcgtgcgc gagcgttcca
     1261 tcatgcgcca actcgccgaa gtggggacgg aaatcgcccg cagcgcatac aatccgcaag
     1321 gcagggacgc ggggcagctt ttggacgagg cggaaaacaa agtattccaa atcgccgaaa
     1381 gcaccgccaa atccaagcag ggctttttgg agatgcccga tttgctgaaa gaagtcgtac
     1441 agcgcatcga tatgctctac tcgcgcgaca atcccgatga agttaccggc gtgccgacag
     1501 ggttcatcga cctcgacaaa aaaacctcgg gtctgcaacc cggcgacctg attatcgttg
     1561 ccggtcgtcc gtctatgggt aagaccgcct tttctatcaa tatcgccgaa cacgttgccg
     1621 tagaaggcaa gctgccggtt gccgtcttct caatggaaat gggcggggcg caactggtca
     1681 tgcgtatgct cggctcggtc ggacggttgg atcaaagcgt tttgaaaacc ggcaggctcg
     1741 aagacgaaca ctggggtcgc ctgaacgaag cagtcgtcaa actctccgac gcgcccgtgt
     1801 acatcgacga gaccccgggt ctgaccgcgc tcgaactgcg cgcccgtgcc cgccgtctcg
     1861 cccgtcaatt taacaataag cttggattga ttgtcatcga ctacctgcaa ctgatggcag
     1921 gatccggccg ttccgacaac cgagcttcgg agctgggaga gatttcacgt tcgctcaaag
     1981 cgttggcgaa agaattgcaa gtccccatca tcgccctgtc gcaattgagc cgcacggtcg
     2041 aatcgcgtac cgacaaacgc ccgatgatgt ccgaccttcg cgaatccggt gctatcgagc
     2101 aggatgccga cctgattatg tttatgtacc gcgacgaata ctacaaccaa gactcgccca
     2161 tgaaaggcct tgccgaatgt atcatcggca aacaccgcaa cggtcccgtc ggtaaaatct
     2221 tcctcacatg gacgggacaa ttcaccaaat tcgacaatgc tgcctatatt cccgaggagg
     2281 caaagataga ggattaaatg gctatataaa aatttattag gcgaaatcag gcaaaatcgt
     2341 ttaaaatcat gcttagagat tgccctaaaa aataaaacgc ggtcttgagg catttttgca
     2401 ttcagcccgc atataattga aaatatagtg gattaacaaa aatcaggaca aggcaacgaa
     2461 gccgcagacc gtacaaatag tacggaaccg attcacttgg tgcttgagca ccttagagaa
     2521 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttgaatttaa tccacgatac
     2581 attaccagtt aacgtcctat gtgtacacga aaacaacaag gtttcacgct aacagagctg
     2641 ctcatcgtga tggtcattgc agccattatg gcgatgatag ccctccccaa tatgagccaa
     2701 tggattgcat cccgccgcat tgccagtcat gcggagcgga ttgccaacct tttgcgtttc
     2761 tccaggggag aagccgtccg gctcaatctt cctgtctata tctgtcctgt tcaagttaaa
     2821 aaaggcggtg cgcccaacaa taaatgtgac ttcggcaaga aggggcaggg aatgttggct
     2881 tttggcgata agaatggcaa taagacatat gacggcgata cggcggatgt tctccttcgc
     2941 agtgtggtat tgaatgatga tatcaatgat aagcggatta attatgcctt taaccatatc
     3001 gctttcggtc aaacgcagcc gaccgctgac cgtgtagttt ggacattcaa tcaaaacggg
     3061 acgttcggtt attctaccaa tcaggatctc acgaatactt ccagatttgt ttactccgac
     3121 ggttatatcc aaatcgtgct gacagatgcg agagcagttt cagatgccga taagaaattc
     3181 cgttcggcgg tggttttgat taacagcagc ggcagggttg aggtttgtcc tagaaacgat
     3241 aggcgtaccg tatgccaata taaatgattg ttttagtcgg tttgggaaat aaaaaaatga
     3301 agaataatga ttgcttccgc ctgaaaaacc cccagtccgg tatggcgctg atagaagtct
     3361 tggttgctat gctcgttctg accatcggta ttttggcgtt actgtctgtg cagttgcgga
     3421 cagtcgcttc cgtcagggag gcggagacac aaaccatcgt cagccaaatc acgcaaaacc
     3481 tgatggaagg aatgttgatg aatccgacca ttgattcgga cagcaacaag aaaaactata
     3541 atctttacat gggaaacaat acactatcgc atacactatc agctgtggat ggtgagtttg
     3601 cgattgatgc cataaaaact aagacacagt tggcagaggc acaattgaag agatttagtt
     3661 atgagctgaa aaatgccttg ccggatgcgg cagccatcca ttacgccgtc tgcaaggatt
     3721 cgtcgggtaa cgcgccgaca ttctccggca atgctttttc ttcaaattgc gacaataagg
     3781 caaacgggga tactttaatt aaagtattgt gggtaaatga ttcggcaggg gattcggata
     3841 tttcccgtac gaatcttgag gtgagcggcg acaatatcgt atatacctat caggcaaggg
     3901 tcggaggtcg ggaatgagac gtaaaatgct aaacgtacca aaaggcagtt atgatggtat
     3961 gaaaggtttt accattattg aatttttggt tgcgggcatg ctcagtatga ttgtcctgat
     4021 ggcggtcgga tcgagttact tcacatcccg gaaattaaat gatgcggcaa acgagcgtct
     4081 tgctattcaa caggatttgc ggaatgcggc aacattaatt gtccgcgatg caagaatggc
     4141 ggggagcttc ggttgtttca atatgtctga gcatatcgaa caggatgttg tttccgatgt
     4201 gacgcaaaaa gactctcttt tttccttaaa aaggaacagt acaaggaaca gtacaaataa
     4261 acttatcccc atagcggaat cttcaaatat cggatatcag ggttttatcc agcgccttaa
     4321 cgcattgatt ttccaatacg gaatcgatga tgttaatgca agcgccgata ctaccgtcgt
     4381 cagcagctgt gccgcaatat cgaaaccggg taagcaaatc cttaatttag aagatgtaaa
     4441 aaaagaattg aagattgtga gtcaggataa ggagcgaaat ggcaatatag cgcgtcaaag
     4501 gcatgtggtc aatgcctatg cggtcggcag gattgccggt gaggaaggtt tgttccgctt
     4561 ccaattgaat gaaaagggcg agtggggtaa tcctcagttg ttggttaaaa agattaataa
     4621 aatggatata cggtatattt atgtttccgg ctgtcctgaa gatgacgatg ccggcaaaga
     4681 ggaaacattc agatatacgg ataaattcaa cagcgcccaa gatgctgtta cgcctgccgg
     4741 ggtggaggtt ttattgagta gcggtactga taccaagatt gccgcttctt cagacaatca
     4801 tatttatgct taccgtatcg atgcgacaat acgcggggga aatgtatgcg caaacagaac
     4861 actttgacgg gaatcccgac ttctgacgga cagagggggt ttgcactgtt tatcgtgctg
     4921 atggtgatga tcgtcgtggc ttttttggtt gtaactgccg cgcagtctta caataccgag
     4981 cagcggatca gtgccaacga atcagacagg aaattggctt tgtctttggc cgaggcggct
     5041 ttgcgggaag gcgaacttca ggttttggat ttggaatatg atacggacag taaggttaca
     5101 tttagcgaaa actgtggaaa aggtctgtgt accgcagtga atgtgcggac aaataatgat
     5161 aatgaagagg cttttgacaa tatcgtggtg caaggcaagc ccaccgttga ggcggtgaag
     5221 cgttcttgcc ctgcaaattc taccaacctg tgcattgaca ataaagggac gaaatataag
     5281 aaaggcacgc aaagcgtcag caaaatgcca cgttatatta tcgaatattt gggcgtgaag
     5341 aacggagaaa atgtttatcg ggttactgcc aaggcttggg gtaagaatgc caataccgtg
     5401 gtcgtccttc aatcttatgt aagcaataat gatgagtaat aaaatggaac aaaaagggtt
     5461 tacattgatt gagatgatga tagtcgtcgc gatactcggc attatcagcg tcattgccat
     5521 accttcttat caaagttata ttgaaaaagg ctatcagtcc cagctttata cggagatggt
     5581 cggtatcaac aatatttcca aacagtttat tttgaaaaat cccctggacg ataatcagac
     5641 catcaagagc aaactggaaa tatttgtctc aggctataag atgaatccga aaattgccga
     5701 aaaatataat gtttcggtgg attttgtcaa taaggaaaaa ccaagggcat acagcttggt
     5761 cggcgttcca aagacgggga cgggttatac tttgtcggta tggatgaaca gcgtgggcga
     5821 cggatacaaa tgccgtgatg ccgcttctgc ccaagcccat ttggagacct tgtcctcaga
     5881 tgccggctgt gaagccttct ctaatcgtaa aaaataaggt tgttttgcca ataccgtctg
     5941 aaaatcaatg ttcagacggt atttttatgg gtatagtgga ttaacaaaaa tc
//
LOCUS       DAXHTR010000127         5949 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.127, whole genome shotgun sequence.
ACCESSION   DAXHTR010000127 DAXHTR010000000
VERSION     DAXHTR010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5949
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.127"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..208
                     /locus_tag="ACFNML_001682"
     CDS             <1..208
                     /locus_tag="ACFNML_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393024.1"
                     /translation="FSELYMFQQRTAHQAPHCVLPERDYPPIRFYRYKQMGFNRKGMG
                     IKSISDTSRAMPSENQSPLSDGIV"
     gene            285..1232
                     /locus_tag="ACFNML_001683"
     CDS             285..1232
                     /locus_tag="ACFNML_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6393025.1"
                     /translation="MNILSKISSFIGKTFSLWAALFAAAAFFAPDTFKWAGPYIPWLL
                     GIIMFGMGLTLKPSDFDILFKHPKAVIIGVIAQFAIMPATAWLLSKLLNLPAEIAVGV
                     ILVGCCPGGTASNVMAYLARGNVALSVAVTSVSTLISPLLTPAIFLMLAGEMLEIQAA
                     GMLMSIVKMVLLPIVLGLIVHKVLGSKTEKLTDALPLVSVAAIVLIIGAVVGASKGKI
                     MESGLLIFAVVVLHNGIGYLLGFFAAKWTGLPYDAQKTLAIEVGMQNSGLAAALAAAH
                     FAAAPVVAVPGALFSVWHNISGSLLATYWAAKAGKHKKP"
     gene            complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="ACFNML_001684"
     CDS             complement(1385..2509)
                     /gene="rluD"
                     /locus_tag="ACFNML_001684"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFC6393026.1"
                     /translation="MQNTSFDNESDYSDDSDFASASGTENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQVPRAGIVHRLD
                     KETSGLMVVAKTLPAQNSLVQQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAADPVYG
                     NPRHPCGDTVKEAVKSLGARQALHAYRLSFTHPKTGETVSFEAPIPDDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            2508..3311
                     /locus_tag="ACFNML_001685"
     CDS             2508..3311
                     /locus_tag="ACFNML_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFC6393027.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEMSVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(3369..5588)
                     /locus_tag="ACFNML_001686"
     CDS             complement(3369..5588)
                     /locus_tag="ACFNML_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HFC6393028.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWRAEAVSGVPLTVEVADMPRSDGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRAWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPSAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVFPFAPLQQAGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRSPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQTGIVPSLNASGIRYLDYLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVAGGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         1462 a         1870 c         1424 g         1193 t
ORIGIN      
        1 tttctccgag ctttatatgt ttcaacagag gacggcacat caagcaccgc actgcgtgtt
       61 gcccgaacga gactaccctc cgattagatt ctatcgctat aaacagatgg gtttcaaccg
      121 aaaaggaatg gggataaagt ccatttccga cacctctcgg gcgatgccgt ctgaaaacca
      181 atctccactt tcagacggca ttgtttaggc acggtcattt tgcaaattaa ggttaaggcg
      241 tgtatgcttg ccttccctta aaaacaaaca cgcaacgcat tattatgaat atcctcagta
      301 aaatcagcag ctttatcgga aaaacatttt ccctctgggc cgcgctcttt gccgccgccg
      361 cttttttcgc gcccgacacc ttcaaatggg cggggcctta tattccttgg ctgttgggca
      421 ttattatgtt cggtatgggt ttgacgctca aaccttccga cttcgatatt ttgttcaaac
      481 atcccaaagc cgtcatcatc ggcgtaatcg cacaattcgc cattatgccg gcaaccgcct
      541 ggctgctgtc caaactgttg aacctgcctg ccgaaatcgc ggtcggcgtg attttggtcg
      601 gctgctgccc gggcggtacg gcttccaatg tgatggccta tctggcgcgc ggcaatgtgg
      661 ctttgtcggt tgccgttacg tctgtttcca ccctgatttc cccattgctg actcccgcca
      721 tcttcctgat gcttgccggt gaaatgctgg aaatccaagc ggccggtatg ttgatgtcca
      781 tcgtcaaaat ggttttgctc cccattgttt tgggtttgat tgtccataag gttttgggca
      841 gtaaaaccga aaagctgacc gatgcgctgc cgctggtttc cgttgccgcc atcgtgctga
      901 ttatcggcgc ggttgttggg gcaagcaaag gcaagattat ggaaagcggc ctgctgattt
      961 ttgcggttgt cgtactccac aacggcatcg gctacctgct cggcttcttt gccgccaaat
     1021 ggaccggcct gccttatgat gcacaaaaaa cgctggccat cgaagtcggt atgcaaaact
     1081 cgggcctggc cgccgcgctt gccgccgcac actttgccgc tgcgccggtc gttgccgttc
     1141 cgggcgcatt gttcagcgtg tggcacaata tctccggctc gctgctggca acttattggg
     1201 cggccaaagc cggtaaacat aaaaaaccct aaacgccttt catcataatg ccgtctgaac
     1261 ggggcaaacg aaaccggcgg tttccgcccg accccttcag acggcctttg tttgaaatcg
     1321 gcagggacga ttgtccggct gctccgattc ggctgcctgc gttcgccccg cctttcaagc
     1381 cgcctcactc ccttacataa accacttcga catcgtagtc gtcttcgttc caatcgtcat
     1441 cgtcgtccgc gccgaatttg tcctgccatt cctcttcatt gctcaaagac gaatccaaac
     1501 cggcttcaag acggaggaca gacaacaaat gatatatgtc gtccggaatc ggtgcttcaa
     1561 acgaaacggt ttcgccggtt ttcggatgag tgaaacttaa gcggtaggcg tgcaatgcct
     1621 gacgcgcacc caaactttta acggcttctt tcaccgtgtc gccgcacgga tggcgcgggt
     1681 tgccgtaaac cgggtcggcg gcaagcggat ggttggcctc acgcatatgg acgcggattt
     1741 ggtgcgtcct gcctgtttcg agcgagcatt cgatatagct gtgcgccaga tagcgttcca
     1801 acactttgac gtgggtaacg gctggtttgc cgccgaattt gacgactgcc attttcaggc
     1861 ggttgtgcgg atcgcgtccg atttgggttt cgattttacc gtcaaaggga acgatgccgt
     1921 tggcgacggc gcggtaaatg cgtttgaccg tgcgctcttg aagctgttgc acgagggaat
     1981 tttgcgccgg cagggttttg gcaaccacca tcagcccgct ggtttctttg tccaaacggt
     2041 gtacgatgcc tgcacgaggt acttggctca attcgggaca gtgcgccaag aggccgttga
     2101 gcagcgttcc cgtccagttg ccggccgccg gatgcaccac cagtccggcc ggtttgttga
     2161 cgacgatgac ggtatcgtct tcgtaaacaa tatccaaagc cataggctct ggaacaaacg
     2221 ccagattttc ctcactcgga cggacggtta cacaaatttg ctcgccgcct atcattttgt
     2281 ctttgggttg cgaaggttta tcgtttacaa taaccgcgcc ttctttaatc catgatgtca
     2341 ggcggctgcg cgagtagtcg ggcagaagtt tcgccaacac cgcatccaac cgcccgccgg
     2401 caagctcgag cggaacggtc agaccgatac ggttttcagt tccggaagct gacgcaaagt
     2461 ctgaatcgtc gctataatcg gattcattat caaaggaagt attctgcatg aaaaaaattc
     2521 ttttaacggt ttcattaggt ttggcactga gtgcctgtgc cactcaaggt acggtcgata
     2581 aagatgccca gattacccaa gattggagtg tggagaagct ctatgccgaa gcccaggacg
     2641 aattgaacag cagcaattat acgcgggctg tcaagttata cgaaatcttg gaatcgcgct
     2701 tccccaccag ccgccatgcc cggcagtcgc aactggatac cgcatacgcc tattataaag
     2761 acgatgaaaa agacaaagct ctggcggcaa tcgaacgctt ccgccgcctt cacccgcaac
     2821 acccgaatat ggattacgcg ctgtatctgc gcggcttggt gctgttcaac gaagaccagt
     2881 ccttcttgaa caaactggcc tcgcaagact ggtccgaccg cgacccgaaa gccaaccgcg
     2941 aagcgtacca ggcgtttgcg gaactcgtcc aacgcttccc caacagcaaa tacgccgccg
     3001 atgcgaccgc acgcatggtc aaactggtcg atgcactggg cggcaatgaa atgtcggtgg
     3061 cgcgctacta catgaaacgc ggtgcataca tcgccgccgc caaccgcgcc caaaaaatca
     3121 tcggcagcta ccagaatacg cgttatgtcg aagaatcgct cgccatattg gaacttgcct
     3181 accaaaaact cggcaaacca cagcttgccg ccgatacgcg ccgcgtgttg gaaaccaact
     3241 tcccgaaaag cccgtttttg acgcacgctt ggcagcccga cgatatgccc tggtggcgtt
     3301 actggcatta agcccatctt cgtaatgccg tctgaagccg tttcagacgg cattttatgt
     3361 ttcaaacctc attcaaacgg ttttttctgc caatagaatt tataccctct caaacgttga
     3421 gccttcacgc cgccgcgccc caagccgaat tgcagcgcgc ctgacaaatc ggtacgcagc
     3481 agtttgatgc cgtgtgcgcg gacacggttc tgcaccgctt cggtcggatg tttgtaggcg
     3541 ttggcataac cgcttgaagc aacagcatat tcgggcgaga cggcattgag gaacacgccc
     3601 gacgaggacg tattgctgcc gtgatgtccc aacaccaaaa cctggctgta caggttgcct
     3661 ccatacttgc cgaccaggct ttcctcaccc ttcgtatcca aatcgcccgt taccagcagt
     3721 gccgcaccgc ccgccacaac acgcaaaaca caacttttcc cattatcatc gatgtttttg
     3781 cgttcagacg gcatcaaaaa ctcgaaatcc acgccgtccc attgccaacg ctgttccgca
     3841 caatgccgcg ccccctcata aaattccggc tgtccggcat aaatcccgcc gttcggtatc
     3901 ttgccgactg cctgaaaacc gccgtcgtgg tcgctgtcgt gatgcgacaa aaccaaataa
     3961 tccaaatacc tgattcccga cgcattcagg ctcggcacaa tccccgtctg cgccgccgct
     4021 gccgttccgg tgtcaaacaa aagatgatga tttgccgtct gaaccgacac cgacaaaccc
     4081 tgccccgcat cccaaaccgt aaccgcaacc tcattttccg gcacgccggg tgaacggtaa
     4141 aacacaaacc ctgccaacag caacaccgcc cacggacgca aacccaagcc gcgcggcagc
     4201 agcaacagca aagcggcaca caccgccaac accaacagcg gcaaaggtgc ggaggcaacg
     4261 gcaaactcgg gcgacacatc ggcaagccac accaaaaacc gcaaagtgta ttccgccaaa
     4321 aacgcccccg cctgctgaag cggcgcaaac ggaaaaaccg aacccagcaa cgccagcggc
     4381 gtcaataccc aagaaaacca cggaattgcc accgcattga ccaaagggct gattaaaggc
     4441 agcgaagcaa acagataacc gagcaaaacc agcgacaaca ccgaagccgc ccactgcccg
     4501 cgcaaagcag tttgccgttt tccctcgtgc aaacgccccg agcacgccca tatcagggcc
     4561 gccaccaaac cgaaagacag ccaagtcccc acacccaaga ccgccgacgg gtcgaacagc
     4621 agcaccgccg ccaacgcctg ccaccacgtc gcccacgccg acaatcttcc cctgcgccaa
     4681 gcccacgcaa acgccgccaa catcaaaacg ctgcgctgcg tcggcacgga aaaaccggca
     4741 agcagcgcgt aaaacagcgc gcctgcacac cccgccgcca aaacccacgc gcgcggccgg
     4801 gcaggaaggc gcggggaaca ggcaagcagc cgcttcgcca gccacgcaaa cattaccgcc
     4861 accatcgtaa cgtgcaaacc cgaaatactg accaaatgcg tcagccccaa cggccggaac
     4921 gcctgccaca attcggggcg caatgccgac tgttcgccca cgctcaacgc gcgcatcagc
     4981 ccgatgccgt ctgaaagccc gccgtccgca tccgcctgct gccaattacg gctgatgcgg
     5041 ctgcgccaaa ccgcaatccc ccaaccgctt ccgccatgca gcaaaaccct gtccgcaccg
     5101 accgtccccg caccgcctat tccgttggat aatgcccacg cctcacggtt caaaccgcgc
     5161 aggttcaact ccccgacgac agggtgcaca cgtgccgtta tccgccatct gctcccgacc
     5221 gcccattcgc gccgtttgta gtccgacagc aacaaatcaa acgtccgacc accgctgtcc
     5281 acagcctttg ccgcaaactg cacacgccgc ccgtccgacc tcggcatatc cgccacttcc
     5341 accgtcaacg gcacacctga aaccgcctcc gcccgccatt gcgaagacag tgccgcttcc
     5401 gttctgaata cgccgtaagc cgcgcccgcc aacacgcaca gcatcagacc ggcaaacgca
     5461 aaacgccttg caaacacagc aaaaaccgca aaagccgcca gccaaaacag ccaatgcggc
     5521 acgaccggca gcgcaaacga agccgccgcg ccgacaaccc aacacggcaa aaaccaacga
     5581 cggaacacgt ctccccaccc cagatatttc aaaaataaac ggataataaa gcaaatccga
     5641 aaaaccgtca taacaagatt tgaaaatgat atagtggttt aaatttaaat caggacaagg
     5701 cgacgaagcc gcagacagta caagcagtac gacaaggcga ggcaacgctg tactggtttt
     5761 tgttaaacca ctataagaat gcaaacgaaa agcctcagta ttatccgaga caacggcaaa
     5821 ctttccatcg tcattcccac gaacccacat cccgttatta ccacgaaagc gggaatctag
     5881 gactcagggt taagaaaacc tacatcccgt cattccctca aaaacagaaa accaaaatca
     5941 gaaacctaa
//
LOCUS       DAXHTR010000128         5811 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.128, whole genome shotgun sequence.
ACCESSION   DAXHTR010000128 DAXHTR010000000
VERSION     DAXHTR010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.128"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(231..386)
                     /gene="rpmG"
                     /locus_tag="ACFNML_001687"
     CDS             complement(231..386)
                     /gene="rpmG"
                     /locus_tag="ACFNML_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFC6393029.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(418..651)
                     /gene="rpmB"
                     /locus_tag="ACFNML_001688"
     CDS             complement(418..651)
                     /gene="rpmB"
                     /locus_tag="ACFNML_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFC6393030.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(901..2427)
                     /gene="farB"
                     /locus_tag="ACFNML_001689"
     CDS             complement(901..2427)
                     /gene="farB"
                     /locus_tag="ACFNML_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HFC6393031.1"
                     /translation="MDYPPLKGAALAWITLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     SLVIFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPILGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGVIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPIIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGAGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSFVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     QGISDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(2451..3623)
                     /gene="farA"
                     /locus_tag="ACFNML_001690"
     CDS             complement(2451..3623)
                     /gene="farA"
                     /locus_tag="ACFNML_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HFC6393032.1"
                     /translation="MATHADETTIQNRQEANSAKVKRKRRLTALTLLFALAAAAAGSA
                     FFLWWQHEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVL
                     AYERAKNELVQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSA
                     EELAHARAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQ
                     RTQIRAPADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPA
                     ELVSDLYGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDV
                     DRHPLRIGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     gene            complement(3667..4026)
                     /locus_tag="ACFNML_001691"
     CDS             complement(3667..4026)
                     /locus_tag="ACFNML_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6393033.1"
                     /translation="MINLELKAINLYDCAFEDFVPEIKDNFCVGIDLDIGTDDSDAAD
                     IFSVRICSPKWILHNCFQNQRVKWGAGMMIMNEFNHSVIKSEIEKILKECSKETWEKS
                     LTYLLRFFSWEFEDYQC"
     regulatory      4241..4285
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            4289..4762
                     /gene="queF"
                     /locus_tag="ACFNML_001692"
     CDS             4289..4762
                     /gene="queF"
                     /locus_tag="ACFNML_001692"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046857 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors, with NAD or NADP
                     as acceptor [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HFC6393034.1"
                     /translation="MSRNTEELQGISLLGNQKTRYPTGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIYIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            4839..5525
                     /locus_tag="ACFNML_001693"
     CDS             4839..5525
                     /locus_tag="ACFNML_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFC6393035.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYIFSVLFHNGSWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFNKLRRLKAWWIAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIALVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT         1462 a         1613 c         1326 g         1410 t
ORIGIN      
        1 gtattattat agtggattaa caaaaatcag gacaaggcga cgaagctgca gacagtacaa
       61 atagtacgga accgattcac ttggtgcttc agcaccttag agaatcgttc tctatccttg
      121 gcagacggtt tgcagcagat tcgcatacgg cagatgtttc aagcggaaaa gaaacacgat
      181 aaaaataaaa aagcctccga acagtcggag gctttgcttt caaactgaaa ttatttcagt
      241 ttggtttctt tgtacactac gtgtttgcgg gcaaccggat cgaatttttt aatttccagt
      301 ttgccgggca tagtgcgttt atttttggta gtggtgtaga agtgaccagt acctgcactg
      361 gattccagtt tgattttatc gcgcattgca gtaatcctta attaaatagt gtttaaatta
      421 agcttcgccg cgagcacgca aatcagccaa tacgacatca atgcctactt tgtcgatggt
      481 acgcagtgca gcgttggaaa cgcgcaggcg aacccagcgg ttttcacttt ctacccaaaa
      541 acgacgtgat tgcaagttgg gcaaaaaacg gcgtttggtt ttgttgttgg cgtgcgatac
      601 gttgttgccg gacatcgggc gtttgccggt cactttgcaa actcgtgcca taatcagtct
      661 ccaaacttct aaaatagtaa aacgcgattt ataccacgaa aaacagtgtt agttcaagtg
      721 ttttaaagaa aaacggctgt tttgcgcgga taatccttcc gattgtcgca tctgtggaac
      781 gtttgcaggt gcgaaacaga tggggcgcga ttatagcgga tttcgggaac tgctgcattc
      841 aaaatgccgt ccgaacatat ttccagtatt ttcagacggc atttcaagtt ttcaaatccc
      901 tcaatgtcca ccgccgccgc cgttgtggaa cggcggtttt gccagccata tgacaggaat
      961 catgatgatg aacagcaggc tgcctgccat aaagatttca ttcgatccga tgatgaagcc
     1021 ctgctgggta atggtattgt tgatgatacc tagggtttga atgtcggaaa tgccttgttg
     1081 ggacaaatgc gccgccgttt cgtgcaatgt tgcggaatag ggcgtgatgt gttcggcaaa
     1141 gcgtgtgtgg tgcaacgctt cgcgccgttc ccacagggta ctgacgaatg atacgccgac
     1201 accgcccatc agcacgcgca agaaattcga caggctgccg gcggcggcaa tctgtccgcc
     1261 cttcatatgc gacagtgtga tggtggtcag cggcaggaag aacatggcaa cgcctgcccc
     1321 ctgccaaaac tgcggccaga tgacgttgcc gatatccatg tcggcataaa aatcggtgcg
     1381 ccaatagaaa gtgaaggcaa aagtcaggaa gctggcggtt acgagcaggc gcatatcgat
     1441 tttattgccg aacctgccga taatggggga caggaaaact gggaggatgc cgacgggcgc
     1501 ggcggcaagc cctgcccacg tggaggtata gcccaggttg gtctgcaaca ctaacggcag
     1561 cagggtcagc gtccccatat acaccataaa acccaatgac gtggcaatga cgccgacggt
     1621 aaaattccga tctttaaaca gcgataaatc gacaatcgga tatttttctc ccaattccca
     1681 aacaataaaa tacgacaagc acaccaatgc ggttacgccc aaggtaatga tttctcccga
     1741 agcgaaccag tcgagttcct tacccctgtc cagcatcatc tgtaacgcgc cgataccgac
     1801 taccatcaat gtaagcccga catagtcggt cggcatttta acggtttccg tttcccgata
     1861 tttcaaatgt ttccatgtaa tccatgccga tatgataccg atagggatat taatgaagaa
     1921 aatccaaccc caatgccagt ttccggaaat ccaaccgccg agtatcggcc cgagaacagg
     1981 ggcgacaacg acggtcattg cccacaacgc cagtgccagc gtccgttttg cgggcggata
     2041 ggatgccatt aacaggcttt gcgacaaggg aatcagcggc ccggcgataa agccctgcaa
     2101 gatgcggaaa ataaccagcg actgaaggtt gggcgcgatg ccgcacagcc acgatgtgat
     2161 gacgaaaccg acagcggcgg cggtaaacaa tttgacctcg ccgatacgtt ttgccaaaaa
     2221 gcccgttaac ggcacggaaa cggcgtttgc cacagcaaaa gaagtaatga cccacgtccc
     2281 ctgagtggtt gccgcaccga ggttgccggc gataacggga acggcgacat tggcgatagt
     2341 cgtatctaaa acttccataa atacggcaag ccccaaagac agcgtaatcc atgccaatgc
     2401 cgcaccctta agcggtggat aatccattgt gtcctccgtt tcagacggca tcagggcgcg
     2461 gattgcccga ggatttcatc gaccgtccga tcgacttccg accagtcggt actttccatt
     2521 tccggcaatg ccgcacccgg cgtttttgaa acaggcgcgc ctgcggcgga agtatccact
     2581 ttaaccgtca tcgacaaacc gatacgcaac ggatgcctgt ccacatcttc gcggttcagc
     2641 acgatacgga cggggacgcg ctgcaccact ttaatccagt tgcccgttgc gttttgcgcc
     2701 ggaatcagcg aaaacgcgct gcccgtacct gccgaaaaac ctgccaccct gccgcgataa
     2761 acaatttgtt tgccgtacaa atcggacacc agctcggcag gctgtccgat tttcatatgc
     2821 cgcaactgcg tctctttaaa attggcatcc acccacacat ccgacagcgg caccaccgcc
     2881 atcagcggcg cgcctgccgc cacctgctgc ccgacctgca ccgaacgctt cgccacctga
     2941 ccgtccgccg gcgcgcggat ttgcgtccgc tgaaggttca accacgcatc tttcaacctg
     3001 ccgattgccg tctgaaccgc cggctgttcg cgcaaagaaa cctgaccgcc caaagccgcg
     3061 cgtgccgaag actcttccgc caaagccgcc ttaactgccg cctgcgcctg agacactgcc
     3121 gcacgggcgt gtgccagctc ttcggcggac accgcgcccg attccgccaa agcagaccgg
     3181 cggcgcaaat catcctgcgc gcgtgccaaa tccgcacggc gcaaggcaac ctgcgccccc
     3241 gcctgcgaag tggcggcatt ttgccggcgg ttttgccgca ccgcctgaac cagctcgttt
     3301 tttgcccgct cgtaagccag cacatcatta tcgtcgtcca ataccgccag cacgtcgcct
     3361 tttttcacgg catccgtatc gtcgtgcaaa accttccgta ccgtaccgcc cttttgcggc
     3421 gtaacctgaa ccacgcgtcc ggcaacataa gcgtcttccg tttcctcttc gtgctgccac
     3481 cataaaaaaa acgccgaccc ggcggctgcg gcggcaagcg cgaacagcag cgtcaatgcc
     3541 gtcaggcggc gtttacgttt gacttttgcg ctattggctt cttgtctgtt ttgaattgtc
     3601 gtttcatctg catgtgttgc cattattcca attccaaata cttctaatac tatgtttttg
     3661 atttaattag cactgataat cttcaaattc ccacgaaaaa aaacgaagta aataagtcaa
     3721 tgacttttcc caagtttctt ttgaacattc tttaagaatt ttctcaattt ccgatttaat
     3781 aacagaatga ttaaattcat tcataatcat catacccgct ccccatttaa ccctttgatt
     3841 ttggaaacaa ttatgcaaaa tccatttagg agagcatatg cgaacagaaa atatatctgc
     3901 agcatcacta tcatcagttc ctatgtctaa atcaattccc acacaaaaat tgtctttgat
     3961 ttcgggaacg aaatcttcaa aggcacaatc gtaaagattg atggctttca attctaggtt
     4021 aatcatttta tattcaaaat aaataaaacc aattaaagat tggttaggat tctataccct
     4081 ggcaataact caatcaagtt aatgatttag aatgaatatt caaataaact gtatcagccc
     4141 ggcctcacag aactatttat atccacacat caccgagtaa agcaattacc catactaacc
     4201 gaagaaaact acacaaatac cgccacttat attacaatcg ccgcccgtgg ttcgaaaacc
     4261 tcccacacta aaaaactaag gaaaccctat gtcccgcaat accgaagagc tgcaaggcat
     4321 ctcgcttttg ggtaatcaga aaacccgata tccgaccggc tacgcgcctg aaattctcga
     4381 agcattcgac aacaaacatc ccgacaacga ctatttcgtc aaattcgtct gcccagagtt
     4441 caccagcctc tgccccatga ccgggcagcc cgacttcgcc accatctaca tccgctacat
     4501 tccgcacatc aaaatggtgg aaagcaaatc cctgaaactc tacctcttca gcttccgcaa
     4561 ccacggcgat tttcatgaag actgcgtcaa catcatcatg aaagacctca ttgccctgat
     4621 ggatccgaaa tacatcgaag tattcggcga gttcacaccg cgcggcggca tcgccattca
     4681 tcctttcgcc aattacggca aagcaggcac cgagtttgaa gcattggcgc gtaaacgcct
     4741 gtttgagcac gacgcacaat aaaccatcct tcccatattt cagacggcat tccttcgata
     4801 cgatatgccg tctgaacgcc tttccgaaag gtttttttat gtacgcattt accgacgcac
     4861 agcaacagaa ggcactcttc tggctggtgc tttttcatat cctcatcatc gccgccagca
     4921 actatctggt gcagttcccc ttccaaattt tcggcatcca caccacttgg ggcgcatttt
     4981 cctttccctt catcttcctt gccaccgacc tgaccgtccg cattttcggt tcgcacttgg
     5041 cacggcggat tatcttttgg gtgatgttcc ccgccctttt gctttcctac atcttttccg
     5101 ttttgttcca caacggcagt tggacaggct tgggcgcgct gtccgaattc aacacctttg
     5161 tcggacgcat cgccttagcc agctttgccg cctatgcgct cggacaaatc cttgatattt
     5221 ttgtattcaa caaattacgc cgtctgaaag cgtggtggat tgccccaacc gcatcaaccg
     5281 tcatcggcaa cgccttggat acgttggtat ttttcgccgt tgccttttac gccagcagcg
     5341 atggatttat ggcggcaaac tggcagggca tcgccttggt cgattacctg ttcaaactca
     5401 ccgtctgcgg tctgtttttc ctgcccgcct acggcgtgat tctgaatctg ctgacgaaaa
     5461 aactgacgac cctgcaaacc aaacaggcac aagaccgccc cgcgccctcg ctgcaaaatc
     5521 cgtaaaacag ccctgccggg agtcgggacg gttgggtttt cttttatttg caaacactgt
     5581 cggaacggat aaatccggat tcccgcccac ttcgtcattc ccgcgaaagc gggaatccgg
     5641 tgcgttgggt ttcagtcatt tccgataaac tcctgccgcg ttgtatttct agattcccgc
     5701 tttcgcggga atgacggcgg atgggtttcc gtgccgtggt gtccggattc ccgcccgtac
     5761 gggaatgacg gcagtgcttc cttggcttaa ttttatttta atcgactgta t
//
LOCUS       DAXHTR010000129         5792 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.129, whole genome shotgun sequence.
ACCESSION   DAXHTR010000129 DAXHTR010000000
VERSION     DAXHTR010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.129"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(129..890)
                     /locus_tag="ACFNML_001694"
     CDS             complement(129..890)
                     /locus_tag="ACFNML_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393036.1"
                     /translation="MDVNKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRDHVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMALFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            990..1241
                     /locus_tag="ACFNML_001695"
     CDS             990..1241
                     /locus_tag="ACFNML_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393037.1"
                     /translation="MGSIEQRLEYLEEANDVLRMQNHVLSTAFKALIRALPAETAEIA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            1293..2099
                     /locus_tag="ACFNML_001696"
     CDS             1293..2099
                     /locus_tag="ACFNML_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HFC6393038.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSEDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALKKVRESGEYDKIYAKYFAKEDGQAAK"
     gene            2406..3929
                     /locus_tag="ACFNML_001697"
     CDS             2406..3929
                     /locus_tag="ACFNML_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HFC6393039.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGDKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRIDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLDKGEELPVDFTNRLIYYVGPVDPVGDEVVGPTG
                     PTTATRMDKFTRQMLEQTGLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVES"
     gene            4027..5439
                     /gene="trkA"
                     /locus_tag="ACFNML_001698"
     CDS             4027..5439
                     /gene="trkA"
                     /locus_tag="ACFNML_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFC6393040.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
BASE COUNT         1464 a         1719 c         1408 g         1201 t
ORIGIN      
        1 cggcttcgtc gccttgtcct gatttttgtt aatccactat attaatattt tgcgcccgtt
       61 ccgtgaggct ttcaagtcgg atgagaatgc aaatgccgtc tgaacacggc tttcagacgg
      121 catgacggtc agcgtttgta ttttaactcg tacttgatgt cgttgaggat tttgcggaca
      181 tcgtgttcca acacgtcttc gaccaccgcg cccgcctgct cgtgcagcat ttgctggagc
      241 tgataagtaa agagtgccat ctgcttttgc accgccgttc gaatgatgcc gttgacggta
      301 tcggtcagat gcggacgcag gcgtttgatc agccgttcgg tcagctcctg ttcggacagg
      361 cagaacactt cgcgccggtt gacggctttc gggttcagga tattgatttg gacgggcatc
      421 aacgcctctt ccgcaccgcc ttcgccgttt tccgcaaccc cgtctgccgc cgtacccgat
      481 tctgccgcat cggcgttttc cccgccttca atctgtccgg tttcaaattc ggcactgtct
      541 tttttggcat cgaacaggat tctccgccgc gattcgatgt gtttttccga aaccgacatt
      601 tgcagggaag cctgcgcgtt gagccagttt tcctgaagga caatcatcgg gtcggtttcg
      661 acttcctcgc cgcaatcggc aacggcggca ttgtgttcct cctgccattt tttcagatac
      721 gccttcaaca catggtctcg gctctcatcg tccagtttcg gcacaggcgc gtccgttccg
      781 gtttcagagg ggcgggacag cggcgcgtaa gtcggcactg ccttcatacg gcgcgtctga
      841 cgcaggtttt ccaaacgttt ttcccaattc ggctctttat tcacatccat tttcggcttc
      901 cggttcttaa tctttgcagg cagacaaacc cgcgcccaaa gcgcggtttg atataatggc
      961 gcattttaac agattcgcga ggatacatca tgggcagcat cgaacagcgt ttggaatatc
     1021 tggaagaggc gaacgacgtg ctgcgtatgc agaaccacgt cctgtccacc gcattcaaag
     1081 ccttaatccg cgcccttccc gccgaaaccg ccgaaatcgc ggtcgagtcg attcagcttg
     1141 cttttgagga cgccttggca gaattgagct atgaggacag cccccatacc gatttgttcc
     1201 acgacgttac ttatgcgttt ttccgtgaaa aagaacgtta attttatgtt aaactgattt
     1261 tttaggcttt ttgattaccg aaaggaattt tgatgaatat gaaaaaatgg attgccgccg
     1321 cccttgcctg ttccgcgctc gcgctgtctg cctgcggcgg tcagggtaaa gatgccgccg
     1381 cgcccgccgc aaaccccgac aaagtgtacc gcgtggcttc caacgccgag tttgccccct
     1441 ttgaatcttt agactcgaaa ggcaatgtcg aaggtttcga tgtggatttg atgaacgcga
     1501 tggcgaaggc gggcaatttt aaaatcgaat tcaaacacca gccgtgggac agccttttcc
     1561 ccgccttgaa caacggcgat gcggacgttg tgatgtcggg cgtaaccatt accgacgacc
     1621 gcaaacagtc tatggacttc agcgacccgt attttgaaat cacccaagtc gtcctcgttc
     1681 cgaaaggcaa aaaaatatct tcttccgaag atttgaaaaa catgaacaaa gtcggcgtgg
     1741 taaccggcta cacgggcgat ttctccgtat ccaaactctt gggcaacgac aacccgaaaa
     1801 tcgcgcgctt tgaaaacgtt cccctgatta tcaaagaact ggaaaacggc ggcttagatt
     1861 ccgtagtcag cgacagcgcg gtcatcgcca attatgtgaa aaacaatccg accaaaggaa
     1921 tggacttcgt taccctgccc gacttcacca ccgaacacta cggcatcgcg gtacgcaaag
     1981 gcgacgaagc aaccgtcaaa atgctgaacg atgcgttgaa aaaagtacgc gaaagcggcg
     2041 aatacgacaa aatctacgcc aaatattttg caaaagaaga cggacaggcc gcaaaataag
     2101 cccgcccatc cgaacacaat gccgtctgaa gccctttcag acggcattgt tcatcaatcg
     2161 gcctacaatg aactgcctgc tgatttctcc ctaccgcaaa gcaacaggca aagattacaa
     2221 atatcaaaat ccgagtaaaa cagtatttta ttaaaacaaa ttgataatca agagattaga
     2281 attatgtatt gtctttaccg tacaaacgct ggcactattt caacctgata aaaaacagcc
     2341 ttcaaaaagg ttgcttaaaa caacagcaga cacttaccgc cacaaccttg aaaaggaaca
     2401 caatcatgac cgtcatcaaa caggaagact ttatccaaag catttgcgat gccttccaat
     2461 tcatcagcta ctaccatccc aaagactaca tcgacgcgct ctataaagcg tggcagaaag
     2521 aagaaaaccc cgccgccaaa gacgcgatga cgcagatttt ggtcaacagc cgcatgtgtg
     2581 ccgaaaacaa ccgccccatc tgccaagaca caggtatcgc aaccgtgttt ttgaaagtcg
     2641 gtatggatgt gcagtgggat gcggacatga gcgtggaaga aatggttaac gaaggcgtac
     2701 gccgcgccta cacttgggaa ggcaatacgc tgcgcgcttc cgttctcgcc gacccggccg
     2761 gcaaacgtca aaacaccaaa gacaacacgc ccgccgtcat ccatatgagc atcgtgccgg
     2821 gcgacaaagt cgaagtaacc tgcgcggcaa aaggcggcgg ttctgaaaac aaatccaaac
     2881 tcgccatgct caatccttcc gacaacatcg tcgattgggt attgaaaacc atcccgacga
     2941 tgggcgcggg ctggtgtcct cccggcatct tgggcatcgg catcggcggc acgcccgaaa
     3001 aagccgtgtt gatggcaaaa gaatccttga tgagccacat cgacattcaa gagttgcagg
     3061 aaaaagcagc gtcaggtgca gaattgtcca ccaccgaagc cctgcgcctc gaactctttg
     3121 aaaaagtcaa cgcgctgggc atcggcgcgc aaggcttggg cggtctgacc accgtgttgg
     3181 acgtgaaaat cctcgattac ccgacccatg ccgcttctaa gccgattgcg atgattccca
     3241 actgcgccgc cacccgccac gtagagtttg aattggacgg ctcaggccct gtcgagctga
     3301 ctccaccgcg cgtcgaagac tggcccgatt tgacttacag ccccgacaac ggcaaacgca
     3361 ttgatgttga taagctgacc aaagaagaag tggcgagctg gaaaaccggc gacgtattgc
     3421 tgttgaacgg caaaatcctc accggccgcg atgccgcaca caaacgcctc gtcgatatgc
     3481 tcgacaaagg cgaagaattg cccgtcgatt tcaccaaccg cctgatttac tacgtcggcc
     3541 ctgtcgatcc ggtcggcgat gaagtcgtcg gcccgacagg ccccaccacc gccacccgta
     3601 tggacaaatt cacccgccaa atgctcgaac aaaccggcct cttgggtatg atcggcaaat
     3661 ccgagcgcgg cgcggccacc tgcgaagcca ttgccgacaa caaagccgtg tacctcatgg
     3721 cggtcggcgg cgcggcgtat ctcgtggcaa aagccatcaa atcttccaaa gtcttggcgt
     3781 tccccgaatt gggcatggaa gccatttacg aatttgaagt caaagacatg cccgtaaccg
     3841 tcgccgtgga cagcaaaggc gaatccatcc acgccaccgc cccgccccaa tggcaggcga
     3901 aaatcggcat catccccgtc gaatcttgag gcgccatgcc gtctgaacac aaaatctgct
     3961 ttcagacggc atttccgccc ccggttgcgg tacaatccac catttcatca ctcggcgacc
     4021 cacaccgtga aaatcctcat tttaggcaac ggacaggtag gttctaccgt cgcacaaaac
     4081 cttgccgcca tacccaacaa cgacgtaacc gttatcgaca tcgacgaaaa agcattgcag
     4141 gaaacaggca gccgcctcga tgtccaaacc gttttcggca acggcgcatc ccccttcaca
     4201 ttagaacgcg ccggcgcgga agatgccgac ttgctgctcg cgctctcccg cagcgacgaa
     4261 accaacatcg tcgcctgcaa agttgccgcc gacctgttca acatccccgg ccgcatcgcg
     4321 cgcgtccgtt ccagcgaata cctcgaatac ctcagcccca agctcgaaaa caacgaaaac
     4381 ggcagccttt ccatattcgg cataaccgaa accatcagcc ccgaacagct cgttaccgaa
     4441 cagcttgccg gcctgataga ctgcccgggc gcattgcagg ttttacgttt tgcagacgac
     4501 cgcgtgcgga tggtcatcat acaggcgcgg cgcggcggac tgcttgtcgg acgcagcatt
     4561 gccgacatcg cccaagattt gcccgacggg gccgactgcc aaatctgtgc cgtttaccgc
     4621 aacaaccgcc tcatcgtccc cgcgccgcaa accgtcatca tcgaaggcga cgaaatcctg
     4681 tttgccgccg ccgccgaaaa catcggcgcg gtcatacccg aattgcgccc caaagaaacc
     4741 agcacccgcc gcatcatgat tgccggcggc ggcaacatcg gctaccgtct cgccaagcag
     4801 ctcgaacacg catacaacgt caaaatcatc gaatgtcggc cgcgccgtgc cgaatggata
     4861 gccgaaaacc tcgacaacac cctcgtcctg caaggttcgg caaccgacga aaccctgctc
     4921 gacaacgaat acatcgacga aatcgacgta ttctgcgccc tgaccaacga cgacgaaagc
     4981 aacattatgt ccgccctttt ggcgaaaaac ctcggcgcga agcgcgtcat cggcatcgtc
     5041 aaccgctcaa gctacgtcga tttgctcgaa ggcaacaaaa tcgacatcgt cgtctccccc
     5101 cacctcatca ccatcggctc gatactcgcc cacatccggc gcggcgacat cgttgccgtc
     5161 caccccatcc ggcgcggcac ggcggaagcc atcgaagtcg tcgcacacgg cgacaaaaaa
     5221 acttccgcca tcatcggcag gcgcatcagc ggcatcaaat ggcccgaagg ctgccacatt
     5281 gccgccgtcg tccgcgccgg aaccggcgaa accattatgg gacaccatac cgaaaccgtc
     5341 atccaagacg gcgaccacat catctttttc gtctcgcgcc ggcgcatcct gaacgaactg
     5401 gaaaaactca tccaagtcaa aatgggcttt ttcggataaa ccgccccatt ccggacacat
     5461 tgccgccaag cggtatggaa gcagaaataa tggtaggtgg gcttcagacg gcatccgccc
     5521 tccccgtcat tcccgcgcag gcgggaatct agaccttggg atagcggcaa tattcaaagg
     5581 ttatctgaaa atttagaggt tctagattcc cgttttcacg ggaatgacga agagttgcgg
     5641 gaatctagac cattggaaag cggcaatatt caaaggttag ctgaagcttt agaggttcta
     5701 gattcccgtt tccacgggaa tgacgaaagg tggcgggaat ccagaacgtc gggcaacggc
     5761 aatattcaaa agccgtctga aaatttaaaa gt
//
LOCUS       DAXHTR010000130         5791 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.130, whole genome shotgun sequence.
ACCESSION   DAXHTR010000130 DAXHTR010000000
VERSION     DAXHTR010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.130"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            132..566
                     /locus_tag="ACFNML_001699"
     CDS             132..566
                     /locus_tag="ACFNML_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HFC6393041.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPLNDFQPNCD
                     IRRLGLTQGQHNELRKIRAAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            complement(579..1442)
                     /locus_tag="ACFNML_001700"
     CDS             complement(579..1442)
                     /locus_tag="ACFNML_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFC6393042.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYANKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(1450..2067)
                     /locus_tag="ACFNML_001701"
     CDS             complement(1450..2067)
                     /locus_tag="ACFNML_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFC6393043.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFQTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKSNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            complement(2230..2844)
                     /locus_tag="ACFNML_001702"
     CDS             complement(2230..2844)
                     /locus_tag="ACFNML_001702"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HFC6393044.1"
                     /translation="MKTSNFSKRTSAMKTFALILAGGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPDDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARSVRFEFDGHFADLNTQTDLQEG"
     gene            3050..4822
                     /locus_tag="ACFNML_001703"
     CDS             3050..4822
                     /locus_tag="ACFNML_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HFC6393045.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRAQIDNQVAEFEKSLKRIAQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGITVETAWENGSFLPPQEAQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIHAV
                     LEIRSQPQQGTTVSLTGASEESLK"
     gene            4819..5475
                     /locus_tag="ACFNML_001704"
     CDS             4819..5475
                     /locus_tag="ACFNML_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HFC6393046.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHGFEVIGEAADGLSGIKMI
                     SRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDSDDLTECIRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPMLE
                     "
BASE COUNT         1428 a         1636 c         1344 g         1383 t
ORIGIN      
        1 gttccgattc cgccgttatg tctgcctgcc ggacatacgt acgcattatt aacaaaagtt
       61 aaccgcgata ataccatctt tcacacgtca atctagtata tttcctaaaa tttccaacaa
      121 gaggaaaagc cgtgccactg cctgctccct gccgttttgc caaacctgcc gcctcttttt
      181 taagtatggc tttgctttcc tgccagcttt cccacgccgc cacggcttat atccccctga
      241 acgattttca accgaactgc gacatacgcc ggctcggact gacacagggt cagcacaatg
      301 aactgcgtaa aatccgcgcc gccttcaaaa tggcgggcga cagggcgcgt ttgaaggtta
      361 tgcattccga acacagccgc cgccggtctg tcgtcgaaat catttcctcg gatgttttta
      421 atcggaacga ggcgcgcgat tatgtcgaaa gccgctattt gtccggtatg gattttgcgg
      481 tggacgaatt ggaaatccaa caccggttct tccatatcct cacaccgcaa cagcagcaaa
      541 tgtggctttc ttcctgcctc aaataatccc cgaaacgctc acaacgcccg ttgtttcggc
      601 agcctgcccg cccagtcgca ggcaaactgc cacgcggaac gtcccgaacg gctgccccgc
      661 gtctgcgccc actgcaatgc cgccatctgt gcggtttcat catacggcac gccgaaatct
      721 tccagccaat tttgcaccgc cgccagataa tcgttttgat cgaacggata aaaactgagc
      781 cacaatccga atcggtcgga cagggatatt ttttcttcca ccgcttcttt ctgatggatt
      841 tccccccgca tccccgtcgt accggcattc tcgtcaaaat attcgggcat cagatgccgt
      901 ctgttggaag tcgcgtaaac caaaacgttg gcgcaacgtt gagacagacc gccgtctaac
      961 gcggttttca atgccttata ggtttcatcg ccgctttcaa acgacaaatc gtcgcaaaat
     1021 acgataaatt tttccggacg ttccttcaaa agcgtcaaca ggtaaggcag gccgattaaa
     1081 tcgcttttat cgacttcgat caggcgcaat cccttattcg catattcgtg tagcagggct
     1141 ttgaccagcg aggatttgcc tgttccgcgc gcgccgctca tcaatacgtt attcgcgggt
     1201 ctgccgacaa tgaactgttc ggtattacgc accagcaatt cggtttgcct gccgactccc
     1261 gccagcctta ccaagggaaa ggtgtgcgga tcgggcaagt gttccaaaaa accttttttg
     1321 cccgcactct gccagcggaa ggcaagcgcg ttccaatccg tatgcccggg ttcgggcgga
     1381 agcacggcat ccaaacgccg caaaacggca taggctttat cgagaaattc gttcaattcc
     1441 atctctacct cactttgcat atctttgcgc catcagccgt tcgacggtgt cgacgattgc
     1501 ctgcgtattc ggatcgattt cgatgttgat cctgtcgccg acctttctgc tgccgaacag
     1561 cgtccgttcc aaagtttcgg gaatcagatg gacattgaaa cggccgtctt cgactttgcc
     1621 tatggtcagg ctgcaaccgt ccaagccgac gaaccctttg gtcaggatat agggtttgag
     1681 ttcatgcggg agcgaaaacc aaaccgtgcg gttgaacccg tcccgttcga tttcgacaat
     1741 aggcacggtt gccataatgt gtccgctcat gacgtgtccg ccgatttcgt ctccgaaacg
     1801 cgccgcccgt tcgatgttga cgcaatcgcc ttccttcagc agccccaaat tgctttttgc
     1861 caaagtttcc gccattaaat cgaagctgac gcggtttcct tcgatttcgg taatcgtcag
     1921 gcagcagccg ttattggcaa ccgatgcgcc gcgttgcaga ttgtccgccg cctcttgcgg
     1981 aagctcgacg acataagtct gaaatgcctc cgacgggcgg tggattgccg tcagttttcc
     2041 caatccttga acaatgcctg taaacataat cctgtttccc tgtgtcggta aaaatggtgc
     2101 aaattgtagc atctccccgc gaaaaatgcc gtctgaaatg ccttcagaca gcattatgcc
     2161 tccgattcgg gcaaaaaccg cccggtatgg cttgaccttt cctttccacg ccggtcggcg
     2221 gtcttgccct tatccctcct gcaaatcggt ttgcgtgttc aagtcggcaa aatgcccgtc
     2281 aaactcgaat ctgaccgacc gcgccctttg ctgctgcaac cagcttctta atgttttcat
     2341 acccgaaaac agatagggaa tcgcgctttg cagaatctgc gggcggatat acataatgtt
     2401 gtagtgcatc gttatcggcg tttccacata atacgcattg cacaacggtg tgcgtttcga
     2461 cacggtttca aacctcgcca ccaaatcgtc cggcagatac ggcatatcgc acggcacaac
     2521 caaaagccag tcagcagtcg ccaactgcaa atcgttggct gcggtacaca atgccgaaag
     2581 cgggccgaaa tgctgccact gccgcgcatc gggaaaaata tgcggacttc ttcgagcata
     2641 ttcttccaaa ttccggttgg tgctgatggc gatatggctg acctgcggcc tgaccctgtc
     2701 gatgacatgg tctatcagtg ccttaccccc caaaagagca agccccttgt cctcgcctcc
     2761 catacggctc gcctgaccgc cggccagtat cagggcaaaa gttttcattg cggatgttct
     2821 cttggaaaag ttcgaggttt tcatgattgc agtctgccgt tcccaatgac tcaaaatgcc
     2881 gtctgaagca gacggcaaat aaattcatat tatctgaatt ttatcataac atgatttaac
     2941 actgaagcgg tgcggattaa gttttcctta accaattctt tctgagcagt tgtatctaat
     3001 tccaaagaat gatattgttt gcattattta gaacaatttt tcgccgagca tgatactgcc
     3061 agcccgtttt tcagacggca tcagcctttc cctgcgcctg aaactcctga ccggactgtg
     3121 ggtcgggttg gcggcattgt ctgtcgtttt gacactgctg ctctctttgc gtctggaaaa
     3181 cgcggcctcc gtcatcgaag aggcgggcaa cttgagaatg caggcatacc gtctggcata
     3241 catggcgggt gaaggctcgc cccgtgcgca aattgacaat caggttgccg aatttgaaaa
     3301 aagtttaaaa cgcattgccc aaagcgatgc catccatccg ctgattcctt cggacacccc
     3361 tcttgcttat gatttgatac aatccatgct gattatagat tggcaggcac acatcctccc
     3421 cccgctccag tcctaccggc gaccgactca ggtcgatctc taccgctttg ccggaaacat
     3481 cgaactgttt ttgcaggcat tggaaaatgc caacgaaaaa aacacatggt ggctcaggcg
     3541 ttttcaatgg gcaattatgt tgatgacgct ggtgtcgtct gtactgatgc tgttttggca
     3601 ccagatttgg gttatccggc cgctgcaggc gttaagggaa ggtgcggaac gcatcggacg
     3661 gaggtgtttc gatattccgg ttcccgaagg cggtacgccg gaattcaaac aggtcgggcg
     3721 ttgtttcaat caaatgggcg gcaggttgaa aattttatat gatgatttgg aaggacaagt
     3781 cgccgagcag acacgcagtc tcgaaaaaca aaatcaaaac ctgaccctgc tgtaccaaac
     3841 tacacgggac ctgcaccaat cctacatacc gcaacaggct gcagaacatt ttctaaaccg
     3901 tatcctgccc gccgtaggag cagattccgg cagagtttgt ttggacggcg gatccgatgt
     3961 ttatgtttcc attcatcatg cggattgcgg cacagcagct tcggatttgg ggaagtacca
     4021 tgaggaaatc ttccccattg agtaccagaa cgaaacattg ggcaggctgt tgctcagctt
     4081 tccaaacggc atttctcttg atgaagacga ccgcatcctg cttcaaacac taggcaggca
     4141 attgggcgta tcgcttgccg gcgcaaaaca ggaggaagaa aaacgcctgc ttgcagtatt
     4201 gcaggaacgc aacctgattg cgcaaggatt acatgacagc atcgcacaag cattaacgtt
     4261 cctaaaccta caggtacaga tgctggaaac cgcctttgcc gaaaacaaac gggaggaagc
     4321 cgcagaaaac atcggcttca tcaaaacagg cgtgcaggaa tgttatgaag atgtccgcga
     4381 actgctgctc aacttccgta ccaaaatcag caataaagaa tttcccgaag ccgttgccga
     4441 cctattcgcc cgctttacgc aacaaaccgg gataacggtc gaaaccgcct gggaaaacgg
     4501 ttcgttcctg ccgcctcagg aagcgcagct ccaaatgatt tttatcctgc aggaaagcct
     4561 gtccaacatc cgcaaacacg cccgcgccac ccatgtaaaa ttcacccttt ccgaacacgg
     4621 cggacgcttt accatgacca tccaagacaa cggacaaggt ttcgacacgg agaaaatagg
     4681 agaacctacg ggcagccatg tcggactgca catcatgcag gagcgtgcca agcgcatcca
     4741 tgccgtttta gaaatccgtt cccaacccca acagggaacc accgtctcat tgacgggtgc
     4801 atctgaagaa agcttgaaat gaccattaaa attattctga tagacgacca caccctcttc
     4861 cgcagcggca ttaaagccct tttgtcgcgc caacacggtt ttgaagtcat cggcgaagcc
     4921 gcagacggcc tctcgggcat caaaatgatt agtcggctgc aacccgatgt cgtcctgctc
     4981 gatttggata tgcccggcat gaacggacgc gaagcactct cccaaatcat cagcatcaat
     5041 ccgcagcagg cagtgattat gctgaccgtt tccgaagaca gcgacgactt gaccgaatgt
     5101 attcgcatcg gcgcgcgcgg ttacctgctg aaaaacatca acgctgactt tctgctcgaa
     5161 agcatacgca aagccgccga aggcgataat gtattctcgc ccgagatgac cgccaaactc
     5221 gttaaaagcc tgatttcccc ccaacctgcc caagggacac aggcactctc ctcacttacc
     5281 cctcgcgaac tggaaatctt gggctatctc gccgcagggc acagcaacaa aatcatcgcc
     5341 cgccacctcg atcttgccga atccaccgtc aaagtccacg ttcaaaacct gctccgcaaa
     5401 ctcaacctca gcagccgggt gcaggccgct gtttacgcca tccggcacaa cgtcccccaa
     5461 cccatgctgg aataaggcgg gttcagacgg catattaggg gttttaatcc ccgtacggtc
     5521 attcggataa cagaccaagc atgtaagttt atgcccccat aagtacgctt ggcatagcag
     5581 taatattgtt cggttcagtg ttttccgttt gcccctatct gatactgcaa tatcagctat
     5641 gccgtctgaa aacgcatcat catgatattt tcagacggca taataaaaag cggaaatact
     5701 aatgcagggt aaaatgttcc attccgaatc ccataaatat acaatggctt atatcgttta
     5761 agcatgtgta acccaccctc atatcaatca a
//
LOCUS       DAXHTR010000131         5745 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.131, whole genome shotgun sequence.
ACCESSION   DAXHTR010000131 DAXHTR010000000
VERSION     DAXHTR010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.131"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(62..883)
                     /locus_tag="ACFNML_001705"
     CDS             complement(62..883)
                     /locus_tag="ACFNML_001705"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HFC6393047.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASGQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            1295..3679
                     /gene="ppsA"
                     /locus_tag="ACFNML_001706"
     CDS             1295..3679
                     /gene="ppsA"
                     /locus_tag="ACFNML_001706"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFC6393048.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLENVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPMLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFAINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITRRIAGYASPVDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSVYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            3899..5158
                     /gene="rho"
                     /locus_tag="ACFNML_001707"
     CDS             3899..5158
                     /gene="rho"
                     /locus_tag="ACFNML_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFC6393049.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTANYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEATEFLNGKIKASKNNDDFFELMRGK"
     gene            5464..>5745
                     /locus_tag="ACFNML_001708"
     CDS             5464..>5745
                     /locus_tag="ACFNML_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393050.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKSTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFAERQNHINGIENFWNRAKRHLRKF"
BASE COUNT         1527 a         1647 c         1343 g         1228 t
ORIGIN      
        1 aactgccgtc tgaaacctga tttcagacgg catttccacc ttcaaaccga atcaaagccc
       61 gtcaaaacct gcgtttgagc ttgcacgcct gaaggatgtg tacggcaagc tcttccactg
      121 atttatccgt cgtattcgca aacggaatgc catgccgtct gaacatactc tgcgcgtccg
      181 ccacctcgct gcggcatgta tcgattttgg cataagttga attcgggcgg cgctcttggc
      241 ggatggcctg caaacgttcc ggctggatgg tcaacccgaa cagcttatcc ctataaggct
      301 tgaccatacg cggcagatcg gccgattcca aatcgtcggg aatcagcgga tagtttgccg
      361 cacggatgcc gtattgcagg gcgaggtaaa ggcaggtcgg cgttttgccc gaacgcgata
      421 cacccatcaa gattacatcc gcttcctgaa ggttcttatc gctgaccccg tcatcgtggt
      481 tcaatgagaa attcaccgcc tccatacgtg catcgtaacg cttcgtatta ccgatactgt
      541 gatgcccctg cccggatgcc gtggcttcgg tattgagttc tttctccaac agtcccaaaa
      601 aagtctcaaa gaaattaatc tgaaaagcat ccgccccttt gataatccga cggatttcgt
      661 catcaaccac actgacaaac gcaatcggac gctgaccgtt ttcctgccgg ctccgattga
      721 ccttctccac caccgcgcgc gccttttccg gcgtatcgac aaacggatgc gtatggcgtt
      781 tgaacgacag attgccaaac tggttcagca aagcctcgcc gatattctcg gcagtcagac
      841 cggtacggtc ggaaatgtaa aacacatggc gcggactgct catcttccat ccttaaaaca
      901 caggtttaaa atctcatcat aacagcagca gcagacacaa ggaaagcacc cgcagcacac
      961 ctacctcgga ttacacccaa acagacacaa tattattttg aaataaaatt atttagataa
     1021 agtgtttttt ggcataaaat tttaaaaaaa taaacaaaaa atcaaacaga aaaaacatta
     1081 acctattcaa accatctgtt ttacaaagaa aatacccaaa aaaagaaaca taccggctgc
     1141 aagtttcaaa ccgctacaca cgccgaaacc gcaatttttc agacggcatc atgattttaa
     1201 aacggataaa acacatgaca gcagaggaac gaatcgctta aaataagcac gcggatttgt
     1261 ttctttttta acatattttg gattggacac acaaatggcc gacaactacg taatctggtt
     1321 tgaaaacctg cgtatgacag atgttgaacg cgtgggcggt aaaaacgcct cgctgggcga
     1381 aatgatcagt cagctgaccg aaaaaggcgt tcgcgtcccc ggcggctttg ccaccacagc
     1441 cgatgcctac cgcgcattcc tcgcacacaa cggtctgagc gaacgcattt ccgccgcact
     1501 ggcaaaatta gatgtcgaag acgttgccga actggcacgc gtcggcaaag aaatccgcca
     1561 atggattttg gacacgccct tccccgaaca gctcgatgcc gaaatcgaag cggcatggaa
     1621 caaaatggtt gccgatgccg gcggtgcgga catttccgtt gccgtacgct cttccgcaac
     1681 cgccgaagac ctgcccgacg catcatttgc cggccaacag gaaaccttct tgaacatcaa
     1741 cggattggaa aacgttaaag aagcgatgca ccatgtattc gcctccctgt acaacgaccg
     1801 cgccatttcc taccgcgtcc acaaaggctt cgaacacgac atcgtcgccc tttccgccgg
     1861 cgttcaacgc atggtgcgtt ccgacagcgg cgcatcaggt gtgatgttca ccctcgacac
     1921 cgaatccggc tacgatcaag tcgtctttgt tacctcctct tacggtctgg gcgaaaacgt
     1981 cgtacaaggt gcggtcaacc cggacgaatt ttatgtgttc aaacctatgc tcaaagcggg
     2041 caagcccgcc atcctgcgca agaccatggg ttcgaaacac atcaaaatga tttttaccga
     2101 caaagcagaa gccggtaaat ccgtaaccaa cgtcgatgtc cccgaggaag accgcaaccg
     2161 cttctccatt accgacgaag aaatcactga gttggcgcat tacgcactga ccatcgaaaa
     2221 acactacggc cgcccgatgg acatcgaatg gggacgcgac ggcttggacg gcaaactcta
     2281 catcctgcaa gcccgtcccg aaaccgtcaa atcccaggaa gaaggcagcc gcaacctgcg
     2341 ccgcttcgcc atcaacggcg aaaaaaccgt attatgcgaa ggccgtgcca tcggtcagaa
     2401 agtcggacag ggcaaagtac gcctgattaa agatgcttcc gagatggatt ccgtcgaagc
     2461 cggcgacgta ctggttaccg acatgaccga tccggattgg gaacccgtga tgaaacgggc
     2521 ttccgccatc gttaccaacc gtggcggacg cacctgccac gcggcgatta tcgcgcgcga
     2581 actgggcatt cctgccgttg tcggctgcgg caatgcaacc gaattgctga aaaacggtca
     2641 agaagttacc gtatcctgtg ctgaaggcga taccggcttc atctatgccg gtctgttgga
     2701 cgtacaaatt accgatgtcg ccttggacaa tatgcccaaa gcacccgtca aagtcatgat
     2761 gaacgtcggc aaccccgaac tcgcattcag cttcgccaac ctgcccagcg aaggcatcgg
     2821 cttggcgcgt atggaattta tcatcaaccg ccaaatcggt atccacccga aagccttgtt
     2881 ggaatttgac aaacaagatg acgaattaaa agcggaaatt acccgccgta tcgccggtta
     2941 cgcgtcccct gtcgacttct acgtcgataa aatcgccgaa ggcgtggcga cattggccgc
     3001 atcggtttat ccgcgtaaaa ccatcgtccg tatgtccgac ttcaaatcca acgaatacgc
     3061 caacctggtc ggcggcagcg tatacgaacc gcatgaagaa aacccgatgt tgggcttccg
     3121 tggtgcggcg cgttatgtcg ccgacaactt caaagactgt ttcgccttgg aatgcaaagc
     3181 cttgaaacgc gtccgcgatg aaatggggtt gaccaacgtt gaaatcatga ttccgttcgt
     3241 ccgcactttg ggcgaagccg aagccgttgt caaagccctg aaagaaaacg gcttggaacg
     3301 cggcaaaaac ggcctgcgcc tgattatgat gtgcgagctg ccgagcaacg cggtattggc
     3361 ggaacaattc ctgcaatact tcgacggctt ctccatcggc tcgaacgaca tgacccaact
     3421 gaccctcggt ctcgaccgcg acagcggctt ggtatccgaa tcgtttgacg aacgcaaccc
     3481 tgccgtcaaa gtgatgctgc accttgccat ctccgcctgc cgcaagcaga acaaatatgt
     3541 cggcatctgc ggtcaaggcc cgtccgacca tccggacttc gccaaatggc tggttgagga
     3601 aggtatcgaa agcgtttccc tgaacccgga taccgtcatc gaaacttggc tatatttggc
     3661 gaatgaattg aacaaataat caatgcccat acccccgagc ctgaaaagcg cgggggtatt
     3721 tttttccaag cagctccgct cagacggcat ttcctgccga tgccccgtcc gcgataatat
     3781 ttgacaccca cgcgccgact gcctacaatt cccccttccc cgagcaaccg gcaacggtca
     3841 gcttcttctt tcagacggca tctgcctgtc tttttcctct tcaaaataca tcattattat
     3901 gcacgtctcc gaattacaaa ccctgcacat ttccaaactc ttagaattgg cggaagaaca
     3961 cggcatcgaa aacgccaacc gattccgcaa acaagacctc gtatttgcca tcgtccgcca
     4021 aatgatgaaa aaaggcgagg gtttcacctg ctccggcacg ctcgaaatcc tgcccgacgg
     4081 cttcggcttc ctccgcagcg cggacacgtc ctatcttgcg ggccccgacg acatctatgt
     4141 ctcgcccacc caaatccgcc gcttcaacct gcatacgggc gacaccatcg aaggcagcgt
     4201 gcgcgtcccc aaagacaacg aacgctattt tgccctagtc agactggatt ccatcaacgg
     4261 cgaccacccg gaagtatgcc gccataaaat cctgtttgaa aacctgaccc cgctgtttcc
     4321 gaccgaacag ttgaagctgg aacgcgactt aaagtccgaa gaaaacctga ccggacgagc
     4381 catcgatctg atttccccca tcggcaaagg tcagcgcgcc ctcttggttg ccccgcccaa
     4441 aagcggtaaa accgtgatgt tgcaaaatat tgcccacgcc gttaccgcaa actatcctga
     4501 agtcgagctc atcgtcctct tgattgacga gcgtcccgaa gaagtaaccg aaatgagccg
     4561 ttccgtccgc ggcgaagtgg tttcctccac ctttgacgaa ccggcgcaac gccacgtcca
     4621 agttgccgaa atggtgcttg aaaaagccaa gcgtatggta gaacacaaaa aagacgtggt
     4681 catcctgctg gattcgatta cccgccttgc ccgcgcctac aataccgtcg tccccacctc
     4741 gggcaaaatc ctgaccggcg gtgtcgatgc caacgcgctg caccgcccca aacgtttctt
     4801 cggcgcagcg cgcaacgtgg aagaaggcgg ttcgctgacc atcattgcca ccgcattggt
     4861 tgaaaccggc agccgtatgg acgatgtgat ttacgaagaa ttcaaaggca ccggcaatat
     4921 ggaattgcac ctcgaccgcc gtatggcgga aaaacgcctc ttccccgcca tcaacatcaa
     4981 caaatccggc acgcgccgcg aagagctgct tgtgccgaac gaccagttac aacgtatgtg
     5041 gctcttacgc aagttcctgc acccgatgga tgaaatcgaa gctaccgagt tcttaaacgg
     5101 gaaaatcaaa gcctctaaaa acaacgacga tttctttgaa ctgatgcgcg gcaaataaac
     5161 gcgccgcccg cattaatgcg aaatgccgtc tgaagcctga aaatcgggtt tcagacggca
     5221 ctttcattca cacggtcggc gcagctcctc cctccccccg ttaacggcgc aaccgtcggc
     5281 agtgtccgtg tccgcttgcc gaaagcgcgg cctttgcaaa accggcttga acgcatccgt
     5341 accggcaatg aaaaccgatg cgggaacttg attcaaggtt gcgccgaacc gcgctcattt
     5401 ttgtataaat ttggggctgt cctagataac tagggaaatt caaattaagt tagagttgcc
     5461 cctatgagaa aaagtcgtct aagccggtat aaacaaaata aactcattga actgtttgtc
     5521 gcaggtgtaa ctgcaagaac agcagcagag ttagtaggcg ttaataaaag taccgcagcc
     5581 tattattttc atcgtttacg attacttatt tatcaaaaca gtccacattt ggaaatgttt
     5641 gatggcgaag tagaagcaga tgaaagttat tttgctgaac gacaaaacca tattaatgga
     5701 attgagaact tttggaatcg ggcaaaacgt catttacgca agttt
//
LOCUS       DAXHTR010000132         5736 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.132, whole genome shotgun sequence.
ACCESSION   DAXHTR010000132 DAXHTR010000000
VERSION     DAXHTR010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5736
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.132"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(968..3244)
                     /gene="znuD"
                     /locus_tag="ACFNML_001709"
                     /gene_synonym="tdfJ"
     CDS             complement(968..3244)
                     /gene="znuD"
                     /locus_tag="ACFNML_001709"
                     /gene_synonym="tdfJ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118820519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc receptor ZnuD"
                     /protein_id="HFC6393051.1"
                     /translation="MAQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     NKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSRADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFKNKTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLIDNNVRHYSFFGVEQANWDNFTLEGGVRV
                     EKQKASIRYDKALIDRENYYNHPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGKRPFIAQADQNAPRVPAARLGVHLKASLTDLIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            3666..4256
                     /locus_tag="ACFNML_001710"
     CDS             3666..4256
                     /locus_tag="ACFNML_001710"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HFC6393052.1"
                     /translation="MLLFIDNYDSFTYNIVQYFAELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIDRDNLPDCLEITAWTDDGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLIEFQNFKPQKI"
     gene            4319..5377
                     /gene="trpD"
                     /locus_tag="ACFNML_001711"
     CDS             4319..5377
                     /gene="trpD"
                     /locus_tag="ACFNML_001711"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HFC6393053.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQIMRGQVLPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVVEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(5434..5736)
                     /locus_tag="ACFNML_001712"
                     /pseudo
     CDS             complement(5434..5736)
                     /locus_tag="ACFNML_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
BASE COUNT         1394 a         1328 c         1560 g         1454 t
ORIGIN      
        1 gaaactgaac gaattagatt cccgcctgcg cgggaatgac aactcattag ttacctaaca
       61 cttaaaaaac agaaaccttt ccgcgtcatt cccacgaaag tgggaatccg ggaacttaac
      121 gttacagtga tttatcggaa acgactgaaa ccgaagagac cggattcccg cctgcgtggg
      181 aatgacaact cattagttac ctaaaactta aaaaacagaa acctttacgc cgtcattccc
      241 acgaaagtgg gaatccggga acttaacgtt acgacgattt atcggaaacg actgaaaccg
      301 aagagaccgg attcccgcct gcgtgggaac gacaactcat tagttactta aaacttaaaa
      361 aacagaaacc tttacgccgt cattcccacg aaagtgggaa tctagaaccc aaatgctaag
      421 gcgatttatc ggaaacggct gaaaccgaac gaattggatt cccgtctgcg cgggaatgac
      481 gactcattag ttacctaaaa cttaaaaaac agaaaccttt acgccgtcat tcccacgaaa
      541 gtgggaatcc gggaacttaa cgttacagtg atttatcgga aacggctgaa accgaacgaa
      601 ttggattccc gcctgcgcgg gaatgacgac ttattagtta cctaaaactt aaaaaacaga
      661 aacctttacg ccgtcattcc cacgaaagtg ggaatctaga acccaaatgc taaggcgatt
      721 tatcggaaac ggctgaaacc gaatgaattg gattctcgcc tgcgcgggaa tgacgggatc
      781 ttgggtttct gcttttgatt tttctgcttt tgcgagaatg acggcgtgaa agcaagaatg
      841 atgaaacaaa gaaaatggga atgatggcat agtggtttgt tctttgtctt tgccatattt
      901 cctaaaaaat tgattaaaaa aggttttcag aatgccgtct gaaaaccttt tttctgcctg
      961 tccgatttta aaacttcacg ttcacgccgc cggtaaagct gcggcccatt tgcggcgtat
     1021 cagagagaaa gctgctgtgg gcgtaaacgg attggttgag caggttgtcg gctttgacgt
     1081 accaattcca ctcgccatag cgcgtattgc ggcggtagtt tgcgccgagg ttgagcatat
     1141 ggtgtccggg cgtacgcgtt tcgtagcggg cgagtttgtt ttgggcgaac acgcggtagt
     1201 agtccaagtt ggcatcgatg aggtcggtca gcgaggcttt caggtgaacg ccgaggcgcg
     1261 cagccggaac gcgcggcgcg ttttggtcgg cttgtgcgat gaagggacgc ttgccgtaag
     1321 catcttccct gccgggtagg gaaggcaggt ttttcagacg gcctcgtaca tagtcgccgg
     1381 aaacaccgat gcggtagcgc ggtgtcggtt tgaagtagat ttcaccttcc gcgccgtaga
     1441 agtctgcgcc ggattggttg tagcgcacga gcttcatttc gctgtcgtct tcgatggatt
     1501 tggggccgcg tccgtcgttt aaggtttggg cgtaaatgta gttgccgaag cggttgcggt
     1561 agagtgccag attgtattgc cagcggtcgc cttcgtagcc caacgcgagt tcgatgttgt
     1621 tggaacgctc tttgttcagg tgtttgttgc cgacttcaaa agtgttggtg gcaacgtgtt
     1681 tgccgtgtgc gtacagctct tgcgttgacg gcaggcgttc ctgatgggag gcggtcaggc
     1741 tgagtttgtg ttgcggcgtg aaataccagt tgcccgaaag tgcgaacgag cgggcggttt
     1801 ggcggtgcgc gccgaggtcg ggcagggggt ggttgtagta gttttcccga tcaatcaatg
     1861 ctttgtcgta ccggatggag gctttttgtt tttccacgcg tacgccgcct tcaagcgtga
     1921 agttgtccca gtttgcctgt tctacaccga aaaagctgta atggcgcaca ttgttgtcaa
     1981 tcaacatcgg ttgtttgacc gcttcggatg tggcagataa agcactggat ttttgttgta
     2041 aatattgcac gccccagctg cctttcagac ggcctatggg ttggtggcgc aactcgatgc
     2101 gggcgttttg cgttttgttt ttgaagaagt tttccactgc atcgcctgct ttttcgtcgt
     2161 ggcggtagtc gttgcggttc aagtatacgc gcagggcttc aaaaccgggg aacggttgct
     2221 tccattcggc acggagttcg tagcgtttgt tgcgcaggtc tatccacggt ctgccgctgt
     2281 gggtgtgtgc gtgtgcatta tcgtcgtcgt ggaagccgca gctcaagccc ggattgtcgt
     2341 aatcgatgtc ttcttcggtc aacaggtgcg gataaagctg taaatagcgt ttgttaatca
     2401 agctcttttg ccagatgatg tcggcgtggc aatcatcgta ttcgtggctg tgggcaggca
     2461 gaccatattg gtcgcgacgg tcgctgtacg ctacgccgat aaaacctttt tcgccaaccc
     2521 aagacagccc gatgctgccc gtttgcgaat cggcgcggct gtcgggcagg cgtttcagat
     2581 tgcggtaacg cggtacggcg taatcgcccg atttgcggta caagccttcc gtatgcaata
     2641 caaagttttt gcccaagccg atattgatgc ctgcggatgt cagtttttcc aaattgccgc
     2701 tgctcaaacg caatccggct tcgcccgata cgccgttttc aggcattttt tcgggaattt
     2761 tattgtcggc aacatcaacc agccccgcga cattgcccga actatataaa agcgtaaccg
     2821 ggccgcgcag gatttcgacc tgttgcgaca aggcggtatc taccataatg gcgtgatcgg
     2881 gcgaaaaatc cgccatatcg cccgtttcgc cgtgatggtt caaaacctta atccgcctgc
     2941 ctgtttgacc gcgtatcacc ggtgcggacg cgccgccgcc gtattgcgag gcgtgaatac
     3001 ccggtacgcc gtccaaagca tcgcctaagt tgacggcttt ttggcgcaag gtatcgccgg
     3061 agatgatttt gtcggaggcg gtcgaagtgt gcagcagccc cgaagtggcg cgcggacggc
     3121 ttttgccgac gacgctgacc gtttccaaat ccaccgattg ctcagtttca tgcgcttggg
     3181 cgaggagggg tgtgttgatt aaaagaattg ataaaacaat gggtttgagt gtagtttgtg
     3241 ccattttggt ttctcgtcgc atttcaaaag tttgttatta tataacatta cattccttat
     3301 atcataagat tttgagaaca ctcagagggc ataggcaaaa gtttttcaaa tgaaacggtt
     3361 gcggcatcgg gcggtgtcca tttgtatccg ccgtccttcg ggggcgcggt tgatgttgac
     3421 gcagattccg ctgtgtttgc ccattatgtt cggctgcggg ataaccaaaa tttatgagtg
     3481 cataaaaacg gtacgttccc gaacggtcga aaaggtggca aaatggcgta cttgtcagaa
     3541 cgcgaggctc tgcgccaggt tcacgagggt gcatcgggca cgctaagata taagagggta
     3601 tggatggggg tatcggaaat gcagttaata acaaacaaat tataaatcaa taggttaata
     3661 acaaaatgct tttgtttatc gacaattacg acagttttac ttacaacatc gtccagtatt
     3721 tcgctgaatt ggggcaggaa gtcgccgtgc gccgcaacga tgatattacg ttggaagaaa
     3781 tcgaggcatt gaatccgcaa tatctcgtta tcggacccgg tccgtgttcg cccaaagaag
     3841 cggggatttc cgtggcggcg atgcgccatt ttgccggccg gctgccgatt atgggcgtgt
     3901 gcctcgggca tcagacgata ggcgaggcgt tcggcggcag gatagtccgc gccaaaacgc
     3961 tgatgcacgg taaggtgtcg cccgtgtccc attcgggcaa gggtatgttt aaaggtttgc
     4021 ccaatccggt tacctgtacg cgttatcaca gcctcgttat cgatcgggat aatttgcccg
     4081 attgtttgga aatcacggct tggactgacg acggcgagat tatgggcgtg cgccataagg
     4141 aatatgccgt cgagggcgtg cagttccacc ccgaagccct cttgaccgaa cacggacatg
     4201 atatgttgaa caatttttta atcgaatttc aaaacttcaa accgcaaaaa atctgacgcg
     4261 atgccgtctg aaggcttcag acggcatttt cgtccgaata ttgaacggag gacaaaaaat
     4321 gattacaccg caacaggcta tcgaacgatt aatcagcaat aacgagttgt tttacgatga
     4381 aatgaccgac ttgatgcgtc agattatgag aggacaggtt ctgccggagc agatagcggc
     4441 cattttgaca ggattgcgta tcaaggttga aaccgtttcc gaaattaccg cagctgcagc
     4501 cgtcatgcgc gagtttgcga caaaagtgcc gctggagaat gcagaggggc tggtcgatat
     4561 cgtcggtacg ggcggggatg gtgccaaaac cttcaatatt tcgacgacct cgatgtttgt
     4621 tgccgcagcg gcaggcgcga aggttgccaa acacggcggg cgttccgttt catcttccag
     4681 cggcgcggcg gacgtggtgg agcagatggg cgcaaacctc aacctgactc ccgaacagat
     4741 tgctcaaagt atcagccaga ccggtatagg gtttatgttc gcccccaatc accacagtgc
     4801 catgcgccat gtcgccccgg tacgccgttc gctcggtttc cgaagcattt tcaacatatt
     4861 gggtccgttg accaatcctg cgggcgcgcc gaaccaactt ttgggcgtgt tccacaccga
     4921 tttgtgcggc attttgtcgc gggtcttgca acaacttggt tcaaaacacg ttttggtcgt
     4981 ttgcggggag ggcggcttgg atgaaattac actgacgggc aaaacgcgcg ttgccgaact
     5041 caaagacgga aaaatcagcg aatacgacat ccgcccagaa gatttcggta tcgaaacccg
     5101 ccgcaatttg gatgaaatca aagttgccaa tactcaggaa tctttgttga aaatgaatga
     5161 ggtgctggat gggaaagaag gggctgcgcg cgatatcgta ttgcttaata ccgccgccgc
     5221 cctatatgcc ggaaatgtcg ccgcttcgct ttcagacggc atatctgccg cacgggaagc
     5281 catcgattca ggtagggcaa aatcgaaaaa agaggagttt gtcggttttc aaccacaaca
     5341 aagatgccat tttcttggaa agatggagct tgggtgatgc cgccatgatt atggaacttt
     5401 tgtggcaaaa cataagcact tcacgaagag aacttaccaa actgttttta tataaaaact
     5461 tggggctgta ctagataacc agaccaaatt cccattaact aattgtctta aaatctgaat
     5521 ttgagattct atttaaaatg ccattggcat ttctttaaat gcagccccaa atgctctttg
     5581 ggaatgccgt taaacttacg taaatggcta aattcactaa tatcaagcac atcataacta
     5641 cgaaaggtat ccgtgtatac aatgccatca ggcttaactt tctttcggat aattggcaac
     5701 aatgtcgctg attgcgcatt aggaacaacg acggta
//
LOCUS       DAXHTR010000133         5657 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.133, whole genome shotgun sequence.
ACCESSION   DAXHTR010000133 DAXHTR010000000
VERSION     DAXHTR010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.133"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            97..813
                     /locus_tag="ACFNML_001713"
     CDS             97..813
                     /locus_tag="ACFNML_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFC6393054.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILADLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            962..1864
                     /locus_tag="ACFNML_001714"
     CDS             962..1864
                     /locus_tag="ACFNML_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HFC6393055.1"
                     /translation="MDSLMTLLSVLIPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGTNLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSENAGMYCLMLLVLLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASADGVSWVKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIALLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIRGAGGLEAVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1907..2680
                     /locus_tag="ACFNML_001715"
     CDS             1907..2680
                     /locus_tag="ACFNML_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFC6393056.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EDAGSLKRHHGGASSGGNLPEGLPASRQIQCQNEKNAIARLIAEHIPDESSLFVSIGT
                     TVEAVASELVRRRGSLRIITNNIHVASIVSARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2792..4480
                     /locus_tag="ACFNML_001716"
     CDS             2792..4480
                     /locus_tag="ACFNML_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HFC6393057.1"
                     /translation="MLNKDQFADNHFIRTFIEEDLKSGKHETIQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLVKDGKAYVDDLTPEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIPAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFETGRTQSRRAAFHPNHEEMGDREVPVSQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVLELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLPFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4573..5529
                     /gene="gshB"
                     /locus_tag="ACFNML_001717"
     CDS             4573..5529
                     /gene="gshB"
                     /locus_tag="ACFNML_001717"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFC6393058.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMQLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELSERDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
BASE COUNT         1311 a         1546 c         1560 g         1240 t
ORIGIN      
        1 atttgtgtaa tactttgatt tccccgacac gatttgaagc acagccgcct taaaacatct
       61 taaaaaattt gaaagcgtaa aaatacggaa aacatcatga actttgaaaa cgacgacatt
      121 atccatgcgc cgaccacgtc ttccttgatt ctcgaagagc ggcacgattc ggagctgttc
      181 cgtgtttacg ccctgatttt ggacggcatt accgatcagg tgctgctgcc cggcaaaaag
      241 ctgaccgaat ccgaactttg ccgccagatg gtgtgttcgc gcaacaccgt ccgcggcgcg
      301 ttgtcgcttt tggcgcacga caagattgtc gatttgcaac ccaacagggg cgcgttcgtc
      361 cacgttcccg atttgaaaga aatgcaggat gtgttcaatg cgcgcatcga aatggagacg
      421 atgattttga atatcctcgc agatttgccg gatttggaaa cgcgcctcaa gccgctttat
      481 gcgatgatac ggcgcgaaga agaggcctcc ggcaggggag accgcgttgg ctggaaccgc
      541 ctgtccaatg ccttccacgt cgaactggcg cgcctggtgg gcaacgatgt gctgttcgac
      601 attatgaaca cgctgtgcgc gcggtcttcc ctgattgtcg ccgtggcggg cgtgcatcgc
      661 gaggaaaaac acgccatcaa tacgcatacg cattccgaac accgcgaaat cctcgacctg
      721 ctgctggcgg gcaggcgcaa ccgcgtggtc aaaatcctgc gccgccattt gggcaactgt
      781 atggagcgtt tggaaaagac tttggaagat tgaatgccgg agcggataag ccgcctgaac
      841 cttcaggcgg ttttttaatg gcgaacctga tgccgtctga aatatggatg cgggtatctg
      901 caattttcag acggcaattt ttaaaccgca tatatcatgc ggcaataaag gaggtcagga
      961 gatggacagc ctgatgacgt tgctttcggt attgataccg atgtttgccg gattttttat
     1021 ccgtgtgccc aagccttacc tgcccgcttt ggataaggtg ctatcggtct tggtgtatgc
     1081 tgtgctgctg ctgatcggcg tctcgttgtc gcgcgtggag gatttgggtt cgcggttgga
     1141 cgatatggcg ttgacggttc tgtggctgtt tgtttgtacg gtcgggacga acctgcttgc
     1201 tttggcagtg ttgggaaagt tattcccgtg gcggataaag gggaaaggga agggcgtttc
     1261 ggtcggtgtg tcgggcagtg tggggcagct cggatgcgtg ctgctcggat ttgcatccgg
     1321 caaactgatg cgcgatattt ggatgccgtc tgaaaacgcg ggtatgtatt gtctgatgct
     1381 gctggtgctc ctcatcggcg tacagctcaa aagcagcggc gtatcgttgc ggcaggtttt
     1441 ggtcaaccgc aggggtattc ggttgtcggt ctggtttatg ctttcatctc tttcaggcgg
     1501 gctgctgttt gccgcatcgg cagacggtgt gtcgtgggtg aaaggtttgg cgatggcttc
     1561 cggcttcggt tggtattccc tctcgggttt ggtgatgacc gaggcttacg gcgcggtatg
     1621 gggcagtatc gcgcttttga acgatttggc acgagagctg ttcgcgctgg catttattcc
     1681 gctgctgatg aagcgttttc ccgatgcggc agtgggggtc ggcggcgcga ccagtatgga
     1741 tttcacattg cccgtgattc ggggtgcggg cggcttggaa gccgtaccgg tagcggtcag
     1801 cttcggcgtg gtggtcaata tcgccgctcc gtttctgatg gtggtgtttt ccgctttggg
     1861 ctgaacgcgg taaaatcggc atcccgatgc aaggaggcag aaaacgatga aaccgaaaat
     1921 ccaaaggcac ggagagattt taagccttgt ccgccggcat cagtttatgt cggtggacga
     1981 gcttgccgcc gcattggacg ttaccccgca gacgatacgc cgcgacatcc gcgaattgga
     2041 ggatgctggc agcctgaaac gccaccacgg cggcgcatcc tcgggcggaa acttgccgga
     2101 ggggctgcct gccagccgcc aaattcaatg tcaaaacgaa aaaaacgcca ttgcccggct
     2161 gattgcggaa catattcccg acgagtcatc gctgtttgtc agtatcggca cgacggtgga
     2221 agccgtggca tcggagctgg tgagacggcg cggcagcctg cggattatta ccaacaatat
     2281 ccacgtcgcc tccatcgttt cggcgcggac ggattacacg gtcatcatca cttccggagt
     2341 cgtccgccct ttggacggcg gcattaccgg cgtggcaacc gtcgatttta tcaaccagtt
     2401 caaagtcgat tatgccgtga tgagtacgca cggcgtggaa agcgacggtt cgcttttgga
     2461 cgacgactac aaagaagtca gcgtgatgca ggcgatgatt gccaacgcgc gcgtccgctt
     2521 cctcggcgtg gaccacagta aattccgcag caacgcgctg gtcaggctcg gcgacattac
     2581 ggcgtttgac aaagtattta ccgaccggct gcccgatacc gcgatgcaga agatgctgaa
     2641 agaggcgggg gtggaatgcc tgattgccga tgccgtctga acgctatgtc aaagcgcgca
     2701 agtcgggtac aataaacaca tcatcaaacc gcttcagacg gcataaggaa ccgccccaat
     2761 gccgtctgaa gccatctgtt caaagaaaac tatgctcaat aaagaccaat ttgcggacaa
     2821 ccattttatc cgcaccttca tcgaagaaga cctcaaaagc ggcaaacacg aaaccatcca
     2881 aacccgtttt ccgccggagc ccaacggcta cctgcacatc ggacacgcca aatccatctg
     2941 cctgaacttc ggtttggcgt atatttacga cggcttgtgc aacctgcgtt ttgacgatac
     3001 caaccccgaa aaagaaaacg acgaatacgt caacgccatc aaagaagacg ttgagtggct
     3061 cggtttccat tgggcgggcg agccgcgttt cgcttccaac tatttcgacc agctttatga
     3121 ctacgccgtc ggtttggtta aagacggcaa agcgtatgtc gatgatttga cgccagaaga
     3181 aatgcgcgaa taccgcggta cgctgaccga agcaggcaaa aacagccctt atcgcgaccg
     3241 cagtatcgaa gaaaacctcg acctgttcac gcgcatgaaa aacggtgagt tccccgacgg
     3301 cagcaaaacc ctgcgcctga aaatcgacat ggcatccggc aacatcaata tgcgcgatcc
     3361 ggtcatctac cgcatccgcc gcgcccatca ccacaacacc ggcgacaaat ggtgcatcta
     3421 tccgatgtac gactacacgc attgtatttc cgatgccatc gaaggcatca cgcattcctt
     3481 gtgtacgctt gagtttgaag cccatcgtcc gctgtatgac tgggtgttgg acaacatccc
     3541 cgcgccgcac gccacacgtc cgcgccaata cgagttttca cgcttggagc ttttgtacac
     3601 cattacctcc aaacggaaat tgaatcagtt ggttgtggaa aaacacgttt ccggctggga
     3661 cgatccgcgt atgccgacca tttccggtat gcgccgacgc ggctacacgc ccgaagggct
     3721 gcgcctgttt gccaaacgcg ccggtatttc caaatctgaa aacatcgtcg atatgagcgt
     3781 gttggaaggc gcgattcgcg aagagttgga aaattccgcc ccacgcctga tggcggtgtt
     3841 gaacccgctc aaagtaaccc tgaccaactt tgaaaccggc agaacccaaa gccgccgtgc
     3901 cgcgttccat ccgaaccacg aagaaatggg cgatcgcgaa gtacctgttt cacaaaccat
     3961 ttacatcgaa gccgacgact ttgccgaaaa tccgcccaaa ggcttcaaac gcctgattcc
     4021 cggcggcgaa gtacgcttgc gccacggcta tgtcatcaag tgcgatgaag tcgtcaaaga
     4081 cgaagcaggc aatgtgcttg aactcaaatg cagcatcgac cacgacacct tgggcaaaaa
     4141 tccagaaggc cgtaaagtta aaggcgtgat tcattgggtt tccgccgaac acgccgccga
     4201 aatcaaagtc cgtctgtacg accgcctctt taccgtcgag cgtcccgatg ccgtgcgcgg
     4261 cgaagacggc gaatacctgc cgttcaccga tttcctcaat ccggaatccg tcaaggaaat
     4321 caccgcctac gccgaacctg ccgccaaaga tttgccggca gaaagccgtt ggcagttcga
     4381 gcgtatcggc tacttcgtaa ccgaccgtaa agaccacggc aaagacacgc cggtgtttaa
     4441 ccgcacggtc acgctgaaag attcttggca gcctaagtaa aaccccatcc ttgccgtctg
     4501 aatattgttc ggacggcatt tcccctttgc ccgcgaaatg cggcacattc ggcacaccgg
     4561 aaaggaaata tgatgaaagt cctctttatc gccgacccga tggcaagttt caaaacctac
     4621 aaagacacca cctacgccat gatgcgcgag atggcaaaac gcggctggcg gctgtttcat
     4681 accttgagca gcggattgtc ggttaaaaac ggtctggtgg ttgctgatgc gtctgctttt
     4741 gagtttctcg gcgcacaaga cgaccacgac aaaaactggt ttgccgccgc cgacaaagtt
     4801 cagacggcat tgaaagcatt tgatgccgtg attatgcgta ccgatccgcc gttcgatatg
     4861 caatacctct acgccacaca gcttctgacg ctggcggagc agcagggcgc gaaggtcttt
     4921 aacagcggac agtcgatgcg tgacttcaat gaaaaactgg cgattttgaa cttcagccgc
     4981 tttaccgcgc ccacgctggt aacgacccgt tccgccgatg tccgcgcatt tttgaaagaa
     5041 cacggcgaca tcatcattaa accgctcgac ggcatgggcg gtatgggcat cttccgcctg
     5101 accgaaaaag accccaacat cggcagcatc ctcgaaaccc tgatgcagct tgattcccgc
     5161 accattatgg cgcaacgcta cattcccgaa atcgtacacg gtgacaaacg catcctgatt
     5221 atcggcggcg aagtcgtccc ctatgctttg gcgcgtattc ctcaaaacgg cgaaacacgc
     5281 ggcaaccttg cggcaggcgg gcgcggcgtg gcgcaggaat tgagcgaacg cgaccgcgaa
     5341 attgccgaaa cgctcgcccc cgaactcaaa cggcgcggca tcctgctggc cggtttggac
     5401 gttatcggca gcaacctgac cgaagtcaac gtaaccagcc cgaccggatt ccaagaaatt
     5461 atgaaacaaa aaggcttcga tgtggcggca atgtttgccg atgccgttgc cgcgtggtcg
     5521 gtacgttaaa ccgatgccgt ctgaaaggct tttgcttcac aaccgcctgg tttgatcggg
     5581 caggcagggt tttccctcag tcggcagaga tacgcttaat accgtacgga caccccgccg
     5641 ccccgttttc agacggc
//
LOCUS       DAXHTR010000134         5598 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.134, whole genome shotgun sequence.
ACCESSION   DAXHTR010000134 DAXHTR010000000
VERSION     DAXHTR010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.134"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            70..543
                     /locus_tag="ACFNML_001718"
     CDS             70..543
                     /locus_tag="ACFNML_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HFC6393059.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVEGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMPAMNHGHHHGEAHQH"
     gene            669..1772
                     /gene="mnmA"
                     /locus_tag="ACFNML_001719"
     CDS             669..1772
                     /gene="mnmA"
                     /locus_tag="ACFNML_001719"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFC6393060.1"
                     /translation="MNTTANTTNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGAGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQSTDKPVIITR"
     gene            1843..3513
                     /locus_tag="ACFNML_001720"
     CDS             1843..3513
                     /locus_tag="ACFNML_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HFC6393061.1"
                     /translation="MEKIWLDSYEQGVNAEIDITQYGSVSDVFCQSVKKFAGQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPIALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFVIRKIKKMVPEYRIRETISFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIVLITNPRDMKGFIGELKKQRVNVFIGVNTLFNAMVNRPDFAEVDFSGLRLTLGGG
                     MATQKAVAEKWKKITDTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVRGPQVMKGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKNIEFRDELPKSNVGKILRRELRQSTGK"
     gene            3625..5259
                     /locus_tag="ACFNML_001721"
     CDS             3625..5259
                     /locus_tag="ACFNML_001721"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HFC6393062.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDRTMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAIQNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGVHTETDVQITFVDSESIEK
                     NNGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     TGSLAAKIYGSGHIRERHRHRYEVNNNYVPTLEQAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
BASE COUNT         1482 a         1523 c         1409 g         1184 t
ORIGIN      
        1 gtactggttt ttgttaatcc gctataagat taagcacatt ccccgtctga tattataagg
       61 agcagaaaga tgaaaaaatt attggcggcc gtgatgatgg caggtttggc aggcgcggtt
      121 tccgccgccg gagtccacgt tgaggacggc tgggcgcgca ccaccgtcga aggtatgaaa
      181 ataggcggcg cgttcatgaa aatccacaac gacgaagcca aacaagactt tttgctcggc
      241 ggaagcagcc ccgttgccga ccgcgtcgaa gtgcataccc acatcaacga caacggcgtg
      301 atgcggatgc gcgaagtcga aggcggcgtg cctttggagg cgaaatccgt taccgaactc
      361 aagcccggca gctatcacgt gatgtttatg ggtttgaaaa aacaattaaa agagggcgac
      421 aagattcccg ttaccctgaa atttaaaaac gccaaagcgc aaaccgtcca actggaagtc
      481 aaaatcgcgc cgatgccggc aatgaaccac ggtcatcacc acggcgaagc gcatcagcac
      541 taatctgctg aaaatgtttg aaatgccgtc tgaaaaagcc cgggctttca gacggcattt
      601 ttatgaccgc ctttaaaatg tgttaaaatc tgcctttaaa aaccgccgtt tccaaccata
      661 ttctacgcat gaatacgaca gcaaacacca ccaatatcat cgtcgggctc tccggcggtg
      721 tcgattcttc cgtaaccgcc gccctgctca agcagcaggg ttatcaagtg cgcggtgtgt
      781 tcatgcagaa ctgggaagac gacgacaacg acgaatattg cagcatcaaa caggattcgt
      841 tcgatgccat cgccgttgcc gatattatcg gcatcgatat cgacatcgtt aatttcgccg
      901 cgcaatataa agacaaagtc tttgcttatt ttcttcagga atacagtgcg gggcgcacgc
      961 cgaatccgga tgtgttgtgc aacgccgaaa tcaaattcaa atgctttttg gactacgccg
     1021 tagggcaggg cgcggatacc atcgccaccg gccactatgc gcgcaaagaa gttcgcaacg
     1081 gcgtgcatta cctgctcaaa ggtttggatc gaaacaaaga ccaaagctat ttcctctacc
     1141 gcctcaagcc tttccaactc gaacgcgcga tttttccgtt gggcggtttg gaaaaacccg
     1201 aagtgcgccg ccttgccgcc gagttcaaac tgccgaccgc cgccaaaaaa gacagcacgg
     1261 gcatctgctt catcggtgaa cgcccgtttc gcgagtttct gcaaaaatac ctgccgaccg
     1321 acaacggtaa aatggttacg cccgaaggga aaaccgtcgg cgaacacgtc gggctgatgt
     1381 tctacacgct cggacagcgc aaaggtttgg gcatcggcgg cgcgggcgaa ccgtggtttg
     1441 ttgcggctaa agatttgacg aaaaacgaac tgattgtcgt gcaaggacac gaccatccgc
     1501 tgctctatac ccgcagcctt gtgatgaacg atttgagttt cacgcttccc gaacgcccga
     1561 aggcaggacg ctatacctgc aaaacgcgtt accgtatggc ggacgcgcct tgtgagttgc
     1621 gctatttgga tgaagaaacc gccgagctgg tgtttgacga accgcaatgg gcggttacgc
     1681 cgggccagtc tgccgtgctg tacgacggcg acatctgttt gggcggcggc attatccaat
     1741 caaccgacaa acccgtcatc atcacacgat aaaggtaatg ccgtctgaaa cggttttcag
     1801 acggcattgt tccgctcaat tccactttaa agaccgatac ctatggaaaa aatctggtta
     1861 gacagttacg aacagggcgt gaatgccgaa atcgacatca cgcaatacgg ttccgtcagc
     1921 gatgtatttt gccaaagtgt caagaaattt gccggccaac ctgcttttca aaacatgggc
     1981 aagacgctga cttatgccga aaccggcaaa ctggcaacag attttgcttc ttatctgcaa
     2041 aacgtcctga agctgccgcg cggcgaacgt gttgccatta tgctgcccaa cgtattgcaa
     2101 tacccgattg cccttttcgg cattttgcag gcaggtttgg tagcggtgaa caccaatccg
     2161 ctctataccc cgcgcgagct ggagcatcag ttgaaagaca gcggcgcgac cgccatcatc
     2221 gttttggaaa atttcgccaa cacgctggag ctggtgctgc cgcgcacgca gatcaaacac
     2281 gtcatcgtag cctccgtcgg cgaaatgttc gggctgctta aaggttcgct gatcaatttc
     2341 gtcatccgga aaatcaagaa aatggtgccc gaataccgta ttcgggaaac catttccttt
     2401 cagacggcat tgaaagaggg ggcgaagcac gttttccaac ctgtcgcatt aaaccgcgaa
     2461 gataccgcgc tgttgcaata cacgggcggc acgacaggtg ttgccaaagg ggcggtgctg
     2521 agccacggca acatctgcgc caatatgctt caggcaaaag aatggattaa aaaccagttg
     2581 cgtgagggaa aagaaaccgt tatcgccgcc ttgccgctgt accatatttt tgccttaacc
     2641 gtgaatctga tgatttttgc caatgccggc tcgaaaattg tcctgattac caacccgcgc
     2701 gatatgaaag gctttatcgg agaactgaaa aaacagcggg ttaatgtatt tatcggcgtg
     2761 aacacgctgt ttaacgcgat ggttaaccgt cctgattttg ccgaagtcga tttttcagga
     2821 ttgcggctga ctttgggcgg cggtatggcg acccaaaaag ccgttgccga aaaatggaaa
     2881 aaaatcaccg acacgcccat cgtcgaagcc tacggtctga ccgaagccag ccccggcgtg
     2941 tgctgcaacc ccttaaacat cgaatcatac agcggcagca tcggtttgcc cgtctcgtcc
     3001 accgaagtcg aactgcgcga cgcaaacggc aaagaagtcc ccgtcgggca gccgggcgaa
     3061 ttgtgggtgc gcggcccgca agtcatgaaa ggctattgga accgtcccga agaaaccgct
     3121 aaagccatag acgcgcgcgg tttcttggaa accggcgata ttgccgtcat ggacgaaaaa
     3181 ggctggttga agctggttga ccgtaagaaa gacctcattg ttgtttctgg ttttaatgtt
     3241 tatccgaacg aaatcgagga agtcatcgcg catcacggca aagtgatgga agtcgcctgt
     3301 atcggcgttc ccgacgaaaa aaccggagaa gctctcaaag tgtttgtcgt cagaaaagat
     3361 ccgtctttga cgaaagaaga acttatcgcc ttttgccgca ccgaattaac cgcatataaa
     3421 gtaccgaaaa atatcgaatt ccgcgacgag ttgcccaagt ccaacgtcgg caaaatcctg
     3481 cgccgcgagt tgcggcaaag tactgggaaa taaagaaaag atgccgtctg aaaacagccg
     3541 tccaccgttc agacggcatc cgtccgtttg ccagaaccgc gcgcttcaag ttaaaatcgc
     3601 acattccaac atgggtattc catcatgacc aaattcattt tcgtaaccgg cggcgttgtc
     3661 tcctcactgg gtaaaggtat cgccgccgct tctattgccg ctatcctcga atcgcgcggc
     3721 ttgaacgtta ccatgctcaa gcttgatcct tatatcaacg tcgatcccgg cacgatgagc
     3781 ccgtttcaac acggcgaagt gttcgtaacc gacgacggcg cggaaaccga cctcgactta
     3841 ggacactacg aacggtttat cgattccacg atgacccgcc gcaacagctt cagcacgggg
     3901 caggtgtacg aaaacgtcat cgccaaagaa cgacggggcg actacctcgg cggcacggtt
     3961 caagtcatcc cgcacattac cgacgaaatc aaacgccgca tccacgaagg cgcggcaggt
     4021 tacgatgtgg cgattgtcga aatcggcggc acggtcggcg acatcgaatc gctgccgttt
     4081 ttggaagcca tccgccagat gcgaagccag ttgggacgca acaacaccct gttcgcccac
     4141 ttgagctacg tcccctacat cgccgctgca ggcgaaatca aaaccaagcc gacccagcac
     4201 accgttaaag aaatgttgag catcggtttg caacccgaca tcctgatttg ccgtatggac
     4261 aggacaatgc ctgcggacga acgccgcaaa atcgccttgt tctgcaacgt ggaagaacgc
     4321 gcgattgtcg gcagctacga tgtggacagc atctacgaat gccccgaaat gctgcacgac
     4381 caaggcatcg acaacatcat taccgagcag ttgcagctta atgtgcagca ggcggatttg
     4441 accgcgtgga aaaaaatcgt ccacgccatc caaaacccga aacacaccgt caaaatcgcc
     4501 atggtcggca aatacgtcga tttgaccgaa tcctacaaat cactgattga agccttgaaa
     4561 cacgcaggtg ttcacaccga aaccgacgta caaattacct ttgttgacag cgaaagcatc
     4621 gagaaaaaca acggcgacgt ttctatgctc aaagatatgg acgccatcct cgttcccggc
     4681 ggtttcggtt cgcgcggcgt ggaaggcaaa atcgccgctg tgcgctatgc ccgcgaaaac
     4741 aacgtgccat acttgggcat ctgcctcggt atgcagattg cgctgatcga atacgcccgc
     4801 gacgtggcag gtttgaaagg tgcgaattcc accgagttcg acctcaaatg cgccgccccc
     4861 gtcgttgccc tgattgacga atggcaaact gccgacggca gcgtcgaaac ccgcgacgaa
     4921 tccgccgatt tgggcggcac catgcgtttg ggcgcgcaag aagtcgaatt gaaaacaggc
     4981 agcctcgccg ccaaaatcta cggcagcgga cacatccgcg aacgccaccg ccaccgctac
     5041 gaagtcaaca acaactatgt tcctacgctg gaacaggcag gtttggtcat cggcggcgta
     5101 tccgccggac gcgaacgctt ggtcgaaacc atcgaactgc cgaaccatcc ttggttcttc
     5161 gcctgccagt tccaccccga gtttacctcc aatccgcgca aagggcatcc tttgttcacc
     5221 gcgtttgtca aagctgcgtt gaacaataaa aaagcctgat aaagcgttac ttgatgatca
     5281 aaatgccgtc tgaaagcctg aatgaagact ttcagacggc attttcgcaa atcggggatt
     5341 ataaaagcca tcaatttaat ttttatggtt ttatctaata tttaccaact gaatattgtt
     5401 aagcggggcg tgaaactcaa gttgagacct ttgcaaaatt ccccaaaatc ccctaaattc
     5461 ccaccaagac atttaggagc accttcttcc ggcaaaccgc ccaagccatg attgccaaac
     5521 acatcgaccg tttcccgcta ttgaagttgg accgggtgat tgattggcag ccgatcgaac
     5581 aatacctgaa ccgtcaaa
//
LOCUS       DAXHTR010000135         5519 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.135, whole genome shotgun sequence.
ACCESSION   DAXHTR010000135 DAXHTR010000000
VERSION     DAXHTR010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5519
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.135"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..185
                     /locus_tag="ACFNML_001722"
     CDS             <1..185
                     /locus_tag="ACFNML_001722"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393063.1"
                     /translation="AAFIRSNRIRSAAIPTKVGIQFFEFQSFPINCLSIECLDSRLRG
                     NDDGNLVYIESKKPAP"
     gene            complement(246..3071)
                     /gene="lbpA"
                     /locus_tag="ACFNML_001723"
                     /gene_synonym="tbpA"
     CDS             complement(246..3071)
                     /gene="lbpA"
                     /locus_tag="ACFNML_001723"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HFC6393064.1"
                     /translation="MNKKHSFPLTLTALAIATAFPSYAANSETAAQTQSLKEVTVRAA
                     KVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYSIRGV
                     DKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAGSSDH
                     GSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGWEGLL
                     IRTERQGRETRPHGDIADGVEYGIDRLDAFRQTYDIQNPNKKAEYFLAEGESEPKPVA
                     KLAGNGKYLKNQLNRWVEERIEQNQPLSAEEEAMVREAQARHENLSAQAYTGGGRILP
                     DPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDESKKFSDKS
                     GVYDGDDFRDGLYFRPNIEEWKGDKNLVKGIGLKYSRTKFIDEHHRRRRMGLLYRYEN
                     EAYSDNWTDKAVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSDRFH
                     YREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASKAISRPEQLSHNAARISEHTGEYT
                     ADGKDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFDFS
                     LGGRYDRQNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSLSYRASSGFRTPSFQELF
                     GIDIYHDYPKGWQRPALKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADQKT
                     KLPDSTGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYHKLPEGLYTTLAYNRIKP
                     KSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAYLA
                     GDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAESTA
                     NRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3068..5287)
                     /locus_tag="ACFNML_001724"
     CDS             complement(3068..5287)
                     /locus_tag="ACFNML_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6393065.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAPTLSDSKS
                     SNPADKPAPAPAEPSVEITPVNRPAVGAAMRLPRRNTAFHREDGTVIPDSKQAEEQLS
                     FKEGDVLFLYGSKENKLQQLKSEIHKRDPDVEIRTSENENKKYDYKFVDAGYVYVKGK
                     DEIKWTSDHKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGK
                     AVGIDNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDKKILKGELIKNQYVRD
                     RNGPYNPLTIYDITATLDGNRFTGSAKVNTKLKTSHADKEYLFFHTDADQRLEGGFFG
                     DNGEELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPSGKHTKILDSLKISVDEA
                     ADKKARPFEVSPMPDFGHPDKLLVEGREISLVKDTQTIDLADGRKMIVRACCDFLTYV
                     KLGRIKTDRPASKPKAEDKGEDEEDTGVGNDEEGEDEIGDEEGTGDEAVGDEGSEENE
                     AVEDEGSEEDEGEEAEKPEEESPAEGGGGGSDGILPAPEAPKGRDIDLFLKGIRTAEA
                     DIPQTGEAHYTGTWEARIGKPIQWDNKADKAAKAEFDVDFGKKSISGKLTEKNGVEPA
                     FYIENGVIDGNGFHATARTRDNGINLSGNDSTNPQSFKANNLLVTGGFYGPQAEELGG
                     TIFNNDGKSLDITEDTENEAEVENEAEAGVDEQLKPEVKPQFGVVFGAKKDMQEVEK"
BASE COUNT         1192 a         1476 c         1273 g         1578 t
ORIGIN      
        1 ttgcggcatt tatcaggagc aacagaatcc gctctgccgc cattcccaca aaagtgggaa
       61 tccagttttt cgagtttcag tcattcccga taaattgcct tagcattgaa tgtctagatt
      121 cccgcctgcg cgggaatgac gacggaaatc ttgtttatat tgaatcaaaa aaacctgcac
      181 cttaatcagt tggcggttta gtccgacttt tggggtgcag atcaagcttt cagacggtat
      241 ttcctttaaa acttcatttc gagcgcgagg ctgaagttcc tgcccggtgc ggcatacctt
      301 ccatagttgc tgtcgccgcc gtgccggttt gccgtgcttt ccgcggtctg gcgcaaggat
      361 tcccaagtaa cgtagcggta gttgccgata ttgtagatag ccgccctcaa ggtcagccgt
      421 tttttcagat tcagataggc ggaaacgtct gccgtcgacc aagaagacga cgctcttttt
      481 gtcgaatatc gtttttgatc gcctgccaga taagcaagct cgtcagggtt tttccctttg
      541 gaataggtca gcataatgtt tgcgccccat ttcccctcgg gctggtcgta tccgaacccc
      601 aaaacataac gcgacggttg cacggcatcc aaagcatagc tgcggaggga cagtccaggc
      661 cggttggata cggatttcgg tttgatacgg ttgtacgcca aagtcgtgta caaaccttcg
      721 ggcagtttgt gatacacgcc gttccagtcg atttttccca agatgttgat gccttggagc
      781 gacatatttt gggcattgta ataatcgcgt atatcaatct ctgtcaatcg tcctgttgaa
      841 tccggcaatt tggttttttg atcggcaacg gcaatcatat cggtatagcg gttgcggaag
      901 ctgctgattt ccaaaaagcc gaagtcgccc ttccactgca aaccgatttc gcggttggct
      961 gccttttccg atttcagggc gggacgctgc cagcctttcg gataatcgtg ataaatatct
     1021 ataccgaaaa gctcttggaa tgaaggcgtt ctgaagccgc tggaggcgcg gtaagacaaa
     1081 gaaaaatgcc ggttcggttt gaacacgatg ccgctgttcc acgaacggtc aacataccgc
     1141 ccgctgcgga cgagttcttc cgacgtggtg aagttttgcc ggtcgtacct gccgcccaag
     1201 ctgaaatcaa aatatttgcc gattgaaaaa cggtcgttca aagaaatgtg gatattgctg
     1261 ccgttgattt ttcttggcac gcatttgcgg gaacgcaagg tttcgatgta gccgcagacc
     1321 gatccttcga cgacttcggg cttacccaaa cggtacttgt ctttgccgtc ggcggtgtac
     1381 tcccccgtgt gttccgaaat ccttgccgca ttgtgggaaa gctgttcggg gcgggagatc
     1441 gctttggaag catcgtaacc gaagcccaaa gtcagatggt gtttcgtcca tttgtttttc
     1501 agcgatttct caaacgaggc attcaaaaca ttatgctgtt cgcggtagtg gaagcggtcg
     1561 ctgctgtcgt aggaatacgg tttgtccgcc gacgcgcggc aagatttgtc cacggcagga
     1621 tacacggcgc aattcagctt cagcgtgttg ttgtcggttg ccacgccctg tttgtcaaac
     1681 gacaacaccg ccttatccgt ccagttgtca gaatatgctt cgttttcata acggtacagc
     1741 aaacccatac ggcggcggcg gtgatgttca tcaataaatt tggtgcggga atatttcaaa
     1801 cctatgccct tgaccaaatt tttatcgccc ttccactctt ctatattcgg cctaaaatac
     1861 aagccgtcgc ggaaatcgtc gccgtcgtac accccgctct tgtcgctaaa ctttttcgac
     1921 tcgtccgtac cgtaatactg tttttccgtc atatcgcgga tgtcgtaacg ctgtttggta
     1981 tcctcaaaca cgccgccgac ataatgcctg ccgccgaagc ggtagcccag cttggcaagc
     2041 caagagccgc tgcggtaatc catcggatcg ggcaatatcc tgccgccgcc cgtgtaagct
     2101 tgggcggaca ggttttcgtg gcgcgcctgc gcctcccgca ccatcgcttc ttcttcagca
     2161 cttaaaggct gattttgttc aatacgttct tccacccagc ggttgagctg gtttttcaaa
     2221 tatttcccgt tgcccgccaa ttttgccacg ggcttgggtt cactctcgcc ctccgccagg
     2281 aaatattccg cctttttatt cggattctga atatcgtatg tctggcggaa cgcgtccaaa
     2341 cggtctatgc cgtattccac cccgtccgca atatcgccgt gcgggcgcgt ttcccgcccc
     2401 tgacgctcgg ttcggattag cagcccttcc caaccgtcct tactgaaacc cgcgccgagc
     2461 gacttcataa attggcggtt tttactgccg taggcggttt ttgcctgtat cccccaactt
     2521 ttgccgtctg aaatcagatc tgccgcctct ttggtacgga aagcgaccgc gccgccgagt
     2581 gcgccgctgc cgtgatcgga tgaaccggca cctttgtcga tttccaccgt gctgatgttt
     2641 tcatattcga tttcgttgat tgcaccgctg ccgccgcgtc cgccgtatcc gctcaacgat
     2701 ccctgcacgg taaacgcctg tatttgggca acgccgtcaa ccgaaaccgc cacacggttt
     2761 ttatccacgc cgcgtatcga gtagccgccg ctcgcaccgt tgccctgttc gacaaccgcc
     2821 acgcccggat cgtagcgcgt caggtcgcgg ataccgagta cctgttcttt gttcaacgtt
     2881 tccgacgttt tgacgatttt gcccaaaccg gtcgcctctt tcgatcgccg tcccactttg
     2941 gcggcacgga cggtaacctc tttcagggat tgggtctgcg ccgccgtttc ggagtttgcc
     3001 gcatatgacg ggaaggcggt tgcaatagcc aaggcagtca gggtaagcgg aaaactgtgt
     3061 ttcttattca tttttccacc tcctgcatat ctttcttcgc accgaatacc acaccgaatt
     3121 ggggtttaac ttcaggtttt aactgttcgt caacaccagc ttcagcttca ttttcaactt
     3181 cagcttcatt ttcagtatct tcagttatat caagagattt cccatcatta ttgaaaatag
     3241 taccgcccaa ttcctccgcc tgcgggccgt aaaagccgcc tgttacaaga agattattgg
     3301 ctttgaaact ttgaggatta gtcgaatcat ttcccgaaag attgatgccg ttatcccgag
     3361 tgcgcgctgt cgcgtggaaa ccgttgccat caatcacacc gttttcaata tagaaagcag
     3421 gttctacacc gtttttctcc gttagctttc cggaaatcga tttcttgccg aaatcaacgt
     3481 caaattctgc ttttgccgct ttatccgcct tattatccca ttgaatgggt ttgccgatac
     3541 gcgcttccca agtgccggta tagtgtgctt ctccggtttg cggaatgtcg gcttccgccg
     3601 tgcggatacc tttcaggaaa aggtcgatgt ccctgccttt aggggcttcc ggagcgggca
     3661 ggatgccgtc tgaaccgcca ccgccgcctt ctgccggcga ttcttcttcg ggtttttcag
     3721 cttcttcacc ttcgtcttct tcgctgcctt cgtcttctac ggcttcgttt tcttcgctgc
     3781 cttcatctcc tacggcttca tctcccgtgc cttcttcatc gccgatttcg tcttcgcctt
     3841 cttcgtcgtt accaacgcct gtatcctctt catcctcccc tttatcttcc gcctttggtt
     3901 tacttgccgg gcggtcggtt tttatccgtc cgagtttcac ataggtcaga aaatcgcaac
     3961 aagcacggac gatcattttc ctgccgtcgg caagatcaat ggtttgagta tctttgacca
     4021 aggagatttc acgcccttcg acaagaagtt tgtcgggatg accaaaatcg ggcatagggg
     4081 aaacctcaaa cggacgggct tttttatcag ccgcctcgtc aacggaaatt tttagagaat
     4141 ccaagatttt ggtgtgtttt ccagacggca gggcaggatt tgtatctgat gcgtttgctg
     4201 tctctgtttt ttgtttgcct gcgaatacgc cgaatacgct tttgtcgttg ctgataaacc
     4261 gtccggcaag ctcttctccg ttatcaccga aaaaaccgcc ctcaagccgc tgatcggcat
     4321 cggtatggaa aaacaaatat tctttatcag cgtggctcgt cttcaacttg gtgttaactt
     4381 tggcactgcc ggtaaagcgg ttgccgtcca atgttgcagt aatgtcgtaa atggtcagcg
     4441 gattataggg accatttcta tctcgcacat actgattttt aatcagctcg ccctttagga
     4501 tttttttgtc gaaatcaacc gtatatttgg caggatgctt ttccctttcg tcgacatcct
     4561 tagccgcata agaagttgca ccaacatcgt taccataaaa tgtgatataa cccaaattgt
     4621 caatgccaac cgccttacct gctcgatgac gtttgacatc ggtcatatat tgccagttac
     4681 cagaatattc caccgttccc gcactcggta aagattggga aggacgttct ccggaataat
     4741 atacaaaacc gtcataacct aagcggttgg aaaactgctt gtgatctgaa gtccacttaa
     4801 tttcatcttt tccctttaca tatacataac ctgcatctac aaatttataa tcatattttt
     4861 tattttcatt ttctgatgtc ctaatttcta catcgggatc acgtttatga atttcgcttt
     4921 taagttgttg aagtttattt tcttttgaac cgtataaaaa cagaacatca ccttctttaa
     4981 acgacagctg ttcttctgct tgtttgctat cgggaattac cgtgccatct tcacgatgaa
     5041 aagcagtatt ccgccttggc agccgcattg ccgcaccgac ggcgggccgg ttgaccggcg
     5101 tgatttctac cgaaggctcg gcaggagctg gagcaggctt atccgcagga ttggaagatt
     5161 tggaatctga cagagttggc gcggtcggcg ttgattcgac aacaggctgt acgccgaaat
     5221 tgccgccgat acaagatgcc aaaagtaagg gcaacaagac aatgccgcca taattcggtt
     5281 tacacatccc tacttttcct cctatttgat taataataat tatcattata ttaatatgta
     5341 cagataatat caagccgttt ttatagtgaa ttaacaaaaa tccggacaag gcgacgagcc
     5401 gcagacagta cagatacatc ccgtcattcc cacgaacctg catcccgtca ttcccacgaa
     5461 cctacattcc gtcattccca cgaaagtggg aatccagttc gttcggtttc gcttgtttt
//
LOCUS       DAXHTR010000136         5360 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.136, whole genome shotgun sequence.
ACCESSION   DAXHTR010000136 DAXHTR010000000
VERSION     DAXHTR010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.136"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            198..2315
                     /locus_tag="ACFNML_001725"
     CDS             198..2315
                     /locus_tag="ACFNML_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6393066.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGGSFDLDSVDTEAPRPAPK
                     YQDVSSEKPQAQKDQGGYGFAMRLKRRNWYPSAKENEVKLNESDWEATGLPTDPKELP
                     KRQKSVIEKVETDDDSNIYSSPYLTPSNHQSGSAGNGVNQPKNKATNHENFQYVYSGW
                     FYKHAKQHRDLTNKIVQQGDDGYIFYHGKEPSRQLPASGKITYKGVWHFATDVKKSQN
                     FREIIQPSKSQGDRYSGFSGDDGEEYSNKNETTLQSGHEGYGFTSNLEVDFDNKKLTG
                     KLIRNNANTSNNQATTQYYSLEAQVTGNRFNGKATATDKPQNSETKEHPFVSDSSSLS
                     GGFFGPKGEELGFRFLSDDKKVAVVGSAKTQDKPENGAAALGGAGAAASGGVAAMPSE
                     NGKLTTVLDAVELTHGGTAIKNLDNFSNAAQLVVDGIMIPLLPKDSESGNNQANQGTN
                     GGTAFTYKTTYTPKSDEKDTQAGTAENGNPAASNTAGDTNGKTKTYEVEVCCSNLNYL
                     KYGLLTRKTAGNTGEGGNGSPTAAQTGAQSMFLQGERTDEKEIPNEQNVVYRGSWYGH
                     IANGTSWSGNASDKEGGNRAEFTVNFGEKKINGTLTAENRQAATFTIEGTIQDNGFSG
                     TAKTADLGFDLDQSNTTGTPKAYITNAKVQGGFYGPKAEELGGWFAYPGDSQAQPSAS
                     GSGTSAANSATVVFGAKRQQLVQ"
     gene            2402..5149
                     /gene="tbpA"
                     /locus_tag="ACFNML_001726"
     CDS             2402..5149
                     /gene="tbpA"
                     /locus_tag="ACFNML_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HFC6393067.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEAAGRGVQSFNRLVPVEDTNQYANFIVEEECESGNYETCKANP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILERT
                     QQTFDTRDMTVPAFLTKAVFDENKKQAGSLSGNGKYAGDHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYYQNANRAYSSNLPTQNGNQNISPNGSQDKPYWVSIGGGNVVTGQICRLGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGVVLKPFTWMDLTYRTSTGFRLPSFSEMYGWRSGDKIKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIKNGKEEAKGDPAYLNAQSARITGINILG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNHRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
BASE COUNT         1522 a         1345 c         1385 g         1108 t
ORIGIN      
        1 gatatacgtg ggaatgacgg gatatacgtg ggaatgacgg gatataggtt tccgtgcgga
       61 cgcgttcgga ttcacgactg cgcgggaatg acgggatttt ggtgtattcc ctaaaaaaaa
      121 taaaaaaagc atttgcaaat ttattaaaaa taaataaaat aataatcttt atcattcttt
      181 aattgaatta ggtttatatg aacaatccat tggtaaatca ggctgctatg gtactgcctg
      241 tgtttttgtt gagtgcttgt ttgggcggag gcggcggcag tttcgatctt gattctgtcg
      301 ataccgaagc cccgcgtccc gcgccaaagt atcaagatgt ttcttccgaa aaaccgcaag
      361 cccaaaaaga ccaaggcgga tacggttttg cgatgaggtt gaaacggagg aattggtatc
      421 cgtcggcaaa agaaaacgag gttaaactga acgagagtga ttgggaggcg acaggattgc
      481 cgacagaccc caaggaactc cctaaacggc aaaaatcggt tattgaaaaa gtagaaacag
      541 acgacgacag caatatttat tcttccccct atctcacacc atcaaaccat caaagcggca
      601 gcgctggcaa cggtgtaaat caacctaaaa ataaggcaac aaatcacgaa aatttccaat
      661 atgtttattc cggttggttt tacaaacacg ctaaacaaca tcgtgatttg acaaataaaa
      721 ttgtacaaca aggcgacgac ggttatatct tctatcacgg taaagaacct tcccgacaac
      781 ttcccgcttc tggaaaaatt acctataaag gtgtgtggca ttttgcgacc gatgtcaaaa
      841 aatctcaaaa ttttcgtgaa attatccaac cttcaaaaag tcaaggcgac aggtatagcg
      901 gattttcggg tgatgacggc gaggaatatt ccaataaaaa tgaaacgact ttacaaagcg
      961 gtcacgaggg ttatggattt acctcgaatt tagaagtgga tttcgacaat aagaaattga
     1021 cgggtaaatt gatacgcaac aatgcgaata ccagtaacaa ccaagccacc acccaatact
     1081 acagccttga ggctcaagta acaggcaacc gcttcaacgg caaggcaacg gcaaccgaca
     1141 aaccccaaaa cagcgaaacc aaggaacatc cctttgtttc cgactcgtct tctttgagcg
     1201 gcggcttttt cggcccgaag ggtgaggaat tgggtttccg ctttttgagc gacgataaaa
     1261 aagttgccgt tgtcggcagc gcgaaaaccc aagacaaacc ggaaaatggc gcggcggctt
     1321 taggcggcgc aggtgcggca gcatcgggcg gtgtggcagc tatgccgtct gaaaacggta
     1381 agctgaccac ggttttggat gcggtcgagc tgacgcacgg cggcacagca atcaaaaatc
     1441 tcgacaactt cagcaacgcc gcccaactgg ttgtcgacgg cattatgatt ccgctcctgc
     1501 ccaaggattc cgaaagcggg aacaatcaag ccaatcaagg tacaaacggc ggaacagcct
     1561 ttacctacaa aacaacctac acgccgaaaa gcgatgaaaa agacacccaa gcaggtacgg
     1621 cggagaatgg caatccagcc gcttcaaata cggcaggtga taccaatggc aaaacaaaaa
     1681 cctatgaagt cgaagtctgc tgttccaacc tcaattatct gaaatacggg ttgctgacgc
     1741 gcaaaactgc cggcaatacg ggggaaggcg gcaacggcag cccaaccgcc gcccaaacgg
     1801 gcgcgcagag tatgttcctt caaggcgagc gcaccgatga aaaagagatt ccaaacgagc
     1861 aaaacgtcgt ttatcggggg tcttggtacg gacatattgc caacggcaca agctggagcg
     1921 gcaatgcttc agataaagag ggcggcaaca gggcggaatt taccgtgaat tttggcgaga
     1981 aaaaaattaa cggaacgtta accgctgaaa acaggcaggc ggcaaccttt accattgagg
     2041 gaacgattca ggacaacggt ttttccggta cggcgaaaac tgctgactta ggttttgatc
     2101 tcgatcaaag caataccacc ggcacgccta aggcatatat cacaaacgcc aaggtgcagg
     2161 gcggttttta cgggcccaaa gccgaagagt tgggcggatg gtttgcctat ccgggcgaca
     2221 gtcaggcgca gccgtccgct tcggggtcag gtacgtcagc cgccaacagc gcgaccgtgg
     2281 tattcggtgc gaaacgccaa cagcttgtgc aataagcacg gctgccgaac aatcgagaat
     2341 aaggcttcag acggcatcgc ttcttccgat gccgtctgaa agcgaagagt agggaaacac
     2401 tatgcaacag caacatttgt tccgattaaa tattttatgc ctgtcgctga tgaccgcgct
     2461 gcccgtttat gcagaaaatg tgcaagccgg acaagcacag gaaaaacagt tggataccat
     2521 acaggtaaaa gccaaaaaac agaaaacccg ccgcgataac gaagtaaccg gtttgggcaa
     2581 attggtcaaa accgccgata ccctcagcaa ggaacaggta ctcgatatcc gcgacctgac
     2641 gcgttacgac cccggcatcg ccgtggtcga acaggggcgt ggcgcaagct cgggctattc
     2701 gatacgcggt atggacaaaa accgcgtttc cttgacggtg gacggcttgg cgcaaataca
     2761 gtcctacacc gcgcaggcgg cattgggcgg gacgaggacg gcgggcagca gcggcgcaat
     2821 caatgaaatc gagtatgaaa acgtcaaggc tgtcgaaatc agcaaaggct caaactcggt
     2881 cgaacaaggc agcggcgcat tggcgggttc ggtcgcattt caaaccaaaa ccgccgacga
     2941 tgttatcggg gaaggcaggc agtggggcat tcagagtaaa accgcctatt ccggcaaaaa
     3001 ccgggggctt acccaatcca tcgcgctggc ggggcgcatc ggcggtgcgg aggctttgct
     3061 gatccacacg ggtcggcgcg cgggggaaat ccgcgcccac gaagccgccg gacgcggcgt
     3121 tcagagcttt aacaggctgg tgccggttga ggataccaat cagtacgcaa atttcatcgt
     3181 tgaagaagaa tgcgaaagcg ggaattacga aacgtgtaaa gcgaatccga aaaaagatgt
     3241 tgtcggcaaa gacgaacgtc aaacggtttc tacccgagac tacacgggtc ccaaccgctt
     3301 tctcgccgat ccgctttcat acgaaagccg gtcgtggctg ttccgcccgg gttttcgttt
     3361 tgagaataag cggcactata tcggcggtat actcgaacgc acgcaacaaa ctttcgacac
     3421 gcgcgatatg acggttccgg cattcctgac caaggcggtt tttgatgaaa ataaaaaaca
     3481 ggcgggttct ttgtccggca acggcaaata cgcgggcgac cacaaatacg gcggactgtt
     3541 taccaacggc gaaaacggtg cgctggtggg cgcggaatac ggtacgggcg tgttttacga
     3601 cgagacgcac accaaaagcc gctacggttt ggaatatgtc tataccaatg ccgataaaga
     3661 cacttgggca gattatgccc gcctctctta cgaccgtcag ggcatcggtt tggacaatca
     3721 ttttcagcag acgcactgtt ctgccgacgg ttcggacaaa tattgccgtc cgagtgccga
     3781 caagccgttt tcctactaca aatccgatcg cgtgatttac ggggaaagcc acaggctctt
     3841 gcaggcggca ttcaaaaaat ccttcgatac cgccaaaatc cgccacaacc tgagcgtgaa
     3901 tctcggtttt gaccgctttg gctctaatct ccgccatcag gattattatt atcaaaatgc
     3961 caaccgcgcc tattcgtcga atctgcccac tcaaaacggc aatcaaaaca tcagccccaa
     4021 cggcagccaa gacaaaccct attgggtcag cataggcggg ggaaatgtgg ttacggggca
     4081 aatttgccgc ttgggcaata atacttatac ggactgcacg ccgcgcagca tcaacggcaa
     4141 aagctattat gcggcagttc gggacaatgt ccgtttgggc aggtgggcgg atgtcggcgc
     4201 ggggttgcgc tacgactacc gcagcacgca ttcggacgac ggcagcgttt ccaccggcac
     4261 gcaccgcacc ctgtcttgga acgcgggtgt agtcctcaaa cctttcactt ggatggattt
     4321 gacttaccgc acttcaaccg gcttccgcct gccctcgttt tcggaaatgt acggctggcg
     4381 gtcgggcgat aaaataaaag ccgtcaaaat cgatccggaa aaatcgttca acaaagaagc
     4441 cggcatcgtg tttaaaggcg atttcggcaa cttggaggca agttggttca acaatgccta
     4501 ccgcgatttg attgtccggg gttatgaagc gcaaattaaa aacggcaaag aagaagccaa
     4561 aggcgacccg gcttacctca atgcccaaag cgcgcggatt accggcatca atattttggg
     4621 caaaatcgat tggaacggcg tatgggataa attgcccgaa ggatggtatt ccacatttgc
     4681 ctataatcgt gtccgtgtcc gagatattaa aaaacgcgca gaccgcaccg atattcaatc
     4741 acatttgttt gatgccatcc aaccctcgcg ctatgtcgtc ggcttgggct atgaccaacc
     4801 ggaaggcaaa tggggtgtga acggtatgct gacttattcc aaagccaagg aaatcacaga
     4861 gttgttgggc agccgggctt tgctcaacgg caacagccgc aatacaaaag ccaccgcgcg
     4921 ccgtacccgc ccttggtata ttgtggatgt gtccggttat tacacgatta aaaaacactt
     4981 taccctccgt gcgggcgtgt acaacctcct caaccaccgc tatgttactt gggaaaatgt
     5041 gcggcaaact gccggcggcg cagtcaatca acacaaaaat gtcggcgttt acaaccgata
     5101 tgccgccccc ggccgaaact acacatttag cttggaaatg aagttttaaa cgtccaaacg
     5161 ccgcaaatgc cgtctgaaag gcttcagacg gcatttttta cacaattccc gccattttcc
     5221 atcatccccg atacaccgta atcctgaaac ccgtcattcc cgcgcaggcg ggaatccagt
     5281 ccgttcggtt tcggtttttt gaggtttcgg gtaacttcta aaccgtcatt cccgcgaagg
     5341 cggaaaatca aaaacagaaa
//
LOCUS       DAXHTR010000137         5269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.137, whole genome shotgun sequence.
ACCESSION   DAXHTR010000137 DAXHTR010000000
VERSION     DAXHTR010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.137"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            771..896
                     /locus_tag="ACFNML_001727"
                     /pseudo
     CDS             771..896
                     /locus_tag="ACFNML_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(1047..2090)
                     /gene="argC"
                     /locus_tag="ACFNML_001728"
     CDS             complement(1047..2090)
                     /gene="argC"
                     /locus_tag="ACFNML_001728"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFC6393068.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSKAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEVGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGICPETILRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLGAIPLLP"
     gene            complement(2087..2380)
                     /locus_tag="ACFNML_001729"
     CDS             complement(2087..2380)
                     /locus_tag="ACFNML_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393069.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            2634..3788
                     /locus_tag="ACFNML_001730"
     CDS             2634..3788
                     /locus_tag="ACFNML_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6393070.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDPRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHFA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTATQIAAMIADPVNYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKYEEVRKLIYQLIELDFLEIL"
     gene            3797..4024
                     /locus_tag="ACFNML_001731"
     CDS             3797..4024
                     /locus_tag="ACFNML_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393071.1"
                     /translation="MKNWKQFTFFVILVIACYQVLYFLSDMFLLDYINKYSWNLNFIQ
                     GTLNFFSIYLPYVFVSRIFRNTNQEKEYKND"
     gene            4017..4370
                     /locus_tag="ACFNML_001732"
     CDS             4017..4370
                     /locus_tag="ACFNML_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393072.1"
                     /translation="MIELQLHELKLVSGGGPVTDNIAGNVANAATTKGGPTWGDLVAI
                     PAAAAAVYYLPKNAYGAAGANGVYNVTRNWVNDAVNAPPYNGRPIFEIEHGLTAPATK
                     ADKSGNGYTDGTDYC"
     gene            4431..5057
                     /locus_tag="ACFNML_001733"
     CDS             4431..5057
                     /locus_tag="ACFNML_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HFC6393073.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHENETRSLKATVERLENQKLH
                     ILQQIDGQKRRIRLAEDQWQKRHWADRNCRDWAWYPPIWRRATNLFIS"
     gene            4961..5209
                     /locus_tag="ACFNML_001734"
                     /pseudo
     CDS             4961..5209
                     /locus_tag="ACFNML_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFP transporter"
BASE COUNT         1435 a         1153 c         1368 g         1313 t
ORIGIN      
        1 tttttgacac attttcatga tttcaaatcc cgttattccc aagtggacgg aaacccgata
       61 atgggaagcc gcagagactt atcgggaaaa cggcaagccc tccgccgtca ttcccgcgca
      121 ggcgggaatc tagaaataaa aaaccacggt gttatcggca atgactgaaa ccggagaaat
      181 gacgaaattg aagcgtgcgg aaatgtgtcg gaaatcgctg aagccggata aactagattc
      241 ccgcttttgc gggaatgacg ggattttagg tttttggttt gttttcgcgg caatgatggg
      301 attttaggtt tttggtttgt tttcacggca atgatgggat tttaaattgc aggtatttac
      361 cgggaaaaac ggcaagctct ccgccgtcat tcccgcgcag gcgggaatct agaaatgaaa
      421 aaccacggtg ttatcggaaa tgattgaaac cggagaaatg acggaatcga agcgtgtgga
      481 aatgtgtcgg aaatcgctga agccggataa actagattcc cgcttttgcg ggaatgacgg
      541 gattttaggt ttttggtttg ttttcgcggc aatgacggga ttttaggttt ttggtttgtt
      601 ttcacggcaa tgatgggatt ttaaattgca ggtatttacc gggaaaaacg gcaagccctc
      661 cgccgtcatt cccgcgcagg cgggaatcta gaaatgaaaa accacggtgt tatcggcaat
      721 gactgaaacc ggagaaatga cgaaattgaa gcgtgtggaa atgtgtcgga aatcgctgaa
      781 gccggataaa ctatagtgga ttaaatttaa atcaggacaa ggcgacgaag ccgcagacag
      841 tacagatagt acggcaaggc gaggcaacgc tgtactggtt taaatttaat ccactataga
      901 ttcccgcttt tgcgggaatg acggtatttt agatttttgt tttcgcggaa taatgagcca
      961 aaagccgtcc gaacccaaaa aaaccgtcgc tttcagacgg catcaaaaaa ccgcaggcac
     1021 gatgcctgcg gtttgccgtt tgcgcttcag gggagcaggg ggattgcgcc caagccgtgt
     1081 gtttccttca gtccgaacat gatgttcata ttttgcaccg cctgacccgc cgcgcctttg
     1141 acgaggttgt cgatgacgga aaggacgacc cacacatcgg attgcgccgc ctgttggatg
     1201 ctgatgcggc agaggtttgc gccgcgcacg ctgcgggttt cgggtgtcga accggcgggc
     1261 aggatgtcca cgaacaggct gtcgcggtaa tagtcgcgca ggatggtttc ggggcagatg
     1321 ccgtctgaaa ggtggaggta aacggtggcg tgcataccgc gtatcatcgg cgcgaggtgc
     1381 ggcgtgaaca cgaatccttc ggcgatgccg tcctgaagcc cggcgatggt ctgcctgatt
     1441 tcgggcaggt ggcggtgtcc agctatgccg tatgctttga agttgtcgcc gacttcgcac
     1501 aacagcgaac cgacattgcc tttcctgccc gcgccggaca cgccggattt gcagtcggca
     1561 atcagcggca taccgggctt cagacggcat tgccgcaaca gcggcacgag cggcagggat
     1621 acgcaggtcg ggtagcagcc ggggttggcg acgaggcgtg cctgtgcgac ggcttcgcgg
     1681 ttgagttcgc tcaatccgta cacggcttgg gaaacgaggt cgggggcggc gtgggtcatg
     1741 ccgtaccagt gttcccaggt cggaatgtcc cgtatgcgga agtcggcgga aaggtcgatg
     1801 acgcgcacgc cctgttcaat caggcgcggc gcgtctttca tggcgatgcc gttgggcgtg
     1861 gcgaagaaga cgatgtcgca ttgttccaaa cctgcctcgt cgggcgtttg gaaggcgagg
     1921 ccgtacacgc cgcgcaaact cggaaagtaa tcggcaactg cagttcccgc tttgctgcgg
     1981 ctggttacgg cggcgacttc gacatcggga tgggcggcaa gcaggcgcag cagttccacg
     2041 cccgtgtagc ccgtcgcgcc gacaatgccg actttgattt ttttactcat ggtttttcct
     2101 ttgtgtggtt ggcgggtatg ccgtccgaac gctgtccgac ggcttgtccg acggggtttt
     2161 gattggcggc ggaaggacgg tacacaaagc gtttgaaaag cgtgtctaat ttaatggcat
     2221 ccgcctctgt ttttaaaacc cagccctgcc gtccggaata aacataaccg taggcgcgaa
     2281 agtcggcagg atatttcggt gcctgcccca aacccaaacc gtatgcggcg aaagcggtgg
     2341 tcgtggagaa ggaaagcagg aggaggacga gggttttcat aaggttccaa accgaacggg
     2401 gcgatgaagc ccgattatag caaaaggtcg tctgaaaacc gttttcagac gaccttttat
     2461 ttaattacaa taaaaatgtt aacaacaaaa aacaaaccgc ttttttccgt ttggagacaa
     2521 atttttaact aaaattgtca acaattcctt gacgcacaca caaaaaaaac tttaatattt
     2581 gtaaatatat gtaacattat aaattttaaa tattttctga gtaaggggaa gtaatgagca
     2641 tccatctcga cttcggcatt agtcctaaaa cgttccgaca gacttatctg tatcaaaagc
     2701 ccaagctctt taaaggagcg gttcggaatc tcgaatccgc atcttggaaa gatatcaacg
     2761 agatatacca acgagcagac ccaaccgcac cgctgtttca tctgcgtaaa aaaggcgcaa
     2821 tcgttcctaa agaagaatac gtcgaaagtt tcgacgattt gggcaaaact cgctaccgtt
     2881 ttattaaatc cgttatctac gaacatatga agaatggtgc gtcgttagtc tataaccata
     2941 ttaacaacga gccgttttca gaccatatcg cccgtcaagt cgcccgcttt gccggcgcac
     3001 atactattgt tagtggatat cttgcttttg gcagcgacga atcttataaa aaccattggg
     3061 acccccgcga tgtgtatgcc atccagcttt tcggcaagaa acgttggcaa cttactgccc
     3121 ctgatttccc tatgccattg tatatgcaac agactaaaga tactgatatt tccattcctg
     3181 aacatatcga tatggatatt atccttgaag caggtgatgt cctctacatc ccacgcggtt
     3241 ggtggcacag acctatcccg ctcggctgtg aaaccttcca cttcgctgtc ggtaccttcc
     3301 caccaaacgg ctataattac ctcgagtggc taatgaagaa atttcccacc atagaaagtc
     3361 tgcgccacag tttctcagac tgggagcaag ataggacgcg tatcaacgat actgccacac
     3421 aaattgctgc catgattgcc gaccccgtca attatgaagc cttcagtgaa gactttctcg
     3481 gcaaagaacg taccgatacc gcttttcatc tcgaacagtt cgcgaatccc aacgctactc
     3541 cgctttcaga cgacgtcagg ttgagattaa atgccaataa tttggatacg ttggaaaagg
     3601 gatatttgat tgggaatggg atgaagataa gcgtagatga gttggggaaa aaagtgttag
     3661 aacacatcgg taagaatgaa ccgttattgt tgaaaaatct actggttaac ttcaatcagg
     3721 gaaaatatga agaagttagg aagttgattt atcagttgat agagttagat tttctggaaa
     3781 ttttgtgagg gattctatga aaaactggaa gcaatttaca tttttcgtaa tattagtaat
     3841 agcttgttat caagtgctat attttttatc agatatgttt cttctcgact acattaacaa
     3901 atatagttgg aatttgaatt ttattcaggg tactttgaat tttttttcaa tatatctgcc
     3961 atatgttttt gttagccgaa tttttaggaa taccaaccaa gaaaaggagt ataaaaatga
     4021 ttgaacttca acttcatgaa ttgaagctgg tttcaggggg aggtcctgta acagacaata
     4081 tagctggaaa tgtagctaat gctgccacaa ccaaaggagg tcccacatgg ggggatttgg
     4141 ttgcaatacc tgctgccgca gcggcagttt attatctgcc gaaaaatgct tacggtgctg
     4201 ctggtgcaaa tggtgtatac aatgtgactc gtaattgggt aaatgatgcc gttaatgcac
     4261 ctccttataa cggaagacca atctttgaga ttgaacatgg attaactgct cccgcaacaa
     4321 aagcagataa atcaggaaac ggctacactg acggtacaga ttactgctga tatttctcat
     4381 cgtccagaca atctctaggg gtcgtctgaa actttcttaa cttcaatttt atgaatagac
     4441 ccaagcaacc cttcttccgt cccgaagtcg ccgttgcccg ccaaaccagc ctgacgggta
     4501 aagtgattct gacacgaccg ttgtcatttt ccctatggac gacatttgca tcgatatctg
     4561 cgttattgat tatcctgttt ttgatatttg gtaactatac gcgaaagaca acagtggagg
     4621 gacaaatttt acctgcatcg ggcgtaatca gggtgtatgc accggatacg gggacaatta
     4681 cagcgaaatt cgtggaagat ggagaaaagg ttaaggctgg cgacaagcta tttgcgcttt
     4741 cgacctcacg tttcggcgca ggaggtagcg tgcagcagca gttgaaaacg gaggcagttt
     4801 tgaagaaaac gttggcagaa caggaactgg gtcgtctgaa gctgatacac gagaatgaaa
     4861 cgcgcagcct taaagcaact gtcgaacgtt tggaaaacca gaaactccat attttgcaac
     4921 agatagacgg tcagaaaagg cgcattagac ttgcggaaga tcagtggcaa aaacggcact
     4981 gggcagacag gaattgtcgg gattgggcat ggtatcctcc gatttggcga agagcaacga
     5041 acctgtttat ctcgtgaaaa taaaacccga caaaccaacc atcactgcat acggtgagga
     5101 aaaaccgctg caaatcggca tgacgttgga agcagacatc ctacacgaga aacggcgatt
     5161 gtacgaatgg gtattggagc tgatttatag tatgtcgggc aaactgtaag acttgaggct
     5221 gtaaagataa agaagttgtc tgaaaccttt tcagacgacc agaagacaa
//
LOCUS       DAXHTR010000138         5264 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.138, whole genome shotgun sequence.
ACCESSION   DAXHTR010000138 DAXHTR010000000
VERSION     DAXHTR010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.138"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            113..808
                     /gene="pgl"
                     /locus_tag="ACFNML_001735"
     CDS             113..808
                     /gene="pgl"
                     /locus_tag="ACFNML_001735"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFC6393074.1"
                     /translation="MFVWHEYENAAEAAQSLADAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIRGEEKKAVFDQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            789..1775
                     /locus_tag="ACFNML_001736"
     CDS             789..1775
                     /locus_tag="ACFNML_001736"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFC6393075.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            1825..2673
                     /gene="hexR"
                     /locus_tag="ACFNML_001737"
     CDS             1825..2673
                     /gene="hexR"
                     /locus_tag="ACFNML_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFC6393076.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            2794..4440
                     /gene="pgi"
                     /locus_tag="ACFNML_001738"
     CDS             2794..4440
                     /gene="pgi"
                     /locus_tag="ACFNML_001738"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFC6393077.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGSQKHDSSTERLINLYLQTNRK"
     gene            4583..5005
                     /locus_tag="ACFNML_001739"
     CDS             4583..5005
                     /locus_tag="ACFNML_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFC6393078.1"
                     /translation="MALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEVDRVSEWEE
                     RYKLSRSEFEQLWKGLPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSEL
                     ACKTVETEARLEELYNRKKALIDEMVREADKKELPKRL"
BASE COUNT         1341 a         1465 c         1393 g         1065 t
ORIGIN      
        1 aaagcagtcc cgatatttgg tatcaaaagc ataaacaact gttcgaccgg catattgctc
       61 aacccgtttc cataacggaa tatgccgtct gaaataaaaa aaggacacaa atatgtttgt
      121 ttggcacgaa tacgaaaatg cggcagaagc ggcgcagtct ttggctgacg cagtggcgga
      181 tgctttgcag ggcgcactgg acgagaaggg cggtgcggtg ttggcagttt ccggcggacg
      241 ttcgccgatt gcatttttca acgccctgtc gcaaaaagat ttggattgga aaaacgtcgg
      301 catcaccttg gcagatgaac gcatcgtgcc gaccgtccac gccgacagca ataccggttt
      361 ggtgcgcgaa tacctgttga agaacaaagc ggaagcggca atgtggattc ctatggtgga
      421 agacggaaaa actgaaaccg aattacatcc cgatgctgtt gtcgattatg cactgaaaca
      481 ttacaaacag cccgatgttt tggttttggg tatgggaaac gacgggcata cggcttcgat
      541 tttcccgaaa gctccgcagt ttcagacggc aatcgacggt tcggcaggtg tcgcgctggt
      601 gcataccacg cccgttaccg cgccgcacga gcgcgtcagt atgaccttgg atgcgattgc
      661 ccatacgggg catgtgtttt tggcgatacg gggcgaagag aaaaaagccg tgttcgacca
      721 agccgcacaa ggcgaaaacc gcgaatatcc gatcaacctc gttttgaacc atcaaggagt
      781 gaactgccat gtcttctacg ccgaataaac aagccggata tccccgactg gtcgccgata
      841 tcggcgggac gaatgcacgc tttgcgctgg aaaccgcgcc gcgcgtcatt gaaaaagccg
      901 ccgtgcttcc gtgtaaagac tacgatacgg ttaccgatgc ggtgcgtgcc tatctgaatc
      961 aaagcggtgc aacagccgta cggcacgcgg catttgccat cgccaacccg attttgggcg
     1021 actgggtgca gatgaccaac caccattggg cgttttccat cgaaaccacc cgtcagactt
     1081 tggggctgga caccctcatc cttttgaacg actttaccgc gcaggcattg gcggtaacgc
     1141 agacttcaag caaagacctg atgcaggtag gcgggcaaaa gcctgtcgaa tttgccccca
     1201 aagccgttat cggccccggt accggcctgg gcgtgagcgg attggtgcac agccacgcag
     1261 gctgggtggc tttggcgggc gagggcgggc ataccagttt cccgccgttt gacgatatgg
     1321 aagtgctgat ttggcagtac gccaaaaaca aatacggcca tgtttccgcc gaacgctttt
     1381 tgagcggcgc gggcttgagc ttggtttacg aggctttggc tgcaaaacag aaagccaaac
     1441 ccgccaaact gatgccgtct gaaatcacgg aaaaggcttt gagcggcgcg tcgcctttgt
     1501 gccgtcagac tttggacatc ttctgcgcca tgctcggcac ggttgcttcc aacctcgccc
     1561 tgacgctggg cgcgcgcggc ggcgtgtacc tgtgtggcgg cattattccc cgcgtgttgg
     1621 aatatttcaa aacttccccg ttccgcagcc gtttcgagaa caagggcagg tttgaagcat
     1681 atcttgccgc gattcccgtg tatgtcgtct tgagcgagtt tcccggaatt tctggtgcgg
     1741 ctgcggctct cgacaaccat ttgagaaacg tttaaccaca gcggctcctt gcagcggggc
     1801 tgcattatcg aagggcatat cattatgtta agcaaaatca gcgaatcact ggcaaacctt
     1861 tccggtgcgg aacgcaaagt cgccgaatgt gcattggcgg aacccaaatg gttcgtccat
     1921 gccgccgttg ccgaaattgc cgaacgtgcg tccgtcagcc agccgaccgt catccgattc
     1981 tgccgcagct tgggttataa agggctgccc gagttcaagc tcgcgttgtc cgccagcatc
     2041 ggtcatgagg gtatgcccta tgtccatgaa gaactcaacg ccgacgacga tatggcaagc
     2101 gtggtcgaga aagtgttggg caatgccgcc gcctcgctgt tgggcgaacg ccgcttcctg
     2161 aaagagtcgg agctggaaaa cgccattgcc acgctgatgc acgcccgccg cgtcgagttt
     2221 tacggtgtcg gcaattccgg tatcgttgcg caggacgcgc aacacaagtt tttccgtttc
     2281 ggaatgtcca cggtcgccta tgtcgatacg cacacgcagc tgatggcagc atccgttttg
     2341 agcgatcagg atgttttggt tgccatttcc aacacgggtt cgtctatcga acttttggat
     2401 gcggtcagca tcgccaaaga aaacggcgcg tccgtcatcg cactgacccg caacgattcg
     2461 cctctggcgc aacttgccga ctgcgtgttg agcgttgcca cacaggaaaa tgccgaactc
     2521 tacacgccca tggtttcccg cctcttgcag cttgccgtca tcgacattct cgccatcgga
     2581 cttgccctgc gcttgggcga tgctgccagc ctgcaattgc agaaaagcaa aaaaagcata
     2641 cacaacaagc acatcgatta cgacaaagat tgaccttcag acggcatccc acaaatgccg
     2701 tctgaaatgc cgaacaacgg ccgtcggcgg cttgcggcag tttccagcag ccttttcatc
     2761 ccacaacaaa aaacctcatt caggagcata tagatgaaac actttcacga cttacccgca
     2821 tggtcgaaat tgtggaacca ctttgacgac agcaaaacat tgcatatgcg cgaaatgttc
     2881 gagcaagacc cgcagcgtgc ggaacgctac tggctgcaag tcggcggact gacgctggac
     2941 tactccaaaa accgcatcaa cgacgaaacc atgtcgcttt tgttcgagct tgcccgagaa
     3001 gcaggcgtgc cggagcggat gcggcagatg ttccacggcg aaaaaatcaa taccaccgaa
     3061 aaccgcgccg tcctgcatgt cgcccttcgc aaccgcacca attcgccgat tgtggttgac
     3121 ggtgaagatg tgatgcccaa agtcaaccgc gttttgcaac gtatgggcga atttgcacac
     3181 gaagtccgca gcggaagctg gctgggctat accaaccaag tcattaccga cgttgtcaac
     3241 atcggcatcg gcggatcgga tttgggcccg ctgatgatgt gtaccgcgct caaacctttc
     3301 ggtcatccgc gcctcaatat gcacttcgtc tccaacgtgg acggctccca actgcgcgac
     3361 gtattgtcca aagtccaccc cgaaaccacg ctgttcatca tcgcctccaa aacatttacc
     3421 acgcaggaaa cgctgaccaa cgcgctgacc gcgcgcgaat ggtttttgaa tcatgcgggc
     3481 gacgaagaag ccgttgccaa acacttcgcc gccgtttcca ccaatcaaaa agccgtcgcc
     3541 gaattcggca tcgacaccgc caatatgttt gaattttggg attgggtcgg cggtcggtac
     3601 agcctgtggt ccgccatcgg attgccgatt atgctgtatc tcggcgaaga aaacttcatt
     3661 gaaatgctca acggcgcgca cctgatggac caacacttca tcaacacacc gctcgagcgc
     3721 aacctgcccg tcattctcgc cctcatcggc atctggtata tcaactacta cggcggcggc
     3781 agccacgtca tcgcgcctta cgaccaacat ttgcaccgcc tgcccaaatt catccagcag
     3841 ctcgatatgg aaagtaacgg caaacaggtt acgttggacg gcaaagcagt cggacacgaa
     3901 acctcgccga ttatctgggg cgaaacgggc attaacggcc agcacgcctt cttccaactg
     3961 ctgcaccaag gcacgcacat tacccccatc gacctgattg cctcgcttga aaaacgcagc
     4021 aacctgcccg gacaccacga aatcctgctt gccaacgtct tcgcccaagc agaagccttt
     4081 atgcgcggca aaacccccga cgaagtccgc gccgaactca aagcgcaggg tatggatgag
     4141 gcgcgcatcg aagagctggt cccgcacaaa accttctccg gtaaccgccc gaccaacctc
     4201 atcctgatgg acaaggtcaa cccgcgtaat atgggcagcc tgattgccat gtacgaacac
     4261 aaaaccttcg tacaaggcat catttggggc atcaacagct tcgaccagtg gggcgtggaa
     4321 ctcggcaaac aactggctaa aaccattttg ggcgaactga ccggcgaaac cgggtcgcaa
     4381 aagcacgaca gctcgaccga acgcctgatc aacctctacc tgcagaccaa ccgcaaataa
     4441 aacctgcgga aaaatgccgt ctgaacgccg accgtttcaa acggcatttt tatcgaacag
     4501 gaaaaccgtc ggtaaacttg cagagcgtgt gcaatcccga tatgatggtt tgcataaatt
     4561 taaacatata tgttccgcag ctatggcact gattaaagag ccgttggaca aagcgaaaca
     4621 aaggaacgaa gaacttgaag cggcagaaga agcggcggcg caggaggcat tgggtcggga
     4681 gcaggaagtc gaccgcgtat ccgaatggga agaacgctac aagctgtcgc gcagcgagtt
     4741 cgagcagctc tggaaaggat tgcctcaaac cgtacagaat aagctgcaag cctcacagaa
     4801 aacatggaaa agcggtatgg acaaaatctg tgccaacaat gcgaaagccg aaggtgaaac
     4861 gccaaacggc ataaaagtca gcgaactggc atgcaaaacg gtggaaaccg aagcacgctt
     4921 ggaagagctg tacaaccgta aaaaagccct tatcgacgaa atggtcaggg aagcggacaa
     4981 gaaagaactg ccaaagcggc tctgaacagc gcggttcagg cactgcccgc cgatattgcc
     5041 gaaaccgtca tgcccgagta ccgcaactga caaaacggtt tgaatgccaa atgcgccgac
     5101 ggcgacgaat acggcgtggc acaatctgac tgccgtacca gagaaatcaa tgcgaaaacc
     5161 aaagaaatcc aaggttatct gattgactga aacttggatg cgggaatgtc ggcggctttt
     5221 gcgtttttgt cgtttttata gtgaattaaa tttaaaccga taca
//
LOCUS       DAXHTR010000139         5260 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.139, whole genome shotgun sequence.
ACCESSION   DAXHTR010000139 DAXHTR010000000
VERSION     DAXHTR010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.139"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(417..1187)
                     /gene="xth"
                     /locus_tag="ACFNML_001740"
     CDS             complement(417..1187)
                     /gene="xth"
                     /locus_tag="ACFNML_001740"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFC6393079.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRSVPQDVHFGLPALPDDPQRRVIAATVSGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRHGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(1247..1528)
                     /locus_tag="ACFNML_001741"
     CDS             complement(1247..1528)
                     /locus_tag="ACFNML_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFC6393080.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSNHLNRLRVEGLVDFTRYHRIIEYRLVSEEAAAILHTVRDLENKRVA"
     gene            complement(1934..2815)
                     /gene="nadC"
                     /locus_tag="ACFNML_001742"
     CDS             complement(1934..2815)
                     /gene="nadC"
                     /locus_tag="ACFNML_001742"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFC6393081.1"
                     /translation="MPSENTLFSLPDTLLRPIVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQAVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTIPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVQQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTIYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFVA"
     gene            complement(2891..3829)
                     /locus_tag="ACFNML_001743"
     CDS             complement(2891..3829)
                     /locus_tag="ACFNML_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393082.1"
                     /translation="MDAYPEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            4136..5248
                     /gene="nadA"
                     /locus_tag="ACFNML_001744"
     CDS             4136..5248
                     /gene="nadA"
                     /locus_tag="ACFNML_001744"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFC6393083.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETAGRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEASTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPEAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSHPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGHNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
BASE COUNT         1163 a         1386 c         1489 g         1222 t
ORIGIN      
        1 ttattgcctt tgggagaatc aattctgaca gcacataaca taggttcatc tttacagtcg
       61 ttatattggg tttcatcaaa ctcaactgca tagcacaata aaggcaatag gaaaaaaagg
      121 agattaattt attactatac ataaggcagc ctgactattc attctaaaat aaatccggtt
      181 tttaatattt gattctgctc cggattatat acataataaa ttttactgct ccaaaatctg
      241 atattaaccc tatggatttc ataaattttt cagaaaaata ggctgtgctt gaacaatccg
      301 ccgactccgg ctgtttgtca gggaaaaggc aattattttc ggttagtcag cagcctatcc
      361 tattgaatca cttaaagata tggatgtttg tcaacgccat atcaaaacac ggtcttttac
      421 caatcgaatt ctgccgtcac cggcgcgtgg tcgctcggac gctccagcgc gcgcgtctcc
      481 aaatcgacgc ggacatcctt caacgccgcc gccatcgcag gcgacaccaa aatatggtcg
      541 atacgcaggc ccagtttgcg ttggaacatc gcgccgcgat agtcgaacca ggtatagaac
      601 gcgccttcgg gatggacttg gcgcaggctg tcggtcagtc ccaaatccag caggttttga
      661 aaccactgcc gttcgacgga cgaacagtgg attttttcgt gccatttttc agggtcgtaa
      721 cagtccgcat cggcaggcgc gatattgaaa tcgcccagca acaccagttt gccgtggcgg
      781 gtcatttcat cgcggacaaa ctccgtcagt gcggcaaacc actgttcctt atatttgaat
      841 ttggggctgt ccaaagcctc gccgttgacg caatagacat tgatgacgcg cacgccgctg
      901 acggttgccg caatcacgcg ccgttgcgga tcgtccggca gtgcgggcaa accgaaatgc
      961 acgtcctgcg gcacgctgcg gctgacgatt gccacgccgt tgtaggtttt ctgcccgctc
     1021 caaacacagt gccagcccat catttgcaaa gcggcggccg gaaatttgtc ctgatcgagt
     1081 ttgagttcct gcaaaaccaa aatatcgggc ggattgtcgg caagcaggtt ttgcacctgc
     1141 ggcagccgca cattgagcga attgacattc caagtggtga ttttcataac atttccgaaa
     1201 aatgctgtct gaaacgttca gacggcaaaa ggaaaggatt ctaacactat gccacgcgtt
     1261 tgttttccaa atcgcgaacc gtgtgcagaa tcgccgccgc ttcttcggaa accaggcggt
     1321 attcgataat gcggtggtaa cgcgtaaaat cgactagacc ttccacgcgc aggcggttca
     1381 aatggttgga aactgcggtg gccggcaggg ataaggattt tgccagttcg gcgatattgc
     1441 gttcgctgtc caacaattga atcagtatcg ccatacgttc gggatgcgcc ataagtttga
     1501 gtatggtgtg gagcggtatt gtgttcatcg gtattcctaa aacctttgcc gcccgccaag
     1561 cggattgacg ggcggcagat atggatgatt gtgattatct ttataaatcg tattataaat
     1621 caaatattta tggatgattg tacaggattg tatgttcctt ttcccacttt tttgcaaaca
     1681 agcgtaaagg cgggctttca gacggcataa aactgccgcc ccccgtcctt tttcctgccc
     1741 taccgatgcc gccgcgctgt cgggcaaaca aaatacctgc ctgaaatgct gttttttcta
     1801 aagcatcacg acctttacga aaaatgccgt ctgaagaatc tttcagacgg catttcctcc
     1861 ctgaggcaat aaccggatta gggttaagcg gttccttgcc tgacatatca gacagccgcc
     1921 cgcaccctaa aactcacgcc acgaaatcca aacctatgtc caacgaacgg ctgctgtggg
     1981 tcagatagcc gagggcgatg ccgtccactc cggtttgcgc cacgcgcttc aggcggtcga
     2041 agccgatgcc gcccgatgct tcgcaataga tggtgtgggg gtgggcggtt tgcgtgtggc
     2101 agcggtttgc cgcttctttc agggtttcgt cgtccatgtt atccagcaaa atccgttccg
     2161 cgcccgctgc gatggcttcg tccagttgtg ccaacgtatc cacttcgatt tccacgcagg
     2221 tcaatgctcc gacagcctgt tttgcctgct gcacggcttg ggcgatgctg ccgcaatagg
     2281 cgaggtggtt gtctttgatg agcacggcgt cgtccaaacc catgcggtgg ttcacaccgc
     2341 cgcctgccct gacggcgtat ttttgcagga cacgcagcag ggggatggtt ttgcggctgc
     2401 acacgatgtc tgtaccgtat tcggcgactt cggcaacggc acgcgcggtg gcggtggcga
     2461 tgccgcttaa gtgcgtgagg tagttgagcg cggtgcgttc ggcggcgagc agcgcgcggg
     2521 cgttgccttc gacggcggca agcgtctgac ctgcgcggac ggcttgcccg tctcggattt
     2581 cggcttggaa gcggacggac ggatccatcg tctgaaaggc gaggcgcgct aaatccatgc
     2641 cggcgataac gccgtcttcg cggctgacga ggaagagttt ggcggttttg tcgggggcga
     2701 taacggcggc ggacgtaatg tcgccgcgcc tgcccaagtc ttcgctcaag gcttgttcga
     2761 ctatggggcg caacagggtg tcgggcaggg aaaagagggt gttttcagac ggcatgaggt
     2821 ttcctttgtc gaaaaagatt gatgttgcac cgatggggca aaacttcatt tgatgccgtc
     2881 tgaaaacggg ctaacggctg cccagcggca atccgatgtc cgaaatcagc ctgtcgggca
     2941 ggacgtcgtc gcggaagcgg caaagctgcg cgggacggcc tttgctgccc gatacgccgg
     3001 tatccgacgg ctcgataagg ttttgctgct gaatctggcg gcggaagttt tgcttgtgca
     3061 gcaatcttcc gctgatggct tcgacactgt tttgcagttg cagcagcgtg aattcgggcg
     3121 gcatcagttc aaaaatcacg gggcggtatt tgattttggc gcgcaggcgg gacagggcgg
     3181 tcgccagtac gcgacggtga tcgtggcgca tgggctgccc cgtgagcgcg aagtcgaagt
     3241 ttgcctgcgg ctcggcggct tccactatca gaccgctttc atacagcatt tcatagcgtt
     3301 gtaaaacgta ttcttccgac cagttttccg gttcgacccc ccagcacaaa tgaatgcgct
     3361 tgagccgcat ttgacgcact tcctccgtgt ccgccgaatt tgcccaaatg cgcaggcggc
     3421 tgacgacggc gtcgcgctgc ccgccgtcgg tgcgcaagtc ttcccacggg aaatagccgt
     3481 agcagtcctg ccatttcgca tccgggtgca ggatgctgtc ggctgcctcg cgcaccagcc
     3541 ccaaatagct gacgtacaag acgggcatgc cgtgttcgtt gcggcggtgg gtatcgacaa
     3601 aggtgtaaag ctgttccaca tagcccatag gctgcgaagt ctgcttggca acccacagct
     3661 tcacgcccgc ctgcagggaa ttgcgcagag gggagagcgg gccgttgggc aggagtgtgc
     3721 cttgggcgac ggttaatacc cgcaggccgc cgtcggtaac ggcaatcaat acgggaacca
     3781 gctcgacgat gctttgcggc ggggcttcgg cttcggggta ggcatccatg ctcggctttc
     3841 tgcgggtggg aaaggcttta ttgtatagtg gattaaattt aaaccagtac agcgttgcct
     3901 cgccttgccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
     3961 tccactataa accaaagcgg tttgtcggaa cgaagcgggt cggttttacg gtatcgcaaa
     4021 cctgttttca gacgaccttg cttattatac tcaatttgag cataatataa aaatatttca
     4081 aaatataaac ggcaaatctt tatgccgtct gaaaaaccag ccataaggaa gtctcatgca
     4141 aaccgccgcc cgccgctcgt tcgattacga tatgcccctc atccaaaccc cgacttccgc
     4201 ctgccaaatc cgtcaggcgt gggcgaaggt tgccgacacg cccgaccgcg agacggcagg
     4261 ccgtctgaaa gacgaaatca aggctttgct gaaggagaaa aacgcggtct tggtggcgca
     4321 ttattacgtc gatccgctga ttcaggattt ggctttagaa acgggcggat gcgtgggcga
     4381 ttcgctggaa atggcgcgct tcggcgcgga acacgaagct agtacattgg tggtggcggg
     4441 tgtgcgcttc atgggcgaga gcgcgaaaat cctctgccct gaaaaaacag tgctgatgcc
     4501 tgatttggag gcggaatgtt ctttggattt gggctgtccg gaagaagcgt tttcagcgtt
     4561 ttgcgaccaa caccccgacc gcacggtcgt agtgtacgcc aacacttccg ccgccgtgaa
     4621 agcgcgtgcc gattgggtgg taacgtcttc ggtggcgttg gaaatcgtat cgtatttgaa
     4681 atcgcgcggc gagaagctga tttggggacc tgaccgccac ctcggcgact acatccgccg
     4741 cgaaacgggc gcggatatgc tgctgtggca gggttcgtgc atcgtccaca acgaattcaa
     4801 agggcaggag ctggcggcgt tgaaggcgga acaccccgaa gcggtggtgc tggttcatcc
     4861 cgaatcgcca caaagcgtca tcgaactggg cgacgtggtc ggctcgacca gcaaactgct
     4921 caaagccgcc gtatcgcacc ctgaaaaaaa attcatcgta gcgaccgact tgggcatcct
     4981 gcacgaaatg caaaagcagg cacccgacaa agaatttatc gccgcaccga cggcgggcaa
     5041 cggtggaagc tgtaaaagct gcgcgttctg cccttggatg gcgatgaatt cgctgggcgg
     5101 catcaaatac gccctgacaa gcggacacaa cgaaatcctg ttggacagga agctgggcga
     5161 agccgccaaa ctgcctttgc agcgtatgct cgacttcgcg gcaggactca agcgcgggga
     5221 tgtgttcaac ggcatgggac ccgcctgatt tgccgtccat
//
LOCUS       DAXHTR010000140         5100 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.140, whole genome shotgun sequence.
ACCESSION   DAXHTR010000140 DAXHTR010000000
VERSION     DAXHTR010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5100
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.140"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            116..637
                     /locus_tag="ACFNML_001745"
     CDS             116..637
                     /locus_tag="ACFNML_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
                     /protein_id="HFC6393084.1"
                     /translation="MSCFSIKRMSAFRARITAFFAAFVFLTAALPAYAERLPDFLAKI
                     QPSEIFPGADRYGKPEGKPMVARVYKGDEQLGLVYITTDVVNTRGYSSKPIDTLMALA
                     KDGTIAGAKLVDHHESIMLIGIPHLPAPGRAIRSNWLPAYIKPNFTSTNRLRLKGLPT
                     VLQPSKETGAGVP"
     gene            526..1725
                     /locus_tag="ACFNML_001746"
     CDS             526..1725
                     /locus_tag="ACFNML_001746"
                     /inference="COORDINATES: protein motif:HMM:TIGR04247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
                     /protein_id="HFC6393085.1"
                     /translation="MASGVYQTKLHIDKPITIEGPADRSATIEGDRSGRTIAVHAPDV
                     TLRNLTVTRSGMSLPAMDAGIYLEETAPRALIEHNNILDNSVGVYLHGSADAMVRENK
                     IVGDATLRVNERGNGVTVWNAPGAQVVGNDISKGRDGIFSNTSTHNTYKNNRFSDLRF
                     AVHYMYTNDSEISGNISVGNNMGYVLMFSERLKVFDNIAVGSRDQGIMLNYVNYSDIH
                     DNIINKAGKCVFAYNANYDKLSANHFENCQIGIHFTAAIEGTSLHDNSFINNESQVKY
                     VSTRFLDWSEGGHGNYWSDNSAFDLNGDGFGDSAYRPNGIIDQIIWRAPVSRLLMNSP
                     AISIVKWAQAQFPAVLPGGVVDSKPLMKPYAPKIQTRYQAMKDGLLKEAETRQLEWGR
                     AENGSLN"
     gene            1783..2397
                     /locus_tag="ACFNML_001747"
                     /pseudo
     CDS             1783..2397
                     /locus_tag="ACFNML_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            2492..2623
                     /locus_tag="ACFNML_001748"
                     /pseudo
     CDS             2492..2623
                     /locus_tag="ACFNML_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688339.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            2620..3114
                     /locus_tag="ACFNML_001749"
     CDS             2620..3114
                     /locus_tag="ACFNML_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HFC6393086.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(3202..4863)
                     /locus_tag="ACFNML_001750"
     CDS             complement(3202..4863)
                     /locus_tag="ACFNML_001750"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HFC6393087.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSETGAHSLAGAVIDPIALNELIPDWKEKGAPLTRTVTQDKVLFLTKKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GVGKDGEPTDSFQPGMELWARQTLFAEGCRGSLSKQIIERFKLDQNSQPQTYGLGMKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGVLIGDAAGFLNMP
                     RIKGIHTAMKSAMIAAEAVFPLLEHLEEAEGFDSGKEAADYQQRFEQSWLYRELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHYGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
BASE COUNT         1186 a         1368 c         1333 g         1213 t
ORIGIN      
        1 tatcccgtat gcggattggt tttaagattt gtaaatttga tttgcatcaa aaaatcgccg
       61 atagatgatt catataatat caatattaaa gagtatcggt atatcgggga tagtcatgtc
      121 ctgtttttca atcaaacgta tgtccgcgtt tcgggcgcgg ataacggcgt tttttgccgc
      181 ctttgtcttt ttgacggcgg cactgcccgc ttatgcggag cgtctgcctg attttctggc
      241 gaaaatacag ccttcggaaa tttttccggg tgcggaccgt tacggcaagc cggaaggtaa
      301 gcctatggtt gcccgcgttt acaaaggcga tgagcagctc ggtttggtct atatcacaac
      361 cgatgtggtc aatacgcgcg gttattcgag caaaccgatt gatacgctga tggcgttggc
      421 taaagacggt acgatagccg gagcgaaatt ggttgatcac catgagtcga ttatgctgat
      481 cggtatcccg catttgcccg cgcccgggcg ggcgatacgc tcaaactggc ttccggcgta
      541 tatcaaacca aacttcacat cgacaaaccg attacgattg aagggcctgc cgaccgttct
      601 gcaaccatcg aaggagacag gagcgggcgt accatagccg tacacgcgcc ggacgtaacg
      661 ctccgcaacc tgaccgttac ccgttccggt atgagcctgc ccgcaatgga tgccggtatt
      721 tatctcgaag aaactgcccc gcgcgccctg attgaacaca ataatatttt ggataattcg
      781 gtcggcgtct atctgcatgg ttctgccgat gcgatggtgc gggagaataa aatcgtcggc
      841 gacgcgactt tgcgcgtgaa cgagcgcggc aatggcgtta ccgtttggaa cgcgcccggc
      901 gcgcaggtcg tcggcaacga tatttccaaa gggcgggacg gcattttttc caataccagc
      961 acgcacaaca cctataaaaa caaccgcttc agcgatttgc gtttcgccgt ccactatatg
     1021 tacaccaacg acagcgaaat cagcggcaat atttccgtgg gcaacaatat gggctatgtg
     1081 ctgatgtttt ccgagcggct caaagtgttt gacaatatcg ccgtcggcag ccgcgaccaa
     1141 ggcatcatgc tcaactatgt caactattcc gatattcacg acaacattat caacaaagcg
     1201 ggcaagtgcg tttttgccta caatgccaac tacgataaac tgtccgccaa tcattttgaa
     1261 aactgccaaa tcggcataca ctttaccgcc gccatcgaag gcacgtcact gcacgacaat
     1321 tcctttatca acaacgaaag ccaggtcaaa tacgtcagca cgcgctttct cgactggagc
     1381 gagggcggac acggcaacta ttggagcgac aacagcgcgt tcgatttgaa cggcgacggc
     1441 ttcggagaca gcgcgtaccg tcccaacggc atcatcgacc aaatcatctg gcgcgcaccc
     1501 gtatcgcgcc tcttgatgaa cagtcccgca atcagcatcg tcaaatgggc gcaggcgcag
     1561 tttcccgccg ttctgcctgg cggcgtggtg gacagcaaac cgctgatgaa gccttatgcc
     1621 cccaaaattc aaacccgtta tcaggcgatg aaggacgggc tgctcaaaga agccgaaacg
     1681 cggcagttgg aatggggcag ggcggaaaac ggttctttga actagtctgt ttcagacggc
     1741 atccggattc aaatgccgtc tgaaaacaca aaaggaaaaa ccatgaccac acatcatgtc
     1801 gaattgagga atgtaaccaa acggttcggg gcgcaaaaag cggtcaacca agttgatttg
     1861 gttttgaagg caggagaaag cgtcgggctt gccggacaca acggcgcggg caagtccacc
     1921 attatgaagc tgatactcgg gctgattacc ccgaccgaag gcgaagtgat gcttttgggc
     1981 gaacgtaccg gtagcaaagc gggggcgcgg cttcgcagcc aaatcggcta cctgcccgaa
     2041 accgttgcgc tgcccccttc gctgaccggc atcgaaacgc tggattttta tgccaaactt
     2101 aaaaaacagc cgctcacgca gaaccggggg ctgcttgagc gtgtcggtat ttcacaggcg
     2161 gcacaccgcc gcgtcggcac ttattctaaa gggatgcgcc aacgccttgc cttggcacaa
     2221 gccctgctgg gcgagcccaa agtcctgctg tttgacgaac cgacaaccgg cctcgaccct
     2281 gcatcacgac aaatgtttta cgaagtcgtg cgcgaactca acgggcgcgg cgcgaccgta
     2341 ttgctcagca cccacgccct tgccgagttg gacggacacg ccgaccgcat tatcgtggat
     2401 taaatttaaa ccagtacggc gttacctcgc cttgccgtac tatttgtact gtctgcggct
     2461 tcgttgcctt gtcctgattt ttgttaatcc actatatgcg ggtatggcgg gtttgagcgg
     2521 acaaatcagc ctgaccgtcc ccgttttgct gaccgctcag gttttatggg ttatcattcc
     2581 gcttgttttg gcagccggaa tttttagaaa gcgacaaata tgaaaaaaac cctgttggca
     2641 attgttgccg tttccgcctt aagtgcctgc cggcaggcgg aagagggacc gccgccttta
     2701 ccccggcaga ttagcgaccg ttcggtcgga cactattgca gtatgaacct gaccgaacac
     2761 aacggcccca aagcccagat tttcttgaac ggcaaacccg atcagcccgt ttggttctcc
     2821 accatcaagc agatgttcgg ctataccaag ctgcccgaag agcctaaagg catccgcgtg
     2881 atttacgtta ccgatatggg caatgttacc gattggacga atcccaatgc cgacacggag
     2941 tggatggatg cgaaaaaagc cttttacgtc atcgacagcg gctttatcgg cggtatgggt
     3001 gcggaagacg cgctgccgtt cggcaacaag gaacaggctg aaaaatttgc aaaggataaa
     3061 ggcggtaagg ttgtcggttt cgacgatatg cctgatacct atattttcaa ataatattga
     3121 aaaaaccggc cgaaacttta aaaacgagtt tcggtcggtt tttcttttct tgttcggtat
     3181 agtgtcggca ggaaagaacc ttcacatccc gccgtaattc ggcccgctcg cgccttcggg
     3241 gcaaatccaa gtgatgtttt gcgtcgggtc tttgatgtcg caggttttgc agtgcacgca
     3301 gtttgccgcg ttgatttgca ggcgcggatt gccgttttct tcaacaattt cgtacacgcc
     3361 ggccggacaa tagcgcgttt cgggcgaggc gtattctttg tagttcacgt ctatcatcgt
     3421 ttgcggattg ttcagcacca aatggtcggg ctggttttct tcgtgcgcga gattggcaag
     3481 gaagacgctg ctcaagcggt cgaaggtcaa cacgccgtcg ggtttcggat aatcaatcgg
     3541 tttgcacgcg gcggcttttt tgagctgctc gttgtctttg ccgtaatgtt tcaaggtcca
     3601 cggggctttg cctctgaaaa tcatctggtc gatgccggta tagattgagc cgaggtaaac
     3661 gccccatttg aatgacggac ggacattgcg cgcggcgtaa agctctcgat acagccagct
     3721 ttgttcaaaa cgttgctgat aatccgccgc ttctttgccg ctgtcgaaac cctccgcttc
     3781 ttcaaggtgt tccaacaagg ggaacacggc ttcggcggcg atcatagcgg atttcatcgc
     3841 ggtatggatg cctttgatgc gcggcatatt gaggaaacct gccgcatcgc cgattaaaac
     3901 gccgcctttg aacgagagct tcggcaaact ttgcaaaccg ccttcaatca gcgaacgcgc
     3961 gccgtaagca atgcggcggc cgccttcaaa ggttttgcgg atttcgggat gggttttgaa
     4021 acgttggaac tcttcaaacg gcgacagata aggattttga tagtccaaac cgaccacgaa
     4081 gccgacggcg actttgttgt cgtcgaaatg gtaaacaaac gcgccgccgt aggttttgct
     4141 gtccagcggc cagcctgcgc tgtgcaccac caaaccgggc tgatgctgtt cggacggcac
     4201 ttcccaaact tctttcatgc ccaagccgta agtttgcggc tggctgtttt ggtcgagttt
     4261 gaagcgttcg atgatttgtt tggaaagcga acctcggcag ccctcggcaa acagggtttg
     4321 ccgtgcccaa agctccatgc cgggctggaa tgaatcggtc ggctcgccgt ctttgcccac
     4381 gcccatattg ccggtcgcaa tgcctttgac cgaaccgtct tcgtgataca gtacttcggc
     4441 agcggcaaag ccaggataga tttccacgcc catattttcc gcctgctccg ccaaccagcg
     4501 cacgacttcg cccaagctga cgatgtagtt gccgtgattg tcgaaattcg gggtaatagg
     4561 cagattgaac gcctttttct tagtcaggaa caatactttg tcctgcgtta ccgtacgcgt
     4621 cagcggcgcg cctttttctt tccaatcggg aatcagctcg ttcagcgcaa tcggatcgat
     4681 aaccgcgccc gccagcgaat gcgcgcccgt ctcagaacct ttctccacca cgcaaacgct
     4741 gatttcgcgc ccgttttgtt cggcaagctg cttgagtttg atggcggcag acaaacccga
     4801 cgggcctgcg ccgacaatca cgacatcgta ttgcatactg tcgcgggtga tggattctgt
     4861 catggcggtt cctgtgtatt tattattgaa ttgcaaatcc gtaattatac aacgggaaca
     4921 tatagttacc aaatacaaca aaggtcgtct gaaaaccata ttttcggttt tcagacgacc
     4981 tttgtcgaaa tttcaataag cacgccacca ttttacctgt ccgaccgcaa actccgtctg
     5041 acgtttcgga ctgcgtgtga aaaacgcctt atccccgccg gcatccctcc ctttcggcac
//
LOCUS       DAXHTR010000141         4906 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.141, whole genome shotgun sequence.
ACCESSION   DAXHTR010000141 DAXHTR010000000
VERSION     DAXHTR010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4906
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.141"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(458..802)
                     /locus_tag="ACFNML_001751"
     CDS             complement(458..802)
                     /locus_tag="ACFNML_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393088.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(840..1163)
                     /locus_tag="ACFNML_001752"
     CDS             complement(840..1163)
                     /locus_tag="ACFNML_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393089.1"
                     /translation="MFLDDVNVFLDDLNTNPITDEWYMSNFADKHIKILESYETFDIL
                     KQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQEISQDIL
                     NEIFR"
     gene            complement(1166..1450)
                     /locus_tag="ACFNML_001753"
     CDS             complement(1166..1450)
                     /locus_tag="ACFNML_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393090.1"
                     /translation="MAGGNKSLKIGTQSVEKSTGRTIPNNLKEQLAMEEVKANPQGKT
                     PARIPPMSDTKNGWLAKDGWVKRVQNVNKIEIHYIENSRTGEKTDFKFKD"
     gene            complement(1433..1759)
                     /locus_tag="ACFNML_001754"
                     /pseudo
     CDS             complement(1433..1759)
                     /locus_tag="ACFNML_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(1865..2236)
                     /locus_tag="ACFNML_001755"
     CDS             complement(1865..2236)
                     /locus_tag="ACFNML_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HFC6393091.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(2233..2588)
                     /locus_tag="ACFNML_001756"
                     /pseudo
     CDS             complement(2233..2588)
                     /locus_tag="ACFNML_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504007.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2616..2822)
                     /locus_tag="ACFNML_001757"
     CDS             complement(2616..2822)
                     /locus_tag="ACFNML_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393092.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(2832..3677)
                     /gene="mafB"
                     /locus_tag="ACFNML_001758"
                     /pseudo
     CDS             complement(2832..3677)
                     /gene="mafB"
                     /locus_tag="ACFNML_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(3774..3986)
                     /locus_tag="ACFNML_001759"
     CDS             complement(3774..3986)
                     /locus_tag="ACFNML_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393093.1"
                     /translation="MKLINFEESSVKISLDKDELYIIQAIVGEIYSGVCVDCRDFEII
                     HGVEKIKFFLWIKNLKKYMILGINVS"
     gene            complement(4063..4422)
                     /locus_tag="ACFNML_001760"
     CDS             complement(4063..4422)
                     /locus_tag="ACFNML_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HFC6393094.1"
                     /translation="MRLKLKNYDCSDFDLEDFPQDKLENFCILLTLSIGFDESNGADY
                     FYVYIYSTEWLLSNIHRPMSLKNSIVTNRFNIEHILKLINDILEICNSTSEDKSISNL
                     AKYFDWEFDDYNLNIQD"
     gene            complement(4427..>4906)
                     /locus_tag="ACFNML_001761"
     CDS             complement(4427..>4906)
                     /locus_tag="ACFNML_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC6393095.1"
                     /translation="KWDWVKNTDYKTPAARPMQTLDGEMAGGNKPPKSITSGGKANAA
                     TYPQLVNQLTGQNLKNIAAQDPRLSLALHKSEKNFLIGTATYEEADRLGKIWVGEGAR
                     QTSGGGWLSIDGTRQYRPPTEKNSQFATTGIQANFETYTIDSNGKRNKIKNGHLNIR"
BASE COUNT         1361 a          960 c          776 g         1809 t
ORIGIN      
        1 tcattaatac cttgtaaagt ttgctgcgca gagatgtagg tcggatattt gtatccgaca
       61 aaaatatttg acgcatctat cgtttccgaa atgccgctgt tggaaaatgt cggattcgag
      121 aatccgacct acggctattt cgtttaagtc ataaatagcc attcatgttc cttctgaatt
      181 tgcttttgct atccttagaa ccaatcatat aaacattatc atcattaatt aatgttctaa
      241 gtttatgaag cggtcaaatc tgtcaaacta ccagagatga agcatatcct cctggaagta
      301 attctgttcg tgctgtttcc atatcttctt tgccatcatc agttacttct ctgtctaaat
      361 aaaatcttac gataagtttt tgtctaattt catcaaattc tgccgtaatt aatcttatat
      421 tgatatgaat acacggttca tacctgctat tagccagtca ggtgtagttg gatggggatt
      481 catttttaat aatatcttca agcaagtgaa atgtaagaaa agcgtagata cttgaaggcc
      541 ggctatctga ttggcaaaaa gaaatttcgg agataaagaa tttccgttta tatttttcat
      601 aaattaaatc aacaccttgt tttacataaa aactaacttc ctcaggaagg atatttataa
      661 cattctctcc ttttactttt atagttaatg gtttgatttt tatgttttca tgaatatctg
      721 ataggattat ttctaaaaaa ttatttttat ttccagtcat tctggctatt ttatgtatat
      781 ttccattgtg ttcgattatc attttatttt ttccaaggat ttccttctat attgatagtt
      841 tatctaaaga tttcatttaa aatatcctga ctaatttctt gtttatataa ttctacaatt
      901 ttctgtttct gtgtatttgt ataaaatttt tcgttggtat ctgcttgata ttttaattgt
      961 ctcaatattt ccatgatttc atattctgat ttttcatcat attcttcaat catgtaatca
     1021 acaaattgtt ttagaatatc aaaggtttcg taactttcca aaattttaat atgtttatcg
     1081 gcaaaattgg acatatacca ttcgtcagtg attggattag tatttaaatc atctaaaaaa
     1141 acatttacat catctaaaaa catgactaat ccttaaactt aaaatctgtt ttctcaccgg
     1201 ttcttgagtt ttcaatgtaa tgtatttcaa ttttgtttac gttttgaaca cgcttaaccc
     1261 aaccgtcttt tgctaaccaa ccatttttag tatcggacat aggaggtatt ctcgcaggag
     1321 ttttgccctg tgggtttgcc ttaacttctt ccattgctaa ttgttccttt aaattattag
     1381 gtattgtacg accggttgat ttttcaacag attgtgtccc tatttttaat gatttgtttc
     1441 ccccagccat ttccccgtct acagtctgca tagggcgggc agcaggtttt ttatagccgg
     1501 tatttttaac ccaatcccat ttagtcgggt taagttctac ttttttaccg cgccaaaccg
     1561 tacctgcggc ctctgctacg gaaagggcag tttgggctgt tgcagttata ttcggatggg
     1621 catcagccca ttgtctggcg gaattaatgc cgttttttac cgcaaaagca ctgtcctgta
     1681 ataggctcgc ggcagcaagt tgtgcttccg gacttaaatt tcctaattca ttaatatctt
     1741 gtaaagtttg ctgcgcagag atgtaggtcg gatatttgta tccgacaaaa atatttgacg
     1801 cgtctatcgt ttccgaaatg ccgctgttgg aaaatgtcgg attcgagaat ccgacctacg
     1861 gctgctacca accatcattt tcagcttctt ttagccaagt atcaaaattt tcaaaagtct
     1921 cgtaatattt tgtattatct aaattaacta ctataatttt aggattacct gatttatctg
     1981 aaatatcaaa acaggcagcc cactcttggt ttctagcaaa aggtaatagg tttggaatgc
     2041 cagttatttc aaaataaatg tcaattactt ctgatatatt gctttctgca ttttcaaacc
     2101 accaaggaaa agaatattgt tcatcgtagt taatggcatg agtggattga gctaatttta
     2161 aataactttg cggatattta aatcctttag gaagcattgc ttcaggatac ggaacaaaac
     2221 tattttgttt tatcatttcc taagtacctt tacatcataa atacttccgt taggaaatct
     2281 tttgctccac ccttctggaa ctcctttttg tgtttcttgc tttctaattc gtagctggat
     2341 agcttctccc caagtttgag gctcaatacc atcatgctta aacctgattc tctcttttga
     2401 tgataatcgg tcattaaaat tatgaacatt cgcttccact tttacccctg cttcatgtgc
     2461 tgccataata cgcgtgttat ccatactagt aggtgctcca tcaggcatat taacaacatc
     2521 aaccctgtcg ccaacccatg atttttttcg ttcattgcag ctacaagaga atcatagttg
     2581 taatttgcac taggctctga caaaaatttg ggttgttaag ttgtcatttt tttataaatt
     2641 ccagcaaact gttgttgtaa attatctact tcatgttttc taacaccaga tacattttca
     2701 aattctcttg aatctacaca tacgcccgca tatatctcat tcaagatagc tcgtaaaaca
     2761 aaaacttcat cttgagttag tgaaatagtt gagttttgat tatttatgga tagtatattc
     2821 atggcttttt cctataaatc atcataatat tttttacctg atgttggtct aaatgctgta
     2881 cctccatcgt cagaattttt atctcggata actattgtcc ctgttttatc atcccaatac
     2941 gcagttctac cgcgaggtaa ctctttcata tttgttggat ggctaacaat attttcaata
     3001 tgccgagcaa aatctgctgg tgaattgata tttaaatccg taaattcttg ttgtcttatg
     3061 acatgcttgt tataagcatg cccactacta atttgttctc caattagttg cgcttgtaaa
     3121 gacggttgtg tggaagcatc tgctttgctg ttggacgtta tagatttagg cggtctattc
     3181 ccccctgcca tctccccatc tacagtctgc atatggcggg cagcaggttt tttatagccg
     3241 gtatttttaa cccaatccca tttggtcggg ttaagttcta cttttttacc gccccaaacc
     3301 gtagttgcgg cctctgctac ggcaagggca gtttgggctg ttgcagttat attcggatgg
     3361 gcatcagccc attgtttggc agagttgata ccgtctttta ccgcaaaagc actgtcttgt
     3421 aatgcggttg cagccgcaag ttgtgcttcg ggacttaaat ttcctaaatg attaatacct
     3481 tgtagagtct gctgcgcggc tgtatctgtg accgggctta ctgcactgtc tgtaattgcc
     3541 ccaatcccta ctccttgaaa acccattaaa aaaggattaa ccgcaccttg gacgatgccg
     3601 cgaacatcat ccatacggtt agcccaccaa tttttattgg ggtcgttttc ccatatcagt
     3661 tttcctgctt catcagcaca gttaggattg gcattgatgc ccacccagtt tcaggcagcc
     3721 tgaaaaggat ttttaatata cggcaaaagg tgggcaacaa gtgcccacct tacctagcta
     3781 acatttatcc caagtatcat atattttttt aagttcttta tccaaagaaa gaactttatt
     3841 ttttcaacac catgaattat ctcaaaatct ctacaatcca cgcatacgcc tgaataaatt
     3901 tctcctacaa ttgcttgaat aatatacaac tcatctttat cgagactaat tttaactgaa
     3961 ctctcttcaa aattaattaa tttcatgaat aattcctttt attttaattc gtcatcaaaa
     4021 taccgtttgc ctgtttttgg tctaaatgct gtcccaccat ctttagtctt ggatatttag
     4081 attataatcg tcaaattccc agtcaaaata ttttgccaaa tttgatatag acttatcttc
     4141 agacgttgaa ttacatattt ccaatatatc atttatcaat tttaatatgt gttcaatatt
     4201 aaaacggttt gtaacaattg agttttttaa tgacatcggt ctatgtatat ttgaaagtaa
     4261 ccattcagtg ctatatatat aaacataaaa ataatcagca ccattacttt catcaaaacc
     4321 tattgataat gtcaaaagta tacaaaaatt ttctaacttg tcttgtggga aatcttccaa
     4381 atcaaagtca gaacaatcat aattttttaa ttttaatctc atataattac ctaatattta
     4441 aatgtccatt tttaatttta ttcctttttc catttgaatc aatagtataa gtttcaaaat
     4501 ttgcttgaat acctgtagtt gcaaattgtg aatttttttc tgttggtggc cgatattgtc
     4561 gagtgccatc tatacttaac catccgcctc cactagtttg tcttgcaccc tcaccaaccc
     4621 aaattttacc tagcctatct gcctcttcat aagttgcagt tcctattaga aaattttttt
     4681 cactcttatg aagagctaga ctcaatcttg gatcttgagc cgcaatgttt tttaagtttt
     4741 gcccagttaa ttgattaact aattgaggat aagttgcagc attggctttt cctccagacg
     4801 ttatagattt aggcggttta ttcccaccgg ccatttcccc atctaaagtc tgcataggtc
     4861 gggcagcagg tgttttataa tcggtatttt taacccaatc ccattt
//
LOCUS       DAXHTR010000142         4773 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.142, whole genome shotgun sequence.
ACCESSION   DAXHTR010000142 DAXHTR010000000
VERSION     DAXHTR010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.142"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(398..1050)
                     /locus_tag="ACFNML_001762"
                     /pseudo
     CDS             complement(398..1050)
                     /locus_tag="ACFNML_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            1657..2466
                     /locus_tag="ACFNML_001763"
     CDS             1657..2466
                     /locus_tag="ACFNML_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RTX iron-regulated FrpC family protein"
                     /protein_id="HFC6393096.1"
                     /translation="MRPYATTIYQLFILFIGSVFTMTSCEPVNEKTDQKAVSAQQAKE
                     QTSFNNPEPMTGFEHTVTFDFQGTKMVIPYGYLARYTQDNATKWLSDTPGQDAYSINL
                     IEISVYYKKTDQGWVLEPYNQQNKAHFIQFLRDGLDSVDDIVIRKDACSLSTTMGERL
                     LTYGVKKMPSAYPEYEAYEDKRHIPENPYFHEFYYIKKGENPAIITHRNYHRYGENDY
                     STSVGSCINGFTVRYYPFIREKQQLTQQELVGYHQQVEQLVQSFVNNPSKK"
     gene            2483..>4773
                     /locus_tag="ACFNML_001764"
     CDS             2483..>4773
                     /locus_tag="ACFNML_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HFC6393097.1"
                     /translation="MNEGEVVLTPEQIQTLRGYAFRGDTYGGWRYLANLGDRYADDAA
                     AIVGKDANLNGLNLWMKKGVENLWDDTVGKKTRLEKFDRVALQHFSQYVDLINENNGR
                     LPNTSEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQA
                     VNNPNGSEWDNRKQLISALDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIG
                     GWGNGIINDLYKSVAKREWTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKET
                     KKRNGKYHVYDPLALDLDGDGIETVATKGFAGSLFDHTNNGIRTATGWVSADDGLLVR
                     DLNGNGIIDNGAELFGDNTKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVW
                     QDLNQDGISQANELRTLEELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTDGTTAKM
                     GDLLLAADNLHSRFKDKVELTAEQAKAANLAGIGRLRDLREAAALSGDLANMLKAYSA
                     AETKEAQLALLDNLIHKWAETDSNWGKKSPMRLSIDWTQTANEGIALTPSQVAQLKKN
                     ALVSLSDKAKAAIDAARDRIAVLDAYTGQDSSTLYYMSEEDALNIVKVTNDTYDHLAK
                     NIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSGLVQAFNHVKETNPQKAFVDLAEML
                     AYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKVLGQETVALLAKTSGTQADDILQNV
                     GFGHNKNVSLYGNDGNDTLIGGAG"
BASE COUNT         1471 a          975 c         1053 g         1274 t
ORIGIN      
        1 cttttccgat aaatgaccgc aacctaaaac ccatccttcc cgcaaaaaca gaaaaacaaa
       61 aacctaaaat cccgtcatcc ccacgataac agttgcgtaa ttgcgtagag tgggcttcag
      121 cccaacgttt tttctttttc ggtcgttgat tggtgggctg aagcccaccc ttgtatatcg
      181 gaactcccgt atcatagcaa caaaccgccc ggccgccacc cgcgcccacc caaggcacac
      241 aaccgttgcg tagcacaggg agcggcaggg caacccatcg acacaaccgg acagttgccg
      301 gacaacacaa ccgaatgcaa ggcaggttga tgatgagtac ccgataccat tacgcaggta
      361 tagtgaatta aatctaaggg gctgtactag attagcccta aattccacac caatcccgca
      421 ggattttaag ctgttgagac ggtgtgccga agttaaatcg aaattcgcat tctttcaaga
      481 acagcgggaa agatttgcaa tcgattccgt tgtattttcg caagacgcgt tttgcctgat
      541 tccaaaagtt ctcaatgccg ttaatgtggt tttgacggtc tgcaaactcc ttggaatggt
      601 tgatgcggta atggataaaa ccgctcacgt ccaacttgtc gcagctgctc agactatcgg
      661 tataaacaat actgtccggc atgattttct ttttgatgac agggagtaac gtttcagact
      721 tggcattgtt tacgacaacg gtatagaccc gtccgttgcg tttcagaatg ccgaagacaa
      781 ccacttttcc tgccgcccgc gaccacgtct gcctttacgc cgtccgccga aatcgctttc
      841 gtccggctcg acagggccct caaaaacctc atcggcagcc aaggccaaat gatggttgat
      901 aaccgtgcgg attttacggt agaacagtgc tgccgaattg ggatggatac ccaaaatatc
      961 ggcggcagaa cgggcggtaa tttccagcgc aaaaaaacgg agcagttctt tctgtacttt
     1021 tttctttaat ttgcagtgtg ttatcttcat atttcgaggg taacatatct gctaatctag
     1081 tacagcccca aaaatatacc aaaaacagca aaacaaattg taaggatagg tataggcttt
     1141 gtaaaggtaa attgtgaaaa aagcagtttt ttaaacgaat gaaacggctt cgggctgaaa
     1201 tatatgctga tgccctgtcc ttcccgtata tcttgtgtgt tgtcaaagtg caggctgctt
     1261 tgaaattggc attacagtcg atgaaccacc attttgcttt atttcagcgg gcttgagatg
     1321 tgtataagaa tattgttttg aataaattta aagaaaatga taatcgttat tgacgatttt
     1381 taaaggaaag cgtagagtgc caattctatg aagcaatacg gtaagtaaca atgaaaatat
     1441 ctactgcttg ggtatagagc atatttcaca acccgtaact attcttgcgg aaacagagaa
     1501 aaaagtttct cttctatctt ggataaatat atttaccctc agtttagtta agtattggaa
     1561 tttataccta agtagtaaaa gttagtaaat tatttttaac taaagagtta gtatctacca
     1621 taatatattc tttaactaat ttctaagctt gaaattatga gaccatatgc tactaccatt
     1681 tatcaacttt ttattttgtt tattgggagt gtttttacta tgacctcatg tgaacctgtg
     1741 aatgaaaaga cagatcaaaa agcagtaagt gcgcaacagg ctaaagaaca aaccagtttc
     1801 aacaatcccg agccaatgac aggatttgaa catacggtta catttgattt tcagggcacc
     1861 aaaatggtta tcccctatgg ctatcttgca cggtatacgc aagacaatgc cacaaaatgg
     1921 ctttctgaca cgcccgggca ggatgcttac tccattaatt tgatagagat tagcgtctat
     1981 tacaaaaaaa ccgaccaagg ctgggttctt gagccataca accagcaaaa caaagcacac
     2041 tttatccaat ttctacgcga cggtttggat agcgtggacg atattgttat ccgaaaagat
     2101 gcgtgtagtt taagcacgac tatgggagaa agattgctta cttacggggt taaaaaaatg
     2161 ccatctgcct atcctgaata tgaagcttat gaagataaaa gacatattcc tgaaaatcca
     2221 tattttcatg aattttacta tattaaaaaa ggagaaaatc cggcgattat tactcatcgg
     2281 aactatcata ggtatggaga gaacgattac agcactagcg taggttcctg tattaacggt
     2341 ttcacggtac ggtattaccc gtttattcgg gaaaagcagc agctcacaca gcaggagttg
     2401 gtaggttatc accaacaagt agagcaattg gtacagagtt ttgtaaacaa tccaagtaaa
     2461 aaataattta aaggatctta ttatgaatga gggtgaagtt gttttaacac cagaacaaat
     2521 ccaaaccttg cgtggttatg ctttccgtgg cgatacctat ggcggttggc gttatttggc
     2581 taatttgggt gaccgttatg cggatgatgc tgctgcaatt gtcggtaagg atgcaaactt
     2641 aaatggtttg aatttatgga tgaaaaaagg tgtggaaaac ctatgggatg atacggtcgg
     2701 taaaaagacc cgtttagaga aatttgatcg ggttgcattg caacatttca gccaatatgt
     2761 agatctaatt aatgaaaata atggtagatt acctaacact agtgaaattg agagaagtta
     2821 ctataaagcc gttaccgaaa atggtgtttc ttctagtgca gctattgatt tagttattaa
     2881 tcgctcactt ccggatatgg cagatggtta ttgggcatta ggtttgggga tagaagccga
     2941 acgtatccac aatgcgcaag cagtaaataa tccgaacggt agcgaatggg ataatagaaa
     3001 gcagttaata tctgctttag ataaaggatt tgatggatct tttaaagaga agcattttac
     3061 ttttttacaa tctgtgataa tggatgtaac aaagttaggt gttgaatata caatagatgg
     3121 ttggcaaaaa attggaggtt ggggtaatgg gataatcaat gatttatata aaagtgttgc
     3181 aaaaagagag tggactggaa tatttgagat cgttaataat aacatcaagc aatttagaga
     3241 tctgttccca aatccggaag gctggatcga tgatggtcac caatgtttcg ctccttgggt
     3301 taaagaaact aaaaaacgca atggcaaata tcatgtctac gaccccctcg ccctagattt
     3361 ggacggagac ggtatagaaa ccgttgccac caaaggcttt gcaggcagct tatttgatca
     3421 caccaacaac ggtatccgca ccgccaccgg ttgggtttct gccgatgacg gtttactcgt
     3481 ccgcgatttg aacggcaacg gcatcatcga caacggtgcg gaactcttcg gcgacaacac
     3541 caaactggca gacggttctt ttgccaaaca cggctacgcg gctttggccg aattggattc
     3601 aaacggcgac aacatcatca acgcggcaga cgccgcattc caaaccctgc gtgtatggca
     3661 ggatctcaat caggacggca tttcccaagc taatgaattg cgtacccttg aagaattggg
     3721 tattcaatct ttggatctcg cctataaaga tgtaaataaa aatctcggta acggtaacac
     3781 tttggctcag caaggcagct ataccaaaac agacggtaca accgcaaaaa tgggggattt
     3841 acttttagca gccgacaatc tgcacagccg cttcaaagac aaagtggaac tcactgccga
     3901 acaggcaaaa gccgccaatc ttgcgggcat tggtcgtctg cgcgatttgc gcgaagctgc
     3961 cgcattgtcc ggcgatttgg ccaatatgct gaaagcttat tctgccgccg aaactaaaga
     4021 agcacagttg gcattgttag ataatttgat tcacaaatgg gcggaaaccg attcgaactg
     4081 gggcaaaaaa tcgccaatgc gactttcaat cgattggacg caaacggcta atgaaggtat
     4141 tgcactgaca ccatcccaag tagcacaact aaaaaagaac gctttagttt ccctttctga
     4201 taaagctaaa gcagctattg acgccgcccg cgaccgcatt gccgtgcttg atgcctacac
     4261 ggggcaggat tccagcacac tctattacat gagcgaagaa gacgcgctta atatcgtcaa
     4321 agtaaccaac gatacatacg accatctcgc caaaaacatc taccaaaacc tgttgttcca
     4381 aacccgtttg cagccatatt tgaatcaaat cagtttcaaa atggaaaatg atacgttcac
     4441 tttggatttt agtggtcttg ttcaagcatt taaccatgtc aaagaaacta atccgcaaaa
     4501 agcttttgtg gatttggccg agatgcttgc atatggcgaa cttcgttctt ggtatgaagg
     4561 ccgaagacta atggccgatt atgtggagga ggcaaaaaaa gcaggtaaat ttgaagatta
     4621 ccagaaagtg ttgggtcagg agaccgttgc attattagct aaaacatcgg gtacgcaagc
     4681 agatgatatc ctgcaaaatg taggctttgg tcataataaa aatgtttctt tatatggtaa
     4741 tgacggcaac gacactctga tcggcggtgc cgg
//
LOCUS       DAXHTR010000143         4684 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.143, whole genome shotgun sequence.
ACCESSION   DAXHTR010000143 DAXHTR010000000
VERSION     DAXHTR010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.143"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            82..891
                     /gene="aroE"
                     /locus_tag="ACFNML_001765"
     CDS             82..891
                     /gene="aroE"
                     /locus_tag="ACFNML_001765"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFC6393098.1"
                     /translation="MHALPRYAVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFETGGCGANVTVPFKQEAFHLADEHSERALAAGAVNTLIFLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTHAKA
                     EELARLFGIEAVPMADVNGGFDIIINGTSGGLSGQLPAVSREIFRNCRLAYDMVYGDA
                     AQAFLNFAQSNGAAEVSDGLGMLVGQAAASYHIWRGFTPDIRPIIEYMKAL"
     gene            894..1595
                     /gene="mtgA"
                     /locus_tag="ACFNML_001766"
     CDS             894..1595
                     /gene="mtgA"
                     /locus_tag="ACFNML_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFC6393099.1"
                     /translation="MFRIIKWLIALPVGIFIFFNAYVYGNIITYRAVAPHQTAFMSMR
                     MKQFEQEGRDVALDYRWMPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLTKQQAAKLTACVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(1920..1995)
                     /locus_tag="ACFNML_001767"
     tRNA            complement(1920..1995)
                     /locus_tag="ACFNML_001767"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1960..1962),aa:Phe,seq:gaa)
     gene            complement(2186..2920)
                     /gene="lptB"
                     /locus_tag="ACFNML_001768"
     CDS             complement(2186..2920)
                     /gene="lptB"
                     /locus_tag="ACFNML_001768"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFC6393100.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVTLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEIRTKDKNQIDREIEKLLADLNIGHLRRSPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGAV
                     LASGKPDDLVGNEQVRSVYLGKNFKY"
     gene            complement(2994..3524)
                     /gene="lptA"
                     /locus_tag="ACFNML_001769"
     CDS             complement(2994..3524)
                     /gene="lptA"
                     /locus_tag="ACFNML_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HFC6393101.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLDQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRGGKGGESVRAEGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVVIQPSSTQKSE"
     gene            complement(3505..4086)
                     /gene="lptC"
                     /locus_tag="ACFNML_001770"
     CDS             complement(3505..4086)
                     /gene="lptC"
                     /locus_tag="ACFNML_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFC6393102.1"
                     /translation="MKVRWRYGIAFPLILAVALGSLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSSKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(4083..4619)
                     /locus_tag="ACFNML_001771"
     CDS             complement(4083..4619)
                     /locus_tag="ACFNML_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HFC6393103.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
BASE COUNT         1049 a         1259 c         1224 g         1152 t
ORIGIN      
        1 ctgaaaacag tttagacggc atcgttccca tcttcgcgcc gccgttataa aatgaccgcc
       61 ccgccttgat ggaatatgac tatgcacgcc ctcccccgct acgccgtttt tggcaacccc
      121 gtcgcccaca gcaagtcgcc gcagattcat caacaatttg cccttcagga aggcgttgac
      181 attgaatacg aacgcatttg cgccgacatc ggcggtttcg cgcaggcggt ttcgacattt
      241 tttgaaacag gcggttgcgg ggcaaacgtt accgtaccgt tcaagcagga agcgtttcat
      301 ctggcggacg agcattctga acgcgcattg gctgccggcg cggtcaacac gctgattttt
      361 ctgaaaaacg gaaaactgcg cggcgacaat accgacggta tcggtttggc caacgacatc
      421 acgcaggtca aaaacattgc catcgaaggc aaaaccatct tgcttttggg cgcgggcggc
      481 gcggtgcgcg gcgtgattcc tgttttgaaa gaacaccgtc ctgcccgtat cgtcattgcc
      541 aaccgcaccc acgccaaagc cgaagaattg gcgcggcttt tcggcattga agccgtcccg
      601 atggcggatg tgaacggcgg ttttgatatc atcatcaacg gcacgtccgg cggcttgagc
      661 ggtcagcttc ctgccgtcag ccgtgaaatt tttcgcaact gccgccttgc ctacgatatg
      721 gtttacggcg acgcggcgca ggcgtttttg aactttgccc aaagcaacgg cgcggccgaa
      781 gtttcagacg gcctgggcat gctggtcggt caggctgccg cctcctacca tatttggcgc
      841 gggtttacgc ctgatatccg ccccattatc gaatacatga aagccctgta accatgttcc
      901 gcatcatcaa atggctgatt gccctgcccg tcggcatctt tatctttttc aatgcctatg
      961 tgtacggcaa catcattacc taccgcgccg tcgcgcccca tcagactgcc tttatgtcga
     1021 tgcggatgaa gcagtttgaa caggaaggtc gcgatgtcgc actggattac cgctggatgc
     1081 cctacaaccg catctcggtc aacctgaaaa aagccctgat tgcttccgaa gatgcccgtt
     1141 tcgccgggca cggcggcttc gattggggcg gcattcaaaa cgccatcagg cgcaaccgga
     1201 acagcggcaa agtgaaggcg ggcggctcga ccatcagcca gcagcttgcc aaaaacctct
     1261 tcctcaacga aagccgcagc tatatccgca aaggcgaaga agcggcgatt accgcgatga
     1321 tggaagccgt taccgacaaa gacaggattt ttgaactgta tttaaactca atcgaatggc
     1381 actacggcgt tttcggcgcg gaagccgcgt cccggtattt ttatcaaata cccgccgcca
     1441 agctgaccaa acagcaggcg gcaaaactga cggcgtgcgt ccccgccccg ctctactacg
     1501 ccgaccatcc gaaaagcaaa cggctccgca acaaaaccaa tatcgtgctc agacgcatgg
     1561 gttcggcaga gttgcctgaa agcgacacgg actgattgtt tcagatatgg aaatgccgtc
     1621 tgaactgcgg ttcggacggc atatgttttc tggtttttat agtggattaa caaaaatcag
     1681 gacaaggcga cgaagccgca gacagtacaa atagcacgga atcgattcac ttggtgcttc
     1741 agcaccttag agaatcgttc tctttgagct aaggcgagcc aacgctgtac cggtttaaat
     1801 ttaatccact atacctgccc gacagccgca aagaccgtat catacatagc aggcattgcg
     1861 gtgcgggatt actgccgcaa acggcaggca aaaaaaataa ccgcttcaag gcggtttttt
     1921 ggtggccaga ggcggaatcg aaccgccgac acacggattt tcaatccgtt gctctaccaa
     1981 ctgagctatc tggcctttgt ctgtctcgtt aagagatgcg tattaaacca aattacggga
     2041 cgacatgcaa gcatatcggg caatattctt tgaaaacagg tttaagtcaa tgttttaaat
     2101 ttgaattaat ttatgtaaaa acaacataaa tccgtatttt tgccgcctgc ccgacgatat
     2161 taggtcgccc gtctgaaaaa tattttcaat atttgaagtt cttacccaga taaacagaac
     2221 gaacctgttc gtttccgacc aaatcatcag gttttcccga tgccagcacc gcaccgtctg
     2281 aaatgatgta ggcgcggtcg cagatgctga gggtttcgcg tacgttgtgg tcggtaatca
     2341 gtacgccgat accgcgcgac ttgagaaatc cgatgatttt ttggatgtcg atgacggcga
     2401 tgggatcaac accggcaaaa ggctcatcca gcaaaataaa atgcggtttc atggcgagta
     2461 cgcgggcgat ttcgacgcgc cgccgttcgc cgccggacag cgacggcgcg gggctgcggc
     2521 gcaagtgtcc gatattaagg tcggcgagca gtttttcgat ttccctgtcg atttgatttt
     2581 tatctttggt tctgatttcc aagatggcgc ggatgttttg ttcgacggtc attttgcgga
     2641 atatcgaggc ttcctgcggc aggtagccga caccgaggcg ggcgcgttcg tgtatgggca
     2701 ggtggcgcaa ttcttgtccg tctagggtta cgctgcctgc gtcggcggca atgagtccga
     2761 ctatcatgta gaagctggtg gttttacccg cgccgttggg cccgagcagg ccgacgactt
     2821 ctccgctttc gatttcgagg gagaagcttt taacgacttg gcgttttttg aaactttttt
     2881 gcaggttttg aacaacaagg cggctgacgt ttgcgctcat aaatatctct tcaatatctt
     2941 cggtcggcaa atgccgtccg aaccaggttt atgtttcaga cggcattttg ggattattcg
     3001 gatttttgcg tactcgaagg ctggataacg acgctgaccc tgccggattt ggaagcggat
     3061 tttgcgccgg attttgtgct gccgctgatg gtatagactt cggttttggt gttgtatgta
     3121 atcaccgcac cttcggcgac atcgccgccg cgctgtactt tggcattacc ggttaagact
     3181 acggtgctgc ctgcagatga ataagcaacg ttgtttgcct gtccgcgcac cgtgcctttg
     3241 ccgccgtcca atgtctggct gaagcggact ggcgaacctt ccgccctcac ggattcgccg
     3301 cctttgccgc cgcgtgtaac attgacgcgg gcggcggaaa tattgagcgt accctgtctg
     3361 atgacgacgt ttccgctgaa tgtggtgctt tggttggctt gatcgagcga accttggtcg
     3421 gcctcaatct gaataggctg cctgctgtcg ctttgaaggg caaaagcggg ggacgccgaa
     3481 aaaaatgcta ttaaaacaaa tagcttacat atcttttgta tcataaatcg tggctttcac
     3541 tttagatgag aagttcaaca tgcctgtttt gtggtcgtaa gtcatgccgc ccgcctgacc
     3601 gtgcgatgca ccatattgga aactgacagg cgtatcggtt tgggcatatt gagattcggt
     3661 atcgacgtgc agcttttcgg cttcaacttt acccgcctgc cgtttgccgt cggcggtttt
     3721 ggtcagcaca acgttgtttt taaaaagaac ctgtttgttt tcggtatggt aaacggcttc
     3781 atcgctgccg acttcgtaca acaacctgcc ttcttggaag aacacgagat gcggtgagtc
     3841 gaaatgaatg tcgctgcttt cgggaaactg tttcgcgccc ttcgaactca aatgttcttt
     3901 caagtatccc tgttcgtcaa accgcctgcc atccaatccg tccattgtgt attgcggttc
     3961 gtcgggattg agcctgactt cttcaatctc gacttcgctg atgcgtccca accatgccga
     4021 caggctgccc aaggcaaccg ccaatatcaa tgggaacgca attccgtacc gccatcttac
     4081 tttcatttga tgtactcgtt caaagccgcg cccaaagtcc cttgcgcctg cataatcagg
     4141 tcgcacactt cgcgcaccgc gcccgcaccg ccggggcgtt tggcaatata ggcggcgtgt
     4201 tgcagcgtaa accaatgcgc gtcggggacg gcaaccggca atccgcagcg taccataacg
     4261 ggcaaatcga ccacgtcgtc gccgacaaag gcacactcgg cttcttccac gccgacctga
     4321 gcgcgcagct cttcatacgc ggcgcgtttg tccgaaatgc ctttgaagta gtaatttatg
     4381 cccaactgtt tgacgcggat gccgacggag ggcgcgtccc gacctgtgat aatcgcagtc
     4441 tgcacgccgc ttgcctgaag cattttcaga ccgtgtccgt ccagtgtgtg aaacgatttg
     4501 atttcttcgc cgttatcgcg gataaagatg cgcccgtcgg tcaaaacgcc gtccacatcc
     4561 aggatcaaca gtttgatttt ggcggcgcgc gcctgtaatt cgggagaaat tgcctgcatg
     4621 ggatttcctt gttttcagac ggcatatctt accacaaatg ccgtctgaaa cggctgcaac
     4681 gcct
//
LOCUS       DAXHTR010000144         4613 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.144, whole genome shotgun sequence.
ACCESSION   DAXHTR010000144 DAXHTR010000000
VERSION     DAXHTR010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4613
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.144"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            50..473
                     /locus_tag="ACFNML_001772"
                     /pseudo
     CDS             50..473
                     /locus_tag="ACFNML_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            579..1142
                     /gene="pgsA"
                     /locus_tag="ACFNML_001773"
     CDS             579..1142
                     /gene="pgsA"
                     /locus_tag="ACFNML_001773"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFC6393104.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLTAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1222..1297
                     /locus_tag="ACFNML_001774"
     tRNA            1222..1297
                     /locus_tag="ACFNML_001774"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1255..1257,aa:Gly,seq:gcc)
     gene            1323..1398
                     /locus_tag="ACFNML_001775"
     tRNA            1323..1398
                     /locus_tag="ACFNML_001775"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1356..1358,aa:Gly,seq:gcc)
     gene            1422..1497
                     /locus_tag="ACFNML_001776"
     tRNA            1422..1497
                     /locus_tag="ACFNML_001776"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1455..1457,aa:Gly,seq:gcc)
     gene            1519..1594
                     /locus_tag="ACFNML_001777"
     tRNA            1519..1594
                     /locus_tag="ACFNML_001777"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1552..1554,aa:Gly,seq:gcc)
     gene            1620..1693
                     /locus_tag="ACFNML_001778"
     tRNA            1620..1693
                     /locus_tag="ACFNML_001778"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1652..1654,aa:Cys,seq:gca)
     gene            1796..1885
                     /locus_tag="ACFNML_001779"
     tRNA            1796..1885
                     /locus_tag="ACFNML_001779"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1830..1832,aa:Leu,seq:taa)
     gene            1989..3041
                     /gene="sohB"
                     /locus_tag="ACFNML_001780"
     CDS             1989..3041
                     /gene="sohB"
                     /locus_tag="ACFNML_001780"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFC6393105.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSAETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3092..4062)
                     /locus_tag="ACFNML_001781"
                     /pseudo
     CDS             complement(3092..4062)
                     /locus_tag="ACFNML_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
     gene            complement(4073..4189)
                     /locus_tag="ACFNML_001782"
                     /pseudo
     CDS             complement(4073..4189)
                     /locus_tag="ACFNML_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
     gene            complement(4328..>4613)
                     /locus_tag="ACFNML_001783"
     CDS             complement(4328..>4613)
                     /locus_tag="ACFNML_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6393106.1"
                     /translation="DKGYDSAENRQHLEEHQLQDGIMRKACRNRPLTEAQTKRNRYVV
                     EQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT         1153 a         1117 c         1188 g         1155 t
ORIGIN      
        1 tccactatat tatcgctggg tatggtttgc cgccataagc tgtctgctgt tgtccgccgt
       61 ttttattgca ccttacctga cggcatttca cgaacaagaa aaaacattcg aatatgcgga
      121 attgaccgtt accgccctca accgcagcgg acgggcaatc aaactggatg cagacgggca
      181 gcattaccgg ctttcctgct atgggttcga cagtttgtgc acaggcggca atatcggcag
      241 aaccatcagg gcggagcagg ttagagccgt tttaagcgaa accgtcggca aaggtttttt
      301 aaacggcgtt ctgttggaat accgcaacag tggcagtgtc tataagcaat aaagattttg
      361 cccgcacgga agaccgcctt gtcgaagtgt tggcacaacc tgccgttttc agcctgaaac
      421 tgggtatttt gcttttattg cccgccattt tcctgcgttt gaaaagaatg tgaaaacaga
      481 ttggcggggc gggggaatgc ccatgcagca attttacgtt ttgggttatg aggataaccg
      541 ttataatcac aactttacag tctgcacaga gaaaaccgat gccttggaat ctccccatct
      601 tcctcacatg gttgagggtc ttgctcattc ctgtcatgac agcccttttt tacctgcctt
      661 ttccgtggtt cgcggaggaa acgattaatc tcaccgccgc cgtcattttt gccgttgccg
      721 ccttaaccga ctggtttgac ggatttttgg caagactgtg gaaacagact tccgatttcg
      781 gcgccttcct cgatcccgtc gccgacaagc tgatggttgc cgtatcgcta cttttactgg
      841 tcaaactcga ccggacctat gttttgtttg ccatgatcat tatcggcagg gaaattacca
      901 tttccgcatt gcgcgaatgg atggcgcaaa tgggcaaaag gaacagcgtt gccgtcgcca
      961 ccgtcggtaa gttcaaaacc gccgcgcaaa tgctggcaat cttcctgctg ctgctgaata
     1021 ttcccgattt ttacggattt aatcttgttg ttatcggcaa tgtattgatg tttatcgcat
     1081 ctttgctgac agtctggtcg atgctgtatt atctgaaaat ggcgtggaaa gaaatcgcct
     1141 gaaaaaaaca taaaaatagc ttgacggcaa aaacagaatc cataataatt gcgtctcttc
     1201 gatgttggag aatgaaatcg ggcgggaata gctcagttgg tagagcgcaa ccttgccaag
     1261 gttgaggtcg cgagttcgag actcgtttcc cgctccaaaa atttcatttt cccacaatca
     1321 ttgcgggaat agctcagttg gtagagcgca accttgccaa ggttgaggtc gcgagttcga
     1381 gactcgtttc ccgctccaag ttttatttca aacatatcaa cgcgggaata gctcagttgg
     1441 tagagcgcaa ccttgccaag gttgaggtcg cgagttcgag actcgttttc cgctccaaaa
     1501 accgatatgt ttgaacgcgc gggaatagct cagttggtag agcgcaacct tgccaaggtt
     1561 gaggtcgcga gttcgagact cgtttcccgc tccaagtttt ttagataagc cagttttagg
     1621 gcgagatagc aaagtggtta tgcagcggat tgcaaatccg tctacgccgg ttcgattccg
     1681 actctcgcct ccattatcgt actatggcgg ggtggcagag tgtttatgct atgaggagtg
     1741 caatcttcat ataggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg
     1801 ggtggtgaaa taggtagaca caacggactt aaaatccgtc gggactaaac atcccgtgcc
     1861 ggttcgattc cggctccggg caccaagctg aaaatgacaa tgccgctcca agcggttatt
     1921 tttttatctg tcggacggtg atggacaata ttgacatgtt catgcctgaa caagaggaaa
     1981 tccaatcaat gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta
     2041 ccgtgttcgg cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg
     2101 aaagcggcag tgtcgtactg acggattttt cggaaaatta taaaaaacag cggcaatcgt
     2161 ttgaagcatt ctttttaagc ggggaagagg caaaacatca ggaaaaagag gaaaagaaaa
     2221 aggaaaaggc ggaagccaaa gcagagaaaa agcgtttgaa ggagggtggg gagaaatctg
     2281 ccgaaacgca aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg
     2341 ccgtagaatc cttacgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg
     2401 aggttctgct tagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt
     2461 cacagcttag gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtgg
     2521 cggcgagcgg cggttatatg atggcgtgtg tggcggataa aattgtttcc gctccgtttg
     2581 cgattgtcgg ttcggtgggt gtggtggcgg aagtaccgaa tatccaccgc ctgttgaaaa
     2641 aacatgatat tgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta
     2701 tgggtgaaaa tacggaaaag ggcaaacaga aattccgaca ggaactggag gaaacgcatc
     2761 agttgttcaa gcagtttgtc agcgagaacc gccctcaatt ggatattgag gaagtggcaa
     2821 cgggcgagca ttggttcggt cggcaggcgt tggcgttgaa cttgattgac gagatttcga
     2881 ccagtgatga tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc
     2941 aggagaagca aagcctgatc cagcgcattg gtttgcaggc ggaagcttct gttgaaaagt
     3001 tgtttgccaa acttgtcaac cggcgggcgg atgtgatgta gtttgccgaa cggatggaaa
     3061 tgccgtctga agcgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgttccg
     3121 accactctat ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca
     3181 cgtcgtccaa actcatggca aacatccaca gataagtgcc gaccgaatag acatgccccg
     3241 cgctgctgta gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg
     3301 ccgtgccgac gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg
     3361 caagcagtcc gtaatggcgg tacagctgcc gccggtcgcc ttttcggata aagtggttgt
     3421 cgcgttccaa gctgttcttc aggcggaaat acaggttttc gctgatggcc gcaaaacgtg
     3481 gcaaaagcca taaaaacaac gcaagtatgc ccaccgccga cacgccgacc caaaattcca
     3541 gcaccagcag catgatgcac gcgccgaata tggatacgac ggatgtcgcg gcaatcggca
     3601 ggtgttcttc aaaaaagctg acaaactcac gcgacagggc aacccgcgca gttatagtgg
     3661 attaacaaaa atcaggacaa ggcgacgaag ccgcagacag tacaaatagt acggcaaggc
     3721 gagacaacgc cgtactggtt taaatttaat ccactatatt gccgaatgcg ggacttaccg
     3781 ctgccgctgt tccaacacaa ccggcacggc gatttcggta taaatccgcg taaacgtgcg
     3841 tgtatcggca atccgccgcg ccgcaccgac cagccacatt aaaagcacaa ccaaagcgta
     3901 cagcaacgcc tgccacacct cccccgcaat cacggcattg atcgcccggc cgccaaacac
     3961 cggatacacc agcatcaaaa ggttttccag tccgaccagg gaaaatgtgc caatcaatcg
     4021 cttgcggtgg gtttgggcta tgtgtttcaa cattttccac atatttgccc ccctattttt
     4081 tattttccga gattgttttt tccatcactt cagccagtgc atccaaatcg gcaaacagcc
     4141 gggttgtgcg cttgtcgccg aatgtggcaa atactttgtg agacctttgc aaaattcctt
     4201 tccctcccga cagccgaaac ccaaacacag gttttcgtct attttcgctc ctaattactc
     4261 ctaattctac ccaaataccc ccttaatcct caccggacgc ccgataatta ggcatccggg
     4321 ctgcctttta ggcggcagcg ggcgcactta gcctgttggc ggctttcaac aggttcaaac
     4381 acatcgcttt cagatggctt tgcgcactca ctttaatcag tccgaaatag gctgcccggg
     4441 cgtagcggaa tttacggtgc agcgtaccga agctttgttc gaccacataa cggttgcgtt
     4501 tggtttgtgc ctccgtcagc ggacggttgc ggcaggcttt gcgcataatg ccgtcctgca
     4561 actgatgttc ttccagatgt tgccggtttt ccgcactgtc gtagcctttg tcg
//
LOCUS       DAXHTR010000145         4579 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.145, whole genome shotgun sequence.
ACCESSION   DAXHTR010000145 DAXHTR010000000
VERSION     DAXHTR010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4579)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4579)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4579
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.145"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(181..1119)
                     /locus_tag="ACFNML_001784"
     CDS             complement(181..1119)
                     /locus_tag="ACFNML_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFC6393107.1"
                     /translation="MLELNGLCKRFGNKTVADDICLTVGRSKILAVLGRSGCGKSTLL
                     NMIAGIIRPDGGEIRLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAESLAMAALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRKGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVAHLMGLPNTDDDRHIPQHAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMRYAGAVSGKDTVRIHIEEREIVRFR"
     gene            complement(1128..2003)
                     /locus_tag="ACFNML_001785"
     CDS             complement(1128..2003)
                     /locus_tag="ACFNML_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HFC6393108.1"
                     /translation="MPTVRFTESVSKQDLDALFEWAKASYGAKSCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWTDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGRRSPHKAVDPDK
                     LDNTAAGGVSSGELPSETVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAILPETFLPENQDGEVACFEKMDIGGLLDAMLSENMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2192..2268)
                     /locus_tag="ACFNML_001786"
     tRNA            complement(2192..2268)
                     /locus_tag="ACFNML_001786"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2232..2234),aa:Met,seq:cat)
     regulatory      2418..2517
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2610..4511
                     /gene="thiC"
                     /locus_tag="ACFNML_001787"
     CDS             2610..4511
                     /gene="thiC"
                     /locus_tag="ACFNML_001787"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFC6393109.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTRPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTDKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1094 a         1300 c         1217 g          968 t
ORIGIN      
        1 gttgtcgctt cggataaatt tttgccgtgt tgggttctag attcccgctt ttgcgggaat
       61 gacggcaggg tggtttctgt tgtttcggat aaattttgca gccctgataa aaaatatggc
      121 tgctttggta aaaaaatgcc gtctgaaagg ttttcagacg gcatttgttt ttaagaagca
      181 tcagcggaag cggacgattt cccgttcttc gatatggatg cgtaccgtat ccttgcccga
      241 taccgccccg gcgtaccgca tatcgaggtt cagccatagg atgccgtgtt ccggatggag
      301 gacggacagg ctgaacgatt cgggcaaaca ggtacgggat aatacgcggc actccatgcc
      361 gtcttggtcg aaacgcaccg catgttgcgg aatatggcgg tcatcgtcgg tattgggcag
      421 ccccatcaga tgggcgacct gcacgccggc aggcgtttga accaaggttt cgggcgtacc
      481 gcattgaagg attttcccct cgtgcatgac ggcgatttcg tctgccgccg tgcaggcctc
      541 ttcgggcgaa tgcgttacca aaacggcagg gatgccgccc ttgcggatac gttcggcagt
      601 catgcggcgc agccggtcgc gcaaatgcgt gtccaaactg gaaaacgatt cgtccaacag
      661 cagcagggaa gggcggacaa ccaaagcgcg cgccagtgcc aaccgttgct tttcgcctcc
      721 ggaaagtttt tcaggcttgc ggtgcgcctc gttttccagt ccgacttcgg caagtgccgc
      781 catggcgagg ctttcggctt cggctttcgg cattttttgc attttcaaac cgaatgccgc
      841 attttccagt gcactcatat ggggaaacag cgcgtaatct tgaaacatca gcgaaatacg
      901 gcgtttttcg ggcggcatac gggtaatgtt ttccccgttc agccgaattt cgccgccgtc
      961 cggacggata atccccgcaa tcatattcag cagggtggat tttccgcagc ccgaccgtcc
     1021 caaaacggca agtattttgc tgcggccgac agtcaggcag atgtcgtcgg cgacggtttt
     1081 attgccgaag cgtttgcaga gtccgttcag ttcaagcatg gcgtatccta taaacgtatg
     1141 ccgtccagcc actcggacag cggatgggcg gcatcaatca gaccgtaacg gcaaaacgcg
     1201 tccagcgtaa ccagttgcgc gtcgtgcatc atgttttccg acaacatggc atccaacaga
     1261 ccgccgatgt ccatcttttc aaaacacgca acctcgccgt cctgattttc aggcaggaag
     1321 gtttcgggca ggatggcatc gaatacatac aggatttcat tgtgcacacc ccggctgacg
     1381 gggcgcaggc tgtgcagccg agataccgga cggataaacg ggaacagcgt tttatccaaa
     1441 ccggcttctt cgctgctttc gcgacacacg gtttcagacg gcaattcacc gctggaaaca
     1501 ccgccggcgg cagtattgtc gagtttgtcg ggatcgactg ctttgtgcgg actgcgcctg
     1561 cctatccaga aatgccatcg gccgtccgat tcggtcagac cgttgagatg gacggcgcgg
     1621 ctgagtagtc cgaacgggcg gaaagcggcg cgttcgagcg tgaacaaggg gttgccgccg
     1681 ccgtcggtca ggtcgaaaca ctcgttgcgc cagccgtgaa gcagccccgc cttgttccat
     1741 gtgcgggcga ggtgctgcaa gcgtccgccc atatccgtcc agccgtccgc attcagaaaa
     1801 atgccgtctg aagactccga gcagcctgcc tcccagtctt ttttgacgcg ctccgcccat
     1861 tccggcgaca gattgcccaa aggcagacgg ttcagataca gcgttttcca gcaacttttt
     1921 gcaccgtaac ttgcttttgc ccactcgaac agggcatcaa ggtcttgttt gctgacggat
     1981 tcggtaaaac ggacggtcgg catagtcggc tttcttgaac ataatgcgga agattgtagc
     2041 caaaccgcct gaatttgcaa ccgcaaacag acggcgggct gccgttccga tattgcggca
     2101 ggaaggaaaa tccggcaaaa aacggaggcg gcaggaaaca ggaaacaggc aaaacaaaac
     2161 ccctcggcat tgaaccgagg ggtttaatat ttggttgcgg gagcaggatt cgaacctacg
     2221 accttcgggt tatgagcccg acgagctacc atgctgctcc atcccgcgtc agaagatgga
     2281 actatacggc agatttttta tgttgtcaaa cattatttcc gcgaaaaatc ataaaatttt
     2341 gccgtccggc ggtttatgcc tgatttgaaa tatcattttc tttacaaaag ttcatgttcg
     2401 tgatttaatt ttggttaaca ttgaaacagg ggtgctgcat gatgttcagg cggctgagaa
     2461 atacccttta cacccgatct ggataatacc tgcgtgggga gttttcacgg attctgcctt
     2521 tcagacggca ttttttgata ctcgggccgt ttgaaaaagc aaagtgctcc tgtttcttta
     2581 ttctaaacga gaaaacagga gcatttttta tgactacgcc aaaaaaaact gccaaaactt
     2641 ccggcaacga agcgcgcgaa cttgccgact tgagcgaaga catcggcatc cgcttcaaat
     2701 atccgaactc ggagcgcgtg tatctgcaag gcagccgcga cgacatccgc gtgcctttgc
     2761 gcgaaatccg tcaggacgac acctacacgg cgcaaggcac ggaagccaac ccgccgattc
     2821 ccgtctatga caccagcggc gtgtacggcg atccggcggc gcacatcgac ctgaaacaag
     2881 gtctgccgca catccgcacg gcgtggctgg acgaacgcgg tgataccgaa atcctgccca
     2941 agctttccag cgaatacggc atcgaacgcg cacacgatcc gaaaaccgcc catctgcgct
     3001 tcaaccaaat cacccgcccg cgccgcgcca aagccggccg caacgtaacc cagctccatt
     3061 acgcccggca aggcattatc acgccggaaa tggaatttgt cgccatacgc gaacgtttga
     3121 aattagacga attgtcccaa aaaccggaat acgccaaact cttgaagcaa cacgcggggc
     3181 aaagtttcgg cgcgaacatc ccgacccgtc ccgatcaaat cacacccgaa ttcgtgcgcc
     3241 gggaaatcgc cgccggacgt gcgattattc ccgccaacat caaccatccc gaactcgaac
     3301 cgatgattat cggccgcaac ttccgcgtca aaatcaacgg caacttgggc aattccgccg
     3361 tcacttccag cctgaccgaa gaagtcgaaa aaatggtgtg gtcgctgcgt tggggcgcgg
     3421 acacaattat ggacttatcc acaggcgcgc acatccatga aacgcgcgaa tggattatcc
     3481 gcaacgcgcc cgtccccatc ggcaccgtgc ctatttatca ggctttggaa aaaaccggcg
     3541 gcattgccga agatttgact tgggatttgt tccgcgacac cttaatcgag caggcggaac
     3601 aaggcgtgga ctatttcacc atacacgcgg gcgtgttgct gcgttatgta ccgatgaccg
     3661 ccaaccgcct caccggcatc gtctcgcgcg gcggttcgat tatggcgaaa tggtgtttgg
     3721 cacatcatcg ggaaaacttc ctctacacgc atttcgacga aatctgcgaa atcatgaaag
     3781 cgtatgatgt gtcgttcagc ctcggcgacg gcctgcgccc cggctgcatt gccgatgcca
     3841 acgacgaatc ccaattcgcc gagctgcaca ccttgggcga attgaccgat aaagcgtgga
     3901 aacatgacgt acaagtcatg atcgaaggcc ccggccatgt gccgctgcaa cgcgtcaaag
     3961 aaaacatgac cgaagagctg caacactgct ttgaagcacc tttttacaca ctcggcccgc
     4021 tcgttaccga catcgcaccc ggctacgacc acatcacctc gggcataggc gcggccaata
     4081 tcggctggta cggcacggcg atgctttgtt acgttacccc caaagaacat ttggggctgc
     4141 ccgacaaaga agacgtgcgc accggcatca tcacctacaa actcgccgcc cacgccgccg
     4201 atctcgccaa aggctggccg ggcgcacaat tacgtgacaa cgccctgagc aaggcgcgtt
     4261 tcgagttccg ttggcgcgac caatttcgct taagccttga ccctgaacgt gccgagagct
     4321 tccacgacga aaccctgcct gccgaaggcg cgaaaatcgc ccacttctgc tcaatgtgcg
     4381 gccccaaatt ctgctcgatg aaaatcacgc aggaagtgcg cgactacgcc gacaagcaaa
     4441 aagcccaacg gcagggcatg gaggaaaaag cggtcgagtt cgtcaaaaaa ggcgcgaaga
     4501 tttacagttg aaacgtcaag caaaaaatgc cgtctgaaac ccggaaaaaa ggcttcagac
     4561 ggcattcttt cgcttgtga
//
LOCUS       DAXHTR010000146         4574 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.146, whole genome shotgun sequence.
ACCESSION   DAXHTR010000146 DAXHTR010000000
VERSION     DAXHTR010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4574
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.146"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            123..2150
                     /gene="uvrB"
                     /locus_tag="ACFNML_001788"
     CDS             123..2150
                     /gene="uvrB"
                     /locus_tag="ACFNML_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFC6393110.1"
                     /translation="MEVIQYPNSPFKLHQPFPPAGDQPTAIAGLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRDDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIAQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTVFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERVLVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEAAVMRDRIRNIKESLLFGAE"
     gene            2196..3464
                     /locus_tag="ACFNML_001789"
     CDS             2196..3464
                     /locus_tag="ACFNML_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT domain-containing protein"
                     /protein_id="HFC6393111.1"
                     /translation="MKLSKTRIDKSGLALAKQQYKDEIDFIELEECFDLYRQSHLSPL
                     SKTTIDIQNILREYIGEYYIAQRLKRKPQIIRKLNRLSVRLTQLQDIGGCRIIVPTNR
                     DVDKIYKFLSSKKDNLDFLIKKVTDYREKGRDDSGYRALHIIIERENLNFELQIRSRI
                     QHYWAESIEKTSVVYGYYLKEQEGDRDVINYFKELSDVFYEIESGRKPTTIQKLNLED
                     LRKRCEVIIQKSDTHKILNSYVNEGIISALIEKEKRNTNSLNNWILIFDWNAGVFVSW
                     DVVAQDPKQAVEVYVNYENQYPSLEGFEVVLIGSSDIASVQKTHSHYFGIASYENILE
                     TLDESIFCFAKKDDIDIGARRILAAMVRHKFWGTKTVTSETLKNHFCQNLFGFDYSLR
                     DLIRKELIKYGRDKDLSLNIAKKMEIESYL"
     gene            3874..4359
                     /locus_tag="ACFNML_001790"
     CDS             3874..4359
                     /locus_tag="ACFNML_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6393112.1"
                     /translation="MPSFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRERY
                     PFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSKYLIGGNLRLWQKPFWEHTI
                     RDEADFACHFDYLHFNPVKHGYVGQISDWRFSTFHRYVKQGIYPHNWGGGNADFSIGY
                     D"
     gene            4396..4574
                     /locus_tag="ACFNML_001791"
                     /pseudo
     CDS             4396..4574
                     /locus_tag="ACFNML_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         1390 a         1086 c         1002 g         1096 t
ORIGIN      
        1 tgggtagcga ttgggtttca gacggcatac attcaggatt tcaccgcctt atgccgtctg
       61 aaagacgttt ccgacccatc cctactacac actttgtccg caaacgcatc acaggcaaga
      121 ttatggaagt tatccaatac ccaaattccc ccttcaaact ccaccaaccc ttcccgcccg
      181 caggcgacca gcccaccgcc attgcaggcc tgctcgaagg gctttcagac ggccttgcct
      241 atcaaaccct gctcggcgta accggttcgg gcaaaaccta caccatggcg aacgtcatcg
      301 cccaaagcgg ccgccccgcc atcatcatgg cgcacaacaa aacccttgcc gcccagcttt
      361 acgccgaaat gcgcgaattt ttccccgaaa acgcagtgga atatttcgtc tcctactacg
      421 actattacca gcccgaagcc tatgtgccca gccgcgactt gttcatcgaa aaagacagcg
      481 cgatcaatga acacatcgaa caaatgcgcc tttccgccac caaaaacctg atgacgcgcg
      541 acgacgtgat tatcgtcgcc accgtgtccg ccatttacgg tatcggcgac ccgaccgagt
      601 atcaacaaat ggtgttgtcc gtcaaagaag gcgataccat agcccagcgc gacatcatcg
      661 ccacgctcgt ttccatgcag tacgaacgcg gcgatttgga cttcaaacgc ggcagcttcc
      721 gcgtgcgcgg cgacgtgatt gacgtgtacc ccgccgaaag ctccgaaaac gccttgcgta
      781 tcagcctgtt cgacgacgaa atcgaccgcc tcgatatgtt cgacccgctt tcaggcagcc
      841 ttattcaacg cgtcggccgc tacaccgtct tcccgtccag ccactacgtt accccgcgcg
      901 acaccgtatt gcgcgcctgc gagtccatca aagaagaatt gcgcgaacgc atcgaatttt
      961 tcgcccgcga acaacgcccc gtcgagcaac aacgcatcga acaacgcacc cgcttcgacc
     1021 tcgaaatgct ctacgaaatg ggcttctgca aaggcatcga aaattattcc cgccacttct
     1081 ccggcaaaaa agaaggcgaa ccgccgccca cgctgatgga ctatctgccc gacaacgcca
     1141 tcatgttcat cgacgaaagc cacgttaccg ttacccaaat cggcggcatg tacaaaggcg
     1201 acgcatcgcg caaacaaaac ctcgtcgact acggcttccg cctgccttcc gcccgcgaca
     1261 accgcccgct caaattccac gaatttgaaa aagtcatgcc gcaaaccgtc ttcgtttccg
     1321 ctactcccgc caaatacgaa gaagaacacg ccggacaagt ggtcgaacaa gtcgtccgcc
     1381 ctacagggct ggtcgacccc caaatcatca tccgccccgt cgccacccaa gtcgatgact
     1441 taatgagcga aatcaacgac cgcatccaaa aaggcgaacg cgttctcgtt accaccctca
     1501 ccaaacgcat ggcggagcaa ctcaccgact attacagcga actcggtatc aaagtgcgct
     1561 acctgcacag cgacatcgac accgtagagc gcgttgaaat tattagagat ttgcggcttg
     1621 gactgtttga cgtacttgtc ggcatcaacc tcttgcgcga aggcttggac atccccgaag
     1681 tctcccttgt cgccatcctc gacgccgaca aagaaggctt cctgcgctcg caccgcagcc
     1741 tgattcaaac cataggccgc gccgcgcgca acgtgaatgg cgttgcaatt ttgtatgccg
     1801 acaaaatcac cgactccatg aaagctgcca tcgacgaaac cgaacgccgc cgcgaaaaac
     1861 aaattaaatt caacgaagaa cacggcatcg tgccgcagca gattaaaaaa caggtcaaag
     1921 acatcatcga cggcgtgtac cacgaagaag acagcggcaa aggccgtaga caaggcaaaa
     1981 acaaagttaa agtcggggag attcataacg aagaagacgc gattaaagaa atcgccaaac
     2041 tggaaaaagc catgcagcag gcggctcggg atttgcagtt tgaggaagcg gcggttatgc
     2101 gtgatagaat acggaatatt aaggaaagcc tgttgtttgg ggcagagtaa tgaaatagtt
     2161 taatattctc agcttaatgt ataaaggata attttatgaa attgtcaaaa acaagaatag
     2221 ataaatctgg cttagcattg gctaaacaac aatataaaga tgaaattgat tttattgaat
     2281 tggaagaatg ttttgatttg tataggcaat cacatctttc accattatct aaaacaacta
     2341 ttgatattca aaatattctt agggaatata ttggtgagta ttatatagct cagcgactaa
     2401 aaagaaaacc acagataatc agaaagttaa ataggcttag cgttagacta actcagttgc
     2461 aagacattgg tggttgtaga attattgttc caacaaatag agatgttgat aagatatata
     2521 agtttttatc aagtaagaag gataatcttg attttttaat aaaaaaagta accgattacc
     2581 gtgaaaaagg tagagatgat tcagggtata gagctttaca tataattata gaaagagaaa
     2641 atcttaactt tgaattacaa attagaagta ggatacaaca ttattgggca gaaagtatag
     2701 agaaaacctc tgttgtttat ggatattact taaaagagca agaaggggat agagatgtaa
     2761 ttaactattt taaagaatta tcagatgttt tttatgaaat tgaatcaggg agaaaaccaa
     2821 ctactataca aaagttaaat cttgaagatt taagaaaaag atgtgaagtt attattcaga
     2881 aatcagatac tcataaaata ttaaatagct atgttaatga aggtataatt agtgcgttga
     2941 tagaaaagga aaagagaaat actaattcat tgaataactg gatactaatt tttgattgga
     3001 atgcaggggt tttcgttagt tgggatgttg tagctcaaga tccgaagcaa gccgttgagg
     3061 tatatgttaa ttatgagaat cagtatccat ctttagaggg gttcgaggtt gttttgatag
     3121 gatcatccga tatcgcttct gtacaaaaaa cacatagtca ttattttggc atagcctcat
     3181 atgaaaatat tctagaaaca cttgatgaat caattttttg ctttgctaaa aaagatgata
     3241 ttgatattgg ggcaagaaga atattggcag caatggtaag gcataaattt tggggaacaa
     3301 aaacagtaac atcagaaact ttaaaaaatc atttctgcca aaatcttttt ggatttgatt
     3361 attctttaag ggatttaata agaaaagaat taataaaata tggaagagat aaagatttat
     3421 cattgaatat agcaaagaaa atggaaatag aatcgtattt ataagcaagc gcagcttgta
     3481 tccccatcaa ccgtgtgcgt ggctacgcca cacaccttac ctgcgtatgg cacaaaaact
     3541 ttaaaaccga ggtgtagggt gtgtgcggta cgcacgcacg cgttctttgt agtgcaaacc
     3601 actgcttcaa actttcagac gacctcaaac gccgtctgag aatctatggc ataaaggccg
     3661 tctgaaaaaa tggtttcagt acaactgcca ctggttgtaa cttgattaaa accacccttt
     3721 cagacagcct gccgttttca gcaagcgtag gtcggatact tgtatccgac aaaaccttta
     3781 acattcccat cattgcaatc cattgcagca atgcccaaaa tgtcgaattc aagaatccga
     3841 cctacaaaat cattccgagc ataatactat gaaataccgt cgttttaccg caatggcggc
     3901 acttactttt ttacggttgt aaccaataaa cggcagaaga ttttgaccga tgatgcggtg
     3961 cgtttggctt tacggcaggc ggtaatggcg gtgcgcgaac ggtatccgtt tgaaattttg
     4021 gcatgggtgt tgatgcccga ccatctgcat accatatggc ggctgccgga caatgattct
     4081 gcttattcgg aacgctggcg gcagatcaag cggcacagca aatatttaat cggcggcaat
     4141 ctcaggcttt ggcaaaaacc cttttgggaa catactatcc gcgatgaggc cgattttgcc
     4201 tgccattttg attatctgca tttcaatccg gtcaaacatg gctatgtagg acaaatttcc
     4261 gattggcggt tttctacgtt tcaccgttat gtcaaacagg gtatttatcc gcataattgg
     4321 ggtggcggca atgcggactt ttctattgga tacgattgaa gtaatgtcgg attcgagaat
     4381 ccgacctacg gaaaactgaa agagcatcgg ctgcaggacg gcattatgcg caaagcctgc
     4441 cgcaaccgtc cgctgtcgga ggcgcaaacc aaacgcaacc ggtatttgtc gaagacccgt
     4501 tatgtggtcg aacagagctt cggtacgctg caccgtaaat tccgctacgc tcgggcagcc
     4561 tatttcggac tgat
//
LOCUS       DAXHTR010000147         4532 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.147, whole genome shotgun sequence.
ACCESSION   DAXHTR010000147 DAXHTR010000000
VERSION     DAXHTR010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.147"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..634
                     /locus_tag="ACFNML_001792"
     CDS             <1..634
                     /locus_tag="ACFNML_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393113.1"
                     /translation="HPKSTIKMAKKPNKPFRLTPKLLIRVVLLICITAIGALAIGIVS
                     TFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAASSEPAQPDGTDES
                     GSGLPSPAAPKKNRVKPQPADTAQTDRQPDDAGTQAENTLKETPVLPTNVPRPEPRKE
                     TPEKQAQPKETPKEKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            674..1453
                     /gene="map"
                     /locus_tag="ACFNML_001793"
     CDS             674..1453
                     /gene="map"
                     /locus_tag="ACFNML_001793"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFC6393114.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            1608..1907
                     /locus_tag="ACFNML_001794"
     CDS             1608..1907
                     /locus_tag="ACFNML_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFC6393115.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            2145..2519
                     /locus_tag="ACFNML_001795"
     CDS             2145..2519
                     /locus_tag="ACFNML_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HFC6393116.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(2706..4172)
                     /locus_tag="ACFNML_001796"
     CDS             complement(2706..4172)
                     /locus_tag="ACFNML_001796"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HFC6393117.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
BASE COUNT         1252 a         1324 c         1071 g          885 t
ORIGIN      
        1 acaccccaaa agcacaatca aaatggcaaa aaaaccgaac aaacccttca ggctgacccc
       61 caaactcctg atacgtgtcg tattgctcat ctgtatcacc gccatcggcg cattggcaat
      121 aggcatcgtc agcacattca acccgaacgg cggcaaaacc ctccaaaccg aaccgcaaca
      181 caccgacagc ccccgcgaaa ccgaattctg gctgccaaac ggcgtagtcg gacaagatgc
      241 cgcccaaccc gaacaccacc acgccgcctc atccgaaccc gcacagccgg acggcacaga
      301 cgaaagcggc agcggactgc cgtcccctgc cgcacccaag aaaaaccggg tcaaaccgca
      361 acctgccgac acagctcaaa ccgacaggca gccggacgac gccggaacac aagctgaaaa
      421 cacactcaaa gaaacccccg tactgcccac aaacgtcccc cgtcccgaac cccgaaaaga
      481 aacacccgaa aaacaggcgc agcccaaaga aacacccaaa gaaaaagaaa cgcccaaaga
      541 aaaagaaacg cccaaagaaa accataccaa accggacacc ccgaaaaaca cgccgcccaa
      601 accccataaa gaaattctcg acaacctctt ctgacccggc acggcaggca cacccgcaat
      661 ccaaggaagc attatgaacg gcatcatcat caaaaccccc gaagaaatcg aaaaaatgcg
      721 cgagctgggc aaactcgtcg ccgaagccct cgactacatc ggacaattcg tcaaacccgg
      781 cgtaaccacc gacgaaatcg acaaactcgt ttacgactac cacgtcaacg tccaaggcgg
      841 ctatcccgcc cccctgcact acggcaaccc gccctacccc aaatcctgct gcacctccgt
      901 caaccacgtc atctgccacg gcattcccga cgacaagccg ctcaaagaag gcgacattat
      961 caacatcgac ctcaccatca aaaaagacgg cttccacggc gactccagcc gtatgtttac
     1021 cgtcggcaaa gtctccccca tcgcccaacg cctgatcgac gtaacccacg cctccatgat
     1081 ggcgggcata gaagccgtca aacccggcgc gacactgggc gacgtaggtt acgcctgcca
     1141 acaggttgcc gaaaacgccg gctattccgt cgtacaggaa ttctgcggac acggcatcgg
     1201 gcgcggtttc cacgaagccc cgcaagtgtt gcactacgga aaaaaaggac agggccccgt
     1261 tctaaaaccg ggtatgattt ttaccgtcga accgatgatc aaccaaggca aacgccacct
     1321 gcgtatcctc aacgacggct ggacggtggt taccaaagac cgctccctct ccgcccaatg
     1381 ggaacacgaa gtcttggtga ccgaaaccgg ctacgaaatc ctcaccgtca gccccgcctc
     1441 cggcaaaccc tgaaaccgga cgtatccgcc ccataaaaac aaacaatgcc gtctgaaaga
     1501 aacggcagat atgatatata atataaaaac aggcttgacc cggcacatta cgaaaacaaa
     1561 gcaaatcgga atttgccccg caaccagaca aacttaaagg aagttttatg aaaatatttg
     1621 aaaatataga agatgttaaa gccatccgta aaaagaccgg gctgaaccag atagacttct
     1681 ggggcaaggt cggcgttacc cagtctggag gctcgcgcta cgaaaccggc cgcaaaatgc
     1741 ccaaacccgt acgcgaactg ctccgcctcg tccatatcga atgcatcgat ttggcgaaag
     1801 tcaacaaaaa agatatggaa atcgccgccc tgttgaaaaa acaccatccc gacctgtatg
     1861 ccgaattgtc caaacagacc aagtccgaaa gaaaaaaaca aagttaaacc gcaacctccg
     1921 gatgcccgaa ggtttttcat ttccgaaaaa cgcaaacaat gccgtctgaa acaccggaca
     1981 ggccgccgta tcccgcctgc cgcccctgcc tcaaaccgcc gaaccgcccg aacccgcctt
     2041 tttacaaact ttatccaatt tcctgtttat ttcaggatac gccgacatta gaatgtcaaa
     2101 cagctcgaaa cgggcaaact ccacatccat ccaaaggaat aaaaatgaaa cttctgacca
     2161 ccgcaatcct gtcttccgca atcgcgctca gcagtatggc tgctgctgcc ggcacgaaca
     2221 accccaccgt tgccaaaaaa accgtcagct acgtctgcca gcaaggtaaa aaagtcaaag
     2281 taacctacgg cttcaacaaa cagggcctga ccacatacgc ttccgccgtc atcaacggca
     2341 aacgcgtgca aatgcctgtc aatttggaca aatccgacaa tgtggaaaca ttctacggca
     2401 aagaaggcgg ttatgttttg ggtaccggcg tgatggatgg taaatcctac cgcaaacagc
     2461 ccattatgat taccgcacct gacaaccaaa tcgtcttcaa agactgttcc ccacgttaat
     2521 caggcaacaa aaaacagcgt tttcagaaat gaaaacgctg ttttttgact gttccattat
     2581 tcacaaaagg gaaaaaacga ttacctgccc cgtgtaccaa aacctgccct gccggatgaa
     2641 gggcataacc ggcagggacg gcatcaacac catatggggg tacggctttt cttgaaagat
     2701 tcggcttaaa tatccaacac tttcgcggta taggcgataa tttcatccgc cctttcaggg
     2761 ttttcgttca acttgatgcc gtaacccggt accagctctt tcagacgacc ttcccaagac
     2821 ggggcgcgct cggggaagca ttggtgcatc agccggatca tcagcggcac agcggtcgat
     2881 gcgcccggcg acgcgcccag caatgcggcg agtgagccgt cggcgtgggc gacaatctcc
     2941 gtaccaaact ggagcacgcc gcctttttcg gagtcttttt taatgatttg gacgcgttgc
     3001 cctgcggtga tgagttccca gtcgtcgggg tttgcctcgg ggtagtattc cagcagggag
     3061 gcgaagcgtt cttctttggt tttacgcaat tcgcccagca ggtatttggt cagcggcata
     3121 ttcgcccagc cggcgcgcag cataggatag agattgtcca tatggatgga cagcggcaaa
     3181 tccataagcg agccttgctt gaggaagttg gaacggaagc ctgcgtaagg gccgaacata
     3241 aggtggcgtt tgccgtccac gttgcgtgtg tcgaggtgcg ggacggacat cggcggcgcg
     3301 ccgacggaag cctgcccgta cactttggcg ttgtgttgtt cggcggtttc ggggttgctg
     3361 ttgcggaaga acaggccgga cacggggaag ccgccgtagc ctttgccttc ggggatgccg
     3421 gatttttgca gcagggtcag cgcgccgccg cccgcgccga ggaagaggaa gcgggtacgg
     3481 agggtgagct gcccgtcggg gttgcgggta tcggcggttt tgagcaccca cgcgccgtcg
     3541 gattcgcgtt tgatgtcttc gacgtggcgg ttgaactcgg tttttacgcc cttgccctgc
     3601 aaatatttca ccatttggcg cgtcagccgt ccgaaatcga catccgtgcc ttcggcggag
     3661 tagttggcgg cgacgggttg gttttcgtcc cggccgcgca tcatcagcgg agcccaatcg
     3721 gaaattttgt tccgatcggt ggaaaattcc atattttcaa aaagtttttg ggttttaaac
     3781 gcgtcataac gtttttgaag ataagaacaa tggtcttcat tcatcaccaa agacatatgc
     3841 ggcacggcat tgatgaagga attgtcttcc aacttgcctt ccgcgaccag cgtcgcccaa
     3901 aactggcggc tgacatgaaa ctgttcggca atattgaggg cgcgcgccgg atcgataatc
     3961 ccatttgcac ccaacggcgc atagttcaat tcgcacagcg cggaatgccc cgtgccggcg
     4021 ttgttccacg cgtttgacga ttccaacgcc acatcttcca agcgttcaat cagggtgatt
     4081 tcccaagacg gttcgagttc tttgagcaaa acgcccaaag tcgcgctcat aatgccgccg
     4141 cccaccaaga caacgtctgt cgcttcagcc atggtttact cctaaaaaac aggcatcttc
     4201 tgcccttatg gttatttgcc gtactacaaa cgcctgaatc gcaaaagcag ggaaaaccgg
     4261 caatggtgtg tgtccgagta tgctgtttcg gggttggaat gcgttgcaag catggcttcc
     4321 gacaccgctt caggggcttg taatatgtta tcgtgaatgt agtggatttt actgggaaat
     4381 gcaaagtttt tctgtcgccc gccaagtcgg gaaactgcga aatgaaaaat aaaaatagtt
     4441 atttatctat atatatcaaa tttttaatag ataaaaaatc aaaattgttt atatattaat
     4501 ttttaaaaga ttgtcagcat attgcgttaa gt
//
LOCUS       DAXHTR010000148         4438 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.148, whole genome shotgun sequence.
ACCESSION   DAXHTR010000148 DAXHTR010000000
VERSION     DAXHTR010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.148"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(738..1550)
                     /gene="murI"
                     /locus_tag="ACFNML_001797"
     CDS             complement(738..1550)
                     /gene="murI"
                     /locus_tag="ACFNML_001797"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFC6393118.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALSTTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
     gene            1745..2206
                     /gene="tsaE"
                     /locus_tag="ACFNML_001798"
     CDS             1745..2206
                     /gene="tsaE"
                     /locus_tag="ACFNML_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFC6393119.1"
                     /translation="MFDFPPISRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHLGAVKSPTYAIVESYPLERFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHGGGGRKCLLTAHTERGRESLPL"
     gene            2203..3453
                     /locus_tag="ACFNML_001799"
     CDS             2203..3453
                     /locus_tag="ACFNML_001799"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HFC6393120.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            3533..4351
                     /locus_tag="ACFNML_001800"
     CDS             3533..4351
                     /locus_tag="ACFNML_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6393121.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTELSDRLPLSVLTDSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FSPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
BASE COUNT         1103 a         1286 c         1057 g          992 t
ORIGIN      
        1 tcattctaac cccctcttcc ccccccacat gactaacacg aaacagggat tttgacaccc
       61 gaaccgagac cccttgtatt tcccccgcga aaagccggca tccgcccgcg tatcatggga
      121 gcaacaaaac ccctgcctaa aattttgact tgtgcaaatt gggggtatat ttgggggtat
      181 attgaaaaat ggctaaaata aaatgtttaa taaacaaaat gttgaaactt aatttcgata
      241 gagcatctgc atatcgtatt gaggcgttca tggaatttga gaaagctatt tttaaataag
      301 aaaaggtaac tattaaatag ctacctttct aaaattaaat atcaacacat cgttaaaaca
      361 cagccaaaaa aaaaaccgaa accgaacggt ctggattccc gcctgcgcgg gaatgacggg
      421 tttcgagatt acagtgtatc gagaaggatg ggaaacggtg ggaattgtgt aaaaaatgcc
      481 gtctgaagcc tttcagacgg catttgcggc attcggacat ttaaaaatcc catcattccc
      541 gcgcaggagg gaatccgaat acatccgcac agaaacctgc atcccgtcat tcccacgaaa
      601 gtgggaatcc aggacgcaaa atctcaagaa accgttttac ccgataagtt tccgcaccga
      661 caggtctaga ttcccacttt cgtgggaatg acgggatttt aggtttctgt aatctcgggc
      721 agctttccgt catcgtttta acccaaagac accatttcaa tctgctccat cgtcctgccc
      781 agaaaacgct cgccgatggt tctgaatttc aaaggaatat cgctgacgta aaaacggtag
      841 tcgggattgt tgttgtcggt attgagcaat ccttcctgag caaggacgcg tgcggtttct
      901 tcggccgttg taattgcaga atcaaccaac gcgacattgc ccgcctccct gccgattaag
      961 ggcttgagca agggaaagtg cgtgcagccc aacaccagcg tatcgatgcc gtctgcaagc
     1021 aatggtttga ggtattcgca tacggtcagg cgggtaactt cgtgttccaa ccagccctct
     1081 tccaccaaag ggacgagcag cggcgcggcc tgcgtgcgga cgagcgtgtc ggggttgtcc
     1141 ctgtggatgg cgcgcgcata agcattgctg ttgactgtgg tattggtggc gataatgccg
     1201 attttgttgt tgcgcgtggt tgacagcgcg gctttcgcgc cggcggaaat cacgtccaac
     1261 acaggcatat tgcccgtctt ttgacggatt ttctgccccg caaccgccgc aatcgtgttg
     1321 cacgcgataa ccatcgcctt aacatcgtgt tccaataaaa aatcgacaat ctgcatcgag
     1381 aaattttcga tggtcgcctt agatttcgtc ccgtaaggca cgcgcgccgt gtcgccgaaa
     1441 taaatgatgt tctccatcgg cagccgctcc atcagcgcgc gcacattggt caaaccgccg
     1501 attcccgagt caaaaacgcc gatgggtcgc tgcctgccga tattttccat cctttttcca
     1561 atccgtccca aacatacaat ccgcgtattg tacacgcgcc gctttttctt gacagccatt
     1621 gccgtctgaa agcaggatgc ggatttggct gttataatgt acggcaatgc cgcatccggc
     1681 ccgccgggcg cacggtttgc cggccgccct ctccgatccg ccacataaaa gttttaaagc
     1741 ccctatgttt gatttccccc ccatttcccg attccttgcc gacgaagccg ccacactcga
     1801 tttgggcgcg gcgtggtctt cccgtttaaa cgcaccgctg gtcatttatc tcgaagggga
     1861 tttgggtgcg ggcaaaacca cgctgacacg cggcatccta cgcggattgg gtcatctggg
     1921 cgcagtcaaa agcccgacct acgccatcgt cgaatcttat ccgctggaac gcttcacctt
     1981 gcaccatttc gacctctacc gcttctcgtt ccccgaagaa tgggaagacg cggggcttga
     2041 cgaactgttt gccgcaaaca gcgtctgcct gatcgaatgg ccgcaacagg gcggggaatt
     2101 tacgccgccc gccgacatca ccgcaacatt gacacacggc ggcggcggca gaaaatgcct
     2161 gctgaccgcc cataccgaac gaggacgcga aagcctgccg ctatgaccaa actgacacga
     2221 agacaaatca tccgccgcac cgccggcaca ctgttcgccc taagccccat cgcatccgcc
     2281 gttgccaaaa cggtacgcgc cccgcaattc accgccgcac ggatatggcc gtcgcacacc
     2341 tacacccgcc tgacgctgga aagcaccgcc gcgctcaaat accagcactt cacgctcgac
     2401 aacccgggca ggctggtcgt cgacatacaa aacgcgaaca tcaataccgt attgcacgga
     2461 ctgtctcaga aggttatggc ggacgaccct tttatccgca gcatacgcgc gggtcagaac
     2521 acaccgacca ccgtccgcct cgtcatcgat ttgaaacagc cttcctacgc gcaagttttt
     2581 tccctccccc ccgtcggcgg ctttaaaaac cgcctcgtcg tcgatctcta tccgcaaggg
     2641 atggatcccg acgatccgat gatggcgctg cttaacggca gcctgaacaa aacccgtcgc
     2701 ggctcccctg aagccgacct cgctcaaaac actcccgcac aaaccgaacg cggcaaaaac
     2761 gggcgcagac ccgtcattat gatcgacccc ggacacggcg gcgaagaccc cggcgccgtc
     2821 agcccgggcg gcctacagga aaaacacgtc gtcctctcca tcgcccgcga aaccaaaaaa
     2881 caattggaag ccttgggtta caacgtcttt atgacgcgca acgaagacgt gttcatccca
     2941 ttgggcgtgc gtgtcgccaa agggcgagca cggcgggcgg acgtattcgt ctccatccac
     3001 gccgacgctt tcaccagccc ctccgcgcgc ggcacagggg tttatatgct gaacaccaaa
     3061 ggcgcgacca gctccgccgc caaattcttg gaacaaaccc aaaataatgc cgacgcggtc
     3121 ggcggcgtac cgaccagcgg caaccgcaat gtcgataccg ccctgctcga tatgacccaa
     3181 accgccacgc tgcgcgacag ccgcaaactc ggcaaactgg tgcttgaaga attgggcagg
     3241 ctcaaccatc tgcacaaagg cagggtggac gaagccaatt tcgccgtttt gcgcgcgccc
     3301 gatatgccgt ctatcctggt cgaaaccgcc ttcctgtcca atcctgccga agagaagctg
     3361 ctgggcagcg aatccttccg tcggcagtgc gcccaatcca ttgcctcggg tgtccaacgc
     3421 tacatcaata catccgtatt gaagcggggt tgattgaaaa ggcggtgttt caatatagtt
     3481 gtcggatgaa ggcaggcata aacctacttc cgccccaaac cgcgttttta tgatgaaagg
     3541 tatggataaa ttgagatacc aacaggattt tttaaatatc cgccccattt ttacagccgg
     3601 cgaacaagaa tacctgaccg aattgtccga tcggcttcct ttatctgttt taacggactc
     3661 cgtgcgtaat attgaagaaa tcggtatcga ttttgtgtac agttctgcca aattggaagg
     3721 aaacacttat aatcaatacg atacgcaggc attgcttaaa ctcgggcaaa ccgcaggcgg
     3781 gaagttatat tcggatgccg taatgttaat caacctgagg gaaagttacc ggcacctgct
     3841 gtccggtttg gacagccccg agccgtttga ctggttggat ttcttaaaaa ccacccacag
     3901 cctgatttcc gaaaacttac tggaaaaagg ctccggcggc gtagtccgcc gcgacagcgt
     3961 taccataagc gggacagact acaccccttt atctaacccg caaagtttgg atacggaatt
     4021 aaaatggttg cttcaagaag ctcccaaaat agaaaatcct ttcgaccgcg ccgtttatct
     4081 ccataataat ttggcgtatc ttcaatattt taaagattgc aataaacgta ctgccagaaa
     4141 ctgcatgacc ttgtcgctga tgcgctccgg tttttttcct tgcgtattct cccccgacag
     4201 ttatcccgcc tatgccgagg ctgtcgtagc ctattacgaa accggcgatt acggtttatt
     4261 caagaaatat tttatttcgg catacgaaaa taccgtaaat aagtacggcc cacagcctga
     4321 tatggatatt tcccgcaact tctcccttta attccaaggg gttgttatcc gtatcttccg
     4381 cacgcggcaa atgcggcggc acagtggaca cacccctccc ttaccttcgg ttatagtg
//
LOCUS       DAXHTR010000149         4386 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.149, whole genome shotgun sequence.
ACCESSION   DAXHTR010000149 DAXHTR010000000
VERSION     DAXHTR010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.149"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(46..3078)
                     /locus_tag="ACFNML_001801"
     CDS             complement(46..3078)
                     /locus_tag="ACFNML_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFC6393122.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPNASDGYSDSGNGTEEAETEAAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNGAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSRMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFRRHAGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEARISR
                     LIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIYIEEPAAPDAWVVE
                     PPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPAEFEQAQRSRIAETDHLADDVLNGGWQ
                     EETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSCRVSDTEADE
                     GAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVKVKVV
                     DSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGLELPN
                     PKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTGSGKS
                     VGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANALNWC
                     VNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLPFIVV
                     VVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANIPTRI
                     AFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRVVEYL
                     KQFGEPDYVDDILSGSGSEELPGIGRSGDDETDPMYDEAVSVVLKTRKASISGVQRAL
                     RIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3243..3923
                     /locus_tag="ACFNML_001802"
     CDS             3243..3923
                     /locus_tag="ACFNML_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HFC6393123.1"
                     /translation="MIFQTVWFSDVVLSVSWIVLILILAASAPSAFRSLARYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAAFWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALAVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRGIWR"
     gene            3952..4311
                     /locus_tag="ACFNML_001803"
     CDS             3952..4311
                     /locus_tag="ACFNML_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFC6393124.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETINHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALANIFLHTA
                     GSLLLTWLGLKIGAAVK"
BASE COUNT          888 a         1288 c         1117 g         1093 t
ORIGIN      
        1 tgccgtctga aacagtcttc agacggcatt tccatttgca aaaaatcaag cattgtccaa
       61 ggggacgaga atcgtacggt tgccgttgtg ttccggtgcg gacacaatgc cttccgcctc
      121 catctggtca atcagacgcg cggcgcggtt gtagccgatg cgcaaggcgc gctgtacgcc
      181 cgaaatactg gctttgcgcg ttttcaggac aacggatacg gcttcatcat acatcggatc
      241 ggtttcgtcg tcgccgctgc gcccgatgcc gggcagctct tcgctgccgc tgccgctcaa
      301 aatatcgtca acatagtccg gctcgccaaa ctgtttcaaa tattcgacca cgcggtgcac
      361 ctcttcatcc gaggcaaacg cgccgtgaac gcgctgcgga taggcagtac ccggcggcag
      421 gaacagcata tcgccctgac cgagcaggtt ttccgcgccc atttggtcga gaatcgtgcg
      481 gctgtcgatt ttgctggaca cttggaacgc gatacgcgtc gggatgttcg ccttaatcag
      541 acccgtgatg acatcgacgc tggggcgttg tgtggcaaga atcaaatgga tgcctgccgc
      601 gcgggctttt tgtgcgaggc gcgcaatcag ttcctcgatt ttcttgcctg ccgtcatcat
      661 caaatcggca aactcatcga ccacgaccac gataaacggc agtttttcca aaggttcggg
      721 atcgtcgggc gtgaggctga acggattgcc gattttttct ccccttgctg cggcttcggc
      781 gattttttga ttgaagcccg caagattacg cacacccata aagctcatca ggcggtagcg
      841 tttttccatt tcgttaacac accagttcag cgcgtttgcc gccagcttca tatcggtaac
      901 gacaggggcg agcaggtgcg ggatgccttc gtaaatgctc aattccagca ttttcggatc
      961 gatcataatc atacgcacgt cttccggcgc ggctttgaaa agcatagaca gaatcatcgc
     1021 gttgacaccc accgatttgc ccgaaccggt cgtgccggca accaacaaat gcggcgcttt
     1081 tcccaagtcg gttacgacgg gctgtccggt gatgtcctga ccgagcgcga gcgtcagctt
     1141 ggatttggat tcggcaaact cgggcgaatt gaagatttcg ctcaggcgta tcatttggcg
     1201 tttcgggttc ggaagttcca aacccataca ggttttgccg gggatggttt cgacaacgcg
     1261 gatggaagcc acgccgagcg aacgcgccaa gtctttttcc agattcagaa cggaattgcc
     1321 gcgcacgccg acatcgggtt cgatttcata acgcgtaatc acggggccgg aataagaatc
     1381 gacaaccttg accttgactt tgaactccgc caatttttct tcgatggtga tgctgttttc
     1441 caacagttct tcttcggttt gcgtcgcctc gggattgaac agcggcggca gaagcaggtc
     1501 ggttgtcggc aggtgttcgg ataccgcacc ggtctcttcc gattggaacg ccccttcatc
     1561 cgcttccgta tccgataccc ggcaggacgg gcgttcagac ggcacatttt caaacggaca
     1621 aaccgcctga ctgtcatgcc cgaacgcttc ggtttccgac agatattgcc cgcttgaccg
     1681 ctctgccgca ccttcactgc cgtcatctgc aatagcggcg gtttcctcct gccaacctcc
     1741 attcaaaaca tcatcggcaa gatggtcggt ttcggcaatg cggctgcgtt gcgcctgctc
     1801 gaattctgcc ggcggttcat aggtacggtt gtagatttcc gatacgggag gcggcggcgg
     1861 aatatcgatt gcgggcatgg gaactttcgg cacttcgggt ggttcgacca cccaagcatc
     1921 gggcgcagca ggttcttcaa tatagatatc ggcagcttca tcataaaccg gaccgccgta
     1981 tcccacagac gaaacggttt ccgtgaaaac attttcggtt tcagacggca tttcgacatc
     2041 ccgtttcccg acaaccgtcc gactttccgg aatcaggcgc gaaatccgcg cctccgcaga
     2101 aaccgttgcc ttgttcggtt ctttcaaatt gacggaaacg cggcggcagg catcgcggac
     2161 ggctgtgccg tctgaaacgg actgtccttg ggaaacatcc ggggattttg cctccgcctg
     2221 ccctgcatga cggcggaacg gcgcattccg tgcattttcg gctttgactg cagaaaaggc
     2281 agacggatgc tgcttctcaa aatacggctc aaatccgtaa tccgcagact cggaaaacgc
     2341 ttctttgtcc gcatcgaaca tacgggaata cgtgcgttga agcacagggt cgtccaaacc
     2401 gataatctgc agcccttcca tcggggtatc ggacacgcgg actttgggga ccgctgtttc
     2461 gtttttctca aatgcatcga tatagcgttt tttcgtttcc cttaaagccg cgccgtttaa
     2521 ggcacgcgtt gcttcttcca aagtgatttc cttaaaaacc ggacggacgg gcgaaatttc
     2581 gctttcagac ggcatcaacc cttcagcaat actccggtcg aatgggatgc ggcggttgtc
     2641 gattacggca gttgcaatgt cttccgtatc ggcagcctct tcctccgcag cttctgctgc
     2701 ttccgtttcc gcttcttccg tcccgtttcc actgtctgaa tatccgtcgg atgcgttcgg
     2761 aacatccgca cggtttgcag aagcaggcgg aacaggccta tccggttcgg attcgtgcgc
     2821 cagatagttt cggcagaaac ggacgacacc gacgaacagc caatacactg ccgttttgac
     2881 ggcatggaaa agcatcaggg caagttcggg aaaatcgggc ataccgtctt tgatttcagg
     2941 cagctccgcc tgtttttccc ctttcttttc ctgccacgca gaaacctcgc gcatccactc
     3001 gcgttcggat tgtgcgcgga caaaaaacaa gccggcaagc gcaagcaaca aaataacgat
     3061 cataactatc caaaacatat gccgtttccc gcaagacaaa cgattaaacg ggtttatttt
     3121 aacgtgtttc catcccaaaa aaaacaaccg gtcggcaatt ctgccgaaac cgattcggct
     3181 ttcggtttgc ccccaccgac agtataatag ccgtcccttc ccttaatctt tgcggcattg
     3241 ccatgatttt ccaaacagtc tggttttcag atgtggtact gtcggtttcg tggattgtcc
     3301 ttatcctgat actggcagct tccgctccgt cggcattccg ctcgcttgcc cgctaccgtt
     3361 ctgcccttcc cctgtgtacc gtaattttct ccgcggcatg gtgtctgaac gcctctgccg
     3421 gcggcggaca gcttgcacag atgaactacc acctgctcgc cgtcaatcta gttgctctga
     3481 tggtgggcac ttccgccgcc ttttggcttg ccgcgctgct gatgctgccc tactgcctgc
     3541 tgtttgccgg ttctgtcggc gcatatccgc cgaacgcttt ggtgctgatt ctgcccgcgc
     3601 tggccgtcaa ccgcctgtcg cgtatgctgg tcaaccgcct gccacccaat attttcatct
     3661 ttattttcgt caacggcttt ctcgcttccg ccgccggcat tctgctgacc gggctggtgc
     3721 tgaccggtat tttggatgcc gcaaacgctt ttccgtccga aatattgtgg acgaccgccc
     3781 tgcccgtctt tattctgctg gcgtgggcgg aagcttttct cagcggcatt tcaactgcca
     3841 tatttgttgc actgaagccc cattggatca acactttcga cgacaaccgc tatctgaagt
     3901 ccgaccgcgg catatggcgg tagccttcag acggcataga caggcaaaac catgctttcc
     3961 aacatcatcc ccctttccat cggtgcggca ctcggtgcaa cggcgcgttg gctgctcaac
     4021 ctcgccgttc ctgccgccat gtctcccgcc acgggcaacc tgtttgccaa ctggacgggc
     4081 gcgctgctca tcggcatctt tgccgaaacc atcaatcacc cgcaatggaa gctgctgctg
     4141 attaccggtt ttctcggcag cctgaccacg ctttccggat tttcactgga aaccgtaacc
     4201 ctgctccaat cgaatcgtcc tgcttcagca cttgccaata ttttcctaca cacggcaggt
     4261 tcgctgctgc tgacttggct cgggctgaag ataggggcgg cagtcaaata accgcaacaa
     4321 attgcctccc tttgttttga cataatctat aaattagctt cgcttgttta aatactggct
     4381 gtgcca
//
LOCUS       DAXHTR010000150         4373 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.150, whole genome shotgun sequence.
ACCESSION   DAXHTR010000150 DAXHTR010000000
VERSION     DAXHTR010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.150"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(69..2045)
                     /locus_tag="ACFNML_001804"
     CDS             complement(69..2045)
                     /locus_tag="ACFNML_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HFC6393125.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVLTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGENGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLKGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="ACFNML_001805"
     CDS             2194..3033
                     /locus_tag="ACFNML_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HFC6393126.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALAQIDETACIGCTACIRACPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="ACFNML_001806"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="ACFNML_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFC6393127.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKVHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKDKLDGLAIRVPTVNVSLVDLNFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMIKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
BASE COUNT         1115 a         1456 c         1011 g          791 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgcctttc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg acggacggtt
      301 tcgccgtttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg tcagcaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcgagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gctgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccaa ccgccgatca atgcctgaat gtccagcttc
     1201 atgccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatgc cggcaacgaa tgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacgggacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaacac cagccgcctc
     1801 attgccctca gcttgggcag cgaaaactcc aagcctatac tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgttgcatga cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggtc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc agatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgcctgc
     2461 cctgccgatg ccattatggg tgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgtgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgcaacgc gcgtccgcaa
     2761 acacccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat atctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacagaa taccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gccgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 tcaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaagttcatc tggaaagcgg cgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 tgaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ctggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaagacaaa ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgaacttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacactga
     4141 ccaaagtcgt tgacggcaac atgattaaag tcttcgcatg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccgctca
     4261 aataagcaat aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttt catttttggc agtgattttg cccggctgtc gtcatttcta att
//
LOCUS       DAXHTR010000151         4312 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.151, whole genome shotgun sequence.
ACCESSION   DAXHTR010000151 DAXHTR010000000
VERSION     DAXHTR010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.151"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            501..2018
                     /locus_tag="ACFNML_001807"
     CDS             501..2018
                     /locus_tag="ACFNML_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="surface lipoprotein assembly modifier"
                     /protein_id="HFC6393128.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALLQVQRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRELHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAAKLDLPAPVLENVGRFRKKTEGLTGWRFSGGISPAVNRNANNAAPQYCWQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLPPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGIAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
     gene            complement(2077..3957)
                     /locus_tag="ACFNML_001808"
     CDS             complement(2077..3957)
                     /locus_tag="ACFNML_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HFC6393129.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFGPEELDALDGALQKGWQ
                     KGLHSVLFADYEFGLPLTGVESERGGNLALHWFADCADTDAASWLARHSDDLPAGIST
                     PQSSVSETDYLDRIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDAEGQSAWTLCFSPELFLNIASDGTISTEPMKGTAPILGDGQDERRAAELQAD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLKPCAGGLGFEGIFNV
                     VIRTLSLKPVSASDGIVSGIGGPDSNAQARTAGQGGATPHPFDSNPPYRGVYGVGSGI
                     VIDSDPAAEYRECGWKARFLNELRPDFGIFETLRAENGRCTLLDRHLCRLKTSAQALN
                     LPLPDGCENQIKQYIADLPDGAFRVKALLASDGISLSRAVLNRLTDKQRVIISPTILP
                     AQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKYQGQWLT
                     PSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFAAA
                     LA"
BASE COUNT          883 a          966 c         1407 g         1056 t
ORIGIN      
        1 ttttctgtcc ttgtgggaat gacgggatgt aggttcgtag gaatgacgtg gtgcaggttt
       61 ccgtgcggat ggattcgtca ttcccgcgca ggcgggaatc tagtctgttc ggtttcagtt
      121 atttccgata aatgcttgtt gcttttcatt tctagattcc cactttcgtg ggaatgacgg
      181 cgacagggtt gctgttatag tggattaaca aaaaccggta cggcgttgcc tcgccttagc
      241 tcaaagagaa caattctcta aggtgctgaa gcaccgagtg aatcggtttc gtactatttg
      301 tactgtctgc ggcttcgtcg ccttgtcctg atttttgtta atccgctata aatgtgaaat
      361 ccgccctttg aaaatcgggc ggcggttttt tgcctgcccg ctttcagacg gcaaagccgg
      421 ttttcacggg tttctgcctg tttttcggat ggtttgacgt gcttcggcgg cgtgtttgcc
      481 agaaaggtaa atgacagggt atgttgtatt tcagatacgg ctttttggtt gtttggtgtg
      541 cggcaggtgt ttctgccgcc tatggggcgg atgcgcctgc gattttggat gacaaggctt
      601 tgttgcaggt gcagcggtcg gtgtcggata agtgggcgga atcggattgg aaagttgaaa
      661 atgatgcccc gcgcgtggtt gacggggatt ttttgttggc gcatccgaaa atgttggaac
      721 atagtttgcg cgacgcgctc aacggcaatc aggcggattt gattgcttcg ttggcggatt
      781 tgtatgccaa gctgccggat tatgacgcgg ttttgtacgg cagggcgcgg gctttgctgg
      841 cgaaattggc gggaaggccg gcggaggcgg tggcgcggta tcgggaactg cacggggaaa
      901 atgcggcaga cgagcggatt ttgctggatt tggcggcggc ggagtttgac gatttccggc
      961 tgaagtcggc agaaaggcat tttgcggagg cggcaaaatt agatttgccg gcgccggttt
     1021 tggaaaatgt ggggcgtttc cggaaaaaaa cggaggggct gacgggctgg cgtttttcgg
     1081 gcggcatcag cccggcggtc aatagaaatg ccaataatgc cgcgccgcag tattgctggc
     1141 aaaacggagg ccggcagata tgcagtgtca gccgggcgga gcgggcggca gggttgaatt
     1201 atgaaattga ggcggaaaag ctgacggcgt tggcggacaa tcattatttg ttgttccgtt
     1261 ccaatatcgg cggcacgagc tattatttca gtaaaaaatc agcttatgat gacgggttcg
     1321 gcagggcgta tttgggttgg cagtataaaa atgcgcggca gacggcgggg attttgccgt
     1381 tttatcaggt gcagttgtcg ggcagcgacg gctttgatgc gaaaacaaaa cgggtaaaca
     1441 accgccgcct gccgccgtat atgctggcgc acggagtcgg cgtgcagttg tcccatactt
     1501 accgcccaaa cccgggatgg caattttcgg tcgcgctgga acattaccgc caacgctacc
     1561 gcgaacagga tagggcggaa tacaataacg gcaggcagga cgggttttat gtttcgtcgg
     1621 caaaacgttt gggcgaatcg gcaactgtgt tcggcggctg gcagtttgtg cggtttgtgc
     1681 cgaaacgcga aacggtgggc ggcgcggtca ataatgccgc ctaccggcgc aacggtgttt
     1741 atgccggttg ggcgcaggag tggcggcagt tgggcggttt gaacagtcgg gtttctgcgt
     1801 cttatgcccg ccgcaactat aagggcattg cggctttctc gacagaggcg caacgcaacc
     1861 gcgaatggaa tgtctcgctg gctttgagcc acgacaagtt gtcgtacaaa ggtatcgtgc
     1921 cggcgttgaa ttatcgtttc ggcaggacgg aaagtaatgt gccgtatgcg aaacgccgca
     1981 acagcgaggt gtttgtgtcg gcggattggc ggttttgaat ggtgggataa tgccgtccga
     2041 actttggaaa caggttcgga cggcattttt gcgcgttcag gcaagggcgg cggcaaatac
     2101 gccgcgcagg gcgttggaga ggcggatttc ttcggcttct tgcagtgttt tttgtgtgat
     2161 gtgtgtttcg attacttgat ttgtttgcaa atatttttgc ggttcgtcca atacggcttg
     2221 gcgcattatg ccgtttaaaa tgtctaaatc taaagagggc gtaagccatt gtccttggta
     2281 tttgacgaac acgttgcttc tgccgccttc gagcaggatg ccgtctgaat tgaaaaacag
     2341 gctgtcgaac gcgccttgtg tttcggcggt ttgccacgct tggtcgaaga cggtgcggca
     2401 ggtggttttg aagcggcgca ggtagttttg tgcgggcagg atggtcggcg aaatgatgac
     2461 gcgttgtttg tcggtcagac ggtttaaaac ggcgcgggac aggctgatgc cgtctgaagc
     2521 gagcagggct ttgacgcgga acgcgccatc gggcaagtcg gcaatgtatt gtttgatttg
     2581 attttcgcag ccgtcgggca ggggcaggtt gagggcttgg gcggaggttt tcagacggca
     2641 taggtggcgg tcgagcaggg tgcagcgtcc gttttccgcg cgcagggttt caaaaatgcc
     2701 gaagtcgggg cgcaattcgt tgaggaaacg ggctttccag ccgcattcgc gatattcggc
     2761 ggcggggtcg ctgtcgatga cgatgccgga accgacaccg tacacgcccc gatacggcgg
     2821 attggagtca aacggatgcg gcgttgcccc gccttgccct gccgttcggg cttgcgcgtt
     2881 tgaatccggg ccgccgatgc ctgaaacaat gccgtctgaa gccgaaacgg gtttgagcga
     2941 caaggtgcgg atgacgacgt tgaatatgcc ttcaaacccc aacccgcccg cgcaaggttt
     3001 caggtagccg atgctgcccg tataaagccc acgcgcttcg gtttcgagcg attcgataat
     3061 ctgcatactc atttttttgg gcgcgccggt gatgctgccg caggggaagg cggcgcggag
     3121 gatgtcggcg aacgaggtgt ttggcagggc ttgggcttgg atggtgctgg tcatctgcca
     3181 aacgctgccg aaacgcgaca ctttaaacgg ctcgggcacg catactttgc cggtttgggc
     3241 gattttgccg aggtcgttgc gcagcaaatc gacaatcatc acgttttcgg cgcggttttt
     3301 cgggtcggct tgcaattcgg cggcgcggcg ttcgtcttgt ccgtcgccca aaatcggcgc
     3361 ggtgcctttc atcggttcgg tgctgatggt gccgtctgaa gcgatgttga ggaagagttc
     3421 gggcgagaaa cacagcgtcc acgcggattg cccctccgca tcgggcaggt gggacaagac
     3481 ggcatagggg acgggctggc gcaggcggcg gtagaggctg acgggattgc cgtaggcttg
     3541 caggtgcagg cgggtggtgt agttgatttg ataggtgtcg ccgcgccgga tggcttcgtg
     3601 gatttggcgg atgcggtcga ggtaatcggt ttcggatacg gaggattgcg gcgtggaaat
     3661 gccggcggga aggtcgtctg agtgtcgggc aagccagctt gcggcatcgg tgtcggcgca
     3721 gtcggcaaac cagtgcaggg caaggttgcc gccgcgctcg gactcaaccc ccgtcagcgg
     3781 caaaccgaat tcgtagtctg caaacaacac cgaatgcaac cctttttgcc agcccttttg
     3841 cagcgcgccg tccaaagcat cgagttcttc gggaccgaaa aaacggcttt ctacatgatt
     3901 ttgatagcgt tttgcgcggc cgcttacggc atcgtcaaac agggcgaaat agggcatggc
     3961 aattcggggc aaatgttttg attatacgct ctttttacac atattttcag acgattcggc
     4021 aaatatcggc aaaatgtaat tttatgtaga gaaagtgggg ggcgaaggtg taaaattgtg
     4081 aaaacaaatt ccgatttcca acctgaaata caataaggag acctttatga cagaccacca
     4141 gctgcaaccg tttgaaaacg tagaattagg cgaaaagcaa gaccagctcc aagtatagtg
     4201 gattaacaaa aaccagtaca gcgttgcctc gctttagctc aaagagaacg attctctaag
     4261 gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctgcgg ct
//
LOCUS       DAXHTR010000152         4275 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.152, whole genome shotgun sequence.
ACCESSION   DAXHTR010000152 DAXHTR010000000
VERSION     DAXHTR010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.152"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..183
                     /locus_tag="ACFNML_001809"
     CDS             <1..183
                     /locus_tag="ACFNML_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393130.1"
                     /translation="KERFSLMLHQVWGDVSTHRIAPDARFMRLARSAAGNLPAFPASG
                     GRNGRHTVYKPQYRLQ"
     gene            216..1136
                     /gene="ribF"
                     /locus_tag="ACFNML_001810"
     CDS             216..1136
                     /gene="ribF"
                     /locus_tag="ACFNML_001810"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFC6393131.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADT
                     RGLPVVTVVFEPQPKEFFALRTGRMPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSGCSQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMAELKRQIAADMEAAKCW"
     gene            1277..4066
                     /gene="ileS"
                     /locus_tag="ACFNML_001811"
     CDS             1277..4066
                     /gene="ileS"
                     /locus_tag="ACFNML_001811"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFC6393132.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQVARQKKDFIRLGVLGDWDRPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKISPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSAGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGISILAETTGDKLENLHMNHPFLERDIPMLNGEHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGKYIGETLRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCEHYDKLPDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKDIVSFCSEDLGAFYLDILKDRLY
                     TTKADSHARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKAEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMASYL
                     NALGEELRFALLVSKAEVKVGDELAVAAKASDGEKCERCWHYTHDVGAVAGHETICKR
                     CAGNVGGEGETRHYA"
BASE COUNT         1053 a         1179 c         1131 g          912 t
ORIGIN      
        1 aaagaacgat tttcactgat gttgcatcag gtttggggcg atgtttcaac acataggatc
       61 gcgcctgatg cacgctttat gcgtttggcg cgttcggcgg cgggaaattt gcctgctttt
      121 ccagcgtcgg gcgggcgtaa cgggcggcac actgtctata aaccgcaata ccgtttacaa
      181 tgaccgcctg tttcaccaca tacccgaacg caacaatgag aatcaggctg gggcggcaca
      241 acgcgcccga ctttccgcac ggggccgccg taaccatagg caatttcgac ggcgtacacc
      301 ttggacacaa acacatcctc caaaaactcc gcctcgaagc cgatacgcgc ggattacccg
      361 tcgtgaccgt cgttttcgaa ccccaaccca aagaattttt cgcactccgc accggcagga
      421 tgccaccgtg tcggatcagc cccctgcgca ccaagctcga attattggaa ggcacaggct
      481 gtgtcgatgc cgtctgggtt ttgcgtttcg atcaaaattt ttccgaaata tccgcgcaag
      541 ggtttatcga ccgcctgctg cgtcaaacct tgaatacgcg ttatttgctc gtcggcgatg
      601 atttccgttt cggtgcgggg cgggaaggct gttttgaact tttggcacaa cagcccgata
      661 tgcagaccga gcgtacgcct tccgtcatcg tcgaagacat ccgcaccagc agtaccgccg
      721 tgcgacaagc cctttcagac ggcaaccttg cctatgcgaa aaaacttttg ggacacgact
      781 acgtcttgag cggcagggtg gtgcacggca gaaaactcgg acgcacctta aacgccccga
      841 ctgccaacat ccgcctgccc cgccaccgtt atgcactcgg cggcgtgttc gtcgtcgaag
      901 cggacggcgc attcggcacg cggcgcggcg tggcgagctt cggtttcaat cccaccgttg
      961 atagcggctg ttctcaaaag cttgaagtcc acctgttcga ctttcaaggc gacctgtacg
     1021 gacaacggct gaacgtccgc ttcctgcaca aactgcgcga tgaagaaaag tttgacggta
     1081 tggcagaact gaaaaggcag atagcagccg atatggaagc cgcaaagtgt tggtagaaaa
     1141 accttataca aaccatccga ttgggctaca atcaatcttt taactgttca gacggcatag
     1201 gggtttcccg ttgtgaaata ctgtttgagg cgcaatgccg tctgaaccga aatattgtaa
     1261 caatagagat taaaaaatga ccgattacag caaaaccgtc aacctgctcg aaagcccgtt
     1321 cccgatgcgc ggcaatcttg ccaagcgcga gcctgcatgg ctgaaaagct ggtacgagca
     1381 aaaacgctac caaaaactgc gcgaaatcgc caaaggccgt ccgaaattca tcctgcacga
     1441 cggcccgccg tatgccaacg gcgacatcca catcggtcat gccgtcaata aaattctcaa
     1501 agacatcatt atccgcagca aaacccaagc cggttttgac gcgccttatg tgccgggttg
     1561 ggactgccac ggcctgccca tcgaagtgat ggtagaaaaa ctgcacggca aagacatgcc
     1621 caaagcgcgt ttccgcgaat tgtgccgcga atatgccgcc gaacaagttg cccgccagaa
     1681 aaaagacttt atccgcttgg gcgtgttggg cgattgggac cgtccttacc tgaccatgga
     1741 tttcaaaacc gaagccgata ccgtgcgtat gctcggcgaa atctacaaat ccggctatct
     1801 ctaccggggt gcgaaaccgg ttcaattctg cttggactgc ggttcttcgc tggccgaagc
     1861 ggaagtggaa tataaagaca aaatctcgcc cgcgattgac gttgcctatc cgtttaaaga
     1921 caccgccgcg cttgccgccg cattcggttt ggcaggtatc gaaggcaaag cgtttgccgt
     1981 tatttggaca accacgcctt ggactttgcc tgcgagccaa gctgtttcag cgggtgcaga
     2041 cgtggtgtat caattaatcg atacgcccaa aggcaaattg gtattggcca aagatttggc
     2101 ggaagacgct ttgaaacgct acggcttttc agacggcatt tctattcttg ccgaaaccac
     2161 cggcgacaag ctggaaaacc tgcacatgaa ccatccgttc ctcgaacgcg atattcccat
     2221 gcttaacggc gaacacgtta ccaccgatgc cggtaccggc ttggtgcata ctgcgcccgc
     2281 gcacggtttg gaagactacg ccgtctgcaa caaatacggc atcgagcttt acaatcctgt
     2341 caacgccgaa ggcaaataca tcggcgaaac gctgcgcgtt gccggtatgc gcgtttggga
     2401 ggcgaacccc gtcatcctgc aatggttgga agaaaccggc aaccttttgg caagcagtaa
     2461 aatcgaacac agctacgccc actgctggcg gcacaaaacg ccgctgattt accgcgcgac
     2521 cggtcaatgg tttgtcggta tggacaaagc cggtgccgac ggcaaaaccc tgcgcgacaa
     2581 agccatcaag gccgtggacg acaccgaatt cttcccgtct tggggtcgcg cgcgtttgga
     2641 agccatgatt gaaggtcgtc ctgactgggt ggtttcacgc caacgctatt ggggcacgcc
     2701 gatgactttc tttgttcaca aagaaacggg cgagctgcat ccgaactctg ccgaactttt
     2761 ggaaaaagtt gccctgaaaa tcgaagaaaa aggcatcgag gcttggttct ccctcgataa
     2821 aagcgaatta ttaagcgcgg aagattgcga acattacgac aaactccccg atacgatgga
     2881 cgtatggttc gattccggtt cgacccatta ttccgttgtg aaacaacgcg aagaattgga
     2941 atggccggcg gatttgtatc tcgaaggcag cgaccagcac cggggctggt ttcaatcgtc
     3001 catgctgacc ggctgcgcct catcaatggg acgcgcaccg tataaacagc tgctgaccca
     3061 cggttttgtt gtagacggcg aaggcaaaaa aatgtcgaaa tccatcggca acgtcgtcgc
     3121 gccgcaagaa gtctataacg aattcggcgc ggacatcctg cgtctgtggg cggcatctac
     3181 cgattacagc ggcgaattgg cgatttctaa agaaatcctt aagcgcgtaa ccgaaagcta
     3241 ccgccgtatc cgcaatacct tgagcttttt gtttgccaac ttgagcgact ttaatccgat
     3301 tgaagatgcc gtgcaacagg cggatatggt ggaaatcgac cgctatgccc tggtgttggc
     3361 gcgtcggctg caagagcgtc tggcaggcga ttactatccg cgctacgcct tccacttcgc
     3421 cgtaaaagac attgtttctt tctgctcgga agacttgggc gcgttctacc tcgacatcct
     3481 gaaagaccgc ctctatacca ccaaagcaga cagccatgca cgccgcagcg cacaaactgc
     3541 cctgtatcac atcacacgca gtttggttct cttgattgca ccgattttgt gcttcaccgg
     3601 cgaggaagcg tgggacatca tcggcggcgg cgaagaagac agcgtccttt tccatacttg
     3661 gcacgagttc ccgaccatca acgaaaaagc cgaagccgaa ctggtgaaaa aatggacggc
     3721 aatccgcgaa gcccgcgaag cggtaaccgc cgccattgag cctttgcgcg ccgacaaaac
     3781 cgttggttcg tccttgcagg cggaagccga aatcactgcg ccggaagaaa tggccagcta
     3841 cctgaacgct ttgggcgaag aattgcgctt tgccctgctg gtgtctaaag ccgaagtgaa
     3901 agtaggtgat gaacttgccg ttgccgccaa agccagcgat ggtgaaaaat gcgaacgctg
     3961 ttggcactac acccacgatg tgggcgcagt tgcaggccat gaaaccatct gcaaacgctg
     4021 cgccgggaat gtcggcggag aaggcgaaac gcgccattac gcctgataaa gtttgagcaa
     4081 atgccgtctg aaaccgccaa acagcatttc agacggcatt ttttgtaccg cgatttgtct
     4141 ttataacggc ggaggggttt caagattgcg gtgttgtcgg aatgcaactg aaccgtcatt
     4201 cccacgaaag tgggaatcta gaatctcgag gtttcagtca tttccgatag attcccgcct
     4261 gtgcgggaat gacgg
//
LOCUS       DAXHTR010000153         4198 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.153, whole genome shotgun sequence.
ACCESSION   DAXHTR010000153 DAXHTR010000000
VERSION     DAXHTR010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.153"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..561
                     /locus_tag="ACFNML_001812"
     CDS             <1..561
                     /locus_tag="ACFNML_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HFC6393133.1"
                     /translation="LAFRYSMLHLKDKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSH
                     RAIDFSKTPEAIFRCNLHTELKKKRKLALRLGKLSDNTAWILKPQVMKNLLKNPSTQI
                     TENDVVLDVKQKGVDMRIGLDISSITLKKQADKIILFSGDSDFVPAAKLARREGIDFI
                     LDPMWNHVPPDLMEHIDGLKTKCPKP"
     gene            732..1427
                     /locus_tag="ACFNML_001813"
     CDS             732..1427
                     /locus_tag="ACFNML_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HFC6393134.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSVQSSSSAPAAPVE
                     LNEGVNYTVLSTPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(1758..3128)
                     /gene="ffh"
                     /locus_tag="ACFNML_001814"
     CDS             complement(1758..3128)
                     /gene="ffh"
                     /locus_tag="ACFNML_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFC6393135.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKTLTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
     gene            3349..4155
                     /locus_tag="ACFNML_001815"
     CDS             3349..4155
                     /locus_tag="ACFNML_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFC6393136.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYPLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
BASE COUNT         1060 a         1011 c         1002 g         1125 t
ORIGIN      
        1 ttggctttcc gatattcgat gctgcatctg aaagataaaa tcaacggcac agatcaaatg
       61 gatgatttat atagaatttt cttttacgac tgcgtgccgt ttgaaaagaa aatgcacaat
      121 cccgtatctc atcgtgccat agatttttca aagactccgg aagccatatt tcgttgcaat
      181 ctgcataccg aattgaagaa gaagcgtaaa ttagcgttac gtttaggcaa gctgtcggac
      241 aatacagcat ggatattaaa accccaagtc atgaaaaatc ttctgaaaaa cccgtcaact
      301 caaattacgg aaaacgatgt cgtgctcgat gttaaacaaa aaggtgtaga tatgcgtata
      361 ggcttggata tttcatctat taccttaaaa aaacaagccg ataaaatcat cttgttttct
      421 ggtgattccg attttgtccc agcagccaaa ttagccagac gggaaggtat cgattttatt
      481 cttgatccta tgtggaatca tgtcccacct gacttaatgg aacacatcga cgggttgaag
      541 acaaaatgtc cgaaaccgta aattactaga acaaaggccg tctgaaaata aggctaatta
      601 tctaagccat atttcagacg gcctttataa aggctgaaat aaaactaaac acattttttc
      661 actcaaatcg aacacgcctt caataaaatg ccataaaatc cgcacattaa tctgacacac
      721 aagagatacc tatgaaactg aaaaccttag ctttgacttc attgaccctg ttggcattgg
      781 ccgcttgtag caaacaggct gaaaccagcg ttccggcaga cagcgtccaa agcagctcat
      841 ctgctccggc agcccctgtt gagttgaacg aaggtgtgaa ctacactgta ttgtctacgc
      901 ctattccgca acagcaggcc ggtaaaatcg aagtattgga atttttcggc tacttctgcc
      961 cgcattgcgc ccatcttgag ccggtcttga gcgagcacat caaaacgttt aaagacgata
     1021 cctatatgcg ccgggagcat gtcgtgtggg gtgatgaaat gaaacctttg gcacgtttgg
     1081 cggccgcagt ggaaatggcc ggtgaatcag ataaagccaa cagccatatt ttcgatgcga
     1141 tggttaatca aaaaatcaat ctggccgata ccgataccct gaaaaaatgg ctgtccgagc
     1201 aaacagcgtt tgacggcaaa aaagtattgg ctgcatttga ggctcctgaa agccaagcgc
     1261 gtgcggctca aatggaagag ttgaccaata aattccaaat cagcggcaca ccgactgtga
     1321 ttgtcggcgg caaataccaa gttgaattta aagactggca gtccggtatg accacgattg
     1381 accagttggt ggataaagta cgcgaagagc agaaaaagcc gcaataagtt gaggattgaa
     1441 tgagtaaagg ccatctgaaa ataggatttc agacggcctt ttgtatttag gctttataga
     1501 agagatgatt gcttaaagcc ttatggtttt aaatcagaat atatagcgga ttaacaaaaa
     1561 ccagtacggc gttggctcgc cttagctcaa agagaacgat tctctaaggt gctgaagcac
     1621 caagtgaatc ggttccgtac tatctgtact gtctgcggct cgccgccttg tcctgatttt
     1681 tgttaatcca ctataaatca gaatataaaa caaaaacgcc gtctgaaatt ttagacggcg
     1741 ttttcggtca aatcggctta caaacccggg aacattcctt tcattccctt cattcctttc
     1801 gccatacgca tcagtttgcc caagccgttg ccgctgaaca tcttcatcat ttgttgcatt
     1861 tgttcaaact gtttgagcaa tttgttcact tcctgtacgg tcgtgcccgc acccattgca
     1921 atacggcgtt tgcggctggc tttgagcagg gcagggttgg cgcgttcttt tggagtcatc
     1981 gagttgatga tggcttccac tttgcccatc gctttttcag ccgttccttc ggggatttgt
     2041 ttcgatattt gacccagttc gcccggcatt ttcgacatca ggttttccaa accgcccata
     2101 ttgcgcattt gctggatttg ttctttaaag tcgttgaggt cgaagccttt gcctttttgt
     2161 agctttttcg ccattttggc ggcggcttct tcatcaatac ctttttggac gtcttcaatc
     2221 agggtcaata cgtcgcccat gccgaggata cggctggcaa gacggtcggg gtggaaaggt
     2281 tcgaggccgt tgattttttc gccgacaccg ataaatttaa tcggcttgcc ggttacgtgg
     2341 cgcacggaca aagctgcgcc gccgcgcgaa tcgccgtcca tcttggtcaa tacgacaccg
     2401 gtcagcggca gggcttcatt aaatgcctga gcagtgttca ccgcatcctg acccagcatc
     2461 gcatcgatga cgaacaaagt ttccaccggg ttaaccgccg cgtgaagggc tttgatttcg
     2521 ttcatcatct cctcatcgat tgccaaacgg ccggcggtat cgaccatcaa tacatcgtaa
     2581 aaatgttttt tggcgtaatc gacggcggca gttgcaatct caaccggttt ttggttggta
     2641 tcggatggga aaaagtccac gccgacctgt tcggccaaca gacgcagttg ttcaatcgcg
     2701 gcagggcggt aaacgtcggc agatacgacc aaaacctttt tcttctgatc gtttttcagc
     2761 aggcgggcga gtttgccggc ggtcgtcgtc ttgcccgcgc cctgcaaacc tgccatcaac
     2821 acgacggcgg gcggcgcaac cgacaaatcc agcgttttgt tttccctgcc catcagctcg
     2881 gtcagggttt tgttgaccac gccgataaat gcctgatccg gcgtcaggct gcccaccact
     2941 tcctgaccga gggccttttc tttgacgttg ttgacgaact ctttgacgac aggcagggcg
     3001 acatccgcct caagcagggc gaggcggact tcgcgcaagg cttctttaat attgtcttcg
     3061 gtcagtttgg cctgcccgcg gatgtttttg agaacattgc tgaagcggcc ggttaaattg
     3121 tctaacatac tggtccttgg tctgaataag aatagcttgc cccatcaggg gcattctttg
     3181 ttaaaataaa atcaaaataa tttgatgcgg cttgtgtgcc ggacagcata tcggcaaatc
     3241 cgtcaaggct tgcccgaaac ggggatttta caattccaac gttaaaagtt ccaatatttc
     3301 ataagcggcc gcatacagcg caacagtata gattagagaa agtccaccat gccgacagtt
     3361 ttcatctttt tgacggcggt ttacgcagga ttgggtgcat ttgcatggca ctgccaacag
     3421 caggggctcg gccgggatta cccgtggaag acggaattgc cggttttggg cgcggcattg
     3481 accgtccatg gcgcggcact gcttatgccg gtcattcaag acaaaatcat cattatgggc
     3541 tttgggtatt ccggcagcct gattgtttgg atgatgctgt ttatttattt tgccggcagc
     3601 ttcttttatc cgctgcgcgg agtgcagttg ctgctgtatc cttgcgccgc actgatgctg
     3661 ctgtcaggtt tggtttttcc tggaaaattc tcgggatatg aaattaccga ccttcccttt
     3721 atgctgcata tcggaacttc gctgcttgca tacgggctgt tcggcatcgc aacattattg
     3781 tccgttttga ccctgctgct gaatcggagc ctgcaccgca ggaacttctc caagctcgca
     3841 ggattcctgc cgtcgctgct cagtttggaa aaactcatgt tccaggccat gtgggcaggt
     3901 ttcatcctgc tgacctattc cgtcgtcagc ggaacatttt ttgccgaagc cgtattcggc
     3961 aaacccatga cctttaccca taaaaccgta ttcggcatat tgtcatggct gatttacggc
     4021 ggactgctgc tcaagcacag catgaccgca tggcgcggca aaaaagccgc cgtgtggacc
     4081 atcatcggat ttgtcagcct gatgattgcc tatatgggca gcaagttcgt attggaaatc
     4141 attctgaaaa gataagaaga gccaacagat gccgtctgaa gccccgagtt tcagacgg
//
LOCUS       DAXHTR010000154         4153 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.154, whole genome shotgun sequence.
ACCESSION   DAXHTR010000154 DAXHTR010000000
VERSION     DAXHTR010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.154"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(392..826)
                     /locus_tag="ACFNML_001816"
     CDS             complement(392..826)
                     /locus_tag="ACFNML_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HFC6393137.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(829..1491)
                     /gene="exbB"
                     /locus_tag="ACFNML_001817"
     CDS             complement(829..1491)
                     /gene="exbB"
                     /locus_tag="ACFNML_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HFC6393138.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            complement(1557..2399)
                     /locus_tag="ACFNML_001818"
     CDS             complement(1557..2399)
                     /locus_tag="ACFNML_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HFC6393139.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            2654..3292
                     /locus_tag="ACFNML_001819"
     CDS             2654..3292
                     /locus_tag="ACFNML_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HFC6393140.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            complement(3402..4153)
                     /locus_tag="ACFNML_001820"
     CDS             complement(join(3402..3934,3934..4153))
                     /locus_tag="ACFNML_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242698.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element IS1301 family transposase"
                     /protein_id="HFC6393141.1"
                     /translation="MARTAITDNIWEQLQTTMKAHGCHQWKNDRTVMEAILWKLRTGA
                     PWRDIPIELGSWKTAYNRFNRWSKKGLWQKFFFDLRKEIDKEWVFIDGSYVRCHQHAS
                     GARRGFDRAIGQSRGGNTTKIHLCVDSHGNPLDFKVTGGNVHDSQVANDLIEVIQEAQ
                     YFIADKGYDSQEIRDKAIEHGMKAIIPKRKNAKKPNPDFDSYLYKLRHLVENAFARLK
                     QFRSIATRYEKLARNFKSMLYLACSIIHAKLN"
BASE COUNT          933 a         1125 c          948 g         1147 t
ORIGIN      
        1 gggtatagcg gattaacaaa aaccagtacg gcgttgcctc gccttaactc aaagagaacg
       61 attctctaag gtgctgaagc accaagtgaa tcggttccgt actatttgta ctgtctacgg
      121 cttcgttgcc ttgtcctgat ttttgttaat ccactataaa aattagaaat gcacattttc
      181 attattctcg cgcaggcagg actccagact tacccatttc agtaatgttt gaaaataaaa
      241 gaaaaatcag atgtttgtat tcccgcctgc gcagaaatgg agacggtgct ctgtcgtctc
      301 atttttgttt taatcaacta tatatagctg attaaacata agaaatgccg tctgaaagac
      361 tttcagacgg cattcgttca agcgtcgaac tttattgcgc cttggtttcg gttacaaaac
      421 cgattttggt gattcctgcc tgacgggcgg cttctaaagc tttgtttaca taatcgtatt
      481 ccaccgcctt gtctgccgca atcgccacaa tcacgttttc attctgctcc ttggcggctt
      541 tcagacggct ttccacttcc ccgatttcca ctttgcttgc agaatccccg ccgacataat
      601 agccgccgtt cgcatcaatc gtcaggcgca gggggtcttt aggctgtttg tcctgcttgt
      661 ttgtctgctc ggacgcggtc ggcagttcca aagggatgga atgcgtcagc accggcatag
      721 taatcataaa cacaatcagc aacaccagca tcacgtccac caacggcgta acgttgatgt
      781 cggacatcgg agaatcgtcg ccggaattca tcgaaccaaa tgccataatc agctatcctt
      841 ttgattaagc aggcggacgt gcaaatcgtg cgccatcgca tccaaatcct gggtcagtat
      901 ttttgtgccg cgattgagga agttgtatgc caacaccgcc ggaatcgcca cgaacaaacc
      961 cgccgccgtc gccaccagtg cctcgccaat cgggccggca accgccgcaa tactcatctg
     1021 cccgctttgc ccgatattga tcagggcgtg gtaaatcccc caaaccgtgc cgaacagccc
     1081 gataaacggc gcggtcgcgc cgatggaggc aagcgcggtc atcccgtaat caaaccggcg
     1141 cataatctgc gccatactgt tgcggatttg aatgaccaaa tactcgttca acggcaaagc
     1201 ctgcgccagt tcggacgctt cgtttcggcg gtagttgcgg taagactgca atgcctcttg
     1261 cgccagtttg gacaaaggcg catcgacggc gcgcactttt tcgaccgcgt cgttcagcga
     1321 caaagtatcg cgcatatgcc gtttgacggc ggcattccct ttgcgcgccc gatacagctt
     1381 gatgcagcgc aagacaacca aacaccacgt tacgatactc atcaacagca tcaacacaaa
     1441 cacaccgatc aggacgggat cgcccgattc aaatactaat ttcaaattca taatgattcc
     1501 aacactgaaa aaaccaatca aacatccaag ctgccgcaaa ccgctgcggc aaccgcctaa
     1561 ttcaattcaa acttgacggg gactttaaac tccgtccagg cattggcttg aaaatgcccg
     1621 ttttgcgccg ccttgcgtgc cgcattgtcc aaccgagaaa aaccactgct tttcacgatt
     1681 ttaacggact cgacatgacc gcccggagaa accaaaacgc tcaaaacaac cgtaccctgc
     1741 tcgtcattct ccatagaaag cgtgggataa gccgggcgcg gaatgctgcc gttggcgcgt
     1801 aaaggattgc ctttgctgct gccggctcct tccccgtgtt cgcctttgac accgccgcta
     1861 cctttaccgc tgccttctcc gcgccccgtt ccgtctcctt tggtaccggt tcccttatct
     1921 tccccattac cctgctcgct gtctgctttg gcagaagcat tgctgggatg ttcggcaggt
     1981 ttttcagacg gcttctcgac cggtttttcc gccggtttcg ggacaggctt cgcttccggc
     2041 ttaggctctg gtttcggttt ttcttcgggt ttcggctttt cttcaggttt cggctcttcc
     2101 ttaggctgct gaatatccgc atccgccttt ttcgtaacca ccggcttcaa aaccggcttg
     2161 ggcggctcga caggtttggg cggctcaggc tcaggttgcg gctcaggcgc agcaggcgca
     2221 cctgcgcctt cgggtgcacc gtcccctccg ccaaaatcac ccaaatcgac aaattcgata
     2281 acatttcctg actctatcac gggcagcttg tgcgcctgcc agagcaatgc caccattgcc
     2341 aaatgcagca gtgcgacgga aaacacgact gcgggggtta aaattcgttc tttatccata
     2401 attcgggcat aataatagca acaattccta tttgcaacct atttttacaa tttttggtca
     2461 tatgaatgtc tgttccgttc acaggcaaac cgtgtttaaa cgctgtatta cagcaaatca
     2521 tcagataacg ggccggcaga aaaaatgatt ccgtctgatt tcttattcca ataaaatcag
     2581 gttagatgat atattgccgc ttctgtctgt cagccgtttc gggctgcaca ccacatctgt
     2641 tcaaaggaaa accatgtttc aaaattttga tttgggcgtg tttctgcttg ccgtcctgcc
     2701 cgtgctgctc gccattaccg tcagggaggt ggcgcgcggc tatacggcgc gctactgggg
     2761 agacaacact gccgaacaat acggcaggct gacactgaac cccctgcccc atatcgattt
     2821 ggtcggcaca atcatcgtac cgctgcttac tttgatgttc acgcccttcc tgttcggctg
     2881 ggcgcgtccg attcctatcg attcgcgcaa cttccgcaac ccgcgccttg cctggcgttg
     2941 cgttgccgcg tccggcccgc tgtcgaatct agcgatggct gttctgtggg gcgtggtttt
     3001 ggtgctgact ccgtatgtcg gtggggcgta tcagatgccg ttggctcaaa tggcaaacta
     3061 cggtattctg atcaatgcga ttctgttcgc gctcaacatc atccccatcc tgccttggga
     3121 cggcggcatt ttcatcgaca ccttcctgtc ggcgaaatat tcgcaagcgt tccgcaaaat
     3181 cgaaccttat gggacgtgga ttatcctact gctgatgctg accggggttt tgggtgcgtt
     3241 tattgcaccg attgtgcggc tggtgattgc gtttgtgcag atgttcgtct gactggcttt
     3301 cagacggcat aaacgctcca gaaaacgcgg caggacatat tgccctgccg cgttttcctg
     3361 tagtgtaatc ttattttttt catcattatt agggcgtgtc ctcaatttaa cttagcatga
     3421 ataatagagc aagccaagta aagcatcgat ttaaaattac gagctaattt ctcatagcga
     3481 gtagcaatac tacgaaactg ctttaatcgt gcaaatgcat tctcgactaa gtggcgtaat
     3541 ttataaaggt agctatcaaa atcagggtta ggtttcttgg catttttacg ctttggtata
     3601 atagctttca taccgtgttc tatcgcttta tctctgattt cttgcgagtc ataccctttg
     3661 tcagcgataa aatactgggc ttcttgtatg acttctatca agtcgtttgc aacttgactg
     3721 tcgtgcacgt tacccccagt gactttaaaa tcgagcggat ttccatgcga gtccacacat
     3781 aggtgtattt ttgtcgtgtt tccgccacgg ctttgtccaa ttgctctatc gaaaccacgc
     3841 cgagctccac ttgcatgttg atgacaccgt acataacttc cgtcgatgaa tacccattct
     3901 ttgtcaattt cttttcgtag atcaaaaaaa aattctgcca caagcctttt ttagaccatc
     3961 ggttaaagcg gttataagcg gttttccatg accctagctc aataggtatg tctcgccatg
     4021 gtgcacctgt tcttagcttc catagtatgg cttccatcac ggtacggtcg ttcttccatt
     4081 gatgacagcc gtgcgctttc atggttgttt gtaattgttc ccatatgttg tcagttattg
     4141 cggttctcgc cat
//
LOCUS       DAXHTR010000155         4131 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.155, whole genome shotgun sequence.
ACCESSION   DAXHTR010000155 DAXHTR010000000
VERSION     DAXHTR010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.155"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            43..675
                     /locus_tag="ACFNML_001821"
     CDS             43..675
                     /locus_tag="ACFNML_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HFC6393142.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFSCRLARRLSDSGIAVISGGGLGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="ACFNML_001822"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="ACFNML_001822"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFC6393143.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKER
                     FSGVPEVAAASEFIHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKVFIESLEIPAEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2198..4067
                     /locus_tag="ACFNML_001823"
                     /pseudo
     CDS             2198..4067
                     /locus_tag="ACFNML_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
BASE COUNT          945 a         1118 c         1069 g          999 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttctcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggctgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcgggc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttccgaa tacggcctga
      841 tgaaagcgcg cgtcaaagtc gaattaaact ggctcaaagc cctcgccgcc gagccgaaga
      901 ttgtcgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgcg gccgccgtca aagccatcga agccaccacc aatcacgatg
     1021 tcaaagccat tgaatattgg cttaaagaac gcttctccgg cgtgcctgaa gtggcagccg
     1081 ccagcgagtt catccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggac
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtc gcctatcctg atgtagattg ggaaacccac tgccgcaact
     1441 tcgtcgaaat cagcctcggt ctgaccttca acccctacac catccaaatc gaaccgcacg
     1501 actatatggc ggaattcttc caaaccctca gccgcatcaa cacgattctg atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcgggcg
     1621 aagtcggctc ttccacgatg ccgcacaaag tcaaccccat cgactttgaa aactccgaag
     1681 gcaacctcgg tatggcaaac gccgtattgg gctttttgtc agaaaaactg ccgatttccc
     1741 gttggcagcg cgacctgacc gacagcaccg tcctgcgcaa tatgggcgtg ggcgtgggct
     1801 atgccgtgtt gggtttcgcc gcccacctgc gcggtctgaa caagctcgaa cccaaccctg
     1861 ccgcgcttgc cgctgatttg gatgccactt gggaactgct cgccgagccg attcaaaccg
     1921 tgatgcgccg ttacggcgtg gccaatcctt acgagaagct gaaagacctg acgcgcggta
     1981 aaggcggcat tacgcccgaa gtgctgaaag tcttcatcga atcgttggaa attccggcag
     2041 aagccaaagc caaattgctt gagctgaccc ccgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacgggggc gatatttatg caagctgtcc gatacagacc
     2221 ggaaattgac ggattgcggg ccgtcgccgt gctatccgtc atgattttcc acctgaataa
     2281 ccgctggctg cccggaggat tcctgggggt ggacattttc tttgtcatct caggattcct
     2341 cattaccggc atcattcttt ctgaaataca gaacggttct ttttctttcc gggattttta
     2401 tacccgcagg attaagcgga tttatcctgc ttttattgcg gccgtgtcgc tggcttcggt
     2461 gattgcctct caaatcttcc tttacgaaga tttcaaccaa atgcggaaaa ccgtggagct
     2521 ttctgcggtt ttcttgtcca atatttatct ggggtttcag caggggtatt tcgatttgag
     2581 tgccgacgag aaccccgtac tgcatatctg gtctttggca gtagaggaac agtattacct
     2641 cctgtatcct cttttgctga tattttgctg caaaaaaaca aaatcgctac gggtgctgcg
     2701 taacatcagc atcatcctat ttctgatttt gactgccaca tcgtttttgc caagcgggtt
     2761 ttataccgat attctcaacc aacccaatac ttattacctt tcgacactga ggtttcccga
     2821 gctgttggca ggttcgctgc tggcggttta cgggcaaacg caaaacggca gacggcaaac
     2881 agcaaatgga aaacggcagt tgctttcatc actctgcttc ggcgcattgc ttgcctgcct
     2941 gttcgtgatt gacaaacaca atccgtttat cccgggaatg accctgctcc ttccctgcct
     3001 gctgacggca ctgcttatcc ggagtatgca atacgggaca cttccgaccc gcatcctgtc
     3061 ggcaagcccc atcgtatttg tcggcaaaat ctcttattcc ctatacctgt accattggat
     3121 ttttattgct ttcgcccatt acattacagg cgacaaacag ctcggactgc ctgccgtatc
     3181 ggcggttgcc gcgttgacgg ccggattttc cctgttgagt tattatttga ttgaacagcc
     3241 gcttagaaaa cggaagatga ccttcaaaaa ggcatttttc tgcctctatc tcgccccgtc
     3301 cctgatactt gtcggttaca acctgtacgc aagggggata ttgaaacagg aacacctccg
     3361 cccgttgccc ggcgcgcccc ttgctgcgga aaatcatttt ccggaaaccg tcctgaccct
     3421 cggcgactcg cacgccggac acctgcgggg gtttctggat tatgtcggca gccgggaagg
     3481 gtggaaagcc aaaatcctgt ccctcgattc ggagtgtttg gtttgggtag atgagaagct
     3541 ggcagacaac ccgttatgtc gaaaataccg ggatgaagtt gaaaaagccg aagccgtttt
     3601 cattgcccaa ttctatgatt tgaggatggg cggccagccc gtgccgagat ttgaagcgca
     3661 atccttccta atacccgggt tccaagcccg attcagggaa accgtcaaaa ggatagccgc
     3721 cgtcaaaccc gtctatgttt ttgcaaacaa cacatcaatc agccgttcgc ccctgaggga
     3781 ggaaaaattg aaaagatttg ccgcaaacca atatctccgc cccattcagg ctatgggcga
     3841 catcggcaag agcaatcagg cggtttttga tttggttaaa gatatcccca atgtgcattg
     3901 ggtggacgca caaaaatacc tgcccaaaaa cacggtcgaa atacacggac gctatcttta
     3961 cggcgaccaa gaccacctga cctatttcgg ttcttaatta tatggggcgg gaattccaca
     4021 aacacgaacg cctgcttaaa tcttcccacg gcggcgcatt gcagtagcct gccttcttgt
     4081 cggatattgc ctttggcagc ctatgccgct gtttgccgtt cggggcggcg g
//
LOCUS       DAXHTR010000156         4121 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.156, whole genome shotgun sequence.
ACCESSION   DAXHTR010000156 DAXHTR010000000
VERSION     DAXHTR010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.156"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..144
                     /locus_tag="ACFNML_001824"
     CDS             <1..144
                     /locus_tag="ACFNML_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HFC6393144.1"
                     /translation="NVGIGSGALLGHWVTQYVGISRIGIAGMLAAAAGLWLCLNLNRH
                     IRT"
     gene            272..1708
                     /locus_tag="ACFNML_001825"
     CDS             272..1708
                     /locus_tag="ACFNML_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HFC6393145.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGKGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFSWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRDALCRLKYLAPLSWFVTFHFVCLSFVVFNTANPD
                     DAGAVFSALFANASGWNAPQQADMLLLASFASVMLLYPYLQRAFDGVVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            1719..2702
                     /gene="patB"
                     /locus_tag="ACFNML_001826"
     CDS             1719..2702
                     /gene="patB"
                     /locus_tag="ACFNML_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan O-acetyltransferase PatB"
                     /protein_id="HFC6393146.1"
                     /translation="MKNFLSLFASILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGVALQENAYALSDGIKAFLSGETPPTAQDGGSADMPSEAAASEAVPQTGET
                     EWKQDTEAAAVRSGDKVFFAGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLKKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     YTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            2699..3892
                     /locus_tag="ACFNML_001827"
     CDS             2699..3892
                     /locus_tag="ACFNML_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HFC6393147.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKIRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRHNGNWQSLTSRNNSGDFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQIQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAIRQ"
BASE COUNT          879 a         1343 c         1053 g          846 t
ORIGIN      
        1 aacgtcggta tcggcagcgg cgcactgttg ggacattggg taacgcaata tgtcggtatt
       61 tcccgtatcg gcatcgccgg tatgcttgcg gcggcggccg ggctgtggct gtgcctgaac
      121 ttgaaccgcc atatccgaac ctgacccgcg atgccgtctg aagcctctcc cgcccttcag
      181 acggcatttt gattgaagaa atatccaccc ctgcttaaaa taacgggctt tgccgtgttt
      241 taacgcctat tttttgttcc aaggatggtt tatgccgctg ctgtctgtcg agttcgcact
      301 gttctttctc gccttcctgc cgatttactg gggcttggcg aaatacccgt ccgtccaaaa
      361 cctgctgctt ttggctgccg gtatgggctg gctctaccat atcggccccg tatttgcggc
      421 aatcgtcgtc ctttattcct cctgcgtgta ccttttgggc gaactgctcc gttccgatcg
      481 cgaaaatacg cgccgctttt ggctggggtg cggcattgcc gtctcgctga ccgtcttggg
      541 ctttttcaaa tatttcgact ttttccgccc gctgattgcc caatatgccg gaaaaggcgg
      601 cgcaatcgac atcctgatgc cgctggggct ttcgtattac accttccagt cgctcgccta
      661 tctggtttac tgcttccgcg ccccgcacgc tgcgcgtttc agctggcacg agctgctact
      721 acacctgagt ttctttccca ccgttacctc tggcccgatt atccgcgccg ccgcattcaa
      781 aagcgcggac ggcgagcagg cgggcgcatt ggcgcaaatc cgtacccgcc aaccgcgctc
      841 acccgtccgc cccgcactcg ccgtttccct gattctgctg ggtattgcca aaaaatggtg
      901 gctggcgggg atgctggcgg aaaactgggt gtcgcccgta tttgaaaatc ccgcccaatt
      961 cgacggctgg ggcgtattgg gcggcgtgta cggctatacc ttccaactct ttttagactt
     1021 ttccggatat tccgatttgg ttatcggcat ggcgatgctg ctgggtttcc ggctgcccaa
     1081 aaacttctcc gcaccgcttc gtgctttaaa catccgcgca ttttgggaca aatggcacat
     1141 cagcctttcc acctggatac gcgactacat ctacatcccc ttgggcggca gcaaaaaagg
     1201 ctttttacgg acacagctca acctgatggc ggcaatggta ctctccggca tatggcacgg
     1261 ctacggctgg aacttcctca tttggggcgc gctgcacggc acggcgctgg tgctgctcaa
     1321 cacgggcgac cgttatttcg gacgcgacgc gctatgccgt ctgaaatacc ttgcgccgct
     1381 ctcgtggttt gttaccttcc attttgtctg ccttagcttt gtcgtcttca ataccgcaaa
     1441 ccccgacgat gccggcgcgg ttttcagcgc gctctttgcc aatgccagcg gctggaatgc
     1501 gccgcaacag gcagacatgc tgctgcttgc ctcgtttgca tccgtgatgc tgctctaccc
     1561 ttacctgcaa cgcgctttcg acggcgtggt caaaggtttg gaaaaaatcc cgatgtggct
     1621 gtggtttatc cccgtttccg ccgtcctgct gctgattatc gtcctcgccc cctcggggat
     1681 acccggcttt atttatgcca atttttaagg gtttggacat gaaaaacttt ctttcccttt
     1741 tcgcctccat attgatgtct gccctgattg ccgtgtggtt cagccaaaac cccatcaacg
     1801 cctactggca gcagacctac caccgcaaca gcccgctcga accgctcgcc gcctacggat
     1861 ggtggcggag cggtgtggcg ttgcaagaaa acgcctacgc cctttcagac ggcatcaaag
     1921 ccttcctgtc cggcgaaacg ccgccgacgg ctcaagacgg cggttcggca gatatgccgt
     1981 ctgaagccgc cgcatcagaa gccgtccctc aaaccggtga aacagaatgg aaacaagaca
     2041 ccgaagccgc cgccgtccgc agcggcgaca aagtcttttt tgccggcgac tcgctgatgc
     2101 agggcgttgc acccttcgtg caaaaaagcc tgaaacagca atacggcatc gaatccgtca
     2161 acctcagcaa acaaagcacg gggctgtcct acccctcatt cttcgactgg ccgaaaacga
     2221 ttgaagaaac cctgaaaaaa catcccgaaa tcagcgtgct cgccgtcttc ctcggcccga
     2281 acgacccgtg ggatttcccc gtcggcaaac gatacctcaa attcgcttcc gacgaatggg
     2341 cgcaagaata cctgaaacgc gtcgaccgca tccttgaagc cgcacacacg caccgcgtcc
     2401 aagtcgtctg gctcggcatc ccctatatga aaaaagccaa gctcgacggg cagatgcgct
     2461 acctcgacaa actgctttcg gaacacctgg aaggcaaaat catcctgatt cccaccgcgc
     2521 acaccctgag cggcggggaa gaccgctaca ccgactccgt caacgtcaac ggcaaacccg
     2581 tccgctaccg cagcaaggac ggcatacact ttaccgccga aggacaaaaa ctgctggcgg
     2641 caaaaataat ggaaaaaatc gtttttgaac caagtacgca accatcaagt acacagccat
     2701 gaaccccaaa cacctcatcg cattttccgc cctgttcgcc gccacgcagg cagaagccct
     2761 gcccgtcgcc tccgtcagcc tcgacaccgt taccgtttcc ccgtccgccc cctacaccga
     2821 tacaaacggg ctcttgaccg actacggcaa cgccgccgcc tcgccttgga tgaaaaaaat
     2881 ccgatccgtc gcacaaggca gcggcgagac cttccgcatc ctgcaaatcg gcgactcgca
     2941 taccgccggc gacttcttta ccgacagcct gcgcaaacgc ctgcaaaaaa cttggggcga
     3001 cggcggcata ggctgggttt accccgccaa cgtcaaaggg cagcgcatgg cggcagtccg
     3061 gcacaacggc aactggcaaa gcctcaccag caggaacaat agcggagact tcccgctcgg
     3121 cggcatcctc gcccacaccg gcagcggcgg cagcatgacc ctgaccgcat cggacggcaa
     3181 gaccggcaaa cagcgcattt ccctgtttgc caaacccctg ctcgccgaac aaaccctgac
     3241 cgtcaacggc aacaccgtct ccgccaacgg cggcggctgg caggtactgg atacccgcgc
     3301 ggcactgccc ttgaccatac agaccgaaat gccgtgggac atcggcttca tcaacatcga
     3361 aaatcccgcc ggcggcatta ccgtttccgc catgggcatc aacggcgcac aattgaccca
     3421 ttggtcgaaa tggcgtgccg accgtatgaa cgaccttgcc caaaccggcg ccgatttggt
     3481 tatcctttcc tacggcacca acgaagcctt cggcgacaac atcgacattg ccgacaccga
     3541 acaaaaatgg ctggataccg tccgccaaat ccgcgacagc ctgcctgccg ccggcatcct
     3601 catcatcggc gcacccgaat ccctgaaaaa cacgctcggc gtatgcggca cacgccccgt
     3661 ccgcctgacc gaagtccaac agatacagcg gcgcgtcgcc cggcagggac aaaccatgtt
     3721 ctggtcttgg caaaacgcca tgggcggcat atgcagcatg aaaaactggc tcaaccaagg
     3781 atgggccgcc aaagacggcg tacacttctc cgccaaaggc taccggcgcg cggcggaaat
     3841 gctcgccgac agcctcgaag aactcgtccg cgccgctgca atcaggcaat aatcggacag
     3901 gaggcggacg gtatttccgc aacaggggga tgccgtctga aacgcatacc ttcatattgc
     3961 ttcagacggc atagccaccc cgcgcacggt ttgccggacg caaccggcat tcgcctcaag
     4021 catcggaagg acgggggcga acctccggca tacggcgcaa aggcggcgtt tgatatgccg
     4081 tctgaaggca aagatgataa actgccgcct tccgttttca g
//
LOCUS       DAXHTR010000157         4005 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.157, whole genome shotgun sequence.
ACCESSION   DAXHTR010000157 DAXHTR010000000
VERSION     DAXHTR010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4005
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.157"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(69..1319)
                     /gene="glyA"
                     /locus_tag="ACFNML_001828"
     CDS             complement(69..1319)
                     /gene="glyA"
                     /locus_tag="ACFNML_001828"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFC6393148.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     EAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLIAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSAIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADARVLANLVADVLSNPE
                     DEANLENVRKQITALCDKYTVYGA"
     gene            complement(1474..1737)
                     /locus_tag="ACFNML_001829"
     CDS             complement(1474..1737)
                     /locus_tag="ACFNML_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393149.1"
                     /translation="MSNQTKIQAIKQASEQIFAICETPNTALQAIHLILRHGGAGELS
                     WQVVYQRVMADKDVVGAGYLIDFAQTAENLPFDVLPLGSLVLT"
     gene            complement(1800..3575)
                     /gene="ggt"
                     /locus_tag="ACFNML_001830"
     CDS             complement(1800..3575)
                     /gene="ggt"
                     /locus_tag="ACFNML_001830"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFC6393150.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLIEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3743..4005)
                     /locus_tag="ACFNML_001831"
                     /pseudo
     CDS             complement(3743..4005)
                     /locus_tag="ACFNML_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
BASE COUNT          997 a          954 c          943 g         1111 t
ORIGIN      
        1 tctccgcctg aaacattgtg agatttcagg cggcattgga ttgcttggcg gaatattttt
       61 aaaaaggctt acgcgccgta aacggtgtat ttgtcgcaga gggcggtaat ctgtttgcgg
      121 acgttttcca gattcgcttc gtcttcgggg ttagacaata cgtcggcaac caagttcgcc
      181 aatacgcgcg cgtcggcttc gttaaaaccg cgtgtggtca tggcagcgga gccgatgcgg
      241 atgccggagg taacgaaggg tttttccgga tcgttcggga tggcgttttt gttgacggtg
      301 atgtgcgctt tgcccaaagc ggcttcggcg gctttaccgg tgattttcat cggttgcagg
      361 tcgacgagga aaacgtggct ttcggtgcgg ccggaaacga tgcgcaaacc gcgtttaacc
      421 aactcttctg ccatggcggc agcattgatt ttcacttgtt ttgcgtattg tttgaactcg
      481 ggttgcaacg cttctttaaa cgctacggct ttggcggcga tgacgtgcat cagtggaccg
      541 ccttgtaggc ttgggaagat ggcggagttt aacgcttttt cgtgggtatt gtcgcggcac
      601 aaaatcacgc cgccgcgagg gccgcgtagg gttttgtggg tggtggtggt cacgaagtcg
      661 cagaatggca cggggttggg atattcgcca ccggcaatca gtccggcata gtgcgccatg
      721 tcgacaaaga ggtatgcgcc gactttatcg gcgatctcgc ggaattttgc ccagtcgatt
      781 tgcaacgcgt aggcagacgc accggccaca atcattttag gtttgtgttc gagagccaag
      841 cgttccactt cggcataatc gagcacttcg ttttcatcca aaccataagt aacggcattg
      901 tagagtttgc cggaaatatt aacgctcgcg ccgtgggtca ggtggccgcc gtgcgccaaa
      961 gacataccca aaatggtgtc gcccggtttt aaaacggaag cgtacacggc ttggttggct
     1021 tgcgagccgg agtgcggttg aacgttggcg tattgcgcgc cgaacagttt tttgacgcgg
     1081 tcgattgcca attgttcgac aatatcgacg tattcgcagc cgccgtagta gcgtttgccg
     1141 gggtagcctt cggcgtattt gttggtcagc tgggaacctt gcgcttccat tacggcgcag
     1201 ctgacgtagt tttcagaggc aatcagctcg acgtggtctt gctggcgttg gtcttcttgg
     1261 gcaatggctg ctgccaaatc ggggtcatat tgtgcgaggg taacgctttt tgaaaacatg
     1321 ttctcggctc ctttgtgtaa tcagggtatc atgagtgttt tttgtataaa aaaatacttc
     1381 aaaacctaag gcagatagtc cataatgcgt aaattttctt tggcattatc aggtaattta
     1441 tttaacatgc tggtttttag cgtctcatta cctttatgtt agtacaaggc tacccaaagg
     1501 caatacatca aatggtaaat tttcggcagt ttgtgcaaaa tcaatcaaat aaccagcacc
     1561 aaccacatct ttatcagcca taacccgctg atacaccact tgccaagaca actctcctgc
     1621 cccaccatgt cttaaaatca gatgaatggc ttgtaatgct gtgtttggcg tctcacaaat
     1681 tgcgaagatc tgctcagaag cttgcttgat ggcttgtatt tttgtttgat tactcacaat
     1741 ttccccctat tttaatatta acttaatgcg gtcaaattca caaaatacaa gcttcacctc
     1801 taatcaccca tcactcgacc ttctcggcgt ggatcggcac caccaaccag cctgcttggc
     1861 tcgataataa tggcttgaac acctgaattt agctcacgca cgtcagtctt atagcccaaa
     1921 tcatttaatg cttgttgcca ctggagggcg gttgtacccg tttctagttc atagttacca
     1981 aagcgattta ataaattggg tgcactgatg gcatcttgga tatccatatt ccagtcacta
     2041 tgtgccacaa tcgtcttagc gacatagcca atgatacggc taccacctgg ggagccgatt
     2101 gccatataag gcttgcctgc tttaaatacg atggttggtg ccattgagga gcgtggtctc
     2161 ttgccgggct cgacacgatt ggcgacctgt ttgccctgct ttattggctc aaaactaaag
     2221 tctgtcagct cattattcag caggtagcca tttgccatca aagttgagcc aaacgcattt
     2281 tcaatggaag tcgtcattga tagcacattg cccgccttat ccacaattga tatatgactg
     2341 gtagaaggta actcaatcgc ttgtgaggac acccactcat gaataaaatc gcctgcagat
     2401 acgctaggca atgccttatc cgactgctca agcagctggc tgcgatgttt taggtagtct
     2461 ttagaaatca actggcgaat gggtactggt acaaaatcag ggtcgcccaa atatacatca
     2521 cgatccgcaa acgcaagcct agaagcgtcg cccaagagac gtaaaccttc agcatcatac
     2581 cccacctgat tgggtgaaaa ttcatttaaa atccccaaaa tttgacccac agcaatccca
     2641 cctgagcttg gtgcacccat accgcatact tcataaatac gataagtcac acaaacaggc
     2701 gggcgttcca ccacttgata atcagataaa tcttgtaagg ataattgacc ggggttatcc
     2761 ttagcatttt ggacaactga aacgatattt tgggcatatt taccagtatg cagagctttt
     2821 gcaccttgag ctgctaacgc ctgaacactg tcagcaaatt ctaaattttt cagcaagctg
     2881 cctgcttgta gcggcacacc attcggcaaa aaataagcgg ctgtttttgg atagcgtgcc
     2941 aaatgctgct gattttgctc aaccgagatg gcaagccttg gcgacacctc aaagccttgt
     3001 tttgccaagc ggatcggtgt atcaaataat tttccccaag gcaatacacc gtatcgctga
     3061 tgtattgtct ccatcagttt agggatagca ggcgtaccca ccgagcgacc accgaccacc
     3121 gcttccataa atttcaatgg ttgaccatct ttatccaaaa ataattccgg cgtcgcacgc
     3181 atcggtgccg tctcacgccc atcaaatgtg gtcaatgttt tggcggtatt atcccaatac
     3241 aacacaaatg caccaccgcc caagcctgac gactgtggct ctaccaagct tagtgtcgtc
     3301 tgcaccgcca ccatcgcatc tgcagcgcta ccgccttgct ttaagatatc atagccagct
     3361 tgtgttgcta atggattggc tgacgctacc ataaaatcac ttgcaatcac ctgcttttgt
     3421 tcgatcagtc ccgttgcatg ttcaggcgtg tgagcgtctg cacctgtgat gacagcagaa
     3481 tgagtattaa ccttaccttg attggcatgg atgacttgac atccagagat tgtcatagac
     3541 attatcaatg cagtcaataa atatgtttta gccacaagca ctccttcgcc tgagtttgat
     3601 tgatgattca tacaaggcat gctgattatt gtatttaata tggctaaata attcaatcca
     3661 aactatcaat cttgaccatc aaaaaaagac cgctaatgtc atcagcagtc ttttttgata
     3721 tttattttaa gatattaagt aatcagacct ttgggctatg ctcttcaatg agtggtttta
     3781 gctcacctga ttggtacatt tgtaggataa tatcactacc accgattaac tcaccattaa
     3841 cccaaagctg tggaaaggtt ggccgattgg cgatgagtgg tagagtactg cgaatttctg
     3901 ggttttctag gatattaaca aaagcaaagg gtctgccaat tgggtcagca cctctactgc
     3961 acgcgctgaa aatccacatt ggggaaactg gggcgtgcct ttcat
//
LOCUS       DAXHTR010000158         3985 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.158, whole genome shotgun sequence.
ACCESSION   DAXHTR010000158 DAXHTR010000000
VERSION     DAXHTR010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3985
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.158"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            156..3269
                     /locus_tag="ACFNML_001832"
     CDS             156..3269
                     /locus_tag="ACFNML_001832"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFC6393151.1"
                     /translation="MAVIIFVNGIRAVNGLVKSSINTANAFAEEGLDVHLINFVGNIT
                     GAEHLSPPFHLHPNVKTSSIIDLFNDIPENVSCRNIPFYSIHQQFFKAEYSAHYKHVL
                     MKIESLLSEEDSIIFTHPLQLEMYRLANNNIKSKAKLIVQIHGNYMEEIHNYEILARN
                     IDYVDYLQTVSDEMLEEMHSHFKIKKDKLVFIPNITYPISLEKKEADFFIKDNEDIDN
                     AQKFKRISIVGSIQPRKNQLDAIKIINKIKNENYILQIYGKSINKDYFELIKKYIKDN
                     KLQNRILFKGESSEQEIYENTDILIMTSQSEGFGYIFLEGMVYDIPILAYNFKYGAND
                     FSNYNENASVFKTGDISGMAKKIIELLNNPEKYKELVQYNHNRFLKEYAKDVVMAKYF
                     TILPRSFNNVSLSSAFSRKELDEFQNITFSIEDSNDLAHIWNFELTNPAQNMNFFALV
                     GKRKFPMDAHIQGTQCTIKIAHKKTGNLLSLLLKKRNQLNLSRGYTLIAEDNSYEKYI
                     GAISNKGNFEIIANKKNSLVTINKSTLELHEIPHELHQNKLLIALPNMQTPLKITDDN
                     LIPIQASIKLEKIGNTYYPCFLPSGIFNNICLDYGEESKIINFSKYSYKYIYDSIRHI
                     EQHTDISDIIVCNVYSWELIRASVIESLMEFTGKWEKHFQTSPKIDYRFDHEGKRSMD
                     DVFSEETFIMEFPRKNGIDKKTAAFQNIPNSIVMEYPQTNGYSMRSHSLKSNVVAAKH
                     FLEKLNKIKVDIKFKKHDLANIKKMNRIIYEHLGININIEAFLKPRLEKFKREEKYFH
                     DFFKRNNFKEVIFPSTYWNPGIICAAHKQGIKVSDIQYAAITPYHPAYFKSPKSHYVA
                     DKLFLWSEYWNHELLPNPTREIGSGAAYWYALDDVRFSEKLNYDYIFLSQSRISSRLL
                     SFAIEFALKNPQLQLLFSKHPDENIDLKNRIIPDNLIISTESSIQGINESRVAVGVYS
                     TSLFEALACGKQTFVVKYPGYEIMSNEIDSGLFFAVETPEEMLEKTSPNWVAVADIEN
                     QFFGQEK"
     gene            3350..3985
                     /locus_tag="ACFNML_001833"
     CDS             3350..3985
                     /locus_tag="ACFNML_001833"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFC6393152.1"
                     /translation="MYSEQGINNTINISTTSLTNATQLTVIGNNNSVYIGNNCKIVSS
                     NIRLKGNNITLFIADDVEIMGLVCSLHSDCSLQIQAKTTMGNGEITIAEKGKISIGKD
                     CMLAHGYEIRNTDMHPIYSLENGERINHGKDVIIGNHVWLGRNVTILKGVCIPNNVVV
                     GSHTVLYKSFKEPNCVIAGSPAKIVKENIVWGRKMYHSTMYDDPTLNEFYK"
BASE COUNT         1450 a          607 c          618 g         1310 t
ORIGIN      
        1 ttaataataa aatagattaa gctattctta aattcagaat attgcttatc tatattaaaa
       61 atttctaatt tttaaggttc tgattgaaat cagaacctta tttcaactat tactttttac
      121 tcataatcga attatatact ttaggacttt ataatatggc tgttattata tttgttaacg
      181 gaattcgggc tgtaaatggc cttgttaaat catctatcaa tactgcaaac gcttttgctg
      241 aagaaggact ggatgttcat ttaattaatt ttgttggcaa tattactgga gcagagcatt
      301 tatccccccc attccactta catcccaatg tcaaaacctc cagcatcata gatttattta
      361 atgacattcc agaaaatgtt agctgccgaa atattccttt ttattctatc catcaacaat
      421 tcttcaaagc cgaatacagt gcccactata agcatgtttt gatgaaaatt gaatctttat
      481 tatctgaaga agatagcatt atcttcactc atcctcttca actggaaatg tatcgtttag
      541 cgaataataa tattaagtca aaagccaagc taattgtaca aattcatggt aactatatgg
      601 aagaaatcca taactatgaa attttggcac gaaatatcga ttatgttgat tatcttcaaa
      661 cggtatctga tgaaatgctg gaagaaatgc attcccattt caaaatcaaa aaagacaaat
      721 tagtttttat tccaaacatc acttatccca tttcattaga aaaaaaagaa gctgatttct
      781 ttattaagga taatgaagac attgataatg ctcagaaatt taaacgtatc tctattgttg
      841 gcagtattca gccaagaaaa aaccaattgg atgccattaa aatcatcaat aaaattaaaa
      901 atgaaaatta cattttacag atatatggca aatctattaa taaagattac tttgaattaa
      961 ttaaaaaata tattaaagac aataagttac aaaaccgtat cttattcaaa ggtgaatctt
     1021 ccgagcagga aatttatgag aatacagata tcctaatcat gacatctcaa agcgaaggct
     1081 ttggttatat atttctagag ggtatggtgt acgatatccc tatccttgcc tataatttta
     1141 aatatggagc gaatgatttt agcaattata atgaaaacgc ttcagttttt aaaactggtg
     1201 atatttctgg aatggcaaaa aaaataattg agctattaaa taacccagaa aaatataaag
     1261 aattagttca atataatcac aatcgcttct taaaagaata tgcaaaagat gtggttatgg
     1321 ctaaatattt cactattctt ccgcgcagct ttaataacgt atcattatcg tctgctttca
     1381 gccgaaaaga attggacgaa ttccaaaata ttactttttc tattgaagat tctaatgatt
     1441 tagctcatat ttggaatttc gagctaacca atcctgcaca aaatatgaat ttttttgctt
     1501 tagttggcaa gcgaaaattt ccaatggatg ctcatatcca aggaacacag tgtacgatta
     1561 agatagctca taaaaagaca gggaatttat tgtcgctttt actaaaaaaa cgaaatcagt
     1621 tgaatttatc aaggggatat accttaattg cagaagataa tagctatgaa aaatatattg
     1681 gagcaatatc taataaaggt aactttgaaa ttattgcaaa taaaaagaac tcattagtta
     1741 ctataaacaa aagtacctta gagttgcatg agattcccca tgaactacat cagaataaat
     1801 tactgattgc tttacccaac atgcaaacgc ctctaaaaat tactgatgat aatttaatac
     1861 ctatccaagc ctccataaaa ttagaaaaga ttggaaatac ttattaccca tgtttcttgc
     1921 catctggcat atttaataat atctgcttag attacggtga agaatccaaa attattaatt
     1981 ttagtaaata ttcttataaa tatatctatg actcaattcg tcatattgag caacatacag
     2041 atatatcgga tattatcgtt tgcaatgttt attcttggga acttattcgt gcctcagtta
     2101 ttgagagcct tatggaattt accggaaaat gggaaaaaca ctttcagact tctcctaaaa
     2161 ttgattatcg atttgatcat gaaggtaagc gttcgatgga tgatgtcttt tcagaagaaa
     2221 catttattat ggaatttccg cgtaaaaatg gtatagataa gaaaacagca gccttccaaa
     2281 atataccaaa cagtattgta atggagtatc cgcagaccaa tggttacagt atgcgcagtc
     2341 attcactgaa aagtaatgta gttgcggcaa aacattttct tgaaaaatta aataaaatta
     2401 aggtagatat taaatttaaa aagcatgacc ttgcaaacat caaaaaaatg aatcgaatta
     2461 tttatgagca tttaggcatt aacataaata tcgaagcatt tctaaaacca cgattagaaa
     2521 aatttaagcg tgaagaaaaa tattttcatg atttcttcaa aagaaataat tttaaagagg
     2581 taatttttcc aagcacttat tggaatccag gtattatttg tgctgcacat aaacaaggta
     2641 ttaaggtatc tgatattcaa tatgctgcca ttactcctta tcatcctgcg tattttaaat
     2701 caccaaaatc acattacgtt gctgataaat tgttcttatg gtctgaatat tggaatcatg
     2761 agcttttacc aaatccaaca cgagagattg gttctggtgc cgcatattgg tatgcattag
     2821 atgatgtgag attttcagaa aaactgaatt atgactatat ctttctatct caaagtagga
     2881 tttcttcgcg cttgcttagt tttgcaattg agtttgcatt aaaaaatcct caactacagc
     2941 ttttattttc taagcatcca gatgaaaata tagatttaaa gaacagaatt attcctgata
     3001 atcttataat ctccacggaa tcttctatac aaggcatcaa tgaatctcgc gttgctgtag
     3061 gtgtttattc aactagctta tttgaggcat tagcatgcgg caaacaaact tttgttgtta
     3121 aatatccggg atatgaaatt atgtcaaatg aaatagattc agggttattc tttgcagtag
     3181 aaacacctga agaaatgctt gagaaaacaa gcccgaattg ggtggctgtg gcagatattg
     3241 aaaaccagtt ttttggccaa gaaaaataat ttttaagtta atctttaaaa gtgcaatatt
     3301 tttcttattt taaatattgc actttattct tttaatcata ggaactcaca tgtattctga
     3361 acagggaatt aataatacta ttaacatatc tactacatct ttaaccaatg ccacgcaact
     3421 aactgtgatc gggaataata actctgtata tataggtaat aattgtaaaa tcgtcagctc
     3481 caatattaga ttaaaaggaa ataatataac cttatttata gctgatgatg tagaaatcat
     3541 gggactagta tgttcccttc attcggactg ctctttacaa attcaagcca agaccactat
     3601 gggcaatgga gaaattacca ttgctgaaaa aggtaagatt agtattggca aagattgtat
     3661 gctggcacat ggatatgaaa ttagaaatac tgatatgcat ccaatttatt ctttagaaaa
     3721 tggcgaacga ataaaccatg gtaaagacgt tattatcggt aatcacgtgt ggcttggccg
     3781 caatgttact atattaaaag gtgtatgtat tccaaataat gtagttgtag gttctcatac
     3841 tgtgctatat aaaagtttta aagaacctaa ttgtgtaata gcaggttcac ctgcaaaaat
     3901 agtaaaagaa aatattgtat ggggaagaaa aatgtatcat tcaactatgt atgatgaccc
     3961 tactttaaat gaattttata aataa
//
LOCUS       DAXHTR010000159         3952 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.159, whole genome shotgun sequence.
ACCESSION   DAXHTR010000159 DAXHTR010000000
VERSION     DAXHTR010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.159"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(99..1460)
                     /gene="accC"
                     /locus_tag="ACFNML_001834"
     CDS             complement(99..1460)
                     /gene="accC"
                     /locus_tag="ACFNML_001834"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFC6393153.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(1574..2026)
                     /gene="accB"
                     /locus_tag="ACFNML_001835"
     CDS             complement(1574..2026)
                     /gene="accB"
                     /locus_tag="ACFNML_001835"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFC6393154.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAPVYAA
                     PVPAAAPAVTPAAAPVAASAPAAPAARDLSDAQKSPMVGTFYRAPGPNAAAFVEVGKQ
                     VKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(2008..2265)
                     /locus_tag="ACFNML_001836"
     CDS             complement(2008..2265)
                     /locus_tag="ACFNML_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HFC6393155.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            2382..3422
                     /gene="queA"
                     /locus_tag="ACFNML_001837"
     CDS             2382..3422
                     /gene="queA"
                     /locus_tag="ACFNML_001837"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFC6393156.1"
                     /translation="MDISDFDFTLPEKLIAQHPPEVRGSSRLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDNHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGQTVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLHRLKDKGAVTAEVTLHVGAGTFQPVRVEKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGRNEGVVR"
BASE COUNT          839 a          963 c         1187 g          963 t
ORIGIN      
        1 ttaatccact atatcgaaat cggtttcaga cggcgcgggg caagggaaat gccgtctgaa
       61 cgcggacggg cggcttcaga cggcatatcg gcggcggttt acttgtcctg tttggctttg
      121 cgatcttcca gccagtgttc caaatagtgg atgctgacgc cgcctttttg gaaacccgca
      181 tcggcgaaca ggtcgcggtg aagcggcgta ttggttttga tgccggttac cgccagctcg
      241 gcgagtgcga cgcgcatttt cgccattgcc tgttcacgcg ttttgccgtg tacgcagatt
      301 ttgccgatca ggctgtcgta gtacggtggg atgcggtagc cttggtaaat gtggctgtcc
      361 acgcggatac cgaagccgcc gggcaggtgg cagctttcaa tcgggcccgg gctggggatg
      421 aagttgtacg ggtcttcggc attgatgcgg cactcaaacg cgtggccttc gacttgaata
      481 tccttttgtt tgtattgcaa aggcaggccg gcggcgatgc ggagttgctc ctgcacgatg
      541 tccacgccgg taatcagctc ggtaaccgga tgctcaacct gaacgcgcgt attcatctcg
      601 ataaagaaaa attcgccatc ttcgtacagg aattcaaacg tacctgcgcc acgatagccg
      661 atgcgtttgc aggcgtcggc acaggcgttg ccgattttgg cgcgttcttt ttcagtgatg
      721 aacggagccg gtgcttcctc gatgactttt tggtggcggc gttgcagcga acagtcgcgc
      781 tcggcaaggt agatggcgtt gccgtgttcg tcggcaatca cttggatttc gacgtggcgc
      841 ggacgttgca gatagcgttc catgtaaacc atcgggttgc cgaatgccgc gcctgcttct
      901 gccttggtca tttcgacaga ttggaggagg tcttcttttt tctcgaccac gcgcataccg
      961 cgaccgccgc cgccgccaga ggctttgata atgacgggat aaccgacttt atcggcgatt
     1021 ttgaggattt cttcgccgtc gtcgggcaat gcgccgtcag aaccggggac gcagggtacg
     1081 cctgccgcta tcatcgcgtg tttggcggag actttgtcgc ccatcaggcg gatggtgtcg
     1141 ggtttcgggc cgataaaggt aaagccggac tgctcgacct gttcggcgaa atcggcgttt
     1201 tcggcaagga aaccgtagcc cggatggaca gcgtccgcgc agcttacttc ggcggcggcg
     1261 ataatggcgg ggacgttaag gtagctttgc gcggaagcgg cagggccgat gcacacggat
     1321 tcgtcggcga gtttgacgtg caggctgtct ttgtcggcct cggaatgcac ggcgacggtg
     1381 gcaatgccca tttcgcggca ggcacggagt acgcgtaatg cgatttcgcc tcggttggcg
     1441 attaaaactt ttttcagcat gatgactgtt cctgtggttc cggcaacccg tccgtaaaaa
     1501 aaaaggtgcg ggaacgccga agggggaaag catttcagac ggcatcggaa gtttatgccg
     1561 tctgaaaaca ggattatccg atgatgaaga gcggttcgcc gaattcgacg ggcgtaccgt
     1621 tttcgaccag aatttctttg accgtgccgg atttttcggc ttcgatttcg ttcatcagct
     1681 tcatcgcttc gatgatgcac agcgtgtcgc cggcttttac ctgtttacct acttcaacga
     1741 aagcggctgc gttagggccg ggtgcgcggt agaacgtgcc gaccataggc gatttttgcg
     1801 cgtcggacaa atcgcgggcg gcaggtgcgg cgggcgcgga tgccgcaacg ggtgcggcgg
     1861 caggcgttac ggccggcgcg gcggcaggta cgggcgcggc gtaaacgggt gcggcggcga
     1921 tggttcgggt gatgcggact ttttcctcgc cttcggttac ttcgatttcg gcgatacccg
     1981 attcttcaac caaatcaatc agttttttta atttgcgcaa atccatttct gttcctttaa
     2041 aggctgccgg tgttccggcg ggttgttgcg ttggtttttc agaaaacagc cggcttgcgg
     2101 caacggcgca aaccgggcgg aatggtgttc ggatacggtt ttcctgtggt ggaaaacaac
     2161 cgtgagaatg tgtttatttt cccgaagttg tcggtaaatg tccagtaaaa tatcaaaaaa
     2221 cggcggtttt tgggggaaat gtgcaggaag cttgattttg cgcacaagat gccccgggtc
     2281 aagagtgttt attctaatct gttggttttt cggacaaaga tgccgtctga aaaagggcta
     2341 aagtgcgtat aatggcggct tgcccgaacg agagtgtaaa aatggatatt tcagattttg
     2401 attttaccct gcccgaaaag ctgattgccc agcatccgcc cgaagtgcgc ggcagcagcc
     2461 gccttttggt tgcgttgccc gatatgccgc tgcaagaccg ggtgttcggc gatttgccgg
     2521 attatgtcga ggcgggcgac gttttggtgt tcaacaacac caaagtcatg aaggcgcggc
     2581 tgtttgggca gaaagacagc ggcggcagga tcgaagccct gattgagcgc gtgttggaca
     2641 accataccgc actggcgcac atccgttcgt ccaagtcccc caagcccggt atggggctgg
     2701 tgtttgaagg cggcatccgt gccgtgatgg tcgggcgtga gggcgaactg ttctgcctgc
     2761 gttttgaagg cggtcaaacc gtttacgaac ttttggaaca gaacggccat ttgcctctgc
     2821 cgccttatat cgaacgtgcc gccgacgcgg acgacgacag ccgttatcaa accgtttatg
     2881 ccaaatatca gggcgcggtc gccgcgccga cggcgggcct gcattttacg gaagaacttt
     2941 tgcaccgtct gaaagacaaa ggcgcggtaa ccgcagaagt aactctgcac gtcggtgcgg
     3001 ggacattcca acccgtgcgt gtggaaaaaa tcgaagaaca caaaatgcac agcgaatggt
     3061 ttgaagtgcc gtctgaaacc gccgccgccg ttgaggcggc aaaagcccgg gggaacaaag
     3121 tctgggcggt cggcacgact tccatgcgcg ccctcgaatc tgccgcgcgt gcgacgggtc
     3181 gtctgaaagc ggggcagggc gacaccgata ttttcatcac gccgggctac cgttttaatg
     3241 ttgccgacag gctggttacc aatttccatc tgccgaaatc gacgctgctg atgcttgtca
     3301 gcgcgttttc gggtatgggt catatccgcg ccgcgtatcg ttatgcggtc gaacgcgaat
     3361 accgtttctt cagctacggc gatgcgatgg ttttggggcg gaacgaaggg gtggtacgtt
     3421 agaacagcct caagtctatt gggggaagac aagtgccgtc tgaacggatg ttcggacggc
     3481 attcttttgt tgatttgaat gaaaggaact gcatgacata tagtggatta aatttaaatc
     3541 aggacaaggc aacgaagccg tagacagtac aaatagtacg gcaaggcgag gcaacgctgt
     3601 actggttttt gttaatccac tatagattca ggcgcgagag gcgagaaact cagtcattgt
     3661 tgatgacgcg caatacgtta aaattcattc cgttttggtc gtataaacat attacatacg
     3721 ccattttttt gtattcaagg ctgcctgaaa acagtgggca agaatatcta acccaacgaa
     3781 aagctgaaaa agcaacaaaa aataatctgc accttagtcg gttggttagt ccaaccttag
     3841 gggtgcagac cattcaatct ttcaggctgc ctttgtttgt ttataagata aatttttagc
     3901 cgaacacttt ttccaagtgt gcctgataat ctgccaagta tttttccact tg
//
LOCUS       DAXHTR010000160         3890 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.160, whole genome shotgun sequence.
ACCESSION   DAXHTR010000160 DAXHTR010000000
VERSION     DAXHTR010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3890
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.160"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(1178..1615)
                     /gene="rnhA"
                     /locus_tag="ACFNML_001838"
     CDS             complement(1178..1615)
                     /gene="rnhA"
                     /locus_tag="ACFNML_001838"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFC6393157.1"
                     /translation="MDQTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
     gene            1729..2598
                     /gene="tehB"
                     /locus_tag="ACFNML_001839"
     CDS             1729..2598
                     /gene="tehB"
                     /locus_tag="ACFNML_001839"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HFC6393158.1"
                     /translation="MKERIVGQSGELFCFGQMPVWKAENLPEILLSGYSSEGGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKLKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAARDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAESLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPEVIADMQAHTAVG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELVKYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            complement(2648..3454)
                     /gene="thiD"
                     /locus_tag="ACFNML_001840"
     CDS             complement(2648..3454)
                     /gene="thiD"
                     /locus_tag="ACFNML_001840"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFC6393159.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETIFAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSTSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVQTAKAYITAAISNPLEIGAGHGPVNHWAYRD"
BASE COUNT          830 a          966 c         1098 g          996 t
ORIGIN      
        1 aatttaatcc actatagttt aatttgtctg tccgcattgc cgctaattgt accacttcat
       61 cgtatttcct tttcggttga aaccccgcca ctcggacatc tgtccttcgg ggcggcagga
      121 tcagacttta tttgggaggg ggtgtaaccc cttccaaatc aggacgacac atagggaggt
      181 gctttatgtg tcgtcctgtg tgttgaaata tatcggacgc gtttgaacag ttgtgcggca
      241 aactgtttta gcggcaggca agagatgccg tctgatgccg tctgaagccc attgtccgtc
      301 attcccacac aggcagttgc tacggttgct gaagcccctt agccgtcgcg caggcggttg
      361 ctacggttac taaaatcccg tcattcccgc gcaggcggga atccagtccg ttcggtttca
      421 gttgtttctg ataaatcttg cagcgttggg tttttagatt cccgctttcg cgggaatgac
      481 ggggcggtcg gatgccgcct gaaactccgt cattcttgca acttttcgtc attcccgtga
      541 aaacgggaat ctagaacttc aaactttcag ataatctttg aatattgccg ttgcccgacg
      601 gtctagattc ccgcttgcgc gggaatgacg gtagagcggt ttcagttgct cccgataaat
      661 gccgcaatct caaatcccgt cattccctca aaaacagaaa aacaaaatca gaaacctaaa
      721 atcccgtcat tcccgcagct cttcgtcatt cccgtgaaag cgggaatcta gaccttttaa
      781 attttcacac ggcttttgaa tattgccgtt acccgaaggt ctggattccc gcctgcacgg
      841 gaatgacggc agagcggttt cagttgtttc taaataaatt tttgcagcgt tggattttta
      901 gattcccgct ttcgcgggaa tgacggggcg gtcggatgcc gcctgaagcc cgacaaagcc
      961 cccaatgccg tctgaaacct cgtcattcct ctgctgtccg caacttttcg tcattcccgc
     1021 aaaagcggga atctagagcc tctaaatttt cagacggctt ttgaatattg ccgttgcccg
     1081 acggtttgga ttcccgcctg cgcgggaatg acggtggagg acgatgccgt ctaaagccca
     1141 ttagcggttt cagacggcat ttttgccgga gcggcagtca ggaaaactgc gctgcgccac
     1201 ggtttgccaa atcgtcggcg cgttcgtttt ccgcgtgtcc cgcgtgtcct ttaacccaag
     1261 tccagctgac ttggtgctgc ccaaccagaa cgtcgagttc tttccacaag tcgtcgtttt
     1321 tgacgggctg tttggcggcg gttttccagc cgttgcgctt ccaaccgtgt atccagtttt
     1381 ccatgccgtt tttgacgtat tgcgagtcgg tgcagatgat gacggtgcag cggcgtttga
     1441 gcgatttcag tccttcgatg acggcggtca gctccatgcg gttgttggtg gtttgcgctt
     1501 cgccgccgaa aagttctttt tcgtggctgc cgtagcgcat caacacgccc cagccgcccg
     1561 cgccgggatt gcctttgcac gcgccgtcgg tgtaaaggta aacggtttgg tccattattg
     1621 tgcctttgtg tggtgtccgg cgtgattata agcggtttgt gcgccgtgcc gtctgaaaga
     1681 ttgcggcgga ttaaaatagg catataatgt gcaactttat ttttgaatat gaaggagcgg
     1741 atagtgggtc aaagcggcga gttgttttgt ttcggacaaa tgcccgtatg gaaggcggaa
     1801 aacctgccgg aaattttgtt gtcgggttat tcgtccgaag gaggggaatg ggtctgcctg
     1861 aatgtgttgc agggcgatgt cgaagtccgc acgcaggacg gggcggcaga ggtttggtcg
     1921 gcggaaagcg gcgattgcgt gtttgcgccg cagcaggtgt tttcggtcaa actgaaaacg
     1981 gacgatgccg aaatccgttt gtcgctgtat tgcgcggcgg cagactattt tcacaaaaaa
     2041 tacgggatga gtgccacgca ttctgcggtc gcggcggcac gggataccgt accggcgggc
     2101 agggcgttgg atatgggctg cggacagggg cgcaacgcgc tgtttctcgg tttgaagggg
     2161 tttgaagtta ctgcagtcga tcacaatccc gccgccttgg caaacgtggc ggagctggca
     2221 gaggcggaga gtttgaacgt ccgcacgctg gaatatgatt tgaacgccgc cgccttgcag
     2281 ggcgagtttg attatattgt ggcaacggtg gtgctgatgt tcctgatgcc gcagcgcgtg
     2341 cctgaagtga ttgccgatat gcaggcgcat acggcggtgg gcgggtacaa cttaatcgta
     2401 tcggcaatgg atacggcgga tttcccctgt ccgatgccgt tccccttcaa atttaaagag
     2461 ggagagctga aggattatta tcgggattgg gagctggtca aatataaaga agaattgggc
     2521 gcgatgcacg ccaaagacga aaacggcaat ccgatacggt ttaaatttgt aacgatgctg
     2581 gcgaaaaagc ctgaataatc ctcaaaaatg ccgtctgaac attttgtttc agacggcatt
     2641 tttacgtcta gtcccgatac gcccaatgat tgaccggccc gtgtcctgcg ccgatttcca
     2701 aagggtttga gattgccgcc gtgatgtatg ccttggcagt ctgtacggct ttgcaaacgt
     2761 ctaagccttt tgccaactcg gcggtaatgc aggcagaaaa cgtgcagccc gtgccgtgcg
     2821 tgtgggcggt tggaaagcgc gggctgtcca attccagcgt ttcattttgc gtaaacagcc
     2881 aatccgtgca gcgtccgctt gtgctgccgt tcaaatgtcc gcctttgata acgacatttt
     2941 tgacaccgta atcaagcagg atttttgccg cacgttccgc atctttacgg ttttcgatat
     3001 gcacgccggt cagggcttcc gcctcgggca ggttgggggt caatacatcc gtatcgggaa
     3061 gcagcaggcg cgtcagtgcc gcaacggcgg aatcctgcaa cagcggcgca ccgcctttgg
     3121 caatcatcac ggggtcgagt acgcgcctgc cgaagtcgca gtgtttcagt ttgtcggcaa
     3181 cgcattcgat gatttccgcc gtgccgagca ttcctatttt gtaggcgcgg atgtcaaagt
     3241 cttccctgat tgcttggatt tgtgcgaaga tggtttcggt cgggacgaga tgaaccgccg
     3301 acacgcccaa ggtattttgc gcggtaacgg cggtgatgac gcacgttccg aacacgccgc
     3361 gcatctgaaa cgttttcaaa tccgcctgaa tgcccgcacc gccgcccgaa tccgaaccgg
     3421 cgatggtaag cgtttgcaca aaagagcttt tcatttgttc ctccttaata ttgtgtccga
     3481 taaaaagggc taatgcctgt aaaaaagcac ggcgatgccg tctgaaaacc gcagcgcggg
     3541 gtttgtggtg cattatatgc cgtattgcag gacaaggcac agggcagggc ggcggggtgc
     3601 atcgtagcaa ctgtattttt gcccgacggg gtgaaaaata cagttgctac acccactgat
     3661 aatgaaaaaa gagagcaatg ccgtctgaac cgcgtgttgc ggcgagcata aaccggcacg
     3721 ctttattcgt cattcccaca aaaacagaaa cctaaaattc cgtcattcct acgaaagcgg
     3781 gaatccagtg tgttcggttt cagttgtttt tgggtttcgg gtgatttcca aatcatcatt
     3841 cccgcgcagg cgggaatcta gcccttgggg cagcggcaat atttataaag
//
LOCUS       DAXHTR010000161         3861 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.161, whole genome shotgun sequence.
ACCESSION   DAXHTR010000161 DAXHTR010000000
VERSION     DAXHTR010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.161"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            490..1446
                     /gene="thiL"
                     /locus_tag="ACFNML_001841"
     CDS             490..1446
                     /gene="thiL"
                     /locus_tag="ACFNML_001841"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFC6393160.1"
                     /translation="MKEFDFIKRYLQTGTDNDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGIAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDDGGRELELHSLGFDHFG"
     gene            1439..1924
                     /locus_tag="ACFNML_001842"
     CDS             1439..1924
                     /locus_tag="ACFNML_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFC6393161.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFNML_001843"
     CDS             complement(1984..3660)
                     /gene="ettA"
                     /locus_tag="ACFNML_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFC6393162.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGCFNFKGSDQSKITGQLSG
                     GERGRLHLAKTLLSGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGAKPKRIKYKPVTR"
BASE COUNT          791 a          949 c         1072 g         1049 t
ORIGIN      
        1 gaggcaacgc tgtactggtt taaatttaat ccactatatc ggttgaaact ctgattttaa
       61 ggcggtagga tgtgggtttg cccatagaaa gggaatcctt tctgtatcaa gccctgaaag
      121 ggataattca tacaaattca cgcctttccc cctcattggg aaatggatgg aatcgtgcca
      181 gatgtgtgcg gcactgtatg ccggatatgg ttttatcatc agcccttttc ggttgaaacc
      241 tcgccagttg cagcgattga gcctaatcgg tggcggaagt tgccgctttg cattcggggt
      301 ggcgtgcagt gcggtgcttt gatatgccgt ttgtgtgttg aaacagggtg gtcggtgcat
      361 acgggtacgg tatggccaaa gctaaaagtg aaatacgctg aaacactgaa tgagccgctt
      421 tattgtttgt acggcctttg ctgccttgct atgatttaaa ttggattcgc ccgccggata
      481 ttttgggata tgaaagaatt tgacttcatc aaacggtatt tgcaaacagg cacggataat
      541 gatgtcgtat tgggcatagg cgacgatgcg gcgattgtcc gcccgcgtga aggcttcgat
      601 ttgtgtttca gtgcggatat gcttttgaag gacaggcatt tttttgcaga tgtcaaacct
      661 gaagacttgg cttggaaggt tttggccgtc aatatttcag atatggcggc gatgggtgcg
      721 ataccgcgtt gggtgttgct gagcgcggct ttgcccgaat tggatgaggt atggctgaaa
      781 cggttttgcg gcagcttttt cggtttggca aaaaagtttg gcgtaacgtt aatcggcggc
      841 gatacgacca agggcgatat ggcgttcaat gtaaccatta tcggcgaatt gccgaagggt
      901 agggcgttgc ggcgtgatgc ggcggttgcg ggcgacgata tttgggtgtc ggggcgtgtc
      961 ggtatggcgg cggcggcttt gaactgccgt ctgaaacggt gtgtgttgcc ggatgatgtg
     1021 tttgccgaat gcgaacaaaa gctgctccgc cccgagccaa gggttgggct ggggcttgcg
     1081 ctgttgccgt ttgcccgggc ggcgcaggat gtttcagacg gcatcgcgca agatttgggg
     1141 catatcctga ccgcttccgg cgtgggggcg gaaatttggg ccgattcgct gccgtcttta
     1201 tccgtattga aagatatttt gccccgagcg caatggctgt cttatacttt ggcgggcggc
     1261 gacgattacg agctggtgtt taccgcgccg gaaagttgcc gcagccgcgt gcttgatgcg
     1321 gcagaacggt gcggcgtgcc ggtaacgcgc atcggcaaaa tcaacggagg atgccgtctg
     1381 aaagttttag atgacggcgg cagggaattg gaactacatt ctttaggatt cgatcatttt
     1441 ggctgatttt aaacctgact ttgcgtggct gttgaaacga ccgttgtgtt ttttggcttt
     1501 tggtttcggc agcgggctgg ctccgttcgc gccgggtaca tttggcactt tggcggcact
     1561 gcctttggca tttgtgctga ttttgctcgg catagacggg ctgctgctgg cttttttgtg
     1621 tatcgtgctg tttatgtggg gcatacgcat ttgcgcttat gcggaacgtg aaacgggtgt
     1681 cagcgaccat ggtgggattg tttgggacga gattgtcgcc atgctgtttg tgctggcgtt
     1741 tgtgccgttc aggtggacgt ggtggctggc ggcatttgtc ctattccgtc tgtttgacgc
     1801 gctcaaaccg tctcccgtcg gttggtttga caagaatctg cacggcggtt tgggcattat
     1861 ggcggacgat atggcggctg cggtgatgac tttgattgtc ttgaggattg caatgctgtt
     1921 ttaaacggtg ctgccttgta aaaatgccgc ctgaaagcct ttcagacggc attgtttcgg
     1981 aggttaacgc gttaccggtt tgtatttgat gcgtttcggt ttcgcgcctt cttcgcccaa
     2041 acggcgtttc ttatcggctt cgtattcctg atagttgccg tcgaagaaca cccatttgga
     2101 gtcgccttcg caagccaaga tatgcgtagc aatacggtcg aggaaccaac ggtcgtgaga
     2161 aatcaccatc acgctgccgg caaattccaa caatgcgtct tccagcgcgc gcagggtttc
     2221 cacatcgagg tcattagacg gttcatccag cagcaataca ttgccgccgc tcaacaaggt
     2281 tttcgccaag tgcaaacgtc cgcgttcgcc gccggaaagc tgccccgtga ttttgctttg
     2341 gtcgctgcct ttgaaattga agcatcccaa atattggcgg gcggggattt caaactgccc
     2401 gacctgtaaa atatcgcgac cttcggcaat gttgtcgaac acggttttgt cgttttgcaa
     2461 accttcgcgg ctttggtcaa tcaagctcat tttcacggtt tgcccgattt tcacttcacc
     2521 ggaatcgggc tgctctttgc ccgcaatcat tttgaacagc gtagatttac ccgcgccgtt
     2581 cggaccgatg atgccgacaa tcgcgcccgc aggcactttg aagctcaaat cgtcaatcag
     2641 cactttatcg ccgaacgatt tggaaacatt tacaaattca atcacttcgt tacccaaacg
     2701 ctcggcaacg ggaataaaga tttcctgcgt ttcattgcgt ttttggtatt cgtagttgct
     2761 catttcctca aaacgcgcca aacgcgcttt ggacttggct tggcggcctt tggcattttg
     2821 gcgcacccat tccaattcct gcttcatcgc cttcacgcgc gcggcttcgg attttgcctc
     2881 gttttccaag cgtttttctt tctgctccag ccaagacgag taattgcctt tccacggaat
     2941 accatggccg cggtcgagtt ccaaaatcca ttcggcggcg ttgtcgagga agtagcggtc
     3001 gtgcgttacc gcaacgactg tgccggggaa gcgcacgaga aattgctcca gccactcgac
     3061 cgattccgca tccaagtggt tggtcggctc gtccagcaaa agcatatcgg gcttgctcaa
     3121 caagagtttg cacaaggcaa cgcggcgttt ttcaccgccg gacaaattat cgattttggc
     3181 atcccattcc ggcaggcgca gcgcgtcggc agcgatttcc aattcatgct ctgcaccgcc
     3241 gcccgtggac gaaccggccg cgataatcgc ttccaagcgg ccctgctctt ccgccaacgc
     3301 gtcaaaatcc gcatcgggat tggcatactc ggcatacact tcttccaagc gtttctgcgc
     3361 cgccgccact tcgcccaaac cgctttccac ttcctcgcgc acggtttttt caggatcaag
     3421 ctcaggctct tgcggcaggt agccgatttt gatgccgccc atcggcacgg cttcgccctc
     3481 aaattcctta tccacgcccg ccataatccg cagcacggtg gacttgcccg cgccgttcaa
     3541 accgagcagg ccgattttcg cgccggggaa gaaagaaagg gaaatatctt taataatggt
     3601 tttctgcggc ggcacaacct tgctcacgcg cagcatagaa tagacgtatt gttgggacat
     3661 ggttttctcg ttttcatcaa acaaatttca gacggccatt ttaaccgata atttgattta
     3721 agccagttta tccgcgaacc ggtattgcca aaatcgggca ggattcataa aatccgctta
     3781 tccctttgaa attatataga caaaaaaata ataatgatag gggatcgccg ccccggcaac
     3841 catttcggat tttccaaagc a
//
LOCUS       DAXHTR010000162         3812 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.162, whole genome shotgun sequence.
ACCESSION   DAXHTR010000162 DAXHTR010000000
VERSION     DAXHTR010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.162"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            362..3568
                     /gene="nalP"
                     /locus_tag="ACFNML_001844"
     CDS             362..3568
                     /gene="nalP"
                     /locus_tag="ACFNML_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HFC6393163.1"
                     /translation="MRTTPTFPTKTFKPAAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLH
                     TGDFTNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYN
                     ENYKNYTAYMRKEAPEDGDGKDIEASFDDEVVIETEAKPTDIRHVKEIGHIDVVSHII
                     GGRSVDGRPAGGIAPDATLHIMNTHDGTKNEIMSAAIRNAWVKLGERGVRIVNNSFGT
                     TSRAGTADHFQIANSEEQYRQALLAYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLF
                     IFSASNDAQAQPNTLTLLPFYEKDAQKGIITVAGVDRSGEKFNGSNHCGITAMWCLSA
                     PYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDNLRTTLLTTAQDIGA
                     VGVDSKFGWGLLDTGKAMNGPASFPFGDFTADTKGTSDIAYSFRNDISGTGGLIKKGG
                     SQLQLHGNNTYTGKTVIEGGSLVLYGNNKSDMHVETKGALIYNGAASGGSLNSDGIVY
                     LADTDRSGANETVHIKGDLQLGGEGTLYTRLGKLLKVDGTAMTGGKLYMSARGKGAGY
                     LNRTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAGSEGDTLSYYVRRGN
                     AARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPETVETAAADRTDMPG
                     IRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADMQGRRLKAVSDGLDH
                     NATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTGENTTAAATLGMGHSTWS
                     ENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRSTGADEHAEGSVNGTL
                     MQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGWSGNSLTEGTLVGLA
                     GLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGKTGARNMPHTRLVAG
                     LGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1031 a         1033 c         1015 g          733 t
ORIGIN      
        1 agccgcagac agtacggaac cgattcactt gttaaagaat cgttctcttt gagctaaggc
       61 gaggcaacgc cgtactggtt tttgttcatc cactataact agggaaattc aaattaagtt
      121 agaattatcc ctatgagaaa aagccgtcta agccggtata aacaaaataa actcattgag
      181 ctatttgtcg aaagttcaaa tttccatttt aaaacaatta gtaaaatcga gtttatccta
      241 gttgtccaag acaaccccta taataatata attcaaaata taaaaacggg ttacatctaa
      301 acattacgga atttttattc cctcgcctga attctattgt cagattcaag gagacctcat
      361 catgcgaacg accccaacct tccctacaaa aactttcaaa ccggctgcca tggcgttagc
      421 tgttgcaaca acactttctg cctgcttagg cggcggcgga ggcggcactt ctgcgcccga
      481 cttcaatgca ggcggtaccg gtatcggcag caacagcagg gcaacaacag cgaaatcagc
      541 agcagtatct tacgccggta tcaagaacga aatgtgcaaa gacagaagca tgctctgtgc
      601 cggtcgggat gacgttgcgg ttacagacag ggatgccaaa atcaatgccc cccccccgaa
      661 tctgcatacc ggagacttta caaacccaaa tgacgcatac aagaatttga tcaacctcaa
      721 acctgcaatt gaagcaggct atacaggacg cggggtagag gtaggtatcg tcgacacagg
      781 cgaatccgtc ggcagcatat cctttcccga actgtatggc agaaaagaac acggctataa
      841 cgaaaattac aaaaactata cggcgtatat gcggaaggaa gcgcctgaag acggagacgg
      901 taaagacatt gaagcttctt tcgacgatga ggtcgttata gagactgaag caaagccgac
      961 ggatatccgc cacgtaaaag aaatcggaca catcgatgtg gtctcccata ttattggcgg
     1021 gcgttccgtg gacggcagac ctgcaggcgg tattgcgccc gatgcgacgc tacacataat
     1081 gaatacgcat gatggaacca agaacgaaat aatgtctgca gccatccgca atgcatgggt
     1141 caagctgggc gaacgtggcg tgcgcatcgt caataacagt tttggaacaa catcgagggc
     1201 aggcactgcc gaccatttcc aaatagccaa ttcggaggag cagtaccgcc aagcgttgct
     1261 cgcctattcc ggcggtgata aaacagacga gggtatccgc ctgatgcaac agagcgatta
     1321 cggcaacttg tcctaccaca tccgtaataa aaacatgctt ttcatttttt cggcaagcaa
     1381 tgacgcacaa gctcagccca acacactgac cctattgcca ttttatgaaa aagacgctca
     1441 aaaaggcatt atcacagttg caggcgtaga ccgcagtgga gaaaagttca atggctccaa
     1501 ccattgcgga attactgcca tgtggtgcct gtcggcaccc tatgaagcaa gcgtccgttt
     1561 cacccgtaca aacccgattc aaattgccgg aacatccttt tccgcaccca tcgtaaccgg
     1621 cacggcggct ctgctgctgc agaaataccc gtggatgagc aacgacaacc tgcgtaccac
     1681 gctgctgaca acggctcagg acatcggtgc agtcggcgtg gacagcaagt tcggctgggg
     1741 actgctggat acgggtaagg ccatgaacgg acccgcgtcc tttccgttcg gcgactttac
     1801 cgccgatacg aaaggtacat ccgatattgc ctactccttc cgtaacgaca tttcaggcac
     1861 gggcggcctg atcaaaaaag gcggcagcca actgcaactg cacggcaaca acacctatac
     1921 gggcaaaacc gttatcgaag gcggttcgct ggtgttgtac ggcaacaaca aatcggatat
     1981 gcacgtcgaa accaaaggtg cgctgattta taacggggcg gcatccggcg gtagcctgaa
     2041 cagcgacggc attgtctatc tggcagatac cgaccgatcc ggcgcaaacg aaaccgtgca
     2101 catcaaaggc gatctgcagc tgggcggcga aggtacgctg tacacacgtt tgggcaaact
     2161 gctgaaagtg gacggtacgg cgatgaccgg cggcaagctg tacatgtcgg cacgcggcaa
     2221 aggggcaggc tatctcaacc gtaccggaca acgtgttccc ttcctgagtg ccgccaaaat
     2281 cgggcgggat tattctttct tcacaaacat cgaaaccgac ggtggtctgc tggcttccct
     2341 cgacagcgtc gaaaaaacag cgggcagtga aggcgacacg ctgtcctatt atgtccgtcg
     2401 cggcaatgcg gcacggactg cttcggcagc ggcacattcc gcgcccgccg gtctgaaaca
     2461 cgccgtagaa cagggcggca gcaatctgga aaacctgatg gtcgaactgg atgcctccga
     2521 atcatccgca acacccgaga cggttgaaac tgcggccgcc gaccgcacag atatgccggg
     2581 catccgcccc tacggcgcaa ctttccgcgc agcggcagcc gtacagcatg cgaatgccgc
     2641 cgacggtgta cgcatcttca acagtctcgc cgctaccgtc tatgccgaca gtaccgccgc
     2701 ccatgccgat atgcagggac gccggctgaa agccgtatcg gacgggttgg accacaacgc
     2761 tacgggtctg cgcgtcatcg cgcaaaccca acaggacggt ggaacgtggg aacagggcgg
     2821 tgttgaaggc aaaatgcgcg gcagtaccca aaccgtcggc attgccgcga aaaccggcga
     2881 aaatacgaca gcagccgcca cactgggcat gggacacagc acatggagcg aaaacagtgc
     2941 aaatgcaaaa accgacagca ttagtctgtt tgcaggcata cggcacgatg cgggcgatat
     3001 cggctatctc aaaggcctgt tctcctacgg acgctacaaa aacagcatca gccgcagcac
     3061 cggtgcggac gaacatgcgg aaggcagcgt caacggcacg ctgatgcagc tgggcgcact
     3121 gggcggtgtc aacgttccgt ttgccgcaac gggagatttg acggtcgaag gcggtctgcg
     3181 ctacgacctg ctcaaacagg atgcattcgc cgaaaaaggc agtgctttgg gctggagcgg
     3241 caacagcctc actgaaggca cactggtcgg actcgcgggt ctgaagctgt cgcaaccctt
     3301 gagcgataaa gccgtcctgt ttgcaacggc gggcgtggaa cgcgacctga acggacgcga
     3361 ctacacggta acgggcggct ttaccggcgc gactgcagca accggcaaga cgggggcacg
     3421 caatatgccg cacacccgcc tggttgccgg tctgggcgcg gatgtcgaat tcggcaacgg
     3481 ctggaacggc ttggcacgtt acagctacgc cggttccaaa cagtacggca accacagcgg
     3541 acgagtcggc gtaggctacc ggttctgacg gacaggaagc agacagccgc aaagatcacg
     3601 gtctttgcgg ctgtttctta tgaaaagaaa accctattcc aattgcctgc ttctattgtt
     3661 tcaagacttc ttccaaagat tcggcattaa tcagatgtat agcggattaa caaaaatcag
     3721 gacaaggcgg cgaagccgcg gacagtacaa atagtacgga accgattcac tcggtgcttg
     3781 agcaccttag agaatcgttt ctttgagcta ag
//
LOCUS       DAXHTR010000163         3671 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.163, whole genome shotgun sequence.
ACCESSION   DAXHTR010000163 DAXHTR010000000
VERSION     DAXHTR010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.163"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..328
                     /locus_tag="ACFNML_001845"
     CDS             <1..328
                     /locus_tag="ACFNML_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HFC6393164.1"
                     /translation="TEEGIAQTVVKGKTTRAEVEARFGKRNPFGCYAYHEVSLPIYNF
                     LPTNFIYMKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVIRKEAGKS
                     LSQPEK"
     gene            392..1675
                     /gene="hemL"
                     /locus_tag="ACFNML_001846"
     CDS             392..1675
                     /gene="hemL"
                     /locus_tag="ACFNML_001846"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFC6393165.1"
                     /translation="MNRNEILFDRAKAVIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVVEAVREAALGGLSFGAPTEGEIVIAEQIAEI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGITPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIRREGFYENLTARTEQLVQG
                     FQTAADAAGIEFTADSVGGMFGLYFASHTPQNYGDMARSNIEGFKQFFHGMLDRGIAF
                     GPSAYEAGFVSAAHTPELIDETVAVAVEVFKAMAA"
     gene            complement(1891..2163)
                     /locus_tag="ACFNML_001847"
     CDS             complement(1891..2163)
                     /locus_tag="ACFNML_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HFC6393166.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2174..2737)
                     /gene="orn"
                     /locus_tag="ACFNML_001848"
     CDS             complement(2174..2737)
                     /gene="orn"
                     /locus_tag="ACFNML_001848"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFC6393167.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAIHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPERATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2755..3642)
                     /gene="prmA"
                     /locus_tag="ACFNML_001849"
     CDS             complement(2755..3642)
                     /gene="prmA"
                     /locus_tag="ACFNML_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFC6393168.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQIAAQECGLKDLAYTGETIEDQDWVR
                     LTQSQFDPIRISDRLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQIEELSGIYSQWF
                     DLDPAETEEGWARLSGTKR"
BASE COUNT          772 a          943 c         1059 g          897 t
ORIGIN      
        1 gacggaagag ggaatcgcac aaacggttgt taaaggcaaa acgacccggg cggaggtaga
       61 ggcacgtttc gggaagcgca accctttcgg ttgttatgcc tatcacgagg tcagcctgcc
      121 gatttataat tttttgccga ccaatttcat ttatatgaaa tcagaacgcc gacattggga
      181 gtggtgtgtg gattacgact gggaaggagt tgtccgggac taccgcttta cacataaagt
      241 ggaaaaagac gagcgttccg tcatccggga tgcggttggc gtcatccgca aagaagcagg
      301 caaatccttg tcgcaacctg aaaaatgata aaatgggact ttctgcttct aagcccgaaa
      361 cctgccgttc agacggcatt tgaggataaa tatgaaccgt aacgaaattt tattcgaccg
      421 cgccaaggcg gtcatccccg gcggcgtgaa ttcccccgtg cgcgcattcg gcagcgtcgg
      481 cggcgtaccg cgcttcatca aaaaagccga aggcgcgtat gtttgggatg aaaacggcac
      541 gcgctacacc gactatgtcg gctcttgggg ccctgcgatt gtcggacacg cgcaccccga
      601 agtcgtcgaa gccgtgcgcg aagccgcgtt gggcggtttg tcattcggtg cgcccaccga
      661 gggcgaaatc gtcatcgccg aacaaatcgc cgaaattatg ccgtctgtcg aacggctgcg
      721 cctcgtcagc tccggcacgg aagcgacgat gaccgccatc cgtctggcac gcggttttac
      781 cggccgcgac aaaatcatca agtttgaagg ctgctaccac gggcattccg acagcctgct
      841 ggtgaaagcc ggcagcggcc tgctgacgtt cggcaatcct tcttccgccg gtgtgcctgc
      901 cgactttacc aaacacacct tagtcctcga atacaacaac atcgcccaat tggaagaagc
      961 ctttgctcaa agcggcaatg acattgcctg tgtgattttg gagccttttg tcggcaatat
     1021 gaaccttgtc cgcccgaccg aagccttcgt caaagccctg cgcgaattga ctgaaaaaca
     1081 cggcgcggtg ttgatttacg acgaagtgat gacaggtttc cgcgtcgcac tcggtggcgc
     1141 acaatcgctg cacggcatca cgcccgacct gaccacgatg ggcaaagtca tcggcggcgg
     1201 tatgccgctt gccgcgttcg gcggacgcaa agacattatg gaatgtattt ccccactggg
     1261 cggcgtgtat caggcaggta cattatcagg caacccgatt gccgtcgccg ccggtttgaa
     1321 aacgctggaa atcatccgcc gcgaagggtt ttatgaaaac ctgacggcgc gtaccgaaca
     1381 gttggttcaa ggttttcaga cggcagcgga tgcggcgggc atcgagttca ccgccgacag
     1441 cgtgggcggt atgtttggtt tgtattttgc cagccatacg ccgcaaaact acggcgacat
     1501 ggcgcgttcc aatattgaag gcttcaaaca gtttttccac ggcatgctcg accgcggcat
     1561 tgccttcggc ccgtccgctt atgaagcagg tttcgtttct gccgcgcata cgcccgagct
     1621 gattgatgaa acggttgcgg ttgcggttga agtgttcaag gcgatggctg catgatgttt
     1681 tgacggacag ggtttccctg ttcaatttgt ttggcagatt gaagtaagaa tgcacaccgc
     1741 cgtcattccc gcgcaggcgg gaatccagac ctttcagttt ctgtaatgat tgaaaataac
     1801 gtgccgaaat cagccgcacc cggcaggaag ccgccgccac ctgaaacaat gccgtctgaa
     1861 gccttcagac ggcattttat cggaagcgca tcagaaaaaa cggatagcga catatgcggt
     1921 aaaagcaagg gcaattacat agataaaccc ctttataaat tcaaaagtct catctatatc
     1981 gctatatttt tctttcagtt cgtcagattt tcgataacgt ttcattttca agaccacttc
     2041 atgaccaaca agcccgacct tccggattcc cgcctgcgcg ggaatgacgg gcgtgtatat
     2101 ttttgatttc aatctgctgt aaaaatgccg tctgaaatgc aggatttgcg cttcagacgg
     2161 cattgtttgt ttcttattgc gcttcggctc tcggggcgga aatcagaaag tgttcgcggt
     2221 agtggcgcat ttcttcgatg ctctccaaaa tgtcgtccaa tgccttgtgc gaaccgcgtt
     2281 tgaccacgct tttggcaacg ggcggattcc agcgtttggc aagctctttg agcgtggaaa
     2341 cgtcgaggtt gcggtagtgg aagtagtttt ccagtttcgg catatatttg accataaaac
     2401 gccggtcttg gtggatggag ttgccgcaca tcggcgtggc gcgttcgggt acccattccg
     2461 acataaagtc cagcagtttc tgttcgactt cggcttcggt atgcgacgat tcgcgtacgc
     2521 gctgcgtcag ccccgtccta ccgtgtgtgg cggtgttcca ctcgtccata ttgtcgagca
     2581 gctcgtcgct ttggtggatg gcgtaaactt cggattgcgc caatacattc aaatccgagt
     2641 cggtaataat catcgcgact tcgataatgc ggtcggtttc gggattaagc cccgtcattt
     2701 ccatatcgag ccagcatagg ttgtttttat cctgcatgat gtttcctttg cttgtcagcg
     2761 ttttgtcccg cttaatcgcg cccatccttc ttcggtttcc gcaggatcga gatcgaacca
     2821 ttggctgtaa atgccgctga gttcttcgat ttgttcgtcc aacaaaccgg ataacacgat
     2881 gcggccgcct tgtttggtgc gggcggcaag catttcgccg agcatacgta aagggttggc
     2941 gaggatgttg gcaacaacta cgtcgaattg cccttgaggc aggccgtcgg gcaggaagaa
     3001 ttgtgcatcg acgttgttct gttcggcgtt gtccttgccg gcgcggacgg cttgttcgtc
     3061 aatatcgacg ccgacggcag aacctgcgcc gagtttgagg gcagcgatgg tcaggatgcc
     3121 cgaaccgcag ccgtagtcga ggacgctttc gccgtttttg agttgcgtat ccagccattt
     3181 gaggcagagg cgcgtggttg ggtggctgcc agtgccgaag gcgaggccgg gatcgaggcg
     3241 taggttgacg gcagtgcctt cgggggcttc gtgccaagag ggggtaatcc acaggcggtc
     3301 ggaaattcgg atggggtcga attgcgattg cgtgagacgc acccagtctt ggtcttcgat
     3361 ggtttcgccg gtgtatgcca agtcttttaa cccgcattct tgtgcggcga tttggatgat
     3421 ggcggcggct tcgtcgtgtt cgccgaacag ggcgatgact ttgctctgct gccagatttg
     3481 ttcggcgggc ataccgggtt cgccgaaaat cgcctgttcg ttttgcgtgc cggcgtaggc
     3541 atcttcgatg gcggcggaga gtgcgccgtg ttccatcagc gcgtcggcga ggcgttcggc
     3601 gacggcatcg ttgacgttga cggtgatttg ttggtagggc ataacgggct ttcttggaaa
     3661 tatagtggat t
//
LOCUS       DAXHTR010000164         3601 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.164, whole genome shotgun sequence.
ACCESSION   DAXHTR010000164 DAXHTR010000000
VERSION     DAXHTR010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.164"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            322..1794
                     /gene="pyk"
                     /locus_tag="ACFNML_001850"
     CDS             322..1794
                     /gene="pyk"
                     /locus_tag="ACFNML_001850"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFC6393169.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
     gene            2102..3502
                     /locus_tag="ACFNML_001851"
     CDS             2102..3502
                     /locus_tag="ACFNML_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HFC6393170.1"
                     /translation="MTPSALKKTVLLLGTAFAAASAQASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIKSKAEILTAKPPKPNGVAEAAKIQAD
                     GHADVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADGAAAKAMWS
                     TMLAANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFE
                     KEKTIVNGKSDRTTITPNWRNTYKVGFGGSYQISEPLQLRAGIAFDKSPVRNADYRMN
                     SLPDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTSYRTAKASGNDVDSKGASSARF
                     KNHADIIGLQYTYKFK"
BASE COUNT          934 a         1072 c          907 g          688 t
ORIGIN      
        1 cctgcgacaa atagctcaat gagtttattt tgtttatact ggcttagacg actttttctc
       61 atagggataa ttctaactta atttgaattt ccctagttat ctaggacagc cccaaaataa
      121 atttagccta agtcataaaa ccgtgtaatt ttgcgccaaa aaaggcggtt tgcaggtaaa
      181 atatcaaact attaatttaa atcatatttt tacagaatat tccgccgcgt tcatcaaatg
      241 gacatcaaac cggttccaaa tttctgtaat tttgtaacaa aataccgcaa aacacccgat
      301 tgagaccaaa aggactttca tatgaaccag acaagccgcg atctgacccg catcagccac
      361 aacactaaaa tcgtcgccac ccttgggccg ggcagcaaca acgtagaact gttggaagat
      421 atgatccgcg tcggcggcct gaacgtcgtc cgcttcaact tcagccacgg cacgcccgaa
      481 ttccatcagg aaaacgcccg catcgtgcgc gaggcggcaa aacgcgccgg acaggaaatc
      541 gccatcattg ccgacctgca agggccgaaa atccgcgtgg gcaaaatcgc cggcggcggc
      601 attgaattga acaaaggcga aacgctggta ctggatgccg cgctcgaagg cgaaggcacg
      661 cgcgaggcgg tcggtttgga ctaccgcgac ctgcccgacg acgttgccgc aggcgacgta
      721 ttgtggctgg acgacggcct gctgaccctg accgtggaat ccgtcgaagg cagcaggatt
      781 atcacaaggg tggaaaacag ccatgtcctg aaaagcaaca aaggcatcaa caaacgcggc
      841 ggcgggctgt ccgcaggcgc gttgaccgaa aaagacttcc gcgacctgaa aaccgcgatt
      901 gccatcggtt gcgactacct cgccatcagc tttgtgaaat ctgccgaaga tttgcacatc
      961 gcgcgcgcca aagtcgaaga agaaatgaag ggcagcactg ccgtgcgccc cggtttggtt
     1021 tccaaaatcg aacgcgtaga agcgattgaa aaccttgatg aaatcatcct cgccggcgac
     1081 ggcattatgg ttgcgcgcgg cgacttggcg gtcgaagtcg gacacgccgc cgtccccgcc
     1141 ctgcaaaaac ggatgatccg ccgcgcccgc gagttgcgcc gcttcagcat tacggcgacg
     1201 caaatgatgg aatcgatgat taccaacccc gtgccgaccc gcgcggaagt cagcgatgtg
     1261 gcaaacgcgg tattggacgg taccgatgcg gtgatgtgtt ccgccgaaac cgccgtcggc
     1321 gcgtatccgt ttgaaaccgt cagccaaatg gcaattatct gcgcggctgc ggaaaaagag
     1381 caggattcgc tcaacggcgt tgccgaacag gcggagtatc ccgaagcggt cagcaccaac
     1441 ctggcggttg ccggcggcgc ggtcagcgtg gcgcgcgcgg ttcacgccaa agcgattgtc
     1501 gccctgaccg aaagcggttc gaccgccttt gaaatcagcc gccacaacat caccctgccg
     1561 attttcgcgc tgaccccgag cgtttccgcc caacgccgta tggcgatgta ccgcggcgtg
     1621 cgcccgctga ttttggcaac cagtaccgac cacgacacgg cgttgaacga agtcgaaacc
     1681 atgctggtgg aacacaacat cctgcattcc ggcgaccagt acatcatcac cagcggttcg
     1741 caaatgcgcg aatccggttc gaccaacaca ctggaagtgc tgcgcgtcaa ataatccgcc
     1801 ctgagtggaa aatgccgtct gaagccgatg cccgaggctt cagacggcat tttttgcggc
     1861 ggcgcggcgg ttcgggcaaa ctgaaaaaat tgtgcaaatc cggcaacatc ggataaaatt
     1921 gagtacctat actaaagcga aacaaggcat ttccgactgc cttttttata gtggattaaa
     1981 tttaaaccag tacggcgttg ccttgccgta ctatctgtac tgtctgcggc ttcgtcgcct
     2041 tgtcctgatt tttgttaatc cactatattt atccaccgtc cgccttttta cggaaaccga
     2101 aatgacccct tccgcactga aaaaaaccgt cctactgctc ggcactgcct ttgccgccgc
     2161 atccgcacaa gcctccggct accacttcgg cacacagtcg gtcaacgcgc aaagcacggc
     2221 aaatgccgcc gccgcagaag ccgccgacgc atcgaccatc ttctacaacc ctgccggcct
     2281 gaccaaactc gacagcagcc agatttccgt caacgccaac atcgtgctgc ccagcattca
     2341 ttatgaggcg gattccgcca ccgactttac cgggcttccc gtccaaggtt cgaaaagcgg
     2401 caaaatcacc aaaaccacgg tcgcgcccca catctacggc gcatacaaag tcaacgacaa
     2461 tctgaccgtg ggcttgggcg tgtacgtccc cttcggctct gccaccgaat acgaaaaaga
     2521 ttccgtgttg cgccacaaca tcaacaaact cggtctgacc agcatcgccg tcgaacctgt
     2581 cgccgcatgg aaactcaacg accgccattc cttcggcgca ggcatcatcg cccaacatac
     2641 ttccgccgaa ctgcgcaaat atgccgactg ggggattaag agtaaagcag agatattgac
     2701 ggcaaaaccg cccaaaccta acggtgtagc cgaagctgca aaaattcagg ccgacggaca
     2761 cgccgatgtc aaaggcagcg attggggctt cggctaccaa ctggcgtgga tgtgggacat
     2821 caacgaccgc gcgcgcgtgg gcgtgaacta ccgttccaaa gtctcgcaca cgctcaaagg
     2881 cgatgccgaa tgggcggcag acggcgcggc ggcgaaagca atgtggagta cgatgcttgc
     2941 agcaaacggc tacacggcga atgaaaaagc ccgcgttaaa atcgttacgc ctgagtcttt
     3001 gtccgtacac ggtatgtaca aagtgtccga taaagccgac ctgttcggcg acgtaacttg
     3061 gacgcgccac agccgcttcg ataaggcgga actggttttt gaaaaagaaa aaaccatcgt
     3121 caacggcaaa tccgaccgca ccaccatcac ccccaactgg cgcaacacct acaaagtcgg
     3181 cttcggcggt tcttatcaaa tcagcgaacc gctgcaactg cgcgccggca tcgcttttga
     3241 caaatcgccc gtccgcaacg ccgactaccg catgaacagc ctgcccgacg gcaaccgcat
     3301 ctggttctcc gccggcatga aataccatat cggcaaaaac cacgtcgtcg atgccgccta
     3361 cacccacatc cacatcaacg acaccagcta ccgcacggcg aaggcaagcg gcaacgatgt
     3421 ggacagcaaa ggcgcgtctt ccgcacgttt caaaaaccac gccgacatca tcggcctgca
     3481 atacacctac aaattcaaat aaacggcccg ccgtttgatt gtagaaatgc cgtctgaaac
     3541 agatttccgt ttcagacggc attgttttag gcgcgattta tagtggatta acaaaaatca
     3601 g
//
LOCUS       DAXHTR010000165         3597 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.165, whole genome shotgun sequence.
ACCESSION   DAXHTR010000165 DAXHTR010000000
VERSION     DAXHTR010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.165"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..412)
                     /locus_tag="ACFNML_001852"
     CDS             complement(<1..412)
                     /locus_tag="ACFNML_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HFC6393171.1"
                     /translation="MMHTPSPHNEFIRAIKESSPILIGLLPWALILGMQGGQKGMSWL
                     EMLLMTGMNFAGGSEFATVNLWAEPLPILLIATVTFMINSRHILMGAALALHLKEIPL
                     KKAVPALFFMCDESWAMAFSEIQKRKATGLPAFNM"
     gene            554..1006
                     /gene="dut"
                     /locus_tag="ACFNML_001853"
     CDS             554..1006
                     /gene="dut"
                     /locus_tag="ACFNML_001853"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFC6393172.1"
                     /translation="MNIEVEMKVLDERMADFIPTYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPSYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFAVKPFERIAQMVIVPIVQARFKRVEEFVGSSRGEGGFGSTGLH"
     gene            1083..2270
                     /gene="dapC"
                     /locus_tag="ACFNML_001854"
     CDS             1083..2270
                     /gene="dapC"
                     /locus_tag="ACFNML_001854"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009016 - succinyldiaminopimelate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HFC6393173.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGIKPVIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            2433..3212
                     /gene="yaaA"
                     /locus_tag="ACFNML_001855"
     CDS             2433..3212
                     /gene="yaaA"
                     /locus_tag="ACFNML_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFC6393174.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRELALQ
                     QIAELMHVSDKIALLNAQRNAEWHTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRAQLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            3295..3385
                     /locus_tag="ACFNML_001856"
     tRNA            3295..3385
                     /locus_tag="ACFNML_001856"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3329..3331,aa:Ser,seq:tga)
     gene            3463..3552
                     /locus_tag="ACFNML_001857"
     tRNA            3463..3552
                     /locus_tag="ACFNML_001857"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3497..3499,aa:Ser,seq:cga)
BASE COUNT          920 a          853 c          880 g          944 t
ORIGIN      
        1 gcatattgaa tgcgggtaaa ccggttgctt tccgtttttg gatttcggag aatgccatcg
       61 cccagctttc atcacacata aaaaacagtg cgggcacggc ttttttcagc ggtatttctt
      121 tcaggtgcag ggcaagtgcc gcccccatca ggatatgccg cgaattaatc ataaaggtta
      181 cggtggcgat aagcagtatc ggcagaggtt ccgcccacag gttgaccgtg gcaaactcgg
      241 agccgccggc gaagttcata ccggtcatca acaacatttc cagccagctc atgccttttt
      301 gtccaccctg cataccgagt attaatgccc aaggcagaag tccgataagt atgggcgaac
      361 tttctttgat ggcgcgtata aattcgttat gcggggaagg tgtgtgcata atgttcgtct
      421 tcataacggg aagggcgggc attatacact ggcaacggat ttcaaaacaa aaccgatttg
      481 ccgtgtttca gcgtaaacac ggcttgtgta taatctccca tctttgaaac cggccgtatg
      541 caggagcaag acgatgaata ttgaagtaga aatgaaagtg ttggacgaga ggatggcgga
      601 ttttatccct acctatgcga cagcgggttc tgccggtttg gacttgcgcg cctgtttgga
      661 tgaggaagtc gtcttgcagc cgggggaaac gtttcttgtg ccgacaggtt tggcaattta
      721 tttggccaat ccttcatatg ccgccgtttt gctgccccgt tccggcttgg ggcataaaca
      781 cggcattgtt ttgggcaatt tggtcggttt gattgactcc gattatcaag gggaattgaa
      841 ggtgtcgtta tggaacagaa gcagcgaacc gtttgccgtc aagccgtttg agcgtatcgc
      901 gcagatggtt atcgttccaa tcgtgcaggc gcgtttcaaa cgtgtcgaag aatttgtcgg
      961 aagcagccgt ggagaaggcg gtttcggcag tacgggtttg cattgaatat aaaatgccgt
     1021 ctgatgggtg ccgtcaggtt cagacggcat atcttccgat ataccgacaa ccggagaaca
     1081 ttatgaatac cttactcaac caactcaaac cctatccctt tgcccgactg cgtgaagcga
     1141 tgcagggcat ttccgccccc gaaggtctgg aagccgtccc actgcatatc ggcgaaccga
     1201 aacatccaac accgaaagtc attacagatg cgctgaccgc ctcattgcac gagttggaaa
     1261 aatatccgct gacggccggt ttgcctgaac tgcgtcaggc gtgtgcgaac tggttaaaac
     1321 gccgttatga tgggttgacg ctgaattccg acaccgaaat tctgccggtt ttgggcagta
     1381 gggaggcgtt gttttctttt gtccaaaccg tgttgaaccc tgtttcagac ggcatcaaac
     1441 ccgtaatcgt cagcccgaat cccttttatc agatttatga aggtgcgaca cttttgggcg
     1501 gcggtgaaat ccattttgcc aattgccccg cgccgtcttt caaccccgat tggcgtagta
     1561 tttccgaaga ggtttggaaa cgcaccaaac tggtgttcgt ctgctcgccc aacaacccca
     1621 gcggcagcgt gctggatttg gatggatgga aagaagtttt tgatttacag gataaatatg
     1681 gtttcattat tgcctcggat gaatgctatt ccgaaatcta tttcgacggc aacaaacctt
     1741 taggctgcct gcaggccgct gcacagttgg gtcgaagcag gcaaaaactg cttatgttca
     1801 ccagtttgtc caagcgttcc aacgttccgg gcctgcgttc cggttttgtc gccggcgatg
     1861 ccgaactgct taaaaacttt ctgctttaca gaacctatca cggcagtgca atgagcattc
     1921 ccgtgcagcg cgcaagcatt gccgcttggg atgacgaaca gcacgttatc gacaaccgcc
     1981 gcctgtatca ggaaaaattt gagcgcgtta ttcccatttt gcaacaggta tttgatgtta
     2041 aattaccaga tgcctcgttt tacatttggt tgaaagtccc cgatggcgac gatttggcat
     2101 ttgcacgcaa tttatggcaa aaagccgcta tccaagtatt gcctggacgt tttttggcac
     2161 gggataccga acagggcaat cccggggaag gttatgtgcg tattgctttg gttgccgatg
     2221 tcgcaacttg tgtcaaagct gcggaaacca ttgtttccct atatcggtaa agaataaaaa
     2281 ataccgtctg aacttttgtt cagacggcat ttttcaatat tttacggttg aatttgctat
     2341 aacggtattt acttcacttt taatctaatc ggcttgcccg caaacacgtt taaacttaaa
     2401 atccccgtgt ttgacacaat accgagcaga ttatgttttt tgtcctttcc cctgcgaaaa
     2461 accttaacga aaaagaccct gcccctgtca gcgagtttac ccaacccgac ctgctggcag
     2521 agtccgaaat tctaatgcag cagctgcgcg agcttgcgtt gcaacagatt gccgaactga
     2581 tgcacgtttc cgacaaaatt gccctcttaa acgcgcagcg caatgcagaa tggcacacgc
     2641 cgtttacgcc ggaaaacgcc aaacaggcgg tctttatgtt caacggcgat gtttacgaag
     2701 gtatggatgc aaacacattg gatattggac agatacgcta tctgcaaaac catgtccgcc
     2761 tgctgtccgg tctatacggt cttcttcgcc cgttagacct gatacagccc tatcgtttgg
     2821 aaatggggac ggcatttgcc aatttgcgcg gcaagaattt gtatgagttt tggggcgaca
     2881 tcattaccaa ccttttaaat gacacgcttg cccaagcagg cagcaatacg cttgtcaacc
     2941 ttgcctcaca ggaatatttc aagtccgtca acacgaaaac acttcgggcg cagctgatta
     3001 ccccgatatt taaagacgaa aaaaacggta aatataaaat catcagtttc tatgccaagc
     3061 gtgcgcgcgg attaatggtg cgctatgcgg cagaacatca tattaccgac cctgaaatgc
     3121 tgaaaaattt taattacgaa ggctacgcat tcaatgacgc ggcttcaaat gaaagcgaat
     3181 gggtttttat gcgttcggaa caaataaagt gaaaacaata aattaagtat tttccgaaaa
     3241 aagtgcttgg caaaatgtat aaacttcatt attattcttc ttcttcaaga agacggaagc
     3301 gtggcagagc ggtttaatgc aacggtcttg aaaaccgtcg agggttgata gccctccgtg
     3361 agttcgaatc tcaccgcttc cgccaatcct tggggtcaaa aaccaaataa aaatgaagta
     3421 gcataaagca tcggcatttt tatttggttt tacttcatta tcggaaacgt ggcagagagg
     3481 ctgaatgcag cggactcgaa atccgctgag ggtgcaaatc ctccgtgggt tcgaatccca
     3541 ccgtttccgc cacaaaacaa aaccgccctg attcggggcg gttctttttt gttcaag
//
LOCUS       DAXHTR010000166         3444 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.166, whole genome shotgun sequence.
ACCESSION   DAXHTR010000166 DAXHTR010000000
VERSION     DAXHTR010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.166"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     regulatory      64..167
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            234..1460
                     /gene="cytX"
                     /locus_tag="ACFNML_001858"
     CDS             234..1460
                     /gene="cytX"
                     /locus_tag="ACFNML_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HFC6393175.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARRTGGLKTVSMLLMLLAV
                     LWLSVEVFASSGTNAAPAVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATL
                     TATLAYTLTSCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDA
                     YSAGVSANNISAKLSETPIAVAVAVVGTLLAVLLPVTEYENFLLLIGSVFAPMAAVLI
                     ADFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVL
                     VRLFFKKTQSLQRNPS"
     gene            1457..2557
                     /locus_tag="ACFNML_001859"
     CDS             1457..2557
                     /locus_tag="ACFNML_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFC6393176.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVIKLGRQSIPLWRGIRCRLKTPAMMQENGSLIVWHGQDKPL
                     SNEFVRHLKRGGVADDEIVRWRTDDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECAPEDLQAQYDWLIDCRGYGAKTVWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYSRKRRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            2578..3195
                     /gene="thiE"
                     /locus_tag="ACFNML_001860"
     CDS             2578..3195
                     /gene="thiE"
                     /locus_tag="ACFNML_001860"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFC6393177.1"
                     /translation="MTFPPLKSPLKFYAVVPTADWVGRMVKAGADTVQLRCKALHGDE
                     LKREIARCVAACQGSHTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYIASGAIFPTTTKQMPTAPQGLDKLCEYVKQAGGTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
BASE COUNT          706 a         1083 c          938 g          717 t
ORIGIN      
        1 atgcgccgtg ccgccgaaaa tgtaagaatt ttgccaacgc acgttgcttt gtgtaaactt
       61 aaagctttct tgtcggagtg ccgccgccgg gcggctgaga ttgcgaaagc agaatccgta
      121 gaacctgtcg gggtaatgcc tgcgtaggaa acaaaccgtc aaatgcctta tcgggcttcc
      181 gttccctttt ccgcacttcc ccgcccacct tccttctttt taaaggacgt catatgtcgg
      241 gcaatgcctc ctctccttca tcttccgccg ccatcgggct gatttggttc ggcgcggcgg
      301 tatcgattgc cgaaatcagc acgggtacgc tgcttgcgcc tttgggctgg caacgcggtc
      361 tggcggctct acttttgggt catgccgtcg gcggcgcgct gttttttgcg gcggcgtata
      421 tcggcgcact gaccggacgc agctcgatgg aaagcgtgcg cctgtcgttc ggcaaacgcg
      481 gttcagtgct gttttccgtg gcgaatatgc tgcaactggc cggctggacg gcggtgatga
      541 tttacgccgg cgcaacggtc agctccgctt tgggcaaagt gttgtgggac ggcgaatctt
      601 ttgtctggtg ggcattggca aacggcgcgc tgattgtgct gtggctggtt tttggcgcac
      661 gcagaacggg cgggctgaaa accgtttcga tgctgctgat gctgcttgcc gtgttgtggt
      721 tgagcgtcga agtgttcgct tcgtccggca caaacgcagc ccccgccgtt tcagacggca
      781 tgagcttcgg aacggcagtc gaactgtccg ccgtcatgcc gctttcctgg ctgccgcttg
      841 ccgccgacta cacgcgccac gctcgccgtc cgtttgcggc aaccctgacg gcaacgctcg
      901 cctacacgct gacgagctgc tggatgtatg ccttgggttt ggcagcggct ctgtttaccg
      961 gagaaaccga tgtggcaaaa atcctgctgg gcgcaggttt gggtgcggca ggcattttgg
     1021 cggtcgtcct gtcgaccgtt accaccactt ttctcgatgc ctactccgcc ggcgtaagtg
     1081 ccaacaatat ttccgccaaa ctttcggaaa cacccatcgc cgttgccgtc gccgttgtcg
     1141 gcacactgct tgccgtcctc ctgcccgtta ccgaatatga aaacttcctg ctgcttatcg
     1201 gctcggtatt tgcgccgatg gcggcggttt tgattgccga ctttttcgtc ttgaaacggc
     1261 gtgaggagat tgaaggcttt gactttgccg gactggttct gtggctggcg ggcttcatcc
     1321 tctaccgctt cctgctctcg tccggctggg aaagcagcat cggtctgacc gcccccgtaa
     1381 tgtctgccgt tgccattgcc accgtactgg tgcgcctttt ctttaaaaaa acccaatctt
     1441 tacaaaggaa cccgtcatga cccgtatcgc catcctcggc ggcggcctct cgggaaggct
     1501 gaccgcactg cagcttgcag aacaaggtta tcagattgca cttttcgata aaggctgccg
     1561 ccggggcgaa cacgccgccg cctatgttgc cgccgccatg ctcgcgcctg cggcggaagc
     1621 ggtcgaagcc acgcctgaag tcatcaaact gggcaggcag agcatcccgc tttggcgcgg
     1681 catccgatgc cgtctgaaaa cacctgccat gatgcaggaa aacggcagcc tgattgtgtg
     1741 gcacggacag gacaaacctt tatccaacga gttcgtccgc catctcaaac gcggcggcgt
     1801 agcggatgac gaaatcgtcc gttggcgcac cgacgacatc gccgaacgcg aaccgcaact
     1861 cggcggacgt ttttcagacg gcatctacct gccgaccgaa ggccagctcg acgggcggca
     1921 aatattgtct gcacttgccg acgctttgga cgaactgaac gtcccctgcc attgggaaca
     1981 cgaatgtgcc cccgaagacc tgcaagccca atacgactgg ctgatcgact gccgcggcta
     2041 cggcgcaaaa accgtgtgga accaatcccc cgaacacacc agcaccctgc gcggcatacg
     2101 cggcgaagtg gcgcgggttt acacacccga aatcacgctc aaccgccccg tgcgcctgct
     2161 gcacccgcgt tatccgctct acatcgcccc gaaagaaaac cacgtcttcg tcatcggcgc
     2221 gacccaaatc gaaagcgaaa gccaagcccc cgccagcgtg cgttcagggt tggaactctt
     2281 gtccgcactc tatgccgtcc accccgcctt cggcgaagcc gacatcctcg aaatcgccac
     2341 cggcctgcgc cccacgctca accaccacaa ccccgaaatc cgctacagcc gcaaacgccg
     2401 cctcatcgaa atcaacggcc ttttccgcca cggtttcatg atctcccccg ccgtaaccgc
     2461 cgccgccgtc agattggcag tggcactgtt tgacggaaaa gacgcgcccg aacgtgacga
     2521 agaaagcggt ttggcgtata tccgaagaca agattaaagc cgtctgaaag gaaccccatg
     2581 accttcccgc cactcaaatc cccgctcaaa ttctacgccg tcgtccccac cgccgattgg
     2641 gtggggcgca tggtcaaagc aggtgccgac acggtgcaac tgcgctgcaa ggccctgcac
     2701 ggcgatgaat tgaaacgcga aatcgcccgc tgtgtcgccg cctgtcaggg cagccatacg
     2761 cagcttttca tcaatgacca ctggcgcgag gcaatcgaag caggcgcgta cggcgtgcat
     2821 ctcggacaag aagacatgga caccgccgac cttgccgcca tcgccgccgc cggtttgcgc
     2881 ttgggtttga gtacgcactc cgttgccgaa ctcgaccgcg ccctgtccgt acaccctagc
     2941 tacatcgcca gcggcgcgat tttcccgacc acgaccaagc aaatgcccac cgccccgcaa
     3001 ggcttggaca aactgtgcga atacgtcaaa caggcaggcg gcacgcccgt cgtcgccatt
     3061 ggcggcatcg atttgaacaa cgcccgagcc gtactcgcca ccggcgtttc ctcactcgcc
     3121 gccgtccgcg ccgtaaccga agcggcaaat cccgaagcgg tggttaaagc gtttcaggct
     3181 ttgtgggatg gataaaaccg aaagaagaaa attcaattgc cgtgtaggca aaacttagcc
     3241 cgttatcgca aacatactta actttaaatg tggcatatca tcaaattccg tcattcccgc
     3301 gcaagcggga atccgcctta aaacttgaga aaccatcatt tgaaaaacag tttccgaatt
     3361 tcaaaaatgg attcccgcct gcgcgggaat gacggcaacc ggccggttgc gtatcaaaaa
     3421 ataaagtaat tcggctagat atag
//
LOCUS       DAXHTR010000167         3216 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.167, whole genome shotgun sequence.
ACCESSION   DAXHTR010000167 DAXHTR010000000
VERSION     DAXHTR010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.167"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(1..593)
                     /locus_tag="ACFNML_001861"
                     /pseudo
     CDS             complement(1..593)
                     /locus_tag="ACFNML_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222110.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
     gene            complement(602..1396)
                     /locus_tag="ACFNML_001862"
     CDS             complement(602..1396)
                     /locus_tag="ACFNML_001862"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFC6393178.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIYELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFVHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1780..3114
                     /gene="gdhA"
                     /locus_tag="ACFNML_001863"
     CDS             1780..3114
                     /gene="gdhA"
                     /locus_tag="ACFNML_001863"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFC6393179.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
BASE COUNT          839 a          911 c          770 g          696 t
ORIGIN      
        1 cttgcgcgat gccgttcaag gacaaaccca tacccttttc ggcaaaacgg atttcacggt
       61 cgtcaaacga gactttgccg aagccgaccc gtttcagggc ttccttcacg ctgttttgag
      121 gaggcggcgt ttccgcatcg ggacgggcgg caaaataatc ggcatacagt ttccacaacg
      181 cctgcactgt cggatcgaac gcgccgtccg tcagcgcgtg aatatcgcgg cacaggctca
      241 acagttccaa aaaatccgcc gacggcgaag tcagataacc gtccctgttc aggcggctga
      301 tcaggctgtc ttcacggtaa aggctgaaca attttttcca aacgcgccac ttccgccaaa
      361 accttgttga ccaaatccgc cgcctgtctg tcgtccacac cgaacaggcg cagctccgca
      421 ccggaaccca gcgcgacacc tttccagaag aacacatttt cattgcgttt ttcatcttta
      481 ttgcgttttt catcatcggc ggcaaaagga ttcggcagga aagaaaccgc cgcgcccgcc
      541 gccgcaacgg cggcaactgt caaaaaacgc ctgcgcccga aatgcctgcc cataccgcct
      601 cctaaaccga cactgccgcc ttgatgtgcg gatgggggtc gtaaccttcc aactcgaaat
      661 cttcaaactt gaaggaaaac aaatctttga cttcaggatt gattttcatc actggcaagg
      721 cgcgcggttc gcgttccaac tgcaatgcgg cctgctcgaa atggttgcgg tacaaatgcg
      781 cgtcgccaaa cgtatggaca aactcgcccg cctccaatcc gcacacttgc gccatcatca
      841 tggtcaacaa tgcgtagctg gcaatattaa acggcacacc aaggaaaata tctgcactac
      901 gctggtaaag ctggcaggac agtttgccgt cggcaacgta aaactgaaac agcgcgtggc
      961 agggcggcaa ggccatttcg tcgaccaaag ccggattcca cgccgataca atcaggcggc
     1021 gcgagtcggg gtttttctta atttgttcca ccacattggc gatttggtcg atatgcctgc
     1081 cgtcgggcgc gggccagtta cgccactggt agccgtaaac cgggcctaag tcgccgtttt
     1141 cgtccgccca ctcgtcccaa atggaaacat tgttgtcctt taggtatttg atattggtat
     1201 cgcctttgag aaaccaaagc agctcgtaga taatcgaacg cagatgcagc tttttggtcg
     1261 tcagcagcgg aaaacctttg cccaagtcaa aacgcatctg ataaccgaat acggagcgcg
     1321 tgcccgtacc ggtgcggtct gatttgtccg taccgttgtc gaggacgtgg cgcatcaagt
     1381 ccaaataggc tttcatagca gtctttcatc aaattaaacg gcgcatattg taacatttcc
     1441 ggataatgcc caaaacacgg atacagacag gcaggattgt tggcaatttc agtccttttc
     1501 cacagtaaaa cccggtggga aaacaaaatt accttgattg gaatcaaaaa atctagttta
     1561 attacttaga ataaaatttc aataatattt ttatttacga aattaattta tgattattga
     1621 ttaattatcg gcaacaaaca tcaaacatcg aaaacatgga aaaaataatg tcaacaattt
     1681 ttgccaaatc gggcttggca tcagaaaaaa ataggtttat attcccacct acaaatttgt
     1741 tttcccatta gtacactatc aaccaaaagg agtatccgaa tgactgacct gaacaccctg
     1801 tttgccaacc tcaaacaacg caatcccaat caggagccgt tccatcaggc ggttgaagaa
     1861 gtcttcatga gtctcgatcc gtttttggca aaaaatccga aatacaccca gcaaagcctg
     1921 ctggaacgca tcgtcgaacc cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat
     1981 aaagggcaag tccaagtcaa ccggggctac cgcgtgcaaa tgagttccgc catcggtcct
     2041 tacaaaggcg gcctgcgctt ccatccgacc gtcgatttgg gcgtattgaa attcctcgct
     2101 tttgaacaag tgttcaaaaa cgccttgacc accctgccta tgggcggcgg caaaggcggt
     2161 tccgacttcg accccaaagg caaatccgat gccgaagtaa tgcgcttctg ccaagccttt
     2221 atgaccgaac tctaccgcca catcggcgcg gacaccgacg ttccggccgg cgacatcggc
     2281 gtaggcggac gcgaaatcgg ctatctgttc ggacaataca aaaaaatccg caacgagttt
     2341 tcttccgtcc tgaccggcaa aggtttggaa tggggcggca gcctcatccg tcccgaagcg
     2401 accggctacg gctgcgtcta tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt
     2461 gaaggcaaac gcgtcctgat ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa
     2521 gccatccaac tgggcgcgaa agtgctgacc gtttccgact ccaacggctt cgtcctcttc
     2581 cccgacagcg gtatgaccga agcgcaactc gccgccttga tcgaattgaa agaagtccgc
     2641 cgcgaacgcg ttgccaccta cgccaaagag caaggtctgc aatactttga aaaccaaaaa
     2701 ccgtggggcg tcgccgccga aatcgccctg ccctgcgcga cccagaacga attggacgaa
     2761 gaagccgcca aaaccctgtt ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg
     2821 ccgtcgactt tgggcgcggt cgagcaattt atcaaagccg gcatcctcta cgccccggga
     2881 aaagcctcca atgccggcgg cgtggcaact tcaggtttgg aaatgagcca aaacgccatc
     2941 cgcctgtctt ggactcgtga agaagtcgac caacgcctgt tcggcatcat gcaaagcatc
     3001 cacgaatcct gtctgaaata cggcaaagtc ggcgacacag taaactacgt caatggtgcg
     3061 aacattgccg gtttcgtcaa agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac
     3121 gccgccgctc cgcaaacaaa atgccgtccg aaccgcaaat gctgttcaga cggcatttca
     3181 ttatccgccc gttcgaatcg ggtgatactt atagtg
//
LOCUS       DAXHTR010000168         3198 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.168, whole genome shotgun sequence.
ACCESSION   DAXHTR010000168 DAXHTR010000000
VERSION     DAXHTR010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.168"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..302)
                     /locus_tag="ACFNML_001864"
     CDS             complement(<1..302)
                     /locus_tag="ACFNML_001864"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393180.1"
                     /translation="MIASNAKNRAQNPSAAYRPALFGQTSILPPSRTLVQTGTGDTAR
                     AFPVFNGYPSSGLCSSTISASRFSGGSRRKYSRLNLNQYSVASPCRTDLNLIHYIL"
     gene            358..981
                     /locus_tag="ACFNML_001865"
     CDS             358..981
                     /locus_tag="ACFNML_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFC6393181.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIMLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1092..2474
                     /locus_tag="ACFNML_001866"
     CDS             1092..2474
                     /locus_tag="ACFNML_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6393182.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFVLGIVCIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGAAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQGNQDGLQCALSQLEGVEDIGFSFDRQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2478..3002
                     /locus_tag="ACFNML_001867"
     CDS             2478..3002
                     /locus_tag="ACFNML_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFC6393183.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          656 a          862 c          952 g          728 t
ORIGIN      
        1 ctcaagatat agtggattaa atttaaatca gtacggcaag gcgaggcaac gctgtactgg
       61 tttaaattta atctactata tttccgcctg ctgccgcccg aaaagcgtga tgcgctgatg
      121 gtgctgctgc acaatccgga cgacggatag ccgttgaaaa cgggaaatgc ccgggcggtg
      181 tcgcctgtgc ctgtttggac aagcgttctt gagggcggta gaattgaggt ttgcccgaaa
      241 agggcagggc gatatgccgc gcttggattt tgcgcgcggt ttttcgcgtt ggaagcaatc
      301 attgtctgtt tgaatattta atgccgtctg aaacgtccgg acgcgccccc gttccctatg
      361 aaaaaaccga ccgataccct acccgttaat ctgcaacgcc gccgcctgtt gtgtgccgcc
      421 ggtgcgttgt tgctcagtcc tctggcgcac gccggcgcgc aacgtgagga aacgcttgcc
      481 gacgatgtgg cttccgtgat gaggagttct gtcggcagcg tcaatccgcc gaggctggtg
      541 tttgacaatc cgaaagaggg cgagcgttgg ttgtctgcca tgtcggcacg tttggcaagg
      601 ttcgtccccg aggaggagga gcggcgcagg ctgctggtca atatccagta cgaaagcagc
      661 cgggccggtt tggatacgca gattatgttg gggctgattg aggtggaaag cgcgttccgc
      721 cagtatgcaa tcagcggtgt cggcgcgcgc ggcctgatgc aggttatgcc gttttggaaa
      781 aactacatcg gcaaaccggc gcacaacctg ttcgacatcc gcaccaacct gcgttacggc
      841 tgtaccatcc tgcgccatta ccggaatctt gaaaaaggca acatcgtccg cgcgcttgcc
      901 cgctttaacg gcagcttggg cagcaataaa tatccgaacg ccgttttggg cgcgtggcgc
      961 aaccgctggc agtggcgttg attttgaacc cgcgccgcaa ccgaaatacg gcgaatcctg
     1021 tataatccga aaatctgttc actggaagtt cagacggcat tgcaactgtt gatgccgtct
     1081 gaaaaaatat gatggcaaga gataaccgca tccaaatgtt tccgcacgaa tggcgcgcca
     1141 gtacgacgct ttccggcgtg tacgcgctgc gtatgctggg tatgttcctc gtgctgcccg
     1201 tattggcggt gtatgccgcc tcgctgcccg gcgcggaagg caacaaaacg ctggtcgggc
     1261 tggcaatggg catttacggg ctgacacagg ctctgctgca actgcctttg ggcatcgctt
     1321 ccgacaagtt cgggcgcaag aaaaccattt atgtcgggct gattgtgttt gcagcgggca
     1381 gcttccttgc cgccgccgcc gatacgctgc ccatgctggt cgccgcacgc gccatacagg
     1441 gtgcgggagc ggtcagtgcg gcggttaccg cgctgctggc ggatttgacg cgcgacggcg
     1501 tacgtacccg cgcgatggcg atgatcggtt tgagtatcgg tctgacgttt tcggtcagcc
     1561 tcgtcgttgc ccctgtgatt gccgacgcgg tgggcgtacg cggcttgttt ctgctgaccg
     1621 gtattttgac cgttgccagt atcggcgtgg tggcgtggat gactcccgac ccggaggttt
     1681 ccaagctgca cgaagatacg caggcgcagc cttcgcgcat aggcgaagtt ttgaaaaacc
     1741 gtaggctgct gacgctcgat ttcggcattt tcgccctgca cgccgcgcaa atggcattgt
     1801 ttaccgcgct gcctttcgcg atgacccagc tcggtttgga aaaaatccag cactggaaag
     1861 tctatctgcc ctcgaccatt acgggattgg tggtaatggt tccgctgatt atcgtcggcg
     1921 agacgcgcaa caagcttaag caggtttttg ttttgggtat cgtctgtatt gcggcggcgc
     1981 agctcggttt gctgtccggt atgcgctcgg taggcttgat taccgcttat ttggttgttt
     2041 actttatcgg ttttaatgtg ttggaagcga gcctgccgtc tatggtttcc aaaatcgcgc
     2101 cgtccgacct gaagggtacg gcgatgggcg tgtacaacac gatgcagtcg ctcggactgt
     2161 ttgccggcgg cgcggcaggc ggtttgctgt ttcaaaaata cggcttttcc ggcgtgtttg
     2221 ccttttgcag tatattgatg ctgctgtggc tggtaattgc cgttttatcg cccgcgccca
     2281 agcccgtcaa aaacctcagt taccctgtcg gcggcgtgtg gcagggcaat caggacggct
     2341 tacaatgcgc cttgtcgcaa ctcgaaggcg tggaagacat cggtttcagt ttcgacaggc
     2401 agaccgtcta tctcaaagtg ttgcagaagg gtttcgatca ggctgccgct gaaaaaatca
     2461 tcacaggagt ttaaaaaatg tcattgaaca aagtcatcct tatcggccgt ctcggacgag
     2521 atcctgaagt acgctatatg cccaacggcg aagccgtctg caatttcagc gtcgccacca
     2581 gcgaaacttg gaacgaccgc aacggtcagc gcgtagagcg caccgagtgg cacaacatca
     2641 cgatgtaccg caaactggcg gaaattgccg ggcaatacct caaaaaaggc gggctggttt
     2701 atctggaagg cagaatccaa agccgcaaat accaaggcaa agacggcatc gaacgcactg
     2761 cttacgatat tgtcgccaac gaaatgaaaa tgttgggcgg gcgcaatgaa aacagcggcg
     2821 gtgcgcctta cgaggaaggt tacggtcaga gtcaggaggc ttaccaacgc cccgcgcagc
     2881 aaagccggca gcccgcctcc gacgcgccgt cccatcccca agaagcacca gccgcgccgc
     2941 gccgccaacc cgtgcctgcc gccgccccgg tcgaggacat tgacgacgac atcccgttct
     3001 gaattttacg gcagcacatc ctcgcggagg gaaatcataa aggacggaga aaccttaaac
     3061 cttacggggc agggtttctc ttttttgcat ttgtgccggc ttgttgctgc cccctgcgtt
     3121 gggcggaacg ttgcgattgc gccgttctgg cgttttgagg cagtgatgcc tgccgcaggc
     3181 ccacgctgtt ttcagacg
//
LOCUS       DAXHTR010000169         3167 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.169, whole genome shotgun sequence.
ACCESSION   DAXHTR010000169 DAXHTR010000000
VERSION     DAXHTR010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.169"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(27..260)
                     /locus_tag="ACFNML_001868"
     CDS             complement(27..260)
                     /locus_tag="ACFNML_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393184.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPRRRESRPFGFGFFG"
     gene            complement(290..3058)
                     /gene="tdfH"
                     /locus_tag="ACFNML_001869"
     CDS             complement(290..3058)
                     /gene="tdfH"
                     /locus_tag="ACFNML_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent zinc piracy receptor TdfH"
                     /protein_id="HFC6393185.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRTGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQQYFVQTGGLKFNSNSGKWER
                     DLQRQFWKTSYYKQYKDPQKLKKYIEDQDKDWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNPYTNLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSEGEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGDIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEEVTCNDDKTLCNGKYGGTSKSVLTNFARGRTF
                     LMTMSYKF"
BASE COUNT          727 a          768 c          786 g          886 t
ORIGIN      
        1 gaatctagaa atttaatgtt gcggcactaa ccaaaaaaac cgaaaccgaa cggtctagat
       61 tcccgcctgc gcgggaatga cgactcataa attacccgaa acaaccaaaa ataaccgaaa
      121 tcaaaaaccg gcttccctgc gtgggaatga aatggatttt tctttgagtt gccgatgcga
      181 taaaacggat tggcataggc ttgcggcagg atgatgcgtt tggggaaaca aatgccgtct
      241 gaagggcttt cagacggcat tgcggttttc tacaaaacgc gggctgcctt taaaacttgt
      301 agctcatcgt catcaaaaag gtgcgtccgc gtgcaaaatt ggtcaatacg cttttgcttg
      361 tgccgccgta tttgccgttg cacaacgttt tatcatcatt acacgttact tcttcgtcct
      421 tgtctttcgg gtcgaacgag ctgtaataac gctgcgttgc cgcatcattg cccgcatcga
      481 gcggatcgat ataacgcctg tcgaacagat ttttgacttc ggcgcggaaa ataaggtttt
      541 tcttcggctc gtaagcggcg taaaaatcaa aaatcaaagg ctggcgggca agggtttcgg
      601 tttgtttgat ggaacgcttg cccagttgcc ggacattgct ggtatttccc ccgttggtgc
      661 cgtcgatata gcgttcttca gccgtcgcgc ggatgctctt gccgaaatag cgcatcgcgc
      721 cgcccaaagt cagtttgttg cccaaccagc gcgtaccgac ttccaaacgt ccgtaatctc
      781 gcggcagggc ggaaaccctg ctcaacccat aaccttgttt gagttggtct tctttggacg
      841 cattgttggg cgattcgctc gcatcgctga agttggtcgg ttgcgtgctt ttttgatagg
      901 cgtaagaaag gttggtgaaa aaacgcccat aatcgtaatt cagctccaac tcaaaaccgt
      961 gtttgtgcac tttgtctttg aaattgcgat gttggatggt gtaggcaagc ccggtgctgc
     1021 tgacccagct cggaatatcc ccgttcaaat cccaccattt cccgtaaacg ttgtggatgt
     1081 agttgtcgat gcggctgcgg tagccgacca gttttaatcc taatgtatca tcttgtttta
     1141 acagtccttt tttataggta ttgaagccaa attgccaagt gtttgcgcgc tctggtttta
     1201 aggcggtgtg aacgccggag tcgccgattt gggaaaaata catttcttgg atgttgggca
     1261 tacggtgtgt gcgcgaatag ctggcgaacg gcatgaaata atcgccgaag tccgcactaa
     1321 tgctgaccga atggttgttg gcgcgctttt tgccgtattt tttcaatacg ggttcataaa
     1381 gcccgcagct cgggttgcaa tgttccctgt atgtcggcga gttttctccg aatgcccgct
     1441 taaattcgcc ttccgagctg taatagcccg tatattcgcc gccgaaacgg tagccgacgg
     1501 tattggtgct gtagtttaag cggtaaatgt cttttttgag cgcggcatcg aagtagaacg
     1561 tgttgaaata ttggctgccg gccggttgga caatggttga tttttggggc agcagccctt
     1621 tatcgccctt aaaccgcccc aaataggaat aaagcccgtt gtcctgatca ggaccgtcga
     1681 aaaacagccc caattcttca ggaaagcggt ttttgccgta ttcgttgtgg aaataattga
     1741 agcccaaagt ggtttgcaac tcggtttcgc ggggcagccg gaaggtggcg gtgttgttga
     1801 ggtcgaggat tttcgcgttg ttgtaggttt caaaatcctt taaaagcccc cagcctgtaa
     1861 acttcgaccc tttcggatat ttctgcctgc ccgaattgta ggctgcggtc agattgaggt
     1921 tggtatacgg gttcaaagac aaaccgtaat tgaactgata attgcggttg atgattttgc
     1981 ggctgccgat tttggtgttt aaatcgcgaa attgcgccgt gtatttattg aatacgccgt
     2041 cgtattccaa tttaaacaga ttgcctgccg actgctgctt caggctggac ggatcgatgg
     2101 gggtgatgtc gtattgcggc gccaggtttt cccgccagtc tttatcttgg tcttcgatgt
     2161 attttttcag tttctggggg tctttgtatt gtttatagta actggttttc cagaattgcc
     2221 tttgcaaatc gcgttcccat tttccgctgt tggaattgaa tttcaacccg cctgtttgta
     2281 caaaatattg ctgcttgcgc cgttccagat attccgcgcc aaaatttccg atgtgctgcc
     2341 cgccgccgcc cacgcggtaa ttttgcgcca cgctgcgcct gctgtgcccg taaagcacac
     2401 cgacagatgc tccgctttcc agccatttgc gcgcacctat cgccgccatc gcattacctt
     2461 tggttgaatt ggtgccggtc agacctttta gcagcaggcc gtaggtatta ttgccctgaa
     2521 cgacgtcatc cacgcctaaa gtccgcagat tcgccgaacc ggcaaggctg ttgatgcctg
     2581 ccgagccgct gaagctgcct ttgacgacat ccagtccggc aataaaattg ctgtcgacag
     2641 atgcaccgaa ttgagatgaa ccgcctgccc tgcccgcatc ggtagaagtc gaataaaagg
     2701 tctgcgtgat accgtccacc atcgtattga cccgcccgaa cccgctgtcg ccgcgaatat
     2761 tcaaagacac aatgcccgag cttttatctt gctgtgtaaa cgcaccgggt atgctgcgta
     2821 cgatgttgtc gaggttttcg ctggatttga atatatcctg acgggtcgat acggcacgcg
     2881 catcggtaaa cacttttttg tctttcggta cgcgcttcgc cttgacgtgc acatcttcca
     2941 aaacctgtat ctgcgcctcg ctgcccgtgc gccctgcatc ttcggcataa ctatgatgat
     3001 atagcataac acccataaga taaaaacaaa tcggcttcaa ccggaaagaa gatctcatat
     3061 tttcctcaac aataaacagt cagacaatta agaaatatat tagcattttt ttaggcggca
     3121 taaacattaa aaagtgtaaa ttcgatatac cgtctgaaga tttcaat
//
LOCUS       DAXHTR010000170         3165 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.170, whole genome shotgun sequence.
ACCESSION   DAXHTR010000170 DAXHTR010000000
VERSION     DAXHTR010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.170"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(75..668)
                     /gene="ribA"
                     /locus_tag="ACFNML_001870"
     CDS             complement(75..668)
                     /gene="ribA"
                     /locus_tag="ACFNML_001870"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFC6393186.1"
                     /translation="MSELLNYVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(661..1335)
                     /locus_tag="ACFNML_001871"
     CDS             complement(661..1335)
                     /locus_tag="ACFNML_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393187.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAAYLTAQTVMGMGTASIDRSV
                     LWRSDEGWKLADYLSCDNVREDALKRLFMALDSVFSRSTGVRSAAVYALMPSENQAFQ
                     LICLSQQGEGLENIWNLDENVADVSLACRCAQSGWMNVASDVRRWLDLGELSGERNHA
                     SAAQISIPICTESGGVLGVVHVEFECAECADEAAQAEWVALALALSEPLKQLLGITAA
                     EGDENV"
     gene            1489..1573
                     /locus_tag="ACFNML_001872"
     tRNA            1489..1573
                     /locus_tag="ACFNML_001872"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1523..1525,aa:Leu,seq:tag)
     gene            2093..3127
                     /gene="purM"
                     /locus_tag="ACFNML_001873"
     CDS             2093..3127
                     /gene="purM"
                     /locus_tag="ACFNML_001873"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFC6393188.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
BASE COUNT          807 a          834 c          784 g          740 t
ORIGIN      
        1 ttattgcttt atcaaggtat ggaaacctgt ttcccgaaag gcggcgcagg atgcccgttc
       61 cctgcaactt tgccttattc cgacatcaga tgtcccagct tgtctgcttt ggtttggagg
      121 tagcgtttgt tttcaaggtt ttccccgacg tgcaggggga tgcgttcgac cacgttaatc
      181 cccgcatctt tcagggtttg gattttttcg gggttgttgg tcagcagttt gaccgagcgg
      241 atgcccagat attcgtagat agattgcgcc aaacggaaat cgcgggcatc gacgggcagc
      301 ccgagtgcca aattggcttc aaccgtatcc ataccttggt cttgcagatg ataggcgcgg
      361 attttgttaa tcagcccgat gccgcgtcct tcctgacgca gatagacgat gatgccgcgc
      421 ccctctgtct gtaccgccct catggccgct tcaagttgag gtccgcagtc gcattttctc
      481 gagaacagcg catcgcccgt caggcattcg gagtggatgc gcgtcagaac cggattgccg
      541 tctgaaaaat caccgacggt cagcgcgacg tgttcctgcc cgtttgcctc ttcaaagccg
      601 tgcatcgtaa atacgcccca ttcggtcggc aggcggcagg aagcaacata gtttaataat
      661 tcagacattt tcatctcctt ctgcggcagt gatgcccaaa agctgcttca aaggttcgga
      721 caaagccaag gcaagagcca cccattccgc ctgcgctgcc tcatccgcac actctgcgca
      781 ttcaaattcc acatgaacca cgcccaacac accgccactt tccgtgcaga ttggaatgga
      841 aatttgcgcc gccgaagcat gattgcgttc tcccgaaagc tcccccaaat ccaaccaacg
      901 gcgtacatcc gaggcaacat tcatccagcc gctttgcgcg caacggcagg caagtgaaac
      961 atcggcaaca ttttcatcta gattccatat gttttccaaa ccttctccct gttgggacag
     1021 gcatatcagt tggaaagcct ggttttcaga cggcatcaag gcatagaccg ccgcactccg
     1081 cacgcctgtc gagcgcgaaa acaccgaatc caaagccatg aaaagccgtt tcagtgcatc
     1141 ttcgcggaca ttgtcgcacg acaggtaatc ggcaagtttc caaccctcat cactgcgcca
     1201 caaaaccgaa cggtctatcg aagccgtccc catacccatt accgtctgcg ccgtcagata
     1261 cgccgcccga acctcgtcaa gcggcagctt caaaccctga gtcagcagaa aatccttaat
     1321 caaagcagca ggcataccca aattccgtca ataaaaacaa aaaaccgccc gattccgatg
     1381 tcaggcggac gaaaaacaga ttttaccgcc ccaaacccca agccatcaac agccccgccg
     1441 aaaaccttgc acaaaaaaac aaaatctgta taatcgtcca attcccatgc ggacgtggcg
     1501 aaattggtag acgcaccaga tttaggttct ggcgccgaga ggtgtgagag ttcgagtctc
     1561 tccgtccgca ccaataatga taaataaatt atatagttaa tgcttatcgg gacaaattag
     1621 tgccacaaaa cataagtcgt ctgcttttca gcgggcggct tttttatctg cctatatgtt
     1681 tcaacacgca gaacggtaca taaagtgccg ccatttgttc cgcaccgaat cggaaacggg
     1741 tgcttccgcc cgcaatcaga ttcaccgccc ctaatgccgg ggtttcaacc ggaaagaaga
     1801 aaatattgat tttgatgatg gcatcaaaaa accccttccg ctcgtctgcc aaaaggaatg
     1861 gttaagaaaa gcaatgcgtc ttttgatgga acgcgatata taaggaattg aggggaaggg
     1921 caggcgggtt tctgcccgtt aatcctgcgg acggtcccgc caaacccgac acgcgcgggt
     1981 atatggcgga ctgtttacaa tattgtctaa aaatctgaac ctgcaaacgt gaaataaggt
     2041 agaatacgcc ccgttatttt accgtccaat ccccatttac caaggaaaaa cgatgagtac
     2101 ttcattgagt taccgcgatg caggtgtcga tatcgacgca ggcgaccaac tggtcgaaaa
     2161 catcaaaccg tttgccaaac gcaccatgcg tccggaagta ttgggggatt tgggcggttt
     2221 tggcgcattg gtcgaaatcg gcaagaaata taaaaatccc gtattggtca gcggtacgga
     2281 cggcgtgggt accaagctca aacttgcctt cgattgggat aaacacgata cggtgggcat
     2341 cgaccttgtt gcaatgagtg tcaacgatat tttggttcaa ggagcagaac ctctgttttt
     2401 cttggattat ttcgcctgcg gcaaattgga tgttccgcgc gcaaccgatg tcatcaaagg
     2461 cattgcacaa ggttgcgaag aatccggctg cgccctgatt ggcggggaaa ctgccgaaat
     2521 gccgggtatg tatcccgtcg gagaatacga tttggcgggt tttgccgtcg gcgtagtgga
     2581 aaaagagaat gtcattaccg gccgcagcat cggcgcgggc gatgtggtat tgggtttggc
     2641 ttccaacggc gcacattcaa acggctattc ccttatccgt aaaatcatcg aacgcgacaa
     2701 tcccgatttg gatgccgagt ttgataatgg caaaaccttg cgtgaggctg ttattgcgcc
     2761 gacccgtctg tatgtgaaac cgattcttgc cgctttggaa aaatttacca ttaaaggtat
     2821 ggcacacatt accggcggcg gcattaccga aaacgtgccg cgcgtgttgc ctgaaaacac
     2881 ggttgcacaa atcgatgcta aatcgtggga attgcccaag ctcttccaat ggcttcaaaa
     2941 ggcgggcaat gtggaaaccc aagaaatgta ccgaaccttt aactgcggca tcggcatggt
     3001 cgttattgtt gctgccgaag atgccgatgc ggttcagggt ctcttgggtg aacaaggtga
     3061 aacagtttac cgtttaggtt tgatccgtga gcgtcaggga gacgagcatc aaacccaggt
     3121 tgcctgattg cttctatagc gaaatgccgt ctcaaacatg ggttt
//
LOCUS       DAXHTR010000171         3140 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.171, whole genome shotgun sequence.
ACCESSION   DAXHTR010000171 DAXHTR010000000
VERSION     DAXHTR010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.171"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..380)
                     /locus_tag="ACFNML_001874"
     CDS             complement(<1..380)
                     /locus_tag="ACFNML_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393189.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="ACFNML_001875"
     CDS             complement(438..803)
                     /locus_tag="ACFNML_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393190.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="ACFNML_001876"
     CDS             complement(785..1330)
                     /locus_tag="ACFNML_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393191.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
     gene            complement(1493..1765)
                     /locus_tag="ACFNML_001877"
     CDS             complement(1493..1765)
                     /locus_tag="ACFNML_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393192.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYIIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
     gene            complement(2641..2889)
                     /locus_tag="ACFNML_001878"
     CDS             complement(2641..2889)
                     /locus_tag="ACFNML_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393193.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
BASE COUNT         1013 a          681 c          452 g          994 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttggaaa atcaaagccc tgcttagaac
     1381 gtatgttgca cacccaattt caataggtaa atcagattgc aaatccagca atttgaatat
     1441 tgtcattgtt ccgtgcaaaa gggattttta ttgatgagtt gtgtactggg tttcagcttg
     1501 gctttttaga taatcttttc acaagaacat acatcaatac tgtccaaaaa aaaagatttg
     1561 ataaatatat tgcaacatat tttattccac ctccatccga aacagaaacc aatatatttt
     1621 taattaatac atagctgatt attacattta ttattcctat aatatataag gatcttgcca
     1681 aaattttttt tgattttatt ttaggaatat ctcttatcca tgctaaaata cagcccaatg
     1741 tcgaaaagaa aataacagca aacactacct tacctcctct cacttccaac caaaccgata
     1801 gcaggtttgg ttggaagttg gtttatttat tatttaacgg cgaatgtcag tgttcttacc
     1861 cgtagaacct gcataaccat ttatgccacc agcaacagtc cccactgcta cacctcgcat
     1921 accattaatc agaattcttc ctattttttc gaatccacta tttcttccaa agatgcagca
     1981 aacgcaggtt aagcctagac ccaatctacg tttgctgccg catctgatcc ctattgtttc
     2041 ttatccttac atcttcctgc cttgtcaatc aaataaagac agaagactat acaaaaactg
     2101 accgacatac tgaaaatacc tatccccttc catgcaatct ccgcaccatt gacccaatag
     2161 atcagactga gaaacaacaa agccacagta taaatcaacg taaaaaatat tgcgtaaagc
     2221 acaataaagg gaacttttat ctcacggttg ttttttataa tacattcagc aacttgattt
     2281 ccgaatatac ctgataaaaa atatagtatt agatcatcca tttcgtttat cttctatgtt
     2341 ttcccattgc ggcgctagaa gactgattta atgctgcacc atgcaatata gtgaattaac
     2401 aaaaaccagt acggcgttgc ttcgccttag ctcaaagaga acgattctct aaggtgttga
     2461 agcaccgagt gaatcggttc cgtactatct gtactgtctg cggcttcgtc gccttgtcct
     2521 gatttttgtt aatctactag agtttaaaca actttatttt tgattttatc ccaagtataa
     2581 acctgacaat cgtatttaaa agccggatat aagcctagct tgttcactgg tatgtatgat
     2641 ttagactaaa aagaaatcaa tcatttgctc aattttaagc gtctccccga caggtttccc
     2701 ggtctcagga tcaaactcaa gaaaacctga taaaaaatca ttaataatca ataatttttc
     2761 atcactggaa acagtatttt ttataatttt ttcccttagt acggataaag caaaattttc
     2821 taaagaacta tattgccccc catcaatttt tttagaaatt aaagcaacta tttcattttt
     2881 aatattcata aattaccctt aaatgataaa gggagcgaag cccgcttaaa acgtataact
     2941 gtatacccaa tttcagcaag cacaggttga gttgccaagc ctaacagagc ctgaatgatt
     3001 ggttataact caaccttata tctacactta ccgaaaacct caaataactt aataaaaatc
     3061 acacctaata cttactgaaa cgataaacta cattttcttt aaataaggtt ttatctgtta
     3121 tctcatttaa agacaataaa
//
LOCUS       DAXHTR010000172         3118 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.172, whole genome shotgun sequence.
ACCESSION   DAXHTR010000172 DAXHTR010000000
VERSION     DAXHTR010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.172"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(125..976)
                     /locus_tag="ACFNML_001879"
     CDS             complement(125..976)
                     /locus_tag="ACFNML_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HFC6393194.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDAEFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QESACVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALKKFDPAGVAA
                     ADLNESLILQIERSGECAAKPSALHIVRNALDSIDGNRSQTLARIKKRLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESFEIPAA
                     HKRKTAE"
     gene            1324..2838
                     /gene="katA"
                     /locus_tag="ACFNML_001880"
     CDS             1324..2838
                     /gene="katA"
                     /locus_tag="ACFNML_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HFC6393195.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGIGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2914..>3118
                     /locus_tag="ACFNML_001881"
     CDS             2914..>3118
                     /locus_tag="ACFNML_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393196.1"
                     /translation="MPSETSFDRFRRHFPIPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFPRHSRESGN"
BASE COUNT          746 a          882 c          810 g          680 t
ORIGIN      
        1 gtttctagat tccctctttc gcgggaatga cgggatgcag gtttccgcgc ggacggattc
       61 ggcattcccg ctttcgcaga aatgccggct tttggtttgt tgtctttata agattattcg
      121 gcaattattc tgcggttttg cgtttgtgtg ctgccggaat ctcaaaggat tctctgtatt
      181 tggcaatggt gcggcgagaa acctccatgc cgcggaaagc cagcaggttg gcgagcgcct
      241 cgtcagaata cggcgtgggt gtggacgagg caaaaccggc ggcgggaaag ggattgagcg
      301 aagcaatgag gtcgagtgcg gcttcgagtg tgccgctgtc ggtttggggc aggcgttttt
      361 ttattcgcgc gagggtttgg ctgcggttgc cgtcaatgct gtcgagggcg tttcggacga
      421 tatgcagggc ggagggtttg gcagcacatt cgcccgatct ttctatctgc agtatcagcg
      481 attcgttcaa atcggcggcg gccacgcctg ccgggtcgaa ttttttcaat gcggtcaggg
      541 cttgtttcag cattgcttca tccaacatcc actccaaggg cgtatggtcg aggatgtctt
      601 cgatgctgtc ggtcagataa ccttgctcgt caaggaaatc gatgaggatg tggacacagg
      661 cggattcttg gtcggaaagc gggtgttcgc atacttgcgc gtgcaggtat tgcttgaaat
      721 cctgctcgcc ggcgatgttg gacagcatat cttcgccttc gtctccgccg atttgacggg
      781 caggcgcagt gtaatggctg aactcggcat cggaaaattc atccgtgtct ttgcgttcga
      841 gcagggggtt gtccgacagc cagttttcga cctcgcgttc aagttcgata cccgacatct
      901 gcaatacgcg caaagattgt tgcagccgct ggttgagctg ctgggtctgt ttgagcttta
      961 ttccgagtaa ggtcatgata atgtggggaa aattgttatt ttcagcctgt cggcgcaaaa
     1021 aatgccgcaa agcgtcattg cattataaat ggttttaatg agcgggttcg gattccgttc
     1081 gataacaaaa aacaaacgaa aatcaagaac cgattgctta taataatatt aaatcgattt
     1141 catagtttta atagcgaaaa tcttggcgta tagtcgcatc catagttttt acaaaaggga
     1201 aataaaatgt cgattcaaga aatttattgc aatcaagaaa ccggttacga atacgctttc
     1261 cgccaaatcg tactgtaagc cgtttacccc catttgacca acctgagaaa aggaacaaga
     1321 gcgatgacta cctccaaatg ccctgtaacc catctgacca tgaacaacgg cgcgcctgtt
     1381 gccgacaatc aaaacagcct gaccgccggt cctcgcggcc ctctgctggc gcaggatttg
     1441 tggctgaatg aaaaactcgc cgacttcgtg cgcgaagtca tccccgaacg ccgtatgcac
     1501 gccaaaggtt cgggcgcgtt cggtacgttt accgtaacgc acgacatcac caaatacacc
     1561 cgcgccaaaa tcttcagcga agtcggcaaa aaaaccgaga tgttcgcccg tttcaccacc
     1621 gtggcaggcg aacgcggcgc agccgatgca gaacgcgaca tccgcggttt tgcgttgaag
     1681 ttctataccg aagaaggcaa ctgggatgtg gtcggcaaca acacgcccgt gttcttcctg
     1741 cgcgacccgc gtaagttccc cgacctgaac aaagccgtca aacgcgaccc gcgcaccaat
     1801 atgcgctctg ccacaaacaa ctgggacttc tggacgctgc tgcccgaagc actgcaccaa
     1861 gttaccatcg tgatgagcga ccgcggcatc cccgccggct accgccatat gcacggcttc
     1921 ggttcgcata cctacagctt ctggaacgaa gcaggcgagc gtttttgggt gaaattccat
     1981 ttccgcaccc aacaaggcat taaaaacctg accaacgaag aagccgccaa aatcatcgcc
     2041 gacgaccgcg aaagccatca gcgcgactta tacgaagcca tcgaacgcgg cgagtttccg
     2101 aaatggacga tgtacatcca agtcatgcct gaagcagacg cggaaaaagt accttatcat
     2161 ccgtttgact tgaccaaagt ttggccgaaa aaagactatc cgctgattga agtgggcgaa
     2221 ttcgagttga accgcaatcc cgaaaacttc ttcgccgatg tggaacaatc cgccttcgca
     2281 ccgagcaacc tcgttcccgg tatcggcgcg agcccagata aaatgttgca agcccgtttg
     2341 ttcaattacg ccgacgcgca acgctaccgc ttgggcgtga acttccgcca aatccctgtc
     2401 aaccgtccgc gttgccctgt tcacagcaac cagcgcgacg gacaaggacg cgccgacggc
     2461 aactacggca gcctgccgca ctacgaaccc aatagcttcg gccaatggca gcaacaaccc
     2521 gacttcgccg aaccgccttt gaaaatcaac ggcgacgcgg cgcactggga ctaccgccaa
     2581 gacgatgacg actatttcag ccaaccgcgc gccctgttca acctgatgaa cgacgcgcag
     2641 aaacaggcat tgttcggcaa caccgccgcc gcaatgggcg acgcgcccga cttcatcaaa
     2701 taccgccata tccgcaactg ctaccgttgc gatccggcat acggcgaagg cgtggcaaaa
     2761 gccctcggac tgactgtcga agacgcacaa gccgcccgcg cgaccgaccc cgcactcggc
     2821 cagcccggtt tgctgtaagg gggcattatg tggatggaaa ttgaagaaat cctgtcccgc
     2881 gccgtccgct accggaaata accgggcata aaaatgccgt ctgaaacgtc gtttgaccgc
     2941 ttcagacggc atttccccat cccgcccccg ccgtttcagt gggcgttttt tattaaacgc
     3001 aaaatatccc gtcattcccg ccccacagcc cacttttccg ccgtcattcc cgcaccatcc
     3061 ccaccggttc aaaccggcac gaaaactttt ccgcgtcatt cccgcgaaag cgggaatc
//
LOCUS       DAXHTR010000173         3078 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.173, whole genome shotgun sequence.
ACCESSION   DAXHTR010000173 DAXHTR010000000
VERSION     DAXHTR010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.173"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            86..1315
                     /gene="sstT"
                     /locus_tag="ACFNML_001882"
     CDS             86..1315
                     /gene="sstT"
                     /locus_tag="ACFNML_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFC6393197.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIVLYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            1427..2707
                     /locus_tag="ACFNML_001883"
     CDS             1427..2707
                     /locus_tag="ACFNML_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HFC6393198.1"
                     /translation="MFERSVIAMACIFALSACGGGGGGSPDVKSADTPSKPAAPVVAE
                     KETEVKEDAPQAGSQGQGAPSTQGSQDMAAVSAENTGNGGAATTDKPKNEDEGPQNDM
                     PQNAGNTAAQGTNQAENNQVGGSQNPAPSSNPNATNGGNFGRVDLANGVLIDGPSQNI
                     TLTHCKSDSCNGDNLLSEEAPSKSEFEQLSDEDKIKKYKKDGEKFTGLVADRLQMKGT
                     NQYIIFYKPKTTSSARFRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSGNI
                     FAPEGNYRYLTYGAEKLSGGSYALSVQGEPAKGEMLAGTAVYNGEVLHFHTENGRSYP
                     TKGRFAAKVDFGSKSVDGIIDSGDDLHMGKQKFKAAIDGNGFKGTWTENSGGDVSGKF
                     YGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(2782..2997)
                     /locus_tag="ACFNML_001884"
     CDS             complement(2782..2997)
                     /locus_tag="ACFNML_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393199.1"
                     /translation="MFKLGVYACLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVFGQYFSEKKMKDDGFIN"
     gene            complement(2990..>3078)
                     /locus_tag="ACFNML_001885"
     CDS             complement(2990..>3078)
                     /locus_tag="ACFNML_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HFC6393200.1"
                     /translation="VTMEKDLRRMLLFFKREAYVVILERDRV"
BASE COUNT          746 a          744 c          881 g          707 t
ORIGIN      
        1 acagccgaaa aataaaaaaa cacagagaat gagttttctt ttaaaataca acatttttta
       61 acatatcaca acataaggaa gaattatggc gttcggcaaa tccttatttc atgcgatcgg
      121 ccgcgtcagc ctggtcaggc agattgccgc cggtttggcg ttgggcatcg taatcggttc
      181 ggtttccccg caactgggct tggcggcagg cttgttcggc agcctgtttg tcggcgcgct
      241 caaagcggtc gcgccggttt tggtatttat tttggtggcg gccacaatcg cgcagcacca
      301 aaaaggcaac aaggcgcata tcaggccgat tatcgtcctt tacctcatcg gtacgttttc
      361 cgcagccctg accgccgtca tcgccggtat ggttttcccg acgcacattg ttttggcggg
      421 cgcgggcgat gtgtccgccg cgccgccttc cggcattgtg gaagtgttga aatcgctgct
      481 gatgaacctg gtcgccaacc cgattaacgc gattgccaat gccaactata tcggcattct
      541 ggcttgggct ttggttttgg gcgcggcgtt gcggaatcac ggttcggacg ttacgcggca
      601 ggtcgttgcc gatttggcgg aagcggtttc caccgtcgtg aaatggatta tccgttttgc
      661 ccctttgggc attttcgggc tggtgtcgtc cacaatcgcg gagacgggtt tcggcgcgct
      721 ggcgggttat gcgaagctgc ttgcggtgct gttgggctgt atggcgttta tcgcgctagt
      781 ggtcaatccc gccatcgtgt ggtggaaaat ccgccgcaac ccttatccgc tggtgtttac
      841 ctgcctgcgc gaaagcggcg tgtatgcctt ctttacccgt tcttccgccg ccaatatccc
      901 cgtgaatatg gctttggcga aaaaactggg gctgcacgaa gacacttatt cgatttccat
      961 cccgttgggg gcgaccgtca atatgggcgg cgcggcgatt acgattaccg ttttggctat
     1021 ggcggcggcg cacacccaag gcatacaggt tgattttgcc accgcgctgc tgttgagcct
     1081 ggtggcgacg gtcagcgcgt gcggcgcgtc cggcgtggcg ggcggttcgc tgctgctgat
     1141 tccgctggcg tgcagcctgt tcggcatcag caacgatgtc gccatgcagg tggttgccgt
     1201 cggctttatc atcggcgtga ttcaggattc ggcggaaacg gcgttgaatt cttcaaccga
     1261 cgttttgttt accgctgctg ccgatttggg ccgacagaga aaccgggcgg aataaaccaa
     1321 gctatgccgt ctgaagccca tttggcgttc agacggcata tttttacaat tttatgggca
     1381 tatggcggaa acaataccgg ctgccttgaa tatgaaggag atgatgatgt ttgaacgcag
     1441 tgtgattgca atggcttgta tttttgccct ttcagcctgt gggggcggcg gtggcggatc
     1501 gcccgatgtt aagtcggcgg acacaccgtc aaaaccggcc gctcctgttg ttgctgaaaa
     1561 agagacagag gtaaaagaag atgcgccaca ggcaggttct caaggacagg gcgcgccatc
     1621 cacacaaggc agccaagata tggcggcagt ttcggcagaa aatacaggca atggcggtgc
     1681 ggcaacaacg gacaaaccca aaaatgaaga cgagggaccg caaaatgata tgccgcaaaa
     1741 tgccggcaat acggctgccc aaggtacaaa tcaagccgaa aacaatcaag tcggcggctc
     1801 tcaaaatcct gccccttcaa gcaatcctaa tgccacgaat ggtggcaatt ttggaagggt
     1861 tgatttggct aacggtgttt tgattgacgg accgtcgcaa aatataacgt tgacccattg
     1921 taaaagcgat tcttgtaatg gtgataatct attaagcgaa gaagcaccgt caaaatcaga
     1981 atttgaacaa ttaagtgatg aagacaaaat taagaaatat aaaaaagatg gagaaaagtt
     2041 taccggtttg gttgctgata ggttacagat gaaaggaacc aatcaatata ttatttttta
     2101 caaacctaaa accacttcat ctgcgcgatt caggcgttct gcacggtcta ggaggtcgct
     2161 tccggctgag atgccgctga tccccgtcaa tcaggcggat acgctgattg tcgatgggga
     2221 agcggtcagc ctgacggggc attccggcaa tatcttcgcg cccgaaggga attaccggta
     2281 tctgacttac ggggcagaaa aattgtccgg cggatcgtat gccctcagtg tgcaaggcga
     2341 accggcaaaa ggcgaaatgc ttgctggcac ggccgtgtac aacggcgaag tgctgcattt
     2401 ccatacggaa aacggacgtt cgtacccgac caaaggcagg tttgccgcaa aagtcgattt
     2461 cggcagcaaa tctgtggacg gcattatcga cagcggcgat gatttgcata tgggtaagca
     2521 aaaattcaaa gccgccatcg atggaaacgg ctttaagggg acttggacgg aaaatagcgg
     2581 cggggatgtt tccggaaagt tttacggccc ggccggcgaa gaagtggcgg gaaaatacag
     2641 ctatcgcccg acagatgcgg aaaagggcgg attcggcgtg tttgccggca aaaaagagca
     2701 ggattgatgc tgatgccgcc tgaagcgggg gcgttcagac ggcattgtga tgtttggggt
     2761 ggggtccggt tcaagtccgc atcagttgat aaacccgtcg tctttcattt tcttttcgga
     2821 aaaatactgg ccgaatacca gcagggcagc gaggatgatg acaaacagtc ccgccatagt
     2881 cagagtgatt ttgaaaaaca attccgcttc gagaaaagaa aaccaaagtt gcacaatcag
     2941 cagcagcacc cagccggcaa acagtccgag acaggcataa acgccgagct taaacacggt
     3001 cccgctccaa aatgacgacg taggcctcgc gtttgaaaaa cagcagcata cggcgcaaat
     3061 ctttttccat cgttacga
//
LOCUS       DAXHTR010000174         3050 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.174, whole genome shotgun sequence.
ACCESSION   DAXHTR010000174 DAXHTR010000000
VERSION     DAXHTR010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.174"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(471..1007)
                     /locus_tag="ACFNML_001886"
     CDS             complement(471..1007)
                     /locus_tag="ACFNML_001886"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HFC6393201.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEEKVVLVRQWRYAANQATLELPAGKLDVAGEDMAACALRELAEETPYTTD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(1041..1397)
                     /gene="folB"
                     /locus_tag="ACFNML_001887"
     CDS             complement(1041..1397)
                     /gene="folB"
                     /locus_tag="ACFNML_001887"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HFC6393202.1"
                     /translation="MDKIFLHGIKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            1453..2055
                     /gene="plsY"
                     /locus_tag="ACFNML_001888"
     CDS             1453..2055
                     /gene="plsY"
                     /locus_tag="ACFNML_001888"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFC6393203.1"
                     /translation="MFNIPAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAVLTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIFATLAIAILVLLRHKSNILNLIKGKESKIGGSR"
     gene            2132..2977
                     /locus_tag="ACFNML_001889"
     CDS             2132..2977
                     /locus_tag="ACFNML_001889"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HFC6393204.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSEAQKVGEYRQGIALLRQAKNLPTELNGFIAHIQKILPSPELYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLQNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
BASE COUNT          754 a          927 c          651 g          718 t
ORIGIN      
        1 gagctaaggc gaggcaacgc catactggtt tttgttaatc cactataccg ttctcaaatt
       61 caaattgggc ttttaggtct ataacaaaga aaaagccttg gcaggataaa cctaccaagg
      121 ctttttatta aaaaaaacgc tctattactg tgtctaaaaa tgcaaaaaaa gtaaccatca
      181 taaccctttt gccaaaatca atatcatctt ccgatgaatg aattgacggt atataagaaa
      241 gaattaataa gctcataaac cggtaagaaa aatcagtaac aaagattttc attattaacc
      301 attcttaaaa atgatcacgt tcgtttgggt aattattata cccttttccc tcttattact
      361 aaagcacgaa tgactttaaa ggacgcgccc agagacaagc cgaaaaggca tatctcatcc
      421 gccaaacaaa atgcccgcca atctactccg gagggcagtt caacaccctg tcaatccttc
      481 atcagccagt attgcaaacc gattaatgtc ttgccgtctt taatttcatc gtttgccaat
      541 gcctgacgga cttcttcttt cgacatcaat acggtttccg taatctcgtc ttcgtcattg
      601 gcaagcgtac tgcccaaacg cacgccttcc gcctcgaaca gatacatttt ttcgttgcaa
      661 aaaccgaccg ccgtataaaa actgtaaagc aggcgtacgc tgtcggtggt ataaggcgtt
      721 tcttcagcca gttctcgtaa agcgcatgct gccatatcct cgcccgccac atccagcttg
      781 cccgcaggaa gttccaatgt cgcctgattt gccgcatacc gccactgccg taccaaaacg
      841 accttttcct catccgttac cgccaacacg cacgccgcgc ccggatgccg gatgacgata
      901 cgctgccctt cattgccgtt gggcaacctg accttatccc tgctgatgct gacgaaaccg
      961 ccctcgtaaa tggtttcgcc gcctaatttt acttccctca aatccacttc atatctcctt
     1021 tccgtttcct attctgccgt tcaatcttca cgcttgccgc gctcgatttc cacgccaacc
     1081 tcgcgcacgc cttccaaaat acccggcttg acgattttca cgcgcaccca caccgcgccg
     1141 aaatatccca aaaccaaatc ggcaatatat tccgccaacg cttccaaaag caggaaatcc
     1201 tgttctttca gatgtcggcg cagcgtttcg catacctcgg cataatgcac cgtattggca
     1261 atatcgtcgt ccgaacccgc tttctcggga acgccgatgt ccaaatcgac aatcagggtc
     1321 tgcaaccgtt cgcgctccca gccgtacacg ccgataagcg tatccgcctt gatgccgtgc
     1381 aaaaagattt tatccataat ccgccccgtt ttttgctaaa ataaccgcac cattctaagt
     1441 aaagtaccgc aaatgttcaa cataccggct gttgctgttt cctatctgat cggctcactt
     1501 tctttcgccg tcatcgtgtc caagtattac ggcatggacg acccgcgcac ctacggatcg
     1561 ggcaatccgg gggcaaccaa tgttttacgc agcggcaaaa aaaaggcggc cgtgctgacg
     1621 ctcttgggcg atgccgccaa aggtttggtt gccgttttgc ttgcacgcgt gcttcaagaa
     1681 ccgctcggtt tatccgacag cgcaatcgct gccgtcgcac tcgccgcgct ggtcggacat
     1741 atgtggccgg tgtttttcgg atttaaaggc ggcaaaggcg tggcaacggc attgggcgtg
     1801 cttctggcac tctctcccgc aactgccttg gtctgcgcgt tgatttggct tgtgatggca
     1861 ttcggcttca aggtgtcctc ccttgccgca ttaaccgcca caatcgccgc cccccttgcc
     1921 gcactgtttt ttatgccgca tacttcttgg attttcgcaa ccctcgcaat cgccatattg
     1981 gtgttgctcc gccataagag caacatcctc aacctgatta aaggcaaaga aagcaaaatc
     2041 ggcggcagcc gctgatttcc catccatccc tgtttatgcc gtctgaaccc cgcttcagac
     2101 ggcatccgaa tatcccgccg ttttaccgac tatgctcagt tacagacacg cattccacgc
     2161 cggcaaccac gccgatatgc tcaaacactt caccttgttt ttagtgctgc aatatttcaa
     2221 ccgcaaagac aagccctact ggtacatcga cacgcacggc ggcgcgggtg tgtacaatct
     2281 cgaaggcagc gaggcacaaa aagtcggcga ataccggcaa ggcatcgccc tacttcgaca
     2341 agcaaaaaac ctgccaaccg agttaaacgg ctttatcgcg cacatacaaa aaatcctgcc
     2401 ctcgcccgaa ctttactgcg gttctccgtg gctggcgcaa tcgctgaccc gcgtcggcga
     2461 caaattacgc ctgtttgagc tgcaccccac cgactttgtc catctgcaaa acaatatggg
     2521 cgaagcagga ttgggcaaac gcggacaagt gttgcgcgaa gacggttaca aagggctgat
     2581 ttccctgctg ccgcccccgc cgcgccgcgc caccgtcctc atcgacccgc cctatgagga
     2641 aaaacaggac taccggcgtg taacggaaac gctgaaagcc gctttaaaac ggtttgagtc
     2701 cggctgttat ctcatttggt atccctgcct cagccgcgaa gaaagccgca agctgcccga
     2761 agaattgaaa aaactcctgc ccgacaacta ccttcacgcc gaacttcacg tccacgcgcc
     2821 gaaagccgac ggtttcggta tgcacggcag cggaatgttc gtcatcaatc cgccctatct
     2881 gttggcggaa caattggcgg caaacctccc cgccctgacg cggctgctgg cgcaagacga
     2941 aggcgcgcgc taccttttgg acagcaaaat ccgctgaccc cgatgccgtc tgaaaccctt
     3001 cagacggcat ctcccatcaa acaaaaccgg acgatatacc ggcagccggc
//
LOCUS       DAXHTR010000175         3002 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.175, whole genome shotgun sequence.
ACCESSION   DAXHTR010000175 DAXHTR010000000
VERSION     DAXHTR010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.175"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(63..2228)
                     /gene="fetA"
                     /locus_tag="ACFNML_001890"
                     /gene_synonym="frpB"
     CDS             complement(63..2228)
                     /gene="fetA"
                     /locus_tag="ACFNML_001890"
                     /gene_synonym="frpB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021438799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor FetA/FrpB"
                     /protein_id="HFC6393205.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDDDHRIVLSHMKDQHRGIRTVREEFTVGDKSSRINIDR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGANFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNSKFEISDKKKGADGKEVD
                     VDDAQKEKNRANEKIVHAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTH
                     DGKTVSSSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGT
                     KAERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNH
                     GYELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFK
                     NPNLEIGWRGRYVQKAVGSILVAGQKDRSGKLENVVRQGFGVNDVFANWKPLGKDTLN
                     VNLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          662 a          699 c          857 g          784 t
ORIGIN      
        1 aaattatcag aacagatgcc gtctgaaagg ctttcagacg gcattttttc gggatgtgcg
       61 ttttagaact tgtagttcac gcccaagcgt acatcgcggc ccacgcccgg cagggtattg
      121 gtccagcgtt ggctgtgcgg atagtagaac gtgttgaaca cgttgttaac cgaaagatta
      181 acattgagcg tgtctttgcc cagcggtttc cagttggcga agacatcgtt cacaccgaaa
      241 ccttggcgta caacgttttc caatttgccg ctgcggtctt tttgacctgc caccaatatc
      301 gaacccacgg ctttttgaac atagcgtccg cgccagccga tttccagatt cgggtttttg
      361 aagcggtagg caagggaggc tgtccaagtg cggccgactt gtgcgccaaa ttcaggattc
      421 gcgctcaaca gcttgtcttt gtgcgtatcg taaaagcgcg gtttgctgtg gcttacgccg
      481 actttggcag tcaggccgcc ggtgcggtag gacgcgccca attcgtaacc gtggtttttg
      541 atgtaaccgg cattgacggc ttcacggaca gcggcagaga cgtggcggtt ttgcggattg
      601 gcaagcgcgt ctttgatggt ttgccagaag tagctgccgt tggcggcaaa cgtgccgtcg
      661 ttgtagttga agccgatttc ggtattgcgc gcgcgttcgg ctttcgtgcc gtcggcaatc
      721 gagatgatgc cgcgtttacc gtgggtttgc agcgcgtcat acaggcgcgg gctgcggctg
      781 gcgtagttgt ggctcgcgct gaagctccag tgttcgtgcg gctgccaaat cacaccgaaa
      841 ctcgggttaa ggttgctgct tgaaacggtt ttgccgtcat gggttttcac cttgaagcgg
      901 tcgtaacgca gcccgccggt cagggtaaag ccgtcaatct cgtgaatggc ttcgatatac
      961 gcgccggtat cggttttggt cgggttagac agtttgtagg cgtgaacaat tttttcattg
     1021 gcacgatttt tttctttttg tgcatcatct acgtcgactt cttttccgtc ggctcctttt
     1081 ttcttatcgg agatctcaaa tttcgagttc aaaaacgctt gcggtttgat ttcctgatgg
     1141 cggtagttga taccgtattt cagcagggtt tgttcggcaa ggcggctgtc gaagttgaag
     1201 ttcgcaccac gagtggtgat tcgggtatgg ttggggcctt ttacattgcc tgcgtagccg
     1261 gtgccgctgt catcggcgga atagcgttct ttttccaaca cataggcgtt ggcatccagt
     1321 ttttcgacaa agcccaggtt tttacccgtg tacgccaagt tggtgttgga ttgggtagtt
     1381 tcgcggtaag caggggcttg gcggtcaata tttatccgtg aacttttgtc gccgacggta
     1441 aattcttcac gcacagtgcg gatgccccgg tgttggtctt tcatgtggct caacacgatg
     1501 cggtggtcgt cgtcgccgaa agttgttccg attttggcga ggtagctgcg tttgtccagc
     1561 gcgctgtacg gtacggtttt gccgccgttg acattgcgga aacctttgcc ggcttcgtaa
     1621 tctttttcat cgttgcggtt gtaagagaac aaaccgtcga agttgccctc ttttccgaat
     1681 acgctggcgc cgtagcttac gccttcgttg ctggcaaagc cgctgttgag gcgcacgccc
     1741 cagtttttat ccaagccttt gagcaggtct tgggcatcga cggttttggc gataatcgcg
     1801 ccgttggtcg cgccgatacc ggcagaggcg gaacccgcgc ctttttgtac ggaaacgact
     1861 ttaaccaaag cgggatcgac aataaatctg ccttggtggt aaaggatttg gctgtcggaa
     1921 taggcgttgt ccaccttgat gtcgacagag ttctgaccca taccgcgcag cgtcaggaat
     1981 tgggacgtgc cgttgccgcc gccgaaatcg atggacggct cttcttttaa gagttcgcgc
     2041 atatcggttg cggtgctttc gtctttttgt tgaagcgtaa cgatgttggt acggattttg
     2101 ctgccttggc ggtcgccttt tacggtaacg gtatccaatg cgacattggc attattttct
     2161 gccgcatggg caaaacctgc cgccagggta agcgagagca ggctgaggcg gaaaaacggg
     2221 gcgttcattt gttcgtcctt ttgagtgtat gaagggaagt aaatccaaac cgttaaggtt
     2281 tggcaggata agaaaaataa ttaataatta tttttattta tattaacagg ggggggggat
     2341 ttttgcaaag ctgattatcg tttttatttg cgaagtgttg ttttttgttg acaagttttg
     2401 tcggaaatgt aaaaaacggc ggaaatatag tggattaaca aatgcgggaa tgacgaaaag
     2461 caacctttcc gcgtccttcc cgcgaaagcg ggaatctaga acccctaacg cggcaggaat
     2521 ctatcggaaa aaaccgaaac cgaacgaacc tagattcccg cctgcgcggg aatgacgagg
     2581 cctgcgcggg aatgacgaaa agcaaccttt ccgcgtcctt cccgcgaaag cgggaatcta
     2641 gaacccctaa cgggcaggaa tctatcggaa aaaaccgaaa ccgaacagac tggattcccg
     2701 cctgcgcggg aatgacgaag cctgcgcggg aatgacgaaa agcaaccttt ccgcgtcctt
     2761 cccgcgaaag cgggaatcta ggaactcaaa gctgcaagaa tttatcagaa acaactggaa
     2821 ctcaaaaaaa ctagattccc gcctgcgcgg gaatgacgaa gggttaaaga aatgatgaga
     2881 aacggcggta atttatcggg aataaccgaa aacgaacgga aatcaggaca aggcggcgaa
     2941 gccgcagaca gtacggatag gacggaaccg attcacttgg tgcttgagca ccttagagaa
     3001 tc
//
LOCUS       DAXHTR010000176         2998 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.176, whole genome shotgun sequence.
ACCESSION   DAXHTR010000176 DAXHTR010000000
VERSION     DAXHTR010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.176"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(7..615)
                     /locus_tag="ACFNML_001891"
     CDS             complement(7..615)
                     /locus_tag="ACFNML_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HFC6393206.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(679..1272)
                     /locus_tag="ACFNML_001892"
     CDS             complement(679..1272)
                     /locus_tag="ACFNML_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HFC6393207.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAGKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFDHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAILKDT
                     DVLLNVPHPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(1277..1624)
                     /locus_tag="ACFNML_001893"
     CDS             complement(1277..1624)
                     /locus_tag="ACFNML_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFC6393208.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGAAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GSRPPEWIQNITG"
     gene            1670..2545
                     /gene="rsmI"
                     /locus_tag="ACFNML_001894"
     CDS             1670..2545
                     /gene="rsmI"
                     /locus_tag="ACFNML_001894"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFC6393209.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGQLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLARRVREVGFKVVPVVGASAVMAALSVAGVAGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELFPERRLMLAREITKTFETFLSGTVGEIQT
                     VLSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYDLALSWKNK"
     gene            complement(2580..>2998)
                     /locus_tag="ACFNML_001895"
     CDS             complement(2580..>2998)
                     /locus_tag="ACFNML_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFC6393210.1"
                     /translation="EAEWVGSLFGLHKSFGFLTLTVITLRIVWAVANRAKRPQSDSKA
                     AAAGHGILYLLMLAVPVIGMIRQYGSGRGPLKVFGVEVMQGSPEKIEWMANLGNTFHG
                     NLGWLLFAAVVGHVAMVVVHRVQGKDVLYRMTGRVR"
BASE COUNT          586 a          767 c          938 g          707 t
ORIGIN      
        1 gccgagtcag cgttggacgt agttttggta gagggtgatg actttttgta cgccgacggt
       61 ggtgctgact ttttgggtaa tctgcgcctg ttcttcgggg gtgaggatgc ccataacgta
      121 ggttacgttg ccgtaggtaa cgattttgac gcgcgcctgt gtggcggggc tgatgcccaa
      181 cagcgtggcg cggactttgg atgtgttcca agtgtcgccg gcgatgtcgc cggcagtgcg
      241 cggcagggag gcgacggtaa tgtagttgta tacgccttcg gcggcctgtt cggaacgtgc
      301 aatctgaccg acgaactgtt tctcgccttc ggtggcgact tgtccgagca gcagcaggtg
      361 gcggttgtag ccgacaacgg agatttgggg cgtgtagcct ttggtttggt tgttttggcg
      421 cagataggag cgggcggtgg tttcgatacg caacgccata acgttgtcgt cggtttgcgc
      481 gccggtggtt cggcggtcga cggcggattt cgcgccgacg gcggcgcttc cgattactgc
      541 gctgacgcag ccgctaaggg caaggctgaa aatggcggca atcagggtgc ggacggtgtg
      601 cggtttgggt ttcatcgggt gcttcctttc ttgggcgttt cagacggcat tgctttgcgc
      661 catgccgtct gaaaagggtt acattccttc cagcagtacg gagtcgatac agtcgcacat
      721 ggcgtgtatc agcaggatgt ggttttcttg aatacgggcg gtgcgcggat ggggaacgtt
      781 gagcaaaacg tcggtgtctt tgagtatggc ggcgattttg ccgccgtcgc ggccggtcaa
      841 ggcgatgacg tgcatatcgc gttcgtgtgc ggctttgacg gcttcgatga cgttggcgga
      901 attgccggag gtggaaatgc cgaccaatac atcgcctgca cgtccgagcg cgcgcacctg
      961 tttgctgaat acgtggtcga aaccgtagtc gttgccgatg gctgtcagcg cggaagtgtc
     1021 tgttgtcagc gcgacagcgg cgagttccat gcgttctttt tcaaaacggc cggtcatttc
     1081 ggcggcgaag tgttgcgcgt cggcagccga accgccgttg ccgcaggcca ggattttgcc
     1141 gtcattcatc aggcattgca gcatcagctc ggcagcctgt acggtcggct cgaccaatac
     1201 ttttccggct tcctgcttgg cacggatgct ttcggcaaaa tgggcggcaa cgcgttcttg
     1261 taatgtcgtc atatcgtcaa cctgtaatat tctgtatcca ctcgggcggg cggctgcctt
     1321 cgatgagtac cgcgtcgagg cggcacggta cgtttgtcaa cctgttctgt tgcagataat
     1381 actctacact tcgttgcagt ttcaataatt tggatgggga aatgctgtat gcggcaccgc
     1441 cgaattgccg atttttgcgg tattttactt caacaaacag aatcatgccg ccgtttttga
     1501 caatcaggtc gatttcgccg taggcgcagt gccagttgcg ggcaagcagt gtgcagcctt
     1561 gggattggag gaaggcaagc gcggcatctt cccctgcctc gccctgtttg tggtttaggc
     1621 gcataatcgg tttaaccttt ataatgtttg tttttcagac ggcattctta tgtttcagaa
     1681 acatttgcag aaagcctccg acagcgtcgt cggagggaca ttatacgtgg ttgccacgcc
     1741 catcggcaat ttggcggaca ttaccctgcg cgctttggcg gtattgcaaa aggcggacat
     1801 catctgtgcc gaagatacgc gcgttaccgc gcagcttttg agcgcgtacg gcattcaggg
     1861 ccaactcgtc agcgtgcgcg aacacaacga acggcagatg gcggacaaga ttgtcggcta
     1921 tctttcagac ggcatggttg tggcacaggt ttccgatgcg ggtacgccgg ccgtgtgcga
     1981 cccgggcgcg aaactcgccc gccgcgtgcg tgaggtcggg tttaaagttg tccctgttgt
     2041 cggcgcaagc gcggtgatgg cggctttgag tgtggctggt gtggcgggat ccgattttta
     2101 tttcaacggt tttgtaccgc cgaaatcggg cgaacgtagg aaattgtttg ccaaatgggt
     2161 gcgggcggca tttcctatcg tcatgtttga aacgccacac cgaatcgggg caacgcttgc
     2221 cgatatggtg gaattgttcc ccgaacgccg attaatgctg gcgcgcgaaa ttacgaaaac
     2281 gtttgaaacg ttcttaagcg gcacggttgg ggaaattcag acggtattgt ctgccgacgg
     2341 caaccaatcg cgcggcgaga tggtgttggt gctttatccg gcgcaggatg aaaaacacga
     2401 aggcttgtcc gagtccgcgc aaaacatcat gaaaatcctc acagccgagc tgccgaccaa
     2461 acaggcggcg gagcttgccg ccaaaatcac gggcgaggga aaaaaagctt tgtacgatct
     2521 ggcactgtct tggaaaaaca aatgatgccg gacggaccgg caccatagtg cgaacggaat
     2581 caacggacac gccccgtcat gcggtacagc acgtctttgc cttgaacacg gtggacgacg
     2641 accatggcga cgtgtccgac gacggcggca aacagcagcc agcccaaatt gccgtggaac
     2701 gtgttgccca agtttgccat ccactcgatt ttttccggcg aaccctgcat cacttcaacg
     2761 ccgaacactt tcaacgggcc gcggccgctg ccgtattggc ggatcatgcc gataacggga
     2821 acggcaagca tgagcagata cagaatgccg tgtcctgccg ccgcagcctt gctgtcgctt
     2881 tgcggacgct tggcgcggtt ggcaaccgcc cacacgatgc gcaatgtaat caccgtcagc
     2941 gtaaggaaac cgaaagattt gtgcaggccg aacaggctgc ccacccattc cgcctctt
//
LOCUS       DAXHTR010000177         2995 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.177, whole genome shotgun sequence.
ACCESSION   DAXHTR010000177 DAXHTR010000000
VERSION     DAXHTR010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.177"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            82..243
                     /locus_tag="ACFNML_001896"
     CDS             82..243
                     /locus_tag="ACFNML_001896"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393211.1"
                     /translation="MRWLNLKNSTSVHNTQGIRTKEAVEQGLRGNNRPEKGIGNRWNY
                     DGFIKELAK"
     gene            293..577
                     /locus_tag="ACFNML_001897"
     CDS             293..577
                     /locus_tag="ACFNML_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393212.1"
                     /translation="MKVITITLFTSCILSGCYGLEYGMSGGIPHDDWAIGSHVSQAKA
                     DAAWKECSDKYMRNGGSGLVEQCMNDKGLIRLSEIRRRQKRAETQRAVIK"
     gene            complement(702..1418)
                     /gene="trmB"
                     /locus_tag="ACFNML_001898"
     CDS             complement(702..1418)
                     /gene="trmB"
                     /locus_tag="ACFNML_001898"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFC6393213.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPADLDANFGSNRPKILEIGFGMGTATAEIARRLPETDFLAIDVHGPGVG
                     NLLKLIEENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTAADYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIG"
     gene            complement(1540..2943)
                     /locus_tag="ACFNML_001899"
     CDS             complement(1540..2943)
                     /locus_tag="ACFNML_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFC6393214.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLISLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDKAVYRFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGIAPDTEKARHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT          627 a          622 c          902 g          844 t
ORIGIN      
        1 atacgattaa agtaatgtcg gattcaagaa tccgacctac ggaaaactaa agattttata
       61 cgggctacgg cttgctggct tatgcgttgg cttaatttga aaaacagcac atcggttcat
      121 aatacacaag gtattagaac aaaagaagca gttgaacagg gattgcgtgg taataataga
      181 cctgaaaaag gaataggtaa tagatggaat tatgatggat ttataaagga attagcgaag
      241 taacaggtat tccaaatatt tctgataaat ataaagaaca aggagtaaaa aaatgaaagt
      301 aatcactatt acattgttta ctagctgcat actatctgga tgctatgggc ttgaatatgg
      361 aatgagtggc ggcattcctc atgatgactg ggcaataggt agccatgtta gtcaagccaa
      421 agcagatgct gcgtggaaag aatgttctga caaatatatg agaaatggtg gttcaggatt
      481 agtagaacaa tgtatgaatg ataaaggatt aatacgttta tctgaaatac gtcgtaggca
      541 aaagagagca gaaacacaaa gagcagtaat taagtaacaa taatgaaata ctactgttcc
      601 cgaacaaagc ggcatttacg caggtgatga cggctatatt ttgaaaaatg ccgtctgtaa
      661 gcaaacgtgc ttcagacggc atttttttat ccggcggtct gttatccgat ccgtttgaat
      721 accaagtccc aaacgccgtg tccgaggcgt ttgccgcgcg cttcgaattt ggtttcgggg
      781 cggtagtccg gggtgggggc gtagtctgcc gccgtatttt gcaggctgtc gaagctgctg
      841 aggacttcaa gcatctgctg tgcatattct tcccagtctg tcgccaggtg gatatagccg
      901 ccggttttga gtttgggcag cagtttggcg atgaaggggg cttgtatcag acggcgtttg
      961 tggtggcgtt ttttgtgcca cgggtcgggg aagaatatgt ggatgccgtc gagcgagccg
     1021 tcttgcagca tattttcgac aacttctacg gcgtcgtgcc gcatcacgcg gatgttttcc
     1081 aaatggtttt cttctatgag tttgagcagg ttgcctacgc cgggaccgtg tacgtcgatg
     1141 gcgagaaagt cggtttcggg cagcctgcgg gcgatttctg cggttgctgt ccccataccg
     1201 aagcctattt cgaggatttt ggggcggttg cttccgaaat tggcatcaag atcggccggt
     1261 gttgcttggt aatcgatgcc gaagctgtcc cataaggtat cgatggcgcg ttgctgcgcg
     1321 gcggtcatat ggctttggcg gaggacgaaa ctgcggatgc tgcggggatg tccggcttga
     1381 cggtcgtttg gtgtttggtt ttcggcggtg tctgtcataa tgcttccgtg cgcttgtgca
     1441 atcaggatgc cgtctgaatg tgaaaggcct gttcggacgg cgggaaggga aaggggcgat
     1501 tatacctgtt tcgtttcggc agggtaggtg taacgctgtt cagacggcat tttgccattg
     1561 ttgcagcagt tcttgcagga cgcttttttg cccgtatccg ttgtttatgg cttcttgcag
     1621 gtagcggcag gctttttctt tgtccggccc gacaccgtgt ccgctgtaat ggatgcaggc
     1681 gaggttgtag aacgccatac tgtctttttt tgcggcggct ttttcaaacc atttgcgtgc
     1741 ttcattgtag tcggcggtca taccctgtcc gaagtaatac atgctgccca gattggtttg
     1801 ggctttgctg tgtccgagtt ctgcggcttc tgtgtagagt ttgagcgcgg ctgcgtattc
     1861 gggcgcgcat tgtaatccgt aatgcagggc ttgggctttt tgataaagct gttctgccct
     1921 ttgccgccta atggcggaat ctttaatgcc gccgtattgg tcaggatggt ttaacgcgct
     1981 gtcggatatg agtttctgat aggcggtaag gctgccggct tcggctgcct gccggtaata
     2041 atgccgggct ttttccgtat cgggggctat gcccaaaccg tagcggcaga tgtcgcccag
     2101 tatccgcaaa ccttcgggat ggcgttcggc ggcggctgct tcggcgtgat gcagggcaag
     2161 tttgtagttt ctttccggat gcttgccttg caggtagatg tcggcaagtg cggcatgggc
     2221 atcggggtca ttttcctgtg cggcctgttg aaaccggtaa acggctttat cgggagccgt
     2281 aggtaggatg agctcggcaa gcggggtgta ggcgggaatg tatccttgtt gtgcggcggc
     2341 gcgcaaatga ttgatagctt gggtcgtgtc ggttggtgta ccttgtccga aacggtattg
     2401 aaggccgagc tgccattgtg ccgccgccaa gccttgtgct gcagcctcgc ggtagagcag
     2461 gtgtgccgtt tcaaaatccg gcggcgtgcc gtaccggcag tattgggcaa gcaggtattg
     2521 cccgtggggg ttgttgtttt ttgcgcttgt ttttgcccat ttcagggctt cttcaaaatg
     2581 tcgggacgca tacagcaggt acatcaggcg gacttgtgct tcaggcagcc cttcctctgc
     2641 taatggtctg taaagcgata tcagggtttc cgggggtgcg ccttccaatt cttggatata
     2701 tgccagattg taacgcgcgt agggatgtcc ctgttcggcg gcgatgcgga gcatttccat
     2761 accttgttcg cggtcttggt aggggctgtc cgcgtccatc aggtgttttg ccaactcgaa
     2821 tgcggcggcg ggatgacctg ccgaggccgc ttctgtcagc agtcggcgga cttcggtaaa
     2881 gttatattgt ttttgagaca ggcgttctaa ggcctgccga tagggtaagt cgggattgtt
     2941 catatcagat cgttttccgt tttgtcgaat catcgttatt gtgttatgaa gttag
//
LOCUS       DAXHTR010000178         2940 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.178, whole genome shotgun sequence.
ACCESSION   DAXHTR010000178 DAXHTR010000000
VERSION     DAXHTR010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.178"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            328..651
                     /locus_tag="ACFNML_001900"
     CDS             328..651
                     /locus_tag="ACFNML_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HFC6393215.1"
                     /translation="MQNETINPKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSVVLIELSD
                     PSEAV"
     gene            765..>2940
                     /locus_tag="ACFNML_001901"
     CDS             765..>2940
                     /locus_tag="ACFNML_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HFC6393216.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQESTELPTITVTAD
                     HTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLAGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          707 a         1050 c          740 g          443 t
ORIGIN      
        1 gctccgtttt agcttcgcag aaactccgct tccttcgaaa gctccgtttt cagacgacct
       61 tttggaacac cacaggcaca cgcatcgaac ggctgaatca aagttcagac cgatggcagc
      121 ccgcacccga gtttatgcgg caaacagcga ggctacggca acccgtcccc tctccctgtg
      181 ggagagggtt agggagaggg cggtaagccg caggcttaca tcaaagccga taacgcttcc
      241 gttacaactc cgcccactga aagcagcctg caacgaagcc aaaacgacaa accgcatcgt
      301 aaaccaccca acccatagga gaaccccatg caaaacgaaa ccatcaaccc gaaacagcac
      361 cttgccgcca tcaaagaata ctggcagccc gaaatcatca accgccacgg gttccaattc
      421 cgcttggtca aacttttggg cgattacggc tggcatacgc acggatacag cgacaaagtg
      481 ctgtttgccg tggagggcga catggcggtg gacttcgccg acggcggcag catgacgata
      541 cgcgagggcg agatggcggt cgtgccgaag tcggtgtcgc accgcccgcg ttcggaaaac
      601 ggctgctcgg tggtgctgat tgagttgtcc gacccgtccg aggccgtctg aaaacgaagt
      661 ttccgaagga agctgagttt ctgcgaagct aaaagcagcc tgcaccttca atcaatatgc
      721 cgaaaataca accccaccgc acaccaacac acaaaggaaa tcccatgaca cgcttcaaat
      781 attccctgct gtttgccgcc ctgttgcccg tgtacgcgca ggccgatgtt tctgtttcag
      841 acgaccccaa accgcaggaa agcactgaat tgccgaccat caccgttacc gccgaccaca
      901 ccgcgagttc caacgacggc tacactgttt ccggcacgca caccccgctc gggctgccca
      961 tgaccctgcg cgaaatcccg cagagcgtca gcgtcatcac atcgcaacaa atgcgcgacc
     1021 aaaacatcaa aacgctcgac cgcgccctgt tgcaggcgac cggcaccagc cgccagattt
     1081 acggctccga ccgcgcgggc tacaactacc tgttcgcgcg cggcagccgc atcgccaact
     1141 accaaatcaa cggcatcccc gttgccgacg cgctggccga tacgggcaat gccaacaccg
     1201 ccgcctatga gcgcgtagaa gtcgtgcgcg gcgtggcggg gctgctggac ggcacgggcg
     1261 agccttccgc caccgtcaat ctggtgcgca aacgcccgac ccgcaagcca ttgtttgaag
     1321 tccgcgccga agcgggcaac cgcaaacatt tcgggctggg cgcggacgta tcgggcagcc
     1381 tgaacaccga aggcacgctg cgcggccgcc tggtttccac cttcggacgc ggcgactcgt
     1441 ggcggcagcg cgaacgtagc cgcgatgccg aactctacgg cattttggaa tacgacatcg
     1501 caccgcaaac ccgcgtccac gcaggcatgg actaccagca ggcgaaagaa accgccgacg
     1561 cgccgctcag ctacgccgtg tacgacagcc aaggttatgc caccgccttc ggcccgaaag
     1621 acaaccccgc cacaaattgg gcgaacagcc gccaccgtgc gctcaacctg ttcgccggca
     1681 tcgaacaccg cttcaaccaa gactggaaac tcaaagccga atacgactac acccgcagcc
     1741 gcttccgcca gccctacggc gtagcaggcg tgctttccat cgaccacaac accgccgcca
     1801 ccgacctgat tcccggttat tggcacgccg acccgcgcac ccacagcgcc agcgtgtcat
     1861 tgatcggcaa ataccgcctg ttcggccgcg aacacgattt aatcgcgggt atcaacggtt
     1921 acaaatacgc cagcaacaaa tacggcgaac gcagcatcat ccccaacgcc attcccaacg
     1981 cctacgaatt ttcccgcacg ggtgcctacc cgcagcctgc atcgtttgcc caaaccatcc
     2041 cgcaatacgg caccaggcgg caaatcggcg gctatctcgc cacccgtttc cgcgccgccg
     2101 acaacctttc gctgatactc ggcggcagat acagccgtta ccgcaccggc agctacgaca
     2161 gccgcacaca aggcatgacc tatgtgtccg ccaaccgttt caccccctac acaggcatcg
     2221 tgttcgacct gaccggcaac ctgtctcttt acggctcgta cagcagcctg ttcgtcccgc
     2281 aatcgcaaaa agacgaacac ggcagctacc tgaaacccgt aaccggcaac aatctggaag
     2341 ccggcatcaa aggcgaatgg cttgcaggcc gtctgaacgc atccgccgcc gtgtaccgcg
     2401 cccgtaaaaa caacctcgcc accgcagcag gacgcgaccc gagcggcaac acctactacc
     2461 gcgccgccaa ccaagccaaa acccacggct gggaaatcga agtcggcggc cgcatcacgc
     2521 ccgaatggca gatacaggca ggttacagcc aaagcaaaac ccgcgaccaa gacggcagcc
     2581 gcctgaaccc cgacagcgta cccgaacgca gcttcaaact cttcactgcc taccactttg
     2641 cccccgaagc ccccagcggc tggaccatcg gcgcaggcgt gcgctggcag agcgaaaccc
     2701 acaccgaccc tgccacgctc cgcatcccca accccgccgc caaagcccgc gccgccgaca
     2761 acagccgcca aaaagcctac gccgtcgccg acatcatggc gcgttaccgc ttcaatccgc
     2821 gcgccgaact gtcgctgaac gtggacaatc tgttcaacaa acactaccgc acccagcccg
     2881 accgccacag ctacggcgca ctgcggacag tgaacgcggc gtttacctat cggtttaaat
//
LOCUS       DAXHTR010000179         2910 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.179, whole genome shotgun sequence.
ACCESSION   DAXHTR010000179 DAXHTR010000000
VERSION     DAXHTR010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.179"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            43..1260
                     /locus_tag="ACFNML_001902"
     CDS             43..1260
                     /locus_tag="ACFNML_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFC6393217.1"
                     /translation="MTHTASKTPKFWLTITAAAFILLITIGMRMTLGLFVQPVVNTTE
                     LSIAQFSLIIAVFQLMWGISQPLSGALADRFGAFRVLSGGAVLLVCACLIAPNIPTYW
                     GLMIAVGLLLAFGTGSGGFSIIMGQVAAQVPAHKRGLASGLVNAGGSAGQFLFAPLVQ
                     GLVVLPEVGWTGTFYVWGAIALLILPVSWWLAGGNNAAHTQHAQATHGQSLGEAVKTA
                     FKTPSYILLHLSFFACGFHIAFLVTHLPTEVALCGLPATVASTSIAIIGLANIAGCIF
                     SGWCTSRVRGKYVLFGLYASRAAMILIYIFSPKTDLNFYIFAAALGFTWLATVTPTAS
                     ITGKLFGTRYLATLFGLTMLSHQIGGFLGSYIGGIVITQFGDYGWMWYADALLAGTAA
                     LLNLPIREPRTAA"
     gene            1257..1388
                     /locus_tag="ACFNML_001903"
     CDS             1257..1388
                     /locus_tag="ACFNML_001903"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393218.1"
                     /translation="MKECRLKTLRRHFPNTPPVSGLHDTQISLIKPQTTAAIKPPFK"
     gene            complement(1463..1603)
                     /locus_tag="ACFNML_001904"
     CDS             complement(1463..1603)
                     /locus_tag="ACFNML_001904"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393219.1"
                     /translation="MNYRIGNAVCIDEMPDTGRGGQTFAKRTASDASPARAGAASDGI
                     LS"
     gene            1616..2740
                     /locus_tag="ACFNML_001905"
     CDS             1616..2740
                     /locus_tag="ACFNML_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFC6393220.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDSRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGGDLERLAARDIIRFPGFVNNVSEVIKAHH
                     IFVLPSYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILAEKM
                     IYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT          684 a          869 c          683 g          674 t
ORIGIN      
        1 cactataaaa tccgcaaact taaccgactc aaaatacaca ttatgacgca caccgcatcg
       61 aaaacaccca aattttggct gaccatcacc gccgccgcat tcatcctgct gattaccatc
      121 ggcatgagga tgacgctcgg actgttcgtc caacccgtcg tcaacaccac cgaattgagc
      181 attgcccaat tcagcctcat catcgccgtt ttccaactga tgtggggcat atcgcaacca
      241 ttgtccggcg cgcttgccga ccgtttcggc gcgttcaggg tattaagcgg cggcgccgtc
      301 ctgctcgtct gcgcctgcct gattgccccc aacatcccga cttattgggg gctgatgatt
      361 gccgtcgggc tgctgctcgc attcggcacc ggttccggcg gtttctccat cattatgggg
      421 caggtcgccg cccaagtccc cgcacacaaa cgcggcttgg catccggact ggtcaacgcc
      481 ggcggttcgg caggacaatt cctgttcgca ccgctggttc aaggactcgt cgtcctgccc
      541 gaagtcggct ggacgggcac attttacgtt tggggcgcaa tcgccctgct gattctgcct
      601 gtctcatggt ggcttgccgg cggcaacaac gccgcccaca cccaacacgc ccaagccaca
      661 cacggacaaa gcctcggaga agcagtcaaa accgccttca aaaccccaag ctacatcctg
      721 ctgcacctaa gcttttttgc ctgcggcttc cacatcgcct ttctcgtaac ccacctaccc
      781 acggaagtcg ccctgtgcgg actgcccgcc accgtcgcct cgacatccat cgccatcatc
      841 ggactggcaa acatcgccgg ctgcattttt tccggctggt gcacaagccg cgttcggggc
      901 aaatacgtcc tgttcggact gtacgcctcg cgcgccgcga tgatactcat ctacattttt
      961 tcgcccaaga ccgacctcaa cttctacatt ttcgccgccg cactcggatt tacctggctc
     1021 gccaccgtca cgccgaccgc ctccattacc ggcaaactct tcggcacacg ctacctcgcc
     1081 accctgttcg gactgacgat gctcagccac caaatcggcg gattcctcgg ctcgtacatc
     1141 ggcggcatcg tgattacaca attcggcgac tacggctgga tgtggtacgc cgacgcactg
     1201 ctggcgggaa ccgccgccct cctcaacctc cccatccgcg aaccccgaac cgccgcatga
     1261 aagaatgccg tctgaaaacc ctcagacggc attttccaaa caccccgccc gtttccggac
     1321 tgcacgacac gcaaatcagc cttatcaaac cgcaaacaac cgccgcgatt aaaccgccat
     1381 tcaaataagt ccgttttccc ttcgggtgta aaatcatcac aaaacgcgat actgtatatc
     1441 gccgggcgtt atgcagcgga tttcaggata aaatgccgtc tgaagccgcc cctgcccggg
     1501 cgggcgaagc gtcggatgcg gtgcgcttgg caaaggtttg cccgccgcgc cccgtatccg
     1561 gcatttcatc aatgcagacc gcatttccaa tcctataatt caaggtgtcc gccttatgaa
     1621 aatcgttttt atcacaacag tcgcatccag catttacggt ttccgcgccc ccgtcattaa
     1681 aaaattaatc ggcaaaaacc atcaggtgta tgcctttgta tcggagtttt ccgataatga
     1741 attggatatt atcagggaaa tgggggtaac acccgttacc taccgttcaa accgcagcgg
     1801 gctgaacccg ttttccgata taaaatccac cttcctcatc tttaaagaac tcaaaaaaat
     1861 atcgccggat ttggttttcc cttatttcgc aaaacccgtg attttcggca cttttgccgc
     1921 aaaactggca ggcgtgccca gaatcgtcgg gatgctggaa ggtttgggat tcgcatttac
     1981 cccgcagccg gaaggcatac cgttaaaaac aaaaatcata aaggggattt tgattgcctt
     2041 gtaccacatt gccctgccga tgttggaaag cctgattgta ttaaaccccg acgacaaaga
     2101 cgaactgacg gacaaatacg gcatcaaaat aaaaaacatc catattttgg gcggaatcgg
     2161 tctggattcg cggcaatatc cttattccga ggcggatatt cccgatgaaa aagaacccgt
     2221 aaaattcctc tttatcggca gatttctgaa agaaaagggg attgatgatt ttattcgggc
     2281 ggcggaacag gttaaggaca aataccccga tacggttttt accgctttgg gcgcaatcga
     2341 caaatcacgc ggggggggcg atttggaacg gcttgccgcc cgcgatatta tccgtttccc
     2401 cggttttgtg aacaatgttt ccgaagtgat aaaggcgcat catatattcg tattgccgtc
     2461 ttatagggaa ggcgttcccc gaagcactca ggaggcaatg gccgtcggca gggcagtgat
     2521 tacgacggat gtccccggat gcagggaaac ggtcgccgac aaggtcaacg gcttcctgat
     2581 cgagccttgg aatccccgca tcttagccga aaaaatgatt tattttatcg aaaacaggga
     2641 agccgtccgc ctgatgggga atgcaagtta tgcgattgcc aaagataaat tcgatgccga
     2701 aaaagtcgat ttgaaattgc tcgatatttt gaaggcgtaa acaaggctgc ccgcttttga
     2761 gtttcggtca tttctgataa acccccgtca ttcccgccac acccccgtca ttccctcgaa
     2821 agcgggaatt caggttcgtt tagtttcggt catttccgat aaattcttgc aactttgcgt
     2881 ttctagattc ccactttcgt gggaatgacg
//
LOCUS       DAXHTR010000180         2905 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.180, whole genome shotgun sequence.
ACCESSION   DAXHTR010000180 DAXHTR010000000
VERSION     DAXHTR010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2905
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.180"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(389..1189)
                     /locus_tag="ACFNML_001906"
     CDS             complement(389..1189)
                     /locus_tag="ACFNML_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HFC6393221.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTMAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(1325..1828)
                     /locus_tag="ACFNML_001907"
     CDS             complement(1325..1828)
                     /locus_tag="ACFNML_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393222.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGWMPYFACYWFGLCYPVSTYYVFL
                     SRDYKHI"
     gene            complement(2165..2806)
                     /locus_tag="ACFNML_001908"
     CDS             complement(2165..2806)
                     /locus_tag="ACFNML_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HFC6393223.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
BASE COUNT          930 a          622 c          505 g          848 t
ORIGIN      
        1 ctcttttggg cttattcaaa ttagggacac gccctagaac caagttgcat gataatacct
       61 ttcattaact gaaacactga ttaagaaact ccagtctgtc taatgatgag gttttcacat
      121 cgccaaaact tgccaatcaa atgctggatt tattgccgtc tgagatttgg tcaaatccaa
      181 aggcgacatt cttagaccct gtgtgtaaat caggggtatt tttgcgtgaa atcgtcaaac
      241 gcttggatga aggcttgacc aatcaaatac cagataaaca aactcgcatt aaccacattt
      301 taaaaaatca agtttttgga agtactgcca cgtatgtagg tagctttgac cgatatttgc
      361 ataaaaactc ctttgctggt gaaaggaatt attttgccaa ttttaaaata tttctggcac
      421 caaatagtac aatgacaaag acaatcatgc caatgattaa atcaggatag ctagaatgag
      481 tcaataacgt caatgctccc gccgctatca caccgatatt gatgataatg tcattggatg
      541 taaaaatcat gctggctttg atatggattt ctttattttg atttttgctc agtagatata
      601 agcacagcca gtttgcaatc aatgccaaaa atgccgtgcc aatcatcagt tgataattgg
      661 gcagctgctc agcaccgata aaacgcctaa tcacttctat caccccaaat aacgccaata
      721 ttatctgcgt tatccccgcc aaaaatgcca cacgtttttt atacgccatc gtcataccaa
      781 tggctgatag cgccaatata tagacaaagc tgtccgccag catatctaga ctatcagcaa
      841 tcagccccat agaattagca aaaataccaa ccgaacactc tatgataaaa aacacaaagt
      901 taatcatgag cacttgatat aataatcttt tttctaagtg ctcatcaggc ttgttaaaca
      961 ctatcttatc aacaatcact tcggtggaaa tgatatgact atcaaaatta agcggttcaa
     1021 gtacttgtaa aatcgttgta tcttgattat cgtgatagac ggttaagcac cgcccagcaa
     1081 tatcaaactg taattcataa atatcagaca catcttttaa acgcatgcga atgagctgtt
     1141 cttcggacgg gcagtccatt ttggtaatgt taaaaatggt ctttttcatc tatttagttc
     1201 cttgttttga tcaggttggc tcaaataaat ctgtgtttat attgctgctt ggtaattttt
     1261 ggatggtttg agtaaattga ttaggttaaa atttaccttt ggaagtaccg ccacgcataa
     1321 tagtttagat atgtttataa tctctggata aaaaaacgta ataagtgctt actggataac
     1381 aaagtccaaa ccaatagcag gcaaaataag gcatccaccc cccttcttca ttaaggatat
     1441 atattgagaa acaaatcgca actaaacaga aaaaacttgg gagataaagc catttcattc
     1501 ccctattcaa gaatctagcc aagataggta ttttgtattc tacaaaaaag aaaggcattt
     1561 ccaagggaaa catgtcagat aaaaactttt gtttattttt tactatagat agaaccttgc
     1621 ttctcaagag aaagccatta ataataccga tgacagctat taatatatag agaatagtat
     1681 aagtatgaat aatcttcatt agacaaaaag aagaaatggc agataaatta catacgatat
     1741 attggaatat aaaatattta cggtctaaac cttgttcagt tgcaattttt ttaaaattgc
     1801 cttgcataaa aaaatcaaag gcgtccatta aactatcttt cacattagaa atttaaaagc
     1861 taaataatac gacaaacaat gtgaagtact attcatggtt tatttaaaaa ataatactat
     1921 tctgaacatt atttagatac agaaattaac aaattagaac taaacaagct tttaaatact
     1981 ttaattttat tggaaagcta taaaaggaac tataacttta cacactagtc acttcttttt
     2041 aagaggcaaa agggattggg aaggtcgtct tggagataag cactggtatt tcggccaatg
     2101 gtaaatagag tttacctcaa atagggtaga acctccttca tctgtcagtt aataacagcc
     2161 acttttacaa tgccctgtca aaataaagcg gcacgcccga tttttcactc atcgtcatca
     2221 aataacccat caccttttgg ggccattcga tgccgcgcac cacggtcaga ttcctcaaaa
     2281 cggggaaaac caaaatatcc tccataccga ttccgccgtt gatgccgtct gaagcaccgt
     2341 ccatcaaatt ttccaactct tgcaaatctg cgtttatgcg ttcgagatat tgggcggttt
     2401 tattcaaatt ggcggaaaag ctgccgatgc ttttctcttt tttgtctgta aaatatttta
     2461 ccgcttccgg cgttgcaaat tcgggcagcc cgattttgat cacgcgcggc tgcaccagtt
     2521 tgtcgttgta tccgcccacc ttgtccagcc acgcccgtat ctcggggcgg acttcgtctt
     2581 tcagacggtc ttcgcggtcg aaatgccgca caatgtccaa actctcgccc ataaacgaac
     2641 cgtcttcttt ttgcaggacg ggcacttgtt tcgcaccgat cataccgatc ggcgttgcct
     2701 cgtcgtcgtt tgccagcacg gcttcttcaa cgtccgcgcc aaacagcccg gcagccatcc
     2761 gcgcacgcac acaaaacggg caatggtcgt aaatatacag tttcatcaaa atattcctcg
     2821 tcaacctgtc ggtaccggct accttaacac cccgcgccac ccgaaacaag tttatcttcc
     2881 cgcctatgca ccgtaaataa ataag
//
LOCUS       DAXHTR010000181         2844 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.181, whole genome shotgun sequence.
ACCESSION   DAXHTR010000181 DAXHTR010000000
VERSION     DAXHTR010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.181"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(53..529)
                     /gene="greA"
                     /locus_tag="ACFNML_001909"
     CDS             complement(53..529)
                     /gene="greA"
                     /locus_tag="ACFNML_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFC6393224.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            921..2222
                     /gene="aroA"
                     /locus_tag="ACFNML_001910"
     CDS             921..2222
                     /gene="aroA"
                     /locus_tag="ACFNML_001910"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFC6393225.1"
                     /translation="MTESLRLPAASLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYRLHGVARMHERPIGDLADALRIAGADVEYLGKEHYPPLHIGERQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADAHYHAPEHLHVEGDASSASYFLAAGLIATTPVRVTGIGANSIQGDVA
                     FACELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAVVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVAEEAEAIHITPPETPTPDAVIDTYDDHRM
                     AMCFSLVSLLDVPVVINDPKCTHKTFPTYFEVFSSLTETAE"
     gene            complement(2265..2798)
                     /locus_tag="ACFNML_001911"
     CDS             complement(2265..2798)
                     /locus_tag="ACFNML_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFC6393226.1"
                     /translation="MFPPDKTLFLCLSALLLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTATGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
BASE COUNT          601 a          783 c          766 g          694 t
ORIGIN      
        1 ttaatccact ataatttcct gcgtgtgtcg ggtgtatcga aatcaagccg aatcaaatat
       61 atcggacttc gataatgtcg tattcgcgca cgccgcccgg ggcttggact tccgccgtat
      121 cgccctcttc cttgccgatt aaggcgcggg caatcggtga gccgacatag attttgccct
      181 gtttgatatc cgcttcgtct tcgccgacaa tttggtaggt aacgtgttct tccgtttcca
      241 aatcttccag cgtaaccgtc gtaccgaaca cgattttgcc ttcggcgtgg atttcggtcg
      301 gattgatgat gtgggcaacg gaaagtttgt gttccagctc ggaaatgcgg ccttcgataa
      361 agccttggcg ttctttggcg gcttcatatt cggcgttttc ggacaaatcg ccgtgcgatc
      421 tggcttcagc aatcgcttcg atcacttcgg ggcgcgccac gcttttaagc tgctgcaatt
      481 cctgtttcag caattccgca ccgcgtacgg tcagggggat tttttgcatc ggtctttttt
      541 ctccatattc cggcacaccg gtttgcggca gcaaacatac cgcataccgt cttgttttgt
      601 gcgtccggat attaaaataa aaacacaagc cgcccggaaa atcggcggct tgtctgtcgt
      661 tgaacagcgg ctattctacc aaattctatg aaattggcaa tcgtgccgtg ccgtccgcaa
      721 acacgctatg tccgcaacaa aagccgaaaa tatgccggca aaaaaatttt agaaacaaaa
      781 aatttaaaaa taatcaattt tcggcacaaa aaaccatatt cacggacttt aaaaccgaaa
      841 atgccaaacc tgagattttt cagacggcat ttgcaccagt ataatgcagg ctgtttttat
      901 ctttatattt acgttttgcc atgaccgaat ccctccgcct ccctgccgcc tcgctcaaac
      961 cttccactgt cgccctgccc ggctccaaaa gcatcagcaa ccgcaccctg ctgcttgccg
     1021 ccttgtccga caatgcttgc gaaatccatt ccctgctcaa atccgacgat accgaccgta
     1081 tgctcgaagc actcgataaa ctcggcgttg aaatcgaata tcttgccgaa gaccgtctga
     1141 aagtgcacgg cacaggcgga cgcttcccca accgcactgc cgatttgttt ttgggcaacg
     1201 cgggcacggc gttccgcccg ctgaccgccg cgcttgccgt tttgggcggc gattatcgcc
     1261 tgcacggcgt tgcgcgtatg cacgaacgcc ccatcggcga tttggccgat gcactgcgga
     1321 ttgccggcgc cgatgtcgaa tatctcggca aggaacacta tccgccgctt catatcggcg
     1381 aacgccaaga caacggcgag cgcgtgattc cgattaaagg caatgtatcc agccagtttc
     1441 tgaccgccct tttaatggcg ttgccgctga ccgggcaggc gtttgaaatc cgtatggtcg
     1501 gcgagttaat ttccaagccc tatatcgaca ttactttaaa actgatggcg caattcggcg
     1561 tacaggttat caatgaaggc taccgcgtct tcaaaattcc cgccgatgcg cactatcacg
     1621 cgcccgaaca cttgcacgtc gaaggcgatg cctccagcgc gtcctacttc ctcgcagccg
     1681 gtttgattgc caccacgccc gtccgcgtta ccggtatcgg cgcaaacagc atacagggcg
     1741 atgtcgcctt tgcctgcgag ctggaaaaaa tcggtgcgga cgtggtttgg ggcgaaaact
     1801 tcgtcgaagt ctcgcgcccg aaagaacgtg ccgtccaagc ctttgatttg gatgcaaacc
     1861 atatccccga tgccgccatg accctcgccg tcgtcgcgct tgctacaggg caaacctgca
     1921 cgcttcgcaa catcggttcg tggcgcgtca aagagaccga ccgcattgcc gcaatggcga
     1981 acgagttgcg caaactcggt gcaaaagtcg ccgaagaagc cgaagcaatt cacatcaccc
     2041 cgcccgaaac gccgacaccc gacgccgtca tcgacactta cgacgaccac cgcatggcaa
     2101 tgtgtttctc gctggtttcg ctgttggacg tacccgtcgt catcaacgat ccgaaatgca
     2161 cccacaaaac cttcccgact tattttgaag tgttctcatc gctgaccgaa acagcggaat
     2221 aaggcggcat tttgccgtga ttccggcgca gcgcggcggg cggctcattc tgtaaaaaaa
     2281 gtatgtgcgc cgaggtagtt tttggcgtaa aacggtgtgg agagtttttc ggttttgatg
     2341 gttttgccgc tgctgggggc atggatgaat tcgccgttgc cgatatagag tccgacgtgt
     2401 gagtagcggt gtgtgccgcc ggtgttgaag aatacgaggt cgccggcctt aaggcggctg
     2461 tcggggattt tgcggcttgc cgccgccatg tcgcgggcgg tgcgcggcag cttgacgttg
     2521 agggcgtttt tgtaaacgaa ttgaatcatg ccgctgcaat cgaagccggt tgcggtgctg
     2581 ctgccgcccc atttgtaggg cgtgccgatg agtccgaggc tgtggagcat gagttcctgc
     2641 gagccttgtg tgcggtcgat gtggctgatg cggacggctt ggatttgccg gactgtctgt
     2701 ttgggtttcg gttggcggtg tttgccggag gtcgtgccgc atgaggcgag gagcagtgcg
     2761 ctgagacaga ggaaaagggt tttgtcgggg ggaaacatgg tttttccttt gcgggttcgg
     2821 atatccgtct gaaggcgttt caga
//
LOCUS       DAXHTR010000182         2725 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.182, whole genome shotgun sequence.
ACCESSION   DAXHTR010000182 DAXHTR010000000
VERSION     DAXHTR010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.182"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(37..699)
                     /locus_tag="ACFNML_001912"
     CDS             complement(37..699)
                     /locus_tag="ACFNML_001912"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HFC6393227.1"
                     /translation="MIQAVLFDLDGTLADTALDLGGALNTLLARHGLPPKSMDEIRNQ
                     ASHGAAGLLKLGAGITPDHTDYARWRTEYLDEYDSRYAQDTTLFGGVNELIAELDRRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYIAPEDDTGSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(760..1725)
                     /locus_tag="ACFNML_001913"
     CDS             complement(760..1725)
                     /locus_tag="ACFNML_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003768726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HFC6393228.1"
                     /translation="MSHHHEHHHHDHSHAHTHTANKKVLRVSFIIIAAFMLLEAFGGW
                     LTHSLALLSDAGHMFSDAFSLGVALWAFKLGEKETTLQKTFGYKRFEILTAMFNGLSL
                     VVIAVLIFYEAVKRLLYPPEIATLGMLVISVVGLLVNIGVAVYMLKNSDTEANVNMRG
                     AYLHVLSDLVGSVGAIAAAVLMMAFGWQWADPLASVFVAALVGRSGWSLLKQTLHILM
                     EGAPENIHTDDLLAVIRNTDGVKSVHDLHVWTITSNINVLSCHIVVDGSMTVAESEQI
                     AYRIEHELSHKNIGHCTIQIESERHPHPDSVLCANETDGAHHHHH"
     gene            complement(1771..2598)
                     /locus_tag="ACFNML_001914"
     CDS             complement(1771..2598)
                     /locus_tag="ACFNML_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFC6393229.1"
                     /translation="MKLDIYALLERALLSGDPDEKGRLTDEAFAAVQNMDGAETNTPP
                     LDFPRAGRPDKPVLVAPSQLTPRKMNTAEGYAAMLHAIAHIEFNAVNLALDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRERLRAFGFDYGDFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSETCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPVALFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQSKKVV"
BASE COUNT          543 a          691 c          846 g          645 t
ORIGIN      
        1 tgttaatcca ctatatgttt gtggggcgga tatttttcag ggctgtattt tgtccagaca
       61 ttcgagcaga tcgagtggcg tgcggatgtg gaaatccgcc tgccatgagc cggtatcgtc
      121 ttcgggagcg atgtagcccc attcggcgag gacggttttc atgccggcgt tgcggcctgc
      181 ttggatgtcg cgttcggcat caccgacgta gagtgtgtgt tgcgggtcgg cgtggatttg
      241 tccgcacgca tacagcatgg gtttgatgct gggcttgggt tcgccgcagg tgtcgccgct
      301 gacgacgacg gcgggtggga tgatgaagcc gagtttgggg acgagtttgt cggtgaagcg
      361 cataggtttg ttggtgatga tgccccattt gatgccgcgt ctgtcgagtt cggcgatgag
      421 ttcgtttacg ccgccgaaga gggtggtgtc ttgggcgtag cggctgtcgt attcgtcaag
      481 gtattcggtg cgccatcggg catagtcggt gtggtcgggg gtgatgcctg cgccgagttt
      541 gagcagacct gccgcgccgt ggctggcttg gttgcggatt tcgtccatgc ttttgggcgg
      601 cagtccgtgg cgggcgagca gggtattgag tgcgccgccg aggtctaggg cggtgtcggc
      661 gagcgtgcca tcgaggtcga acaatacggc ttgtatcatg tgttttcctt tggtttttcg
      721 atggggtcgt ctgaaaaggg tttcagacga cctctgggtt tagtggtggt gatggtgtgc
      781 gccgtcggtt tcgttggcac ataagacgct gtcgggatgc gggtggcgtt cgctttcgat
      841 ttggatggtg cagtgtccga tgtttttgtg ggacaactcg tgttcgatgc ggtaggcgat
      901 ttgttcggat tcggcaacgg tcatgctgcc gtccacgacg atgtggcagg ataatacgtt
      961 gatattgctg gtaatcgtcc aaacgtgtaa gtcgtggacg gatttgacgc cgtcggtgtt
     1021 gcggataacg gcgagcaggt cgtcggtgtg gatgttttcg ggtgcgcctt ccatcaggat
     1081 gtgcagcgtt tgtttgagca ggctccagcc gctgcgtccg accaacgcgg caacgaacac
     1141 gctggcgagc gggtccgccc attgccagcc gaacgccatc atgagtacgg cggcggcaat
     1201 cgcgccgacc gagccgacaa ggtcgcttaa aacgtgcaga tacgcgccgc gcatgttgac
     1261 gttggcttcg gtgtcgctgt ttttcagcat ataaaccgcc acgccaatgt tgaccagcag
     1321 cccgacgacg ctaatcacga gcatgcccag cgtagcgatt tcgggcggat aaagcaggcg
     1381 tttgacggct tcgtagaaaa tcagcacggc gatgaccacc agcgacaagc cgttgaacat
     1441 ggcggtgagg atttcgaaac gtttgtagcc gaaggtcttt tgcagcgtgg tttctttttc
     1501 gccgagtttg aacgcccaca acgccacgcc caaggaaaat gcgtcgctga acatatgccc
     1561 cgcgtcggaa agcagcgcga gcgagtgggt cagccagccg ccgaacgctt ccagcagcat
     1621 aaaggcggcg atgatgatga acgacacgcg caatactttt ttgttggcgg tatgcgtgtg
     1681 ggcgtgggaa tgatcgtgat gatggtgttc gtggtggtga gacataatgt tcctcaacag
     1741 aggtcgtctg aaaatccgga ttcggggttt tcagacgacc tttttgcttt gtttcaaacc
     1801 ctgctcgaaa tcttccaaca tatccaattc gaagcggctg aagcccgctt tttcgcgcgc
     1861 ttcgatgttc acatagccgc ggaagataaa catatcgtaa cgggcaatca gactgcggaa
     1921 cagggcgaca ggttccaaac cgcgttcgcg gcagaggtgt tgataccaat ggttgccgat
     1981 ggcgacatga cccacttcgt cgcggtaaat gatgtccaat acgccgcagg tttccgaatc
     2041 gccgcgctgc gccaccttcg cgcgtatccc cggcgtaacg tccagcccgc gcgcttccaa
     2101 cacgcgcggc actaaagcca tacgcaacaa aggatcgtag gcggttttgt atgccatatc
     2161 ccataaatga ttgtgcgcct cgaaatcgcc gtaatcgaag ccgaaagcgc gcagcctttc
     2221 gcgcatcagg cggaagtggt acacctcttc cttcgccact ttcacccagt cgcggacaaa
     2281 ctgaaacggc agcgtgcgga aacggtatgc cgcgtccaaa gccagattga cggcgttgaa
     2341 ttcgatatgc gcaatcgcgt gcagcatcgc cgcatagcct tctgccgtgt tcattttgcg
     2401 cggggtcagt tgcgacggcg cgaccaaaac aggcttgtcc ggccgccctg cgcgggggaa
     2461 atccagcggc ggcgtgtttg tttcagcacc gtccatgttc tgaacggcgg caaacgcctc
     2521 atccgtcagc cgtccttttt catctgggtc gcccgaaagc agggcgcgtt ccagcaaagc
     2581 ataaatatca agtttcatct caagtccgcc gtgttcggaa aacaaatatt atagcgtttt
     2641 aaaaaaacaa gatgaggcat ataatctccg cgattcggca ttccgcgccc aaaccgtcaa
     2701 atatagtgga ttaacaaaaa ccagt
//
LOCUS       DAXHTR010000183         2635 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.183, whole genome shotgun sequence.
ACCESSION   DAXHTR010000183 DAXHTR010000000
VERSION     DAXHTR010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.183"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..910)
                     /locus_tag="ACFNML_001915"
     CDS             complement(<1..910)
                     /locus_tag="ACFNML_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HFC6393230.1"
                     /translation="MLKTPPTLAAELSGKTGVSISAPYANENSRILLSTTDISSENGK
                     IKIQSYGDQYYYARQSELYTFERRSYKTGKWYNRKHITEVKEHKNAKPDAVNLSASQG
                     IDIKSGGSIDAYATAFDAPKGSINIEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYS
                     KAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARAD
                     AKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYI
                     VDIPKGNLKTEIEKLAKQPEYAYLKQL"
     gene            complement(1055..1486)
                     /locus_tag="ACFNML_001916"
     CDS             complement(1055..1486)
                     /locus_tag="ACFNML_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393231.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(1502..>2635)
                     /locus_tag="ACFNML_001917"
     CDS             complement(1502..>2635)
                     /locus_tag="ACFNML_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6393232.1"
                     /translation="EIVGEALTNGKNPDTLTAKEREQILAYSKLVAGTVSGVVGGDVN
                     AAANAAEVAVKNNQLSDKEGREFDNEMTACAKQNNPQLCRKNTVKKYQNVADKRLAAS
                     IAICTDISRSTECRTIRKQHLIDSRSLHSSWEAGLIGKDDEWYKLFSKSYTQADLALQ
                     SYHLNTAAKSWLQSGNTKPLSEWMSDQGYTLISGVNPRFIPIPRGFVKQNTPITNVKY
                     PEGISFDTNLKRHLANADGFSQKQGIKGAHNRTNFMAELNSRGGRVKSETQTDIEGIT
                     RIKYEIPTLDRTGKPDGGFKEISSIKTVYNPKKFSDDKILQMAQNAASQGYSKASKIA
                     QNERTKSISERKNVIQFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          629 a          517 c          563 g          926 t
ORIGIN      
        1 gaagctgttt caggtaggcg tattcgggct gtttggccag cttttcgatt tcggttttca
       61 gattgccttt gggaatatcg acaatgtaac cgccgggtgc ggacagtacg ggcgcaacgg
      121 gaccggtgaa actcggcaat tgcaaggttt cgatgttact gccccgtcct gcctgttttt
      181 gccatagagc agatttgctg ctgctcacgg tttcgttgtg gatcgtggtt ttgatgcctt
      241 cgaggataat cttggcatct gcccgtgcct gctcacctac gcctgcgcgt atggttgcgc
      301 cacccagtgt ggtttcaaac tgtgtgcctt gcagtttggt atcccagccc gattggaggt
      361 tggctgattc tgcaactacc cttgagggca gcgcggtttt catgacttgg gtggtggtgt
      421 cgtgtgcttt gctgtagctg atgccgagaa atctgcgcct tttttggctg tctagtttgt
      481 cgtagttgag ctcttctacg gcatagagtg tcaatttccg cccggcttcg atgttaatgc
      541 tgcctttggg ggcatcgaat gcggtggcgt aggcgtcgat gctgccacca gatttgatgt
      601 cgatgccttg ggatgcgctg aggtttactg cgtcgggctt ggcgtttttg tgttctttga
      661 cttcggtaat gtgtttgcgg ttgtaccatt tgccagtttt gtagctgcgg cgttcaaagg
      721 tatagagttc gctctgtctc gcatagtaat attggtcacc gtaagattga attttgattt
      781 tgccgttttc cgaactgata tccgtggtgc tgagcaggat gcgactgttc tcattggcat
      841 acggtgcgct aatgctcaca ccggttttac ccgaaagttc tgcagcaagc gtagggggtg
      901 tcttcagcac gcacgcgtct gggcagtata tagggaatct gaatatttac ttgcataaca
      961 aatgccgtct gaaaaattgt gagcttttca gacggcattg agccgtaaat catggaacgc
     1021 gtgcgtgctg aagcacacac cttacgcatg gattttaggt ttcatgcagg ctacagtttg
     1081 tttgagaata tgttttttgt atttctttac actttttttg atattcaggg tgagctatta
     1141 agaaatcaga tattgcaata ttaaagtaat gatatgctaa attaataggt ataattaatt
     1201 cttcaggatt gaaatcatct ccaactgaaa aataaataga aggagcttta tcaatatact
     1261 caaatccatt tacctcttca taatctccaa tccatctggt agcatctatt ttttcatatt
     1321 gagcacctat tactccaaaa tgtccggtag ttgtaatatt tttaaaacac tctaaaagcg
     1381 tatctttagt ttcaaattgg taaattgttt caaaaaaaat cgaaataaca aaatggttgt
     1441 tatttataga ttttttattt aagtttagaa aaatattatt tttcataatt tttcctttaa
     1501 attattctgg gtgaatgttt gtaattcttc ctgtatttac atcaaaatat gatctaaatt
     1561 tgattccgtc aaaggtttct gagaattgaa tgacattttt tctttccgat attgatttag
     1621 ttctttcatt ttgagcaatt ttagaggctt ttgaatatcc ttgtgaagca gcattttgag
     1681 ccatttgaag tattttatca tcagaaaatt ttttaggatt ataaacagtt tttatacttg
     1741 aaatttcctt aaatccacca tcaggtttac ctgtcctgtc tagtgtagga atctcatatt
     1801 taattcgggt aatgccttca atatcagttt gggtttcaga ttttacgcgt cctcctcgtg
     1861 aatttagttc tgccataaaa ttggtgcggt tatgggctcc tttaatgccc tgtttttgac
     1921 taaaaccatc agcatttgcc agatgtcttt ttaggtttgt atcgaaactg atgccttccg
     1981 ggtatttgac attagtaata ggtgtatttt gttttacaaa ccctcttggt attggaatga
     2041 atctaggatt aactcctgaa ataagtgtat aaccttggtc ggacatccat tcggataaag
     2101 gctttgtatt gcccgattga agccaagatt tagcagcagt attcaaatga taagactgta
     2161 aagccaaatc tgcttgggtg taagatttgc tgaataattt ataccattca tcatctttac
     2221 caattagacc tgcttcccaa gatgaatgaa ggcttctact atcgatcaaa tgttgttttc
     2281 tgattgttct acattcagta ctacgggata tatccgtaca tattgcaatc gaagcagcaa
     2341 gtcttttatc agcaacattt tgatactttt ttacagtatt ttttctgcac agttgaggat
     2401 tattctgttt ggcgcatgca gtcatttcgt tatcaaattc tctaccctct ttgtcgctaa
     2461 gctgattatt tttcaccgct acctcagccg cattcgccgc cgcatttaca tcgccgccga
     2521 ccacaccgct taccgtaccg gcaaccagtt tgctgtatgc caaaatctgt tcgcgttctt
     2581 tagctgtcaa agtgtcagga tttttgccgt ttgtcaaagc ctccccgact atctc
//
LOCUS       DAXHTR010000184         2635 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.184, whole genome shotgun sequence.
ACCESSION   DAXHTR010000184 DAXHTR010000000
VERSION     DAXHTR010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.184"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            3..512
                     /locus_tag="ACFNML_001918"
     CDS             3..512
                     /locus_tag="ACFNML_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HFC6393233.1"
                     /translation="MPEIIRDLLEMPSETAIADITRTYSAHYLNPNNRNMSLFPQALP
                     CLDKLKAQGYWLAVATGKGRAGLDNAISQTATGGYWLATACAGEYPSKPSPEMVFGIC
                     GKLGLDPKEALVVGDTAHDLHMAANAGAAAVGVATGAHSREQLRTAPHLAVLDGLSEL
                     PGFLAQHYA"
     gene            complement(599..1567)
                     /gene="ispH"
                     /locus_tag="ACFNML_001919"
                     /gene_synonym="lytB"
     CDS             complement(599..1567)
                     /gene="ispH"
                     /locus_tag="ACFNML_001919"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFC6393234.1"
                     /translation="MNEKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLVYSAHGVSKAVRQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNAGYLQRAWFEGKNKVGVTAGASAPEVLV
                     REVLATIRGWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(1596..2093)
                     /gene="lspA"
                     /locus_tag="ACFNML_001920"
     CDS             complement(1596..2093)
                     /gene="lspA"
                     /locus_tag="ACFNML_001920"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFC6393235.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKT
                     QEEKY"
BASE COUNT          608 a          809 c          642 g          576 t
ORIGIN      
        1 gcctgcccga aatcatccgc gacctgctcg aaatgccgtc tgaaaccgcc attgccgaca
       61 tcacacgcac ttattccgca cattacctca atcccaacaa ccgcaatatg tccttatttc
      121 ctcaggcact gccctgtctg gacaagctca aagcacaagg atactggctt gccgtcgcca
      181 cgggcaaagg gcgggcgggt ttggacaacg ccatcagcca aaccgccacc ggcggctatt
      241 ggctcgccac cgcctgcgcg ggggaatatc cctccaaacc ctcgcccgaa atggtattcg
      301 gaatctgcgg caaactggga cttgacccga aagaggcatt ggtcgtcggc gatacggcgc
      361 acgacctgca tatggcggca aacgcaggcg cggcggcagt cggcgtggcc accggcgcac
      421 attcgcgcga acagctccgc accgcaccgc atctcgccgt attggacggt ttgtccgaac
      481 tgcccggttt tcttgcacag cattacgcct gattggtttc cgcatcagac actcaaaaca
      541 aatgccgtct gaagcctgtt cagacggcat ttgtgttgcc caaacattca acgcttgctc
      601 aacgtttgca caaatcgggt ttggtttcgc cctcgcggcg cagctctttg ggcaggacga
      661 acacaatgct ttcttccgca ccctcgcctt cgcgtacggt ttcgtgcccc catccgcgta
      721 tggttgccag tacttcccgc accaacactt cgggcgcgga cgcgcctgcc gttacgccga
      781 ctttgttctt gccctcaaac cacgcgcgtt gcaggtagcc tgcattgtcc accatatacg
      841 catcggttcc gatgcctgcc gccacttcgc gcaggcggtt gctgttggac gaattgggcg
      901 aaccgaccac aatcacgatg tcgcactgtt ctgccaactc tttgacggcg gtttgccggt
      961 tggtcgtcgc atagcagata tcttccttgt gcggattgcg gatattgggg aaacgcgcgt
     1021 tcagcgcggc gatgatgtct ttggtttcat cgaccgagag cgtggtttgg ctgacatagg
     1081 cgagtttgtc ggggtttctg acttcgagtt ttgccacatc tccgaccgtt tcgaccaaaa
     1141 gcattttgcc cggcgcaagc tgccccatcg ttccttcaac ctcgacgtgc cccttatgcc
     1201 cgatcatgat gatttcacag tcttgggcat ccagtcgggc gacttcctta tgcactttcg
     1261 tcaccagcgg gcaagtcgca tcaaatacgc ggaaaccgcg ctccgccgct tcttgccgca
     1321 ccgccttcga tacgccgtgt gccgaataaa ccagtgtcgc gcccggcggc acttccgcca
     1381 agtcttcaat aaacaccgca cctttttcac gcaggttgtc cacgacgaat ttgttgtgaa
     1441 cgacttcgtg gcgcacataa atcggcgcgc cgaattcttc caaagcacgt tcgacaatac
     1501 tgattgcccg atccacgccg gcgcagaagc cgcgcggatt ggcaagaatg atggttttct
     1561 cgttcataag cccggtattt cgttttcaga cggcatcaat atttttcttc ttgggtttta
     1621 cggtggacga tgttgtccaa cactgccaac accgcaccga cgcagataaa gctgtcggca
     1681 atattaaagg cgggataaaa ccaattttgc caataaaaca ataagaaatc gacgacatga
     1741 ccgtgtatca ggcggtcgat gacattgcct aacgcaccgc cgataatcat cgccgcaccc
     1801 gttttgccga gggttgcaaa ctcatcgcgc aagatggcgc gtaccaaata cgcgctcacc
     1861 gccaccgcca gcaccaaaaa aaagtatttt tgccagccgc cctgatcggc aaggaagctg
     1921 aacgccgcac ccgggttgta caccaaggtc aaatcgaaaa aggaaggaat gacattgacg
     1981 cgttcccgat actgaaacga cgacagcacc gcccacttcg accactggtc cagcacgatg
     2041 gcggcaagtg ccaataccca atagcgcgtt ttacttgaaa cagatgaaga catatttttc
     2101 aacagccggt aaaagagtac cattttaccc gaaaaccccc tgcctgtacc cgaaacggca
     2161 aatgccgtaa tcttgaaacc cgtcattccc gcgcaggcgg gaatctagac ctgtccgcac
     2221 agaaacttat cggataaaaa cagttgctca aaccccgcgt tctagattcc cactttcgtg
     2281 ggaatgacgg ttcagttgcg ttccaacaac accgcaatct cgaaatccgt cattcccgcg
     2341 caggcgggaa tccagacctc cgacgcggcg ggaatctatc ggaaatgact gaaaccccga
     2401 gattctagat tcccactttc gtgggaatga cggttcagtt gcgttccgac aacaccgcaa
     2461 tctcgaaatc cgtcattccc gcacaggcgg gaatccagac ccacgacgcg gcgggaatct
     2521 atcggaaatg actgaaacct cgagattcta gattcccact ttcgtgggaa tggcggttca
     2581 gttgcattcc gacaacaccg taatcttgaa acccgtccga caacaccgta atctt
//
LOCUS       DAXHTR010000185         2611 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.185, whole genome shotgun sequence.
ACCESSION   DAXHTR010000185 DAXHTR010000000
VERSION     DAXHTR010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.185"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            1..300
                     /locus_tag="ACFNML_001921"
                     /pseudo
     CDS             1..300
                     /locus_tag="ACFNML_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(347..1756)
                     /gene="dacB"
                     /locus_tag="ACFNML_001922"
     CDS             complement(347..1756)
                     /gene="dacB"
                     /locus_tag="ACFNML_001922"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HFC6393236.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGDGKLPAVSEQAASAVRRELAV
                     SGIDIADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1921..2487
                     /locus_tag="ACFNML_001923"
     CDS             1921..2487
                     /locus_tag="ACFNML_001923"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFC6393237.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTAFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          619 a          611 c          728 g          653 t
ORIGIN      
        1 gtttatacgg attgttatcg tagctatgat gtattagatg tgagcgaatt tagccatttt
       61 agcttcgctg aaacttcgtt ttcgtatcaa tcacagcaca cattttgctg aacgacaaaa
      121 ccatattaat ggaattgaga acttttggaa ccaggcaaaa cgtcatttac gcaagtttaa
      181 cggcattccc aaagcgcatt ttgagctgta tttaaaggag tgcgaatggc gttttaacaa
      241 cagtgagata aaagttcttg ttccatttta aaacaattag taaaatcgag tttgtcctag
      301 ttatctagga cagcccctta aatttaatcc actatatttt cctgtttcag gctcggcgtt
      361 ctttgcacat cagtttcgca tccagccagc catcgccgcc ggagatgatg ttgttggcaa
      421 cgaagttgtc caagtcgggc agcagggaaa cggcgcggcc gctgttgatg atgacgacca
      481 ccgccatcgg tttgtcgccc agccaataac ctgcaagggc gcggacattg ttgagcgtgc
      541 cggtttttaa gcgcaacagc ccgccgcttt gtttgaagcg gttgcgtaaa gtcccgtctg
      601 tgccggcgat gggtagcgta tcgatgaaat cttgtgcaaa cgggctgaaa taagccgttt
      661 ccaacatttg cgccatcatt ctcgccgtta ccctttcttt tctggacagg cctgaaccgt
      721 tttccaaaac caaatccgca atatcgatgc ccgacacggc aagttcgcgc cggacggcag
      781 atgccgcctg ttcggaaacg gcgggcagtt tgccgtcgcc gccgagtttg aggaagacgg
      841 aacgcgcaat tagattgtcc gaacgcttgt tcatgtccgt caaaatttcc ttcatcggct
      901 ttgagtgtgc aacggcaagc gtctgcgcgc cttccggcgt gtcggatatg ccgatgccgt
      961 ctgaaatccg tccgccgccg agcagccagt ggttggtaaa actttgccgg atcagttcgt
     1021 caagcgcgaa catccggaca ccgacaggct tgcccaaaca gctctcggga atattgccgc
     1081 gcaatttcag cgtattgtcc gaaaaagatg cacgcatcag ttttttgatc gaagggcagg
     1141 cagcttggga ggcggtaatt ttcaagttgt tttgggcgaa aatatgcggc aaaggcggat
     1201 cggtgaggat gtcggtactg tcggcggcat tgcgttcggc gcgcaccata accataccgg
     1261 cagacagcat agttggattg gggggcgtca taaacggcga accgctgtcg gcttcgaaat
     1321 cgtcggggct gccgacttcg ccccacaggc tgtggtcgag catcaggtgt cccgtgatat
     1381 tgcgtatgcc ttgttcgcgc aactggcgtt ggacggcaag caggttttcc tgattgaaaa
     1441 cggggtcgcc gctgcccgcc caatacaggt ttccgtcaag cgtgccgtcg tttaccgtac
     1501 cgttgctttt aaactcggtc gcccagcggt aattgctgcc gaaggttttg aaggcggcaa
     1561 acgcggtaac gagtttcatt gtggaggcgg ggttgacggg gacatccgag cggtggtcaa
     1621 tgatgacttt tccgctgtca agctcttgga catatacggc gatttcgttt tgcggaatgc
     1681 ggccggtatc gagcgcgtgt gcggcgaggg aggcgagaag cagcagcagg gaggccgctg
     1741 ttttggggaa attcataggt aaatctgtta cataaaaaaa gcaattataa ggcaaagccg
     1801 gataagtggg aacgctgtgg cggcgggccg gcttgtttgc aggggaaatc acatatataa
     1861 taatcgctat cattatgaaa tgattgagac gcacaaactt aatattcagg aggaatgatt
     1921 atggctaaga aaatcagtat tttggtgggc agcctgcgcc gtgcttcttt tgcgcgcaaa
     1981 gtggcattga atgcggcgga gatgttcccc gaaggctggc aggcggaaat cgtcgaaatc
     2041 ggacatctgc cgctttacaa tttcgattat gacgaccctg cggtggaaga tgtgccgctg
     2101 cccgaaagct acacggcttt ccgcgaaacg attaaggctt cggacggcat tttgttcgtt
     2161 acgtccgaaa ataaccgcac cattcccgcc tgtttgaaaa atgcggtgga catcggctcg
     2221 aaaccgaatg ccgacgtggc gtggaaaaac aaaccggccg gtatcatcag ccattccgtc
     2281 ggcaagatgg gcggttacag ttcgcaaaaa aacctgcgcc ttgcgctgtc ttatttcgat
     2341 atgcccgtaa ccggacagcc ggaagtgttt ttgggcaatt cgccgacgct gtttgatgaa
     2401 aacggcaaat tgatcgactc ggcaagggat tttgttcagt catatattaa tcagtttgtt
     2461 ggtttgattg aaagaaatgc caaataaaca aaccaagtcg aaaggccgca aaaccggttc
     2521 gggttttgcg gcttttttat atgggctggg cagcgtcaag gtttgccgtt tgccggataa
     2581 tgcgcggcaa atgaccggcg tgtttatagc g
//
LOCUS       DAXHTR010000186         2575 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.186, whole genome shotgun sequence.
ACCESSION   DAXHTR010000186 DAXHTR010000000
VERSION     DAXHTR010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.186"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(192..263)
                     /locus_tag="ACFNML_001924"
     tRNA            complement(192..263)
                     /locus_tag="ACFNML_001924"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(228..230),aa:Arg,seq:cct)
     gene            complement(283..729)
                     /locus_tag="ACFNML_001925"
     CDS             complement(283..729)
                     /locus_tag="ACFNML_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFC6393238.1"
                     /translation="MNFSFVPLFLVTLILLGVVSNNNSITISATILLLMQQTALVQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(734..1708)
                     /gene="ispB"
                     /locus_tag="ACFNML_001926"
     CDS             complement(734..1708)
                     /gene="ispB"
                     /locus_tag="ACFNML_001926"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFC6393239.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            1932..2240
                     /gene="rplU"
                     /locus_tag="ACFNML_001927"
     CDS             1932..2240
                     /gene="rplU"
                     /locus_tag="ACFNML_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFC6393240.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            2265..2537
                     /gene="rpmA"
                     /locus_tag="ACFNML_001928"
     CDS             2265..2537
                     /gene="rpmA"
                     /locus_tag="ACFNML_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFC6393241.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
BASE COUNT          755 a          699 c          572 g          549 t
ORIGIN      
        1 aaacaacaga tttccaagcc ggaacatctt ttacggagcc tgaaaatcat ttcatgattc
       61 taccgtccta aaggtcggtt tttcaagcag gaagaaaaag ttttcagatg gcaaaaaagc
      121 cctccaacac tgaaaggcct tatatcggaa acttcccgca acacgggaaa cagacaaatg
      181 aaatcgtcaa acctcgccaa caggaatcga acctgtattt tacgcttagg aggcatacgt
      241 tctatccgtt gaactatggc gagccgaaat gaaaaggaga ttttaaccct ttccgacgac
      301 aaaagacaag atgccggccg caatcagcgg cccgacaggg ataccgccca taaatgccac
      361 cccgataacc gtcccgatta acagccctgt aattaaaaca ggctgctgtc ccatcaaagg
      421 cacgccgcgt cccgccagcc aagccacgaa aataccgata aaaacggcgg atatcatttt
      481 aaaattcaaa aattcggcaa cgggaggaac ctgcgccttt cctgaaacca acggactcaa
      541 aacccctatg gtcaaaagaa tgataccgag attcaaccca tgcttctcga ccaacgggac
      601 aaactgtacc aatgccgtct gctgcatcag cagcaatatg gttgccgaga tggtaatcga
      661 attgttgttg ctgaccaccc ccaacagaat cagcgtaacc agaaacaggg gaacaaaact
      721 gaaattcatc gcctcaagac accctgacca aagattcctt cgccagctga atcatggcat
      781 ccttcacttc gctgtcgggc agggcatcca aggcggtaac ggcacaatcg actgctttgc
      841 gcgcctcgcc tatcgaatat gccaaagcat ccgaacggac gacataatcg tggattttct
      901 caaaatagct gcgatctgca ttttccaaag cagtacgcac atcgttcgca acctgttcgg
      961 aaccctgacg catcagataa atcaaaggca aagtcggttt tccttccgcc aaatcgtcgc
     1021 cgacgttttt gccggtttct tcggtttcgc cagaatagtc cagcacatcg tcaataattt
     1081 ggaatgccgt accgacatac ataccgtagt ctttcaacgc ccgttcgtgt tcgggggaag
     1141 ccttgcccaa aattgcgccg acctgcgccg ccgcttcaaa caattttgcc gttttatatt
     1201 ggatgacttg gatatattgt tcttcggtaa tgtccgtatt gccgatgttc atcagctgca
     1261 tgacttcgcc ctcggcaata atgttggttg catccgccat cacttccaaa acgcgcatac
     1321 tgcccgaggc aaccatcagt tgaaaggcgc gcgtgtataa aaagtcgcca accaacacag
     1381 ccgccgcatt gccgaacaga ttgtttgccg ttgcccgccc acggcgcaaa tcgctttcat
     1441 cgacgacatc gtcgtgcaga agcgtcgaag tgtggataaa ctcgaccatc gccgccagcg
     1501 aatacagttt ctcgtcatca taaccgaccg ccttacccgc caaaatcgtc ataatcggac
     1561 gcaggcgttt gccgcccgcg ctgatgatat atgtaccgat ttgcgaaatc agtgcgacat
     1621 cggattgcac cgcacggttg atgacttcat tgactttggc aaggtcttca ggcagatgtc
     1681 gctggaaata gggcagattc tcgagcatgg cagactctca ggtttacgtt tccgtcaaac
     1741 ggcagatacc gcttgaaagc tgattcaaat agcgggcaat tatatcagca aagccatcag
     1801 ggtacatctt ttcagacggc atatggttat ccgaaccgcg caaactttga caaaacaggc
     1861 aaataacaat acaatgccga gttccgcaca atggtgtgcg gatattaacc ataaccctta
     1921 tggagttgag tatgtacgcg gtcgtaaaaa ctggcggcaa acagtataaa gtttccgtcg
     1981 gcgaaaaatt gaaagtagaa cagataccag ccgaactcga cagccaaatc gaactgaccg
     2041 aagttttgat gattgctgac ggcgaatctg taaaagttgg cgcacccttt atcgaaggtg
     2101 caaaagtaac ggctaaagta gtggcacacg gtcgtggcga aaaagtccgc atcttcaaaa
     2161 tgcgccgccg caaacactac caaaaacgcc aaggccaccg ccaaaatttc acccaaatcg
     2221 aaatcgtggc aatcgcctaa ccttaaaact taggagcatt acaaatggca agtaaaaaag
     2281 caggcggcag cacccgcaac ggtcgcgatt cagaagccaa acgcttgggc gttaaagcct
     2341 acggcaacga gctgattccc gcaggttcca tcatcgtccg ccaacgcggc accaaattcc
     2401 acgcaggcga caacgtaggt atgggcaaag accacacttt gttcgccaaa gttgacggtt
     2461 atgtcgaatt caaaaccaaa ggcgcgctga accgcaaaac cgtaagcatc cgtccttaca
     2521 ccggttctga agaataattc aaccgaacag gaagccgcat cttttcacga tgcgg
//
LOCUS       DAXHTR010000187         2557 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.187, whole genome shotgun sequence.
ACCESSION   DAXHTR010000187 DAXHTR010000000
VERSION     DAXHTR010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.187"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            108..671
                     /gene="gmhB"
                     /locus_tag="ACFNML_001929"
     CDS             108..671
                     /gene="gmhB"
                     /locus_tag="ACFNML_001929"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFC6393242.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGC
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTDADNCNCRKP
                     KPGMIEDIIGRFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENTAPQAD"
     gene            700..1443
                     /locus_tag="ACFNML_001930"
     CDS             700..1443
                     /locus_tag="ACFNML_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFC6393243.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLVRKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPYASGSEAELM
                     GKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            1440..2132
                     /locus_tag="ACFNML_001931"
     CDS             1440..2132
                     /locus_tag="ACFNML_001931"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFC6393244.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHSRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CRKTTGIRLNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
BASE COUNT          611 a          853 c          614 g          479 t
ORIGIN      
        1 accgtactgg tttttgttaa tccactatat cacgcggcac aggcgcatca ctgccgatat
       61 ttcagtataa tgccaccccc gacctgcccc aacccaaagg aaacgcgatg aaactcatca
      121 ttctcgaccg cgacggcgtc atcaatcagg accgcgatga cttcgtcaaa tccgttgacg
      181 agtggatacc tgtcgaaggc agcatggatg cggtggcatt tctgacgcag gcaggctgca
      241 ccgtcgccgt tgccaccaac caatccggca tcgggcgcaa atattttacc gttcaaaacc
      301 ttaccgaaat gcacgccaaa atgcaccgcc tcgtccgtca ggcaggcggc gaaatcaacg
      361 gcatctggtt ctgcccgcac accgatgccg acaactgcaa ctgccgcaag cccaaaccgg
      421 gtatgattga agacatcatc ggacgcttca acgcccaagc ctcggaaacc tggctggtcg
      481 gcgacagcct gcgcgatttg caggcaatcg atgccgtcgg cggcaaaccc gcgctggttc
      541 tgaccggaaa aggcaaaaaa acgctctccc aacacggaca cgaattgccc gaacacacac
      601 aggttttcga taccctgctc gatttctcac aatacatcat gcaggaaaac accgcaccgc
      661 aagccgactg aacataccgc attccgacaa ggcaaaacca tgctcatcat ccgcaacctg
      721 atttactggc tgatactctg ttccaccctg attttcctct ttccctttat gctgctcgcc
      781 tcgcctttcc gagacggggc gcacaagatg gcgcgggtct gggtcaaaat cctcaacctc
      841 tcgctcaaac acatcgtcgg gctcaaatac cgcatcatcg gcgcggaaaa catccccgac
      901 cgccccgccg tcatctgcgc caaacaccaa agcggctggg aaacgctcgc ccttcaggac
      961 atttttccgc cgcaggttta cgttgccaaa cgcgagttgt tcaaaatccc ctttttcggc
     1021 tggggcttga aactggtcaa aaccataggc atagaccgca acaaccgccg cgaagccaac
     1081 gagcagctca taaaacaggg gttggtgcgc aaaaacgaag gctattggat taccattttc
     1141 cccgaaggca cgcgccttgc gcccggaaaa cgcggcaaat acaaactcgg cggcgcgcgc
     1201 atggcgaaaa tgtttgagat ggacatcgtc cccgtcgccc tcaacagcgg cgaattttgg
     1261 ccgaaaaact cctttctgaa atatccgggg gaaatcaccg tcgtcatctg tccgaccatc
     1321 ccgtacgcaa gcggcagcga agccgaattg atgggaaaat gcgaacacct catcgaaacg
     1381 cagcagccgc tcatttccgg cgcaggcccg tttgccgcca aaatgccgtc tgaaaccgca
     1441 tgaccgcctt tgtccacacc ctttcagacg gcatggaact gaccgtcgaa atcaagcgcc
     1501 gtgccaagaa aaacctgatt atccgccccg ccggcacaca taccgtccgc atcagcgtcc
     1561 caccctgctt ctccgtctcc gctctaaacc gctggctgta tgaaaacgaa gccgtcctgc
     1621 ggcaaacact ggcgaaaaca ccgccgccgc aaactgccga aaaccggctg cccgaatcca
     1681 tcctcttcca cagcagacag cttgccctca ccgcccatca agacacgcaa atcctgctga
     1741 tgccgtctga aatccgtgtt cccgaaggcg cacccgaaaa acagcttgcg ctgctgcggg
     1801 actttttgga acggcaggcg cacagttacc tgattccccg cctcgaacgc cacgcccgca
     1861 ccacacaact gttccccgcc tcctcctcgc tgacctctgc caaaaccttc tggggcgtgt
     1921 gccgcaaaac cacaggcata cgcctcaact ggcggctggt cggcgcaccg gaatacgttg
     1981 ccgactatgt ctgcatacac gaactctgcc acctcgccca tcccgaccac agccccgcct
     2041 tttgggaact gacccgccgc ttcgccccct acacgcccaa agcgaaacag tggctcaaaa
     2101 tccacggcag ggaacttttc gccttaggct gacgcggacc ggaccgaccc gccgctttca
     2161 gacggcatcc gtgccggaac aggcacgcgc ccgcccgatt caaaccgcga tgacgctttg
     2221 ccgccggttc ggggcaggat ggcggcacac acgccgtctg ccgcgtttca tttcacacca
     2281 ctcttccgaa acccgaaacc cgcccggtcc gacgtgcggt atgaaacgct taagctgacg
     2341 cgaagtcttt tactgatttg cccgcgaaaa tgccgtctga aaggttttcg gacggcattt
     2401 ttttgcgttt cccaggatgg cggcggattc gtaaaaggcg gtcagggtgg attgtaggat
     2461 gggttgagac ctgccgaatc cgccgcatct gccaaatcta ccgccgtcat tcctacgaaa
     2521 gtgggaatct agaacgcggg gttaagaaaa cctgcat
//
LOCUS       DAXHTR010000188         2551 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.188, whole genome shotgun sequence.
ACCESSION   DAXHTR010000188 DAXHTR010000000
VERSION     DAXHTR010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.188"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(3..1379)
                     /gene="mpl"
                     /locus_tag="ACFNML_001932"
     CDS             complement(3..1379)
                     /gene="mpl"
                     /locus_tag="ACFNML_001932"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFC6393245.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            complement(1441..1746)
                     /locus_tag="ACFNML_001933"
     CDS             complement(1441..1746)
                     /locus_tag="ACFNML_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393246.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            complement(1758..2110)
                     /locus_tag="ACFNML_001934"
                     /pseudo
     CDS             complement(1758..2110)
                     /locus_tag="ACFNML_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691158.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2372..>2551)
                     /locus_tag="ACFNML_001935"
     CDS             complement(2372..>2551)
                     /locus_tag="ACFNML_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393247.1"
                     /translation="YYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWKLILLSN
                     TVAAAVLAWVWWKWF"
BASE COUNT          573 a          709 c          600 g          669 t
ORIGIN      
        1 ggctatctca aagcgtccag cagtttggtg tgtattccgc cgaaaccgcc gttgctcatc
       61 accaaaatat ggtcgccggt tcgggcgttt ttcacaattt cggcaacgaa ggcatcgaaa
      121 tccttgccga cgtgcagcct gccgcccaaa ggtgcgaggg cttcggcgac gttccagtcc
      181 acaccgccgg cgtagcagaa cacttggtcg gcttctttga ggctttcggg cagggcggac
      241 ttcatcgtgc ccagcttcat cgtgttggaa cgcggctcga ggacggcgag gatgcgggcg
      301 tttccgacgc gctgacgcag accttctatg gtggtttcga tggcggtcgg atggtgggcg
      361 aagtcgtcgt aaacagtgat gccgtttacc gtgcctttga tttccatgcg gcgtttgacg
      421 tttttaaacg tgctcaaggc ttcgcaggcc gtctgaatgt cgactccggc atgacgcgcg
      481 gcggcgatga cggcgagcgc gttcatgcgg ttgtgtccgc ccatcaaact ccaagcgacg
      541 tgtccggctt ttttgccgtc aagcaacacg tcgaacgagc catcggcatt ggcttcgccg
      601 gcctgccagc cgtgttccgt gccgaatttt tccaccggcg tccagcagcc tttgtccaaa
      661 gtgtcttgca ggctttgctg ccgtccgttg cagacgatga ggccttcaga cggcacggta
      721 cgcacgaggt ggtggaactg ggtctgtatc gcgcccaaat cggcgaagat gtcggcgtgg
      781 tcgaattcca gattgttcaa cacggcggta cgcggacggt aatgcacgaa tttggagcgt
      841 ttgtcgaaaa acgcggtgtc gtattcgtcg gcttcaatga cgaaaaacgg cgattggctg
      901 ttcgggtctt ggcgcggcgt ttgcggcagg cgggcggaaa cgctgaagtt ttccggtacg
      961 ccgccgataa ggaagcccgg tgcgagtccg gcatattcca agacccatgc gagcatggac
     1021 gcggtggtcg ttttgccgtg cgtccccgcc acaccgagta cccaatgatg gtgcagcacg
     1081 ttttcagcca gccattgcgg accggaaata taaggcagcc cgcggttcaa aatcgcctca
     1141 accacatcca tcccgcgctt ggcgacattg ccgatgacgt aaacgtcggc tttaaattcc
     1201 tccaactgcg ccgcgtcgaa gccttcgtgt acgcctatgc ccaaggcttc gagctgggtg
     1261 ctcatcggcg gatacatctt cgcatcgcaa ccgctgactt caaaccctgc ttctttggca
     1321 atggcggcaa tcccacccat aaacgtgccg ccgataccga taatgtggat gtgtttcatc
     1381 agagttccct aatcgttcaa attctattat tcaacgccgt catggctgat tcggtttcaa
     1441 tcaatgcgtc tttgcaccgg tattcgaagc atccttatgc gtattctctc tcggattttg
     1501 gacgtgttcg tccctcaccg tgtccgaata atcgccgacc cctgcgtctt ccgccctgtc
     1561 ttttactgcc ggtctaaccg aactgtacac ccgatcccgc cccaaacccg cgttggtaca
     1621 atgttttccc ggagtttggc tgtaactggt tttaccatta tggcggcacg cgtaaacatt
     1681 tgccgaaaat acggtctgaa ccgctgtaac aagcaacata cttccaatta ttttagatac
     1741 gcgcatatca acctccttta tcgttcttct tcaaaaaagg gaatgtattt caatagcact
     1801 tcatctaatt ctttcattcc ctgctcataa ttgaacgggt attcccaacc atcccctact
     1861 ttgatttcag aaatatcttt aaacttccgc ttatgggcgg acactttgtc catatcgtca
     1921 tcaatttcct gtttgccgaa cggtattttc cgcttttcca aaaccttgtc ataataacaa
     1981 gataaattaa gcaatagccc ccaagcacta tttgcaatta cgacgaatct atgtttttct
     2041 ctggttaaat aatctgcacc gctatcatag attgcccttg tcgggtcata aaaaattcgc
     2101 aatttttcat agcgtcccca cactttaacc gattcgcaat tttatatccg cgattttgca
     2161 agctgcccat aaaaaccgat tgcctgccaa gacggcacta cttgttaaag atgcccgata
     2221 atgccgcaac tcttccgtat ttaccgacaa acgccctatt tgcttagagc tgttttgttt
     2281 tccacaaaac tttaattcgt tgatttaaca aattttttac aaaattcttt atttgttcgg
     2341 attccattag cacgattttg agcaatatct gtcaaaacca tttccaccac acccaagcca
     2401 aaaccgccgc cgccacggta ttggacaata agatgagttt ccactgcccg tccttccgca
     2461 atgccgcctt cttgggcttc aaagcacccg cataaaaaaa cggcgacagc agcatagaag
     2521 cgatacataa gaaaatcaat acgccgtaat a
//
LOCUS       DAXHTR010000189         2536 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.189, whole genome shotgun sequence.
ACCESSION   DAXHTR010000189 DAXHTR010000000
VERSION     DAXHTR010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.189"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..554
                     /locus_tag="ACFNML_001936"
     CDS             <1..554
                     /locus_tag="ACFNML_001936"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393248.1"
                     /translation="SIEKSQESIRKNRNPHRVIPAQAGIQKRNATGIYRKKQKPPPRH
                     SRAGGNPETQRGRTLSEKTETLTTVIPAQAGIQKRNATGIYQKKQKPHRPSFPRRRES
                     RNTTRQDFIGKNRNPTDRHSRKSGNPATEKPQESISKNRNPTAVIPAKAGIQTRRHGN
                     LPNKTVSLDSTSQIPAFAGMTRF"
     gene            666..2468
                     /locus_tag="ACFNML_001937"
     CDS             666..2468
                     /locus_tag="ACFNML_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ESPR-type extended signal peptide-containing
                     protein"
                     /protein_id="HFC6393249.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVATAVLATLLS
                     ATVQANATDEEDDEELEPVVRTAPVLSFHSDKEGTGEKEEVGASSNLTVYFDKKGVLK
                     AGTITLKAGDNLKIKQNTNENTNENTNASSFTYSLKKDLTDLTSVGTEKLSFSANGNK
                     VNITSDTKGLNFAKETAGTNGDTTVHLNGIGSTLTDTLLNTGATTNVTNDNVTDDEKK
                     RAASVKDVLNAGWNIKGVKPGTTASDNVDFVRTYDTVEFLSADTKTTTVNVESKDNGK
                     KTEVKIGAKTSVIKEKDGKLVTGKDKGENGSSTDEGEGLVTAKEVIDAVNKAGWRMKT
                     TTANGQTGQADKFETVTSGTKVTFASGNGTTATVSKDDQGNITVKYDVNVGDALNVNQ
                     LQNSGWNLDSKAVAGSSGKVISGNVSPSKGKMDETININAGNNIEITRNGKNIDIATS
                     MTPQFSSVSLGAGADAPTLSVDDEGALNVGSKDANKPVRITNVAPGVKEGDVTNVAQL
                     KGVAQNLNNRIDNVDGNARAGIAQAIATAGLAQAYLPGKSMMAIGGGTYRGEAGYAIG
                     YSSISDTGNWVIKGTASGNSRGHFGTSASVGYQW"
BASE COUNT          818 a          645 c          615 g          458 t
ORIGIN      
        1 aatccataga aaaatcacag gaatctatca gaaaaaacag aaacccccac cgcgtcattc
       61 ccgcgcaggc gggaatccag aaacgcaacg caacaggaat ttatcggaaa aaacagaaac
      121 ccccaccgcg tcattcccgc gcaggcggga atccagaaac acaacgcggc aggactttat
      181 cggaaaaaac agaaaccctc accaccgtca ttcccgcgca ggcgggaatc caaaaacgca
      241 acgcaacagg aatttatcag aaaaaacaga aaccccaccg accgtcattc ccgcgcaggc
      301 gggaatccag aaacacaacg cggcaggact ttatcggaaa aaaccgaaac cccaccgacc
      361 gtcattcccg caaaagcggg aatccagcaa ccgaaaaacc acaggaatct atcagcaaaa
      421 acagaaaccc caccgccgtc attcccgcaa aagcgggaat ccagacccgt cggcacggaa
      481 acttaccgaa taaaacagtt tccttagatt ccacgtccca gattcccgcc ttcgcgggaa
      541 tgacgagatt ttaagttggg ggaatttatc agaaaacccc caaccccaaa aaaccgggcg
      601 gatgccgcac catccgcccc caaaccccga tttaaccatt caaacaaacc aaaagaaaaa
      661 acaaaatgaa caaaatatac cgcatcattt ggaatagtgc cctcaatgcc tgggtcgtcg
      721 tatccgagct cacacgcaac cacaccaaac gcgcctccgc aaccgtggcg accgccgtat
      781 tggcgacact gctgtccgca acggttcagg cgaatgctac cgatgaagaa gacgatgaag
      841 agttagaacc cgtagtacgc actgctcccg tgttgagctt ccattccgat aaagaaggca
      901 cgggagaaaa agaagaagta ggagcaagtt caaatttgac agtatatttc gacaagaaag
      961 gagtactaaa agccggaaca atcaccctca aagcaggcga caacctgaaa atcaaacaaa
     1021 acaccaatga aaacaccaat gaaaacacca atgccagtag cttcacctac tcgctgaaaa
     1081 aagacctcac agatctgacc agtgttggaa ctgaaaaatt atcgtttagc gcaaacggca
     1141 ataaagtcaa catcacaagc gacaccaaag gcttgaattt tgcgaaagaa acggctggga
     1201 cgaacggcga caccacggtt catctgaacg gtattggttc gactttgacc gatacgctgc
     1261 tgaataccgg agcgaccaca aacgtaacca acgacaacgt taccgatgac gagaaaaaac
     1321 gtgcggcaag cgttaaagac gtattaaacg caggctggaa cattaaaggc gttaaacccg
     1381 gtacaacagc ttccgataac gttgatttcg tccgcactta cgacacagtc gagttcttga
     1441 gcgcagatac gaaaacaacg actgttaatg tggaaagcaa agacaacggc aagaaaaccg
     1501 aagttaaaat cggtgcgaag acttctgtta ttaaagaaaa agacggtaag ttggttactg
     1561 gtaaagacaa aggcgagaat ggttcttcta cagacgaagg cgaaggctta gtgactgcaa
     1621 aagaagtgat tgatgcagta aacaaggctg gttggagaat gaaaacaaca accgctaatg
     1681 gtcaaacagg tcaagctgac aagtttgaaa ccgttacatc aggcacaaaa gtaacctttg
     1741 ctagtggtaa tggtacaact gcgactgtaa gtaaagatga tcaaggcaac atcactgtta
     1801 agtatgatgt aaatgtcggc gatgccctaa acgtcaatca gctgcaaaac agcggttgga
     1861 atttggattc caaagcggtt gcaggttctt cgggcaaagt catcagcggc aatgtttcgc
     1921 cgagcaaggg aaagatggat gaaaccatca acattaatgc cggcaacaac attgagatta
     1981 cccgcaacgg taaaaatatc gacatcgcca cttcgatgac cccgcagttt tccagcgttt
     2041 cgctcggtgc gggggcggat gcgcccactt taagcgtgga tgacgagggc gcgttgaatg
     2101 tcggcagcaa ggatgccaac aaacccgtcc gcattaccaa tgtcgccccg ggcgttaaag
     2161 agggggatgt tacaaacgtc gcacaactta aaggtgtggc gcaaaacttg aacaaccgca
     2221 tcgacaatgt ggacggcaac gcgcgtgcgg gcatcgccca agcgattgca accgcaggtt
     2281 tggctcaggc gtatttgccc ggcaagagta tgatggcgat cggcggcggt acttatcgcg
     2341 gcgaagccgg ttacgccatc ggctactcga gcatttctga cactgggaat tgggttatca
     2401 agggcacggc ttccggcaat tcgcgcggtc atttcggtac ttccgcatct gtcggttatc
     2461 agtggtaagg gctttttcgc ctgtctgctg ttggggcagg cggaaggttt gaagggaagg
     2521 gtggcgattt gccgcc
//
LOCUS       DAXHTR010000190         2532 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.190, whole genome shotgun sequence.
ACCESSION   DAXHTR010000190 DAXHTR010000000
VERSION     DAXHTR010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.190"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            662..1174
                     /locus_tag="ACFNML_001938"
     CDS             662..1174
                     /locus_tag="ACFNML_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HFC6393250.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAVAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            1254..2333
                     /gene="aroB"
                     /locus_tag="ACFNML_001939"
     CDS             1254..2333
                     /gene="aroB"
                     /locus_tag="ACFNML_001939"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFC6393251.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
BASE COUNT          617 a          763 c          624 g          528 t
ORIGIN      
        1 aacccaaaac ggcaacttaa aaccccgtca ttcccgcgca ggcgggaatc taggtctgtc
       61 ggttcaggac cttatcggat aaaacggttt ctccaaccct gcgttctaga ttcccacttt
      121 cgtgggaatg acgggatatg ggtttccgtg cggacgtgtt cggatttccg cctgcgcggg
      181 aatgacggcg acagatgccc aacggtcttt atagtggatt aacaaaaatc aggacaaggc
      241 gacgaagccg cagacagtac aaatagtacg gaaccgattc acttggtgtt tcagcacctt
      301 agagaatcgt tctctttgag ctaaggcgag gcaacgctgt actggttttt gttaatccac
      361 tatagtattg ataaacatat tatcttcaat atattcaatt ggataattgt ttacctaagc
      421 aaagataatt gccttttcct gacaaataag tgaaatcaac ggattgtcaa aacacagcct
      481 gaaataaaaa acctccctga tttcttttat ttttccttaa aatcagaaag gttcgggatg
      541 gtcgggttat ttttccaaac gtaccgctgc cctgccgatt tcgtataaaa ttccgccgta
      601 acccgacaag cccgaaccct gtcgccccga aaggcggggt gtcaaacatt aaggaattgt
      661 gatgaaaaac tttaacggca aactcatcct catcggactg atgggcgcgg gcaaaaccac
      721 gctgggccgg caaatggcgc agcggctgga ttaccgtttt tacgacagcg atcacgaaat
      781 cgccgtagcc gccggcgttc ccatccccac catatttgaa atggaaggcg aacagggatt
      841 ccgttcgcgc gaaaccgcca tactcaaaaa gctggttatc ctgccccata tcgtcctgtc
      901 caccggcggc ggcgcggtgt taaaagaaga aaaccgcgcc cttatccgca aaagcggcac
      961 ggtcgtctat ctgcacgccc cgcccgaaac cctgctcgaa cgcacgcgct gcgacaacag
     1021 ccgtcctttg ctgcaagttg ccgatccttt ggcgaaatta cgtgaactct acgccgcacg
     1081 cgaccccgtt taccgccaaa ccgccgactt taccgtagaa tcggcaaact gccgggaaac
     1141 cgtgcaaacc ctgctcaaac gcttatcccg ataaaccggc atatgcgccg cgcccagaaa
     1201 accaagccgc gccccgcccg gcgggccggc ggttcaaact ttaaggaaca acaatgaaaa
     1261 cactgaccgt acacaccccg tcccacagct accccatctt tatcggcaac gggctgctgc
     1321 cgcaggcagg aagcctgctc aaaccgcatt tgggcaaacg cgccgccatc atcgccaacg
     1381 aaaccgtcgc cccgctctac ctcggcacgc ttcagacggc attggatgcg gcaggcgtat
     1441 cccatttcag catcatcctg cccgacggcg aggcgcacaa aaactggcag acgctcaacc
     1501 tcatctttga cgggctgatg caaaaccgcg ccgaacgcaa aaccacatta atcgcactgg
     1561 gcggcggcgt gatcggcgac atggtcggct ttgccgctgc cacctaccag cgcggcgcac
     1621 cgttcgttca aataccgacc acactgttga gtcaagtcga ctcatcggtg ggcggcaaaa
     1681 ccgccatcaa ccacccgctc ggcaaaaata tgatcggcgc attctaccag ccgcaggcgg
     1741 tgcttgccga cttggacacg ctgcacaccc tgcccgtccg cgaattgtcc gccggtatgg
     1801 cggaagtcat caaatacggc gcgctcggcg acatcggttt tttcgaatgg ctcgaacaaa
     1861 acatatccgg actgatgacg ctcgatcggg aaaaactcgc ccaagccgtg taccgctgct
     1921 gccaaatgaa ggcagacatc gtcgcccaag acgaaaccga acagggcata cgcgcatggc
     1981 tcaacctcgg acacaccttc ggacacgcca ttgaaaccga gatgggttac ggcacttggc
     2041 tgcatggaga agccatcgcc gccggctgcg tgttggcggc gcgtttgtcc gaacaactgg
     2101 gcaaaacctc cgccgcagat accgcgcggc tcgccgccct gctcgaagcc gccggactgc
     2161 cgtccgcacc gcccgtgttt gcctttgaaa aatggctcga acatatgagc cacgataaaa
     2221 aagtcagcgg cggcatcatg cgctttatcg gtctgaaccg gctgggcgaa gccaacatca
     2281 ccgaaattac cgacacggac atcctccgcc gcaccctgca accgtatctc tgatttcctc
     2341 tgccgatgtg ctgccgcgcg ggtttgacgc acgatgatga ttttccatca tctttctccg
     2401 taaaagcggg aatccggtcc gttcggtttc ggtcgtttcc gataaattcc tgctgctttt
     2461 catttctaga ttcccacttt cgtgggaatg acggcggaga ggtttttgtt gtttcggaga
     2521 agtttctgca ac
//
LOCUS       DAXHTR010000191         2532 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.191, whole genome shotgun sequence.
ACCESSION   DAXHTR010000191 DAXHTR010000000
VERSION     DAXHTR010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.191"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(112..396)
                     /locus_tag="ACFNML_001940"
     CDS             complement(112..396)
                     /locus_tag="ACFNML_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HFC6393252.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
     gene            complement(771..>2532)
                     /gene="pilC"
                     /locus_tag="ACFNML_001941"
     CDS             complement(771..>2532)
                     /gene="pilC"
                     /locus_tag="ACFNML_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
                     /protein_id="HFC6393253.1"
                     /translation="ASEWKKVLLPWTVRGFADDSKFKAFNKEKNNDNKPKYSQRYRIR
                     ENGNNGKRDLGDIVNSPIVAVGEYLATSANDGMVHIFKKGNGGDARDYSLKLSYIPGT
                     MPRKDIQSQDSTLAKELRAFAEKGYVGDRYGVDGGFVLRQVNNLNGQDRVFMFGAMGF
                     GGRGAYALDLTKADGNDPTKASLFDVKDNGNNGNNGNNRVELGYTVGTPQIGKTHNGK
                     YAAFLASGYATKEITSGENKTALYVYDLENNNGTPIAKIEVPNGKGGLSSPTLVDKDL
                     DGTIDIAYAGDRGGKMYRFDLSSQSPDQWTVRPIFEGTKPITSAPAISQLKDKRVVIF
                     GTGSDLSEDDVDKMDEQYIYGIFDDDTETTGNVKVDLKGLGGGLLEQHLTQEDKTLFL
                     TDYKRSDGSGNKGWVVKLKDGQRVTVKPTVVLRTAFVTIHKYTGNDKCGAETAILGIN
                     TADGGKLTKKSARPIVPDANTKVAQYSGDKKTSSGKSIPIGCMQKGNEIVCPNGYVYD
                     KPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLMNDLDS
                     LDITGPTCGMKRISWREVFF"
BASE COUNT          573 a          674 c          578 g          707 t
ORIGIN      
        1 gattcccgcc tgcgcgggaa tgacggttta gaagttgccc gaaacctcaa aaaaaaaaaa
       61 aaaaaaccga aaccgaacaa gccggattcc cgcctgcgcg ggaatgacgg gctaaataat
      121 atcaaaccat aaatcctgcc aagaaacatt attttcttca atcagttgca atttccaagc
      181 cctgttccat ttcttcaact gtttttcccg agtaattgca ctctccatcg taggatgcag
      241 ttcataccaa accagcatag taacgttgta ccgtgatgta aatccctcaa tcaaatgctc
      301 cctatgttgg taaatacgtt gcaccaaatc agatgtaacg ccaatgtata acgtgccatt
      361 acgttggctt gctaaaatat aaaccgcagg ctgcatataa tacccttttg aattatttca
      421 atttatattc ccgcgaacac catcccgtga ttactttaac ccttcgttat tcccatagct
      481 ttccatcatt cccgcaactc ttcgtcattc ccacgaaagt gggaatccag aatctctaaa
      541 gcttcagcta acctttgaat attactgtta tcccaaggtc tagattcccg cctgcgcggg
      601 aatgacggca tcggtttgac ggtatttaat tgaattgtgg aaattgatgg attcagtgag
      661 attggcgaga tgaagcctac cctatagccc gccttttacg aacccgcccc tcccgaaaaa
      721 cgcaaaaaat gccgtccgaa aacctttcgg acggcatttt cgcgtgcaaa tcagaagaag
      781 acttcacgcc agctgattcg tttcataccg cacgtcgggc cggtaatgtc caagctgtcc
      841 aaatcgttca tcagcagggt gcgcacccct ttttgggaga agcagcggtt gtttttgccg
      901 gaacgcttgc cggcggggtc tataccgctg ccgcccgcat cgccgtctgc cgttgttgaa
      961 aatccgtctg ttttcttttc atccagataa cgcacattaa ccggtttgtc gtaaacatat
     1021 ccgttcgggc agacgatttc attgcctttt tgcatacaac ctatagggat ggatttgccg
     1081 ctggaagttt tcttatcgcc ggaatattgc gcgaccttcg tattggcgtc cggcacaatc
     1141 gggcgcgcgc ttttcttggt cagcttgccg ccgtcggcgg tattgatgcc caaaatggcg
     1201 gtttccgcgc cgcatttgtc attacccgta tatttatgga tggttacaaa ggcggtacgc
     1261 aataccacgg tcggtttgac ggtaacgcgc tgtccgtcct tcaatttcac tacccagccc
     1321 ttattgcccg agccgtcgga tcgcttataa tcggtcagga ataaggtttt atcttcctga
     1381 gtaaggtgtt gctcgagcag cccgcctccc aaacctttaa ggtctacctt tacattaccc
     1441 gtcgtctccg tatcgtcgtc gaagataccg taaatatatt gttcgtccat tttatcaaca
     1501 tcatcctcac tcaaatcgct gcccgtgccg aagatgacca cgcgtttgtc tttcagttgg
     1561 gaaatggcgg gcgcggaagt aatcggtttt gtgccttcaa aaatagggcg tacagtccat
     1621 tgatcaggac tttgactgct caaatcaaag cggtacattt tcccgccgcg atcgccggca
     1681 taggcgatat cgattgtgcc gtccaaatct ttatccacca gcgtggggga cgaaagcccg
     1741 cccttgccgt tgggtacttc gatttttgca atcggcgtac cgttgttgtt ttccaaatca
     1801 tacacataca gcgcggtttt attctcgccg ctggtaattt ctttagtcgc ataaccggag
     1861 gcgaggaaag cggcgtattt gccgttgtgg gttttgccga tttgcggcgt gccgacggtg
     1921 tagcctaatt ccacgcgatt attgccgtta ttgccattat tgccgttatc ttttacatca
     1981 aacaaagagg cttttgtcgg gtcattgccg tcggctttgg tcaaatccaa ggcatacgca
     2041 cctctgccgc caaagcccat cgcgccgaac ataaacacgc ggtcttgccc atttaagttg
     2101 ttaacttggc gcaagacaaa gccgccgtcc acgccgtagc ggtcgcccac atagcctttt
     2161 tcggcaaagg cgcgcagctc tttggcgagg gtggagtctt gactttgaat atccttgcgc
     2221 ggcatcgtgc ccgggatgta gctgagcttc agactatagt cgcgcgcgtc cccgccgttg
     2281 ccttttttga agatatgcac catcccgtcg ttggcggaag tagccaaata ctcgccgacc
     2341 gccacgatgg ggctgttgac gatgtcgccc aaatcgcgct tgccgttgtt gccgttttcg
     2401 cggatgcggt atctttggct gtattttggc ttgttgtcgt tgtttttttc tttgttgaat
     2461 gctttaaatt tactgtcatc agcaaaaccc cgaaccgtcc aaggcagcag cacttttttc
     2521 cactcgctgg ca
//
LOCUS       DAXHTR010000192         2526 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.192, whole genome shotgun sequence.
ACCESSION   DAXHTR010000192 DAXHTR010000000
VERSION     DAXHTR010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2526
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.192"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            44..2257
                     /locus_tag="ACFNML_001942"
     CDS             44..2257
                     /locus_tag="ACFNML_001942"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HFC6393254.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPDNDKKLVLAARSLA
                     EAHYPADAVTPYGEPLSDHFLGAAQMVDELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRTELKKYNIHFEVAGRPKHIYSIYKKMVKKK
                     LTFDGLFDIRAVRILVDTIPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSNKAIGKIRAYIRQQNAD
                     TVREEGRVQLDKQLAKLTPKPNLQELAENLGYKKPEDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSELLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
BASE COUNT          676 a          787 c          607 g          456 t
ORIGIN      
        1 gctgttacaa taaactcgtt tttatcggaa cgaaagaccc atcatgaccg ccatcagccc
       61 gattcaagac acgcaaagcg cgactctgca agaattgcgc gaatggttcg acagctactg
      121 cgccgcgctg ccggacaacg ataaaaaact tgtcttagcc gcccgttcgc tggcggaagc
      181 acattacccc gccgatgccg tcacgccgta tggcgagccg ttgtccgacc acttcctcgg
      241 cgcggcacaa atggtcgacg aactcgacct gctgcccgat gccgtcgccg ccaccctgct
      301 tgccgacatc ggacgctacg tccccgattg gaatctattg gtttccgaac gctgcaacag
      361 caccgtcgcc gagctggtca aaggtgtgga cgaagtgcag aaactcaccc acttcgcccg
      421 ggtggacagc ctcgccacgc cggaagaacg cgcccagcaa gcggaaacca tgcggaaaat
      481 gctgctggcg atggttaccg acatccgcgt cgtgttaatc aaactggcga tgcgtacgcg
      541 caccctgcaa tttttaagca acgcccccga cagccccgaa aaacgcgccg ttgccaaaga
      601 aaccctcgac atcttcgccc cgctcgccaa ccgtttgggc gtgtggcagc tcaaatggca
      661 gctcgaagat ttgggcttcc gccatcaaga acccgaaaaa taccgcgaaa ttgccctgct
      721 tttggacgaa aaacgcaccg aacgcctcga atacatcgaa aacttcctca atatcctgcg
      781 tacggaactg aaaaaataca atattcattt tgaagttgcc ggaagaccga agcacatcta
      841 ctccatttac aaaaaaatgg tgaagaaaaa gctcactttc gacggcttgt tcgacatccg
      901 cgccgtacgg attttggtgg acaccattcc cgagtgttat accacgctgg gcattgtcca
      961 cagcctctgg cagccgattc cgggcgagtt cgacgactac atcgccaacc cgaaaggcaa
     1021 cggctataaa agtttgcaca ccgttatcgt cggcccggaa gacaaaggcg tggaagtgca
     1081 aatccgcacc ttcgatatgc accaattcaa cgaatttggt gttgccgccc actggcgtta
     1141 taaagagggc ggcaaaggcg attccgccta cgaacagaaa atcgcttggt tgcgccaact
     1201 cttggactgg cgcgaaaaca tggcggaaag cggcaaggaa gacctcgccg ccgccttcaa
     1261 aaccgagctt ttcaacgaca cgatttatgt cctgaccccg cacggcaaag tcctctccct
     1321 gcccacgggc gcgaccccca tcgacttcgc ctacgccctg cacagcagca tcggcgaccg
     1381 ttgccgcggt gcgaaagtcg aagggcagat tgtgccgctg tccaccccgc tcgaaaacgg
     1441 acagcgcgtc gaaatcatta ccgccaaaga agggcatcct tccgtcaact ggctttacga
     1501 aggctgggtc aaatccaaca aggcaatcgg caaaatccgc gcctacatcc gccagcaaaa
     1561 cgccgacacc gtgcgcgaag aaggccgcgt ccaactcgac aaacagcttg ccaaactcac
     1621 gcccaaaccc aacctgcaag agcttgccga aaatctcggc tacaaaaagc cagaagacct
     1681 ctacaccgcc gtcggacaag gcgaaatttc caaccgcgcc atccaaaaag cctgcggcac
     1741 gctgaacgaa ccgccgcccg tacccgtcag cgaaaccacc atcgtcaaac agtccaaaat
     1801 caaaaaaggc ggcaaaaacg gcgtgctcat cgacggcgaa gacggtctga tgaccacgct
     1861 tgccaaatgc tgcaaacccg cgccgcccga cgacattgtc ggcttcgtta cccgcgatcg
     1921 cggcatttcg gtacaccgca aaacctgccc ctctttccga cacctcgccg aacacgcgcc
     1981 cgaaaaagta ctggacgcaa gttgggcggc gttgcaggaa ggacaagtgt tcgccgtcga
     2041 tatcgaaatc cgcgcccaag accgctccga gcttttgcgc gacgtatccg acgcgctcgc
     2101 ccgccacaaa ctcaacgtta ccgccgtgca aacccagtcc cgcgacttgg aagccagtat
     2161 gcggttcacg ctcgaagtca aacaggtcaa cgacctcccg cgcgtcctcg ccagcctcgg
     2221 cgacgtcaaa ggcgtattga gcgttacccg gctttaaata caaaaatgcc gtctgaaagc
     2281 cgtataacgc ttcagacggc attttgattg acggggtttg ctattttttg ttgcatagtc
     2341 aattaaaaac aaaatagtac aatactcaac tttgaaggtc taaccatggc atactctgcg
     2401 gacttaagaa acaaagcttt aaactatagt ggattaacaa aaatcaggac aaggcgacga
     2461 agccgcagac agtacaaata gtacggcaag gcgaggcaac accgtactgg tttaaattta
     2521 atccac
//
LOCUS       DAXHTR010000193         2507 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.193, whole genome shotgun sequence.
ACCESSION   DAXHTR010000193 DAXHTR010000000
VERSION     DAXHTR010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2507
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.193"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..326
                     /locus_tag="ACFNML_001943"
     CDS             <1..326
                     /locus_tag="ACFNML_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFC6393255.1"
                     /translation="ATTVPAQQTFLDAAELMQWSIETEWLGLNVIAHCNFGKQHAQVE
                     FEACFQDGQHRSAHHELSGFVNIGGQWYFIDPTVPHPAMKQPCICGSGKKFKACCGKY
                     LKPVA"
     gene            complement(545..1339)
                     /locus_tag="ACFNML_001944"
     CDS             complement(545..1339)
                     /locus_tag="ACFNML_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HFC6393256.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSHRKEDGSMLSEADIAAQT
                     AFAAALPLLIDCPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVEWIRENQ"
     gene            1548..2273
                     /locus_tag="ACFNML_001945"
     CDS             1548..2273
                     /locus_tag="ACFNML_001945"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFC6393257.1"
                     /translation="MPRFHLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFDGKG
                     KAHAARLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGDRMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
BASE COUNT          680 a          780 c          559 g          488 t
ORIGIN      
        1 tcgctactac cgttccggcg caacaaacat tcctcgatgc cgccgaactg atgcaatgga
       61 gtatagaaac cgaatggctg ggcttgaatg tcatcgcaca ctgcaacttc ggcaaacagc
      121 acgcccaagt cgaatttgaa gcctgcttcc aagacggaca acaccgatcc gcacatcacg
      181 aactgtccgg cttcgtcaac atcggcggac aatggtattt tatcgatccc accgttccgc
      241 atcctgcgat gaaacaaccc tgcatttgcg gatcgggcaa aaaattcaaa gcctgctgcg
      301 gcaaatatct gaaacctgtc gcataaaaat gccgtctgaa cgttcagacg gcattttcaa
      361 cgtgcaaaaa aaaaccattc ataccaaggg taagtatgaa tggtcaatac attgcgggaa
      421 aacgtcttac ttgctgcact gccgaaaagg gagaaacggc agcggtaatc agcggaaagg
      481 attgtacccg aattaatgtt aagaaacgtt aatcgtgaaa atatattaac aaacctgttg
      541 aaacctattg gttttcccgt atccactcga cccagcgttc aaacagcttc ggttcgagcg
      601 cggcgacgac cgagcgtttg aacacgtgtt caccactcca aaacccgtcg ccttccaaag
      661 tcgtcagcct gccgcccgcc tcctcgaaaa tcaacgcgcc ggcggcataa tcccacagct
      721 tctgcccgcc gtggacataa acatcataac gcccgcacgc cagataacac cagtccaacg
      781 tactgctgcc catactccgt atcgttccaa aaggcgcgag cgtactcatg cggctggaca
      841 gtttgcccga acgcagatat ttgatttcca cgcccgcgat tgcctcattc aacttcttat
      901 ccacgagacg caaaggcagg cgtgtcccgt ttaaaaacgc accctgcccg cgttcagcat
      961 aaaaacattc gccgctgacc ggattgtaga ttacgcccaa ttcggcgcgc ccgttgcgga
     1021 caaacgccac cgacacggca aaatgcggca gcccgttgac aaaattgttt gtcccgtcta
     1081 tcggatcgac aatccacagc cccttttccc ccgaatattg ttcccacaga gccgactgtt
     1141 cctgtcgcga catttcctcg cccaacatcg ggcaatcgat cagaagcggc aacgcggcgg
     1201 caaaagccgt ctgcgcggca atgtccgcct cgctcaacat cgaaccgtct tccttgcggt
     1261 gggacggcgt attcaaaaaa cgcggcataa tttcggtttg cgcgatatgg cgcacgactt
     1321 tctgcaaacg gtgtaacact tcctactgtc ctcatatttt gaacttgcgg cacgcgaacg
     1381 tatatagtgg attaaattta aatcaggaca aggcgacgaa gccgcagaca gtacaaatag
     1441 tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa tccactataa tgtccgtttc
     1501 catcacgccg ctgcgacaga ttataaccgt ccgaaccgcc aaaaactatg ccccgattcc
     1561 acctgcccga aaacctttcc gtcggacaaa ccgtcgccct gcccgacaac atcgtccgcc
     1621 acctcaacgt cctgcgcgtc cgccccaacg aaaacatcac cctcttcgac ggcaaaggca
     1681 aggcacacgc cgcacggctg accgttttgg aaaaacgccg cgccgaagcc gaaatcctgc
     1741 acgaagacac aaccgacaac gagtccccgc tcaacatcac actgatacaa tccatctcct
     1801 ccggcgatcg catggatttc accctgcaaa aaagcgtcga actcggcgta accgccatac
     1861 agcccgtcat cagcgaacgc tgcatcgtcc gcctcgatgg ggaacgcgcc gccaaacgcc
     1921 tcgcacgctg gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg aacaccgttc
     1981 cccccgtgct gcccatcatc ggctatcgtg aagcactcga caaaatgccg tctgaaagca
     2041 ccaagctgat tatgagcatc aaccgcgccc gcaaactcgg cgacatccgc cacccgtccg
     2101 gcgcaatcgt ctttatggtc gggcccgaag gcggctggac agaacaggaa gaacaacagg
     2161 catttgaagc tggctttcag gcggttacac tcggcaaacg gattttacgc acagaaaccg
     2221 ccccactcgc cgccctcgcc gccatgcaga cgctttgggg cgatttcgca taaacagaaa
     2281 tgccgtctga aacccgttca gacggcattt tgcagccgat taagatagta ggttcaaata
     2341 agatttcccg tgtcgtcatt cccgcgaaag cgggaatcta gaaacgaaaa actacaggga
     2401 tttatccgaa acaacaaacc ctctccgcca tcattcccgc aaaagcggga atctagaaac
     2461 gaaaaactac agggatttat ccgaaacaac aaaccctctc cgccgtc
//
LOCUS       DAXHTR010000194         2301 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.194, whole genome shotgun sequence.
ACCESSION   DAXHTR010000194 DAXHTR010000000
VERSION     DAXHTR010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.194"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            136..2016
                     /locus_tag="ACFNML_001946"
     CDS             136..2016
                     /locus_tag="ACFNML_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNB domain-containing ribonuclease"
                     /protein_id="HFC6393258.1"
                     /translation="MNIFYEESGQFKVAAVVQKNDATYQVDTQHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFSAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLALPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLGNGMKRVGIHIAAPS
                     LAIKPGDKMEQIIMQRLSTVYFPNSKITMLPENWIAAFSLDAGAYRPAVSIYFDVDGE
                     FNVGAPTCKIEAVNISENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFAITL
                     QKARGKYEPNRAPQYDYSIELDEEGKVSVVRRERGSPIDTLVSEMMILANSTWAQMLH
                     DNDLPGLFRVQPTGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     TAEPLYQNSDAELFAALRDFDAAYTAYADFQHQMEAYWSLVYLQQQGISELTATILKE
                     DLVRIEGLPLATRANGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGKTAGN
                     AV"
     gene            complement(2062..>2301)
                     /locus_tag="ACFNML_001947"
     CDS             complement(2062..>2301)
                     /locus_tag="ACFNML_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393259.1"
                     /translation="VGMTIQKLPETKKLKPNGLDSRFRGNDGISVSVRINLDSHLRGN
                     DGILDCGYLSGTAAWKFIETEKQRKPKKPDSRLWE"
BASE COUNT          609 a          690 c          545 g          457 t
ORIGIN      
        1 gtactggttt ttgttaatcc actataacgc aagcaccgca aagacgcgcc aaccctctcc
       61 caaccttttt tcagacggca ttttcggtaa tctgctaaaa tcgcccgctt gagtttccac
      121 agaaaaatcc gaaaaatgaa tattttttac gaagagtccg gccagttcaa agttgccgcc
      181 gtcgtccaaa aaaacgatgc cacctaccaa gtcgataccc aacacggcaa gcgcaccaaa
      241 gtgaaggcaa acaatgtctt tgccgagttt gacggcgata tggcggcgtt tttggaaaac
      301 gcgcaggcgc aggcggcgga catcgacacc gatttattgt gggaggtatg cggcgaagaa
      361 gaattttccg ccgaagctat cgccgaagaa tattacggac acgcgccgac caaaaccgag
      421 ctggcggcga ctttgattgc actttacgcc gcgccgatgt atttctacaa aaaagccaaa
      481 ggcgtgttca aagccgcgcc cgaagaaacc ttaaaacaag cgcttgccgc catcgaacgc
      541 aaaaaacaac aagacgcgca aatcgacgct tgggcggaag ccttgaaacg cggcgagatg
      601 ccgtctgaaa tcgcggcgga tttgaaaacc atcctgcacg cgcccgacaa gcaatcattg
      661 acctacaaag cctttaccaa agccgccgac gcattgaaaa cctccgccta cgaattggcg
      721 aaaaaaacgg gcggcattac gtccattccc caatacctgc aagacggttt tgaaatcaaa
      781 tacttcccca aaggcacagg cttccccgac cttgcccttc ccgaaatgcc cgacttgcct
      841 aaggccgacg ttaccgcctt ttccattgac gacgaatcga ccaccgaagt ggacgacgct
      901 ttaagcctga ccgacttggg caatggcatg aaacgtgtcg gcatccacat cgccgcgccg
      961 tcgcttgcca ttaaaccggg cgacaaaatg gaacaaatca ttatgcagcg gttgagtacg
     1021 gtttattttc ccaacagcaa aatcactatg ctgcccgaaa actggatagc cgcgttcagc
     1081 ctcgatgcgg gcgcgtaccg ccctgccgtc agcatttatt tcgatgtgga cggcgagttc
     1141 aacgtcggcg cgccgacctg caaaatcgaa gcggtcaaca tctccgaaaa cctgcgtatc
     1201 caagccatcg aaccgcattt caacgccgaa gcgggcttgg acgaagcggg cgaaatgatg
     1261 ttcgcccacc atcaagacct gatttggttt tatcaattcg ccatcaccct gcaaaaagcg
     1321 cgcggcaaat acgagcccaa ccgcgcgccg caatacgatt acagcatcga attggatgag
     1381 gaaggcaagg tatccgtcgt ccgccgcgaa cgcggttcgc ccatcgatac gctggtcagc
     1441 gagatgatga ttcttgccaa cagcacttgg gcacaaatgc tccatgacaa cgacctgccc
     1501 ggcctcttcc gcgtccaacc caccggaaaa gtgcgcatga gcaccaaatc cgagccgcat
     1561 atcggcatgg gcgtgcagca ttacggctgg ttcacttcgc cgctgcgccg cgccgccgac
     1621 tacatcaacc aaaagcagct gatcagcctg atagacgaca ctgccgaacc gctgtatcaa
     1681 aacagcgatg ccgagctttt cgccgccctg cgcgactttg atgccgccta tacagcctac
     1741 gccgacttcc aacaccaaat ggaagcctac tggagcctgg tttacctgca acagcaaggc
     1801 atcagcgagc tgacggcgac gattctgaaa gaagacctcg tccgcatcga aggtctgccg
     1861 ctggcaacgc gcgccaacgg catcccgttt gacgcgctgc ccaaatcgca ggcattgttc
     1921 aaaatcaccg aattagatgc cgagaagcag tttgtctcgc tcaactacat caaggcagtc
     1981 gcacccgcag gcaaaacggc aggcaatgcc gtctgaagcc cgatacggca aactccggca
     2041 aaaacagaaa cctgaatctc atcattccca cagtcgggaa tctggttttt tgggtttccg
     2101 ttgtttttcc gtttcaatga acttccaagc cgccgttccc gataaatacc cgcaatctaa
     2161 aatcccgtca ttcccacgca gatgggaatc taggtttatc cgcacagaaa ctgatatgcc
     2221 gtcattcccg cgaaagcggg aatccagacc gttcggcttc agttttttgg tttcgggcaa
     2281 cttctgaatc gtcattccca c
//
LOCUS       DAXHTR010000195         2287 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.195, whole genome shotgun sequence.
ACCESSION   DAXHTR010000195 DAXHTR010000000
VERSION     DAXHTR010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.195"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            20..748
                     /gene="rpe"
                     /locus_tag="ACFNML_001948"
     CDS             20..748
                     /gene="rpe"
                     /locus_tag="ACFNML_001948"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFC6393260.1"
                     /translation="MTTYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPYTLEKIRQV
                     RAMLDRYEEKSGRRIAIEVDGGIKTDNIAAAAQAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="ACFNML_001949"
     CDS             complement(771..1802)
                     /gene="ruvB"
                     /locus_tag="ACFNML_001949"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFC6393261.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(1884..2192)
                     /locus_tag="ACFNML_001950"
     CDS             complement(1884..2192)
                     /locus_tag="ACFNML_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393262.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
BASE COUNT          461 a          535 c          737 g          554 t
ORIGIN      
        1 gatattgaag gaaaaaaaca tgactactta ccgtatcgca cccagcattt tatcggcaga
       61 ttttgcacgg cttggcgaag aggtggaaag cgtcattgcg gcaggtgcag atctgatcca
      121 ttttgacgtg atggacaacc attatgtgcc gaacctgacc ttcgggccta tggtttgcgc
      181 ggcgttgaag ccttatgcaa gcgtgccgat cgatgtgcat ctgatggtcg aacccgtgga
      241 tgacctgatt cagtcgtttg ccaaagcagg agcatcaatc atcacgttcc atcccgaagc
      301 gagccgccat attgaccgca gcttgagcct gattcgggac atgggctgtc aggcggggct
      361 ggtgttgaat ccggcaacgc ccgtatatct gttggaaaac gtattggaca ggctggatat
      421 ggttttgctg atgtcggtaa accccgggtt cggcggtcag agcttcattc cgtatacttt
      481 ggaaaaaatc agacaggttc gtgccatgct tgaccgctat gaagagaaaa gcggcagacg
      541 tattgccatt gaagtggacg gcggcatcaa aaccgacaat atcgccgccg ccgcccaagc
      601 cggcgcggat acctttgttg ccggttctgc gattttcggc aaaccagact ataaggcggt
      661 gattgatgcg atgagggcgg aactggaaaa aagcggcagg ctgaaccttg cgccaatgat
      721 tgaaatagaa atgccgtctg aaaattagct ctgtttcaga cggcattgcg ttatttttcg
      781 acgggcagcc cgaaatgcag gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt
      841 tgcaggaagc cttgttggat aaggtagggt tcgataacgt cttcgatggt gtctgtagat
      901 tcgccgatgg cggcggcaac attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa
      961 acggcttcga gaaatttcct gtccatcacg tccagcccct gcgcgtccac gtccagcata
     1021 cttaaagcgg catcggcgat gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa
     1081 tcgcgcacgc gtcgcaacag gcggttggcg atgcgcggcg taccgcggct gcgtttggcg
     1141 atttcttccg cgccttcttc ggacatatcg agctgcaaca gttgtgccga acggctgacg
     1201 atggtggtaa ggtctcggtt ttcgtaaaac tcaaggcggg agacgatgcc gaagcggtcg
     1261 cgcaacggat tggtcagcat accggcgcgg gtggtcgcgc cgatgagcgt gaagggcggc
     1321 aggtcgattt tgacggaacg ggcggcgggt ccttcgccta tcataatgtc gagccggtag
     1381 tcttcgagcg cgggatagag gatttcttcg acaacagggc tgaggcggtg gatttcgtcg
     1441 atgaacaata catcgtgcgg atcaaggttg gtcaaaaggg cggcgaggtc gcctgcgcgt
     1501 tcgaggacgg ggccgctggt ttggcgcaaa tttacgccca attctttggc gatgatgtgc
     1561 gccagtgtgg ttttgcccag tccgggcggg ccgaagagca aaacgtggtc gagtgcttcg
     1621 ccgcgttttt tggcggcttg gatgaaaatg gcaagctgtt ctttggcttt gtcttgcccg
     1681 atgtagtcgt ccagcgtttt ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag
     1741 gcggtttggg cggcaacgat gcgttgcggt tgcgcggcgg tcaggttgtc ggtttgcagc
     1801 atagtgttcc ctttgtcggg tatgccgtcc gaacggtcgg cggcgtttca gacggcattg
     1861 aaaagataac ggtcaaaagc gttttatccg cgggtttcgc gccaaacgag atagtcgcgc
     1921 aggggcggca taggcgggtt gatgcagtgg gggcagatgc cggtggtgtc ttcgtcgtcg
     1981 ctgtcgatgt ggtaggcgtt ggggtcgaag cggatttgct gcatgacggc ttgcgccaat
     2041 gcctgcgcct gaacaacgtc gaaattgaat ttttcgagga tttcgttcaa caggcggatt
     2101 tcgccaccgc taaattccgc actgtgtttg aggatgaggc gttgataatc ttcgttgttg
     2161 attttaggca ataaatcggt tttcagggtc atgattttat gtcgggggca ggatggctga
     2221 agcgtgaatt atagcggata tggcggcggc tgttgcaaag cttggattgg gtatcgtcct
     2281 caaaact
//
LOCUS       DAXHTR010000196         2172 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.196, whole genome shotgun sequence.
ACCESSION   DAXHTR010000196 DAXHTR010000000
VERSION     DAXHTR010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.196"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            104..1915
                     /gene="typA"
                     /locus_tag="ACFNML_001951"
     CDS             104..1915
                     /gene="typA"
                     /locus_tag="ACFNML_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HFC6393263.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQVVAVMNHDQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(1927..>2172)
                     /locus_tag="ACFNML_001952"
     CDS             complement(1927..>2172)
                     /locus_tag="ACFNML_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393264.1"
                     /translation="LPKLVRTQKAYSFKRHCSTCCREAEPDTETRRGDIPLEKWRQFL
                     DWVGKTPSETASRSRKARPNKKCRLKFFRRHRVSFKH"
BASE COUNT          544 a          622 c          564 g          442 t
ORIGIN      
        1 gaaaggcaag ggcttcagac ggcatcttca tttcccaaat actgtccggt aaagcgtggt
       61 ataatgcgca gttatctctt acagtttttg aaaaacatta attatgaaac aaatccgcaa
      121 catcgccatc atcgcccacg tcgaccacgg caaaaccaca ttggtcgacc aactgctgcg
      181 ccaatccggc acattccgcg ccaaccagca ggttgacgag cgcgtgatgg acagcaacga
      241 cctcgaaaaa gaacgcggca tcaccatcct cgccaaaaac accgccatcg attacgaagg
      301 ctaccacatc aatatcgtcg acacgccggg acacgccgac ttcggcggcg aagtggagcg
      361 cgttttgggg atggtggact gcgtcgtctt gttggtggac gcgcaggaag gcccgatgcc
      421 gcaaacccgt ttcgtgacca aaaaagcctt ggctttgggg ctgaaaccga ttgtcgtcat
      481 caataaaatc gacaaaccgt ccgcccgtcc gagctgggtc atcgaccaaa ctttcgagct
      541 gttcgacaac ttgggcgcga ccgacgagca gttggatttc ccgattgttt atgcttccgg
      601 tctgtccggt ttcgccaaat tggaagaaac cgacgagagc aacgacatgc gtccgctgtt
      661 cgatactatc ttaaaatata cgcctgcacc gagcggcagc gcggacgaaa cgctgcaact
      721 gcaaatttcc caactcgact acgacaacta caccggccgc ctcggtatcg gtcgtatctt
      781 gaacggacgt atcaagcccg gtcaagttgt tgccgtcatg aaccacgatc aacaaatcgc
      841 ccaaggccgc atcaaccagc ttttgggttt caaaggttta gaacgcgtgc cgcttgaaga
      901 agccgaagcc ggcgacatcg tgattatttc cggtattgaa gacatcggca tcggcgtaac
      961 catcaccgac aaagacaacc ccaaaggcct gccgatgttg agcgtggacg aaccgacgct
     1021 gaccatggac tttatggtta acaccagccc gttggcgggt acggaaggca aattcgtaac
     1081 cagccgccaa atccgcgacc gcctgcaaaa agaattgctg accaacgtcg ccctgcgcgt
     1141 ggaagacacc gccgatgccg acgtgttccg cgtatccgga cgcggcgagc tgcacctgac
     1201 cattttgttg gaaaatatgc gccgcgaagg ctacgaactc gccgtcggca aaccgcgcgt
     1261 cgtgtaccgc gacatcgacg gtcaaaaatg cgaaccttat gaaaacctga ctgtggacgt
     1321 acccgacgac aaccaaggcg cggtaatgga agaactcggc cgccgccgtg gcgaactgac
     1381 taatatggaa agcgatggca acggacgcac ccgcctcgaa taccatattc cagcgcgcgg
     1441 cttgatcggt ttccaaggcg aattcatgac cctgacgcgc ggtgtcgggc tgatgagcca
     1501 cgtgttcgac gattacgcgc ccgtcaaacc cgatatgccc ggccgccaca acggcgtgct
     1561 ggtgtcccaa gagcagggcg aggcggtcgc ttacgccttg tggaatcttg aagaccgcgg
     1621 ccgtatgttc gtatcaccca acgacaaaat ctacgaaggt atgattatcg gcatccacag
     1681 ccgcgacaac gatttggtgg tcaacccgct caaaggcaaa aaacttacca acatccgcgc
     1741 cagcggcacc gacgaagccg tgcgcctgac cacgccgatc aagctgacgc tggaaggtgc
     1801 ggttgagttt atcgacgatg acgaactcgt tgaaatcacg ccgcaatcca tccgtctgcg
     1861 caagcgttac ttgagcgaat tggaacgccg ccgccacttt aaaaagctgg attgatgttt
     1921 actggattaa tgtttaaatg atacgcgatg ccgtctgaaa aatttcagac ggcatttttt
     1981 attcggacgg gctttgcggc ttcttgaagc tgtttcagac ggcgtttttc ctacccaatc
     2041 aagaaactgc cgccattttt ccagcggtat atcgccccgt cgcgtttcgg tatcgggttc
     2101 ggcttcccgg cagcatgtcg aacaatgccg tttgaaggaa tacgcctttt gagtccttac
     2161 aagcttagga ag
//
LOCUS       DAXHTR010000197         1944 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.197, whole genome shotgun sequence.
ACCESSION   DAXHTR010000197 DAXHTR010000000
VERSION     DAXHTR010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.197"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            244..1701
                     /locus_tag="ACFNML_001953"
     CDS             244..1701
                     /locus_tag="ACFNML_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HFC6393265.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPIVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKCYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          435 a          579 c          446 g          484 t
ORIGIN      
        1 ccgccctatc ggataagccc ttaacagaaa aggctttacc cgcgccgtat cggaacacat
       61 cctctaaaat acaatccgtt gaattgaaaa aaatataaaa acatccgccc gcgaaaaacg
      121 gcagcgcgtc gtttgacaaa gaatgaaaat atcggttaaa aaccgatttt catacaaaaa
      181 acaccgctgc cgtccgcatc cgtttcagac ggtattgaga gaaaatcttt taggagaacc
      241 tttatgtccc ggcatcccgc ccccaccgga gaaaaaacat tcttcggcca ccccttccag
      301 ctttccaccc tcttccatat cgaattgtgg gaacgttttt cattttacgg aatgcagggc
      361 atcctgctga tttacctcta ctacaccgcc gacaaaggcg gcttgggcat agacaaaacc
      421 ctcgccggcg gcattgtcgg cgcatacagc ggcagcgtgt acctgtccac cattttgggg
      481 gcgtggtttg ccgaccgagt atggggtgcg gaaaaaaccc tcttcctctc gggcatcgtc
      541 gtgatgctcg gacacatcgt ccttgccgcc gccccgggcc tgtacggcct tttaatcggg
      601 ctgatattca tcgcattggg cagcggcggc gtgaaatcta cggccagttc tatggtgggc
      661 gcattatacg aacaggacga aatgcggccg ctgcgcgatg cgggattttc cattttctac
      721 atcgccatca atatcggcgg cttcctaggc ccgctgctga ccggcctgct gcaggaaaac
      781 atcggtttcc attatggttt cggcgcggcg gcggtcggta tggcattcgg cttgtggcgt
      841 tattccttgg gacgtaaaaa cctgccccat cccatcgtcc cccatccgct ttcaaaagga
      901 cagggcaaaa ctgcggccgc cgtcggcatt gctctcatcg ccgcacttgc aaccgccatc
      961 aaaaccgggc ttgtcaacct cgacaatttc tccggcatcc tcttatctac cgtcatcctt
     1021 gccgtcattg cctattttgc ccgcctgctg actaatcccc gcgtcagttc cgacaacaaa
     1081 cggcacatca tcgcctacat cccgcttttc ctgaccatct gtatgttttg ggccgtctgg
     1141 tttcagattt acaccgtagc aaccgtctat ttcgacgaaa ccgtcaaccg caccatcggt
     1201 tcgtttaccg tgcccgtcgc ctggaaagat tccatgcaaa gcctgtgggt catcctgttt
     1261 tccggactga tggcggcaat gtggacaaaa atggggcgca aacagcccaa aaccccgctg
     1321 aaattcgcta tggcggtatt tgttaccggc gcgtcgtttt tgggattcgt cccctttatt
     1381 tccgccggca cgccgatgcc tattgcggtt ttcgcactga ttgtccttgc catcacgata
     1441 ggcgaactga tgatttcccc gattgcgctg tccatctcca ccaaaatcgc accgccttta
     1501 ttcaaaaccc aaatggtcgc ccttaatttc cttgcctttt cattaggctt cactttgggc
     1561 ggcgtattgt ttgaaaaatg ctatcaggcg ggcgacgaaa tcggcttcta tcggctgctg
     1621 ttctacatcg gcgcagccac aggcttcctg ctgctcctgc tcgtccccaa attgaacaaa
     1681 atgctcgaag gcacagacta agtcccgccc cgatgccgtc tgaacccttc agacggcatt
     1741 tttccgcata atgaaaccaa accgtttcca cccgacagga caggctcccc ccaaccggaa
     1801 ggcagcctgc cgattgtcat ttaaatatct gactgattcc gtctgtttgc aagggaaata
     1861 gccgtttatt tccgcttggg cggaaaccgg ttctattgag taaaaggcat tttatccgac
     1921 taaattagtg ctgcatcaac ggaa
//
LOCUS       DAXHTR010000198         1843 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.198, whole genome shotgun sequence.
ACCESSION   DAXHTR010000198 DAXHTR010000000
VERSION     DAXHTR010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.198"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            53..1222
                     /gene="metZ"
                     /locus_tag="ACFNML_001954"
     CDS             53..1222
                     /gene="metZ"
                     /locus_tag="ACFNML_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HFC6393266.1"
                     /translation="MSKKLHPQTLAIRGGKEQTGYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            1317..1523
                     /locus_tag="ACFNML_001955"
     CDS             1317..1523
                     /locus_tag="ACFNML_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HFC6393267.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(1622..1734)
                     /locus_tag="ACFNML_001956"
                     /pseudo
     CDS             complement(1622..1734)
                     /locus_tag="ACFNML_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT          445 a          480 c          477 g          441 t
ORIGIN      
        1 aaacaacggg cgcacgctcc gttgccgaac ggataaaacc gaggaatcac cgatgagcaa
       61 aaaactccat ccgcaaacgc tcgccatacg cggcggcaaa gaacaaaccg gataccgcga
      121 acacaatcag gcattgttcc tgaccagcag tttcatgtgg gacaacgccc aacacgctgc
      181 cgatttgttt tcaaaaaaaa tcaaaggctt cacttatacc cgtaccgcca acccgaccac
      241 agccgccttt gaaaaacgca tcgccgcttt ggaaggtgcg gaacgcgcgg tcgccacttc
      301 gaccggcatg tccgcgattc aggcggcgtt tttcaccttt ttgcaggcgg gcgaccatgt
      361 gatttccagc cgcagcctgt tcggcacgac cgtcggcttt atcaataaca tcgttaccaa
      421 attcggcatc gaagtgagtc acgtgtcgcc gactgatata aacgaatgga aagccgccgt
      481 caaagccaac accaaactgc tgtttttgga aacgccgtcc aatcccttgg gagaagtggc
      541 cgacttggaa gccttggcgg aattggcgca cggcatcggc gcgctcttgg tggtggacaa
      601 cagcctgctg tcgcctgtcg gctctcagcc tttgaaacac ggcgcggata tttccgtttc
      661 ctccgcgacc aaagccatag acggacacgg gcgcgtgatg ggcggcgtgt tggcgggttc
      721 ggaagaactg atggcgcagg tcgccgttta ctgcaactct tgcgggctgg caatgtcgcc
      781 gtttaacgca tggcagttgt tgagcggcgt ggaaaccctg tctctgcgta tggaaaaaca
      841 gttcgacaac gccttgaaaa tcgcgcaatg gctgcaagcg cagccgcagg ttcaagccgt
      901 gtattacacc ggattacccg accatcctca ggcggagctt atccgcaaac agcaaaacgg
      961 cggcggcatc gtcatcggct ttgaagtcgc cgaccaagca gccgcgtgga aagtcgtgga
     1021 cggcgtcgaa cttttctccc gtaccgccaa cctcggcgac gtgcgctcga ccattaccca
     1081 tccgtggaca accacgcacg gcaggatgca gcccgaagaa aaactcgccg ccaacatccg
     1141 ccccggattg gtgcgcctgt cggtcggttt ggaatatgtt ggcgatttga ttgacgattt
     1201 gaaacaggca ctcgcgcgct gattgcgtgt tcagacggca tcggcggaaa aacaccgcaa
     1261 atgccgtctg aaacccttat cttatatgac atacacgtca acaataggaa aaaaatatgg
     1321 atgaagcggt ttttgccgat tgggcgttga aaatctgcct gaccggcttg attatttttt
     1381 tgggttttat cgtttggaat ttgggcaagg agtctaaagc gggcaaattc ggcatcgcca
     1441 ttttgttttt ggttttgggc ttgggcgtgt ttggttttgt gttcaaagag ttattgatta
     1501 agtttttggt attgccgaaa taggtttgcg gtctgtcaaa atgccgtctg aaatcataaa
     1561 aagtaatctg caccctaaaa gtttgattta accgccaact gattaaggtg cggattttta
     1621 tgacaaaata tagtggatta acaaaaacca gtacggcgtt gcctcgcctt gccgtactat
     1681 ttgtactgtc tgcggcttcg tcgccttgtc ttgatttaaa tttaatccac tataaaaatc
     1741 atctttccgc agtcattccc acggatgcga aagcctgtct ttttgaatcc cggataattt
     1801 tacatcgtca ttcccgcgca ggtgggaatc cagatcattg ggt
//
LOCUS       DAXHTR010000199         1801 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.199, whole genome shotgun sequence.
ACCESSION   DAXHTR010000199 DAXHTR010000000
VERSION     DAXHTR010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.199"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            455..1801
                     /gene="pilC"
                     /locus_tag="ACFNML_001957"
                     /pseudo
     CDS             455..1801
                     /gene="pilC"
                     /locus_tag="ACFNML_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259994.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
BASE COUNT          487 a          472 c          462 g          380 t
ORIGIN      
        1 acggtttttt tgagatttta cgttccggat tcccgctttc gcggcaatgg cgggccaagg
       61 aggcggttgc ggattgccga aaaccgcttt accatgatca gcgcgcaagg gtagaataat
      121 gcggtaaccc tatacattgc atccggccaa aggggcgtgt tacttttccg aacatccccc
      181 tttggcagcc gggccaaagg ggttttccaa ccagcaattc ggcgggcgcg gaatcgggcg
      241 gtttgccgaa tcccgccgtt tgcggcgcgc ctgccgccga cggtatcccg cgaagcaaga
      301 tttaagggat aaaatatgtt ccaacacgca gggcggcaca taaggcgccg ccctgattcg
      361 gaagggcttg cacccctccc ggacaaagcc tgatcctgcc gccccgaagg acggatgccc
      421 gaagggcggg gggtttgacc gaaaaggaaa tacgatgaat aaaactttaa aaaggcaggt
      481 tttccgccat accgcgcttt atgccgccat cttgatgttt tcccataccg gcgggggggg
      541 ggggggcgcg cgcaggcgga aacccgtaac tacgctattg tcatgaacaa acaaaatcag
      601 ccggaggtaa agcaggaggg ttcatattca accttaaggg aaaaagacag gaaacgcaaa
      661 tttgacttta atgcgaacaa cgggggtgga ggctctgtct cattcaacaa tagcgatgag
      721 cttgtttctc gacaaaacgg tactgccgtt tttggcacag ccacctacct gccgccctac
      781 ggcaaggttt ccggttttga tgacaaaagg ctgaaagagc gcggcaatgc cgttaattgg
      841 attcatacga cccacccagg gttgataggc tacagctacg ccggtgtcgt atgcagaaac
      901 agcacaggct gtccccaact tgtctataaa acccgatttt ccttcgacaa caccggtttg
      961 gcaaaaaatg cgggcatgct ggatagtcac ccggacccaa gccgcgaaaa ttcgcccatt
     1021 tacaaattga aagattatcc atggttgggc gtatctttca atttgggcag cgagaatacc
     1081 gtcaaaaata gccaatcatc caacagattg atatcttctt ttagtgaaaa caataataat
     1141 caaaccatcg tctctacgac agaaagccac cctatttccc ttggcgacgg gcagcgcgaa
     1201 cataccgccg tggtctatta tctgaacgcc aagctgcacc tgctgaataa aaaacagatt
     1261 caaaatatca ccgacaaaac agtgcagttg ggtgtcttga agccgagcat cgatgtgaag
     1321 acacaaagga cggggtttgg cggcattcta tctttttggg ctagttggga cattaaagat
     1381 accgggcaga ttccagtcaa gctcggcctg cagcaagtca aagcaggccg ctgcgtcaac
     1441 gcaaataacc ccaataagag taccaaagcc ccttcccccg ccctgactgc ccccgcgctg
     1501 tggttcggac ctgtgcaaaa tggtaaggtg cagatgtatt ccgcttcggt ttccacctac
     1561 cccgacagtt cgagcagccg catctacctt caaaatctga aaagaaaaga cgaccccggc
     1621 agacccggcc gctattccct cgcaaccttg aataagtcgg atattgaaag tcgagagccg
     1681 actttcacag ggcggcaaac cgtcatccga ttggataaag gcgtacatca gatcaaactt
     1741 aaaggcaatg aggtcgaagg ttttaaggga aacaacggca acgacacttt cggcattgtt
     1801 a
//
LOCUS       DAXHTR010000200         1791 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.200, whole genome shotgun sequence.
ACCESSION   DAXHTR010000200 DAXHTR010000000
VERSION     DAXHTR010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.200"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(148..804)
                     /locus_tag="ACFNML_001958"
     CDS             complement(148..804)
                     /locus_tag="ACFNML_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HFC6393268.1"
                     /translation="MKNKSKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFILFLLVFRLCWGIWGSDTARFSRFVRGWSGIRDYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQIGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFNLLAVFSAIH
                     IAAVAAYRVFKKKNLVLPMITGFKYIEGKTSIRFAGKTALAAALSVAALAAAAILLLS
                     "
     gene            869..1759
                     /locus_tag="ACFNML_001959"
     CDS             869..1759
                     /locus_tag="ACFNML_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HFC6393269.1"
                     /translation="MFPVFHLSGESRRRMLQAALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLTKRQG
                     INAWVVGCGLGTDKAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIRAIFGATVVLKGHKTLVASPDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
BASE COUNT          406 a          425 c          583 g          377 t
ORIGIN      
        1 ccttgtcctg atttttgtta atccactata aaacggtttg ggacggaaaa acggcctgaa
       61 gccgtttcgg gcattcagac cgtttgcgcg gagaggggat gccgtccgaa gggcgaaaag
      121 ggcttcagac ggcattgatg tcgggtttca ggacaggagc aggatggcgg ctgcggcaag
      181 cgcggcaacc gataatgcgg cggcaagcgc ggttttaccg gcaaagcgga ttgaggtttt
      241 gccttcgatg tatttgaagc cggttatcat cgggaggacg aggtttttct ttttgaacac
      301 gcggtatgcg gcgacggcgg cgatatgaat ggcggaaaaa acggcgagca ggttgaaaaa
      361 gttgaggtgg attttccgca taaggctgcc tgtatgttcg gaaaccaaat ggttgaggta
      421 gccgttggtg ctgaaggtgt tttcatcggc ggcaaaaagc cccgtgccga tttgaaatga
      481 gacggcggcc aaaagcgcga cgaccatcag tgcgcccaag gggttgtgtc cgggctggat
      541 atgttcggga ataccgtttt tcagataatc cctgataccc gaccatccgc ggacgaaacg
      601 ggagaaacgg gcggtatcgc tgccccaaat gccccagcag aggcggaata cgagcaggaa
      661 aaggataaac agcccgacgc gcgtgtgcca ttgaagcata tcgccgccgg cttttgcgct
      721 ataccacata aagggcaagg acgcggcaag cagccagtgg aaaaggcggg tggggaggtc
      781 ccagactttg cttttgtttt tcataatcgg tttccggcgg tagaatcggt ttgttttcga
      841 gccttaattt taaccgattg gaggggaaat gtttcccgtt ttccatcttt caggcgaaag
      901 ccgccgccgg atgcttcagg cggcattgcg ttttccctat gttttcaaag cccgtgcgga
      961 agattcgcac aaagggactt tcggcacgct cgccgtagtc ggcggatcgg cagggatgag
     1021 cggtgcgccc gtattggcgg catcggcggc gatgtatctc ggctgcggca aggtgtgggc
     1081 gggtttcaat caggatgcgc ttccgtttgc cgtcattgcc ggtttccccg aaattatgtt
     1141 ggacacggca gacggtttga ccaagcgtca aggcataaac gcctgggttg tcggttgcgg
     1201 cttgggtacg gataaggcgg cggtcggaac gcttgccgga attttgacgg aacacacgga
     1261 caagcccgtc cttttggatg cggacgcgct gaacatatta tcaaccgatg ccgaaacccg
     1321 aaatctggca cgcgggtgta aaaacctgat tttaacgcca caccccgccg aagccgcgcg
     1381 cctgcttgga acgacggttg cacaggttca ggcggatcgg acggcggcag tgaggaagat
     1441 aagggcaatt ttcggcgcaa ccgtggtttt aaaggggcac aaaacattgg ttgcctcacc
     1501 cgatacggaa atctatgtca acgaaagcgg caacgcggga ttggcaacgg cgggcagtgg
     1561 cgacgtattg ggcggcatca tcggcagtct gctcgcacag ggcgtgccgg tttttgaagc
     1621 cgcctgcgcg ggcgcgtggc tgcacggcgc ggcggcggat gtcataaaag aatcggcagg
     1681 cattgcggca gggctgttgg ctggcgaaat cgccccggcg gcaaggtggc tgcgcaaccg
     1741 gataactgaa agtatgtaag aagatatagt ggattaacaa aaaccagtac a
//
LOCUS       DAXHTR010000201         1771 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.201, whole genome shotgun sequence.
ACCESSION   DAXHTR010000201 DAXHTR010000000
VERSION     DAXHTR010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.201"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(122..550)
                     /locus_tag="ACFNML_001960"
     CDS             complement(122..550)
                     /locus_tag="ACFNML_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mor transcription activator family protein"
                     /protein_id="HFC6393270.1"
                     /translation="MGFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(531..965)
                     /locus_tag="ACFNML_001961"
     CDS             complement(531..965)
                     /locus_tag="ACFNML_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HFC6393271.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
     gene            complement(1122..>1771)
                     /locus_tag="ACFNML_001962"
     CDS             complement(1122..>1771)
                     /locus_tag="ACFNML_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFC6393272.1"
                     /translation="QMTAQKEDSQTMPYQPPTEAEEVLEALVNRRNQPADMRTAEKNR
                     LHQVHETQVGSVKQLIAHFDRLIDELDKQIDNHTHTHFDGKDQVAEQIKGIGSITTAT
                     LMAMLPELGRLSHKRIAGLAGIAPHPREGGETKFKSRCFGGRSAVRKALYMATVAATR
                     FEPLIRDFHQRPLSEGKPYKVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT          416 a          516 c          374 g          465 t
ORIGIN      
        1 tttgaaaacc ccattaaacc gtctttaacc catcgtcccg aacggtagat gtttcacccc
       61 tgcaaaccaa aaaaaagacc gtctgaatac agacggccaa agcctggtca cacatcactg
      121 cctaaaataa agctgcctgc tgtgccgcag gtcggctcat ttcattgata atcgtatatc
      181 ccgttcgtga agagatgccg tatttagggc atagcttcgt catcgccata agcccgctct
      241 ccttatcaat atcgcgcaat ttgacaaact cctgataaaa cctatggttt ctcaactgta
      301 ttaacgcctt gccgcaccgt gggacataca attcctcgcc accatatacc tgcaacagct
      361 catgtgtttt cacttcgccg atggcttcga ccaaaattgc caaacgctcg gtgtccacct
      421 tgcccttacc aaatttaaac cgcgccccgc caatcgcctt gaccagctgt tccgtcgctg
      481 ccagtccgat gacatccaca atgtccaaca cggtatccgg caataaatgt tcaacttttt
      541 cgaaccccat catccccacc cgctttttcc gttttcctgt tttccgcaat ctgcaacgca
      601 gcaaccagtt tatgtagctg cgtatcgtct aaatattcga ccttatcctt accaaacatc
      661 cgccgcgcca ttgcgtgtgc atagttccaa tgtttgccgc cgacggtcag cagggcttcg
      721 actttgtcca acattgccgc tgatgatgtc cgacgcagat gtggtttacc gtgtgggtta
      781 ccttttgctt taggcttaaa tccgtgcgac cgcatatcag cgacaacaga ctcaagttcg
      841 gaaacatcca tatccgcaca cgaccgcttg cccgtcacgc gctccaacac cgcgcgatag
      901 gtaccgtcgt ccaagcccag ctccttttgg gcaattttaa tttttgcaat caacgcccgg
      961 cgcatctcaa acccataaaa cacaatatat tgactaatta acgcatatta taccaataaa
     1021 atacaatata tagtattaag ccgatgtttt ttgcgaaacg gacagacatg aaaaagcaac
     1081 tgtatttttc accccgtcgg gcaaaaatac caaaactcaa atcaagccgt ccggataccg
     1141 ttttcggcgg tatcgttttc ggcaaaataa tcacgcatcc gggcattcga tatcgtcagc
     1201 agtttgcgca tacatgccgt aacggcaacc ttatacggct taccctcgga cagcgggcgt
     1261 tggtggaaat cccgaataag cggttcaaaa cgtgtcgctg ccacggtagc catatacagt
     1321 gctttacgca ccgcagacct tccgccaaag cagcggcttt tgaatttggt ttccccgccc
     1381 tccctcgggt gcggggcaat gccggccaaa cccgctatcc gtttgtgcga cagccgcccc
     1441 aattcgggca acatcgccat cagcgtagcc gtcgttatcg aaccgatgcc tttgatttgc
     1501 tccgccactt ggtctttgcc gtcaaaatgc gtgtgggtgt ggttgtcgat ttgtttgtcc
     1561 aattcgtcaa tcagccggtc aaaatgggca atcagttgtt tgacgcttcc gacttgcgtt
     1621 tcgtgaacct gatgcagacg gtttttctcg gcagtccgca tatccgccgg ttggttgcgg
     1681 cggttaacca aggcttccaa cacttcttcc gcttcggtgg gcggttggta gggcatggtt
     1741 tgcgaatctt ctttctgtgc cgtcatctgt g
//
LOCUS       DAXHTR010000202         1763 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.202, whole genome shotgun sequence.
ACCESSION   DAXHTR010000202 DAXHTR010000000
VERSION     DAXHTR010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1763
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.202"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..246
                     /locus_tag="ACFNML_001963"
     CDS             <1..246
                     /locus_tag="ACFNML_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6393273.1"
                     /translation="PRVIIEGSKMDALYVSAPFYFDDDFQVCYGGHYNTVFPLLVPLY
                     KQEAELVEKKGWNAFEQFLLDNEVDNLSDMNRKPFVW"
     gene            382..1050
                     /locus_tag="ACFNML_001964"
     CDS             382..1050
                     /locus_tag="ACFNML_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HFC6393274.1"
                     /translation="MVKTADGYKAIAHIQAGDRVFAKDETSGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITL
                     KQQPLKAYNLTVADWHTYFVKGNQAETEGVWVHNSCPPKRAPEYHAGTVSESNFLNVA
                     EKWLGENYKSYPNGRYVSQDGMRQVRYGHHETNSSTHHGHFESYDKPNGRVIENSVVT
                     IIQD"
     gene            1057..1326
                     /locus_tag="ACFNML_001965"
     CDS             1057..1326
                     /locus_tag="ACFNML_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393275.1"
                     /translation="MSVKELFKVILDKNKDFPIRTIHRTPMGVLPKPVALSIVQYEDD
                     QGFYLFYLDETGREQTDTYHDTLDSAFKQAEFEFGIRKEEWIQSS"
     gene            1486..>1763
                     /locus_tag="ACFNML_001966"
     CDS             1486..>1763
                     /locus_tag="ACFNML_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HFC6393276.1"
                     /translation="MIIVEHLEHYLGEIENGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRKPI"
BASE COUNT          582 a          321 c          355 g          505 t
ORIGIN      
        1 cctagggtaa ttatcgaagg tagcaaaatg gacgctctgt atgtttccgc cccattttat
       61 ttcgatgatg attttcaggt ctgttatggc ggacactaca atactgtttt tcctttgctt
      121 gtcccgttgt ataaacagga agcagaattg gtggaaaaaa agggttggaa tgcttttgag
      181 cagttcttgc tggataatga agttgacaac ctttcggata tgaataggaa accgtttgtt
      241 tggtaaagga ttttcttaaa tttgattaaa ggtcgtctga aattaagctt gtttttcaga
      301 cgacctttcc ttcatttgaa acagaatatt gagaactgag ttcttcaaaa atcctacacc
      361 tgctccttcc acggcagcac cttggtcaaa acggcagacg gctacaaagc cattgcccat
      421 attcaagccg gcgaccgcgt cttcgccaaa gatgaaacca gtggaaaaac tggatacaaa
      481 cctgttaccg cccgatacgg caatccgtac caggaaaccg tttacattga aatttcagac
      541 ggcatcggta acagccaaac cctaatttcc aaccgcatcc atccgtttta cagtcaagga
      601 aaatggatac aggcaggtcg tctgaaaaaa ggcgacaccc tgctttccga aagcggtgca
      661 aaacagacag ttcaaaacat taccctcaaa cagcagccgc tcaaagccta caatctgacc
      721 gttgccgatt ggcatactta cttcgttaag ggtaatcagg cggaaacgga aggggtttgg
      781 gttcataatt cttgtcctcc caaaagagct cccgaatacc atgcaggaac tgtatccgaa
      841 tctaattttt taaatgtagc tgagaaatgg ctaggagaaa actataagtc atatccaaat
      901 ggtcgatatg tatcacaaga tggaatgcga caggtacgat atggtcatca tgaaacaaat
      961 tcatctacac atcatggtca ttttgaatca tatgacaaac ccaatggacg tgtaatagaa
     1021 aatagtgtag taacaattat tcaggattaa aaaattatga gtgtaaaaga attattcaaa
     1081 gttatcttag ataaaaataa agattttcct attagaacga ttcatcgcac tccaatgggg
     1141 gtacttccca agcctgttgc tttaagtatt gttcaatatg aagatgacca aggattttat
     1201 ttattttatt tggatgagac cggtcgggaa caaacggata cttatcatga cacattagat
     1261 tcggcattta aacaggctga atttgaattt ggaattagaa aagaagaatg gatacaaagt
     1321 tcttaggtat caaacgtaag atgggttgaa aaaatcaaca atcaaaaggt cgtctgaaac
     1381 catgtttggt ttttcagacg acttttccgt taataaaaaa tagcgatatt ggaacccaaa
     1441 ccagcctacg gcttgttaga atagggatga aaagtttaag aaaaaatgat tatagttgaa
     1501 catttagagc attatttggg cgaaatcgaa aacggtataa aatgcttgga tcgacgttat
     1561 catctcagcg tttccgtatt cccatcacaa ccatataaag gggtaactac tttttctact
     1621 ttaggtttga atcgatatga cttgaactat aagagtaggt ttgaattaat ttttacctgt
     1681 tctgaagaat ggaataaaga aaatattgca gcttttctat caggagtagc tgaatatttg
     1741 attgacaata gaaaacctat ttt
//
LOCUS       DAXHTR010000203         1756 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.203, whole genome shotgun sequence.
ACCESSION   DAXHTR010000203 DAXHTR010000000
VERSION     DAXHTR010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1756
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.203"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(42..365)
                     /locus_tag="ACFNML_001967"
     CDS             complement(42..365)
                     /locus_tag="ACFNML_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFC6393277.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IIPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(353..592)
                     /locus_tag="ACFNML_001968"
     CDS             complement(353..592)
                     /locus_tag="ACFNML_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HFC6393278.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
     gene            complement(715..1512)
                     /gene="truA"
                     /locus_tag="ACFNML_001969"
     CDS             complement(715..1512)
                     /gene="truA"
                     /locus_tag="ACFNML_001969"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFC6393279.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTTAARPQQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGPMRRAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSAGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          387 a          415 c          513 g          441 t
ORIGIN      
        1 ttaatccact ataatgaaca caatccattc agactattca atcaggcaaa catctcctgc
       61 aatactgcaa acagtttttc agccgtactg ttgtctaaat ttccaagatg tttgaccaat
      121 ccggctttat ccacagccct aatctgttcg ggcaaaagca aaccgtcttt atcctgaaag
      181 cggacattga cgcggaacgg ggcaggacgg cttccgctcg tcatgggaat gatcagcaca
      241 gtcttgagat agttgtgtat ttcaggagga gagactacga cacaaggacg tgtctttttg
      301 atttcgcttc ctacggtcgg gtctaaggag accagataga ttccgccgcg tactaccata
      361 tccattcttt atccgcttcg ttttcaattt cggaaaaaaa atgctcctgc tcggtttcga
      421 caagcattgc ggcagcttct gcccatcccc tgcgaacggt aggacagctt aaaatgatat
      481 tgcccttttc aactgtaaca gccaagctgt ctactgcccc tatttgaccc aataatgatt
      541 tgggcagaat cacgccttgc gagtttccca ttttgcgtat gttgagaatc atatacgtac
      601 cctatttacc agtttatgta ataacaatgt tattaccttg atgaggtagt gtcaatattg
      661 aaaaagattg cctgacagtt tgtccgattt caaaatctcc gcgacaagaa tgttttaaag
      721 ccattcgggg atttgggggc ggatgatgcc gtatgcctcg ggatagtcga cgccggtcag
      781 gtaaagtccg tcgggcatga aggtcggcgg ggctttgagg cggctgcgtt cttgaatcag
      841 tgcggcgaag ccttcgacgc tgagtctgcc gctgccgaca tagacgagcg cgcccatgat
      901 gttgcgtacc atgtggtgca aaaaggcgtt gccgtgcaaa tcgaggcgga cgagtcctgc
      961 gctttgggta aggtcggcgc ggtagatggt tttgacgggg gattttgcct ggcattcggc
     1021 ggcgcggaag ctggagaagt cttgttcgcc gatcaataag gcggcagccc ggcgcatcgg
     1081 cccgatgtcg agtttgaggt gtgtccagcc tgccctgttt ttgagcaggg gggaacggac
     1141 gggggcggat tcgagcaggt agcggtaatg ccgtccgtat gcgtcgaagc gggcgtgaaa
     1201 ttcgggggcg acctgtcggg cgtgcaaaac ggcaatgcct tcgggcaggt gggcatttac
     1261 gccgcgcacc catgcctgtt ggggacgggc ggcagttgtg tcgaagtgga cgacttgggc
     1321 ggtggcatgc acgccggtgt cggtcctgcc ggcaacggtg gtggaaaccg cttcccctgc
     1381 tatttgggcg agcgcggttt ccaatgccgc ctgaacggtc ggtacgccgt cagcctgttt
     1441 ctgccagccg taaaagcggc tgccgtcata ggatagggtt attgcccagc gttgtttttg
     1501 tgcggtatcc atcggatttg ggattcggat aaatgttcag actgcattgt atcgcagatt
     1561 ttgcagggaa acggcaaatg cccggggcgc gcggcgttgt ttggggagtt gggggggggt
     1621 ggatgcagtt gctacgaatc gctatcctgt gaattcaccc tgtcaggagt gcccgaatcg
     1681 tcattcccgc gcaggcggga atctaggacg taaaatctaa agaaaccatt ttatagtgga
     1741 ttaacaaaaa ccagta
//
LOCUS       DAXHTR010000204         1740 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.204, whole genome shotgun sequence.
ACCESSION   DAXHTR010000204 DAXHTR010000000
VERSION     DAXHTR010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1740
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.204"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..>1740
                     /locus_tag="ACFNML_001970"
     CDS             <1..>1740
                     /locus_tag="ACFNML_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6393280.1"
                     /translation="AYDKWDYKQEGLTGAGAAIVVIIVTALTYGYGAAAAGSVTAAGS
                     STAAAATTTAAATTTVSTAAAMQTAALASLYSQAAVAIINNKGDVGKALKDLGTSDTV
                     KQIVTSALTAGALNQMGADIAQLNSKVRTELFSSTGNQTIANLGGRLATNLSNAGISA
                     GINTAVNGGSLKDNLGNAALGALVNSFQGEAASKIKTTFSDDYVAKQFAHALAGCVSG
                     LVQGKCKDGAIGAAVGEIVAESILGGRNPATLSDAEKHKVISYSKIIAGSVAALNGGD
                     VNTAANAAEVAVVNNALNFDSTPTNAKKHQPQKPDKTALEKIIQGIMPAHAAGAMTNP
                     QDKDAAIWISNIRNGITGPIVITSYGVYAAGWTAPLIGTAGKLAISTCMANPSGCTVM
                     VTQAAEAGAGIATGAVTVGNAWEAPVGALSKAKAAKQAAPKETINNLANLAKAEQQIL
                     FRIAQRDTQLDAWKTGFNNRVRKGAGLLDASNIPITINGKTIKPVQAISLKGAPVYSG
                     VSEQEIFALYRQMTGQNPNFRVLPDGRLANGIISTGEWAGTKIALRNFSKTENSTQAR
                     WTLDLQNPPSFIKG"
BASE COUNT          520 a          390 c          459 g          371 t
ORIGIN      
        1 gcctacgaca aatgggacta taaacaggaa ggcttgaccg gagccggtgc agcgattgtg
       61 gtgattattg taaccgctct aacttatgga tacggagcgg ctgcagcggg tagcgtaact
      121 gccgcaggaa gtagtacagc cgcagctgca acaacgacag cggcagcaac taccactgtt
      181 tctactgcag ctgccatgca aaccgcagct ttagcctcct tgtatagcca agcagctgta
      241 gccatcatca ataataaagg cgatgtaggc aaagcattaa aagatctcgg caccagtgat
      301 acggtcaagc agattgttac ttccgcactg acggcgggtg cattaaacca gatgggagca
      361 gatattgccc aattgaacag caaggtaaga accgaactgt tcagcagtac gggcaatcaa
      421 accattgcca accttggagg cagattggcc accaacctca gcaatgcagg tatctcagct
      481 ggtatcaata ccgccgttaa cggcggcagc ttgaaagaca acttaggcaa tgccgcattg
      541 ggagcattgg ttaatagctt ccaaggggaa gccgccagca aaatcaaaac aaccttcagc
      601 gacgattatg ttgccaaaca gttcgcccac gctttggcgg gttgtgttag cggactggta
      661 cagggaaaat gtaaagatgg ggcaattggc gcagcagttg gggaaatcgt agctgaatcc
      721 atacttggcg gcagaaaccc tgctacactc agcgatgcag aaaagcataa agttatcagt
      781 tactcgaaga ttattgcggg cagcgtggcg gcactcaacg gcggcgatgt gaatactgcg
      841 gcgaatgcgg ctgaggtagc tgtggtgaat aatgctttga attttgacag cacccctacc
      901 aatgcgaaaa agcatcaacc gcagaagccc gacaaaaccg cactggaaaa aattatccaa
      961 ggtattatgc ctgcacatgc agcaggtgcg atgactaatc cgcaggataa ggatgctgcc
     1021 atttggataa gcaatatccg taatggcatc acaggcccga ttgtgattac cagctatggg
     1081 gtttatgctg caggttggac agctccgctg atcggtacag cgggtaaatt agctatcagc
     1141 acctgcatgg ctaatccttc tggttgtact gtcatggtca ctcaggctgc cgaagcgggc
     1201 gcgggaatcg ccacgggtgc ggtaacggta ggcaacgctt gggaagcgcc tgtgggggcg
     1261 ttgtcgaaag cgaaggcggc taagcaagct gctcctaaag aaacaataaa caatttggca
     1321 aatttagcca aagcagaaca gcagatttta ttccgtattg cccaacgcga tacgcaactg
     1381 gatgcatgga agacgggatt taacaataga gtaaggaaag gagcaggctt gcttgatgca
     1441 agtaatattc cgataaccat taacggaaaa accatcaaac ctgtacaagc cataagctta
     1501 aagggagcac ccgtttacag cggcgtaagc gaacaggaga tttttgcgct ttatcggcag
     1561 atgactggcc agaatccgaa ttttagagtt ttgcctgacg gaagattagc aaatggcatt
     1621 atcagtactg gagaatgggc aggaacaaaa attgcattaa gaaatttttc aaaaacagag
     1681 aattcaactc aagcacgatg gacattagat ttgcagaatc ctccatcatt tattaaaggt
//
LOCUS       DAXHTR010000205         1732 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.205, whole genome shotgun sequence.
ACCESSION   DAXHTR010000205 DAXHTR010000000
VERSION     DAXHTR010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.205"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            170..1144
                     /locus_tag="ACFNML_001971"
     CDS             170..1144
                     /locus_tag="ACFNML_001971"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFC6393281.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALCGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDSFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVIMGSSEEVDYLNRLHSK"
     gene            complement(1303..1521)
                     /locus_tag="ACFNML_001972"
     CDS             complement(1303..1521)
                     /locus_tag="ACFNML_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFC6393282.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT          475 a          483 c          372 g          402 t
ORIGIN      
        1 ccactataag aataaatatc ccctcatccg cctgccgcgt tccgacttgc cccgcgccgt
       61 ttttttccac tacaatccca cgcgatatct ggcggaacgc accaaaggaa cgccccgcag
      121 caatctgccg cctttcagac ggcacaaaca tttccgaaac gagcaaacta tggacacact
      181 gacccgattc ctccccgaac acctgcaaca aaaccagctg cccgaagcac tctgcggcgt
      241 tttactgtcc gttgtttccg cctgcaccga aatcaacgcc aaagtccgcc tcggcgcact
      301 ggccggcgta ttgggtatgg cgggcacggg caatattcag ggcgaagacc aaaaaaaact
      361 ggatgttatc gccaacaaca tcatgattga cacactcaaa gccaaccctg ccgttgccgg
      421 tttggcaagc gaggaagaag actctttcgt aagtgccggt gaaaacggac gctatctcgt
      481 cctattcgat cctttagacg gatcgtccaa tattgatgtc aacatttccg tcggtacgat
      541 tttctccatc cttgaaaaac ccgaaggcgc attggcaacc gaatcattcc ttcaaacggg
      601 cagacagcag cttgccgctg gttacgttct ttacgggcct caaacccagc tcgtattcac
      661 attcggacac ggcgtatata tgttcacgct caatgccgaa aacgaatttg tgctgacaaa
      721 agaaaaaccg aaagtacccg aaagtaccaa agaatttgcc atcaatatgt ccaaccgccg
      781 ccactggctg ccccccgtcc aacaatacat cgacgagctc ttggcgggcg aaaccggcac
      841 gcgcggcaaa aactacaata tgcgctgggt agccagcatg gttgccgaaa tccaccgcat
      901 cctgatgcgc ggcggcgtgt tcatgtatcc gcaagacaaa cgcgaccccg ccaagcccgg
      961 caaactgcgc ctgatgtacg aagccaaccc tatgagcctg atactcgaac aggcgggcgc
     1021 atcggcaagc aacgcatatc aagccatgct cgacatacag cccgaaaacc tgcaccaacg
     1081 cgtcgccgtc attatgggca gcagcgaaga agtggattac ctcaaccgcc tgcattcaaa
     1141 ataaaaggca gcctgaccga taaagaaagg cggcagcgcg catccgacgc tgccgccgat
     1201 atttcgttta tagccttaaa aagatagaaa accacctttc cgaacggcaa aaatgccgtc
     1261 tgaaaagttt tcagacggca ttttaaaaat taaagcccga atttatttgc ccgctgcttt
     1321 ttgcaggatg gcgtacaact cgtctttcag tttcaatttg gcttttttca gctcatcgat
     1381 ggtttccgca ccgctggtaa ccggattgtt gaccagaccg gtaattttat cgtccagctc
     1441 gttgtgttcg tcgaacagac gggcgaagcg ggaattttcc tgtttcaatt tggaaatcaa
     1501 atcacggtat tctgggaaca tatcgctttc ctctcttggt ttacggttaa aaagtcaaat
     1561 tgaatcttta cattaattat aacaaagata atatttttat ccagtgtttt atcggatacg
     1621 gcaaaatttt cttttaacac cgccgtccgg ttgataaaaa cgttgacaat atcacgcttt
     1681 acgcataaat tttcagcctt tcctactgta tagtggatta acaaaaatca gg
//
LOCUS       DAXHTR010000206         1731 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.206, whole genome shotgun sequence.
ACCESSION   DAXHTR010000206 DAXHTR010000000
VERSION     DAXHTR010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.206"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(66..845)
                     /locus_tag="ACFNML_001973"
     CDS             complement(66..845)
                     /locus_tag="ACFNML_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFC6393283.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSIMKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            1348..1731
                     /locus_tag="ACFNML_001974"
                     /pseudo
     CDS             1348..1731
                     /locus_tag="ACFNML_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311098.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
BASE COUNT          435 a          519 c          354 g          423 t
ORIGIN      
        1 gtactggttt aaatttagtt cactataccg atacatccga atatgccaga ccgtctaaac
       61 ggtattcata cctcccaatc ctgcacgcgc ttcaaatcgt tttgcgccaa agtatccgcg
      121 tggcggttac ggctctgata ttccctgtct ttcatgatgc tgcttgccac ataattcaaa
      181 tgtgcctttg ccgcctccga agcctcgccc ggccgacggt tcgatatcgc ctcatacagc
      241 acgcggtgct gcgccatcag tttcggacgc ggatcttctt cctgattcag ataaataagg
      301 ctgctgcgcg tctgccggta cagcattttc aacaaaccgc ccgacaaatg gctgaacaac
      361 aaattgtgcg ccgcatccgc aatcgtctga tggaagctga catcggcttc gctctgatgt
      421 tctagattgc cgctttcgca cgcctcctca aacttttcaa gccaaaaccc aatccgcttc
      481 aaatcggcat ccgtccggcg ttctgccgcc aatgccgcca tacagccctc gatgtggcag
      541 ctgaaatcaa aaacatcctg ttcccaattg gaatgcttgc ccaaaagctc ctgccaactt
      601 tgcaaaaaat cctgctgcgg cttgaccgaa acataataac cgtctccctg cctcgcttcc
      661 aaaacctgac gggcgaccaa aacattcaat gccgacctga ccgacgggcg cgaaacgccg
      721 aactcttccg ccaaaacgcg ttcgggcgga atcttgcccc cttccgcgta aaccccttcc
      781 gcaatgcgct cctccaatac cgacaatacc tgatcgctga ttttctgagg ccttaccagt
      841 ttcatcactc ctcctttata aagattccct gcagaaccct tccgaaatat agtggattaa
      901 caaaaatcag gacaaggtga cgaagccgca gacagtacaa atagtacaga accgattcac
      961 ttggtgcttc agcaccttaa agaatcgttc tctttgagct aaggcgaggc aacgccgtac
     1021 tggtttttgt tcatccacta tacatcaaac atcaaattgg actgaccaat cagggcggat
     1081 tctaatgaca cgccgttccc gccgtcaacg gcatttacct cgcaccgccc ccgaaacaca
     1141 agaaaaaact acaccaaact acaatttttg ttcatgcaaa tatttgtttt gacaggattt
     1201 aaacgaaagc tccgattcaa atctgccgaa ccgcccaaaa atatattgac ctaaatatta
     1261 aagtttcgta aagtaatgca acgttgcttt aattggtttg accactattg ccgacgatta
     1321 gaaaaatatt ttcggagatg ttcaattatg gaaacttggg ttcaaaacta cacggcaatc
     1381 ggcggcagcc tgtatctgac tgccgccgcc gcactcttac ccatcgtctt tttctttgcc
     1441 gcgctgaccg tcctgaagct gaaaggctat caggcggggc tttatacgct gctgattgcg
     1501 cttgccgttg ccgtattcgg cttcgggatg ccggcggtat ggcggtttct tccctgccgc
     1561 agcagccgca ctgacccaac gcccctacgc cacgctggat ttgaccgcgc attacaaaat
     1621 cggcaaatcc acccgcatcg gtttggactt tgaaaacgtg ttcaacaaac gctaccgccc
     1681 tatgcccgac attcacgttt acggcacgcc gcgcagcctg accgcaaccg t
//
LOCUS       DAXHTR010000207         1730 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.207, whole genome shotgun sequence.
ACCESSION   DAXHTR010000207 DAXHTR010000000
VERSION     DAXHTR010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.207"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            356..1660
                     /locus_tag="ACFNML_001975"
     CDS             356..1660
                     /locus_tag="ACFNML_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HFC6393284.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDAAAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          355 a          432 c          510 g          433 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggattggat gcgatatttc catattcaaa caagggattt gtttcacgca cagggcggca
      241 catcggcaaa atccccgcgc cgccccggtc cggcagggct tgcgtccctc ccggacaagc
      301 cccgaccccg cctttccgaa agacgggctc aaccattaag gaaactttaa tcaaaatgaa
      361 aaaacacata tgggcggcat ctttgctgcc ggcatcccta tcggcagaac ctttaaactg
      421 gtggaagcct tattccgccg tcaattcggg cgataccgcc tgggtgatga ctgcggctgc
      481 cttggtactg ttgatgacgc ttcccgggct ggctttattc tacggcggta tggtgcggaa
      541 aaaaaacctg ctctcgacga tgatgcacag cttttccatc gcgacattgg tgggaatcct
      601 ttgggtcgcc gtcggctatt ctttagcgtt cacgccggga aatgccttta tcggcggttt
      661 ggggcgcgta tttttaagcg ggatgcagat agacgctgcc gcacagatgc tgaccgtgtc
      721 gcccaatgcg ccgactgttc ccgaaccggt atttatgttt tttcagatga cgtttgccat
      781 tatttcgacc gccattatta ccggcgcgtt tgccgaacgg atgaaatatt cggcaatgat
      841 gctgttttcg ggcatatggt ttttattggt ttatgtgccg ggcgcgcatt gggtctgggg
      901 cggcggcttt atgagcaagg gcggcgtatt ggattatgcc ggcggtacgg tggtgcacat
      961 caatgccggt atcgcgggac tcgtcgccgc cttggttttg ggcaggcgca taggctacgg
     1021 gcgcgaggcg atgcctccgc acaatatggc gatgacactg atcggcgcgg caatgttgtg
     1081 gttcggctgg ttcggcttta acgccggatc ggcgcttgcg gcagacgcgg cggcgggtat
     1141 ggcgatggcg gtaacgcagg tgtcggccgt attcggcgcg gcaggctggc ttgcctgcga
     1201 aaaaatagcg ggacacaaac cttccgcttt ggggctggct tccggcgcgg tttccggtct
     1261 ggtcggcatc acccctgccg ccggctttac cggcccgtct ggagcggccg ccatcggtat
     1321 attgactgcc gccgcgtgct ttgtgtccgt caccgtcgtc aaacacaaat tgcgttacga
     1381 tgattctttg gacgctttcg gcatacacgg attcggcggg ctggtgggcg gaatattgac
     1441 cggcatcttt ttcgacaacc gcattttcgg cggggatgcg gcagtttggc agcagttgtg
     1501 gatacaggta aaagacgggt tcgttatggc ggcatacagc gggctgatga gttgggcgat
     1561 tttgaaggtc gtggggaaaa tctgcggcgg cctgcgcgtc ggcaaggatg tcgaacgcga
     1621 aggtttggat ctgaatatcc acggagaacg cgtggaataa gggcggctat gccgtctgaa
     1681 gcctgaaaat cgggtttcag acggcatttt tcacgtttgc cgccgatgga
//
LOCUS       DAXHTR010000208         1670 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.208, whole genome shotgun sequence.
ACCESSION   DAXHTR010000208 DAXHTR010000000
VERSION     DAXHTR010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.208"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(195..1352)
                     /locus_tag="ACFNML_001976"
     CDS             complement(195..1352)
                     /locus_tag="ACFNML_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFC6393285.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNIQLQLTEPL
                     PNIQPQVTKRKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGGGASQWG
                     NRESFIGLAGEFGTLRAGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDDMSVSVR
                     YDSPEFSGFSGSVQFVPAQNSKSAYTPAHFVQNKQNQPPTLVPAVVGKPGSDVYYAGL
                     NYKNGGFAGNYAFKYAKHANVGRDAFELFLLGSGSDEAKGTDPLKNHQVHRLTGGYEE
                     GGLNLALAAQLDLSENADKTKNSTTEIAATASYRFGNAVPRISYAHGFDFIERGKKGE
                     NTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAASVGLRHKF"
BASE COUNT          431 a          424 c          398 g          417 t
ORIGIN      
        1 tatatgtgca aaccaagccg aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaag gcgagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgataaagtc gaaaccatgg gcatagctga tgcgtggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt gtcggcattt
      481 tcagacaaat ccaactgagc cgccaaggcg agattcaagc cgccttcctc atagccgccc
      541 gtcaggcggt gtacctgatg gtttttcaag ggatcggtac ctttggcttc atcactcccg
      601 ctgccgagca agaacaactc aaaagcatca cggcccacat tggcgtgttt cgcgtattta
      661 aaggcatagt tcccggcaaa accgccattt ttgtaattca gaccggcata atacacatcc
      721 gaccccggct tgccgacaac agccggaacg agagtaggcg gctgattttg cttattctga
      781 acaaaatgag ccggcgtata ggcggacttg ctgttttggg ccggaacgaa ttggacgctg
      841 ccgctaaaac cggaaaattc gggggaatcg tagcgtacgg aaaccgacat atcgtcgtgg
      901 cgtttgaaaa tacccaattg cgaagccaca tcattgttgc tgtcccaagg atcaatggct
      961 tggctggcat cgtcaaactg attcgcaacg cgaccggcgc gcagcgtgcc gaattcgcct
     1021 gccaagccga taaaggattc cctgttgccc cactgggacg cgccgccgcc ggcaacggat
     1081 acgtcttgct caagctgcca aacagccttc agcccttcgc ccaaatcctc actcccctta
     1141 aagccgataa acgagccgaa atcgctgatt ttcgtcctga tgcggctttt gcgcttagta
     1201 acctgaggtt gaatatttgg gagcggttcg gtcaactgca gctggatgtt cctgccttcc
     1261 acgccggctt tgatttcgcc gtacaggctg acatcggcaa cggccgcaag cggcagtgcg
     1321 gacaatacga gggcggtaag tttttttcgc atatcggctt ccttttgtaa atttgataaa
     1381 aacctaaaaa catcgggcaa acacccgata tgtcatcaat tatacccccc ccccccgcaa
     1441 aaaaccattt ttcagaacaa atatctgata aatgccgcaa cctttatttt aaaaatgatt
     1501 atattttgat ataaaacaat agcttatttt tcaaaaaacg ttgtgtttct acaacacaat
     1561 tcaagcgcag acctcgtgcg ggccgatgcg ctgctgcccg gatgcagtct cggcttttta
     1621 aaacgccata aaaaaacaca cgcggcactt tatagtggat taacaaaaac
//
LOCUS       DAXHTR010000209         1628 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.209, whole genome shotgun sequence.
ACCESSION   DAXHTR010000209 DAXHTR010000000
VERSION     DAXHTR010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.209"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..349)
                     /locus_tag="ACFNML_001977"
     CDS             complement(<1..349)
                     /locus_tag="ACFNML_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017147256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6393286.1"
                     /translation="MFNDNPVVYGKIKVQSWKARRDFNIVKQDLDFSCGAASVATLLN
                     NFYGQTLTEEEVLKKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIV
                     YLKYRKDDHFSVLR"
     gene            complement(482..672)
                     /locus_tag="ACFNML_001978"
                     /pseudo
     CDS             complement(482..672)
                     /locus_tag="ACFNML_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1427..>1628)
                     /locus_tag="ACFNML_001979"
     CDS             complement(1427..>1628)
                     /locus_tag="ACFNML_001979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393287.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT          528 a          378 c          238 g          484 t
ORIGIN      
        1 cgcgcaatac cgagaaatga tcatccttac ggtatttcag atacacgatg acggggattt
       61 tcaactgcgc gagctgctcg aaagacaggg catagccttt cgcttcaaaa cccaaatctg
      121 gcataatgcg ccgcatatcc tcaaacgacg cgcgcatctg ctccttatcc agctttttca
      181 acacttcttc ttccgtcagc gtttgcccgt aaaaattgtt caaaagcgtc gccaccgaag
      241 ccgccccgca ggaaaaatcc aaatcctgct ttacaatatt gaaatcccgc cgcgctttcc
      301 aactctgcac tttgattttt ccgtaaacaa caggattatc gttaaacatc ggtgcagcat
      361 tcagacgata agataaagaa acgacaacac acgccaacag aaaaacatat ttgaacttca
      421 tcatattgtc cacataaagg gcagcctgaa aatctttcag gctgcccttg tcaaattatt
      481 cctagctttc ggcttttttg gcaaaccaaa caatccgatt acccgcataa tactttccat
      541 ttattgaaat ccgacaagcc gcgcccaaaa atgccatgca ctgtcgattt ccgcagcaat
      601 ctttgtaccg ttttcttcaa attccaaata ttcacccaat aataaacttg aaacagaacg
      661 cgtgggcagc aagtgcccac cctacgctta ttcaaataat ttgattaaat aaagaataaa
      721 gaaaatgcat caggaacaaa attataatct gccacctgac tcacaccgct tttaaagtaa
      781 ggggcatcaa aatcaaaacc gcaaaaaaaa taatttttgc attgattttt aatagattta
      841 aaattcgaat atagtgtttc tccagattta aaaagtttat ctttatctat atatttgatg
      901 ctttccctat ccaaaataaa tattcaaaca ttaaaaaatc attacatgac caagccaaat
      961 cataaatttg attcaaatgg gtatcataaa gataaaaata ataaggtttg ggaacaggta
     1021 aaatatttaa tggaaaagga ggactaattt tcttaatatc tgatgactga tatccatatc
     1081 tttctatatt cttaaaaatt tcatctttct taagataaca agacattctg aagataacaa
     1141 gacattctga caatcacaac ctgttcatcg gatatattat ctatttgcat ggcgcgtata
     1201 acacgccatg cctgattaaa attagtctcc cttaccttag aatctattat cttttccaaa
     1261 gatgaaaatg ccccttatct tgaactctcc agttagaacc tggaagtttc gtacctctta
     1321 aatgtgtatt aatatttctt atagtttcat taaaatgcca cgcgctgccg atttcaacgg
     1381 taattttcgt accgctttct tcaaattcca ggtattcccc cattagctaa cgcaaagaag
     1441 cagacgccat ttcggcttcg ttatgataaa cccgccttcc gttgatatag acttccgccc
     1501 ctgtccgctc caaattccaa aaattccgta ctgaaatttc catatcccga tattgtgcag
     1561 accatgtttt ttcgaaggtt ttcataaaat ttcctatacc tgtccaatcg gcacatatca
     1621 attgcatt
//
LOCUS       DAXHTR010000210         1570 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.210, whole genome shotgun sequence.
ACCESSION   DAXHTR010000210 DAXHTR010000000
VERSION     DAXHTR010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.210"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            182..313
                     /locus_tag="ACFNML_001980"
     CDS             182..313
                     /locus_tag="ACFNML_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393288.1"
                     /translation="MNKTWKRQVFRHTALYTAILMFSHTGGGGGRRKRKRKRINTLL"
     gene            283..1570
                     /gene="pilC"
                     /locus_tag="ACFNML_001981"
                     /pseudo
     CDS             283..1570
                     /gene="pilC"
                     /locus_tag="ACFNML_001981"
                     /inference="COORDINATES: protein motif:HMM:NF040838.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PilC family type IV pilus tip adhesin"
BASE COUNT          473 a          377 c          384 g          336 t
ORIGIN      
        1 ccgttgggca tctgcagcgg tttgctaaaa aacgctttac cgtgatcagt gtgcaaagtt
       61 aaaatgggga agtaagcttt tcaatcagca attcggcggg cgcggaatcg ggcggtttac
      121 cgaaccccgg cgttcgcggc gcgccgcccc gtcccgtgaa ggcaaactca aggaataaaa
      181 gatgaataaa acttggaaac ggcaggtttt ccgccatacc gcgctttata ccgccatatt
      241 gatgttttcc cataccggcg gggggggggg caggcgcaag cgcaaacgca aacgcataaa
      301 tacgctattg taatgaacaa tcaaaagctg cctgaggtaa agtgggggaa cgactatagc
      361 tcattgacgg acaaaagcaa tgaacgcgaa gttacccata cgagtaaatt tgttgtagca
      421 aaaaagcaca ttagtttctc attcaataat accgatgaag ttgttgctga aaaaaaagat
      481 gctgtcgttt tcggcgcggc gacttatctg ccgccctacg gcaaggtttc cggttttgat
      541 accgctaagc tggccgagcg caaaaatgcc cttgatcaga ttggtacgac ccaccctggg
      601 ttgataggct acaactacca agatgtcact tgcgccagcg gaaactgtcc tgaacttagc
      661 tatagaaccc aatttacctt caataacggg ttgaaaagaa atggaaacgg cggcaggctg
      721 gatatatacg aagacaaaag ccgcgacagt tcgcccattt acaaattgaa ggattatcct
      781 tggttgggcg tgtctttcaa tttgggcggc gagagctcct tcaaaccaaa gagacaaggt
      841 tctttggtat cttcttttag cgaggatgtg acgcagcaaa atggtacaca agaccaatac
      901 aaaagcaaaa acctcgttta tacgacagac gattacaata gtaagggtaa taaaaaccat
      961 caggacaaac accacgccgt cgccttttat ctgaacgcca agctgcattt gctggataaa
     1021 aaacaaatca aaaatatcgc gcaaggcgta acagttgatt tgggtacctt gaaaacacgc
     1081 atcgagccga cggacgagtg gaaaaacagg caggggagct atttaaataa tggtacttgg
     1141 acgtatgaag agaaaggatc agtcagcgtc aaactcaaat tgccggaagt caaagcaggc
     1201 cgctgcatca ccacaccgaa ccccaatccc aaatccaaag ccccttcgcc ggcactgacc
     1261 gcccccgcgc tgtggttcgg agctgggcaa gatggtaagg cggagatgta ttccgcttcg
     1321 gtttccacct accccgacag ttcgagcagc cgcatctacc ttcaaaatct gaaaagaaaa
     1381 accgactcca acagacccgg ccgctattcc ctcgcagact tgagtgcgtc ggatattcaa
     1441 agtaaagagc cgactttcac aagtcggcaa acaatcatcc gcttggatgg tggcgtacgg
     1501 catatccaac tggatagaaa caatgaggtc actagtttta atggcgacaa cggaactttc
     1561 ggcattgtta
//
LOCUS       DAXHTR010000211         1548 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.211, whole genome shotgun sequence.
ACCESSION   DAXHTR010000211 DAXHTR010000000
VERSION     DAXHTR010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.211"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(189..1178)
                     /gene="porB"
                     /locus_tag="ACFNML_001982"
     CDS             complement(189..1178)
                     /gene="porB"
                     /locus_tag="ACFNML_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HFC6393289.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSVAHNGAQA
                     ASVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFMQYGGAYKRHQNVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVKDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADYTNDYDQVVVGAEYDFSKRTSALVSAGWLQEGKGESKFVSTAGGVGLRHKF"
BASE COUNT          383 a          350 c          397 g          418 t
ORIGIN      
        1 aggttttctt aaccctgagt cctagattcc cgcctgcgcg ggaatgacgg catattttga
       61 cattgaataa aaaagactaa aaacaggaaa aagccaaaca gaaaacagcg aaacagaaaa
      121 caggaaaaag ccaaacagaa aaaagcctgt ctggcgacag gctttttgtt gataccaatc
      181 tttgcagatt agaatttgtg gcgcagaccg acaccgccgg cagtcgatac gaatttgctt
      241 tcgcctttgc cttcttgcaa ccaaccggca gaaaccaagg cagaagtgcg tttggagaag
      301 tcgtattccg caccgacaac cacttggtcg taatcgttgg tgtagtctgc atcatcaaac
      361 gagcctttga agccgtgggc gtaagaaacg cggggcgtta cgttgccgaa gcggtatgcc
      421 aaggtagcgg caacttcggt ttgagagttg tgcgaattgt ctttaaccag tttcgcgtct
      481 tgttgctgta cggctacgga agcgtacagg gcatcattgt cgtaaccgct gaccaaacgg
      541 tgaatctggt atttctcaat cttcacgtta tccacattct gatgtctttt ataggcaccg
      601 ccatattgca tgaagaagcc gccgtttttg tagttgaagc cggcgtggta agattcgctg
      661 ttatgtctgc ctgcattgtc gttaagcgcg tattgtacgc tgccgctgag gccggcaaat
      721 tcgggagaat cgtagcgtac ggaaatgagg cgtgcttcgg gttcggcaat tttgtttaca
      781 cccaaatagt cgcttttgct atcccaagga ttgatgtcgc cggtgtcttt caggacgctg
      841 ttcaaacggc cgacgcgcaa tttaccgaag ccacctttca aaccgatgaa ggattggcgg
      901 ttgccccaac cggagtcagt accggcgata gatgcctttt gctcaacctg ccaaatggct
      961 ttcagaccgt taccgaggtc ttcttggcct ttgaagccga ttttcgaacc caaatcaacg
     1021 atgccggtac cggtttcaac gctagccgcc tgagctccat tgtgagctac ggagcgggaa
     1081 gtttctacgc cggctttgat ggtgccgtac agggtaacgt cagccattgc tgcaacagga
     1141 agggctgcca aagtcagggc aatcagggat tttttcattg ctgtattcct tttttggtta
     1201 agaaatttaa gccggccggg ctttccaagc cgcttagctt tgcatttacc gccgacgttt
     1261 tcggcgattc catgtctgaa actatagatg ttcctgtctg aaaaaacaaa cttctgcctg
     1321 tttttgtgtg gaattcgatg tgtattttga agggcggata gagatattat gggtattttt
     1381 tgttttataa catatggtta tttaaatttt ttaagatttg catttttaca acacttactc
     1441 gggagggtat tggagggcat tgcaaaccgg gggttataaa gacagcaaaa aacgcgcacg
     1501 tggtttcttt cggaacgggc ggatgcaaaa cccgcccctg cggcagga
//
LOCUS       DAXHTR010000212         1506 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.212, whole genome shotgun sequence.
ACCESSION   DAXHTR010000212 DAXHTR010000000
VERSION     DAXHTR010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1506
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.212"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..1079)
                     /gene="fic"
                     /locus_tag="ACFNML_001983"
     CDS             complement(<1..1079)
                     /gene="fic"
                     /locus_tag="ACFNML_001983"
                     /EC_number="2.7.7.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase Fic"
                     /protein_id="HFC6393290.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIAIGRDKLFIHPRLMELLR"
BASE COUNT          353 a          338 c          368 g          447 t
ORIGIN      
        1 cgcaatagtt ccattaggcg cggatgaatg aatagtttgt ccctgccgat ggcgatttct
       61 tgcagcacac ctatgtctga aagctctttc aggtacttag aggccgtctg ccgtttggct
      121 atccctgccg cttctaggtt ggcaatgcgt gtatatggct gctcaaacag aagatttacc
      181 agttcgtgcg tgtagattcc ttgtgcgtgt gtccgtatgt gttgccgtgt ctgctcgaac
      241 aggcggcgta tcgcatctat tttcgatacc gtccaatcgg cggtgtcagc tacgccgtct
      301 aagatgtaga ttatccagct ttcccagtcc tgccgttcgg ttacgcctaa aagcaggcgg
      361 taatagtccg ccctgttttc gatgatgtag cggctcaaat acaaaatagg caaatccaaa
      421 agcccttttt caatcaatag caggctgttc aatatgcgcc ccgtccgccc gttgccgtcc
      481 gtaaacggat ggatggcttc aaattggtaa tgtgccgccg ccatgatgat aagcgggtct
      541 aaatcgccgc tttcgtgaat aaaccgctcc caatttgcca gcttgccgcg tatggtttct
      601 tctccttcgg gcggggtata gacaacattt ccgctgttgc ctccttttag ggctgtgccg
      661 cctgttttgc ggatggccat ttcgtagggg tgcttgatgg cgttgcagac catgatggcg
      721 gtttgtgtgc ataaagggcg gctcgtcagt gattcatagc ctgcaaacag ggcggtgcgg
      781 tattgcaggg cttctttcgt ggcagggtct tgccgttccg tatccatttg cagggattga
      841 aacagcttgt ccgtggtggt tacgatgttt tcaatttccg aacttgcacg ggcttccata
      901 acaggaaggg tgttaatcag catggcttga ttcggtatca attctgccgc ctgctttaaa
      961 cgggcaaggg atgcacgggc ggctatacaa cgtttcagga tggttttgct ttcaatatcc
     1021 tgttttggcg gcaggggtgg taaatcgtta tagggaatat cgggtttcca gttgctcatg
     1081 tttaaaattt cggaaaattt aaagatgttt ccagtatata tttacgccgt gtatatatca
     1141 aggatatatg tttaaaaatt tggcttttgt aaatatatgg gcggtaaaac cgcccgcaaa
     1201 agtcaatttg catggggtta attcaaaatc cgcccttcat ctgcccgctt ccctaaaaaa
     1261 aaccgtatat atataactgt ctgcattcta tcgctccggc gacgataccc atatttccaa
     1321 gtttgtgtat caaaattgta tatcgggcat agactatttc ggcgaggacg aagatataga
     1381 tttccacgat tgaatacatg gaagccaagt acgtctatca acactatatt aaaacacagc
     1441 cttttttttg aggtttcggg taacttttaa accgtcattc ctacgaaaac agaaaatcaa
     1501 aaacag
//
LOCUS       DAXHTR010000213         1444 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.213, whole genome shotgun sequence.
ACCESSION   DAXHTR010000213 DAXHTR010000000
VERSION     DAXHTR010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.213"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            27..1364
                     /locus_tag="ACFNML_001984"
     CDS             27..1364
                     /locus_tag="ACFNML_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HFC6393291.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTVLVALPVQLAEFYIGRTGGKNAVDSFRALRPSTQWLWVGRMGVSACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIAATIRQDER
                     KRKKHTWLIGTAIFIVGIPSALSFGVWGEFKVFGKTIFDLWDYLISAVIMPVGALSVA
                     VFTAWIQDRQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT          263 a          340 c          389 g          452 t
ORIGIN      
        1 tatcttcaca tttcttaata aattttatga gtaaccatac ttcttggtcg tccaaaatcg
       61 gtttcgtgct tgctgcggca ggttcggcca tcggtttggg cgcgatttgg aaatttcctt
      121 atacggcagg caccaacggc ggcgcggtgt ttttcctgct gtttttgata tttaccgtct
      181 tggtcgccct gcccgtccag cttgccgagt tttatatcgg gcgcacaggc ggtaaaaatg
      241 ccgtcgattc cttcagggct ctgcgtccga gtacgcaatg gctttgggtc gggcgcatgg
      301 gcgtttccgc ctgctttatt ttgctgtcgt tttacagcgt ggtcggcgga tgggtattaa
      361 attatgtcgt ccacagtttc acgggggcgg ttcataccgg cgcggacttt gaagccttgt
      421 tcggcgcgac gatttccaat ccggcaggtt cgctgtccta tcaggcactg tttatgctga
      481 ttacggtttg ggtggtcaaa ggcggcattt cagacggcat tgaaaaggca aaccgttatc
      541 tgatgccggg gctgtttatc ctctttattg cgctggcaat ccgttcgctg acgctgccgg
      601 gtgcaatgga gggcgtgtct ttcctgctca aaccgaattg gtcgtacttt aaagccgata
      661 cgatgattac ggctttaggt caggcgtttt ttgccttgag catcggcgtt tccgctatga
      721 ttacctatgc ttcgtatttg ggaaaagatc aggatatgtt ccgctcgggg catacgatta
      781 tgtggatgaa cctcttggtt tcgctgttgg caggtttggt gatttttccg gcggtgttcg
      841 ccttcggttt tgaaccgagc caggggccgg gattgatttt tatcgtattg ccggcggtgt
      901 ttatgaaaat gccgttcggt acggttttgt ttgcggtatt tatgctgctg gtcgttttcg
      961 ccacgctgac ttcggcattt tcgatgttgg aaacggtcat cgccgcaacc atccgccaag
     1021 acgagcgcaa acgcaaaaaa catacttggc ttatcggcac ggctattttt attgtcggca
     1081 tcccgtccgc gctgtctttc ggcgtatggg gtgagtttaa ggttttcggc aaaaccattt
     1141 ttgatttgtg ggactacctt atttctgcag tcatcatgcc tgtcggcgca ttgagcgttg
     1201 ccgtatttac cgcttggatt caggacaggc agtctgtgtt aaaagatgcc ggcgcgggca
     1261 gcaccgtacc acgggcagtg ttgctgctgt ggctgaatac cttgcgctac ctcgccccga
     1321 ttgccattat tattgttttc atcaattctt tggacatcct ttaaaagcca tccaaacagc
     1381 aaaaatgccg tctgaaagcc tttcagacgg catttttgct tcaggttcag cctatttcgt
     1441 tcaa
//
LOCUS       DAXHTR010000214         1416 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.214, whole genome shotgun sequence.
ACCESSION   DAXHTR010000214 DAXHTR010000000
VERSION     DAXHTR010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.214"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(45..1229)
                     /gene="ubiM"
                     /locus_tag="ACFNML_001985"
     CDS             complement(45..1229)
                     /gene="ubiM"
                     /locus_tag="ACFNML_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HFC6393292.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTAVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGASSL
                     LEKYSNKHMLHAHPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
BASE COUNT          315 a          304 c          414 g          383 t
ORIGIN      
        1 caaaaatgcc gtctgaaacg tttcagacgg catttcggca gttttcaacc ggtcagttgt
       61 ttggtaatca gctttttcag cggcgggaag ttgttgccgg cacgcaatac caaaccgcgc
      121 agcagttttg ccggcgcggt ttcgttggta aacagtttca gcatcatatt ggttccgtga
      181 taaagcggat gggcgtgcag catatgtttg ttgctgtatt tttccaacaa ggaagatgcg
      241 ccgatgtcct gaccgcgttg ttcggcttcg agtatcagtt ttgccagaat atctgcgctg
      301 gaaagaccca agttgaagcc gtgtgccgtt acagggtgca taccgaccgc cgcatcgcca
      361 atcagcgcgc tgcgtttgcc gtagaaacgt ttggcaatca taccgacaag ggggtaatgg
      421 tggatgctgc tgaccaattc catatcgccg agcctgccct tgagctgttc ttttacgctt
      481 gccgccaatt cttcgggcga aaggttttga acgctgttga ttttatcggt atcgacggta
      541 atgacggtat tggtcaggtg ttcttccagc ggcagcagtg cgatggtgcg cccgtaatgg
      601 aagcattcgt aagcggtatg ttggttggaa agagtatgtt tcatgcggca gacgaacatg
      661 gttcggctgt agtcgtgcat atctgaagaa atgccgagtt gtcggcgggt ttgcgaaaaa
      721 cggctgtctg ccgccaaaag caggcgggcg gtcaggattt tgccgttttc taaaatgact
      781 tgtgcttcgt tgtcagatgt tttgacttct ttaacggccg tatcggtcag aatggtaatg
      841 ttgtcgagtt gggatacgac ttcgtaggca gcgcggcgga tattgtggtt ggaaatcaga
      901 tagcccaaac agtcggcagg ctcgccgcgc gcctcggtcg gttggggaaa gtggagctgg
      961 tagtcggaac gtccgttcag cactttggca tcgcgcaaag ggtagatttc gttttcggga
     1021 attttgtccc acatacccaa acgctgcatg atttcgcggg aaaaatgggt cagggcgatt
     1081 tcgcgtccgt catatggagg attttgcaga acagtcagtg ggctgcgttc gatcagggta
     1141 actttcaaac cgctgccggc aagttcggct gcaaaactta aacccgccgg gcctgcgccg
     1201 acgacgagga tgtcgctgtg taaactcata aaatatcctt tgcatagacg gatgccgatg
     1261 atttcagacg gtatttgtaa gggtttgaat gccgtttgaa ctatctgtaa cagataggcg
     1321 attatatcaa aacccactgt tgaagaaata tgcaggggag ggtgtatgcg gatttttact
     1381 ttcagcttaa tgtgtatcaa atcgggtgtg gggtat
//
LOCUS       DAXHTR010000215         1402 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.215, whole genome shotgun sequence.
ACCESSION   DAXHTR010000215 DAXHTR010000000
VERSION     DAXHTR010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.215"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            286..1209
                     /gene="lpxC"
                     /locus_tag="ACFNML_001986"
     CDS             286..1209
                     /gene="lpxC"
                     /locus_tag="ACFNML_001986"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFC6393293.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVHFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDILNPEGLRYPDEFVRHKILDAIGDLYIVGHPIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRTCG"
BASE COUNT          351 a          358 c          328 g          365 t
ORIGIN      
        1 attttaggtt gctgtttttg gttttctgtt ttggttttat gtccttgtgg gaatgacggt
       61 tcacgcgaaa atgccgtccg aaaggttttc ggacggcatt ttttgcgttt ttcaggaggg
      121 ggcgggttcg tcgccttgtc ttgatttttg ttaatccact atacattttt atgcggctta
      181 cgtattgaaa acggatacat tggcaaatac tatggaaaaa catagcttgt tttccggttt
      241 tatcgtatta ttccaaaact taccccattg ataagggagc ggtttatgct gcaaagaact
      301 ttggcgaaat ccatcagcgt taccggagtc gggctgcatt cgggcgaacg cgtcgcgctg
      361 accctgcacc ccgcgcctga aaacagcggg atttccttcc gacgcaccga tttggacggc
      421 gagatgggcg aacaaatcaa actgacccct tatttgatca acgatacccg cctttcctcc
      481 accatcgtta ccgacaaagg cgtgcgcgtc ggcacgatcg aacacattat gtccgcgctg
      541 tccgcctacg gtatcgacaa cgcgctgatt gagctgaacg cgcccgaaat cccgattatg
      601 gacggctcca gcctgccgtt tatttacctt ttgcaagatg cgggcgtggt cgatcaaaag
      661 gcgcaaaagc gttttttgaa aatcctcaag cctgtcgaaa tcaaagaagc gggcaaatgg
      721 gtgcacttta cgccgtatga cggctttaag gtaacgctga ccatcgaatt cgaccatccg
      781 gttttcaacc gcagctcgcc cacttttgaa attgatttcg ccggcaaatc ctacatcgac
      841 gaaatcgcgc gcgcgcgcac tttcggcttt atgcacgaag tggaaatgat gcgcgcccac
      901 aatctgggtt tgggcggcaa tttgaacaac gccatcgtga ttgacgacac ggatatcctg
      961 aatcctgaag gcttgcgcta tcccgatgag tttgtgcgcc acaaaatcct tgatgccatc
     1021 ggtgatttgt atatcgtcgg acatccgatt gtcggcgcat ttgaaggcta caaatcggga
     1081 cacgccatca acaacgcact cttgcgcgcg gttttggcag acgaaacggc ttacgaccgg
     1141 gtggaatttg ccgacagcga tgatttgccc gacgcatttc acgagctgaa catcagaact
     1201 tgtggataac gctttctatt ttttccggta aattcctaaa acttaaaatt tcatcattcc
     1261 tacaaggaca aaaaaccaaa acagaaacct aaaattcgtc attcccgcga acctacatcc
     1321 cgtcattccc acgaaagtgg gaatccagaa cgcaaaatct aaagaaaccg ctttcccgat
     1381 aagtttccgt accgacagac ct
//
LOCUS       DAXHTR010000216         1361 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.216, whole genome shotgun sequence.
ACCESSION   DAXHTR010000216 DAXHTR010000000
VERSION     DAXHTR010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1361
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.216"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..890
                     /locus_tag="ACFNML_001987"
     CDS             <1..890
                     /locus_tag="ACFNML_001987"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFC6393294.1"
                     /translation="TAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKA
                     NLPKDAQISESELSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAQNPALKNLSP
                     AQRRELADNIIAEKVRQQAVMQNSRVGEAEVDRFIEQAQKQGITLPEGAPLRQYRAQH
                     ILIKADSENAAVGAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVM
                     VPAFEEAVHALKPGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAE
                     QATVNLLRDLHSGAYVDIR"
BASE COUNT          360 a          412 c          358 g          231 t
ORIGIN      
        1 ccacggcgca aaaagaagcc gcacccgcac aacagcaggg cggtatccgc ttttcagacg
       61 gcattgccgc cgttgccgac aacgaagtca tcacgcgccg ccggcttgcc gaagccgttg
      121 ccgaagccaa agccaacctg cccaaagacg cgcagataag cgaatccgag ctgtcccgac
      181 aggtgctgat gcagcttgtc aaccaatccc tgatcgtaca ggcgggcaaa cgccgcaaca
      241 ttcaggcaag cgaagcggaa atcgatgccg tcgtcgcaca aaatcccgcc ctcaaaaacc
      301 tcagccccgc ccaacgccgc gagcttgccg acaacatcat tgccgaaaaa gtccgccagc
      361 aggcagtgat gcaaaacagc cgggtcggcg aggcagaggt cgaccgcttt atcgagcagg
      421 cgcaaaaaca aggcatcacc ctgcccgaag gcgcaccgtt gcgccaatac cgcgcccaac
      481 acatcctgat taaggccgac agcgaaaacg ccgccgtcgg cgcggaaagc accatccgca
      541 aaatctacgg agaggcccgc agcggcacag acttttccag cctagcgcgc caatattcgc
      601 aagacgcgag cgcgggcaac ggcggagatt tgggctggtt tgccgacggc gtgatggttc
      661 ccgcctttga agaagccgtc cacgcgctca aacccggaca ggtcggcgcg cccgtccgca
      721 cccaattcgg ctggcatatc atcaaattga acgaagtgcg cgatgccggc acacctcagg
      781 aacgtatccg caattccgtg cggcaataca tcttccaaca aaaagccgaa caggcaaccg
      841 tcaacctgtt gcgtgacctg cattccggcg cgtatgtcga catccgctaa ggcggtttga
      901 agcaaaaagc cataccgatc ggcaaaaatc cgggcggtat ggctttttgg atttcgagtt
      961 acttttacac cgtcattcat cattcccgcg taggcgggaa tctagcaacg aaaagtaaca
     1021 ggaatttatc gggaaaacag aaacctctat gccgtcattc ccgcgcaggc gggaatctag
     1081 gacgtagaat ctaaagaaac cgttttatcc gataagtttc tgtaccgaag aatctggatt
     1141 cccgctttcg cgggaatgac ggcgcataag ttcccgtgcg gacagaccta gattcccaca
     1201 tgcgtgggaa tgacgattca gaagttgccc gaaaccaaaa aactgaagcc gaacggtctg
     1261 gattcccgct tttacgggaa tgacggcgca taagttcccg tgcggacaga cctagattcc
     1321 cacatgcgtg ggaatgacga ttcagaagtt gcccgaaatc a
//
LOCUS       DAXHTR010000217         1327 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.217, whole genome shotgun sequence.
ACCESSION   DAXHTR010000217 DAXHTR010000000
VERSION     DAXHTR010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.217"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(269..532)
                     /locus_tag="ACFNML_001988"
     CDS             complement(269..532)
                     /locus_tag="ACFNML_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393295.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(551..>1327)
                     /locus_tag="ACFNML_001989"
     CDS             complement(551..>1327)
                     /locus_tag="ACFNML_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HFC6393296.1"
                     /translation="EIVGEALVKNTDFSGMTASEIEKAKANITAYAKLVAGATVGVTG
                     GNVDVAANASETAVKNNALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDA
                     AAAAVPFVPAGATKISRGGAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTT
                     PAGLDYGLDAKHGNRIKHVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLP
                     NDSSVYLVDMKKPIGTKGETKVRIVVQKGTNKIISAYPQK"
BASE COUNT          368 a          303 c          235 g          421 t
ORIGIN      
        1 gtatccgggc aggattaagg gggtgtttgg gtaaaattag gcggtatttg gggcgaaaac
       61 agccgaaaac ctgtgttggg atttcggttg tcgtgaggga aaggaatttt gcaaaggtct
      121 caattggtgt tggtgaatgc cttcggctgc cagcagttgc attgaggccg tctgaaaaac
      181 ggatattcag acggcctatt gctactttaa ataatatttg ggtttaaaat atcatcgtat
      241 ttcccaaatt cggggaacag aaaatcaatt atttaaattt ttcatcctca taatatgggt
      301 gatcaaaaag ctctccttcc cacatattta tttcttctat gcctttatct ttcataaact
      361 catgataatc ataaaaatct tttccatatt gtccgtaaaa aattttcata tccccaagcc
      421 acatagcata acattcatcg cataaaatga cctccccaga gaaatattta ggctttgcat
      481 gataaaggta gccttgctca catcttgggc aaattttaat ttctttatcc ataagagatt
      541 cctttcttaa ttatttctga ggatatgcag aaatgatttt atttgttcct ttttgcacaa
      601 caatccgcac ttttgtttct ccttttgttc caataggttt cttcatatct acaagatata
      661 cggatgaatc atttggcaaa ggatttcctt tcattctcca agcttcatca accaaaggca
      721 aaacttcttt acgggacaca ttaaaaacag aatgaacagg tttgtttgga tttggaattg
      781 tatgggctaa aacatgttta atcctattac catgcttagc atctaaccca taatctaacc
      841 ccgccggtgt tgtccaaacc ttattaacct tatcataagt aagcttgatt ccggtctgct
      901 tctgaatttc ctgtatggct ttagccgtat caactgcttc gtctcccgcc ttcagaacat
      961 aagccccgcc tcggctgatt ttagtcgcac ctgccggaac aaagggaacg gcagctgcgg
     1021 cggcatccac accaaaatca atcgccgctt ctcgagccat ctgcttatcg ccaatagatt
     1081 tggcgtaaat ccatttaccg ccgtcccata cgaggttgcc gatatcccaa ataatatcta
     1141 atgcattatt tttaacagct gtttcggaag catttgccgc cacatcaaca ttgcctcctg
     1201 taacacctac agtcgctccg gctaccaatt ttgcatacgc agtaatattc gctttagctt
     1261 tttcaatttc agaagcagtc ataccgctga aatcggtatt cttaaccaaa gcctccccga
     1321 caatctc
//
LOCUS       DAXHTR010000218         1284 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.218, whole genome shotgun sequence.
ACCESSION   DAXHTR010000218 DAXHTR010000000
VERSION     DAXHTR010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1284
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.218"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..346)
                     /locus_tag="ACFNML_001990"
     CDS             complement(<1..346)
                     /locus_tag="ACFNML_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393297.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHII"
     gene            complement(910..1113)
                     /locus_tag="ACFNML_001991"
     CDS             complement(910..1113)
                     /locus_tag="ACFNML_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393298.1"
                     /translation="MLSLEIQQEGFTIGGGLVLGGCAGAHLARKEPLILTGKTGAGAS
                     AIANASIGYQWTVNLSEPKEGAK"
BASE COUNT          456 a          238 c          155 g          435 t
ORIGIN      
        1 taatgatatg atttttattt aaaataattt ttccatataa aatttccggt ttgtaatagc
       61 aagagtctga agcaaatcct tgcccgtcaa tataagaaaa ataaatgtta tcatcctcct
      121 tataatattc taaaaccaat ccggatatta ttttatcaaa attcccttca tttccatctt
      181 tcatgactaa aatataatta gcaggccaac ctttatcgta aacatattca aactgatagt
      241 tcccgaaact ctcgatatcc gaactaaaaa agaagaaaag caaattaata gccaatacaa
      301 aaacaaataa gaacaaaaat agcaaaattt tcaacttagt taacaatatt tacctctcct
      361 ttaaattcaa tcctgaaagg taccccttac cctgggctac caattatagt ttccatattt
      421 ctaaatattg tttttacatt acttttttct ccccccaaag gaatgcattt taaaatcatg
      481 cttttcaggt gctaatcgat acttaccatt aacatcttta atcacagata tatttccatg
      541 tatagcccaa cgtgaaaaat ctgagtatta tatacagtta taccttactg aaacaaagct
      601 agctgtacat atctgtacca tatttttact acaaacaaaa aatatttata attattaagg
      661 ataagataaa aacccccaac gctagattaa ttcttaataa tttcaaataa aaaatatttg
      721 agattttttc ttttccaaat aatatattga aatatttaaa gttaaggtag cttgatttaa
      781 gtagaaaatc aaaaaaacca ttaattaatt tttcttcttt aaatatttta tatgatatta
      841 taaaattaaa taacattgcg aaaacatatg aaatcatcaa aataccaaat atatagtggg
      901 aatgcattat tatttagctc cttcttttgg ctctgacaaa ttgacagtcc attgatatcc
      961 aatgcttgca tttgcaattg ctgacgcacc tgcccctgtt ttccctgtta gtatcaatgg
     1021 ttcttttctt gcaagatgtg cacctgcaca accacctaat actaaaccac cacctattgt
     1081 aaatccttcc tgttgtatct ctaatgataa cattttcttc ttgtgccgcc cttaatacag
     1141 attgtggaat tggattgacc tgagcaggaa cttctaaaat aagttttcct tcaagctgtc
     1201 ttcctcttag cgaagcaggt gtacttggaa cattggaaat gacaaagtga gtgcgcaaag
     1261 ccacctgaag gcgatgtgtt tgaa
//
LOCUS       DAXHTR010000219         1269 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.219, whole genome shotgun sequence.
ACCESSION   DAXHTR010000219 DAXHTR010000000
VERSION     DAXHTR010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.219"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            41..373
                     /locus_tag="ACFNML_001992"
     CDS             41..373
                     /locus_tag="ACFNML_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393299.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGTESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GEACACGH"
     gene            705..1214
                     /locus_tag="ACFNML_001993"
     CDS             705..1214
                     /locus_tag="ACFNML_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HFC6393300.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
BASE COUNT          341 a          241 c          301 g          386 t
ORIGIN      
        1 accttgtttt gacggaaaac catcatataa aggaacactt atgcagattt tatcttttca
       61 accggacatt gcggaacgta tgctggaagg tacggaaggc gagtcggtca acgaaaacgc
      121 acaattcgtc cgtacggaca acggctattg gattgcgtgg catgaaggcg tagcggcact
      181 gcttgcgccc gatacgccgc cgggcattcc ctgtttttgg gtggaaggca cggaaagcct
      241 tgaagagttg tgcgtcatgg tggaacgcgg cgagtttgac gaagtggaag agtttgacgg
      301 cgatgacgac gaatggctcg aaacggcaca gggttgcggg caccacggcg aagcttgcgc
      361 ctgcggacat taaaggcatt gcaggcttgc cgcaaggggt gcaaggcttt gccgtttttt
      421 aaataatcgg tggattgcat ctgtttgcaa tgggcggaac gcttcccttt gtattttatt
      481 gaataaaaaa acttatcttg gtattataat taaggcatca tcaattattt tgggatggca
      541 ataaacgcaa agcattgatt tgcgccgatt gcagacttat tatagcaggt tgcggcgcgg
      601 acttaatggg aattgtttat agagcatgat ggtctgaaac atcattaatt aaaaatacca
      661 ttaatccgat ttatatttat ttcaatttca atggaaaaac atcaatgaca atgattttaa
      721 gcattttaag cctgtttttt atcatcagac tgttattttt agccgtctct attaaacatg
      781 aaaaagcctt gattgccaaa ggggcgaaac aatacggaaa aaccaattcc acgctgcttg
      841 cggcagttca tacgctttat tatttggcgt gttttgtttg ggtatggctt tctgacactg
      901 cttttaatgg catatccttg attggtacgc tgacggtgat ggcttcgttt gtgatattgt
      961 cattgattat taagcagttg ggggagattt ggacggttaa aatctatatt ttaccaaatc
     1021 atcaaattaa tcgttcgtgg ttgtttaaaa cattccgcca ccccaattat tttttaaaca
     1081 tcatacccga actgattggc atcgccttat tatgtcaagc gtggtatgtt ttattgattg
     1141 gcctgccgat ttatttgctg gtcttattta agcgtatccg acaagaagaa caggcgatgg
     1201 caacactttt ttaacccgtt tcatcaatta tagcggatta acaaaaacca gtacggcgtt
     1261 gcctcgcct
//
LOCUS       DAXHTR010000220         1265 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.220, whole genome shotgun sequence.
ACCESSION   DAXHTR010000220 DAXHTR010000000
VERSION     DAXHTR010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1265
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.220"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(333..743)
                     /locus_tag="ACFNML_001994"
     CDS             complement(333..743)
                     /locus_tag="ACFNML_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393301.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIKGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(747..>1265)
                     /locus_tag="ACFNML_001995"
     CDS             complement(747..>1265)
                     /locus_tag="ACFNML_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HFC6393302.1"
                     /translation="KWDWVKNTGYKTPAARPMQTLDGEMAGGNKPAKQATPNGKAGVS
                     TQQSLQNSLVPQKNYTHTTYNGGSAITDGKYITDPLSMVRHKPDASSLDKIDSKSLFK
                     SDVDAEKATLDAARYADKHNLWDSQNKAKVPTNDVVGYTDGKPTHIINIYRRKPNKDG
                     FRPVHGTPGKER"
BASE COUNT          375 a          235 c          203 g          452 t
ORIGIN      
        1 ttgtatccga caaaaatatt tgacgcgtct atcgtttccg aaatgccgct gttgaaaaac
       61 gtcggattcg agaatccgac ctacggttgc tgaacaatgt tttgtagaac ttgctcaaaa
      121 tccatgtttc gccattttcc tttatatata cctaatgcag cacgggttac gatttcgttt
      181 aagtcataaa tagccattta cgtcctctgc taattttcag cgagtagatt gggttaccaa
      241 acccaacaaa aaagcagcct gaaaaatttc aggatgcttg ttttatgttt tttatcaatg
      301 aattaggttt taatccagcc tgcgcttgct agttaaacag tatttacata aaaatcttgc
      361 actgaacaaa caagcgtatc atgttcatca tgaaatgatg ggcataacgt taatctacaa
      421 atgtttacat ttaaagatat tttttcagtt tcaatatttt taatttgtaa ggttaataca
      481 tgaggatatt tgtgatatag ctgatttttt aattgttcgt tgtctaatat agcaaaaggt
      541 aattttgcac acaaataacc aataactttg tttttatgaa aaaaaactat ccaattttca
      601 attccttcat cccaatcaaa agtaaaatcc gttcctttta tgataaattt agcacaagat
      661 attaaatcta acttaaccca agtggattta tacagactca atgctgatga agttataata
      721 ttatctaaat tataaatttt cataaatcaa cgttccttac ctggtgttcc atgcacaggt
      781 ctaaacccat ctttattcgg ctttctacga taaatgttaa ttatatgtgt cggttttccg
      841 tctgtatagc caactacgtc gttcgttgga acttttgcct tattttgact atcccataaa
      901 ttatgtttat ctgcatatct agcagcatct aatgtagctt tttccgcatc aacatctgat
      961 ttaaataaac ttttgctatc tatcttgtct agactagatg catcaggttt gtgtcgtacc
     1021 atactgagag ggtcagtaat atatttacca tctgtaatag ctgatccacc attataggta
     1081 gtatgcgtat aatttttttg tggaacgaga ctattttgca aagattgttg tgtagaaacg
     1141 ccagctttgc cattaggagt agcttgcttc gctggcttat tcccaccggc catttcccca
     1201 tctaaagtct gcataggtcg ggcagcaggt gttttatagc cggtattttt aacccaatcc
     1261 cattt
//
LOCUS       DAXHTR010000221         1253 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.221, whole genome shotgun sequence.
ACCESSION   DAXHTR010000221 DAXHTR010000000
VERSION     DAXHTR010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.221"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            236..1189
                     /locus_tag="ACFNML_001996"
     CDS             236..1189
                     /locus_tag="ACFNML_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFC6393303.1"
                     /translation="MGFLIGLSRNLTRFTALVILLASAVAFIGPASFAWVKGDVQVLV
                     LGIIMLGMGMTLGREDYRILAQRPLDVFIGAAAQYTVMPLLAIGIAKLFDLSPGLTLG
                     LVLVGTCPGGVSSNIMSFLAKGDVAFSVGMTTVSTVLAPAVTPLWMTCLVGQTVDMDG
                     WGMFRFMLLVTLLPVVLGSAANILLHGKHWFENVRAVMPGVAVLAFACIVGGVASVHG
                     HRFAESALVMVLAIAVHNIAGYVSGYYAAALFGMDTAKKRTLAIEVGVQNAGLATGLS
                     AKFFPGNPESAVAAAVACVWHSVSGTVLGNLFALWDKRRDA"
BASE COUNT          200 a          272 c          432 g          349 t
ORIGIN      
        1 aaaccggatg attcgggcta aataattcct gattctttgg atatagccaa cggtatgcct
       61 gccgatgccg tcagaaaggg cttgatgggc gtttgcggtt cgggttaata tttgtgctgt
      121 atctgttaat tccggttagt aaaatggggc ggggacgatg agtggccgtc ggtcggtttt
      181 gcacgggcgg taataatgtc cggcgcaagg tgcgccgttt cgggaggggt gtgtgatggg
      241 ttttctgatt ggtttgagcc gcaacctgac gcggtttacg gctttggtca ttttgcttgc
      301 gtcggcggtt gcgtttatcg gaccggcttc gtttgcgtgg gttaagggcg atgttcaggt
      361 cttggtgctg ggcatcatta tgctgggtat ggggatgact ttgggcaggg aggactaccg
      421 gattttggcg cagcgtcctt tggatgtgtt tatcggtgcg gcggcgcaat ataccgtgat
      481 gccgttgctc gccatcggta ttgccaagct gttcgacctt tcgccggggc tgactttggg
      541 gctggtgttg gtggggacgt gtccgggcgg cgtgtcgtcc aacattatga gtttccttgc
      601 caaaggcgat gtggcgtttt cagtcggtat gacgacggtg tctacggtgc tggctccggc
      661 cgttacgcct ttgtggatga cctgcctggt cggtcagacg gtggatatgg acggttgggg
      721 gatgttccgg tttatgctgc tggtcacgct tttgccggtg gtgctgggtt cggcggcaaa
      781 tatcctgctg cacggaaaac attggtttga aaatgtccgt gccgttatgc cgggcgtggc
      841 ggtattggca tttgcctgca ttgtcggcgg cgtggcttcg gttcacggac accgttttgc
      901 cgaatcggcg ttggtaatgg tgttggcgat tgcggtacac aatatcgccg gctatgtgtc
      961 aggctattac gccgccgcgc tgtttggtat ggatacggca aaaaaacgca ccttggcaat
     1021 cgaagtcggc gtgcagaatg ccggtttggc aacaggattg agcgcgaaat ttttcccagg
     1081 caaccccgaa tcggcggttg cggcggcggt cgcctgcgtg tggcattcgg tttcggggac
     1141 ggtattgggc aacttgttcg ctctgtggga caagcgtcgg gacgcttagt cgggcgtgtc
     1201 cgaaccgtcc cacgatatat agtggattaa ctttaaaccg gtacggcgtt gcc
//
LOCUS       DAXHTR010000222         1101 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.222, whole genome shotgun sequence.
ACCESSION   DAXHTR010000222 DAXHTR010000000
VERSION     DAXHTR010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.222"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..>1101
                     /gene="tuf"
                     /locus_tag="ACFNML_001997"
     CDS             <1..>1101
                     /gene="tuf"
                     /locus_tag="ACFNML_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012081251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HFC6393304.1"
                     /translation="RSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQID
                     NAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA
                     DGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDD
                     CPIVQGSALKALEGDAAYEEKIFELAAALDSYIPTPERAVDKPFLLPIEDVFSISGRG
                     TVVTGRVERGIIHVGDEIEIVGLKETQKTTCTGVEMFRKLLDEGQAGDNVGVLLRGTK
                     REDVERGQVLAKPGTITPHTKFKAEVYVLSKEEGGRHTPFFANYRPQFYFRTTDVTGA
                     VTLEEGVEMVMPGENVTITVELIAPIAMEEGLR"
BASE COUNT          293 a          309 c          283 g          216 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggtggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gtcaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ctgcactgaa agccttggaa ggcgatgccg cttatgaaga
      541 gaaaatcttc gaactggctg ccgcattgga cagctacatc ccgactcccg agcgtgccgt
      601 ggacaaaccg ttcctgctgc ctatcgaaga cgtgttctcc atttccggcc gaggtacagt
      661 agtaaccggc cgtgtagagc gcggtatcat ccacgttggt gacgagatcg aaatcgtcgg
      721 tctgaaagaa acccaaaaaa ccacctgtac cggtgttgaa atgttccgca aactgctgga
      781 cgaaggtcag gcgggcgaca acgtaggcgt attgctgcgc ggtaccaaac gtgaagacgt
      841 ggaacgcggt caggtattgg ccaaaccggg tactatcact cctcacacca aattcaaagc
      901 agaagtgtac gtactgagca aagaagaggg cggccgccat actccgttct tcgccaacta
      961 ccgtccgcaa ttctacttcc gtaccaccga cgtaaccggc gcggttactt tggaagaagg
     1021 tgtggaaatg gtaatgccgg gtgagaacgt aaccattacc gtagaactga ttgcgcctat
     1081 cgctatggaa gaaggcctgc g
//
LOCUS       DAXHTR010000223         1050 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.223, whole genome shotgun sequence.
ACCESSION   DAXHTR010000223 DAXHTR010000000
VERSION     DAXHTR010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.223"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            18..602
                     /gene="rnhB"
                     /locus_tag="ACFNML_001998"
     CDS             18..602
                     /gene="rnhB"
                     /locus_tag="ACFNML_001998"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFC6393305.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPEIFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAVAWHVAAASPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLNIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQALF"
     gene            complement(863..>1050)
                     /locus_tag="ACFNML_001999"
     CDS             complement(863..>1050)
                     /locus_tag="ACFNML_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393306.1"
                     /translation="IFVNPLYRHSRKNKKIKNTKLKYRHSRAGGNLGLSVRKLIGKNG
                     FSNPETPDSCFRGNPVF"
BASE COUNT          302 a          301 c          236 g          211 t
ORIGIN      
        1 tcccgacagg aaacatcatg cacatactga ccgccggcgt ggacgaggca gggcgcgggc
       61 ctttagtcgg cagcgtgttt gccgccgccg tcatccttcc ggaaatattc gacctgcccg
      121 gactgaccga ctccaaaaaa ctcagcgaga aaaaacgcga cgcgcttgcc gaaatgataa
      181 aaaatcaggc ggttgcgtgg cacgttgccg ccgcctcgcc cgaagaaatc gccagcctca
      241 acatcctgca cgccaccatg ctcgcgatga aacgcgccgt tgacggtttg gctgtgcgtc
      301 ccgaaagaat attcatcgac ggcaaccgca ttcccgaaca tttgaacatc cctgccgaag
      361 ccgtcgtcaa aggcgacagc aaaatcatcg aaatctccgc cgcatccgtt ttggcaaaga
      421 ccgcacgcga tgcagaaatg tacgcactgg cgcaacgcca tccccaatac ggtttcgaca
      481 aacacaaagg ttacggcacg aagcagcatc tggaagccct caaacaatac ggcgtgctgc
      541 ccgaacaccg ccgagacttc gcccccgtca gaaacctgct cgcgcagcag gccttgtttt
      601 aaaccggcac aaaaatgccg tctgaacttc agacggcatt ttcagcatcg caacttcaaa
      661 gagaaagaac ccaaaccgtc attcccgcga aaaatagaaa atcaaaaaaa aacctaaaat
      721 ccgtcattcc cgcgcaggcg ggaatccaat ccgtccggtt tccgtttttt ttgaatttca
      781 ggtaacttcc aaaccgccat tcccgcaaaa gcgggaatct agaaactcaa agctgcaaga
      841 atttatcaaa aatgactgaa actcaaaaaa ccggattccc gcgaaaacag gaatccggag
      901 tctcagggtt ggaaaaaccg tttttcccga taagtttccg taccgacaga cctagattcc
      961 cgcctgcacg ggaatgacga tatttcagtt ttgtgttttt gatttttttg tttttgcggg
     1021 aatgacgata tagtggatta acaaaaatca
//
LOCUS       DAXHTR010000224         1049 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.224, whole genome shotgun sequence.
ACCESSION   DAXHTR010000224 DAXHTR010000000
VERSION     DAXHTR010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.224"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..242
                     /locus_tag="ACFNML_002000"
     CDS             <1..242
                     /locus_tag="ACFNML_002000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393307.1"
                     /translation="GETTTYRFLLIRYTTMNPSQYLFIDLNFDKIPDTRLCCFSIFQP
                     TVNLKQPVHAMLQFLFLSADCFDKELKIHFMHLKI"
     gene            350..910
                     /locus_tag="ACFNML_002001"
     CDS             350..910
                     /locus_tag="ACFNML_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA large subunit pseudouridine synthase E"
                     /protein_id="HFC6393308.1"
                     /translation="MKNLIAFNKPYGVICQFSPHEKHKSLKDFINFPGFYPAGRLDTD
                     SEGLLLLTDDGRLQAQITDPKFKHPKTYWAQLEGVPDESRLESLRKGIDLGGFVTRPA
                     SIRILKHGEADSLWERIPPIRVRKTVPDFWIEITISEGKNRQVRRMTAKAGYPCLRLI
                     RVASGRLKLFDLDLKPGEWAYAPFKP"
BASE COUNT          304 a          287 c          213 g          245 t
ORIGIN      
        1 aaggcgagac aacgacgtac cggtttttgt taatccgcta taccacgatg aatccttcgc
       61 aatatctgtt tatcgacctc aattttgaca aaataccgga tacgcgcctt tgttgctttt
      121 ccatcttcca accaactgta aatctcaaac agccggtaca cgccatgctt cagtttcttt
      181 tcctgtcggc ggattgtttc gacaaagaat tgaaaatcca tttcatgcac cttaaaattt
      241 aatctgcatt caaacctttt cactttggaa gcaccattta tcggatgtcc cttcgcaata
      301 aacaaatttt cccgataccg ccgcccattt caacccaaac ccaaaagcta tgaaaaacct
      361 catcgccttc aacaaaccct atggcgttat ctgccaattt tcaccgcacg aaaaacacaa
      421 aagcctcaaa gactttatca attttcccgg cttctacccc gccggacggc tcgacaccga
      481 cagcgagggg ctgctgctgc tgaccgacga cggcaggctt caggcacaaa ttaccgaccc
      541 caaattcaaa caccctaaaa cctactgggc gcaactggag ggcgtacccg acgaaagccg
      601 attggaaagc ctaagaaaag ggatagactt aggcggtttc gttacccgtc cggcaagcat
      661 ccgcatcttg aaacacggag aagcagattc gttatgggag cgcatcccgc cgatacgcgt
      721 ccgcaaaacc gttcccgatt tttggattga aattaccatt tctgagggca aaaaccgcca
      781 agtcaggcga atgaccgcca aggcgggcta tccctgcctg cgtctgatca gagtggcaag
      841 cggcagactg aaactgtttg atttggattt aaaacccggg gaatgggcat acgccccgtt
      901 taaaccataa tcacgtttat ctcatcattt ccacaaaagt gggaatccgg aattttatag
      961 tggattaaca aaaatcagga caaggcgacg aagccgcaga cagtacaaat agtacgaaac
     1021 cgattcactc ggtgcttcag caccttaga
//
LOCUS       DAXHTR010000225          956 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.225, whole genome shotgun sequence.
ACCESSION   DAXHTR010000225 DAXHTR010000000
VERSION     DAXHTR010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..956
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.225"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(137..769)
                     /gene="mtrR"
                     /locus_tag="ACFNML_002002"
     CDS             complement(137..769)
                     /gene="mtrR"
                     /locus_tag="ACFNML_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HFC6393309.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDTEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLENHPDLRRK"
BASE COUNT          220 a          207 c          245 g          284 t
ORIGIN      
        1 agattgagca taaaatttta gtaacctatg ttattgcaaa ggtctcaata tgtattttta
       61 gaagaaggct ttgtgtcatt ttgatgccgt ctgaaagggt ttgttcgttt cagacggcat
      121 tgttaccaag gcttgattat ttccggcgca ggtcgggatg gttttccaag ttgtccatca
      181 ttatcccgat gatgcgcggg gcggttttgc ccaaatcgaa acgttcgcag gaagagaacc
      241 accgccaaat cagcccgtcc aaggttgatt tgatgaagat aactgccgtt tccttgtcca
      301 aatcgtcagc caaatcctga ttttccaccg cttcggtcaa aacggcggta attttctcgc
      361 gccagattgc ctgatgcttg cgggcaatgg cgataacggc ggcgttttgc tccgtgtgtt
      421 cgcattttaa aaacaggatg ttgtggaatt tgtagtagat gtcgttgctt tgcagccgct
      481 cgaaaaagtg cagcagcgtg tggcggaata ccgcccaaga ccctccttcg gcatcttcgg
      541 tatcttgcgc gatgcagttt tcgatgtcgt cgcagatacg ttggaacagc gcgtcgaaca
      601 agtcttcctt atttttgaaa tgccaataga gcgcgccgcg cgttacgccg gcggcttggg
      661 cgatttcgtt gagcgaggtg cgcgcaatcc ctttgcggta aaaggtttcc aaggcggcaa
      721 gcatcaggtg ttctttggtt ttcaaggctt cggttttggt ttttctcata atggcgtttt
      781 cgtttcgggt cggtttgatg agggcggatt ataaaaaaga ctttgtaacc atgcaatcgt
      841 gtatgtataa tgaaacccat gaaattgaga ctacatctca actttgaaaa caggcataac
      901 ctgctttgca acccgttcga acattcgggt atcaaagcag gcggttttta tatcct
//
LOCUS       DAXHTR010000226          887 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.226, whole genome shotgun sequence.
ACCESSION   DAXHTR010000226 DAXHTR010000000
VERSION     DAXHTR010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..887
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.226"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            220..>887
                     /locus_tag="ACFNML_002003"
     CDS             220..>887
                     /locus_tag="ACFNML_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HFC6393310.1"
                     /translation="MFPDKYKLSLEENIFLAKKVLVAQIHNLSRFENCQTTLLQTEQI
                     IHGKNVASASLEDIQTILNLKRAYQYVISHISNGEPVDISLLKKINNIVAKDDSLAPG
                     DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEAAVRFMLYCMRQQVF
                     WDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRSGDDTDISKFVYQNC
                     ISGI"
BASE COUNT          253 a          195 c          203 g          236 t
ORIGIN      
        1 acggatgaaa tcaaccgata ttcaaacaca gtcattttta gcgcattttc agcgtatcgt
       61 taatgcggaa aatttcgtga acaggttttt tgcacaggtc tccgcctcgt ttcgcgggat
      121 gggaaaccgt attagaaaac ggacgcacgc aacataaacc ccgaaagtga tgataagatg
      181 atgatttaac gtactgcttt aattatttaa ggaattatcg tgtttcccga caaatacaag
      241 ttgagtttgg aagaaaatat ttttctggca aagaaagtat tggttgccca aattcacaac
      301 ctcagccgtt ttgagaattg tcagacgacc ttgttgcaga ccgaacaaat tatccatggc
      361 aaaaatgtag cctccgcgtc actggaagac atccaaacca tcttgaacct gaaacgtgcc
      421 tatcaatatg tgatttcgca tatttcaaac ggcgaaccgg tcgatatttc actccttaaa
      481 aaaatcaaca acattgttgc caaggacgat tctttggcac ccggtgattt ccgtaccggt
      541 tcggtcggcg taacgctatt ggacggttcc cgtcatgccc caaatccggt gaaggaaatt
      601 gaagtggctc gcgtgttaca aaatatcgga ctgcaaagcg gttcgacgac ggaggcagcc
      661 gtccgtttta tgctttattg tatgcggcag caggtttttt gggacggcaa caaacgaacg
      721 gcaaccttat ttgccaacgg tctgatgatg gcggggggct gcggcatctt ggaaatctcc
      781 gaaatgcaga tgccgcaatt caatgaaaaa ctgtccgcat tctatcgctc cggcgacgat
      841 accgatattt ccaagtttgt gtatcaaaat tgtatatcgg gcataga
//
LOCUS       DAXHTR010000227          767 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.227, whole genome shotgun sequence.
ACCESSION   DAXHTR010000227 DAXHTR010000000
VERSION     DAXHTR010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.227"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(90..641)
                     /locus_tag="ACFNML_002004"
     CDS             complement(90..641)
                     /locus_tag="ACFNML_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393311.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDLEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
BASE COUNT          219 a          284 c          141 g          123 t
ORIGIN      
        1 aaagaaaccg ccattagcac tacacccccc caagctgcgg ctgccaaaca catcggctgc
       61 ttctcgacgc acccgctgaa aaatgcgtcc tatccgtccg ccttgcccgc accccctgac
      121 tttgccgccc tgcccgatgc cgtctgaagc ctgaaccgcc ccaccagccc gatgacgcac
      181 gacaccgccg gaaagggcat acacaaagcc gtcagcacat aacgcgcatt ccccgtattg
      241 accacgtccg cgcccttcag cagcagcggt agcgcgttcg cccactccca caccgacggc
      301 gcgctgaacg ccaataccgc caccagcccc aacatccgga acaccccgat ttccaacacc
      361 tgccgccgcc gcaccgaacg gttgagcaaa aacacaaccg ccaacccgaa acagaccacc
      421 gccgccatac cgccgttggc gacaatctgc aaactgttga atgcaatatc gggcgtaacc
      481 gccaacacgc cttccaaatc cggcacggca cgctgcaaat ccaaaccctg caacacattc
      541 aacacaaaac cgtagatcaa atcaaaaaac accacgccta tcggcaaagc actcaaaacc
      601 cgccacatct tcctgtccga ccaaccgttc aaaaccttca ttttacccga ccacgcaagc
      661 cgccgaacaa aaaataaggg gctgtcctag ataactagga caaacttgat tttactaatt
      721 gttttaaaat ggaacaagaa cttttatctc actgttgtta aaacgtc
//
LOCUS       DAXHTR010000228          761 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.228, whole genome shotgun sequence.
ACCESSION   DAXHTR010000228 DAXHTR010000000
VERSION     DAXHTR010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.228"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(38..604)
                     /locus_tag="ACFNML_002005"
     CDS             complement(38..604)
                     /locus_tag="ACFNML_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HFC6393312.1"
                     /translation="MGFYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGT
                     VEALTPLAGWVGGFYAKPFISEWDHWVAFVLLGGLGLKMMREGLSGEAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRCAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          217 a          259 c          151 g          134 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactatattt tccgacatca ttgaatcaaa cccaaatgcg
       61 acaagagcgt ccatgtgccg atggcaatca acaccaaacc tccggcaaat tccgcacacc
      121 tgccgaacaa tacgcccaaa gcccttcccg ccgtcagccc gaccgccacc atcaccgtcg
      181 tcgccatacc gatgattgcg gcggcaaagg cgatgtttac ctccataaac gccaagccca
      241 ccccgactat catggaatca atactggttc caaaagcagt caaaaccgtc atccataggc
      301 tttcccgttt gctttcgcgc acatcttccg cctcgccgga cagcccttcg cgcatcattt
      361 tcagacccag cccgcccagc aggacgaaag ccacccaatg gtcccattcg ctgataaacg
      421 gcttggcata aaaaccgcct acccagcctg ccagcggcgt gagcgcttca accgtgccga
      481 acaccaaagc cgttgccgca attttgcgcg gaggcattct gaccgccgcg ccctttgcca
      541 atgcgacggc aaacgcatcc atcgacatcc ccagagcaat caagagcaaa gcataaaaac
      601 ccataccgca cccgtcctca aaaagggcgg attatagcaa aagcaaaaaa atgcaaaaat
      661 gccgcacgaa aacccgcatc ccgtcattcc cgcaaaaaca aaaaatcaaa aacagaaatc
      721 ccgtcattcc cgcgcaggcg ggaatccaga gttgtcggtg c
//
LOCUS       DAXHTR010000229          705 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.229, whole genome shotgun sequence.
ACCESSION   DAXHTR010000229 DAXHTR010000000
VERSION     DAXHTR010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.229"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..85)
                     /locus_tag="ACFNML_002006"
     CDS             complement(<1..85)
                     /locus_tag="ACFNML_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HFC6393313.1"
                     /translation="MLPSKYEDNALQIKEKSTERTAPFFCTR"
     gene            complement(189..>705)
                     /locus_tag="ACFNML_002007"
     CDS             complement(189..>705)
                     /locus_tag="ACFNML_002007"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393314.1"
                     /translation="NQCCPRIKNKIGGSLQPHQMPSEHVFGRRFVGGYRSGTSEKGFQ
                     AVFGRVVTVENVIRLPEKFGRFSGSLWYWAQQTQVQIRRCAVIVRMPIQPKQTSVFRK
                     WMFDGAKVVRRMEGNLWVFGMVALEKTCFKGQMPSENRFQTAFSVYLKQTGKGSNILQ
                     SSYSHKRFIVN"
BASE COUNT          182 a          185 c          120 g          218 t
ORIGIN      
        1 ttcgagtaca aaaaaacgga gcagttcttt ctgtactttt ttctttaatt tgcagtgcgt
       61 tatcttcata tttcgagggt aacatatctg ctaatctagt acagccccta gtttaaagct
      121 ttgtttctta agtccgcaga gtatgccatg gttagacctt caaagttgag tattgtacta
      181 ttttgttttt aattaactat aaaacgcttg tgtgaatagg aagactgcag aatattgctg
      241 ccttttcctg tttgctttaa ataaacagaa aatgccgtct gaaaccgatt ttcagacggc
      301 atttgtccct taaaacacgt tttttcaagc gcgaccatac caaacaccca caggttgcct
      361 tccatccttc gtacaacctt tgctccatca aacatccact tcctaaatac tgatgtctgc
      421 ttaggttgaa tcggcattcg tacgattacg gcacaccgcc taatctgtac ctgcgtctgt
      481 tgcgcccaat accaaaggct acctgaaaat ctcccaaact tttcaggtag ccttatcacg
      541 ttttcgactg tcaccacacg cccaaagacc gcctgaaacc ctttttcaga cgttccactc
      601 cgataccccc caacaaaacg ccgcccgaac acatgttcag acggcatttg atgcggctgc
      661 agacttcccc ctattttatt ttttatccgc gggcagcact ggttt
//
LOCUS       DAXHTR010000230          703 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.230, whole genome shotgun sequence.
ACCESSION   DAXHTR010000230 DAXHTR010000000
VERSION     DAXHTR010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.230"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            26..703
                     /locus_tag="ACFNML_002008"
     CDS             26..703
                     /locus_tag="ACFNML_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISNme3 family transposase"
                     /protein_id="HFC6393315.1"
                     /translation="MKLKNKYQKFSKISEQKFREIIRCFALDLTASDTAKMTGISVRG
                     INPIFLKIRHRIAALCEQSSPLSGVVELDESYFGARRIRGKRGRGASGKTIVFGILKR
                     NGVVYTEIVPDASKASLIKVIRGHISADSEINTDGWKAYDGLVDMGYEKHYRVHHGSN
                     EFARGKQHINGIESFWSYTKGRLAKFHGISKEMFYLHLKETEFRFNHRHEDLGKILMK
                     MLRNNPI"
BASE COUNT          217 a          136 c          156 g          194 t
ORIGIN      
        1 tcaaaaaacc tgataagctc ctaatatgaa actaaaaaat aagtatcaaa agttcagcaa
       61 aatttcagag caaaagttta gagaaataat ccgctgtttc gctcttgatt tgaccgcttc
      121 cgatactgct aaaatgaccg gcattagtgt acgcggtatc aatcctattt tcctgaaaat
      181 cagacacagg attgctgctc tgtgcgaaca aagctcacca ctgtccggtg tggtcgaatt
      241 agatgaatct tactttggtg cgcgacgtat caggggtaag cgcggtcgcg gagcgtcagg
      301 taaaaccatc gtatttggca tactgaaacg caatggcgtg gtctatacgg aaatcgttcc
      361 cgacgcttcg aaagcctcac taatcaaggt catacgtggt cacatatctg ctgacagtga
      421 aatcaatact gacggctgga aagcatatga cggtcttgtc gatatgggct atgagaagca
      481 ttaccgtgtc catcatggtt ccaatgagtt tgctcgagga aagcaacata tcaatggtat
      541 tgaatctttt tggagttata ctaaggggcg tttggctaaa tttcatggta tttccaaaga
      601 gatgttttat ctgcatctca aagaaacgga gtttaggttt aaccaccggc atgaagattt
      661 aggtaaaata ttaatgaaga tgcttcgaaa taacccaatt taa
//
LOCUS       DAXHTR010000231          671 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.231, whole genome shotgun sequence.
ACCESSION   DAXHTR010000231 DAXHTR010000000
VERSION     DAXHTR010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.231"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            60..371
                     /gene="rpsJ"
                     /locus_tag="ACFNML_002009"
     CDS             60..371
                     /gene="rpsJ"
                     /locus_tag="ACFNML_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFC6393316.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHMNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            492..>671
                     /locus_tag="ACFNML_002010"
     CDS             492..>671
                     /locus_tag="ACFNML_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6393317.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSM"
BASE COUNT          186 a          179 c          140 g          166 t
ORIGIN      
        1 cgtaccgtgg gtgccggcgt ggtttcttct gttatcgctt aattgaagga tattgataaa
       61 tggcaaacca aaaaatccgt atccgcctga aagcttatga ttacgccctg attgaccgtt
      121 ctgcacaaga aatcgttgaa actgcaaaac gtaccggtgc cgttgtaaaa ggtccgattc
      181 cgttgccgac caaaatcgaa cgtttcaaca ttctgcgttc tccgcacatg aacaaaactt
      241 cccgtgaaca attggaaatc cgcacccact tgcgcctgat ggacatcgtg gattggaccg
      301 ataaaactac cgatgcgctg atgaagctgg atttgccggc cggtgttgat gtagaaatca
      361 aagtccaata attcggacta ttgaaaatcc ccaagcaatc aatgcttggg gattttttat
      421 gttatgccga gacctttgca aaattcccca aaatccccta aattcccacc aagacattta
      481 ggggatttct catgagcacc ttcttccaac aaaccgccca agccatgatt gccaaacaca
      541 tcgaccgttt cccgctattg aagttggacc aggtgattga ttggcagccg atcgaacaat
      601 acctgaaccg tcaaaaaaac cgttaccttc gagaccaccg cggccgtccc gcctacccgc
      661 tgttgtccat g
//
LOCUS       DAXHTR010000232          665 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.232, whole genome shotgun sequence.
ACCESSION   DAXHTR010000232 DAXHTR010000000
VERSION     DAXHTR010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..665
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.232"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..419)
                     /locus_tag="ACFNML_002011"
     CDS             complement(<1..419)
                     /locus_tag="ACFNML_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HFC6393318.1"
                     /translation="MKVQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEE
                     VLKKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFS
                     VLRGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGN"
     gene            complement(364..554)
                     /locus_tag="ACFNML_002012"
                     /pseudo
     CDS             complement(364..554)
                     /locus_tag="ACFNML_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          169 a          190 c          129 g          177 t
ORIGIN      
        1 aaatttccct cacgggtttg ccaagcatcc aaaaactgcg ccctgctcat cgaaacatga
       61 cccagcgacg ggtcggcaag caaaaccgta ttgccgtcta taccgcgcaa taccgagaaa
      121 tgatcatcct tacggtattt cagatacacg atgacgggga ttttcaactg cgcgagctgc
      181 tcgaaagaca gggcatagcc tttcgcttca aaacccaaat ctggcataat gcgccgcata
      241 tcctcaaacg acgcgcgcat ctgctcctta tccagctttt tcaacacttc ttcttccgtc
      301 agcgtttgcc cgtaaaaatt gttcaaaagc gtcgccaccg aagccgcccc gcaggaaaaa
      361 tccaaatcct gctttacaat attgaaatcg cgcctttctt tccaactctg cactttgatt
      421 tttccataag caacaggatt atagtggatt aaatttaaac cagtacggcg ttgcctcgcc
      481 ttgccgtact atctgtactg tctgcggttt cgtcgccttg tcctgatttt tgttaatcca
      541 ctataggttt ccgtgcggac gtgttcagat tcccgccttc gctggaataa cggcggagca
      601 gtttctgctt ttccgataaa tgaccgtaac ttaaaatccc gtcatcccca cgaaagcaaa
      661 aatcc
//
LOCUS       DAXHTR010000233          651 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.233, whole genome shotgun sequence.
ACCESSION   DAXHTR010000233 DAXHTR010000000
VERSION     DAXHTR010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.233"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(51..536)
                     /locus_tag="ACFNML_002013"
     CDS             complement(51..536)
                     /locus_tag="ACFNML_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFC6393319.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAAGFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          194 a          212 c          116 g          129 t
ORIGIN      
        1 tcaaaccgga aaaatgccgt ctgaatcttc agacggcatt ccattcatat ttattcgtct
       61 tttttgtgtt cgatggcgtt tcgggaacga ttgccgccgt aaggctcttc aaccgtatat
      121 tccccctcga taatatcgtc atcgcgggaa aagccctctt ttctgcccga ttggttcatg
      181 ttgaaaaaat tttccgcacc tcctgcctgc aacactgcac ctcccttaaa cggcagcagc
      241 agcaacaccg ccaacaccga ggatacgaat cccggactca tcagacacac cgccgccacc
      301 gtataacgga taggccacaa catctgataa acggataccc tcccgccgct tctcattgcc
      361 gcgcccgcca ataaaagacc ggacagcccc gtatgcctga gcatcagcac accggcggca
      421 aaacctgccg ccatcaaaaa caacgtccag ccgccgccca accaatcggc aacccacaca
      481 atcgacataa tctccaaaaa cagcagcacc aaaaaaccga taccgaaaaa tctcattgac
      541 cgtcatcctt atatttaagt aaacagcaaa ccgcccgaac aggactccaa gcgacctgcc
      601 tgtaaatgat tacaaaacca tgtgcttcaa gccgaaacaa tgtgaaatca c
//
LOCUS       DAXHTR010000234          626 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.234, whole genome shotgun sequence.
ACCESSION   DAXHTR010000234 DAXHTR010000000
VERSION     DAXHTR010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.234"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            63..494
                     /locus_tag="ACFNML_002014"
     CDS             63..494
                     /locus_tag="ACFNML_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HFC6393320.1"
                     /translation="MPTRLDSRLRGNDEAILLAEGQKSAVTEYYLNHGIWPGGNSDAG
                     VASSSTIKGKYVQKVEVNNGVVTATMLSSGVNKEIKGKKLSLWAKRQNGSVKWFCGLP
                     VERTAPTTTNDDVKADTAANGKQIDTKHLPSTASTRKSTPN"
BASE COUNT          188 a          182 c          147 g          109 t
ORIGIN      
        1 tcccgcaaaa gcgggaatcc agaacgcaaa atctcaagaa accgttttcc cgataagttt
       61 ccgtgccgac gaggctggat tcccgcctgc gcgggaatga cgaagctatc cttttggctg
      121 aaggtcaaaa atcagccgtc accgagtatt acctgaatca cggcatatgg cccggcggca
      181 acagtgatgc cggcgtggca tcttcttcaa caatcaaagg caaatatgtt cagaaagttg
      241 aagtcaacaa cggcgtcgtt accgccacaa tgctttcaag cggcgtaaac aaagaaatca
      301 aaggcaaaaa actctccctg tgggccaagc gtcaaaacgg ttcggtaaaa tggttctgcg
      361 gactgccggt tgagcgcacc gcccccacca ccaccaacga cgacgtcaaa gccgacaccg
      421 ccgccaacgg caagcagatc gacaccaagc acctgccgtc aacagcatcg acaagaaaat
      481 cgacaccaaa ctagagaaac ctttttaccc gataagtttc cgtaccgacg gggctggatt
      541 cccgccggcg cgggaatgac gaagctatcc atacggaaat ctacatcccg tcattcccac
      601 gaaagtggga atccagaacg taaaat
//
LOCUS       DAXHTR010000235          606 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.235, whole genome shotgun sequence.
ACCESSION   DAXHTR010000235 DAXHTR010000000
VERSION     DAXHTR010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.235"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            40..414
                     /locus_tag="ACFNML_002015"
     CDS             40..414
                     /locus_tag="ACFNML_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HFC6393321.1"
                     /translation="MDEKQKIKILDFQIDLSSIFNSYKNQMGINIQDKNLKNNFCSFM
                     EELLNDGSIRLHDYTDGIGIPLTGTSKEQVQKLKDIWPTLEDAQAIWPEDPWYYLEWL
                     WWDIACPIDLADLPNIDIYEQA"
BASE COUNT          197 a          107 c          116 g          186 t
ORIGIN      
        1 ttggagctta aattccaata atttacaaag gattttaccg tggatgagaa acaaaaaatt
       61 aagattcttg attttcaaat cgatttatcc tcaattttta actcttataa aaatcaaatg
      121 ggtattaata ttcaagataa aaacttaaaa aacaatttct gttcttttat ggaagaactg
      181 ttaaatgacg gttcaatccg tttacatgat tataccgacg gtatcggaat tcctctaact
      241 ggaacttcaa aagaacaagt gcagaaattg aaagacatat ggcctacttt ggaagatgcc
      301 caagcaatat ggcctgaaga cccttggtat tacttagaat ggctttggtg ggatattgcg
      361 tgtccaatag atttggccga tttgccgaat attgatattt atgagcaagc gtaggtatgg
      421 ttagccgcct ttagcggcgt aaccgtacgc atatcagcaa actttataaa ataacaaggc
      481 cgtctgaaat ctgtttttca actttttcag acggccttgc aacttggcat ttcattcgta
      541 cggttacgcc gctaaaggcg gctaaccgta cctacgagct ctgataaaaa tgatttatgg
      601 aagcaa
//
LOCUS       DAXHTR010000236          602 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.236, whole genome shotgun sequence.
ACCESSION   DAXHTR010000236 DAXHTR010000000
VERSION     DAXHTR010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.236"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..232
                     /locus_tag="ACFNML_002016"
     CDS             <1..232
                     /locus_tag="ACFNML_002016"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393322.1"
                     /translation="DTGTPRADFKHFPRKRKFLKIKFKISHPTHPMLPRKFKNLKFRP
                     NQHTRCPMPIEIPHANKKVPKIGTKTDNFRHC"
     gene            464..>602
                     /gene="pilE"
                     /locus_tag="ACFNML_002017"
     CDS             464..>602
                     /gene="pilE"
                     /locus_tag="ACFNML_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HFC6393323.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     LL"
BASE COUNT          214 a          163 c           87 g          138 t
ORIGIN      
        1 ggacacagga acaccacgcg ccgatttcaa acactttcca agaaaacgga aatttttaaa
       61 aattaaattt aaaatttccc acccaaccca ccctatgcta ccgcgcaaat tcaaaaatct
      121 caaattccga cccaatcaac acacccgatg ccccatgcca atagagatac cccacgccaa
      181 taaaaaagtg ccgaaaatcg gcactaaaac tgacaatttt cggcactgct gacaaaaaac
      241 ggtaaccccc tgcttcctca aaccacaccc aaacgaaaga aaatgtaaaa taaaaacaac
      301 cacataaaga taaacgcata aattttcacc tcaaaacaca aacttgatac caatcttgct
      361 ttataataca caggtatcgc aacaaaatac cgatggtcaa atacattgca taatgccgat
      421 ggcgtaagct tgaggcattt cctttccaat taggagtaat tttatgaaca cccttcaaaa
      481 aggttttacc cttatcgagc tgatgattgt gattgccatc gtcggcattt tggcggcagt
      541 cgcccttcct gcttatcaag actacacagc ccgcgcacaa gtttccgaag ccattctttt
      601 ag
//
LOCUS       DAXHTR010000237          597 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.237, whole genome shotgun sequence.
ACCESSION   DAXHTR010000237 DAXHTR010000000
VERSION     DAXHTR010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.237"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            172..465
                     /locus_tag="ACFNML_002018"
     CDS             172..465
                     /locus_tag="ACFNML_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFC6393324.1"
                     /translation="MKTNDKLNRQIDVLQKQSAAIHNEAYIEMNTLLYRHREVVSVHN
                     RKADYAEKGKERIALFPRGLNGITKLPAAVLLPERPDCFDMKAVWQMFNRIPR"
BASE COUNT          158 a          150 c          160 g          129 t
ORIGIN      
        1 tccattcggc agggaaagcc tgaagaagaa ttttcccgta agatggccgg ctgcctgacc
       61 gacttggaaa actgtttgga aaaatacctc gaacagttcg ggccggtatc ggaaagtatc
      121 gaagcctgta ccgtcaaact gcaggagtaa ccgtcgttct tcaacaggct gatgaaaacc
      181 aacgacaagc tcaataggca gattgacgtg ttgcagaaac agtcggcagc catccacaac
      241 gaagcctata tcgaaatgaa cacgctgctt taccgccacc gcgaagtggt ttccgtccac
      301 aaccgcaagg cggattatgc ggaaaagggc aaagagcgga ttgcgctgtt cccgcgcggc
      361 ttgaacggca tcaccaagct gcctgccgcc gtcctgttgc ccgagcgtcc cgattgtttt
      421 gatatgaagg cagtttggca aatgtttaat agaataccga ggtaggcgta tcggctgccg
      481 gataaaaatg ccgtctgaaa cggaagcgtt cagacggcat tgttgcagtg gaacaaatca
      541 aaatgcaccc cgtcattcct gtctgtacgg tgatagcggc agtgttttcc tgcttcg
//
LOCUS       DAXHTR010000238          587 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.238, whole genome shotgun sequence.
ACCESSION   DAXHTR010000238 DAXHTR010000000
VERSION     DAXHTR010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.238"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..489
                     /locus_tag="ACFNML_002019"
     CDS             <1..489
                     /locus_tag="ACFNML_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="opacity family porin"
                     /protein_id="HFC6393325.1"
                     /translation="RKWKESNYSKKVTEFKHQNGTKKEDKTEHQGNGSFHAASSLGLS
                     AIYDFKLNDKFKPYIGARVAYGHVRYSIDSTKKTTDVVTVATNTPDGAPTIYNEPKTQ
                     NAYHESHSIRRLGLGVVAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRY
                     RF"
BASE COUNT          177 a          168 c          121 g          121 t
ORIGIN      
        1 agaaaatgga aagaaagtaa ttattctaaa aaagttactg aatttaaaca ccaaaacggc
       61 accaaaaaag aagacaaaac agaacatcaa ggaaacggca gcttccacgc cgcttcttct
      121 ctcggcttat ccgccattta cgatttcaaa ctcaacgata aattcaaacc ctatatcggt
      181 gcgcgcgtcg cctacggaca cgtcagatac agcatcgatt cgaccaaaaa aacaacagat
      241 gttgttaccg tcgccaccaa tactcctgac ggagcaccta cgatttataa tgagccaaag
      301 acgcaaaacg cctatcacga aagccacagc atccgccgct tgggtcttgg tgtcgtcgcc
      361 ggtgtcggtt tcgacatcac gcccaagctg actttagaca ccggataccg ttaccacaac
      421 tggggacgct tggaaaacac ccgcttcaaa acccacgaag tctcattggg catgcgctac
      481 cgcttctgat tccccgacac cgatgccgtc tgaaccttca gacggcattt ttgattcacc
      541 tgccgtttac agacgcgggg cgggcgtgga aatacccgaa ccgtcat
//
LOCUS       DAXHTR010000239          582 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.239, whole genome shotgun sequence.
ACCESSION   DAXHTR010000239 DAXHTR010000000
VERSION     DAXHTR010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.239"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            32..394
                     /locus_tag="ACFNML_002020"
     CDS             32..394
                     /locus_tag="ACFNML_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HFC6393326.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEKWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKETFAKFPKIP"
     gene            425..>582
                     /locus_tag="ACFNML_002021"
     CDS             425..>582
                     /locus_tag="ACFNML_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HFC6393327.1"
                     /translation="MSTFFRQTAQAMIAKHINRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRP"
BASE COUNT          175 a          166 c          128 g          113 t
ORIGIN      
        1 cgcacaaatt tagtcaatat caagaccaat tatgaaccaa ctcgaccaac ttggcacccg
       61 tatcaacctg atttgcaatg tcttcgacaa atggatcggg cagcaggatc tgaattacaa
      121 cctctttgcc gtactttata ccctggcaac cgaaggcagc cgcacgcaaa agcatatcgg
      181 cgaaaagtgg agcctgccca aacagaccgt ttcaggcgta tgcaaaaccc ttgccggaca
      241 agggttgatt gaatggcagg aaagcgaaca ggaccggcgc gaacggttgc tgtcgctgac
      301 cgaaaaaggc aaagcccatg ccgcaccttt aacagaaagc gcgcaggaat tcagcgacaa
      361 agagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat ttaggggatt
      421 ttccatgagc accttcttcc ggcaaaccgc ccaagccatg attgccaaac acatcaaccg
      481 cttcccgcta ttgaagttgg accaggtgat tgattggcag ccgatcgaac agtatctgaa
      541 tcgtcaaaga acccgttacc ttagagacca ccgcggccgt cc
//
LOCUS       DAXHTR010000240          544 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.240, whole genome shotgun sequence.
ACCESSION   DAXHTR010000240 DAXHTR010000000
VERSION     DAXHTR010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.240"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            <1..>544
                     /locus_tag="ACFNML_002022"
     CDS             <1..>544
                     /locus_tag="ACFNML_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HFC6393328.1"
                     /translation="TGAGAAIIALAVTVVTSGAGTGAVLGLNGAAAAATDAAFASLAS
                     QASVSFINNKGDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWINN
                     LTVNLANAGSAALINTAVNGGSLKDNLEANILAALVNTAHGEAASKIKQLDQHYIVHK
                     IAHAIAGCAAAAANKGKCQDG"
BASE COUNT          143 a          150 c          158 g           93 t
ORIGIN      
        1 taaccggagc cggagccgca attatcgcac tggccgttac cgtggtcacc tcaggcgcag
       61 gaaccggagc cgtattggga ttaaacggtg cggccgccgc cgcaaccgat gcagcattcg
      121 cctctttggc cagccaggct tccgtatcgt tcatcaacaa caaaggcgat gtcggcaaaa
      181 ccctgaaaga gctgggcaga agcagcacgg tgaaaaatct ggtggttgcc gccgctaccg
      241 caggcgtagc cgacaaaatc ggcgcttcgg cactgaacaa tgtcagcgat aagcagtgga
      301 tcaacaacct gaccgtcaac ctagccaatg cgggcagtgc cgcactgatt aataccgctg
      361 tcaacggcgg cagcctgaaa gacaatctgg aagcgaatat ccttgcggct ttggtgaata
      421 ctgcgcatgg agaagcagcc agtaaaatca aacagttgga tcagcactac atagtccaca
      481 agattgccca tgccatagcg ggctgtgcgg cagcggcggc gaataagggc aagtgtcagg
      541 acgg
//
LOCUS       DAXHTR010000241          525 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.241, whole genome shotgun sequence.
ACCESSION   DAXHTR010000241 DAXHTR010000000
VERSION     DAXHTR010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.241"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            4..234
                     /locus_tag="ACFNML_002023"
     CDS             4..234
                     /locus_tag="ACFNML_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6393329.1"
                     /translation="MAQKEFEEFVNNVKEYDILESFSYFLNKRVAHNIWKIYFSDESV
                     IFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
     gene            176..>525
                     /locus_tag="ACFNML_002024"
     CDS             176..>525
                     /locus_tag="ACFNML_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6393330.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLA"
BASE COUNT          173 a           92 c          101 g          159 t
ORIGIN      
        1 ttaattgctc agaaagaatt tgaagaattt gttaataacg taaaagagta cgatatttta
       61 gagagtttct cttatttctt aaataagaga gtcgcacata atatttggaa gatttatttt
      121 tctgatgaat ctgttatttt tataagaaaa tcagaagaga acggaaaaac tgttcatgaa
      181 ttcgtttatc aagagtacac cgatagttct gattttaatt ctatgttcga gtaattctta
      241 ttctgaacct gtaagattag aaagatcaca aattaaattt caatcaagta ataatttaaa
      301 atcaagtggt tttaaattag attcaagttc taagagtttt gccaaattca tagaagcggc
      361 aaattttgag cacatcccta cgggcgcaaa agcccgaatc aacgccaaaa taaccgccag
      421 cgtttcccgc gccggcgtat tggcgggggt cggcaaactt gcccgcttag gcgcgaaatt
      481 cagcacaagg gcggttccct atgtcggaac agccctttta gccca
//
LOCUS       DAXHTR010000242          522 bp    DNA     linear   BCT 02-OCT-2024
DEFINITION  TPA_asm: Neisseria meningitidis isolate NM000000140
            SAMN43432231-rid23052293.denovo.242, whole genome shotgun sequence.
ACCESSION   DAXHTR010000242 DAXHTR010000000
VERSION     DAXHTR010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43432231
            Sequence Read Archive: SRR30498663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-AUG-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-AUG-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002346378.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002346378.1
            Genome Coverage       :: 364x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/28/2024 07:33:56
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 2,026
            CDSs (total)                      :: 1,973
            Genes (coding)                    :: 1,864
            CDSs (with protein)               :: 1,864
            Genes (RNA)                       :: 53
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 109
            CDSs (without protein)            :: 109
            Pseudo Genes (ambiguous residues) :: 0 of 109
            Pseudo Genes (frameshifted)       :: 60 of 109
            Pseudo Genes (incomplete)         :: 57 of 109
            Pseudo Genes (internal stop)      :: 10 of 109
            Pseudo Genes (multiple problems)  :: 16 of 109
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43432231-rid23052293.denovo.242"
                     /isolate="NM000000140"
                     /isolation_source="patient"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /geo_loc_name="USA: NY, New York City"
                     /lat_lon="40.71 N 74.01 W"
                     /collection_date="2024"
                     /collected_by="NYC DOHMH PHL"
     gene            complement(<1..305)
                     /locus_tag="ACFNML_002025"
     CDS             complement(<1..305)
                     /locus_tag="ACFNML_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgG-binding virulence factor TspB family
                     protein"
                     /protein_id="HFC6393331.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLA"
     gene            complement(289..>522)
                     /locus_tag="ACFNML_002026"
     CDS             complement(289..>522)
                     /locus_tag="ACFNML_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HFC6393332.1"
                     /translation="VIAQSEFVEFAKINKSENVIDCFSFFWNRRIKHDIWLISFSDNS
                     EKVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
BASE COUNT          153 a          104 c           93 g          172 t
ORIGIN      
        1 tgggctaaaa gggctgttcc gacataggga accgcccttg tgctgaattt cgcgcctaag
       61 cgggcaagtt tgccgccccc cgccaatacg ccggcgcggg aaatgctggc ggttattttg
      121 gcgttgattc gggcttttgc gcccgtaggg atgtgtgtta aatctaccgt ttttattaaa
      181 tcagatgaat aagttttact atttttaggt acaaacttat gaattttcgc accttgtccg
      241 gtatcaactg aaacagtttc agatattttt actgcatttg cattcgcttc aaacgaatac
      301 atcatcaaaa ttgcaattat cgacaatttc gcaaaattca aatttgtata ttttatgacc
      361 atctttcagg gattctttaa ttaccttttc tgaattatca gaaaatgaga ttagccaaat
      421 atcatgttta attcttctat tccagaaaaa agagaaacaa tcaataacat tttcagactt
      481 attaatcttc gcaaattcaa caaattcaga ttgcgctata ac
//
